LOCUS       DAVMHG010000001        63188 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMHG010000001 DAVMHG010000000
VERSION     DAVMHG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.001"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            327..1019
                     /locus_tag="VYR08_000001"
     CDS             327..1019
                     /locus_tag="VYR08_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3465602.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            1073..1462
                     /locus_tag="VYR08_000002"
     CDS             1073..1462
                     /locus_tag="VYR08_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3465603.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(2123..3151)
                     /gene="queA"
                     /locus_tag="VYR08_000003"
     CDS             complement(2123..3151)
                     /gene="queA"
                     /locus_tag="VYR08_000003"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV3465604.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            3303..4010
                     /locus_tag="VYR08_000004"
     CDS             3303..4010
                     /locus_tag="VYR08_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV3465605.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            4150..4326
                     /locus_tag="VYR08_000005"
     CDS             4150..4326
                     /locus_tag="VYR08_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV3465606.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            4456..5391
                     /locus_tag="VYR08_000006"
     CDS             4456..5391
                     /locus_tag="VYR08_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV3465607.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5384..6172
                     /locus_tag="VYR08_000007"
     CDS             5384..6172
                     /locus_tag="VYR08_000007"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV3465608.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            6173..6556
                     /locus_tag="VYR08_000008"
     CDS             6173..6556
                     /locus_tag="VYR08_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV3465609.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            6572..6961
                     /locus_tag="VYR08_000009"
     CDS             6572..6961
                     /locus_tag="VYR08_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3465610.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            7045..8175
                     /locus_tag="VYR08_000010"
     CDS             7045..8175
                     /locus_tag="VYR08_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV3465611.1"
                     /translation="MQKKTTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8180..8917
                     /locus_tag="VYR08_000011"
     CDS             8180..8917
                     /locus_tag="VYR08_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV3465612.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRCFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEELISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            8928..9701
                     /locus_tag="VYR08_000012"
     CDS             8928..9701
                     /locus_tag="VYR08_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV3465613.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEVIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            9691..10308
                     /locus_tag="VYR08_000013"
     CDS             9691..10308
                     /locus_tag="VYR08_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV3465614.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGGVIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            10518..10919
                     /locus_tag="VYR08_000014"
     CDS             10518..10919
                     /locus_tag="VYR08_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV3465615.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            10919..11197
                     /locus_tag="VYR08_000015"
     CDS             10919..11197
                     /locus_tag="VYR08_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV3465616.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            11187..11960
                     /locus_tag="VYR08_000016"
     CDS             11187..11960
                     /locus_tag="VYR08_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV3465617.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            11961..13265
                     /locus_tag="VYR08_000017"
     CDS             11961..13265
                     /locus_tag="VYR08_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV3465618.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADCLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            13839..14717
                     /gene="pstS"
                     /locus_tag="VYR08_000018"
     CDS             13839..14717
                     /gene="pstS"
                     /locus_tag="VYR08_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV3465619.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKRLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            14717..15634
                     /gene="pstC"
                     /locus_tag="VYR08_000019"
     CDS             14717..15634
                     /gene="pstC"
                     /locus_tag="VYR08_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3465620.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            15624..16508
                     /gene="pstA"
                     /locus_tag="VYR08_000020"
     CDS             15624..16508
                     /gene="pstA"
                     /locus_tag="VYR08_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3465621.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVIHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            16519..17322
                     /gene="pstB"
                     /locus_tag="VYR08_000021"
     CDS             16519..17322
                     /gene="pstB"
                     /locus_tag="VYR08_000021"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3465622.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            17335..18093
                     /gene="pstB"
                     /locus_tag="VYR08_000022"
     CDS             17335..18093
                     /gene="pstB"
                     /locus_tag="VYR08_000022"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3465623.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            18105..18758
                     /gene="phoU"
                     /locus_tag="VYR08_000023"
     CDS             18105..18758
                     /gene="phoU"
                     /locus_tag="VYR08_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3465624.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            18915..19730
                     /locus_tag="VYR08_000024"
                     /pseudo
     CDS             18915..19730
                     /locus_tag="VYR08_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(19750..21021)
                     /locus_tag="VYR08_000025"
     CDS             complement(19750..21021)
                     /locus_tag="VYR08_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV3465625.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            21163..21894
                     /gene="budA"
                     /locus_tag="VYR08_000026"
     CDS             21163..21894
                     /gene="budA"
                     /locus_tag="VYR08_000026"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV3465626.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            21904..22656
                     /locus_tag="VYR08_000027"
     CDS             21904..22656
                     /locus_tag="VYR08_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV3465627.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            22768..23673
                     /gene="murB"
                     /locus_tag="VYR08_000028"
     CDS             22768..23673
                     /gene="murB"
                     /locus_tag="VYR08_000028"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV3465628.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            23833..24990
                     /locus_tag="VYR08_000029"
     CDS             23833..24990
                     /locus_tag="VYR08_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465629.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            24971..25777
                     /locus_tag="VYR08_000030"
     CDS             24971..25777
                     /locus_tag="VYR08_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3465630.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            25774..26547
                     /locus_tag="VYR08_000031"
     CDS             25774..26547
                     /locus_tag="VYR08_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3465631.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            26544..27614
                     /locus_tag="VYR08_000032"
     CDS             26544..27614
                     /locus_tag="VYR08_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3465632.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEVPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            27875..28114
                     /locus_tag="VYR08_000033"
     CDS             27875..28114
                     /locus_tag="VYR08_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465633.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            28389..28874
                     /locus_tag="VYR08_000034"
     CDS             28389..28874
                     /locus_tag="VYR08_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV3465634.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            28896..31514
                     /gene="alaS"
                     /locus_tag="VYR08_000035"
     CDS             28896..31514
                     /gene="alaS"
                     /locus_tag="VYR08_000035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV3465635.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGRRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31604..33058)
                     /locus_tag="VYR08_000036"
     CDS             complement(31604..33058)
                     /locus_tag="VYR08_000036"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV3465636.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            33237..33986
                     /locus_tag="VYR08_000037"
     CDS             33237..33986
                     /locus_tag="VYR08_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465637.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            33967..35601
                     /locus_tag="VYR08_000038"
     CDS             33967..35601
                     /locus_tag="VYR08_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465638.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILDLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            36284..37447
                     /locus_tag="VYR08_000039"
     CDS             36284..37447
                     /locus_tag="VYR08_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV3465639.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            37444..38121
                     /locus_tag="VYR08_000040"
     CDS             37444..38121
                     /locus_tag="VYR08_000040"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV3465640.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            38111..38965
                     /locus_tag="VYR08_000041"
     CDS             38111..38965
                     /locus_tag="VYR08_000041"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV3465641.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRSYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQFSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            38984..40051
                     /locus_tag="VYR08_000042"
     CDS             38984..40051
                     /locus_tag="VYR08_000042"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV3465642.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            40061..41227
                     /gene="aroC"
                     /locus_tag="VYR08_000043"
     CDS             40061..41227
                     /gene="aroC"
                     /locus_tag="VYR08_000043"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV3465643.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAARSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            41237..42340
                     /locus_tag="VYR08_000044"
     CDS             41237..42340
                     /locus_tag="VYR08_000044"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV3465644.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEYLITENTDYTVVIK"
     gene            42351..42689
                     /locus_tag="VYR08_000045"
     CDS             42351..42689
                     /locus_tag="VYR08_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV3465645.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            42781..44064
                     /gene="aroA"
                     /locus_tag="VYR08_000046"
     CDS             42781..44064
                     /gene="aroA"
                     /locus_tag="VYR08_000046"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV3465646.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            44057..44533
                     /locus_tag="VYR08_000047"
     CDS             44057..44533
                     /locus_tag="VYR08_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV3465647.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            44530..45378
                     /gene="pheA"
                     /locus_tag="VYR08_000048"
     CDS             44530..45378
                     /gene="pheA"
                     /locus_tag="VYR08_000048"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3465648.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSVEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGTYPIYPISD
                     HGKERR"
     gene            45375..46649
                     /gene="psr"
                     /locus_tag="VYR08_000049"
     CDS             45375..46649
                     /gene="psr"
                     /locus_tag="VYR08_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV3465649.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            46928..47773
                     /locus_tag="VYR08_000050"
     CDS             46928..47773
                     /locus_tag="VYR08_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3465650.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            47763..48866
                     /locus_tag="VYR08_000051"
     CDS             47763..48866
                     /locus_tag="VYR08_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3465651.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            48868..49854
                     /locus_tag="VYR08_000052"
     CDS             48868..49854
                     /locus_tag="VYR08_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3465652.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            49870..51303
                     /locus_tag="VYR08_000053"
     CDS             49870..51303
                     /locus_tag="VYR08_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV3465653.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGVVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            51332..52366
                     /locus_tag="VYR08_000054"
     CDS             51332..52366
                     /locus_tag="VYR08_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV3465654.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            52566..53303
                     /gene="mecA"
                     /locus_tag="VYR08_000055"
     CDS             52566..53303
                     /gene="mecA"
                     /locus_tag="VYR08_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV3465655.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            53454..54740
                     /locus_tag="VYR08_000056"
     CDS             53454..54740
                     /locus_tag="VYR08_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV3465656.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            54742..55611
                     /locus_tag="VYR08_000057"
     CDS             54742..55611
                     /locus_tag="VYR08_000057"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV3465657.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALHIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            55687..55764
                     /locus_tag="VYR08_000058"
                     /pseudo
     CDS             55687..55764
                     /locus_tag="VYR08_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            55906..56844
                     /gene="msrB"
                     /locus_tag="VYR08_000059"
     CDS             55906..56844
                     /gene="msrB"
                     /locus_tag="VYR08_000059"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3465658.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            56904..58628
                     /locus_tag="VYR08_000060"
     CDS             56904..58628
                     /locus_tag="VYR08_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465659.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            58630..60378
                     /locus_tag="VYR08_000061"
     CDS             58630..60378
                     /locus_tag="VYR08_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465660.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSLVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFVKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            60635..61894
                     /locus_tag="VYR08_000062"
     CDS             60635..61894
                     /locus_tag="VYR08_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV3465661.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIMDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQKNMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    61970..62105
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            62144..62644
                     /locus_tag="VYR08_000063"
     CDS             62144..62644
                     /locus_tag="VYR08_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV3465662.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            62720..63088
                     /gene="rplL"
                     /locus_tag="VYR08_000064"
     CDS             62720..63088
                     /gene="rplL"
                     /locus_tag="VYR08_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV3465663.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT        18295 a        11853 c        14355 g        18685 t
ORIGIN      
        1 ataaggaatg atgaaagatg agtgaaagtt atcaagttgg aatgtttgta tctaaatata
       61 ttagcatgta tttagataag atgtctgcaa tcctttatat atgagattga ttacgatata
      121 gttaaagtgt tagttcaata gtaaatatat cattctaagt ctattttata tataaagtat
      181 attgtttaac gtttgataga aaagatgcta tactagaagt aggatgtaat ggcaaaatct
      241 aatactaata agcgctatgc aatacgtaaa ctcggaaaga tttttggttc tgcggtaatc
      301 gggctaactt tatttttagg aggaatctga atacaagttt tgttcatgct gctgatggga
      361 ttcaatatgt cagagatgat actagagata aagaagaggg aatagagtat gatgacgctg
      421 acaatgggga tattattgta aaagtagcga ctaaacctaa ggtagtaacc aagaaaattt
      481 caagtacgcg aattcgttat gaaaaagatg aaacaaaaga ccgtagtgaa aatcctgtta
      541 caattgatgg agaggatggc tatgtaacta cgacaaggac ctacgatgtt aatccagaga
      601 ctggttatgt taccgaacag gttactgttg atagaaaaga agccacggat acagttatca
      661 aagttccagc taaaagcaag gttgaagaag ttcttgttcc atttgctact aaatatgaag
      721 cagacaatga cctttctgca ggacaggagc aagagattac tctaggaaag aatgggaaaa
      781 cagttacaac gataacttat gatgtagatg gaaagagtgg acaagtaact gagagtactt
      841 taagtcaaaa agaagactct caaacaagag ttgttaaaaa aggaaccaag ccccaagttc
      901 ttgtccaaga aattccaatc gaaacagaat atctcgatgg cccaactctt gataaaagtc
      961 aagaagtaga agaagtagga gaaattggta aattactctt actacaatct atattgtaga
     1021 tgaacgtgat ggaacaattg aagaaactac ttctcgtcaa attactaaag agatggtaaa
     1081 aagacgtata aggagaggga cgagagaacc tgaaaaagtt gttgttcctg agcaatcatc
     1141 tattccttcg tatcctgtat ctgttacatc taaccaagga acggatgtag cagtagaacc
     1201 agctaaagca gttgctccaa caacaggctg gaaacaagaa aatggtatgt ggtattttta
     1261 taatactgat ggttccatgg caacaggttg ggtacaagtt aatggttcat ggtactacct
     1321 caacagcaac ggttctatga aagtcaatca atggttccaa gttggtggta aatggtatta
     1381 tgtaaataca tcgggtgagt tagcggtcaa tacaagtata gatggctata gagtcaatga
     1441 taatggtgaa tgggtgcgtt aacagattcc tccctgcgac aaaattgata atagtatgtc
     1501 tgaatatata attatatata aaaagctata gataaggtca tcttatagcg actataaaaa
     1561 agtgaacaag cctagtcttc tgtacattag aattcagatt tgttcacttt ttgtttaata
     1621 ctcaatgaaa atcaaagagc aaactagaaa actagtcgca ggttgctcaa agtactgctt
     1681 tgaggttgta gatagaactg acgaagtcag tcacatatat aatccaaggt gacgttgacg
     1741 cggtttgaag agatttttga agagtataag tctttgattt tgagactaag gagagtcagg
     1801 gtcaaagtag tgaatgattc tgaaataact cataatagtc tcttgagagg acagataaga
     1861 gtaacaaata tggtgctact catgtgtctt gcattttgat ttacaaacag cgaagaaaat
     1921 ataggaagct gaaataaaat atagatgaat caatttggag agtcaacgca atctatggca
     1981 atattttcga agctaggggg aagcatttta atttcaatgt actaggcgtc ttctacagct
     2041 agctagagtt tcaattgtag atgttctttg aaataatatc ttagcgattt attggtatta
     2101 gaagaattag agaaattctt tctcaataaa taaacatggc gtcaccaaaa ctgaagaagc
     2161 ggtagtgttc ttggatggca tggtggtagg catctaagac taattcacgg cctgcaaagg
     2221 cagaaaccaa catgaccaga gttgattttg gcagatggaa gttggttgag aaggcatcca
     2281 cgaccttcca ctcataccca ggtttgataa agatattggt ccaaccagaa tctgcttgga
     2341 tttgcccatc aaacttggaa ccaatagttt ccaaggtgcg gatagaagtg gttccgacag
     2401 cgatgacgcg accaccattt tctttgacag agcgaagggt ggcagcagct tcctcagaaa
     2461 gttgatagaa ctctgagtgc atttcgtgtt cgtccagatt atccacagaa acaggtctaa
     2521 aggttccgag tccgacatgg agagtcagat agactagatg aacaccctta gcttggattt
     2581 ctgccagcag ttctttggtg aagtgaagac cagcagtcgg tgctgcagca gagccgcttt
     2641 ccttggcgta gacggtttga taacgttcac ggtcatctaa tttttcgtgg atataaggtg
     2701 gcagaggcat ttctcccaga ctttccaaga cttctaggaa aattccttgg tattcaaagc
     2761 ggacaatgcg tcccccgtgg gtcaattctt ctgtaacgac agcgctgagg cggccatcac
     2821 caaagctgat acgagtaccg accttgaggc gtttggcagg tttagccaga acttcccact
     2881 cgtctccact agtgttctta aggaggagaa gttccacatg acctcctgtc tccacttttt
     2941 gaccatagag gcgggcaggg agaactcggg tgtcgttcat gacaagggca tcaccaggtt
     3001 ccagcatatc aataatagag tggaagtgtt tatcttgcat ttctcctgtc tcacggttga
     3061 cgatgaggag tttggaggca tcacgttttt caaggggcgt ttgggcaatc aattcctctg
     3121 gcaagtggaa atcaaaatca gctgtattca tatatttgtt cattctttct aagttctaat
     3181 cctatccatt ataacacgtt tcaagtttgg acgctctttt ttttagaaat taaatcgttt
     3241 tcacttgaca aaaattggtc tataccatat aataaatata gacagaaatg gaggatgaaa
     3301 agatgaaagt tattaaagtt gaaaaccaag ttcaaggtgg aaaagttgct tttgagattt
     3361 tgaaggaaaa attggctaat ggagctcaaa ctctaggact tgcgacagga agtagcccac
     3421 ttgaatttta caaggaaatt gttgagagtg accttgattt ttcaaatcta accagtgtca
     3481 accttgatga gtatgtgggc cttgatgggg acaatccaca gtcttatcgt tacttcatgc
     3541 aagaaaactt gttcaaccaa aaaccattta aagaaagttt cttgcctcgt ggtgttaagg
     3601 acaatgctga agctgaagtt gaacgctaca accaaatttt ggctgaccat ccagttgacc
     3661 tccaaatctt gggaatcggt cgcaatgggc atatcggctt taatgagcct ggtactccat
     3721 ttgacagtca aacgcatcta gtagaacttg accagtctac tatcgaagcc aatgcacgct
     3781 tctttgccaa gattgaagac gtcccaaccc aagctatttc aatggggatt aaaaacattt
     3841 tggatgccaa gtctattatt ctttttgctt acggtgagtc gaaagcagaa gccattgccg
     3901 gaacagtatc tggcccagtg actgagaacc tacctgcaag tagccttcaa aatcaccctg
     3961 atgtaaccat catcgcagat gctgaagcgc ttagcttact cgaaaaataa aaaagcaatg
     4021 ttctattgaa cgctttcaac tcttgtataa atggaagaaa aaatcaaaat taaaccgcat
     4081 ttttgcttga caattattcc ttttacgtgt agaatgaaat agatattgaa cttgaaggga
     4141 gtgaaaaaaa tgtctaaaac agtagtacgt aagaatgaat ctcttgacga cgcacttcgc
     4201 cgtttcaaac gtgcggttac taaagctggt actcttcaag aaacacgcaa acgtgaattc
     4261 tatgaaaaac cttctgtaaa acgtaaacgt aaatcagaag tagctcgtaa acgtaaaaaa
     4321 ttctaattga aatgaaaggc tagagaaatc tagtcctttt tcttttaaat aaatactcca
     4381 aagcctgcaa aaatctgaaa cttcctccta caatttgata taatagagag aagaactcat
     4441 ttgaaggagg aaatgatgtc ggttttagta aaagaagtga ttgaaaagct tagactagac
     4501 attgtctatg gtgaaccaga attgcttgaa aaggaaatca atacagcgga tattacgcga
     4561 cctggtcttg aaatgacagg ctattttgac tactatacac cagagcggat tcaacttttg
     4621 gggatgaagg agtggtctta tctgatcagc atgccttcca acagccgtta tgaagttttg
     4681 aaaaaaatgt ttctacctga gacaccagca gtcattgttg cccgtggttt ggtggttcca
     4741 gaggagatgt taaaggctgc tagagaatgt aagattgcta ttttaaccag ccgtgcagct
     4801 accagtcgtt tatctggaga gttatctagc tatctggatt ctcgtttggc agaacgtacc
     4861 agtgtgcacg gtgtcttgat ggatatttat gggatgggcg tcttgattca gggagatagt
     4921 ggaattggta agagcgagac aggtcttgag cttgtcaaac gtggtcaccg tttggtagcc
     4981 gatgaccgtg tcgatatctt tgccaaggat gagattactc tctggggtga accagctgaa
     5041 attttgaaac acttgattga aattcgtggg gttggtatta tcgatgttat gagtctctac
     5101 ggtgcgagtg ctgtcaagga ttcttcacag gttcagcttg ctgtctattt ggaaaattac
     5161 gatacacata agacctttga tcgtcttgga aacaatgcag aggaacttga agtttctggc
     5221 gtagccattc ctcgtattcg tattccagtt aaaacaggtc gtaatatctc tgttgtgatt
     5281 gaggcagctg ccatgaatta tcgtgccaag gaaatgggct ttgatgcgac ccgtttgttc
     5341 gacgaacgac tgacaagtct catcgctcga aatgaggtgc aaaatgcttg atccaattgc
     5401 tattcaacta ggacccctag ccattcgttg gtatgcctta tgtattgtga caggcttgat
     5461 tcttgcggtt tatttgacca tgaaagaagc acctagaaag aagatcatac cagacgatat
     5521 tttagatttt atcttagtag cctttccctt ggctatttta ggagctcgtc tctactatgt
     5581 tattttccga tttgattact atagtcagaa tttaggagag atttttgcca tttggaatgg
     5641 tggtttggcc atttacggtg gtttgataac tggggctctt gtgctctata tctttgctga
     5701 ccgtaaactc atcaatactt gggactttct agatattgcg gcgcctagcg ttatgattgc
     5761 tcaaagtttg gggcgttggg gtaatttctt taaccaagaa gcttatggtg caacagtgga
     5821 taatctggat tatctacctg gctttatccg tgaccagatg tatattgagg ggagctaccg
     5881 tcaaccgact ttcctttatg agtctctatg gaatctgctt ggctttgcct tgattctgat
     5941 ttttagacgg aaatggaaga gtctcagacg aggtcatatc acggcctttt acttgatttg
     6001 gtatggtttc ggtcgtatgg tcatcgaagg tatgcgaaca gatagtctca tgttcttcgg
     6061 acttcgagtg tcccaatggc tgtcagttgt ccttatcggt ctcggtataa tgatcgttat
     6121 ttatcaaaat cgaaagaagg ccccttacta tattacagag gaggaaaact aaatgttaga
     6181 agttgcatat attcttgttg ccctagcttt gattgtcttt ttggtctatc tgatcattac
     6241 tgtacaaaag cttggtcgtg tcatcgatga aacagaaaag acgattaaaa ccttgacttc
     6301 agatgtggat gtgaccttgc atcacaccaa tgagttgttg gctaaggtca atgtcttggc
     6361 agatgatatc aatgtcaagg tggctacgat tgatccactc ttcagtgctg ttgcagattt
     6421 atctctatct gtttcagacc tcaatgacca tgcgcgtgtc ttgagcaaga aagcttcatc
     6481 agctggttca aaaacactca agactggtgc aagtctgtca gctcttcgtc ttgcaagtaa
     6541 atttttcaaa aaataaaaaa ggagaatcct tatgggtaaa ttatcctcaa tccttttagg
     6601 aaccgtttca ggtgcagctc ttgccttgtt tttaacaagt gataagggca aacaagtttg
     6661 cagtcaggct caagattttc tagatgattt gagagaagat ccggagtatg ccaaggagca
     6721 agtctgtgaa aaactgacag aagttaagga gcaggctaca gattttgttc tgaaaacaaa
     6781 agaacaggtt gagtcaggtg aaatcactgt ggacagtata cttgctcaag ctaaatccta
     6841 tgcttttcaa gcgacagaag catcaaaaaa tcaattaaat aatctcaagg aacaatggca
     6901 agaaaaagcc gaagctcttg atgactcaga agagattgtg attgatataa cagaagaata
     6961 aaccatcacc atctccgaac ggactatgta tctggggatg gtgattttta tctggaatct
     7021 agtctttgtg gtataataat tactatgcag aaaaaaacaa cgtcagccta tgtgcacatc
     7081 ccattttgta cccagatttg ttattattgt gatttttcaa aggtcttcat caaaaatcag
     7141 ccagtcgaca gctatttaga gcatctgctg gaagagtttc gttcttatga tattgaaaag
     7201 ttgtcaaccc tttatatcgg tggtggaaca ccgacagccc tgtcggctcc gcaactggag
     7261 gttttactga atggcttgac taaaaacttg gacttgtctg tcttagaaga gttgaccatt
     7321 gaagccaatc caggcgattt ggatgcggat aagatagctg ttttgaaaaa ttcggctgtc
     7381 aatcgtgttt cgctaggtgt ccagaccttt gatgataaga tgttgaaaaa gattgggcgc
     7441 agtcatttgg agaaggatat ttatgaaaat atcgatcgcc tgaaactggc tggttttgac
     7501 aacatctcca ttgatttgat ttatgctctg cctggtcaga ccatggagca agtaaaggaa
     7561 aatgtggcta aagccattgg actggatatt ccccacatga gtttgtatag tttgatttta
     7621 gaaaaccata cggtctttat gaaccggatg cgacgtggga aattgcctct gcctaaggag
     7681 gaactagaag cggagatgtt tgagtacatc attgcagagc tggagcgagc gggttttgag
     7741 cattatgaga tttctaattt ctccaaaccc ggctttgaaa gtcgtcacaa tctcatgtac
     7801 tgggacaatg ctgaatacta tggtattggt gcaggggcat ctggttatgt caatggagta
     7861 cgctataaaa atcatggtcc cattcgtcat tatctcagtg cggttgagga aggcaatgct
     7921 tgtattacag aagatcacct gagtcaaaag gagcaaatgg aagaagaaat gttcttggga
     7981 ctccgcaaga aatccggggt ttccatggcg cgatttgagg aaaaatttgg acagtctttt
     8041 gctggacttt atggagaaat tgtcagagat ttggttcaac aaggcctcat gcagattgag
     8101 ggtgaccacg ttcgcatgac aaagagaggt ctctttttgg gagatactgt agcagaacga
     8161 tttattttgg agtaggatca tgggcttaac ttatcaaatg aaaatgaaaa ttccttttga
     8221 tatggctgat atgaacggtc atatcaaact tccagatgtg attttgctgt cactgcaagt
     8281 ttcagggatg cagtcgattg aactgggagt tagtgataag gccattttgg aagactataa
     8341 tctggtctgg attatcgcag aatatgatat tgaggtggtt cgtttgcctc gttttgcgga
     8401 agaaattacc atcgaaacgg aagccttgag ctacaatcga cttttttgct accgttgctt
     8461 tactatttat aatgaagcgg ggcaggagct catccatatg atggcgacct ttgttctcat
     8521 ggaccgcgac agtcgaaaag tccatgctgt tgaacctgag attgtggctc cttaccagtc
     8581 tgattttgat aaaaaactta tccgtggacc gaagtatgag tccttggaag agctgatcag
     8641 caaggattac catgttcgtt tttacgactt ggatatgaat ggtcatgtca ataacagtaa
     8701 atacttggac tggatttttg aggtcatggg agcggatttt ttgacccact atattcccaa
     8761 gaaaatcaac ctcaagtatg tcaaggaagt tcgaccaggt ggggtgatta catcggctgt
     8821 tgaacggact ggactggaaa gcaagcatga aattacaagt gatggggtta tcaatgccca
     8881 agcaatcatt acttggcaag aaataaaaaa ggcttaggga gaaatagatg aaatataaag
     8941 gctatttaat tgatttagac ggaaccattt ataaggggaa agaccgaatc cctgctggag
     9001 agacttttgt tcatgaattg caaaagcggg gcattcccta tctctttgta accaacaata
     9061 caactcgcac tccagagagt gttaaggaga tgttggctca gaattttaat atcgatacgc
     9121 ccctatcgac tgtctacaca gcgactttgg caaccatcga ctatatgaat gatttggggc
     9181 ttgaaaagac cgtctatgtc gtcggagaag caggactcaa ggaagtcatc aaggcggctg
     9241 gttatgtgga agacaaggaa aaccctgcct acgtggtagt tggtctggat tggcaagtcg
     9301 actatgaaaa atttgccaca gcaactcttg ctattcaaaa gggtgcccat tttatcggaa
     9361 ccaatcctga cctcaacatc ccgacggaac gtggtctttt gccaggtgct ggctcactga
     9421 ttacgctgct tgaagtagcg acacgagtga agccggttta tatcggaaaa ccaaatgcca
     9481 tcattatgga caaggcggtt gagcacttag gtttggaacg tgaagagtta atcatggttg
     9541 gggacaatta tttgactgat atccgggctg ggattgacaa tggcattcca acactcttgg
     9601 tgacgacagg ttttaccaag gcagaagaag tagctggcct gccaatcgca ccgacacatg
     9661 tggtttctag ccttgcggag tgggattttg atgaaaacta aattgacctt ttggggctct
     9721 atgctctttc tcctctccct ctcaatcctt ctgaccattt atctggcttg gattttctat
     9781 cctatggaga ttcagtggct aaacttaacg aatcgagtct atctaaaacc agaaaccatt
     9841 caatacaatt ttcatatctt gatgaattat ctgaccaatc cttttagtca ggtcttacag
     9901 atgcctgatt ttcgttcgtc agcagctggt ctgcaccatt tcgcagtggt caagaatctc
     9961 tttcatttgg ttcagctagt agctctagtg acactgccaa gtttctatgt ctttgtcaat
    10021 aggattgtga aaaaggactt tttgtctctt tatcgaaaaa gtctcctgac tctagtagtc
    10081 ttacctgtga tgattggact tggggggggg gtgattggtt ttgaccaatt ctttactctt
    10141 ttccatcaaa ttctctttgt gggagatgat acatggcttt ttgatccagc caaggatcct
    10201 gttattatga ttttgccaga gaccttcttt cttcatgcct tcctcctctt ttttgccctc
    10261 tatgaaagct tctttgggta tctgtgtctg aaaagtcgta ggaagtgaaa tgaaagatgt
    10321 ttttaatttg catatataaa aatatatatc gtttaaaacc atcgttaaga gtgaattaac
    10381 caaatccaat tgtgtcaatc gttcgcaaac agcctatttt tcttgaaaaa ataggctttt
    10441 tttctaaaaa tccctagtca agcgccttat ttttttgtat aatagaagta gcaaagtata
    10501 gataagaaga gaaaagcatg attacactat ttctatcacc gagctgtaca tcatgtcgta
    10561 aggctaaggc ctggttagaa aaacataagg ttccctttgt ggaacacaat attatgacca
    10621 gtcctttaac aagaaaagaa ttgcaacaca tcctttcctt gaccgaaaat ggtactgatg
    10681 acatcatttc aactcgttca aaaatttttc aaaaattgaa tattgatgta gagagtattt
    10741 cggtatcgga attgcttcat ttgattgagc agtatcctag tcttttgcgt cgtccaatta
    10801 ttattgatgc caagcgtatg caaatcggtt ttaatgaaga tgagattcgt gcttttctcc
    10861 ctcgtagtta tcgtaagcaa gaactaaaag aagcaagaat gagagctggt attagttaat
    10921 gaacaaacag tatagttacc cactagattt gtcgtggagc actgaagaac ttgcttcagt
    10981 gctttctttt tttaatgatg ttgaagctgc ctatgaaggc aaagtagagg ctaaaaagtt
    11041 actggactcc tataagggat tcaaagcggt cgtgccaagc aagagcgaag agaaacgttt
    11101 gggacgagaa tttgaaacgg ttagtggcta ttcactttac cgagcagttc aggctgccaa
    11161 agaaaaaggg gaaggaaaga tttctcttgg aaagtaaatt tgaatttgct aaagagcttg
    11221 ttaagaaagc aggccagtac attcttgacc atatgcagga agacttgcgt gttgaaacca
    11281 agtcctctcc aacagatttg gtgaccagac tggacaaaga agttcaggaa ctcttggttg
    11341 gtgagatttt gtcccgttat cctgaggata agatttgtgc tgaagaaggt tgtctgcgag
    11401 cctcggttca agagggcaag gtttgggtca ttgatcccat tgatggtacc aataattttg
    11461 tagcccagca ggaagatttt gctgttatga tggcttattt tgaaaatggt cagggacagt
    11521 ttggtctgat ttatgatgtg gtcaaagggg attgttacca cggtggtggg gaatttccag
    11581 tttgtctaaa tgataggccc ctagctcctt ttaaaactaa accacttgga gattttctca
    11641 ttgcagggaa tagtggtatg ctggaaacca atgaatgggg gctggctgat ttgagccgat
    11701 cggttcttgg tgttcgtgtc tatggaagtg cggccattag ttttgccaag attttgtcag
    11761 ggcgtttgtt gacctatctc acttatctgc aaccatggga ttatgccgca gctagtattt
    11821 taggggaaag tctgggctat cgggttgtga ccctttttgg tgaagctcct gattttcaaa
    11881 ccagacagcc ggtcatgatg gtgcctcttg agatgcagga ggaaattcag tcctatattt
    11941 acgaaaggaa aagaacttaa atgcaatttc cagaaggatt tgttgaaaaa tataaagaga
    12001 tactaggaga tgaggcaaga gattttcttg cctcttttga ggaggaagcg gtttcggcct
    12061 ttcgggtcaa tcccttaaaa gaagagcaac tttccttttc tgatgccatt actcaaaccc
    12121 cttggggcca ctatgggaag gtttcaggga aatcgcctga gcatgctaca ggtttagttt
    12181 attcgcaaga acctgctgct caaatggtgg ctcaagtagc ccaacccagt cctggtatga
    12241 aggtcttgga cttggctgct gcaccggggg gcaaatcaac tcaactggca gcctatctag
    12301 caggggaggg gctacttgtt tccaatgaaa tttcaagcaa acgggctaag attttggttg
    12361 aaaacatgga gcgttttgga gcgacaaatg tcgtggtgac caatgaatct gccgactgct
    12421 tggtcaaggt ctttaaaggc tattttgact taatcgttct tgatgcccct tgctctggtg
    12481 aaggaatgtt tcgtaagcaa cctgatgcta tggactattg gagcttagat tatccgagtc
    12541 aatgtgctag cttgcaaaga gaaattctgg aggatgcagt gaccatgtta gctgaaggtg
    12601 gtcgtctggt ttattcgacc tgtacctggg cacccgagga aaacgaagag attgtcaatt
    12661 ggttgctgga agagtatgat tttgacttgt tgccagtaga gcatatcaat ggaatggtag
    12721 ctggtattga cctgccagaa acggctcgga tgtaccctca tcagtttaag ggagagggtc
    12781 agtttgttgc ccatctacag tttaagggta acaatccagc tcctaaattt aaggcaagta
    12841 agagtaacct cagccgtgaa caagttgcct tgtggcagga atttgcccaa aaccatttga
    12901 aggtcaatct accgggtatc ttgcagactt ttggagacca gctttatctc ttgccagaac
    12961 ttttgccaga tttagggaag ctcaagattg ctcgcaatgg actgcatctg ggcactttta
    13021 agaagaaacg ctttgagccc agtttcgctc ttggtttagc cttgaaaccg agtcaggtcg
    13081 aacagtcggt tgaaattggc caagaagcgt ttgtcaagta tgcggctgga gaaatcgttc
    13141 agctggcaga aagtctgcca aatggttggt accaagtttt ggttaaaggc aatggcttgg
    13201 gctttgcaaa ggttactgga aatgttttga aaaattattt tccaaaaggc ctcagattca
    13261 aatgaaaatg ctagtcaaat atcttattct atgttatagt gaaagtatgg atttttttag
    13321 taggatgatt cttgatatct tgattgccat tacatgtagt tttactactc aatgaaaatc
    13381 aaagagcaaa ctaggaagct agccatagat agctcaaaac actggtttga agagattttt
    13441 aaagagtaca agaagtttag ttattagcgt tcttaccgct tgtaaactag atttctcata
    13501 aaatagaatc ttttcctttt agttgtaaac tagtctggga gagtagagag gtttgagata
    13561 cctttctagc ttttggatta tcatctaagg agagtaattt cccttgcatt aaaaagggga
    13621 aaaagagaca cgaaatgact ataatgggtg acaatggggg aagggataga caagagattt
    13681 tatccacata tgaaaaaagg aggttaggaa agagtaatat agcctatatt atataaataa
    13741 tcaattgcgc agaaatttgg taagaattca tgcgtcaact cataaagaac tacttaaaaa
    13801 attcacagta ttcataatta ttttcgagga gaaaaacagt gaaaaaaaga aaaaagcttg
    13861 ctctgtctct tatcgctttt tggctgacgg cttgtttagt aggctgtgct agctggattg
    13921 atcgtggaga atccataacg gctgttggct caactgcctt gcaacccttg gttgaagtag
    13981 cggcagatga atttggcacg atccatgttg gaaaaacggt caatgtccaa gggggaggtt
    14041 ctggtacagg cttgtcccag gttcagtctg gggcagttga tataggaaac tcagatgtat
    14101 ttgctgagga aaaagacgga attgatgctt ctgctcttgt tgatcacaag gtcgcggtag
    14161 ctggcttggc tctgattgtc aataaggagg ttgatgttga taacctaacg acagagcaac
    14221 ttcgtcaaat cttcataggt gaggtaacca attggaaaga ggttggtggt aaggacttac
    14281 ccatctctgt tatcaatcgg gcagccggct ctggctctcg tgctaccttt gatactgtca
    14341 ttatggaagg tcagtctgcc atgcaaagtc aggagcagga ttcaaatgga gcggtaaaat
    14401 caatcgtatc aaaaagtcca ggagctatct cttatttatc tcttacctat atagatgatt
    14461 cggtcaaaag catgaagttg aatggctatg acttaagtcc agaaaatata agtagcaata
    14521 attggccctt gtggtcttat gagcatatgt atacattggg gcagcccaat gagttggctg
    14581 cagaatttct caattttgtt ctctcggatg agacccaaga agggattgtc aaacgattga
    14641 agtatattcc gattaaggaa atgaaggttg aaaaagatgc tgccggaact gtgacagtgt
    14701 tggaagggag acaataatga atcaagaaga attagctaag aaaatgttgc ttccatcaaa
    14761 gaattctcgt ctggagaaat taggaaaagg tttgaccttt gcctgtcttt ctttgatagt
    14821 catccttgtg gccatgattt tggttttcgt agcgcaaaaa ggcttgtcga ccttctttgt
    14881 caatggtgtg aatatctttg actttctttt gggaggaact tggaatcctt ctagtaaaga
    14941 atttggtgcc cttcctatga ttttgggttc ctttatcgtt accattctct cagcccttat
    15001 cgcaacaccc tttgctattg gtgcagcagt ttttatgacc gaagtatcac caaaaggggc
    15061 gaagatttta caaccagcta ttgaactcct ggttgggatt ccttcagtag tgtacggatt
    15121 tattggcttg caagtcgtcg ttccctttgt tcgcagtgtc tttggtggga ctggttttgg
    15181 gattttgtca gggatttccg tcctctttgt catgattttg ccgaccgtaa cctttatgac
    15241 aacggatagc ttgcgtgcgg ttcctcgtta ttatcgtgag gccagtttcg ctatgggagc
    15301 cactcgctgg cagactatct ggcgtgtaat cttgaaggcg gcccgttctg gtattttcac
    15361 tgcagtggtc tttgggatgg cgcgtgcctt tggtgaggct ttagctatcc agatggttgt
    15421 cggaaactca gctgttatcc caacttcctt gaccacacca gctgcaactt taacttctat
    15481 attaactatg ggaattggga acactgtcat gggaactgta aataataatg ttctctggtc
    15541 actggccttg gtactgctct tgatgagttt agtttttaac agtgtgatta aattgattac
    15601 gaaagaaaga ggaaagaaaa attatgcgcg ctaagaaatt agataaactt gcaacagctg
    15661 tcctctatac gattgctagc atcattgtga caatcttggc ttccttgatt ctctatatct
    15721 tggttcgggg cttgccccat atctcttggt ctttcttgac tggaaggtct tctgcttttc
    15781 aagcaggtgg tgggattggc attcagcttt acaattcctt tttcctattg gtcattacct
    15841 tgattatttc tgtacctctt tctatgggag ctgggattta cttggctgaa tatgctaaaa
    15901 aaggtcctgt taccaacttt gtgcggactt gtattgaaat cttgtcctct ttaccatcag
    15961 tggtggtggg tctctttggt tacttgatct ttgtagtcca gtttgagtat ggattttcaa
    16021 tcatttcagg tgccttggcc ttgacagtct ttaacttgcc tcagatgacg cgtaatgtag
    16081 aagatagttt gaaacacgtt caccataccc aacgtgaggc tggtctggct cttgggattt
    16141 ctcgctggga gacagtgatt catgttgtta ttccagaagc gcttccaggt attgtaacgg
    16201 gtgtcgtctt ggcatctggt cgtatctttg gcgaagctgc agctctgatc tatacagcag
    16261 ggcaatcggc gccagctctt gactggtcta actggaatat cctcagtgtg actagcccca
    16321 tctctatctt ccgtcaagca gaaaccttgg ctgtccatat ctggaaagtc aatagtgaag
    16381 gcactattcc agatggaacc attgtatcag caggttctgc cgctgtgctc ctgatcttta
    16441 tcctgatttt taactttgga gctcgtaagt tcggaagcta tctacacaag aaattaaccg
    16501 ctgcctaaag gagaagccat gtcaacatat aactgggatg agaagcatat ccttaccttt
    16561 cctgaagaaa aagtagccct ttctactaag gatgtccatg tttactatgg taaaaatgaa
    16621 tccattaagg ggattgatat gcaatttgaa agaaataaaa ttacagcttt gattggtccg
    16681 tcgggatcgg ggaaatctac ctacttacgc agtctcaatc gcatgaatga tactattgat
    16741 attgctaaag taactgggca gattctctat cgtggaattg atgtcaaccg tccagaaatc
    16801 aacgtttatg aaatgcgtaa acacattgga atggtttttc aacgccccaa tccatttgct
    16861 aagtcaattt accgtaatat tacctttgcg catgaacgtg ctggagttaa ggataagcaa
    16921 gtcctagatg aaatcgtaga aacctccctt cgtcaggctg ccctctggga tcaggttaaa
    16981 gacgatctcc acaagtcagc cttgacctta tcaggtggtc agcaacaacg tctctgtatc
    17041 gctcgtgcca tctctgttaa gccagatatc ctcttaatgg atgagccagc ctcagccttg
    17101 gatccgattg cgaccatgca actagaagag accatgtttg agctcaagaa aaactttacc
    17161 atcatcattg taacgcataa tatgcagcag gctgctcgtg caagtgacta tacaggcttc
    17221 ttttacttgg gtgatttgat tgagtatgac aagactgcaa ctattttcca aaatgccaag
    17281 ctacagtcca ccaatgacta tgtatctggt cactttggtt agaaaggaaa ccgtatgaca
    17341 gatgcgattt tacaggtatc agacctgtcc gtttattata ataaaaagaa ggctttgaat
    17401 agtgtttccc tatctttcca acctaaggaa attacagcct tgattggtcc atctggatca
    17461 gggaagtcaa ccctcctcaa gtctctcaac cgcatgggag atctcaatcc agaggtgacc
    17521 acaactggat ccgtggtgta caatggtcac aacatctaca gtccgcgtac agatacggtt
    17581 gaattacgta aggaaatcgg aatggttttc caacaaccta atcctttccc tatgactatc
    17641 tatgagaatg ttgtctacgg gcttcgtatc aatggaatta aggataagca ggttctggat
    17701 gaagccgtag aaaaagcctt gcaaggtgcc tctatctggg atgaggtcaa ggatcgtcta
    17761 tatgattcag ctattggatt gtcaggtggt caacagcagc gtgtctgcgt ggcccgtgtc
    17821 ttggcaacta gtcctaaaat catcctcttg gatgagccaa cttcggcttt ggatccgatt
    17881 tcagctggta aaattgagga aaccttgtat ggtctaaaag acaagtacac catgcttctg
    17941 gtaacccgtt ccatgcagca agcttcacgt atctctgata agacaggatt tttcctagat
    18001 ggagatttga ttgaatttaa tgataccaag cagatgttcc ttgatcccca acacaaggaa
    18061 acggaagact atattacagg aaaatttgga taaggagatg aaagatgtta cgatctcaat
    18121 ttgaagaaga tttagagaaa ttacataacc agttctacgc tatgggacaa gaagtgctct
    18181 cacaaatcaa tcgtacggta cgtgcttttg tcacgcatga ccgtgacctg gcaaaagagg
    18241 tcatcgaaga tgatgcagaa gtaaatgaat acgaagtgaa actggaaaag aaatcatttg
    18301 aaatgatcgc actccaacaa ccagtctctc aagatttgcg tacagtcttg actgtcctta
    18361 aggctgtatc agatgtggag cgtatggggg atcacgctgt agccattgcc caggcaacca
    18421 tccgtatgaa gggggaagaa cgtattccag ctgtagagga agaaattaaa aaaatgggac
    18481 gtgaagttaa aagcgttgtt gaagcagcac ttgatcttta tcttaatggt tctgttgacg
    18541 acgcataccg ggtggcctcc atggatgagc aaattaacca ctattttgaa actatccgtg
    18601 accttgcgac tgaagagatt aagaaaaatc cagaagccat tgttacgggt cgtgattatt
    18661 tccaagttat ttcctacttg gagcgtatcg gtgactatgc taaaaatatc tgtgaatggg
    18721 ttgtctactt tgaaacaggt aagattgtcg aactataaaa tagtttaatt ctgtataaaa
    18781 aggagcttga aatcaactga tggctccttt ttttgaacga aaaaatattt ttgagataaa
    18841 aatttaaaaa acacttgaca agtaacttgg atatcgttat aattatacaa aattaacaga
    18901 gaggttgttt atttatgaaa taaaaaaaat ggatatttgt tttatgtaat tttcttgcaa
    18961 gtttcttctt agtagcttgc cagtcaggtt ctaatggttc tcagtcagct gttgaggcca
    19021 ttaagcaaaa ggggaaatta gttgtggcaa ccagtcctga ctatgcaccc tttgaatttc
    19081 aatcattggt tgatggaaag aaccaggtag tcggtgcaga catcgacatg gctcaggcta
    19141 tcgctgatga acttggggtt aagttggaaa tctcaagcat gagttttgac aatgttttga
    19201 ccagtcttca aactggtaag gctgacctag cagttgcagg aattagtgct actgacgaga
    19261 gaaaagaagt ctttgatttt tcaatcccat actatgaaaa caagattagt ttcttggttc
    19321 gtaaggctga tgtggaaaaa tacaaggatt taactagcct agaaagtgct aatattgcag
    19381 cccaaaaagg gactgttcca gaatcaatgg tcaaggaaca attgccaaaa gctcaattaa
    19441 cttccctaac taatatgggt gaagcagtca atgaattgca ggctggaaaa gtagatgctg
    19501 ttcatatgga tgagcctgtt gcacttagtt atgctgctaa aaacgctggc ttagctgtcg
    19561 caactgtcag cttgaagatg aaggacggcg acgccaatgc cgttgctctt agaaaaaata
    19621 gtgatgattt gaaagaagtg gtagacaagg tcatccaaaa acttaaggat gaaggaattt
    19681 accaaagcta tcttgaaaaa gcagcaagtc taactgaagt tgaagaataa gaaaaagcag
    19741 agttggatgt cattccaatt ctgcttttaa atagtttaaa acattttcca gattgtctag
    19801 ggacaccttg gcaaattgat aaggacaggt acttaaatag gcctcttcaa agaagtccaa
    19861 gcccggttga ctgtgtttga gactggcgat tttaggatac tgtctcaggt ctagcaataa
    19921 accatcgtgt aggggataat gaggaagggg acaaggtgtt ttagttatcc tttcctcgat
    19981 ttgtttttgg taagattcat gattggtcaa gcgagccaaa cgattttttt caaacaattg
    20041 actgtcaata tagagtgaca gggccttttg cataatgagg ttgctgtcgt agtctaggat
    20101 atttttgtag gccacaaatg cttctttgat agcatttgga agaatgagtg ctgtaatacg
    20161 aagggcagga aaaaggctgg tcgagaagga cttgatataa atgacatgct cctctgtatc
    20221 aagatagtgg aaggtttggc ccttcttgga gtccaaatca cccagataat cgtcttctac
    20281 gatatagaca tcatacttgg cagctaagtt aagaatggat cgtttgtctt gctcagaata
    20341 ggaatgtccc agaggatagt gaaatcgggg aatggtgtag aaaaacttaa tttttcctgt
    20401 tttgaagtgg ccttccagct cctccaagtc aatcccatca atgcctcgtt caatcgtttg
    20461 atagtccagc ccctgtgcaa tcaagaggcg attcatccga tggtaggttg gctgttccac
    20521 caagatttcc ttggcttgtc taggaaagga tatttgagag aggataaata aggcttgttg
    20581 ggttccagaa gtcagtacta gttggttagc cttgcagtag agagcttgct caaagaggag
    20641 tttgtgaatg gactgtctta ggtcttctaa tccttcttga ttgtcatagt agttgaagag
    20701 gtagttttct cgtccaatca aggtttcatt gacacagagt cggaaatcgt cataggcact
    20761 ggcatgttcg tcggtaacct cgatttctag gtcttgatgt tgcccttgtt ctaggacata
    20821 gtagccgctc tggggcttgg catagaggta ttgttcgtgc cgtaattcca gcagggctcg
    20881 ttgaatggtg tccttgctgc agtgaaagtc aaggctcagt tgacggatag aaggcaggcg
    20941 actacccgtc ggaaatcgtt cagactcgat accatttttc agataggaaa cgacctcttt
    21001 gtacttgctt tgtttcttca ttccagacct cccttgattt tgttacattg tacccttttt
    21061 tttccttctt ggcaatgtct agagcgtttc tctcgttcac atctaggggc aaatgtggta
    21121 tacttaggag taagatttat agaataacag acaagaaagt gaatggataa gaacgtgcag
    21181 gaaccagtga aattatttca atacaatact cttggagcct tgatggctgg cctttatggt
    21241 ggtactatga cagtaggaga attgctagaa catggtgacc ttggtttggg aaccttggat
    21301 tccattgatg gggaattgat tgtcttggat ggcaaggctt atcaggccaa agggtcagga
    21361 gaccagccag aaattgtgga agtgtcacca gatgccctta ttccttacgc tgcagtggta
    21421 ccgcatcagg cagaggtcat tttccgccag cgctttgaga tgacagacaa ggaattgaaa
    21481 gaacgaattg agtcttatta tgatggggaa aatcttttcc gctctatcaa gattcggggg
    21541 gaattttcac atatgcatgt gcgtatgatt cctaagtcaa cacccgatac caagtttgct
    21601 gatgtcgcaa cccatcaacc ggaatatagt cgtgacaatg ttgcggggac tattgttggt
    21661 ttctggacgc ctgagatttt ccatggggtc agtgtggcag gctaccatct gcacttcata
    21721 tcagatgatt tgaccttcgg tggacatgtc atggattttg tcatcaagga aggcattatc
    21781 gaggtgggag cagttgacca gttggaccaa cgtttcccag tccaagaccg tcaatacttg
    21841 tttgctaagt tcaatgttga cgagatgaaa aaagatattg aaaaggcaga ataggagaag
    21901 aaaatgacca ttcatatcat tattaccatg ttgctgttgc tagcttttct gataggaagc
    21961 atttggtttg ccaaaaagaa ataccagatt aatctagctg tcttgggctt gggtgcagtc
    22021 gctttctttg tctcttcgca gattttggaa aaactggtgc atatcttgat tttgcaccct
    22081 caaaaagacg gtagtattgc cctcttgcaa gatcatccgc ttatctatat tatctatggc
    22141 ctagccatgg cagcattttt tgaggaaacg gctcgtcttg ttttcttcaa atggttggag
    22201 aaaaagagaa gtttggaaaa ggcagacgcc ttggcctatg gactggggca tggtggcttg
    22261 gagttgattt ttctaggcct tactagttta ctcaatcttt acattgttct ttcggcagtt
    22321 caaactcaga atccacaggt tatgcaatta ctgtctgaaa atatgttaaa aaccattcag
    22381 tcactttcgg tctggcagat ttatttgctt ggttttgagc gaatcttggc gcttggtttc
    22441 caattacttt tgacagtttg ggtttaccaa gctgttcgcc agaagaaatg gatttatctc
    22501 ctagcagcct atggcttgca tgccttcttt gatctggcac catctctttt ccaagtaggc
    22561 tggttgacaa atccagtctt ggttgaagtg attctagcac tggagctcgt tctggttgcc
    22621 tatggaacca aggaaatctt ttgtaaaaaa tcataaaaag gggggaacct ctttttatta
    22681 tgcaaaatcc aaacaaggta tttttatggt cgtcaaatgt ctctaaaaat ggtataatgg
    22741 aatgaatttt gtaaaaggaa gagtgacatg tctgtaagag aaaaaatgct tgaaatctta
    22801 gaaggaattg atatccgttt taaggaaccc ttgcatagct atagttatac aaaagtaggt
    22861 ggagaggctg attatttggt ctttccacga aatcgttttg agttggcgcg ccttgtgaaa
    22921 tttgccaacc aagaaaatat cccttggatg gttcttggca atgcaagcaa tatcatcgtt
    22981 cgtgatggtg ggattcgtgg atttgtcatc ttgtgtgaca agctcaataa cgtttctgtt
    23041 gatggctata ccattgaagc agaagctggg gctaacttga ttgaaacaac tcgcattgcc
    23101 ctccgtcata gtttgactgg ctttgagttt gcttgtggta ttccaggaag cgttggcggt
    23161 gctgtcttta tgaatgcggg tgcctatggt ggcgagattg ctcacatctt gcagtcttgt
    23221 aaggtcttga ccaaggatgg agaaatcgaa accctgtctg ctaaagactt ggcttttggt
    23281 taccgccatt cagctattca ggagtctggt gcagttgtct tgtcagttaa atttgcccta
    23341 gccccaggaa cccatcaggt tatcaagcag gaaatggacc gcttgacgca cctacgtgaa
    23401 ctcaagcaac ctttggaata cccatcttgt ggctcggtct ttaagcgtcc agtcgggcat
    23461 tttgcaggtc agttaatttc agaagctggc ttgaaaggct atcgtatcgg tggcgtagaa
    23521 gtgtcagaaa agcatgcagg atttatgatc aatgtcgcag atggaacggc caaagactac
    23581 gaggacttga tccaatcggt tatcgaaaaa gtcaaggaac actcaggtat tacgcttgaa
    23641 agagaagtcc ggatcttggg tgaaagcaag taggaagctg tatttgaaaa gctgctgcta
    23701 tgtcatggaa agggggtgaa agcctatcgg tagcgaagat gtatgcaggt ggttttactc
    23761 cctgcaagag gtagtgggga cctgacagag ccccgatcgg ttaatctatg aaaaagaagg
    23821 aatttatgcc aattgaaaaa accaattatc gaattcaaaa acgtctctaa agtttttgaa
    23881 gacagcaaca ccaaggttct caaagacatc aactttgagt tggaagaagg gaaattctac
    23941 acccttctag gagcatctgg ttcggggaaa tcaactatcc taaacattat tgcaggttta
    24001 ctggatgcga cgacaggaga tatcatgcta gacggtgttc gtatcaatga tattccaacc
    24061 aacaagcgcg acgtacatac cgtcttccaa tcctatgcct tgttcccaca tatgaatgtg
    24121 tttgaaaatg ttgcctttcc acttcgcttg cgtaaaattg ataagaaaga aatcgagcag
    24181 cgtgtagcgg aagttctcaa gatggttcag ttggaaggtt atgaaaaacg ttccatccgc
    24241 aaactttctg gaggacaacg tcagcgtgtt gccatcgccc gtgctatcat caaccaaccc
    24301 cgtgtggtct tgttggacga gcctttatca gcgctggact tgaaattgag aacagacatg
    24361 cagtacgaat tgcgtgaatt acaacaacga ttgggcatta cctttgtctt tgtcactcac
    24421 gatcaggaag aagctcttgc catgagtgac tggattttcg ttatgaatga tggcgagatt
    24481 gtccagtctg gaacccctgt ggacatctac gatgagccaa tcaaccactt tgttgccacc
    24541 tttatcgggg agtcaaacat cttgccaggt accatgattg aggactactt ggtcgaattt
    24601 aacggcaaac gctttgaagc ggttgatggt gggatgaagc caaatgaacc tgttgaggtc
    24661 gttattcgtc cagaggactt gcgcattacc cttcctgaag aaggcaagct ccaagttaag
    24721 gtcgataccc agcttttccg tggagttcat tatgaaatta tcgcctatga cgaacttgga
    24781 aatgaatgga tgatccactc aacccgtaag gctatcgtgg gtgaggaaat cggtctggac
    24841 tttgaaccag aagacatcca catcatgcgt ctcaatgaaa ccgaagaaga gttcgatgct
    24901 cgtattgagg agtacgtaga aatcgaagag caagaagcag gtttgatcaa tgcaatcgag
    24961 gaggaaagag atgaagaaaa caagctctaa actctttgta gtaccctaca tgctttggat
    25021 tgcgctcttt gtattggcac ccttggtctt gattttcggt caatcctttt tcaacatcga
    25081 aggccagttt agtttagaaa attataaatc ttactttgcg tcacaaaact tgacctacct
    25141 caaaatgagt ttcaactcag tgctttatgc agggattgtg acctttgtga ccttgcttat
    25201 cagctatcca acagccctct ttttgacccg tctcaagcac cgtcaactct ggctcatgct
    25261 gattatcctt cctacctgga tcaatttgct ccttaaagcc tatgctttta tcgggatttt
    25321 tggtcaaaat ggctctatta accaattctt ggaatttatc ggaattggtt cacaacagtt
    25381 gctttttacc gatttttcct ttatctttgt cgcaagctac atcgagctcc cctttatgat
    25441 tttgccgatt ttcaatgtct tagacgacat ggataataat ctcatcaatg ctagttatga
    25501 ccttggtgca actaagtggg agaccttccg tcatgtcatc ttccctctat ctatgaacgg
    25561 tgtgcgaagt ggggttcagt cggtctttat cccaagtttg agtctcttca tgctgacccg
    25621 tttgattggt gggaaccgcg ttatcacctt ggggacggct attgagcaga attttctaac
    25681 caatgacaac tatggtatgg gttcaaccat cggtgtgatt ctcatcctga ccatgttcat
    25741 caccatgtgg gtgactaagg aaaggagaga acgatgaaaa aatttgccaa cctttatctg
    25801 ggactggtct ttctggtcct ctacctgcct atcttttact tgattggcta tgcctttaat
    25861 gctggtgatg atatgaatag ctttacaggt tttagctgga ctcactttga aaccatgttt
    25921 ggagatggga gactcatgct gattttggct cagacatttt tcttggcctt cctatcagcc
    25981 ttgatagcga ccattatcgg gacttttggt gccatttaca tctaccagtc tcgtaagaaa
    26041 taccaagaag cctttctatc actcaataat atcctcatgg ttgcgcctga cgttatgatt
    26101 ggtgctagct tcttgattct ctttacccaa ctcaagtttt cacttggctt tttgaccgtt
    26161 ctatctagtc acgtggcctt ctccattcct atcgtggtct tgatggtctt gcctcgactc
    26221 aaggaaatga atggcgacat gattcatgcg gcctatgact tgggagctag tcaatttcag
    26281 atgttcaagg aaatcatgct tccttacctg actccgtcta tcattactgg ttatttcatg
    26341 gccttcacct attcgttaga tgactttgcc gtgaccttct ttgtaacagg aaatggcttt
    26401 tcaaccctat cagtcgagat ttactctcgt gctcgcaagg ggatttcctt agaaatcaat
    26461 gccctgtctg ctctagtctt tctctttagt attatcctag ttgtaggtta ttactttatc
    26521 tctcgtgaga aggaggagca agcatgaaaa aaatctattc atttttagca ggaattgcag
    26581 cgattatcct tgtcttgtgg ggaattgcga ctcatttaga tagtaaaatc aatagtcgag
    26641 atagtcaaaa attggttatc tataactggg gagactatat cgatcctgaa ctcttgactc
    26701 agtttacaga agaaacagga attcaagttc agtacgagac ttttgactcc aacgaagcca
    26761 tgtacactaa gataaagcag ggtggaacga cctacgatat tgccattcca agtgaataca
    26821 tgattaacaa gatgaaggac gaagacctct tggttccgct tgattattca aaaattgaag
    26881 gaatcgaaaa tatcggacca gagtttctca accagtcctt tgacccaggt aataaattct
    26941 ccatccctta cttctgggga accttaggaa ttgtctacaa cgaaaccatg gtagatgaag
    27001 tgcctgagca ttgggatgac ctttggaagc cggagtataa gaattctatc atgctctttg
    27061 atggggcgcg tgaggtgctg ggactaggac tcaattccct cggctacagc ctcaactcca
    27121 aggatctgca gcagttggaa gagacagtgg ataagctcta caaactgact ccaaatatca
    27181 aggctatcgt tgcggacgag atgaagggct atatgattca gaataatgtt gcaatcggcg
    27241 tgaccttctc tggtgaagcc agccaaatgt tagaaaaaaa tgaaaatcta cgttatgtgg
    27301 taccgacaga ggccagcaat ctttggtttg acaatatggt cattcccaaa acagttaaaa
    27361 accaaaactc agcttatgcc tttatcaact ttatgttgaa acctgaaaat gctctccaaa
    27421 atgcggagta tgtcggctat tcaacaccaa acctaccagc gaaggaattg ctcccagagg
    27481 aaacaaagga agataaggcc ttctatcccg atgttgaaac catgaaacac ctagaagttt
    27541 atgagaaatt tgaccataaa tggacaggga aatatagcga cctcttccta cagtttaaaa
    27601 tgtatcggaa gtaggagata aacgtaagaa gacgaatcag tcaagctggt tcgtttttaa
    27661 ggaatagaag tcagttttta gaaccctatc tttttctatt ctagttgttt atttgtttat
    27721 ttgcttattt gcttgcctag tagtatacta agcctatact cttcaaacca cgtcagcgtt
    27781 gccttgccgt actcaagtac agcttgaggc tagcttccta gtttgctttt tgatttttat
    27841 tgagtattaa ccttacagaa agcggcaaca aataatgaaa atgtctactt ttttcaaaaa
    27901 aagtttctgg ccaaccttta tgattgttaa ccaaactgct atactattcc atttaaagga
    27961 tggcttggat cgacaatacc taacaactga gtccatatat tgggtgatag gcacctttat
    28021 atttggaaat attctagtag ctgtgtttag taatatgaaa atttgggata aaaagaaaaa
    28081 tggcagtaag aaaaaatata tcctgaaaaa gtaatcagag atgtagggaa ttggtctaat
    28141 tcaacggaga tcatttccag ttgacgcaag attaaaaaaa cgatataata agaaagttga
    28201 gaattgattt tcacttgtct tagtccagag aaatggcggt gctgcgagcc atctaagcta
    28261 ggaagtcatg ctactcaatc atacaattgc ataagaacaa gagaatgaca agttcattga
    28321 atgaaggtgg taccgcggtt tttcgccctt cgtgatatga gcttgtcttt tgatttttgg
    28381 aggtgttgat gaaaacattt cttgtcaaac aaaagtttcg tcttggaggc gaacgcttcg
    28441 ctatcaagaa tgacagggga gaaattgcct atcaggtgga gggatcattt tttaagattc
    28501 ctaaaacctt taccatctat gatgcggctg gtgaacaagt cagtgagatt agtaaagaaa
    28561 tcttgacctt gcttcctcgt tttgagattc agcttcggga tggctcgagt tttgtcattc
    28621 gtaagaagtt gaccttctgg cgagataagt atgagtttga taatctaggt cttcgtatcg
    28681 agggcaatat ctgggatttg aatttcaaat tgctggatga tcgcgatcag ttgattgcgg
    28741 aaattaagaa ggaactcttc catctgactt ctacctatac cgtaacggtt cttgaagacg
    28801 cttatgcaga cctagtcatt tccctctgtg tcgcgattga ctatgtggaa atgctggaaa
    28861 gccaatcaca ttaaacaagt gaataaggag acaatatgaa acaactatct agtgcacaag
    28921 tacgccaaat gtggcttgat ttctgggcga ccaaaggtca ctcagtagaa ccatcagtaa
    28981 gtttggttcc tgtaaatgac ccaactcttt tgtggatcaa ctctggggta gcaaccctta
    29041 agaaatactt tgacgggacc attatccctg aaaatccacg tattaccaat gcccaaaagg
    29101 ctatccgtac caacgacatc gaaaacgtag ggaagaccgc gcgtcaccat accatgtttg
    29161 aaatgctggg gaacttctct atcggggatt acttccgtga cgaagctatc acttgggctt
    29221 atgagctttt gacaagccct gaatggtttg atttccctgc tgaaaaactt tacatgacct
    29281 actatccaga cgataaagat tcttacaacc gctggattga agtgggagtg gatccaagtc
    29341 acttgattcc aattgaggac aacttctggg aaatcggtgc gggaccttct ggaccagata
    29401 cagaaatctt ctttgaccgt ggggaagcct ttgacccaga aaatatcggt cttcgcctgc
    29461 ttgcagaaga tattgaaaac gaccgttata ttgaaatctg gaacatcgtt ttgtcacaat
    29521 ttaacgcaga ccctgctgtt cctcgtagcg aatacaagga attgccacat aagaacattg
    29581 atacgggcgc tggtttggag cgtttggtgg ccgttatcca aggggctaag accaactttg
    29641 aaacggacct cttcatgccg attatccgtg aagtcgagaa attgtctggt aaggtttatg
    29701 accaagatgg cgacaacatg agcttcaagg ttatcgcaga ccacattcgt tctctttcat
    29761 ttgctatcgg tgatggtgcc cttccaggaa atgaaggtcg tggttatgtt cttcgtcgtc
    29821 ttctccgtcg tgcttctatg catggtcaaa aattgggtat caacgagcct ttcctttaca
    29881 aattggttcc aactgttgga aaaatcatgg aaagctacta cccagaagtg cttgagaaac
    29941 gtgactttat tgagaaaatc gttaagagcg aagaagaatc atttgcccgt acccttcact
    30001 caggtcaaca ctttgcccaa ggcattgtag cagacttgaa agaaaaaggt caatctgtta
    30061 tcgctggttc agatgtcttc aaactctatg atacttatgg gttcccagtt gaattgactg
    30121 aagaaatcgc tgaagaagct ggtatgactg tagaccgtga aggttttgaa gcagccatga
    30181 aagaacagca agaacgcgcg cgtgcgtcag ctgtcaaggg tggctcaatg ggtatgcaaa
    30241 atgaaactct tcaaaacatc actgtagaaa gtgtcttcaa ctacaatgct agccaattgt
    30301 cttctaaatt ggtagctatc gttgctgaca atgcagaagt aggagctgtt tcagaaggaa
    30361 ctgcctctct tatctttgcg gaaacgtcat tttatgctga aatgggtgga caggtagctg
    30421 actacggaca aatcttggat gagtcaggta aggttgtggc tactgtgacc aatgttcaga
    30481 aagccccaaa tggtcaagcc cttcatacag ttgaagtcct tgcaccgctt gccttgaacc
    30541 aagaatatac cttggcaatt gatagcaatc gccgtcaccg tgtcatgaaa aaccacactg
    30601 cgactcattt gcttcacgct gcccttcaca atatccttgg aaaccacgca acacaggcag
    30661 gatctcttaa cgaagttgaa ttccttcgct ttgactttac ccacttccaa gcagtaactg
    30721 ctgaggaact tcgtgcgatt gaacagcaag ttaacgagaa aatttgggaa gcacttgaag
    30781 tgaagacagt tgaaacggat attgacactg ctaaagaaat gggagctatg gccctctttg
    30841 gtgagaaata cggcaaggaa gttcgtgtcg tgactatcgg tgactactct attgaacttt
    30901 gtggtggtac tcatgttgac aacacttctg agattggtct cttcaaaatt gtcaaagaag
    30961 aaggaatcgg ttcaggaact cgccgtatct tggcagtgac tggtaaggaa gcctttgaag
    31021 cctatcgtga acaagaggat gctcttaaag ctatcgcagc aaccttgaaa gcacctcaag
    31081 tcaaggaagt acctcacaag gtagaaggac ttcaagaaca acttcgtcaa cttcaaaaag
    31141 aaaatgctga gttgaaagaa aaagccgcag ctgcagccgc aggcgatatc ttcaaagatg
    31201 ctaaggaagt caacggtcgt cgttacattg ctagtcaagt gtctgtatcc gatgccggtg
    31261 cccttcgtac ttttgcagat aactggaaac aaaaagacta ctctgatctt cttgtcctag
    31321 ttgccgctat cggtgacaaa gtcaatgtcc ttgtagcaag caagacaaaa gaccttcatg
    31381 caggaaacct tgtcaaagaa ttagcaccaa tcatcgatgg acgtggtggt ggtaaaccag
    31441 acatggccat ggcaggagga agcaaccaag ctaagatcca agaattgttg gatgcagtag
    31501 caggtaaatt gtaatcaagt aaagatctat ccatttgggt aggtcttttt gtgaatacaa
    31561 aaaagccaaa tccgcttgga cctagcttgg ttattatgag ctattagatg gtattgactg
    31621 cccagacact tacggatcta gctgagacag ggaattgtcc ataaccttcc tcatcaattg
    31681 taacttgacc ttggtggtta ccaagtaaat ctacaaaggt ttgattagtc cattcttgac
    31741 cgacaaacat tgacttgctg ttttcttggt catttgagat aaggactgcg attggggatt
    31801 gattttcagc acctgaacgt acccaaccga tacagttagc atggtcaaag tagtcatttt
    31861 gttctccata ggccaaatct tttcggatgg ctaggaggcg gtcaaggatt tctttgaaat
    31921 cttcttgagc atactgccct gaaatcccat agtagtctcc gtaaaagaca catggaaggc
    31981 cgtcttggcg taacaaaatg agggcatagg ctgctggctt gaaccattct tcaacggtag
    32041 actcaagggc ttgtcctcgt tgggtatcgt ggttgtcgac aaaagtcaca gccttgtcag
    32101 gcttgagttc aaccaggcta tctgtgaaaa tgccacgaag gtcatagttt gcgccagctt
    32161 gactggcttc aaagagattc tggtggagac gaacatcgac aaggtcaaag tgttcttccg
    32221 ttttttcgag atagtccaga ttggcttcct tgtctgggtt ccaaaattca ccaaaaacat
    32281 agaaatcgtc accgtatttt tccttcatat cgcggatgaa gttgcgcata aagaaagagt
    32341 caatatgctt aacggcatcc aaacggaaac cagctacacc agtcgtttcc atgaaccaat
    32401 cagcccagtc atagatgttt tggatgactt caggatgttt aaagtctagg tcggcataca
    32461 tgaggtagtc gtagtttccg ttttcgttat cgaccaattc ctcgttggcc cagcccttgt
    32521 tgtccccttg gatcagataa atcccagact tacggcgttt ggcatcgtag tctgtaccgg
    32581 tgaagtggta ccaatgccag tggaagccat tataggtatc ttggcgacca tcgaaggtaa
    32641 aactagtcca gccattgatg gtgaagggtt ctccaagttc aactgtacgg tctacaggat
    32701 caacttcgat aacctgaaag gcttccctgt gatcggcagc agccttgtgg ttgagaacta
    32761 catcggccat aggttgaatt ccctgtgctt taagggcttg aatggcttga agatagtctt
    32821 ctttgaaacc atacttggtg cggacagtcc ctttttggtt gaactctcct aagtcaaata
    32881 agtcatagac cccatagccg acatcttttt cgttggttgc cttgaaggct ggtggcatcc
    32941 agacgtgact gatccccaga tgagctaggt gtggagcatt ttcagccaga cgcgtccagt
    33001 gttgaccgtc gtggggcaga taccattcaa agtattgcat gagtgtttga ttttgcataa
    33061 tgtttcctct tacttgtcaa tcttgttctt tattctatca taaagtatct ctcggcgcaa
    33121 acgtttgcgt aagatagaag gaaactattt taactactta aaataaagta ttgatttttt
    33181 tgtatcaaag tgctagtata gcgatatata atacagataa agataggagt tgtcccatga
    33241 ttgaatttca aaatgttagt aagttgtatg gtgataaaga ggccttgagt aatctcaatt
    33301 tgcagattga aaatggagag attatgggct tgattggtca taatggggct ggaaaatcga
    33361 ccactataaa atccctagtc agtatcattt cacccagcag tggtcgtatt ttggtagacg
    33421 gtcaggagtt atcggaaaat cgcttggcta ttaaacgaaa gattggctac gtagcagact
    33481 cgcctgactt atttttacgc ttaacggcca atgaattttg ggaattgatc gcctcatcct
    33541 atgatctgag tagatctgac ttggaggcta gtctagctag gctattgaac gtttttgatt
    33601 ttgctgaaaa tcgctatcag gttattgaaa ctctttctca cggaatgcgt cagaaagtct
    33661 ttgtcatcgg agcactcttg tctgatcccg atatttgggt cttggatgaa cccttgactg
    33721 gtttggatcc ccaggctgcc tttgatttga aacagatgat gaaggaacat gcacaaaaag
    33781 ggaagacagt cttgttttca actcacgtcc tagaggtggc agagcaagtc tgtgatcgga
    33841 ttgccatttt gaaaaagggg catttgattt attgtggtag tgtagaggac ttgagaaaag
    33901 attacccaga ccagtctttg gaaagtatct accttagtct tgctggtaga aaagaggagg
    33961 ttgcggatgc gtctcaaggt cattaaaaaa ttagttgata tcaatatcct ttattcatct
    34021 caagaagcta atctggctaa tctacgaaag aagcaggcta agaatcctgg gaaaaaagta
    34081 aatgtttccg ctagagtcct aagttcttac attttttcca gtctcttgat gctcatctgt
    34141 tttagtaata tagccattca ttttcctttt gaggaaatac ctatttattt tagctcgatg
    34201 gttgctattt tactagtgat tgccttttca acttctttaa cggcctttta caatgtcttt
    34261 tatgagagta aggatttggt atcgtatagg ccctatgcct ttaaagaatc agagattata
    34321 attgctaaag gactgtctgt cctcttgcca gctctaactg gaattgtacc aatcctagct
    34381 tattttctag tcctctacat taggctagct ccttctctat ggttgggttt gcctttgatg
    34441 ctactgtcct tgaccttatt atttgtctct gtggctctag tgatggtagt ggcagtgcat
    34501 ttcttggctc aaactagggt cttcagaaag tatcagtcta ttttttcgaa tgtgatgatt
    34561 gggataggag ttctcatacc tttaatattt gtcttctttc tacagtcgac ttttggaagt
    34621 attgttgaca aagttagaga cattccattt ctcctttatc ctcttcatat cttttacaaa
    34681 atagcagtgg agcctttttc aatagaagcc atattggatc tgctcgcttg gatagcttta
    34741 actgttttct tgctttacct gaccaaaaag aaggtccttc ctcgttttta tgacgtgatc
    34801 ctgcttaaca gtgaggagaa ggtcaaaaaa gaacgtcgca gcaaggagag gatttcaact
    34861 actaaaaagg gctttttccg tatggtttta cgctaccacc tcaccctctt gggacagggg
    34921 actggcgtga ttacagtgct ttttacaagt gctttccttc cttatctcat gatgatcagt
    34981 ctgatttcca aaatccgaga ttctcagata gttccagaca ttcatcctcc atactggtta
    35041 cccttgtttt ttgtaggagt gtttatagca gttgtcaata acaatatcac cagcctgcat
    35101 tcaattgcct tgtccttgga gagggaaaat gttgattttc tgaagagttt accctttgac
    35161 tttgctcgtt atgtgaaagt gaaattttgg attatctttg ctgttcagtc ctttttacca
    35221 attctgactt tgctcggtct ttctctatat ctaggcttgt ccatcctttc gatgatttac
    35281 cttattgtgg catggatcct tgccagtgtc atcctttctt gccaccatta ctttaaggac
    35341 gttaaaaatc tgtcaaccaa ttggagtagc attacggacc tggtgaatcg ttcaaatggc
    35401 atagtcgcca tagttttatt gtttatttat agtgcaatcc tgatggccct tgtaattggg
    35461 agcatattct tggttcagtc tctctcccct atccttgcca tcagcttggg agtaggagct
    35521 cttatcctcc tgcttgctct tgctattttt ggctatcatt attacctgtc acgcatattg
    35581 gcagaaatag aaaaaagatg aactggttgg tcgcttgctt gataaattca attcgacaaa
    35641 cgcttttata gtttgttaga atgcagttgt aaatgattaa gattggtaat caaaaatata
    35701 gggaggaatg ttttgaataa gaaatataac atagttctat ttttgttgtt tatagtttat
    35761 ttattgggat atttttcaat ttcaaaaacg ttaatcccta taatgtgtgt tccaagtatt
    35821 tttgatagaa catatattta gagttcgaaa tagaatgatg cagataggtg aaattataat
    35881 tattgttgct tctataatat tatttattga tagtatattg agtttgtaac aaaaggcttc
    35941 tctataaaat caaattttcc agatacctac tgaagctgat ttatttcttt atctttcttc
    36001 tactaccttt gtcactaaat ttgacagaca catattgctg atacaacctc tctacctatt
    36061 aatctcacag ttaacctgag gctagtttca tagtttgcta tttgattttc gttgataaaa
    36121 aaaatgctcc tccaagacgg aagagcattt tttattgtga ttcttacaca tttacttcct
    36181 agttttgcag gaagtctagt ataccatttt ttaaaagaag agtaaaaaag caagggtagt
    36241 ttcttttttg agaattgtct aaaagtttga tataatggta gttatgaata gaataagagt
    36301 cagcaaaagg gttgaaaaga agcttgctaa ggggctagtt ttactagaag ccagtgatct
    36361 tgagaatgtc aatcttaagg atcaggaagt agaggtgcag ggtcaggaag gaaactttct
    36421 tgggactgcc tacctttctc agcaaaacaa gggcttgggc tggtttgtca gcaaagacaa
    36481 ggtggccttc aatcaaactt tctttgaaac gttgtttaga aaagccaaag aaaagagaaa
    36541 cgcctactat caagatgatt tgacaactgc ctttcgtctc tttaatcaag agggagatgg
    36601 ctttgggggt ctgacagtgg acctttatgg cgactacgcc gtcttttctt ggtataactc
    36661 ttatgtttat cagattcgtc agactatatc agaagccttt agacaggttt tccctgaggt
    36721 tttaggggct tatgagaaaa tccgctttaa gggttttgac tatgaatctg cccatgttta
    36781 tggtcaagaa gcacctgact ttttcactgt tttagaaaat ggtgtcctgt atcaagtctt
    36841 tatgaatgat ggcttgatga caggaatttt cctagaccag catgaggttc gcggtagttt
    36901 agttgacggc ttggctatgg gtaaatcctt gctcaatatg ttttcctaca cagcggcttt
    36961 ttcagtagct gcggccatgg gaggagctag ccagacaact tctgttgatc tagccaaacg
    37021 ttcacgagaa ttgtctcaag cgcattttca ggcaaatggg ctcagcacag acgagcatcg
    37081 ttttatagtc atggatgtct ttgagtattt caaatatgcc aaacgcaaag acttgaccta
    37141 cgatgtgatt gtcctagatc cgcctagctt tgctcggaat aaaaaacaaa ctttctctgt
    37201 ggccaaggat tatcacaagt tgatttccca gagtcttgag attttaaatc cgggagggat
    37261 tatcattgcc agtaccaatg ctgccaatgt ttcccgtcag aaatttacag aacaaattga
    37321 taaaggcttt gcaggaagaa gttaccagat tttaaacaaa tatggtcttc cagcagattt
    37381 tgcctataat aaaaaagatg aaagtagtaa ttacctcaag gtgattagta tgaaggttag
    37441 taaatgaaat taatcgtttc agtaatgcca agaagtttag aggaggctca ggctctggat
    37501 gccacgaggt acctggatgc cgacatcatt gaatggcgtg ccgactatct gcctaaagaa
    37561 gcgattttgc aggtagctcc agccattttt gaaaaattcg caggccgtga gttggttttc
    37621 acgctacgaa ctcgctccga aggtggagaa atcgaccttt ctccagaaga gtatatccat
    37681 ctaatcaagg aagttgcgca attctatcaa ccagactata ttgattttga gtactatagc
    37741 tacaaggatg tttttgagga aatgctggac ttcccaaatc ttgttttgag ttaccataat
    37801 ttccaagaaa cacctgagaa catgatggaa atcttgtcag agttgacgat cctaaatcca
    37861 aaacttgtta aggttgcggt gatggctcac acggagcagg atgtcttaga cttgatgaac
    37921 tatacacgag gctttaaaac cctcaatcct gaacaggaat atgtgaccat ttctatgggt
    37981 aaggtgggca aggtctctcg tatcactgcg gatgtgactg gttcgagttg gtcttttgcc
    38041 agtctggatg aggttagtgc ccctgggcag atttctctag ctagcatgaa aaaaatcagg
    38101 gagattttgg atgaagcttg atggctatac acgtttagct gcagttgttg ccaatcctat
    38161 taagcattct atttctccct tcatccacaa tagagccttt gaggcgacag ctaccaacgg
    38221 tgcttatgtg gcttgggaga ttgaagcgag tgacttggta gaaacagtgg ccaatattcg
    38281 tcgctaccag atgtttggca tcaatctgtc catgccctat aaggagcagg tgattcctta
    38341 tttggataag ctgagcgatg aagcgcgctt gattggtgcg gttaatacgg ttgtcaatga
    38401 gaatggcaat ttaattggat ataatacaga tggcaaggga ttttttaagt gcttgccttc
    38461 ttttacaatt tcaggtaaaa agatgaccct gctgggtgca ggtggtgcgg ctaaatcaat
    38521 cttggcacag gctattttgg atggcgtcag tcagatttcg gtctttgttc gttccgtttc
    38581 tatggaaaaa acaagatctt acctagacaa gttacaggag cagacaggct ttaaagtgga
    38641 tttgtgtgct ttagaatatg tttctgaact gcaagcaagg attgccgagt cggatttgct
    38701 agttaatgcc accagtgtgg gcatggatgg ccaattctct cctgttcctg aaaacatagt
    38761 cttaccagaa actctcttag ttgcagatat catataccaa ccctttgaaa caccgttttt
    38821 gaaatgggct agaagtcagg gcaatccagc agtcaatggt ctgggaatgt tgctctatca
    38881 agctgcagaa gcttttcaac tgtggacagg caaggaaatg ccgacagaag agatttggca
    38941 gtctttaaca gaaaaatacc aataaagaaa gggagactct cctatgaaaa tcagaatcga
    39001 tattcctcat catccttatg atattcagat tgaaaaaggt tgtatggccc aggctggtca
    39061 gtggttgcga gaactctggc aaccgcaaaa ggtagtcatt gtgacagata accatgtagc
    39121 ctctctctat gcagagaagg tcaagctcag cctagaagat gctggttttc aggtagctgt
    39181 ttttgatttc ttagaaggtg aagaaagaaa gaatttaact actgttcaga aagtctatga
    39241 atttctagtc aagcaaggtc tgactcgtag cgatggaatc gttgctcttg gtggtggcgt
    39301 tgttggggac ctggctggat ttgtagcctc tacctatatg cggggtattc actttgttca
    39361 gattccgact agtttgacag ctcaggttga ttcttctatc ggtggaaaga caggcgttaa
    39421 cacaccattt gctaaaaata tggtggggac ctttgcccaa ccagatgggg ttctgattga
    39481 tccacttgtt cttgaaaccc tcggaaaaag agagttgatt gaagggatgg gtgaagttat
    39541 caagtatggc ttgattgagg atccagaact gtgggctctc ttgacggggc tgaatggttc
    39601 tgttgagagc attttggaac atgcagagac cttgattgaa cattcttgtc aggtgaagcg
    39661 caagatggtg gttgaagatg agttggacaa tggtattcgt ctttacctca attttggcca
    39721 cactattggc catgccatcg aagcgactgc cggttatggc aaggtcatgc atggagaggc
    39781 tgttgccatg ggaatggtac agatttccaa gattgctgag gaaaaaggcc tcatgccagc
    39841 tggcattacc caatctatca cagagatgtg tcagaaattc ggcttgcctg ttgactatga
    39901 aaattgggaa gttgacaagc tttatcaggc tcttactcat gacaagaaag cgcgtggtaa
    39961 caccttgaaa ttggtcttgg tgccagagct tggttcagcg accattcatc cagtttctct
    40021 ggaagagatg aaagactact tggtaaaata aggagagtgt atgagatatt taactgcagg
    40081 agaatcacac ggccctcgtc taacagctat tattgaggga attccagctg gacttccatt
    40141 gacagctgag gatatcaatg aggaccttag acgccgtcag ggtggctacg gtcgtggtgg
    40201 tcgtatgaag attgagagtg accaggttgt ctttacttcg ggcgttcgcc acgggaagac
    40261 gacaggggcg cctattacta tggatgtcat caataaggac caccagaaat ggctggacat
    40321 catgtctgcg gaggacattg aagaccgcct taaaagcaag cggaaaatta ctcatcctcg
    40381 cccaggtcat gccgatttgg ttggggggat taagtaccgt tttgatgatt tgcgaaattc
    40441 tttggagcgt tcatcagctc gtgaaaccac catgcgggtg gcagttggtg cagtagccaa
    40501 acgtctcttg gctgagctgg atatggagat tgccaaccat gtcgtggtct ttggtggcaa
    40561 ggaaatcgat gttcctgaaa atctgacagt cgctgaaatt aagcaaagag ctgcccggtc
    40621 tgaagtttct attgtcaacc aagaacgaga acaggaaatc aaggactata ttgaccaaat
    40681 caaacgtgat ggtgatacca tcggtggggt tgtggagaca gtcgtcggag gcgttccagt
    40741 tggtcttggt tcctatgtcc aatgggatag aaaattggat gcaagattgg ctcaagctgt
    40801 tgtctctatc aatgccttta aaggggtgga atttggtctt ggctttgagg ctggttatcg
    40861 taaaggcagc caagttatgg atgaaattct ctggtctaaa gaagacggtt atactcgccg
    40921 taccaataat ctaggtggtt ttgaaggtgg tatgactaat gggcaaccca tcgttgttcg
    40981 tggggttatg aaacccattc ctactcttta taaacctctt atgagtgtgg atatcgaaac
    41041 tcacgaacct tacaaggcaa ccgtggagag aagtgatccg actgctcttc cagctgcagg
    41101 aatggtcatg gaagcagttg tagcaacggt tctggcgcaa gaaatcctcg aaaaattctc
    41161 atcagataat cttgaggaac taaaagaagc ggtagccaaa caccgagact atacaaagaa
    41221 ctattaagga gttcctatgg caaaaacaat ctatatcgca ggtcttgggt tgattggagc
    41281 ctctatggca cttggtatca aacgcgatca tccagattat gaaattttag gttataatcg
    41341 tagtcaagct tcgagagaca tcgccttgaa agaaggcatg attgaccgtg caacggatga
    41401 ttttgctagt tttgctcctt tggcagatgt cattatcctc agcttgccaa tcaaacaaac
    41461 tattgctttc attaaggagt tggccaattt ggatttgcga gaaggcgtta ttatttcaga
    41521 tgctggttcg accaagtcaa ccattgtgga tgcggcggag cagtatttgg ctggcaagtc
    41581 tgttcgcttt gtcggggccc atcccatggc tggtagtcac aagacaggag ctgcttcggc
    41641 agatgtcaat ctttttgaaa atgcctatta tatctttaca ccttcaagcc tgacaagtca
    41701 ggacacgctt aaggaaatga aggatctgct ttcaggtctt catgctcgtt ttatcgagat
    41761 tgatgccaag gagcatgatc gtgtcacttc tcagattagc cattttcctc atattttggc
    41821 ttctagtctc atggagcaga ctgcggtcta tgctcaagag catgagatgg caaggcgctt
    41881 tgcggcaggt ggttttcgag atatgacccg aattgcggaa agcgagccag gaatgtggac
    41941 ctccattctc ttgtccaata gcgagatcat tctggataga attgaggatt tcaaggaacg
    42001 tttggaagcg attggtcagg ccattagtaa gggagatgaa gagcaaattt ggaacttttt
    42061 taaccaagcg cgtgaacaac gccagaccat ggaaatccat aagcgtggtg gtgtggatag
    42121 ctcttacgac ctctatgttg acgttcctga tgaagaagat gtcatcctgc ggattttgga
    42181 actgctacgt ggaacttcct tggttaatat ccacatcaac gaggaaaatc gtgaggatat
    42241 tcacggaatc ctacaaattt catttaaaaa tgctcaagac ttggaaagag ctgagtatct
    42301 cataacagaa aataccgact atacagtcgt catcaaataa ggagaagaac atgtcaaata
    42361 tttacgatag tgcaaacgaa ctcagtcgcg gtctacgtgg attaccagaa tacaaggctg
    42421 ttaaagcagc taaagatgcg attgcagcag atgctgaggc aagcaaaatc tttacagaat
    42481 atcttgcctt tcaagaggaa attcaaaaac tagcacatac aggtcaaatg ccagatgcgt
    42541 ccttccaagc taagatggaa ggctttggta aacagattca aggaaatagc ctcttgtcag
    42601 aattctttac caagcaacaa caattggcca tttacctttc tgacattgaa aaaattgttt
    42661 tcgaaccagt ttcagaattg ctgaaataag aaaagaacga tcctaaagtc aggaattttt
    42721 aaggaatttc tggcttttta tgataaaata ggtaaaagta ttgcaagtat gaggtccagt
    42781 atgaaactaa aaacaaacat tcgccattta catggtagta tccgcgtccc aggtgacaag
    42841 tctatcagcc accgttccat tatctttgga agtttggctg agggtgagac caaggtttat
    42901 gatattctgc gaggtgaaga cgttctttcg accatgcagg tttttcgtga ccttggtgtt
    42961 gaaattgagg ataaagatgg ggttattacc attcaaggtg taggcatggc tggcttaaaa
    43021 gcgccgcaaa atgcccttaa tatgggaaat tctggcacct cgattcgcct gatttcaggt
    43081 gtccttgctg gtgcagattt cgaagtagag atgtttggag atgatagtct ttccaaacgt
    43141 cctatggacc gtgtgaccct tccactgaaa aaaatgggcg tcagcatttc agggcaaact
    43201 gaacgagact tgcctcccct tcgcttaaaa gggacgaaaa acctaagacc tattcattat
    43261 gagttgccaa ttgcctctgc ccaagtcaag tcagccttga tgtttgcagc cttacaagct
    43321 aagggggagt cagttattat cgaaaaagag tgcacccgta atcatactga agatatgttg
    43381 aaacaatttg gtggtcattt aagtgtggac ggtaagaaaa tcacagtcca agggccacaa
    43441 aaattgacag gacagaaggt ggtcgtacca ggagatattt ccagtgcagc cttttggtta
    43501 gtcgcaggtt tgattgttcc aaattctcgt ctagtgctgc agaatgtggg gatcaacgaa
    43561 actcgcaccg gtattattga tgtcattcgt gccatgggtg gaaaattgga aataactgaa
    43621 atcgatccag tcgctaaatc ttctaccttg actgttgagt cttctgactt gaaaggaaca
    43681 gagattggtg gcgctttgat tccacgattg attgatgaat tgcccattat tgccctgctt
    43741 gcgactcaag cccaaggtgt aacagttatc aaggatgctg aggagctcaa ggtcaaggaa
    43801 acagaccgta ttcaggttgt ggcagacgcc ttaaatagta tgggagcaga tattactcct
    43861 acggcagatg ggatgattat caaagggaaa tcagctcttc acggtgctag agtcaatacg
    43921 tttggtgacc accgtatcgg catgatgaca gctatcgcag ccctcttggt tgcagatgga
    43981 gaggtggagc ttgaccgtgc agaagccatc aataccagct atcctagttt ctttgatgat
    44041 ttggagagct tgattcatgg ctaaggtgtt attagggttt atgggggctg gaaaatcgac
    44101 tattgcaaga ggcttggacc ctaattacct tgatatggat gctctgattg agaagcgcct
    44161 aggtatgtcc attgcgaatt ttttcgctga aaagggagaa gcagcctttc gtcaggtaga
    44221 atcagaagtc ctagctgatt tactacaaac agaccaagtc gtgtcaactg gaggaggagt
    44281 ggttatttct cagagaaatc gtgacttact caagactaat acagataaca tctacctgaa
    44341 agcagatttt gaaaccctct accaacgtat cgcagctgat aaggacaatc agcgaccgct
    44401 ttttctaaat aatagcaagg aagaactagt agctattttt caagaaagac aggcttggta
    44461 tgaggaagtg gctagtcggg ttttggatgt gaccaagcta agcccagagg aaattataga
    44521 ggaactaaga tgaaaattgc ttatctaggt cccaagggat cattttcaca ccacgttgtg
    44581 cagatagctt ttcctcatga ggaattgcag gcttttgcca acattacaga tgtcatcaag
    44641 gcttatgagc aaggattagt ggactattct gtggtgccag ttgaaaattc tattgagggt
    44701 agtgttcatg aaaccttgga ctatcttttt catcaggctc acatccaagc agtagcagaa
    44761 atcgttcagc ctattcatca gcagttgatg gtggttccag gccatactaa gattgaaaag
    44821 attttttcac atccacaggc cttggctcaa ggaaagaaat tcatcgatga acaatatcca
    44881 gaggctcaaa tcgaggtaac agctagtaca gcttatgcgg cccgttttat ttccgaacat
    44941 ccagatcagc cctttgcagc aattgcacct agaagttctg ttgaagaata tggattggaa
    45001 ctgattgccg aggatattca ggaaatggaa gccaatttca cacgtttctg ggttctagga
    45061 gctgaaaagc ctagtattcc cttgcaagca caaactgaaa agatgagttt ggccttgaca
    45121 ttacctgaca accttccagg tgcactttat aaggccctgt cgacctttgc ttggcgagga
    45181 attgacttga caaaaattga aagtcgtcca ctcaagacag cactgggtga atactttttc
    45241 attatcgatg tggattatac cgataaggac ttggtccact ttgcccaaaa agaattagaa
    45301 gcgattggaa tccagtataa aattctgggt acctatccta tttatccaat atcagaccat
    45361 ggaaaggaga gaagatgagt aaagaaaatc ccttaagtca tcatgagcag ttgcgttatg
    45421 attatttgct aaaaaatatt cactatctca atgagagaga aaaaaatgag tttgcctatt
    45481 tgcaagaaaa gctaactctt gctaggggaa atagtagctc tagcttggaa caagaaagag
    45541 aagagcaggt tgacttacca agctatgcga accggagtcg ctcacaatcc aaatcacaag
    45601 cactctcttt ccctccaaaa aagaaaagac ggaagctccg tcttaaacga atttttatgg
    45661 tgattttttc acttttggtc tgtgtggctt tggccatggt attcatgttt ttgcgtggtt
    45721 accaagatgc tagcgcaaag aaaactgctg atgcccgggc agctcaagta gaagtcttta
    45781 atggtcagga cactagagat ggagttaata ttttaatcat gggtactgat ggtcgaatcg
    45841 gccagaacag tgttgagaca cgaactgact ctattatggt attaaatgtc gggggctcag
    45901 ataagaaaat gaagctagtc agtttcatgc gtgataattt ggtctatata gatggttata
    45961 gtcaagtgat taacggtaga aaacagacag ataacaagtt aaacgtagcc tacgagttag
    46021 gagaacaaga ggggcaaaaa ggggcagaaa tggttcgcca agtcttgaaa gataattttg
    46081 acttggacat taagtactac gccttggttg atttccaggc ctttgccaca gcgattgaca
    46141 cgcttttccc tgatggggtg acgattgatg ctcaattttc aacattgaat gggcgtcccc
    46201 taacagaagc tacagtcgga gatgatttat acgctaccga gactgagtct ccaacccaaa
    46261 ccattaaagt cggaaaacag cagatgaatg gctcgaccct gctcaattat gctcgtttcc
    46321 gtgatgatga tgaggcggat tacggccgta caaaaagaca gcaacaagtt ttaacagcaa
    46381 ttttagagca aattaaagat cccactaaac ttttcactgg ttctgaagct cttggaaagg
    46441 tttttgctat gacctcaacc aacgtaccct atactttcct cctaacaaac ggtttatctg
    46501 ttttggatgg agcaaaaaat ggtattgaaa aattgacgat tccagaactg ggtgactggg
    46561 tagatgccta tgatgtttat ggaggcttgg gcttgctggt tgatcaaaac aaatatcaaa
    46621 ccaagctcgc tcaaatgggc ttgagataag ataatactca atgaaaatca aagtgcaaac
    46681 taggaagcta gccgcaggct gctaaaaaca cagttttgag gttgtagata agactgacga
    46741 agtcagttac ctatacctac ggcaaggtga agctgacgtg gtttgaagag attttcgaag
    46801 agtataagac agaaaaatca aagtttgata gctatttgga tagcagtcag gctttgattt
    46861 tttacagtct gcaatagatt ttcacccctt gtttgtggta taatgaaacg atacgataga
    46921 aagaataatg aacaatatga ctgatttaaa agcaattcag gctcgtagtc tggagatggc
    46981 tgaatatttt gtggcctttt gtaaagaaca tgatttgctc tgttatctct gtggcggagg
    47041 cgctattggt gcccttcgaa acaagggatt tattccttgg gacgacgacc tagacttttt
    47101 tatgcctcgt aaagattatg agaaattagc agaattatgg cctcgttatg cagatgaacg
    47161 ttatttcttg tcaaagagtc acaaggattt tgttgatcgt aatcttttta ttaccattcg
    47221 tgacaaggaa accacctgta tcaagcctta tcagcaggat ttggatttgc cacatggtct
    47281 ggccttggat gttttgcctt tggattatta tccgaaaaat ccagctgagc ggaaaaaaca
    47341 ggttcgttgg gccttgattt attcactctt ttgtgcgcaa actattccag aaaagcatgg
    47401 tgctctcatg aaatggggaa gtcgcatttt actgggtttg actccaaaat ctctctgtta
    47461 tcgcatctgg aaaaaagctg agaaagaaat gactaagtat gatttggctg attgtgatgg
    47521 cattacagaa ttatgctcag gtcctggcta catgagaaac aagtacccaa tcacatcttt
    47581 tgaagacaat cttttcttgc catttgaagg aacagagatg cctattccaa tcggctatga
    47641 tgtctatctc agaactgctt ttggggatta tatgacgcct ccaccagcag acaagcaggt
    47701 accgcatcat gatgctgtca tcgctgatat ggataagtct tatacagaat acaagggaga
    47761 atatggtggc taagaaaaaa atcttatttt ttatgtggtc tttttctctt ggaggtggtg
    47821 cagagaagat tctatcaacc attgtttcaa atctggatcc agaaaagtat gatattgata
    47881 ttcttgaaat ggagcacttt gacaagggat atgaatctgt tccaaagcat gtacgcattt
    47941 taaaatccct tcaagattat cgccaaacca gatggttacg agcttttttg tggagaatga
    48001 gaatttattt tccaagactg gctcgtcgtt tgcttgtaaa agatgattat gatgttgaag
    48061 tttcttttac cattatgaat ccaccactgt tgttctctaa aagaagagaa gtcaagaaga
    48121 tatcttggat tcatggaagt attgaagaac ttcttaagga tagctctaaa agagaatcac
    48181 atagaagcca gttggatgct gcgaatacaa ttgtagggat ttcaaaaaag accagcaatt
    48241 ctatcaagga agtttatcca gattatactt ctaaattaca gacaatctac aatggatatg
    48301 attttcagac tattctagaa aaatctcaag agaagatcga tatcgagatt gctcctcaaa
    48361 gtatctgtac tatcggacgg attgaggaaa ataagggttc tgaccgtgta gtggaagtga
    48421 tacgattatt acaccaagag ggaaaaaact atcatctcta ttttatcggg gctggtgata
    48481 tggaagagga actgaaaaaa cgagtcaaag agtatgggat tgaggactat gtacatttcc
    48541 ttggttatca aaaaaatcct tatcagtatc tatctcagac gaaagttctc ttgtctatgt
    48601 ctaaacaaga aggttttcct ggagtgtatg tggaggcctt gagtctggga ctccctttta
    48661 tctctacgaa cgttggaggg gctgaggaat tatcccaaga aggacgattt ggacaaatca
    48721 ttgagagcaa tcaagaggca gctcaggcga ttactaatta catgacttct gcctcaaact
    48781 ttgatgtcga tgaggctagc caattcattc aacaatttac aattacaaaa caaatcgaac
    48841 aagtagaaaa actattagag gagtagcatg gaaactgcat taattagtgt gattgtgcca
    48901 gtctataatg tggcgcagta cctagaaaaa tcgatagctt ccattcagaa gcagacctat
    48961 caaaatctgg aaattattct tgttgatgat ggtgcaacag atgaaagtgg tcgcttgtgt
    49021 gattcaatcg ctgaacaaga tgacagggtg tcagtgcttc ataaaaagaa cgaaggattg
    49081 tcgcaagcac gaaatgatgg gatgaagcag gctcacgggg attatctgat ttttattgac
    49141 tcagatgatt atatccatcc agaaatgatt cagagcttat atgagcaatt agttcaagaa
    49201 gatgcggatg tttcgagctg tggtgtcatg aatgtctatg ctaatgatga aagcccacag
    49261 tcagccaatc aggatgacta ttttgtctgt gattctcaaa catttctaaa ggaatacctc
    49321 ataggtgaaa aaatacctgg gacgatttgc aataagctaa tcaagagaca gattgcaact
    49381 gccctatcct ttcctaaggg gttgatttac gaagatgcct attaccattt tgatttaatc
    49441 aagttggcca agaagtatgt ggttaatact aaaccctatt attactattt ccatagaggg
    49501 gatagtatta cgaccaaacc ctatgcagag aaggatttag cctatattga tatctaccaa
    49561 aagttttata atgaagttgt gaaaaactat cctgacttga aagaggtcgc ttttttcaga
    49621 ttggcctatg cccacttctt tattctggat aagatgttgc tagatgatca gtataaacag
    49681 tttgaagcct attctcagat tcatcgtttt ttaaaaggcc atgcctttgc tatttctagg
    49741 aatccaattt tccgtaaggg gagaagaatt agtgctttgg ccctattcat aaatatttcc
    49801 ttatatcgat tcttattact gaaaaatatt gaaaaatcta aaaaattaca ttagaacggg
    49861 ggtgaccaag tgattgatgg gaaacgatta ttatttagtt tgaccatagt cagttatgcc
    49921 ttgacgctag taagtggagt tgtgtatctg tttaataata ataatgttag cttactttct
    49981 actttattgt tcttgttggt tagtagctta attgcttgtt ggaatgatat caagtattac
    50041 ttaatccatt ttattttcta tttaaccatt tttgtatttc tggtatcaag accgaccatt
    50101 gattatttta gggatggtgc tttggatacc tatcatccaa tagcctatcg ttttgccttt
    50161 atagttgtca tggtttcgat tctgggcttg accacaggag gcattctggc tcgttacttc
    50221 atagctagga agaaaataaa agtagcaaat ataggaaatt ctctaaaaga ggtttatatc
    50281 aagcggttac gctttgtatc actaggagtt tttcttctaa cctatccttt ctatttcatt
    50341 cggttatttg aacggctctt gtatcgtttg cagacttcct actatgccta ctatgcaaat
    50401 tttgaaagta aactgcctta ttttacctac attttgtcta cctttacggt ctatgcaatg
    50461 tgtatgtatc tggcaaccaa gccaaagaaa ttgcaggcca cagcagtgct tgtctccttt
    50521 attgcagcta atactattca tttggcaatt gggacacgaa atccctttat tttaagtatt
    50581 ttatttgctt ttgtttatta ctttatgcgg gagcaaactg aaaaaggaaa atggattggg
    50641 tttaaagaaa agttagcgat ttttgtaggt tctcctattc tcatgttagc gatgggagta
    50701 ctcaattatg tacgggataa tgtccaagtt tcccatacag gtttctggga tatcttactt
    50761 gactttatct ataaacaagg gactagtttt ggtgttctgg ctcgaggttt tctatttaac
    50821 agtagcctcc cttaccgaga tttccgtaat tttacttttg gtcctgttct tgattatttt
    50881 gcaaggggga gtttgggagc cattttcgga ggaaaagcct ttgaacatac aaccaatagt
    50941 gtggaactag ctattgatag taatagttat gcccacaatc tatcctatct tgtcttgaac
    51001 aaggaatact tgaaagggca tggtatcgga agtagttata tcatggagtt gtataccgac
    51061 tatggtatga ttggagtctt tctgcttagt ttcttactcg gcgtattatt tatagccatg
    51121 ctgcaagtag cctatcgctc aaggacaatc ctatttgctt tatccctact catcttgaat
    51181 aatctattct ttatgccaag aagcagcttt tcagaaagtt tcttcaattt atttacaatg
    51241 caattctggg gaattgttct tgtgattata tttgtagcaa aaatgcttac aaaggaaaac
    51301 tagtatctac taaacaaagg agaaaaaaat catgtttgaa cattattcag tagctgattt
    51361 gtttgcaaat ctttataaaa agcgaaaagc aaatatttta gctctcatcg ctttatttgc
    51421 tctcattgct gtaccattta caattaaagc agttaggaat aaaaacactg tcaaagatac
    51481 aacaagttat tcaacttatc ttatctataa aatcactcct ccaaaagagt cggccaaaac
    51541 gattttgaat catcaaattg gtggttatag tgatttttat gggaaattga ttgatggtaa
    51601 tttgaatgga gcttatcttt tcaatgatgt agaacccagt gagttgaaaa aaattgccag
    51661 tgaattagac acgacagaaa caaccttgaa aaattcaacg aatgactatt ggtggaaaaa
    51721 attgaccgtc tactatatga ttgacgatgc aggggttggt gtgaaaattt tgacatcaag
    51781 taaagatgcc aatgacttgt tagagaaaaa aattgatggg ttgattgaga aatttaaaca
    51841 tgcctatgca aatgtgaaaa ttgaaaaact ggaaaccatc aactctaaag aattgaacgc
    51901 aaatggtgaa acagcgcttg gcttaaatgt gaaaaatctg attcttcgtt tagttgttat
    51961 tggagtggtt tgtgtgattt tggttgtgat gggaaatgtg ttagtttatc tctttaatcc
    52021 aacaatcaat agagtaggtg atttttctca gtatcaaatt gattttgtaa cagagattac
    52081 aacaattgct aacctagcag atgttttgtc atacaaaaat actggacagg aattgaccat
    52141 cgttagctca aataaagcta tcctagataa attgaaacag agtcaagaag ctttaaaagg
    52201 aatgcatttt gtagatttac aggatgtatc atctcttttg gaaagagata cagtccttct
    52261 tgttgaagag tacggagtga ctcgttataa gaaatttgag caaagtcttc aaattctcag
    52321 aaacttaaat cgttctatcc ttggtgtagc aacctttaaa ttataagtat tctgatgtat
    52381 aaggtcttca aaatcggcta agagctgatt ttgaaggctt tttctatctg tctgcagaaa
    52441 ttttcttttg gagtctccta tagaaagacc ctagggaaaa tctatccgta tagaggagcc
    52501 ttcttttgat aaaatcgtaa aaatctagta taatagatag aactgaaagt atgaggttac
    52561 tagcaatgaa aatgaaacaa attagtgata caactttaaa aatcacgatg tctttagagg
    52621 atttgatgga tcgtggaatg gagattgctg actttctcgt tcctcaagaa aaaacagaag
    52681 agttctttta tgctatcttg gatgaactag agatgcctga tagctttctg gatacaggca
    52741 tgttgagctt ccgtgtgact ccaaaacctg ataaggtaga tgtctttgtg accaagtcaa
    52801 agattgacca aaatctagat tttgaagact tatcggattt gccagatatg gaagaattgg
    52861 ctcaaatgtc tccagatgaa tttatcaaaa ccctggaaaa aagcatcgca gacaaaacca
    52921 aggatgatat cgaagccatt caatctcttg agcaagttga agccaaggaa gaagagcaag
    52981 agcaggctga acaagaagct gagagtaaga aagaacctta catctactac atcctttctt
    53041 ttgctaagtt ggctgacttg gtagtttttg ccaagacagt gacttttgaa atggaaactt
    53101 ctgaactcta caaaatgaac gagcgctatt atttgaccat tttagtggat attgaaaatc
    53161 atccaagccc atatccagct tggctgttgg cccgtatgcg cgagtttgca gacgatagtg
    53221 atatcagtcg ctcagtctta caagagtatg gtcaagtctt gatgagtcac gatgcagtgc
    53281 tcaatctgca aaaaatcggc taatcctttc tggaaagcta ttttcagatt ttaagaagag
    53341 cgaatcgtct gattcgtttt ttctttttat actgaaatag tgatttacta taataggaat
    53401 tttcacaaaa ttctgttata atggctatat tagaaaattt cgaggagata aagatgacag
    53461 ttaaaattgc tttactagga tttggtaccg ttgcaagtgg tgtgcctttc ctcctaaagg
    53521 aaaatggagg aaaaatcaat caatcagcac attcagatat caaagttgct aaggtattgg
    53581 tcaaggatga agatgaaaaa aatcgcttgc ttgcagcagg gaatgacttt aactttgtaa
    53641 ccaatgtgga tgatatttta tcagaccagg atattactat cgtagtggaa ttgatggggc
    53701 gtattgagcc tgctaaaacc tttatcactc gtgccttgga agctggaaaa cacgttgtta
    53761 ctgctaacaa ggacctttta gctgtccatg gagcagaatt gctagaaatc gctcaagcta
    53821 acaaggtagc actttactac gaagcagcag ttgctggtgg gattccaatt cttcgtactt
    53881 tagcaaattc cttggcttct gataaaatta cgcgcgtgct tggagtagtc aacggaactt
    53941 ccaacttcat ggtgaccaag atggtggaag aaggctggtc ttacgatgat gctcttgcgg
    54001 aagcacaacg tctaggattt gcagaaagcg atccgacgaa tgacgtagat gggattgatg
    54061 cagcctacaa gatggttatt ttgagtcaat ttgcctttgg catgaagatt gcctttgatg
    54121 atgtagccca caagggaatc cgcaatatca caccagaaga cgtagctgta gctcaagagc
    54181 ttggttacgt agtgaaattg gttggttcta ttgaggaaac ttcttcaggt attgctgcag
    54241 aagtgactcc aaccttccta cctaaagcgc acccacttgc tagtgtgaat ggcgtaatga
    54301 acgctgtctt tgtagaatct atcggtattg gtgagtctat gtactacgga ccaggtgcgg
    54361 gtcaaaaacc aactgcaaca agtgttgtag ctgatattgt ccgtatcgtt cgtcgtttga
    54421 atgatggtac tattggcaaa gacttcaacg aatatagccg tgacttggtc ttggcaaatc
    54481 ctgaagatgt caaagcaaac tactatttct caatcttggc tctagactca aaaggtcagg
    54541 tcttgaagtt ggctgaaatc ttcaatgctc aagatatttc ctttaagcaa atccttcaag
    54601 atggcaaaga gggtgacaag gcgcgtgtcg ttatcatcac acacaagatt aataaagccc
    54661 agcttgaaaa tgtctcagct gaattgaaga aggtttcaga attcgacctc ttgaatacct
    54721 tcaaggtgct aggagaataa gatgaagatt attgtacctg caaccagtgc caatatcggg
    54781 ccaggttttg actcggtcgg tgtagctgta accaagtatc ttcaaattga ggtctgcgaa
    54841 gaacgagatg agtggctgat tgaacaccag attggcaaat ggattccaca tgatgagcgt
    54901 aatctcttgc tcaaaatcgc tttgcacatt gtaccagact tgcaaccaag acgcttgaaa
    54961 atgaccagtg atgtcccttt ggcgcgcggt ttgggttctt ccagctcggt tatcgttgct
    55021 gggattgaac tagccaacca actgggtcaa ctcaacttat cagaccatga aaaattgcag
    55081 ttagcgacca agattgaagg gcatcctgac aatgtggctc cagccattta tggtaatctc
    55141 gttattgcaa gttctgttga agggcaagtc tctgctatcg tagcagactt tccagagtgt
    55201 gattttctag cttacattcc aaactatgaa ttacgtactc gcgacagccg tagtgtcttg
    55261 cctaaaaaat tgtcttataa ggaagctgtt gctgcaagtt ctatcgccaa tgtagcggtt
    55321 gctgccttgt tggcaggaga catggtgacc gctgggcaag caatcgaggg agacctcttc
    55381 catgagcgct atcgtcagga cttggtaaga gaatttgcga tgattaagca agtgaccaaa
    55441 gaaaatgggg cctatgcaac ctacctttct ggtgctgggc cgacagttat ggttctggct
    55501 tctcatgaca agatgccaac aattaaggca gaattggaaa agcaaccttt caaaggaaaa
    55561 ctgcatgact tgagagttga tacccaaggt gtccgtgtag aagcaaaata aagaatagaa
    55621 gataggatgg ggaaactctt gaccagaggg tttcatatcc tttttgtgaa aagaagttta
    55681 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgctc aaaacagtgt
    55741 tttgaggttg tggatagaac tgacgaagtc agctcaagac actgttttga ggttgcagat
    55801 agaactgacg aagtcagtaa catatatacg gtaaggtgac gctgacgtgg tttgaagaga
    55861 ttttcgaaga gtattagtta aaaacgtgat aaaggagaaa taaagatggc agaaatttat
    55921 ctagcaggtg gttgtttttg gggcctagag gaatattttt cacgcatttc tggagtgcta
    55981 gaaaccagtg ttggctacgc taatggtcaa gtcgaaacga ccaattacca gttgatcaag
    56041 gaaacagacc atgcagaaac ggtccaagtg atttacgatg agaaggaagt gtcactcaga
    56101 gagattttac tttattattt ccgagttatc gatcctctat ctatcaatca acaagggaat
    56161 gaccgtggtc gccaatatcg aactgggatt tattatcagg atgaagcaga tttgccagct
    56221 atctacacag tggtacagga gcaggaacgc atgctgggtc gaaagattgc agtagaagtg
    56281 gagcaattac gccactacat tctggctgaa gactaccacc aagactatct caggaagaat
    56341 ccttcaggtt actgtcatat cgatgtgacc gatgctgata agccattgat tgatgcagca
    56401 aactatgaaa agcctagtca agaggtgttg aaggccagtc tatctgaaga gtcttatcgt
    56461 gtcacacaag aagctgctac agaggctcca tttaccaatg cctatgacca aacctttgaa
    56521 gaggggattt atgtagatat tacgacaggt gagccactct tttttgccaa ggataagttt
    56581 gcttcaggtt gtggttggcc aagttttagc cgtccgattt ccaaagagtt gattcattat
    56641 tacaaggatc tgagccatgg aatggagcga attgaagttc gttctcgttc aggcagtgct
    56701 cacttgggtc atgttttcac agatggaccg cgggagttag gcggcctccg ttactgtatc
    56761 aattctgctt ctttacgctt tgtggccaag gatgagatgg aaaaagcagg atatggctat
    56821 ctattgcctt acttaaacaa ataaaacaga gagggtgggg tttcccactt tcttcatttc
    56881 tagaatatga atagaaggga tttatgaaac acctattatc ttacttcaaa ccctacatca
    56941 aggaatcaat tttagccccc ttgttcaagc tgttagaagc tgtttttgag ctcttggttc
    57001 ccatggtgat tgctgggatt gttgaccaat ctttacctca gggagatcaa ggtcatctct
    57061 ggatgcagat tggcctgctc cttatctttg cagtaattgg cgttttagtg gccttgatag
    57121 ctcaatttta ctcagcaaag gcagcagtag gttttgctaa ggaattgaca aacgatcttt
    57181 atcgtcatat tctttccttg cccaaggaca gcagagaccg tctgacaact tctagtttgg
    57241 tcactcgctt gacttcggat acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc
    57301 tctttttacg agcgcccatt atcgtctttg gtgccatttt tatggcttat cgaatctcag
    57361 ctgagttgac tttctggttc ttagtcatgg ttgccatttt gaccattgtc attgtagggt
    57421 tatctcgatt ggtcaatcct ctctacagta gtctcagaaa gaaaacggac caactggttc
    57481 aggaaacgcg ccagcaattg caagggatgc gggttattcg tgcttttggt caagaaaaac
    57541 gagagttaca gatttttcaa acccttaacc aagtttatgc tagattacaa gaaaagacag
    57601 gtttctggtc tagtttatta acacctctga cctatctgat tgtcaatgga actcttctcg
    57661 ttattatctg gcaaggctat atttcaattc aaggaggagt gctcagtcaa ggtgctctca
    57721 ttgctcttat caattacctc ttacagattt tggtggaatt ggtcaagcta gccatgttga
    57781 tcaattccct caaccagtcc tatatctcag ccaagcgaat cgaggaagtc tttgttgagg
    57841 ctccagagga tatccattca gagttagaac aaaagcaagc taccagagat aaggttttac
    57901 aagtccaaga attgaccttt acctatcctg atgcagccca gccttctctg agagacattt
    57961 cctttgatat gactcaagga caaattctag gtatcatcgg gggaactggt tctggtaaat
    58021 caagcttgat tcaactcata cttggacttt atccagtaaa caagggaaga attgaccttt
    58081 atctagatgg attgagtcct cttaatttgg agcaatggag atcttggatt gcctatgtgc
    58141 cacaaaaggt cgaactcttt aagggaacta ttcgttccaa cttgacttta ggtttaaatc
    58201 aagaagtgac tgatcaagaa ctctggcaag ccttggaaat tgctcaagct aaggattttg
    58261 tcagtgataa ggaaggactc ttggatgctc tagttgaagc aggagggcga aatttctcag
    58321 gtggacaaaa acaaaggctg tctatcgcac gagcagtctt gcgccaggct ccgtttctca
    58381 tcctagatga tgcgacctca gcactggaca ccattacaga gtccaagctc ttgaaagcta
    58441 ttagagaaaa ttttccaaac atgagcttaa ttttgatctc tcaacgaacc tcgactttac
    58501 agatggcgga ccagattctc ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg
    58561 atgacttgat gaaatccagt caagtctatc gtgaaatcaa tgcatcccaa catggaaagg
    58621 aggactagaa tgaaacgaca aactgtaaac cagacgctca aacgtttagt cgtagattta
    58681 gcaaaccatc ccttcctcct tttcctagcc tttctaggaa ctattgccca agttggctta
    58741 tcaatttacc tacctattct gattgggcag gtcattgacc aagtcctagt ggctggttca
    58801 tcactagttt tttggcagat ttttctccag atgctcttgg tggtaatagg aaatactctg
    58861 gtacaatggg ccaatcctct cctctataat cgtctaatct tctcttatac cagagattta
    58921 cgggagcgaa tcatccataa gctccatcgt ttaccgattg cctttgtaga taggcaaggt
    58981 agtggagaga tggttagtcg tgtaaccacg gacatcgaac agttggcagc tggcttgacc
    59041 atgattttta accaattttt cattggtgtt ttgatgattt tggttagtat tctagccatg
    59101 ctccaaattc atctcctcat gactctctta gtcttgctgt tgacgccact gtccatggtg
    59161 atttcacgct ttattgccaa gagatcctat catctcttcc agaagcaaac agagacgagg
    59221 ggaattcaga ctcagttgat tgaagaatcg cttagccagc agactataat ccagtccttc
    59281 aatgctcaaa cagaatttat ccaaagattg cgtgaggctc atgacaacta ctcaggctat
    59341 tctcagtcag ccatctttta ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca
    59401 ctcatttatg cccttttagc tggagtagga gcttatcgta tcatgatggg ttcaaccttg
    59461 accgtcggtc gtttagtgac ttttttgaac tatgttcagc aatacaccaa gccctttaac
    59521 gatatttctt cagtgctagc tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat
    59581 ggagtcttag atagccctga agtggctgaa acaggtaagg aagtcttgac cagtgaccaa
    59641 gttaagggag ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg
    59701 attaaggact tgtctatcga tattccagct ggtagtaagg tagccatcgt tggtccgaca
    59761 ggtgctggaa aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga
    59821 gatatcttgc tggatgggca atccatttat gattatacac gagcatcatt gagacagcag
    59881 tttggcatgg tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc
    59941 tttggcaatc ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca
    60001 gactttttca tccaacagtt gccacaggga tacgatacca agttggaaaa tgctggagaa
    60061 tctctctctg tcggccaagc tcagctcttg accatagccc gagtctttct ggctattcca
    60121 aagattctta tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta
    60181 caggatgcct ttgtaaaact catgaagggc cgcacaagtt tcatcattgc tcaccgtttg
    60241 tcaaccattc aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat
    60301 ggtaaccatc aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg
    60361 gcttttagtt ctgaataagc cattctcttt tgaaagttta tggacgaaaa aagttgcctt
    60421 cgagtgactt ttttgttaca atagctagaa aaattgttca ctgtaatact caatgaaaat
    60481 caaagagcaa actaggaaac tagccgtagg ttgctcaaag cacagctttg aggttgcaga
    60541 tagaactgac gaagtcagta accacaccta cggcaaagtg aatctgaagt ggtttgaaga
    60601 gagtacaact tgtcttttag aaaaggagcc tataatgaaa gtctttcagc atgtaaatat
    60661 cgtgacttgt gatcaagatt tccatgttta tcttgatgga atcttagcag tcaaggattc
    60721 tcaaatcgtc tatgtcggtc aagataagcc agcgttttta gagcaagctg agcagattat
    60781 ggactatcag ggagcttgga ttatgcctgg tttggtcaat tgtcacaccc attctgcaat
    60841 gacaggtctg agagggatcc gagatgacag caatctccat gaatggctca atgactatat
    60901 ctggccagca gaatctgagt ttactcccga catgactacc aatgcggtca aagaagcctt
    60961 gacagagatg ctccagtcag gaacaacaac ctttaacgat atgtataatc ccagtggtgt
    61021 ggatatccag caaatttatc aggtggtgaa aacttccaag atgcgttgtt atttttctcc
    61081 gactctcttt tcttcagaga cagaaacaac tgctgagact ataagcagaa ctcgatccat
    61141 catagacgaa atcttaaaat ataaaaatcc aaatttcaag gttatggtag cacctcattc
    61201 tccgtatagc tgcagtagag acttgctgga agcgagtttg gaaatggcaa aagagctaaa
    61261 tattccgctc catgtccatg tggcggagac caaggaggag tcaggaatta tcctcaaacg
    61321 atacggcaaa cgcccccttg cttttcttga agaactgggt tatttagatc atccgtccgt
    61381 atttgctcac ggggtcgaat taaacgagag agaaattgaa cgcttggcat cttctcaagt
    61441 ggctatcgcc cacaatccta tcagtaacct caaactggca tcaggaattg ctccaattat
    61501 ccagctccaa aaagcgggag tagcagtcgg aattgcgact gactcggttg cttccaataa
    61561 caatctagat atgtttgagg aaggaaggac tgcagctctt cttcagaaga tgaaaagtgg
    61621 ggatgccagc cagtttccaa tcgaaacagc tctcaaggta ctgacaatcg aaggggctaa
    61681 ggcccttgga atggaaaatc agataggaag tctggaagtc ggcaagcaag cagattttct
    61741 ggtcattcaa ccacaaggga aaattcatct ccaacctcag aaaaatatgc tgtctcacct
    61801 ggtttatgca gttaaatcta atgatgtaga tgatgtttat atcgccggag aacaggttgt
    61861 taagcaaggt caagtcctga cagtagaact ttaaaagaaa aatcacgaaa aattttaaaa
    61921 aaagtttgca aaaatcttgc attctttttt tgactatgct atacttatat acggtttaaa
    61981 aaaactgcct aagacagtag gggagctcga ctcataaata tcctaccgag gacaagacgt
    62041 atcatgtaaa aagaagcgta ttgtactttc gtgtctaggt ttgggcgcgt ttttcttttt
    62101 gaaaaattcc ccaagcaaaa taattacgga ggtgaacaca ctaatgagtg aagcaattat
    62161 tgctaaaaaa gcggaactag ttgacgtagt agctgaaaaa atgaaagctg ctgcatctat
    62221 cgtcgttgta gacgctcgtg gtttgacagt tgagcaagat acagttcttc gtcgtgagct
    62281 tcgtggaagc gaagttgagt ataaagttat taaaaactca atcttgcgtc gtgcagctga
    62341 aaaagctggt cttgaagatc ttgcatctgt atttgttgga ccatctgcag tagcattttc
    62401 taatgaagat gttatcgcac cagcgaaaat cttgaacgac ttttctaaaa acgctgaagc
    62461 acttgaaatt aaaggtggtg caatcgaagg cgctgtcgca tctaaagaag agattcttgc
    62521 acttgcaact cttccaaacc gcgaaggact tctttctatg ctcctttctg tacttcaagc
    62581 gccagtgcgc aacgttgctc ttgcagtcaa agcggttgca gaaagcaaag aagacgcggc
    62641 ttaatcttaa gctacacagc gtagcctagc tacgaaaaaa actattataa aatttaaaac
    62701 ttatttggag gaaataacaa tggcattgaa cattgaaaac attattgctg aaattaaaga
    62761 agcttcaatc cttgaattga acgaccttgt aaaagctatc gaagaagaat ttggtgtaac
    62821 tgcagctgct cctgtagctg ttgctgcagc tgatgcagct gatgctggtg ctgctaaaga
    62881 ttcattcgac gttgaattga catctgcagg cgacaaaaaa gttggcgtta tcaaagttgt
    62941 acgtgaaatc actggtcttg gtcttaaaga agctaaagaa cttgttgacg gtgcaccagc
    63001 acttgttaaa gaaggcgttg caactgcaga agctgaagaa atcaaagcta aattggaaga
    63061 agctggagct tcagttactc ttaaataata gagtatcgca atttgattgc ggaatcccta
    63121 tttaccagtc tttgagagcg ataagcgatt gatagaaact gataaattga tttggtttcc
    63181 tgccaaaa
//
LOCUS       DAVMHG010000002        39945 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMHG010000002 DAVMHG010000000
VERSION     DAVMHG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.002"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..147
                     /locus_tag="VYR08_000065"
     tRNA            74..147
                     /locus_tag="VYR08_000065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Met,seq:cat)
     gene            151..223
                     /locus_tag="VYR08_000066"
     tRNA            151..223
                     /locus_tag="VYR08_000066"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Phe,seq:gaa)
     gene            236..316
                     /locus_tag="VYR08_000067"
     tRNA            236..316
                     /locus_tag="VYR08_000067"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Tyr,seq:gta)
     gene            321..391
                     /locus_tag="VYR08_000068"
     tRNA            321..391
                     /locus_tag="VYR08_000068"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Trp,seq:cca)
     gene            406..478
                     /locus_tag="VYR08_000069"
     tRNA            406..478
                     /locus_tag="VYR08_000069"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:His,seq:gtg)
     gene            489..560
                     /locus_tag="VYR08_000070"
     tRNA            489..560
                     /locus_tag="VYR08_000070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Gln,seq:ttg)
     gene            570..653
                     /locus_tag="VYR08_000071"
     tRNA            570..653
                     /locus_tag="VYR08_000071"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Leu,seq:caa)
     gene            760..2037
                     /locus_tag="VYR08_000072"
     CDS             760..2037
                     /locus_tag="VYR08_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV3465664.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2113..3597
                     /locus_tag="VYR08_000073"
     CDS             2113..3597
                     /locus_tag="VYR08_000073"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV3465665.1"
                     /translation="MTLVYQSTRDANNIVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKTREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3658..4938
                     /locus_tag="VYR08_000074"
     CDS             3658..4938
                     /locus_tag="VYR08_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3465666.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4942..5562
                     /locus_tag="VYR08_000075"
     CDS             4942..5562
                     /locus_tag="VYR08_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3465667.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5728..6840
                     /locus_tag="VYR08_000076"
     CDS             5728..6840
                     /locus_tag="VYR08_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3465668.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7163..7588
                     /locus_tag="VYR08_000077"
     CDS             7163..7588
                     /locus_tag="VYR08_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV3465669.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7599..7958
                     /locus_tag="VYR08_000078"
     CDS             7599..7958
                     /locus_tag="VYR08_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV3465670.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8065..8709
                     /gene="pcp"
                     /locus_tag="VYR08_000079"
     CDS             8065..8709
                     /gene="pcp"
                     /locus_tag="VYR08_000079"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3465671.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8737..9594)
                     /locus_tag="VYR08_000080"
     CDS             complement(8737..9594)
                     /locus_tag="VYR08_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV3465672.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9724..10866
                     /gene="tgt"
                     /locus_tag="VYR08_000081"
     CDS             9724..10866
                     /gene="tgt"
                     /locus_tag="VYR08_000081"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV3465673.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10964..12781
                     /locus_tag="VYR08_000082"
     CDS             10964..12781
                     /locus_tag="VYR08_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3465674.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12934..14085
                     /gene="nagA"
                     /locus_tag="VYR08_000083"
     CDS             12934..14085
                     /gene="nagA"
                     /locus_tag="VYR08_000083"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV3465675.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14248..15306
                     /locus_tag="VYR08_000084"
     CDS             14248..15306
                     /locus_tag="VYR08_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV3465676.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15457..15822
                     /locus_tag="VYR08_000085"
     CDS             15457..15822
                     /locus_tag="VYR08_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV3465677.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15897..16838
                     /locus_tag="VYR08_000086"
     CDS             15897..16838
                     /locus_tag="VYR08_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV3465678.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16786..17802
                     /locus_tag="VYR08_000087"
     CDS             16786..17802
                     /locus_tag="VYR08_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV3465679.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17804..18130
                     /locus_tag="VYR08_000088"
     CDS             17804..18130
                     /locus_tag="VYR08_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV3465680.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18099..18527
                     /locus_tag="VYR08_000089"
     CDS             18099..18527
                     /locus_tag="VYR08_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV3465681.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18532..18792
                     /locus_tag="VYR08_000090"
     CDS             18532..18792
                     /locus_tag="VYR08_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465682.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18755..19216
                     /locus_tag="VYR08_000091"
     CDS             18755..19216
                     /locus_tag="VYR08_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3465683.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIHRVEKEKS"
     gene            19194..19607
                     /locus_tag="VYR08_000092"
     CDS             19194..19607
                     /locus_tag="VYR08_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV3465684.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19744..20338
                     /locus_tag="VYR08_000093"
                     /pseudo
     CDS             19744..20338
                     /locus_tag="VYR08_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20399..21352
                     /locus_tag="VYR08_000094"
     CDS             20399..21352
                     /locus_tag="VYR08_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3465685.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21403..22593
                     /locus_tag="VYR08_000095"
     CDS             21403..22593
                     /locus_tag="VYR08_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV3465686.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22742..23113
                     /gene="rnpA"
                     /locus_tag="VYR08_000096"
     CDS             22742..23113
                     /gene="rnpA"
                     /locus_tag="VYR08_000096"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV3465687.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23088..23912
                     /locus_tag="VYR08_000097"
     CDS             23088..23912
                     /locus_tag="VYR08_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3465688.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23931..24917
                     /locus_tag="VYR08_000098"
     CDS             23931..24917
                     /locus_tag="VYR08_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV3465689.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24968..25591
                     /locus_tag="VYR08_000099"
     CDS             24968..25591
                     /locus_tag="VYR08_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV3465690.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25722..27179
                     /locus_tag="VYR08_000100"
     CDS             25722..27179
                     /locus_tag="VYR08_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3465691.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFWLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27202..27483
                     /locus_tag="VYR08_000101"
     CDS             27202..27483
                     /locus_tag="VYR08_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3465692.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27560..28045
                     /locus_tag="VYR08_000102"
     CDS             27560..28045
                     /locus_tag="VYR08_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3465693.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28062..28727
                     /locus_tag="VYR08_000103"
     CDS             28062..28727
                     /locus_tag="VYR08_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV3465694.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            28731..29594
                     /locus_tag="VYR08_000104"
     CDS             28731..29594
                     /locus_tag="VYR08_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV3465695.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKSCDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29596..30300
                     /locus_tag="VYR08_000105"
     CDS             29596..30300
                     /locus_tag="VYR08_000105"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV3465696.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30479..32152
                     /locus_tag="VYR08_000106"
     CDS             30479..32152
                     /locus_tag="VYR08_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3465697.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32264..33355
                     /gene="ulaG"
                     /locus_tag="VYR08_000107"
     CDS             32264..33355
                     /gene="ulaG"
                     /locus_tag="VYR08_000107"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV3465698.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33469..35445
                     /gene="tkt"
                     /locus_tag="VYR08_000108"
     CDS             33469..35445
                     /gene="tkt"
                     /locus_tag="VYR08_000108"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3465699.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEEGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGENIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35562..35861
                     /gene="yajC"
                     /locus_tag="VYR08_000109"
     CDS             35562..35861
                     /gene="yajC"
                     /locus_tag="VYR08_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3465700.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35907..36335
                     /locus_tag="VYR08_000110"
     CDS             35907..36335
                     /locus_tag="VYR08_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV3465701.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36337..36747
                     /locus_tag="VYR08_000111"
     CDS             36337..36747
                     /locus_tag="VYR08_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465702.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37047..39698
                     /gene="adhE"
                     /locus_tag="VYR08_000112"
                     /gene_synonym="adhC"
     CDS             37047..39698
                     /gene="adhE"
                     /locus_tag="VYR08_000112"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV3465703.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLNQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12130 a         7151 c         9009 g        11655 t
ORIGIN      
        1 gaacggtctt gaaaaccgtc aggcgtgtaa aagcgtgcgt gggttcgaat cccacatcct
       61 ccttttatat taacgcggga tggagcagct cggtagctcg tcgggctcat aacccgaagg
      121 tcgtaggttc aaatcctgct cccgcaataa ggctcggtag ctcagttggt agagcaatgg
      181 attgaagctc catgtgtcgg cggttcgatt ccgtctcgcg ccatttatat attttggaag
      241 ggtagcgaag aggctaaacg cggcggactg taaatccgct ccttcgggtt cgggggttcg
      301 aatccctccc cttccatttt acgggcatag tttaaaggta gaactaaggt ctccaaaacc
      361 ttcagtgtgg gttcaattcc tactgcccgt gttaatagaa ttatggcggg tgtggtgaag
      421 tggttaacac accagattgt ggctctggca tgcgtgggtt cgatccccat cactcgccta
      481 ttttatattg gggtatagcc aagcggtaag gcaagggact ttgactccct catgcgttgg
      541 ttcgaatcca gctaccccag ttactatttg ccggcgtggc ggaattggca gacgcgctgg
      601 actcaaaatc cagtgtccgc aaggacgtgc cggttcgacc ccggccgccg gtatagtata
      661 gtgttaggaa cgttgttatt cttcgttcct tttttatatt atttttggta taattatagt
      721 tattcaaatt ttatttagat taagaaagtg taggggagta tgtcttgttc tatcgattta
      781 ttaaaacatc ggtatttgaa aaatattaaa gaaaatcctg aattgtttgt cggaattgag
      841 ttggagtatc ctgttgcaag tttagaaggg gatgctacag atgttgaagt tatgaaggat
      901 ctatttcatt atttagtttc tactttggat ctcaccgtag caaaggtaga tgattttggc
      961 aatctgatcc agttagtaga tccgataagt caggatgcta ttttatttga agtttcctat
     1021 acaacgattg agtttgcatt tggtaaggct gaaacgattc aagaggtcga aaatcgtttc
     1081 aataattata tgaatgtaat tcagagaaag ttagctgaat caaatcatgc tattgttggc
     1141 tgtggtatcc atcccaactg ggataaaaat gagaattgtc cagtggctta tccacgctat
     1201 cagatgttga tggattattt gaatttgagt agaaatatta ttaaatcaga tttacatcat
     1261 ttccctgaat atggtacttt tatctgtggg agccaggttc agctggatat ttcaaaaacc
     1321 aactacttac gggtgattaa tgcttttact caaattgaag cggctaaggc ttatttattt
     1381 gcaaactctg aattttcggg tgcggattgg gatacgaaaa tttcaaggga tattttctgg
     1441 gaagaatcta tgcatggtat ctatccagag aatgttgggg tcaatgctag actccttaat
     1501 gatgaagctg atttttttga ctatctaaat cattctgcga tttttactgc ggaacgtgat
     1561 gggcagacct attattttta tcctattcag gctggggact atttggctac gtccgaaatc
     1621 caagcatttg ctctgaatgg ggatgaggtt attatttacc cccaagagaa ggattttgaa
     1681 actcatcgta gttaccagta ccaagattta acgactcgag gaacagttga gtttcgtagt
     1741 gtgtgtacac agccacttga taggactttt gcttctgcag cttttcactt gggattattg
     1801 gttaatttag acaagttaga agcttactta gaaacagcac ctttctttaa agtatttggt
     1861 tatgattaca agtctttaag gagacaattt tctaagaaaa atcttacaga tgaggaagaa
     1921 actacgatta ttgaattttc caaagactta ctcctactag ctgaggaggg actagtggtg
     1981 agaaataagg aagaaatgac ctatttacag cctttgagag aagaattgag cctataattt
     2041 ctcttataaa gggagaattt tctgaaaaat catgatataa tggacgagac tatagataaa
     2101 ggatagagag taatgacatt agtttatcaa tcaacgcgtg atgccaacaa tatagtaact
     2161 gccagccaag caattttgca aggtttggcg acggacggcg gtttgtttac accggttact
     2221 tatccaaagg tagatttgga ctttgacaaa ttgaaagatg cttcttacca ggaagttgct
     2281 aagctagttt tgtcagcatt tttagatgac tttacagctg aggagttgga ctactgtatc
     2341 aacaatgcct acgatagcaa atttgatact ccagctattg caccattagt gaaattagat
     2401 gggcaataca atttggaact tttccatggt tcaacgattg cctttaagga tatggccttg
     2461 tctattttgc catactttat gacgactgct gctaagaaac atggtttgga gaacaagatt
     2521 gttatcttga cagcgacatc tggtgacacg gggaaagctg ctatggcggg gtttgcggat
     2581 gtgcctggta ctgagattat cgtcttttat ccaaaggatg gtgtcagcaa gattcaagag
     2641 ttacaaatga ccactcaaac tggcgacaat actcatgtta ttgctattga tggtaacttt
     2701 gacgatgcgc aaacaaatgt gaagcacatg tttaacgacg tggctcttcg tgaaaaattg
     2761 actaccaaca agttgcaatt ttcatcagct aactctatga acattggtcg tctggtgcca
     2821 caaattgttt attatgttta tgcttacgct caattggtca agactggtga gatcgtggct
     2881 ggtgataaga ttaacttcac agtaccaaca ggaaactttg gaaatatctt ggctgccttt
     2941 tatgccaaac aaatcggtct cccagttggt aaattgatct gtgcttcaaa tgacaacaat
     3001 gttttgacag acttcttcaa gacacgtgtc tatgacaaga cacgtgagtt taaggtaaca
     3061 accagcccat ctatggatat cttggtatct tcaaacttgg agcgtttgat tttccatctt
     3121 ttggggaatg atgcggttaa gacggctgaa ctcatgaatg ctttgaacac gcaaggacaa
     3181 tataagttga cagactttga tgcagagatt ttggacctct ttgcagctga atatgcgact
     3241 gaggaagaaa cggcagcaga gatcaagcgt gtttgtgagt tagattctta tatcgaggac
     3301 cctcatacag ctgttgcttc agcagtttat aaaaaatacc aatcggccac tggagatgta
     3361 actaagacag tgattgcttc aacagctagt ccatacaagt tcccagtagt tgcagtagaa
     3421 gctgtaactg gaaaagcagg tttaacagac tttgaagcct tggctcaatt acatgaaatc
     3481 tcaggcgttg cagtgccacc agcagttgat gggcttgaaa tagctccaat tcgtcacaag
     3541 acaacagtgg cagctgctga catgcaagca gcggttgagg cttatttagg actttaagac
     3601 agagggagca aactcggttg ggaaaccaac tgagtttctt ttcatcagga ggagagattg
     3661 tttaagaaaa ataaagacat tcttaatatt gcattgccag ctatgggtga aaactttttg
     3721 cagatgctaa tgggaatggt ggacagttat ttggttgctc atttaggatt gatagctatt
     3781 tcaggggttt cagtagctgg taatattatc accatttatc aggcgatttt catcgctctg
     3841 ggagctgcta tttccagtgt tatttcaaaa agcatagggc agaaagacca gtcgaagttg
     3901 gcctatcatg tgactgaggc gttgaagatt accttactat taagtttcct tttaggattt
     3961 ttgtccatct tcgctgggaa agagatgata ggacttttgg ggacggagag ggatgtagct
     4021 gagagtggtg gactgtatct atctttggta ggcggatcga ttgttctctt aggtttaatg
     4081 actagtctag gagccttgat tcgtgcaacg cataatccac gtctgcctct ctatgttagt
     4141 tttttatcca atgccttgaa tattcttttt tcaagtctag ctatttttgt tctggatatg
     4201 gggatagctg gtgttgcttg ggggacaatt gtgtctcgtt tggttggtct tgtgattttg
     4261 tggtcacaat taaaactgcc ttatgggaag ccaacttttg gtttagataa ggaactgttg
     4321 accttggctt taccagcagc tggagagcga cttatgatga gggctggaga tgtagtgatc
     4381 attgccttgg tcgtttcttt tgggacggag gcagttgctg ggaatgcaat cggagaagtc
     4441 ttgacccagt ttaactatat gcctgccttt ggcgtcgcta cggcaacggt catgctgttg
     4501 gcccgagcag ttggagagga tgattggaaa agagttgcta gtttgagtaa acaaaccttt
     4561 tggctttctc tgttcctcat gttgcccctg tcctttagta tatatgtctt gggtgtacca
     4621 ttaactcatc tctatacgac tgattctcta gcggtggagg ctagtgttct agtgacactg
     4681 ttttcactac ttgggacccc tatgacgaca ggaacagtca tctatacggc agtctggcag
     4741 ggattaggaa atgcacgcct ccctttttat gcgacaagta taggaatgtg gtgtatccgc
     4801 attgggacag gatatctgat ggggattgtg cttggttggg gcttgcctgg tatttgggca
     4861 gggtctctct tggataatgg ttttcgctgg ttatttctac gctatcgtta ccagcgctat
     4921 atgagcttga aaggatagga aatgcaaaaa acagctttta tttgggattt agacgggact
     4981 ttattggact cttacgaagc gattttatca gggattgagg agacttttgc tcagttttct
     5041 attccttatg ataaggagaa ggtgagagag tttatcttca agtattcggt gcaagatttg
     5101 cttgtgcggg tggcagaaga tagaaatctg gatgttgagg tgctaaatca ggtgcgtgcc
     5161 cagagtctgg ctgagaagaa tgctcaggta gttttgatgc caggtgcgcg tgaggtgcta
     5221 gcttgggcag acgaatcagg aattcagcag tttatatata ctcataaggg gaacaacgct
     5281 tttaccattc tcaaggactt gggggtggaa tcctatttta cagagatttt aaccagtcag
     5341 agtggctttg tgcggaagcc aagtccagaa gcggctacct atctgctaga taagtatcag
     5401 ttgaattctg ataatactta ttatataggg gatcggactc tggatgtgga atttgcccag
     5461 aatagtggga ttcaaagcat caacttttta gagtctactt atgaagggaa tcacaggatt
     5521 caagcgttag cagatatttc ccgtattttt gagactaagt gataaaaaga ttgtgtcagt
     5581 tttgtgacag agacctaaca aactatttca agtaacctag tttgttacaa ggaatagaca
     5641 gttctgttaa ataggcccga gagggctttt tttctacatt ttttgtgtta tgatagacag
     5701 gtactcattt gaaaggaatt tgaaagaatg aagaaaagaa tgttattagc gtcaacagta
     5761 gccttgtcat ttgccccagt attggcaact caagcagaag aagttctttg gactgcacgt
     5821 agtgttgagc aaatccaaaa cgatttgact aaaacggaca acaaaacaag ttataccgta
     5881 cagtatggtg atactttgag caccattgca gaagccttgg gtgtagatgt aacagtgctt
     5941 gcgaatctga acaaaatcac taatatggac ttgattttcc cagaaactgt tttgacaacg
     6001 actgtcaatg aagcagaaga agtaacagaa gttgaaatcc aaacacctca agcagactct
     6061 agtgaagaag tgacaactgc gacagcagat ttgaccacta atcaagtgac cgttgatgat
     6121 caaactgttc aggttgcaga cctttctcaa ccaattgcag aagttacaaa gacagtgatt
     6181 gcttctgaag aagtggcacc atctacgggc acttctgtcc cagaggagca aacgaccgaa
     6241 acaactcgcc cagttgaaga agcaactcct caggaaacga ctccagctga gaagcaggaa
     6301 acacaagcaa gccctcaagc tgcatcagca gtggaagtaa ctacaacaag ttcagaagca
     6361 aaagaagtag catcatcaaa tggagctaca gcagcagttt ctacttatca accagaagag
     6421 acgaaaataa tttcaacaac ttacgaggct ccagctgcgc ccgattatgc tggacttgca
     6481 gtagcaaaat ctgaaaatgc aggtcttcaa ccacaaacag ctgcctttaa agaagaaatt
     6541 gctaacttgt ttggcattac atcctttagt ggttatcgtc caggagacag tggagatcac
     6601 ggaaaaggtt tggctatcga ctttatggta ccagaacgtt cagaattagg ggataagatt
     6661 gcggaatatg ctattcaaaa tatggccagc cgtggcatta gttacatcat ctggaaacaa
     6721 cgtttctatg ctccattcga tagcaaatat gggccagcta acacttggaa cccaatgcca
     6781 gaccgtggta gtgtgacaga aaatcactat gatcacgttc acgtttcaat gaatggataa
     6841 acccgacttg ataacatcat tttgacgaat gagatctagc tttcgtgatg gaaagcgatt
     6901 ctcgttcgtt ttttctttgt catactcttc gaaaatctct tcaaaccacg tcagttttat
     6961 ctgaaacttc aaagctgtgc tttgagcaac ctgcgactag cttcctagtt tgctttttga
     7021 ttttcattga gtatcaattt gaatggaaaa tggaaagtta tcatcttgta atgagttaag
     7081 caacattctt gcaatctatt ttactttata tcacaattaa ttagtcaaat attgataaat
     7141 caataaaaag agaggggaag aaatgctaga gattcaagat ttactgtatc aactccgctt
     7201 gtctgagcaa gcgagtacgc aattgtttga aaaaaggctt gggattagtt tgacacggta
     7261 tcagatttta ctgtttttgc tggagcattc tccttgtaac caaatggcgg ttcaggagcg
     7321 tttgaaaatt gatcaggctg ctttgacacg gcatttcaaa attttggaaa cggaaggttt
     7381 ggtggagcgt catcgtaatc ctgaaaatca gcgggaagtg ttggtagagg ctgcgaagta
     7441 tgccaaggag cagttagtgg tgaatccccc tctgcaacat atcagggtta aggaagagat
     7501 agaaagtatc ttaacagagt ttgagagaac agaactcagc cgtttattaa ataaattggt
     7561 tttgggtatt gaaaatatag aaatttaagg agaaatagat gtcaattatt ttaacaacga
     7621 tcgttgcttt ggagcatttt tacatttttt atttggaaag tattgccacg caatcagatg
     7681 cgactagtcg tgtatttaat atggaaaagg aagaattggc tcatccgtca gtaagttcat
     7741 tgttcaaaaa tcaaggaatt tataaggctc tgctaggagt ctttctcttg tatgtcattt
     7801 atttctcaca gaatttagaa attgtgacta tttttgtctt atttgtgatt ggtgctgcga
     7861 cttacggctc tttaacagcg gataaaaaaa ttattttgaa acaaggtgga tcagctattt
     7921 tggccttgat tagtatttta ctctttaaat acacttgaag gtcgattcta atctcgctaa
     7981 tcctttttaa tccagaataa gggaaatatg ttatacttgt ttttaagaaa aaagtctcat
     8041 tgaattggtt ttgaggagtt agaaatgaaa gtattagtga caggttttga gccctttgga
     8101 ggggaaaagg gcaatccagc tttggaggcc attaaaggtt taccagctga aatccatggt
     8161 gctgaggtcc gttggctaga ggtgccgaca gtttttcaca aatctgctca agtattggaa
     8221 gaagagatga atcgttatca acctgacttt gtcctttgta ttgggcaagc tggtggaaga
     8281 actagtttga cacctgaacg agtgaccatt aatcaagacg atgcatgcat ttctgataac
     8341 gaagataatc aaccgattga ccgtcccatt cgcccagatg gtgcttcggc ctactttagt
     8401 agtttgccga ttaaagcgat ggttcaagct ataaaaaaag agggcttacc ggcctctgtt
     8461 tccaatacgg cagggacttt tgtctgcagc catttgatgt atcaggctct ctatttggta
     8521 gaaaagaaat ctccatatgt taaggcaggt tttatgcata ttccttatat gatggaacag
     8581 gtggtgaaca gaccgactac tccagctatg agtttagtgg atattcggcg agggatagaa
     8641 gcagcaatcg gcgctataat agaacatgga gatcaggaac tcaagttggt aggcggagaa
     8701 actcattgat agaaaaaagc ttgaggggaa aaaccttcaa gcttttggac gttttcgggc
     8761 caatactgct cggtaaaaca taattttagt gcattggata taaggtagga gtgaaaaact
     8821 agcaatgcca aaggtaatcc aattgaggaa gtaccaagga agaagctgta aatctaggac
     8881 aaagtgctgg aacttgtagc ccttcataaa ggaacggcta gtttttagga ttcgtcttgg
     8941 tgggacctgt cctaggtcta gactataaca gagaagaaat tccacctgtg aataggcata
     9001 atactgtgga atatagagga tatttcctac aatgatcaag atgagacttg caagaaagta
     9061 gagtccaaag accatgagga aacgctcggt ttcaactgat gagagatcta gatttggaaa
     9121 ctcaggatgt agggtgacga attttttggc taaaaagcta ctataaaaga ggaggtaaat
     9181 cccaagtaaa ttagggatac tccataaaaa gagatagaaa cgtttgagaa gtagggtcaa
     9241 aaaggtttga gaaaagcgct cctcatcaaa gagagctagg ctgtttttta cagatggctc
     9301 cgttttagaa tctttcatga gtgtcagtgt tgcatagacg gaactggtca aaagaatagt
     9361 cccgataaag gagactagta gaggaaagag gtaggtttga agtatttggc caagtatgct
     9421 gaaaaatggc tgttctaaaa cagtcccgtg gatccgagat aagggattaa gaaaaccaga
     9481 taagatgacc agcatactgg gaaggatata gaggagaaag agacgggggg tgtcagcctg
     9541 aaaatgtttt gactcctgac gaattgtttt taaatcaatt tttggatagt tcattctctt
     9601 attataccat agttcttata catagttcgt gacagttcct actttttttg ataaaatcat
     9661 acagtgtgtc cttgggcaca ctgtatgaac tgggactgtc tttcccagct tcggaggtaa
     9721 aaaatgtcag attcaccaat caaatatcgt ttgattaaga aagaaaaaca cacaggagct
     9781 cgtctgggag aaatcatcac tccccacggt acctttccga cacctatgtt tatgccagtt
     9841 gggacacaag ccactgtcaa aactcagtca cctgaagaat tgaaggagat gggttcggga
     9901 attatcctat caaataccta tcatctctgg cttcgccctg gagatgaact cattgcacgc
     9961 gctggtggtc tccacaagtt catgaattgg gaccagccta tcttgacaga tagtggtggt
    10021 tttcaggttt attctttagc agatagccgt aatatcacag aagaaggagt aacctttaaa
    10081 aatcatctaa atggttctaa gatgttccta tccccagaaa aagccatctc tattcagaat
    10141 aatctgggtt cagacatcat gatgtccttt gatgaatgtc ctcagtttta tcaaccttat
    10201 gactacgtta agaaatcgat cgagcgtacc agccgttggg ctgagcgtgg tttgaaggct
    10261 caccgtcgtc cacatgacca aggtttgttt ggaattgtgc aaggtgcagg atttgaagac
    10321 cttcgccgcc aatcagctca tgatcttgtc agcatggatt tctcaggcta ctctatcggt
    10381 ggtttggcag tgggagaaac ccatgaagag atgaatgcgg tcttggactt tacaactcaa
    10441 ctgctacctg aaaataaacc tcgttatctg atgggtgtgg gagcgccaga tagcttgatc
    10501 gatggggtca ttcgtggggt ggatatgttt gactgtgtct taccgactcg aattgctcgt
    10561 aacgggactt gtatgaccag tcaaggacgt ttggttgtga aaaatgccca gtttgctgag
    10621 gactttacgc cactggatcc tgagtgtgac tgctacacat gtaataacta tacacgcgct
    10681 taccttcgtc acctgctcaa ggctgatgaa acctttggta tccgcttgac tagctaccac
    10741 aatctttact tcttgcttaa cctgatgaag caagtgcgac aagccatcat ggatgacaat
    10801 ctcttggaat tccgtgagta ttttgtggaa aaatatggct ataataagtc aggacgtaat
    10861 ttctaaaatg gaattgatat aaaaaaatcc taagttttct cttaggattt ttcttctttt
    10921 tttgatagaa taaagtgtac aatgaaagga agaataaact cgtatgcgca ttaaatggtt
    10981 ttccttgatt aggattatag gtttactttt ggtactcttg tatcacttct ttcagacgat
    11041 ctttcctgga ggatttttcg gggtagatgt ctttttcaca ttttcaggtt tcctgattac
    11101 agctctactc attgaagaat tttctaagaa caatgagatt gatttgatag gattttttag
    11161 aagacgcttt tatcggattg tgccacctgt ggttttgatg gtcttggtga ccatgccttt
    11221 tactttcttg gttcgccaag actatgttgc tggaattggt ggccagattg cgggcgtctt
    11281 aggctttatg accaacttct atgaactcct aacaggtggg agttatgaat ctcagttcat
    11341 tcctcatttg tttgttcata attggagttt ggcagttgag gttcactact atattctttg
    11401 gggattggca gtttggttct tatccaaaca agctaaatca aatggtcagt tgaaggggat
    11461 ggtctttctc ttatctgctg ctgccttctt gatcagtttc ttctccatgt ttattggtag
    11521 ttttctagtg acctcttatt cctctgttta tttctccagt ttaactcatg tctatccatt
    11581 ctttttggga agtatgttag caactattgt aggtgttcgt cagacgactt ccctcgtcaa
    11641 gcagttggat aaaatctggg atttacgaaa gactttggta gtttttggag gaggctttgg
    11701 tttcctagtt cttttgactt tctttgtcaa attcacttat ctttttgcct atcttatcgg
    11761 cttcttgctt gccagtcttg cagctcttgc catgattctg gcggcgcgtg tcttacatga
    11821 aaagacacat catatacagg agccgaagat tatcagcttt ttagcggata ctagctatgc
    11881 ggtttatctt ttccattggc ctttctatat cattttctca cagttgacat caaatcttct
    11941 tgctgtatta ctgactctga tttgttctta tggctttgcc agtctgtcat tctatgtatt
    12001 ggaaccttgg attgcaggca agaacacacc tattttacaa actcttcgtc ccctgcctta
    12061 tattcacgca attcttgtag caggtacagg aatcttgacc atcattgtct gcacggtgac
    12121 cttgttggca ccacaagtgg gagcgtttga gacagacttg actgtcaatg gcttgaagca
    12181 agctgcaaca aatattggcc agaccaaggt gatggcagaa cgggcagatg caaacagttt
    12241 gggaattgct gatggcacta tgttaattgg tgactcagtg gctttaaggg caaatacagc
    12301 actacagaca gctcttcctg gagcacagat taacgcgcag gtcagcgtaa caaccaagac
    12361 cgcaaatgaa atcatgctaa ataatagcca gaataaattt ttacctaaga cggtggtcat
    12421 tgcgactggg gtaaataatc ctgagaatta caaggatgac tgggacagta tcgtgaaaaa
    12481 tcttcctaag ggacaccata tgattttggt gactccttat gagggagata agacaaaaga
    12541 gacctatgcc atcgttgaga aggctgctgc ctatatgaga gaattggcag agaagacacc
    12601 ttacattacg atagcagatt ggaatcaagt tgcgaaagag catccagaaa tttgggctgg
    12661 aacagaccag gttcatttcg ggagtgagag tagcactatc gaagcaggag caaaattgta
    12721 tgcagatacg attgccacag ctttgcagac agctcaagac aagccggtta aatcaaaata
    12781 acttgtatta aaaaagagaa gagttggaga aatccagctc ttttaaaata tctccaaaaa
    12841 ataggtctat accatttaca aataaaaaag aaaggtttat aatgtaattg acataataaa
    12901 ttgtagaatc aatcttttaa ggaggttaac attatgccta actatattaa agcggatcag
    12961 tttttctacc cacacggagt tcgtcgaggt ggttacttgg aacttgtgga cggcaagttt
    13021 gggaaacatg tagagcagat tcctgaaggg gctgaggtga ttgactatac aggttatagc
    13081 attgccccag gtcttgtgga tactcatatt catggatatg caggtgtaga tgtgatggac
    13141 aacaacattg aaggtacatt gcatactatg agtgaaggac ttcttagtac cggtgttacc
    13201 agtttcttac ccacaacttt aacagccact tatgagcaat tgcttgcagt cactgaaaat
    13261 cttggaaacc attataaaga agcaacaggt gctaagattc gtgggattta ttatgaaggt
    13321 ccatatttca cagaaacttt taagggggca caaaatccaa cttatatgag agacccgggt
    13381 gttgaggagt ttcattcttg gcaaaaagcg gcaaatggct tgcttaataa aattgccctt
    13441 gcaccagaac gtgatggggt ggaagacttt gttcgtacag ttacgggcga aggtgtgacg
    13501 gttgctcttg gacattcaaa cgcgactttt gatgaagcca aaaaagcagt cgatgctgga
    13561 gcgagtgttt gggtgcatgc ctacaatgga atgcgtgggt tgactcaccg tgaattgggt
    13621 atggttggag ccatgtacca attgccacat acctatgcag agttgatctg tgatggtcac
    13681 cacgtagatc caaaggcttg cgaaattctt atcaaacaaa aaggaacaga aaacatcgct
    13741 cttatcacag actgtatgac agctggggga ttggaagacg gagattatat gttgggagaa
    13801 ttcccagttg tcgttgcaaa tggaactgca cgcctcaaat cgacaggtaa cttggcaggt
    13861 tctatcctca aactcaaaga tggtttgaag aatgtggtcg aatggggaat tgcgaatccg
    13921 catgaagcag tcatgatggc cagcttcaac ccagctaaat ccgttcacat cgatgacgtc
    13981 tgtggccaaa tccgtgaagg ctacgacgct gacttcatcg tattagataa agatttggaa
    14041 ttggtagcaa cctatctaga tggcgtaaaa cgttatcaag cataagagag aaggcagaag
    14101 ttagagacta gcttctgctt ttttatacag ggtactttac tggtaaataa aaagttaaaa
    14161 aaatcacaaa aaaagcttga agaaacaaag aaaaaggctt acaattgaac tataaatatt
    14221 acaaataaaa aaacggagga gtgctttatg aaagcctata cttatgttaa accaggactt
    14281 gcttcttttg ttgatgtaga caaaccagtt attcgcaagc caacagacgc tattgtgcgt
    14341 attgtaaaaa ccactatttg tggaacagac ctccatatta tcaaagggga tgttcctact
    14401 tgccaaagtg gtaccattct tggccacgaa gggattggga ttgttgaaga agttggggaa
    14461 ggagtttcca acttcaaaaa aggcgacaag gtcttgattt cttgcgtctg tgcctgtggt
    14521 aaatgctact actgtaaaaa aggaatttat gcccactgtg aagacgaagg gggctggatt
    14581 ttcggtcact tgattgatgg tatgcaggct gaatatctac gtgtccctca tgcagataat
    14641 actctttacc atactccaga agacttgtca gatgaagctt tggttatgct gtcagacatt
    14701 ctgcctactg gatatgaaat tggtgtctta aaagggaaag tagaacctgg ttgcagcgta
    14761 gccattattg gttcaggtcc agttggattg gctgctcttt taacagccca attctattca
    14821 ccagctaaat tgattatggt agacctagac gataaccgct tggaaactgc cctatcattc
    14881 ggtgcgactc ataaggttaa ttcttcagac cctaaaaaag ccattaaaga aatttatgat
    14941 ttgacagatg gtcgtggtgt ggatgtcgct atcgaagctg tagggattcc tgaaacattt
    15001 gatttctgtc aaaagattat cggtgtagac ggaacggttg ccaactgtgg tgtgcatggt
    15061 aaaccagttg aattcgattt agataaactt tggattcgca acatcaatgt aacaactggt
    15121 ttggtatcta caaatacgac tccacaattg ttgaaagcac ttgaaagtca taagattgaa
    15181 ccggaaaaat tggtaactca ctatttcaaa ctcagtgaaa ttgaaaaagc ctacgaagtc
    15241 ttcagtaagg cagcagacca ccatgccatt aaggtcatta tcgaaaacga tatctcagaa
    15301 gcctaagtag taaaaatatt tttgtacata agtaaataga aattcagtca tccatcagat
    15361 ggttggattt tttatcaaaa aattaagaaa tgagcatatt tctttccttg tctggcggaa
    15421 ttggttataa tatacggtac aaaggaatga atgaatatgt atcgtgttat agaaatgtac
    15481 ggagattttg aaccgtggtg gttcttagaa ggttgggaag aagatattgt agcaagtaga
    15541 aaatttgacc agtattatga tgctctcaaa tactacaaaa cttgctggtt tagattggaa
    15601 caagaatcgc ctctttataa aagtagaagc gacttgatga ccattttttg ggacccggaa
    15661 gaccaacgct ggtgtgatga atgtgatgag tatttacaac aataccattc tttggctctt
    15721 ttgcaggatg agcaggttat cccagacgaa aaactacgct caggctatga aaaacaaacc
    15781 agtcaggaaa ggaatcgttc ttgccgtatg aaattaaaat agagaaaagt aactttttgg
    15841 agttgctttt tttatttttc taacgctttg cgaatagtat aggtgaggag gtaagtatgg
    15901 ttcaagaaat tgcacaagaa atcattcgtt cggctcggaa aaaaggggcg caagacattt
    15961 attttgtccc caagttagat gcctatgagc ttcatatgag ggtaggagac gagcgctgta
    16021 aaattggtag ctatgatttt gaaaagtttg cagccgttat cagtcacttt aagtttgtgg
    16081 cgggtatgaa tgtgggagaa aaaagacgta gtcaactggg ttcctgtgat tatgcctatg
    16141 accagaagat ggcgtctcta cgtttatcta ctgtaggcga ttatcggggg catgagagtt
    16201 tggttatccg tttgttgcac gatgaggagc aggacttgca tttttggttt caggatattg
    16261 aagaattagg caagcagtac aggcaacggg gactctatct ttttgctggt ccggttggga
    16321 gtggtaagac gaccttgatg catgaattgt ccaagtcact ctttaaagga cagcaagtta
    16381 tgtccatcga agatcctgtc gaaatcaagc aggacgacat gcttcagttg cagttgaacg
    16441 aagcaatcgg cctaacctat gaaaatctaa tcaaactttc cttgcgtcat cgaccagatc
    16501 tcttgattat cggagaaatt cgtgacagcg agacggcgcg tgcagtggtc agagctagtt
    16561 tgacaggtgc gacagtcttt tcaaccattc acgccaagag tatccgaggt gtttatgagc
    16621 gtctgctgga gttgggtgtg agtgaagaag aattggcagt tgttctgcaa ggagtctgct
    16681 accagagatt aatcggggga ggaggaatcg ttgactttgc aaacagagat tatcaagaac
    16741 accaagcagc caagtggaat gagcaaattg accagcttct taaagatgga catatcacaa
    16801 gtcttcaggc tgagacggaa aaaattagct acagctaagc aaaaaaatat catcacccta
    16861 tttaacaatc tcttttctag cggttttcat ctggtggaga ctatctcctt tttagatagg
    16921 agttccttgt tggacaagca gtgtgtgacc cagatgcgta caggcttgtc tcaggggaaa
    16981 tcattctcag aaatgatgga aagtttggga tgttcaagta ccattgtcac tcagttatct
    17041 ctagctgaag ttcatggcaa tcttcacctg agtttgggaa agatagaaga atatctagac
    17101 aatctggcca aggtcaagaa aaagttaatt gaagtagcga cctatccttt gattttgctg
    17161 ggttttcttc tcttaattat gctggggcta cggaattacc tgctcccaca actggatagt
    17221 agcaatattg ccacccgaat tatcggtaat ctgccacaaa tttttctagg catggtaggg
    17281 cttgtttccg tgcttgccct tttagcacta actttttata aaagaagttc taagatgagt
    17341 gtcttttcta tcttagcacg ccttcccttt attggaatct ttgtgcagac ctacttgaca
    17401 gcctattatg cacgtgaatg ggggaatatg atttcacagg gaatggagct gacgcagatt
    17461 tttcaaatga tgcaggaaca aggttcccag ctctttaaag aaatcggtca agatctggct
    17521 caaaccctga aaaatggccg tgaattttct cagacgatag gaacctatcc tttctttagg
    17581 aaggaattga gtctcatcat agagtatggg gaagttaagt ccaagctggg tagtgagttg
    17641 gaaatctatg ctgaaaaaac ttgggaagcc ttttttaccc gagtcaaccg caccatgaat
    17701 ttggtgcagc cactggtttt tatctttgtg gcactgatta tcgttttact ttatgcggca
    17761 atgctcatgc ccatgtatca aaatatggag gtaaattttt aaaatgaaaa aaatgatgac
    17821 attcttgaaa aaagctaagg ttaaagcttt tacattggtg gagatgttag tagtcttgct
    17881 gattatcagc gtgcttttct tgctctttgt acctaatctg accaagcaaa aagaagcagt
    17941 caatgacaaa ggaaaagcag ctgttgttaa ggtggtggaa agccaggcag aactttatag
    18001 cttggataaa aatgaagatg ctactctgag caagttacaa ggagatgggc gcatcacgga
    18061 agaacaggtt aaagcttata atgaatacta tactaaaaat ggaggggcaa atcgtaaagt
    18121 caatgattaa ggcctttacc atgctggaaa gtctcttggt tttgggactt gtgagtatcc
    18181 ttgccttggg cttatccagc tctgtccagt ccacttttgc agcagtagag gagcagattt
    18241 tctttatgga gtttgaagaa ctctatcggg aaacccaaaa acgcagtgta gccagtcagc
    18301 aaaagactag tctgaacttg gatgggcaga tgattagcaa tggcagtcaa aagttgacag
    18361 ttcctaaagg aattcaggca ccatcaggcc aaagtattac atttgaccga gctgggggca
    18421 attcgtccct ggctaaggtt gaatttcaga ccagtaaagg agcgattcgc tatcaattat
    18481 atctaggaaa tggaaaaatt aaacgcatta aggaaacaaa aaattagggc agtgatttta
    18541 ctggaagcag tagtcgctct agctatcttt gccagcattg cgaccctcct tttgggacaa
    18601 attcaaaaaa ataggcaaga ggaagcaaaa atcttgcaaa aggaagaagt cttgagggta
    18661 gctaagatgg ccctgcagac ggggcaaaat caggtaagca tcaacggagt tgagattcag
    18721 gtattttcta gtgaaaaagg attggaggtc taccatggtt cagaacagtt gttggcaatc
    18781 aaagagccat aaggtcaagg cttttacctt gttagaatcc ctgcttgccc tgcttgtcat
    18841 cagtggggga ttactccttt ttcaagctat gagtcagctc ctcatttcag aagttcgcta
    18901 ccagcaacaa agcgagcaaa aggagtggct cttgtttgtg gaccaacttg aggtagaatt
    18961 agaccgttcg cagttcgaaa aagtagaagg caatcgccta tacatgaagc aagatggcaa
    19021 ggacatcgcc atcggtaagt caaagtcaga tgatttccgt aaaacgaatg ctcgtggtcg
    19081 aggttatcag cctatggttt atggactcaa atctgtacgg attacagagg acaatcaact
    19141 ggttcgcttt catttccagt tccaaaaagg cttagaaagg gagttcatcc atcgtgtgga
    19201 aaaagaaaaa agttaaggca ggtgttctcc tctacgcagt caccatagca gccatcttta
    19261 gtcttttgtt gcaattttat ttgaaccgac aagtcgccca ctatcaagac tatgctttga
    19321 ataaagaaaa attggttgct tttgctatgg ctaaacgaac caaagataag gttgagcaag
    19381 aaagtgggga acagtttttt aatctaggtc aggtaagcta tcaaaacaag aaaactggct
    19441 tagtgacgag ggttcgtacg gataagagcc aatatgagtt tctgtttcct tcagtcaaaa
    19501 tcaaagaaga gaaaagagat aaaaaggaag aggtagcgac cgattcaagc gaaaaagtgg
    19561 agaagaaaaa atcagaagag aagcctgaaa agaaagagaa ttcatagtca attcaactat
    19621 aatgcgttga atccagaata gtccactgta gtttctagaa aattgctgga aatggatgtt
    19681 aagctccaat tcatttgttt atatcttatt tcagtttact atactttgtg ctaaattaaa
    19741 gatatgaaac atgattttaa ccacaaagca gaaactttcg attcccctaa aaatatcttc
    19801 ctcgcaaact tggtatgtca agcagccgag aaacagattg atcttctatc agacaaagaa
    19861 attttagatt tcggtggtgg cacgggtcta ttagccttgc ccctaacccc tagccaagca
    19921 ggctaagtca gtcactcttg tagacatttc tgagaaaatg ttggagcaag ctcgtttgaa
    19981 agtggagcag caagcaatca agaatatcca gtttttggag caagatttac cgaaaaatcc
    20041 cttggagaaa gagtttgatt gccttgctgt tagtcgggtt cttcatcata tgcctgattt
    20101 ggatgcggct ctctcactgt ttcatcaaca tttgaaggaa gatgggaaac tcatcattgc
    20161 tgattttacc aagacagaag ctaatcatca tggatttgat ttagctgaac tggaaaacaa
    20221 gctaattgag catggttttt catctgtgca tagtcagatt ctctatagtg ctgaagacct
    20281 gtttcaagga aatcactcag aattcttttt aatagtagcc caaaaatcac tcgcctagtc
    20341 agggagtgat ttttctataa ggatggaaaa aagaagggaa atttggtaag ataggaatat
    20401 ggattttgaa aaaattgaac aagcttatac ctatttacta gagaatgtcc aagtcatcca
    20461 aagtgatttg gcgaccaact tttatgacgc cttggtggag caaaatagca tctatctgga
    20521 tggtgaaact gagctaaacc aggtcaagga gaacaatcaa acccttaagc gtttagcact
    20581 acgcaaagaa gaatggctca agacctacca gtttctcttg atgaaggctg ggcaaacaga
    20641 acccttgcag gccaatcacc agtttacacc ggatgctatt gctttgcttt tggtgtttat
    20701 tgtggaagag ttgtttaaag aggaggaaat tactatcctc gaaatgggtt ctgggatggg
    20761 aattctaggc gctattttct tgacctcgct tactaaaaag gtggattact tgggaatgga
    20821 agtggatgat ttgctgattg atctggcagc tagcatggca gatgtaattg gtttgcaggc
    20881 tggctttgtc caaggagatg ccgttcgccc acaaatgctc aaagaaagcg atgtggtcat
    20941 cagtgacttg cctgtcggct attatcctga tgatgccgtt gcgtcgcgcc atcaagttgc
    21001 ttctagccaa gaacatactt acgcccatca cttgctcatg gaacaagggc ttaagtacct
    21061 caagtcagac ggatacgcta tttttctagc tccgagtgat ttgttgacca gtcctcaaag
    21121 tgatttgtta aaagaatggc tgaaagaaga ggcgagtctg gttgctatga ttagtctgcc
    21181 tgaaaatctc tttgctaatg ccaaacaatc taagactatt tttatcttac agaagaaaaa
    21241 tgaaatagca gtagagcctt ttgtttatcc acttgctagc ttgcaagatg caagtgtttt
    21301 aatgaaattt aaagaaaatt ttcaaaaatg gactcaaggt actgaaatat aaaatagatt
    21361 ttgttataat agttgaaaac gcttaaaaag gggtatcatg ttatgacaaa aacaattgca
    21421 atcaatgcag gaagttcaag tttgaaatgg caattatact taatgccaga agaaaaagta
    21481 ttggcgaaag gtttgattga acgtatcggt ttgaaagatt caatttcaac tgtaaaattt
    21541 gacggccgtt ctgaacaaca aattttggat attgaaaatc atatacaagc cgttaaaatt
    21601 ttattggatg acttgattcg tttcgatatt atcaaggctt atgacgagat tacaggtgtt
    21661 ggacatcgtg ttgttgctgg tggagaatat ttcaaagaat caacagttgt tgagggagat
    21721 gttttagaaa aagttgaaga gttgagtttg ttggctcctc tacacaaccc ggccaatgca
    21781 gcaggtgttc gtgccttcaa ggaattgttg ccagacatta ccagtgtagt tgtttttgat
    21841 acttccttcc acacaagtat gccagagaaa gcttatcgct accctctacc aacaaaatat
    21901 tacacagaaa acaaggttcg taaatacggt gctcatggta caagtcacca gtttgtagca
    21961 ggagaagctg caaaactctt gggacgtcca ttagaagact tgaagttaat tacctgtcat
    22021 attggtaacg gaggctcaat tacagctgtg aaagccggca aatctgtaga cacttctatg
    22081 gggttcactc ctcttggtgg tattatgatg ggaacgcgta caggggatat tgatccagct
    22141 atcattcctt atttaatgca atatacagag gattttaaca caccagaaga tatcagtcgt
    22201 gttcttaacc gtgaatcagg tcttttggga gtttctgcta attctagcga tatgcgcgat
    22261 atagaagcag ctgtagcaga agggaatcac gaggctagct tggcttatga aatgtatgtt
    22321 gaccgtatcc aaaaacatat cggtcagtac cttgcagtgc taaatggagc agatgccatt
    22381 gttttcacag caggtgtcgg tgaaaatgca gagagtttcc gtcgtgatgt aatctcaggg
    22441 atttcgtggt ttggttgtga tgttgatgat gaaaagaatg tctttggcgt tacaggagac
    22501 atctcaacag aggcagctaa aatccgtgtc ttggttattc caacagatga agaattagtc
    22561 attgcccgtg acgttgaacg cttgaaaaaa taagtgaaac taaaaaaata ttcaatacaa
    22621 ggagttggga aagttatttt tccagcttct ttttctgatg aaattgtcca aaaccttgct
    22681 atgattggct tttttgaaaa atatggtata atagtagtaa tttaatagat ggagttgagt
    22741 tttgaagaaa aactttcgtg taaaaagaga gaaagatttt aaggcgattt tcaaggaggg
    22801 gacaagtttt gctaatcgca aatttgtggt ctaccaatta gaaaaccaga aaaaccattt
    22861 tcgagtaggt ctatcagtta gcaaaaaact ggggaatgcc gtcactagaa atcaaattaa
    22921 gcgacggatt cggcatatta tccagaatgc aaaagggagt ctggtagaag atgtcgactt
    22981 tgttgtcatt gctcgaaaag gagtcgaaac cttgggatac gcagagatgg agaaaaatct
    23041 actccatgta ttaaaattat caaagattta ccgggaagga aatgggagtg aaaaagaaac
    23101 taaagttgac tagtttgcta ggactgtctc tgttaatcat gacagcctgt gcgactaatg
    23161 gggtaactag cgatattaca gccgaatcgg ctgatttttg gagtaaattg gtttacttct
    23221 ttgcggaaat cattcgcttt ttatcgtttg atattagtat cggagtgggg attattctct
    23281 ttacggtctt gattcgtaca gtcctcttgc cagtctttca ggtgcaaatg gtggcttcta
    23341 ggaaaatgca ggaagctcag ccacgcatta aggcgcttcg agaacaatat ccaggtcgag
    23401 atatggaaag cagaaccaaa ctagagcagg aaatgcgtaa agtatttaaa gaaatgggtg
    23461 tcagacagtc agactctctt tggccgattt tgattcagat gccggttatt ttggccctgt
    23521 tccaagccct atcaagagtt gactttttaa agacaggtca tttcttatgg attaaccttg
    23581 gtagtgtgga tacaaccctt gttcttccga ttttagcagc agtattcacc tttttaagta
    23641 cttggttgtc caacaaagct ttgtctgaac gaaatggcgc tacgactgcg atgatgtatg
    23701 ggattccagt cttgattttt atctttgcag tttatgcgcc aggtggagtc gccctatact
    23761 ggacagtgtc taatgcttat caagtcttgc aaacctattt cttgaataat ccattcaaga
    23821 ttatcgcaga gcgcgaggcc gtagtacagg cacaaaaaga tttggaaaat agaaaaagaa
    23881 aagccaagaa aaaggctcag aaaacgaaat aaataaggag gaatctggta atggtagtat
    23941 ttacaggttc aactgttgaa gaagcaatcc agaaaggatt gaaagaatta gatattccaa
    24001 gaatgaaggc tcatatcaaa gtcatttcta gggagaaaaa aggctttctt ggtctatttg
    24061 gtaaaaaacc agcccaagtg gatattgaag cgattagtga aacgactgtt gtcaaagcaa
    24121 atcaacaggt agtaaaaggc gttccgaaaa aaatcaatga tttgaacgag cctgtgaaga
    24181 cggttagtga agaaaccgtt gaccttggtc atgtggttaa tgctattaaa aaaatagagg
    24241 aagaaggtca aggtatttct gatgaagtca aggctgaaat cttaaaacat gaaagacatg
    24301 ccagcactat cttagaagaa actggtcaca ttgagatttt aaatgaactt caaatcgagg
    24361 aagcgatgag ggaagaagca ggcgctgatg accttgaaac tgagcaagat caaactgaaa
    24421 atcaagactt gaaagagatg ggcttgaagg tcgagcaaag ttatgatatt gcccaggtgg
    24481 ctacggatgt gactgcctat gttcaagcga ttgtggatga catggatgtt gaagctacac
    24541 tttcaaatga ttataaccgt cgtagcatca atctacaaat tgacaccaac gaaccaggtc
    24601 gtattatcgg ctaccatggt aaagtcttga aggccttgca actgttggct caaaattatc
    24661 tttacaaccg ctattccaaa accttctacg ttacaatcaa tgtcaatgat tatgtcgaac
    24721 accgtgcaga agtcttgcag acctatgcgc aaaaattggc gaatcgtgtt ttggaagaag
    24781 gtcgcagtca taaaacagat ccaatgtcaa atagcgaacg caagattatc catcgtatta
    24841 tttcacgtat ggatggcgtg actagttact ctgaaggtga tgagccaaat cgctatgttg
    24901 ttgtagatac agaataagta aaatcagggt tgacctgatt ttttgctagt tagaggaggt
    24961 taaactgatg ttgaataaga taagagacta tctagacttt tctggtttgc agtaccgtag
    25021 tcctgataaa gcgggagcag agcgagagaa gatgctggcg ttccgccaca aaggacaaga
    25081 ggcccgaaag gcttttacag aactggccaa agcctttcaa gcaagccatc cagaatggca
    25141 actccaacag actagtcagt ggatgaatca ggcccagcgt ttgagaccac atttttgggt
    25201 ttatctacag agagacggac aagtgacaga acctatgatg gccttacgtt tgtatgggac
    25261 atctactgac tttggaattt ctttggaagt cagtttcatc gaacgtaaga aggatgagca
    25321 aacactgggc aagcaggcca aagttttaga cattccaacc gttaaaggga tttattatct
    25381 aacctactct aatggtcaaa gtcaacggtg ggaggcgaat gaagaaaagc gtcgtacttt
    25441 acgcgagaag gtgagaagtc aagaagttcg aaaagtttta gtgaaggtag atgttcctat
    25501 gacagaaaat tcgtctgaag aagaaatcgt agaaggctta ttgaagtctt attctaaaat
    25561 tcttccctat tatctagcta cgagaaaata agataatttg taaaacatca taaatcatac
    25621 agtccaagag tgaacagtcc gctgtgtaat tcttggtctt tttgtttgcg ctttcgcatt
    25681 atataataaa cttacaaaaa caattcaaaa ggagaacaat tatggaagtc gtttcaagtg
    25741 ttctaaattg gttttctagc aatattttgc agaatcccgc atttttcgta ggtttattgg
    25801 tgttgatagg atatgcactt ttgaaaaaac ctgcccatga cgttttttca gggtttgtta
    25861 aagcaacagt agggtatatg ttgcttaacg tgggtgctgg tggtttggtt acaacctttc
    25921 gtccaatctt agcagctctt aactacaaat tccaaattgg tgcagcggtt atcgaccctt
    25981 attttggact tgctgcagca aacaacaaaa ttgtagcaga gtttccagat tttgttggaa
    26041 ctgcaactac agctctattg attggttttg gaataaatat cttgctcgta gctcttcgaa
    26101 agattacgaa ggtaagaacc ctctttatta ctggtcacat catggtacaa caagctgcaa
    26161 cagtatctct tatggttcta ttcttagtac cacaattgcg caatgcttac ggtacagcag
    26221 cgattggtat catctgtgga ctttactggg cagttagttc aaatatgact gttgaggcaa
    26281 ctcaacgctt gactggtggt ggcggatttg cgattggtca ccaacagcaa tttgcaatct
    26341 ggtttgtaga taaagtagca ggacgctttg gtaagaaaga agaaagttta gacaatctta
    26401 aattacctaa gttcctctca atcttccacg atacagttgt tgcatctgct accttgatgc
    26461 tcgtattctt cggagccatt cttttaatct tgggtccaga cattatgtct aataaagaag
    26521 tcatcacttc aggaactcta ttcaatcctg ctaaacaaga tttctttatg tacattatcc
    26581 aaacagcctt taccttctca gtttacttgt tcgttttgat gcaaggtgtc cgaatgttcg
    26641 tatctgagtt gacaaacgcc ttccaaggta tttcaaacaa attgttgcca ggttcattcc
    26701 cagcggttga cgttgcagct tcttatggat ttggttctcc aaatgctgtc ttgtcaggat
    26761 ttacctttgg tttgattggt caattgatta caattgtttt gctcatcgtc tttaaaaatc
    26821 cgattcttat tattacagga tttgtaccag tgttctttga caatgcagcc attgcggtct
    26881 acgctgataa acgcggcgga tggaaagcgg ctgttatcct ttcctttata tcaggtgtcc
    26941 ttcaagttgc tctaggagct ctttgtgtgg cccttctcga tttggcatct tatggtggct
    27001 accatggaaa tatcgacttt gaattcccat ggcttggatt tggatatatc ttcaaatacc
    27061 ttggtattgt tggttatgta cttgtgtgtc tcttctggct tgttattcct caacttcaat
    27121 ttgccaaagc aaaagataaa gagaaatatt acaacggtga agttcaagaa gaagcttagt
    27181 atctagaaaa ggagaaataa aatggttaaa gtattagcag cgtgcggaaa tggaatgggt
    27241 tcatcaatgg ttatcaagat gaaggttgaa aatgctctcc gtaagcttaa tcaaacagat
    27301 tttacagtca attcatgcag tgtcggtgaa gctaaaggtt tagcagtagg atatgacatt
    27361 gtaatcgctt ctcttcattt gattcaagaa ttggaagggc gaactaatgg gaagttaatt
    27421 gggcttgata acttgatgga tgataaagaa atcaccgaaa aactcagtca agcactacag
    27481 taaaaggttg gagggggctg gacagaaact gagagttatc gtttctgtcc agccccctct
    27541 ttaaataaag gaggcagata tgaatttaaa acaagcttta attgacaacg actcgatccg
    27601 actaggttta gaggctaacg attggaaaga agcagtcaag gtagcagtag atcccttgat
    27661 tgaaagtggg gcaattttgc cagagtatta cgatgctatc attgaatcga ctgaagagta
    27721 tgggccttac tatatcttga tgccaggtat ggctatgccc cacgctagac ctgaagctgg
    27781 tgtgcaaagt gatgcctttt cattaattac cttacaaaat cctgttgtat tttcagatgg
    27841 gaaagaggta tctgttttgt tggcactagc agcaacaagt tcaaaaattc acacaagtgt
    27901 agccattcca caaattattg ccctgtttga attagaagat tctattgcac gtttacaggc
    27961 ttgccagact aaagaagatg tcttggctat gattgaagaa tctaaggata gcccttatct
    28021 cgaaggattg gatttggaaa gttagaaaga ggaataaaga aatgacaaaa agaataccta
    28081 atttacaagt tgcattagac cattcagact tgcaaggagc gattaaagca gctgtttctg
    28141 ttggtcagga agtagatatt atcgaagctg gaactgtttg cttgcttcaa gttggaagtg
    28201 aactggctga agtcttgcgt agccttttcc cagataagat tattgtggca gacacaaaat
    28261 gtgctgatgc tggtggaaca gttgctaaaa ataatgcggt tcgtggagca gactggatga
    28321 cttgtatctg ttgtgcaacc atccctacta tggaagcagc tctaaaggct atcaagactg
    28381 aacgaggaga acgaggcgaa atccagatcg agctttatgg cgattggact tttgaacaag
    28441 ctcagctttg gctagatgca ggtatctcac aagctattta tcaccaatct cgtgatgctc
    28501 ttcttgctgg tgaaacttgg ggtgaaaaag accttaataa ggttaaaaaa ctcattgaca
    28561 tgggcttccg tgtatctgta acaggtggtc tagatgtaga tactctcaaa ctctttgaag
    28621 gtgttgatgt ctttaccttt atcgcaggtc gtggaattac agaggctgtg gatccagcag
    28681 gagcagcgcg tgccttcaag gatgaaatca aacgaatttg ggggtaaatc atggtacgtc
    28741 caattggaat ttatgaaaag gcaaccccaa cacactttac ttggctagaa cgtttaaatt
    28801 ttgccaagga gttaggcttt gattttatcg agatgtctat tgacgaacgt gacgagcgtt
    28861 tagcaagact tgactggagt aaggaagaac gcttggaagt tgtcaaagaa atctatgaaa
    28921 ctggtgttcg tattccttct atctgttttt caggccatcg tcgctaccca ttgggatcaa
    28981 aagattcagt tctagaggaa aaatctctag aactcatgaa aaaatgtatc gaattagctc
    29041 aagacttggg agttcgtacg attcaattag ctggttacga tgtttactat gaggaaaagt
    29101 caccccagac acgccaacgt tttatcaaaa atttgagaaa atcctgtgac tgggctgaag
    29161 aagctcaggt ggtacttgct attgaaatta tggatgatcc tttcatcagt agcatcgaaa
    29221 aatatttggc tatagaaaaa gagattgact ctcccttcct ctttgtatat ccagatattg
    29281 gtaatgtgtc tgcatggcat aatgatatct atagtgagtt ttatcttggt catcatgcca
    29341 tcgcagctct ccatctcaag gatacttatg cagtgacaga aagttcaaag ggccagttcc
    29401 gagatgtacc tttcgggcaa ggttgtgtca aatgggaaga agctttcgat attttaaagg
    29461 aaaccaatta taatggacct ttcctaatcg aaatgtggtc tgaaaattgt gaaacagtag
    29521 aagaaacacg cgcagccatt caagaggcgc aagcttttct ctatccactc attaagaaag
    29581 caggtttgat gtaagatgaa tcaagtaatc aatgctatgc gtaaacgagt ctgtgatgcc
    29641 aatcaatcat tgccaaaaca tggacttgtc aaatttacct gggggaatgt atctgaagtt
    29701 aatcgcgaac tcggtgtcat tgttatcaaa ccatcaggcg tggattatga cgaattgaca
    29761 cctgaaaaca tggtagtgac tgatctagat ggtaagatcc tagaagggga tttaagacca
    29821 tcttccgacc tcccaactca tgtgcaatta tataaggctt ggtcagaaat tggtagtgtg
    29881 gttcacaccc attcgacaga agctgttggt tgggctcagg caggtcgtga tattcctttc
    29941 tacggaacaa cccatgcaga ttatttctac ggttcaatcc cttgcgcccg tagtttgacc
    30001 aaggacgaag tagaagtggc ctatgaaaaa gatactggcc tggttatcgt agaagagttt
    30061 gaacatcgcg gacttaaccc ggttgaagta ccaggaattg ttgtacgcaa tcacggtcca
    30121 ttcacctggg gcaaaaatcc agagaatgct gtttatcact ctgtcgtact agaggaagta
    30181 tcaaagatga atcgctttac agaacaaatc aatccaagag ttggacctgc tccccagtac
    30241 atactagaaa aacactacca acgtaaacat ggaccaaatg cttattatgg tcaaaagtaa
    30301 gaacgatgaa ggaggagaaa aagataaatt tagctcctct ttttacattt gatttttatt
    30361 gagagtaaag ttggaattga agtaatttta aaagattttt tagaaatagc gcttgatata
    30421 tatatggtaa aataaaaaga attgctgtga tatcaataga tttgggggat tttttaatat
    30481 ggtactggat aaggcaagtt gtgatttgct tcaatatttg atggatcaag aaacgtccaa
    30541 aacgattatg gcgatttcga aagatttgaa agagtcaaga aggaaaattt attatcacat
    30601 tgacaaaatc aatgctgctc tgggtgacga ggcgcttcac atcattagta ttccacgaat
    30661 tggtattcac ttaacggaag agcagagaga tgcttgttgt aaactattat cggaagtaga
    30721 ttcgtacgat tatatcatga gtgcgcatga acgtatgatg ataatgttac tatggatagg
    30781 tatttctaaa gaacgtatta cgattgaaaa attgatagag ttaacagagg tatctaggaa
    30841 tactgttctc aatgatttga atagtattcg ttatcaacta actttggaac aatatcaggt
    30901 gatcttgcaa gtgagcaagt cacagggata ccaccttcat gcccaccctc ttaataaaat
    30961 tcagtatctt caatcgcttc tatatcatat ttttatggaa gaaaatgcca cttttgtatc
    31021 tattttagaa gataagatga aagagaggtt agatgatgag tgtttgcttt ctgttgaaat
    31081 gaaccaattt tttaaggaac aggttccttt agttgaacaa gatttaggga agaaaataaa
    31141 ccatcatgaa ataactttta tgttgcaggt tctaccttat ttgctgttaa gctgtcataa
    31201 tgttgaacag tatcaagaaa gacatcagga tatagagaaa gaattttctt tgataagaaa
    31261 aagaatagag tatcaggtgt ctaagaaatt aggagaacgg ctgtttcaaa agtttgaaat
    31321 ttctttgtca ggacttgaag tttctcttgt agctgttctc ctcctctcct atcgtaaaga
    31381 tttggatatt catgcagaaa gtgatgattt tcggcaatta aaacttgctt tagaagaatt
    31441 tatctggtat tttgaatcac aaatccgaat ggagattgag aacaaggatg atttgttacg
    31501 aaatttgatg atccactgta aagccttgtt atttagaaag acttacggta ttttttctaa
    31561 aaatcctcta acaaaacaaa ttcgatccaa gtatggagaa ttatttttag tcactagaaa
    31621 atctgcggaa attttagaag gagcatggtt tattcggcta acagacgatg atattgccta
    31681 tttgacgatt catattggag gatttttaaa atatacacca tcgtctcaaa aaaatatgaa
    31741 aaaagtttat ctcgtttgtg atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg
    31801 caaactttat tttccaaatg agcaaattga cactgtattt acaacagaac aatttaagag
    31861 tgtggaagat attgcacaag ttgatgtagt gattactact aatgatgatt tggatagcag
    31921 atttccgatt ttaagggtta atcctatcct tgaagcagaa gatattttga aaatgctaga
    31981 ctatcttaaa cacaatatat ttcgtaataa gagcaaaagt ttcagtgaaa atctttctag
    32041 tcttatttcg tcttatattg tagacagcaa gttggctagt aagttccaag aagaggttca
    32101 aacacttata aatcaagaaa tagtagttca agcttttttg gaagatattt gaaggacagt
    32161 ccaatgatga acacaaacct gtgtttttct tggtcttttt tagtgttttg aagggtggta
    32221 tactaatctc aaagataaca attatatcca aaggaggcaa catatgccaa acgtcaaaga
    32281 aattacaaga gagtcatgga ttttagccac tttcccagag tggggaacat ggttgaacga
    32341 agaaatcgaa gaagaagtcg tacctgaagg caactttgcc atgtggtggc taggcaactg
    32401 tggtacttgg attaagacac cagctggtgc taacgttgtc atggaccttt ggtcaaaccg
    32461 tggaaaatca accaaaaaag tgaaagatat ggttcgtggg caccaaatgg caaatatggc
    32521 aggtgttcgt aagttgcaac caaacttgcg tgttcagcca atggttatcg atccatttgc
    32581 tatcaacgaa ctagactatt acttagtttc acacttccac agtgatcata tcgacccata
    32641 cacagctgca gcaattctca acaatcctaa gttagagcat gttaagttta tcggtcctta
    32701 ccactgtgga cgaatctggg aaggatgggg tgttccaaaa gaacgtatca tcgttgttaa
    32761 accaggtgac actatcgaat taaaagatat gaagattcat gcagtagaat catttgaccg
    32821 tacttgcttg gtaactctcc cagtgaacgg tgctgatgag acaggcggtg aacttgctgg
    32881 cttggctgtt acagatgaag aaatggctca aaaggctgtt aactatatct ttgaaacacc
    32941 aggtggaacc atctatcatg gtgcagattc tcacttctca aactattttg caaaacatgg
    33001 taaagacttt aaaattgatg ttgctttgaa taactatggt gaaaatccgg taggtatcca
    33061 agacaaaatg acatctatcg accttcttcg tatggcagaa aatctgcgta ccaaagtcat
    33121 tatcccagtt cactatgata tctggtctaa cttcatggct tctactaatg agattctaga
    33181 actttggaaa atgcgaaaag atcgcttgca atacgatttc catccattta tctgggaagt
    33241 tggcggtaag tacacttatc ctcaagatca acacttagta gaataccatc atccacgtgg
    33301 ttttgatgat tgttttgaac aagactctaa cattcaattt aaagctttgc tataatataa
    33361 aaatattctt tggaggttat ccgtatgttg catgatacgg ataattttca tataatagta
    33421 aaatagaatg tgtgattcaa taatcacctc aaatagaaag gaaattctat gtcaaatcta
    33481 tctgttaatg caattcgttt tctaggtatt gacgccatta ataaagccaa ctcaggtcat
    33541 ccaggtgtgg ttatgggagc ggctccgatg gcttacagcc tctttacaaa acaacttcat
    33601 atcaatccag ctcaaccaaa ctggattaac cgcgaccgct ttattctttc agcaggtcat
    33661 ggttcaatgc tcctttatgc tcttcttcac ctttctggtt ttgaagatgt cagcatggat
    33721 gagattaaga gtttccgtca atggggttca aaaacaccag gtcacccaga atttggtcat
    33781 acggcaggga ttgatgctac gacaggtcct ctagggcaag gtatttcaac tgctactggt
    33841 tttgcccaag cagaacgttt cttggcagcc aaatataacc gtgaaggcta caatatcttt
    33901 gaccactata cttacgttat ctgtggagac ggagacttga tggaaggtgt ctcaagcgag
    33961 gcagcttcat acgcaggttt gcaaaaactt gataagttgg ttgttcttta tgattcaaat
    34021 gatatcaact tggatggtga gacaaaggat tcctttacag aaagtgttcg tgaccgttac
    34081 aatgcctacg gttggcatac tgccttggtt gaaaatggaa cagacttgga agccatccat
    34141 gctgctatcg aaacagcaaa agcttcaggc aagccatctt tgattgaagt gaagacggtt
    34201 attggatacg gttctccaaa caaacaagga actaatgctg tacacggcgc ccctcttgga
    34261 gcagatgaaa ctgcatcaac tcgtcaagcc ctcggttggg actacgaacc atttgaaatt
    34321 ccagaacaag tatatgctga tttcaaagaa catgttgcag accgtggcgc atcagcttat
    34381 caagcttgga ctaaattagt tgcagattat aaagaagctc atccagaact ggctgcagaa
    34441 gtagaagcca tcatcgacgg acgtgatcca gtcgaagtga ctccagcaga cttcccagct
    34501 ttagaaaatg gtttttctca agcaactcgt aattcaagtc aagatgcctt gaatgttgtg
    34561 gcagctaaat taccaacctt tctaggtgga tcagctgacc ttgctcactc aaacatgact
    34621 tatatcaaga cggatggact tcaagacgat gccaatcgct tgaatcgtaa cattcagttt
    34681 ggtgttcgtg aatttgcaat gggaacaatc ttgaacggga tggcccttca tggtggactt
    34741 cgtgtatacg gtggaacttt cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg
    34801 tcagccttac aaggacttcc tgtgacttat gtctttaccc atgattcaat cgcagttggg
    34861 gaagatggtc cgactcacga accagttgag catttagcag gtcttcgtgc tatgccaaat
    34921 ctaaatgttt tccgtccagc agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg
    34981 acaagtgaga aaacaccaac tgcccttgtc ttgacacgtc aaaacttgac tgtcgaagag
    35041 ggaacagact tcgacaaggt tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac
    35101 tttgatacca tcttgattgc gacaggttca gaggttaatc ttgctgtctc agctgctaaa
    35161 gaattggcta gtcaaggcga aaacatccgc gtagtcagca tgccatctac agatgtcttt
    35221 gataaacaag atgcagctta caaggaagaa attcttccaa atgcagtccg ccgtcgtgtt
    35281 gcagtcgaaa tgggtgcaag tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt
    35341 ctaggtattg ataccttcgg agcctctgcc ccagcaccaa aagtattggc agaatatggc
    35401 tttactgtcg aaaatcttgt aaaagttgtt cgaaatttga aataatccta aaaatcaggg
    35461 cgtaagctct ggtttttctt accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa
    35521 tttgatatag tagtcctatg taaaagacaa aggagaaaac aatgaatcca aatattactt
    35581 ttttaatcat gcttgtaggt atgatggcct tgatgttctt tatgcaacgt tctcaaaaga
    35641 aacaagctca aaagcgtatg gaaagcttaa ataaactaca aaaaggctat gaagtgatta
    35701 caatcggtgg actttatgga acagtcgatg aagtagatac ggagaaggga acaatagttc
    35761 ttgacgtaga tggagtttac ttgacttttg aactagctgc tatcaagaca gtattaccgc
    35821 tgaaagaaac agcttcacta gaaggcgcaa ttgaaaaata agacgggatc acgaactccc
    35881 gttttctata aaagaaagga aatgggatga aaaaattagt ctttgtctgt ctgggaaata
    35941 tttgccgtag ccctatggcc gagtttgtta tgaaatcaat gacagataac tacgaaatcc
    36001 aaagtcgagc aacttcctct tgggaacatg gcaatccgat tcataagggg actcagggaa
    36061 tttttcaaga gtatgagatt ccttatgaca agaacaagac atcgcttcag attagtaagg
    36121 aagattttga agcctttgat tatattatcg gaatggacgc ttcaaatatt tctgacttac
    36181 gtcagatgtg tccagtagac tgtcaagata agatttactc attttcatct gaaagtgttc
    36241 cagacccttg gtatacagga gattttgaag aaacctatcg acgtgttcaa gagggctgtc
    36301 aagcttggtt agaacgttta gaaaaggaga gttaaggtgg aaaatcttac gaatttttac
    36361 gaaaagtatc gtgtctatct gactcgtcca cgtttagagc ttttggcagt agttaccatt
    36421 gttttctgtg ctgtactcgt cttttttcta aatattccag gaaaaggtgt cttaaaactc
    36481 gataatggaa cgattgttta tgatggcagt cttgtccgtg gtaaaatgaa tggccaaggt
    36541 accattacct tccaaaatgg agaccaatat acaggtggct tcaacaatgg agccttcaac
    36601 ggaaaaggta cctttcaatc taaagaaggc tggacctacg aaggtgattt tgtaaatggt
    36661 caggctgaag gaaaagggaa actaacaaca gaacaagaag tcgtttatga aggaactttt
    36721 aaacaaggcg tttttcaaca aaaataaagc ctccttatca aaggaggtat tattagaatc
    36781 acaaggtaag cgcttacctg taaatccctt tctttccaaa tccctcttcc aagcaagttt
    36841 gtgaaataaa aaatatttga aataaatttc acaaacttca aagataaaac ttgataagaa
    36901 aagaaaatga gaaaagtttc acaagagttt aaaaattctt tgtgaaatgt ttatgaaact
    36961 gtttacaaag ttgaaataat gcgttataat aaacttgtga aaaaattaac aaaggatatc
    37021 gttccttgaa agctatggag gaaaatatgg ctgataaaaa aactgtgaca ccagaggaaa
    37081 agaaactcgt tgctgaaaag cacgtagatg agttggttca aaaagctcta gttgcccttg
    37141 aagaaatgcg taaattgaat caagagcaag ttgactacat cgttgccaaa gcatcagtag
    37201 cagctttgga tgcccatgga gaattggctt tacatgcctt tgaagaaaca ggacgtggtg
    37261 tatttgaaga taaagcaact aagaacttgt ttgcttgtga acacgtagta aacaacatgc
    37321 gccacactaa gacagttggt gttatcgaag aagacgatgt aacaggattg actcttattg
    37381 ctgaaccagt tggtgttgtt tgtggtatta ctccaacaac aaacccaaca tcaacagcaa
    37441 tcttcaaatc attgatttca ttgaagacac gtaacccaat cgtctttgcc ttccatccat
    37501 cagcacaaga atcatctgct catgcagctc gtatcgtccg cgatgcagct atcgcagctg
    37561 gtgctcctga aaactgtgtg caatggatta ctcaaccatc tatggaagca acaagtgccc
    37621 ttatgaacca cgaaggtgtt gcgacaatcc ttgcaacagg tggtaatgcc atggttaagg
    37681 cggcttattc atgtggtaaa ccagctcttg gggtaggtgc cggaaacgtt ccagcttatg
    37741 ttgaaaaatc agcaaacatt cgtcaagcag cacacgatat cgtcatgtct aaatcatttg
    37801 ataacggtat ggtctgtgca tctgaacaag cagttatcat tgataaagaa atttacgatg
    37861 aatttgtagc agagttcaaa tcttaccaca cttactttgt aaacaaaaaa gaaaaagctc
    37921 ttcttgaaga gttctgcttc ggcgtcaaag caaacagcaa aaactgtgct ggtgcaaaat
    37981 tgaacgctga catcgttggt aaaccagcaa cttggattgc agaacaagca ggatttacag
    38041 ttccagaagg aacaaacatt cttgctgcag aatgtaaaga agttggcgaa aatgagccat
    38101 tgactcgtga aaaattgtca ccagttattg cagttttgaa atctgaaagc cgtgaagatg
    38161 gtattactaa ggctcgtcaa atggttgaat ttaacggtct tggacactca gcagctatcc
    38221 acacagctga cgaagaattg actaaagaat ttggtaaagc tgttaaagct attcgtgtta
    38281 tctgtaactc accttctact tttggtggta tcggggacgt ttacaatgcc ttcttgccat
    38341 cattgacact tggatgtggt tcttacggac gcaactcagt tggggataac gttagtgcca
    38401 ttaacctctt gaatatcaaa aaagtcggaa gacggagaaa taacatgcaa tggatgaaac
    38461 ttccttcaaa aacatacttt gaacgtgatt caattcaata ccttcaaaaa tgtcgtgacg
    38521 ttgaacgtgt catgatcgtt actgaccatg ccatggtaga gcttggtttc cttgatcgta
    38581 tcatcgaaca actggacctt cgtcgcaata aggttgttta ccaaatcttt gcggatgtag
    38641 aaccggatcc agatatcaca actgtaaacc gtggtactga gattatgcgt gccttcaaac
    38701 cagataccat catcgcactc ggtggtgggt ctccaatgga tgctgccaaa gtaatgtggc
    38761 tcttctacga gcaaccagaa gtggacttcc gtgaccttgt ccaaaaattc atggatatcc
    38821 gtaaacgtgc cttcaagttc ccattgcttg gtaagaagac taaattcatc gcgattccaa
    38881 ctacatctgg tacaggatct gaagtaacac catttgccgt tatctctgat aaagcaaaca
    38941 accgtaaata cccaatcgct gactactcat tgacaccaac tgtggcaatc gtagatcctg
    39001 ctttggtatt gacagttcca ggatttgttg ctgctgatac tggtatggac gtattgactc
    39061 acgcgacaga agcatacgta tcacaaatgg ctagtgacta cactgatggt ttagcacttc
    39121 aagccattaa attggtcttt gaaaatctcg aaagctcagt taagaatgca gacttccact
    39181 cacgtgagaa aatgcataac gcttcaacaa tcgctggtat ggcctttgcc aatgccttcc
    39241 taggtatttc tcactcaatg gcccataaga ttggtgcgca attccacaca atacacggtc
    39301 gtacaaatgc tatcttgctt ccatacgtta tccgttacaa cggtacacgt ccagctaaga
    39361 cagcaacatg gcctaagtac aactactacc gtgcagatga aaaataccaa gatatcgcac
    39421 gcatgcttgg acttccagct tctactccag aagaaggggt tgaatcttac gcaaaagctg
    39481 tctacgaact cggtgaacgt attgggatcc aaatgaattt tagagaccaa ggaattgacg
    39541 aaaaagaatg gaaagaacat tctcgtgaat tagccttcct ggcttatgaa gaccaatgtt
    39601 caccagctaa cccacgtctt ccaatggtag accatatgca agaaatcatc gaagatgcat
    39661 actatggcta caaagaaaga ccaggacgcc gtaaataatt gtttattagt ctagaagcaa
    39721 gacaaaaact caatttgagg gaaagatcca gtaatttttc tatgataaaa ggcatcctat
    39781 caaggttttt gaacacctga taggatgcct ttttatgata ttgaggcctt tttgcccttt
    39841 ttgaaaaact agaatagaaa caaaatatat aatagattga aactagaata gtacacatct
    39901 gcttctaaaa cattgttaga attcgatttg actgtcctga tcgat
//
LOCUS       DAVMHG010000003        37463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMHG010000003 DAVMHG010000000
VERSION     DAVMHG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.003"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(105..1772)
                     /locus_tag="VYR08_000113"
     CDS             complement(105..1772)
                     /locus_tag="VYR08_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV3465704.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1775..2140)
                     /locus_tag="VYR08_000114"
     CDS             complement(1775..2140)
                     /locus_tag="VYR08_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3465705.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2297..2485)
                     /locus_tag="VYR08_000115"
     CDS             complement(2297..2485)
                     /locus_tag="VYR08_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV3465706.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2601..2777)
                     /locus_tag="VYR08_000116"
     CDS             complement(2601..2777)
                     /locus_tag="VYR08_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV3465707.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(2888..3085)
                     /locus_tag="VYR08_000117"
     CDS             complement(2888..3085)
                     /locus_tag="VYR08_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV3465708.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(3340..4884)
                     /locus_tag="VYR08_000118"
     CDS             complement(3340..4884)
                     /locus_tag="VYR08_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV3465709.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5097..5399
                     /gene="gatC"
                     /locus_tag="VYR08_000119"
     CDS             5097..5399
                     /gene="gatC"
                     /locus_tag="VYR08_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV3465710.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTNRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            5399..6865
                     /gene="gatA"
                     /locus_tag="VYR08_000120"
     CDS             5399..6865
                     /gene="gatA"
                     /locus_tag="VYR08_000120"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV3465711.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPATFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            6865..8307
                     /gene="gatB"
                     /locus_tag="VYR08_000121"
     CDS             6865..8307
                     /gene="gatB"
                     /locus_tag="VYR08_000121"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV3465712.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKETKGQANPQVALKLLAQELAKLKEN"
     gene            8436..8996
                     /gene="efp"
                     /locus_tag="VYR08_000122"
     CDS             8436..8996
                     /gene="efp"
                     /locus_tag="VYR08_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV3465713.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            9018..9407
                     /locus_tag="VYR08_000123"
     CDS             9018..9407
                     /locus_tag="VYR08_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3465714.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9400..9822
                     /gene="nusB"
                     /locus_tag="VYR08_000124"
     CDS             9400..9822
                     /gene="nusB"
                     /locus_tag="VYR08_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV3465715.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAHTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(9871..10098)
                     /locus_tag="VYR08_000125"
     CDS             complement(9871..10098)
                     /locus_tag="VYR08_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3465716.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(10589..10774)
                     /gene="briC"
                     /locus_tag="VYR08_000126"
     CDS             complement(10589..10774)
                     /gene="briC"
                     /locus_tag="VYR08_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV3465717.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(10933..11700)
                     /locus_tag="VYR08_000127"
     CDS             complement(10933..11700)
                     /locus_tag="VYR08_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV3465718.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(11697..12563)
                     /locus_tag="VYR08_000128"
     CDS             complement(11697..12563)
                     /locus_tag="VYR08_000128"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV3465719.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(12600..13967)
                     /gene="accC"
                     /locus_tag="VYR08_000129"
     CDS             complement(12600..13967)
                     /gene="accC"
                     /locus_tag="VYR08_000129"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV3465720.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(13979..14401)
                     /gene="fabZ"
                     /locus_tag="VYR08_000130"
     CDS             complement(13979..14401)
                     /gene="fabZ"
                     /locus_tag="VYR08_000130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV3465721.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(14398..14883)
                     /locus_tag="VYR08_000131"
     CDS             complement(14398..14883)
                     /locus_tag="VYR08_000131"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV3465722.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(14886..16121)
                     /gene="fabF"
                     /locus_tag="VYR08_000132"
     CDS             complement(14886..16121)
                     /gene="fabF"
                     /locus_tag="VYR08_000132"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV3465723.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(16143..16874)
                     /gene="fabG"
                     /locus_tag="VYR08_000133"
     CDS             complement(16143..16874)
                     /gene="fabG"
                     /locus_tag="VYR08_000133"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV3465724.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(16908..17828)
                     /gene="fabD"
                     /locus_tag="VYR08_000134"
     CDS             complement(16908..17828)
                     /gene="fabD"
                     /locus_tag="VYR08_000134"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV3465725.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(17821..18795)
                     /gene="fabK"
                     /locus_tag="VYR08_000135"
     CDS             complement(17821..18795)
                     /gene="fabK"
                     /locus_tag="VYR08_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV3465726.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(18914..19138)
                     /locus_tag="VYR08_000136"
     CDS             complement(18914..19138)
                     /locus_tag="VYR08_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3465727.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQEK"
     gene            complement(19198..20172)
                     /locus_tag="VYR08_000137"
     CDS             complement(19198..20172)
                     /locus_tag="VYR08_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV3465728.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(20172..20606)
                     /locus_tag="VYR08_000138"
     CDS             complement(20172..20606)
                     /locus_tag="VYR08_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3465729.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(20700..21485)
                     /locus_tag="VYR08_000139"
     CDS             complement(20700..21485)
                     /locus_tag="VYR08_000139"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV3465730.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            21810..23174
                     /locus_tag="VYR08_000140"
     CDS             21810..23174
                     /locus_tag="VYR08_000140"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV3465731.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            23177..23548
                     /locus_tag="VYR08_000141"
     CDS             23177..23548
                     /locus_tag="VYR08_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV3465732.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIARDKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            23762..25036
                     /gene="serS"
                     /locus_tag="VYR08_000142"
     CDS             23762..25036
                     /gene="serS"
                     /locus_tag="VYR08_000142"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV3465733.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLANYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYIGGAEVIKP"
     gene            complement(25061..25960)
                     /locus_tag="VYR08_000143"
     CDS             complement(25061..25960)
                     /locus_tag="VYR08_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV3465734.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWGLIVMIL
                     ASQSFFFFVLTRMPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(26070..26618)
                     /locus_tag="VYR08_000144"
     CDS             complement(26070..26618)
                     /locus_tag="VYR08_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV3465735.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(26836..28158)
                     /locus_tag="VYR08_000145"
     CDS             complement(26836..28158)
                     /locus_tag="VYR08_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV3465736.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(28249..28337)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(28501..30837)
                     /locus_tag="VYR08_000146"
     CDS             complement(28501..30837)
                     /locus_tag="VYR08_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV3465737.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(30941..31489)
                     /locus_tag="VYR08_000147"
     CDS             complement(30941..31489)
                     /locus_tag="VYR08_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV3465738.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(31486..31788)
                     /gene="zapA"
                     /locus_tag="VYR08_000148"
     CDS             complement(31486..31788)
                     /gene="zapA"
                     /locus_tag="VYR08_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV3465739.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSTDDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            31875..32756
                     /locus_tag="VYR08_000149"
     CDS             31875..32756
                     /locus_tag="VYR08_000149"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV3465740.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            32760..33374
                     /gene="lepB"
                     /locus_tag="VYR08_000150"
     CDS             32760..33374
                     /gene="lepB"
                     /locus_tag="VYR08_000150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV3465741.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            33653..36019
                     /locus_tag="VYR08_000151"
     CDS             33653..36019
                     /locus_tag="VYR08_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV3465742.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEVPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(36067..37350)
                     /locus_tag="VYR08_000152"
     CDS             complement(36067..37350)
                     /locus_tag="VYR08_000152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV3465743.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        10860 a         8618 c         7267 g        10718 t
ORIGIN      
        1 ttttaacgtt tatttatgaa tgttattttt gtttttatta aaaagatacc gatttcattt
       61 actaaaaaag aaaaaagaac tgatttctca gtccttcatt aatcttattc cacactaaat
      121 aggtatgggt aaacaggttg ttgaccttgg tgaatctcga cttcaacgtc ttcgaattct
      181 tctacgattt cttgagcgat ttcattggca agttcttcgc ttccgtcttc accgacatag
      241 aaggttacga tttcactgtc ttcatccaac atatgtttca aggtttcagt caatgtttgg
      301 tgcatatcag ggtttgacac aagaattttt ccatccacca tacctagatt atcgttttca
      361 tggatttcta agccatcgat cgttgtatca cgcacggctg ttgtgacgct tccgctaacg
      421 acatctctaa gagcagctgt catacgctct tgattttctt caatggactt gcttggatca
      481 aaggcaagaa gacttgtcat accttgagga agagtgcgag cctctaccac tactgctggt
      541 tgctccaaaa cttctgccgc agattgagct gccatgaaga tgttcttgtt gtttggcaag
      601 aagatgatgt tacgggcatt gacctgttca acagccttga taaagtcttc tgttgaaggg
      661 ttcatggttt gaccgccttc gataacataa tccacgcctt gagaacagaa gatatctgct
      721 agacctttac cagccaccac agcaatcaaa gcatactctt tttcttcagc cgacttgata
      781 acttgagtag cttctttctc aacctgtgct tcgtgttggt tacgcatatt gtcaactttt
      841 accttgacca agctaccata tttgagacct tcttgcataa caagtcctgg atcttctgta
      901 tgaacatgga ctttgacaat ttcatcatcg ttaacaacga ggagagaatc cccaagctca
      961 tccaagtagt tacggaattc atcgtagtca aaatctttag cataggttgg accttgctta
     1021 agagctacca tgatttcagt acagtaacca aacgtgatgt cctcagtcgc tacgtgacca
     1081 gctacagact tatgatgctc tacattgatc atctcactca tgttggcagg agtcgctaca
     1141 aagtcctcag atgcaatata ttcgccagta agggctgaaa ggaaaccttc gtagatgaag
     1201 accaatcctt gaccacctga gtccacaacg ccaacttctt tcaatactgg aagcatgtct
     1261 ggtgttttag ctagagctgt tttagcacct tccaaggctg cgcgcatgac ttcaacagtg
     1321 tcatctgttt gctcagcttt tttcttagca ccgatagcag ctccacgaga aactgtcaaa
     1381 atcgttcctt caacaggttt catcactgcc ttataggcaa cttccacacc tgattggaag
     1441 gccagagcca agtcttgacc tgttaactcg tctttatcct tgatagcttg ggaaaatcca
     1501 cggaaaagct gagacgtaat cactcctgag ttcccacgcg cacccatcaa aagccctttg
     1561 gcaagaatgc tcgctacttc tccaactgta gaagctggct tgtctgcaac ttctttagca
     1621 ccattttcaa tggtcattcc catatttgtc ccagtatctc catctggaac tggaaagacg
     1681 tttaatgaat tgacatattc agcttgctta ttcaagcgag ttgatgcagc ctgcaccatt
     1741 tcttgaaata agctagtagt aatttttgac acggttattc tcctacaact ttgatatttt
     1801 gaatgtagac atttacagtc tgagcagtaa ttccaagctg gttttccaag ctaaaggcaa
     1861 cacgctcttg aatgtttttt gacacttcac taatctttgt tccgtagctt aacacggtat
     1921 atacatcaac tgcaatactg ccatcttcgg ctgcctttac gacgacacct ttagaataat
     1981 tttccttacc tagcagggct tggaaattat ctttgagggc atttttacta gccataccga
     2041 ccacaccaaa aatctcagtt gctgctccac ctacgacggt tgcaatcact tcatctgtta
     2101 gttcgatttg accatctttt gtattaattt ttacagtcat cctttttacc tcaactagtt
     2161 gatactctat tttatcatat ttcagcccaa gtgtaaaagc agaatgatgt atcagcggat
     2221 atttactcta tttttcaaac gatgttatac ccacaataaa agaaaaaaga ccctaaggtc
     2281 tcctttgctt ttattattaa acgcgttcaa ctttacctga tttcaaagca cgagctgaag
     2341 cccaaacttt tttaggttta ccatcgataa gaacagtaac tttttgaagg tttggtttta
     2401 cggcacgttt tgtttggttc atcgcgtgtg aacggttgtt tcctgataca gtcttacgac
     2461 ctgtaaagta acatacttta gccattgtgt tttcctccta ttagatctaa tatagcggat
     2521 gtgctagcac cacataccgt actatgttat cacactttct tgttttttgc aagggaattg
     2581 gaagattttt tatttgtgtc ttaaatcagg tcttgcgtga cattttctgc tctccacatg
     2641 ccatcgttga ttaacagaac accagaatta aaattatgtg tataaaaatc atctctaact
     2701 gcagctaagg gtatagccgt caagtccaaa tcccacagct catctatcga ttttcttaca
     2761 acaatatctg aatccaaata cagtacacga gactcgctta catactttgg aataaaatac
     2821 ctaaaaaagc cgcatatgaa agtccctcaa aggggagacg ataacctttc agaatattac
     2881 tgtcaatcta aacattcaca atctcactat tcaaagtctc tagtcttttt tccatcaatt
     2941 ggaaccattc tcgcggaagg tcatcattaa aaacataaaa cttaagatta taatgatgaa
     3001 cacaaagaga ttttattgtt gtttcaactt tatccatata agcattatct gcacctaaga
     3061 caatcgcttt tttctcttct ttcacttttt atctcatttc tttttattcc catcatatgt
     3121 ttctacgtaa ccattatttt cgcctattcg ttcgtaaaac cataccagtg gagattttag
     3181 atgaagtccc attacggttt acaattttta cattacgaca cggagtttta caaatcgatt
     3241 tcatttgcca aacgtagtta gtgaggcagt tagctagttc gccaaatagc gactagcgtc
     3301 caacaatttg gaactttagt tccaattgtt ggtactgagt cacatcttct cctctaactc
     3361 tacgtctgga tacttgtcag caaaccagcg gagggcaaag tcattttcaa agagaaagac
     3421 tggttggtca aaacggtctt tggctaagat attgcgactt gacgacatcc gttcatccaa
     3481 gtcctcaggc ttgatccaac gaacggtctt tttacccatt gggttcataa ctacttctgc
     3541 attgtactcg ccttccatgc ggtgtttaaa gacttcaaac tggagttgac ctacagcgcc
     3601 tagcatgtac tcacctgttt ggtaattctt ataaagctga acggctcctt cttgcaccaa
     3661 ttgctcaatc cccttgtgga aggatttttg cttcataaca ttcttagcag aaactttcat
     3721 gaaaatttca ggagtaaagg ttggcagtgg ttcaaattca aacttgtttt taccaactgt
     3781 caaggtatcc ccaacttgat aagtaccggt atcgtaaacc ccgataatat cacctgccac
     3841 ggcattgatc acattctcac gactttccgc cataaactga gtaacattag acagtttagc
     3901 ccccttacca gtacgaggga gattgacact catgccgcgc tcaaattcgc cagatacgat
     3961 acggacaaag gcaatacggt cacggtgacg agggtccatg ttggcttgga ttttaaagac
     4021 aaagcctgag aaatccttgt cataaggatc cacaatttca ccgtctgttt tcttgtgacc
     4081 atgtggttct ggagcaaact tgaggaaggt ttcaaggaag gtctgcacac caaagtttgt
     4141 cagggctgaa ccgaaaaaga caggcgtcaa ttctccagcc agaatagctt cctctgaaaa
     4201 ctcattcccg gcttcattta aaagctcaat atcatccttg acttgctcgt agaaaggatt
     4261 gctaccaaaa agtttgtccc catcttctag actggcaaaa cgctcatccc ctttataaag
     4321 ctctaaacgt tggttataga ggtcatacaa gccctcaaag gctttcccca tcccgatagg
     4381 ccagttcata gggtagctag caatgcccaa gatttcttcc aattcttgca agagatccaa
     4441 aggctcacga ccgtcacggt ccagcttgtt cataaaggta aagactggaa tgccacgatg
     4501 tttcacaacc tcaaacaatt tcttggtttg agcctcgatc cccttggcag agtccacgac
     4561 catgaccgca gcatccaccg ccatcaaggt acgataggta tcttctgaga agtcctcgtg
     4621 ccctggcgtg tctaagatat tcacgcgctt gccgtcgtag tcaaattgca taacagatga
     4681 agtaacagaa atcccacgtt gcttctcgat atccatccaa tcagatttag caaaagtccc
     4741 tgttttcttc ccttttaccg taccagcctc acgaatttca cccccaaagt agagtaactg
     4801 ctcagtgatg gttgttttcc ccgcgtccgg gtgggagata atggcaaagg tacgacgttt
     4861 cttaatttct tcttgaatat tcataagttc tctttctttg attctctatt tttcttgttt
     4921 caatagctga gaatgatttt tacattggat tttaccattc ctttcaacac tccattatat
     4981 cggattttag catttttttc aatttctatt tcttttcact tccccctccc ttatttatag
     5041 gaaaatatgg taaaatagaa cagactaaaa atcatcattt cacgaaagga tgcaagatga
     5101 aaattacgca agaagaggta acacacgttg ccaatctttc aaaattaaga ttctctgaaa
     5161 aagaaactgc tgcctttgcg accaccttgt ctaagattgt tgacatggtt gaattgctgg
     5221 gcgaagttga cacaactggt gtcgcaccta ctacgactat ggctgaccgc aagactgtac
     5281 tccgccctga tgtggccgaa gaaggaacaa accgtgatcg cttgtttaaa aacgtacctg
     5341 aacaagacaa ctactatatc aaggtgccag ctatcctaga cgatggagga gatgcctaat
     5401 gacttttaac aataaaacta ttgaagagtt gcacaatctc cttgtctcta aggaaatttc
     5461 tgcaacagaa ttgacccaag caacacttga aaatatcaag tctcgtgagg aagccctcaa
     5521 ctcatttgtc accatcgctg aggagcaagc tcttgttcaa gctaaagcca ttgatgaagc
     5581 tggaattgat gctgacaatg tcctttcagg aattccactt gctgttaagg ataacatctc
     5641 tacagacggt attctcacaa ctgctgcctc aaaaatgctc tacaactatg agccaatctt
     5701 tgatgcgaca gctgttgcca atgcaaaaac caagggcatg attgtcgttg gaaagaccaa
     5761 catggacgaa tttgctatgg ggggttcagg tgaaacttca cactacggag caactaaaaa
     5821 cgcttgggac cacagcaagg ttcctggtgg gtcatcaagt ggttctgccg cagctgtagc
     5881 ctcaggacaa gttcgcttgt cacttggttc tgatactggt ggttccatcc gccaacctgc
     5941 taccttcaac ggaatcgttg gtctcaaacc aacctacgga acagtttcac gtttcggtct
     6001 cattgccttt ggtagctcat tagaccagat tggacctttt gctcctactg ttaaggaaaa
     6061 tgccctcttg ctcaacgcta ttgccagcga agatgctaaa gactctactt ctgctcctgt
     6121 ccgcatcgcc gactttactt caaaaatcgg ccaagacatc aagggtatga aaatcgcttt
     6181 gcctaaggaa tacctcggtg aaggaattaa cccagaggtt aaggaaacca ttctaaatgc
     6241 cgctaaacac tttgaaaaat tgggtgctat tgtcgaagaa gtcagccttc ctcactctaa
     6301 atacggagtt gccgtatact acatcatcgc ttcatcagaa gcttcatcaa acttgcaacg
     6361 cttcgacggt atccgttacg gctatcgcgc agaagatgca accaaccttg atgaaatcta
     6421 tgtaaacagc cgaagccaag gttttggtga agaggtaaaa cgtcgtatca tgctgggtac
     6481 tttcagtctt tcatcaggtt actatgatgc ctactataaa aaggctggtc aagtccgtac
     6541 cctcatcatt caagatttcg aaaaagtctt cgcggattac gatttgattt tgggtccaac
     6601 tgctccaagt gttgcctatg acttggattc tctcaaccat gacccagttg ccatgtactt
     6661 agccgaccta ttgaccatac ctgtaaactt ggcaggactg cctggaattt cgattcctgc
     6721 tggattctct caaggtctac ctgtcggact ccaattgatt ggtcccaagt actctgagga
     6781 aaccatttac caagctgctg ctgcttttga agcaacaaca gactaccaca aacaacaacc
     6841 cgtgattttt ggaggtgaca actaatgaac tttgaaacag tcatcggact tgaagtccac
     6901 gtagagctca acaccaattc aaaaatcttc tcacctactt ctgcccactt cggaaatgat
     6961 caaaatgcca acactaacgt gattgactgg tctttcccag gagttctgcc agttctcaat
     7021 aaaggggttg tcgatgccgg aatcaaggct gctcttgccc tcaacatgga catccacaaa
     7081 aagatgcact ttgaccgcaa gaactacttc tatcctgata accccaaagc ctaccaaatt
     7141 tctcaatttg atgaaccaat cggatataat ggctggattg aagtcgaact agaagacggt
     7201 acgaccaaga aaatcggtat cgaacgtgcc cacctagagg aagacgctgg taaaaacacc
     7261 catggtacag atggctactc ttatgttgac ctcaaccgcc aaggggttcc cttgattgag
     7321 attgtatctg aggcagatat gcgttctcct gaagaagcct atgcttatct gacagccctc
     7381 aaggaagtta tccagtacgc tggcatttct gacgttaaga tggaggaagg ttcgatgcgt
     7441 gtggatgcca acatctccct tcgtccttat ggtcaagaga aattcggtac caagactgaa
     7501 ttgaagaacc tcaactcctt ctcaaacgtt cgtaaaggtc ttgaatacga agtccaacgc
     7561 caggctgaaa ttcttcgctc aggtggtcaa atccgccaag aaacacgccg ttacgatgaa
     7621 gcgaataaag caaccatcct catgcgtgtc aaggaagggg ctgctgacta ccgctacttc
     7681 ccagaaccag acctacccct ctttgaaatt tctgacgagt ggattgagga aatgcggact
     7741 gagttgccag agtttccaaa agaacgtcgt gcgcgttatg tatctgacct tggtttatca
     7801 gactacgatg ctagtcagtt gactgctaat aaagtcactt ctgacttctt tgaaaaagct
     7861 gttgccctag gtggtgatgc caaacaagtc tctaactggc tccaagggga agtcgctcag
     7921 ttcttgaatg ctgaaggtaa aacactggaa caaatcgaat tgacaccaga aaacttggtt
     7981 gaaatgattg ccatcatcga agacggtact atttcatcta agattgccaa gaaagtcttt
     8041 gtccatctag ctaaaaatgg cggtggcgcg cgtgaatacg tggaaaaagc aggtatggtt
     8101 caaatttcag atccagctat cttgatccca atcatccacc aagtctttgc cgataacgaa
     8161 gctgctgttg ccgacttcaa gtcaggcaaa cgtaacgccg acaaagcctt tacaggattc
     8221 cttatgaagg aaaccaaagg ccaagccaac ccacaagttg cccttaaact acttgcacag
     8281 gaattggcga agttgaaaga aaactagaca gaacaaaacc agccctaagg ttggtttttt
     8341 cttctctacc aactcccaat aactattttg gctttatttc cagagtattt tatggtaaaa
     8401 tgaagagtaa taatatttat taaagaggta aaaacatgat tgaagcaagt aaattaaaag
     8461 ctggtatgac ctttgaaaca gctgacggca aattgattcg cgttttggaa gctagtcacc
     8521 acaaaccagg taaaggaaac acgatcatgc gtatgaaatt acgtgatgtc cgtactggtt
     8581 ctacatttga cacaagctac cgtccagagg aaaaatttga acaagctatt atcgagactg
     8641 ttccagctca atacttgtac aaaatggatg acacagcata cttcatgaat acagaaactt
     8701 acgaccaata cgaaatccct gtagtcaatg ttgaaaacga attgctttac atccttgaaa
     8761 actctgatgt gaaaatccaa ttctacggaa cggaagtgat cggtgtcacc gttcctacta
     8821 ctgttgagtt gacagttgct gaaactcaac catctatcaa aggtgctact gttacaggtt
     8881 ctggtaaacc agcaacgatg gaaactggac ttgtcgtaaa cgttccagac ttcatcgaag
     8941 caggacaaaa actcgttatc aacactgcag aaggaactta cgtttctcgt gcctaatctc
     9001 tagaaagagg tcattctatg ggaattgaag aacaacttgg cgaaatcgtt atcgccccac
     9061 gtgtacttga aaaaatcatt gctatcgcta ctgcaaaggt agagggggtt cactcttttt
     9121 caaacagatc agtgtctgat accctttcaa aactttcact cggccgtggc atttatctta
     9181 aaaacgtgga cgaagaactc acagcagata tctatctcta ccttgagtac ggagtaaaag
     9241 ttcctaaggt agcggttgct atccagaaag ctgtcaaaga tgccgtccgt aatatggctg
     9301 atgtagaact cgctgctatc aatattcacg ttgcaggtat cgtcccagat aaaacaccaa
     9361 aaccagaatt gaaagatcta tttgacgagg acttcctcaa tgactagtcc actattagaa
     9421 tctagacgcc aactccgtaa atgcgctttt caagctctca tgagccttga gttcggtacg
     9481 gatgtcgaaa ctgcttgtcg tttcgcccat actcatgatc gtgaagatac ggatgtacaa
     9541 cttccagcct ttttgataga cctcgtttct ggtgttcaag ctaaaaagga agaactagat
     9601 aagcaaatca ctcagcattt aaaagcaggt tggaccattg aacgcttaac gctcgtggag
     9661 agaaacctcc ttcgcttggg agtctttgaa atcacttcat ttgacactcc tcagctggtt
     9721 gctgttaatg aagctatcga gcttgcaaag gacttctccg atcaaaaatc tgcccgtttt
     9781 atcaatggac tgctcagcca gtttgtaaca gaagaacaat aaaaagaaaa agtgtttgat
     9841 atctatcaaa cactttttct ttgcctccca ctatctaaaa aaatgataat agatataatt
     9901 gtaaacaaaa atccagatag gttttgcatg attgagaaag ttaaaaaaac tatggcagag
     9961 aatcgttaat ctcagattgt cggtagaacg ataaacaagg gcaaaaaaga aaccaatcag
    10021 actataatat aataaactaa ttggatctct gtgagatagt atcaaatgac taagcccaaa
    10081 gataaggcct gataagatga catctaaata atacttggac tagggaaaga aggtattcat
    10141 aaaataccct ctatcaagag tctcctcaaa aacaggaccg atgattacag gcaggacaaa
    10201 agataagata gtcgataaaa aggttggttg tccatttgaa agaaccacgg taaaatactc
    10261 atcatgaata ttcctatgat taatcaaatg agcatagcgt gcccaaaaat taccgagaat
    10321 ctgacaaacc acataagttg caaataagga gaagacaaat gaccagttcc agctcttttt
    10381 cttttttaac ctccaaatta atagaaaagg aaacttccca ctaatcccac tgttaaaata
    10441 agagaataga catcagctcc taaccctaaa atgatcgtca catacaatcc aattgtttgt
    10501 ggtaaatagg tagatagtaa aataataagc aaaaatattc caaattgtct tagtttcttt
    10561 gtgtttctca tcgtactttt ttgaaagatt accctgttcg gaagccgtac ttccaagcat
    10621 ctatataaga attaagtgcc ccttgcctca tatagggagc aaattctcta taatataacc
    10681 atctactata tccatcttcc caaacagcaa gaccacctga agtttgctcc aagtcctcag
    10741 ttgaaataac tgtaaatgtt tctgtacctg tcattgcaag taccttctta aaatagattg
    10801 ttgtaggctc acatttatag tatatttctt tttttgtcta ttttatagtc tatctcctca
    10861 actggcaatt tttcgacctg aattgcattt ttccataaaa aatgagacct ttctagtctc
    10921 atttagtcat tcttagtatt ttctaaatcg ttgatagcgt tcttccagca actcttctag
    10981 cggtttttgt gaaagtctag ccagctccgt ttggagttct tttttgacac tcttaatcag
    11041 ttctttacta gaaagtccta tttcagaaat caccttatcc accacgtcca tttctaacag
    11101 ttcatgcgaa gtgattttca tcagttctgc tgcttccatg gcgcgagtac cgtccttcca
    11161 taaaatggaa gcaaagcctt ctggactgag aatggcatag atagaatttt ccagcatcca
    11221 gacacggtcc gcgacagcta gagccagagc cccgcctgaa ccaccttcac cgataataat
    11281 ggcgataata ggaactttca ggtcactcat ttccatgaga ttgcgagcga tagcttcccc
    11341 ttgaccacgt tcttccgctc cgacaccagg ataagcacct gctgtattga taaaggtcac
    11401 aactggacgg ccaaatttct cagcctgttt catcaaccgc agtgcctttc ggtagccttc
    11461 tggatgtggt tggccaaaat tccgtttgag gttgtcttgc aaactcttgc ctttttggat
    11521 accaaccact gttacagctt gatctccaag ccaaccaata ccaccaacaa ctgcaccatc
    11581 atcacgaaaa gaacggtcac catgtaattg gataaattca tcaaaaatgc ctgtcgcaaa
    11641 atccaaggtt gtcaagcgac tctgctcacg cgcttctctg actatttttg caatattcat
    11701 ctaggactcc ctccatgcaa tctgactagg ctagcaatcg tatctggtaa gtctcttctt
    11761 ttgacaatag catccacaaa gccatgttct aataggaatt ctgccttttg gaaatcctca
    11821 ggcaagcttt cacgaaccgt attttcaatc acacgacgcc cagcaaaacc aaccaagctc
    11881 tgtggttcag ccagaatgat atcgccttcc atagcgaaag aagctgtcac accaccagtc
    11941 gttggatctg tcaaaatggt caggtaaaag agaccagcat ttgaatgacg tttaaccgcc
    12001 gcagagatct tagccatctg catgagactc atgattcctt cctgcatacg ggctccacca
    12061 gaggctgtga ataggacaac tggcaatttt tcgacagtcg catactcaaa caaacgagtg
    12121 attttttcac ctacaaccgt acccatagaa gccatgataa agttagaatc cataatccca
    12181 agagccacag tctgaccttt aataagagca gttcctgtca caacggcttc atgcagacct
    12241 gttttttcac gcatagatgc cagtttcttt tggtaaccag ggaaatgcaa gggatccttg
    12301 ctttcaatcc ctgtaaacaa ttctttgaag gttcccatat caatcgtcaa agccaagcgt
    12361 tcttgggcag aaatacgaaa ggtatagcta cagtgcggac agatacgttc acttcccaga
    12421 tccttctgat agatggtatg cttacagcct ggacactggg aaaataattc atctggaacc
    12481 tctggcttag cttgaggttt ttccctaact gaacgattgg gattgattcg aatatactta
    12541 tcttttttac taaatagagc cattgattcc ccttttcggt ttaaactctt aaagtcattt
    12601 tattcttttt cttgatattt aggtaagaag gtttccatca agaaggaagt atcataatcc
    12661 ccagcaatga cattgcgatc tgaaatgagg tcaagctgga aatctgcatt ggtctgcact
    12721 ccttcaatct ctaattcata gagggcacgt tgcattttca tcaaggcgtc aaaacgattt
    12781 tcgccgtgta ctatgatttt ggcaatcata ctatcataat aaggcggaat ggtataacct
    12841 ggataaactg ctgaatccac gcgcaagcca actccaccac ttggcagata gagattagta
    12901 atcttacctg gacttggagc aaagttaaag gctgggtttt ctgcattgat acgacactcg
    12961 atggcatgac cgcgtaggac aatatcttct tgcttaacag acaaaggctg acctgccgca
    13021 atgcaaatct gttccttaac gatatcaaca cctgaaacaa actctgttac tggatgttct
    13081 acctgaacac gagtattcat ctccatgaaa tagaaattgc tacttgcttc atcaagaaga
    13141 aattcaatgg ttcctgcatt ctcatagcca acaaactctg ccgctcgaac agcagcagca
    13201 cctatttcat gacgcagcgt ttttccgatt gcaatcgagg gactttcttc caaaaccttt
    13261 tggttattcc tttgaagaga acaatcccgt tcacccaagt gaatcacatg tccatgctca
    13321 tcacctagga tttgaacctc aatgtgccga gctggataga taacccgttc tatgtacatg
    13381 gcaccattgc cataattggc cttggcctca ctagaggcag tttcaaaggc agaaacgagg
    13441 tcatctggtt tttcaacctt acgaatccct ttaccacctc cacctgctga agccttgagc
    13501 ataacaggat agccaatttt ttcagcaaca atcaaagctt cttcagagtt atgcacttct
    13561 ccatctgaac ctggtataac aggcacacct gctttaatca tctgagcacg cgcattgatc
    13621 ttatccccca tcatatccat aacatgacca gatggaccga taaacttgat acctacttct
    13681 tcacacatgg tcgcaaattt ggaattttca ctgagaaatc caaaaccagg gtgaatagct
    13741 tctgcctcag tcaagactgc agctgataga actgcattaa tattgagata agactctgtt
    13801 gccttgccag gaccaataca aactgcttca tctgccaaaa gcgtatgaag agcttcctta
    13861 tcagcagttg aataaaccgc taccgtcgca atccccaatt cacgtgccgc acggataata
    13921 cgaaccgcaa tttcaccacg attggcaatt aaaatttttc gaaacatgga gaacctcctt
    13981 agttcccaat tgcaaaagta agggtaccac tggctgcaag cttgccatcc acttcagcct
    14041 ttgcttcaac cacagctatg gtgccacgac gttttacaaa agtcgctgtc ataaccaatt
    14101 ggtcgcctgg tacaacttgc ttcttgaact taaccttgtc cataccagcg taaaagacca
    14161 gttttccttt attttcaggt tttgataact ccaacacacc ggcagtttgc gccaaggctt
    14221 ccataatcag aacacctggc ataactgggt attgaggaaa gtggccgtta aagaaaggct
    14281 cgttgatggt cacatttttg atagcaacaa tggtatcctc gctcacttcc aagacacggt
    14341 ccactagaag cataggataa cggtggggaa gagcttcttt gattccttga atatcgatca
    14401 tttgatacgt accaatcctt taccaaactc aaccatttct tcgttagaga cgagaatttc
    14461 cgttaccaca ccatccttag gagctgggat ttcattcatg actttcatgg cttcgataat
    14521 taccaatgtt tgaccttttt tgacactatc accaactgta acgaaggcag gtttatctgg
    14581 tccagcagcc aagtaaacca ctccaacaag tggactctct acaagatttc cctcagtagc
    14641 cacacttgct tcagctggag ctggaacttc ttctgctaca gtctctgctg gagcagatgt
    14701 aggagctact ggactcggtg ttgctagaac gggtgctgga gcgacttgag ttgcaacttc
    14761 aggcacaggt ctcgcttcat tcttgctaaa ctgcaactca tccgtcccat ttttataaga
    14821 aaattctctc aaacttgact ggtcaaattg agtcatcaag tctttaatat cgtttaaatt
    14881 catacttatc tattctccca acgtttgaaa gcaagaactg cattgtggcc tccaaaacca
    14941 aaagtatttg aaatagcgta tggaatttct ttctccaagc cttgtccata aacgacatta
    15001 gcttcgatat aatctgatac ttcacttgtc ccagctgtca ttggtacaaa gttatgacgc
    15061 atagcttcga tggtgacgat agcttctact gcacccgcag cccccagcaa atgtcctgta
    15121 aaagacttgg ttgatgatac aggtacttcc ttaccaagaa cagctacgat agcaccactt
    15181 tctccttttt cattggcagg agttgacgtt ccgtgagcat tgacataggc tacttgctct
    15241 ggagaaatct cagcttcttc caaggctagt ttgatggcct tgatagctcc ctgaccttct
    15301 ggatgtggag aagtcatgtg gtaggcatca caagtatttc cgtaaccaac cacttcagcc
    15361 aggatagtag ctccacgttt ttcagcgtgt tcaagacttt ctagaaccaa catccctgaa
    15421 ccttcaccca taacaaaccc attgcgatcc ttatcaaatg ggatcgaagc acgagttgga
    15481 tcctctgtag tagagagagc tgttaaggct tggaaaccag cgatggcaaa aggtgtgata
    15541 gaagcttctg ttcctcccac caacatcaca tcttggaaac caaacttaat agagcggaag
    15601 gcatccccaa tcgcatcatt tgatgaagag caggcagtat tgatagattt acaaacacca
    15661 tttgcaccaa aacgcatggc tacattccca gaagccatat ttggtaaagc ttttggaaga
    15721 gtcattggtt tgacacgttt gggtcctttt tcatgaaggc gaagtacctg atcttcaatt
    15781 tccttgattc caccaatacc agatgcaacg ataacaccaa aacgatccct attaagagcc
    15841 tctacatcaa gattggcatg atttacagcc tcttgggctg catacaaggc atataaagaa
    15901 tagttatcaa aacggttggt atcttttttt acaaagtatt tatcgaacgg aaaatcttgg
    15961 atttctgccg cattatgcac atcaaagtca ctatgatcaa attttgtaat gccaccaatg
    16021 ccgattttcc cagttgctaa actattccaa aattcttctg gtgtatttcc gattggagat
    16081 gttactccat aacctgttac cactactcga tttagtttca ttcttttcac ctctagcttt
    16141 cgctacatac ttaagccacc atcaatggca accacttgac cagttagata atcttggcct
    16201 gctaaaaata ctgtcaaatc tgcaacctgc tctgcctgcc caaattcttt catcggaatc
    16261 tgagctagtg tagcttcctt aatcttatct gataagatag ctgtcatatc agactcaatc
    16321 attcctggag caatcacatt gactcgtata ttccgactag cgacctcgcg tgccacagac
    16381 ttggtaaagc caatcaagcc agccttagaa gcagcatagt tagcttgacc aatattcccc
    16441 atcaaaccaa caacactaga catattaatg atagcacctt ctctggcttt catcatcggt
    16501 ttcaagactg attgtgtcat attaaaggca ccagtcagat tgaccttgag cactttttca
    16561 aaatctgctt ctgtcatctt aagcataaga gtatcttggg tgatccctgc attgttgacc
    16621 aaaacatcta ctgaacccag ttctgcaata gcttgatcaa tcatacgctt agcgtctgca
    16681 aaatctgata catctcctga aatgggaacc accttgatac catagtttga aaactcagcg
    16741 agcaattctt ctgagattgc cccacgactg tttaagacaa tgttggctcc tacttgagca
    16801 aacttgtggg cgatggcaag accaattcca cgactcgaac ctgtaataaa gatattttta
    16861 tgttctagtt tcattttttt cctttcaaaa cttctactta ttttagtcta tttctctaaa
    16921 agtgctacta aactcgcttg atcttccaca tgagctaagt gagcagtttg atcaattttt
    16981 ttaacaaaac ctgacaagac tttccccggt ccaatctcga taaagttgct tatgcctgct
    17041 tcttgcatga ccccaatact ttcatagaaa cgaacgggtt ccttgacctg acgcgtcaag
    17101 agctgagcaa tgtcctcttt ttgcatcaca gcagcttctg tattgccgac taggggacaa
    17161 gtaaaatctg aaaaacttac ctgagctaga gtttcagcta gtttctggct agctggctca
    17221 aggagagcgg tgtgaaaggg acctgacacc ttaagaggaa tcaagcgttt ggcacctgct
    17281 tcttgcaaaa gttcaaccgc tcgatcaact gcaaccactt ctccagcaat gacgatttgt
    17341 gcaggtgtgt tatagttggc tggagtaacc actccaagtt cagaagcttt ttgacaggct
    17401 tcttcaatga cctctactgg cgtattgaga actgctacca tcttgccaga gtcagcagga
    17461 gccgcttctt ccatataggc tccacgctta gctaccaagg caaccgcatc ttcaaaatcc
    17521 aaggcgccgc ttgccaccaa ggcagagtat tctccaagag acaaaccagc aaccatatca
    17581 ggctgatagc ccttttcttg caataaacgg tagatagcaa ccgaagtcgc tagaatggct
    17641 ggttgcgtat agcgggtctg attgagtttg tcctcttccg tatcgatgag ataacgtaaa
    17701 tcataaccga gcacctgact cgctcgatca atcgtttctt tgacaatcgg atactgatca
    17761 tagaaatccc gtcccatccc tagatactgg gcaccttgac cagcaaataa aaaggctgtt
    17821 ttagtcattt cttacaactc ctgcccagcg agaggcttct tcttgaattt tcttagcggc
    17881 tccgtaatac aaatctttta ggatttcttc agctgtttct tctttagaaa caagtcctgc
    17941 gatttgacct gccatgacag agccaccctc cacatcaccg tgaacaactg ctttggctag
    18001 ggcacctgct cccatttgtt caaagatttc taaatcagga tcttcctgct taaaggcatc
    18061 tttttcagcc agttcaaaat ctctagtcaa ctgattttta atagcacgaa cagcatgacc
    18121 aaagtgctga gctgaaatcg tagtgtcaat atcccttgct tttaaaattt tctccttgta
    18181 gtttggatga gcattcgact cttttgcaac tacaaaccgt gtccccacct gtacagcctc
    18241 tgcacctagc ataaagccag ccgcagcacc ttcaccatcc gcaattcctc ctgcagcaat
    18301 aacaggaata gatacagctg tggctacctg tcgcaccaag gtcatggttg ttaatttacc
    18361 gatatgcccc ccagcttcca ttccttctgc aataacagcg tctgcaccga ttttttccat
    18421 gcgtttagct aaagcgacac taggaacaac aggaataacg attatcccag cttcatggaa
    18481 acgttccata tacttgcttg gatttcctgc tcctgttgtg acaactttaa caccttcttc
    18541 aataacgaga tccacgatat cttccacaaa gggagataag agcatgatgt tgaccccaaa
    18601 gggtttatca gtcaatgatt tgattttatc aatattggcc ttgacaactt ctttcggggc
    18661 atttccccca ccgataattc ctaatcctcc agccttggaa acagcccctg ccaaatcacc
    18721 atcagcaacc caggccatcc ctccttggaa aataggatag tcaatcttca ataattctgt
    18781 aatacgcgtt ttcatagtgc ctccaacctt ccttgcttac gtaatagttc gatttcacca
    18841 taatttgaca gtcaaactat tacctaaaca agagggagtg ggtttctccc tactccttct
    18901 actaatattc tgcttatttt tcttgctctt caacgtaagc aaccaagtca ccaactgttt
    18961 tcaagtcatt ttctgcttcg atttggatat caaaagcatc ttcgatttct gagattactt
    19021 ggaacaagtc caatgaatct gcgtccaaat catcaaaagt tgattcaagt gttacttctg
    19081 atgcgtcttt tccaagttct tcaacgataa tttcttgtac tttttcaaat actgccatga
    19141 taggactcct ttaaaataaa tagttttttt ataacaatgt gttcaccaca tgattaccta
    19201 aattgtaaga atgagcgtgc cccaggtcaa gcctccaccg aagcctgata gaagaacagt
    19261 ctggctacca tctaaaggga tgagaccttg ttctacacac tctgaaagta aaatcgggat
    19321 actggctgca ctggtattgc catattccat catattggct ggaagtttgg ctcggtcaac
    19381 accaattttt ctagccatct tatccaaaat acggtcattg gcttgatgaa gtagcagata
    19441 atccaagtct gtcacctcta taggagattc atcaatagtc tgcttgatag acttggctac
    19501 atctcgaatg gcaaaatcaa agactgtgcg tccatccatc ttcaaaaacg aatctgcact
    19561 ttcttgatct gaaaatggag aatgcaaacc tgaatgccca taagttaaac actcgctgcg
    19621 acttccatcg ctattgagac tctcagctaa gaaatgctct tgctcgctag cttctaacaa
    19681 gacaccacca gcaccatctc caaacaacac agctgttgat cgatccgacc aatcgactgc
    19741 cttagagagg gtttcactac caatcaccaa gcctttttga aagcgaccag aagcgataaa
    19801 cttttcagca gttgaaagag caaatacaaa tccactgcaa gccgcggtta agtcaaaagc
    19861 aaaggcttta ttagcgccaa tattagcttg aacacgagca gctgtagagg gcatcatcga
    19921 atctggagta atggtagcta ggatgataaa atccagttct tttcctgtta ttccagcttt
    19981 tgccatcagt ttcttagcaa cctctgtagc caaatcactg gtagattctg ttcttgaaat
    20041 atgcctttgt cgtattcccg ttcgacttga aatccactca tcattggtat ccataatctg
    20101 agccaagtcg tgatttgtaa ccacttgctc tggcacataa tgagcaacct gacttatttt
    20161 tgcaaaagcc attatttcaa atcctccaaa aattggtaaa gattagtcaa gcctttaccc
    20221 atgacagcaa tttcttcctc gctcatgcca tcaataattt tttctaccat ggccttgtgg
    20281 aagcgtttat gcagtctatg aatcaagcga cccttctttg tcaaatgcag atgcaccaca
    20341 cgacgatcct gttctgaccg aactcgctca atgtagccct tacgctctaa attgttcaaa
    20401 cttgtcgtaa cagttccaag agttaccatc aactcttttg acacttgact tggagtcaca
    20461 tctggagcct taccgatgac atcaatcgta tgcatttctt tgatggagat atccttaaaa
    20521 cgactacctc tcaagttcac ttcctcaatt acaaggacat tgttaaatat agatgttaaa
    20581 tattcattga ttcgttggta gtccattttt catatccctc cttcttcaaa aaacttttac
    20641 aatcaaaaca tttgacagaa agaattatat caaaattcaa agaatgatgc aagtattttt
    20701 tattttcctg taaatttagg tcttcttctc tccgaatgag ctcgcactcc ttctttgaaa
    20761 tcctctgttt gagctaagga tttctgtagg ttcagttcta aagtagcgta accctgccag
    20821 tctttaaatt ggctctccca aaccaacttc ttaatggcag cataggaatt acttgaagca
    20881 cgtcttaatt ttttaagaag ctgttctctc gtcttttcaa gtttttcagc ttcagaaacg
    20941 cggtaaacca acccccattc tagggctttt tctgccgtta aagcctctcc tgtcatagct
    21001 aattgagcag cacgcgtcac accgatactg cggctcaaga gatgaattcc ccctgcatct
    21061 ggagccaaac caacaccaac aaaggcttgg ataaacttag ccttatccgt cgccaaacag
    21121 aaatctgccg caacagccat attcgctgcg gcacctgcaa cagcaccgtc aacttccatt
    21181 aaaacaggtt tggctatttg cttgatttta taagaaatcg tattgaccaa ctctgcgatt
    21241 tttgtcaatg atggaatatc atcctcatcc actgcccgct tcatctctac caaatctccc
    21301 ccaactgaga agacctttcc attggcattg attaagataa aatgcacagc tggattttct
    21361 tctgccaaag tcagagcttc taaaatctcc tcacacatgg gaatatgaaa accatttgcg
    21421 acctcaggac ggttcaaggt aaggattgcc aagtcctctt caagctgata aataatgtgt
    21481 tccatcaggg ctccttttta ttttataagc agtttatact cttcgaaaat ctctttaaac
    21541 cgcgtcagct tccatctgca acctcaaaac agtattttga gctgacttcg gctagcttcc
    21601 tagtttgctc tttgattttc attgagtgtt aaattatttc actgtcaaag tatatctttt
    21661 ttaggagcag aaagcaagaa aaaatcgact gaaagtgtct cagccgataa gaaaggctcg
    21721 ctttaggcta gaaagcatct tattttcttc attgaaaaaa gcctttcttt ctgctataat
    21781 cgtataaaat acttacttta ggagttctta tgaaagttgt taaatttgga ggtagttctc
    21841 ttgcctctgc tagtcaatta gaaaaagttt taaacatcgt caaaagcgat tcagagcgtc
    21901 gttttgtagt cgtttctgcg cctggtaaac gcaatgctga agatactaag gttacggatg
    21961 ccctgattaa atactaccgc gactatgttg cgggtaacga tattagcaag agccaaagct
    22021 ggattatcga ccgctatgct gctatggtta gtgaattggg actaaaacca gctgtgctag
    22081 aaaaaatttc taaaagcatt cacgccttgg ccactcttcc tattgaagaa aatgaatttc
    22141 tctacgatac tttcctagca gccggtgaaa ataacaatgc caaattgatt gctgcctact
    22201 ttaaccaaaa tggtatcgat gcacgctata tgcaccctag agaagctggg attgtggtca
    22261 caagtgaacc tggtcacgct cgcatcattc catcaagtta tgacaagatt gaagaattga
    22321 caaacaccaa tgaagtcctt gtcattcctg gtttctttgg tgtcactaag gaaaatcaaa
    22381 tctgtacttt ctcacgtgga ggttctgata ttacaggttc tatcattgct gctggtgtca
    22441 aagctgacct ctatgaaaac tttacggacg ttgatggtat ctttgcagcc caccctggta
    22501 ttatccacca accacactcg attcctgagt tgacctaccg tgaaatgcgc gagttggcct
    22561 atgcaggctt ctcagtcctt catgacgagg ctcttcttcc tgcctaccgt ggaaaaattc
    22621 ctctggttat caagaatacc aacaaccctg accatccagg tactcgtatc gttctaaaac
    22681 acagtaatga tgaatttcca gttgtgggaa ttgctggtga ctcaggcttt gtcagcatta
    22741 acatgtcgaa atacctcatg aaccgtgagg ttggatttgg ccgcaaggtt ctgcaaatcc
    22801 tggaagaact taacatcggt tgggaacata tgccaacagg tatcgacgat ctttctatca
    22861 ttctccgttc tcgccaacta actcccatca aggaagaaga aatcctgcgt cagttggttc
    22921 aaaaggctaa agtagatcat gcagaaatcg aacacgacct ttctatcatt atgattgttg
    22981 gtgaaaaaat gaagagccac atcggagtga ctgctactgc gacacgcgct ctatctgaaa
    23041 acaaaatcaa catccagatg atgtctcaag gttctagtga agtttctatt atgtttgttg
    23101 tcaataaaga ccaagagaaa gcagcaatta aagccctcta caatgccttt tttggagaaa
    23161 gtaaggaaga ctaagcatgg cccaatcact taacaaaaca gtactcctca gtacgacagg
    23221 cacttcctac ctctctatcg ctgggaaagt tgggaaattc cttgtcggag atcaggcttt
    23281 ggaattttac ccagatgtca atgtcgaaca atttatccag attccttgga gccatatcaa
    23341 tcagattgga gccaatgtca ctggatgcaa aatcagccgc cacttcgaag tctttacaga
    23401 caaaggaaaa ttcctctttg cttcaaaaga ctcaggtgcc attctcaaaa ttgcacgcga
    23461 caaattgggc aatgacaagg tcgtcaaact tccgaccctg attcagacaa ttagccaaaa
    23521 atttaaaaat ctatttgcaa aaaaatagaa actttagtat aatcatagca acggataagt
    23581 aggttatctt cttctttcag aaagtctgcg ggtgctgcga gcagatagga gggataggtg
    23641 aaattctacc gttagtaaca attacataca caatcaagtg cacacctgtg aagttggatg
    23701 gaaccgtggc cctgccactc caacgttttg tcaggtgtgc ttttttcata aaggagttct
    23761 tatgttagat atcaaacgta ttcgtacaga ttttgaagct gtcgcagaaa aattagctac
    23821 acgtggtgta gatgctgctg tcttgaatga aatgaaagaa atcgatgcta aacgtcgtaa
    23881 catcttggtc aaggttgaaa ctctcaaagc agaacgtaac acagtttctg ctgagattgc
    23941 ccaagctaag cgcaacaagg aaaatacaga tgacaagatt gctgccatgc aaaatctatc
    24001 tgctgaggtt aaagccttgg atgctgaatt ggcagaaatc gatgctaaat tgacagaatt
    24061 tacaacgact cttccaaata tcccagctga cagcgttcct gttggggctg acgaagacga
    24121 caatgtggaa gttcgccgtt ggggtactcc acgcgagttt gacttcgaac ctaaagctca
    24181 ctgggatctc ggtgaagacc ttggtatcct tgactgggaa cgcggtggta aggtaacagg
    24241 cgctcgcttc ctcttctata aaggcctcgg tgctcgtttg gaacgtgcta tctacaactt
    24301 tatgttggat gaacatggaa aagagggcta tactgaagtc atcacacctt acatagtcaa
    24361 ccatgattct atgtttggta ctggtcagta tccaaaattt aaggaagata cttttgaact
    24421 cagcgatacc aactttgtct tgattccaac tgctgaagtt cctctggcaa actactaccg
    24481 tgatgaaatc ttagacggca aagatcttcc aatctacttc actgctatga gtccgtcatt
    24541 ccgttctgag gctggttctg ccggtcgtga tacgcgtggc ttgatccgtt tgcaccaatt
    24601 ccacaaggtt gaaatggtca aatttgccaa accagaagaa tcttacgaag aattggaaaa
    24661 aatgacagcc aacgctgaaa acattcttca aaaactcaac cttccatacc gtgtcgttgc
    24721 tctctctact ggagatatgg gcttctcagc tgcgaagact tacgacttgg aagtgtggat
    24781 tccagcacaa aacaattacc gtgaaatctc aagctgttca aacacagaag atttccaagc
    24841 ccgtcgtgcc caaatccgtt accgtgatga agcagatggc aaggtgaaac tccttcatac
    24901 cttgaacggt tctggacttg cagttggacg tacagtggct gcaattcttg aaaattacca
    24961 aaatgaagat ggttctgtga ccatcccaga agcacttcgt ccatacatag gtggagctga
    25021 agtcatcaaa ccataaaaaa taaggtttag ctatttctag ctagaccttt tttcgtaacc
    25081 aaatcagata agcacctagt acaaagaata aaatagttag gcatataatg gtttcagcca
    25141 ataccaggta atccagaaat ggaagtttca aaattccctg agccatcttg agcgaggtcg
    25201 ctgtgataat ggttgggaag gtgagggctg agaaggctgg ttgaaagcct tgttttaaaa
    25261 tgttgggcat acgagttaaa acaaagaaaa agaaggattg agaagccaaa atcatgacaa
    25321 tcaagcccca agtcggcagg ctggttcctc ctactcgaac tagagaagcc aagagtagag
    25381 agaaaggagc acagtagatt ccttcttgtc caagcaaggc tagtgggagt ggatgtttct
    25441 ttaaatcgct ataaataagg ggatagagat agaaggtcaa gagaaaacca aaactcaagg
    25501 tcgcataggc aatttcgata atacctacca gaggataggt caaggcagcc actgctatcc
    25561 ccacatagag aaccgtccaa cttggagtgg catgaaccct ccgccctgga caagcaaact
    25621 tgatggtaaa accagcaatc aaggtcaaat ccaagagaaa tgaaaaccac caaatccctt
    25681 gtgctaccaa aggaagataa gagaatacgc gaaagacata ggtcgataaa atcatcccag
    25741 ccataggaaa ggttgccatt cctgacaaaa gagggggctt ggtcaattct tgcttggttt
    25801 ctttccaatt aaagagatgc agaattagaa agtaaatcca taaaaccaaa ccagtcagac
    25861 taaaaagatg ggatagaacc ggcaacgtat ctaaaataag atttccagct cctgccaaac
    25921 ctagcaaaca acctgaaaat actaagggga gttttttcat cctaacctcc aataatcatg
    25981 ttagtttcag tataacataa aagcgcttaa atgaggattt aaaaaaacga agtccgctta
    26041 tttcagactt cattttactc agatatgaat taggcataag gttgcaattc tggattaatt
    26101 ggtgtattag ctaagttgtt ggcatagtta cagaggattg ctaggctgac accaaaaacc
    26161 acatccaagg cattttgttg agtgtagcca gcttctaaaa actcagacaa ggcttcatct
    26221 cctacacgac ccttggtatt gataactgcc aaggtaaact tagctagggt atccaattta
    26281 ggatctgttt caattggagt acgattgcga agagcttgaa tcaagtcatc attcatctgg
    26341 atttgtttga tggaaaaggc tgtgtgacct gcgacacaga aggcacaacc attggtcacg
    26401 gctgccgtga tttgcaccac ttcacgctca acgggtgtca ggctgttgcg acggtggata
    26461 gatgagacaa tttggtaggc ttctaaaaca gtcggggcat tggccaagag accgattagg
    26521 ttgggaatat agccattgtt gtctttttct actgtttcaa gaatttcttt cacttctgct
    26581 ggtgctgact ctactgtatg gatagtaaat gttgtcataa gatacctctt ttcttattat
    26641 tgacactaat attattggaa aatcttataa aatcctgatt cctaagttta tctaagataa
    26701 agctttattc tctcataaga ttttcgttgt tatattagtt tatcacactt ccaatcactt
    26761 gcataatata tattatatat caggctgata aaaattattt ataggcaaaa aaatcacacg
    26821 agctgtgtga ttccattatt tgtcaaaata ctttttagtt tcagcaataa cgactggcga
    26881 caagaccaag agggcaatca agtttggcag agccatcaag gcgttaacga tatctgcgat
    26941 aatccagacc atatccaact cgataaatcc tcctaacaag accatgagca caaaaaccac
    27001 acggtagagc cagataaagc gaaccccaaa gaggaactca aaacagcgtt ctccgtaata
    27061 gttccaacct agaatcgttg taaaggcaaa aagcacaagg aagatggtca agagggcagg
    27121 gccaaagtgt gaaaagactg ttgagaaagc tgactgagtc aaggcaaccc cattcaagtc
    27181 accactccaa actccagtta ccaagatggt caaaccagtt agagtacaaa tgatgagggt
    27241 atcaataaag gttcctgtca tggaaatcaa accttgctct actggttcat ttgtcttggc
    27301 agctgcagct gcaataggag cagaacccag accagattcg tttgagaaca caccacgcgc
    27361 cacaccattt tgaatagcca tccgaacgct agcaccagca aatccaccta ccgcagcaag
    27421 gggactaaaa gctgaggtaa agactaaagc gattgtgcca gggatttttc cgatattaaa
    27481 gaaaataact gtaagagttc ctaagatata aatgagggcc ataaaaggaa caacagtagt
    27541 tgaaacctta gaaatagact tgagtccacc aaagactgca atcgctacaa agacagacaa
    27601 gacgagagct gtgatggctg gcgaaatcgt cgttgtattt tggatagatt ctgtaatcga
    27661 gttgacttgg gtgaaggttc cgattcccaa gagagcaacc aatactcctg ctactgcaaa
    27721 caaaacagca agtggtcgcc acttttctcc catccctaga aggatatagt gcatgggacc
    27781 tcccgctact gcaccatggt cgtccttggt gcggtatttg atggccaaga gtccttccgc
    27841 atacttggta gccattccaa agaaagccgc catccacatc caaaatagag ctcctggtcc
    27901 accaaccttg atagccgtcg caactcctat gatatttcct gttccaacag ttgatgccaa
    27961 ggctgtacac agagctgcaa aactggatac atcaccatgt cccttatcct ggataaaaat
    28021 aagctgaaag gccttgggca gacgcaaaac ctgcaagagt cctagccgca tggttaggta
    28081 aatccctgtt ccgaccaata aaatcaagag gggcggtccc caagcaaaat catcgattga
    28141 tttaagcaat tctaacattt ccttctccta tcgtttcaac cccaaaagaa agagcacatg
    28201 caagatacat gtactctgga atgcttagat aaatgctaaa aagcggtcta tcctagctct
    28261 gtccttttac ctgagagttt gagcagttgc ctgccttgcc ccttcggtgc ctttacggtc
    28321 tctccagagt tccgtccatt tacagtcatg gaaaatcaaa cgattcccca cttctattaa
    28381 acttcattcg gtgttggtat ttaattgatt ctaattttac aaaaaatgtt ggcttttgtc
    28441 aatgtgttta ttagtaaaaa ttagttcaac agtttttact ttataaagtc cagaatactg
    28501 ctatccttta aaagtgacaa tagtcgcacc actgcctcca gcattttgtg gggcatagcc
    28561 gaaactcttg acatgtttgt ttctttgcaa gtatttggta actccttcac ggatgactcc
    28621 tgttccgata ccatggatga tatcaacttg agccatattg ttaagcaagg cttggtcgat
    28681 gaaggtatct agctcattca tggcttcttc atagcgcttg cctcgaagat ccagtctagc
    28741 ttgaggtcct cgcccagaag ttcgtttcac aacattgacc tgtttcttct tgactggttt
    28801 ttcttgctgg gcttgaacaa gatcaaactc tttctcttcc aaggtcatct taatcaagcc
    28861 aacttgggct tcccagcggc cgtccttgag ttgactggtc aaggtaccac gctgaccata
    28921 actgagaacc acgatatcat ctcccacctt tggagctcgt tttttcttgg ccttttgaag
    28981 gaccttgttt ttagacaagt ccactttttc aggagccaat tttttcaact tggccttggc
    29041 ttcaatgatt tcgtggggct tgagttggga tttactgtgg agatttttga gaatctggtc
    29101 actttcactt agggccatat ccacaatctc agcagcctgt tcacgcgcct tgttaagctc
    29161 ggtttccttt tcacgattaa gctcgttgta gagttttttt agcgcacggt tcatcttgag
    29221 attttcttgc tccacctcac ggatattgtc caaacgtttg cggctttcca gcgtctgctc
    29281 ttctaattgc tcaatgatac gattgacgtc attgtcctga tcgatctgct gactggcatc
    29341 tcctacgata acttcagata ggcctagacg tttggcaatt tcaaaggcat tacttcggcc
    29401 aggaacaccc tgcataaagc gataggtcgg gcgaagagtt gcagtatcaa actccatact
    29461 ggcattttgc acaaaggctg tctcaatacc gtaggccttg agttctggat agtgggtcgt
    29521 cgccatggtc ttgatttgac gcaggcgaag gtcctccaga atagccatgg caagggctgc
    29581 tccctcttgg ggatcagtac cagcccccaa ctcatccaaa agtaagagtg aatgttggtt
    29641 gaccttgcca agaatatcca cgatattggt catatggcta gagaaggtag acaagctctg
    29701 ctcaatagac tgctcatctc caatatcagc aaagatttct tcaaaaatac caacacgact
    29761 tcccttgtct gctaaaatcg gcaatcctga ctgggccatg acctgtgtca agcccagagt
    29821 tttgagcatg atggtcttcc cacctgtatt gggacctgta atgacaatag ctgttaaatc
    29881 ttgaccaaaa tagacatcat ttgcgacggc atttttgacc aaaggatggc agacatggag
    29941 cagttgaatc tcttgatttt ctgacagctg aggcacgact gcttgtcttt cttggataaa
    30001 tcgaaccttg gcacgaatca agtccagatg accgataatc caagcgtcat tagcaatctc
    30061 agccgcatga gggcggacac gctcagaaat ttcttggaga atgcgaagca tttcatagcg
    30121 ctcatctgct cgcagactag caatttcttc gctcagtttg actacctcac gtggttcgat
    30181 atagacggtg tttccactag cagaaatatc atgaacgaca cctgcaatct tattgcggta
    30241 ggtgtttttg actggtaaaa cctgacggcc atttctgcta gcaacaattc cttccgtcaa
    30301 catctgcgct ttttgcttga gtaagtcttg taaaacatcg cgcacctgac tctcgctatc
    30361 atgtattttt cgacggattc gcgccaattc ttcactggca aaattttcaa tgaaacccgc
    30421 atcattaaag gcctgaagat ttccttgtaa ttgcggaaaa tcatgtaatt tctcaaacca
    30481 aagggctaat tcttccaagc tgacattttc cagattggcg taaaaatttt gaagttctcg
    30541 gctggcaaga agcacgcgtt tcaagagtag gaactcctcg atattgagat ccgctcccat
    30601 ctccaacctc ttgcagactc ctgcaatttc cttagttgag agaatagtaa aatgcggttg
    30661 ctcgacgaaa agagcctgca tttctttcat ctcagcaaaa gcctgtttga ttttatctgc
    30721 tttggcagtc ggagccagct gtctcaattg ctccaagccc tgctcggtca acaaatgagg
    30781 ctcaaacaag gccttgacct tatcgaactc taatgtttct aatattttct tattcatctt
    30841 tatttccttg tctttgaatg tctaggtgtg gtagcttttt acattctgat agattcgagt
    30901 caaatctgta aacaaagggc taggaaaact cctgcccttt ttatccgatt aaatttgtca
    30961 cccagatttg tttgagccaa ctggttgtta tcggtatgct ctggatgatg tgttttgcga
    31021 cgatactctt ttcaagagga ttttgtataa ctgccatggg gatggtcgcc aagattgtca
    31081 aggccatttg caagacaaat aaggtcacca acatggacaa gatacctgct gaaacttgga
    31141 acaacttacc acccagtttt ttgctaggaa tcaagtgtaa gagaagacca agcaaacgac
    31201 caatgctata gacaatccca aatacaagca agtagccgat acctgcataa aagaccttat
    31261 ccagctgaaa gagttgatcc gatgggaaaa agaaagtccc ctgaccttcc tgcgaatttg
    31321 cataagggag caataaatgg aattgctctc caagcccctt ataaaactgg ccagccataa
    31381 aagccgatgc catggctgaa atcaggtaat aaacctgtaa gagcaggcct ctccgatagc
    31441 cgatataaaa tccccaaacc aagaccaata gaagaaggag tgaaatcata aggaatcctc
    31501 aatcttgctc tgttcttgct tacaagtcac aagcttgtga cggagttctt ctagctcttg
    31561 ctccttatcg tcaaattcaa tctcacggct gagctgagtt gataaacagt tgactgccaa
    31621 caaaagagcg attgtttcat catctgtgct aggcatttgt tctttaattg cttggtattt
    31681 ttctgtcgca accttagcga tttcctccat aaaaaggtta tcatgttcgc ttgtcaaggt
    31741 taacgatttt ttcccgaatg taaatttgaa tcgatttaga tttgccataa aattcacctc
    31801 acgatattat accaaaattc gctaattttg tcagttttta caaattttac tgcttttgtg
    31861 gtacaataga aactatggca agtatcacac tcacaccaag cgaaaaggat attcaggctt
    31921 ttcttgaaca ctatcaaacc agtctggctc ctagcaagaa tccctatatc cgctactttt
    31981 tgaaactacc tcaagcaacg gtttctatct atacttctgg aaaaatcttg cttcagggtg
    32041 aaggggctga aaaatacgcc agtttctttg gctatcaagc tgtagagcaa accagcggac
    32101 aaaatcttcc tttgattggg acagatgagg tagggaatgg ttcctacttt ggtgggcttg
    32161 cagttgtggc ttcctttgtc acacctgacc agcatgcctt cttacgaaaa ctcggtgtgg
    32221 gagattctaa gactctgacc gaccaaaaga ttcgtcagat tgctcctatt ctcaaggaaa
    32281 aaattcagca ccaggcactc cttctctcac ccagcaagta caacgaggtc atcggagacc
    32341 gctacaatgc tgtttcggtt aaggttgccc tccataatca ggctatctat ctcctccttc
    32401 aaaaaggtgt tcagcctgag aaaattgtga ttgatgcctt taccagtgct aaaaattatg
    32461 acaagtactt ggcacaagag accaatcgtt tcagcaatcc tatcagctta gaagaaaagg
    32521 ctgagggcaa atacttggct gtcgcagttt cttctgtcat tgcgcgtgat ctctttctgg
    32581 aaaatcttga aaatttggga cgagaactgg gttatcagct cccaagtgga gctggaacgg
    32641 cttctgacaa ggtggctagc cagattttgc aagcctatgg tatgcaggga ctcagcttct
    32701 gcgccaaatt gcactttaaa aacactgaaa aagcgaaaaa acgcttagaa aggtaagtta
    32761 tgaattcatt taaaaatttc ttaaaagagt ggggattgtt cctcctaatt ctgtcattac
    32821 tagctttaag tcgtatcttt ttttggagca atgttcgcgt agaaggacat tccatggatc
    32881 cgaccctagc ggatggcgaa attctcttcg ttgtaaaaca ccttcctatt gaccgttttg
    32941 atatcgtggt tgcccatgag gaagatggca ataaggacat cgtcaagcgc gtgattggaa
    33001 tgcctggcga caccattcgt tacgaaaatg ataaacttta catcaatgac aaagaaacgg
    33061 acgagcctta tctagcagac tatatcaaac gcttcaagga tgacaaactc caaagcactt
    33121 actcaggcaa gggctttgaa ggaaataaag gaactttctt tagaagtatc gctcaaaaag
    33181 cccaagcctt cacagttgat gtcaactaca acaccaactt tagctttact gttccagaag
    33241 gagaatacct tctcctcgga gatgaccgct tggtttcgag cgacagccgc cacgtaggta
    33301 ccttcaaagc aaaagatatc acaggggaag ctaaattccg cttctggcca atcacccgta
    33361 tcggaacatt ttaagagacc taagaggccg agaatcacca atctcagcct cttcttctat
    33421 cgtgagaagt gattgtttat tgacaaatac tcaatgaaaa tcaaagaaca aactaggaag
    33481 ctagccgtag gctgcttaaa acagtgtttt gaggttgtgg atagaactga cgaagtcagc
    33541 tcaaaatact gttttgaggt tgcagatgga agctgacgtg gtttgaagat attttcgaag
    33601 agtataaaat aaaaacaatc ccaactctca cctattcatg caaaggaatt ttatggaagt
    33661 ttatttttca ggaaccattg aacggattat ttttgaaaat cccagcaatt tttatcgcat
    33721 cctcctccta gaaatcgacg atacggacgc agaggatttt gatgactttg aaatcattgt
    33781 cacaggaacc atggctgatg taattgaggg cgaagactat actttttggg ggcaaattgt
    33841 ccagcactcc aagtatggag aacaactgca aatcagtcgt tatgatcgcg caaaaccaac
    33901 tagtaagggc ttggtcaagt acttttcaag tagccatttc aaggggattg gtctcaagac
    33961 agctcagaaa atcgtggata cctatggcga aaataccatt gacgaaattt tgcaacaccc
    34021 agaaaagtta gaaggcatcg caggactctc tgccaaaaat cgcgaggctt tcgtctccac
    34081 tctccgtctc aactacggaa cggagatggt tttggccaaa ctagccaact acggcattcc
    34141 caacaaacta gcctttcaga ttcaagactt ttacaaggaa gaaacccttg atgtggttga
    34201 aaattatccc taccagctgg ttgaggatat caagggtttg ggctttacca ttgctgacca
    34261 actagctgag gaactaggca tcgaaagtca ggctcctgaa cgcttccgcg ccggtctagt
    34321 tcacagtctt tttcaggcct gtatggaaac aggggacacc tatgttgaag cacgggattt
    34381 gctggaacaa acccttactc tccttgagtc ttcccgtccc gtagaactag accccagcca
    34441 agttgcccaa gagctctcct acctgatcga agaagacaag gttcagcaga ttgataccaa
    34501 gatctttgac aacagcctct ttttcgctga ggaaggcatc cgcagtcact tggttcgtat
    34561 ccttgaaaaa ggaaaacaga agagtcatga tttagaaacc attcaaaaac atatcactac
    34621 tatcgaggaa gagctgggaa ttgagtatga taacattcaa aaacaggcta tctgtgatgc
    34681 tatccagaat aaggtcttta tcctgacagg tggacctggt actggtaaga caactgttat
    34741 caatgggatc atcgctgttt acgccctgtt agagggactt gacctcagga agaaaagcaa
    34801 tctgcccatt cttcttgctg ctccaactgg acgagccgct cgccgcatga atgaattgac
    34861 aggtttgcct agcgcgacca tacaccgcca cttgggaatg acaggtgacg atgataccag
    34921 tcatctggaa gattacctag atgctgactt tatcatcgtg gatgaattct ctatggtgga
    34981 tacttggctg gccaatcaac tcttctccaa tatctcttct aacagtaaga tcctcatcgt
    35041 aggtgacagt gatcagctac cttctgtcag tcctggacag gttctagcgg atctgcttca
    35101 tattcctttg attcctcaga ctcgcttgga aaaaatttat cgacaaagcg aagaatcaac
    35161 catcgtcacc ctagctagtc agattcgaca gggcatcttg ccagctgatt tcacccaaaa
    35221 aaaagctgac cgttcctact ttgaaattgc tagtggccat attcctgcca ccattgaaaa
    35281 aatcttaggt gccgccctca gaaatggtat tcctgcccgt gatatccaag ttctggctcc
    35341 tatgtaccga gggacggcag ggattgatgc tatcaatcag ctcatgcaag acctgctcaa
    35401 tcccccacaa aaagatcaac tcagttttga agttcctcag tgccactatc gtaagggaga
    35461 caaggtcatt catttggtta acgatgctga aatcaatgtc tttaatgggg atttaggagc
    35521 tatcacagac ctgattcctg gtaaatacac cgaatcgaaa caagacgaga ttgtcattga
    35581 ttttgatggt aatgaagtct cttacccccg taacgaatgg tacaagattc gcttggccta
    35641 tgccatgagt attcataagt ctcagggaag tgagttccca gttgtcatcc taccgattac
    35701 tagtgctagc cgacgtatgc tggagcgaaa tctcatctac acagccatta cacgtgccaa
    35761 aagcaaactc atcttactag gcgaattaca agccttcgac tatgctaccc aacacatcgg
    35821 aactgcccga aaaacctatc tgattgaacg cttcagtgat ctactggaga atgttgaaga
    35881 aaagcaacaa gctgtctctg aaacagtcac atcaagtgcc tctgaacaat cctacatcct
    35941 aaccgaagaa aactgggaca gcattccagc catgattggg attacagaca cagacctcaa
    36001 agagattttt ggaaaatagt gcatcagaaa aaccaccaat tcaggtgggt tttctgttta
    36061 ttaagattat tttactgttg ctgtgcttgt aatcaattca acagcttttt tgatagtgat
    36121 atcatgtttc aacatgtcag ctgaaagcaa gttttgaact tgtgcaactt ccatgttgta
    36181 gtctgctgcc aattgctcaa cttctttttg gatttcttct tctgaagcat caaatccttc
    36241 agctttggca actgcttcga taacaaggtt agtcttagta cgtgactcag cttctgcttg
    36301 gtattggttg tgaaggtctt cttgagtagt tccagtgatt tggaagtaca tgtcagggtt
    36361 gatcccttga cgttgcaagt tcccaaggaa ttcatttact gaacggtgaa cttcttcgtg
    36421 gatcatttct tctggaagtt ctacgatttc agcattttct acagctgtat caattgctgc
    36481 accttcaact gcatctttgt aagcttcttc tttagcagca gccaattctt tgcggtattt
    36541 ttctttcaag tcagcaagtg tttcaacttc ttcatcaatg tcttttgcaa gttcatcgtc
    36601 aagagctgga acttctttag cttttacttc gtggatagtt gtcacgaatt tagcttcttt
    36661 acctgcaagg tcttctgctt ggtagtcttc tgggaatgtt acgataacat caacggtttc
    36721 gccagctgag tgacctacca attggtcttc gaaaccaggg atgaattgac ctgaaccaag
    36781 tccaagtgag aagttttcac cttttccacc gtcaaattca acaccgtcga tagaaccaac
    36841 gaagtcgatc acaacagtgt cgccgttttc agcagcagct tccttgataa ccaattcagc
    36901 caggttgttg cgttcgcgtt cgatacgctc ttcaacatca gcgtcagtta cttctttttc
    36961 tacatcaact gatacttcaa ggtttttgta gtcacccaat tttacttcag gttttgtaac
    37021 gacttcagca gcgataaccc agtcttgacc tttttccatt gaagttacgt caatttttgg
    37081 ttgggcaacc acttcaagac cagcttcttt tacagctgct tcataagcgt ttggcaaaag
    37141 agcgttcata acgtcttggt aaagtgattc ttcaccaaat tttttatcga agataggacg
    37201 tggaaggtga cctttacgga aacctggaac attaagagat ttcttcactg acttgaagac
    37261 acggtccaat tctggtttga tttggtcttg agagatagtg aaagtcaaga caccacggtt
    37321 tgtttctttg ttttcaaatg atacagacat tctgtcattt ctccttaaaa ttttttaaat
    37381 acagtctatt ataacatttt ctataaataa aatctagact aatgcagcca attatagaca
    37441 acaagccttt ttcctaacta aaa
//
LOCUS       DAVMHG010000004        37291 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMHG010000004 DAVMHG010000000
VERSION     DAVMHG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.004"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(653..1066)
                     /gene="ndk"
                     /locus_tag="VYR08_000153"
     CDS             complement(653..1066)
                     /gene="ndk"
                     /locus_tag="VYR08_000153"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV3465744.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(1177..4854)
                     /gene="rpoC"
                     /locus_tag="VYR08_000154"
     CDS             complement(1177..4854)
                     /gene="rpoC"
                     /locus_tag="VYR08_000154"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV3465745.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4887..8498)
                     /gene="rpoB"
                     /locus_tag="VYR08_000155"
     CDS             complement(4887..8498)
                     /gene="rpoB"
                     /locus_tag="VYR08_000155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3465746.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8914..8984
                     /locus_tag="VYR08_000156"
     tRNA            8914..8984
                     /locus_tag="VYR08_000156"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8946..8948,aa:Cys,seq:gca)
     gene            complement(9182..10513)
                     /locus_tag="VYR08_000157"
     CDS             complement(9182..10513)
                     /locus_tag="VYR08_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV3465747.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10804..11628)
                     /gene="endA"
                     /locus_tag="VYR08_000158"
     CDS             complement(10804..11628)
                     /gene="endA"
                     /locus_tag="VYR08_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV3465748.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQTLAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSNATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11667..11855)
                     /locus_tag="VYR08_000159"
     CDS             complement(11667..11855)
                     /locus_tag="VYR08_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV3465749.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11848..13131)
                     /gene="murA"
                     /locus_tag="VYR08_000160"
     CDS             complement(11848..13131)
                     /gene="murA"
                     /locus_tag="VYR08_000160"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3465750.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGIRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(13155..13385)
                     /locus_tag="VYR08_000161"
     CDS             complement(13155..13385)
                     /locus_tag="VYR08_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV3465751.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13443..14480)
                     /locus_tag="VYR08_000162"
     CDS             complement(13443..14480)
                     /locus_tag="VYR08_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV3465752.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14464..14952)
                     /gene="coaD"
                     /locus_tag="VYR08_000163"
     CDS             complement(14464..14952)
                     /gene="coaD"
                     /locus_tag="VYR08_000163"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV3465753.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(14942..15481)
                     /gene="rsmD"
                     /locus_tag="VYR08_000164"
     CDS             complement(14942..15481)
                     /gene="rsmD"
                     /locus_tag="VYR08_000164"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV3465754.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15546..16538)
                     /locus_tag="VYR08_000165"
     CDS             complement(15546..16538)
                     /locus_tag="VYR08_000165"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV3465755.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16650..17333)
                     /locus_tag="VYR08_000166"
     CDS             complement(16650..17333)
                     /locus_tag="VYR08_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV3465756.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(17389..18129)
                     /locus_tag="VYR08_000167"
     CDS             complement(17389..18129)
                     /locus_tag="VYR08_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV3465757.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18163..18441
                     /locus_tag="VYR08_000168"
     CDS             18163..18441
                     /locus_tag="VYR08_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV3465758.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18519..19445
                     /gene="yidC"
                     /locus_tag="VYR08_000169"
     CDS             18519..19445
                     /gene="yidC"
                     /locus_tag="VYR08_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV3465759.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19520..20314)
                     /gene="pflA"
                     /locus_tag="VYR08_000170"
     CDS             complement(19520..20314)
                     /gene="pflA"
                     /locus_tag="VYR08_000170"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV3465760.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20440..21690)
                     /locus_tag="VYR08_000171"
     CDS             complement(20440..21690)
                     /locus_tag="VYR08_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV3465761.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21757..22584)
                     /gene="purR"
                     /locus_tag="VYR08_000172"
     CDS             complement(21757..22584)
                     /gene="purR"
                     /locus_tag="VYR08_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV3465762.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22851..23792)
                     /locus_tag="VYR08_000173"
     CDS             complement(22851..23792)
                     /locus_tag="VYR08_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV3465763.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(23782..25038)
                     /locus_tag="VYR08_000174"
     CDS             complement(23782..25038)
                     /locus_tag="VYR08_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV3465764.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            complement(25040..25702)
                     /locus_tag="VYR08_000175"
     CDS             complement(25040..25702)
                     /locus_tag="VYR08_000175"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV3465765.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKGAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25665..26321)
                     /locus_tag="VYR08_000176"
     CDS             complement(25665..26321)
                     /locus_tag="VYR08_000176"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV3465766.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(26332..27210)
                     /gene="rsgA"
                     /locus_tag="VYR08_000177"
     CDS             complement(26332..27210)
                     /gene="rsgA"
                     /locus_tag="VYR08_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV3465767.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKKELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(27212..28084)
                     /gene="rsmA"
                     /locus_tag="VYR08_000178"
     CDS             complement(27212..28084)
                     /gene="rsmA"
                     /locus_tag="VYR08_000178"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV3465768.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMTFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28470..28628)
                     /locus_tag="VYR08_000179"
     CDS             complement(28470..28628)
                     /locus_tag="VYR08_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465769.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQILVHGYL
                     LDFASIEG"
     gene            complement(28621..29262)
                     /locus_tag="VYR08_000180"
     CDS             complement(28621..29262)
                     /locus_tag="VYR08_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465770.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(29264..31294)
                     /locus_tag="VYR08_000181"
     CDS             complement(29264..31294)
                     /locus_tag="VYR08_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3465771.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31563..32462)
                     /locus_tag="VYR08_000182"
     CDS             complement(31563..32462)
                     /locus_tag="VYR08_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3465772.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(32534..33094)
                     /gene="rnmV"
                     /locus_tag="VYR08_000183"
     CDS             complement(32534..33094)
                     /gene="rnmV"
                     /locus_tag="VYR08_000183"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV3465773.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(33094..33867)
                     /locus_tag="VYR08_000184"
     CDS             complement(33094..33867)
                     /locus_tag="VYR08_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV3465774.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(34172..35053)
                     /locus_tag="VYR08_000185"
     CDS             complement(34172..35053)
                     /locus_tag="VYR08_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV3465775.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLV
                     VATSGFALLGRKTRRRK"
     gene            complement(35337..35471)
                     /gene="rpmH"
                     /locus_tag="VYR08_000186"
     CDS             complement(35337..35471)
                     /gene="rpmH"
                     /locus_tag="VYR08_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV3465776.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(35616..36830)
                     /locus_tag="VYR08_000187"
     CDS             complement(35616..36830)
                     /locus_tag="VYR08_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3465777.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(36986..37279)
                     /locus_tag="VYR08_000188"
     CDS             complement(36986..37279)
                     /locus_tag="VYR08_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465778.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
BASE COUNT        10522 a         8689 c         7178 g        10902 t
ORIGIN      
        1 gagattttcc tcctacaacc gtttcaaaag aaaactatct ttattccatg ttattttgag
       61 gaagatattg tgaaagtcaa agatgatgat aaagttgagt ggaatttgtt agaatttcaa
      121 aaatttagag catttttggc ttagtaatct gtgttgaagg ctcaaaacct atggtaaaaa
      181 agtcgctttg aaaacgtatt gcctccaaag atttagttaa ataatgattt aacacaaaaa
      241 gaaattattg aagttctgga aagatgttgt ttcagtattg agaaaaggtg ggaaaaactt
      301 gcgattttca cagagaaagg aagaaaaagt atagaaatat agtcaattga aacaagaaca
      361 ggacaaaaga accttttgtg ccatattttt ctcctttcgc tttacaattg gcttgaacac
      421 ctttattgta tcgcgtttgg agtttttttg gtataacctt cgacgcgcac ccgcatagcg
      481 ggtgtttttt tgtctcgcac ctaacggagc gagacgaact aatagtcact taatcaaaaa
      541 acgcaccata tcaaaaacta aaaagtttga tatcatgcgt catgtcttaa actaattgac
      601 tatactttct attcaaatga gcttttaacc aattgattga gccaatccac tcttaaaacc
      661 aaagagcaat ttctcgctta gctgactctt ctgaatctga accatgtaca acattttgga
      721 taacctcatt ttctccagca gcttttgcaa agtcacctcg aatagtgcct ggtaaagctt
      781 cttctggacg agttgcaccc atcatggtcc gccaagtttc gattactttg ggaccagaaa
      841 tgacacccac aagaactgga cctgaagtca tgaattcacg aatcggtagg taaaaactct
      901 gaccaaccaa gtcctgatag tgctggtcaa tcaactcttc tgaaacctgt gaacgaaact
      961 ccaatttttc gattgtaaat ccacgttgtt cgatgcgctt taacacttca cccactagcc
     1021 ctctttttac accatctggt ttgatgataa agaatgtttg ttccataccc gtctcctttg
     1081 tcagcttctt tcttttattt taccacattt cgtggaaaaa tggagaaagt tttcagaaga
     1141 gagaatgaga gaacccgagg gttctctcat tctctcttat tctactgttt cttccacagt
     1201 ttcaacggca gtatccacaa ctacttctgt tgtttcttca tttccttctt cctctactgg
     1261 aggattaagg tattcttctt cgttgacagc atgtggttca aggttacggt aacgggccat
     1321 accagtacca gctgggatga tcttaccgat gataacattt tctttaagtc caaggagatg
     1381 gtctttctta ccacggatag ctgcgtcagt aaggacacga gttgtttctt ggaaggaagc
     1441 tgctgacaag aaactgtttg tttcaagtga ggctttggta attcccataa ggactgggcg
     1501 acctgtcgct ggaactccac ctgcgataag gacatctttg ttggcatctg taaagtcatt
     1561 gatatccatg agggtaccca tgagaagatc tgtatcacct ggatccatga cacggacttt
     1621 acggatcatt tgacgaacca ttacctcgat gtgtttgtca ccgatttcta ccccttggct
     1681 acggtaaact ttttgtactt caccgagaag gtacgtttca actgacaaga catcacgaac
     1741 tgcaaggaga cgttttggtt ggatagaacc ttctgtcaga gcagcaccac gcgctacttg
     1801 gcccccaact tcgacacgca tacgagctgt aaatggaacg acatattcac cttcgccagt
     1861 ttcaccctta acaaagactt tcttggtacg agttgatgca tcttcttcga tagcagtaac
     1921 ttgtccttta acctctgtga taaccgcttc ccctttagga ttacgggctt caaagatttc
     1981 ttggacacga ggaagaccct gagtgatatc ggtatttgag gcaaccccac ctgtgtggaa
     2041 ggtacgcatt gtaagctgtg taccaggttc cccgatagat tgggcagcaa ttgtaccaac
     2101 tgcttcacca acttcaaccg catcaccagt cgccaagttg ataccgtaac agtgacggca
     2161 gacaccgtga cgagtgttac atgtaaatac agaacggata gtcacttctt ccacaccagc
     2221 attgacaatt tcacgcgcct tgtcttctgt aatcaattca tttggaccaa taatcactgc
     2281 accagtttct ggatgtttaa cagttttctt agtgtaacga ccgttgagac gctcttcgag
     2341 agactcgatc atctcttttc cttctgcgat agaacggatc aagagaccac ggtcagttcc
     2401 acagtcgtcc tcacggatga taacgtcttg ggcaacgtcg accaaacgac gagtcaagta
     2461 acctgagtcg gctgtcttaa gggccgtatc ggtcatacct ttacgagcac cgtgagttga
     2521 gaagaacatt tccaataccg acaaaccttc gcggaagttt gaaaggattg gcaattccat
     2581 gatacgtcca ttcggagcag ccatcagacc acgcataccg gcaagctgtg agaagtttga
     2641 gatgttacca cgggctccag agtccatcat cataacgatt gggttcttag gatcttggtt
     2701 agcaatcaag cgtttctcaa gtttttcacg ggcagcacgc cattcagctg taacagcatt
     2761 gtaacgctcg tcgtctgtga tcataccacg acggaattgt ttggtgattt gttcgacacg
     2821 tttgtgtgat tcttcaatga tttcagcctt gtcatcaacg actgggatat cggcaatacc
     2881 cactgtcaat cctgcaagag ttgagtggtg gtaaccgagg ttcttcatgc ggtcaagtag
     2941 ggcagaagtt tctgtcgtac ggaaacgttt gaagatttca gcgatgatat ttccaaggtt
     3001 tttcttcttg aatggagggt tgagctcaag attgctgata gcttccttga tatctccacc
     3061 aagtggcaag aagtatttag ctggaacacc ttctgtcaag ttggcattgt ttggttcttg
     3121 caagtatggt agcccctctg gcatgatatc gttgaagaga attttaccaa ctgttgtaag
     3181 caagacctta tgtctttgct cttctgtcca aggcttgttg aggctgtctg ttgcgatacc
     3241 aacacgtgag tggaggtgaa cataaccatt gcggtaagcc ataaccgctt cgtcacggtc
     3301 tttgaagacc attccttcac cttcgcgacc agcttcttcc atggtcaagt agtagttacc
     3361 caaaaccatg tcctgagatg gagtaactac cggtttccca tctttcgggt tcaagatgtg
     3421 ctcagcagct agcatgagga tacgagcttc tgcttgtgct tcttctgaaa gtggtacgtg
     3481 gatggccatt tggtccccgt caaagtcagc attgtaggct tcacagacaa gtgggtgcaa
     3541 gcgaagagcc ttaccatcaa tcaagactgg ctcgaaggct tggataccca aacggtgaag
     3601 ggtcggtgcg cggttcaaaa gcactgggtg ttctttaatc acttcttcaa ggatatccca
     3661 gatacgctca tctccgcgtt ccaccaagcg tttagctgct ttgacgtttt gcacgatatc
     3721 acgggcaacg atttcacgca tgacaaatgg tttaaagagt tcaatcgcca tttcacgcgg
     3781 cacaccacat tggtacatct taagagttgg accaacggcg ataactgaac gtcctgagaa
     3841 gtcaacacgt ttaccgagca agttttgacg gaagcgtcct tgtttacctt taagcatgtg
     3901 gctcaatgat ttcaatggac ggctacctgg tcctgtgatt ggacgaccac gacgaccatt
     3961 gtcaatcaaa gcgtcaactg cttcttgaag catacgcttc tcattttgaa cgatgatacc
     4021 tggtgcattt aactcaagca aacgagccaa acggttgtta cggttgataa cacggcggta
     4081 aaggtcattc aagtcagatg aggcaaaacg gccaccatcc aactgcaaca ttggacgaag
     4141 atctggtggg ataaccggaa ggatgttaag aatcatccat tcaggtttgt ttccagactt
     4201 gtaaaaggca tccaaaacat ccaaacgacg gatggctttg acacgttttt gtccagtagc
     4261 agttttcaat tcttctttga gttcagcaat ttctttttca agatctactt gcttcaaaag
     4321 gtcttggatg gcttccgcac ccatcttagc aacaaatgaa ccataaccat attcacgcaa
     4381 gcgctctcgg tattcgcgct ctgtcatgat agacttgtgc tcaagtggtg tatccttagg
     4441 atcaatcacc acataagccg caaagtagat aacttcctcg agggcacgag ggctcatatc
     4501 aagggtcaag cccatacggc ttggaatccc cttgaagtac cagatgtgag atacaggagc
     4561 tttcaattcg atatgtccca tacgctcacg acgaactttc gtacgcgtta cttcaacccc
     4621 acagcggtca caaacaattc ctctgtaacg aatgcgtttg tacttaccac aagcacattc
     4681 ccagtctttt gtaggaccaa agatcacttc atcaaagagt ccttcacgtt ctggtttcaa
     4741 ggtacggtaa ttgattgttt caggtttttt gacttctcca taagaccatg aacggacttt
     4801 acttggagaa gctagggtga tttgcatact tttaaaacga tttacatcaa ccactatttc
     4861 ttccctttct attctaagtg aactgcttat tcttgttcag cagcttcttc tgttgcttcc
     4921 gcttttgttg ctttctcagc ttcttcagct tcaaaggctg ctttagcctc ttgggctgct
     4981 ttttcgcggg ctttttcaag gtcatctacg tggatgacat cttcgtccat tccttcatcc
     5041 aagtcgcgaa gttccacttc ttggtcatct tcgtctagga cacgcatgtc aagaccaaga
     5101 gattgcaatt ctttgacaag aactcggaag gattctggaa cacctggttt tggaattggt
     5161 ttgccttttg taatagcttc ataggctttc aaacgtccgt tgatatcgtc agacttgtaa
     5221 gtcaagattt cttgaaggac atttgacgca ccgtaggctt caagagccca aacctccatc
     5281 tcaccgaaac gttgtccacc aaactgagct ttacctccga gtggttgttg ggtaacagtt
     5341 gaataaggtc cgactgaacg cgcgtgcaat ttatcgtcaa ccatgtggtg gagtttgatc
     5401 atgtacatga ctccaacaga aacacggtta tcaaatggtt cacctgtacg tccatcgtaa
     5461 aggattgtct tggcatcgct atccatacct gcttctttaa cagttgacca aagatcttca
     5521 gaacttgctc catcaaagac tggtgtcgca atgtgaatac caagagtacg agctgccata
     5581 ccaaggtgaa gctccataac ctgaccgata ttcatacgtg atggcacccc aagtgggttc
     5641 aacatgatgt cgactggagt tccgtctgga aggtaaggca tgtcttctac aggaacgata
     5701 cgagagacaa cccctttgtt tccgtgacgt ccggccattt tatctccgac cttaatctta
     5761 cgtttttgag cgatgtaaac acgaaccaac atgttaacac ctgattgcaa ctcatctcca
     5821 tttacacgtg taaagatctt aacatcacga acgacaccat cggcaccgtg tggtacacga
     5881 agagaagtat cacgcacttc acgagacttg tctccaaaga tagcgtgcaa gagacgttct
     5941 tcagctgaaa gatctttctc acccttaggt gttactttac ctacaagaat atcaccttct
     6001 ttaacctcag caccaatacg gataatcccc atttcgtcaa ggtctttgag ggcatcttca
     6061 ccaacgtttg gaatttcgcg agtgatttct tcaggcccaa gctttgtatc gcgcgtttct
     6121 gattcgtatt cttcaaggtg aacagatgtg tagacatcgt ccttcaccaa gcgttcgctc
     6181 atgataacgg catcctcgaa gttgtaacct tcccaagtca tgtaggcaac gattgggttt
     6241 tgtccaagcg ccatttctcc attttccata gaaggtccgt cagcgatgaa atcgcctttt
     6301 tcaacgacat caccaacttt tacgagagtg cgttggttgt aagcagtacc tgagtttgaa
     6361 cgacggaatt tttggatgtg gtaaacatcc aatgaaccat cttcacgacg aacttctacc
     6421 ttgtcagcat ctgcgtaagt aactttacca tcatactgag caatcacagc cgcaccagaa
     6481 tcgtgggctg cttggtattc cataccagta ccaacgtaag gtgcctgagg attaatcaat
     6541 ggcacagcct gacgttgcat attggctccc atgagggcac ggttggagtc atcgttttcc
     6601 aagaaaggaa tacatgctgt cgcaacggca actacctgtt ttggtgaaac gtccatgtag
     6661 tcaacaatat tagctggata ctcttggttg accccttggt gacgtcccat gacaatcttc
     6721 tcagcaaagg ttccatcttc attcagacga gagttagcct gagctacagt atattcatct
     6781 tcttcatcag ctgtcaacca aacaatttcg ttcgtgacaa cacctgtttc acggtcaacc
     6841 ttacggtatg gtgtttgaac aaaaccatat ttgttcaagt gtccgtaaga tgacaagtta
     6901 ttgatcaaac cgatgttagg tccttcaggt gtctcgattg gacacatacg accatagtga
     6961 gtgtagtgca cgtcacgtac ttcatatccg gcacggtcac gagtcaaacc accaggtcct
     7021 aaggctgaca aacggcgttt gtgagacaac tcagaaagcg ggttgtgttg gtccatgaac
     7081 tgtgacaact gtgatgaacc aaagaattct ttaactgcag ctgttacagg acggatattg
     7141 ataatttgtt gtggtgtcaa gacttcattg tcctgaacag acatacgttc acggacatta
     7201 cgttccatac gagaaagtcc caaacgtact tggttggcaa gcaattcacc aaccgcacgg
     7261 atacgacgat ttccaaggtg gtcgatatca tctacacggc caagtccttc agccaagttg
     7321 aggaagtagc tcatctcagc aaggatatct gcaggagtca ccgtacgaac cttgtcatct
     7381 gggttagcat taccaatgat cgttacgacg cgatctggat cagttggagc aacaaccttg
     7441 aatttttgaa gaacaacagg ctcagtcaca acggctgcat cgtttgggat gtagacaatc
     7501 ttgttcaagt cgccatccaa atggctttca atgctttcaa tcacgctacg agtcataatc
     7561 gtaccagctt ctaccaagat ttctccagtt tcagggtcta ccaatggctc tgcaatggtt
     7621 tggttgagca aacgtgtttt aacattgagt tttttattga ttttgtaacg accaactgct
     7681 gccaagtcat aacgacgtgg gtcaaagaag cgagctacaa gcaagctacg tgagctttca
     7741 gccgtcttag gctcacctgg acgaaggcgt tcgtaaattt ctttcaaggc ttcgtctgta
     7801 cgagagtcca ttggattctt gtggatatct ttttcaacag tgttgcgaac caattcgctg
     7861 tcaccaaaga tatcaaagat ttcatcatca cctgagaaac caagagcacg aaccaaggtt
     7921 gtaaatggaa tcttacgagt acggtcgata cgagtgtagg tgatatcttt tgagtcgctt
     7981 tcaagttcca accaagctcc acggttaggg ataacagttg aaccatagcc caccttacca
     8041 tttttgtcta ctttgtcgtt aaagtaaaca cctggtgagc ggaccaactg agaaacgata
     8101 atacgttcac caccattgat gatgaaagta cccatttctg tcatgattgg gaaatcacca
     8161 aagaaaactt cttgggtctt gatttcgctt gtttctttat tgatcaaacg gaaggttaca
     8221 aaaattggtg ctgagtagct agcatcgtgg atacgagctt cttctagcgt atattttggt
     8281 tccttgattt catatccaac aaattccaac tccattgtgt ctgtgaagtt tgaaattggc
     8341 aatacatctt caaacacttc cttaagaccg tggtctagga aagctttgaa tgagtcagtt
     8401 tgaatttcaa tcaaatttgg taagtcaaga acttctttga ttcttgaaaa actacgacgg
     8461 gtacgatgtt tcccgtattg aacgtcatgt cctgccaaga tgattctcct ttgtaaataa
     8521 gttccaagcc ttgtcaatca ggcttttcta atcgtcatat ggttgtaaac cccttatcac
     8581 cgtgtcctct tgacgaattt tcagaatctt taagcctctg ttacaaatgc tcaaaatctt
     8641 gaaaaaaagc acaaaaagag cagctaaatc tgactttttc agaagattta actgctgtga
     8701 gccttgtctg gacaatattt cagacaaaac ctacgacaaa tgattaccca tattataccc
     8761 tatttagcta gatttttcaa ggggtttcag taggtttttg gtaatttttt tcccatagaa
     8821 aacttggcat cacattcgaa tcacgctatg gtacaaaaaa ctgaaaaaac tattgactga
     8881 aaatcatttt caaggtataa taataaacgt taaggcggta tagccaagtg gtaaggcacg
     8941 gctctgcaaa agcttgatcg tcggttcaaa tccgtctacc gccttctata acttgattta
     9001 tcaggtttca aatgaacaga aagcccaatt tgaagggctt tttttatttt ccctcgaata
     9061 aatacgtata actttaaaaa cttttggagc gagtttgtgg cagagttctt tccatggcat
     9121 aattcccttt tgaaatcaat tgaaaaaagc ttcgtcacgc ctggtgacaa aagcctctag
     9181 tttattctgt ttcttcttct atttcgactt ctgtgatttg gacttttacc ttggtaacac
     9241 gtccattctt cactttatca ttggttaaga taaactgttt gttttggctg accaattcat
     9301 agctgagttt ctcagtcgtt ggaatggttc ccactcctgt caaataataa ccagcgatgg
     9361 tatcaacgtc atcactttcc agttcaacat caaagtaatt attgaaatca ttgagggtca
     9421 tagttccttg aacaatgtag gtatcctcgc caatctgatg aacatctatt gctgctttgt
     9481 ccgtttcatc atcaatttca ccaacaattt cctctaagag gtcttccaaa gtcaccaaac
     9541 cagccatacc accgtattca tccagcaaga ttgccatttg tctttgggta tttcgcagtt
     9601 cttttagcaa gtcatccaca aaaatagttt caggtacaaa aagtggatct tgtaaaattc
     9661 tcttccaaac aatattgtca aaaccgtcca caaagcctgc cttaaggaga ctcttggtgt
     9721 gaatgattcc aattacattg tccttatccc catcataaac cgggatacga gaataatttt
     9781 gttttaaaat actttggata atggcttgac tatcatcctg aatatccacc ataaaggcat
     9841 ccgttcgagg aaccataacc tctcgtgcca tcagttcatc gagcgaaaag acaccttgta
     9901 gcatctcaat ctcatcagca tccaatgttt cttcactatt tgtcagcata taggcaattt
     9961 catcacgggt catcttttca tccgcatcat cgaatgacat aggagtcaaa tggctcaaga
    10021 aattggtcga agcagctaaa agccaaacaa aaggactgac tagttttccg atcccaatga
    10081 taatcggcgc tgtacgaatt gccaaggcat cctttagatt aagagcgatt ctcttaggat
    10141 ataattcccc aaaaacgatg gaaatatagg tcaaaaatgc caaggataga aaagttgcca
    10201 cggcttgtgc tgtttcgcca ttcccaagcc aagaggcaat cacacgtcct agagtatcag
    10261 ttaaactcgc ccctgataag attgtaatca gggtgattcc tacctgaatg gttgataaaa
    10321 agtggttagg attttctagt accttcagca ggcggatgta gcgtctgtct ccttcttccg
    10381 ccttttgttc aactcgggca cgattaagag aaaccatggc catttctgtg gctgagaaaa
    10441 aggcatttaa taccgtcaag ataaacaata aaacaaattg tagcaacaaa ttctgactac
    10501 ttgggtcttc catagttctt ctcctaaaat agtatcttat cctttattat atcacaaaat
    10561 ttaatccaat acatattatt tttttcttag cgtaacttcc atctctctcc caaaactgga
    10621 agttagtcta agacggattt ccactgattt cacgcgtttt tctcatacta aaagctaggt
    10681 tgtgggaaac tggaagttag ttttagaagt tactattttt tcttagtacc tgttctagcc
    10741 ctgcttaaac tagtaaaaag aactcctaca tccataagtg acataggagt ttaggcttat
    10801 cttttactga gttacagtta cttctccagt tcggtaatcc agttgaagtc ccttttgaac
    10861 attgggaact agaacattga attccaattc tccatccgaa gacttggctt caatctgtga
    10921 agctgaggga actaaatcct cgtttgaagc gtagtaaagg gttacacggt aacggacacg
    10981 cttgttttgg tccaaggctt tacgcacctt gctttcatag tagttttgac cagtcgaata
    11041 ctcggcttgt gcctgatttg cccaggctgt ctgaacagca atgtttttag gattgcttgt
    11101 tgaggcatca aaaccatcca aaccaccgat taaggcatag cctaacaaat gacctctatc
    11161 gactgcatgg gtataagagc cctttagatt cttgacctga tgccaacctg gaggagtcca
    11221 agaagttgaa ccattcccag tttctttacg attcttgtac tgacgagtgg cattagacaa
    11281 gagggcatta gctacggttg gaacagtttc cttgcccact gtctttgttt tattgtcagc
    11341 gtagggctta cttgaaacct tggcatctag atttgtttta ttaccattga cgataaaagc
    11401 acctgagcca ttccactcca gactcccctt tatttgactc ttgactgcgt ctgttaagac
    11461 actctctgcc aatgtttgac taggagcttc agacgcttgt tttttctgac taagattggt
    11521 tttgggacta ttaggtgccg actgcatctg cttgatataa tagctccctg tagacaaaag
    11581 caataacact agcagtccga ttagtgtctg tcttgttttt ttgttcatat ttctccttat
    11641 ctttagaaaa aggctggttc tccagcctaa tttcctgtaa atttatgaat caattcctgc
    11701 cattttgctg gagacaggat agcccatgga tcttgaccct tgcccaagat tccataacct
    11761 accattagac cgattcctag agccagagta cctaaaatca gtactatgat gactaaaagt
    11821 aaacgattga ttacgtagct tgatttctta ttcatcttca tcgcttgcct caatccgctg
    11881 aatcttagca cctagctgcg ccaacttctc atggaaaccg tagtaacctc tatccaagtg
    11941 aaccaattta ccgaccacag tttctccctg tgctaccaaa cctgtcaaaa tcaaggctgc
    12001 actggcacga aggtcagttg aaagaacttc tgctccctgc aaaggctgtc caccaacaat
    12061 acgagctgta tcacggataa tctcagaatg caagcccatg cggcgcatct cttctaggtg
    12121 ttggaaacga ttttcaaaaa ctgtctccac catggttgat tcgccttttg caactgtcat
    12181 caaggctgta aattgagcct gcatatctgt tggaaatcct gggtggggca aggttttcac
    12241 atgaacagct tttagatttt ctagttgaga acgaacacga attccttcgt cttcttcaat
    12301 tacttcaaca cccatttcaa gtaacttggc aatcaaggga cggttgtgct cccagacagc
    12361 gtctcgaatc aagacatcac caccagtcat ggcagcagct accataaagg ttcctgcttc
    12421 gatacggtct tggactacat tgtgagtcgt gccatgaagt ttctcaacac cagtaatggt
    12481 tatagtctct gtaccagcac ctttgacctt ggctcccatt tcattaagga gaatggctaa
    12541 gtcaacaatc tcaggctcac gcgcagcatt ctcaatcact gtcaccccat cagccagagt
    12601 cgctgccatc atcaagttct gcgttgcacc aacacttgga aagtccatat agatatgagc
    12661 gccatgcaag cgttctgcct tggcttcgat gtaaccagct gtctgactaa tcttaacccc
    12721 catagcttcc agacctttca aatgaagatc aataggacgg ataccaatcg tacaaccacc
    12781 tggcatggat accttggcat gacccacacg ggcaaggatt ggccctaata caacgatgga
    12841 ggcgcgcatc ttgctgacat acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc
    12901 caccttgaca agatgagctt cctcatcaaa gtcaaccttg gcattcaaac caccaactac
    12961 ctgattcata ataaagacat ccgacaaaat cggaacattc tgcaagacgg tctttccttc
    13021 acttgctaga atagtcgctg ccaacaaggg taagactgca ttttttgctc cctcgatcgt
    13081 cacgcttcct accagacgat tatcgccacc ttgaaccaca attttatcca tactcgtttc
    13141 ctttactctt gatttcataa taatcctcta agcgcttctt gccacaaatg atacaggcta
    13201 ataaagaaag aactcaggat gtagcctatg acaatgctaa aaaaggctac taataaacga
    13261 acttttcttg tattatcatc tgtcactttt aaaaccttct cccatctaac aagatccttt
    13321 aaaaggtaaa aactcacata aataaagaac atatgactac ttagagtgaa taataattga
    13381 accatttgac tattatacca tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa
    13441 aattagggat tgtttttcaa gtaatcaatc gcatcttgta gggttttaac gggcacgatt
    13501 ttcatatccg tcttgattgt tttagccgct tctagggctg tttggtagtt gtttttcgcg
    13561 tccggatgcg ccttttgttc ttcttcgcta acagggttat caggagcaaa gaaaatagca
    13621 gcacctgccc tagccgaagc tacaaccttc ttatcaatac ctccaatgtc tcccacatta
    13681 ccatcgcggt caatggtacc tgtaccggca acaatacgac cattacgaag atctgggtga
    13741 gctatttgag tatagatagc tagactaaac atgagaccag cacttggacc gccaatacca
    13801 gctgttgaaa agctaattgg gacattgctg attacctctg tacggtcaat caagccgatt
    13861 ccaattccat ttttgccatt ttccaaggtg ataatctttc cttctgcaga cttggtttgc
    13921 ccatcctctt cataggtgac cttgacggag tcccctaatt tttgagaact gacgtaatca
    13981 atcaagtctt tggaactatc aaaggtctga tcattgactg ctgtgactgt atcagagata
    14041 ttgagaatcc ctttaaaggt tgaattatcc gtcacattca aaacataaac tccaaagtac
    14101 ttgagttcga tatccttacc agctgttttt agtccttgat acttggccat attttgcgat
    14161 gtttgcatgt agaattgatt gattcgcata aattcaacat cggaagaacc acctgtagtc
    14221 tcctgagcac tacgaatatc tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa
    14281 gtagcatgtt gaacaccaac cgtaacgaat tgataggcac cagcttcctt atcttctgtg
    14341 tcatttactt taaggacttg gcgaatatct tccgaaccac ctggaacctc tatatagtag
    14401 ggcaaaggca ccacaaatgc caagaaggtc acaatcaagg ccgcaatgac atacaaaggc
    14461 catctaatct tttttttcat ttcttatttc ctccaaaata ctctcgggaa catggcaggc
    14521 aatatcctga ccaaacttca aaagctctcg aacacctgat gaactgatat agagatgttc
    14581 aggccggcta tgtaaataaa tagtctctat atcagaagac agctgatgat tgtagtaatc
    14641 aaaactagct tcatattgca aatccgccgc attcctcaac ccccgtacta ggaaagtagc
    14701 ccccagtctt tttgcaacat cgaccaccaa tttatcatga gaagccacga cttcaacatt
    14761 ttccaaatgt cccaaagcct tttctagccc ccgtttacga ttttcaagag ggagaaatcc
    14821 ttgtttgtgg ggattaaaaa aaatacccac ataaagctta tcaaagagtc tgctcgcccg
    14881 ttcaatgata tccagatgcc catttgtcat cggatcaaat gagcctgtga ataagccaat
    14941 cttatctgac atagactgtc accttactaa ttccataaat cttttccttc cagataccca
    15001 gacaggcaat ttcttctgga agttcaacgg ctttatccgt ctcgcacaca accataacat
    15061 cttcagaaaa aagctctctc tcagccattt tttcaatatc tgctacgatt tgttccttgg
    15121 catagggagg gtctaagaaa acgaggtcaa attccccaga tacctgttcc aatgccctct
    15181 ctgcatccat cttgaggagt tgaaattttc caacttcctt ggtcatctgg atattttcag
    15241 ccacgatggt ctgagcctta cggtctcgct ccaccaaaac agcactggac atgccacgcg
    15301 atactgcttc gatagataaa ccaccactac ctgcataaag gtccaagact cgtccccctt
    15361 caaagtaggg accaatcatg ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg
    15421 ttgtcttgcc ttctagtgtc ttgaggggac gtcccccata gattcctgat acgattttca
    15481 tactgtttat tataccaaat tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg
    15541 attctctaca aaatattttc gtaagtatcg cggacttctt gaggccaaac acttgtttgc
    15601 acttctccaa tgtgtctctt gcgaagtagg aacatggcca tacgagattg tccaattcct
    15661 ccaccgattg tcaatgggaa taggccattc aacaaagact tgtgccattc caattccaag
    15721 cggtcttcat cacctgtaat ttccacctga cgtctaagag tttcttcatc tacacgaatt
    15781 cccatagaag acaactcaaa ggctccacct aaagactcat tccagacaag aatatcacca
    15841 tttagaccct tgtagccatt ctcagactcg cttgtccagt catcatagtc tggtgcacgt
    15901 ccatcgtgcg gtttaccatc tggcaactcg ccaccgatac caatcaaaaa gacggctcca
    15961 aattctttac aaatcgcatt ttcacgttct ttcggtgtca agtctgggta gcgttctacc
    16021 aattcttctg tatggataaa ggtgatttgt tttggcaaga tagactcgat gtcatagcgg
    16081 gcttcaacag ctagctcagt caggcgaata gccttataaa tcttctcaac tgtttctttt
    16141 agataaacga tgttacgctt accatttggg ataaccttct cccagtccca ctggtcaaca
    16201 taaacagagt gggttgcatc caaggaatcc tcatctggac gaagggcttt catgtggaca
    16261 aagagaccct ctccttcacc aaagccaaaa cgagccaagg tgtggcgttt ccatttagca
    16321 agtgagtgca ccacttcata agtagcatca gggatttgga gaaccttgac cgatactgga
    16381 ttttccacac cagacaggtt gtcctgcatt ccgtcaccga ccttactcaa gataggacct
    16441 tgaacttcga caacttctag cttatctttc aaatactggg taaaagtgtt tttgacaaag
    16501 gaaatttctt cttgttgatg gataaaactt ttcttcatag acaactcctc aaaaatttaa
    16561 taaacgtatt atacacctat ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg
    16621 tcactcaaaa cactgatttc atagaccttt tagtcattgc gaccaaagat acgtagaata
    16681 cttaggaaaa gattgataaa atcaagatag atgctgagag ccattgacac cacccagcct
    16741 gtcgctacac gaccttgtga ttgttcatag gcgaggcgaa tcttttggtt atcccaagca
    16801 atcaaccctg aaaaaaccaa aaccatggct acgctaatca tataatcaaa gaagccacta
    16861 gccaagaaaa tattgactac catagcaatc agtaaaccaa tgagagctgc catcattgct
    16921 cgaccaatcc cactcaagtc tttcttggta aaaataccaa ctgccgccat gacaaagaag
    16981 agaagggcgc tcgatacaaa ggcagataaa actgtacccg gagtatagaa agccaccaca
    17041 aaactaaggg taaagccgtt taaaacggag taaagtaaaa atactggaag agcagctgga
    17101 ctatttcttg aagccatgct actggcaacg aaaaccaaag ccaactccgc aaaagtagca
    17161 atggtcaacc agagacgccc ctgcatcaaa aagtaaacca actgagactg aaagaccgtc
    17221 aacataaggc ctgataccaa agcagatagt ccgattccca gaccaacaaa ggcataaacc
    17281 ttagcgtaaa attgattgag acctgcacgg tcatgtataa tagtgtgatt catccatttc
    17341 tccttttctg tgaacatctt tccttgatta taacaaaaaa agttaaaatt agcttaaatg
    17401 gaagattaaa ataccggctg caaccgcaac attcaaactc tcggcctgcc ccttcatgct
    17461 aatatggacc aactggtctg cactttcagc cataagggga ctaattcctt gaccctcatt
    17521 tcccataact agtacaaaat tttctataga aggcagttct ctgtaatcaa cagaatcttt
    17581 agatagggtt gttgctagca ctgggatagc taccttttta gtttcgtcaa gaagcgcttg
    17641 actagtcatc cggtaaatgg gcagatggaa atgactacct tgcatggaac gtagggtttt
    17701 gagactgtag atgtctgccg acttatctga aacaatcact cctgtaaaac ctgctgcatc
    17761 cgcagttcga atgatagttc ctacattacc aggatcttgc acatcttcca aaaacaagaa
    17821 cttgccctga ctcaaatcag cttgtcctac ttcttctttt tgaaccacgg caacaattcc
    17881 ctgtggagtc tgagaatccg ccaaatctag caaaatatcc tctgaaatcc agacagtttg
    17941 cggaaaagca gctaactgat ttcgataatt ttctagggca aagattttct caatcgtcac
    18001 tccagcttgg acagcttctt caaacaagtg ccagccttca atcaaatagg cagacttgcg
    18061 gtattttttt tggtgtaatt tcttggcatt ttttaccaca gaattggctt ttgaggttat
    18121 aatagtcata gaaatattat aacacaatca aaggggtttg gtatgcaaaa ggttagaatg
    18181 attgcccaag gtagggtaca gggagtcggc tttcgttggg gtgtttacag cttggcactt
    18241 gaaattggtg gcatcacagg tcgagtatgg aataacgacg atggcacagt ggaaatctta
    18301 gcccaagcag actcatctgc tatcatggca aaatttatcc aagaaatccg aaaaggaccg
    18361 acaccttttt caaaagtaag ctacttagat gtcaaactaa gcaactttcc tccctactct
    18421 gattttaaaa tcgcaaatta ggtctccaga actattgtat attttgtaaa aaaacagtag
    18481 aatagaaagg tataattttt aaagaaggaa taaaaacagt gaaatctatt aaacgttttg
    18541 cattctcggc tatgggagtg gctatgttgc ttgtcttgac tggctgtgtc aatgtcgata
    18601 aaaccacagg tcagccaaca ggatttattt ggaatacgat cggagcgcct atggctgaag
    18661 ccatcaagta cttcgctact gataaaggtc taggctttgg tgtcgctatc attatcgtaa
    18721 ccattatcgt acgcttgatt atcttaccac ttggtatcta ccaatcatgg aaggcaacgc
    18781 ttcactctga aaagatgaac gctctcaagc atgtccttga gccacaccaa acacgtctca
    18841 aagaagcaac tactcaagaa gaaaaactcg aagcccaaca agccctcttt gccgctcaaa
    18901 aagagcacgg catcagcatg tttggtggtg taggatgttt ccctatcctc cttcaaatgc
    18961 ctttcttctc tgctatctac tttgctgccc aacatactga aggggttgct caagcaagct
    19021 acctaggcat tcctctaggt tctccaagta tgattttggt tgcctgtgct ggtgtccttt
    19081 actatcttca atcgctcctt tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa
    19141 tcaagaaaat gatttacatg agcccactca tgatcgtcgt cttctccctc ttctcaccag
    19201 ctagtgtcac actttactgg gttgtcggtg gtttcatgat gattctccaa cagtttatcg
    19261 tcaactatat cgttcgtcca aaacttcgca aaaaagtccg tgaagaacta gccaagaacc
    19321 caccaaaagc aagtgctttc tctaaaccaa gtggacgaaa agacgttacc cctgaacaac
    19381 caactgctat cacaagcaag aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga
    19441 gataagaaga aaaaggctta gctgatttgc taagtctttt tgcaaatcaa aagcccgatg
    19501 tttccatcag gcttcttttc tatccatgta cacgtttcat ataatcttga taactttcgg
    19561 tatccatgag ttttttagcg ttcttgacgc gatctgctgt tggtggtttg actccttcga
    19621 gggaatatgg aattccaagt tcacgccact tgaactcacc catggtgtga taaggtagaa
    19681 tttcaaactt atcaacattt ttgagggtct tgacgaactt accaagttca atcaagtcat
    19741 catctctgtc tgtcaatcct ggaactagca cgtggcgaat ccagacaggt tttccaatat
    19801 ctgatagata ctgggcacaa gccaagatat ttttattagt ttgtctagtg acaatcttgt
    19861 gctgttcttc gttgatttcc ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt
    19921 tgtcaaactt ctcaaggtaa cgtggtttat tacggaaagg aagagcacag gtatccaagg
    19981 tacagtggat tccttgttcc ttagccttgg tgaagagagc aatcaggaaa tcaatctgca
    20041 agagagcttc tcctccactg actgtaatcc cacccttatt tccccagaaa ccacggtagc
    20101 gcaaggcctc tgtcaagaca tcatctaccg tccgttcacg tgacttattg gactccatag
    20161 cccaagtgtc tgggttgtgg caatactggc aacgcatgtg acagccctgc aaaaagacaa
    20221 taaagcgaat accaggccca tctactgacc caaagctttc tgtcgaatgc accattcctg
    20281 tcacttgtcc ataatcaatt gtttcttcag acatatcgtt accttccttg aaaacgtttt
    20341 atagttttat tatatcacga cttgaaggaa aaacaagatt tttgcctatt ttttagaaag
    20401 caatctgttt ttcaatttca ttttcaatct attatcagat tattcttcaa aatcgaagcc
    20461 atataaggtt gcaaaatagt cctcagggcg ctctgcacgg cggatttgac gggctttacc
    20521 ttcttcggta tagaggattt ccgcagaacg taatttggca ttatactggt agcccattga
    20581 aaatccgtgg gcacctgtat catgaattac cagcaaatca ccgatttctg tatgaggcag
    20641 ttcgcgattg actgcaaatt tatcattgtt ttcacagagt gaaccaacca catctaccgc
    20701 ttcagctggt ccatctggat gggtcacgtt gctaatatga tggtaagctc cgtacatagc
    20761 tggacgcatg aggttgactg ctgaggcatc cacacctaga taggtacggt aggtttcctt
    20821 cttatgagtc actcttgtga ctagagcacc gtgaggtgcc agcataaaac gacccaattc
    20881 ggtgaaaatc ttgacctgac caagacctgc tgacgtaaga acttcttcat acaccttacg
    20941 aactccctca ccaatcaagg cgatatcgtt cggctcctgg tctggacgat aattaacacc
    21001 aataccgcca gaaagattga taaagtctag ccaaatgccc aacttttcct tgatttcaac
    21061 agccagttca aagagctgac gagccaactc tggataatag agatgggtca cggtattgga
    21121 cgctaggaag gagtgaatcc caaaagtctt ggctcctttt tccttcaaga tagcaaaggc
    21181 ttcaaagagc tggtccttgg tcatgccaaa cttagcctcc ccaggattgt ccataatgtc
    21241 tgtccccagt tcaaaaacgc ctccaggatt ataacgacaa gagatgattt ctggaatacc
    21301 tgctgctctc tccagatgtt caatatcttc aaaggcatcc aagttaatgg tcgcacccaa
    21361 ttcacgtgca taggcgtatt ccttgtctgg cgtgttgttg gaagagaaca taatctcaga
    21421 acccagaaag tccagtttat ggctcatcaa aagctctaca taactagagc agtccacacc
    21481 acaaccttct tcttggagaa ttttcaaaat agctggagtt ggagtagcct taactgcaaa
    21541 atattcctta aagcccttgt tccacgaaaa agcttggttg acggctcttg ccttctcacg
    21601 aatccccttc tcatcataca agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa
    21661 ctcttctcta ttgataaatg gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa
    21721 ggctgggaca atcgttccaa cctttagttc tatctcttat ttatcttcgt caaagatatt
    21781 gccaacctca acatcgatgg cttggttctt gacatcaata ttagttacct tcaagagtga
    21841 cttgtagtca aactgctttt cacgttcttc ttgggcattg tccgcaaaga ccgctacacc
    21901 tgccagttct gagtcgaact cgcgcaagag actaatcata ccgttgaccg ttccgccacc
    21961 tttcaagaag tcatccacaa tcaagacacg gctgcctgcc ttaagactac gttttgaaag
    22021 gaacattttc tcgatacggt caccacttga acctgaaaca tagttgacgc taacagttga
    22081 accttcggta attttcaggt cacggcgcac aatgacaaaa gagacattga ggacattggc
    22141 aactgcattt gcaagtggca cacccttagt tgctacggtc ataaccgcgt caattttttg
    22201 gtccataaag cttttggcaa taatacgacc aatatttttc aagatggctg gtgtgctaag
    22261 caaatcagac agatagatat aaccacctgg caagatacgg tcactttctg acaacttggt
    22321 acgcaagtct tcaaccattt ccttagcatc ctgactcgaa atagacggtg tgaagatgac
    22381 acctccgcca gccccagtca ctgtctggat atgaccgatt tcaatttcct caaaggcgcg
    22441 tttgataatg acgatatctt ctgagatgga tgatttagca gactcatact tttcagcaaa
    22501 agtattgaga ctagttagtt tataaggatt attaatcaaa tagttggaaa tgacaaccat
    22561 ccgatcactt cttcttaatt tcatttctat ttcctattcc atttttattc aaaaaatcaa
    22621 aaagcaaact agaaagctgg tcgctggtgg ttcaaaacac tgttttgaga ttgtcgatag
    22681 aactgacaaa ccctgtaata tacctgcata tatacatacg acaaagcgat actaccctag
    22741 tttgaagaga ttttcgaaga gtattcattt ttgtctttta cttattatac catattcaca
    22801 taaaaaaacg aacattctta tcctaaaaaa tgctcatttt tcttaaatta tcaatctaaa
    22861 tctggtttat agaaggaacg attatccata gcgaagattt tattggacat ctctccttta
    22921 tccaccaaag ccagagctgt tgacatcatc atcatgcttg catccagatt gtcaatcata
    22981 tggataatct ctgcctccat aatacgtgga cggactggac ttccatattc aagcaagccg
    23041 tggtgactga ggatgacatg acgaagcaaa acgacttctt ccttggtatc atcgatgccg
    23101 agttccataa ctgtcttggt aatttcgcta tcaatgagag cgatatgtcc aagaagatta
    23161 cctcgcactg tgtactctgt ctggtctggc cccgtcaact cgataacctt agctaagtca
    23221 tgcagcataa tccccgcata gagtaagctc ttattgagct gaggataaac ttcgctaata
    23281 gcgtctgcca aacgtaccat ggtcgccgta tgataagcca agcccgtttc aaaggcatgg
    23341 tggttggtct tggcggctgg ataggagtag aattccttat catacttggt gtagagattt
    23401 cggacaatcc gttgccagac aggattttca attttgaaaa tcatttgcga catgtagtca
    23461 cgaatttcct tgacatcaac tggtgacttg accttgaaat cagctgggtc attgggttca
    23521 ccagcttgag gcaggcggag agtaatttga ttgacttgag gggtattgtt ataaacttct
    23581 cggcgtcctt tcatgtggac aaccttacct gcggtaaagg cctcaatgtt atgaggttgg
    23641 gcatcccaga gcttcccatc aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag
    23701 tttttcccag ctcgagtttg cctcaggtca gctgatttga ttaggtaaaa gccttcaaat
    23761 aactcatctt ttttcatgtg actaatcttc atattcttcc tcattttctt gaaaatagag
    23821 tagatcaagc gcaggctcac cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc
    23881 tatggtacga cggtttaata attcatcaat attgccagag gcatgttgga gatgtttttg
    23941 tgccttgacc agaataccac caaacttgcc aaactcggtc ttgacactgg caagagtctt
    24001 gctgatatgg tcggcactct tttggatatt aagggtcttg aaaccaactg atagggagtt
    24061 aagaagggct gatagggtac ttggtcctgc aacaataatc tgttcttccc gtctcaaatc
    24121 atcaaagaag accggattgc gcacaatctc tgaataaaga ccttctgtcg gaacaaacaa
    24181 gattccaaag ttggttgtcc gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa
    24241 gcgcttgacg cttgctagga gtgacttacg acagcgttca atctcatcct tgtcacctgt
    24301 ctcataggct tcttccaagc ggtaataatc tgccagtgga aacttagagt caattggtag
    24361 gtagacgtat tcttggtcgc cttgtccggg taacttgatg gcatactcca ctcgttcact
    24421 agagttttca accgttgcgt attctcgttc gtactgggca ggtgtcatga tgtcttcaat
    24481 aatttgcccc agttgcaatt ctcccagaat ccctcgcgtc ttggttccag agagaacctt
    24541 gttaagagct ccgacatcac gggcaactgt ctgcatttct ccaagaccac gattgacaga
    24601 ctccagttgt ttagaaactg tctcaaagga agcctgtaag cgtgtctgca aggtcttttc
    24661 tagtttttcc tcgaccgtct ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa
    24721 ggcttggaga cgttggtctg tcttgtctct agtttggagg agattatctg tcatttcttg
    24781 acggacttgg gtcaggcctt ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac
    24841 agccacttcc aaatcttttt ggtctaactg gctggcttgt ctggcttggt caaagcggta
    24901 atccaactgg tctgacaaat gatctgcctg atcctccaag ctcttactta agtgtttctc
    24961 ctgcctatcc tgcctttgcc aaatcagaaa aagaccagct aggttggcaa ttaataatag
    25021 aataagtaaa ctttccatcc tacctcctgt ccttgctatg cagtacgacc acataaccat
    25081 ctgggcaagt taccgacact tccctatcta tatattcgtt agaagcgtac acttttttaa
    25141 agaaaaaatt ttcctctgtc aactcatact tggctccaag aatagtcagc tggctatccc
    25201 gaactggcat aaaggctaga tagtcgtagt ctgaacgagg ttctagctga ctggtccctt
    25261 ctggacaata agtaatcaag ttttgcccat cctcaatttc tatttgatgc atatagggtg
    25321 ccaacttagg attgctaggc agaaagacat tggccaacat atggtcaata cggccaccca
    25381 aggcaccgaa aatagtaacc tgagcctgag gatttttctc aaagatggtt aagagagcca
    25441 attccaaatc ggtatcatct ttttctggtc gggcttggac aaaatgctgg gcaccttttt
    25501 gaatcacctg tcgctcttct tccgtcacag aatcaaaatc cccaactgct agagcaagaa
    25561 gcaagtcttc ttccaagacc cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat
    25621 ctgtccgata atgaccgcgg tctccgcctg caaaaacggc aaccctagtc cagttgtttt
    25681 ctgagagttt gtactcgctc attgacatct cccttaaaga cataggaacc tgctacaaaa
    25741 acggtcgcac cagcttcttt ggcttgggca atagtttggt catcaatccc accatccact
    25801 tcgatttcaa agttcaaacc tttttcctca cgaagagcaa ccaactcacg aaccttatcc
    25861 atggtttctg gcagaaaggc ttgcccacca aaacctggat taaccgtcat gactaaaact
    25921 tggtcaacta gatgaaggac gtgcttgatg gcttcaactg atgtgccagg attgataacg
    25981 actgaaggct taactccgag tgaacgaatt ttttggaggg cgccatgaat atgaggcgtt
    26041 gcttctacat ggatactgat gatgtctgca cctgcacgcg caaaatcttc cagatgatgc
    26101 tcagggtttg acaccatcaa gtggcaatcg aaaaccatct tactatgagg acgaaggctc
    26161 tcgaccacac ctgcaccaaa actgatttgc ggtacaaaat gactgtccat gatatcgata
    26221 tgggcatatt ctgccccagt tgcttctaga cgtttgattt cacgttcaaa gttggcataa
    26281 tctgctgcca gaattgacgg agcaatcttg tattgagaca taggtttctc cttattttgg
    26341 aatttttttg ctgacttttt tataggtttc tctacgattt tcaatttcac taaggaattg
    26401 caggtaattg tcaaaacgga aggttgcaat aacaccctct tcaacagctg gtttgacggc
    26461 acaagacggc tcatgggtat gggtacaagt acggaactta caatctcggc taacagtagc
    26521 aatctctgga aaggcctgat tgaggtcttc agcccttgat acttcatagt ccagagatga
    26581 aaagcctggt gtatctgcga ttttaccccc gttgagattg taaaaactaa cagctcgagt
    26641 ggtatggcgc ccgcgaccca gactgtctga aatttctccc gtttcaagat taaggtctgg
    26701 tacgagttta ttgagaagag ttgacttccc aacacctgtc tgccccataa agaccgtaac
    26761 cttgcctgtt aacaaagaca ggagttcctt tttactggtc acaaagtcat agccgatgtc
    26821 accataggtc tgccggtaaa aatccagttc tcccctatct tccaacaaat ccattttgga
    26881 aatatagaca atgggatgga tgcccttgtg ctccaaaaga accaagaaac gatccagcaa
    26941 attgctgtta aaatcaggtt ccttgacgga catgattact acagcttgat cgatattgac
    27001 aataggcgga cgaaccagac tgtttttccg ttcgtgaatt tttaggatat agccttctga
    27061 attttcctcg gcagagaaat ctacccagtc cccaacataa ggggtatggc cttttttacg
    27121 gaaattccca cgcgcgcgtg tttgataaac ctggccatca ctctccacat agtagaaacc
    27181 tgccaaggct ttaatgattt gtccctgcat cttagagtcc ttgcccttta agtgcgtctg
    27241 ctagaccggc aaattctgcc aagctgagag cttccccacg cacacttggt gacaagcctg
    27301 cctggtccaa agccttggtc agcttgtcct tgacctcttc agtcttacca aagtaacctg
    27361 tcaagttatt ccacaaggtc ttacggcgat gggtaaaact agccttggaa accttaaaga
    27421 agaagttctc atcttccact gctacagctg gctctggacg gcgcaccatt ttcaagatgg
    27481 ctgaatccac atttggcgct ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg
    27541 ctgtcatgta atactgcacg gcgatagaca agctaccgta agccttggtg ttaggctggg
    27601 ctgaaatgcg gtccgctact tctttctgca tcatgaccac aaactcacta aaaggaatgc
    27661 cactctcaat caagtgcatg agaataggcg tcgtgatgta gtaaggcaaa ttagccacta
    27721 ccttgattgg caggtcagga tttttaaaat tctggatatg ttgcgccaaa tcaaccttga
    27781 gaatatcttc gttaactacg gtcacattat caaaatcacg cagggtatct gccaaaattg
    27841 gcaccaaacg gtggtcaatc tcaaaagtca tgacttgggc tgcacgctca gccagaaact
    27901 ccgtcaaggc accaatacct ggcccgattt cgatgacatt gacctggtca tcaatttcag
    27961 ccgtatccac aattttttga aggatattgg tatccgtcaa gaaattttgc ccgaaggact
    28021 ttttaaaggt aaaaccgtga cgctccagca ctgccttggt cacgctataa tctgcaattc
    28081 tcatctattc tcttttctat tatctgttac tactattgta acacattccc cttgtatttt
    28141 gggaatgaaa atcataccaa aaaactatct ttcctcatga agaaagatag tcttggaaat
    28201 ctttaaagag ggacttagcg cttctctcga aacatacgtt catactcttc gaaaatctct
    28261 tcaaaccacg tcagcttcac cttgccgtac tcaagtacag cctgcggcta gcttcctagt
    28321 ttgctctttg attttcattg agtatcaaaa aaatcagctt cttgataact tctattttcg
    28381 ggtagggtca aatccacatc ctcctccaca tcatccactg tcagttcaac cgtttgaggt
    28441 aaatttttca aacgataatg cttttgcctt tacccttcaa tactagcaaa atcaagcaaa
    28501 tagccatgca ctaggatttg aaccaaaagg agatgattgg tcatattttc catgatgagg
    28561 atatcttcta aaccacctct ggcactagga taatcagata ccctgcgaat acggattttt
    28621 tcacacatga gcaagtttcc tcatatcaat gatttcatca gccttattcc agactagggg
    28681 attatgggtc gcaatgatga taattcgatt ttcatctttc aaatccacca agagattcat
    28741 aacctcctct gaattttcag gatcaagagc tgctgttggt tcatctgcca aaatcaaggg
    28801 tggatttttc aaaatagtct tagcaagggc aactcgttgg gcctctcccc cagataaagt
    28861 atagattttt tgttctaaat ccaaataccc tagattaact ttttctaaag cccccacttg
    28921 cctttccaaa cgttctactt ttgagatttt ctgaccaaca aaacccaaat ccaaattttc
    28981 tttgattgat tggttttcta agaggccaaa attttgaaag agatagccca tctgatctcg
    29041 aaaatactca cgagtgggaa tggtttttaa atctttcccc tgatagagaa cccttcctcc
    29101 atctatcttt tctagctttc ccaagatatt cagcagcgtc gtctttccgc ttccactctt
    29161 tccgattaag gcataaacct tgcccttctc cagcttgaga ttcaaaccag agaaaatcgc
    29221 gcggtcatta aatttctttt ccaatccttg aatatcaatc atcttatttt cctttcaata
    29281 ctgccatgct accagcttct tcttttttgt cctgccttac taaaatcaag agggcgttaa
    29341 gcgtaaacaa agctacaact agagcgctaa tcaaaccatc tcttgtcaaa atactagata
    29401 ggactaggcc aagcaagaga actcctcctt gcgccagtaa atacttgcca tgaagctcat
    29461 agattgtcat accagcaaga cgtttaatca taagctcccg tctgaactgc tcaaaataga
    29521 gaagattcat ggaatcaaat aacaagatag ccgtagatag ggtcaaaatc gtcccaataa
    29581 ggagagagta aaactccacc cgtttgtcat ttagtacctt ggcaaaaaat agctggcttt
    29641 ttaccaagta agaaaccttg tgatacagac cttgctcttt tagagctgtg atgctgtctc
    29701 catatcgatc tagtttcaag gcactattag ccgtagttcc ccacaacatc cctgcaacag
    29761 gtcttgttcc agtcgcttgc ggcgttagaa ctacaataat cggatctttt aggtactggc
    29821 gtgttgtctt gtacccatca ttatagagga aacgttcctg tcctgtttct gtaaaagcta
    29881 gccttacctg tgggaggtag tatttctgac tcgtcacttc tacactttca gatgaaaagt
    29941 tttgcaggta atctgtaaac aatccttggt agtaggtaga ctgctctcgc aagctctcag
    30001 gcaagatcag cccaaactct ccctcagaca agttttgcat cttgtccata aactctgaag
    30061 aaacggtaat cttttcttct atcagatagc gtggtgagac atagataaca ttccctgacg
    30121 gtgtgtagtc actgagacga ttgccatcta ggtccacttc tgctccatct gagaaattgt
    30181 caacattgct cataatataa aaagagtctg tattggctaa ccgttcttca gtaaatgtct
    30241 gccactcacg attatcctta cgcgttcctt cttcatcggc aaatgcacta gaccaaccaa
    30301 aggatagacg gtaacggtct gaggactggc tccatttatt gctagctctc tccatttcct
    30361 gcatttcacg gtagtggggt aggagagctg tcgcactcga tccgaccacc aatacagcta
    30421 agagttgccc caccatcatc aatgtcatca tacgtttgag agggagtttc cctttcaata
    30481 gatccaccag actattttcc tgtaaaccaa gtagatagac gacacttagt aaggtagaaa
    30541 tccctgccag ggtcaatcca taaagtagaa gagcaatgat gaccagttgc accgttgcca
    30601 taaacaaggc accttgatac cagagaatcc ccaatcccaa aagactggat accagcactg
    30661 agcagataag ctgtctcaca tcttctaaca ctggtctgag agcaactcca aacaagctct
    30721 caccagctat taagcgaatc cctgcctgac gaagggattt gatccgataa atcagggtca
    30781 gactcataaa ggtcagcaga aaaatagcta aactcagtag caccataggg gtggccgtca
    30841 atagtaagac tatcgaaaat ggatcttctc cattcgaaac aaagccttga tagccaagtt
    30901 ctttcaaggt ggtctgtaag ctcactccat ccaaacttcc tgacacaatc aagtaagacc
    30961 ctaataaatc actcgtttct gcactctcct tggaggaaat ggtcagacct tctggaagct
    31021 ctccctcacc ataagtggca taggtaaaga gtgtttctcc agcttcattt ggctcaacga
    31081 ttcgacgggc gatgagacta ttatgctcta ccgccaagtg actcaaagcc tctgtcacct
    31141 cttcgcgact agctactggt tgatttatca tcattcctgt cacatcaaca gaaggatagt
    31201 taccaaataa aatctcttct cgatggtcaa cggaagttat caccatataa agcgatacca
    31261 ataccattga aatcaaaata aataaacgtt tcatcatttc ctccaaaaag ttacttggtc
    31321 acttagtgag aaggtgacca cagtcactgc gatgttaaga agcttattaa gttctttctg
    31381 ctagcaacat cttcaaatat attatatccg ttttttcaag aactatcaag gacatcaatg
    31441 tccttaaaat ctaactattt catattattt ttagcttata tctaaaaaaa cagttctaaa
    31501 gacgaacttt agaactgttt tgatacgagg acaaggacat ttttgtcact gattcactat
    31561 gtttataaat attttttgac atcctcttgc cattcctctg tcaatttctt tactagaaat
    31621 tcatctccaa ttagctgcgc aaatgtttta gaagactgat aaaatgattc agctaaacta
    31681 aaatctcttt ctactcttaa tgcttgcttc cacaaaaaca taaatacaag aggtttcttt
    31741 tgataatctt gagttttatc cataatttct tgtaaacacg atagataatc attaaaacaa
    31801 tcagttactc ctaccttttc aagacaacac attcctgaaa taagagtatc tctaattata
    31861 aagtaattct ctatttgagt taccttcttc tgttccaaaa gaaagagcat tagcttttcg
    31921 atttgattta taaatttggc aagatctttt ctaatgagca tttgataaaa aaacaaacga
    31981 atcaacaaca tatcatttat tgaataagct tctttttcta taatctgagg catatgttcc
    32041 tcaagtatta actctgcata ttcaggcctt ctagttctag aagtatccaa acacgcctgc
    32101 aatacttcac atgctaattg ttcttcttta ggaagattat caaagtatgt tttataaatc
    32161 tcttccaaac acgcttcctt cttatcgtac tcctcttctt taccatagat tggttcacgt
    32221 aaaatctggt atttcaattc taaataagca gaaggaagag ctgaaaaatc cggcataagg
    32281 ctataaactg gaactcctag ccggcgagca atatagtcta acttgggcaa actcggttgc
    32341 gaagctccac tttcaattcg cgacagttga cgaactgtca gttcttgctc atctccacaa
    32401 aaatctggac gactaattcc tttttctatc cgaatctctt taattttttg accaattaac
    32461 atcagacgcc tcctcttcta cctgcttcga aaaatactgt gaaacttaaa aaattgtaac
    32521 ggctacatct ttcctactca taagatttca tagcttcttc cacttctgcc aaagtaaccc
    32581 caaacaactc taggcgtttg aggagttgct tgccgttgga atagccgatt cggagagtct
    32641 ctccgagata ttctctacgc ttacggctgt ctgcccctgc tagaaaacca aggcgaatca
    32701 aatcgctacg actaatgtca aactgactct catgttcaaa ttgttctgtc acctgagcta
    32761 gagccgtttt caggtcttca tagctggcat gctcaattcc cagagaacgc cccttggtct
    32821 tggactttgg aacagcttca tctcgcttga gaaaggcatg ccgaactgtt ggaatggccg
    32881 tcataatcat gcgccgaatc cgctccccat taaaatctgg gtctgtaaag acaatgactc
    32941 catgacgttg gtgcaggcgc tgaatccgct ctatatcctg agcattgatg gcagaacctc
    33001 gagtctcata ggtctccaca tcgaaataac gtttgagatt gaccgtatca tcacgccctt
    33061 caaccacgat aacttgagaa attttctctt tcattacttg ctgtccaatc caaaaattcg
    33121 ttctgcattt gcagtcgttg ctaccgccag ctcttctgtc gtcataccac gcaagtcagc
    33181 gataaagtcg accacatagc gagtataggc tgttttattt tcacgaccac gcttgggtac
    33241 aggtgctaag taaggcgcat ctgtttccac caaaatcttg tccaaaggta actctttagc
    33301 tgcttcttgg aggtcagttg ccttcttaaa agtcaccact cctgagaagg aaatggtcat
    33361 accaagatcc acaaacttct ctgcccactc aagcgtccct gaaaatgaat gcatgatacc
    33421 accacgagga ccaacgccct cactcttgat aatctcatag gtatcttcca gcgcatcacg
    33481 ggtatggaca acaaaaggca aatccaagtc cttagatagc tgaatctgac ggcgaaaaac
    33541 ctgctcctgc acctctttgg gcgctgtcat ccaatggtaa tctaagccaa tttcacctaa
    33601 agccacaacc ttgggatgtt ttaacttatc caacaagtaa gcctcaactt cctctgtata
    33661 agtaccagct tctgtaggat gccaaccaat agtcgcatag agctgatcat actcatctac
    33721 caactccaag gcacgctcaa tcgtcggttt atcaaaacca acaatattca tctgtgtcac
    33781 acccatctca gcagccaagg caatttcttc tgcctcacga cctgcaaatt cttctacatt
    33841 caagtgtgta tgtgtatcaa aaatcatctc ttctaacctc gttttctatc ttctattata
    33901 ccaaaaaaga ggaggggcac ctaatttttc ggtttcccct cctctcttca atagagagct
    33961 attctgctat cttttctatc cgatattgcc catctcctat tccacagtta gagacagaag
    34021 agattggcta cattgataga atattcctat tcagctaaag tatgtgatta tcttgggttc
    34081 tcacttttct cacccatcac taggattgat gacttctcaa aatccccctt ataatctaac
    34141 tttaagtaga gaatagaatt ttctagctgt tctattttct acgtctagtc tttcttccta
    34201 gcaaagcaaa accacttgtt gccaccacta gaccaagact agcaagtgct gcacttgatg
    34261 ctgaacctgt gttaggtaat tgtttgccag caggtgactg gtaggtaaca tcttgcattg
    34321 ttcctgcagt tgcttgcggt gcacttgttg tcttaggagc cactggtctc ttaggaactg
    34381 taaccatgac aatggcttgc actccaatcg tttctgcttc cttcacatct tccatagttt
    34441 tcgcattttc aatctctttg agagctgctt gtttttctgc ttccactctt gctaaaagtt
    34501 ctgctttttc tttatcagaa agcggtgcgc ctttgatttc atcctgcttg tccttggctg
    34561 ctttttcaat ggcattttta gctgattctt tttcagtagc taattgctta gaagcttgtg
    34621 attgagctga gttttgtgaa cctgtgtgag ctgatccacc cgcctggcta ccaccgcctg
    34681 cattcgcatt agcatctgag cttccagtat tgcctccctt atctccacct ggtgtatttt
    34741 ctagcttata ctctggtgta gcttcttgag ttacagattc acctttcgca gattgttgaa
    34801 ctgtatcttt tcctagaatc gtcactactg aaccatctgg gaatgtgatt gttgctgtac
    34861 catctccagc tacattgatt gtcgctccgt ctaatgctga accatttgct tgtaaaatag
    34921 caaccttaac tttttcttca tctgttaaat gagttatatc ttttacatct actctttgag
    34981 caggtgcttt aacagttgat ttttcagcag tcggtttagt gctatcttgt gcttttggat
    35041 cttgtgctac caattgattt cctggaattg taatagctga accatcttta aagacgattg
    35101 tcgctgtgcc atctccagct acttcaactt tatagtaaaa tgaaataata acaggacaaa
    35161 tcgatcagga cagtcaaatc gatttctaac aatgttttag aaatagaggt gtactattct
    35221 agtttcaatc tactataaca tcgcttccga atccatttgc atctactgaa cttttttaca
    35281 aaaataaaaa tcagtagact cgagttccta ctgatttttg atagaattcg tttggattat
    35341 gcagccaaaa ctttgcgtcc tttacgacga cgagctgcca atacgcgacg accattttta
    35401 gttgacatac ggttacggaa tccgtgtttg cgcgcacgac gaagtttact tggttgataa
    35461 gtacgtttca cgatgaatac ctcctcatag attttgtatt cgtttagccg gctataaagt
    35521 gatcagttac taaacatact ttactattct atctgattct ttcacttttg tcaatagctc
    35581 taggcaaatg tttccagctt tttcgaatgc aagccctatc tacgatactg tttcaagaaa
    35641 cgagtcatct tttcttggat ttgggctaac tcatcaacac gaggaaggta aacgatacgg
    35701 aagtggtctg gttcctgcca gttaaagcct cgaccatgaa ccaagagaac cttttcctgc
    35761 ttcaagaaat caaggacaaa ctgctcatca tcatcgatac ggtacatatt gcggtcgatt
    35821 tttgggaaga tatagagccc cgccttgggt ttaacggcag acaaacctgg aatatcttga
    35881 atggcattat agatgaaatt tctttgctcg tagattcgtc caccaggaag aagcaattca
    35941 tcgactgatt ggtgaccccc caaggaagtt tgtacgactt gttgggccaa aacgttagag
    36001 caaaggcgca tattggacag catattgagc ccttcgatat agcccttaac atgagtctta
    36061 gggccagaca agaccatcca acccacacgg aaacctgcta tgcggtggga ttttgacaga
    36121 ccattcatgc tgacacagaa gacatctggt gccaagctcg ccacaggcgt atgcacatgt
    36181 ccgtccatta ccatgcggtc atagatttca tccgcaaaga taatcaaatc attttgacgg
    36241 gcaatctcaa taatctccag caagagttcc ttaggataaa gggctccagt tgggttattt
    36301 ggattgataa ggacaattgc cttggtattg gaagtgattt ttgacttgat atcgtcaata
    36361 tctgggtacc attccacagc ttcatcacag atatagtgaa cggcatttcc tccagctagg
    36421 ctgacagccg ccgtccatag aggatagtct ggcattggca ctaagacctc atccccattg
    36481 tccaaaagcc cctgcatgga catgacaatc aactcactga caccatttcc aaggtaaata
    36541 tcatcaatat ctacgtttgg gaatttcttc agctggcaat actgcatgat ggccttacgg
    36601 gctgagaaaa tccctttgga gtcagaatag ccttcactat cacgcgcatt cataatcaag
    36661 tcatgaatga cctcgtctgg cgctgtaaag ccaaattctg ctggatttcc tgtattcaga
    36721 cgtaaaatct tttctccatt cgctcgcatc cgcatggctt cttccaatac aggaccacgg
    36781 atatcataag caacatgctc taacttacta gacttgttat attctttcat cttgtttcct
    36841 caattcatca ggatagtaac attataccac tagaaagaga atgcgacaag tttactgaaa
    36901 tgtgttacta gtttcgtata gaagaaaaac cttgctaaag gcgaggttaa gaacgtagag
    36961 atagcaggta ttttgcaaag acacatcaca taccaaaaga taaacctaga aaaagcaagc
    37021 ctagaaagga gtcataaaga agattaaaca tgaggaaaag cccccatatc atcaagtaat
    37081 cccaacgccc acttcgtttt gcaactagaa ataagattag atggtagagt acatgaaaga
    37141 ctaaaaatcc aataaaactt ggataaagaa gcggtaccag actctctaat tgccagatca
    37201 tgatcacttc taccaagact gaaactagga taattccata ataaaggaaa tttgattttt
    37261 gaaaagagtt tttcatcatt ctcccttcct t
//
LOCUS       DAVMHG010000005        32659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMHG010000005 DAVMHG010000000
VERSION     DAVMHG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.005"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            755..1207
                     /locus_tag="VYR08_000189"
     CDS             755..1207
                     /locus_tag="VYR08_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3465779.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFIYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1226..2563
                     /locus_tag="VYR08_000190"
     CDS             1226..2563
                     /locus_tag="VYR08_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV3465780.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2642..3526
                     /locus_tag="VYR08_000191"
     CDS             2642..3526
                     /locus_tag="VYR08_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3465781.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3526..4359
                     /locus_tag="VYR08_000192"
     CDS             3526..4359
                     /locus_tag="VYR08_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3465782.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4554..5657
                     /locus_tag="VYR08_000193"
     CDS             4554..5657
                     /locus_tag="VYR08_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3465783.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            5831..6529
                     /locus_tag="VYR08_000194"
     CDS             5831..6529
                     /locus_tag="VYR08_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3465784.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            6548..8065
                     /locus_tag="VYR08_000195"
     CDS             6548..8065
                     /locus_tag="VYR08_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3465785.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            8190..9518
                     /locus_tag="VYR08_000196"
     CDS             8190..9518
                     /locus_tag="VYR08_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3465786.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            9770..10636
                     /locus_tag="VYR08_000197"
     CDS             9770..10636
                     /locus_tag="VYR08_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3465787.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            10652..11491
                     /locus_tag="VYR08_000198"
     CDS             10652..11491
                     /locus_tag="VYR08_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3465788.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            11763..12215
                     /locus_tag="VYR08_000199"
     CDS             11763..12215
                     /locus_tag="VYR08_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3465789.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            12312..12656
                     /locus_tag="VYR08_000200"
     CDS             12312..12656
                     /locus_tag="VYR08_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465790.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            12940..13584
                     /locus_tag="VYR08_000201"
     CDS             12940..13584
                     /locus_tag="VYR08_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3465791.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            13753..14670
                     /locus_tag="VYR08_000202"
     CDS             13753..14670
                     /locus_tag="VYR08_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV3465792.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            14688..15572
                     /locus_tag="VYR08_000203"
     CDS             14688..15572
                     /locus_tag="VYR08_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3465793.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(15588..16439)
                     /locus_tag="VYR08_000204"
     CDS             complement(15588..16439)
                     /locus_tag="VYR08_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3465794.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            16639..18696
                     /gene="pbp2b"
                     /locus_tag="VYR08_000205"
     CDS             16639..18696
                     /gene="pbp2b"
                     /locus_tag="VYR08_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV3465795.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            18707..19303
                     /gene="recR"
                     /locus_tag="VYR08_000206"
     CDS             18707..19303
                     /gene="recR"
                     /locus_tag="VYR08_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV3465796.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            19479..20522
                     /locus_tag="VYR08_000207"
     CDS             19479..20522
                     /locus_tag="VYR08_000207"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV3465797.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            20606..21979
                     /locus_tag="VYR08_000208"
     CDS             20606..21979
                     /locus_tag="VYR08_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV3465798.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            21966..22577
                     /locus_tag="VYR08_000209"
     CDS             21966..22577
                     /locus_tag="VYR08_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3465799.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            22668..23372
                     /locus_tag="VYR08_000210"
     CDS             22668..23372
                     /locus_tag="VYR08_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV3465800.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            23591..24964
                     /gene="ftsA"
                     /locus_tag="VYR08_000211"
     CDS             23591..24964
                     /gene="ftsA"
                     /locus_tag="VYR08_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV3465801.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            24981..26240
                     /gene="ftsZ"
                     /locus_tag="VYR08_000212"
     CDS             24981..26240
                     /gene="ftsZ"
                     /locus_tag="VYR08_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV3465802.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            26245..26916
                     /locus_tag="VYR08_000213"
     CDS             26245..26916
                     /locus_tag="VYR08_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV3465803.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            26926..27465
                     /locus_tag="VYR08_000214"
     CDS             26926..27465
                     /locus_tag="VYR08_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV3465804.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            27465..27728
                     /locus_tag="VYR08_000215"
     CDS             27465..27728
                     /locus_tag="VYR08_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV3465805.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            27725..28510
                     /locus_tag="VYR08_000216"
     CDS             27725..28510
                     /locus_tag="VYR08_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV3465806.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            28519..29307
                     /gene="divIVA"
                     /locus_tag="VYR08_000217"
     CDS             28519..29307
                     /gene="divIVA"
                     /locus_tag="VYR08_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV3465807.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            29655..32447
                     /locus_tag="VYR08_000218"
     CDS             29655..32447
                     /locus_tag="VYR08_000218"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV3465808.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         9971 a         5833 c         7126 g         9729 t
ORIGIN      
        1 accttgtctt ctcctaaaat tgactttctt gttttcttat cttgtccact cgaaacaagt
       61 attgtaagaa tttgattatt tttgaaagta cttttaatat acttgatata gttaaaaaag
      121 atttgaaact aaattccaaa ttagaaaaag acttgaaata ctaaaaaaaa aaagtatact
      181 ctaattgaaa acggtaacaa aactaattta gagaatgaaa tatagagtat ttctctctta
      241 aaagtttttg gtgaaacgag atgtagaaag gagatttagc caaagagtct attagtgcta
      301 gaataataga ttagaattat tttagaaaaa cgaagtgagc agcttataaa ttcaagtccc
      361 caaatagatt catactagta tcttttgcaa aaaataaagg gcgacttcct tcatgaatat
      421 caatttcatc tataaggaag gtagctaatt gaactaactt atttattctg tttgtcgcta
      481 gaaaaatcag acctccttgt gaagattgag gagatactga atgaaaatca aagaagaaac
      541 tagcaagcta gtagcagatt gcccaaaaca ccgctttgag gttgtagata agactgacgt
      601 atataatcca aggtgaagcg actgtggttt gaagagattt tcaaagagta taggctagag
      661 agtagtgttt ttatgtcctt ctagtagaaa atgctagaca gaagaatggg gaacttggat
      721 aggaaaaata gattgagaaa ggaggttaga agagatgatt attacaaaaa ttagccgttt
      781 aggaacttat gtgggagtca atccacattt tgcaacatta atagattttc tagaaaaaac
      841 aggactagaa aatttaacag aaggttcgat tgctatcgat ggtaatcgat tgtttgggaa
      901 ttgctttatt tatctagcag atggtcaagc aggggctttc tttgaaacac accaaaaata
      961 tttggatatt catttagttt tggaaaacga agaagccatg gctgttacat cgccggaaaa
     1021 tgtaagcgtt acccaagaat atgatgaaga gaaagatatt gaattataca cagggaaagt
     1081 ggaacagttg gttcatttga gagctggtga atgcctcatc acttttccag aagatttaca
     1141 tcaacccaag gttcgtataa atgatgaacc tgtgaaaaaa gttgtcttta aagttgcgat
     1201 ttcttaatgt agaaagagaa gaacgatgaa aaaaatgaga aagtttttat gtctagctgg
     1261 aattgcgcta gcggctgttg ccttggtagc ttgttcagga aaaaaagaag ctacaactag
     1321 tactgaacca ccaacagaat tatctggtga gattacaatg tggcactcct ttactcaagg
     1381 accccgttta gaaagtattc aaaaatcagc agatgctttc atgcaaaagc atccaaaaac
     1441 gaaaatcaag attgaaacat tttcttggaa tgacttctat actaaatgga ctacaggttt
     1501 agcaaatgga aatgtgccag atatcagtac agctcttcct aaccaagtaa tggaaatggt
     1561 caactcagat gctttggttc cgctaaatga ttctatcaag cgtattggac aagataaatt
     1621 taacgaaact gccttaaatg aagcaaaaat cggagatgat tactactctg ttcctcttta
     1681 ttcacatgca caagtcatgt gggttagaac agatttgtta aaagaacata atattgaggt
     1741 tcctaaaact tgggatcaac tctatgaagc ttctaaaaaa ttgaaagaag ctggagttta
     1801 tggcttgtct gttccgtttg gaacaaatga cttaatggca acacgtttct tgaacttcta
     1861 cgtacgtagt ggtggaggaa gcctcttaac aaaagatctt aaagcagact tgacaagcca
     1921 acttgctcaa gatggtatta aatactgggt taaattgtat aaagaaatct cacctcaaga
     1981 ttctttgaat tttaatgtcc ttcaacaagc taccttgttc tatcaaggaa aaacagcatt
     2041 tgactttaac tctggcttcc atatcggagg aattaatgcc aacagtcctc aattgattga
     2101 ttcgattgat gcttatccta ttccaaaaat caaagagtct gataaagacc aaggaattga
     2161 aacctcaaac attccaatgg ttgtttggaa aaattcaaaa catccagaag ttgctaaagc
     2221 attcttagaa gcactttata atgaagaaga ctacgttaaa ttccttgatt caactccagt
     2281 aggtatgttg ccaactatta aggggattag cgattctgca gcctataaag aaaatgaaac
     2341 tcgtaagaag tttaaacatg ctgaagaagt aattactgaa gctgttaaaa aaggtactgc
     2401 tattggttat gaaaatggac caagtgtaca agctggtatg ttgactaacc aacacattat
     2461 tgaacaaatg ttccaagata tcattacaaa tggaacagat cctatgaaag cagcaaaaga
     2521 agcagaaaaa caattaaacg atttatttga ggctgttcag tagatgtaaa agactagaaa
     2581 ataggtgggc tagtgatctg aaaagctcta gcccaatttt gtaaaagaag ggagaaggag
     2641 aatggttaaa gaacgtaatt taactcgctg gatatttgtt ttgccagcta tgattatcgt
     2701 aggattactc tttgtttatc cgtttttctc gagtattttt tatagcttta ccaataagca
     2761 tttgattatg cctaattata aatttgttgg tttggctaac tataaagctg tgctatcaga
     2821 tcccaacttc tttaatgcgt tctttaattc aattaagtgg accgttttct cattagttgg
     2881 tcaagtttta gtagggtttg tgttggcttt agctcttcac agagtacgcc acttcaagaa
     2941 attatatagg accttattga ttgttccttg ggcatttcct accatcgtta ttgccttctc
     3001 ttggcagtgg attctaaacg gggtttatgg ctacttacct aatctaatcg taaaattagg
     3061 tttaatggaa catacacctg catttttgac agatagtaca tgggcattcc tatgtttggt
     3121 gtttatcaac atttggtttg gagcaccaat gattatggtt aatgtgcttt cagctttgca
     3181 aacagtacca gaagaacaat ttgaggctgc taagatagat ggtgcttcaa gttggcaggt
     3241 gttcaagttt atcgtctttc cacatattaa agtggttgta ggacttctag ttgttttgag
     3301 aactgtatgg atctttaata actttgacat tatctacctc attactggtg gtggaccagc
     3361 caatgctaca acgacgcttc caatttttgc ttacaacctg ggctggggaa ctaaattgtt
     3421 gggtcgtgct tcagcagtta cagtattgct ctttatcttc ttggtggcga tttgctttat
     3481 ctactttgct atcatcagta agtgggaaaa ggagggtaga aaataatgaa gaagaaatcc
     3541 agtatttatt tagatattct ctcacatgta cttttagttg gtgcgaccat cgttgcaatt
     3601 ttcccattgg tatggattat catatcttct gtcaaaggga aaggggaatt aactcagtat
     3661 ccaacacgat tttggcctga acagtttaca ttagattatt tcactcatgt tatcaacgat
     3721 ttgcacttca ttgataacat tcgaaacagt ttaatcattg ccttggctac aaccctcatt
     3781 gcgattatta tttctgctat ggcagcctat ggtattgttc gattctttcc taaattggga
     3841 gcaatcatgt cgagactact cgtcattacc tacattttcc caccaatttt gttagcaatt
     3901 ccctattcaa ttgccattgc taaagttggg ttaacaaata gtttatttgg cttgatgatg
     3961 gtttatctat cttttagtgt tccatatgca gtttggctct tagttggatt tttccaaaca
     4021 gttccaattg gaattgaaga agcggctaga attgatggtg caaataaatt tgttacgttt
     4081 tataaagttg tgctaccgat tgtagcacca ggtattgtag caacagctat ttatacattt
     4141 atcaatgctt ggaatgaatt cctgtatgcc ttgattttga ttaacaatac aggaaagatg
     4201 acagtagcag tagcccttcg ttcacttaat ggttcagaaa tactagactg gggagatatg
     4261 atggcagcgt ctgttattgt agttcttcca tcaattattt tcttctctat catccaaaat
     4321 aagattgcaa gtggattatc agaaggatct gtgaagtaat actcaatgaa aatcaaagag
     4381 caaactagga aactagccgc aggctgctca aagtacagct ttgaggttgc agataaaact
     4441 gacgaagtca gtaaccatac gcacagtaag gcgacgttga cgcggtttga agagattttc
     4501 gaagagtata agttaaaatt agaaagttta ttcaataata aaggagaaaa aacatggtta
     4561 aatacggtgt tgttggagca gggtattttg gagctgaatt ggctcgctac atgcaaaaga
     4621 atgatggagc agagattact cttctctatg atccagataa tgcagaggcg attgcagaag
     4681 aattgggagc aaaagtagca agttccttag atgagttggt ttctagcgat gaagtagatt
     4741 gtgttatcgt cgcaactcca aataatcttc ataaggaacc ggttattaag gctgcacagc
     4801 atggtaaaaa tgttttctgt gaaaaaccaa ttgcgctttc ttatcaagat tgtcgcgaga
     4861 tggtagatgc gtgtaaagaa aacaatgtaa cctttatggc aggacatatt atgaatttct
     4921 ttaatggtgt tcatcatgca aaagaactca ttaatcaagg agttatcgga gacgttctat
     4981 attgtcatac agctcgtaat ggttgggaag aacaacaacc gtcagtgtca tggaaaaaaa
     5041 ttcgtgaaaa atcaggtggt cacttgtatc accacatcca tgaattggat tgcgttcaat
     5101 tccttatggg gggcatgcct gaaactgtaa ccatgacagg tggaaatgtg gcccatgaag
     5161 gtgaacattt cggtgatgaa gatgatatga tttttgtcaa tatggaattt tctaataagc
     5221 gttttgcctt gttagaatgg ggttcagctt atcgttgggg tgaacattat gtcttaatcc
     5281 aaggaagcaa aggtgccatc cgcttagact tattcaactg taaaggaact cttaagctag
     5341 atgggcaaga aagctatttc ttgattcacg aatcgcaaga agaagatgat gatcggactc
     5401 gtatctatca tagtacagag atggatggag caattgctta tggtaaacca ggtaaacgta
     5461 ctccattatg gctatcatct gtcattgata aagaaatgcg ctatctgcat gagattatgg
     5521 aaggagcccc agtatcagaa gaatttgcaa aacttttgac aggtgaagct gccctagaag
     5581 caattgctac tgcagatgct tgtacccagt ctatgtttga agatcgcaaa gtaaaattgt
     5641 cagaaattgt aaaataaatt ttggtattct cctatttata ggtcgacttg ctcctctgaa
     5701 agtactttta gaggagctgt ttgactttgc tagtttttga aactgaaatc tattatacta
     5761 caaactattg aaagcgtttt aattttaagg tataataatc tcatagaaat aaagaaaagg
     5821 aggaaagagg atgccacaga ttagcaaaga agccttgatt gagcaaatca aagatggaat
     5881 catcgtttct tgtcaggctc ttcctcatga accgctttat acagaagcgg gaggggtgat
     5941 tcccttgctg gtcaaagcgg ctgagcaagg tggagcagtc ggtatccgag caaacagtgt
     6001 tcgcgatatc aaggaaatta aggaagtcac taaacttcca atcattggga ttatcaaacg
     6061 tgattatcca cctcaggaac ccttcatcac ggctactatg aaagaagttg atgaattggc
     6121 agaactggac atcgaggtga ttgctctgga ttgtaccaag cgtgaacgct acgatggttt
     6181 ggaaattcaa gagttcattc gtcaggttaa ggagaaatat cctaatcagc ttttgatggc
     6241 tgatactagt atcttcgaag aagggctagc agctgtagaa gcaggaattg actttgtcgg
     6301 aacaacctta tcaggctaca catcctacag tccaaaagta gacggtccag attttgaatt
     6361 gattaagaaa ctctgtgatg ccggtgtaga tgtcattgca gaaggaaaaa ttcatacacc
     6421 agaacaagcc aaacaaatcc ttgaatatgg agtgcgaggc atcgttgttg gtggcgccat
     6481 tactagacca aaagagatta cagaacgctt cgttgctagt cttaaataag atgtgagagg
     6541 gagttttatg tttaaagttt tacaaaaagt tggaaaagct tttatgttac ctatagctat
     6601 acttcctgca gcaggtctac ttttggggat tggtggtgca ctttcaaacc caaccacgat
     6661 agcaacttat ccaatactag acaatagtat ttttcaatca atattccaag taatgagctc
     6721 tgcaggagag gttgtattca gtaatttgtc actacttctc tgtgtgggat tatgtattgg
     6781 cttagcgaaa cgagataaag gaaccgctgc gttagcagga gtaactggtt acttagttat
     6841 gactgcaacg atcaaagctt tggtaaaact ttttatggca gaaggatctg caattgatac
     6901 tggagttatt ggagcattag ttgtcggaat agttgccgta tatttgcaca accgatataa
     6961 caatattcaa ttaccttccg ctttaggatt ctttggaggt tcacgcttcg ttcctattgt
     7021 tacatcgttc tcttctatct tgattggctt tgtcttcttt gttatttggc cacctttcca
     7081 acaacttctt gtttctacag gtggatatat ttctcaggcg ggtccaattg gaacttttct
     7141 atatggattt ttaatgagac tttctggagc agtaggctta catcatataa tttaccctat
     7201 gttttggtat actgaacttg gtggtgttga aactgttgca ggacaaacag tggttggagc
     7261 tcaaaaaata ttttttgctc aattagccga tccggcccat tctggattat ttacagaagg
     7321 aacaaggttt tttgcaggtc gtttctcaac aatgatgttc ggtttaccgg ctgcctgttt
     7381 agcgatgtac catagtgttc ctaaaaatcg tcgtaaaaaa tacgcgggtt tgttttttgg
     7441 agttgcttta acatctttta ttaccggtat tacagaacca attgaattta tgtttctatt
     7501 cgtcagtccg gttctatatg ttgttcacgc attccttgat ggtgttagct tctttattgc
     7561 agacgtctta aatatttcaa taggaaacac attttcagga ggtgtaatcg atttcacttt
     7621 atttggaatt ttgcagggga acgctaagac gaattgggtt cttcagattc caattggact
     7681 tatttggagt gttttgtatt atattatttt tagatggttc attactcaat tcaacgttct
     7741 aacgccagga cgaggagaag aagtagattc taaagaaatt tctgaatccg cagattcaac
     7801 ttcaaatact gcagattatt taaaacagga tagcctacaa attatcagag ccttgggtgg
     7861 atcaaataat atagaagatg tagatgcttg tgtgacacgt ttacgtgtag ctgtaaaaga
     7921 agttaatcaa gttgataaag cacttttaaa acaaattggt gcagttgatg tcttagaagt
     7981 gaagggtggc attcaagcaa tatatggagc aaaagcaatc ttatataaaa atagtattaa
     8041 tgaaatttta ggtgtagatg attaagtact tactgactta ataaaaaaca gaggagagtg
     8101 atggatgagt aggatgaaat gaaatcgcat acaagaaata aagaactcat tatccaagtt
     8161 ggatacgctt attacatagg agaatacaaa tgaaatttag aaaattagct tgtacagtac
     8221 ttgcgggtgc tgcggttctt ggtcttgctg cttgtggcaa ttctggcgga agtaaagatg
     8281 ctgccaaatc aggtggtgac ggtgccaaaa cagaaatcac ttggtgggca ttcccagtat
     8341 ttacccaaga aaaaactggt gacggtgttg gaacttatga aaaatcaatc atcgaagcgt
     8401 ttgaaaaagc aaacccagat ataaaagtga aattggaaac catcgacttc aagtcaggtc
     8461 ctgaaaaaat cacaacagcc atcgaagcag gaacagctcc agacgtactc tttgatgcac
     8521 caggacgtat catccaatac ggtaaaaacg gtaaattggc tgagttgaac gacctcttca
     8581 cagatgaatt tgttaaagat gtcaacaatg aaaacatcgt acaagcaagt aaagctggag
     8641 acaaggctta tatgtatccg attagttctg ccccattcta catggcaatg aacaagaaaa
     8701 tgttagaaga tgctggagta gcaaaccttg taaaagaagg ttggacaact gatgattttg
     8761 aaaaagtatt gaaagcactt aaagacaagg gttacacacc aggttcattg ttcagttctg
     8821 gtcaaggggg agaccaagga acacgtgcct ttatctctaa cctttatagc ggttctgtaa
     8881 cagatgaaaa agttagcaaa tatacaactg atgatcctaa attcgtcaaa ggtcttgaaa
     8941 aagcaactag ctggattaaa gacaatttga tcaataatgg ttcacaattt gacggtgggg
     9001 cagatatcca aaactttgcc aacggtcaaa catcttacac aatcctttgg gcaccagctc
     9061 aaaatggtat ccaagctaaa cttttagaag caagtaaggt agaagtggta gaagtaccat
     9121 tcccatcaga cgaaggtaag ccagctcttg aataccttgt aaacggattt gcagtattca
     9181 acaataaaga cgacaagaaa gtcgctgcat ctaagaaatt catccagttt atcgcagatg
     9241 acaaagagtg gggacctaaa gacgtagttc gtacaggtgc tttcccagtc cgtacttcat
     9301 ttggaaaact ttatgaagac aaacgcatgg aaacaatcag cggctggact caatactact
     9361 caccatacta caacactatt gatggttttg ctgaaatgag aacactttgg ttcccaatgt
     9421 tgcaatctgt atcaaatggt gacgaaaaac cagcagatgc tttgaaagcc ttcactgaaa
     9481 aagcgaacga aacaatcaaa aaagctatga aacaatagtc cttagttatt ctataaaaag
     9541 tagtttttta aagaacctaa gagtgtatac ccccttttcc ctctacacag atagtgtaag
     9601 aaaagggggc ttttgtttaa aatgtaagaa actgtcacga aattaaaatg aagttcttac
     9661 ataagcgaac cataaaaaat ttcattttga ttttaaaaca gttcaagaaa gtcaaaaaat
     9721 tattctattt gaaagagagg tgccgactgt gaaagtcaat aaaatccgta tgcgggaaac
     9781 agtgatttcc tacgctttcc tagcaccagt attattcttc tttgtcatct ttgtgttggc
     9841 tccgatggtg atgggcttca ttacaagttt ctttaactac tcaatgacta aatttgagtt
     9901 tgtgggctta gataactata tccgtatgtt taaagatcct gtctttacaa aatctctgat
     9961 taacacagtt attttggtta ttggatctgt accagttgtt gttctattct cactctttgt
    10021 agcatctcag acctatcatc aaaatgtcat tgccagatcc ttctaccgtt tcgtcttctt
    10081 ccttcctgtt gtaacgggta gtgttgccgt gacagttgtt tggaaatgga tttatgaccc
    10141 actatcaggg attctaaact ttgtccttaa gtcaagccac atcatcagcc aaaacatttc
    10201 ttggttggga gataaaaact gggcattgat ggcgattatg attattctct tgaccacttc
    10261 agttggtcag cccatcatcc tttatatcgc tgccatgggg aatattgaca attcactggt
    10321 tgaagcggcg cgtgttgatg gtgcaactga gtttcaagtt ttttggaaga ttaaatggcc
    10381 aagccttctt ccaacaactc tttatattgc aatcatcaca acaattaact cattccagtg
    10441 tttcgccttg attcagcttt tgacatctgg tggtccaaac tactcaacaa gtaccttgat
    10501 gtactacctt tacgaaaaag ccttccaatt gacagaatac ggctatgcca acacaattgg
    10561 tgtcttcttg gcagtcatga ttgctatcgt aagctttgtt caatttaaag tacttggaaa
    10621 cgacgtagaa tactaaagaa aggagacagc tatgcaatct acagaaaaaa aaccattaac
    10681 agcctttact gttatttcaa caatcatttt gctcttgttg actgtgctgt tcatctttcc
    10741 attctactgg attttgacag gggcattcaa atcacaacct gatacaattg ttattcctcc
    10801 tcagtggttc cctaaaatgc caaccatgga aaacttccaa caactcatgg tgcagaaccc
    10861 tgccttgcaa tggatgtgga actcagtatt tatctcattg gtaaccatgt tcttagtttg
    10921 tgcaacctca tctctagcag gttatgtatt ggctaaaaaa cgtttctatg gtcaacgcat
    10981 tctatttgct atctttatcg ctgctatggc gcttccaaaa caagttgtcc ttgtaccatt
    11041 ggtacgtatc gtcaacttca tgggaatcca cgatactctc tgggcagtta tcttgccttt
    11101 gattggatgg ccattcggtg tcttcctcat gaaacaattt agtgaaaata ttcctacaga
    11161 gttgcttgaa tcagctaaaa tcgacggttg tggtgagatt cgtaccttct ggagtgtagc
    11221 cttcccgatt gtgaaaccag ggtttgcagc ccttgcaatc tttaccttca tcaatacttg
    11281 gaatgactac ttcatgcaat tggtaatgtt gacttcacgt aacaatttga ccatctcact
    11341 tggggttgcg accatgcagg ctgaaatggc aaccaactat ggtttgatta tggcaggagc
    11401 tgcccttgct gctgttccaa tcgtcacagt cttcctagtc ttccaaaaat ccttcacaca
    11461 gggtattact atgggagcgg tcaaaggata atactctgag aaaatctctt caaactacgt
    11521 cagcgtcacc ttgccatact taagtattgc ctgcggttag cttcctagtt tgttcttcaa
    11581 ttttcattga gtataggaaa atcaatctat caagatacag aagtatattt tatagattta
    11641 gagaatatag aggttataag tgtctacaaa atggagggta tgcagttact ttatgaagtt
    11701 ttgtcagaca cttataaact taagaatggt tttagttaac tatcagaaac gaaggaaaga
    11761 gtatgatttt tgacgatttg aaaaacatca ccttttacaa agggattcat cccaatttag
    11821 acaaggctat cgactatctc taccaacatc gtaaagattc attcgaatta ggaaagtatg
    11881 agattgatgg agataaagtc tttctagttg ttcaggaaaa tgtcctcaat caagttgaga
    11941 ataatcaatt tgaacaccat aagaactatg cagatttgca tttgctggta gaaggacatg
    12001 aatattcgag ctacggttca cgtatcaaag acgaggcagt agcattcgac gaagcgactg
    12061 acattggctt tgttcattgt catgaacact acccactctt gttgggttat cataattttg
    12121 cgattttctt cccaggtgag ccacatcagc caactggcta tgcaggtatg gaagaaaagg
    12181 ttcgaaaata tctctttaaa attttgattg attaaaaata ggatgaattg tttttttgta
    12241 aagctttgac aatactctac catgaaattg atctttgtga ggtagagaaa tgagaataaa
    12301 atatttaaaa attggtatct tctaagcatg ctgcaagagc tagtttctta gatggacagg
    12361 ggattacagt tgatgagatg tcctggataa ttaatggcat tgtgaatgca ttgattggta
    12421 gatacataaa attaggtact tatgcagcta agtatggtat tagtatagca cgctcgatct
    12481 taagtagggc agctgcaact gcagcagcaa gagtaggatt actgaccaag atttctggat
    12541 ggattttacg agtagctgtg aatgtagctg atgtatatgg taattttgcc aacaatattg
    12601 ctgcagcttg ggatgcatat gataaaattc ctaacaatgg tcgtataaac ttttaaaatg
    12661 cgagaatgaa agcactttgt atttttttat tgaatatgtt agcttggaca gtgcttgcaa
    12721 tgataattcg tggagggcta gatggatttg ataggcatac ttggagtact attttaattg
    12781 cttcgctgtt cggggtatat gattataagc ccatagataa aaatagaaaa aagtccaaaa
    12841 gaaaaaagaa aaaatagatt tgttcatggt agggacttat gaaagcttta ctgacaaaaa
    12901 agaaaacagt ttacaaagaa aaatgatgga ggagcaaaca tggcacaaaa aggagtaagc
    12961 cttatcaagg cagcatttga tacagataac tttctcatgc gttttagtga gaaggtcttg
    13021 gacatcgtga cagccaatct tctttttgtc gtctcttgtt tgcccatcgt gacgattgga
    13081 gtggctaaaa tcagcctcta cgagaccatg ttcgaagtta agaagagcag acgggtgcct
    13141 gtttttaaaa tctatctaag atctttcaag caaaatctga aactaggtct tcagttaggt
    13201 ctgcttgagt tgggcattgt ttcattaagc ctcctagatc tttatctctt ttggggtcaa
    13261 acagctctgc cctttcaatt gctgaaagct atttgtctag gcattctgat tttccttacg
    13321 attgtgatgt tggctagcta tccaatcgcg gcacgttatg atttaacttg gaaagaaatt
    13381 cttcaaaaag gattgatgtt ggctagtttt aactttcctt ggttcttcct catgttagcc
    13441 attcttgtcc tcattgtgat ggttctttat ctgtctgcct tcagtctact cttaggtggc
    13501 tcagtcttcc tgctttttgg gtttggacta ttggtcttta tccagactgg attgatggag
    13561 aaaattttcg caaaatacca ataggagcct tatttctgaa actactttca aaggctccaa
    13621 acgctattct ataagcgaga aactaaaatc taagtgtcta taagatattg aaagcctttt
    13681 cggtaaagtg tatactaaag aagtaaagaa aaatctgctt tagcagaaaa taaaaataat
    13741 aggagaaaat ctatgtcaga tttgaaaaaa tacgaaggtg tcattccagc cttctacgca
    13801 tgttatgatg agcaaggaga agtaagtcca gagcgtacgc gtgccttggt tcaatacttc
    13861 attgataaag gtgttcaagg tctttatgtc aatggttctt ctggtgaatg tatctaccaa
    13921 agcgttgaag atcgcaagtt gattttggaa gaagtcatgg cggtagccaa aggtaaattg
    13981 accattattg cccatgttgc ttgcaataat actaaagata gtatggaact tgctcgccat
    14041 gctgaaagct tgggagtaga tgctattgca acgattccac caatttactt ccgcttgcca
    14101 gaatactcag ttgccaaata ctggaacgat attagttctg cagctccaaa cacagactac
    14161 gtgatttaca acattcctca attggcaggg gttgctttga ctccaagcct ttacacagaa
    14221 atgttgaaaa atcctcgtgt tatcggtgtt aagaactctt ctatgccagt tcaagatatc
    14281 caaacctttg tcagccttgg cggggaagac catattgtct ttaacggtcc agatgaacag
    14341 ttcctaggtg gccgcctcat gggtgctaag gctggtatcg gtggtactta tggtgctatg
    14401 ccagaactct tcttgaaact caatcagttg attgcggata aggacctaga aacagcgcgt
    14461 gaattgcagt atgctatcaa cgcaatcatt ggtaaactca cttctgctca tggaaatatg
    14521 tacggtgtca tcaaagaagt cttgaaaatc aatgaaggct tgaatattgg atctgttcgt
    14581 tcaccattga caccagtaac tgaagaagat cgtccagttg tagaagcggc tgcagccttg
    14641 attcgtgaaa ccaaggagcg cttcctctaa tctaaaagga ggtatttatg acatactacg
    14701 ttgcaattga tatcggtgga actaacatca agtatggttt ggttgatcaa gaggggcaac
    14761 ttcttgaatc gcatgaaatg ccaactgagg cgcataaggg tggacctcat atcttacaaa
    14821 agaccaaaga tatcgtagct agttatttag aaaaaggccc agtagcaggt gttgccatat
    14881 cttctgctgg gatggtggat ccggataagg gtgagatttt ctatgctggg ccgcaaatcc
    14941 ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga aagctttact attccttgtg
    15001 agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt tcaggcaagg
    15061 gagcaagtgt gacactttgc ttgaccattg gaacaggtat cggtggttgc ttgattatgg
    15121 ataggaaagt cttccatggt tttagtaatt cagcctgtga agtcgggtat atgcatatgc
    15181 aggatggagc ttttcaagac ttggcttcta caacagcttt agtgaaatat gtagctgaag
    15241 cccatggaga agatgttgat cagtggaatg gccgacgtat ctttaaggaa gctactgaag
    15301 gaaacaaaat ctgcatggaa ggtattgacc gtatggttga ctatctagga aaaggtctgg
    15361 caaatatttg ctacgttgcc aatccagaag tggttattct tggtggtggt atcatggggc
    15421 aagaggctat cctcaaacct aagatccgca cagccttgaa agaggctttg gtaccaagtt
    15481 tagcagaaaa aacacgatta gaatttgccc atcaccaaaa tacagcaggg atgttgggtg
    15541 catattatca ttttaagaca aaacaatcct agtttggctc agccaaacta ggattttctt
    15601 acacgttttt gtctacgata gccgttgagt tttttatttt cccagtagct attaaagatt
    15661 ttttccttgc tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc gataaagaag
    15721 agcataggaa gttgagcgga tattcgttgg atataggagg gttggctgtg ggtggctaca
    15781 agaacagtct ctgtataggt ctggctatct ttattgggaa cacttgtaaa gagtacagtc
    15841 tttgccccca tctccttggc atctaataga ctatctaaaa tagaaggagt tgagcctgaa
    15901 agtgagaagc ccagtactag acaattttca tccatgatgc tggttgtcca ggcaaagccg
    15961 tcttggtctg tcaaagcttc gcagaccaca cctagtcgca taaaacgtaa tttcatttca
    16021 cgggcgacga ggccagaact ccctgttccg aagaagtaga tacgctcagc atcttcgatt
    16081 agctgggcaa ttcgttctag ttggatttcg tcaatcaagt cttgtgtttg ttccctcatg
    16141 ttgctataac ttctgaggac tcgtttggtc agtggactgt gcttggagac ttggttggct
    16201 tgattttctg cctgatgttg gtattggaaa ataaattctc ggtagccagt aaagccacac
    16261 tttttagcaa agcgggtcaa agcagcttga gaaatatgta atttttgggt gacttgttga
    16321 gaagataaat catctgtaat cgtttcagct tgcaaaaaat agcgagcgat ttcttgttct
    16381 aggtctgtca tttcttcaaa atgtgaatca atgatagttg cgatatctgg tttgtccata
    16441 gggaaagctc ctttacatga gtcatactgg aagactagat cagggaatag tcacacttca
    16501 ttataacaca taatataagg atagataaat aaaaacgcat ctctgtttta aaaacgaaaa
    16561 aatcgaaaaa gcttctctct tttccataat tttctactca aattgtggta caattaagag
    16621 taagatttta agttagaaat gagactgatt tgtatgagaa aatttaacag ccattcgatt
    16681 ccgattcggc ttaatttatt gttttcaatc gtcattttac tctttatgac cattattggt
    16741 cgtttgttgt atatgcaggt tttgaacaag gatttttacg aaaaaaagct agcctcagct
    16801 agtcagacca agattacaag cagttcagcc cgtggggaaa tttatgatgc tagtggaaaa
    16861 cctttggtag aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa taaaatgaca
    16921 gctacagact taaaagaaac agctaaaaag ttactgactt atgtgagcat cagttctcca
    16981 aatttgacag aacgccagct ggcggattac tatttggctg atcctgaaat ctataaaaaa
    17041 acagtggaag ctctcccaag cgagaaacgc ttggattcag atggcaatcg tctatccgaa
    17101 tcagaactgt ataacaatgc ggtcgatagt gtacaaacga gtcaactaaa ctatacagag
    17161 gatgaaaaga aagaaatcta tctttttagt cagttaaatg ctgttggaaa ctttgcgaca
    17221 ggaaccattg cgacagatcc tctaaatgat tctcaggtgg ctgttattgc ctctatttca
    17281 aaggagatgc ctggcattag tatttctact tcttgggata gaaaggtttt ggaaacttcc
    17341 ctttcttcta tagttgggag tgtatccagt gaaaaagctg gtctcccagc ggaagaagca
    17401 gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg taggaacctc ctatttggaa
    17461 aagcaatatg aagagacctt acaaggaaaa cgctcggtaa aagaaatcca tctggataaa
    17521 tatggcaata tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa caatatcaaa
    17581 ctgaccattg atttggcttt ccaagatagc gtggatgctt tactgaaaag ttatttcaat
    17641 tccgagctag aaaatggtgg agccaagtat tctgaaggtg tctatgcagt cgcccttaac
    17701 ccaaaaacag gtgcggtttt gtctatgtca gggattaaac atgacttgaa aacgggagag
    17761 ttgacgcctg attccttggg aacggtaacc aatgtctttg ttccaggttc ggttgtcaag
    17821 gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt caggaaacca gaccttgaca
    17881 gaccagtcca ttgtcttcca aggttcagct cccatcaatt cttggtatac tcaggcttac
    17941 ggttcattcc ctatcacagc ggtccaagct ctggagtatt catcaaatac ctatatggtc
    18001 caaacagcct taggtcttat ggggcaaacc tatcaaccca atatgtttgt cggcaccagc
    18061 aatctagagt ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg cttgggtact
    18121 gcgacaggaa ttgacctacc agatgaatct actggatttg ttcccaaaga gtatagcttt
    18181 gctaattaca ttactaatgc ctttgggcag tttgataact atacgccgat gcagttggct
    18241 cagtatgtag caactattgc aaataatggt gttcgtgtgg ctcctcgtat tgttgaaggc
    18301 atttatggta ataatgataa gggaggactg ggtgacttga ttcagcaact gcaaccgaca
    18361 gagatgaata aggtcaatat atccgactcc gatatgagca tcttgcacca aggtttttat
    18421 caggttgccc atggtactag tggattgaca actggacgtg ccttttcaaa tggtgccttg
    18481 gtatccatta gcggaaaaac aggtacagcc gaaagctatg tggcagatgg tcagcaagca
    18541 accaatacca atgcggtggc ctatgcccca tctgataatc cccaaatcgc tgtcgcagtg
    18601 gtctttcctc ataataccaa tctaacaaat ggtgtaggac cttccattgc gcgtgacatt
    18661 atcaatctgt atcaaaaata ccatccaatg aactagaaag gaaattatgc tttatccaac
    18721 acctattgcc aagttgattg acagttattc taagttacca ggtatcggga ttaagacggc
    18781 tacgcgtctg gccttttata cgattgggat gtctgctgat gatgtcaatg aatttgcaaa
    18841 aaatctcctt tctgctaaga gagaattgac atattgttct atttgtggac gtttgacaga
    18901 cgacgatcct tgttctatct gtactgattc gactcgtgac cagacaacaa ttttagttct
    18961 tgaggatagt agagatgtgg cagccatgga aaatatccaa gaataccatg gactctatca
    19021 tgtcctgcat ggcctcattt ctcctatgaa tggtatcagt ccggacgata tcaatcttaa
    19081 gagccttatg actcgtctta tggatagtga ggtttcagaa gtgattgtgg cgactaatgc
    19141 tacagcggat ggtgaagcga cctccatgta tctttcacgt ttgctcaagc cggctggtat
    19201 caaggttacg cgtctagcac gaggtctcgc tgtgggagcg gacattgagt atgcggacga
    19261 agtgacactc ttacgagcga ttgaaaatcg gacagagttg taagtgtagg caaatttacg
    19321 aactccattc atttataaaa aatcaaagag gctgaaaatc gttcctatcg gcctcttttt
    19381 gtatagtgtg atgagtagaa tcaggttcaa gttttaaaaa accaagcaaa tatgatatac
    19441 taaagagcga gtattctagt agaattagga caaataatat gaaacaaacg attattcttt
    19501 tatatggtgg acggagtgcg gaacgcgaag tctctgtcct ttcagctgag agtgtcatgc
    19561 gtgcggtcaa ttacgaccgt ttcacagtca agactttctt tatcagtcag tcaggtgact
    19621 ctatcaaaac gcaggaattt agtcatgctc cggggcaaga agaccgtctc atgaccaatg
    19681 aaaccattga ttgggataag aaagttgcac caagtgctat ctacgaagaa ggtgcagtgg
    19741 tctttccagt ccttcacggg ccaatgggag aagatggctc tgttcaagga ttcttggaag
    19801 ttttgaaaat gccttacgtt ggttgcaaca ttttgtcatc aagtcttgcc atggataaaa
    19861 tcacgactaa gcgtgttctg gaatctgctg gtattgccca agttccttat gtggctatcg
    19921 ttgaaggcga tgatgtgact gctaaaatag ctgaagtgga agaaaaattg gcttatccag
    19981 tcttcactaa gccgtcaaac atggggtcta gtgtcggtat ttctaagtct gaaaaccaag
    20041 aagaactccg tcaagcctta aaacttgcct tccgatatga cagccgtgtc ttggttgagc
    20101 aaggagtgaa tgcccgtgaa attgaggttg gcctcttggg taactacgat gtcaagagca
    20161 cgctacctgg agaagttgtc aaggacgttg ccttttatga ctacgatgcc aagtatattg
    20221 ataacaaggt tactatggat attcctgcca aaatcagtga tgatgtggtg gctgtcatgc
    20281 gtcaaaatgc agaaacagcc ttccgtgcca ttggtggcct tggtctatct cgttgcgatt
    20341 tcttctatac agataaggga gagatttttc tcaacgagct caataccatg ccaggtttca
    20401 cccagtggtc tatgtaccca ctactttggg aaaatatggg gatcagctac ccagaactaa
    20461 tcgagcgttt ggttgacctt gccaaggaaa gttttgacaa gcgcgaagcg catttgatat
    20521 aaaatgaaag agagggtaga agccagaacc atcactgcaa ggtgactaga gttctcggac
    20581 ttcagccctt tttaaaggag tagaaatgaa attaacaatc catgaaattg cccaagttgt
    20641 tggagccaaa aatgatatca gtatctttga ggacactcag ttagaaaaag ctgagtttga
    20701 tagtcgtttg attggaactg gagatttatt tgtgccactt aaaggtgcgc gtgatggcca
    20761 tgactttatt gaaacagcct ttgaaaatgg tgcagcagta accttgtctg agaaagaggt
    20821 ctcaaatcat ccttacattt tagtagatga tgttttgaca gcctttcaat ccctagcatc
    20881 ctactatctt gaaaaaacga ctgttgatgt ctttgctgtt acaggttcaa atggcaagac
    20941 aacgactaag gatatgttgg cgcatttact gtcaacaaga tacaagacct acaaaacaca
    21001 aggcaattac aataatgaga ttggccttcc ttacacagtt cttcatatgc ctgaaggaac
    21061 agaaaagttg gttttggaga tgggacagga tcacttggga gatattcatc tcttgtctga
    21121 attggctcgt ccaaaaacag ccatcgtgac cttggttgga gaagctcatt tggccttttt
    21181 caaagatcgt tctgagattg ctaaaggaaa aatgcaaatt gcagacggaa tggcttcagg
    21241 ttccttgctt ttagcgccgg ctgaccctat cgtagaggac tatttgccaa ttgataaaaa
    21301 ggtggttcgt tttggtcaag gagcagagct ggaaattact gacttggttg agcgtaagga
    21361 cagtctgacc tttaaggcta atttcttgga acaagccctt gatttgccag taactggcaa
    21421 gtacaatgcg acaaatgcta tgattgcatc ctatgttgcc ttgcaagaag gagtttcaga
    21481 ggagcaaatt cgtttggcat tccaacatct tgaattgacg cgtaaccgta ccgagtggaa
    21541 gaaagcagcc aatggagcag atatcctgtc agatgtttac aatgccaatc caactgctat
    21601 gaaactgatt ttagagactt tctctgccat tccagccaat gaaggtggca agaaaattgc
    21661 agtgttggcg gatatgaagg agcttggtga ccagtctgtt caacttcata atcagatgat
    21721 tttgagcctc tccccagatg tgcttgatat cgttattttc tatggagaag atattgctca
    21781 gttggctcaa ttggccagtc aaatgtttcc aatcggccac gtttactact tcaagaaaac
    21841 agaagaccag gatcaatttg aagacctagt caagcaggtt aaggaaagcc ttggagccta
    21901 tgaccaaatc ttgctaaaag gttctaactc tatgaatcta gccaaattgg tagaaagttt
    21961 agaaaatgaa tgcaagtgat tttgctaagt atcttcaaag aatgattgct cttacagata
    22021 ctggactaac ctttacaaag gatccgtttg accgtgagcg ctacgaagac ttgcgaagtc
    22081 tgttatctga aatgttgaat caggtatcag accttgatgc agacgaagtg gcagaactct
    22141 tgaaaccgac ctccgcttat gcgactcctt taatggacgt ccgtgcttgg atagttgagg
    22201 atgaaaaaat ttgtctggtt aggggacaag gagagaatga ttgggctttg ccaggtggct
    22261 ttggcgaggt cggttattca ccaactgaaa atattctcaa ggaaattgaa gaagaaaccg
    22321 gttttaaagc caaagttgaa cgactactag ctgtttttga taccaatcgt ttccaactac
    22381 agagcaaaca atatgcaaag tttgtttttg aatgtaagct tctcgatgga caatttcaag
    22441 aaaatcaaga aatcgctgac cttcaatttt ttgcaattga ccaactgccg aatttatctg
    22501 aaaaacgcat taccaaggag caaatagagc ttctttggca ggtttatcaa ggtcataggg
    22561 ggcaatatct tgactaagaa gatgattatc gtatttctaa atccattttt aacaactagc
    22621 atggtataat aatatgcagg aaaattttga attatgagga agactagatg aatttatggg
    22681 atattttctt tacgactcag gcaaccgagc cgcccaaatt tgaccttttt tggtatgtta
    22741 gcctatttac gctcttagcc ttaacctttt atacagccca tcgctatcgt gaaaataagg
    22801 tttaccaacg atttttccaa atcttgcaga ctgttcagtt aatccttctt tatggttggt
    22861 actgggtcaa tcatatgcca ctgtcagaaa gcctaccctt ttaccattgc cgtatggcta
    22921 tgtttgtggt actcttgctt cctggtcaat ccaaatataa acaatacttt gcattattgg
    22981 gaacatttgg gacattagca gcctttgttt atccagtgcc agatgcttac ccttttccac
    23041 atatcaccat tctatccttt atctttggtc atttagcact cttggggaac tctctagttt
    23101 atctattgag acagtataat gcgcgattgc tggatgtgaa gggaattttt ctcatgacct
    23161 ttgccctaaa tgccttgatt tttgtggtca atttggtgac aggtggcgat tacggatttt
    23221 tgacaaaacc gccattggtt ggggatcacg gtctagtagc taattattta cttgtttcaa
    23281 ttgtgctggt agctactatc agtttgacta agaaaatctt agaattcttt ttagctcaag
    23341 aagcagaaaa aatgattgca aaggaagctt aacacagagc tttctttttt gctcttagag
    23401 agtttttaca agcagcttat aaaataagaa tttctgaata gacaaactca aaaaatggct
    23461 gggaaattta ggaaaaaagc aagcacgatt aaattttttg tgttataata ttttgtgaat
    23521 agctatgcct atgtttagct atggaataat acgaagtgcg aaacttggaa gatagagagg
    23581 aagcgatgta atggctagag aaggcttttt tacaggtcta gatattggaa caagctctgt
    23641 caaggtgctt gtggccgagc agagaaatgg tgaattaaat gtaattggcg tgagtaatgc
    23701 caaaagtaaa ggtgtaaagg atggaattat tgttgatatt gatgcagcag caactgctat
    23761 caagtcagcc atttcccaag cggaagaaaa ggcaggcatt tcgattaaat cagtgaatgt
    23821 cggcttgcct ggtaatcttt tgcaggtaga accaactcag gggatgattc cagtaacatc
    23881 tgatactaag gaaattacgg atcaagatgt tgaaaatgtt gtcaaatcag ctttgacaaa
    23941 gagtatgaca cctgaccgtg aagtcattac ctttattcct gaagaattta ttgtggatgg
    24001 tttccaaggg attcgtgacc cacgtggcat gatgggggtt cgccttgaaa tgcgtggttt
    24061 gctttataca ggacctcgta ctatcttgca caatttgcgt aagacggttg agcgtgcagg
    24121 tgttcaggtt gaaaatgtta tcatttcacc actagcaatg gttcagtctg ttttgaacga
    24181 aggggaacgt gaatttggtg ctacagtgat tgatatgggg gcaggtcaaa cgactgtcgc
    24241 tacaatccgt aatcaagaac tccagttcac acatattctc caagaaggtg gagattatgt
    24301 aactaaagat atctccaagg ttttgaaaac ctctcgcaaa ttagcggaag gcttgaaact
    24361 gaattacggg gaagcctatc cgcctcttgc aagcaaagaa accttccaag tagaggttat
    24421 tggagaagta gaagcagtcg aagtgacgga agcctacttg tcagaaatta tttctgcacg
    24481 aatcaagcac atccttgaac aaatcaagca agaattagat agaaggcgtc tattggacct
    24541 ccctggtggt attgtcttaa tcggtgggaa tgccatttta ccaggtatgg ttgagcttgc
    24601 tcaggaagtc tttggcgtcc gtgtcaagct ttatgtccca aatcaagttg gtatccgtaa
    24661 tccagccttt gcgcatgtga ttagtttatc agaatttgcg ggtcaattaa cagaagttaa
    24721 tcttttggct cagggagcga taaaaggtga gaatgactta agtcatcagc caattagttt
    24781 tggtgggatg ctgcaaaaaa cagctcagtt tgtacaatca acgcctgttc aaccagctcc
    24841 tgctccagaa gtagagccgg tggcgcctac agaaccaatg gcggatttcc aacaagcttc
    24901 acaaaataaa ccgaaattag cagatcgttt ccgtggcttg atcggaagca tgtttgacga
    24961 ataaagagga aaaataaatt atgacatttt catttgatac agctgctgct caaggggcag
    25021 tgattaaagt aattggtgtc ggtggaggtg gtggcaatgc catcaaccgt atggtcgacg
    25081 aaggtgttac aggcgtagaa tttatcgcag caaacacaga tgtacaagca ttgagtagta
    25141 caaaagctga gactgttatt cagttgggac ctaaattgac tcgtggtttg ggtgcaggag
    25201 gtcaacctga ggttggtcgt aaagccgctg aagaaagcga agaaacactg acggaagcta
    25261 ttagtggtgc cgatatggtc ttcatcactg ctggtatggg aggaggctct ggaactggag
    25321 ctgctcctgt tattgctcgt atcgccaaag atttaggtgc gcttacagtt ggtgttgtaa
    25381 cacgtccctt tggttttgaa ggaagtaagc gtggacaatt tgctgtagaa ggaatcaatc
    25441 aacttcgtga gcatgtagac actctattga ttatctcaaa caacaatttg cttgaaattg
    25501 ttgataagaa aacaccgctt ttggaggctc ttagcgaagc ggataacgtt cttcgtcaag
    25561 gtgttcaagg gattaccgat ttgattacca atccaggatt gattaacctt gactttgccg
    25621 atgtgaaaac ggtaatggca aacaaaggga atgctcttat gggtattggt atcggtagtg
    25681 gagaagaacg tgtggtagaa gcggcacgta aggcaatcta ttcaccactt cttgaaacaa
    25741 ctattgacgg tgctgaggat gttatcgtca acgttactgg tggtcttgac ttaaccttga
    25801 ttgaggcaga agaggcttca caaattgtga accaggcagc aggtcaagga gtgaacatct
    25861 ggctcggtac ttcaattgat gaaagtatgc gtgatgaaat tcgtgtaaca gttgttgcaa
    25921 cgggtgttcg tcaagaccgc gtagaaaagg ttgtggctcc acaagctaga actgctacta
    25981 actaccgtga gacagtgaaa ccagctcatt cacatggctt tgatcgtcat tttgatatgg
    26041 cagaaacagt tgaattgcca aaacaaaatc cacgtcgttt ggaaccaact caggcatctg
    26101 cttttggtga ttgggatctt cgccgtgaat cgattgttcg tacaacagat tcagtcgttt
    26161 ctccagtcga gcgctttgaa gccccaattt cacaagatga agatgaattg gatacacctc
    26221 catttttcaa aaatcgttaa gtaaatgaat gtaaaagaaa atacagaact tgtttttcga
    26281 gaagttgcag aggctagtct gagtgctaat cgagagagtg gttcggtctc tgtcattgca
    26341 gttaccaagt atgtagatgt accgacagcg gaagccttgc ttccgctagg tgttcatcat
    26401 atcggtgaaa atcgtgtaga taagtttctg gaaaaatatg aagctttaaa agatcgagat
    26461 gtgacttggc atttgattgg taccttgcaa agacgtaagg tgaaagatgt cattcaatac
    26521 gttgattatt tccatgcatt ggactcagta aagctagcag gggaaattca aaaaagaagt
    26581 gaccgagtca tcaagtgttt ccttcaagta aatatttcta aagaagaaag caaacacggt
    26641 ttttcgagag aggaactgct ggaaatcttg ccagagttag ccagactaga taagattgaa
    26701 tatgttggtt taatgacgat ggcacctttt gaggctagca gtgagcagtt gaaagagatt
    26761 ttcaaggcga cccaagattt acaaagagaa attcaagaga aacaaattcc aaatatgcct
    26821 atgaccgagt taagtatggg aatgagtcgt gattataaag aagcgattca attcggttcc
    26881 acttttgttc gtataggtac atcatttttt aagtaggaga gaaccatgtc tttaaaagat
    26941 agattcgata gatttataga ttattttacg gaggatgagg attcaagtct cccttatgaa
    27001 aaaagagatg agcctgtgtt tactccagta aattcttcac aggaaccggc tctcccaatg
    27061 aatcaacctt cacagtcggc tggtacaaaa gagaacaata tcaccagact tcatgcaaga
    27121 caacaggaat tggcaaatca gagtcagcgt gcaacggata aggtcattat agatgttcgt
    27181 tatcctagaa aatatgagga tgcaacagaa attgttgatt tattggcagg aaacgaaagt
    27241 atcttgattg attttcagta tatgacagag gtgcaggctc gtcgttgttt ggactatttg
    27301 gatggagctt gtcatgtttt agctggaaat ttgaaaaagg tagcttctac catgtatttg
    27361 ttgacaccag tgaatgttat tgtaaatgtt gaagatatcc gtttaccaga tgaagatcaa
    27421 cagggtgagt tcggttttga tatgaagcga aatagagtac gataatgatt tttttaattc
    27481 gtatgattta taatgcagtg gatatttact ccctgatttt ggtagccttc gctgtcatgt
    27541 cttggtttcc aggtgcctac gaatccagtt taggtcgttg gattgtagcg ttggtgaaac
    27601 cagtacttgc tcccttgcaa cgcctgcctt tacagatagc gggtcttgat ttatctgttt
    27661 gggtcgcgat tgttttggtt cgatttttag gagaaaacct agtgcgtttt ctggcgatga
    27721 taggatgaat aaagggattt atcagcattt ctccatagaa gatcgtccat ttcttgacaa
    27781 gggaatggaa tggataaaga aggtagaaga tagctatgct ccttttttaa ctccttttat
    27841 caatcctcat caggagaagc tattaaagat tttggccaaa acctatggtc ttgcttgtag
    27901 cagtagtggg gaattcgtct cgagtgagta tgttcgagtt ttattatacc cagattattt
    27961 ccaaccagag ttttcagatt ttgaaatatc tctccaagaa attgtgtatt ccaataaatt
    28021 tgaacattta acgcatgcta agattttagg gacagtcatc aatcaattag ggattgaacg
    28081 gaaacttttt ggagatatcc tagtagatga agaacgggcg cagattatga ttaatcagca
    28141 gtttcttctt ctctttcaag atggactaaa gaaaattggt cgtatacctg tttcgctgga
    28201 ggaacgtcct ttcaccgaga aaatagataa gctagaacag tatcgagaac tgaatttatc
    28261 tgtgtctagt tttcgattag atgttctttt atcaaatgtt ttgaaactat ctaggaatca
    28321 agcaaaccag ttgattgaaa agaaacttgt ccaagtaaat tatcatgtgg tagacaaatc
    28381 agattacact gttcaagttg gagacttgat tagtgtgaga aaatttggtc gcttgagatt
    28441 atttcaagat aagggacaaa cgaaaaaaga gaagaaaaaa ataaccgtcc agttattatt
    28501 aagtaagtga ggaatagaat gccaattaca tcattagaaa taaaggacaa gacctttgga
    28561 actcgattca gaggttttga tccagaagaa gtcgatgaat ttttagatat tgtggttcgt
    28621 gattacgaag atcttgtgcg tgcgaatcat gataaaaatt tgcgtattaa gagtttagaa
    28681 gagcgtttgt cttactttga tgaaataaaa gattcattga gccagtctgt attgattgcc
    28741 caggatacag ctgagagagt gaaacaggcg gcgcatgaac gttcaaacaa tatcattcat
    28801 caagcagagc aagatgcgca acgcttgttg gaagaagcta aatataaggc aaacgagatt
    28861 cttcgtcaag caactgataa tgctaagaaa gtcgctgttg aaacagaaga attgaagaac
    28921 aagagccgtg tcttccacca acgtctcaaa tctacaattg agagtcagtt ggctattgtt
    28981 gaatcttcag attgggaaga tattctccgt ccaacagcta cttatcttca aactagtgat
    29041 gaagccttta aagaagtggt tagcgaagta cttggagaac cgattccagc tccaattgaa
    29101 gaagaaccaa ttgatatgac acgtcagttc tctcaagcag aaatggaaga attacaagct
    29161 cgtattgagg tagccgataa agaattgtct gaatttgaag ctcagattaa acaggaagtg
    29221 gaaactccaa ctcctgtagt gagtcctcaa gttgaagaag agcctctgct catccagttg
    29281 gcccaatgta tgaagaacca gaagtagctc cagtgcatcc gacaggtcca acaccagcta
    29341 cagaaactgt tgattcagca ccaggatttg aagcaccgca agaatccgtt acaattttat
    29401 aagaaacgat gtgagaacaa tatcttatcc ttatatttcc agcgagcagg agatggtgtg
    29461 agtcctgcat tccctaacga taagattatc ctctcaaaaa ctcaagtctg aagctagtaa
    29521 gatttgacgt ttcccacgtt acgggataag agggagaaag actaaatctt tttccgaata
    29581 aaggtggtac cacgattttc gtcctttttg gaagtcgtgg tttttaattt gttattattt
    29641 ataaaggaga taccatgaaa ctcaaagata cccttaatct tgggaaaaca gctttcccaa
    29701 tgcgtgcagg ccttccgacc aaagagccag tttggcaaaa ggaatgggaa gatgcaaaac
    29761 tttaccaacg tcgtcaagaa ctaaatgaag ggaaaccgca ttttgtcttg catgatggcc
    29821 ctccatacgc aaacggaaat atccacgttg gacatgccat gaatcatatc tcaaaagata
    29881 tcatcattcg ttctaagtct atgtcaggtt ttaacgcacc ttatatccca ggttgggata
    29941 ctcatggtct gccaatcgag caagttttgg caaagcaagg tgtgaagcgc aaagaaatgg
    30001 acttggttga gtacttgaaa ctctgtcgtg agtacgctct ttctcaagtt tataaacaac
    30061 gtgatgattt caaacgcttg gggatgtcag gtgactggga aaatttatat gtgactttaa
    30121 ctccagacta tgaagcagcc caaatccgtg tctttggtga gatggctaat aagggttata
    30181 tctaccgtgg tgccaagcca gtttactggt catggtcatc tgagtcagcc cttgccgaag
    30241 cggaaattga ataccatgat ttggtttcga cttcacttta ttatgccaat aaggtaaaag
    30301 atggcaaagg tatcctagat acagatactt atatcgtagt ctggacaaca actccattta
    30361 ccatcacagc ttctcgtggt ttgactgttg gtgcagatat tgattacgtt ttggttcaac
    30421 ctgctagtga aactcgtaag tttgtcgttg ctgcagaatt gttgactagc ttgtctgaga
    30481 aatttggctg ggctgatgtt caagtgttgg caacttaccg tggtcaagaa ctcaaccaca
    30541 tcgtaacaga acacccatgg gatacagctg tagatgagtt ggtaatcctt ggtgatcacg
    30601 ttaccactga ctctggtaca ggtatcgtcc atacagcccc tggttttggt gaggacgact
    30661 acaatgttgg tattgctaat ggtcttgaag tcgcagtgac tgttgacgaa cgcggtatca
    30721 tgatggcgaa tgctggtcct gagtttgaag gtcagttcta cgacaaggtt gtaccaactg
    30781 ttattgaaaa acttggtaac ctccttctag cccaagaaga aatctctcac tcatacccat
    30841 ttgactggcg tactaagaaa ccaatcatct ggcgtgcagt tccacaatgg tttgcctcag
    30901 tttcaaaatt ccgccaagaa atcttggatg ctattgataa agttaaattc catacagaat
    30961 ggggtaaagt acgtctatat aatatgattc gtgaccgtgg tgactgggtt atctctcgtc
    31021 aacgtgcttg gggtgttcca cttccaatct tctatgcaga agacggtaca gctatcatga
    31081 cagctgagac tattgaacat gtggcgcaac tctttgaagt acatggttca agcatttggt
    31141 gggaacgtga tgccaaagac ctcttaccag aaggatttac acatccaggt tctccaaatg
    31201 gggagttcaa gaaagaaaca gatattatgg atgtatggtt tgactcaggt tcatcatgga
    31261 atggtgtttt ggtaaatcgt ccaaatctga cttatccagc tgacctttac cttgaaggtt
    31321 ctgaccaata ccgtggttgg ttcaactcat cactcattac atctgttgcc aaccatggcg
    31381 tagcacctta caaacaaatc ttgtcacaag gttttaccct tgatggtaaa ggtgagaaga
    31441 tgtctaaatc acttggaaat accattgctc caagtgatgt tgaaaaacaa tttggtgcag
    31501 aaatcttgcg tctctgggta acaagtgttg actcaagcaa tgacgtgcgt atctctatgg
    31561 atattttgag ccaagtttct gaaacttacc gtaagattcg taacactctt cgtttcttga
    31621 ttgccaatac atctgacttt aacccagctc aagatgtagt cgcttacgat gaacttcgtt
    31681 cagttgataa gtacatgact attcgcttta accagcttgt caagaccatt cgtgatgcct
    31741 atgcggactt tgaattcttg acaatctaca aggccttggt gaactttatc aacgttgact
    31801 tgtcagcctt ctaccttgat tttgccaaag atgttgttta cattgaaggt gccaaatcat
    31861 tggaacgccg tcaaatgcag actgttttct atgacattct tgtcaaaatc accaaactct
    31921 tgacaccaat ccttcctcac actgcggaag aaatctggtc atatcttgag tttgaagctg
    31981 aagacttcgt tcaattgtca gaattgccag aagctcaaac ttttgctaat caagaagaag
    32041 tcttggatac atgggcagcc ttcatggact tccgtggaca agctcaaaaa gccttggaag
    32101 aagctcgtaa tgcaaaagtt atcgggaaat cgcttgaagc acacttgaca gtttatccaa
    32161 atgaagttgt gaaaactcta ctcgaagcag taaacagcaa tgtggctcaa cttttgatcg
    32221 tgtcagactt gaccatcgca gaagggccag ctccagaagc tgcccttagc ttcgaagatg
    32281 tagccttcac agttgaacgc gctgcaggtc aagtatgtga ccgttgccgt cgtatcgacc
    32341 caacaacagc agaacgcagc taccaggcag tcatctgtga ccactgtgca agcatcgtag
    32401 aagaaaactt tgcggaagca gtcgcagaag gatttgaaga gaaataaggt tgaaaagtct
    32461 aggcaaaatt caatttgaga agaaaagaca actaatttta tagtctatta aacgcattgt
    32521 atcacgtttt tgaatacctg atatgatgcg ttttttattt attttaaaaa tttgcgaggt
    32581 atgacttttt atactcaaca agaatcaaag agaaacttag caagctaaca gtagtaagat
    32641 aaaataggaa tttgatatt
//
LOCUS       DAVMHG010000006        31295 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMHG010000006 DAVMHG010000000
VERSION     DAVMHG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.006"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            155..811
                     /locus_tag="VYR08_000219"
     CDS             155..811
                     /locus_tag="VYR08_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3465809.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            919..2014
                     /gene="prfB"
                     /locus_tag="VYR08_000220"
     CDS             join(919..990,992..2014)
                     /gene="prfB"
                     /locus_tag="VYR08_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV3465810.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQGEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            2032..2724
                     /gene="ftsE"
                     /locus_tag="VYR08_000221"
     CDS             2032..2724
                     /gene="ftsE"
                     /locus_tag="VYR08_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV3465811.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            2717..3643
                     /locus_tag="VYR08_000222"
     CDS             2717..3643
                     /locus_tag="VYR08_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3465812.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            3929..6109
                     /locus_tag="VYR08_000223"
     CDS             3929..6109
                     /locus_tag="VYR08_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3465813.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            6161..6976
                     /locus_tag="VYR08_000224"
     CDS             6161..6976
                     /locus_tag="VYR08_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV3465814.1"
                     /translation="MKFLTLNTHSWMEKEAKEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVVLAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            7116..8459
                     /locus_tag="VYR08_000225"
     CDS             7116..8459
                     /locus_tag="VYR08_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3465815.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            8836..10026
                     /locus_tag="VYR08_000226"
     CDS             8836..10026
                     /locus_tag="VYR08_000226"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV3465816.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            10579..11514
                     /locus_tag="VYR08_000227"
     CDS             10579..11514
                     /locus_tag="VYR08_000227"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV3465817.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            11548..12585
                     /locus_tag="VYR08_000228"
     CDS             11548..12585
                     /locus_tag="VYR08_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3465818.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            12758..13363
                     /locus_tag="VYR08_000229"
     CDS             12758..13363
                     /locus_tag="VYR08_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV3465819.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            13520..14050
                     /locus_tag="VYR08_000230"
     CDS             13520..14050
                     /locus_tag="VYR08_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV3465820.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            14071..15156
                     /gene="rlmN"
                     /locus_tag="VYR08_000231"
     CDS             14071..15156
                     /gene="rlmN"
                     /locus_tag="VYR08_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV3465821.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQINLAVS
                     LHALNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKAVAAVNP"
     gene            15156..15638
                     /locus_tag="VYR08_000232"
     CDS             15156..15638
                     /locus_tag="VYR08_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3465822.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            15640..17181
                     /locus_tag="VYR08_000233"
     CDS             15640..17181
                     /locus_tag="VYR08_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3465823.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(17237..18040)
                     /locus_tag="VYR08_000234"
     CDS             complement(17237..18040)
                     /locus_tag="VYR08_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV3465824.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(18124..18345)
                     /locus_tag="VYR08_000235"
     CDS             complement(18124..18345)
                     /locus_tag="VYR08_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465825.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEKVEDFQDDDEIPTII"
     gene            complement(18824..19024)
                     /locus_tag="VYR08_000236"
     CDS             complement(18824..19024)
                     /locus_tag="VYR08_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465826.1"
                     /translation="MNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMNLLAD
                     DSLAGDIILLWRIQFGTFTTET"
     gene            19203..19475
                     /gene="rpsP"
                     /locus_tag="VYR08_000237"
     CDS             19203..19475
                     /gene="rpsP"
                     /locus_tag="VYR08_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV3465827.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            19495..19734
                     /locus_tag="VYR08_000238"
     CDS             19495..19734
                     /locus_tag="VYR08_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV3465828.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            20311..21099
                     /locus_tag="VYR08_000239"
     CDS             20311..21099
                     /locus_tag="VYR08_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3465829.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            21130..21648
                     /gene="rimM"
                     /locus_tag="VYR08_000240"
     CDS             21130..21648
                     /gene="rimM"
                     /locus_tag="VYR08_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV3465830.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            21638..22357
                     /gene="trmD"
                     /locus_tag="VYR08_000241"
     CDS             21638..22357
                     /gene="trmD"
                     /locus_tag="VYR08_000241"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV3465831.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            22369..22707
                     /locus_tag="VYR08_000242"
     CDS             22369..22707
                     /locus_tag="VYR08_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465832.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(22737..23057)
                     /locus_tag="VYR08_000243"
     CDS             complement(22737..23057)
                     /locus_tag="VYR08_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465833.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            23155..23370
                     /locus_tag="VYR08_000244"
     CDS             23155..23370
                     /locus_tag="VYR08_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV3465834.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(23439..23525)
                     /locus_tag="VYR08_000245"
                     /pseudo
     CDS             complement(23439..23525)
                     /locus_tag="VYR08_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(23576..24112)
                     /locus_tag="VYR08_000246"
     CDS             complement(23576..24112)
                     /locus_tag="VYR08_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV3465835.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(24309..25655)
                     /gene="gor"
                     /locus_tag="VYR08_000247"
     CDS             complement(24309..25655)
                     /gene="gor"
                     /locus_tag="VYR08_000247"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV3465836.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            25941..27140
                     /locus_tag="VYR08_000248"
     CDS             25941..27140
                     /locus_tag="VYR08_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV3465837.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKISEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            27124..27825
                     /locus_tag="VYR08_000249"
     CDS             27124..27825
                     /locus_tag="VYR08_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465838.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            27827..29086
                     /locus_tag="VYR08_000250"
     CDS             27827..29086
                     /locus_tag="VYR08_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3465839.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            29210..31207
                     /gene="metG"
                     /locus_tag="VYR08_000251"
     CDS             29210..31207
                     /gene="metG"
                     /locus_tag="VYR08_000251"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV3465840.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT         9593 a         5664 c         6850 g         9188 t
ORIGIN      
        1 aaaaatttct taccttcatt cacagagctc gatttcagag ctctttttgc tagcttattc
       61 atacttttct gaatttcgaa aaagaaatgt aagcgtttga tagatttaca aaaagattgt
      121 ataataggga taagaataga aaaggagaag tctcatggca gttaaagatt ttatgacccg
      181 caaggtagtt tatattagtc cagatacaac agtatctcat gcagcagatt tgatgagaga
      241 gcaaggtttg caccgtctgc ctgttatcga aaatgatcaa ttagttggtt tggtgactga
      301 gggaaccatt gcacaagcaa gtccatctaa agcaacaagt ctttctatct atgagatgaa
      361 ttatcttctg aataagacaa aagtaaaaga tgtcatgatt cgcgatgttg tcactgtctc
      421 aggctatgct agtctagaag atgcaactta tctgatgttg aaaaataaga ttagtattct
      481 ccctgtcgta gataaccatc aagtatacgg agttattact gaccgtgacg ttttccaagc
      541 ctttcttgaa attgcaggtt atggcgaaga agggattcgt gtacgctttg ttacagaaga
      601 tgaagttggt gttcttggaa aaattgtttc tttgattgta gaagaaaatt tgaatatctc
      661 ccatacagtc aatattccgc gtaaggatgg taaggtgatt atcgaagtgc aaatcgatgg
      721 atcaattgat ttaccagcct tgaaagaaaa atttgaagca aatggtattc aagtggaaga
      781 aatcgctcgc acttcagcaa aagtcttgta agaagggaag cccaaaggct tcttttttca
      841 tgaaaagggg attagagaaa aagatggaaa gaaatgataa aatatgctat aatgaaataa
      901 tgtaaaaaag gagtatttat ggacatttca gtaattcgtc agaaaattga cgcaaatcgt
      961 gaaaaattag cttctttcag ggggtctctt tgacctcgaa gggctagagg aagagattgc
     1021 catcttggaa aacaagatga cagaacctga tttttggaac gataatattg cggcccaaaa
     1081 aacgtcgcaa gaattgaatg aattaaaaaa cacttacaat accttccata agatggaaga
     1141 gttgcagggt gaagtcgaaa ttttattgga ttttttggct gaagacgagt cagtgcatga
     1201 tgaactggta gcgcagttag ccgaacttga taagataatg accagctacg agatgactct
     1261 actcttgtca gaaccttatg accacaacaa tgccatcttg gaaatccatc caggttctgg
     1321 tggtactgag gcgcaggact ggggtgatat gttgcttcgt atgtatactc gttatggtaa
     1381 tgctaaaggc tttaaagtgg aagtgttgga ttaccaagca ggtgatgagg ctggtattaa
     1441 gtcggtaact ttatcatttg aagggcctaa tgcctatggt ctcctcaagt cagaaatggg
     1501 tgttcaccgc ttagtgcgaa tctcaccatt tgactctgcc aaacgtcgcc atacctcttt
     1561 cacatctgta gaagtgatgc cagaattgga tgatactatt gaagtggaaa tccgtgaaga
     1621 tgatatcaag atggatacct tccgttcagg tggtgccggt ggacaaaacg tcaataaggt
     1681 ttcaacaggt gtacgtttaa cccacattcc aactggaatt gttgtccaat caacagtgga
     1741 tcgtacccag tatggaaata gagatcgtgc catgaagatg ttgcaggcta agctctatca
     1801 aatggagcaa gataagaagg ctgcggaggt agattctctc aaaggtgaga aaaaggagat
     1861 cacttgggga agccaaatcc gttcttatgt cttcacgcct tatactatgg taaaagatca
     1921 ccgaactagc tttgaggttg ctcaggtaga taaggttatg gatggggacc tagatggttt
     1981 tatcgatgct tatctcaagt ggcgaattag ctaagataga aaggaactca catgtcaatt
     2041 attgaaatga gagatgtcgt taaaaaatac gacaacggaa cgactgctct acgcggtgtt
     2101 tcggttagcg ttcaaccggg ggaatttgct tacatcgtag gaccttcagg agcagggaag
     2161 tcaactttta ttcgttctct gtatcgtgaa gtaaaaatcg ataaaggaag cctatcagtt
     2221 gctggtttta atctggttaa gatcaaaaag aaagatgtcc cgcttctacg tcgtagtgtt
     2281 ggggttgtct tccaggatta taaattgtta ccaaagaaaa ctgtctatga aaatattgct
     2341 tacgctatgg aagtaatcgg ggaaaatcgc cgtaatatca aaagacgagt gatggaagtt
     2401 ttggacttgg ttggattgaa gcataaggtt cgttctttcc caaatgaact ctcaggtggg
     2461 gagcaacagc ggattgcgat tgcgcgtgca attgtaaata atcccaaagt attgatagct
     2521 gatgagccaa caggaaatct ggatccggat aattcatggg aaattatgaa tctcttggaa
     2581 cggattaacc tacaaggaac aactattttg atggcgactc ataatagcca gattgtaaat
     2641 accttgcgcc accgtgtcat tgccattgaa aatggccgtg tcgttcgtga cgaatcaaaa
     2701 ggagagtatg gatacgatga ttagtagatt ttttcgccat ttatttgaag ccttaaaaag
     2761 tttgaaacga aatggttgga tgacagtagc tgctgtcagt tcagtcatga ttactttgac
     2821 cttggtggca atatttgcat ctgttatttt caatacagcg aaactagcta cagatattga
     2881 aaataatgtc cgtgtagtag tttatatccg aaaggatgtg gaagataata gtcagacaat
     2941 tgaaaaagaa ggtcaaactg ttacaaataa tgactaccac aaggtatatg attctttgaa
     3001 gaacatgtct acggttaaaa gtgttacctt ttcaagtaaa gaagaacaat atgaaaaatt
     3061 aaccgagata atgggagata actggaaaat ctttgaagga gatgccaatc ctctctatga
     3121 tgcctatatt gtagaggcaa acactccaaa tgatgtaaaa actatagccg aagatgctaa
     3181 aaaaattgaa ggtgtctctg aggttcaaga tggcggtgcc aatacagaaa gactcttcaa
     3241 gttagcttca tttatccgtg tttggggact agggattgct gctttgttaa tttttatcgc
     3301 agttttcttg atttcaaata ccattcgtat taccattatt tcccgcagtc gcgaaattca
     3361 aatcatgcgc ttggtcggag ctaaaaacag ttatatccgt ggaccgttct tgttagaagg
     3421 agcctttatc ggtttattgg gagctatcgc accatctgtt ttggtcttta ttgtttatca
     3481 aattgtttac caatctgtca acaaatcgtt ggtagggcaa aatctatcca tgattagtcc
     3541 agatttattt agtccgttga tgattgccct actatttgtg attggggttt tcattggttc
     3601 attgggatca ggaatatcca tgcgccgatt cttgaagatt taggtaaaat agctgctttt
     3661 atgaggagat tgtaaaatct ccttttttgc tacaagagtt tttgaaaaga gatgcgtaga
     3721 agaaaagagc ttccaaagaa gtcccccaga gaagacttct tgatggctaa tttgaacttg
     3781 aaatttggcg cactagaaat tttttagaaa aaagtgtaat ttttttataa aagcctttac
     3841 aaaaaaaatt aaagtggtat aattaaatca tagaaggtgc aaacgttttc gtaaaacgtt
     3901 tgcctaaata aaaataaagg agatttgaat gatgaaagat acattcaaaa atgtcttgtc
     3961 tttcgaattt tggcaaaaat tcggtaaggc tttgatggta gttatcgcgg ttatgccggc
     4021 tgctggtttg atgatttcaa tcggtaagtc tatcgtgatg attaacccaa cctttacacc
     4081 acttgtcatc acaggtggaa ttcttgagca aatcggttgg ggggttatcg gtaaccttca
     4141 cattttgttt gccctagcca ttggaggaag ctgggctaaa gaacgtgctg gtggtgcttt
     4201 cgccgctggt cttgccttca tcttgattaa ccgtatcact ggtacaattt ttggtgtatc
     4261 aggcgatatg ttgaaaaatc cagatgctat ggtaactact ttctttggtg gttcaatcaa
     4321 agttgctgat tactttatca gtgttcttga agctccagcc ttgaacatgg gggtattcgt
     4381 agggattatc tcaggttttg taggggcaac tgcttacaac aaatactaca acttccgtaa
     4441 acttcctgat gcactttcat tcttcaacgg gaaacgtttc gtaccatttg tagttattct
     4501 tcgttcagca atcgctgcaa ttctacttgc tgctttctgg ccagtagttc aaacaggtat
     4561 caataacttc ggtatctgga ttgccaactc acaagaaact gctccaattc ttgcaccatt
     4621 cttgtatggt actttggaac gtttgctctt gccatttggt cttcaccaca tgttgactat
     4681 cccaatgaac tacacagctc ttggtggtac ttatgacatt ttaactggtg cagctaaagg
     4741 tactcaagta ttcggtcaag acccactatg gcttgcatgg gtaacagacc ttgtaaacct
     4801 taaaggtact gatgctagtc aataccaaca cttgttagat acagtacatc cagctcgttt
     4861 caaagttgga caaatgatcg gttcattcgg tatcttgatg ggtgtgattg ttgctatcta
     4921 ccgtaatgtt gatgctgaca agaaacataa atacaaaggt atgatgattg caacagctct
     4981 tgcaacattc ttgacagggg ttactgaacc aatcgaatac atgttcatgt tcatcgcaac
     5041 acctatgtat cttgtttact cacttgttca aggtgctgcc ttcgctatgg ctgacgtcgt
     5101 aaacctacgt atgcactcat tcggttcaat cgagttcttg actcgtacac ctattgcaat
     5161 cagtgctggt attggtatgg atatcgttaa cttcgtttgg gtaactgttc tctttgctgt
     5221 aatcatgtac tttatcgcaa acttcatgat tcaaaaattc aactacgcaa ctccagggcg
     5281 caacggaaac tacgaaactg ctgaaggttc agaagaaacc agcagcgaag tgaaagttgc
     5341 agcaggctct caagctgtaa acattatcaa ccttcttggt ggacgtgtaa acatcgttga
     5401 tgttgacgca tgtatgactc gtcttcgtgt aactgttaaa gatgcagata aagtaggaaa
     5461 tgcagagcaa tggaaagcag aaggagctat gggtcttgtc atgaaagggc aaggggttca
     5521 agctatctac ggtccaaaag ctgacatatt gaaatctgat atccaagata tccttgattc
     5581 aggtgaaatc attcctgaaa ctcttccaag ccaaatgact gaagcacaac aaaacactgt
     5641 tcacttcaaa gatcttactg aggaagttta ttcagtagca gacggtcaag ttgttggttt
     5701 ggaacaagta aaggatccag tatttgctca aaaaatgatg ggtgatggat ttgcagtaga
     5761 acccgcaaat ggaaacattg tatctccagt ttcaggtact gtatcaagca tcttcccaac
     5821 aaaacatgct tttggtattg tgacggaagc aggtcttgaa gtattggttc acattggttt
     5881 ggacacagta agtcttgaag gtaaaccatt tacagttcat gttgctgaag gacaaaaagt
     5941 tgcagcagga gatctccttg tcacagctga cttggatgct atccgtgcag caggacgtga
     6001 aacttcaaca gtagttgtct tcacaaatgg tgatgcaatt aaatcagtta aattagaaaa
     6061 aacaggttct cttgcagcta aaacagcagt tgctaaagta gaattgtaat atacttgagg
     6121 ttggaagctg tattccaacc tcttattttg ggagaaaaga atgaaatttt taacactcaa
     6181 tactcacagt tggatggaga aagaagcaaa ggaaaaattc cagattttgc ttgaagatat
     6241 tcttgaaaag gactatgatt tgatttgttt ccaagaaatc aatcaggaga tgacctcgtc
     6301 agaggtggag gttaatgacc tttatcaagc tttgccagca gctgagccta ttcaccaaga
     6361 ccattatgtt agactcttgg ttgaaaagtt gttagagcaa gggaaaaatt actactggac
     6421 ctgggcttat aaccatatcg gctatgaccg ctaccatgaa ggtgtggcta tcttgtctaa
     6481 aacacctatt gaagccagag aaattttggt ttcagatgtg gatgatccaa cagactatca
     6541 tactcgccgt gttgtcctag ctgaaactgt agtcgatggc aaggagctag cagttgccag
     6601 tgttcatctc tcttggtggg ataaaggttt ccaagaagaa tgggcacgat ttgaggctgt
     6661 cttgaaaaaa ttgaacaagc cacttttact agctggagat tttaacaacc cagcaggtca
     6721 ggaaggttac caagctattt tagctagtcc attaggctta caagacgcat ttgaagttgc
     6781 tcaagagaaa agtggtagct atactgttcc gcctgaaatt gatggctgga aagggaacac
     6841 tgaacccctt cgaatcgatt atgtctttac taccaaagag ttagcggtgg aaaatttaca
     6901 tgtcgtattt gatggtaaca agagtccaca agtgagtgat cactatggct tgaatgctat
     6961 attaaactgg aaataataac tgaaaagagg ttggaactat aaaattctag ccttttctta
     7021 ctagagaagc tactggaaat agcctaaata agtgagacta ctgtaatgga ataaaatatg
     7081 gtataattga taagatagat agaatcgagg atgttatgtc atttacgaaa tttcaattta
     7141 aaaactatat tagagaagcc ttgaaggagt taaaatttac aactccaaca gaggtgcaag
     7201 ataagttgat tcctattgtt ttggcaggtc gtgacctagt aggagaatca aaaacaggtt
     7261 caggtaagac tcatactttc ttgttaccga ttttccagca attagatgaa gctagcgata
     7321 gtgtacaagc agtgattact gcaccgagtc gtgagttggc tactcaaatt taccaagtag
     7381 cgcgtcagat ttcagctcac tcagatgtcg aagttcgtgt ggttaattat gtgggtggta
     7441 cggataaggc tcgccagatt gagaaattgg caagcaatca gcctcatatt gttattggaa
     7501 caccaggccg tatctacgac ttggttaaat ctggtgattt agctattcat aaagccaaga
     7561 catttgttgt tgatgaagcg gatatgacct tggatatggg attcttggaa actgttgata
     7621 agattgctgg cagtcttcca aaagacttgc aattcatggt cttctcagcg actatcccac
     7681 aaaaactgca accattcttg aaaaaatact tatccaatcc tgttatggag aaaattaaga
     7741 ccaaaacggt tatttctgac accattgata attggttgat ttcgaccaag ggacatgata
     7801 agaatgctca aatttaccag ttgactcagt tgatgcagcc gtatttggca atgatttttg
     7861 ttaacactaa aacgcgtgct gatgaattgc attcatatct gactgctcaa ggcttgaagg
     7921 ttgcaaaaat ccatggcgat attgcccctc gtgaacgcaa gcgaatcatg aatcaggtgc
     7981 aaaatctgga ttttgagtat attgtcgcaa cagatttggc agcacgtggg attgacattg
     8041 aaggtgtcag ccatgtcatc aatgatgcca ttccgcaaga cttatctttt tttgttcacc
     8101 gtgttggtcg tactggacga aatggcctac caggtacagc tattaccctt tatcagccaa
     8161 gtgatgactc ggatatccgt gagttggaga aattgggaat caagtttagt cctaagatgg
     8221 tcaaagacgg ggaatttcaa gatacctatg accgtgatcg tcgtgccaac cgtgagaaaa
     8281 aacaagataa acttgatatc gaaatgattg gtttggttaa aaagaaaaag aaaaaagtca
     8341 aaccgggtta taagaagaaa attcaatggg cggttgatga aaagcgccgt aaaaccaagc
     8401 gtgctgaaaa tcgcgctcgc ggtcgtgcag agcgtaaagc taaacgtcaa acattttaat
     8461 agaaattgtt ggagtactga gctccaactt ttttatttat gagaacgaac tatctaaacc
     8521 gaaacactac attaaagact gcaaattgcg attaaaaatg gtataatgat aaagttatat
     8581 agtcccgata agatggtagg tatttattac gaagagtttt cctatcagta gtttgcaatt
     8641 cgcaaagaat gacggcttcg aggtcgctcg tctttctacg gttgccgcca tactgcggtc
     8701 accctatgtg ccttactagc ttcagaaata aaaaagtatc gtttttattt ctgaagctag
     8761 taactcttag ataatggaaa ttcctatcag tactttgtaa ctctataaca atatttttta
     8821 aggggggaca tttttatgtc agaacgtaaa ttattcacgt ctgaatctgt atctgagggg
     8881 catccggata agattgcaga ccaaatttca gatgcgattt tggatgctat tttagcaaag
     8941 gatccagagg cgcacgttgc tgctgaaaca gctgtatata ctggttctgt ccacgttttt
     9001 ggtgaaattt ctacaaatgc ctatgtggat attaaccgtg tggttcgtga taccattgca
     9061 gagattggtt ataccaatac agaatatgga ttttctgctg agacggtggg agtacaccca
     9121 tctttggtgg aacaatctcc tgacatcgct caaggtgtta acgaagcctt ggaggttcgt
     9181 ggaaatgctg atcaagatcc actggacttg attggagcag gtgaccaagg gctcatgttt
     9241 ggatttgcag tagatgaaac agaagagctc atgccattgc caattgcact cagtcataaa
     9301 ttggttcgtc gtctggcaga acttcgtaag tctggagaaa ttagctatct ccgtccagat
     9361 gcaaaatcac aagttacagt tgagtacgat gaaaatgacc gtccggtacg tgtagataca
     9421 gtcgttattt ctactcagca tgatccagag gccactaatg aacaaatcca tcaagatgtg
     9481 attaacaagg tcatcaaaga agttattcca tcttcttatc ttgatgataa gacaaaattc
     9541 tttatcaatc cgacaggtcg ttttgtaatc ggtggtcctc aaggggactc aggtttgact
     9601 ggtcgtaaga ttattgtaga tacttatggt ggctactctc gtcatggtgg tggtgccttc
     9661 tctggtaaag atgcgactaa ggtggatcgt tcagcctctt atgcggctcg ctatattgcc
     9721 aagaatatcg ttgcagcagg ccttgctaag aaggcagaag tgcagttggc ctatgctatc
     9781 ggtgttgcgc aacctgtttc tgttcgtatc gatactttcg gtacaggaac agtagctgaa
     9841 agtcaacttg aaaaagcggc tcgtcaaatc tttgaccttc gccctgcagg gattatccaa
     9901 atgctggacc tcaagcgtcc aatttaccgt caaacatcgg cttacggtca catgggacgt
     9961 acagatattg atcttccatg ggaacgtttg gataaggtag atgctttgaa agaagcagta
    10021 aaataagatt ttaagagggg aacgtcctct cttttttata gtttttaact atactgggat
    10081 actgttctga aaatccattt tgcgaaagta gagatttaca tgtatagtag attgaaacta
    10141 gaatagtaca cttcaacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
    10201 ttctcctgtt cttgtttcat tttactatat ttctttaaaa atgataaagg ttgagatttc
    10261 tcctcgtaat agataatcat ggggatattt caatccaaag ttttattcgt tatcacttga
    10321 ctattgcaag gttttctaga gcaacagagt catggaatgg actcatggtt gagatttctc
    10381 cttgttgctt ggacttcatt caaaagtctg ttacccaagc cttgttcaaa cttccaatac
    10441 actagctgtt tctatagcat gacttctgta ctagactttc ttttccaaat aaatagatag
    10501 aaccacagaa tctagtaaac ctagaattaa aattatggta taatattagc aataaaagaa
    10561 atctggagga ttagaatcat ggtatcaacg aaaacacaaa ttgctggttt tgagtttgac
    10621 aattgcttga tgaatgcagc aggtgtggct tgtatgacga tagaggagtt agaagaggtc
    10681 aaaaactcag cggcaggaac ctttgttact aagacagcga ccttggactt ccgtcagggg
    10741 aatcctgagc cacgctacca agatgttcca cttggttcca tcaactctat gggcttgcca
    10801 aataatggct tagactatta tttggattat cttttagatt tgcaggaaaa agagtcgaac
    10861 cgaactttct tcttatctct ggtcggcatg tctccagagg aaacccatac tattttgaaa
    10921 aaagtccaag agagtgattt tcgtggtctg actgagctaa atctttcctg tccaaatgtt
    10981 ccaggtaaac ctcagattgc ctatgatttt gagacaacag accggatttt ggcagaagtg
    11041 tttgcttact tcaccaaacc tcttggaatt aaattgccac cttattttga tattgttcac
    11101 tttgatcaag cggcagctat tttcaacaaa tatccgctca agtttgtcaa ctgcgttaac
    11161 tctatcggaa acggcctcta tatagaagac gaatctgtcg ttattcggcc taagaatggt
    11221 tttggtggaa ttggtggaga atacattaaa ccgactgctc tagccaatgt tcacgccttt
    11281 tatcaacgtt taaatcctca aatccaaatt atcggaacag gtggcgttct gactggtcga
    11341 gatgcctttg aacacatcct ctgtggagca agtatggtgc aggtgggaac gacccttcac
    11401 aaagaaggcg tcagtgcttt tgaccgcatt accaatgaac tgaaagcaat catggtggaa
    11461 aaaggctacg agagcttaga agatttccgt gggaaattgc gctatattga ctaaattaaa
    11521 tcaaaaaatc tgaagaaagg agagacgatg ctagccattg aagaaagtca gaagttgact
    11581 ttatcaaatt taccgagcct gagcctattt acagggacag atcagggtca gtttgaagtg
    11641 atgaagagtc aaatgttgaa acagattggg tatgattctg ctgacctcaa ctttgcctac
    11701 tttgatatga aagaagtagt ttacaaggat gtggaactgg agttggtcag ccttcctttc
    11761 tttgcggatg agaaaatcgt gatattagat tattttatgg atatcacgac tgctaagaaa
    11821 cgctttttga cagatgatga gcttaagtca tttgaggaat accttgacaa tccttctcca
    11881 acaaccaagt tgataatctt tgcagaagga aagctggata gcaaaagacg gttagtcaaa
    11941 ttacttaagc gtgatgccaa ggtcttcgat gcagtagaag taaaagaaca agaattgcgc
    12001 cagtatttcc aaaagtggag tcagaaacaa ggtctgcagt ttaccaatca ttcttttgaa
    12061 aatctcctca tcaagtcggg gtttcaattt agcgaaatcc agaaaaatct tctcttttta
    12121 cagtcctata aggcgaattc tgttattgag gaagaggata ttgttaacgc aattcccaag
    12181 accttgcagg acaatatttt tgatttaact cagtttattc tgactaaaaa gatggatcag
    12241 gcgcgcgatt tggtgagaga cttgaccttg caaggggaag atgaaatcaa actgattgca
    12301 gtcatgctgg gacaatttcg gacttttact caggtgaaga ttttggcgga gtctggccaa
    12361 acagaatcgc agattgcaag tagtttaggt agttatctgg gacgtaaccc aaatccttat
    12421 caaatcaagt ttgcattaag agattcgaga ggactttctt tgagctttct gaagcaagct
    12481 atttcctatt tgattgagac agactatcag attaagacag gtctttatga aaaaggtttc
    12541 ctttttgaaa aggcactctt acagattgct agtcaggtca attgacattt gttgaaacta
    12601 caacccgcgt caggattatc ttgtcgtggg tttcttgctg atttgcttat tttattagaa
    12661 agtatgtcag tgatttcctt atctataagc aaaaaacttg aaaaaagtga atgtttgcgt
    12721 tatcattttc atatagaaag aaaaagaggt attacagatg gctattatct taccagaact
    12781 tccatatgca tacgacgctt tggaaccata catcgatgcg gaaacaatgc atttgcacca
    12841 tgacaaacac catcaaactt atgtcaacaa tgccaatgca gctttagaaa aacaccctga
    12901 aatcggtgaa gaccttgaag ccttgcttgc tgatgtagaa tctatcccag ctgatatccg
    12961 tcaagcactt atcaacaatg gtggcggaca cttgaaccac gctcttttct gggaattgat
    13021 gactcccgag aaaacagctc cttcagcaga actggcagca gcaatcgatg caacatttgg
    13081 ttcatttgaa gaattccaag cagccttcac tgcagcagca acaactcgtt ttggttcagg
    13141 ttgggcatgg ttggttgtca acaaagaagg gaaacttgaa gtgacttcaa cagcaaacca
    13201 agacacacca atctcagaag gtaaaaaacc aatcttgggc ttggacgttt gggaacatgc
    13261 ttactacgtg aaataccgca acgtgcgtcc tgactacatc aaagctttct tttcagtaat
    13321 caactggaat aaagtagatg aattgtacgc agctgctaaa taatgatagt tggagggaag
    13381 aattgttctt ctctttttta ggttataaga ttctggtctg acaaaatcgt cagacttttt
    13441 tcatttttat gagaaacgtg ctaactgcta gaaattatgg tagaataaag tattaagtaa
    13501 tcgtggaaaa aggatatcta tgcgaaaaga aattgcacct gaattataca actataacaa
    13561 gtttcctggt ccggagttcc atttacacgg ggacaaggtc gaaacggaag ggatagcttt
    13621 ttccttggtt gaaaatatca aggatgcctt tgatgtgacg acttttaatc agcgtttttc
    13681 agaagtatta accaagtttg attatatcgt gggggactgg agcaacgaac agcttcgcct
    13741 acgaggtttt tacaaggatg accgaacaga agaaaaactt gaaaaaatca gtcgtttaca
    13801 agactacctt ttagagtatt gtagttatgg ttgtgcctat tttgtcttag aaaatgaagc
    13861 ccctaagcga gcatcatttg acaagaaaat gcgtaagacg gaagaagaaa caccttctag
    13921 aaaaggaaag aaaccgactc aaactaaacg aaagtcgaat gcagataaga aaaatagacg
    13981 tcgtcagaaa gaccagcatt ctcagaaaga ggacaaggaa caacgtcatt ttgtcattcg
    14041 tcagaagtga gtagagaata aggagaagaa atgaaaccct caattcatag tttggcacat
    14101 caaaccatgc aggaatgggt attggagcag ggagaaaaga aattccgtgc agatcaaatc
    14161 tgggaatggc tctaccgtaa acgtgtgcag tcatttgaag aaatgaccaa cctttccaag
    14221 gatttgattg ctaagctcaa tgaccagttt gtggtcaatc ccttgaaaca acgtattgtg
    14281 caagagtctg ctgatggtac agtcaaatat ctctttgaat tgcctgacgg tatgttaatt
    14341 gagactgtac tcatgtgtca acactatggt ttatctgtct gtgtgaccac tcaggtcggc
    14401 tgtaatatcg gttgtacctt ctgtgcctct ggtttgatta agaagcagcg tgacctcaat
    14461 aacggtgaaa tcgtagcgca aatcatgctg gttcagaaat actttgatga gcgtggtcag
    14521 gatgaacgcg tcaaccatat cgttgtcatg ggaatcggtg agccctttga taattacaac
    14581 aatgtcttga atttctttcg tactatcaat gatgataaag gaatggctat cggtgctcgt
    14641 cacatcacgg tttcaacctc aggtttggct cataaaattc gtgactttgc tgatgaaggg
    14701 gttcagatca atcttgccgt gtctcttcac gcactcaaca atgaacttcg ttcaagtatc
    14761 atgaagatta accgtgcctt tccgattgaa aaactctttg ctgctattga atactacatt
    14821 gagacaacaa accgtcgtgt aacctttgaa tatatcatgc ttaatgaagt caatgacggt
    14881 gtagaacaag ccttggaatt ggctgaattg ctcaagaaca tcaagaaatt gtcttatgta
    14941 aacttgattc cttataaccc agttagtgag catgaccaat atagccgtag tcccaaagag
    15001 cgcgtgttgg ctttctatga tacgcttaag aaaaaagggg gcaactgtgt ggttcgtcaa
    15061 gagcatggta cagatattga cgcagcttgt ggacaattgc gctttaacac aatgaaacgt
    15121 gaccgccaga aggcagtcgc agcggtaaat ccttaatgat gaagaagact tataatcata
    15181 ttttggtctg gggagtcatt ttctatagca tttgcattgt ctgtttttgc tttactcctc
    15241 aagaacaatc taccgtggga gtgggaactc caggtattca gcatcttgga cgcctggttt
    15301 ttcttttgac tcctttcaat tctctctgga aactgggcga agtgagtgac attggacaat
    15361 tatgttggat ttttttacaa aatatcctca atgtcttctt gttttttcct ctgattttcc
    15421 aactccttta tctatttcca aatttgcgga aaacaaaaaa ggtccttctt tttagttttc
    15481 ttgtgagtct tggaatcgag tgtacgcaat taatcttaga ctttttcttt gatttcaatc
    15541 gcgtctttga gattgatgat ttgtggacca acactttggg tggctatctg gcttggctcc
    15601 tttataaacg attacataaa aacaagataa ggaattaaaa tgagtatttt agaagttaaa
    15661 aatctgagtc acggttttgg tgaccgtgca atttttgaag atgtgtcctt ccgtctcctc
    15721 aagggagaac atatcggcct ggtcggtgcc aatggtgaag gaaaatcaac ctttatgagt
    15781 atcgtgactg gtaaaatgct gccagatgaa ggaaaggttg agtggtccaa atatgtgacg
    15841 gctggttact tggatcagca ctctgtcctt gctgaaagac agtcggtgcg tgatgttctc
    15901 cgtacggctt ttgatgagct tttcaaagct gaagctcgta tcaatgacct ctatatgaaa
    15961 atggctgaag acggcgcgga tgttgatgct ctcatggaag aagtaggaga acttcaagac
    16021 cgtctggaga gtcgtgattt ctataccttg gatgctaaga ttgacgaagt agcgcgtgct
    16081 cttggtgtta tggactttgg catggatacg gatgtaactt ctttgtcagg tgggcaaaga
    16141 accaaggtgc ttttggcaaa acttctcctt gaaaagcctg atattttgct gttggacgag
    16201 ccgaccaact acttggatgc tgagcatatt gattggctca agcgctatct ccaaaactat
    16261 gagaatgcct ttgttctcat ttcgcacgat attccattcc tcaatgacgt tatcaatatt
    16321 gtctatcatg tggaaaatca acagctgacg cgttactctg gtgactacta ccagttccaa
    16381 gaagtttatg ctatgaagaa atctcagcta gaggcagcct acgaacgcca gcagaaagag
    16441 attgcagacc tcaaggactt tgtggctcgt aataaagccc gtgttgcaac tcgtaatatg
    16501 gctatgtctc gtcaaaagaa attggataag atggatatta tcgaactcca aagtgagaaa
    16561 ccaaaaccat cctttgattt caaaccagct cgtacaccag ggcgctttat cttccaagcc
    16621 aagaacttgc aaattggtta cgaccgtcct cttactaagc ctttaaatct taccttcgaa
    16681 cgcaatcaaa aggttgcgat tattggtgct aatggtattg gaaaaacaac tctcttgaag
    16741 agtctcttgg gcattatctc gccaatcgct ggggaagtgg agcgtggaga ttatttagaa
    16801 cttggttatt ttgagcagga agtagaaggc ggtaatcgcc aaactcctct tgaagctgtc
    16861 tggaatgcct ttcctgccct taatcaagca gaagtccgtg cagcccttgc ccgttgtggt
    16921 ttgacaacca aacatattga aagccagatt caagtattat cagggggaga gcaagccaag
    16981 gttcgtttct gtctcttgat gaatcgtgaa aacaacgttt tagtgctgga cgagccgacc
    17041 aaccatttgg atgtggatgc aaaggatgag ctcaaacgcg ctctcaaaga atatagggga
    17101 tctatcctta tggtctgcca cgagccagac ttttatgaag gctggataga ccaaatatgg
    17161 gattttaata atttaactta agaatgagag taaaagaaat acagtaccga atgtggatac
    17221 tgtatttttt tggtttttaa gatttaaaat cgtagtcttt caccacttcg atgctgtcga
    17281 ttgtgatagc agtagttggc ttgtcttttt catctttttc ggccttagca atcttatcca
    17341 caacatccat accgtcaatc acttgaccaa agactgggtg tttgccatct agactagggt
    17401 ttccaccttc tttgtaggct tcgataattt tctgtggata cttgcttgta gggagtttag
    17461 aagaggtatc tgtagagttt tggttgatga agaactggct gccattggtg tttggttgac
    17521 cagtattagc catagcaaga gcaccgcgga tgttatacaa ataaggagta atctcgttct
    17581 tgaaaccagt tcctttgtct ttagtcttat ccttgtcatg ccagatggac tgaccacctg
    17641 taccgtcccc ttttggatct ccagtttgga ccataaagcc atcgatgaca cggtggaagg
    17701 taataccgtt atagtagcct tctttggcgt gagtgaggaa attttcaacc gctagaggag
    17761 cgagtttagg gaagagttta atgcgaatat caccttggct tgtgtggaga atcacttcgg
    17821 cttcatcttc agcaacttcc ttagatagtt gtgggaaatt ggcgttttcg tttgttaaag
    17881 catcgtttaa ctccttggca gattgggcag ctactttgga actttcttca gcagcaagac
    17941 tggaatcaac ataatcatca ccacgcagac tgcgttggac gctgctacac ccagctaggg
    18001 ctacagtaga cagtaaaaga agggttgcta gttttttcat tctgatcctc tcaaaaattc
    18061 atttataacc attgtaccct aaaaggaagt ggatttcaaa tattagtagt ttacagaact
    18121 ggcttagata atagttggga tttcatcatc atcttgaaaa tcttcaactt tttcaacttc
    18181 ttccccagta aatggtttct gaaaagaact tagtataaaa cgggcaattg gttcaacaat
    18241 taggaaattt agaggaagag ctgcaataaa gtttacgaac caggcgctta gataggtatt
    18301 gggtgtgata gcaatgccat tgtaaaaaag tccatagaag gacatgaaag tgaccagcta
    18361 attcttcttc tgaaaaatgc tcatgaaggg cttgatccag gtcgcaagat ttcattttag
    18421 aaagtccagt aactcccttc ttcttcagga tattcttttt catagtagta tttagatggt
    18481 caagtgagtc aaaagaagtt tcgacaagag catacccttt ttctaccaga gtttttagat
    18541 gtttaggggc atcaatgcca taagtgtact caaagtattt agggaaccag atcttaagaa
    18601 agctcgtaaa gctagccagt tctctaaacg ttaagaacta gctacaatac agcatttaca
    18661 acacttcatg gttcacacca tggggtgttt tttaatgatt ttaagcaaaa ttcacactta
    18721 attgaaagtt atttaaatta gatgaaaatt tattttttga aaaagtggga aaacattgat
    18781 tctaagcgaa ttttgaatca tattcgaatg ggagttttta aaatcatgtt tcagtggtaa
    18841 aagtaccaaa ctggattctc catagtaaaa tgatatctcc tgctagtgaa tcatctgcta
    18901 ataagttcat atttcgcttg ggaacgggct tgggacttag gagccaagta tccaaatctc
    18961 gttcaggcga tatgaagggt ttgacttggt gttctttata aaatttttct aaaatagtat
    19021 tcacggatag tatctttcaa acatttattt cctattctaa caaatttcta aatactttaa
    19081 gaaatttata gtctgtcaag tttttttctt gacacatgcc agaaatctgc tataatagaa
    19141 catgtgctaa atagctcagc tatttcaccg aaataaaaaa ttaaagaaat gagactttaa
    19201 aaatggcagt taaaatccgt ttgactcgta tgggttctaa gaaaaaacct ttctaccgta
    19261 tcaacgtagc agattcacgt tcaccacgtg acggacgttt catcgaaaca gttggaactt
    19321 acaacccact tgttgctgaa aaccaagtaa ctttgaaaga agaccgcgtt cttgcatggt
    19381 tggctaatgg agctcaacct tcagacacag tacgtaacat cctttcaaaa gaaggcgtat
    19441 tgaaaaaatt ccacgattct aaattctcaa aataagttta aagtaggttg acagatggat
    19501 acgattgaaa atctcattat tgcgattgtg aaacccttaa tttcacaacc agatgcctta
    19561 actatcaaga ttgaagatac accagaattt ttggaatatc atttgaatct tgatcaaagc
    19621 gatgtgggtc gtgtaatcgg tcgtaagggt cgcactattt ctgcgataag aacgattgtc
    19681 tactctgtcc caactgaata caaaaaagta agaatcgtta ttgacgaaaa ataagaaggg
    19741 cgggacggat gtctcgcttt tttgtgtgga atagaagata tagtaaaatg aaacaagaac
    19801 aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gtaaaggtgt
    19861 actattctag tttcaatata ctataaaaga ttgaacgttt aggcaattcc aagactactt
    19921 actcgaacat taacagcagt ctcggactga tgaagttttc tttatgaagt tagtggagga
    19981 agtcggagaa gtagccgagg ttttgaatag gcactctggt cgaaaagaag ggataggatt
    20041 cgaacgagga acttgccaga gaactggctg atatcattta ctacaccgtc gcaatcgtag
    20101 ctattaatca tattgacctt accaagactg tctttgagaa agacaaaacc gcagccgtta
    20161 attaccaaca taaacataat ttggaaggat tattgaaggg aaagtagaga tattgtcccc
    20221 ttcctccaac ttgagaaaat ccttggaacg tgataaaata aaggataagg atttttaata
    20281 attcattata tagaatcagt ggatttcttt atgaagaggt ttgaagtatc tacagaaatt
    20341 ggtagcctct ctgttactta tcaaaagcaa aagaaagtgc tagtttgtct aaacggagca
    20401 ggtttgctac caagttatga aaatttttca cttatacttg aaaaacctcc tcctacaatt
    20461 ggttatttga caattgattt tccgaacaca ggtaggagtc cgattcatga ccaagctgga
    20521 aaaaatctag ataatcttgc agatgcggtt tatgaagtac ttgaagaatt agggatttct
    20581 gaatatatac tttgtgcaca tagttggagt ggaattttag cttgcaaatt actcgagaaa
    20641 ccaattaagc gtcagacttt agtagcaatt gaaccgacaa ctaaaaaagt catgtttgct
    20701 gatttttcag aaaatcctta tccagaaatg gaagagcaga tgaggctgat tgacgagtgt
    20761 ggtcctgaac tttattttaa gaacttaact caagcaacat ttagtcctga aacgaataaa
    20821 aaaatctggg aattaatgca agaaaaaggc ttagagttgg aaaatcaaga tccagaattt
    20881 cagatatctg gagagattac tgaggaagat tttgagaatt tgtcgataga atctcatgtc
    20941 cctgtattta ttttttgtca gacttataga gaaaaagagt acagagaatc agaatattgg
    21001 acttccaata ctaaactcat tttaggaggg aatcaccatt atttacaatg gtcagaatcg
    21061 gaaaaaattg cggctattat tcgagaattg tcagaataag atggaaaaaa ggagattaca
    21121 ggagacaaga tgaactactt taatgttggg aaaatcgtta atacgcaggg attacagggt
    21181 gagatgcgag tcttgtctgt gacggatttt gcagaagaac ggtttaaaaa aggagctgag
    21241 ctggctttgt ttgatgaaaa agatcagttt gtccaaacag tgaccatcgc tagccaccgt
    21301 aaacagaaga actttgacat tattaaattc aaagatatgt accatatcaa tactatcgaa
    21361 aagtacaagg gatacagtct caaggtcgct gaggaagatt tgaatgacct agacgatggt
    21421 gaattttact atcacgagat tatcggtttg gaagtctatg agggtgatag cttggttgga
    21481 accatcaagg aaatcctgca accaggtgct aatgatgtct gggtggtcaa acgaaaaggc
    21541 aaacgtgatt tgcttttacc ttatatccca ccagtggttc tcaatgttga tattccaaat
    21601 aaacgggtcg atgtggaaat cttagaaggg ttagacgatg aagattgata ttttaaccct
    21661 ctttccagag atgttttctc cactggagca ctcaatcgtt ggaaaggctc gagaaaaagg
    21721 gctcttggat atccagtatc ataattttcg agaaaatgct gaaaaggccc gtcatgtaga
    21781 tgatgagccc tacggaggcg gtcagggcat gttgctcaga gcacaaccta ttttcgattc
    21841 ctttgatgct attgaaaaga aaaatccgcg cgttattctc cttgatcctg ctggaaagca
    21901 gtttgatcag gcttatgctg aagatttggc tcaagaggaa gagctaatct ttatctgtgg
    21961 gcactatgag ggttatgatg agcgcattaa gaccttggta acagatgaga tttccctagg
    22021 tgactatgtc ctcactggtg gagaattggc agctatgacc atgattgatg ctacagttcg
    22081 cctgattcca gaagtgattg gcaaggagtc tagccaccaa gatgatagtt tttcttcagg
    22141 tcttttagaa tatcctcagt acacacgtcc ctatgattat cgaggcatgg tcgtgccaga
    22201 tgtattgatg agtggccatc atgaaaagat tcgtcagtgg cgattgtacg agagtttaaa
    22261 gaaaacctac gagcgcagac cggatttact tgaacattat caactgacag tagaagaaga
    22321 aaaaatgctg gcagaaatca aagaaaacaa agaataaagg agaaacctat gcaagtaatc
    22381 aaacgtaatg gcgaaattgc tgaatttaat ccagataaga tttaccaagc catcttgaag
    22441 gcagcccaaa ctgtttatgt tttgacagat gatttgcgtc aaaatcttgc tcaagtcact
    22501 aagaaggtag ttttggattt acaagaagcc aaggtggaac gtgcgactat cagtatgatt
    22561 caatctatgg ttgaacatcg tttattgggc gcaggttaca ttaccattgc agaacactat
    22621 atttcctatc gtttacaacg tgacttggaa agaagtggtt atggagatca tatcgcagtt
    22681 catttacatt ttgaacaaat tcgctaagaa aaagagtggg atgaagcaac tacatctcac
    22741 tctttcttaa atctattttt ttgggctgtt tggacagttg aggggacaaa tcgcaggcgt
    22801 tgaatatcgc ttatacggat aatccggctc acatttttgg aaaaattttt cacgataatt
    22861 tgctggcctt gctgatcata tttgatgatg tcacctgtaa agcttgtctc agataagata
    22921 aggtgaacag cggtttgttt ctggatagcc tcttcaatag tggctgtaag ggagttggtt
    22981 tcagtctggt caggttggtc atatccattt aaaaaatcat gtattttatt tagaacttgc
    23041 tggaatttat gtctcatatt ggcctccctt cttttatatc aatattatat aaaattttcc
    23101 taaaatctac aagattttac gaataaacta atgaaactaa aaaaggagaa gaaaatggca
    23161 gaatttacat ttgaaatcga agagcacttg ttgactcttt ctgaaaacga aaaaggttgg
    23221 accaaggaaa ttaaccgtgt gagctttaat ggtgcccctg caaagtttga tattcgtgct
    23281 tggagtccag accatactaa aatgggcaaa gggattactc tctcaaatga agaatttcaa
    23341 acgatggtgg atgcctttaa aggcaactaa tactcttcga aaatctcttc aaaccgcgtc
    23401 agctttgcct tgccgtatgt atggttactg acttcgtcag tttcatccac aacctcaaaa
    23461 cagtgttttg agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    23521 aagttttcaa aataaaagaa aaggatattg agtcatgata actcgatatc cttttttagt
    23581 tagtaaagta agcctgattt ttaaggcgtt gaagtagggg acggctaata aaactaatag
    23641 ccagaagttt gccaaggtct ggaatgataa agggaagaac ccccacagca agagcttttt
    23701 caaatgccat tccagctagg aaatgcaagc tgagaatccc gccgacaaag acaagggcat
    23761 cacccaagag gtttgcaaga aaaatcttaa caacaccact cttgctgttg gttagagagg
    23821 aagtaagtcc agagtaaacg agataaaacc aaagatagcc tgcagtaggg ccaactaaag
    23881 cctgaaaacc agctccacct cctgcaaaga caggaagacc gatagcacct agaagaagat
    23941 agagtccagc agaaagtaca gcctctctcg gtctaaagac agtagcaatc aagccgattg
    24001 caaagttttg cagagtgaag ggaacaggtc caattggaag actgatttgt gccaaagcag
    24061 caatgagagc agccccaata gcagggatag cataaacgtg agcttttttc aaaatagtta
    24121 acctcttttc taatgtatag taactattat actcatatag gagtcgatgt caaccatttt
    24181 ttaaaaacaa ggtaacaaaa actgtaacag ccgattttcg tgactagtca agttggataa
    24241 tttttttgtt gattcgaaaa tcattaaaaa agaagcagac atatttatat cagcttcttt
    24301 tgtttcgatt aacgcatggt tacaaattct tcagatgcag ttgggtgaat tgccacagtt
    24361 gcatcaaagt cagccttggt tgctcccatt ttgatagcaa cggcaaatcc ctgaatcatt
    24421 tcatcaacgc cgtagccaat tccatgaagt ccgacaactt tttcttctga accagctgtt
    24481 atgagtttga aacgggattc ttgacggttg cgagtgcaag cagagtacat agatgcaaag
    24541 cttgatttat aaaccttgat ttggtcttga ccgtattctt taatagcttg ctcttctgtc
    24601 aaaccaacag ttccgatagc agggtgtgaa aagacaacag ttggaatagt tgagtaatcc
    24661 atttttgcag tagtttttcc gttaaagaga cgttcagata gggtacgtcc ggccttgatt
    24721 gcaactggag tcagttcttt ctcgcccgtt acatcaccta gagcatagat tccctcaaca
    24781 acagtatttt ggtattcatc cacttggata aagccacgtt cgttcagagt cactccagct
    24841 ttttcaagtt gcaagccctt aacgtttgga cggcgacctg tagcccagat aacttggcta
    24901 gctgtgtgac tagtaccatc ttcgaaatga atggtaatgc cgtcagtagt tttttctaac
    24961 ttgacaggga ctttgtgagt gtgaagtggt aagtttgttc tttccatttc cttgaccaaa
    25021 ccttcaacga tgtaggaatc aaaaccacgt aaaggacgat cgcggcgaac aaagagatct
    25081 gtcttgacac caaaagtgtg gagtacgcca gctaattcaa cggcgatata accagcgcct
    25141 agaatggcaa ttgactctgg aagttcttcc caggcaaata catcatcaga agagccacct
    25201 agctcagcac caggaatatt aggaatactt ggatgggcac ctgtagcaat cacgatatgt
    25261 ttagcacgaa tcagttcacc atttacgctt acagtatgag aatctacaaa ttcagcatga
    25321 ccttcaatca agtctacacc gttgcgttta aaactaccat cataagaaga acgagcgcga
    25381 tcaatgtagg cttcacgatt gcgacgtagg gttgcaaagt taaagttaag atcagtagtc
    25441 ttaaagccgt agtcttctcc aaattgatgg aaagtctcag cgatttgcgc cccgtaccac
    25501 atgatttttt taggaacaca accgacgttg acacaggttc cacctaattt cttttcctca
    25561 ataacggctg ctttggctcc atgttcacca gcacggttca tggtagcgat tcctccgcta
    25621 cctccaccga tagcaatgat atcatattct ctcattgaaa actcctttgt aatcttttaa
    25681 atagtagagt gtcttttttg atagttatat tttattcttt ttttgatttg attgcaaaaa
    25741 tctgctacgc acaaaaaatt gcaaaaaagc ttgccaaata agtcaagata gtgtattata
    25801 tatctagtac aatagaaaag caaaattccg aacgattgtt taatcctgaa ctgccattat
    25861 ttccatctga actgcattgt ttgctggagg gaaatttggt ataatagcaa agtattgtaa
    25921 aaaagaaaag aagtttcatt atgaagaaaa agaatggtaa agctaaaaag tggcaactgt
    25981 atgcagcaat cggtgctgcg agtgtagttg tattgggtgc cggggggatt ttactcttta
    26041 gacaaccttc tcagactgct gtaaaagatg agcctactca tcttgttgtt gccaaggaag
    26101 gaagcgtggc atcctctgtt ttattgtcag ggacagtaac agcaaaaaat gaacaatatg
    26161 tttattttga tgctagtaag ggtgatttag atgaaatcct tgtttctgtg ggcgataaga
    26221 tcagcgaagg gcaggcttta gtcaagtaca gtagttcaga agcgcaggcg gcctatgatt
    26281 cagctagtcg agcagtagct aaggcagatc gtcatatcaa tgaactcaat caagcacgaa
    26341 atgaagccgc ttcagctccg gctccacagt taccagcgcc agtaggagga gaagatgcaa
    26401 cggtgcaaag cccaactcca gtggctggaa attctgttgc ttctattgac gctcaattgg
    26461 gtgatgcccg tgatgcgcgt gcagatgctg cggcgcaatt aagcaaggct caaagtcaat
    26521 tggatgcaac aactgttctc agtaccctag agggaactgt agtcgaagtc aatagcaatg
    26581 tttctaaatc tccaacaggg gcgagccaag ttatggttca tattgtcagc aatgaaaatt
    26641 tacaagtcaa gggagaattg tctgagtaca atctagccaa cctttctgta ggtcaagaag
    26701 taagctttac ttctaaagtg tatcctgata aaaaatggac tgggaaatta agctatattt
    26761 ctgactatcc taaaaacaat ggtgaagcag ctagtccagc agccgggaat aatacaggtt
    26821 ctaaataccc ttatactatt gatgtgacag gcgaggttgg tgatttgaaa cagggttttt
    26881 ctgtcaacat tgaggttaaa agcaaaacta aggctattct tgttcctgtt agcagtctag
    26941 taatggatga tagtaaaaat tatgtctgga ttgtggatga acaacaaaag gctaaaaaag
    27001 ttgaggtttc attgggaaat gctgacgcag aaaatcaaga aatcacttct ggtttaacga
    27061 atggtgctaa ggtcatcagt aatccaacat cttccttgga agaaggaaaa gaggtgaaga
    27121 ctgatgaagc aactaattag tctaaaaaat atcttcagaa gctaccgtaa tggtgaccaa
    27181 gaattgcagg ttctcaaaaa tatcaatcta gaagtgaatg agggtgaatt tgtagccatc
    27241 atgggaccat ctgggtctgg taagtccact ctgatgaata cgattggcat gttggataca
    27301 ccaaccagtg gagaatatta tcttgaaggt caagaagtgg ctgggcttgg tgaaaaacaa
    27361 ctagctaagg ttcgtaacca acaaatcggt tttgtctttc agcagttctt tcttctatcg
    27421 aagctcaatg ctctgcaaaa tgtagaattg cccttgattt acgcaggagt ttcgtcttca
    27481 aaacgtcgca agttggctga ggaatattta gacaaggttg aattgacaga acgtagtcac
    27541 catttacctt cagaattatc tggtggtcaa aagcaacgtg tagccattgc gcgtgccttg
    27601 gtaaacaatc cttctattat cctagcggat gaaccgacag gagccttgga taccaaaaca
    27661 ggtaaccaaa ttatgcaatt attggttgat ttgaataaag aaggaaaaac cattatcatg
    27721 gtaacgcatg agcctgagat tgctgcctat gccaaacgtc agattgtcat tcgggatggg
    27781 gtcatttcgt ctgacagtgc tcagttagga aaggaggaaa actaagatgc agaatctgaa
    27841 atttgccttt tcatctatca tggctcacaa gatgcgttct ttgcttacta tgattgggat
    27901 tattatcggt gtttcatcag ttgttgtgat tatggctttg ggtgattccc tatctcgtca
    27961 agtcaataaa gatatgacta aatctcagaa aaatattagc gtctttttct ctcctaaaaa
    28021 aagtaaagat gggtctttta ctcagaaaca atcagctttt acggtttctg gaaaggaaga
    28081 ggaagttcct gttgaaccgc caaaaccgca agaatcctgg gtccaagagg cagctaaact
    28141 gaagggagtg gatagttact atgtaaccaa ttcaacgaat gccatcttga cctatcaaga
    28201 taaaaaggtt gagaatgcta atttgacagg tggaaacaga acttacatgg acgctgttaa
    28261 gaatgaaatt attgcaggtc gtagtctgag agagcaagat ttcaaagagt ttgcaagtgt
    28321 cattttgcta gatgaggaat tgtccattag tttatttgaa tctcctcaag aggctattaa
    28381 caaggttgta gaagtcaatg gatttagtta ccgggtcatt ggggtttata ctagtccgga
    28441 ggctaaaagg tcaaaaatat atgggtttgg tggcttgcct attactacca atatctccct
    28501 tgctgcgaat tttaatatag atgaaatagc tagtattgtc tttcgagtga atgataccag
    28561 tttaacccca actctgggtc cagaactggc acgaaaaatg acagagcttg caggcttaca
    28621 acagggagaa taccaggtgg cagatgagtc cgttgtattt gcagaaattc aacaatcgtt
    28681 tagttttatg acgacgatta ttagttccat cgcagggatt tctctctttg ttggaggaac
    28741 tggtgtcatg aacatcatgc tggtttcggt gacagagcgc actcgtgaga ttggtcttcg
    28801 taaggctttg ggcgcaacac gtgccaatat tttaattcag tttttgattg aatccatgat
    28861 tttgaccttg ttaggtggct taattggctt gacaattgca agtggtttaa ctgccttagc
    28921 aggtttgtta ctgcaaggtt taatagaagg tatagaagtt ggagtatcaa tcccagttgc
    28981 cctatttagt cttgcagttt cggctagtgt tggtatgatt tttggagtct tgccagccaa
    29041 caaggcatcg aaacttgatc caattgaagc ccttcgttat gaatgagatc aacaagatgg
    29101 acactcgtct atcttgtttt tgtatggatt tccctatcga aattcattaa aaatgtgata
    29161 taatgaagtg ttagaaaaac aggtttcatt tgcctggaaa ggaaaaatta tgtctgaaaa
    29221 aaatttttat attacaacgc cgatttacta tccatctggg aaacttcata tcggttctgc
    29281 ctacacaact atcgcatgtg atgtcctagc acgttacaaa cgcctgatgg gctacgatgt
    29341 attttatctg acaggtcttg atgaacatgg tcagaaaatc cagcagaaag cggaagaagc
    29401 tggtattaca cctcaagcct atgttgatgg aatggcggtt ggagttaaag aactctggca
    29461 attactagat atctcatacg ataaatttat ccgtacaact gatgactacc atgaaaaagt
    29521 tgtcgcacaa gtctttgaac gcttacttgc tcaagatgat atctacttgg gtgaatactc
    29581 tggttggtat tcagtatcag acgaagaatt ctttacagaa agccagctgg cagaagtttt
    29641 ccgtgatgaa gctggaaatg tgactggtgg tattgctcca tcaggtcatg aggttgaatg
    29701 ggtttctgaa gaatcatact tccttcgcct tagcaaatac caagaccgtt tggtcgaatt
    29761 tttcaaagct catcctgaat ttatcacgcc agagggtcgc cttaatgaaa tgctacgcaa
    29821 cttcatcgag ccaggtttgg aagatttggc ggtatctcgt acaaccttta catggggagt
    29881 gcctgtccca tcaaatccaa aacacgttgt ctacgtttgg attgatgccc ttcttaacta
    29941 tgcgacagct cttggctacg ctcaagacga acatggtaac tttgacaagt tctggaatgg
    30001 aacagtcttc catatggtag gaaaagatat ccttcgcttc cactctatct actggccaat
    30061 ccttcttatg atgttggatg ttaaattacc tgatcgtttg attgcccatg gttggtttgt
    30121 catgaaagac ggaaaaatgt ctaagtcaaa agggaatgtc gtttaccctg aaatgttggt
    30181 agagcgttat ggactagatc cacttcgtta ctacctcatg cgtaaccttc cagttggttc
    30241 agacggaacc tttactcctg aagactatgt cggccgtatc aactatgaat tggctaatga
    30301 ccttgggaac ctccttaacc gtacggtttc catgattaat aagtactttg atggacaaat
    30361 ccctgcctat gtagaaggtg tgactgaatt tgatcatgtt cttgctgagg ttgcagaaca
    30421 atcaatcgca gacttccata cacacatgga agcagttgac tatccacgtg cgcttgaagc
    30481 agtctggact ctgatctctc gtaccaataa atacatcgat gagaccgcac catgggtctt
    30541 ggccaaggat gaagctcttc gtgaccaatt ggcaagtgtc atgagccact tggcagccag
    30601 cattcgtgta gttgctcact tgattgaacc atttatgatg gaaactagtc gtgcagtttt
    30661 gactcaactt ggtttggaag aagtttctag tcttgaaaac ttgagtttgg ctgacttccc
    30721 agcagatgtg actgtagttg ccaaaggaac acctatcttt ccacgtctaa atatggaaga
    30781 agaaatcgcc tatatcaagg aacaaatgga aggcaataaa ccagcagtcg aaaaagaatg
    30841 gaatccggac gaagttgagc tcaaactaaa caaggatgaa atcaagtttg aagactttga
    30901 caaggttgaa atccgtgtcg cagaagtcaa agaagtgtct aaagtagaag gttcagataa
    30961 gttgcttcaa ttccgcttgg atgctggtga tggagaagat cgtcagattc tttcaggaat
    31021 tgcaaaatac tatccaaatg aacaagaatt ggtcggcaag aaagttcaaa tcattgctaa
    31081 cctcaaacca cgtaaaatga tgaaaaaata tgtcagccag ggtatgattc tctcagctga
    31141 acatgatggc aaattaaccc ttctcacagt tgatccagct gtaccaaatg gaagtgtgat
    31201 tgggtaaaag tagacaggac tagttcaggc tagttctgtt tttttattta tactcaatga
    31261 aaatcaaaga gcaaactagg aagctagccg caggt
//
LOCUS       DAVMHG010000007        30200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMHG010000007 DAVMHG010000000
VERSION     DAVMHG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.007"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            54..488
                     /locus_tag="VYR08_000252"
     CDS             54..488
                     /locus_tag="VYR08_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3465841.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            500..1690
                     /locus_tag="VYR08_000253"
     CDS             500..1690
                     /locus_tag="VYR08_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV3465842.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1701..2192
                     /locus_tag="VYR08_000254"
     CDS             1701..2192
                     /locus_tag="VYR08_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3465843.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2207..2986
                     /locus_tag="VYR08_000255"
     CDS             2207..2986
                     /locus_tag="VYR08_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3465844.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2973..3791
                     /locus_tag="VYR08_000256"
     CDS             2973..3791
                     /locus_tag="VYR08_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3465845.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3791..4084
                     /locus_tag="VYR08_000257"
     CDS             3791..4084
                     /locus_tag="VYR08_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV3465846.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4106..6007
                     /locus_tag="VYR08_000258"
     CDS             4106..6007
                     /locus_tag="VYR08_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV3465847.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6067..7068
                     /locus_tag="VYR08_000259"
     CDS             6067..7068
                     /locus_tag="VYR08_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3465848.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7178..7513)
                     /locus_tag="VYR08_000260"
     CDS             complement(7178..7513)
                     /locus_tag="VYR08_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465849.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLTYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7524..7766)
                     /locus_tag="VYR08_000261"
     CDS             complement(7524..7766)
                     /locus_tag="VYR08_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465850.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7777..8319)
                     /locus_tag="VYR08_000262"
     CDS             complement(7777..8319)
                     /locus_tag="VYR08_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV3465851.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIRNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(8335..8529)
                     /locus_tag="VYR08_000263"
     CDS             complement(8335..8529)
                     /locus_tag="VYR08_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3465852.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8695..9645
                     /gene="rsmH"
                     /locus_tag="VYR08_000264"
     CDS             8695..9645
                     /gene="rsmH"
                     /locus_tag="VYR08_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV3465853.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9657..9974
                     /gene="ftsL"
                     /locus_tag="VYR08_000265"
     CDS             9657..9974
                     /gene="ftsL"
                     /locus_tag="VYR08_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV3465854.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9978..12230
                     /gene="pbpX"
                     /locus_tag="VYR08_000266"
     CDS             9978..12230
                     /gene="pbpX"
                     /locus_tag="VYR08_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV3465855.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSIFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12232..13212
                     /locus_tag="VYR08_000267"
     CDS             12232..13212
                     /locus_tag="VYR08_000267"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV3465856.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13821..15926
                     /locus_tag="VYR08_000268"
     CDS             13821..15926
                     /locus_tag="VYR08_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3465857.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            16378..16713
                     /locus_tag="VYR08_000269"
                     /pseudo
     CDS             16378..16713
                     /locus_tag="VYR08_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(16780..17262)
                     /locus_tag="VYR08_000270"
     CDS             complement(16780..17262)
                     /locus_tag="VYR08_000270"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV3465858.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(17357..18841)
                     /locus_tag="VYR08_000271"
     CDS             complement(17357..18841)
                     /locus_tag="VYR08_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV3465859.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            18994..20601
                     /locus_tag="VYR08_000272"
     CDS             18994..20601
                     /locus_tag="VYR08_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV3465860.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            20766..20933
                     /locus_tag="VYR08_000273"
     CDS             20766..20933
                     /locus_tag="VYR08_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV3465861.1"
                     /translation="MKKRILSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(20926..21447)
                     /locus_tag="VYR08_000274"
                     /pseudo
     CDS             complement(20926..21447)
                     /locus_tag="VYR08_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21670..23115
                     /locus_tag="VYR08_000275"
     CDS             21670..23115
                     /locus_tag="VYR08_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3465862.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            23117..23848
                     /gene="cps4B"
                     /locus_tag="VYR08_000276"
     CDS             23117..23848
                     /gene="cps4B"
                     /locus_tag="VYR08_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV3465863.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            23857..24549
                     /gene="cpsC"
                     /locus_tag="VYR08_000277"
     CDS             23857..24549
                     /gene="cpsC"
                     /locus_tag="VYR08_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV3465864.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            24559..25248
                     /locus_tag="VYR08_000278"
     CDS             24559..25248
                     /locus_tag="VYR08_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV3465865.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIITQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            25263..26624
                     /locus_tag="VYR08_000279"
     CDS             25263..26624
                     /locus_tag="VYR08_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3465866.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            26630..27373
                     /locus_tag="VYR08_000280"
     CDS             26630..27373
                     /locus_tag="VYR08_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV3465867.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            27370..28194
                     /locus_tag="VYR08_000281"
     CDS             27370..28194
                     /locus_tag="VYR08_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV3465868.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            28196..29074
                     /locus_tag="VYR08_000282"
     CDS             28196..29074
                     /locus_tag="VYR08_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3465869.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            29075..>30200
                     /locus_tag="VYR08_000283"
     CDS             29075..>30200
                     /locus_tag="VYR08_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV3465870.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFK"
BASE COUNT         9209 a         5259 c         6723 g         9009 t
ORIGIN      
        1 tacaatgggg aatataaagt aaaccggtaa acctaaacaa gaaaggagaa aagatgaaaa
       61 ttgtacttgt agggcatgga cattttgcta cagggattta tagttcttta caattgattg
      121 caggcaatca agaaaatgtg gaggcgattg actttgtgga aggaatgtca gcagatgaac
      181 tcaagcaaaa aatcttactt gcaatttcaa atgaagaaga agttttaatc ctaagtgatc
      241 tcttgggagg atcgccattc aaggtttctt ctaccataat gggagaaaat ccagccaaaa
      301 caatgaatgt tctctcgggt ttgaacttag ccatgttaat ggaagcagtc tttgctagaa
      361 tggctcatag ctttgatgag gttgttaata aatcagtagt ggcggcccag ggcggagtcg
      421 taaatggtaa agaattgttt tcaacggatg cagaggaaga ggaagaagat ttcgaatcgg
      481 gtatttaaag ggtaaaagaa tgataaaaaa ggttacgatt gaaaaaataa aatcgcctga
      541 gcgcttctta gaagtaccac ttctgacgaa agaagaagtc ggccaggcaa tcgataaggt
      601 tattcggcag ttagaactca accttgacta tttcaaggaa gatttcccta cgccagctac
      661 ctttgataat gtctatccaa tcatggataa cacggaatgg accaatggtt tctggacagg
      721 agaactgtgg ttggcttatg aatacagtca acaggatgca tttaaaaaca tcgctcataa
      781 aaatgttctt tctttcctgg atcgtgtcaa taagagagta gaattggatc accatgatct
      841 cggcttcttg tacacaccgt cttgtatggc tgaatataag ataaatggag atggagaggc
      901 tagagaagca accttgaaag ctgcagataa gttgattgaa cgctatcaag aaaaaggtgg
      961 ttttattcaa gcttggggag acttgggcaa gaaagagcat taccgtttga ttatcgactg
     1021 cttgctcaat atccaactct tattctttgc ttatcaagaa acaggcgatc aaaaatacta
     1081 cgatattgca gaaagtcatt tctatgcttc agctaataat gtaatccgtg atgacgcttc
     1141 gtccttccac accttctatt ttgatcctga gacaggtcaa ccctttaaag gtgtaacgag
     1201 acaagggtat agtgatgatt catgctgggc acgtggtcaa tcatggggag tctatggtat
     1261 tcctttgact tatcgtcact taaaagacga gtcctgtttt gacttgttta agggtgtgac
     1321 caattatttc ttgaatcgtc tgccaaaaga tcatgtgtcc tattgggatt tgatttttaa
     1381 tgatggtagt gatcaatcac gagattcttc agcaacagct atcgccgtct gtgggattca
     1441 tgaaatgcta aaacatctcc cagaggtgga tgctgacaaa gatatttata aacatgctat
     1501 gcatgccatg cttcgttcct tgatcgaaca ttatgcaaat gatcaattta cccctggtgg
     1561 gacaagtctc ctccacggtg tgtactcatg gcattcaggt aaaggagtgg atgaaggcaa
     1621 tatctggggt gactactatt acctagaagc ccttatccgt ttctacaaag actggaacct
     1681 atattggtag gaggagaaat atgacaatgc caaatattat tatgacccgt atcgatgaac
     1741 ggttgattca tggacaagga caactttggg taaaatacct aggttgtaat acggtcattg
     1801 ttgccaatga cgaagtaagc acggacaaga tgcaacaaac tctgatgaaa acagttgtgc
     1861 cagactcagt tgccatgcgt ttcttccctt tgcaaaaggt gattgatatc attcacaagg
     1921 ctaatcctgc tcaaacgatc tttatcgttg taaaggatgt gaaggacgct ttaaccttgg
     1981 tagaaggtgg tgtccctatc aaagaaatca atattgggaa cattcacaat gctactggta
     2041 aagagcaagt gacacgctcc atcttcctgg gtgaagagga caaggcagcc ctcaaggaat
     2101 taagccaaac tcatcaagta acatttaata cgaaaacaac tccaacagga aatgatggag
     2161 ctgttcaagt caacattatg gactatattt aaaagaggag atcgttatgt cgattaatgt
     2221 atttcaagcg attttaattg gattatggac agctttctgt tttagtggaa tgctattagg
     2281 aatttacacc aatagatgta ttgttctgtc atttggtgtc ggaattattc taggtgatct
     2341 gcctactgct cttgcaatgg gagctattgg tgaattggct tatatgggat tcggtgttgg
     2401 tgctggaggt actgttccac caaacccaat cggacctggt atctttggta ccttgatggc
     2461 tatcactagt gctggtaaag tcagtccaga agcggctctt gccctctcta ctccgattgc
     2521 tgtggcgatt caattcttac aaactttcgc ctacactgta cgtgctggtg cgcctgaaac
     2581 ggcgatgaag cacttgaaaa accataattt gaagaaattc aagttcactc taaatgcaac
     2641 aatttggttg tttgccttta ttggatttac cttgggttgc ttgggtgccc tttcaatgga
     2701 taccttgttg aaactcgtag actacattcc accggtattg cttacaggtt tgacagttgc
     2761 tggtaaaatg ctcccagcta tcggttttgc gatgatcttg tcagtgatgg ctaagaaaga
     2821 gttgattccc tttgtcttgt tgggatatgt ttgtgcagct tatctaaaca tcccaacaat
     2881 tggtattgca attgtaggta ctatctttgc tttgattgaa ttttataaca agccaaaaac
     2941 agcggatcat gtggtagagg aggaagcaca cgatgactgg atctaacaaa ttaacaaaac
     3001 gtgattatct taaaacgtct ttgcgggcat tcttttgtca aaatggattt aactatagta
     3061 actatcaagg gttgggatat gccaatgtga tgtatcctgc tttgaaaaaa cactatggag
     3121 agaatcagga aggtttctac caagccttgg aagaaaactg tgaattctat aataccaacc
     3181 cacacttcct gccttttatt accagcttgc atcttgtaat gttggaaaat ggccgtccgg
     3241 caaaagaaac acgtagcatc aagatggcct tgatgggacc attagcaggt attggggatt
     3301 ctctctctca attctgttta gctccattgt tctcaaccat cgcagcttcg tttgctcaag
     3361 aaggcttggt tgtcggtcca atcttgttct tccttgcgat gaatacgatt ttaacagcga
     3421 ttaaattgtc aactggtctg tatggataca aactaggaac gactgtgatt gataaactaa
     3481 gcgagcagat ggcaacgatt tctcgtattg ccaatattat tggtgtaacc gtaattgctg
     3541 gtttggcagc gacatctgtt aagattatgg taccgatcac ctttgctgca ggggaagtta
     3601 aagcagacgc taaacaaagt atcgtaagta ttcagggaat gcttgataag gttgctccag
     3661 ctcttctacc agccctattt acacttttag tttactactt gatcaaagaa aagaaatgga
     3721 caacatataa actcgttatt ttaacagtta tcatcggaat tatcggaagc tggcttaaga
     3781 ttatagctta atggatacaa cattgtttta tggaatagtg attgtcttgg cagtgagtcc
     3841 ccttttactg tcaagctttc attctattcg tcagcaaaag ttgcttcgca aacagatgga
     3901 gcaacgacaa gagtatttag cttctttaac atctggtgat gaagtgttgt tattgtctgg
     3961 aattcatgga aaaatcattt ctatccaaga tgacttggtc tccttgcaga ttgcaaaagg
     4021 tgtggtcatc tatgtagaaa aggaaagtgt aatgggaaag acaaaagaac tgctttttaa
     4081 gtagttcttt ttctggtaat agataatgaa agaagaaaga agacaatttt ttgaaagaat
     4141 cgatggaaac caatgtcgtg attatatctt gtcccactgt tcaaaagact atgagaaagt
     4201 caagagttcc cttgaacgct tgatggacaa tcgttttatg tttgatagtc cttgggatat
     4261 ggagccttgt tcaaaaatcc atcaaatcca gccgatggta tgggatcaag tatttgaaga
     4321 tgatccagaa tggtcttata tgctcaatcg acaagaatat ctcttgcagt ttatgatagg
     4381 gtatgtggta gaaggagata aggactatat tcaaaagtgc aagttctttc tatttgattg
     4441 gattgagcag gtgagagaat tttctcctca atccttgatg actagaacct tggataccgg
     4501 tattcgttcc tttacttggt tgaaactact cttgctcctc ttgaaatttg acttgctaga
     4561 ggagaaagaa ctagagaaaa ttttggtcag tctagaaaag cagattgact ttatgaaaag
     4621 ccactatcgc gccaagtaca cccttagtaa ctggggaatt ttacaaacaa ttccgatgct
     4681 tgctatctat catttctttg cagataagat ggacctagaa gaagcttacc attttgcttc
     4741 agaggagttg aaacagcaaa ttgagacaca gattttagga gatggaagcc agtttgaaca
     4801 gtcgattctc tatcatgtag aggtttataa agccttgctg gatttgtgtc tcttgcttcc
     4861 agacttgcaa gatagttacc aagagttgct ggaaaagatg gcgacctata ttcaaatgat
     4921 gacaggctta gatggacgaa ctttggcttt tggtgatagc gattctacag aaacgacaga
     4981 aatgttgagc ctgtctgctg tggttttgaa caaggaagac cttcttaacg gtctggatgt
     5041 taaagtcgat ttgcttagcc tcttgttcct ggggcgagaa aaggtcaagc gactgcagga
     5101 atttgaaaag agagcttggc agcctaagtc catgatcttt gaagactctg gacatgtctg
     5161 cattaaggat gaacatcgtt atctattttt caaaaatggt ccgctaggaa gtgcccatag
     5221 tcatagtgac gagaatagtt tttgcttaca gtatcaaggc caacctattt tcatagatgc
     5281 tgggcgttat tcttatcggg agatatatga acgttatctc ttaaagagtg cttggagtca
     5341 ttcgacctgc attgtagatg ggaaagctcc ggaaagaatc acgggatcct gggaatatga
     5401 atactatcct cactccctgt tttgtcacca taaagaaagg gagggagtgc attatattga
     5461 gggggcttat tggtcagcag aacctgattt gccttatctt cacaggagaa aaatcctcat
     5521 gttggtagag gatgtctggc tcttggtaga tgacatcagg tgtcaaggtc aacatgaggt
     5581 gttgactcag tttatccttg acaaggatgt gacctatcaa gatgggaaaa tcaatcagtt
     5641 gagactatgg agtgaagttg attttgattt ggaagatacc atcatttctc ctaaatacaa
     5701 tgagcttgaa agaagtagca aactcaccaa gcgccaattc tttgagaatc agatgctgga
     5761 ttataccatc attgcgcatg agagttttga aatcatccgt cattctgtct accagacaga
     5821 tgatcgtgaa gtggaaaatg ctctggcttt tgaagtgaaa aatgatgaaa cagacaagct
     5881 gattctgtta ttaagcgagg atattggtgt aggtgaaaaa ttgtgcctcg ttgacggaac
     5941 aaaaatgcgt ggaaaatgtt tagtatatga taaaataaat gagagaatga ttcgcttgca
     6001 gtgctagaaa taggcatttt gaatagtgaa tatgttataa taagtattag taggaggtgt
     6061 tttagattgg agaagaaact gaccataaaa gacattgcgg aaatggctca gacctcgaaa
     6121 acaaccgtgt cattttacct aaacgggaaa tatgaaaaaa tgtcccaaga gacacgtgaa
     6181 aagattgaaa aagttattca tgaaacaaat tacaaaccga gcattgttgc gcgtagctta
     6241 aactccaaac gaacaaaatt aatcggtgtt ttgattggtg atattaccaa cagtttctca
     6301 aaccaaattg ttaagggaat tgaggatatc gccagccaga atggctacca ggtaatgata
     6361 ggaaatagta attacagcca agagagtgag gaccggtata ttgaaagcat gcttctcttg
     6421 ggagtagacg gctttattat tcagccgacc tctaatttcc gaaaatattc tcgtatcatc
     6481 gatgagaaaa agaagaaaat ggtctttttt gatagtcagc tctatgaaca ccggactagc
     6541 tgggttaaaa ccaataacta tgatgccgtt tatgacatga cccagtcctg tatcgaaaaa
     6601 ggttatgaac attttctctt gattacagcg gatacgagtc gtttgagtac tcggattgag
     6661 cgggcaagtg gttttgtgga tgctttaaca gatgctaata tgcgtcacgc cagtctaacc
     6721 attgaagata agcatacgaa tttggaacaa attaaggaat ttttacaaaa agaaatcgat
     6781 cccgatgaaa aaactctggt atttatccct aactgttggg ccctacctct agtctttacc
     6841 gttatcaaag agttgaatta taacttgcca caagttgggt tgattggttt tgacaatacg
     6901 gagtggactt gcttttcttc tccaagtgtt tcgacgctgg ttcagccctc ctttgaggaa
     6961 ggacaacagg ctacaaagat tttgattgat cagattgaag gccgcaatca agaagaaagg
     7021 caacaagtct tggattgtag tgtgaattgg aaagagtcga ctttctaaaa tgaaggaaaa
     7081 tgacttgcaa tttctgttaa gaaataaaat aatcccacct agaacaagct aggtgggatt
     7141 atttgcctat gaaatgagaa attatgggag caagctccta aatcaactgt ttttgatcta
     7201 cttctttaac tacttgataa aagttataga agtaggtcaa acttgaaatg atggttacga
     7261 ctaggaatat tgaaaatttc cattggacag ggttggttaa aagttgtgga aaggatatga
     7321 ggagaaagaa gagggctgcg ttgaggacag gtatccgttt tgattgtatt ttctcaagtc
     7381 ctttattgag cgcaggaaga aagaggagta ggagtagtaa aactgtatga gaaacagctc
     7441 ctgaaataag ggcgaagaaa aggaaaatac tgataaaaac atgaatgatc agtagtctag
     7501 ctagtgattt cataaggcac ctcctaatcc tggtcttttt tagctcttgc aatacgaagt
     7561 gagtcgacaa tatgtatcat cactccgaaa aagaaagctc ccagtatagt tttaaaaata
     7621 tgttttgtat ttagaagaga actgataaaa tttggatttt cacttgttag ggtatcaatg
     7681 agtggaatta taaaaaatat cactgttcca taaatcgaac ctgctttcag acctggataa
     7741 cgtaactgtc tcttttcttt tttcataggg cacctcctaa tcctcatctt gatttttctt
     7801 agtttttgca atgcgacggg agatgaggaa ctgtatgctc gctccgaaga aaatagaacc
     7861 gagaatgctt gatacaccat ttcttatagt gagaagagaa tgaaaatagt cctgaccttc
     7921 acctatgagt atcctgagaa gaggagttat aaaaaacatc catagaccaa agaacaaacc
     7981 tgctttcaga cctgggtagt gtagttgctt gctttctttc tcattcagca tatctggttc
     8041 aatgactgtg atgcctgttt ttttcatttg gtaggtgaca tagccagcag cgatgagggc
     8101 aatcactaaa atcagaggag gatagattag agccacttct tgagggtatt tataggccag
     8161 aaggagtgga ataagatttc cgaaaatcat cagataaaag aggacgataa aaacttggtt
     8221 ccgaatacta tcggcctcac gccgtttgta ttcgtcaagg ggaccagaaa taccgtatgt
     8281 gcgtttgatc agtttttcag tgaaggtttc ttttttcata agtttgctcc ttttttaaaa
     8341 atcttcctcc caaaagagac tgttgaggtc agtttggagg ctgcgggcga gattgagaca
     8401 gagttccaag gttggattgt acttgtcgtt ttcaatcata ttgatagtct gtctcgagac
     8461 accgatatcc ttggcgagtt cgagctggga aatacccaat tccttgcgaa attctttcac
     8521 acgattcatc tgttctcctt tctgatttat gtcgtatata tttgactata ttatagtctt
     8581 ttaaacataa agtgtcaagt atttttgaca tattttttga agaaatagta gtctccttgt
     8641 cctatttgtc tgacaagtgc aagctggtcg gatttgtggt aaaatagata agatatgaca
     8701 aaagaatttc atcatgtaac ggtcttactc cacgaaacga ttgatatgct tgacgtaaag
     8761 cctgacggta tctacgttga tgcgactttg ggcggagcag gccatagcga gtatttatta
     8821 agtaaattaa gtgaaaaagg ccatctctat gcctttgacc aggatcagaa tgccattgac
     8881 aatgcgcaaa aacgcttggc accttacatt gagaagggaa tggtgacctt tatcaaggat
     8941 aacttccgtc atttacaggc acgtttgcgc gaagctggtg ttcaggaaat tgatggaatt
     9001 tgttatgact tgggagtgtc tagtcctcaa ttggaccagc gtgagcgtgg tttttcttat
     9061 aaaaaggatg cgccactgga catgcggatg aatcaggatg ctagtctgac agcctatgaa
     9121 gtggttaatc attatgacta tcatgacttg gttcgtattt tcttcaaata cggtgaggat
     9181 aaattctcta aacagattgc gcgtaagatt gagcaagcgc gtgaagtgaa gccgattgag
     9241 acaacgactg agttagcaga gattatcaag ttggtcaaac ctgccaagga actcaagaag
     9301 aagggtcatc ctgctaagca gattttccag gctattcgaa ttgaagtcaa tgatgaattg
     9361 ggggcggcag atgagtccat ccagcaggct atggatatgt tggctctgga tggtagaatt
     9421 tcagtgatta cctttcattc cttagaagac cgcttgacca agcaattgtt caaggaagct
     9481 tcaacagttg aagttccaaa aggcttgcct ttcatcccag atgatctcaa gcccaagatg
     9541 gaattggtgt cccgtaagcc aatcttgcca agtgcggaag agttagaagc caataaccgc
     9601 tcgcactcag ccaagttgcg cgtggccaga aaaattcaca agtaagaggg aaaaagatgg
     9661 cagaaaaaat ggaaaaaaca ggtcaaatac tacagatgca acttaaacgg ttttcgcgtg
     9721 tggaaaaagc tttttacttt tccattgctg taaccactct tattgtagcc attagtatta
     9781 tttttatgca gaccaagctc ttgcaagtgc agaatgattt gacaaaaatc aatgcgcaga
     9841 tagaggaaaa gaagaccgaa ttggacgatg ccaagcaaga ggtcaatgaa ctattacgtg
     9901 cagaacgttt gaaagaaatt gccaattcac acgatttgca attaaacaat gaaaatatta
     9961 gaatagcgga gtaagatatg aagtggacaa aaagagtaat ccgttatgcg accaaaaatc
    10021 ggaaatcgcc ggctgaaaac agacgcagag ttggaaaaag tctgagttta ttatctatct
    10081 ttgtttttgc cattttttta gtcaattttg cggtcattat tgggacaggc actcgctttg
    10141 gaacagattt agcgaaggaa gctaagaagg ttcatcaaac cacccgtaca gttcctgcca
    10201 aacgtgggac tatttatgac cgaaatggag tcccgattgc tgaggatgca acctcctata
    10261 atgtctatgc ggtcattgat gagaactata agtcagcaac gggtaagatt ctttacgtag
    10321 aaaaaacaca atttaacaag gttgcagagg tctttcataa gtatctggac atggaagaat
    10381 cctatgtaag agagcaactc tcgcaaccta atctcaagca agtttccttt ggagcaaagg
    10441 gaaatgggat tacctatgcc aatatgatgt ctatcaaaaa agaattggaa gctgcagagg
    10501 tcaaggggat tgattttaca accagtccca atcgtagtta cccaaacgga caatttgctt
    10561 ctagttttat cggtctagct cagctccatg aaaatgaaga tggaagcaag agcttgctgg
    10621 gaacctctgg aatggagagt tccttgaaca gtattcttgc agggacagac ggcattatta
    10681 cctatgaaaa ggatcgtctg ggtaatattg tacccggaac agaacaagtt tcccaacgaa
    10741 cgatggacgg taaggatgtt tatacaacca tttccagccc cctccagtcc tttatggaaa
    10801 cccagatgga tgcttttcaa gagaaggtaa aaggaaagta catgacagcg actttggtca
    10861 gtgctaaaac aggggaaatt ctggcaacaa cgcaacgacc gacctttgat gcagatacaa
    10921 aagaaggcat tacagaggac tttgtttggc gtgatatcct ttaccaaagt aactatgagc
    10981 caggttccac tatgaaagtg atgatgttgg ctgctgctat tgataataat acctttccag
    11041 gaggagaagt ctttaatagt agtgagttaa aaattgcaga tgccacgatt cgagattggg
    11101 acgttaatga aggattgact ggtggcagaa tgatgacttt ttctcaaggt tttgcacact
    11161 caagtaacgt tgggatgact ctccttgagc aaaagatggg agatgctacc tggcttgatt
    11221 atcttaatcg ttttaaattt ggagttccga cccgtttcgg tttgacggat gagtatgctg
    11281 gtcagcttcc tgcggataat attgtcaaca ttgcgcaaag ctcatttgga caagggattt
    11341 cagtgaccca gacgcaaatg attcgtgcct ttacagctat tgctaatgac ggtgtcatgc
    11401 tggagcctaa atttattagt gccatttatg atccaaatga tcaaactgct cggaaatctc
    11461 aaaaagaaat tgtgggaaat cctgtttcta aagatgcagc tagtctaact cggactaaca
    11521 tggttttggt agggacggat ccggtttatg gaaccatgta taaccacagc acaggcaagc
    11581 caactgtaac tgttcctggg caaaatgtag ccctcaagtc tggtacggct cagattgctg
    11641 acgagaaaaa tggtggttat ctagtcgggt taaccgacta tattttctcg gctgtatcga
    11701 tgagtccggc tgaaaatcct gattttatct tgtatgtgac ggtccaacaa cctgaacatt
    11761 attcaggtat tcagttggga gaatttgcca atcctatctt ggagcgggct tcagctatga
    11821 aagactctct caatcttcaa acaacagcta aggctttaga gcaagtaagt caacaaagtc
    11881 cttatcctat gcctagtgtc aaggatattt cacctggtga tttagcagaa gaattgcgtc
    11941 gcaatcttgt acaacccatc gttgtgggaa caggaacgaa gattaaaaac agttctgctg
    12001 aagaagggaa gaatcttgcc ccgaaccagc aagtccttat cttatctgat aaagcagagg
    12061 aggttccaga tatgtatggt tggacaaagg agactgctga gacccttgct aagtggctca
    12121 atatagaact tgaatttcaa ggttcgggct ctactgtgca gaagcaagat gttcgtgcta
    12181 acacagctat caaggacatt aaaaaaatta cattaacttt aggagactaa tatgtttatt
    12241 tccatcagtg ctggaattgt gacattttta ctaactttag tagaaattcc ggcctttatc
    12301 caattttata gaaaggcgca aattacaggc cagcagatgc atgaggatgt caaacagcat
    12361 caggcaaaag ctgggactcc tacaatggga ggtttggttt tcttgattac ttctgttttg
    12421 gttgctttct ttttcgccct atttagtagc caattcagca ataatgtggg aatgattttg
    12481 ttcatcttgg tcttgtatgg cttggtcgga tttttagatg actttctcaa ggtctttcgt
    12541 aaaatcaatg aggggcttaa tcctaagcaa aaattagctc ttcagcttct aggtggagtt
    12601 atcttctatc ttttctatga gcgcggtggc gatatcctgt ctgtctttgg ttatccagtt
    12661 catttgggat ttttctatat tttcttcgct cttttctggc tagtcggttt ttcaaacgca
    12721 gtaaacttga cagacggtgt tgacggttta gctagtattt ccgttgtgat tagtttgtct
    12781 gcctatggag ttattgccta tgtgcaaggt cagatggata ttcttctagt gattcttgcc
    12841 atgattggtg gtttgctcgg tttcttcatc tttaaccata agcctgccaa ggtctttatg
    12901 ggtgatgtgg gaagtttggc cctaggtggg atgctggcag ctatctctat ggctctccac
    12961 caagaatgga ctctcttgat tatcggaatt gtgtatgttt ttgaaacaac ttctgttatg
    13021 atgcaagtca gttatttcaa actgacaggt ggtaaacgta ttttccgtat gacgcctgta
    13081 catcaccatt ttgagcttgg gggattgtct ggtaaaggaa atccttggag cgagtggaag
    13141 gttgacttct tcttttgggg agtgggactt ctagcaagtc tcctgaccct cgcaatttta
    13201 tatttgatgt aagaatggca ccctgatgtt tcagggtgtt tttgtgttta aatacacaat
    13261 gaaaatcaaa gaacaaacta gaaagctaac tttaggctgc tcaaaatata gtagattgaa
    13321 actaaaatag tacacatcta cttctaaaat attgttagaa atcgatttga ctgtcctgat
    13381 tgatttgtcc tgttcttatt tcattttact atacagtttt gagatggttg atagaactga
    13441 cgaagtcagc tcaaaacacg gttttgaggt tgtagataag gagaagctga tgtggtttga
    13501 agaggttttc gaagagtgta aaaaaatatt ggtcagttaa agtcaaaact cttgaccttt
    13561 tctgactaat gtagtatatt atgaacgtaa ggtaaatgaa agccttacta gaacacttat
    13621 ttaaagtaaa atagaaagag gtgggtctat gtttcatcta gaaatcttca gaagtaaaga
    13681 tagtctactc ctgcttgaaa aagaaaaacc agaaatagta cgtagggtag cgatctagct
    13741 agtctaaatt ttttaaaaca atggtcaaag atagtcaata tcagtaatca taactaagta
    13801 aacaaaaaga ggtaaagaat atgaataaca actttaataa ttttaataac atggatgatt
    13861 tatttaacca attgatgggt ggtatgcgag gatacagttc tgaaaatcgc cgttacttaa
    13921 ttaatggacg cgaagtcaca cctgaggaat ttgctcacta tcgtgcgact ggtcaattac
    13981 caggaaatgc agaaactgat gtgcaaatgc cacaacaggc atcaggtatg aaacaagacg
    14041 gtgtccttgc aaaactaggt cgaaacttaa cagcagaagc gcgtgagggc aagttggatc
    14101 ctgttatcgg acgaaacaag gaaattcaag aaacatctga aatcctctca cgccgcacca
    14161 agaacaatcc tgttttggtc ggagatgcag gtgttggtaa gacagcagtt gtcgaaggtc
    14221 tagcgcaagc cattgtgaac ggagatgttc ctgctgctat caagaacaag gaaattattt
    14281 ctattgatat ctcaggtctt gaggctggta ctcaataccg tggtagcttt gaagaaaatg
    14341 tccaaaactt agtcaatgaa gtgaaagaag cagggaatat tatcctcttc tttgatgaaa
    14401 ttcaccaaat tcttggtgct ggtagcactg gtggagacag tggttctaaa ggacttgcgg
    14461 atattctcaa gccagctctc tctcgtggag aattgacagt gattggggca acaactcaag
    14521 acgaataccg taacaccatc ttgaagaatg ctgctcttgc tcgtcgtttc aacgaagtga
    14581 aggtcaatgc tccttcggca gagaatactt ttaaaattct tcaaggaatt cgtgacctct
    14641 atcaacaaca ccacaatgtc atcttgccag acgaagtctt gaaagcagcg gtggattatt
    14701 ctgttcaata cattcctcaa cgtagcttgc cagataaggc tattgacctt gtcgatgtaa
    14761 cggctgctca cttggcggct caacatccag taacagatgt gcatgctgtt gaacgagaaa
    14821 tcgaaacgga aaaagacaag caagaaaaag cagttgaagc agaagatttt gaagcagctc
    14881 taaactataa aacacgcatt gcagaattgg aaaagaaaat cgaaaaccac acagaagata
    14941 tgaaagtgac tgcaagtgtc aacgatgtgg ctgaatctgt ggaacgaatg acaggtatcc
    15001 cagtatcgca aatgggagct tcagatatcg aacgtttgaa agatatggct catcgcttgc
    15061 aagacaaggt gattggtcaa gataaggccg tagaagctgt agctcgtgct atccgtcgta
    15121 accgtgctgg ttttgatgaa ggaaatcgcc caatcggcag cttcctcttt gtagggtcta
    15181 ctggggttgg taagacggag cttgctaagc aattggcact cgatatgttt ggaacccagg
    15241 atgcgattat ccgtttggat atgtctgaat acagtgaccg cacagctgtt tctaagctaa
    15301 ttggtacaac agcaggctat gtgggttatg atgacaatag caatacctta acagaacgtg
    15361 ttcgtcgcaa tccatactct atcattctct tggatgaaat tgaaaaggct gatcctcaag
    15421 ttattaccct tctcctccaa gttctagatg atggtcgttt gacagatggt caaggaaata
    15481 cagtaaactt caagaatact gtcgttattg cgacctcaaa tgctggattt ggctatgaag
    15541 ccaatttgac agaagatgcg gataaaccag aattgatgga ccgtttgaaa cccttcttcc
    15601 gtccagaatt cctcaaccgc tttaatgcag tcatcgagtt ctcacacttg actaaggaag
    15661 acctttctaa gattgtagat ttgatgttgg ctgaagttaa ccaaaccttg gctaagaaag
    15721 acattgactt ggtagtcagt caagcggcta aagattatat cacagaagaa ggttacgacg
    15781 aagtcatggg ggttcgtcct ctccgtcgcg tggttgaaca agaaattcgt gataaggtga
    15841 cagacttcca cttggatcat ttagatgcta aacatctgga agcagatatg gaagatggcg
    15901 ttttggttat tcgtgagaaa gcctaagaca gagttttgag gataaaaaag aaggagccag
    15961 ctgaaaaaaa ctggttcctt ttgaggtacg acgggcatgt cgtacagtag gttgaaacta
    16021 gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactga cctgattgat
    16081 ttgtcctgtt cttatttcat tttactatat atctgaggtg taagtcctcg gtgggtacct
    16141 gctaccggtg ctcccaatag tgattcccaa gcctgactat cgtgaggtag cggattaaaa
    16201 cggtctgggg atagaccgtt ttaagtctga cgctggaaat aagaatcgtc agaggaaggg
    16261 atagcgaaat cgtggctcta cgaataggaa cgtgataata aggcgtatat agcggataag
    16321 gggcctcaaa ctctaaagtt caaaaaggta gtcgtagcct atatgcgtaa atcacgagag
    16381 caactgaatt tgtctgttcg aggatggata aactatttct cattgggaaa tatgaaaagt
    16441 atagtcacta gcatagatga gcgcttgcgt actcgcctac gagtgattat ctggaagcaa
    16501 tggaagaaga aatctagacg attatggaga ttgcttaagt taggggttcc taaatggata
    16561 gcagataagg tatctggctg gggtgaccac tatcaattag tagctcagaa gtcggtactt
    16621 aaacgtgcta tatcaaaacc agtccttgaa aaacgtggac tggtttcgtg tttggattat
    16681 taccttgaac gacatgcgtt aaaagttagt tgaacagccg tatgccgaac ggcacgtacg
    16741 gtggtgtgag aggggctaga gattatcccc tactcgattt taaatcacat gacgttcaaa
    16801 ggcatcatct gaaatccctt gttccaagat gagttttgcc cattctttag cagagaagag
    16861 gctgtggtcc ttgtagtttc cgcaagattc gatggttgtc cctgggacat cttcccaagt
    16921 agtagtttca gcgatttcct tgagcgaatc cttgataaca gctgcgattt tagcactggt
    16981 gtgacgtccc cacataatca tgtggaagcc tgtgcggcaa ccaaatggtg aacagtcaat
    17041 catgccgtca atgcgggtac ggatgagttt ggctaagagg tgctcgatag tgtgaaggcc
    17101 ggcagtaggg atagagtctt cgtttggttg caccaagcga atatcataat tggagatgat
    17161 gtctcctttt ggtcctgttt cttccccaat caagcgaaca tagggtgctt tgacaatggt
    17221 gtggtcaagt tcaaaacttt cgacaataac ttcttttgac atggtaaatc ctttcagttt
    17281 tcttctctca ttatatcata aaggttgctc ctgagacaga gagaaaacct ctccgaggct
    17341 ggagaggttg aaatctttac ttacgatata agcggtcgta ttggtagtat gggtcaaagg
    17401 ttacgttgat acccagttta cgaaggacat tcttgtcttc atcagtcaag atgatggttg
    17461 agtgggcttc gcttcctttg aggttgccga gttcttccat agcgcgggca gcatcaggat
    17521 tttctgtagc tgtgatagca agtgcaatca ggatttcatt tgaatgaagg cgtggattgc
    17581 ggctaccgag atgatcgatt ttaagacctt ggattggctt aacaacttca ggctcgatta
    17641 gttttacttc tttagcgatg tcagctgatt ttttgatggc attgatcaag gcagcggctg
    17701 tagggccaaa gagttctgag ttcttaccag tgatgatttc cccatttggc aattcaaagg
    17761 ctagggctgg tccaccagtt tcttctgctt tttggcgcgc aacgacagca accttacggt
    17821 ctgcaggtgt gataccgagg tcgttcatga gcaactcaat tttcttgacg gcagcttcgc
    17881 caactttttc agctttgaag tcaagaactg tttgatagta acggcggatg atttcttgtt
    17941 tagaagcttc gacagcagcc tcgtcatctg taatagcgaa accaaccatg ttgacaccca
    18001 tatctgtcgg tgaagcgtat ggtgattttc cgagaatacg ttccaacatg cgcttgagca
    18061 ctgggaagat ttcgatatca cggttgtagt tgacagtggt ttctccatag gtttgaagat
    18121 ggaaggggtc aatcatgttg acatcatcaa ggtcagctgt ggcagcttca taagccaagt
    18181 taactggatg atgaagggga agattccaaa cagggaaggt ttcaaattta gcgtagccag
    18241 atttgatgcc attgatttgg tcgtggtaca tattggacat acacgttgcc aattttccag
    18301 aaccgggtcc aggagcggtt acgacaatca agttgcgact ggttttgatg tagtcgtttt
    18361 tgcccatgcc ttctggggaa atgatgtgat ccatatccgt cggatatcct ttgattggat
    18421 aatgaagata agaatcaatt ccgtttttct caagttggtt gcggaaggca tctgcagcgg
    18481 gttggccagc gtattgtgta atgacaacgg aaccaacaaa aatccctaat tcattgaatt
    18541 tgtcaatcaa acgaagaact tcttggtcat aagaaatgcc caagtcgcca cgtgctttgg
    18601 aatgttcaat gttgctagca ttaatggcaa tcacaacctc aacctgctct ttcaattctt
    18661 gcaagagctt gattttgttg tcaggttcat aaccaggaag gacacgagca gcgtggaaat
    18721 cttctaacat tttgccacca aactctaggt aaagcttgcc gtcaaattgg ttaatgcgct
    18781 ctaagatgtg gtcgcgttgt aaattcaaat actgttcaga actaaaagct tgttttttca
    18841 tttttttacc tctggactct attataataa aaaattggaa gttaggaaac tacggagcta
    18901 aaaaataaat taaaaagatt aagcaaacgc ttgcacaaaa ttttaaaaag tgctatcata
    18961 tactatagat tattaaaata atgaggtaaa cagatgcaag aaaaatggtg gcacaatgcc
    19021 gtagtctatc aagtctatcc aaagagtttt atggatagta atggagatgg agttggtgat
    19081 ttgccaggaa ttaccagtaa gttggactat ctagccaagc taggaatcac agcaatttgg
    19141 ctttctcccg tttatgacag ccctatggat gataatggct atgatattgc tgattatcaa
    19201 gcgattgcgg ctatttttgg aaccatggag gacatggatc agctgattgc agaagctaag
    19261 aagcgtgaca ttcgtatcat catggacttg gtggttaatc atacctcaga tgaacatgct
    19321 tggtttgtcg aagcctgtga aaatactgac agccctgagc gagactacta tatctggcgc
    19381 gatgaaccca atgacctaga ttctatcttt agtgggtctg cttgggaata cgatgaaaag
    19441 tcaggtcaat actatctcca ctttttcagc aagaaacagc cggatctcaa ctgggaaaat
    19501 gaaaaacttc gccagaaaat ttatgagatg atgaacttct ggattgataa aggtattggt
    19561 ggtttccgta tggatgttat tgacatgatt ggcaaaattc ctgacgagaa ggtagtcaat
    19621 aatggtccta tgctccatcc ctatctcaag gaaatgaatc aggcgacctt tggagataag
    19681 gatctcttga cagtagggga gacttgggga gcaacgccag agattgccaa gctttactct
    19741 gatccaaagg ggcaagaatt gtctatggtc ttccagtttg aacatatcgg tcttcagtat
    19801 caggaaggtc agcctaaatg gcactatcaa aaagagctga atatcgctaa gttaaaagaa
    19861 atcttcaaca aatggcagac agagttagga gttgaggacg gttggaattc cctcttctgg
    19921 aacaatcatg acctccctcg tatcgtctca atctggggaa atgaccaaga ataccgcgaa
    19981 aaatctgcca aagcctttgc aatcttgctt catcttatga gaggaactcc ttatatctac
    20041 caaggtgagg agattgggat gaccaactat ccgtttgaaa cactggatca agtagaagat
    20101 attgaatctc tcaactatgc gcgtgaggct cttgaaaaag gtgttccgat tgaagaaatc
    20161 atggacagta tccgtgttat tggacgtgac aatgcccgta cccctatgca atgggacgag
    20221 agcaaaaacg ctggtttctc aacagatcaa ccttggttgg cagttaatcc aaattacgag
    20281 atgatcaacg tccaagaagc gctggcaaat ccagattcta ttttctatac ctatcagaaa
    20341 ctggtccaaa ttcgcaagga gaatagttgg ctaattcgag ctgactttga attgcttgat
    20401 acggctgata aggtctttgc ttatatacgt aaggatggcg accgtcgctt cctagttgtg
    20461 gctaacttgt ccaatgaaga gcaagacttg acagtagaag gaaaagtcaa atctgtcttg
    20521 attgaaaaca ccctagctca agaagtcttt gaaaaacaaa tcttagttcc atgggatgct
    20581 ttctgtgtgg aattactata aatatttttt gcagaaaaat ttaaaattga aatcatataa
    20641 aaacaaggga ggactgtata aaaggcggaa atcctttgtt ttttataacc aaggtttata
    20701 aactttcatt ctcgaaattc aattaacttt acaaattccc actattaagg agaaagaaga
    20761 cgaacataaa gaagcgtatc cttagtgcag gcctgacttt tgcatctgct ttgcttttag
    20821 ctgcttgcga ccaatcaggt tcagatacaa aaacttactc atcaaccttt agtggaaatc
    20881 caactacatt taactatcta ttagactatt acgctgataa tatagtcaat tgaaacaaga
    20941 gaaagacaaa agagcctcgt aaaaggtatt gcaacttggt aatacctttt tgaggtgctt
    21001 tttgatatga gcccatgttt tctcaatagg attgtactca ggtgagtagg gaggaagagg
    21061 taaaagttta tgcccaaact cttcgcataa aagttctagc ttccccattc tatggaatct
    21121 tgcattatcc ataataataa ccgatggtgt ggttaatgtt ggtaagaaaa acttctgaaa
    21181 ccaagcttca aaaaagtcgc tcgtcatcgt ctcttcgtaa gtcattggaa cgattaactc
    21241 accatttgtt agacctgcaa ccaaagaaat cctctgatat cttcttccag atactttgcc
    21301 tcttcttaac tgacctttta atgagcgacc atattctcga taaaaataag tatcgaatcc
    21361 tgtttcatca atctaaacag gtgctaggtg ctttaaacta ttaaaattct taagaaataa
    21421 ggctactttt tctgggtctt gttcataatt agattgaaac tagaatagta cacctctgct
    21481 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcatg ttcttatttc
    21541 attttactat atttttgttt cgcgggaagt ctactaagat acttaaagat gcagatagta
    21601 aaaaaaaatg tagacattac cgtaaaaaag tgatataatc gtatgatgtt caaggtatag
    21661 gtgttaatca tgagtagacg ttttaaaaaa tcacgttcac agaaagtgaa gcgaagtgtt
    21721 aatatcgttt tgctgactat ttatttattg ttagtttatt ttttattgtt cttaatcttt
    21781 aagtacaata tccttgcttt tagatatctt aacctagtgg taactgcgtt agtcctacta
    21841 gttgccttgg tagggctact cttgattatc tataaaaaag ctgaaaagtt tactattttt
    21901 ctgttggtgt tctctatcct tgtcagctct gtgtcgctct ttgcagtaca gcagtttgtg
    21961 ggactgacca atcgtttaaa tgcgacttct aattactcag aatattcgat cagtgttgct
    22021 gttttagcag atagtgagat tgagaatgtt acgcaactga tgagtgtgac agcaccgact
    22081 gggactgata atgaaaacat tcaaaaacta ctagctgata tcaaatcaag tcagaatacc
    22141 gatttgacgg tcaatcagag ttcgtcttac ttggcagctt ataagagttt gattgcagga
    22201 gagactaagg ccattgtctt aaatagtgtc tttgaaaata tcatcgaatc ggagcatcca
    22261 gattacgcat cgaagataaa aaagatttat accaagggat tcactaaaaa agtagaagct
    22321 cctaagacgt ctaaggatca gtctttcaat atctatgtta gtggaattga tacttatggt
    22381 ccgattagtt cggtgtcgcg ttcagatgtc aatatcctga tgactgtcaa tcgagatacc
    22441 aagaaaattc tcttgaccac aacgccacgt gatgcttatg taccaatcgc agatggtggg
    22501 aataatcaaa aagataaatt gacccatgcg ggcatctatg gagttgattc gtccatccac
    22561 actttagaaa atctctatgg agtggatatc aattactatg tgcgattgaa cttcacttct
    22621 ttcttgaaat tgatcgattt gttgggtggg gtagatgttt ataatgacca agattttaca
    22681 tctctacacg ggaagttcca tttcccagtt ggaaatgtcc acttagattc tgagcaggct
    22741 ctgggatttg ttcgtgagcg ctattcccta gcggatggtg atcatgaccg cgggcgcaat
    22801 caacaaaagg tgattgcggc catccttcaa aaattaactt cgagcgaagc gctgaaaaat
    22861 tatagtagga tcattgatag cttgcaagat tctatccaaa caaacatgcc acttgagacc
    22921 atgataaact tggtcaatgc tcagttagaa agtggtggaa cgtacaaagt gaattcgcaa
    22981 gacttgaagg gtatgggacg gacggatctt ccttcctatg cgatgccaga tagtaacctc
    23041 tatatgatgg aaattaacga cagtagcctt gcatctgtca aaactgctat tcaggatatg
    23101 ttggagggca gatgaaatga ttgatattca ttcgcacatc gtctttgatg tagatgatgg
    23161 tcccaagtca agagaggaaa gcaaggctct cttggcagaa tcctacaggc agggggtgcg
    23221 aaccattgtc tctacctctc accgtcgcaa gggcatgttt gaaactccgg aagagaagat
    23281 agcagaaaac tttcttcagg ttcgggaaat agctaaggaa gtggcgagtg acttggtcat
    23341 tgcttacggg gctgaaattt actacacatc agatgttttg gataagctgg aaaaaaatcg
    23401 gattccgacc ctcaataata gtcgttatgc cttgatagag tttagtatga acacccctta
    23461 tcgcgatatt catagtgcct tgagtaaaat attgatgttg ggaattactc cagtcattgc
    23521 ccacattgag cgctatgatg cacttgaaaa taatgaaaaa cgcgttcgag agctgatcaa
    23581 tatgggctgt tacacgcaag taaatagttc acatgtcctc aaatccaaac tttttggaga
    23641 acgttataaa ttcatgaaaa aaagagctca gtattttttg gagcgtgact tggttcatat
    23701 cattgcaagt gatatgcata atgtggacag cagacctccc catatggcag aagcatatga
    23761 ccttgtttcc caaaaatacg gagaagcgaa ggctcaggaa ctttttatag ataatcctcg
    23821 aaaaattgta atggatcaac taatttagga gaaatgatga aagaacaaaa cacgatagaa
    23881 atcgatgtat ttcaattact taaaaccttg tggaaacaca agctaataat tttattagtg
    23941 gcacttgtga caggggcggg agcttttgca tatagcactt ttattgttaa gccagaatat
    24001 acaagcacca cgcgtattta cgtagtcaac cgtaatcaag gagataagcc gggactgacg
    24061 aatcaggact tgcaggcagg aacttatctg gtaaaagact accgcgaaat tatcctttcg
    24121 caagatgtat tggaaaaggt agcgacaaat ttgaaattgg atattccagc aaaaacgtta
    24181 actagcaaag tgcaagtgac tgtaccagcc gacactcgta tcgtctcaat ctctgtcaag
    24241 gataaacaac cagaggaagc cagtcgcatt gctaattctc tacgagaagt tgctgcagaa
    24301 aagatcatcg ctgtaacgcg agtatctgat gtaacgacgc ttgaagaagc gcgaccagct
    24361 atgactccct cttctccaaa tgttcgacgc aacaccttag ttggttttct tggaggagcc
    24421 gccgtaacag taattactgt tcttttgatt gagttgctcg atacccgtgt aaaacgtcct
    24481 gaagatgttg aagatgtact gcaaatgcca cttctaggag tcgttccaga ttttgataaa
    24541 atgaaatagg aggaagttat gccaacgtta gaaatctcac aggcgaaatt agaacttgtg
    24601 aaaaaggcag aggaatatta taatgctttg tgcacgaacc tacagttaag tggagatgat
    24661 ttgaaagtat tttctatcac ttctgtgaaa gcaggagaag gaaaaacaac gacttccacc
    24721 aatatcgctt gggcttttgc gcgtgcaggt tacaaaacgc ttctgattga tgcagacatg
    24781 cgtaactccg ttatgtcagg tgtctttaaa tcaagggaaa ggattactgg actaacagaa
    24841 tttctatcag ggacaacaga cctgtcacag gggctttgcg ataccaatat tgagaatctc
    24901 tttgtgattc aggctggctc tgtgtcacca aatccgacag cccttcttca aagtaagaat
    24961 ttcagtacaa tgcttgaaac cttgcgtaaa tattttgact atatcgtcgt agacactccc
    25021 cctgtcggtg tcgtgattga tgcggctatc attacgcaga aatgtgatgc ttctatttta
    25081 gtgacgaagg caggtgaaac aaaacgacgg gatcttcaaa aagcgaaaga acagttggaa
    25141 caaactggga agtcgtgttt aggagttgtg ttgaataaac tcgatacttc agtagagaaa
    25201 tacggttctt atgggagtta tgggaattac aggaaacaga aaaaataggt tgagggatag
    25261 aaatgagtga aaaactagca aagccttcag tagccatagt ccagagtttt cttgttattt
    25321 tattgactta tctacttagc gctgtgagag aaacagagat tgtttcaaca acagctattg
    25381 tactttatat cctccactat tttgtctttt atatcagtga ttatggacgg aatttcttta
    25441 aaaggggata tttgattgaa cttgtccaga cattgaaata tatcctattc tttgcgctag
    25501 cgattagtat ttctaatttt ttcttagaag atcgatttag tatttccaga agaggcatga
    25561 tttacttcct cttattacat gttttcttag tctatgtgct aaacccattt atcaagtggt
    25621 attggaagcg ggcttatccc aactttaaag gaagtaagaa gattctccta cttacagcaa
    25681 cttctcgtgt tgaaaaggta ctggatagat taatagaatc aaatgatgtt gttggggagt
    25741 tggtagccgt cagtgtttta gataaaccag attttcagca tgatgattta aaggttgtag
    25801 cagaggggga gatagtaaat tttgcgactc atgaggtggt cgatgaagtc tttatcaatc
    25861 ttccaagtga aaaatacaat attggagagc ttgtctctca gtttgaaacg atgggaattg
    25921 atgtaacagt caatctaaat gctttcgatt gggcacgtaa caagcaaatt tgtgagatgg
    25981 caggactaaa tgttgtgact ttttctacaa cattttataa gactagccat gtgattgcta
    26041 agcggattat tgatattgtc ggttccctgg taggtttgat attatgtggt ctagtcagta
    26101 ttgtgctggt tcctttgatt cgaaaggatg ggggttctgc tttttttgct cagacgcgta
    26161 taggaaaaaa tggccgccat tttacttttt ataagtttcg ctctatgtgt gtggatgctg
    26221 aggagaaaaa aagagaactc atggaacaaa ataccatgca gggtggaatg tttaaggtgg
    26281 acgatgatcc tcgtatcacg aaaattggtc attttatacg gaagacgagc ttggacgagc
    26341 taccacagtt ttataatgtt ctaaagggag atatgagttt ggtaggtaca cgaccaccaa
    26401 cagtggacga gtatgagcac tataccccag aacaaaaacg tcgcctaagt tttaaacctg
    26461 gcataacagg tttatggcag gtcagcggac gaagtgaaat caaaaatttc gatgaagttg
    26521 tcaaattaga tgtggcctat attgatggtt ggacaatctg gaaagatatt gaaattttat
    26581 tgaagacagt taaggttgta ttgatgaagg atggagcgaa atagttcata tgaatgagag
    26641 aattcaaata ttaggaatta ctatagatcc attaacgatg aaagaaacag tggatgctgt
    26701 tgaacagtat gttttaaaga agcacccttt gcacttgatg ggagttaacg cggataaaat
    26761 taatcagtgc tatgaagatg agaaaatcaa aaaaatcgtt aatgaatcag gaataattaa
    26821 tgccgacggt gcttcagtag ttttagcgag taagttttta ggaacaccag ttcctgagcg
    26881 agttgctgga atagacttga tgaaacattt actagagttg tctaatgaaa aaggatactc
    26941 ggtctacttt tttggagcaa aagaagacgt tttacaagat atgttaaaaa tttttgaaga
    27001 aagatatcca gctctaaatg ttgttggcta tagaaatgga tatttttcgc ctgaagatga
    27061 aaaacatatt caggaagata ttaaagagaa aaaaccagat tttgtgtttg taggaattac
    27121 ttctcctaag aaagaatata ttatccaatc attcatggat aatggaatta atgctgtgtt
    27181 tatgggggtt ggtggaagtt ttgatgtact atcagggcat atcaaacgag ctccattatg
    27241 gatgcaaaat gctcacctag agtggttgtt tcgtgtggca aatgagccta aacgtctctt
    27301 taagcgttat tttgtaggga atgtttcatt tataagaaaa gttttaagag cgaaaagggg
    27361 atataaactt tgaaccaaac agaaatagtt aaattgattc agaaagttga attggatgct
    27421 ataaaagagt ttcaaaaaat ttgtaaagag aacaatatag atttcttcct tcgtgggggg
    27481 agtgtacttg gggcagtcaa atatgatggc tttattccat gggatgatga catggatatt
    27541 gctgtgcctc gtgaaggcta cgataaactt ccaggtatct ttaaggatag aattatcgca
    27601 ggcaaatatc aggttcttgc ttatcaatat tgcgatactt tacattgcta ctttcctcgc
    27661 ttattccttt tagaagatga aagaaaacgt ttgggattgc cacgaaatac caatttaggt
    27721 ttgcatttga ttgatatcat tcctttggat ggtgccccaa atcattcatt tttaagaaag
    27781 ctttacttcg gtaaagtata ctggtatcgt tttttagcaa gtttaggaac tacctatgtt
    27841 ggagaccatg tggatatgca ttccgctaag caaaaactaa tcattggttt ctttaaaaag
    27901 ctaggatttg caaaactatt ccctcaaaat tatgtgtaca gacgcttgga taatctctat
    27961 aaaaagtatg attggaaaaa gcagaagtat gctgggacaa ttaatgcatc tctgtttgcc
    28021 aaagaagtca tgccagtaga gatttgggga gaaggagtag agaagccttt tgaggatgcc
    28081 ttctttaaag ttcccacgga gtatgaccgc tatctgaaaa gactttacgg agaaaactat
    28141 cttcacgaag agcctagtga tgatgaaaag aaatcgcatt taggaggaca ataatttgtt
    28201 ttgttatatt attttgcatt acaaagtctt agaggaaact atttcttgtg ttaaatcaat
    28261 aaaagaaggc aattccaata caaagcagat cgttattatt gataattttt ctaataacgg
    28321 tacgggtgaa aaactacaag agctgtatga gtcagattca gagattgatg tcttgattaa
    28381 ccatgaaaat gctggtttcg ctcgaggtaa taatgtagca tatcagtttg ctaaggaaaa
    28441 gtacaaacct gattttatgg ttatcatgaa taatgatatt gagatagaaa cagaagagtt
    28501 tgaaaaaatc gtgacagata tctatcggaa ggaaaaattc catttgttag gaccagatat
    28561 cttttcgacg acgtatcagc ttcaccaaaa cccaaaacgg ttgacgcatt atacttatga
    28621 agaggttaag gctctcaatg aaaaatttaa gaaagggagc caagttagtc tagcattaaa
    28681 aattaaatgt tggttgaagt ctagtaaagt tcttcggaca gcaatctatc aaaataggcg
    28741 taaaaagaaa tcagtagact atagaaaaca ggtagaaaac ccaattcttc atggttcgtt
    28801 tattgtatat tctagagatt ttattgagaa agaggagtat gcttttaatc ccaatacctt
    28861 cttctattat gaaacagaga tattagatta tgaagctgag ttaaaaggat ataagagaat
    28921 ttatacaccg aagattaagg tcttgcacca tcaaaatgtt gcaaccaatc aagtctatac
    28981 aaacttagta gaaaaaacct tgttttcaaa caaatgcaac tttgaatcca ctagttattt
    29041 tttgaagttg atgaaagaaa acgagggtgt gtaaatgact tatttatttt tactctgcct
    29101 gaccttattc ttattaactt tcttctattt ttttgctttc aatcaagatt tgatagctcc
    29161 gccagttgtc atgtctgtta tgtttctcat tagttctgta tttgccctta ttaatgtgca
    29221 aaactggaat attgagtata gtggtttagc ttatcttttg attattagtg gcattattgt
    29281 tttttcaatg cctctcttgg ctcttaattc gcctagtcta aataccaaga ttaaagtgac
    29341 ggatcggctc attgatatcc aattctggaa aatagctctt actattatag ttgacctcgt
    29401 tattctatat ctttatagga gagagattca taatcttgca cttagccatg gttatacggg
    29461 ttcaaatttt cagtggttct ttagaaatgc taccagttat gaaggtgagc taacagtgcg
    29521 aacttcgatt cgggtcctca ttcgtatcat tgacgtatct gcttatattt ttggatatac
    29581 ttttattaat aatttcttca tttatagtca taaacgctct aaagatttac tgctcttagt
    29641 tccattcttg atttttattt ctaaaacctt attatctggg ggtagattgg atattataaa
    29701 aattttaatt gcgtatgttg taatggccta tattcagcaa aaacgaaaag ttggctggga
    29761 taaggtcatc tcccataaat atatgagact tggttttgta ggcttgatag ctgggattcc
    29821 taccttttac tattctttat tcttatctgg acgttctacg actagaactg tatttgaaag
    29881 tatttcaacc tatttaggag gttcgattca gcattttaat cagtatattc aaaatcctat
    29941 tggagtagct gaggtttttg gcgatgagtc atttgtagct attatgaata ttttgggtaa
    30001 tcttggcttt gtcaattaca atagtaccgt tcacttagaa tttcggcagt tagggattac
    30061 tatgggtaac gtttatactt tctttagaag accctggcat gactttgggt tagtcggtat
    30121 gtatatcttt tcctttgttg tcggtgtgtt ttttgctatt ttttatttga aattaagaaa
    30181 gagccgtgct ggttttaaat
//
LOCUS       DAVMHG010000008        29335 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMHG010000008 DAVMHG010000000
VERSION     DAVMHG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.008"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(725..817)
                     /locus_tag="VYR08_000284"
     CDS             complement(725..817)
                     /locus_tag="VYR08_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV3465871.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            978..1949
                     /gene="ruvB"
                     /locus_tag="VYR08_000285"
     CDS             978..1949
                     /gene="ruvB"
                     /locus_tag="VYR08_000285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV3465872.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            2038..2502
                     /locus_tag="VYR08_000286"
     CDS             2038..2502
                     /locus_tag="VYR08_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV3465873.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQFYMHQHSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2733..3491
                     /locus_tag="VYR08_000287"
     CDS             2733..3491
                     /locus_tag="VYR08_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV3465874.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3500..4303
                     /locus_tag="VYR08_000288"
     CDS             3500..4303
                     /locus_tag="VYR08_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV3465875.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4325..5584
                     /gene="rseP"
                     /locus_tag="VYR08_000289"
     CDS             4325..5584
                     /gene="rseP"
                     /locus_tag="VYR08_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV3465876.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5597..7450
                     /locus_tag="VYR08_000290"
     CDS             5597..7450
                     /locus_tag="VYR08_000290"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV3465877.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7550..8929
                     /gene="bglA"
                     /locus_tag="VYR08_000291"
     CDS             7550..8929
                     /gene="bglA"
                     /locus_tag="VYR08_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV3465878.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            9121..10929
                     /gene="glmS"
                     /locus_tag="VYR08_000292"
     CDS             9121..10929
                     /gene="glmS"
                     /locus_tag="VYR08_000292"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV3465879.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVAPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(11066..11143)
                     /locus_tag="VYR08_000293"
                     /pseudo
     CDS             complement(11066..11143)
                     /locus_tag="VYR08_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11200..12249
                     /locus_tag="VYR08_000294"
     CDS             11200..12249
                     /locus_tag="VYR08_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV3465880.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKIGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12349..16146)
                     /gene="spuA"
                     /locus_tag="VYR08_000295"
     CDS             complement(12349..16146)
                     /gene="spuA"
                     /locus_tag="VYR08_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV3465881.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPVTPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16563..16976
                     /gene="rpsL"
                     /locus_tag="VYR08_000296"
     CDS             16563..16976
                     /gene="rpsL"
                     /locus_tag="VYR08_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV3465882.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16996..17466
                     /gene="rpsG"
                     /locus_tag="VYR08_000297"
     CDS             16996..17466
                     /gene="rpsG"
                     /locus_tag="VYR08_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV3465883.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17891..19972
                     /gene="fusA"
                     /locus_tag="VYR08_000298"
     CDS             17891..19972
                     /gene="fusA"
                     /locus_tag="VYR08_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV3465884.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            20076..24467
                     /locus_tag="VYR08_000299"
     CDS             20076..24467
                     /locus_tag="VYR08_000299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV3465885.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24569..24832
                     /locus_tag="VYR08_000300"
     CDS             24569..24832
                     /locus_tag="VYR08_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV3465886.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24825..25103
                     /locus_tag="VYR08_000301"
     CDS             24825..25103
                     /locus_tag="VYR08_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV3465887.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            25124..25276
                     /locus_tag="VYR08_000302"
     CDS             25124..25276
                     /locus_tag="VYR08_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465888.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            25298..26539
                     /locus_tag="VYR08_000303"
     CDS             25298..26539
                     /locus_tag="VYR08_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV3465889.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRVYEEDPVKAWENHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAINAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIIDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26549..26779
                     /locus_tag="VYR08_000304"
     CDS             26549..26779
                     /locus_tag="VYR08_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3465890.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            27047..27769
                     /locus_tag="VYR08_000305"
     CDS             27047..27769
                     /locus_tag="VYR08_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3465891.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27987..29321
                     /gene="pepC"
                     /locus_tag="VYR08_000306"
     CDS             27987..29321
                     /gene="pepC"
                     /locus_tag="VYR08_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV3465892.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGSLAE"
BASE COUNT         8298 a         5575 c         6898 g         8564 t
ORIGIN      
        1 cctaaacttt tttcatcaca atctcctaat gaagccaccc aatcaggtgg cttttttgcg
       61 tggtgaggtg ctggtgatag aaattttttg caaaatagta aaattttaga aaagttaagc
      121 tagttttaag tttcgtcttg tatcatgtag ttattaaata aagacctcct aactttattt
      181 aataaaatcc taaacttttc tttttcataa taatctccct taacttcacc caatcaggtg
      241 gagtttttta gctctatttc aggcttttgg ggactattct aaaaataatt tttcgatatt
      301 tttcggtatt tttcggattt tggtcgggga attggcgggg acttttttag cgaatataac
      361 taagaaatag gtctgtggtc gcttcagcta gttcaaactc aatttgattg taagtgactg
      421 ttaattgaag aactggaact actgtaataa tgattttgct tgtctagtag tggcgtatta
      481 aatgagaagt aacatgtaag attgtctctt cgtttacgaa atcaaaattt ctatgaaatt
      541 gatagaatcc ctaacgttct cttaatctta accgtcttgt cgtacagcgg ttgaagaaaa
      601 tgtaaacctt tgacaaaatt cgtgaactat gggataataa aaaggaattg agtactagtt
      661 ctatatcata ggctagaaaa gaccccaagc tcacgaccaa ataagcttga ggtctttttt
      721 gacactattt gtccttgttt agccatttat cgactatacg aagaacgaca ccgaccacaa
      781 ttggtccgat gatagttttg agtactaatt ccatcatggg ctatctcacc tcctttcgta
      841 ggcggtgtag cagtgtcgta gaatattata ccacataagt gctaaactta ggagtcaccc
      901 aatcaggtgg cttttttgtt tgtggcttgg ttttttgata taatagagcc atgagtagaa
      961 ttttagataa tgagataatg ggggatgagg agttagtaga acgcacgctc cgtcctcagt
     1021 atttacgtga atatatcgga caggataagg tcaaggacca gctacaaatc tttattgaag
     1081 ctgccaaaat gcgggatgaa gcgctggatc atgtgctctt atttgggcct ccaggtctcg
     1141 ggaaaacgac catggccttt gttattgcca acgaactggg agtcaatctt aagcagacgt
     1201 cgggtccagt cattgaaaaa gccggagatc tggtagctat tttgaatgag ttagagcctg
     1261 gggatgtact ttttattgat gagatccatc gtttgccaat gtcagtggaa gaggtgcttt
     1321 atagtgctat ggaggacttc tacatcgata ttatgattgg ggctggtgag ggtagtcgta
     1381 gtgttcattt ggagttacca ccttttacct tgattggtgc gacgactcgg gctggtatgc
     1441 tctccaatcc gctacgggca cgttttggga ttacaggcca tatggagtat tatgcccatg
     1501 ctgacttgac agaaattgtc gagcggacgg cagatatttt tgagatggaa atcactcatg
     1561 aggcagcatc tgagttggcc ctacgtagtc gtgggacccc tcgtattgcc aatcgtctcc
     1621 tcaagcgcgt gcgcgatttt gcccagataa tggggaatgg ggtaattgat gatattatta
     1681 ccgataaggc tttgactatg ctggatgttg accatgaagg tttggactat gtggatcaaa
     1741 aaatccttcg taccatgatt gagatgtaca gtggaggacc tgttggtcta ggaactcttt
     1801 ctgtgaatat cgccgaagag cgtgagacag ttgaagatat gtatgagcct tacttgattc
     1861 aaaaaggttt tatcatgcgg acacggtctg gacgggtggc gactgctaag gcatatgagc
     1921 acttaggtta tgaatacagt gaaaaataag caagaaatct tagaggcttt tagagaaaat
     1981 ccagatatga tggccattct gacgatcatc cgagaccttg gtttgaaaga ctcgtagttg
     2041 gcagcaggtt ctgtcagaaa tttcatctgg aatcttttgt cagacaaatc cccttttgat
     2101 catgaaacag atatagatgt gattttcttt gatccagatt tttcttatga ggaaacctta
     2161 ttactggaga aaaagctgag agaggatttt cctcagtacc agtgggaatt gaaaaatcag
     2221 ttctatatgc atcagcacag tcctcacact gctttctata ccagttctcg tgatgctatg
     2281 agtaagtatc cagaacggtg tacggcagtt ggactgcgct tgaatgaaga atcagatttt
     2341 gaactctatg caccttatgg tttggaggat attttgaatt ttcaagttcg tccaactcct
     2401 catttcttag aaaatgaaga ccgaatggaa ctctatcaaa cacgtttatc caagaaaaat
     2461 tggcaggaga aatggaaaaa tttgattttt aaaaatactt aaggaaactt taagctaggg
     2521 agtgtacact aagttcataa gttaagaaga ccttaactta aactcctaaa actttttcat
     2581 aataatctcc ctataaaaat aaagtcgccc aatcaggcgg cttatttttt tgaaaaatgg
     2641 gcatggtgcc tgagaataaa tagcttagtg atagaagaaa atggggaaat atggtataat
     2701 gaaacgatag atttttgaat aggaataaga tcatgtttgg attttttaag aaagataagg
     2761 ctgtggaagt agaggttccg acacaggttc ctgctcatat cggcatcatc atggatggca
     2821 atggccgttg ggctaaaaaa cgtatgcaac cgcgagtttt tggacataag gcgggcatgg
     2881 aagcattgca aaccgtgacc aaggcagcca acaaactggg cgtcaaggtt attacggtct
     2941 atgctttttc tacggaaaac tggacccgtc cagatcagga agtcaagttt atcatgaact
     3001 tgccagtaga gttttatgat aattatgtcc cggaactaca tgcgaataat gttaagattc
     3061 aaatgattgg ggagacagac cgcctgccta agcaaacctt tgaagcttta accaaggctg
     3121 aggaattgac taagaacaac acaggattga ttcttaattt tgctcttaac tatggtggac
     3181 gtgctgagat tacacaggcg cttaagttga tttcccagga tgttttagat gccaaaatca
     3241 atccaggtga catcacagag gaattgattg gcaactatct ctttacccag catttgccta
     3301 aggacttacg agacccagac ttgattatcc gtactagtgg agaattgcgt ttgagcaatt
     3361 tccttccatg gcagggagcc tatagtgagc tttattttac ggacacctta tggcctgatt
     3421 ttgacgaagc ggccttgcag gaagctattc ttgcctataa tcgtcgtcat cgccgatttg
     3481 gaggagttta ggaggaaata tgacccagga tttacagaaa agaaccttgt ttgcagggat
     3541 tgccctggct attttcctac caattttaat gattgggggc ctcttgcttc agatagcaat
     3601 cggaatcata gccatgctag ccatgcatga acttttgaag atgagaggtc tagagaccat
     3661 gacgatggag ggcctcttga ccctctttgc aacctttgca ttgaccattc ccttggagaa
     3721 ttacctgact tttttgccag ttgatgggaa tgtggttgcc tatagtgttt tgatttcaat
     3781 catgttagga acgaccgttt ttagcaagtc ttatacgatt gaggatgcgg ttttccctct
     3841 tgctatgagc ttctacgtgg gctttggatt taatgcttta ctagatgctc gtgttgcagg
     3901 tttggacaag gctctcttag ccttgtgtat cgtctgggcg acagacagtg gtgcctatct
     3961 tgttgggatg aactatggga aacgaaagtt agcaccaagg gtatcgccta ataaaaccct
     4021 tgagggtgcc ttgggtggta ttttaggagc aattttagta accattatct ttatgatagt
     4081 tgacagtaca gttgctcttc catatggaat ttacaagatg tcagtctttg ctattttctt
     4141 tagcattgct ggacaatttg gtgatttact agaaagttcg atcaaacgtc attttggtgt
     4201 taaggattct gggaaattta tccctggaca tggtggtgtt ttggatcgtt tcgatagtat
     4261 gttgcttgta tttccaatca tgcacttatt tggactcttt taatcaaaag acggaggaaa
     4321 cgctatgctc ggaattttaa cctttattct ggtttttggg attattgtag tggtgcacga
     4381 gttcgggcac ttctactttg ccaagaaatc agggatttta gtacgtgaat ttgccatcgg
     4441 tatgggacct aaaatctttg ctcacattgg caaggatgga acggcctata ccattcgaat
     4501 cttgcctctg ggtggctatg tccgcatggc cggttggggt gatgatacaa ctgaaatcaa
     4561 gacaggaacg cctgttagtt tgacacttgc tgatgatggt aaggttaaac gcatcaatct
     4621 ctcaggtaaa aaattggatc aaacagccct ccctatgcag gtgacccagt ttgattttga
     4681 agacaagctc tttatcaaag gattggttct ggaagaagaa aaaacatttg cagtggatca
     4741 cgatgcaacg gttgtggaag cagatggtac tgaggttcgg attgcacctt tagatgttca
     4801 atatcaaaat gcgactatct ggggcaaact gattaccaat tttgcaggtc ctatgaacaa
     4861 ttttatctta ggtgtcgttg ttttttgggt tttaatcttt atgcagggtg gtgtcagaga
     4921 tgttgatacc aatcagttcc atatcatgcc ccaaggtgcc ttggccaagg taggagtacc
     4981 agaaacggca caaattacca agattggctc acatgaggtt agcaactggg aaagcttgat
     5041 ccaagctgtg gaaacagaaa ccaaagataa gacggcaccg actttggatg tgactatttc
     5101 tgaaaagggg agtgacaaac aagtcactgt tacacccgaa gatagtcaag gtcgttacct
     5161 tctaggtgtt caaccggggg ttaagtcaga ttttctatcc atgtttgtag gtggttttac
     5221 aactgctgct gactcagctc tccgaattct ctcagctctg aaaaatctga ttttccaacc
     5281 ggatttgaac aagttgggtg gacctgttgc tatctttaag gcaagtagtg atgctgctaa
     5341 aaatggaatt gagaatatct tgtacttctt ggcaatgatt tccatcaata ttgggatttt
     5401 taatcttatt ccgattccag ccttggatgg tggtaagatt gtgctcaata tcctagaagc
     5461 catccgccgc aaaccattga aacaagaaat tgaaacctat gtcaccttgg ccggagtggt
     5521 catcatggtt gtcttgatga ttgctgtgac ttggaatgac attatgcgac tcttttttag
     5581 ataatcgagg aatattatga aacaaagtaa aatgcctatc ccaacgcttc gcgaaatgcc
     5641 aagcgatgct caagttatca gccatgctct tatgttgcgt gctggttatg ttcgccaagt
     5701 ttcagcaggt gtttattctt atctaccact tgccaaccgt gtgattgaaa aagctaaaaa
     5761 catcatgcgc caagaattcg aaaagattgg tgctgttgag atgttggctc ctgcccttct
     5821 tagtgcagaa ttgtggcgtg aatcaggtcg ttacgaaacc tatggtgaag acctttacaa
     5881 actaaaaaac cgtgaaaaat cagactttat cttaggtcca actcatgaag aaacctttac
     5941 agctattgtc cgtgattctg ttaaatctta caagcaattg ccactcaacc tttatcaaat
     6001 tcagcccaag tatcgtgatg aaaaacgccc acgtaatgga cttcttcgta cacgtgagtt
     6061 tatcatgaag gatgcttata gtttccacgc taactatgat agtttggata gtgtttatga
     6121 tgagtacaaa gcagcctatg agcgtatttt cactcgtagt ggtttagact tcaaggctat
     6181 tattggtgac ggtggagcca tgggtggtaa ggatagccaa gaatttatgg ccattacatc
     6241 tgctcgtaca gaccttgacc gctgggttgt cttggacaag tcagttgcct catttgacga
     6301 aattcctgca gaagtgcaag aagaaatcaa ggcagaattg ctcaaatgga tagtctctgg
     6361 tgaagatacc attgcttact caagtgagtc tagctatgca gctaacttag aaatggcaac
     6421 aaacgagtac aaaccaagca accgtgttgt cgctgaagaa gaagttactc gtgttgcaac
     6481 gccagatgtt aaatcaattg atgaagttgc agccttcctc aatgttccag aagaacaaac
     6541 gattaaaacc ctcttctaca ttgcagatgg cgagcttgtt gcagcccttc tagttggaaa
     6601 tgaccaactc aacgaagtca agttgaaaaa tcacttggga gcagatttct ttgacgttgc
     6661 tagcgaagaa gaagtggcga atgttgttca agcaggattt ggttcacttg gaccagttgg
     6721 tttgccagag aatattaaaa ttattgcaga tcgtaaggtg caagatgttc gcaatgcagt
     6781 tgtcggtgct aacgaagatg gctaccactt gactggtgtg aacccaggcc gtgattttac
     6841 tgcagaatat gtggatatcc gtgaagttcg tgagggtgaa atttccccag atggacaagg
     6901 tgtccttaac tttgcgcgtg gtattgagat cggtcatatt ttcaaactcg gaactcgcta
     6961 ttcagcaagc atgggagcag atgtcttgga tgaaaatggt cgtgctgtgc caatcatcat
     7021 gggatgttac ggtatcggtg tcagccgtct tctttcagca gtgatggagc aacacgctcg
     7081 cctctttgtt aacaaaacgc caaaaggtga ataccgttac gcttggggaa tcaatttccc
     7141 taaagaattg gcaccatttg atgtgcattt gattactgtt aatgtcaagg atgaagaagc
     7201 gcaagccttg acagaaaaac ttgaagcaag cttgatggga gctggttatg aagtcttgac
     7261 agatgaccgt aacgaacgtg tcggtgttaa atttagcgat agtgacttga ttggtcttcc
     7321 aatccgtatc actgttggta aaaaagcggc cgatggcatt gtagaagtta agatcaaggc
     7381 aactggtgac accattgagg ttcatgcaga taacgtgctt gaaacgcttg aaatcctaag
     7441 caagaaataa aaactataat cagaagaaaa acaaggaaaa aatgtaacta gtttttacct
     7501 tgttttttct gtataatggg agaaatgagt agataaagag gtaaatgtta tgctaagatt
     7561 tccaaaggat tttgtctggg gatcctctac ttctggaccg cagacagaag gacgtgtagc
     7621 tggtgacggt aagggagaca atctctggga ttactggttc caagtggagc caaatcgtta
     7681 ctataatggg attggttcag ataagacatc gactttttat gaaaattggg agcgggatat
     7741 tgagcttttg ttagagactg gtcacacagt ctttcggact tctattcagt ggtcacggat
     7801 ttttccacaa ggctgtggga aagtcaaccc tcaaggtgtg gatttttatc gtaaggtctt
     7861 tgaggctatt aaggctaaag gaattcgtct gttagtcaat ctctatcatt ttgatttacc
     7921 ttttgccctt caagaggatg gtgatggttg ggaaaataag gcgacagtct cagcctatga
     7981 agactatgct cgtttttgtt ttgagactta tggagattta gtggatcaat ggattacctt
     8041 taacgagccc atcgttcctg tagaatttgg ttatttttat gatgcccatt atccacataa
     8101 ggtggatgca gaggcagctg ttaaggtagc ctatcataca caattggcca gcagccgggc
     8161 tgttaaggct tgccatgaac ttttgcctga ttccaagatt gggattgtcc tcaacttgac
     8221 accggcttat ccacgtagcc agcatcctgc tgatgtcaag gcagctcgta ttgcggccct
     8281 ttttcaggcc caatctttct tagatccatc tgtcttgggg acttatccac aggagttggt
     8341 agaaatcttg catgaacacg gtcttttacc tgatgctaca gaggaagagt tggaactcat
     8401 tcgtgataat acggtggact tccttggtgt gaactactat caacctttgc gtgttatggc
     8461 tcctcgattt gctaagcatc cagagagtcc actcttacca gaacattttt acgagcctta
     8521 tgtgatgcct ggacgtaaaa tcaattctca ccgtggttgg gagatttatg agcaagggat
     8581 ttatgacatc gcccaaaata tcaaggaaaa ttatggcaat attgaatgga tgttgactga
     8641 gaatggtatg ggtgttgaag gggaagaaaa attccgtcaa gatggaatga ttcaagatga
     8701 ttaccgtatt gactttgtaa aaggtcatct tcgtgaactt caccgtgcca ttgaagatgg
     8761 tgccaactgt aagggctact tgatttggac ctttattgac tgctggtcat ggctcaacag
     8821 ctataaaaat cgctatggtt tggtcgaatt agacttggaa acgcaagaac gtcgtctgaa
     8881 gaaatcaggc cactggttca aaaaattaag cgataataat ggattttaaa aaggactctg
     8941 actgattatc ccaatcggtc agagtttttt gcttacagga gcttaatttg aactatacca
     9001 atttttactc ttgacaagtg aaagaaacaa gtatatactg tttttgctga ttctataaaa
     9061 gatgaattag actataccaa ttttaaggag aaagcacagc ttgcctgtgt cgtatatact
     9121 atgtgtggaa ttgttggtgt tgttggaaac acaaatgcaa ctgatatttt gattcaaggg
     9181 cttgaaaagc ttgaataccg tggctatgat tctgcgggaa tttttgtcct agatggtgct
     9241 gataaccatt tggtgaaggc tgttggtcgt attgcagaat tgtctgccaa gacagctggt
     9301 gttgagggaa caactggtat cggacatact cgttgggcta ctcacggaaa accaactgag
     9361 gacaatgctc acccacaccg ctctgagaca gaacgttttg tcttggtgca taatggggtg
     9421 attgagaact accttgaaat caaggaagaa taccttgcag gtcaccactt caaggggcag
     9481 acagatactg aaatagccgt tcatttgatt ggaaaatttg cggaagaaga agggctctca
     9541 gttcttgaag ccttcaaaaa agcccttcac atcatccgtg gttcttatgc ctttgccttg
     9601 gttgactcac aagatcctga agtcatctac gtagctaaaa ataaatcacc acttttgatt
     9661 ggtcttggag aaggctataa catggtctgc tcagatgcta tggctatgat tcgtgaaacc
     9721 aaccaataca tggaaattca tgatcaagag ttggtaatcg tcaaggctga tagcgtggaa
     9781 gttcaagact atgatggtaa cagtcgtgaa cgtgctagct atactgcgga acttgacttg
     9841 tcagatatcg gtaagggaac ttatccttac tacatgctta aggaaattga tgagcaacca
     9901 actgttatgc gtaaactcat tcaagcctac acggatgatg ctggtcaagt agtggttgct
     9961 cctgctatca ttaaggctgt tcaagacgca gaccgcatct acatccttgc agctggaaca
    10021 tcttaccatg caggatttgc ttctaagaaa atgttggaag aattgacaga tacaccagtt
    10081 gaacttggaa tctcatctga gtggggctac agtatgccac ttctcagcaa gaaaccactc
    10141 ttcatcttta tcagccaatc tggtgaaaca gcggatagtc gtcaagtttt ggtcaaggct
    10201 aatgaaatgg gaattccaag cttaacagtg acaaatgttc caggttcaac cctctcacgt
    10261 gaagccaact ataccatgct ccttcacgca ggtcctgaaa ttgctgtggc atcaactaaa
    10321 gcctatacag cgcaaatcgc agcccttgcc ttccttgcaa aagcagtcgg agaagcaaat
    10381 ggtaatgcta aagcgcaagt ctttgatctg gttcatgaat tgtcaatcgt agctcagtct
    10441 attgaatcaa ctctttcaga gaaagaaacc attgaagtca aggttcgtga acttcttgaa
    10501 acaactcgta acgcctttta catcggacgt ggtcaagatt actacgtagc catggaagca
    10561 agtctcaaac tcaaagagat ttcttatatc cagtgtgaag gctttgctgc aggagaactc
    10621 aagcacggaa ccattgcctt gattgaagaa ggaacgcctg ttttggctct cttgtcagat
    10681 ccagttcttg ccaaccatac tcgtggaaat atccaagagg tcgcagcccg tggtgccaaa
    10741 gtcctcacta tcgcagaaga gaatgtagcc aaagataccg acgatatcgt ccttacgacc
    10801 gtacatccat acctctcacc aatttcaatg gtcgtaccaa cgcaattagt cgcttacttt
    10861 gcaaccctcc accgtggcct cgatgtggac aaaccacgta accttgccaa gtcagtaacg
    10921 gtagaataag ctaaaaaagt ctagttatct aggctttttc ttgtgagcaa attggttgct
    10981 tgtactcaag attcgtgtta tactcttcga aaatctcttc aaaccacgtc aacgtcgcct
    11041 tgccgtaggt atggttactg acttcgttag ttctatccac aacctcaaaa cactgttttg
    11101 agcagcctgc agctagcttc ctagtttgct ctttgatttt cattgagtat tagaataatt
    11161 tttcaaattg aaggacagag atagacaagg agaatcacaa tggtagaatt gggaatttca
    11221 acatttgggg aaataacgga gcttgaaggg actggacaaa cttacagtca tgctgaacgc
    11281 attcgccagt tggtggcaga gattgagctg gctgacaaga ttggtttgga cgtatatggg
    11341 attggcgagc accatcgggc ggattttgca gtatcagccc cagagattgt cctggccgct
    11401 ggggcagtca ataccaagaa aatccgtttg accagtgcag tcagcattct gtcaagcatg
    11461 gatccgattc gtttgttcca acagtatgcc actatcgatg ctttgtcaaa tggacgatcg
    11521 gagattatgg ctggaagggg ctctttcacg gaatctttcc ctctgtttgg ttatgacttg
    11581 aaagactacg actctctttt tgatgagaaa ttagacttgc ttcagttagt caatgaaaag
    11641 accaagttag actggcaagg tcgattgact caaacgattg ctggtaaaga agtttatcct
    11701 cgtccagttc aggacaaatt gcccttgtgg atagctacag gtggtcatgt cgaatcaaca
    11761 gtgaagattg ctcaggctgg tctaccgatt gtatatgcta ttattggtgg taatccacgt
    11821 tattttaaaa agttgattca ggcttatcgt gagattggaa gcgaagcggg ccatgctgac
    11881 aaagacttga aagtgggagc ccattcttgg gggtggattg cggaagatgg tgagcaggcg
    11941 gtgaaagatt atttccatcc gactaagcaa gtggtggatg ctatttccaa agaccgtccg
    12001 cactggcagg aattgcgtta tgagcaatat ttggagcagg tagggccaaa tggcgccatg
    12061 tttgttggca atccagatca ggtggccgaa aaattgattc gcatgataga ggatttagga
    12121 ttggaccgct tcatgcttca tctaccgctt ggttctatgc ctcatgacca ggttctgaga
    12181 gctattgaac tcttcggaac acaagttgca cccaaagtac gagcttattt tgctatgaaa
    12241 gaggcttaat aaaaaatcct aatcaagttt attaggacaa caaatattgt acgaaaatta
    12301 atcccccgag ctataatgct tgggggattt ttctatagga gggctagttt agttctcttt
    12361 tttatttttt agtaagaaac cgagaccaag gagcgcaagg aggctgattc ctgcaaatag
    12421 gagtttgttt tcgtttttga ttcctgtatt tggaagttca gcttgtttat ctggggttac
    12481 aggtatattt tccttgctag agttttctac cgattcgttt cgaaccgctt cttttacaga
    12541 tgatgcttgt gcttcttgtg ctggttgttc agcttgtgaa tcagctgtag cttggctagg
    12601 tttattttcc gcatcagcta ctttggcatc tggtttagta gaatcaggtc tagcttctgg
    12661 agctgggtct tgatgtgcag ggtgagaagc ttcattttga tgttcaggct tggaaggggt
    12721 tgagtctggt ttctcttctg cagttgactc atggctagtt ccattttgag agactcgaag
    12781 aacagtagct gtaagggcat tcaatttcaa gcctttttca gtccattcaa gtcctttcgg
    12841 gttggcaatt ccgactgatc ctgcttggtt ttcatctgcc aaaacttccg catttcttag
    12901 atgtgcaaag gcagttccca aattaaattc gcgagctttt tcatccgcat tgacaaagac
    12961 tgcgtagata tcgccgtttg gagcagtgat ttggtagcca atcactacat cctctttttc
    13021 cacaccattt tggcctggga cagtgatgag gtggacacgg tctttgatat cttgaagact
    13081 cttaagtcgg aaggcatctg tagattgacg aagggcaatc aaacctttca tatagtcacg
    13141 gctcttgaca ttttcaggat aagctttacc atctgtagcc ttagtccagt caaacttgtt
    13201 gactgcatcg ctagaatcgt aagaatcatg gatgaagtaa ggatagtcaa atgggttgcc
    13261 gtccttatca cgcaacaagt gagatttgtt tggaacctta tcctctgcta ctggagtctt
    13321 gtaggctggg tcacggaatt gtttagtacg tccatattcc tgaccggagt ggataaatgg
    13381 agttccttga gctgtcaaga ccattagatt tccaagtcgt aaacgacggt ggatttcagc
    13441 atagttctca gctttgcttg ggtctttttt gatagactgg gcaatgatgt caaagagggt
    13501 caagttatca tgggctgcga tgtattggat gacatctcca gggctgtcag cttcaaagtt
    13561 agttggctga gcaatgagat ttttaaagat ggtgttgaca tcacgcttgc cacctgtgat
    13621 aaaggcaggt tgaccttcgt ttggataacc agatttgagg ttgttacgga tgtcatctga
    13681 aaagacagcg acagtatcgg tatgtttcat ccaatcttgg tcagcagctt tagtaggcat
    13741 gttttcatca ccggcatagg ttctccaacc ttcgcctaac atgattagat ttggattgag
    13801 ggcgcgtgca gccttgtaag cttcttcgat agaagcggcg tcatggtctc ccatcatatc
    13861 gaaacggaat ccatccactt tgtaggtatc aactaggtat ttgatagagt caactaggag
    13921 ccgtttggtc atatggtggg ttgtccccaa gcgtccacca ccaaagctag ttcgaggtgt
    13981 gccatcggca tccataaagt ggtagtagtt tggctctagg tcttcaaaga tatcgacttt
    14041 ggctgtgtgg ttataaacga catctaggat agctcccata ccacgtttgt ggatttcgtt
    14101 gatgaggttt ttaaattctg cgattcgttt ttctggattc ttaggatcgc ttgagtacat
    14161 accagtcaag gagaagtagt tttgagggtc atatccccag ttgtagttgc tgttgcttga
    14221 agcgtagtca gacaagcgtt catggttttt caattcattg acaaagtagt aagacaagac
    14281 tggaaggagc tggatatggg ttacacccaa gtctttgaga tagtctagtt tttcaatgaa
    14341 ggcttcaaaa gtcccaaatg gtttggtcaa gtcttttgca atggcaggat ctgaagtgaa
    14401 gtcacgcaca tgagcttcgt agataacggc gtcttcacga gtcttgaaat tgtgaatctt
    14461 accataagtc aagtcttgag gtccgagttt agctggatct acaaaggcgg ctttagccac
    14521 tttatgggca tcgtcaattt tggcatcgtc gctattccaa gcagcaagag atttagcgta
    14581 aggatcgagt gcaagaacag ttttaccttg acgctcgatt tggtattgat aatagtagcc
    14641 agtgaaatct gtgattccga gtttgtttgt gctgtctaga gtttgtttcc aagttcctct
    14701 ttcccctttt tcgagagcga cagttccaac tactttgtca gggtcattct tgtcgtagac
    14761 aacaacagaa accttatcag cacttggtga ccaaagggtc aaatcaactt gttttccttc
    14821 ttcttttagg tcagctccca gtttgccatc atagctgtat gtctcatctt tcaggcgcca
    14881 gcttgttttg gtagtgaatt ggtcggaatt gtagctaaca gtataaggat gttttgtgtc
    14941 agagaaatct ccgctgtagg tcactttctt accagcttca tcgattgcaa catcggtaat
    15001 agttaccttg tttcctaggt gattagtgat gttggagtgt ttgaggatat cttctttttt
    15061 agcaccgaca agtgttgaaa agctactttc aatgctagaa gtgcctacgt gttgggctcc
    15121 tgtcatacgg atatcatgga catagtatgg atttgtgtaa atcgattcat catcgtcttt
    15181 taggaaaatt tggctatgat ttttcaaatc tgtgaactta taattttctt tacggatttt
    15241 cacgtcgtct ccttgtttgc tctcatctag taataaaaat ccaaattctc ttgcggcttc
    15301 attaagagga atgtcgatat agcggccata tttgcctgta gccgtaaagt ctgttccgtc
    15361 aggccattga gcgctacttg gattttttac atctccccag taccagagag atttcttgtc
    15421 atagttgcca tctgtgcggt agtagttgac gcgaacagtt cctgcaggct gtggctcgta
    15481 agagaaaacc ttgtaatctt ggtctaacca agcctcgttc atttttggaa ctagtttttc
    15541 tacagattta tcgccggtta gattttttcc agctgtattg ttgatgagga agctaatttt
    15601 cttggcttgt tctcccttta atttgacatc taggtaatag ccgtagtcat ctttcttggc
    15661 atccttgaag gacaaagctc cgtttggcca gttttcagat ggtttttcaa catcgtccca
    15721 agtccatagt ccttgagcat ccttgttttc ttcaggaagt tttttgacat ggatacggaa
    15781 gtagttatct tcgatgggct cttctgcctt agtttcagtt gttactggac tagtagaagt
    15841 agttggctct gttgcaggtt tttcttctgt tttctctagg gaagcgtttg catttctagg
    15901 tgaagaaata tcactttcgt tcttaccaga gccagctgct gtatcagtaa gaggttgggc
    15961 aagggtggtt gtatttctgt tgttgggttc gttagtggtt ggtgtacttt cgtcggatga
    16021 gatagttggt gtagccatgg caagcaagac aaggcttgcg ccgataagga cagaaccagt
    16081 tccatttttg agggaacgga tgctgtagac catttttttc tcagtgtgag atggtgtttt
    16141 tctcataatg attctccaat caataaattt ctatatcagt atagcatgta gtaaaacaat
    16201 atgcaagcgt tttctttgat ttgtgaaaaa aattcttact tttgagaagt tttctgtact
    16261 attttatata tcagtataaa tgacggagat aaaaatgtca cataagccaa gccgtattat
    16321 taggaagatg atgtgttccc agctattttt attcagaaat tgatctctaa cattaccaat
    16381 aaaaaagaaa aatatcttga caagcaaggg aaaattttgt tacaataata gacggtactt
    16441 tttacttttg gtctctcaag agtgtacagg gacgtgctga caaatgttgc aaaagtacac
    16501 acagatgata tctgtcacca agtgtatcat caccaaaaat aaaaaaacac aggagaatgt
    16561 agatgcctac aattaaccaa ttggttcgca aaccgcgtaa atcaaaagta gaaaaatcta
    16621 aatcaccagc tttgaacgtt ggttacaata gtcataaaaa agttcaaaca aacgtttctt
    16681 caccgcaaaa acgtggtgtt gcaactcgtg ttggaacaat gacacctaaa aaacctaact
    16741 cagcccttcg taaattcgct cgtgtacgtt tgagcaacct tatcgaagtt actgcctaca
    16801 tcccaggtat cggacacaac ttgcaagagc acagcgtggt gcttcttcgc ggtggacgtg
    16861 taaaagacct tccaggggta cgttaccata tcgtccgtgg tgcacttgat actgcaggtg
    16921 ttaacgatcg taaacaaggc cgttctaaat acggtactaa acgtccaaaa gcataaggaa
    16981 aggggataaa gagaaatgag tcgtaaaaat agagctccaa aacgtgacgt attgccagat
    17041 ccgctataca attcacaact agttactcgt cttatcaacc gcgttatgct tgatggtaaa
    17101 cgtggtactg ctgcttcaat cgtttacggt gcttttgagc aaatcaaaga agctactggc
    17161 aacgatgcac ttgaagtatt tgaaacagct atggaaaaca tcatgcctgt acttgaagta
    17221 cgtgcacgtc gtgttggtgg ttctaactac caagtcccag ttgaagttcg tccagaacgt
    17281 cgtacaacac ttggacttcg ttggttggta acaatcgctc gtcttcgtgg tgaacacaca
    17341 atgcaagacc gtcttgcaaa agaaatcttg gatgctgcta acaacactgg tgcagcagtt
    17401 aagaaacgtg aagatactca ccgtatggct gaagctaacc gtgcattcgc acacttccgt
    17461 tggtaagata ggatgcgaaa gcgttaagaa agtcccagag aaaataggga atcgaagcag
    17521 gttgcggttg caaccaatga gattcatctt tttctccaga cttttagctt gagctcaact
    17581 aaatcatgat gctaggaacg gtaaggatgc aaggtgaaaa taggaaactg acgcagtatt
    17641 cgacgaatac aaggagtttt atctttttca cgcagcatcc cgttccagct cacatcggct
    17701 aactaacttt agcccgggtt caaattagct aaatcgatta gtattagcta taactcagct
    17761 taccatctcg taagttgaaa ccaacaatag catgaaaaca ttgagaacgg gtaggtcctg
    17821 cctatccgtt tttattaaaa tcgtgttata atagaataga aatcaaaaat aaataggaga
    17881 aacaaacctc atggcacgcg aattttcact tgaaaaaact cgtaatatcg gtatcatggc
    17941 tcacgtcgat gccggtaaaa caacaactac tgagcgtatt ctttactaca ctggtaaaat
    18001 ccacaaaatc ggtgaaactc acgaaggtgc gtcacaaatg gactggatgg agcaagagca
    18061 agaacgtggt atcacgatca catctgctgc gacaacagct caatggaaca accaccgcgt
    18121 aaacatcatc gacacaccag gacacgtgga cttcacaatc gaagtacaac gttctcttcg
    18181 tgtattggat ggtgcggtta ccgttcttga ctcacaatca ggtgttgagc ctcaaactga
    18241 aacagtttgg cgtcaagcaa ctgagtacgg agttccacgt atcgtatttg ccaacaaaat
    18301 ggacaaaatc ggtgctgact tcctttactc tgtaagcaca cttcacgatc gccttcaagc
    18361 aaatgcacac ccaatccaat tgccaatcgg ttctgaagat gacttccgtg gtatcattga
    18421 cttgatcaag atgaaagctg aaatctatac taacgacctt ggtacggata tccttgaaga
    18481 agacatccca gctgaatacc ttgaccaagc tcaagaatac cgtgaaaaat tgattgaagc
    18541 agttgctgaa actgacgaag aattgatgat gaaatacctc gaaggtgaag aaatcactaa
    18601 cgaagaattg aaagctggta tccgtaaagc gactatcaac gttgaattct tcccagtatt
    18661 gtgtggttca gccttcaaaa acaaaggtgt tcaattgatg cttgatgcgg ttatcgacta
    18721 ccttccaagt ccacttgaca tcccagcaat caaaggtatt aacccagata cagacgctga
    18781 agaaactcgt ccagcatctg acgaagagcc atttgcagct cttgccttca agatcatgac
    18841 tgacccattc gtaggtcgtt tgacattctt ccgtgtttac tcaggtgttc ttcaatcagg
    18901 ttcatacgta ttgaatactt ctaaaggtaa acgtgaacgt atcggacgta tccttcaaat
    18961 gcacgctaac agccgtcaag aaatcgacac tgtttactca ggtgatatcg ctgctgccgt
    19021 tggtttgaaa gatactacaa ctggtgactc attgacagat gaaaaagcta aaatcatcct
    19081 tgagtcaatc aacgttccag aaccagttat ccaattgatg gttgagccaa aatctaaagc
    19141 tgaccaagac aagatgggta tcgcccttca aaaattggct gaagaagatc caacattccg
    19201 cgttgaaaca aacgttgaaa ctggtgaaac agttatctca ggtatgggtg aacttcacct
    19261 tgacgtcctt gttgatcgta tgcgtcgtga gttcaaagtt gaagcgaacg taggtgctcc
    19321 tcaagtatct taccgtgaaa cattccgcgc ttctacccaa gcacgtggat tcttcaaacg
    19381 tcagtctggt ggtaaaggtc aattcggtga tgtgtggatt gaatttactc caaacgaaga
    19441 aggtaaagga ttcgaattcg aaaacgcaat cgtcggtggt gtggttcctc gtgaatttat
    19501 cccagcggtt gaaaaaggtt tggtagaatc tatggctaac ggtgttcttg caggttaccc
    19561 aatggttgac gttaaagcta agctttatga tggttcatat cacgatgtcg actcatctga
    19621 aactgccttc aagattgcgg cttcactttc ccttaaagaa gctgctaaat cagcacaacc
    19681 agctatcctt gaaccaatga tgcttgtaac aatcactgtt ccagaagaaa accttggtga
    19741 tgttatgggt cacgtaactg ctcgtcgtgg acgtgtagat ggtatggaag cacacggtaa
    19801 cagccaaatc gttcgtgctt acgttccact tgctgaaatg ttcggttacg caacagttct
    19861 tcgttctgca tctcaaggac gtggtacatt catgatggta tttgaccact acgaagatgt
    19921 acctaagtca gtacaagaag aaattattaa gaaaaataaa ggtgaagact aatccgtcct
    19981 cactctagaa ggaagtcact tagtggcttc cttttgtctt tagaaaatac ctctaaatat
    20041 ggtaaaatag tagaagaata atgtgaggaa aatgaatgtc aaatagtttt gaaattttga
    20101 tgaatcaatt ggggatgcct gctgaaatga gacaggctcc tgctttagca caggccaata
    20161 ttgagcgagt tgtggttcat aaaattagta aggtatggga gtttcatttc gtattttcta
    20221 atattttacc gattgaaatc tttttagaat taaagaaagg tttgagcgaa gaattttcta
    20281 agacaggcaa taaagctgtt tttgaaatta aggctcggtc tcaagaattt tcaaatcagc
    20341 tcttgcagtc ctactatagg gaggctttct ctgaaggtcc atgtgctagt caaggtttta
    20401 agtcccttta tcaaaatttg caagttcgtg ctgagggcaa tcagctattt attgaaggat
    20461 ctgaagcgat tgataaggaa cattttaaga agaatcatct tcctaattta gccaaacaac
    20521 ttgaaaagtt tggttttcca acttttaact gtcaagtcga taagaatgat gtcctgaccc
    20581 aagagcagga agaggccttt catgctgaaa atgagcagat tgttcaagct gccaatgagg
    20641 aagcgctccg tgctatggaa caactggagc agatggcacc tcctccagcg gaagagaaac
    20701 cagtctttga ttttcaagcg aaaaaagctg cagctaaacc caagctggat aaggcggaga
    20761 ttactcctat gatcgaagtg acgacagagg aaaatcgtct ggtatttgaa ggggttgttt
    20821 ttgatgtgga gcaaaaagtg actagaacag gtcgtgtttt aatcaacttt aaaatgacgg
    20881 actacacttc aagtttttct atgcaaaagt gggttaaaaa cgaggaagag gcccagaagt
    20941 ttgatctcat caagaagaat tcttggctcc gagttcgagg gaatgtggag atgaataact
    21001 tcacacgcga tttgactatg aacgtacagg atctgcagga agttgttcac tatgagcgga
    21061 aggatttgat gccagaaggt gagcgtcggg ttgagtttca tgctcatact aacatgtcga
    21121 ctatggatgc tttgccagag gtcgaagaga ttgttgcaac agctgctaag tggggacaca
    21181 aggcggttgc tatcacagac catgggaatg tccagtcctt tccacatggc tataaggcgg
    21241 ctaagaaagc gggaatccag ctgatctatg ggatggaagc caatatcgtg gaggaccgtg
    21301 tccctatcgt ctataacgaa gtggagatgg acttatcaga agcaacctac gtggtctttg
    21361 acgtggaaac gacgggactt tcagctatct ataatgactt gattcaggtt gcggcctcta
    21421 agatgtacaa ggggaatgtt attgctgaat ttgatgaatt tatcaatcct gggcatccct
    21481 tgtcagcttt tactacagag ttaactggaa ttacagatga tcatgtcaaa aatgccaaac
    21541 cactagaaca agttttgcaa gaattccaag aattttgcaa ggatacggtc ctagttgccc
    21601 acaatgctac ctttgacgtt ggctttatga atgctaatta tgagcgtcat gatcttccaa
    21661 agattagtca gccagttatt gatacgctgg agtttgctag aaacctctat cctgagtata
    21721 aacgccatgg tttggggcct ttgaccaagc gttttggtgt ggccttggaa catcaccaca
    21781 tggccaacta cgatgcggaa gcgactggtc gtctgctttt catctttatc aaagaggtag
    21841 cagaaaaaca tggtgtgacc gatttagcta gactcaacat tgatctaatc agtccagatt
    21901 cttacaaaaa agctcggatc aagcatgcga ccatctatgt caagaatcag gtaggtctaa
    21961 aaaatatctt taagctggtt tccttgtcta ataccaagta ttttgaagga gtgtcacgga
    22021 ttccgagaac ggttctagat gcccatcgag agggcttgat tttaggttca gcctgttcag
    22081 agggtgaagt ttttgacgtg gtcgtttctc aaggtgtgga tgcggcggtt gaggtggcca
    22141 agtattatga ctttatcgag gtcatgccac cggctatcta tgcgcccttg attgctaaag
    22201 agcaggtcaa ggatatggag gaactccaga ccattatcaa gagtttgata gaggttggag
    22261 accgccttgg caagcctgtt ttggctacgg gaaatgttca ctatatcgaa ccggaagaag
    22321 agatttatcg tgaaattatc gtccgtagtt tgggacaggg tgcgatgatt aaccgaacta
    22381 tcggtcatgg tgaacatgcc caaccagcac cacttccaaa ggctcatttt cgaacgacta
    22441 atgagatgtt ggatgaattt gcctttttgg gagaggaact ggctcgtaaa ctggttattg
    22501 aaaacaccaa tgccttggca gaaatatttg aacccgttga agtcgttaag ggtgacttgt
    22561 atacgccttt catcgacaag gctgaagaaa cagttgctga gttgacctat aagaaagctt
    22621 ttgagattta tggaaatccg ctgccagata ttgttgattt gcggattgaa aaagaattaa
    22681 catccatact ggggaatgga tttgctgtga tttatctggc atcgcagatg ctggtgcaac
    22741 gttctaatga acggggttat ttggttggtt ctcgtgggtc tgtcggatct agtttcgttg
    22801 cgaccatgat tgggattacg gaggtcaatc ctctctctcc tcactatgtc tgtggtcagt
    22861 gtcagtacag tgagtttatc acagatggtt cgtacggttc aggatttgat atgccccata
    22921 aggactgtcc aaactgtggt cacaaactca gtaaaaacgg acaggatatt ccgtttgaga
    22981 ccttccttgg ttttgatggg gataaggttc ctgatattga cttgaacttc tcgggagaag
    23041 atcagcctag cgcccacttg gatgtgcgtg atatctttgg tgaagaatat gccttccgtg
    23101 cgggaacagt tggtacggta gctgccaaga ctgcctatgg atttgtcaag ggttacgagc
    23161 gagattatgg caagttttat cgtgatgcag aagtagaacg cctcgctcaa ggagcggcgg
    23221 gtgtcaagcg gacaacaggc caacacccgg ggggaatcgt tgttattccg aactacatgg
    23281 atgtctacga ttttacgcct gtccagtatc cagcagatga tgtcacggct gaatggcaga
    23341 ccactcactt taacttccac gatatcgatg agaacgtcct caaactcgat gtactgggac
    23401 atgatgatcc gaccatgatt cgaaaacttc aggatttgtc tggtattgac cctaataaaa
    23461 ttcctatgga tgacgaaggc gtgatggcac tcttttctgg gactgatgtg ctaggggtaa
    23521 cacctgaaca aattggaacg cctacgggta tgttggggat tccagagttt ggaacaaatt
    23581 tcgtacgtgg aatggtagac gaaacccatc cgacaacctt tgcggaattg cttcagctgt
    23641 ctggtctgtc ccacggtact gatgtttggt tggggaatgc tcaggatctg attaagcaag
    23701 gaatagcgga cctatcgact gttatcggtt gtcgggacga catcatggtt tacctcatgc
    23761 atgcgggtct ggaacctaag atggccttta ctattatgga acgggtacgt aagggtttgt
    23821 ggctaaagat ttcagaagag gagagaaatg gctatatcga agcaatgaag gctaataagg
    23881 tgccagagtg gtatatcgaa tcctgtggga aaattaagta catgttccct aaggcccatg
    23941 cggcagccta cgttatgatg gccttgcgtg tagcttactt caaggttcac catcctattt
    24001 attactactg tgcttacttc tccattcgtg ctaaggcttt tgatatcaag accatgggtg
    24061 cgggcttgga ggtcatcaag cgcagaatgg aagaaatctc tgaaaaacgg aagaacaatg
    24121 aagcctctaa tgtggaaatc gatctctata caactcttga gattgtcaat gagatgtggg
    24181 aacgaggttt caagtttggt aaattagatc tctactgtag tcaggcgaca gagttcctca
    24241 tcgacgggga tacccttatc ccaccatttg tagcaatgga tggtctggga gagaacgttg
    24301 ccaagcaact ggtgcgggcg cgtgaagagg gagaattcct ctctaaaaca gaactacgca
    24361 agcgtggtgg actctcatca accttggttg aaaagatgga tgagatgggt attcttggaa
    24421 atatgccaga ggataaccag ttgagtttgt ttgatgagtt gttttaaaaa attgcttaat
    24481 aatctattaa aagaggctaa cgtatatcca atagatttac attagctttc ttttttgtta
    24541 aaatagtcta tggaaagagg gtgagagtat gtcaaagatg agtataagca tccgtctgga
    24601 tagtgaggtt aaggagcagg cccaacaggt gtttagtaat ctgggaatgg atatgacaac
    24661 agctattaat attttccttc gtcaggcaat tcaatatcag ggattacctt ttgatgttag
    24721 actagacgaa aatcggaagt tgctccaagt gttaacggat ttagaccaaa atcgtaatat
    24781 gagccagtct tttgaatcag tctcagattt gatggaggac ttacgtgctt aagattcgtt
    24841 atcataaaca gtttaaaaaa gattttaagt tggctatgaa gcgtggtttg aaggcagaat
    24901 tattagaaga agttttgaat tttctggttc aagaaaaaga acatcctgcc agatatcgtg
    24961 atcattcatt gacggcatcc aagcattttc aaggagttcg tgaatgccat acccagccag
    25021 attggctttt ggtttataaa gtagacaagt cggaattgat tttaaatttg ctgaggacag
    25081 gcagtcacag tgatttattt taatctattt taagggggtt ctcatgaaac taagaatatt
    25141 tgcggaagat aagccggcta agaaggtatt tgaatatcaa ttagaacttg ctgatcgtac
    25201 aattcttcta tcgacagcac tcttgtcagg tgctattgct ttagcaggaa tcttttctgc
    25261 tttgaaagaa aaataaaaat agaaaagaga aaacagaatg gttttaccaa attttaaaga
    25321 aaatctagaa aaatatgcga aattgttggt tgcgaacgga attaacgtgc aacctggtca
    25381 cactttggct ctctctattg atgtggagca acgtgaattg gcacatctaa tcgtgaaaga
    25441 agcttatgcc ttgggtgcgc atgaggtcat cgttcagtgg acagatgatg tgattaaccg
    25501 tgagaaattc ctccatgccc cgatggagcg tttggacaat gtgccagaat acaagattgc
    25561 tgagatgaac tatctcttgg agaacaaggc tagccgtctt ggagttcgtt catctgatcc
    25621 aggtgccttg aacggagtgg atgctgacaa gctatcagct tctgctaaag ctatgggact
    25681 tgccatgaaa ccaatgcgaa tcgcaactca atccaacaag gttagctgga cggtagcagc
    25741 tgctgctgga cttgagtggg ctaagaaagt ctttccaaat gctgcgagcg atgaagaagc
    25801 agtcgatctc ctttgggacc aaattttcaa aacttgccgt gtctatgaag aagatcctgt
    25861 taaggcttgg gaaaatcatg cagctatcct taagagcaag gctgatatgc ttaataaaga
    25921 acaattttca gcccttcact acacagcgcc agggactgat ttgacccttg gtttgccgaa
    25981 gaaccacgtt tgggaatcag ctggtgctat caatgcacag ggcgaaggtt tcttgccaaa
    26041 tatgccgaca gaagaagtct tcacagctcc agacttccgt cgtgcagatg gttatgtaac
    26101 ctctacaaaa ccgcttagtt ataacggaaa cattattgaa ggtattaagg tgacctttaa
    26161 ggatggacaa atcatagata ttactgctga gaagggtgat caggttatga aggatcttgt
    26221 ctttgaaaat gcgggtgcgc gtgccttggg tgaatgtgcc ttggtaccag atccaagccc
    26281 aatttctcag tcaggtatta ccttctttaa cactcttttc gatgaaaatg cgtcaaatca
    26341 cttggctatc ggtgcagcct atgcgactag cgtcgttggt ggagcggaga tgagcgaaga
    26401 ggagcttgaa gctgcagggc ttaaccgttc agatgttcac gtagacttta tgattggttc
    26461 taaccaaatg gatatcgatg gtattcgtga ggatggaacg cgggtacctc ttttccgtaa
    26521 tgggaattgg gcaaattaag gagataatat gttaggaagt atgttcgttg gtctcctagt
    26581 gggattttta gcaggtgcta tgaccaatcg tggagagcga atgggatgtt ttggaaaaat
    26641 gtttctcggt tggatcggag cctttctagg tcacttgctc tttggaactt gggggccagt
    26701 tttatcagga acagctatta tcccagcgat tttaggagcc atgattgttt tagctatttt
    26761 ttggagacga ggaagttaat ttcctaaatc tgatactcaa tgaaaatcaa agagcaaact
    26821 aggaaactag ccgcaggttg ctcaaagcac tgctttgagg ttgcagatag aactgacgaa
    26881 gtcagctaaa aacactgttt tgaggttgca gatagaactg acgaagtcag taacatatat
    26941 acggcaaggc gacgttgacg cggtttgaag agatttttga agagtatgaa acgaaaagga
    27001 ggttggtcat tgtactagcc tccttttgat atgatataat agttctatga gattagataa
    27061 atttttagtt gcctgtgcgg tgggaagtcg gactgaggtc aaaaacttgc tcaaggctgg
    27121 gcgcgtgact gtaaatggta aaaaagaaaa atcagctaaa ttgcagattg atgaaaaaat
    27181 agatgagatt cgctttgatg ggcaagtgtt ggagtatgaa gagtttgtct actacatgat
    27241 gaacaagccc aaaggagtta tctcagcgac tgaggatccc aagcacagaa ccgttctgga
    27301 cttgttggat gacttggcgc ggagtaagga agtttttcca gtaggacgct tggatattga
    27361 cacgcatggt cttttgctct tgaccaataa tggtcagctg gctcatgttc ttctttcgcc
    27421 caagcgtcat gtggacaaga cttatcaggc tcaggtcaag ggaattatga cccaagaaga
    27481 tgtggagaca tttatcaagg gcattccgct caaagacttt acctgtcagc ccgctagact
    27541 ggagattctt tccacagatg cagaaaagaa tcagagccaa atccgtgtga ccattgcaga
    27601 agggaagttt catcagatca agcgtatggt gggctactgt ggcaaggaag tggtggactt
    27661 gcaacgattg actatgggaa ctttagtatt agatgagaac ctggaacgag gggaatggcg
    27721 tcgcttgacc aaggaagaat tagaaattct ccgcgctaat attatttaat ttagtttaat
    27781 actcaatgaa aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggc
    27841 aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag tataaattcg aaaaaggtga
    27901 gggagaatgt ccttaccttt tatgtttttt agttgcttcc tttgtgaaaa gagttataat
    27961 agactgtaga ataaaaggag gaatctatga acgcgattca agaatcattt actgataaac
    28021 tatttgccaa ctatgaagca aatgtaaaat accaagcgat tgaaaatgct gccagccaca
    28081 acggaatttt tgcagctctg gaacgtcgcc aaagccatgt agacaacaca cctgttttct
    28141 cattggattt aaccaaggac aaggtcacta accagaaagc gtctggtcgt tgctggatgt
    28201 ttgcggctct caacaccttc cgccacaaac tcatctcgca atacaaattg gagaactttg
    28261 agttgtcaca agcccacact ttcttctggg ataagtatga gaaatcaaac tggttcttgg
    28321 agcaagtcat tgcgacttca gaccaagaat tgactagccg caaggttagc ttcttactcc
    28381 aaacacctca acaagatggt ggtcaatggg atatggtcgt ttccctcttt gaaaaatacg
    28441 gtgtcgtgcc taagtcagtt tatcctgagt ctgtttcatc tagcagcagt cgtgagctaa
    28501 atgcgatcct taataaattg cttcgtcaag atgctcaaat cttgcgtgat ttgcttgttt
    28561 ctggtgcaga tcaagtgact gttcaagcta agaaagaaga cctcttgcaa gaaatcttta
    28621 actttcttgc tatgtcatta ggacttccac cacgcaagtt tgactttgct tatcgcgata
    28681 aagataacaa ctacaaaagt gaaaaaggaa tcacaccaca agagttctac aagaaatatg
    28741 tcaatcttcc tttagaagac tacgtttctg ttatcaatgc tccaactgct gataaacctt
    28801 acggaaaatc ttacacagtt gagatgttgg ggaatgtggt tggtagccgt gcagttcgct
    28861 acatcaacgt tcctatggag cgcttgaaag agttggcgat tgctcaaatg caagcaggtg
    28921 agactgtttg gtttggttca gatgtcggtc agctcagcaa ccgtaaagct ggaatccttg
    28981 cgacagatgt ttatgacttt gaatcaagca tggacattaa acttactcaa gacaaggctg
    29041 gacgtttgga ctatagtgaa agcttgatga cccacgccat ggtcttgaca ggtgttgact
    29101 tggacgaaaa tggtaaatca accaagtgga aggttgaaaa ctcatgggga gacaaggtcg
    29161 gtacagatgg ttactttgtt gcctcagacg cttggatgga cgaatacaca taccaaatcg
    29221 ttgttcgtaa ggaattgctg acagcagaag aacaagctgc ctatggagca gaaccaatcg
    29281 tacttgcacc atgggatcca atgggatcct tggctgaata aaagcataga aaaaa
//
LOCUS       DAVMHG010000009        28488 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMHG010000009 DAVMHG010000000
VERSION     DAVMHG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.009"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            79..354
                     /locus_tag="VYR08_000307"
     CDS             79..354
                     /locus_tag="VYR08_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465893.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            complement(781..987)
                     /locus_tag="VYR08_000308"
     CDS             complement(781..987)
                     /locus_tag="VYR08_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465894.1"
                     /translation="MTDYQLEASLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVKIDRSNSDQITFLIK"
     gene            1060..1287
                     /locus_tag="VYR08_000309"
     CDS             1060..1287
                     /locus_tag="VYR08_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3465895.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHNLGEV"
     gene            1330..1692
                     /locus_tag="VYR08_000310"
     CDS             1330..1692
                     /locus_tag="VYR08_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3465896.1"
                     /translation="MQYIIRNTHGNYTSLNNAYAQDKRLKATTIGILTVILMNKSDWV
                     VYPDEIARRLGISRRTVDEHFKLLEKAGYLRVYRLGLGRGKGVTVYRFFSDMPISDDY
                     FEYLKTNLEKELSTDNGV"
     gene            1927..2108
                     /locus_tag="VYR08_000311"
                     /pseudo
     CDS             1927..2108
                     /locus_tag="VYR08_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2105..2329)
                     /locus_tag="VYR08_000312"
     CDS             complement(2105..2329)
                     /locus_tag="VYR08_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEV3465897.1"
                     /translation="MGKNQHVVPAKNGGWNVKGAGNSRATVHTTTKNEAKNIARQISR
                     NQGSELIIHGKDGKIQSRDSHGKDPFPPKG"
     gene            2446..2589
                     /locus_tag="VYR08_000313"
     CDS             2446..2589
                     /locus_tag="VYR08_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465898.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            2656..3453
                     /locus_tag="VYR08_000314"
     CDS             2656..3453
                     /locus_tag="VYR08_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV3465899.1"
                     /translation="MNMNLNTNEILTTTEYDMFRKISNRKITENPKLEEELLSEGQRQ
                     PILVNEKMEVIDGQHRLYYLRKHRKPVRYIIDPTANFKTVISMNTSAVNWALQDYVYS
                     FALEGDPEFVKLAKFLDENELLSDKMVIVAGSGRRDGTAAHVVKKLKKGDYVFSNEKQ
                     LREFCKFYERVLNETKLPNKPFLQSVLWTLYTTSVFDENRMLTQLKKSDLTSEDIEGF
                     AKKKLLLTFLELYNGRWSDDHPSLIQYFINRKGSLTIPSLPKQDEDN"
     gene            3471..3608
                     /locus_tag="VYR08_000315"
     CDS             3471..3608
                     /locus_tag="VYR08_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465900.1"
                     /translation="MKVTVYVYGRKLEPDEPIIIPENHRFYDIWNGIVNEMLDNEEDV
                     A"
     gene            3608..3811
                     /locus_tag="VYR08_000316"
     CDS             3608..3811
                     /locus_tag="VYR08_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465901.1"
                     /translation="MKLLTKLKLRLEGVLKSVNLDWREVAVELMNDLFEERKRRFAFE
                     QENYDLKQQLAIYKEKEQGEQYV"
     gene            3804..4058
                     /locus_tag="VYR08_000317"
     CDS             3804..4058
                     /locus_tag="VYR08_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465902.1"
                     /translation="MFKALKTIKKIKQLQKEMHDASVAFLLMQDLGLVPDSEKGRTIA
                     KSFYDVSHMLKDVLGGKSVDEAMKRLNSEVKIEDVEQEDD"
     gene            4363..4548
                     /locus_tag="VYR08_000318"
     CDS             4363..4548
                     /locus_tag="VYR08_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465903.1"
                     /translation="MKKRLYYKKWKQELREAMREEIDGDYLTEKMVRKMSISDMSHYL
                     NQLALEEAGYCGTMFNY"
     gene            4566..5318
                     /gene="bet"
                     /locus_tag="VYR08_000319"
     CDS             4566..5318
                     /gene="bet"
                     /locus_tag="VYR08_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV3465904.1"
                     /translation="MTNNQLATQTKRNITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYTNRNGGKEFSLIVSKEAFLKRAAQCKDYEGFEAGVVV
                     VDSEGVMHERKGAIMLPEDILIGGWARVHRKNFKVPVEIFVSREEYDKKQSTWNTMPA
                     TMIRKVALVNALREAFPEDLGNMYTEDDGGETFDRIKDVTPQESREDVVARKMAEIEQ
                     FNKEQEANHADPEPAQTEETIQGELLDGELEY"
     gene            5328..6392
                     /locus_tag="VYR08_000320"
     CDS             5328..6392
                     /locus_tag="VYR08_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV3465905.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTAGTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTIAEVFGQ
                     IKADYEFEKQKEEIRQAQERAERESQELLAAQQTKQQEQAPKSTETPNFDPETGKILD
                     GGQILQNEPNALRGAENDLKRYAQKMTLEVYFVDTAEKDCFKAGLSQLGFDFKKNYQV
                     RGYQRIEPLTQAELNEQCGW"
     gene            6395..6556
                     /locus_tag="VYR08_000321"
     CDS             6395..6556
                     /locus_tag="VYR08_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465906.1"
                     /translation="MTEIEKISEELAEYGVPDELIGKIEDLLATLYGEKKEIGDRKIL
                     DISPESMGR"
     gene            6559..6882
                     /locus_tag="VYR08_000322"
     CDS             6559..6882
                     /locus_tag="VYR08_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465907.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFNVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            6875..7210
                     /locus_tag="VYR08_000323"
     CDS             6875..7210
                     /locus_tag="VYR08_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV3465908.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            7305..7721
                     /gene="ssb"
                     /locus_tag="VYR08_000324"
     CDS             7305..7721
                     /gene="ssb"
                     /locus_tag="VYR08_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV3465909.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            7731..8237
                     /locus_tag="VYR08_000325"
     CDS             7731..8237
                     /locus_tag="VYR08_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3465910.1"
                     /translation="MSKIKILDACCGSRMFWFDKNESHTIFMDIRQETFEIHDKKVNV
                     DPDIIGDFRDMSFEDNTFNLVVFDPPHLKWAGPNSIMKAQYGQLDKVTWSEDLAKGFE
                     ECLRVLKVGGTLVFKWSDRQINVKKLLEVIPFKPLFGQQRGTTHWLTFVKFEEDKNGV
                     DGLGGLET"
     gene            8203..8412
                     /locus_tag="VYR08_000326"
     CDS             8203..8412
                     /locus_tag="VYR08_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465911.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     MDIKQDCLRLGVPFEDMYPLQTTLF"
     gene            8426..8755
                     /locus_tag="VYR08_000327"
     CDS             8426..8755
                     /locus_tag="VYR08_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465912.1"
                     /translation="MASKINVTERIAIIIEKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLIRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            8752..9183
                     /locus_tag="VYR08_000328"
     CDS             8752..9183
                     /locus_tag="VYR08_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV3465913.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQTLLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            9199..9381
                     /locus_tag="VYR08_000329"
     CDS             9199..9381
                     /locus_tag="VYR08_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465914.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            9406..9723
                     /locus_tag="VYR08_000330"
     CDS             9406..9723
                     /locus_tag="VYR08_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465915.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     RGN"
     gene            9725..10420
                     /locus_tag="VYR08_000331"
     CDS             9725..10420
                     /locus_tag="VYR08_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV3465916.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLDESEIGH
                     ADEAPRYVKNILARLRELPLHDREVWLKAIMSEFEQDFSRAKWREGYEQGKIEGMVER
                     EKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDGY
                     EVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDAKFGWV
                     FDCPGIEIEEVIE"
     gene            10417..10677
                     /locus_tag="VYR08_000332"
     CDS             10417..10677
                     /locus_tag="VYR08_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV3465917.1"
                     /translation="MKRFIAIWIVLSATLNIWQCVHIKNLEKKRPIVIYKADNKGAEI
                     KGRVVHKEKIGELYTITIQNYGTFVVSQDNYEFLKIGDEVRL"
     gene            10719..11087
                     /locus_tag="VYR08_000333"
     CDS             10719..11087
                     /locus_tag="VYR08_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465918.1"
                     /translation="MYQPDEVMVGDGNIWIIDEDSVAGDWIVNNDIHLMQSTGLFDRN
                     GKEIFEGDIVKRYRSPFFKAEWEYRIEIVIKEKASLLLGRRFDKALCARSLDSPFTKS
                     DLLEVIGNIYENPEVLEVTE"
     gene            11084..11326
                     /locus_tag="VYR08_000334"
     CDS             11084..11326
                     /locus_tag="VYR08_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEV3465919.1"
                     /translation="MNPEKIDNVNKPSHYQGRFGMESIDALRNFMTDEQLKGFYLGNA
                     LKYLLRHQKKNGLEDLKKARKNLDWLIEEMENKNET"
     gene            11316..11576
                     /locus_tag="VYR08_000335"
     CDS             11316..11576
                     /locus_tag="VYR08_000335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465920.1"
                     /translation="MRPKKYPYTGSKINKVTTTGIGAQELVVFPNVAFRKDLLKHIFS
                     VVKQHDNATIIYFRIPKVFGYEEERAKVYLSYEKTMMILNSY"
     gene            11652..12074
                     /locus_tag="VYR08_000336"
     CDS             11652..12074
                     /locus_tag="VYR08_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV3465921.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRLINKLKNPKSRSILRVTYITK
                     MYVDDICDKMEISRTTFYTWRNMAISELNEVLERMELN"
     gene            12529..13029
                     /locus_tag="VYR08_000337"
     CDS             12529..13029
                     /locus_tag="VYR08_000337"
                     /inference="COORDINATES: protein motif:HMM:NF015550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV3465922.1"
                     /translation="MVKKNRNKRSREQVDEFGLNSRERNFADEYIANGNNATQAYLKI
                     YPTSGEANAANKGSAWVRKGEILNYIKIKTKERLDASGLKANDIIDRLIDIAFGRPIV
                     GYSKQTDKITGEVIKYIEYEGTAPIDEQIKALELLGKYLKLFTDKVEADVSGTVVFAN
                     ESDIPD"
     gene            13007..14305
                     /locus_tag="VYR08_000338"
     CDS             13007..14305
                     /locus_tag="VYR08_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV3465923.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDGVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            14317..15786
                     /locus_tag="VYR08_000339"
     CDS             14317..15786
                     /locus_tag="VYR08_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV3465924.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            15695..17332
                     /locus_tag="VYR08_000340"
     CDS             15695..17332
                     /locus_tag="VYR08_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV3465925.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFAAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKTELVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            17334..17543
                     /locus_tag="VYR08_000341"
     CDS             17334..17543
                     /locus_tag="VYR08_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465926.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            17540..17953
                     /locus_tag="VYR08_000342"
     CDS             17540..17953
                     /locus_tag="VYR08_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV3465927.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            17996..18259
                     /locus_tag="VYR08_000343"
     CDS             17996..18259
                     /locus_tag="VYR08_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465928.1"
                     /translation="MKYRKKPVVVEAVQFLDTEESIDELCDFGLDPVRIDYADLKNPL
                     LKIETLEGLMVATEGDYIIKGVQGEYYPCKPDIFAETYEKTEE"
     gene            18261..18512
                     /locus_tag="VYR08_000344"
     CDS             18261..18512
                     /locus_tag="VYR08_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465929.1"
                     /translation="MLEKAKQLASQEFLRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGDYAEITHNGDKNETYVDVYAKVSNRAIKD"
     gene            18739..19323
                     /locus_tag="VYR08_000345"
     CDS             18739..19323
                     /locus_tag="VYR08_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV3465930.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            19329..20171
                     /locus_tag="VYR08_000346"
     CDS             19329..20171
                     /locus_tag="VYR08_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEV3465931.1"
                     /translation="MTAGQTKLATMVNPEVMADMVSAKLPKLIKFTPLAYVETALQGQ
                     PGNTLTVPAWEYAGDATEVGEGQAISPDQLTTKKTTMTIKKAAKGYEITDEALLSGLG
                     DPLGQATYQLGLAIANKIDDDLVAVAKTATQHITETPTTLAAIDKALEIFEDEEDAQY
                     VAIINPKDAIKLKTDVAKEWTKGSELGADMVVSGTFGEVAGVQIVRSKKVDEGKGFIV
                     KVSPSQTQTDDANKYGAFVIMLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGE"
     gene            20183..20365
                     /locus_tag="VYR08_000347"
     CDS             20183..20365
                     /locus_tag="VYR08_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV3465932.1"
                     /translation="MSLLLRRHYIQEEQVSQYSDLENKTLEELKNLAKEAGIAGAYKL
                     SKAEIVEVLEDLKSEI"
     gene            20355..20543
                     /locus_tag="VYR08_000348"
     CDS             20355..20543
                     /locus_tag="VYR08_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465933.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGGELEITEERYVELADNIASNGVA
                     ISDVLEKILPEPEFLEED"
     gene            20545..20856
                     /locus_tag="VYR08_000349"
     CDS             20545..20856
                     /locus_tag="VYR08_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV3465934.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGERSRTEGGIEVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            20837..21175
                     /locus_tag="VYR08_000350"
     CDS             20837..21175
                     /locus_tag="VYR08_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465935.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETIRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            21150..21665
                     /locus_tag="VYR08_000351"
     CDS             21150..21665
                     /locus_tag="VYR08_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV3465936.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            21662..22030
                     /locus_tag="VYR08_000352"
     CDS             21662..22030
                     /locus_tag="VYR08_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465937.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWENFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            22033..22446
                     /locus_tag="VYR08_000353"
     CDS             22033..22446
                     /locus_tag="VYR08_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV3465938.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIEPEKVENTTLEDK
                     VKKYEFGIGDAGELEYKFSYKNDSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFTDGVGG"
     gene            22451..22831
                     /locus_tag="VYR08_000354"
     CDS             22451..22831
                     /locus_tag="VYR08_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465939.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKNKKTSKKKMTVVE"
     gene            22870..23220
                     /locus_tag="VYR08_000355"
     CDS             22870..23220
                     /locus_tag="VYR08_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465940.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKTQERKEKIIDSYR
                     LSQMISNHVSLLLSNDAKIVEFWEYAPELFVEEQQAVELERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            23207..26860
                     /locus_tag="VYR08_000356"
     CDS             23207..26860
                     /locus_tag="VYR08_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465941.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDVAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKFVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPTINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSSIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAKFAHSVATGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWADVTNALSSVSNWFGE
                     MFTNAYNAVKDAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            26869..27219
                     /locus_tag="VYR08_000357"
     CDS             26869..27219
                     /locus_tag="VYR08_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465942.1"
                     /translation="MAQLTINGVAVKPPKYFQVGIQDIDGETGRNANGDMMRDRITTK
                     RKLDCEWGMMTQGELSQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            27224..>28488
                     /locus_tag="VYR08_000358"
     CDS             27224..>28488
                     /locus_tag="VYR08_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV3465943.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGVEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKETKRKAEEALR"
BASE COUNT         9489 a         4894 c         6635 g         7470 t
ORIGIN      
        1 cattgacata cgatttaaat cgtagtataa tataatcaag cttaaggaaa taacaaaaac
       61 aaaccggagg gaaacaccat gaacacatta aacgagaaag caatcaacat ctttaaagca
      121 gtagctaagg aaactttaat ccaaggcact tacgaggaaa acttccttta cagccagctt
      181 gaagcattct gtactaactg ccgtcaattc gcttttggat ggacagagtt agcagaggag
      241 attgaacgcc aagagcgtta ccttctcgat tctggtttca ctcaagagga aatcgatgac
      301 attcgttttg atgcagcatt tgcaggaatg cttgataaat taaatgtagc ctgattggta
      361 tcaccaaggt tcgaatcctt ggcaggttgt tgctcataga gcgaaaaaag agagaaagga
      421 gtagaacgat gaatgaatct tttcttacga tactaggcat atcaatgatt gctagtttta
      481 tcacgaagcc gaccagttga aactggacga ctcagatgta gaaatcattt ctaaaagact
      541 aggacggtct atctaaagaa agggtgaaac aaatggcaac taacagaact atatcagtaa
      601 atacatcaga gcatgatgta ttgttgacgg caagaaaaaa ccaccctgct gtattcgtcg
      661 atggaatgtt tctcgacgga gttgagcgag tggaatttac caatcattat ctggagaagt
      721 gtgaagttgt tttaacgttt aacgatagag ttgaaaccaa tcccttccct ctaaacgata
      781 ttactttatt agaaaagtta tttggtcaga gttcgaacgg tcaatcttta cgggatattg
      841 tcgtgcaaac tcttgaagat gctgattagc atctaaacca tcaaaaaaag aaacatgaac
      901 actaaaactt tcttttccgt ttttcttggt tctatcaaat tctttgccaa ggacaattag
      961 agaagcttct aattgataat cagtcataac atcacctcct ttctagcttt attatagcag
     1021 aattgcgagg aacaaataga aaaataagga ggtaggaacg tgccgaaaat gacattgaga
     1081 gcaataagaa caaattataa cttatctgca aaagaagttg ccgataaact taacattcat
     1141 caacaaacac tgttgaagta tgagcatgat agttcaaaaa ttccaatgga tcttttagat
     1201 aaacttgctc gactatacaa tgttgaaaag gattttattt ttttaggcaa aaaatacgaa
     1261 ttaaatcata atctaggaga ggtgtgaatg aacgagcgag agttatagaa aggggattaa
     1321 atatgaggta tgcagtatat aatcaggaat actcatggga attacacatc tttgaataat
     1381 gcttatgctc aagacaaacg tttaaaggca acaacgatag gtatccttac agtaatcttg
     1441 atgaataagt ctgattgggt tgtgtatcct gacgagattg cacgacgtct aggaataagc
     1501 aggcgcaccg tagatgagca ctttaagctt ttagagaaag caggctatct cagagtatat
     1561 cgcttagggt taggcagagg taaaggcgta acggtatata gatttttttc agatatgcct
     1621 atttcagatg attactttga gtatctaaaa actaatcttg agaaagagtt atccacagat
     1681 aacggagttt aaaaatacag ttggaaaata ttgccatgtg taaaattgcc atgtgtaaaa
     1741 ttgccatgtg taaaattgcc ctctaataag tactaactat acaacaagta ctaactatac
     1801 aataatctaa gcctaacggc actaacttag taataactac taacttacaa caaactaata
     1861 cttctctaaa aaataaaaga gagaaatttc aattttagga ctttgaaaaa taggaaagga
     1921 gaaaaaatga gaccaagaaa atatccgtat agtttcaaac caaatctgat gaacatttta
     1981 gatagtcgtt tctatacacg gctaattgtt gaaacagagg atggagcgaa aaaatagcag
     2041 aagtcacact agatgatgta acttctgcta cagggtatgt tgtaaggcta agaccgaatt
     2101 atgactagcc tttaggaggg aatgggtctt taccgtggct gtcacggctt tgaattttcc
     2161 catctttgcc atgaatgatt agttcggaac cttgatttcg tgaaatctgt ctagcaatat
     2221 ttttagcttc attctttgta gtagtatgaa cagttgctct tgaattgcca gcacctttca
     2281 cgttccaacc accatttttg gcagggacaa catgctgatt tttgcccatg attaaatctc
     2341 ctttctattg gaattttgac taaaacggtg agaggtccta gtcaagagta ttatagcaat
     2401 ttaggaggat attacatcag tcttgaggct gatataggag gttgaatgga agataaaatt
     2461 atcgaacttg ctgactactt catcagcgaa tctacaacgt acagagaagc taaaatagcg
     2521 tgtgagaagc tattgaaaca agtcagccat gagatagaac tcagggcaat ggaaagtaag
     2581 acagtctaga agacaacaaa aagcacctga cggcaatcag gcgcatacta aaaaacttta
     2641 caagaggatt ataacatgaa tatgaactta aatacaaatg aaattttaac aactactgaa
     2701 tatgatatgt ttcgcaaaat tagtaataga aaaataactg aaaatcctaa attggaagag
     2761 gagctacttt ctgagggaca acgtcagccg attttggtaa atgaaaaaat ggaagttatc
     2821 gacgggcaac atcgtcttta ttacttgaga aaacatagaa aaccagtgcg ctacataatt
     2881 gatccgaccg ctaatttcaa gacagtaatt tcgatgaata catcagctgt caactgggca
     2941 ttacaagatt atgtgtattc gttcgcttta gagggagatc ctgaatttgt taaattagct
     3001 aaatttttag acgagaacga attgcttagt gacaagatgg taatcgtagc tggttcagga
     3061 agacgtgatg gtacggcagc acacgtagtc aaaaaattaa aaaaaggcga ttatgtattt
     3121 tcaaacgaaa aacagttaag agaattttgt aagttctacg aacgcgtttt aaacgaaaca
     3181 aaactgccta acaaaccatt tttacaatct gttttatgga ctttgtatac aacatcagtt
     3241 tttgatgaaa acagaatgtt gacacaattg aagaaatcag atttgacatc agaagatatc
     3301 gaaggatttg caaagaaaaa attgctatta acttttttag agctatacaa cggaagatgg
     3361 agtgacgatc atccttcttt aattcaatac tttatcaaca gaaaagggtc gttaacaatt
     3421 cctagtttgc ctaaacaaga tgaagataat taaaaaagta ggaagataaa atgaaagtca
     3481 cagtatatgt ttacggccga aaattagaac cagatgaacc aattatcatc ccagaaaatc
     3541 atcgttttta tgacatttgg aacggaattg taaatgaaat gctcgacaac gaggaggatg
     3601 tagcttaatg aaactactta ctaaattaaa actcagactc gaaggagttc ttaaatcagt
     3661 caaccttgac tggagagagg tagcagtcga acttatgaac gacctatttg aggagcgcaa
     3721 gcgtcgcttt gctttcgagc aagaaaacta tgacttgaag caacaattgg caatctacaa
     3781 agaaaaagaa caaggagaac aatatgttta aagcactaaa aacaatcaaa aaaatcaaac
     3841 agcttcagaa agaaatgcac gacgcaagcg tagcattcct acttatgcaa gacctcggtt
     3901 tggttcctga tagcgaaaaa ggaagaacca tagctaaatc gttttatgat gtaagccaca
     3961 tgctcaagga cgtcctgggc ggcaagtcgg tagatgaagc catgaaacgt ctaaatagcg
     4021 aagtgaaaat tgaagatgtg gagcaggaag atgactagaa ttgaacttga aaaccgtgta
     4081 tggcttttgg ctaatcatga agaaaaaaac gaattgctgg atcttgggct aacatccaag
     4141 gctagatatg tgaagcgagt tctggaacta ggaaaggtgt atgctcatgt ttgattacga
     4201 cagagacata atgcaaccgc ctgaagaacg agaagaacgt gacccagctg attggatttt
     4261 cagcgctggt caatggatct atgtaggaga ttgttagcct atgaatagag agcatttaga
     4321 gcgtgaaaat tggaatttga agaagacgaa aggtagaaag aaatgaaaaa acgattatat
     4381 tacaaaaaat ggaaacaaga acttagagag gcaatgagag aagaaattga tggcgattat
     4441 ctaaccgaga aaatggttag aaaaatgagt attagcgata tgtcacacta tttgaaccaa
     4501 ttagcattag aagaagctgg atactgtggg acaatgttta attactaaaa gaaaaaggag
     4561 agaaaatgac taataatcaa ttagcaacac agacaaaacg taacatcact actgacccaa
     4621 gtttattgac cggggcagac atcaaaaagt attttgaccc acaaaaccta ctgactgaaa
     4681 aacaagtagg tcaggcgcta gccttgtgta aaggtcgcaa tcttaaccca tttgctaacg
     4741 aggtctacat tgtagcttac acaaaccgta atgggggcaa agagtttagc ttgattgtct
     4801 ctaaagaggc tttcttgaaa cgtgccgccc aatgtaaaga ctatgagggc tttgaggctg
     4861 gagtagtggt agtagacagt gagggtgtta tgcacgaacg caaaggggca atcatgctcc
     4921 cagaagatat actgataggc ggatgggcta gagtgcaccg caaaaatttc aaggtacctg
     4981 tggaaatttt tgtcagtcgt gaggaatatg acaagaaaca aagtacatgg aacaccatgc
     5041 cagctaccat gatcaggaaa gtggctctag taaatgctct tagagaggct ttccctgagg
     5101 acttggggaa catgtacaca gaggacgacg gtggagagac atttgaccgt atcaaagacg
     5161 tcacacctca agagagccgt gaggatgtcg tagcacgcaa gatggctgaa attgagcaat
     5221 tcaacaaaga gcaagaggca aatcatgcag atcccgaacc tgctcaaact gaggaaacaa
     5281 tccagggcga attgctagac ggtgaacttg aatattagga ggacaacatg caagaattac
     5341 aggttattga tgataagaaa atcaataaaa tctatgagat gatcacaacg gatgaactta
     5401 ctagagagtc ttttgaaaaa gacctcatag aggctactga gaagtacaag gactatattc
     5461 ctacagctgg aactctcaaa gacgacaagg caaagcgggc tgaatttaac aagttaattg
     5521 agtctaaaaa tcgtatccgt attgacacta aaaacttact atcagagaca gctaacacat
     5581 gggatagtta tgctaagtca attattgacc catttgcaac cgtagttagt gaatttgaca
     5641 aaggtatcaa ggaaatcgaa gaacatcaaa gacaactaaa aatagatacg gttaagagtt
     5701 acctagccaa caaatcagct gagtacatgc tggatcctcg tctctttgat gaaaaggccc
     5761 ttgagtatgt caaagctagc gattttatgg cagatggcgt gacgcttaaa aaagccacta
     5821 tgaagtcact tgatgacatg gtcacatttg aatttcagaa acaacaagaa tttgaaaaag
     5881 ccaagtcagc tatttcaggg ttatgtgctg agtatggcat gactgactca ccttacatta
     5941 gacagctgaa agacttgact attgctgaag tctttggaca aatcaaagct gattatgagt
     6001 ttgaaaagca aaaggaagaa atcagacagg ctcaagaacg agcagagcga gaaagtcagg
     6061 aacttttagc agcccaacaa accaaacagc aagaacaggc tccaaaatca acggaaaccc
     6121 caaattttga cccagaaacg ggcaaaatct tggacggtgg acaaatcctc caaaatgagc
     6181 ctaacgctct tagaggggct gaaaatgacc taaaacgata tgcccaaaaa atgactttag
     6241 aggtgtattt tgtagacaca gccgaaaaag actgtttcaa ggctggtcta agtcaactcg
     6301 gatttgattt taaaaagaac tatcaagtca ggggttatca acgtatcgag ccattaactc
     6361 aggctgaact aaatgaacaa tgtgggtggt aagtatgaca gaaattgaaa aaatttcaga
     6421 agaattggct gaatatgggg tgcctgatga gttaatagga aaaatagagg acctattagc
     6481 gactctgtat ggcgaaaaaa aggaaattgg agatagaaaa atcttggata tttctccaga
     6541 gtcaatgggg aggtaaccat ggacatcaga aaaatatctg acagcgtagc catctactcg
     6601 gacggcaaga aattgcaggt tatccacaac ctaggggatg agtttatcct tgatttcaat
     6661 gtgggagagg atagcgtctg gaacctcaat ggccaagtcg tagaaattat tgacatgatt
     6721 gagcctgtct ttaaagtttt cagcttttgc tcaaaatctg gagagggtat gcaacgctta
     6781 aaacatgcta tcgtccactt tgaaatattt gagcagtaca tcagagataa tcaggaagac
     6841 ctgatgatct ggtggcacaa tccaggaggg gaatatgatt aaaaccgtat ttttttcatg
     6901 tgattatcca catcatgagg cgattgacga ccaaataaat agctggcttg ccgaaaatcc
     6961 aggcattaag ttgattgaca tcaaatttca atcaaatgtg tctgctgtcg ctgacagtgg
     7021 agtcagtgct gaatattggc atacatccgc attgattatt tacaaagttc cctcagagaa
     7081 caatataagc agtattaatt caaatggttt aggtttcata atcagctgtg agaaatgtgg
     7141 tagcttatca ataatcaagg gaaaagatgt aggtcaaaat gtatgttatg aatgcaaagg
     7201 agagaaataa tgaatgattt tatcaaagag attgggatgg ctatcctatg gatgttttta
     7261 gggtatctct tgggagagcg tagcactaga gggggacaaa tcagatgatc aataacgtca
     7321 cactggttgg gaggcttgta gcgcctcctg atctacgaaa aacgcctaat aatgtatcta
     7381 gtttacaggg cacgcttgca gtcaatcgca atttcaaaaa cgaaaatgga gagcgtgagg
     7441 ctgattttat caattttcaa gcttggagag gtacagctga catcattgct cagtattgca
     7501 gcaagggctc acttattggc ctcacagggc gcttacaagt taggtcttac gagaaagacg
     7561 gtcagcgtcg atatgtgact gaagtaatcg ctgagagtgt agctctgcta gagagtcgca
     7621 acagtcagca cggacaaggc aacagtttcc aaaatgggaa tagctcacct tttaccgatc
     7681 ctaacccctt tgacctccca aatgacggtt tgccgtttta ggaggtatat atgtcaaaaa
     7741 ttaaaattct tgacgcttgc tgtggcagtc gtatgttttg gtttgataaa aacgaaagtc
     7801 acacaatttt tatggacatt aggcaagaaa catttgagat acatgacaaa aaggtcaatg
     7861 tagaccctga tattatcggt gattttcgtg acatgtcttt tgaagacaac acatttaatc
     7921 tagttgtgtt tgatccacca catctaaaat gggctggacc taattcgata atgaaagctc
     7981 agtatggaca actggacaaa gttacctggt cggaagattt ggccaagggt tttgaagaat
     8041 gtctgagagt tctaaaagtt ggcggcacac tagtctttaa atggtctgac cgtcagataa
     8101 atgtaaagaa attactagag gtgataccat tcaagccctt atttggtcag caaagaggca
     8161 ccacacactg gctaacgttt gtaaagtttg aggaggacaa gaatggagtg gacggattgg
     8221 gtggattgga aacctgaaac caaaacggac attaaaacca aaattgaaaa tgacgggtac
     8281 acttttccac attacgacaa gaaaaacaat ggcgtcaagt atgtgatttc tacaatggac
     8341 atcaaacaag actgtctaag acttggagta ccgtttgaag atatgtaccc tttgcaaacg
     8401 acactttttt aacaggagaa agaacatggc aagtaaaatc aatgtgacag aacgtattgc
     8461 tatcatcatt gagaaacaaa aaatagaggt cgttacgact ctaaactatg atatgagcat
     8521 tagctttgat aacaaagaca cggctcctac actagatgac aatggtgacc tttttgaacc
     8581 ggtctacaag tgcaaagtta aggcaattcc caaaaatgat gtatttttca cctcattaat
     8641 acgggtcaag agcaatatca agacgttaca agaggttaaa aaattctttg agttcgtaaa
     8701 cgaaaacaga gaaaatctct ttgagatggc aggatttaag ggggctcttg aatgaaattg
     8761 accctgaaca ttgagcctaa acctcaatca cggccaaggt ttgcaagacg tgggagtttt
     8821 accacaactt acgaagacaa ggatatgaaa acatggcgca atcattgcca gctgctcatt
     8881 gctaatcagt acatgggtca gcctatcctt gagggagctt tgagggcacg gcttagattt
     8941 tatatcaaac ctcctcagta catttctaaa gtcaagaaga accaacagac cctcctggat
     9001 gaaattatac cagtaggcaa aaagcctgac atagataact acgaaaaagc gctatatgat
     9061 agcatgtcag ggatcgtctt ccaggacgac ggtcagatag cgctacatga tgtaggcaag
     9121 ttctacagtc taaatccacg gatagaggtt gagattgagg tcatgaaatc cctgagtatt
     9181 tgaagaaatg aggagcagat ggctgactac gcattatatc aaggtgatgt gtttgttacg
     9241 cttggaacat tagcgcagat cagtagcgag acaggaatta ctgaaaggat gttaaagtat
     9301 tacacttaca catcacacca aagacgacac ccaaacggta gggccgttat taaaattgag
     9361 gaggaagata atgagaatta agacggaaag cggaggagtt ggaagatgat ggaagagtta
     9421 aagcaaaaag ttaatgaagt atacaactgg acggtagaag acgggaagcc gcaacctccc
     9481 aagcaagatt taccacaagc ggtgaaagac cgggcggact atttttggga aatggcagaa
     9541 gatggtatga cgtttatggg agcgatggaa tgcatcttcg ctgatgaaaa gcctacagac
     9601 tatgatttgg gagctactaa ggattggttg ccaaaatcta aggagtttga tgattgggtt
     9661 ggctattcgc caggaatgtc tcaggtagtt attgcagttt atttgattta tagaggaaac
     9721 taagatgaat aagcaggaat tgattaaaaa attagaggaa cgaagaacaa taactgggaa
     9781 ttttcaaggt tatgtagttt ggtggaagga tgtaaaagaa atctttgaac aactagacga
     9841 atccgaaata ggtcacgcag atgaagctcc acgctacgta aagaacatac tagcacgatt
     9901 gcgagaattg ccattgcatg atagagaggt ttggttaaag gctatcatga gcgaatttga
     9961 acaggatttt agccgtgcaa aatggcgaga gggctacgag caaggtaaaa ttgagggtat
    10021 ggttgaacgt gaaaaagtca tagttccgca gtgtgtggcg gaatatatag aatttaaaaa
    10081 gaaaaacaat tttcatgttt acggtgcaat gagagtaatt gaagatcatt atgataagaa
    10141 agttcctgat tggttttacg aaaataacat cgaaaaattc tgtcttgctt ggcttgacgg
    10201 ctacgaggtt gaaaaagaga agcggtattt tgttaagatt aaagggaata ttaaagaaaa
    10261 tatgttggtt tatggagaac ttttgaaaag gtatttcttt acaaaaagct ttagtttaga
    10321 cgatgttata tattcccaca cccgtaaaga actagaagac gcaaagttcg gctgggtgtt
    10381 tgattgtcca gggattgaga ttgaggaggt tatagaatga aacgcttcat agcaatctgg
    10441 attgtcttgt cagctacttt gaatatctgg caatgtgtcc atattaaaaa tcttgaaaaa
    10501 aagcgcccga ttgtcatcta taaagctgat aacaaaggcg cagaaatcaa aggcagagtc
    10561 gtccacaaag aaaagattgg cgaactctac acgatcacaa tacaaaatta cggcacattc
    10621 gtggtgtcac aagataacta cgaatttttg aaaattggag atgaggtgag attatgatac
    10681 cgaagtacag agcgtatgat agtggctcat taagtcgtat gtatcaacca gacgaagtga
    10741 tggtcggtga tggcaatatc tggattattg atgaggattc agttgctggt gactggatag
    10801 taaataatga cattcacctc atgcaatcaa caggcctctt tgacagaaat ggcaaggaaa
    10861 tctttgaggg ggatatagta aaacgatata gaagtccttt ttttaaagca gaatgggaat
    10921 atcggattga aattgtaatc aaagaaaaag ccagtctttt gttaggtcga agatttgaca
    10981 aggccctttg tgcaagatca ctcgactcgc cgtttactaa aagtgatctg ttggaagtca
    11041 tcggcaatat ctatgaaaat ccggaagttt tggaggttac tgaatgaacc cagaaaaaat
    11101 tgacaatgta aacaaaccaa gccactatca aggtcggttt ggaatggagt ccattgatgc
    11161 tttaagaaac ttcatgacag acgaacagtt gaaaggcttt tatcttggaa atgccttaaa
    11221 gtatttacta cgtcatcaaa agaaaaacgg tcttgaagac ctgaagaaag cacgcaagaa
    11281 ccttgattgg ttgattgagg aaatggagaa caagaatgag acctaaaaaa tatccgtaca
    11341 cagggagcaa aataaataaa gtgactacaa caggaatagg agctcaagag cttgtagttt
    11401 tccctaacgt agcctttaga aaagacttgc tcaagcacat tttctcagtt gtcaaacaac
    11461 atgacaacgc tacaatcatt tacttcagaa ttccaaaagt attcggatac gaggaggaaa
    11521 gagcaaaagt atatctaagc tacgaaaaga cgatgatgat actcaatagc tactaaaata
    11581 aaaaaagcca agacactctc tgtctcagca ataatctcaa taatattatt atatcacaaa
    11641 ggagacagag agtgaacaag gctaaagaac tattgaaaga gttgcagaat ctggacatgg
    11701 acattcaaag ccgtatagat gaaattaacg agcttgaggc aggtttgctc tcaagcccca
    11761 agtggattga cgtcaaagtc caaggtggtc aagctagaaa agttgatgat gtctatactc
    11821 agcttgtcgt gatgaaagag gccatagaac aggatactaa agaggttatc aacagaaagc
    11881 ttgagcttgg taggttgatt aacaagctga aaaatccaaa gagcaggtct attctcaggg
    11941 tgacttacat tactaagatg tatgttgatg atatttgtga caaaatggaa atcagcagaa
    12001 caactttcta cacttggcgg aatatggcta tctctgaact gaatgaggtt ttggagagaa
    12061 tggaactaaa ttgaacttta caaaaccgta cgggaaaaaa tgatacttgt tagcacagtt
    12121 ttgtaattct gataaaatgg tagtatcaag aattgaaaag agaggtctca gaattggtag
    12181 atggttacct gtaatgtcag ggggctgtaa tggccttgga ggttcaaatt ctcccctctc
    12241 ctttgagtgt ttgtgtccta gaatggggta ggcagtaggc ttagcattca tatatcactc
    12301 attaacttaa aaatggttgc ggaagcgact ggacctcgca tgattgcgta gctaattata
    12361 ttccggataa gttataagct agagggtttg attccctcag aggttttaaa gactacaaaa
    12421 aataaaaaag aagtcaaaat ttaatacgta cgcaaggttg tagtcgcctt gcaagaaggt
    12481 cgcacatcgt gtggcttttt ttgattattc gaaaggtggt gatggaaaat tgtaaagaag
    12541 aacaggaata aaagaagcag agaacaagtt gatgagtttg gtttaaatag ccgtgaacgc
    12601 aattttgcag atgagtacat cgctaatggc aataatgcaa cacaggctta tttgaaaata
    12661 tatccaacat ctggcgaagc taatgccgct aacaaaggta gtgcatgggt tagaaaaggt
    12721 gagattttaa actacatcaa aatcaaaact aaagaacgac tagatgcatc ggggttgaaa
    12781 gctaatgaca ttatagatcg tcttatcgat attgcctttg gtcgtccaat tgttggatat
    12841 agtaaacaga cagataaaat cacgggagaa gttataaagt acattgaata cgaaggtacg
    12901 gcgcctattg acgagcagat aaaggctctt gaattgttag gtaagtatct caagttattc
    12961 accgacaaag tagaagctga cgtttctgga acggtggtgt ttgcaaatga gtcagacata
    13021 ccagattaaa caaagtgata ttgtaatcga cctacctaag acagtaggag ctgggtacgg
    13081 acagttctgg cgctcaagaa gtctttatcg tgttgtaaaa ggttcccgcg gttcgaagaa
    13141 gtccaagaca accgctttga attatgttat ccgtcttttg aagtatccct gggccaactt
    13201 gcttgtcatt cgtagatatt cgaataccaa caaacaatcg acatatacag attttaaatg
    13261 ggcgtgtaat gtgttgggtg tgactcattt gtttaaattt aacgagtctt tgcctgaaat
    13321 aactgtaaaa gcgactggtc aaaaaatact gttccgtggt ttggatgatg aactcaaaat
    13381 tacatctatt acagtcgata tcggtagtct gtgttgggca tggttcgagg aagcatatca
    13441 aattgagact gaagacaagt tcagcacggt tgttgagtct atccgtggta gcttagatgt
    13501 acctgatttc tttaaacaaa tcacagtcac atttaacccg tggaatgaga ggcattggct
    13561 caagcgtgta ttcttcgatg aagagacgag acgggctgac acattcgcta ctacaaccac
    13621 ttataaatgc aatgagtggc ttgatggagt cgatatcaaa cgctatgagg atttgtatca
    13681 tacgaacccc agacgtgcta gaatcgtttg tgatggtgaa tggggagttg ctgaaggttt
    13741 aatctatgag aacgtgaccg tcaaggattt cgataaggat gaattgctac gagattcagc
    13801 taataagtta tgtatcggtc ttgactttgg ttttactcac gatccaaccg ctttgtgttg
    13861 ttcgttgata aatgatacga cgaaagagat ttatgtcttt gatgaggcgt ataaagtcgg
    13921 attgataacc aaagaagttg cgaagatgat aaaggacaaa ggttatcatc gctcacaaat
    13981 cattgctgat agcgcagagt cacggctgat tgaagagctc aggtcagaac atggcatatc
    14041 tagaataaaa gagagtcgga aaggtaagga tagtattatg gcaggcgtat ccaaattgca
    14101 aggatacgct atttatgtgc atccagattg taaaaacatc atggatgaat tttatagtta
    14161 ctgctaccag cgagataaag aaggcaactg gttgaataaa ccagaggata aaaacaacca
    14221 cttgatggac gctttgcgtt acagccttca atgtatcgaa ggtggaaaag caaccgtccg
    14281 cagacgttct gattatggtc tatagagagg aaagacatgt accaatattt aacctatcca
    14341 cgggatggat atgatgaggg ttctttgaag aaagacctga tttacaaatt gataacgata
    14401 catagcactg aaggctcgca tttgaagaag cttaaaagct actatttggg tgagcatgct
    14461 atcttagaac acacgagacg caacgtgaac gcacctaatt acaagacggt agccaatcat
    14521 gccaaggata tcgcagacac ggctacgggc tattttatgg gcaatcctat caagtataac
    14581 aatactgctg acggtgatat cgatgaacta cttacagcct ttgatggtgc tgagattgac
    14641 caagtagatg ctcagaatgc tttgaacatg gctatctatg gtcgtgctta cgagtacatc
    14701 tatgctaaag agggtatggc tgagttggat tcaactagta ttgatccgga gaatactttc
    14761 atggtctacg atgatagtat tgagcggaag cctttgtttg cggtctatta ctatgaagta
    14821 aaagacgata cgaaagacac taccaagcac caggctgagg tctttaccga aaatctgcac
    14881 tatcacatgg tgctgagaag tacagattca ggaacaactc agagcgagga ggcaacacct
    14941 cacaaccttg gtcaaatccc aattatcgag tatcgcaaca atcactttgc aattggtgac
    15001 tatgagcaac aaattagctt gatagacgct tataattcct tgatggggaa tcgtgtcaat
    15061 gataaggaac aggctgtaga gtctatactt gtcttgtatg gcacgcagtt agcagacact
    15121 ccagaagacg ctaaggtagc aatgaagatt ctttctgaag aaggtctttt ggaattgccg
    15181 ggcgatagtg caagggctga gttcttgaag aatacgctgg acgaaagtgc tactgaaatc
    15241 ttgcgtacag ctcttaaaga ggacatctac acatttagcc atgtgcctaa tttgactgat
    15301 gagaatttcg cagggaatac atcaggcgta gccatggaat ttaagctgat gggccttgag
    15361 atgattacta agaccaagga agcgaactat aagcgaggat tgcgtcagcg tattgcgatt
    15421 tttgctcatt acttaggcat gaagcagatt gctttagagt ctcattcaat cgttccacaa
    15481 ttcagtcgtg gtttgcctaa gaacttgtta gaaatctctc agattgtgaa caatttggaa
    15541 ggcaaagtga ccaatagaca gcttatttct ctcttgccgt ttgtggaaga ccctgacgct
    15601 gagctggaag ccttggaaga agagaaaaag aagaacatgg aagacatgcc gatgttcaac
    15661 aaagacaaca cgaaacccga agacgaggta gaggatgaag aatcaggagt attgggcgaa
    15721 gaggaaagcc aatctgattt accagcagat ggacaaggcc gaaaagcagg cagaccagtt
    15781 cgataaggtc tatcaggaag ccaagactta cttggataag gaagtcaata agatttttga
    15841 taagttccaa cgtgattatg gtctaagtca ggtagaagct agacaagtct tgaagaacat
    15901 gaaagacaag aaaaatctga atgaacttcg taaagtactt gaagcgagac cgaatgaccc
    15961 gaacatccaa agattactag ctgacttaga cagcccagct tattctttcc gtatgaagcg
    16021 cctagagcgt ttgagcgacg atttagaccg tatgcgtgaa tctatctatc attcagagaa
    16081 gacaggctca gacgcctttt atagcgactt gatgaaggat agttactaca aggctacctt
    16141 tgacctgcag cagcagacag gactagcata cggcttttct gggcttcctg agagcgagat
    16201 taaacatcta cagtctttca gttgggtagg tgacggaagt acctactcta cagacatctg
    16261 gaagaatacg gggaagctta cttctagcat aaaagatgaa ctacttatga gcctcatgac
    16321 aggccgagat acacgagaaa ctgcacaagc aattgctgag aggttcaatg taggtcagaa
    16381 cgatgcaaga cgtttggttc ggacagaatc agcctttttt cataaccaaa tggaactact
    16441 cagctatgaa gaagcagaca tagaaaagta tatctttgcg gccgtcttag acaagcgtac
    16501 atcacggatt tgtcaggagc atgacaatca ggtctatgat agggacaagg ctgtccctgg
    16561 cgtcaattgt ccgcctatgc acccttggtg taggtctact actgtcggat acgatgagga
    16621 cgcagattac agcaagttga agcgcagagc aaggaatcca gagacaggaa agaccgagct
    16681 agtacctgct gatatgactt ataaagagtg gtatagcaag tatgttgcga aagacgggga
    16741 aaaggtgtat aatcaagata caagagaagc caaggcgaaa ttttatagcg aacaactatt
    16801 gtccaaaatt tcaggagttg agccaaaaat tacaagtgat atgcaacgta tcgcaggaga
    16861 aaacaaattg gcaggtcttg aatttaggaa gaaaacagtt gagtcattat cacgtaaaat
    16921 tattgcagat agcctagttg aaaatataag tttgtcaaaa gccgtgagta agattaatga
    16981 cgccttaagg tacacaacta ttttcgattc cgatactttt acagaagagt atttgaagat
    17041 gaaacagaag cttatcgcag aaggttataa aattgtaaaa gtaaaaaaca cttggctagt
    17101 agatggacca tacaaaggtg tgaatacagt cgttgaaaaa gatggtatca actttgaaat
    17161 gcagtatcat actcaggaaa gtttcgactt aaaaaatggt tcattacatg aactctatga
    17221 gaagtatcgt gatacgaata catctgatct agaacgcatg aaattattta aggaaatgct
    17281 tgatttaagc aatgggcttg agattcctaa aaatatagag agggtgaagt gatatgaaag
    17341 atattaaata ctaccgcaca acgacgaaca atgctcaagt acttcgtttg attgatggtg
    17401 tcatgcaagt ttttgacatt gaaaaaaagt gggttaatag catggattgg tttaataaaa
    17461 tcttttttaa tgactttacg gattttgaag aaatttcaga aaatgatgca tttacttata
    17521 ttgacaggat ggtagcggca tgattgatat tgccttggct atcgctaaaa aagcacatgc
    17581 agggcaggta gataaagcgg gtgttgatta catacagcat cctctctatg tggccagtca
    17641 agtcaacact gaacaagaaa aagctgtcgc tcttttacat gatgtgattg aggatagcga
    17701 tataactgct gccgatttat tcgcgtctgg cttgtcaaat gaagttgtta cagcggtaca
    17761 aattttgaca aagaaaaaag gtcaaagtta tcaagaatat cttgggaaag taaaatcaaa
    17821 taatttagca agagttgtaa aacttgcaga tttgaaacat aactcagatt tatcacgttt
    17881 gaaatctgtt accaatacag actacgagcg tgttaaaaaa tataaaaatg caatttatta
    17941 cttaagcacc tagagaaatc taagtgcttt tcttatgttt agaaaggagc gagaaatgaa
    18001 atatcgtaaa aagccagtag tagttgaagc cgtgcagttt ttagatacag aagaatctat
    18061 agatgagcta tgcgattttg gattagatcc agtacggatt gattacgcag acttaaaaaa
    18121 tcctctttta aaaatcgaaa cgcttgaagg attgatggtt gcaacagaag gtgactacat
    18181 tatcaaaggc gtgcaaggag aatattatcc atgcaagccg gatatttttg cagaaacata
    18241 cgaaaaaacg gaggaataaa atgttagaaa aagcaaaaca attggcatcg caagaatttt
    18301 tacgcttatc aggtcgtgaa atcaaagcag aagactgctt tgtagtttgg tttagcaaga
    18361 ccctgcaaaa ctggaaagct cttgttagta cgaacgcaat tacatcaagc gaaccttgtg
    18421 gagattatgc agaaattacg cataacggag ataagaatga gacttatgtg gatgtttacg
    18481 ccaaggtttc aaatcgtgcc attaaagatt aggaggtgat ccaacatctt gacttgcagg
    18541 aatagactgc tataaattac tgtaaattgc tataaaccgt gtcatatttg atgcggtttt
    18601 cctatgccct aaccgtatgg aatcccgtac ggtttttata ttgtccaaac tgtgccgatg
    18661 acattaaaag ctgtactgtt ccgtcgccgg acgtaaagcg agattatcga gtggcgacgt
    18721 aatcgctgga ggacaattat gtcagaagaa atcaatgcaa ctgtatctac tgaatcaact
    18781 gagaccgtcg acactcaagg aaatgttgat acagtgcaag aagaaaagca cgaacgaact
    18841 ttcactcgtg ctgaaatcgg taagatgcta tctgccgagc gctctaaatg ggaagctgag
    18901 caagaagcca aggaaaatga agctaagaaa cttgccaaga tgaacgctga cgagaaacag
    18961 aaatatcagt tggatcagcg tgagcaagaa ctagctgacc gtgaaaaggc tattgctcgt
    19021 aaagaattga ccgcagaagc taaagcaatg ctaagtgaac gtgacttacc tgttgagtta
    19081 gtgaacgtgg ttgatttgac aagcgcagag acggtatcgc agtctgtcgc tgtattgcag
    19141 aaatcatggg agcaagccgt gcaaaaaggc gtacaagaaa agctaaaagg cggagcccca
    19201 atgaaacaag cgccagtcga tagtgacggt atcacaaaag aagaatttgc tcgtatgggt
    19261 tatcagagtc gaaatgaact ctatcaaaag aacccagaac tctataagaa attgaaaggt
    19321 taaaataaat gacagcagga caaactaaac tagccactat ggttaaccca gaggtgatgg
    19381 cggacatggt ttccgctaaa ctacctaaat tgattaaatt tactccactt gcttatgtgg
    19441 aaacagcact ccaaggacaa ccagggaata ctctaacagt tccagcatgg gagtatgcag
    19501 gagatgcgac tgaggttgga gaaggtcaag ctatttctcc agaccaattg actactaaaa
    19561 agaccactat gaccatcaaa aaggctgcta aaggttatga aattaccgat gaagctcttt
    19621 tgtcaggtct tggcgaccca ctaggtcaag ctacttatca gcttggtttg gctattgcta
    19681 acaagattga tgatgatttg gtcgcagtag ctaaaactgc aacacaacat attacagaaa
    19741 ctcctacaac tcttgcagca attgataaag ctcttgagat ttttgaggac gaagaagatg
    19801 cgcaatatgt tgctatcatc aaccctaaag atgctatcaa gctaaaaact gacgtagcaa
    19861 aagaatggac taaaggttca gagcttggtg cagatatggt tgtatccgga acgttcggtg
    19921 aagttgccgg tgtgcaaatc gtccgttcta aaaaagttga tgaaggtaaa ggctttatcg
    19981 tcaaagtctc tcctagccaa actcagacag acgatgccaa caaatatggt gcgtttgtta
    20041 tcatgctaaa acgtgatgtg gctatcgaaa cagaccgtga catccttaaa aagacaacgg
    20101 ttatcactgg cgatgaacac tatggtgttt acctatacga ccctacacga gttgtaaaat
    20161 tcggtgagta agaggtgacg atatgagctt attgctacga cgtcattata tccaagagga
    20221 gcaggttagc cagtattctg atttagagaa taagactcta gaagagttga agaatctagc
    20281 caaagaagct ggcatagctg gcgcctataa gttatcaaaa gccgaaattg tagaggtgct
    20341 ggaggattta aaaagtgaaa tttaaaatca aacaagattt ctatgattgg gaatcaaatg
    20401 tgaaacgact ggcaggaggg gaacttgaga ttactgagga gcgctatgtt gagctggctg
    20461 acaatattgc cagcaacggt gtcgctatct cagatgttct tgagaaaatc ctccctgaac
    20521 ctgagttctt agaagaggat tgatatgtct atagagttgc tgaagaaatt aacaggcgaa
    20581 gaagatactc agcttctcat gttgctccaa acaagggcta caaatcttat cttgtcagag
    20641 actaatcgca catctttgac acctgcttta agtcttttaa tacctgaggt tgctatcgag
    20701 ctccacaacc gctcaggagc ggaaggagag cgttctagaa ccgaaggtgg tatagaagta
    20761 gtctacggag aaaacggcct gtctacgggc cttttacagc gtatacgtat gcatagacta
    20821 gcaagggtgg caggccatgt ttttgaagca gagtagactg aaaccttatc caatgcgacg
    20881 gtttgaaaag actgtcactg aggaaggtgt cgcaaaagaa ggatatgtca aggaagctga
    20941 gacaatccgt cttgagttgt ggccagctag tagtaaacta cagtctgaat tgtatggcga
    21001 gcgtgtcaat gatattttga acgcaaatgc caacaagtca gctactatca aagtgaaaga
    21061 tggtgtgtgt atcgatagcc agacagaagt gactcacagg gttatttcta aaaaggtcta
    21121 cacacatcat caagttttgg agttagagcg tgtcagggct actaggggca gataggctta
    21181 tagctaaatg tagacgattg gctagtaaaa aaactggcga ggatatcgtc ttacgtgcgg
    21241 tacacaatgc tactataaag gttgtccaag cagatgcaag aagactcgca ccagcgagag
    21301 atggagagct tataactagt atcaaaacta gggcaaaaat ggacggagat aaggctatag
    21361 gcgaggttta caccaatcta aaatatgctc cttacgttga gtttgggaca gggccaatag
    21421 gacaagctag ccattcgggt atctctccag aggtcagcgt cacttataag tctagtccgt
    21481 ggtatgtgca tgaagaccaa atcaatgtag gaccttacca ttttcaaaaa attggggagt
    21541 tctacaagat gtatggtcaa cctgcccagc cttatcttta tccagctttg agagacaatc
    21601 aagagcgtgt gtctaagaat atttcgaatt atgtccgtag aaagataaga gaacaaataa
    21661 aatgattaat atcaagcctg ttatttataa agaattgcaa aaggttgcag ataatgtgac
    21721 cgatacttat cctagcgatt gggagaattt cccagtcgtt atttttttag aagaacagaa
    21781 caagccgggt gattggtttg atgaccagga acaaaaatca tctatccgct ataaggtgga
    21841 tatctttgat gataccagca ctagtgagtt agctgttaaa atcaatcaga tttttgagtc
    21901 tttaggtttg cgaagaaccg actgccaaga cgtgccagac ccgtctcatt tgagacataa
    21961 ggtcatgcgt tttgaaggtg tcgttgactt agactcagag cttgtttttc aatttagaat
    22021 ggagaattaa acatgttagc aaatggaatt acgctttctt atagcaaaac aaaaggtagc
    22081 tatactaagc ttgttgggtt gaaagaagta ccagagtttg gtattgagcc tgaaaaagta
    22141 gagaacacta ctcttgaaga taaagttaag aagtatgagt tcggtattgg agacgcaggg
    22201 gaattggaat ataaattctc ttacaaaaac gatagcgcaa ctgctcctta ccgtgtattg
    22261 cgtaaggcag cagacgacaa ggaaaaactc tacttcgaac aagcttatcc agacggtact
    22321 aaggtcacat ttgaaggtca agtgtccgtt aaattgggcg gtggcggtgt caatgccgtt
    22381 atcgatttca cacttaagat tgccttgcag tcagagttgg aatttacaga cggtgttgga
    22441 ggttaattaa atggcgttaa aatacacaac ttggaaagtt actgacgaaa aagagttgaa
    22501 gctacgtttg acatctcatc aagctgcaac tgtggaagaa aaaatcggca tgaacttgtt
    22561 aaagattttc atgcctgaag ctggcgaaga gttcacttta ccgcctttga aagttatgct
    22621 gttgttagtt catggagcct tgcagcagta tgaacatggg tattcctttg aagatgtcta
    22681 cgacctatac gatgaatacg tggacaatgg gggagaccaa acaactttca tgacagaggt
    22741 tttaatgcca ctatttgaag tatcgggttt tactccacga ggaagcaaga acaagaaaac
    22801 ttccaagaag aaaatgacag tagtcgagta atcttaacag taacgcagat tattgagagg
    22861 ctttatccta tgtttttgga cattggaggc aagcctcttg atttttggga tttgacggtg
    22921 cttgaaatca gagaaatgat tgaaagctac aaccgtgtca aaacccaaga gcgtaaagag
    22981 aagattattg actcatacag actttcgcag atgatttcca atcacgtttc tttattactg
    23041 tccaatgacg ctaagattgt tgagttctgg gagtatgcgc ctgagttatt tgtagaagaa
    23101 caacaagcgg tagaactgga acgacagaaa caagcacttt tgttgcataa ggaacggatg
    23161 cgtgaatttg cagagagaca taatcgaaaa aggaaggagg aagtaaatgg caactcttga
    23221 tgaattgaaa gtcatgattg acgctgagat agcgcctttc aggaagaaga tgaaagaagt
    23281 cgagaatcag gtcaaaggaa catctgacca agtgaagaat gccactgcca aagttcgtga
    23341 acagtcgaac tctatcggta gtgcgtttgg taagctggct aagttcgctg gttttgcaat
    23401 ccttggtaag aaattacttg atgttgggat gtattcaacg cagacggctc ttgaagtatc
    23461 agcgtctatg aaccaaatca agcgacagat gggcgagagt tcgcaatctt tcttaaaatg
    23521 ggttaacgat aacgccaacg ctatgaatat gggtgtgggt gaggctacta actacggtgc
    23581 agtctactca aacctatttt ctggatttat taaggacacc aacaaattga gcgcctatac
    23641 tgctaaaatg ttgcagacgt cggcagttgt cgctgaaggc tcagggcgca cgattacaga
    23701 cgttatggag cggattcgct caggtttgct agggaacacc gaagcgattg aggatctagg
    23761 aatcaacgtc aatgtggcta tgattgagtc cactgaagcc tttaagaagt tcgcaaacgg
    23821 gcaaagttgg caacagttgg attaccaaac ccagcaacaa atccgcctta tggctattct
    23881 ggaacaggct acagccaagt atgggaatac cttgtctaat tctgtaaatg gtcgtatcag
    23941 cctgtttaag tcgctgatga aggacgcagc attgaacctt ggtaactcta tgttaccgat
    24001 tatcaatgcc attatgcctg tcttgaactc ttttgctatg gttttgaaga acgttactgc
    24061 taaactcgct gagtttatcg ctttgatgtt caacaagaaa gcaacagtga aagatggtgt
    24121 tggtggagca gttggagaca tgggtaacgc catgaaggat gttgcaggcg gagcaggaga
    24181 ccttgctgac gcagtagacg acgctggaga ttcagcagga ggacttgctg acaatcttgg
    24241 agactccgcc aaaaacgcta agaaggccgc taaagagttg ctaggtcttt tgggatttga
    24301 tgagattaac atcttgcaaa aaccaaaaga tgacgacgca ggcggttctg gaggcggtgg
    24361 caaaggtggt aaaggaaagg gaggcggtgg cggacctttc aaagacatct tgccagaagt
    24421 cgagttgacc gacatggaca acaaattcaa gagcattttt gatggtcttg gagataagct
    24481 caaagggttg tttgacctct tcaagaaagg ttttgatgca gcatttagac cagaaggtat
    24541 aaaacgcatt aagactgcct tagaccaaat agctaagaca atgggagaaa tcgccactga
    24601 cccaagggtt gtgaatgcct ttaaccgaat ggctgagaaa attgcttatg ctttagggca
    24661 agtgacaggc tcaataacca ctatcgggct aggtatcggt gttttccttg ccgaaagtat
    24721 tgcaaatggc cttggaaggc aaaaagaacg cattaccagg gcgctagtcg ctttgtttga
    24781 taatgttggt aacctttccg aggcagtagg aaacatagct caggactttt ctagtgcttt
    24841 ctacgacgtc attacctcaa ctggtgcggt tcgtatcggt agcgctattg tgtcaactct
    24901 gttgagtttg acatctacca ttgttgaagt tggtagtaaa ttagcaggaa gtttgtttaa
    24961 aggttttgaa aaattcgttg tgacaagcgc tcctaaaatt tcatcagtct tccaaagttt
    25021 attagatact gttgcgcctg tatttgagag cattgaaagg tctgttaaca aatttggcga
    25081 tggcttaagt cgtgtttatg atgaacatgt agtccctact attaactcta ttgctaatgc
    25141 ttttaatggg ctaattgaca ttattcagat tctctgggag aattcctggc aaccttttgc
    25201 tgagttttta tcaggagtat tcggtgttag tattgaagga atttcagatt tattaggagg
    25261 tggcctttta gccactttgg gactattggc ggatgctatt aagttagtgg cagatggttt
    25321 caccgttttt tctgactggt gtaaagaaaa caaagaacct atcgtagctt tgataacaac
    25381 ttggcaaacg attaatttct tatcatgggc agaacaagct ggaggacttg caggagcatt
    25441 cagcttgtta ggtagtaagg tctcttcgat tgttggagga attaagaatc taggtcttgc
    25501 tattaaagca ttgacatttg ataagttggt cagttttggt gaaacaatct atttgaacac
    25561 cttatatgca aaagattttg tggtcaattc aggtaaaaca attgcacagc taggaaaaac
    25621 tgctttagaa cttggtaaat cagctctagc atggactgct catgcagcga aaatgggatt
    25681 agcaaccgcg gcgaaatttg cacattctgt tgcaacagga gtcgctacag ctgcaacatg
    25741 ggcttttaat gcagcgttag cagttttgac aagtccaata acatggatta ttgcagcaat
    25801 cgcagcctta attgctatcg gtgttttgct ctatcaaaac tgggacactg ttgttgagtt
    25861 tgctaaaact gcatggcaag gactatgtga ttttatcagt ggtatttgtc aagcgattgg
    25921 cgaatttttc agcggtctat ggacgaaact acaagaaatc tttgagccga taggtcaatg
    25981 gtttggcgag aaattccagc aagcatggga cgccattgta aacatattct ctggcatcgg
    26041 agagtggttc tctggtgtat tccaaggtgc atgggacgct atcgttaata tcttcacacc
    26101 aatcggctca tggttcggac aacgttgggc agatgtgact agtgcgttgg ctaatatcgg
    26161 ggcatggttt actgacatgt tccaaaaagc atggactggc ttaacaaaca tctttagcaa
    26221 actaggttca tggtttggcg agagatgggc agatgtgact aatgcgttat ccagtgtttc
    26281 aaactggttt ggtgagatgt tcactaatgc ttacaacgca gtaaaagatg cttttagttc
    26341 tattggcgac ttctttaaag gcgtttggga tactgttaaa agtatcttcg taaatgctgg
    26401 tcagatggtc ggagaggcag taggtggagc gtttaagagt gcggtcaatg cggttcttgg
    26461 aacgattgaa aatgtagtca atggcttcat cggaatgatt aatggagttt taggcgttgt
    26521 cagaaactta cctggtctag gatgggttgg tagtgtaagt acagttagcc tccctcgtct
    26581 tgcccgtggt ggtatcgtcg atagtccaac aatcgccatg attggtgaag ctggtaaaga
    26641 ggcggtcgta ccacttgaaa atacaggatt tatccaaaca cttggacgag tagtcagcag
    26701 tgcggtagta aatgccatgg ctggtgttag tccacaaggt ggattctctg gcgacggcga
    26761 catcgttatc caaatcgcag gccatgagtt cggacgggta gccatccaag aaataaacaa
    26821 ggaacatgaa cgagcaggtc aaaccttgct caagatttag gaggttaaat ggcacaattg
    26881 acaatcaatg gagtggctgt gaagcctccc aaatattttc aagtcggtat tcaagatatc
    26941 gatggagaga cagggcgtaa tgccaatggc gacatgatgc gtgaccgtat cacgaccaaa
    27001 cgcaaactag actgtgaatg gggtatgatg actcagggag aattaagtca gcttttacat
    27061 gctgtatcat ctgaattttt tgaggtatct tatccagacc ccatggatgg ccaagtcaca
    27121 aagactttct atgtcggtga taggacagct cctagctata cctttactga gaagtttaaa
    27181 ccttggtctg gcgctaaatt taatctggta gagaggtaag aaaatggacg ctttaactag
    27241 acgacaattt gacagagcca tgtttgccaa ggaaaggacg ctggctattc gtgttggtga
    27301 ttatgcttca cgggatatca aagaggctag ttttgagtat ggctacatta agggcgatac
    27361 ttataagcct ggtggaacct gcgctggtag cggtaaaatt acctttacca gtatcattac
    27421 cacgttcaat aagctggata ccctgcaccc tgagattggt ctactggttg gggataccta
    27481 ccagtgggtc aagatggggg aatacttcat caacgatatt gagattgacc gaaaccgaaa
    27541 cactaccaca cttgaactta tggatggtat gtttaagctc aatcgtgagt atgtgacaga
    27601 tttgcatttc ccagctgaag tacgagaggt tattcaggaa atctgcctga aaacaggcat
    27661 tgagttagcg aatgactatt tcggaatcag cgcgatgcgt tatcatattg agcaagttcc
    27721 tgagggcaag aaactttcct ttagggatat gctgagcgct atgactcaga tgattgggat
    27781 gtcttgtttc ttcaacagag aaggcaagat ggaaatccgt gatttgactg agtccaatat
    27841 cacgatcaac gctgacagtt acttcttgca tggcttgacc aagagtgaga ttgagtatca
    27901 gatagctggt atcacttgta agacggacaa gaagtctctg acggtcggta tgaagacagg
    27961 ccggtctttg gaactggaca atgtcttcat gacccagagt gctttaaatg acctgtatta
    28021 caaactgaaa aacctaactt actatccgta taatctcaac taccaagggc atttactgct
    28081 tgaggttggg cagtgggtaa ccattcagac caataagaaa gagactttta aagttcctgt
    28141 cttaagtcag agctttactt tcaaaggtgg tctgagagga cgtatcagcg cagatagtaa
    28201 agctggaaat gatactcagt attcttacga gggaacgatt accaagcaga taaagcaaca
    28261 agatggcgtt gaagcaaaaa tccaagcgca gattgaagca gcagataaag attttgacca
    28321 aaaggtcgac aaaatcaaaa aagactttaa cgatcaagta gaacttgcca aagccagagc
    28381 tgaagaagtc aagagagaac tgtctgacac tatcaatcag cgctttaata gctttgacaa
    28441 cgggccattg aaagaaacta agcgcaaggc tgaggaagct ttgcgaaa
//
LOCUS       DAVMHG010000010        26548 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMHG010000010 DAVMHG010000000
VERSION     DAVMHG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.010"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..493)
                     /locus_tag="VYR08_000359"
     CDS             complement(<1..493)
                     /locus_tag="VYR08_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV3465944.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKI"
     gene            complement(720..1589)
                     /gene="rsmI"
                     /locus_tag="VYR08_000360"
     CDS             complement(720..1589)
                     /gene="rsmI"
                     /locus_tag="VYR08_000360"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV3465945.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(1592..1909)
                     /gene="yabA"
                     /locus_tag="VYR08_000361"
     CDS             complement(1592..1909)
                     /gene="yabA"
                     /locus_tag="VYR08_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV3465946.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1949..2839)
                     /locus_tag="VYR08_000362"
     CDS             complement(1949..2839)
                     /locus_tag="VYR08_000362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV3465947.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2836..3474)
                     /locus_tag="VYR08_000363"
     CDS             complement(2836..3474)
                     /locus_tag="VYR08_000363"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV3465948.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(3694..4491)
                     /gene="proC"
                     /locus_tag="VYR08_000364"
     CDS             complement(3694..4491)
                     /gene="proC"
                     /locus_tag="VYR08_000364"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV3465949.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLILEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(4495..5757)
                     /locus_tag="VYR08_000365"
     CDS             complement(4495..5757)
                     /locus_tag="VYR08_000365"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV3465950.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(5767..6897)
                     /locus_tag="VYR08_000366"
     CDS             complement(5767..6897)
                     /locus_tag="VYR08_000366"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV3465951.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6977..8860)
                     /gene="cbpE"
                     /locus_tag="VYR08_000367"
                     /gene_synonym="pce"
     CDS             complement(6977..8860)
                     /gene="cbpE"
                     /locus_tag="VYR08_000367"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV3465952.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNFERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIVGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(8863..9750)
                     /locus_tag="VYR08_000368"
     CDS             complement(8863..9750)
                     /locus_tag="VYR08_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3465953.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9740..10201)
                     /locus_tag="VYR08_000369"
     CDS             complement(9740..10201)
                     /locus_tag="VYR08_000369"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV3465954.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10198..11106)
                     /locus_tag="VYR08_000370"
     CDS             complement(10198..11106)
                     /locus_tag="VYR08_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3465955.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11099..11296
                     /locus_tag="VYR08_000371"
     CDS             11099..11296
                     /locus_tag="VYR08_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3465956.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFLRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(11348..12019)
                     /locus_tag="VYR08_000372"
     CDS             complement(11348..12019)
                     /locus_tag="VYR08_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3465957.1"
                     /translation="MKKRAIQILLVLSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12073..12294)
                     /locus_tag="VYR08_000373"
     CDS             complement(12073..12294)
                     /locus_tag="VYR08_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465958.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            12566..13375
                     /locus_tag="VYR08_000374"
     CDS             12566..13375
                     /locus_tag="VYR08_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3465959.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(13556..14431)
                     /gene="aguB"
                     /locus_tag="VYR08_000375"
     CDS             complement(13556..14431)
                     /gene="aguB"
                     /locus_tag="VYR08_000375"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV3465960.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(14441..15526)
                     /gene="aguA"
                     /locus_tag="VYR08_000376"
     CDS             complement(14441..15526)
                     /gene="aguA"
                     /locus_tag="VYR08_000376"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV3465961.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(15523..16650)
                     /gene="nspC"
                     /locus_tag="VYR08_000377"
     CDS             complement(15523..16650)
                     /gene="nspC"
                     /locus_tag="VYR08_000377"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV3465962.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(16650..17909)
                     /locus_tag="VYR08_000378"
     CDS             complement(16650..17909)
                     /locus_tag="VYR08_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV3465963.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(17906..18766)
                     /gene="speE"
                     /locus_tag="VYR08_000379"
     CDS             complement(17906..18766)
                     /gene="speE"
                     /locus_tag="VYR08_000379"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV3465964.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            19021..19624
                     /locus_tag="VYR08_000380"
                     /pseudo
     CDS             19021..19624
                     /locus_tag="VYR08_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(19701..21176)
                     /locus_tag="VYR08_000381"
     CDS             complement(19701..21176)
                     /locus_tag="VYR08_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV3465965.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            21883..22878
                     /locus_tag="VYR08_000382"
     CDS             21883..22878
                     /locus_tag="VYR08_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV3465966.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            22904..23548
                     /locus_tag="VYR08_000383"
     CDS             22904..23548
                     /locus_tag="VYR08_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV3465967.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(23590..25578)
                     /locus_tag="VYR08_000384"
     CDS             complement(23590..25578)
                     /locus_tag="VYR08_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3465968.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(25580..26338)
                     /locus_tag="VYR08_000385"
     CDS             complement(25580..26338)
                     /locus_tag="VYR08_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3465969.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
BASE COUNT         7805 a         5980 c         4893 g         7870 t
ORIGIN      
        1 tgattttagt tctgaaagtt ccaaaaggtg caaccgtatt tacatgaata aacttataca
       61 cttcccaagt cgctgtttta gtcgcttcat cagcccactt tctcatatgc ggttcaaata
      121 attcttcctc actcaacgaa tcaatcattg cagagataga atgaatattt tcatttaatt
      181 ttgctttcaa ctcttgcaga gataaatgag cgtaggtatc tgtgaaccac tgatataatt
      241 caccaagttg attccattta aatttatccg atggtgtttt tacttgaagc ccctttcttt
      301 catcttcttc ccatttaaga accaagttgg tccaaccaac ctgataagca aggttttctg
      361 ctggagttct gtcaacttca tcagctctct tatcttttaa attttctgga atattatcaa
      421 attctaaaat atatttctca aatgttttct ctatctcagc ttttagttct tctttatttt
      481 cgtatgttcg caaaaaatca ccttctttct gatttatcaa tcctttgatt taaaatgacc
      541 ttatttttct atttatacct tctaagaacg ctataactgc tgctccaacc atcggtattc
      601 cgtatgggct taataaaaaa cttaatacca aagcttctat accaagtgat acatctttat
      661 attatcatca ctaattaagt taaacagaaa cagacagaat tattacatcc tgtctccctt
      721 tattgttttt cttcccagtc gtggtaggca gcgtagagct gacttttatt ccactggtaa
      781 atcttagcga cttccttgat agcttggttt ttcttcacac cttgctggat gcgggtttga
      841 atttctacga acaagtcttc ctcgtccttt tcctccacac cctgactggc accctcaaca
      901 atgagaagac attcgccctt gagtggcgtt tcagcaatgc tttctaataa ctcagagata
      961 gtacctcgtt ggtattcttc atagattttg gtcaattccc tgaccaagac aacggagcgg
     1021 tcaccgtaga cttctaacat attttccaac gtgtctgcta cacgatgagg tgattcataa
     1081 aaaatctgtg tttcaggata atcttttttc aagccaaaaa attgcttctg ctgacctgat
     1141 tttctcggta aaaaaccgta aaagatatgt ggctgtggcg ctaaaccact ggcaatcaag
     1201 gcagaaattc ctgcagaggc acctggaact gtcacaactg caatttcttc ctcaatagct
     1261 gccttaacta aatcatgacc agggtctgaa atgctaggca aaccggcatc agagacctga
     1321 gcaatacttt gccctgcttt caagaaacca atcaaatcag gaattttttc cttggcattg
     1381 tgctcatgaa aactgatctg cttggtggaa atgtcaaaat gcttgagcaa aagccctgta
     1441 ttgcgcgtat cctcagcagc aatccagtcc acttctttca aggtctggat agcacgaaaa
     1501 gtcatatcat ctagattgcc aatcggcgtt gccactagat acagcttgcc atagggagac
     1561 tgccccttaa aacttttttg aatctgcatg cctactccct gtatagcaac tcgtcacaaa
     1621 acatacattc ctcgtcctgc tctcgacgtt gtccataaaa atcattacat acgtgaaatc
     1681 catcacggta aatgcgacgg acactttcac gaacatgctt ggccttgaca ggagcatctg
     1741 cttccacctc acccaagcgt tctcgcaact tagaattttc caaacgaaga gctgtatttt
     1801 cctctaccag gctcttgaga tttttcttga tggcttccac atcggccaag gttaccaata
     1861 actgttggga aaaatcatcc agcgcgtcaa ataattcttt tttgtccata aaacagccct
     1921 ttcctttctt tatcattcat ttttgagttt atatttcttt caagaccaga tattccatgg
     1981 cattttgaaa gctgacatta gcttgccaca tttttctagc ttctagtaaa tcttgtagaa
     2041 tcactcttac tcttacctgc aagaggtcct gcccacagag aacttcaaga atccgtaaaa
     2101 cctgatcctg tttttcctta tcatctgcca agttggctaa tttggcaacc tgtagataac
     2161 tttctttttt cttagctact aaccaagtca gcaggcgttc actttcatcg accaaggtcc
     2221 aaaaacttgc ctgatgtgcc aacttttctg cttcagctcg cgattgacta aacttagcta
     2281 aaagagtcgc ttttttctta acaagtccca tttgttctaa gagtaagata agtttttctt
     2341 cttgcttttt aaagtggaag atctgagtcc gacttcggat tgtcggtaac atcttttcct
     2401 catcgctagt caagaagaaa atataaactt cactctgggg ttcttcgatg accttgagca
     2461 gagaattggc tgcgttggga tgcattttat ccgcttgctc gataataaag acctgttgct
     2521 ggctttcaat ccctgcttga gaaaactgtc ccaccaattc ccgaatgcgt tctgtcttga
     2581 tgacctgatt gactggctta atcaaggtga catctggaaa ctcttcctgt tcaatcagct
     2641 tgcaacttcg gcatttctca catggtaaga cgccaacttt atccgtacaa aagaggctct
     2701 tagctaaaaa ttgcgccatt tccaagcttc caaagaaacc tgaaaagaga taggcgtgat
     2761 tgagctggtc ttgttctaag atacggacaa aacggtcaaa ctgagctggt tgccaagcct
     2821 ttagttgatc ttgtttcatt tggccaagtc cattccgtca aacaagacag ccttggtagt
     2881 ttccacaact tgctccaaag ggagactagc atcaatcttg acaatgcgat ttccttcttt
     2941 ttccaggaga gaaaggtagc cttgacggac tttcttgtgc aattccaaac cttccaagtc
     3001 cagacgattg acctcgcggt cactattcgc agcaatgcga gccagccctt cttccacctc
     3061 gatgtcaaaa tagagtgtca aatcgggttt gaggccatct gtcgcaaact gattgagcca
     3121 gtcaatggct tcaatatcta agccacgacc aaatccctga taggcaacag aactatcgat
     3181 aaaacgatcc atgatgacca acttgccagc ttcaagggct ggaagaactt tttccaccaa
     3241 atgctgtctg cgactggcaa tatagagaag tagctctgtt ttagcatcca tctgagtatg
     3301 acttggatcc aaaatcactt cccgaatctt ctccccaatc aagactccgc caggttcacg
     3361 ggtcgtcagc acctttactc ctttttcctc taaaattggt agcagagcct ctaaaacact
     3421 ggtcttgcct gctccctctg gtccctcaag agagactaaa aatccttttg acatgtctaa
     3481 ctcatttctt ttttactact tctattctat caaaaaaata ggggtttgtg acaatctttt
     3541 tgtcttctca tttcatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc
     3601 tgtacttgag tacggcaagg tgaagctgac gcagtttgaa tttgattttc gaagagtatc
     3661 aaccaccata aaagaggcag ataaaactac ctcttattta cctagttctt gtgttcgttt
     3721 ataggcttga tgaactgcct ccatgaccgt tcctcgaaaa gcatgcgctt ctaggcttgc
     3781 tacgccagca atagtcgaac cgcctggact acaaacttga tctttcaaaa ctccaggatg
     3841 ttgctggctt tctaggacca attgcccagc tcctaccaca gtttgggctg ccattttcaa
     3901 ggctgtttct cgtggtaatc ccgtctgaac acctgcatct gccaaggcct caataaagag
     3961 atagacaaag gctggtccac agcctgcaag acctgtcgct gcattgatta aactttctcc
     4021 taattcaacc aagagaccag ccttggataa aagctgacaa aagagctcac tgtcctcagc
     4081 cctgcaatta ggagacaagg cataactaat cactccttgc ccgatagaag caggggtatt
     4141 aggcatcata cgaataattc ggtgttggct tgggataaga cttgctagtt tttctaagat
     4201 caatccagct gccatcgaaa tcaaaagaag actttctctt ttttcaagga tggtctggta
     4261 ttgagaaagc agttcagaaa actgagcagg cttaacacct agaaaaatca catctgcttc
     4321 ttcgaatatt tcttcattgt tagaagtctg accaccgaag ttagcgatga aagcatccac
     4381 cttgacttga ctacgattgg caaggagaat ctcatctgac ggtctagcct gcaagacaga
     4441 cttggctaaa ctagcaccca tattccccaa accgataaat ccaatcttca tctcttactc
     4501 ccttatctgt ccatcaccag tgaccacata cttgtagcta gtcaattcct tcaagcccat
     4561 aggaccacgc gcgtgcaatt tctgagtaga aatccccatt tcacaaccaa gaccaaattg
     4621 tcctccatct gtgaaacgag ttgaggcatt aacatacacc gctgcagagt ccacttgatc
     4681 tgtaaagtat gctgcagctt cagcattttc cgtcacaatg gcatccgaat gatgggtact
     4741 gtgggattca atatgcgcaa ccgcttcttc taaactgctc acaaccttaa cagctagaat
     4801 atagtctaaa aactcggtat caaagtcttg tgcttgagca gcttgacctg aaacaaactg
     4861 gcttgctttg ctatctaggc ggaattgaat tggttccaac ccagcttctt ttcgatctgc
     4921 aaccaacact tgctccaagc gaggaaggaa gcttactgcc ttgttttcat gaaccagcag
     4981 aacctccatg gcattacaaa cagaaggacg actggtttta gcattgttga tgatagacag
     5041 cgccttgtct tcgtctgcat ccttatccac atagacatgg acaatcccag tccctgtctc
     5101 gataacaggt acaatcgcat tctcaaccac tgcattgatc aagccagctc ctccacgagg
     5161 aatgagaagg tctagatagc ccttggcctt catcatagca taactacttt cacggctagt
     5221 atcctccacc agttgaatca catttggatg aatagtagtc gtctccaagc ccttcttcaa
     5281 ggctgtgaca atggcatggg ttgtttgata ggcatcctta ccactacgaa gaacaaccgc
     5341 atttccactc ttaagagtca aagcagccgc atcagacgtc acatttggac ggctttcata
     5401 gataataccg atgacaccca tagctacacg tttttttgtg ataaccaaac cattttcaag
     5461 ctgacttgtt tctaaaactt caccgattgg atctggtaag gcaaccactt cacgaattcc
     5521 tcttgccatc gcttctatcc gatctgcatc caaataaaga cgatccaaca tcacatctga
     5581 gattttcccc ttagccgctg ccatatcgag ggcattagcc gctaaaattt cctcagtagc
     5641 agccactaag tgatcagcca tggctagcaa ggcttggttt ttcacttctt cactagctgt
     5701 gttaatcgat tttttaacag cctgtacctg ttcaaattgt tcttgtctac tcaccatagt
     5761 ttacctctaa aattctgtaa aaagtagttg gatttcagga gtaatggaaa tccagtcgtc
     5821 acggtaaatc aagacaccct tggctttttg agaacgcaac atatcctcca aagcagatgc
     5881 tccaaattgc acgcgtcctt ttccaagtga ttttccactt tccttgtcaa atactgtcac
     5941 gatatcaccg taagaaaagg ctccttctgc ttcaacgata ccagataaga gaagactctt
     6001 tccatgttga gagagagctt ccgcagcccc tttatcaacc caaatagaac cttgactctg
     6061 agcatagaag gcaagccatt gtttctgggt acgaagcccc ttctcttgag caacaaagta
     6121 agaaccatcc tcagtctcct ctgccgcctc aatcatggaa tctgatttca aggatgagca
     6181 gatataaaca ggaactcctg attccgtcgc gatagttgca gccttgattt tggttaacat
     6241 acccccagtt ccgtttgacg aacctgctcc accagccata tcaataatct cacgattgat
     6301 ggtctcgatt ctctccaagc gtttggctct tggatctgaa ttaggatttc cagtatagag
     6361 accgtccaca tctgtcaaga aaactaaaag gtctgcttgg accatcgccg ctacttgagc
     6421 acttagagtg tcattgtccc caaccttgag ctcatcaata acgacactat cattctcatt
     6481 tatgatagga attgccccac ggttgagcaa aaccgacaaa gcctgatggg catttttata
     6541 acgacgctta tccacaaagt catcttgggt cagcaagatt tgtgcagaaa cgatttgacg
     6601 caagagaaga ttggtcgtat attcttccaa caaaagtccc tgccctaccg ctgctgaagc
     6661 ctgtttatca gcaattttag tcggacgctt tttaaatcct aaggctccaa aaccagccgc
     6721 aatggcacct gaagacacca aaatcaactc atgaccagcc tcgtgcagca tagccaactg
     6781 ctgggtaata tcctttacct tactacgtga taaacttcca tcctcatttg tcagagaaga
     6841 agtacccacc ttaaagacaa tccgtttgta tttcatagtc tcactccgat tattcatatt
     6901 tatccattat aacatgaata acaggaaata gaaaagagtt gataggaaaa agaaggccct
     6961 aaaaccttct tttctactac tgttctgatt ccgatttgtt tacactcgtt tcagagcttt
     7021 gtgaagttga ggatgtcaaa tcttgactag ttgaagaatt tgtaacactt tcatgttgac
     7081 tcgtttcttt attttcaaga tcctttttca ctacttcttt ggattctttt atttctgaat
     7141 gacttgtagt tttaattgta gcagatttct cgatcggaac atccactaac caggcaccac
     7201 ttgaatccac agtatagcct tctggtgtct taccattcac aagtaattga ccattttctt
     7261 tcaagaagta ccatttatcc ttgtctttaa tccaacctat agctcgtgaa ccatctgtct
     7321 tgtagaaata ccaatcattt gcctttttaa gccagccttg cttcatagct cctgattctt
     7381 ctagatagta atgatgtcca gcaacttcta tctcacctgt cttcatgaca ccagtagaat
     7441 ccatatagta ccaggtttct ttatctttta cccagcccat tttcatttcc cctgatggag
     7501 caaagtaata ccattgcccc atatattgaa gccatcctgt cttcatagaa ccatcaacat
     7561 ccaaataata ccactgctct ttatcttgaa tccaaccaat ttccatctgg ttttctttgt
     7621 taaaataata ccagattcca acaattttct tccaattttt agcagcaaca ccagagtctg
     7681 tcaaatagaa ccaacgattg ttccattttt tccattgatt ctgtaacaag atacccgttt
     7741 ggttaaagta ataccattca cccttaattt cattccaacc gacagcatat tctcctgtag
     7801 aatcaggcgc ttgataccac cagtttccat atgaactctt atgccaacca gcttgaaaac
     7861 ttggaatagg cttgtaggat gttgaaatat tgacaaaacc gtcttttcga atatcaaaaa
     7921 ctgtcgcatc atagtctttg ctggctgcgt tgattctctc aattcctcgt tctttgagcc
     7981 aattaacata ctcactatca acaccatttt tccaaggtag actatccgaa gtttgaacaa
     8041 tcaaactcgg actcaaattt ttaatgaaat ccttggtatt tgatttgttg gtatcatggt
     8101 gatgattaaa cttcatcaaa tcaacttttc caatgagagg accatacttg tcttctgctc
     8161 catgaacatt atctaagtcg cccccaaggt aaattttctt gccattgact tttaccacac
     8221 taatcaagga attggaatta tcatcccaaa ttttctttaa ttcacctgat gaatcggttt
     8281 cattttcata gttatagagt tgaatatcca tgtccccaaa ctgaaaatga gcatctccct
     8341 gtgtgatgtt ttgaataact gaaacacctt tttctgaagc agtctgtaaa accttatcat
     8401 aaccgtacag attatcccat agacgttcaa aattagtaat acgattatca ctatattttt
     8461 taagatagac tcggtcaact ggataggtag acagtaattc atcaacattt ccaatatgat
     8521 cactgtgggt atgggtcacc aaaataaaat caagtttttg gacacccaat tccttcaaac
     8581 gacgaaagac acggtctgtt agaacatgct tataagacgt ttcaattcct tctctccatg
     8641 gatagcgaga atcacttcca tctgggaaat cataatcttc tcctgtatcc accatggcaa
     8701 aatgtccatt gctttcaaga ataatcgcat cactgccacc ttcttgaaca ttgataaagt
     8761 ggattttatt tcctgaactt tcttgagcct gaacattccc ataccatgac aaacctaaaa
     8821 aagcgcctac aagtgctaaa ctagttaatt tctttttcat tcttactttc tcaatctctc
     8881 caaggtttcc ttaaaaatct ctgggatatc tgctttaaat tccaaggtct tacctgttct
     8941 cggatgagta aaacctaaag tcttggcatg aagaaattgt ccatgtcctt tcaaagtctt
     9001 gcgaggacca tagacctcat caccagcgac tggatggccg atataagcca tgtggacacg
     9061 gatttgatga gtacgccctg tctccagttg caactctact aagctataat cgccaaagcg
     9121 ttccaagacg tgaaaacgcg tcactgcagg cttcccttta gcagttacag cctgtttctt
     9181 acggtctttt tcactccggc caatcggcgc ttcaattaca ccacgatcat taggcagatt
     9241 tccatgaaca atcgcccaat atttgcggag agacttttta tctttgagtt cttgggcaag
     9301 tactagatgc gcatcatcgt ttttagcaat catgagaaga cctgacgtat ccttatcaat
     9361 acggtgaaca atccctggac gcagaacccc attgataccc gacaagtcct taatatgata
     9421 catgagggca ttgactaggg ttccactggt atgaccagca ctcgggtgca caaccattcc
     9481 ctgaggtttg ttaacgacag ccacatcctc atcttggtag actatttcta gcggaagatc
     9541 ctcagccaca tactctaata cctctggttc tggcacatgg taagtgacga catcaccctc
     9601 ttggactgtg tatttagctt tcttgacttg accattgacc aagacctggc ctgatttaat
     9661 ttgttcattc gcgagactac gtgataattc tgacaaatct gacaaagcct tatccaaacg
     9721 cagaccacca gtttcaattt taatttccat ttatttcctc ttttagcatt gcaatcaata
     9781 aaataatcac tccaaccgtc agatagctat ctgccacatt gaaaattgca aagttgataa
     9841 agtcaaggtg gaacatatcc acaacaaagc cctgactgac cctgtcaata aagtttccaa
     9901 gaccacccgc gattattaga gtcaaaccca agaccatcca gaatgagtcc tccatgtgtt
     9961 tatgtaaata ccaaatggca cctatcacga caaccagagt aatgacagcg aataacagct
    10021 gctgatcttg taagatagaa aaggctgcac ctcgattttg caggtaggtc aagctaacga
    10081 aattgggaat ccaggagcgc acttcaccca gtggaatctg ctggacgata taggatttga
    10141 ccaactgatc cagcccaatt aaaagcagta caatgactgc cactattgct ctttttttca
    10201 tgatttcctc ttttgatcaa aatattcttg catgacttct acgaagagag tcccagcttg
    10261 actaagctcc acttcttcac gtttaacata gaccatgcgg ttatctaggt tatccttgag
    10321 acgaataact gtaatgccat taacactgtc actatctaaa aatccagaac ctgtcgcata
    10381 ggcgtccgtc cgctccaaaa taccattcaa ggtggcacgg tctgtcacat taaacatctg
    10441 tgagctagcg ctggtatcga caaagttctc tgaataataa aggtactcgt ctttctcttg
    10501 agtgaaacga accgttggta aatccgctaa atcctccatg actaattcct ctttctgggc
    10561 taaaggatga ccctcacgga gataaatatg ggtatggaaa ggaatcaatt cgatgacctc
    10621 cagacctaat ttttcaaccc gttgcataat ccccttttta ttttgattgt tgaggtagat
    10681 aatcccaatc tcactatgcc cttgcgccac ttcatctaat atttgaacag tagttgattc
    10741 aaaaatacgg aagttcttat agtcaggata gcgctctgaa aaggccgtaa tagttggtgg
    10801 caagaagtca tagtgctggc tagcaacgga aaattcatct ttttcttctt caggattggc
    10861 atactgattt tgaaaaatat caaatccttt aaccaattct tgcgcttttt cataaaattc
    10921 catcccacga cgggtcaaga aagtccctga gctggtccga cggaaaatct taaagcccaa
    10981 ctctttttcc aaatcacgaa cagaaataga cagactcggc tgactaacat acatcttttc
    11041 agcagcttca cgaaaagtac cactattggc aatagccaca acatagcgta attgttgaat
    11101 gttcatcttc tacccccaac ttctttatct tttcattata ccatatttta gaagttttcc
    11161 aaaaaggaaa aaagaacatc ctattcttct taactatctt cactatctgc ctttcttacg
    11221 ccaatcttat tttcaaaatc aattcaaaaa aataagtggc tacacaccac ttcagtatcg
    11281 gaaagaaaaa cgttgactat ttgtgaaaaa agaaaatgcc ggaaaattcc gacatttttt
    11341 tagtaagcta acttcctgaa aataggagta accaaacatc ccagaaggca gtgatattga
    11401 tgagaatatg aactagtatt ggatagtaga gatttttagt catgcgatac aatagagcta
    11461 aaataagacc tccactagca taaatgaaaa aagcaagagg agttaaagca aaattgatat
    11521 gaatataacc gaaaataata gcagaaagca aaacatctcc gtaccaaggt gagtttttga
    11581 aaaaggttgt cataagcaca cctcgataaa tcaattcctc agcaataggg gcgatgaagc
    11641 aaacgatgag caagaaataa gggaactcct gtctccccat catttctacc gtttcattca
    11701 aagaaatctg atttgaagac agagatatga aatacgaaaa gagaaagtca gacatatacg
    11761 aagtgatgta acccagtaaa aggtagataa aatacctcag ctgccacttg aaatgaaaaa
    11821 ttttctttcc tgcaaaaacc agtagataga tgaccgctaa taaaagaact ccactctcca
    11881 tcaaaagcag aatctgaaaa aattcacgac ttgctggtag atagggcttt gcgagataat
    11941 tgaaaagcct ccaaaaccaa gttgatttgt aaaaaattaa ggacaatact agtaaaattt
    12001 gaatagctcg ttttttcata ttaaatcctc tgttttctcc ccttgtttct tcatattcct
    12061 aataaatttt acttaaaatc tcgcagcact tcctttgcaa agatgattac ctcctccaat
    12121 atatcctaaa tcatcggttc ctctgggaca aaatccatga accggcatag ttaatactgg
    12181 ttccatttgc caaggataat aaccattagg ttctttacca tagggatctg ctccaccttg
    12241 tatcagctct aattcttcat ttgttagatt tatatttttt gtcattaaaa tcatgatata
    12301 tttcctccat tattcttcag attagttgag taatctgata ctcataccta cttacaaaaa
    12361 aactattata ttaagttggt tttttaattt gtctagttgc aacattgaca aacatagtat
    12421 agcatatctt tgccaaatat cctcttcaaa tcatgaattg tcatcaaaac atcttaaact
    12481 ataaaatcaa ttagtctcaa gctttctatc aatttcttct ccaaaatatg ctataataat
    12541 agcaaaagat aaagaaggaa gacctatgat taaactacta gccttggata tggacggcac
    12601 cctcctcaat gaagccaagg aaatcccaca agctcacatt actgctattc acaaagccat
    12661 tgaaaaaggt gtcaaactgg ttctctgtac gggtcgcccc cttttcggtg tcctccccta
    12721 ctacaaaaaa ctgggactcg acctccagaa cgaatatatt attgttaaca acggttgttc
    12781 aactcaccag accagtgatt ggagtctagt tgactggaaa gaactgagtc cagctgacat
    12841 cgaatacctc tatgaccttg ctgaaaagag tgatgttcag ttgacacttt ttgacgagtc
    12901 acattatttt gttctcggtg gcaaacccaa tcaagttatt gaaaatgatg ctaaactagt
    12961 attttcagac ctgactgaaa tttctcttga agaagcgact agtggaaagt tccggatgtt
    13021 ccaaggtatg tttttaggaa caaaagaaca aacagacgat tttgagcagc gttttgctga
    13081 agagctttgc caacgattca gtggagttcg ttcgcagcct gtcatttatg aagcaatgcc
    13141 gcttggaacg acaaaggcta ctgctctttc acgactagct gagattttga agattgattc
    13201 ctcagagatt atggccatgg gcgatgctaa taacgatatc gaaatgctcc agtttgcagg
    13261 gcttgggatt gcaatgggaa atgccagcga ttatgtcaaa tctcttgcgg atgccgttac
    13321 ctcaagcaac gaagaagacg gcgttgcgcg tgctattgag aaatatattc tataaaaaag
    13381 aaaagcaaat agacagaagt tactgctatt tgcttttttg ctaatatttt aaaatacgga
    13441 ctaatcaata gacaagaata gcgaatcata aaaatcgatt tactagatgc catagtatct
    13501 tatagttgct aataagaagt tagtctagca gaatgaatct ctcatttctc ccacactaat
    13561 ctgtaatttg tctatacatt tctggtcttc tatctcgaaa caagccccag tttaggcgtt
    13621 cacttgctcc cttgtctagg tcataagtag ctaacagaac agcttcttct tgtctttcag
    13681 ctcgttctag aatagctcct gtttcatccg tcataaagga ggaaccgtag aagtcaagac
    13741 tggaactctg tccgccattt tcctcactag gagtaacctc ctctaaacca taacgattgg
    13801 ctgcgatgac tggaacaata ttcgctgctg cgtgcccttg catagtacgt tgccagtgac
    13861 cacaactatc tgtatccaaa attggctctg aaccgatagc tgtaggataa aagagcaatt
    13921 cagcaccatt caatgcaaga cagcgcgctg tttcagggaa ccattgatcc caacagatac
    13981 cgataccaat cttagcatag cgagtattcc agaccttgaa accagtgtta ccaggcgtga
    14041 aatagaattt ttcttgataa taatggtcat ctggtatgtg ggtctttcga taaacgccca
    14101 gcacttcccc atctgcatca atgacggcaa tagagttata caagacatta ccatcttttt
    14161 catagaaact gattggtaaa acaacttgta gttccttagc aatcacctta aaatgctgaa
    14221 tggcagtatt ttccgctaca gattgggcat actggtagta gtcatactga cgttcctgac
    14281 agaaataggg atgttcaaac aactcgggca agagaataat ttgggctcct tgctcagcag
    14341 cctgacgtac taaacgctct gcggtttgga tatttgttgc cacatcctta gcgcattgca
    14401 tctgaatggt tgcaactctt acatttctca tctttttctc ctattctgga atttgttggg
    14461 tgatacagtg gatattgcca ccacctaaga gaatatctct ggctggtatt ccgacaactt
    14521 tacggtctgg gaaacacttg ctgaggatat ctaaggccac ttggtcgttt acatcctcaa
    14581 actgtggaac caagacagcc ttgttggcga tataaaagtt tacgtaggaa gctgctagtc
    14641 gttcacctgc gtatcgcttt tcttctcctt cttcatagat gtagcctggc aaatcttctt
    14701 ctgtcacaac ttgtcgaact gcagggatag gcaatttatg aatggtgaag tgacaacctt
    14761 ttgcatctgt ttcctgttct aagagttcga gatctgcttt tgacatggca tactggggat
    14821 cattttcgtc atctgtccaa gctaaaacaa cctcagcagg accaacaaag gcagcaacat
    14881 tatcgacgtg ttcattggtt tcatcctgat aaataccata aggaagccaa ataacttttt
    14941 cagcaccaag actttctaat aatgtgtttt caatctcctc tttagtcagg ttaggattgc
    15001 gaccaggact aagcaagcaa ctttcagtta cgagaatagt tccttgacca tcgctatgga
    15061 ttgcgcctcc ttccagtaca aaaggtttag catcatagac aggctttccc aaggcctcag
    15121 caaaacgact ggctacttgg tcatcctctt cataatcttg ataaagacca tcataggtgc
    15181 ctccccaagc attgaaggcc caatccacgg ctaatttctt acctttatca ttgacgagaa
    15241 tggttgggcc agtatcacgc gcccaggcat cattggtggg aatgtctaaa taaacaacct
    15301 tgtctccaag ataggattgg gcttcagata gataggcctg ctccaccaaa agatagactc
    15361 tttccccttc tgctatggtc tcgatgatct gagtaaatga tcttttagca gcctttcctt
    15421 gaaaaggcca tgatcctggt cgagtcggcc atatcatgag ggtaccatga tggggttcgt
    15481 actctgctgg catgtgatag cctaattttt ttggactgtc catcatgata atctcccttt
    15541 aaagtcttga tagccaaaag ctttgagtaa gctacagtct ccctgttcgt ccatgagata
    15601 gagacttggc aatccaatac cattaaaggt attatttttg acaaaagaat aaatggccat
    15661 gtcttgaaaa taaagtctgt ctccgatttg gactggattt tcaaaactat aatcaccaat
    15721 cacatcgccc gtcagacagg tattagaaga aagtctgtag gtatgggctt tttcctgtgc
    15781 ctcaaagcca tttctcaaag gtggacgata gggcatctca agtacatcag gcatatggca
    15841 ggtcgcagag gcgtctaaaa ccaagatttc cataccgttt tctacaatat ctaatacctc
    15901 agttgctaaa taacccgcat taagcgcaat ggcttcacca ggctcgatat agatttcaag
    15961 attgtaagtt tttcggatac gcttgatttc tgaaatcagc aaatccacat cgtaaccttc
    16021 tcttgtaata tgatgaccac cacccatatt gagccatttt acctcatgta agtagggacc
    16081 aaattgttct tctactgctt tcaaagttgt ttgtaaatca tctgctccct gctcgcaaag
    16141 ggtatgaaaa tgaagtccgt caaccaaatc tagcaaatca ctcggaatct tgtctatagt
    16201 aactccaaag cgagaacctg gtgcacaagg gtcatagagc gcgtgatctc cttgagttga
    16261 acactgaggg ttgaggcgca aaccaacact gacaccagcc tctcgacaac gagccccatg
    16321 cttacgcaac tgtctctctg agttaaagac gatatggtct gttatcccaa gcaattcctc
    16381 caagtctgca tccttgaaag caggcgcaaa tacatggact tcaccaggaa attcttccct
    16441 tgccaatttg gcctcataga gtccactagc tgtcgtacct gatagatact ggctaatcaa
    16501 gggataagtt ttgtagaggg aatatgcctt ctgggcaagc aagaccttgc aaccggcctc
    16561 ttcttgtaca tattgtagaa tgcggcaatt agcttctaac ttggccaagt caataacata
    16621 ggctggtgtt ggtacttgtt ctaacttcat tagtccacca tttgtggatt ttcaaccaca
    16681 acccatggca aaccatactc attcaaagct tccatgaatg gatctggatc taactcctca
    16741 aggttataca ctccagcttg tttccaagtt ccgttcatga ctaattttgt cccaatcatg
    16801 gctggaacgc ctgtcgtgta tgaaattgct tgtgacccga cttctgcata gcactcttga
    16861 tggtcgcaaa cattgtagat gtagatagtc ttttcaacgc catctttgac acctgtaaag
    16921 atacatccaa tattggtttt tccgactgta cgtgggccaa ggctagcagg atctggaagc
    16981 aaggctttca aaaattgaat tggaacaatt tcttggccgt taaagttaat ggtatccgta
    17041 cgaaggagtc caacattttc aagacatttc atgtgcgtca agtaagattg accaaaagtc
    17101 ataaagaaac gaatgcgttt gacacctgga atgttcttgg ccaatgattc gatttcttca
    17161 tggtgaagga gatacatatc tttttgtcca acttgaggga aatcatactc acgcttgata
    17221 gacatagctt cgacttcgac ccatttccca tcttcccagt aagaacctgg cgcagaaacc
    17281 tcacggagat taatttctgg attaaagttg gttgcaaatg gataaccgtg gtcaccgcca
    17341 ttacagtcta aaatgtcgat ataatggatt tcatcaaaat agtgtttgag ggcataagct
    17401 gaaaagacac tagttacacc tgggtcaaaa ccagaaccaa gaagagcagt caagcctgct
    17461 tctttgaatt tctcttgata agcccactgc catgagtagt caaagtaggc tgtaaaacca
    17521 agttccttac aacgtttttc gtagatagca cgccactcag ggtcttctgt gtcttctgct
    17581 tcgtagttgg ctgtatcgat atagtgaaca cctgttgcca aacaagcatc cataatggtt
    17641 aaatcttgat aaggcagagc tacattcaaa acagcttctg gtttgtagct ttcaatcaag
    17701 gtaatgacct cttctacctt gtcagcatca agtgctgcag tttcaatttt agtacttgtt
    17761 ttgccttcta gcttcgcttt caagtcatcg cattttgact tggtacggct agcaatcata
    17821 atctctgtaa atgtttcgct atcttgacaa atttttgaaa tagcaacttg ggcaacgccc
    17881 ccacaaccaa taactagtaa acgactcatt tttttccttc ctcttcttct aaaatgtcct
    17941 caacatactt gggcaacata aaagctccca cgtgtaagtt tgcagtgtag tattctgtga
    18001 aaagctggcg ttttttccaa ccttccttgt caaaatcttt gacagggtgg tattttttcg
    18061 atgcaaatcc aaacaaccaa tagccagctg ggctagttgg aatatgggcc tgataaaccc
    18121 gactgattgg aaaggattga ttgaccttgc ggtgcatgct tcggcaggcc gactcatcct
    18181 cgtcaaagaa gggactccca tgctggtaaa tcatgatgcc gtcttccttc agagctcgat
    18241 aactattgcc gtagaattcc ttggtaaaga gtccttccgt atggccaaat ggatctgtcg
    18301 catcgttgat gataatatcg taatcatctt cgcagtttcg caaaaagcgt agcccatttt
    18361 ggtagtaaat ggtaacacga ggatcatcta gccctgcagc aaagtctggg aaatactcac
    18421 gacagacctc gaccaacatc tcatccggtt ccacgatatc gatttgttcc agttcaggat
    18481 agagtgttaa tacttgggca acaccgccgt caccaccccc aataaccaat actttctttg
    18541 gatttgggtg gacagccatg ggaacgtgaa cggtcatttc attgtagacg aaatcatccg
    18601 catctgagaa caagacatgg ccatttaaaa tcagtatttt cccaaaagct ggcgtatcca
    18661 agacttcgat atcctgccat tcactttttc cagcgtaaag ttgcttggct gttctcagag
    18721 acaatttgac atctggagta tgaacttcag aaaaccataa atccatctat ttctccttat
    18781 attatattta gcgcagttag ctactgccaa agcccaagtg gtatacttgg aataagccac
    18841 tgtggattag ttcatttcct ccaatcgctt gacgatttcc gaaaggctct aaccacagct
    18901 tttgtttcaa ttggtaatga gtttgataac gcatagacgt tttatctcgt ctaaggtaac
    18961 aaggcaaaaa gttcgtggat aaccaagtag acttgaaata ctacatctgg aggtactccc
    19021 atgctttatg tgggcattga tatcgctaaa aataaacacg atgttacagc cttgaatgtt
    19081 caaggaaaaa ctgttcttaa accactcact ttttcaaata ataaagctgg ttttgaactc
    19141 ttagatctgt ctcttcgaca gctcaaccaa gactgtctca tcgctcttga agatactgga
    19201 cattatgcct ttaatctact gaacttcctg catgaacaag gatataaagt ttacacctac
    19261 aatcctttgc tcatcaagga atttgccaaa tcattatcac ttcgcaagac taaaacggat
    19321 aagaaagacg ctcatggcat cgctcttaaa cttctttctg acccaatcgt gaacaatttc
    19381 aacacgataa tcggcaagta gaactaaaaa tactggctag acatattcat cgtctcaaga
    19441 aaaacagtct gattggaaag tacaatacac tcgttgtctt gatatcatct ttcctgagtt
    19501 ggataaaatc gttggaaagc attcagaata tacctaccaa ctcttgacac gctaccctaa
    19561 tcctcagaaa aggattgagg caggatttga taagctgata gaaattaagc gattgaccgc
    19621 ttctaattca agggcttttt gccgtgctct aaaatcatcg ccaaatcatc aaattgaaat
    19681 aagaaatagt ttagataaaa ctattgactt ttcttatagt ttgtctttct cttaataacg
    19741 ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg ttccttggcg
    19801 aattggatat agtctacaat ttctcgtgta atgcgttcac caggagccaa gataggaatc
    19861 cctggaggat agcacatgac aaattctcca cagacctgtc caacagactc atctaaggtc
    19921 aaactttttc tctctgaata gaaggcttct tgtggagaca gcaccaactc gggctgaata
    19981 tattctccag ctattaagtc cttcccatct cgtgaataga gtctcttaat atcagccaga
    20041 gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata ggccaagata
    20101 ttgccgatat caccaaactc gatctgaatg tcgtattcgt ctcgcaagag gtcataaacc
    20161 tcgatacctg ttaagcctat tccctgagtg taaactgaca acttggttac atcaaaatca
    20221 cagaccgaca caccatctat taactctttt gagtaagcat agtaaccgcc aatggcattg
    20281 atttcatgac gagcgtactc ggatagctca atgacttcct caaacgactc tttaccacga
    20341 agggccaagt tgcgacgtga aatatccaaa ctagccatca ataagtaaga ggcagatgta
    20401 gactgggtca ggtttataat ctgacgaacg tactcgggat tcatctgctc cccgattaaa
    20461 agaagcgaac tttgtgtcaa actcccacca gacttatgca tggaaactgc tgccatatca
    20521 gctcctgcat ccatagcaga aattggaagt ttaccagtga aatgcaaatg cgctccatgg
    20581 gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa cccctttagg
    20641 tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc atctggatgg
    20701 tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc aatcttagga
    20761 tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc attgatagca
    20821 gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt tgccagaatc
    20881 atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct agctccaaaa
    20941 gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc taaattatcc
    21001 aagggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag ttcgacaagt
    21061 tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat ccttttcttg
    21121 cgtaacttca ccaaggcttc ataaattggg gcttggtttt gatctaactc tttcaaaaca
    21181 tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt tgactcatga
    21241 aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt ggtttctgtt
    21301 gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg tactttattg
    21361 atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat taggacaagt
    21421 gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg gcttttcaac
    21481 cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt ttatatttgc
    21541 tagaaaacat catctcattt tctaagcttt attactatat catgttttta agaacttgta
    21601 aagtattttt tgataaaaaa caaaaatata gtagattgaa actagaatag tacacttctg
    21661 cttctaaaac attattagaa atagatttta ctgtcctgat cgatttgttc tgttcttgtt
    21721 tcattttact atatgttcgt aaatgaaagt tctataatga agttagccac ccagccctcc
    21781 taaaaattag atggttcaac tagctgatag ctcttgtagg cataagaaaa cacctctgtg
    21841 ttatacttgt tgttcaacca caaacacaag aaaggcacag agatgcaaga caattatact
    21901 acaaaagcca aacatttgac aatcgatagc cgtcgcttaa tcgaaagatg gaaaaaagaa
    21961 gggaaatcaa atagagaaat tgcctctctg cttggaaaag ttcctcaaac tatccacact
    22021 gaaatcaagc gtgggacagt ccgacaatgt cttggaaaag ggcgcttcaa agaggtttat
    22081 tctgccgact atgctcaaca gtcttatgaa aacaatcgca agcgctcggt caggaaatca
    22141 agcgtgacca aggaactaaa ggaaaagatt ctccactatc ataaccaaaa attttcgcct
    22201 gaaatgatgg tgatggttaa aggggttaac gtgggaattt caaccattta ctattggatt
    22261 catcatggaa aattggggtt aagcaaacag gatctgcttt accctagaaa aggaaaagcg
    22321 cttaagaaac aggctagcac caactttaaa cctgctggtc aatccatcga acagcggtct
    22381 gaagctatca atcttcgctt ggagaatggg cattatgaga ttgatacggt tctacttacg
    22441 agagcgaaaa actactgttt gcttgtcttg actgatcgaa agagtagaca tcagatcatc
    22501 cgattgattc caaataaaag tgctgatgtg gtcaatcagg ctctaaaact catcttaaaa
    22561 caacacaaga ttctttccat cacggcagat aatggaacgg aattcaatcg cttatttgat
    22621 gtattttctg aggagcacat ctattatgcg cacccctatg cctcttggga aaggggaact
    22681 aatgagaatc acaacaggct cattcgtaga tggttaccta agggaatcaa gaaaatgact
    22741 cccaaagaag tcgcattcat cgaaaagtgg attaacaact atcctaaaaa atgcttggac
    22801 tacaagtcac ccagagaaga cttctggatg gctaacttga acttgaaatt tagcgtcaga
    22861 aacaaatcac gaaattgatt caaattttgc aggtcgttta aatatcctgc gtgcgggtgt
    22921 tcttgatgct aacgatggaa ttatttccat tgctggtgtg gttatcggag ttgccagtgc
    22981 cacgagcaat atctggatta tctttttatc aggttttgcg gctatcttag ctggtgcctt
    23041 ttcaatggct ggtggagaat atgtatccgt ttcaactcca aaagataccg aggaagctgc
    23101 cgtttcgcga gaaaaactct tgctagacca agatagggaa ctagccaaaa aatccctcta
    23161 tgcttcttat atccaaaatg gagaattcaa aacttctgcc caactcttga ctaataagat
    23221 ctttcttaaa aatccactca aggctctggt agaggaaaaa tatgggattg agtatgaaga
    23281 atttaccaat ccttggcacg ctgccatttc tagcttcgtt gcctttttcc ttagaagttt
    23341 gcctccaatg ctgtcagtga ccatttttcc aagtgattac cgcatccctg ctaccgtcct
    23401 tattgtcggt gtggcccttc ttctcactgg ttacacaagt gctagacttg gaaaagcccc
    23461 aaccaaaaca gctatgattc ggaaccttgc tattggtctc ttgaccatgg gagttacctt
    23521 cctgctcgaa caacttttca gcatttaaaa tacaagaaat acctcgattt tgaagtcgag
    23581 gtatcttttt tacatttgca caatcttgcg ataacttctt gaagtaatca tgaaaatcag
    23641 cacataggcg atgaggaaga tagcgcagat agacaaggtc acaatcaaca tcatagtcgt
    23701 atccagtaca ccaatcactt ttaaaatcag gctaagcata tggtaggcaa aggcgagatg
    23761 tatgaaggca aagagcaaag gaaggaagaa aacagttaaa acctgtttgt ggatggtttg
    23821 cttgatttgc ttttggtcca aaccgacttt ctgcaagata ataaagcgtt cacggtcttc
    23881 gtagccttca gaaatttgtt tgtagtagat gaccagaaca gttccgacca taaagataat
    23941 ggataggaaa ataccgataa agaagacacc accaaagagg gcactcatct gagcactagc
    24001 atctgctaga ttgctaccat aaacatagct accttctgtg tctaattgag cattaaattg
    24061 attgaggtag ttttcatact cctcagcgac cttgagttgt tcttcttcgc tggcatctac
    24121 attcatacca ccgtaaaact gattatagat agccgaatct gggaattgat ccaaaaaggc
    24181 ttgtaaatca ggaacaacaa ggtaattgta atcagtagtc aagatattaa acttatttgg
    24241 aacatggttg acaatgaaat ctttattaaa ttcttctttg acagaaaatt gatgatcatt
    24301 tagagttaga gctttctgtc ctttcagtcc gtcatttttg gcaaagagac cgacctcatt
    24361 tcctgataga gacagttttt gaccagtcat attttcataa tctttttggt caaataccat
    24421 gaaaactgtt tttggttgga cacggttttg tcctttttca aaaatagtta acttggttcc
    24481 ttcttgattt gcaataccaa agttactgta acgaagtact tctttctctt tgacactata
    24541 acctttgtca cttgcaaact ggctcaagag tttgtccaaa tcttcttttt caacattttg
    24601 ccctgaaacc ccaaaatcat gaggatttag aactttttta aaggattctg cggaattgaa
    24661 aatgcttgtc gctgctgaca tggttaccaa aaccattgtt gacaaaatag cgatggttgc
    24721 tagtccaacc gcatttttct tcatacggaa aatcaagttg gaaacagata tgaggttatt
    24781 aggttggtaa tagtatttct tgtttttctt taagatttgt aggaagactg taatccctgc
    24841 attaaacaat agataagtac caaagataac cagcaaaaca gctaggaaga aggttgttag
    24901 ggctgtaaga ggatcggtta ccgtaagggc aagataatag ccaatcccta aacttatgga
    24961 accaagaatc gtttggagag gaaggaagcg accttttttc tctccgctag ctttctcacg
    25021 tgagagctgg agggcattca tacgggcgat tcgaagggca ttcaggaaca tgaggcctag
    25081 gaaaatcaat ccaaagacaa gaagtactgt aataacaact ttcatctgga aggtagcgac
    25141 cagttccacc ttcaatttca tcagtttgag taggaaagcg aaaatcaact tgtcaaacaa
    25201 ggctccaata ccgattcctg ctccaacagt tagaatccca aatagcacta actccttaaa
    25261 ggtcatactg attagatggc gcttctccaa gcctaacatg ccatatatac ccagttcctt
    25321 ggaacggttt ttcatgacaa aactattggc atagaggacg ataatggctg acgcaagggt
    25381 aacgacaaac ataccaaatc caagtgttgc ttgaatggtg gttcctccac ggatttccgc
    25441 aatctttgga ttgaaggtta gggagtaaaa gagataggtg atggtgactg ccaagagaac
    25501 agccagtgca aagggatagt agagtttgcg gtttttaatc aagttcgata ccgctaactt
    25561 attggttaat cgaaacatac taattcacct cgcttgccat gacagtcaag gtatcagaga
    25621 tttcttggaa catctgacgc tctgtcttct ctccacggta gatttggttg taaagaatgc
    25681 cgtctttgat aaagagaaca cgcttggccc tgctagcagc tgctgttgag tgggttacca
    25741 tgaggatggt ttgcccacgc tcattgattt cattaaagac atcaagtaag gctgcagatg
    25801 acttggaatc aagggctcct gttggctcgt ccgcaaggag aatttcaggt tctgtgatga
    25861 tggcgcgggc tactgctaca cgctgtttct gaccaccaga aatctcgtaa gggtacttct
    25921 cttgcaattg gttaataccc agattctcag ctgtcaccac caatttcttc atcatctccg
    25981 ttataggtct tcttgacaag acaagcggaa gcaagatatt gtccttaaca gacagagtat
    26041 ctagcaagtt aaagtcttgg aagacaaatc ctagcttttc acgccggaaa ctagaagcct
    26101 gtgaattttt aatagttgcg gtgtcagttc cattcaagta aacctgacca cgacttggtt
    26161 tatccaacat agctagaata ttgagaagag ttgatttacc agaaccagac tcacccatga
    26221 tggcaacgta gtcacccttt tctacggtaa agtgaatatc cttgagggct tctacttggt
    26281 tgccctgaaa acgtgtttta taaatttttt gaacgtgttt tacatctaaa agtgtcatga
    26341 aaatctcctt tcttaatatc ccatcaaatg aaatgtagct tgcatcatag tgcatcccag
    26401 ctgaacgcgc tcaatttctt tgtgacttgg ttttctagtt ttcttatgta agatttgttt
    26461 catgatgtta ctcctttgtt ctttatgagt ctagtttaca tcaaaaaaag accgcttgcc
    26521 ataacctaac cttacaaaag agacttta
//
LOCUS       DAVMHG010000011        26498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMHG010000011 DAVMHG010000000
VERSION     DAVMHG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.011"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            112..288
                     /locus_tag="VYR08_000386"
                     /pseudo
     CDS             112..288
                     /locus_tag="VYR08_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            704..1093
                     /locus_tag="VYR08_000387"
     CDS             704..1093
                     /locus_tag="VYR08_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV3465970.1"
                     /translation="MKAQAIVTSQGRIVSLDIAVNYCHDMKLFKMSRRNIGQAGKILA
                     DSGYQGLMKICPQAQTSRKSSKLKPLTAEDKAYNHALSKERSKVENIFAKVKTFKMFS
                     TTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(1145..1507)
                     /locus_tag="VYR08_000388"
     CDS             complement(1145..1507)
                     /locus_tag="VYR08_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465971.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1508..1717)
                     /locus_tag="VYR08_000389"
     CDS             complement(1508..1717)
                     /locus_tag="VYR08_000389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465972.1"
                     /translation="MKQKNYLVSNATVIQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            complement(1869..3749)
                     /locus_tag="VYR08_000390"
     CDS             complement(1869..3749)
                     /locus_tag="VYR08_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3465973.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQSYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLIAIEANKACRANDIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSSINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3743..4612)
                     /locus_tag="VYR08_000391"
     CDS             complement(3743..4612)
                     /locus_tag="VYR08_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3465974.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALISYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4696..7341)
                     /locus_tag="VYR08_000392"
     CDS             complement(4696..7341)
                     /locus_tag="VYR08_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV3465975.1"
                     /translation="MENIVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7432..8712)
                     /locus_tag="VYR08_000393"
     CDS             complement(7432..8712)
                     /locus_tag="VYR08_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV3465976.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8883..10967
                     /locus_tag="VYR08_000394"
     CDS             8883..10967
                     /locus_tag="VYR08_000394"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV3465977.1"
                     /translation="MKPLLETIDTRLGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFQPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(11007..12686)
                     /locus_tag="VYR08_000395"
     CDS             complement(11007..12686)
                     /locus_tag="VYR08_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV3465978.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIHWIGNERGH
                     AGDPLWQKMNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMIIEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13442..14671
                     /gene="arcA"
                     /locus_tag="VYR08_000396"
     CDS             13442..14671
                     /gene="arcA"
                     /locus_tag="VYR08_000396"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV3465979.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14729..15745
                     /gene="argF"
                     /locus_tag="VYR08_000397"
     CDS             14729..15745
                     /gene="argF"
                     /locus_tag="VYR08_000397"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV3465980.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15910..16857
                     /gene="arcC"
                     /locus_tag="VYR08_000398"
     CDS             15910..16857
                     /gene="arcC"
                     /locus_tag="VYR08_000398"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV3465981.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17068..18579
                     /locus_tag="VYR08_000399"
     CDS             17068..18579
                     /locus_tag="VYR08_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV3465982.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     IIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18601..19932
                     /locus_tag="VYR08_000400"
     CDS             18601..19932
                     /locus_tag="VYR08_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV3465983.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            20027..20154
                     /locus_tag="VYR08_000401"
                     /pseudo
     CDS             20027..20154
                     /locus_tag="VYR08_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(20334..20627)
                     /locus_tag="VYR08_000402"
     CDS             complement(20334..20627)
                     /locus_tag="VYR08_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465984.1"
                     /translation="MGVLGYYEGFVPYVSNQYKNQAEEEDKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQIS"
     gene            complement(20615..20866)
                     /locus_tag="VYR08_000403"
     CDS             complement(20615..20866)
                     /locus_tag="VYR08_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3465985.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEY"
     gene            21070..21147
                     /locus_tag="VYR08_000404"
                     /pseudo
     CDS             21070..21147
                     /locus_tag="VYR08_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21264..22148)
                     /locus_tag="VYR08_000405"
     CDS             complement(21264..22148)
                     /locus_tag="VYR08_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3465986.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(22293..23444)
                     /locus_tag="VYR08_000406"
     CDS             complement(22293..23444)
                     /locus_tag="VYR08_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV3465987.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(23590..25356)
                     /locus_tag="VYR08_000407"
     CDS             complement(23590..25356)
                     /locus_tag="VYR08_000407"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV3465988.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATLDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(25369..26097)
                     /locus_tag="VYR08_000408"
     CDS             complement(25369..26097)
                     /locus_tag="VYR08_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV3465989.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(26112..>26498)
                     /locus_tag="VYR08_000409"
     CDS             complement(26112..>26498)
                     /locus_tag="VYR08_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV3465990.1"
                     /translation="MDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSNAQDANQA
                     KKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTNNSYDQPS
                     LNFDQKNHMVTITINSNGNLEFELHF"
BASE COUNT         7611 a         5895 c         5227 g         7765 t
ORIGIN      
        1 tgggttaaaa aagaaattaa ctgagtaaaa actctcgaaa ttcaattcgc tatattatca
       61 ctcaacttga acaaatacca gaaccacaaa aaacagatgt aaaggaaaaa tatggactta
      121 aaatttgaag gggttgattt agaatacaaa aaagctaaaa acaatcttcc agagtctttc
      181 tgggaaacct actcagcatt tgcaaataca aatggcggga aaataattct cgggatagat
      241 gaaaaaaaca ttgacaccta ccaaagagtc aatagactcc ctgcgaaaca aaattatgaa
      301 gcaagtaaac aactaactga tgcacgattt aagagtcttg ttggtgttca gcgtaccact
      361 tttgaagaga tgttagctgt attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga
      421 cgaaaaccta aattaagcct agaagacctt cttatggcca ctcttcaata tgtgcgagaa
      481 tatcgaactt atgaagaaat tgcggctgat tttggtatcc acgaaagcaa cttactccgt
      541 cggagccaat gggttgaagt aactcttgtt caaagtggtg ttacgatttc aagaactcct
      601 ctcagttctg aggacacggt aatgattgat gcgacggaag taaaaatcaa tcgccctaaa
      661 aaaagaatta gcgaatgatt ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat
      721 tgtcacaagt caagggagaa ttgtttcttt ggatatcgct gtgaactatt gtcatgatat
      781 gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggaaaaatct tggctgacag
      841 tggttatcaa gggctcatga agatatgtcc tcaagcacaa acttcacgta aatccagcaa
      901 actcaagcca ctaacagctg aagataaagc ctataaccat gcgctatcta aggagagaag
      961 caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg
     1021 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga
     1081 actaggattc tagttttgca ggaagtctat tgaaatacta agcctacaca gactgatttc
     1141 aattttacca ttcttcatag tcaaaaccat cataatctgg atattgttct ggcgacgttc
     1201 caaccgaacg tacgagataa gcttcttcgt cctctgtctc gatttctctc agcacttggc
     1261 attcaaaacg ccattcatca ccgaagtcga aaataaactt aaatttttgt ttgacactta
     1321 ggctatccag atagacattt tctgtgtaac gttcttcaac atcgtcacta acaaagctaa
     1381 gaaagtaaga atctgcatga ctccactcaa cattatccat gaaaaatgcg tgagcatgat
     1441 catttgcaaa atcaaaactc cataaaatca catctgctag ttccgctaac gtttttgttt
     1501 ttggaatcta tatatgtcgg tagcaacctt ttttaaattt gacggaaatg atccaagctt
     1561 ctacagatgc tggcttttca gcttctattt cttgttttac taattttaag tgtagcataa
     1621 tacctccaat tgctcttagg aaacattcac tttctgctat tttatcataa gtttgtataa
     1681 ctgtagcatt tgaaaccaga taattcttct gtttcacgcg aaacaccctg cgccaagcaa
     1741 ggtgttcttg tttttatact caatgaaaat caaagagcaa actaggaagc tagctgcagg
     1801 ttgctcaaaa cactgttttg aggttgcaga tggaagctga cgtggtttga agagattttc
     1861 gaagaatatt aagtcgtata aatcgtcgac gctgtcgcaa tggtatgcca ctggttggct
     1921 gtagttgctg cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc
     1981 agctcgtcga tgcggtcaag ctgaccagcc agataaacct cgatacggct ttctgctcgt
     2041 ttgatgcgtt gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg
     2101 ttttctttca gccattggtg actaaagagg gcatggaagt cttcaatttg gcttctaagt
     2161 tctggtaatt cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggca
     2221 tgacgaatgc gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga
     2281 gtttcaaaga gataagcata gtttccagct ttttctttaa tgttagcaag cgtctcagca
     2341 gcctgagcga agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag aatcggacaa
     2401 agaatatcct gataaaaaac atagcggttg ggattgatac tgctgagatt gcctggtagg
     2461 tctggtaaga ggttggcaag gtcaatctgc ataaaatcct caaccgttag accagtattg
     2521 gtcttgaagt gcgcagacaa accatctagg tcattgcgat agctgagttc tgcccagatt
     2581 tgcaagcttg gtaggataga gaactgggct gtttccccac cgttatcccc ccaacccgtc
     2641 acgatgactt ctttgatgtc attggcacga caggctttat tggcctcgat agcgatgaga
     2701 cggctgaaat ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca
     2761 aggtcatggc taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc
     2821 tgataataat cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga
     2881 gtttcctctg gaatttccac atcacggtcg tactggccat ccgctgacat gagtttgaag
     2941 aacatatcac tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc
     3001 tccaagtgtt ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt
     3061 cccaaaccaa ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta
     3121 gacaacgtgg caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga
     3181 atgtcctcta catcacggag ctcctgcact tccttgacac cccatttgac aaaggctgac
     3241 aggtgggcca aggtctggat gcatggtaca aaggtcatgt caaactgttg ggcataggct
     3301 tcgatttcct gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaagattgc
     3361 ccttcaatct ggtaagtgtc ttccatgtaa agctcaaagg ttgagtagcc catgagagcc
     3421 aagacctcaa tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca
     3481 accatgtaag ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct
     3541 gctagaactg ttaccaacaa ggataaggca cgatagagtt ggtgaggttt gcggtaggtc
     3601 aattgatagt gaccttcctc accctggata gagatagatg cttggtcaga ctgagtgaca
     3661 gccacttcca catctggtag agaaatatga ccttttaaaa cctcaatagc ttgcgtttgt
     3721 ttgggactaa gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaagag
     3781 caccgtagag attggcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt
     3841 attcttcgta ggcatcgaca aagtcttcaa cagccttctt gacaccttgg ataaaatctg
     3901 gattttgact gatagagcca cccagactga tgacatctgg atcgatcaga tactggatat
     3961 tgagcaagcc ttgcgccaga ttgcggttca tgcgctcaat ggcttcttga caaaggatat
     4021 taccagctgc ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt
     4081 caatcacgta tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt
     4141 cagcaggggc aagggttgtc atgtagccaa attctccacc cagaccgtgg cgacctcgat
     4201 gaagtctacc attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg
     4261 cattttcaag ctctggatga gctagtagtt cactgagtcc aacgcagttg gcatcatttt
     4321 ctaaatggac aggtagctga taagagataa gcgcctcata ccaagaaaag ccatggatgt
     4381 agggcaccgc actgaagcca tcaatcacac ctgtctcttg attgactgca cctggaacac
     4441 tcatagcaat cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat
     4501 cctccaagtt ttcaggcgtt gaaatacttg tcttatccag tattttccca tcaggagtca
     4561 gactggcaaa cttaatccca gtccctccga tatcaatcgt tgcaatggtc attgttttta
     4621 ccataaaaag gggcgaatca gcagttagtt cttacttttt cgcccctcct ttcttttgat
     4681 gtttactttt tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa tctcttgtgg
     4741 agccaatagt cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa tggtttgttg
     4801 atgttctgat atgcgcacat tttcttgact catattatag taacggagga catagccttc
     4861 ttcattttcc gccactttaa aggctgttgg acaaacttgc ggtatgctga gaacagaatg
     4921 gctcaagagg ctaccagtcg caaccacgct tccttcctgt ctagcaagct gaaggctggt
     4981 aaacggtgtc tgcaaggctt tggcacgacg ataggctgag aagcgttctt gggcttggtg
     5041 gcattcaatc gcaaattcga cttcaaactc ccgcaagcat tgtgcttctg gcgttgggaa
     5101 gtagccccag tcacctagct cacctgatgc acgcaaaatg gtcacggcaa tagtgttatc
     5161 cccaaggatt tcgtattcat tcaatccctt attggataca gtcacccctt tttcatcgtc
     5221 atacagactg acaaaagctt gttggtgttg aggattttca ggattttccc atgaagcagc
     5281 tggtttgttt ggtcgtgtca ccacctcata gatactttca gaatcattac ttggacgcgt
     5341 gttatgagtc ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt
     5401 cttgaagcgg atttgtggat tgtcaacgaa gacagtcaac tcagtttcca gaggaatgtt
     5461 tgtcaattct tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc
     5521 ttcttctagc ttttcatccg cgctgacagg cacggtcaat tcatgtttga gcaagatttt
     5581 agcatagcaa gctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc
     5641 tgttcctttt ggttggaaat agatatactc gtttccgatg tccccacggt cttcaaagcg
     5701 gataaagtct tcataggctt cgtgagttgt cttatcatag actgtgatgt tgtcatccat
     5761 actcaccgtt acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc
     5821 ttgttctcct tccagcaatt ggaaggttgt ccaagaaagc ggtgctaggt gaactggaat
     5881 ggtcacgcgc acttgtcgag caatacgagc ttggcggaac ttgtcttttg gtaaatcgta
     5941 ttcaaaatta gctccgaggt cttcgattgt agcctctaca ggacgaccat ccaagtcctc
     6001 cacacggtaa cttggcaaga taagagcagc catctttttg tagccttctg ttgggtgcaa
     6061 ttccttgaaa tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg
     6121 caagcctgtg ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt
     6181 acccttccac tcgttgagca aattactttt aacaaagttt cctacttggt tgaccttggc
     6241 aaaacgcgtt tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg
     6301 tggcgcattc tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc
     6361 accagtgata atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa
     6421 ggcttgtttt aggtaaatgc gggatgaaga agtgttggca agtgtgtacc agccatctgt
     6481 ttcctgactg gtcaactcac ctgtaacagt tgataagtgt tcaggaagcg caccttctac
     6541 agcttgaaca tattcatcaa aggaactatg aacaaagatt acatccggga agagttcatt
     6601 tgccacacga atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat
     6661 catcaaccat tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa
     6721 ggtcaaggcc tcatctttgt caactggaat ttcattcccg ttactgtacc agttggcaaa
     6781 gagaatacct aaaacacgac taccatccac accctgccag tacatttctg aaaactggga
     6841 tgtaaactgc tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc
     6901 aaaggtcgcc acgtgaatgc ctgatttttg aagaatttga ggcgcttgtc ccatatttcc
     6961 aaaggtatct ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg
     7021 accaatcaag gtattgcgga cattggcttc actggagatc aaataatcat cctgcaagat
     7081 gtaaaaggga ccaattttaa gcttgccctc gtcaatgtag cgttggacct tgtcacgatt
     7141 ttcagggcga atttgtaagt agtcgtcaag gacaatagtt tgtccatcca agtggaaact
     7201 cttgaactca gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg
     7261 catacgatgg ctttcaaaag gcaagtacca ctcacggtcc cagtgactat gtgagataat
     7321 atgtacaaca atattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt
     7381 aaatgtaaac ttgtgattct gataagaatc ggttgagcta aagcgagccc cttagcgaat
     7441 atccaagtaa tctaagacca actcacagaa catcatgtta gcccaagaga accattcacg
     7501 agagtagagg gtcggatcat ctacgtggaa gctttcgtgc atgacacctg taccaccatc
     7561 gcaggcaacc agctgatcca gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc
     7621 ttggatagaa agggcgattg gccagatata gcgatagaag gtatgagaac ttcctagacc
     7681 gctagcgtat tctccttggt agaaatatgg attttcagag ctcaaaatgg tacgacgagt
     7741 agcttgatac acttcatcat cgaccgaaca gtagcccaga tagggcgcag ctagtagact
     7801 tggtacattt ggatcatcca tgatgctggc atttcctagg ccatccactt caaaagcgta
     7861 aatcttttcg cccttgctgt tggtggtgta agcgtagttt ttgattcctt cttggatttc
     7921 atcctgaaga cgcttggcat cagcaataac actctggcta tcagctaggt ttaatgctgc
     7981 gaagatttct tgcacataac ccaagactac tacagcaaac atatttgacg gaatcaagta
     8041 actatactga cagcagtcat cgctcggacg aaaggctgac caagtcatac ctgtcactgc
     8101 aaagtcaggt ccaaagccat catttaccaa ggtgtcttcc ttacggtccg tatctcggac
     8161 aaaacgataa ggagagttct tgtggtcttg ttccaccgtc cacagatgga gaatttcctt
     8221 agtcgctgcg acaaaagtct catcaaactg accagtctcg ccagtctctt tccagaggag
     8281 ataagccaac tgcaaaggat agcaaagcga atccacctca tacttgcgct cccagatcca
     8341 gccgttaagg tctgtgtggt cagtctcgtg gtgccctttc cagttctcct caatgttgaa
     8401 ggagttagca tagggatcct tgagtaccaa ggtcatctga cgtttgacca aacctgcaat
     8461 ggtctgacgc aggagggcat ctcttttagc tacatgaagg tagggtctga gttgggctgt
     8521 cgaatctcga agccacatgg caggaatatc cccagtcaag acaaaagttg aaccatcttc
     8581 taagatttca accgtattgt ccaaggtatc ggtgtagcaa cgctcgaaaa catccaccca
     8641 ctctggatgg tccttagccc gctctgctac ttcatctagc cattctctaa caatttcttt
     8701 cgaataaatc atcgtgcaat tccctctttc aaacaagttt catgttcagt atatagcttt
     8761 tgagacagaa aatacataca caaatgatag tcatttttct acaaaatagt tatctttgaa
     8821 actccttttc taaaggccct ctttttctga tataatgtag aaaaacagct aaaggaaaga
     8881 ctatgaaacc actacttgaa accatcgata cccgcttggg aactaccaac aagcatgcct
     8941 tttctcgggg aaataccctg ccatacacag gcgtgccttt tgggatgaat tactttgtgc
     9001 cccagaccag tgaccaggat ggatcttggt tcttcgatcc gcatctgcct atctttcagg
     9061 ggattcgatt aactcaccag cccagtcctt ggattgggga ctactcttgg ctccttctga
     9121 cacctgtcac cagtcaactg ggtggagaca gcctctttca ccgccaatct tcctatgata
     9181 gggataaggc ctctttccaa cctcattatc taaagatttt ctccctacgc tatcagattg
     9241 aaacccagct cacaccgact tgctatggtg cttctattcg tttgaagcaa aagcaaggca
     9301 aagccctctc cctctatctt cacgcagcag atgaactaac agttgagcag gtagataagc
     9361 ggactctggc tctcagacaa gaagggaaaa cagagaccaa taaaaatcca ctgatgctct
     9421 tcactgccct gcaaatgaac acggatattc ttgctatcag ccaagaagct ggagactggc
     9481 gaattgactt agcaagtagt caaaccgaga tgcaactagc cacttctttc atctctcctt
     9541 ctcaagctct aatcaatcta cctcaagaag attttgatag ctgtaaaaca agtgcccaag
     9601 cggattggga aaatctcctc catcgttttg acattataga gacaggagag gctgaccgaa
     9661 ccttctttga ccactgcctc tacagactct tcctcttccc acagactttt tatgaggttg
     9721 atgagtcagg gcaagccatc cacatggatc tggctactgg tactgtcaag cctggtgtcc
     9781 tctttagcaa caatggtttc tgggatacct tccgcaccac cttccccctc tttgccctta
     9841 tcataccgga gcactatcaa cgctttttag aaggtttcct caatagctac cgcgatactg
     9901 gtttccttcc aaaatggctg gctccagatg aacggggtat gatgccaggt acacttttag
     9961 acggtattat cgcagatagc gcctgcaagg acatggcccc tgacctagaa ggagaactct
    10021 tccaagccat gctcgaaaca gccagcaaag ccgaccctct cggaatcaat ggccgccacg
    10081 gactagccca ataccaagaa ctaggttacc tctctaccga ccaccacgaa agtgttagcc
    10141 atactctaga ctatgcctat agtgactttt gtatcgctag ctgtgccaaa aaactagaga
    10201 acatagaaat cgctgaaacc tacaaggctg cttcacaaaa ttaccgccag ctatttgacg
    10261 ctgagacagg ttacatgcga gcacgagaca atcaaggaaa ctttcaccct gacttctctc
    10321 cttatagttg ggggcgagac tacgctgaat gctctgccat tcaagctact ttaggcgttc
    10381 tccacgacat ccctggctta atccaactta tgggtggaaa agaaaccttt agcaactatc
    10441 ttttgaaagc ttgtcaagat gctcccctct ttgaaacaac aggctatggt tatgaaattc
    10501 acgaaatgag cgagatggct actgctcctt ttggacaaat agccatttcc aaccagccta
    10561 gtttccacat cccttatctc ttccgctaca gcgattaccc tgactacact gcccttctta
    10621 tcaagacgct ccgtcagaaa gcttttcagc caagttggga agcctatcct ggagatgaag
    10681 acaatggtag tctctctgct tggtacatct ggtcagctct cggattttat ccaacctgtc
    10741 caggaaaacc aagctatgac ctcggaatcc ctctctttga ccatctacga gtctacttag
    10801 ctaaagaaga taaatggctg gatatccata ctaaacaaaa ccacaaccat tttaactttg
    10861 tcaaagaatg ccgactggac aaaacactcg tatcaactat tcaacaccaa gacctcctaa
    10921 aagctgaaca actaactttc accctcagct ggctaccaaa tcactaataa ccaaaagaac
    10981 ctttgcatcg cgcaaaggtt cttcttttat gaaacttgga cttgaagttg atccacaagc
    11041 tgaccatcca ccgttaggtt cagatagaaa tccaaggttt tatctcctgc gaaatacaag
    11101 gttggtagcg tcagcctttt ttcagtctca ccagcttgaa acgcaaggac ttgaatctca
    11161 tcctgatagg cgacaccatg gacacctgtc cccggttgaa tcgaaatctt agcctctaaa
    11221 gaggagctac tttcactacg ttcaatccta aaatgaagag tctctccctt tgccacagct
    11281 aggctttttt ctgcaaatgc tagttcctga acaacttctt tttttgataa tccaggagtt
    11341 ttataaaggg aaatcttggt caatacagga atagattgtg cctcgataat catcacgcgc
    11401 accttttgtg cctctactag cggacctcgc aagaggcgct tatgaccaac tgtgaagccc
    11461 ctgccaaatt cctgccagac accatccacc tctacttgca catgaaaagc agcgattcgt
    11521 tgccctagct tcaaatcttc tcttaactca attacatcaa aagttttagg agaacctaaa
    11581 tcgagttcta actggatggg caagtctgca tcgcttgccc aagagctggt ctcaaggccg
    11641 tctgtcaaat ggcgacaagc aaagtctgcg gaaagagctg gaccagatac ctcagctccc
    11701 agagccaaat cttctttata gagctcattg cgataggtcg caaattcata aagtcgttca
    11761 atatcctttg catcaaagag cccagcttga ttcggcggaa tattaagcaa gagtggagtt
    11821 cctcgcccta ctgagtgaaa gtagatttcg accaactcct cgagagactt aggatcctga
    11881 tcctcatggt agaaccagcc tggacggatg gaaacatctg cctctccgat cgaaaaaatc
    11941 gtgcccgagg gatccccgtg ctgaagatag ttcagctctg cttctgttcc tagtttatca
    12001 ggattcatct tttgccacag tggatcacct gcatgccctc gttcattgcc aatccagtgg
    12061 atactggtgc cttctgttga aaaaatcaaa caatcgccct gcaagtcacg aatggtttca
    12121 aaccattttt caaattcata attaaccttt tgcgcgccct ctcctctggc accatccatc
    12181 caaacctcag cgaacttacc agcattccca tagttaggat ttgataagat ttccttcaac
    12241 tgagccagat aataggcatt gtagtccgct tctcggtcca catgatagag gggactatgg
    12301 gcatcccacg gtgacaggta gacccccata tccatatcaa actctgtggc agcttgggat
    12361 acttcaagga gcaagtcgcc ctctcctctc ctccaaggac tgaccttaac cgaataatct
    12421 gtgtgagctg tcggataaag gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt
    12481 ttgaagcccg tttccttgag cacacgaacc cactcacgcg catccaactg actcgggtta
    12541 aagcgctcag gatcctcctg tccagtcccc cattcttggt cataaaaggt attaggaccg
    12601 aagtggataa aagctgctag ttcatctccc agataagcta gctgagtctg acttggtaac
    12661 ggtccatgcg gtttgatttt cttcatcgtt atatatctcc ttctttagca acttctcttg
    12721 ttcacaaggt tatccacagg ttgtggataa taaaccatga tctaacagcc agctctttcc
    12781 aactccaact ggtaggctca ggatttctct cctcaatatt ccctcttcaa acagctctgt
    12841 catatggttc tgctaacacc tctcagaatg ctactctcca taattattct caggcttctt
    12901 tcaggtcatt gtgtgaagac cattcactct ccagactttt ccacagattg tgaataagtg
    12961 attattctaa actatcacct cgtcgactat aacaaaaaat aaaaagaggg tcaataagat
    13021 aattatctct tcctaaatac tcagaaaaga ttctataatt tgtacaattg aacaagaaat
    13081 gcttctccaa aatctatctt caaaagaatg ttgcatcttt ttatcctttc acacaaacat
    13141 ttctctttat acagatccaa gtgataacca gatgataatt ggacactttt tggtcatttg
    13201 tatatttttt gaaaaatttt ttaaaaaaat tttatatcct ttttaataga agtttatgca
    13261 tttgtatata tgtattacat cctatgttgt gtataataac ataaaattgt gctaaatatc
    13321 ataatttata ttattttccg agaaattata gccattgaca ttacctagga attgcgttat
    13381 aacatacatg taagcggtac ccaaaatggt gccaagtcaa aatttttaag gaggaaaata
    13441 catgtcttca catccaattc aggtcttctc agaaattggg aaactgaaaa aagttatgtt
    13501 acaccgtcca ggcaaggagt tagaaaactt gttgccggac tatcttgaaa ggcttctttt
    13561 tgatgatatt cctttcttgg aagatgctca aaaagaacat gatgcatttg cccaagctct
    13621 tcgcgatgaa ggaattgagg ttctctacct agaacaactc gctgctgaat cattgacctc
    13681 tccagaaatc cgcgatcaat ttatcgagga atacttagac gaagccaaca tccgtgatcg
    13741 tcaaaccaag gttgctattc gtgaattact tcacggcatc aaggacaacc aagaattggt
    13801 tgaaaaaaca atggctggga ttcaaaaagt tgaattgcca gaaattcctg acgaagctaa
    13861 agatctaact gacttagttg aatccgatta tccatttgca attgacccga tgccaaacct
    13921 ctatttcact cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta accacatgtt
    13981 tgcagacact cgtaaccgtg aaacactcta cggtaagtat atcttcaaat accacccaat
    14041 ctatggcgga aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta tcgaaggtgg
    14101 agacgagcta gttctttcta aagacgtcct tgcagtaggt atctctcaac gtacagacgc
    14161 agcttctatc gaaaaacttt tggtcaacat cttcaagaaa aatgttggct tcaagaaagt
    14221 tttggccttt gaatttgcta acaaccgtaa attcatgcac ttggatactg tcttcactat
    14281 ggtagactat gacaagttca ctattcaccc agaaatcgaa ggcgaccttc acgtttactc
    14341 agttacttac gaaaacgaaa aacttaaaat cgttgaagag aaaggtgact tagctgaact
    14401 tcttgctcaa aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg gtggcaatat
    14461 cgtagcagct gcgcgtgaac aatggaacga cggttctaac actttgacca tcgcacctgg
    14521 tgtggtagtt gtttatgacc gcaataccgt gaccaataag attttggaag aatacgggct
    14581 tcgcttgatt aagattcgcg gaagtgaatt ggttcggggc cgtggtggac ctcgttgtat
    14641 gtctatgcca tttgaacgtg aagaagtgta atcgctgttc ggtattcgtc aatagaaaat
    14701 gtaaaaaata gaaagaggaa ataataaaat gacaaattca gtattccaag gacgcagctt
    14761 cttagcagaa aaagacttta cccgtgcaga gttagaatac cttattggtc tttcagctca
    14821 cttgaaagat ttgaaaaaac gcaatattca acaccactac cttgctggca agaatatcgc
    14881 tctcctattt gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg cggctatcga
    14941 ccttggtgct cacccagaat acctcggagc aaatgatatt cagttgggta aaaaagaatc
    15001 tactgaagat actgctaaag tattgggacg tatgtttgac gggattgaat tccgcggatt
    15061 cagccaacgt atggttgaag aattggcaga attctcaggc gttccagtat ggaacggtct
    15121 aactgacgaa tggcacccaa ctcaaatgct cgctgactac ttgactgttc aagaaaactt
    15181 cggtcgcttg gaaggcttga cattggtata ctgtggtgat ggacgtaaca acgttgccaa
    15241 cagcttgctc gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct caccaaaaga
    15301 actcttccca gaaaaagaaa tcgttgaatt ggcagaagga tttgctaaag aaagtggcgc
    15361 acatgttctc atcactgaag atgctgatga agcagttaaa gatgcagacg ttctttacac
    15421 agacgtttgg gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag ctcttcttaa
    15481 accttaccaa gtcaatatgg acttagttaa aaaagcaggc aatgaaaact tgatcttcct
    15541 acactgcttg ccagcattcc acgatactca cactgtttat ggtaaagacg ttgctgaaaa
    15601 atttggtgta gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt acgctcgcca
    15661 cttcgatcaa gcagaaaacc gtatgcacac tatcaaagct gttatggctg ctacacttgg
    15721 taacctttat attcctaaag tataatttta gataataaac cgtctaccaa cagctatggg
    15781 ggctgcgact aatagcttta gtccggtcct cttttatata atggtaatct attatttctt
    15841 ataaaatatg tgaaaaatca ttaaattgaa atctaaacgc attctattga gtgtgataaa
    15901 ggagaattta tggcaaatcg taaaattgta gtagctttgg gaggaaatgc gattctttct
    15961 tctgacccat cagcaaaagc tcaacaagaa gctttagttg aaacagctaa gcatcttgta
    16021 aaattgatta aaaatggaga tgatttgatt atcactcacg gtaatggacc tcaagttggg
    16081 aatctcttgc tccaacattt ggcatcagac tctgaaaaga accctgcctt cccactcgac
    16141 tcacttgtcg ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc tttgcaaaat
    16201 gctctcttag atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca agttgtcgta
    16261 gataaaaatg atccagcttt tgttaacttg agtaaaccaa tcggtccttt ctattcagaa
    16321 gaagaagcaa aagcagaagc cgaaaagagc ggagcgactt tcaaggaaga tgctggccgt
    16381 ggctggcgta aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat tgaaaccatc
    16441 cgtactcttt taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg tattcccgtc
    16501 gtcaaagaaa acaatggaca tttgactggt gtcgaagcgg ttattgataa agacttcgct
    16561 tcccaacgtt tggcagaatt ggttgaggca gacctcttca tcgttttgac aggtgtagat
    16621 tatgtatttg ttaactacaa caagccaaac caggaaaaat tggaacatgt gaatgttgcc
    16681 cagctggaag aatatatcaa acaagatcag tttgcaccag gtagcatgct tccaaaagta
    16741 gaagcagcta tcgcttttgt caatggtcgt ccagaaggaa aagcagttat tacttccctt
    16801 gaaaatctag gcgccttgat tgaatctgaa agcggaacaa ttattgaaaa aggataagtt
    16861 gttttactaa taagatgtat tctatttcta gtatctttat atcaaattag aaattattct
    16921 tgaaaacatg tacaatattt caaaagatac tagtttttta gactttaata tggtaaaaca
    16981 aatataaata gaaagcgttt tcttaaatgt ttatttaaga aagtagttgg ttttttacac
    17041 tttgttagac atcaggagga aaaacaaatg agtgaaaaag ctaaaaaagg gtttaagatg
    17101 ccttcatctt acaccgtatt attgataatc attgctatta tggcagtact aacttggttt
    17161 atccctgcgg gggcctttat agaaggtatt tacgagactc agcctcaaaa tccacaaggg
    17221 atttgggatg tcctcatggc accgattcgg gctatgctag gtactcatcc agaggaaggt
    17281 tcgctcatta aagaaacgag cgcagcgatt gatgtagcct tcttcatcct tatggttggt
    17341 ggtttccttg gcattgtcaa caaaactggt gctcttgacg tagggattgc ctctatcgtg
    17401 aagaagtata agggccgcga aaaaatgtta attttggtac tgatgccttt gtttgccctc
    17461 ggtggtacaa cttatggtat gggggaagaa acaatggcct tctatccact ccttgtgcca
    17521 gttatgatgg ccgttggttt tgatagcctg actggtgttg caattatttt gctcggttct
    17581 caaatcggct gtttggcatc tactctgaat ccatttgcga caggtattgc ttcagcgact
    17641 gcgggagttg gtacagggga cggtatcgta cttcgtctga tcttctgggt taccctgact
    17701 gctcttagta cttggtttgt ttaccgttat gcggataaga ttcaaaaaga tccgactaag
    17761 tcactggttt atagtactcg caaagaagat ttgaaacact ttaacgtaga agaatcttca
    17821 tctgtagaat ctacacttag cagcaaacaa aaatcagttc tcttcttatt tgtgttgaca
    17881 ttcatcttga tggtattgag cttcattcca tggacagacc ttggcgttac catttttgat
    17941 gactttaatg cttggttgac tggtcttcca gttattggta atattgtcgg ttcatctact
    18001 tctgcactag gtacttggta cttcccagaa ggcgcaatgc tctttgcctt tatgggtatc
    18061 ctgattggta ttatttatgg tcttaaagaa gataagatta tctcttcctt catgaatggt
    18121 gctgctgact tgctcagtgt tgccttgatc gtagcgattg ctcgtggtat tcaagttatc
    18181 atgaacgacg gtatgattac cgatacaatc ctcaactggg gtaaagaagg cttgagcggt
    18241 ctatcttcac aagtctttat cgttgtaact tatatcttct atctacctat gtcattcttg
    18301 atcccatctt catctggtct tgccagcgca actatgggta tcatggctcc acttggagaa
    18361 tttgtaaatg tccgtcctag cttgattatc actgcttacc aatctgcttc aggtgtcttg
    18421 aacttgattg caccaacatc tggtattgtg atgggagctc ttgcacttgg acgtatcaac
    18481 attggtactt ggtggaaatt catgggcaaa ctcgtagtcg ctattattgt agtgaccatc
    18541 gcccttcttc tccttggaac cttccttcca ttcctataaa atagtgagtg aggtgatttc
    18601 atgaaaatag atataacaaa tcaagttaaa gatgaatttc ttatatcatt aaaaaccttg
    18661 atttcctatc cttcagtact caatgaagga gaaaatggaa caccttttgg acaagcaatc
    18721 caagatgtcc tagaaaaaac tttagagatt tgtcaagaca taggtttcac tacctatctt
    18781 gaccctaaag gttattacgg atatgcagaa atcggtcagg gagcagagct tctggccatt
    18841 ctctgtcatt tggatgttgt tccatcaggt gatgaagcag attggcagac accgccattt
    18901 gaagcaacta tcaaagacgg ctgggtattc ggacgtggtg tccaagatga taaaggccct
    18961 tcgctcgcag ctctctatgc agtaaaaagc ttgctggacc aaggtattca gttcaaaaag
    19021 cgcgtacgct ttatctttgg taccgatgag gaaaccctct ggcgctgcat ggcacgctac
    19081 aataccatcg aagaacaggc cagcatgggc tttgcacctg actcatcttt tcctctgacc
    19141 tatgctgaaa aagggcttct acaggtcaaa cttcatggcc ctggatcgga tcaactagag
    19201 cttgaagtag gaggcgcctt taacgttgta ccagacaagg ccaactacca aggtcccctc
    19261 tatgaacagg tttgtaacgg tctcaaagaa gctggttatg attaccaatc cactgaacaa
    19321 accgtaacgg ttctcggagt gccaaagcat gctaaggatg ctagtcaagg tatcaatgct
    19381 gtcatccgac tagctaccat tcttgctcct ctccaagaac accctgctct cagttttctt
    19441 gcaacacaag caggtcaaga cggcacagga agacaaatct ttggtgatat agcagatgaa
    19501 ccttctggtc acctatcctt taatgtcgca ggtctcatga tcaatcatga acgttctgaa
    19561 atccgtattg acattcggac tcctgtctta gctgataagg aagaactagt agagttgctt
    19621 acaagatgtg cacaaaacta ccaactccgc tacgaagagt ttgactatct agcgcctcta
    19681 tacgtcgcaa aagacagtaa actcgttagc acactgatgc aaatctacca agaaaagact
    19741 ggcgataaca gtcctgctat ttcatccggt ggtgccactt ttgctcgcac catgccaaat
    19801 tgtgtagcct tcggcgcctt attcccagga gcgaagcaga cagaacatca ggcaaatgaa
    19861 tgtgccgttc tagaagattt gtaccgtgct atggatattt atgccgaagc cgtctatcga
    19921 cttgcaactt aatcaggcaa ctgtttctac caaaaaaaat cgaccgatta atgaactgca
    19981 ccccaaaagt tagacagaat aaatctaact tttggggtgt tttattatga aattgagtta
    20041 tgaagataaa gttcagatct atgaactaag aaagcaagga caaagcttca aacagctttc
    20101 aaaaagattt gtgtggatgt ttctggtcta aagtcatctg aatctttgag atgagcttta
    20161 taaatcgatt ttttcagttt ttgcactggt gtttcgataa actcaaactt tttagccgtg
    20221 gtattgcctg attttatagt atattgaaac tagaatagta cacctctact tctaaaacat
    20281 ttttagaaat cgatttgact gtcctgaacg atttgtcctg ttcttatttc attttacgat
    20341 atttgagcca cttcgtcttt aacggcttta ttcataagct cttgtaattt ttctttacta
    20401 tcaattactt ctgattttcc gttgtaattt attgtaatag gttttaactt acctaatttc
    20461 tcgacacgct cattaatttg atcttttttg aaggctgcat atgtttttcc taagattttt
    20521 tcgaaaatat atttatcaga tagcggtttg tcttcttctt cagcttggtt tttgtattga
    20581 tttgaaacgt aaggaacaaa tccttcatag taacctaata ctcccataag ttcaaaagct
    20641 tgttttctaa ttcaaaccat tgcaactcag atttcagctt ttcagataaa tcctgctcat
    20701 ccaaataatg acttgaaatt agtgctgaac tcgtttctgt atcctgtaca ggctgagcac
    20761 ccataccagc aaaaaataaa ctcgttccta gcaagaccga acaagctcct attgcatatt
    20821 tcctcaaaga aaaacgctgc tttctctcaa attgaaattc tttcatccca tctcccatca
    20881 ttcattatta ctgtatattt tgtatatcag aaatagtttg tattcacaaa tctttctagt
    20941 tattccctta tcattcctaa ttaagggaga taacatacaa taatttttag ttaaatgtat
    21001 atcgatgttt tttgtttttc ttaataaacg caatacaaaa agagcctgtt gccaagctct
    21061 ttgtactcaa tgaaaatcaa agagcaaact aggaaactag ccacaggttg ctcaaaacac
    21121 cgttttgagg tagtagataa gactgacgaa gtcagctcaa aacactgttt tgaggttgca
    21181 gatagaactg acgaagtcag ctcaaatata cggcaaagcg acgctgacgt ggtttgaaga
    21241 gattttcgaa gagtattagt ctattatttc ttctcagcgc gaagggctga caagatttgt
    21301 gttcggatat catccacacc atttggagta tttggtaaaa agatagtttg atttccttta
    21361 gaggcaaagg tattcaaggt atccaaatac tggttggtca agaggataga catgatttgt
    21421 tcttctgtca tgccaacatt ggcttccttg agttcggtga tagactctgc caatccatcc
    21481 acaatcgcct tacgttgttg ggcaatcccc acaccatgaa ggcggtcttt ttctgcttct
    21541 gcttcagctg cagtgacaat tttaatcttg tcagcttccg ccaattcttg tgctgcgacc
    21601 cgcttacgtt gcgccgcatt gatttcattc atggattgct taacttctgc atctggttcg
    21661 accttggtaa tcaaggtttt cacgataatg tagccgtaag tggtcatttc ttctgctact
    21721 tggtgttgaa cctcaagggc aatctcatct tttttctcaa acaattcatc caaggttaat
    21781 tttggaacag aagaacgaag agcatcttcg atataagatt taatctgaga ttctggacgt
    21841 atgagtttat agtaagcatc tgtcacgctc tgctcgttga cacggtactg agtcgctaca
    21901 ttcatcataa cgaacacatt gtccttggtc ttagtctcaa ccacaatatc actttgcaac
    21961 aagcgcaact gaatccgtgc tgcaatcgag tcaatcccaa aaggcaagcg aatatgaata
    22021 ccgctattgg caaccttttg gtatttccca aagcgttcaa taatcgccac cgactgctga
    22081 cgaaccacat aaactgtact cagtgtgact atcaccaata ggagcacaca aacaatcaga
    22141 aaaatcatga aaaatattgc cataatggaa cctccacaag tatttttcta gtattatagc
    22201 acatttaaag aaggctgtgc cgtttttact gcgatttttc ctgaaatgtc aataattaga
    22261 ggtgaattgt cctattgtcg tccaatctct tgctaaaata actctttata aaaggcaatc
    22321 gtttcttcta aggttggcat aaatggattt cctggtgcgc aggcatcaat caaggcattc
    22381 ttagaaaggt attcaaagtc gaaatctttt tcttcaatac caagttcagt cagtttctta
    22441 ggaataccta ctgtctcaga aagcttctca atctcaacaa tcgcataatc ggcacattct
    22501 tgatctgatt taccttctac atgaagtccc aaggctttgg caacattgcg gaaagcttct
    22561 ggtacacgtt tagcattttc acgttctata actggtagca acatggcaca gcaaacacca
    22621 tgtggcaaat tatatactgc accgagttgg tgagccattg agtgaacata accaagacca
    22681 gcattattaa agctcatacc accaaggaag atggcattga ccataccttc acgcgcttca
    22741 atatcatgtc cattttctac agcacgagga agatattcct tgataagctc aatagcacca
    22801 atagacagtt tcttggttac atcataagca ccaggtgtta ccaaagcttc aacagcgtga
    22861 gtcagagcat ccataccagt agcagcagtc aggcctttag gtttggaaag catgagttcc
    22921 gggtcattaa cagagataag agcaaggcta ttcttgtcaa ccattaccat cttaaccttg
    22981 cgttcttcat cagtaatcac atagttaatg gtaatttctg ctgatgtacc tgccgttgta
    23041 ttgatagcca caactggcaa gcctttttta gcagacttat gaagcccttc ataatcctgt
    23101 ggttttccac catttgtagc catgatagaa atacaactag ctgcatcctg tggagaaccc
    23161 ccgccaagac tgatgataaa gtcacagcca tgttcttgca aagcagctac cccatctgtg
    23221 acatttttac aagtagggtt tggctctaca tcgctaaaga tgacatattc gattccttct
    23281 gtatccagtg gttttaaagt cttaggtaaa atatcactgc cttcgatgta cttatctgtc
    23341 accaaaagtg cctttttata gccaagttcc ttgatataag gccccacttc atttacaaca
    23401 ccttttccaa taaggttgac tgccggaaca taaaatatag ccatataatt ttcctcctgc
    23461 gcctccgcgc tatctattta acatcattat attacttttt gtattcgttt tcaattattt
    23521 gtgaattgtt taacaaatct ttttaaaagg ataggaggga cgtaagttca cctcctatat
    23581 aaacaagttt tatttatgta gtggtcccaa caactgacat gcacgatagt ctgctgattc
    23641 tagatcttct gttccaaata aggaccaatt tttaggtcta aagatatctt cctcaggtac
    23701 attatgcata ttgacaggaa ttctcaacat agaagccaag gtaatcaagt ctgctccaat
    23761 gtgtccatat gttatggctc cgtgattagc tccccaatta ttcatgacgt catagacaga
    23821 cttgaaagca ccttttcctg tcaaacgtgg agcaaaccaa gtagttggcc atcctggatc
    23881 tgtacgatta tctaaagtat ggtgaacatc ttcaggaagt tcaagtgtgt aaccttctgc
    23941 aatttgtagc actggaccaa ccccttttag aagattgaga cgtaccattg ttactggcat
    24001 atcccccttc gtcaagaaac gagttgagaa tcctcctcca cggaagtatt cgcggtttgc
    24061 tggtgggaag tctgtatttt caagcatagc ctgcacttca ctttcttcca actcccagaa
    24121 tggtttcata acaggtttgc catctagagt agcttgacct gtaccatcca atgtacaaga
    24181 tccagagttg attagatgta agaagccagc tgcagcacga ccctctaaag tatgtcccgt
    24241 tacacgttta acagcctctg ggctccaata agtacgcaca tcagcaaaga tttgtggagt
    24301 atttgttaat agataattaa agagcataga cacaccattt agtgaatcat tctctgtcgc
    24361 aaatacaaat ggttttcgaa taccattcca gtcaaactga gtattgagga aagtttccat
    24421 aaaatctcca tttggaaaat ggtctgtcca ctgacgttga ccttggaaac cagctactaa
    24481 agcatggtga ccaactgctt cttcttcaaa accaagttca gcaagtcttg ggttaccaac
    24541 cattaagtca cgtccaatca tgaacatctt aataacaaat tcccattgtt tttctttctc
    24601 ttctttagaa agaatcaaat cctcacgatt tcggtcaact ccttctttga tatgttcttt
    24661 tacccaaacc atagcacgtt caaactcttc agggtcgtaa ataccacggt ccatacggcg
    24721 tgtgaactcc gtcatatcta ccgattcatt tcgcattcct aagtattctt ggaagaaatc
    24781 cggatttaca atagaaccac caatccccat cgaaacacta cccattgata ggtaagcagt
    24841 gtctctcatc aagccagttg caagagctgc acgcgcataa cgtaaaagtt tttctttgac
    24901 atcttctgga atatctgtat cattagcttc ctgaacatct ctaccataaa tcccaaaggc
    24961 tggaatccct ttttgagcat gtgaagctag tacagctgca agatagacag ctcctgggcg
    25021 ttctgtccca ttaaatcccc aaatagcatg aggaatatct ggagacatat ccatagtttc
    25081 actaccataa caccagcatg gtgtaactgt aattgttgcg caaacatttg attttttaaa
    25141 caactcatgg gaagctgcag cctctggaac acgaccaatg gtagatggag aaatcacaca
    25201 ttccacaggt tccccatctg gatatttcaa tgtgcttgaa atcaaatctg ccacactttt
    25261 agccatgttc attgtttgta cttcaagtga ttcgcgtaca ccttgacgac gaccatcaat
    25321 agtcggacga ataccaatac gtggatgttg aatcatacta ttttcctctt atttttctgt
    25381 ttctttcata ctaccatcgc cattatattt acggtagcct gggtgacgac ctgtaacctt
    25441 ataattttct cgcattgaga atagacgttc taaagtcgga cgagcaattt cttgctcctc
    25501 aatgcccttt gtagaaagta acattcttcc gtggaaggtt gtctttgcga ctaattctaa
    25561 agtttccata cggtagtatg ctgtaatgac atcgcttccg acagtcagag ctccatgatt
    25621 ttctaatagc atgacatcat gatcgggcag ataaggtgta attgcttctg gcacttccat
    25681 tgtagacgga actccaaatg gcgttatagg aattgcccct accacaatcg cgctctcaat
    25741 tagtgaataa gtatctaaag gaatgtgtgc aagagcaaat cctgttgcaa tcggtggatg
    25801 cgcgtgaaca actgaacgaa catcctcacg ttcttcgtag caccgaatgt gcattttaat
    25861 ttcactagaa ggacagtaat caccttctgc ttctaaaatc tctcctttaa gatttaactt
    25921 caccagcttt tctggtgtaa taaaactttt gctgatacca gtaggtgttg caagaatggt
    25981 atcctcatct aatcgaacag atacattccc atcgttagca gcaacccaac caagttgcca
    26041 catcttatga caaacatcac aaatctgttc acgaattaat ttttcatctt gagacataaa
    26101 ctcactcctt tctaaaaatg aagttcgaat tcaagatttc cgttactgtt aatggtaata
    26161 gtaaccatat gatttttctg atcaaaattg agagatggct gatcatagga attatttgtt
    26221 cctgataatg aatgaatagt aggaagttta tctatacctc ttaatactat ctttgtaacc
    26281 cttttctctc caaactgagt atctttgata taatgttctt caatccactt aatcgcacct
    26341 ttcttggtca attgtggtag tttctttgcc tgatttgcat cttgagcatt agaccatagt
    26401 gaatctttat ttgttctaaa attatttaaa ttcactttca atccattact tattctctca
    26461 aaaataccat aggtatgagg ttcaaattca acatccat
//
LOCUS       DAVMHG010000012        26218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMHG010000012 DAVMHG010000000
VERSION     DAVMHG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.012"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(256..327)
                     /locus_tag="VYR08_000410"
     tRNA            complement(256..327)
                     /locus_tag="VYR08_000410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Arg,seq:cct)
     gene            complement(370..717)
                     /gene="rplS"
                     /locus_tag="VYR08_000411"
     CDS             complement(370..717)
                     /gene="rplS"
                     /locus_tag="VYR08_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV3465991.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(836..1165)
                     /gene="crcB"
                     /locus_tag="VYR08_000412"
     CDS             complement(836..1165)
                     /gene="crcB"
                     /locus_tag="VYR08_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3465992.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1159..1533)
                     /gene="crcB"
                     /locus_tag="VYR08_000413"
     CDS             complement(1159..1533)
                     /gene="crcB"
                     /locus_tag="VYR08_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV3465993.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1530..1796)
                     /locus_tag="VYR08_000414"
     CDS             complement(1530..1796)
                     /locus_tag="VYR08_000414"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV3465994.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1912..2355)
                     /locus_tag="VYR08_000415"
     CDS             complement(1912..2355)
                     /locus_tag="VYR08_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV3465995.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2459..3394
                     /locus_tag="VYR08_000416"
     CDS             2459..3394
                     /locus_tag="VYR08_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV3465996.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3448..3732
                     /locus_tag="VYR08_000417"
     CDS             3448..3732
                     /locus_tag="VYR08_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV3465997.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3789..4007)
                     /locus_tag="VYR08_000418"
     CDS             complement(3789..4007)
                     /locus_tag="VYR08_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3465998.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4004..4755)
                     /locus_tag="VYR08_000419"
                     /pseudo
     CDS             complement(4004..4755)
                     /locus_tag="VYR08_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5291..7018)
                     /gene="ezrA"
                     /locus_tag="VYR08_000420"
     CDS             complement(5291..7018)
                     /gene="ezrA"
                     /locus_tag="VYR08_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV3465999.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(7100..9046)
                     /gene="gyrB"
                     /locus_tag="VYR08_000421"
     CDS             complement(7100..9046)
                     /gene="gyrB"
                     /locus_tag="VYR08_000421"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV3466000.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(9061..9624)
                     /locus_tag="VYR08_000422"
     CDS             complement(9061..9624)
                     /locus_tag="VYR08_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3466001.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(9709..10263)
                     /locus_tag="VYR08_000423"
     CDS             complement(9709..10263)
                     /locus_tag="VYR08_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV3466002.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(10274..11497)
                     /locus_tag="VYR08_000424"
     CDS             complement(10274..11497)
                     /locus_tag="VYR08_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3466003.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(11534..13984)
                     /locus_tag="VYR08_000425"
     CDS             complement(11534..13984)
                     /locus_tag="VYR08_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV3466004.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(14184..15113)
                     /locus_tag="VYR08_000426"
     CDS             complement(14184..15113)
                     /locus_tag="VYR08_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3466005.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVHKLHPKNRFLDTGFYDYDPDMVR"
     gene            15253..15540
                     /locus_tag="VYR08_000427"
     CDS             15253..15540
                     /locus_tag="VYR08_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV3466006.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(15574..16908)
                     /gene="ciaH"
                     /locus_tag="VYR08_000428"
     CDS             complement(15574..16908)
                     /gene="ciaH"
                     /locus_tag="VYR08_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV3466007.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(16898..17572)
                     /gene="ciaR"
                     /locus_tag="VYR08_000429"
     CDS             complement(16898..17572)
                     /gene="ciaR"
                     /locus_tag="VYR08_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV3466008.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(17681..20227)
                     /locus_tag="VYR08_000430"
     CDS             complement(17681..20227)
                     /locus_tag="VYR08_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV3466009.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(20350..20790)
                     /locus_tag="VYR08_000431"
     CDS             complement(20350..20790)
                     /locus_tag="VYR08_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV3466010.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCVVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(20765..21634)
                     /locus_tag="VYR08_000432"
     CDS             complement(20765..21634)
                     /locus_tag="VYR08_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV3466011.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAESHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGVKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(21676..22404)
                     /locus_tag="VYR08_000433"
     CDS             complement(21676..22404)
                     /locus_tag="VYR08_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466012.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(22412..22891)
                     /locus_tag="VYR08_000434"
     CDS             complement(22412..22891)
                     /locus_tag="VYR08_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3466013.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(22902..23600)
                     /locus_tag="VYR08_000435"
     CDS             complement(22902..23600)
                     /locus_tag="VYR08_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV3466014.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(23593..23829)
                     /locus_tag="VYR08_000436"
     CDS             complement(23593..23829)
                     /locus_tag="VYR08_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV3466015.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(23929..24855)
                     /locus_tag="VYR08_000437"
     CDS             complement(23929..24855)
                     /locus_tag="VYR08_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV3466016.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(24930..25745)
                     /locus_tag="VYR08_000438"
     CDS             complement(24930..25745)
                     /locus_tag="VYR08_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466017.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(25749..26069)
                     /locus_tag="VYR08_000439"
     CDS             complement(25749..26069)
                     /locus_tag="VYR08_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3466018.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         7973 a         5711 c         4609 g         7925 t
ORIGIN      
        1 gttccactgc tgaagaaata aacaattact taaagaaata tgaatgttat agtcctttaa
       61 ataatttaga acttgacgat tttgaataaa ttgactatac aaacatttat atactcgata
      121 tagtctcaat tttatctgat gattgcccaa atttttcaat aataaaacgc ataatattat
      181 ggagacaatc ccctatatta tgcgttcttt taatatcaaa gactttttga caaacttctt
      241 tgatatctaa ttacatgccc cctgcaggaa tcgaacctgc aactactcct taggagggag
      301 ttgttatatc cattgaacta agggagctag ataaaaactc tgctgaaaaa gcagagtttt
      361 ttagtcgaat taacgacgga tttctttgat acgagctgcc ttaccttgaa gagcacgcaa
      421 gtagtacaat ttcgcacgac gtactttacc gtaacgtaca acttcgattt tttcaacacg
      481 tggagtgtgg attgggaaga tacgctcaac acctacaccg ttagagattt tacgaactgt
      541 gtagttttca gagatgccag cacctttacg tgcaataaca acaccttcaa aaatctggat
      601 acgttcacgg ttaccttcga caactttcgc atgtacacga acagtgtcac caggacggaa
      661 tgatgggata tctgtacgaa gttgaccttc agtcaagctt tggattaatg gattcatttt
      721 attctcctat ctttgtcaat cttgaggaat cttcctcagc ggataaactg tatttttgtg
      781 cgtccattac acacaagata tagtttacca aatttctaaa aaaaagtaaa gaaatttata
      841 gcaaaattcc taaaaaaatc gcaaccaaac cacctatgta agtcaaagtc aggtaagaat
      901 aaaagacctt cttatcactt agcagtcttt gaagttcgtc attcaaggtc gaaaaagttg
      961 ttaaaccgcc acaaaatcct gttgctagaa tagcatatac ttccttagac tctacatgat
     1021 tgtagaatac tccaattaaa aaacaaccta aaagattagc tatgagcgtt ccgagtggca
     1081 atttagaagc ttgattatag cgagaaaaga aataccgtac gagtgctccc aagccacagg
     1141 cgattgcaag atagacgatt accatttctt cctccccaaa tagtaagcta aaagcaggcc
     1201 tccaccgata ctcaaaagca aatacaggac taaactaaaa taacgccctg tatcaagcag
     1261 cttcacagta tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc
     1321 cagtgctaaa atcaagccct tactagtccc cttatagacc agatagccct tgacaagaaa
     1381 aatcaagcag aaaattccca gatagttgac aaagagagtg ccccaaggaa agtctggact
     1441 ggctggtaac caggtggaaa ctagatatcg gacaagtccg cccaacatag cagctagaaa
     1501 aattcctagc ggataaaatt gttctttttt catttgatat tttgatcctg ataatcacgc
     1561 gaacgtttga gtatgtccga aaaagtcgcg acaatagtct cctgatagcg cttgtcctct
     1621 acacgacttc taaccttttc aaaaataact gcttctctct tagtatctaa aatcggtttc
     1681 cctgaagcct tcttataagc gacaactcct tcaactaagt gcattcgttc ttctaacagt
     1741 ttgacaattt ggtcgtcgat ttgatcgatt tcttgacgaa taatatctaa atccatacag
     1801 tctcctcctt tatttgaatt attgtatcaa aaagccatca attaggctag taaaatccca
     1861 actctcgata agaaaaatgc actgattgat tacatcagcg cacttttatg cttatcctac
     1921 tttagcagcc aattcttctg cgaattgttc caagcgttca atatcttctt cctcggcaga
     1981 aagatcaact ttaacacact ctgaaccttt ttctgctcct gttgacacaa agacacggtc
     2041 aaagtcatca acagccttac aaaattcatc gtagaaggta tctcctgagc cgaccactcc
     2101 gtagattttg ccattcaagt tgagatctgc taggtcttcg tagaagtcca tcatctcatc
     2161 tggcaattca ccatcaccat aagtataggt cgcaacgata gcgatgtctg cttccaagaa
     2221 gtctgaagcg tcaacagttg tacattcatc aacatcaaca tccaagccca agtcacgtaa
     2281 tttgtctgct acaatatctg caatttcttc ggtattaccg gtcatactgg caaatacaat
     2341 ttttgctaat gccatatcgt cctcctcaat ttatctttct ccattatatc acatcttttt
     2401 cattttaaaa ataaaaattc ttgtgctaca atgaaatgag aaagaattga ggttatttat
     2461 ggagatttgc caacaaattt tagaaaaaat taaagaatac gacaccatta tcattcatcg
     2521 tcatatgaaa ccagaccctg atgccttggg aagtcaggtg ggattgaaag ccttgctaga
     2581 acatcatttc ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg
     2641 gatggctgag atggatcttg ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg
     2701 tgatacagct aatactgctc gtatcgatga taagcgctat agtcaaggtg attttctcat
     2761 taagattgac caccatccaa atgatgatgt atacggtgac ctgtcttggg tcgatactag
     2821 ttcaagtagc gctagcgaga tgattaccct atttgcccaa acaacccaac tagccttggc
     2881 agatcgcgat gctgagttgc tctttgcagg aattgtcggt gatacaggtc gcttcctcta
     2941 cccttctacc actgcacgga ctcttcgcct ggctgcttat ttgagagaac ataactttga
     3001 ctttgcggct ctcactcgca aaatggacac tatgagctac aaaattgcta aactgcaagg
     3061 ctacatctac gaccatctgg aagtggatga aaatggtgct gctcgcgtta tcctgagtca
     3121 gaaaatcttg aaacaataca atataaccga tgctgaaact gcggccattg taggtgcacc
     3181 tggacgcatt gacagagtga gtctctgggg aatttttgtc gaacaggctg atggccacta
     3241 ccgagttcgc ttacgcagta aagtccatcc tatcaatgaa attgccaagg agcatgatgg
     3301 tggaggccac cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa acgaaatcat
     3361 ctaccaaaag ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa
     3421 tctgatagac tagtatagta acaatctatg gctcgcaaag agaccatggc agaaaggaaa
     3481 tattgcaaaa tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt catggacaca
     3541 actactggtt accaattcct tagcggttca acaaaacgct ctaacgaaac agttgagttc
     3601 gaaggcgaaa cttacccatt gatccgtgtg gaaatttcat cagactcaca cccattctac
     3661 actggacgtc aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac
     3721 ggtctcaaat aatgataaga gaacagtttt ggctgttctt ttttgtttct tgaaatcaac
     3781 tgctgttttc atgttccaga ctcatctgta ggttcgattt ccatgctact aggcaggaag
     3841 gaaatagctg tttcaacacg tccataatga gctatactat tgtcacgaac cacactttca
     3901 ttgatggtcc aagtggaatt cattttctta aaagcttctc ggactttttc caaatctttg
     3961 gaggcaatgg cctgctctaa ggtttcaaaa cgaggactta tactcatctg ctttcaaaaa
     4021 gcattctagt ccatctccga ttaccgatgg actttatcac ctccttctcc agtccttgta
     4081 tgacatcttg aagttgattc atgacatctt ccaaagttcg aaaggcttta ttcttaaatc
     4141 cacgtttacg aatctctttc cacacttgtt caatgggttc atctctggtg tgtatggagg
     4201 aataaaggta aaatcaatat tagtcggaat atttaaggta cttgatttat gccatatagc
     4261 attgtccata acgagtaaaa ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc
     4321 acactccttt ttataaacct gaaataaggc atcaattgta acaaattctc ctgcctctgt
     4381 agccttcaaa tgacgggcaa gaaaggcttt ctcttcctca actgtcatat atgcatggtt
     4441 acgaccacca cgtgtttctt gaaggagaga gtcgagtccg aactcctcat atttttttac
     4501 gtttcgccaa atcgttgttt gattacagtc taaaagctct ataatctctt tataagattt
     4561 gcccatcaga cgaaatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca
     4621 ttgttagaaa tcgatttgtc ctgttcttgt ttcattttac tatagaacga tttgaaggcg
     4681 tttataatat ttagctgtac gagagtcttt taaaagtgtt ttgatggttt ggatttcttc
     4741 tttagttgat ttcatattac tattatatgg tgctttttta ttttagtctg gtataaatag
     4801 cgagaaatat ctatcctatc ttctagaatg tcttccaaac gagaaaactc tcgtaaacaa
     4861 agaggtttta gaggcctatt taccgtagac taaagttgta caagaaaagt gcaaataaga
     4921 aatctccaga ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatagacttc
     4981 attattggac ggttaccaag ttactcaaag tacagatttg aagctgcaga taagactgac
     5041 gaaatcactt taatactaaa tgaaaatcaa aaagcaaact aagaagctag cctcaggctg
     5101 tacttgagta cggtaaggcg acgctgacgt gatttgaaga gattttcgaa gagtataaaa
     5161 cactgttttg aggtggtgga taaatcggct gggcaaaaac tcaatctcag gagaaaatga
     5221 agtaaatctc cccacaacta tagaaaaaga tttgattctg ctcctcacac aataaaatct
     5281 ttttctttta ttaaaaacga atcgtttcac gtgttttctc atatgaggta acaaagcgat
     5341 tggttacacc aggctctgcc acttccaatg cttgagaaat cttgtcaaat gaagcgtgat
     5401 aatcaaattc tttttcaaaa atatctaaag cttcgttaaa tgcttcttga atgcgttcat
     5461 caaatgagcg atagcggtta gaatattgca agagttgctc tgtcaaagtt gcatattgta
     5521 caatattata agtttccgtt tctaaagctt ccatatcatt cgttgcaatt tcaagaactc
     5581 gggtaacaga ttcaatgtta atcatttttt gttctaactc aaccattaaa tcctcggtat
     5641 tattgcttgc cgtaaagaat aacttcaaga aagtttgtgg aatacctggc agattgcgtt
     5701 tttccatgta tcgcttgata gtatggagac gattgacata aacattggcc ttttgacgtg
     5761 cattaatatc atctttctca atttgtgtca ggcgctcact aactgaaatt tgctcatctt
     5821 caatatcttt tagttgagtt tgtaaatcct caagattttc ttcaagaact gaataagctt
     5881 gggttggttc ttcttgattt gaagttacct caacaatagc tgcctcaaaa ctctctaatt
     5941 ctgtctgaat acgacgaaca tggcttgcag ctgtctcagg aagtaaatag gtcttgttca
     6001 aacgtgcaat atcttctccc aataaagtat tattctcttt catatgttga aggtaagttg
     6061 gaagagttgc aagtagattt tccactactt tctgagcagc aatttctcga gtaaaaatat
     6121 catacaaggc attgatttcc tcttgtgcct gtccattctc atattcggca ttatccaatt
     6181 ccaactgacg aatattctct tggtttttct tgaatgcttc ataaagcaag tggaaacgcg
     6241 cttcaatatc cgtttcaaca aaatgataat tagcatcaat tagtttacga taaccggctt
     6301 ccaaatcctg taattgatct ggcaattctg tagaaagcgt cgtaaccaag gctggaacac
     6361 gatccacaat atgacttaag gccaaaatgt gattttctgt attatccaaa atcactgcgg
     6421 cttccacagg gtcacccgat gaattcaagg ttacaaattg tgaaaattca gattggatat
     6481 tttctaattg tttttcaatt tcatccaagg cttgaccata ctgttctgaa ttttcagcaa
     6541 ctctatgctg aagttcctca aataaatcca aagcatgaag aacacgacca ctatttttag
     6601 attcttgctt ctctaagtct gccaaagcat tgcgaattgc cgcaatatct tcttcaatca
     6661 aagtaatttg actctcaatt tggtcaattt gatgactggc cttgagaaaa cgaaatgaat
     6721 ggttatagcc ttctgcttca aagagattat tttcaatatc ggcaaaagag ttgagagata
     6781 aatcgaccca tttttgattc cattcacgga aagccacttg actttgtcca atcaagtgca
     6841 tattttttac agcttctact tcatcattta ctggaagatt gtatagttct tcttttcttt
     6901 cttctagcgc ctctaatctc ccctcgtttc gcttacgtag aaagattgcc actacataag
     6961 ccagaactaa aatgactgca attgcaacca ttaaataaat tagttgtcca ttagacatat
     7021 caaactcctt ttttacttga aacaatcgta acgattatat catatttttt agaccaaggg
     7081 aactacttct ccctattttt tagacatcaa gtgtactata gacagcattt tcttcgataa
     7141 actcacgacg aggctctact cgatccccca tcaacatatc aaagatttta tctgcttctg
     7201 cagcatcatc tacagaaact ctagccatca agcgatgttc gggatccatg gttgtttccc
     7261 acagctgatg atcgtccatt tcacctagcc ccttataacg ctgaatagtc ggtttggtac
     7321 gaccttcact ataacgggct aaagcttctt ggagtttgat ttcttgatct gcacccggct
     7381 ggatatattc tttaatctcg cttccaacct tgacaccata gattggtggt tgggcaatat
     7441 aaacatagcc agcttctagg attggtttca tataacgata aatcaaggtt aaaagaaggg
     7501 tacgaatgtg ggctccatcg acatcggcat cggtcatcaa aacgagtttt tggtaacggg
     7561 ctttcgaaac atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa
     7621 tttcttcgtt agctagaatc ttatccatac ttgctttttc aacgttcaaa atcttaccgc
     7681 gaattggaag gatagcctga aactcacggt tacgaccaga tttggctgat ccaccagctg
     7741 agtctccttc gacgatgaag agttctgttt cagcagggtt attagaagaa cagtctgcta
     7801 gtttccctgg aaggttggaa atttccaaac cagatttttt acgtgtgact tcacgcgcac
     7861 gcttggcagc cacacgagcc ttggcagcca aaattccttt ttctacgata cgtttggcaa
     7921 tctgtggatt ttccatgagg aaatcggaga aggcttcact gaagaggcga ttggtaatct
     7981 tgaccacttc gctatttccc aatttggtct tggtttgtcc ttcaaactgt ggatttgggt
     8041 gtttaactga gataactgca gttaagcctt cgcgaacatc ttcccctgtt agattgtctt
     8101 cattgtcttt cagtaactta tttttacgag cataatcgtt gataacacgt gtcaaggctg
     8161 tacggaaacc ttgttcatgc gttccacctt catgtgtatg aatattattg gcgaaactca
     8221 tgacattttc atggtaaccc gttgtgtact gcatggctac ctcaactgtg atatcatcca
     8281 tctcaccgtc tgtatagatt ggtgtatcaa agattacatc cttgttctcg ttgatatatt
     8341 caacgtaact agcaatccca ccttcataat gataatgctt ggtttgttcc aaaccttggc
     8401 gcttatcagt gatagaaatt tgaagaccgc gatttagaaa ggccaactct tgaatccgtt
     8461 tatttaattt atcaaaatca aagattgttg tttcagtgaa gatttttggg tccggtgtga
     8521 agtgaacagt tgttcctgtt ttatccgtat ctccaactat ttcaagatct gcgacaacat
     8581 gaccacgacg gtattcttgg taatgaatct taccgttttt atggacatga acgtctaatt
     8641 gagtggaaag ggcattaaca actgacgacc ccaccccgtg aagaccacct gaaaccttgt
     8701 atccaccacc gccgaacttt cctccagcgt gaaggactgt aaagacggtc tcaacagcag
     8761 gacgacctgt tttttcctga atatcgactg ggataccacg cccatcatcc acaacagtaa
     8821 tcgaatcatc tggctcaata aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt
     8881 caattgagtt atcaacaatt tcccagacta gatggtgaag accttctttt gaggttgatc
     8941 caatgtacat ccctggacgc atacgaacag cctctaagcc ctctaaaact tgaatttgac
     9001 tggcatcata atcctgtgcc tgcagatttt tgatttcttc tgtcatctta ttcctttttc
     9061 ttatatgtct aatacttgtc ttaaattcac gatactggta aacaagtggg tatcaagtcc
     9121 tgcagcttga cctgcttcga tatcaatcgg ccgatcacca atgacaagac cagagctaat
     9181 ctgatacttt tctcttaaat aaagcatgga ttcgggattt ggctttctct taaagcctga
     9241 gctagaagtc accacttctg taaaataagc tgctatagag gttttttcta aaatttccaa
     9301 aacctgatca tttcgatgag agaccaaaaa atgacggcca ccttgattta aaatgtcttc
     9361 caataggtca gaaactcctt caaataaaat cgggtgttca agctctctgg cttcattttc
     9421 cttgtacttt tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg caaaaggagt
     9481 agaaaccttt aaagcttgat agacactgtc atggtcttgt gtgataccat acagtgccaa
     9541 tgtttcaaca aatgcagctg ttgaagtttc ataattatcc agtaaagttc cacctaaatc
     9601 ccagatgtaa tcgtgatatt tcataccctt cattatagca tttttttatg aaaaaagcga
     9661 tgatttccct gagttttcca cataaaaaac gagagaataa ctcccgtttt actgattttt
     9721 accaaagaca tctcgataga gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg
     9781 ctccaccaac tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg ttgtcaactg
     9841 accatctacc actactgttt ccttgacgta gtgaccatca aggatttgct cttgaacgcc
     9901 gtcataacaa gtgtatcgct tatttttcaa tatctctgct tgattgaggg caattggtgc
     9961 cgcacaaatg gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa
    10021 ggtctgatta tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa caatcatatc
    10081 atagtctgat aaatctccat caaagacatg atctgctctt acttggattg catgcgaacc
    10141 cgttacttgc tcttcaaaac caaccatatc acatgtgata ttggctcgac gcaagacatc
    10201 tacaactgtc aaggcttcaa tttcttcaaa gccctgagct aacataactg ctacttttac
    10261 catgattttc tccttattta atttgtttta atacaacctt tttccgtttg aatgggactt
    10321 ttccgctctt ttcttcagct agattagtct ggtaactcat cgataaaagc aaaccaacac
    10381 caatcagatt actgataata gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg
    10441 tcaaaggaag tagtccagtc acagcaccga tattctcaaa gatgtggaag agcaacatca
    10501 taatcaaacc tgtggaaata taagtgtaga actggttatt tgatttaaga gtaatcttca
    10561 acatacggta aatcaacatg agatagaggg caataaccag gacagagcca ataaagccaa
    10621 aatcttctgc aataaccgta aaaatcatat ctgactctcg aactgggata agcagattcg
    10681 aagcattaaa tccctgacca aataagccac cactcccaat ggcaatctgc ccttgagcct
    10741 gctggtaagt cgttgtttgg gcaaactcaa agggattgag ccaagccaaa atccgattga
    10801 tttggtaggt cggcattcca atctggtgaa gaaaagctcg tccgtccttg ctaataaaga
    10861 tagctaagaa accagcaact cctgttacag cagtcacaaa tactgggata ataattttcc
    10921 aagaaacccc tgataataaa acgattcctg agaaaatggc tacaaaaacc aaagccgtcc
    10981 ccaagtcact ttgaagtgct aaaagaacta ggactggaat ggtaaagaga atcatccaga
    11041 aaattaacaa aaagtccagc gaaaccgtgc gtctccattc cttatgtttc tttgtaaatt
    11101 ggacaatgac acgagccaac atgaggatat aggatatctt cataaattct gacggttgaa
    11161 atagggtaat tccatttatt gatacccagt ttttggcacc cgttgatgca actaagcttg
    11221 gattataaaa tacaatcggc aagatcataa gtcccaagcc taaaatatat agaaaggggg
    11281 tcaccttcca aagaaattct gtattaaaga gcatgaccac aaaaccaatc acaagcccca
    11341 aggcaatcca ggcgacctgc tgccctaaaa tgggcagaat attattggga taatcatgac
    11401 taacggctat atagatagcc accacaccga tgaccagtag aaaaaatact ggcaagagca
    11461 aactgtaatc gactcttgag tcgagagaac gtttcatata tactaacctt atactttcat
    11521 acaatactat ttatcaaagt tcattaaaaa atctatcaat agcctcgtca acttcggatc
    11581 gagagatggt tttaacagtc gcttcttctg ctagagatgc tactatttgt ttgccgtatc
    11641 gttttccgac gattctccta tccaaaataa gagttaagga acgttggtat tcacgtctca
    11701 tacttcttcc caaagcctgt tttaaacgaa taatggccat tggcaattga taatcataaa
    11761 aggcattttt cccttcttga ttcagttctt gattaatctt tttcgtcaag ggttcttgag
    11821 gattttggaa aggaagcctc ggtacaactt gaatcacaga aggatggctt gaaaaatcaa
    11881 ctccctccca gaaacttgct gcaccaagca agatttgttg ttcacctttt tcaaagcgtt
    11941 tctttagctg atgaacatcc ccatttttat actgggccaa gtggctaact gtaagtaaat
    12001 ccgatactgc tagaagcatg tctttagcgg taaagagaac caaaatcggt tgctggaaag
    12061 cttgaatttc cactagtaaa gcagctacct ctctggcata gacttctaag gaggtttctg
    12121 ttaccagagg gaaatctttg accatgacca cttcttgttc ctgttttccc cgagattcaa
    12181 tcttgacaaa tttagcttca ggatagccta aaaggtctgc caaagaaacc ctctgactaa
    12241 tctcaagagt agccgatact cccaagactt ggcaactctc aggaagtaaa gaggacagaa
    12301 gagtacggcc tgattttgta gaagaaatct gaattttctt ttgactcgtt tcagttactt
    12361 caagccagta atcaccttca gctgtaaaat agcgaaccag ctcatcaaag tcttctactt
    12421 ccaattctga aaaatactga tggagattgt ccagagaatc taagatattt ttcctagatt
    12481 tgccagactg aaattgttct atcaagtaga gacactcaaa gcgaatactt tctagtatcc
    12541 gttgttgaac cctgttttct tctcctacta aagccttatc aattaaatcg ataatagatt
    12601 gtatatcgta ggtctcttga agcagatttt ctagagctaa caaaatcttt tggacttcat
    12661 caataatcag taaacggtca ctgacaaatt caggattatc ttcaagtctg gttacgagat
    12721 aggcatgatt agtcactaaa agcttgcaag tctctgccct ttcttgacta cgtttccaaa
    12781 aatcttccgt cacaaataag ctctgggatg ataaattccc atcatgacga aggtctgcta
    12841 gaaaatgttg gtaacggtag agttgcccga tttcatccaa atctcctgtc tctgtctcag
    12901 taagccagac caagacttgc attttaaagc gtctaaataa gcgattttca tcattttcct
    12961 gcaaggaatg ataaaaggca tccaacttca gataattttg tggtcccttt aagctatgaa
    13021 tatctgtatg gaacacttcc ttgaggcgtt taccttcttc ttccatgatt tgattttgaa
    13081 gaatctttgt cggaacacta agaacaattt gtcgctcttt ggattgagat aaagcgggta
    13141 agagatagcc ataggttttc ccaatccctg tcggcgcttg aatcagagag acaggttcat
    13201 ctttcaatag caagccaacc tctttagcaa aactttcttg ttcctccctc acttcaaggt
    13261 tcaacagaga aatattttta gaaaagtctt gagatagttt tcgtagctcc agagaagctg
    13321 ccgttttctt aaaatacaga ccttgaactt ggaccaagtc tggagaactc aggatagatt
    13381 ggttgcgata agtttcctca ataaccaggt aggactcata taggagagcg tcagccattt
    13441 ccagcaagcg ttccaagaga cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa
    13501 gtaattctgc tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc
    13561 ctaattctcg acacaaaatc ggcaagctat atttttccag ttcagggaaa aagacctggg
    13621 ccaattcgac cgtatcaaca cgagggtttc ttagctcata gccttcaaaa aataaatttt
    13681 ccgccaagag attagcatca aactgaacat tatgggctac aaaaatccca tcctccacca
    13741 agtcaaatat ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg
    13801 tcaatcctgt cagttctttg atatgagcat ccaagggttc atgtggattg acatccgtcg
    13861 tatagtgatc gacgatttct ccatcctcaa tcacgacaat tcccacttgg ataattttag
    13921 ccttactacc tgtgctagtt gcctctaaat ccaccacaac atagcgatta ccaatcgttt
    13981 tcattataaa aaattatacc aaaaaaaggg ccatttggca cctgcaaaca taggataatt
    14041 ttcaaactca agaacactac ttttcttaaa aatcccatcg aatagagcat tctagcctgc
    14101 tgatttttgt aaaatagtga agaaatacga aaaattgaga gaagaaatgt ttgcatcttc
    14161 cttctctcaa cgaatcatgt attttatcta accatgtcag gatcgtagtc ataaaatcct
    14221 gtatcaagga aacggttttt agggtgaagt ttatgaactt cttcatccag caagaaggct
    14281 tgatcatggc taaagttgcc ctcttgaatc aaattacgcg cttggataaa gagttttgca
    14341 atctcaacaa agttctgacc aggagtgtag agcccttcta gtttccagcg agtaaagcca
    14401 tgctccacca attctgttaa tttgatcatc aaatcaaggt cattgttggc aaagatatgg
    14461 gtcccatgat tatcttcaaa aatggaatag tggctctctg gatcacttgg ctcagccaag
    14521 aagaggtcac gtttatgcgt cttttcatca tcgatatgtg taaagttata gtagttttgc
    14581 aagagtggac gtttagaatg atggatgacg ctagcaccgt aaaccaaaac ttcagcagga
    14641 atttccaaaa tctctggcat tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca
    14701 gcctcagatg cgccagcctt ttgtccccag aagttaatct gacgactgct agttaccatg
    14761 gttgaagcat cgtagatggt cttaaatgaa taaccatcgc ggttaactac gtaaaagacg
    14821 cctgcatccc caatcgtaat atagtctgtc ttgatttctt ccaagaagtc taagaaaggc
    14881 ttgatacggt ccatcatatc ttggtgcatg agagcattga ccgcaacgat caattcctta
    14941 ccagcatcat gaaccaactt agcgatttca cgtaattggt cataactaaa ggtcgttggc
    15001 agacgaagac caaaatcttt ctcaccgaca tagatacggt ctacgccagc ttcgagtagt
    15061 tgttcaactt gttcaatact ttcagcagtt gctgtaatga caatcttttc cataggaaaa
    15121 attataccac atttctccaa aatcagccat gctttgaaaa tgaaaaggga ctttattctt
    15181 tttgtaacct ttctgtaata attctctgcc gaaaaaatga taaaataggt atgtactgtt
    15241 aaggagagaa tcatgcccgt aagaaaatta caatcctatg aggtagacta tcaagaagaa
    15301 ttaaaccagc agcttcctca ttaccaagct tatacacctg aagcgcaatc tgatgccaat
    15361 ctcaaggaaa tcctattttt tattaatatc gctgtttttt gtatctgtat tgctatcttt
    15421 agttttatct ttttagcatt aaaattatca actgctcttg cctttgccgc agcaatcgga
    15481 ttcagcttac ttgttttaaa agttcaacgg tctattatca aacgaaaacg tagaagataa
    15541 atccaaaata tcgccccaat tggagcgata tttttatttt ttctttttag atggtgtctg
    15601 aatggcaatc ttcacttcaa agattgttcc cttgggttta ttatctttga cagtaacagt
    15661 tccttttaga gcatctacaa tttgcttggc tagggataat cctaaaccaa aaccaccttt
    15721 ttgccgggtt ctagccttgt ctactcgata aaaacggtca aaaattttct ttttatcttc
    15781 tgtcgaaata ccgattccat tatcagaaac aagtaaataa agattgcgat cggtcgccga
    15841 gataagaaaa tcaatttcac catcctcctc agtatacttg acggcattat cgaaaagaat
    15901 ggtcatcagt tgtttcagaa gaagctgatc tgtgacaatt gttcgatgga tacgattttc
    15961 aaaacggaag acacgattat tttccgaagc aatcatctca tagtttgtga aagttgtatt
    16021 aaaaaagcta gttggaactt ctgcaagctc cggcttaatc ccatcatctc tccgagctaa
    16081 gttcagcaag cttgtcgtta aaaaacgcat atttcggact tcttccaaac tcgatgcaat
    16141 gctttcgctc acatccataa tggtagcttc tggcttacga aaaagggtct ctaagcgatt
    16201 ttgcaaaact gcgagtggag ttcgtaactc atgactggca ttttccacaa aagactgttg
    16261 cttctgcata ctctcaagca ggggcctaac actgacccta gctagataga gactggcaag
    16321 taaagacaaa atccagaaac tagccatcac gaccacaatc aattgctcat gcttttgact
    16381 agcctgctcc aactgactgg tattaatcaa gacagcagca tacttgatat tggttgaaac
    16441 cgaactaata ttggtctcca tcaaaatcac acgatagatt tcttcctgcc catagctatt
    16501 aaaaacctga atctggtaga tatgtcctag ttctttcttt tctaacttaa tcttatccaa
    16561 gcccaaaaat cgatttccag aaagaagttg agtaaagtct ttatcaaaga gaatgacttc
    16621 cgtattggaa ctgacattag gttttatttc tactttacta gcgtccgccc tagcattttc
    16681 taaatcttta atctcttctg ttgccctatt tatagccagc tgaataactg cttgaggatt
    16741 ttcactcagt ccatgaagct tatcgtccac cgaagtataa agactcgaat gcatgacttg
    16801 taaaataatc agagtcattg tagaaaaaat cagggtgaag acaccgaagt tgcggataaa
    16861 ataactaaag tcatccgcat accatgtttt tttaagttta ctgaacatct tttaaaagat
    16921 acccaacact acgcaaagtt tgcaaattct ctgcaaaagt ggttcccttt aatttcttac
    16981 ggacttttga aacatagact tcgacaaccg aaatcgttgt atcactatca aatccccata
    17041 gacggtcaaa aatctgcgtc ttaggcaaaa tcacattttg attttgaagg aaataaacta
    17101 gtaagtcgaa ctctttcccc agcaattcga caggagtatc ttcaacttta acggtattgg
    17161 ttgataaatt aaccacgata ttcccataag tcaaggtgtt ttcattaaac ttccctgaac
    17221 gtttgagaag ggcctgaatc cgcattttaa gttcttctag gtagaaaggt ttggtcagat
    17281 aatcatccgc tcccagttca aatccatgtc ccttgtcatc caaactttcc ttggcagtca
    17341 taatcagaac tggtgtcgta attccctttt cacgcaattc ttttaagact tggaaaccat
    17401 ttttttctgg caacatcaaa tccagcaaaa tcaagtcata gacaccactc tcagcttcgt
    17461 agagaccttc ttctccatca aatacctgca taacatccgc aaaatcgtct aaaaagtcaa
    17521 atactgaatt tgacagacct aggtcatcct caaccaataa gatttttatc atgagaaact
    17581 cctccttatt aaaactatta taccaaattt gccttaaaaa aaactcaact ctctgcattt
    17641 tacatgagat agctgagttt tctttttatt ttaggcttat ttatgcattt ccgtattgaa
    17701 gaacaactgc ttcgactgca gctttttcac ggctaatcaa gtcaacacgt gctgcaattt
    17761 ccttgattcc cataccgatg ttacggctaa gagcaaggtc agaaagttgc ggctcaaaga
    17821 actccttgta ttccgccaag cgttgctgag tcttaaatac atgagcagga aggataacaa
    17881 agctatcaaa gctcatatct cctccaaggg ctgccttaat ccaagcccag ttttcacgcg
    17941 cccaagacca agctgttttc tgagttgctt gatgagctag gaattggtaa taccaagcag
    18001 acaagtcctg tggtttgacc acaaatttgt ccttccaaga agtaatcagg ttttggatat
    18061 tatccgcatc tgtactgtat gcaagagctg ctgccaactg gcgtttaaag acagcatctg
    18121 ttgcgtgagt ataagtatca agataaagtg ctaacaagtc tttagtctca tgatgtttca
    18181 tctcattaat cagaacttgt gagcgaatag ctgctgggag tcctgcaaga ttctccttgt
    18241 gtgttgcgaa gatttggcta gcgacttgac tagcttctgc atcatttgag cgaatcatca
    18301 tcgaaacagc cagctgacga accaattcat cctcatctga ttctccgtct ttagcttcaa
    18361 aaccaagacg gtcatagtta tgacgagcca atttagcaac cagtcctttg aaggctgttt
    18421 cagcatccgt tccttcatca ataaagcgct caagggctga aatcacttga gaaacagctg
    18481 aaaccaccag ataagactct tccttagcaa gtttatcaag aactggaagc aagtctgcat
    18541 aagaaatgtg ccctgcctca gccaacaaac gacgttcttg aacaatttgc agtttgcttg
    18601 tgttatcaag tgtctctagc tcagcaagaa cagcttctaa caagtctcct tgatagtcgg
    18661 taatatagtg ggcagtattt tcagtgttga gacgaagagc tccttcattt tcagcaagaa
    18721 gagctgcgta gccagggatt tcgatacttt cagtttcgag tgtatcaggc aagcctttcc
    18781 agttgctatt gaggggcacc acccagagac ggttcttgtc ttcgttctca ccgatgaaga
    18841 attgtttttg tgaaatcttc aagacatcat tttcaacttt gacagtaaga actgggtaac
    18901 caggctgttc caaccaagaa tccatgaagg ctgcgacatc acgtcctgac gcttgaccaa
    18961 gggcatccca aaggtcacta ccaatggtgt tgctgtattg gtgtttttca aagtaggcgt
    19021 gcaaaccttt agcaaaatca gcatctccta gccaacggcg aagcatgtgc atgagacggc
    19081 ttcctttggc atagacgata gcgccgtcaa agagtgtatt gatttcatct ggatgtttaa
    19141 cttcgacgtg gacagactga acgccatcag tagcgtcacg ttcaagagca agaggtactc
    19201 cacctgtttg gaaatcttca aagatattcc agcttggttc gatggtatcc acacagacgt
    19261 attccatcat attagcgaaa ctttcattga gccaaaggtc atcccaccat ttcatagtca
    19321 cgaggttccc aaaccattgg tgagccaatt catgggccac aacaagggca acttgttgac
    19381 ggctagcaaa tgtagagttc tcatcgacaa ccaagtaaac ttcacggtag gtcacaagac
    19441 cccagttttc catagcacca gctgagaagt caggaagggc gatgtggaga gattgaggaa
    19501 ttgggtactt aactccatag taatcttcgt aaaactcgat agagcgaaca gcgatatcca
    19561 gtgagaaatc aagatttgaa agtggatgtg ctttggttga gtagacacct accagggtac
    19621 catttttagt tttagcggtc accccttgca aatcaccagc aacaaaggcc aacaagtaag
    19681 aagacatgcg aggtgttgtc tcaaacttcc agatacctgt ttccttacgg ttttcaacat
    19741 cgatttctgg catgtttgac aaggccaatt caccttctgc ttggtcaaag cgaagagaga
    19801 ggtcaaaagt tgctttggct tcaggctcat ccacacatgg gaaagcttcg cgcgcaaaat
    19861 ggctctcgaa ctgagtagac aagacctcct tcttgactcc atcaactgta taataagaag
    19921 ggtaaatccc tgtcatgttg tctgtaattt taccagaaaa ggcaagaacc aattcaactt
    19981 gaccagcctc agccaattcg atatgaaggg cttcattgtc atggtcaact gtaaatggac
    20041 gagcttgacc tgcaacttct acagaggtga tttccaagtc tttttggtgg agggagatgc
    20101 ggtcactctg tgcttgacca gtgatggtca ctttcccaga aaaagtcttg gtctcacgac
    20161 tcaaatctaa aaataaatca taatgttcag gaacaaattg cttaataaaa tgttcaactg
    20221 cttgcatagt tttctcctat tctaagttta agagctgctg ctcttcttca aacaaactta
    20281 ttatatcata ttttgcttga gaaaaaagga ttggacagcg atttccaaaa ctttcttcct
    20341 tctaatagtc tacagtgggt agaccttgcg aaattcttct aaaacaacct tgctttcagg
    20401 tgtaaaagtc agccctactt cacccaaaca gtctgtgaaa aagtcctcat gaacctggcg
    20461 ccaataggca agtgatttgt ctccctcacc ttccttaaag gcatgttgcg cagaaacttg
    20521 attgaagagt tcaacagaaa cctttgtaat ttcgacaacg cagacagcct gattttgact
    20581 atctaaaatg acatcgaagg tccctacttg gggaagtggt tcgtcttcta gtacatagag
    20641 gtcataggct gatgctgttg ctgtcttttc tcctttaaac accaaatccg ctaaaaggtc
    20701 tggttcaact ccaaaagccc aagcatcgat ttcatctccg atcaaaggat tgatttgctt
    20761 gtatttattc cacatttctt gcggtatcat gggtactcct ttgtaatttt ttactttctt
    20821 cttttatgtg tttaagatga tctggatggt caatctctaa atcaaaaatc tctggaatag
    20881 aactgtagtg gataatgcac ttgacaccca actgattcat tttttgtatg aaagaagtat
    20941 tcagatagcc tgctacagca aaattaatct tgttctttct tgctttatcc tgcatatctc
    21001 ttagcatatc taacattatt ggactttcca tatcatgcca ttgactgttt ctcatagtcg
    21061 caaaaacaaa ggaagtcaaa tcattcattc caactacaat ctttgaaatg cccgtttcca
    21121 gtatactaga taagtcaaaa tacgctgacg gtaattcaat catcgttccg actttcccag
    21181 taaagccata ctgacgcaat actgtaatag cttgttttaa ttggtcggca tcattgacaa
    21241 aaggaaaaat aagagacaga ttgggattgg tttgataaac ttctgtaacg acatgtgatt
    21301 cagcctgaaa ttcatccaaa cacgccagta aacgcctggt tcctctatag ccaaacaagg
    21361 gatgcccttc gtcaaaaaac tctctagtcc ccgctaaaca attggcttct gtattcgtta
    21421 attcagaaaa acgataccaa acttccttat ctaagtaaaa ggagcaaata gtatcaagat
    21481 aatcttttac aaattgctga caacttgcta atatgatgtt ttgattaagc tctctcaata
    21541 agtattcccc acgaatcatg ccgacgtggt gaaatagttg aggataaatt ttttcaagaa
    21601 ttttttcgcc actaagggca agttgatttc tcatagccat tcacctcgtc ttaaaagata
    21661 caatatcata tataattata tcaaaatttc ctctagttca aaagcttcat aaggttcaga
    21721 taactcatat cctaaaaatt ctgctacttt tttggaagcc tcattatgag catcccaaag
    21781 tggaaacata tctctattta aactctctag aatcattgca gcaccaagct ttttagcaaa
    21841 tccatttcct tgttcgtttg gtctagttgc aacttccact tcaactgctt tacggtaaac
    21901 taaccctgag gaaatcccag caatcagttc attattttta agaatcacaa agccaaatcc
    21961 accttttaaa acaaaatcct gataggactc aaaatccccc tgtaaatcct gtgaccattc
    22021 ttccgtagaa aagcagttat aaatatgatt atcaataggc acaatattgt aaccttcctc
    22081 taaatgagta actagattat ttaaaaattc aacttgaaaa tttgccttat ctttaaaaga
    22141 atagcgagta aaagaattta gccccacttt tcgatgcaga aaatcttgcc aattagtatt
    22201 ttctgaaata ataatcttat aatctaaacc atattttcta acaaaatctt cccaaaatct
    22261 ggaatccaac tcccctgcta ggtatagaaa attcccaaca tcataaaagc ttgttgtcct
    22321 atcattgttt cggtagacag ttccaatttg agatttaaga ccatagagaa ccatattttt
    22381 cttccatgtt ttaaatagtt ccattatccc tctacttcac cttccaattc atgtaagaag
    22441 tcttgtccag ttctggaaat cctaataatt cagacttaac cttcaagact aatggcgatg
    22501 cattttcttc tgtaatctct tgaatatcca tccaaatata tccaagtgaa tcattcgcac
    22561 catcagacac agcttccgaa gtcgtaactt gaggtgcact ctcattcatt tcaacatcat
    22621 acaaggccat gacatggtga accataaaat ttgttaactc ttctctgacg aaaacatcgt
    22681 agattcgagg attagagtag cttctaacag taaatcccgt ctcttccata acttctctag
    22741 tcagcgtttc cgtcagtcct tcaccaagtt gctgactgcc tccaggtaga tcataccgat
    22801 gttgataagg gcctctcgct ttttcaatgc aaagtaactt cccattttct aagcaaacag
    22861 cgtagactcc aaagtgtttt ttgatttcca tccaactcct cctacttcaa agaccagcca
    22921 ccatctattg tcaagatttg tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga
    22981 ctaagctctg ctatttcctc tggctcaatc cagcgcttga ttggggtttc actagccacc
    23041 cagtcagcca aaccacctgg ttcaaaatcc gcagcggtca tagctgtctt gactgctcct
    23101 ggagcgatcc caaagacctg aatcccagct tcagcatagt ctagagccaa ctgcttggtg
    23161 aagccagcca aggcatgctt ggatgaagta taggcgtgac cacctccacc tgctaggcta
    23221 gaagcaatgg aacacatatt gatgatgatt ccctttttat tttccagcat ttgtgtcaaa
    23281 taataccgag tcaactctac tggaataatg tagttgattt caaaaatctc ttgaatgtcc
    23341 tgcgccgttt gttccaacag tggtttgtaa tcatccaaaa ctccggcagt attacacaaa
    23401 acatccacct gagggcacca gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt
    23461 aaaaagtgaa aatcaccctc taagagtggc ttttcacctt ggtcaactcc ataaacttga
    23521 tagcccttct ctaaaaagag gcgagcttga gccaatccga tccctgaact cactcctgta
    23581 atgagtacac gtttagtcat gcacttctac ccaatccgtt gccaaaacat cacaagctgt
    23641 cgggctccac atggaaaaac cttctccttc tccagacacg ttgattagga aatagggtgt
    23701 cacttcaagt gcaagcccat tttgctcgat ggtatcaaag agttggacat agttttcagc
    23761 acctccccaa ccagttcgta catatttttt cttagccttt aagccaggta gaatttcttc
    23821 aaatgtcatg tttttctcct ttaatgtcaa taattctccc cttcattata acaaaaaaac
    23881 cgctttaaaa cggctttttg actgtgattt atttacatct tcttctactt acggcaaatt
    23941 attccctgca gcaagataaa tttcatacca ttcttttctt gttaagctaa agtttgccgc
    24001 ttggctaact tctctcaagt gcttaggatt tgtggtacct acaactgcct gcatttttgc
    24061 tggataacgc aatatccaag aaatggcaat agttgaagag gttactccat atttaatagc
    24121 taaacgatca agtacttgat ttaaagcttg aaatttctca tttccaacaa aattcccttt
    24181 aaaatacccg aattgtaaga cagaccatgc ttgaatgacc acatcgtgta atttgcaata
    24241 ttcaaaaatg ctgccatctc gcatagctgc ttgactatct tccatattaa catgaaaagc
    24301 tgattcaaat cctggagtaa aagccgcact caattgtagc tgattaacag ctaacggctg
    24361 cttgacatct tttttaagca actccatcat cataggattt tgattagaaa ctccaaaatc
    24421 tcgaacttta ccttgtttat aaaggagatc aaaggcttct gctacttggt cagattccat
    24481 caaagcatct ggtcgatgaa ggagcaagct atctagatga tcaatcttca atctttgcaa
    24541 aataccgtct actgatttta taatatagtc cttagaaaaa tcaaaatagg taaattcttc
    24601 aatgcgaatg ccacatttgg actgaatcca catcttttct cttaaatctg gacgattttt
    24661 taggacaaga cctaacagtt cttcacaacg accacgacca taaatatcag ccaagtcgaa
    24721 ggcattgatt ccaacagaaa gtgctgtttc tacaagctct tcaacttctt ttacagactt
    24781 atcttttatt ctcatcattc cgagaacaat ttctgataat tctttgtcat cttgaccaag
    24841 agttatgtat ctcatcaaat ttttctcctt taatttctaa cattcttccc ttcattataa
    24901 caaaaaaccg ctttgcaacg gctttttgac tatacttcac tccattttat cttcttaaac
    24961 ccacggaaca agacaaagat tccaataaag aggacagcta aaggaataac ttttgtaagg
    25021 aaaacatttg aaattcccat ccactcataa taacggagca gagaacccac cacaagatgg
    25081 gcaataatca tactgacaaa tggacgaaag accgcttctt tccaattcca aataccgata
    25141 actagcgaaa tcgtaaagac agacaaacta tcccagggag ccggaatata aaaggctcct
    25201 tcttgtatga agcttgccat tcctacatat cctaaaacaa ctagaagaac tatagtccca
    25261 acaacaatgt aagtgccaat tttcatttta ggagaatctt ggactaaact tcttcgtaaa
    25321 attgtggcca caagtccaaa tccaatcaga aaaataagaa gttgccctaa aaatgtgagc
    25381 aaattgactg ttaagagagg acctttagaa aaatcactta gtagttgata ataacgtaat
    25441 accgccagga caagaattgg cgtcaaaagg gactctttga tagaactgcg aggtgctccc
    25501 ttgagaatct ctttcattat ttttttagga ttcttaccta gataatcctc tgcactcatg
    25561 ccatctcgtt ctgcttctga gaaatctagc atcatcaaat agatctgctc tctgagatag
    25621 tcttcatcat agagaaatcc agcaagatta aaactttccc acaactcctc aaaatacttt
    25681 tgattctcct cagaaaactc atgtagcaaa gcgcttgttt cttcgtaata cttcattttc
    25741 ttcatggttt aacccccatt cttaatccct tctacttttt gactcaaatc gtcccattgt
    25801 tgccaaaaga ctgagacacg ctcttctcct tctttcatta atgaaaaata cttccgatct
    25861 ggaccatctg gcgacgggcg catgtcgcct cttatccatt gatttttttc taacttttgc
    25921 aacaaaggat aaatagttcc tggaacgata gtatcaaatc cagcctctcg caaagtctga
    25981 accaactcat aaccataccg ctctttttga ccaatcatat ccaagacaca accttcaaga
    26041 acacctttta atagctgagt ttctttcatc acttctccct tctaatctat tttgtaatac
    26101 ctactagtaa cttcacctat agtatattac ttctacacta gtttgtaaag cataatagtt
    26161 aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cctaccgtat gtatggtt
//
LOCUS       DAVMHG010000013        26052 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMHG010000013 DAVMHG010000000
VERSION     DAVMHG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.013"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            95..691
                     /locus_tag="VYR08_000440"
                     /pseudo
     CDS             95..691
                     /locus_tag="VYR08_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            767..1216
                     /locus_tag="VYR08_000441"
                     /pseudo
     CDS             767..1216
                     /locus_tag="VYR08_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1229..2320
                     /locus_tag="VYR08_000442"
     CDS             1229..2320
                     /locus_tag="VYR08_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466019.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2310..3945
                     /locus_tag="VYR08_000443"
                     /pseudo
     CDS             2310..3945
                     /locus_tag="VYR08_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4048..4740)
                     /locus_tag="VYR08_000444"
     CDS             complement(4048..4740)
                     /locus_tag="VYR08_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3466020.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(4984..6321)
                     /locus_tag="VYR08_000445"
     CDS             complement(4984..6321)
                     /locus_tag="VYR08_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV3466021.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(6331..6597)
                     /locus_tag="VYR08_000446"
     CDS             complement(6331..6597)
                     /locus_tag="VYR08_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV3466022.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6862..7248)
                     /gene="rplQ"
                     /locus_tag="VYR08_000447"
     CDS             complement(6862..7248)
                     /gene="rplQ"
                     /locus_tag="VYR08_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV3466023.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(7260..8195)
                     /locus_tag="VYR08_000448"
     CDS             complement(7260..8195)
                     /locus_tag="VYR08_000448"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV3466024.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(8238..8621)
                     /gene="rpsK"
                     /locus_tag="VYR08_000449"
     CDS             complement(8238..8621)
                     /gene="rpsK"
                     /locus_tag="VYR08_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV3466025.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(8639..9004)
                     /gene="rpsM"
                     /locus_tag="VYR08_000450"
     CDS             complement(8639..9004)
                     /gene="rpsM"
                     /locus_tag="VYR08_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV3466026.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(9022..9138)
                     /gene="rpmJ"
                     /locus_tag="VYR08_000451"
     CDS             complement(9022..9138)
                     /gene="rpmJ"
                     /locus_tag="VYR08_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV3466027.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(9163..9381)
                     /gene="infA"
                     /locus_tag="VYR08_000452"
     CDS             complement(9163..9381)
                     /gene="infA"
                     /locus_tag="VYR08_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV3466028.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(9498..10136)
                     /locus_tag="VYR08_000453"
     CDS             complement(9498..10136)
                     /locus_tag="VYR08_000453"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV3466029.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(10287..11597)
                     /gene="secY"
                     /locus_tag="VYR08_000454"
     CDS             complement(10287..11597)
                     /gene="secY"
                     /locus_tag="VYR08_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV3466030.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(11610..12050)
                     /gene="rplO"
                     /locus_tag="VYR08_000455"
     CDS             complement(11610..12050)
                     /gene="rplO"
                     /locus_tag="VYR08_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV3466031.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(12195..12419)
                     /gene="rpmD"
                     /locus_tag="VYR08_000456"
     CDS             complement(12195..12419)
                     /gene="rpmD"
                     /locus_tag="VYR08_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV3466032.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(12391..12885)
                     /gene="rpsE"
                     /locus_tag="VYR08_000457"
     CDS             complement(12391..12885)
                     /gene="rpsE"
                     /locus_tag="VYR08_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV3466033.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(12903..13259)
                     /gene="rplR"
                     /locus_tag="VYR08_000458"
     CDS             complement(12903..13259)
                     /gene="rplR"
                     /locus_tag="VYR08_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV3466034.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(13343..13879)
                     /gene="rplF"
                     /locus_tag="VYR08_000459"
     CDS             complement(13343..13879)
                     /gene="rplF"
                     /locus_tag="VYR08_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV3466035.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(14071..14469)
                     /gene="rpsH"
                     /locus_tag="VYR08_000460"
     CDS             complement(14071..14469)
                     /gene="rpsH"
                     /locus_tag="VYR08_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV3466036.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(14754..15023)
                     /gene="rpsN"
                     /locus_tag="VYR08_000461"
     CDS             complement(14754..15023)
                     /gene="rpsN"
                     /locus_tag="VYR08_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV3466037.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(15041..15583)
                     /gene="rplE"
                     /locus_tag="VYR08_000462"
     CDS             complement(15041..15583)
                     /gene="rplE"
                     /locus_tag="VYR08_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV3466038.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(15607..15912)
                     /gene="rplX"
                     /locus_tag="VYR08_000463"
     CDS             complement(15607..15912)
                     /gene="rplX"
                     /locus_tag="VYR08_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV3466039.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIVEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(15990..16358)
                     /gene="rplN"
                     /locus_tag="VYR08_000464"
     CDS             complement(15990..16358)
                     /gene="rplN"
                     /locus_tag="VYR08_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV3466040.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(16384..16644)
                     /gene="rpsQ"
                     /locus_tag="VYR08_000465"
     CDS             complement(16384..16644)
                     /gene="rpsQ"
                     /locus_tag="VYR08_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV3466041.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(16669..16875)
                     /locus_tag="VYR08_000466"
     CDS             complement(16669..16875)
                     /locus_tag="VYR08_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV3466042.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(16885..17298)
                     /gene="rplP"
                     /locus_tag="VYR08_000467"
     CDS             complement(16885..17298)
                     /gene="rplP"
                     /locus_tag="VYR08_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV3466043.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(17302..17955)
                     /gene="rpsC"
                     /locus_tag="VYR08_000468"
     CDS             complement(17302..17955)
                     /gene="rpsC"
                     /locus_tag="VYR08_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV3466044.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(17968..18312)
                     /gene="rplV"
                     /locus_tag="VYR08_000469"
     CDS             complement(17968..18312)
                     /gene="rplV"
                     /locus_tag="VYR08_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV3466045.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(18324..18605)
                     /gene="rpsS"
                     /locus_tag="VYR08_000470"
     CDS             complement(18324..18605)
                     /gene="rpsS"
                     /locus_tag="VYR08_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV3466046.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(18709..19542)
                     /gene="rplB"
                     /locus_tag="VYR08_000471"
     CDS             complement(18709..19542)
                     /gene="rplB"
                     /locus_tag="VYR08_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV3466047.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(19560..19856)
                     /locus_tag="VYR08_000472"
     CDS             complement(19560..19856)
                     /locus_tag="VYR08_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV3466048.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(19856..20479)
                     /gene="rplD"
                     /locus_tag="VYR08_000473"
     CDS             complement(19856..20479)
                     /gene="rplD"
                     /locus_tag="VYR08_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV3466049.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(20504..21130)
                     /gene="rplC"
                     /locus_tag="VYR08_000474"
     CDS             complement(20504..21130)
                     /gene="rplC"
                     /locus_tag="VYR08_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV3466050.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(21347..21655)
                     /gene="rpsJ"
                     /locus_tag="VYR08_000475"
     CDS             complement(21347..21655)
                     /gene="rpsJ"
                     /locus_tag="VYR08_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV3466051.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(21922..22539)
                     /locus_tag="VYR08_000476"
     CDS             complement(21922..22539)
                     /locus_tag="VYR08_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV3466052.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(22536..23126)
                     /gene="nrdG"
                     /locus_tag="VYR08_000477"
     CDS             complement(22536..23126)
                     /gene="nrdG"
                     /locus_tag="VYR08_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV3466053.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(23119..23577)
                     /locus_tag="VYR08_000478"
     CDS             complement(23119..23577)
                     /locus_tag="VYR08_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466054.1"
                     /translation="MMTEFEKSQSAHDGGFWDTENFVYEEWLETNIQKEMGINLPENR
                     VPSIQFVLFGESGCALRFLNLRLRLNERLLNYAGHIGYSIRPSEKGKGYAKETLRQGL
                     QVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVERYWIEVANE"
     gene            complement(23670..23810)
                     /locus_tag="VYR08_000479"
     CDS             complement(23670..23810)
                     /locus_tag="VYR08_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466055.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(23822..26029)
                     /gene="nrdD"
                     /locus_tag="VYR08_000480"
     CDS             complement(23822..26029)
                     /gene="nrdD"
                     /locus_tag="VYR08_000480"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV3466056.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLDDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
BASE COUNT         7339 a         5769 c         4833 g         8111 t
ORIGIN      
        1 atagattcaa tacactatag aatagaattg taggttttaa aaacgcttac attgttttaa
       61 aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga tgtattatgc
      121 tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag acggtggttc
      181 gctagtcgtt tattgaccaa actcagaagg cttaattgga gcaactattc ctgcctttga
      241 agaaaaatat ggtatcaaaa tagaactgat tcaagctggt actggagaac ttttcaaaaa
      301 actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt cttatacaca
      361 atatgctacc cacggagaac tctttgaaaa ctatatttca aaagaaaatg ataatgttat
      421 caaagaatat caaaacacaa ctggcttctc tactccttat acactagatg gtaatgtttt
      481 aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa ggatatagcg accttctcaa
      541 acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta gcgcctttgc
      601 tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg cttggtctta
      661 tgtaaaagat cttttcacac ttattgatgg tatagtaaaa tgaaataaga acaggacaaa
      721 ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt gtactattct
      781 agtttcaata tactataaaa tcggttcaag ttcatctggt gtctataaag cagtcgctga
      841 tggagaaatg actgttggtc tctcttatga agatccagca gttaaactct taaatgacgg
      901 agctaacatt aaggtagtct atccaaaaga aggaaccgtc ttcctacctt ctagtgctgc
      961 tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa tttatcgatt ttattatctc
     1021 tcaagaagta caagatacac ttggtacaac cactactaac cgtcctgttc gtaaaaatgc
     1081 taaaacaagc gaaaacatga aaccaattga caaaatcaaa acactcactt aagattatga
     1141 ttatgtcatc aagaataaat cagatatcgt taagaaatac aacgaagtct ttacagatat
     1201 ccaatctaaa cagtaaaaga ggttcactat gagtgagatc gaaattatta acgccaaaaa
     1261 aatctaccac gatgtccctg ttattgagaa tttgaacatt acaattccaa aaggaagtct
     1321 ctttaccctt cttggagctt caggatgtgg gaaaacgacc cttcttcgta tgattgcagg
     1381 tttcaacagt atcaaagatg gagaatttta cttcgatgat acaaaaatca ataatatgga
     1441 acccagcaaa cgcaatatcg ggctggtttt ccaaaactac gctattttcc cacatttgac
     1501 tgtccgagac aacgttgctt ttggtcttat gcaaaagaag gttccaaaag aagaattgat
     1561 tcaacagacc aacaagtatc ttgaactcat gcaaattgct caatatgcgg atcgaaagcc
     1621 cgataaactc agtggtggac aacaacaacg tgtcaccttg gcatgcgcct tagcggttaa
     1681 tccaagtgtt ctcctcatgg acgagccact tagtaatctg gaggccaaac ttcgcttgga
     1741 tatgcgtcaa accatccgag aaatccaaca cgaagtggga attacaactg tttatgtaac
     1801 ccacgaccaa gaagaagcca tggctatttc agaccaaatt gctgttatga aagatggggt
     1861 gatccaacaa atcggccgac caaaagaact ctatcataaa ccagctaatg agtttgtggc
     1921 aacctttatc ggacgcacaa atattatccc tgccaatctt gaaaaacgga gcgacggcgc
     1981 ttatatcgtc ttttcagatg gctatgccct tcgaatgcta gctcttgatc aggttgagga
     2041 gcaagctatt catgtaagca ttcgtcccga agagtttatc aaagatgaat ctggagatat
     2101 cgaaggaact attagcgata gcgtctatct tggactaaat acggagtatt tcattgagac
     2161 aggttttgcc tcaaaaattc aagttagtga agaatcaact tttgaagaag atctacaaaa
     2221 aggcaatcgt attcgtctac gaatcaatac gcaaaaatta aacatctttt ctgcagatgg
     2281 ttcccaaaac ctgataaaag gagtcaacca tggaatgtaa aaaactaaat atttggacag
     2341 cctcctcttt cttcctcttt cttacctatc ttgtctttct cgtttatcct atcgttaccg
     2401 tgctcaagca agcacttata catgaaggac aattctcact agctaatttt gtcactttct
     2461 ttagtaaagc ctactactct gagacactag tcaacagttt cagggtttcc attaccgcta
     2521 ctgtcacttc cttagttgta ggaaccctat tagcttatct cttctctatg tatgacttca
     2581 aggggaagaa atttctacaa atattgatta tcattgcttc catgtcagct cctttcgtag
     2641 gagcctactc ctggattctc ttgctgggac gaaatgaggt catcactaaa tttttgacaa
     2701 atgcccttta tcttccagct atcgatattt atggattcaa aggaattgta cttgtcttta
     2761 cactgcaact attcccactg gtatttctat atgttgctgg aacaatgaac agtattgaca
     2821 attctctact tgaagcagct gaaagcatgg ggtccttcgg atttaaacct atcgtaacgg
     2881 ttgttttacc tctcctagtt ccaaccttac tagcagctcc ttgcttgtat ttatgagagc
     2941 attctcagac tttggaacgc ctatgttgat tggcgaaggg tatcggactt tccctgtcct
     3001 gatttatacc caatttatta gcgaggttgg aggaaattct gcttttgcaa ttatggcgat
     3061 tatcattgcc ttggcaattt tccttatcca aaaacacatt gcaaaccgct acagtttcag
     3121 catgaatctg ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa tggctgccat
     3181 ttatgcaaca gtctacggaa ttatctttat ctctgtttta cctcaaatct acttaattta
     3241 tacctctttc ctaaaaacat caggtatggt atttgttaaa ggttattctc caaacagtta
     3301 caagctagct ttcaatcgta tgggatctgc tattttcaat accattcgta tccctttgat
     3361 tgccttagtt ctagttgttc tatttacgac atttatctcc tacctagccg ttagaaaacg
     3421 gaatttgttt acaaacttaa ttgacagcct cagtatggta ccttatattg taccaggaac
     3481 cgttctaggg attgccttca tttctctggt ctatttggaa gtggatttct tatgattaca
     3541 gggactgctt tcatcttgat tatgtctcta tctgccagaa aattacctta tactattcgc
     3601 tcatctgttg ctagcttaca acaaatagca ccaagtattg aagaagctgc tgaaagctta
     3661 ggaagtagtc gtctcaatat ctttgctaag attacaactc caatgatgct atcggatatt
     3721 atttctggag ccatcctatc ttgggtcaca ctgatttcag aactctctac ttctatcctc
     3781 ctctacaatg tcaaaacaag aacaatgact gtagctattt atacagaggt tctcagagga
     3841 aattacggtg tagctgcagc cttgtcaact atcctgactg ttctaacagt aggttccttg
     3901 ctcttgttta tgaaaatctc taaaagcaat agcattacac tttagttttc cacatcaaaa
     3961 acagccgaaa ggagtaccct cggctgtttt ttcttatctt gatattctta tcaaatccct
     4021 agctcatggc aagcaagtct agactgatta aatagagcct tcttccatct tctcacgtcc
     4081 tagctctcgg taactacggt caccgacaac ttcaacccta aactgaccgt cctcatattc
     4141 aagaatcgtc acgctaccat tatctagacc atgcggatgc atgccattaa tcagataaac
     4201 aatggttcca atagtcattc catggctgac aacaagggcg ttacctccac cttgatcttc
     4261 catttctttt gcaatcattt caaagccttc cttgattcgg ccactgagtt tttcccagcc
     4321 ttcagcccaa ccagctgtat cgacctctac caagccctca gccagttcag cataagacaa
     4381 ttggtgaacg tggtccacat taaagatacg aggaataatg cccatgaaaa gatcgccatc
     4441 ataggctcca tcaaaactac cgaaacacca ttctctgata cgcttgtcca tgcgataagg
     4501 gattttcccc tgcaagccaa gttcttcaag gataattccc atggtctgaa tggtacgacc
     4561 agaatcactc gaataagcgc gctcaaactg tagatcagat tctcgcaaac cgattcctaa
     4621 ctcttgaatc cctcgttcac cttcagcagt taagggagta tcgctccaac cttgcgcgcg
     4681 accaatcgtg ttaaacatgg tcttgccatg acgtaccaaa tacaatcgta cttttaccat
     4741 tttccgtcct ccgtttgctt ctattatatc atgaatgata aaataaaagc cacccataca
     4801 ggcgactttt gaaggagatt attatgaaaa agtttaggag ttcattaaat aaagatatta
     4861 gatgaaaatc aaattcaaac taattcagtg ttatctgttt caaataatat taggaggtct
     4921 ttatttaatg attatagtat acacatctaa tcttaaatag aacttaaaat ttctcctatt
     4981 ttcttaattt ttaaaactat gaattggtgc tgggatttgt ccaccgcgag agatgaagtc
     5041 gacagacgaa gccccattaa ccctcataac gggtgcagtt cctaatagac caccaaactc
     5101 aatcatatcg ccttcttttc ctttgggaat gatacgaaca gctgttgttt tcatgttgat
     5161 aacaccgatt gctgcttcat ccgcaatcat agccgcaata gtttcagcag gcgtatcttc
     5221 tgggatggca atcatatcca atccaacaga acagatagcc gtcatagctt ctagtttttc
     5281 taaattaaga gagccatttt gcactgcagc aatcattcct tcatcctcag aaacagggat
     5341 aaaggcacca gataaaccac cgacctggtt acaggccatc actccaccct ttttaacttg
     5401 gtcgttcaag agggccaagg cagccgtcgt tccatgcgtg ccaactgttt ctagccccat
     5461 ttcctcaagg acacgtgcca cagagtctcc aaccgcaggg gttggtgcca aactcaagtc
     5521 cacaatacca aactccacac ccagtctctc actggccatt tgaccaacca attgaccgat
     5581 acgagtgatt ttaaaggcag ttttcttaac tgtttcggct actacatcaa agctctgtcc
     5641 acgaactttt tccaaagcac gtttcacaac accaggacca gaaactccga cattgatgat
     5701 aacatctgct tccccaacac catgaaaggc acccgccata aatggattgt cctcaacagc
     5761 attagcgaat acaaccaact tggccactcc catatctgaa agatttgctg tttccttgat
     5821 aattcgtccc atatctgcca cagccgtcat attaatacca gacttggttg agccgatatt
     5881 gactgacgag cagaccttat ccgtctcagc caaagcgcga ggaatggaat tgatgagaat
     5941 ctcatctccc ttttgataac ctttttgtac taaggcagaa aaaccaccaa taaagtccac
     6001 accaatctct ttcgcagcct tatcaagcgc ttttgccaga accacgtagt ccgtcgcatc
     6061 tgtcgctgcc ccaatcagag aaataggtgt caccgataca cgcttattaa cgataggaat
     6121 tcccaactca gccgcaattt catcaccaac agctactaaa ttagccgcct ttgtcgtaat
     6181 tttttgatag attttctccg cagcacgatt gatatctgga tcgatacagt ccaaaagaga
     6241 aatccccatg gtaatggttc tgatatcgaa attttgctcc tcaatcatgg cgatggtttc
     6301 agtaacttgt ctaatatcca tgataacctc ctagatatta tacatagctt cgaaaatcgc
     6361 tgcactctga atattgattt ttacattcaa agtttgccca aaagcttcaa attcattacg
     6421 aagataggta aaatcttgct tttcatcact agatacaata gccatcatcg taaaatattc
     6481 atccaagaca gtttgagaga tatcgtcaat attcaatcct aattctgcaa ttttaccaga
     6541 aacacctgca acaattccag atttatcttt accaacaaca gttataatcg ctttcataag
     6601 caaactccaa ttcttcttaa aataagaaac cttaacatta aacaggattc ttttcaaata
     6661 gtatagcata attctaacga aaatactcaa aaacgcctgc ttacgaagtt gttcttaaga
     6721 aaaaaacaat ttcgtaaaca agcgtctact atcccaactt atgatgagtg ttcccgctag
     6781 gaccgaaatc ctagcggtag accagagcta gactaagagc acaagactcc atcatcataa
     6841 cactcaacaa aattgatgat tttatactaa ttcgatgatc gccattggcg ctgcatcacc
     6901 acgacgtggt tcagttttaa ggatacgagt gtatccaccg ttacgttcag cataacgagg
     6961 tgcgatttct gagaacaatt tttgaagtgc tgtagtagaa gtgtacttat cagttgcttc
     7021 atcatagttt tcagatgcga tttcattacg tacgaaagca gctgcttgac gacgtgcatg
     7081 caaatcacca cgtttaccta gagtaatcat tttttcaaca gttttacgga tttctttagc
     7141 acgagcttca gttgtcacga ttgattcgtt gatcaaaagg tcagttgtca aatcgcgaag
     7201 cattgcttta cgttgtgagc tagtgcgtcc tagtttacgg taagccatgt attcctcctt
     7261 tatttatctt ttaatccaag acccaaatca atgagtttga gtttcacttc ttccaaactc
     7321 ttgcgtccaa gatttcgtac tttcatcatc tctgcttcag atttttctgt caaatcatgc
     7381 acagtattga taccggcacg ttttaaacag ttgtatgaac gcacagacaa gtccagttcc
     7441 tcaatcgtac gatctaaaat acggtcgtca gattcagtat cagcttcttt catcacttca
     7501 gttgacttag caatctcagt aagatttgta aacaaatcaa gatgttctgt caaaatacgt
     7561 gctgaaagcc ctaaagcatc ttctggaata attgttccat ttgtcaagat ttcaagggtt
     7621 aatttgtcga aaccatcatt gctacctaca cgagcaggtt ccacttgata gttgactttt
     7681 gtaactggtg tataaataga atctacagca agtgttccaa ctggtgcatt atccttttta
     7741 ttttcatcag caggtacata tccacgacca ctgttaacag tcatagtcgc ttttagagaa
     7801 gaaccttcac cgattgtaaa gagataatga tctggattta caatttcaat atcgctatct
     7861 gtcaaaatgt caccagctgt tacttcagca ggaccttcaa catccagttc gatgattttt
     7921 tcgtcttcaa cgtacgattt cactgcaatt cctttaatgt tcagaatgat ttgcatcacg
     7981 tcttcacgaa cacctggaac tgtgtcaaac tcatgtaaca caccatcaat gttgatagat
     8041 gtcacagctg ctcctggtag agaagctaga agtacacgac gaagagagtt accaagagtt
     8101 gtaccgtagc cacgttcaag tggttcgatt acaaacttgc cataatcttt attttcatca
     8161 atttttgtta tatttggttt ttcaaactcg atcatttagt tactccctct taaacgaaaa
     8221 gcagtgtaat gcgatgatta tacacggcga cgttttggag gacgagcacc attgtgtggc
     8281 actggagtca catcacgaat tgctgttact tcaagaccag cggcagcaag cgcacgaata
     8341 gctgactcac gaccagaacc tggacctttt acagtaactt caactgattt aagaccgtgt
     8401 tcttgtgcag atttagcagc agcttcagaa gccatttgag cagcgaatgg tgtagattta
     8461 cgagaacctt tgaaaccaag agcaccagct gatgaccaag caattgcatt accatgcaca
     8521 tcagtaatca taacaatagt gttattaaat gtagcgtgaa tatgagcaat accagattcg
     8581 atattctttt tcacacgacg tttacgtgtt ggtttagcca agacttttac ctcctatatt
     8641 attttttctt accagcaatc gcaacagctt tacctttacg agtgcgagcg ttgtttttag
     8701 tgttttgtcc acggacagga agtccacgac ggtgacggat accacggtat gaaccgattt
     8761 ccatcaaacg tttgatgttc aagtttactt cacgacgaag gtcaccttca actttgattg
     8821 catccacttc acgacggata gcatcttctt gatctgatgt aagatcacgt acacgaacat
     8881 cttctgagat tccagcagca gccaaaattt tcttagatgt tgcaagtccg ataccataaa
     8941 cataagtcaa tgagattact acgcgtttgt catttggaat atcaactcca gcaatacgag
     9001 ccatgttttc tcctttctat cttatccttg acgttgtttg tgttttggat ttgctgggca
     9061 aattaccata acacgaccat tacgacgaat aactttacag tattcgcaaa ttggtttgac
     9121 cgatggtctt actttcattt cttatccctc caagtttttc gattatttaa agcggtaagt
     9181 gatacgtcca cgtgtcaagt catatggact catttcgaca gtaacacgat ctcccgctaa
     9241 aatacgaata tagtttttac gaattttacc agaaactgtt gctaaaatct gatgtccatt
     9301 ttcaagttca accgtaaaca ttgcattcgg cattgtatca actactttgc cttcaacttc
     9361 aatcacatcg tcttttgcca cgcaaaagca cctccataaa tttcgattcg atgcctctag
     9421 acacagagac aacaattata agtcagacta tctcagtata acatttgtag cggattttgg
     9481 caagtgtgaa aaacgcttta tttcaaattt gtcaatactt tttcaatatc tgagaagaca
     9541 tcattgatat cttgattacc ttcgatgtca tgaaccaaac ctttggcacg gtagtgagca
     9601 atgattggtt ctccttgagc aatattaaca tccaaacgac gttttactgt ctcaggctta
     9661 tcatcttcac gttggtagta atcttcttct ttatagtcaa ctggtgggtt aaagaccttg
     9721 tggaaagttt ctccagttac gcggtggatg atacggccac tcaaacgttc caaaaggctg
     9781 ttagggttca cttcaatatt gataacacct tctagttcaa tgccaagttc agccaatgtt
     9841 ttgtccaagg cctgagcttg ttcaattgta cgtgggtaac catccaataa gaatcctgtt
     9901 tctttaatat catcttgtga aagacgttct tttacgattc catttgtaac ttcgtcagga
     9961 accaattcac ccttgtcaat atatgactta gcaagaacac ccatttcagt ttgatttgcc
    10021 attgcagcgc ggaacatatc acctgttgag atatgtgcaa catggaattg ttctacgatt
    10081 tttgctgctt gagttccctt acctgcacca ggtaagccca taatcaaaag attcatgatt
    10141 tgatctcctt attttatttt taaatagaag caaaaagtct tttcacccct atttaaaaac
    10201 aaaatagaag aggggagatc caactcttac aaatgtcaga gtttaaacct ccactccctc
    10261 agtatttact gattcagtaa atacttttat tctgttctgt ccatgaaacc aacatactta
    10321 cgtttcaata ggtaaccttc caattgcttg attccttcga tacctgtaga gataatgatc
    10381 aagagacttg ttccaccaaa ggcaacaaca tcagaaagac caaatacatc tttagctgca
    10441 atcggtaaaa tggaaatcac accaaggaag agggaaccaa cagttgcaag acgacgaaga
    10501 agtttagaca tatattcttc tgtaccttta ccaggacgaa ctccatggat ataggcacca
    10561 ctcttttgta ggctctctgc tgctttttca ggattaatct gtacaaacgt atagaagaat
    10621 gtaaagagaa taatcaacaa agcatacatg gcaataccag ttggagaagt agttgccaac
    10681 atctcttgtg ctacccttac ccaagcccaa tcatgacctg tggcactcaa aaactgaaga
    10741 atagccgcag gcgctgcagt aatcgaactg gcaaagataa cagggataac tccagcagga
    10801 tttaccttta acggaaggta agagctagat ggagcacctt gtgcaacctt agtatattgg
    10861 attggaattt tgtattctgc ttgttgaaca taagttgtaa agtaaataat caacaataca
    10921 gtaataatca aaatgattac gaaaatgata gatgaagtga tacggctact tgggacgttc
    10981 acaaagtagt ccacatagat gccctgaatc atctctggaa ttgaggaaac aatcccggca
    11041 aagataatca tggaaacacc gtttccgtat cccttatctg taatttgctc acccaaccaa
    11101 gtgacaatca tactaccagc tgttaagatg ataccaatcg tcagaaaaac ttgtggagtt
    11161 aaagcagttt taatcaattg agctccagcc aaggtattaa aaccagctgt aatcccgata
    11221 gattgcacaa aagcgagaac tagagcaata taacgagtag cttgattcaa ttttcttcga
    11281 cctacttccc cttgtttacc ccactctaca aacttgggta aaatatccat ttgcaagagt
    11341 tggacaacaa tagaagcggt gatataggga ctaactccta gggcaaaaat cgaaaagttt
    11401 tttagggcat tccccgacac caagctcaac atgtttaaga aggataatcc acttaaagca
    11461 ttcaagctat tggcattcac accaggaact gtaatgctag ttccgatacg aaagaccaaa
    11521 acgataaaaa ttgtaaataa aatttttgat cgaacctgct tgactttaag agcttctctt
    11581 aataatttaa aaaacatagg tcacctctct tagatgactt ctactgaacc acctttagca
    11641 gtgatagctt cttcagctga tttagagaat ttagctgctt tcacagtcaa tttcttagtc
    11701 aactcaccgt taccaagaat tttaatacct gacttttcag ctttaacaat tcctgcttcg
    11761 ataagaacaa ctggagttac ttcagcacca tcttcaaaga cgttcaattg gtcaaggttc
    11821 acaattgcgt attctttagc gttgatgtta gtgaatccac gttttggaag acgacggaac
    11881 aatggagttt gtccaccttc aaaaccaagg cgaactccgc caccgctacg agctttttga
    11941 cctttttgac cacgaccaga tgttttaccg ttacctgatg aagtaccacg accaacgcgg
    12001 ttacgtactt tacgagaacc ttctgcaggt ttcaattcat gaagtttcat ttttattttc
    12061 tcctcttttg taaaatgcta gcgccgataa gggagaaaag gttgtctccc ccatcaactc
    12121 gcctatacga tgtcatcata gatgactata tctagtttta ggggatggta cagtgcacat
    12181 cccctaaaac ttcattagtt tacttcttca actgttacta agtgagatac tgctgtgatc
    12241 ataccacgga tagcagcgtt atcttcttta ataacagagc tgttcaattt gccaagtcca
    12301 agtgctacaa cagttttacg ttgtgatgga atgcgtccga ttggagactt agtcaaagta
    12361 attttaattt gagccatttt atcccctttc ttatgccaaa tcagaaactg aaataccacg
    12421 aagggcagca atttcttcag cgcgtttcaa ttgtttcaaa ccttcaacag ttgcacgaac
    12481 aatgttgatt ggagtgttag aaccaagtga tttagatgta atatctgcca cacctgccaa
    12541 ttccacaacg gcacgaactg caccaccagc ggcaactcca gaaccttcta cagcaggttt
    12601 caacaatact ttagctccac cgaattctga aagaacttcg tgtgggattg ttgttccaac
    12661 cataggaact tcgatcaagt ttttcttagc atcatctact gctttacgga ttgcttctgg
    12721 aacttcttga gctttaccag taccaaatcc tacgcgacca ttgtggtcac caacaacaac
    12781 aagagctgcg aaacgaagac gacgtccacc tttaacaact tttgtaacac ggttgacagc
    12841 aactacgcgt tcttctaatt caactgcatt gtctttaaat gccattttct agtgtcctcc
    12901 tattagaatt tcaatccgtt ttcacgagct gcatcagcca aagctttcac acgtccgtga
    12961 tatagatatc caccgcggtc gaacaccact tctgaaatac ctttagcgtt tgcacgttct
    13021 gcaacgagtt taccgacagc aacggcttgt tcagttttag ttccttttga aacttcttta
    13081 tcaagagttg aagcacttgc gagcgttaca cccgctacgt catcaatcac ttgagcgtag
    13141 atgcctgtat tagaacggaa tacgttcaaa cgtgggcgat cagcagttcc agagagtttt
    13201 ccgcgaacgc gacggtggcg tttttggcgg agtttgtttt tatctggttt tgaaatcaca
    13261 gttttcacct ctttagtttt aaatcgtgtg ctatgcacaa agttggaaaa taggttggtg
    13321 gttgatgatc aaccactcaa cattatttac ctgttttacc ttctttacgg cgaacgaatt
    13381 caccaacgta acggatacct ttacctttat atggttctgg tgaacgaagg ctacgtacgt
    13441 aagcagctgt ttgaccaact acttcttttg aaattccgct aacaacgatt gttgttgggt
    13501 ttggaagttc aaaagtaatt ccttctggag cttcaacttc gtctggatga gatttaccaa
    13561 cagccaaaac aagtttagat ccttgaagct gtgcacggta accaacccca cgcatttcaa
    13621 gttctttctt gaatccttct gatacaccaa caaccatgtt gttcaaaagg gcacgagtag
    13681 ttccgtggat agttttcatt tcttttgaat cgtttggacg gtgaagagtt atttcagtac
    13741 cttccacacg gatttcaata tcttttgaga actcacgagt aagttctcct ttaggtcctt
    13801 ttacagttac aacgttgtca ttgttagcga gttcaacacc agcaggcaac acgataactt
    13861 tattaccaat acgtgacatg tttattttct cctgttaaat tgtcaggcca gaacggccag
    13921 ttttcacggg gttcagatac ttatttagtt caagagctga actgaacacg ctctaagaac
    13981 tgtgcaaaat agataagttg gcttgtttgt aaacaaactt ctccaacttc ctaattttgc
    14041 tttgttcttt acgagctttg tatcttgatt ttaccaaacg taagcgataa cctcaccacc
    14101 aacattcttt tggcgtgctt ctttatcagt aagcaaacct tcagaagttg aaaggatggc
    14161 aattccaagt ccgttaagaa cttttggaag gtcttcacgt tttttgtaga cacgaagtcc
    14221 tggtttagaa acacgtttca agttagtgat aactttctca ccatttggtc cgtatttaag
    14281 aaatacacgg atgacgcctt gtttgtcatc ttcaatgatt tcaacgtttt ttacaaaacc
    14341 ttcgcgttta aggatttcag caatcccttt tttgatgttt gatgcaggta cttcaagtac
    14401 ttcgtgttta gcttggttag cattacgaat acgagttagg aagtctgcga ttgggtcagt
    14461 cataaccatt ttgtttttct cctcttacta gtagtttgca agttgcactt gctagttaat
    14521 acatgataca aagagcgtaa cagcacgagc aaaaatagac aatttaatgc agaagcaatg
    14581 ctccaaagca aattggtctt ttttgccaaa gctgtagctc gtgttcaaat tggcaagccc
    14641 aattgaaccc gggctaaact ctgtgtgaaa aagataaact ttcctagaaa ctttagtttc
    14701 ttcgtcaagt ttcctatttt tacttggagt tttgacgctc ttgatatctt aaattaccaa
    14761 gatgcttttg ttacaccagg aatttgacct ttatgcgcaa gttcgcgaaa agcgatacga
    14821 ctcagaccaa atttgcggta aactgaatgt gggcgccccg taactctaca acgattatgt
    14881 aaacgagtcg gtgaggcgtt gcgaggtaat ttagataaac cttcgtagtc ccctgccgcc
    14941 tttaatgcag cacgtttttc agcataacgg tcaacaattt tttggcgttt agcctctcta
    15001 gctaccattg attttttagc cattagattt acctcctata ttattttgca aaaggcattc
    15061 caaggcctgt aagcaatgca cgtgactctt cgtcagtgtt agcagttgtt acgataacga
    15121 tgtcaagacc acgagttttg tcaacgtcat cgaagttgat ttctgggaag attaattgtt
    15181 ctttcacacc aagtgtgtag ttcccgcgtc catcaaatga ttttgttgga acaccgtgga
    15241 agtcacgtac acgcggaagt gaaactgata ccaatttatc caagaattcg tacatacgtt
    15301 caccacgaag ggtaactttt gcaccgatcg caacaccttc acgaagacgg aagccggcga
    15361 ttgatttttt agctttagtg ataagtggtt tttgacctga gataagtgcc aattcttcag
    15421 cagctttttc aaggctttta gcgtttgata cagcttcacc aacacccatg ttcaaaacaa
    15481 tcttatctac tttaggcaca gccatcactg atgagtagtt gaattgttct gtcaaagcag
    15541 gaactacttc attaagatat ttttctttta aacgatttgc cattatactt ctcctttcct
    15601 tcgtgattaa tcaagcactt cgcctgattt tttgttgtag cgaacttttt taccgtctac
    15661 aaatttgtat ccaacacgac cagctacacc atttttgtcc aaaacttgaa cgtttgatac
    15721 gtggatagct gcttctttct cgacgatacc accttgagga agctcgttag ttggacgttg
    15781 gtgtttctta acaatgttaa caccttcaac gataactttg tttacttttg gaagggcagt
    15841 aaggacaaca gcttctgttc ccttatcttt accagcgatt acgcgaactt tgtcgccttt
    15901 ttttacaaac attaggtttc tccttgattt ttcttacgcc cataagggca ccctagcttg
    15961 aagctagggg actagtttgt tcctaaaaat taaagtactt ctggagcaag tgacacgatc
    16021 ttcatgaagc caccttcacg caattcacgt gcaactgggc caaagatacg tgttccgcga
    16081 ggagttttgt cttcacggat gataactgct gcgttttcgt caaatttgat gtatgaacca
    16141 tcagcacgac gagcacctga tttagtacga acgataactg ctttaacaac gtcacctttt
    16201 ttaaccgcac caccaggagt agcttgtttt acagatgcca cgataacatc accgatgttt
    16261 gcaaatttac gtcctgaacc accaagaact ttgatagtca agatttcgcg agcaccgctg
    16321 ttgtctgcga ctttcaaacg agtttctgtt tgaatcattt cagttttctc ctttcaggtt
    16381 tgattagatg atgaccgctt cttcaacaac ttctacaaga cggaaacgtt ttgtagctga
    16441 aagcgggcga gtttccatga tacgtacgat atcgccttct ttggcaacat tgttttcatc
    16501 atgagctttg tattttttag agtagttaat acgtttacca tagactgggt ggttacgttt
    16561 tgtttcaact acaactgtga ttgtcttgtc cattttgtca gatacaacac gtccaacaag
    16621 aactttacga ttattgcgtt ccattgaaat ttctccttcc ctagtctatt atttcgcttc
    16681 agattgaact gttttgatac gagcgatttg ttttttaact tctttcaagc gagctgtttg
    16741 ttccaattga ccagtagcag cttggaaacg aagttcaaac aattcttttt tcaattcgtt
    16801 ttcgcgcttc gcgagttctt cttgagaaag accacgaagt tctttaacaa attcttttac
    16861 ttcattaagt ttcatgcctt ctccttattc tgcttcacgt tttacgaatt tacatttaac
    16921 tggcaatttg tggctagcaa gtcgaagcgc ttcacgtgca atctcttcag atacaccagc
    16981 gatttcgaac atcactttac cacgtttaac tggtgctacc caaccttcag gtgccccttt
    17041 accagatccc atacgcacac cgatagcttt agcagtgtat gatttgtgtg ggaagatttt
    17101 aatccaaact ttaccaccac gtttcatgta acgagtcatg gcgatacgag cagcttcgat
    17161 ttggcggtta gtgatccagt ggctagttgt agcttgaaga ccgtattcac cgaatgctac
    17221 ttcttttcca ccttttgctt caccgcgcat ttttccacgg aattcacgac ggtgtttaac
    17281 acgtttaggt actaacattg gttatttacc tcctttagtg tttttacgag ctggaagaac
    17341 ttcaccacgg tagatccata ctttaacacc aagtttaccg tatgtagtat ctgcttcttc
    17401 ccaagcgtaa tcgatatctg cacgaagtgt gtgaagcgga acagttcctt cagagtatcc
    17461 ttcagcacgg gcgatatctg caccgttcaa acgacctgat acttgagttt tgattccttt
    17521 agctccagca cgcattgcac gttggattgc ttgtttttgt gcacgacgga aagcaacacg
    17581 ttgctccaat tgacgagcaa ttccttcacc tacaaggtga gcatccaaat caggttgttt
    17641 gatttcgatg atgttgatgt gtacttgttt tccagtcaat ttgttaagtt ttgcacggag
    17701 tgcatcaacg ttagcaccac ctttaccgat aaccatacct ggtttagcag tgtgaagtga
    17761 aacgttaact ttgtttactg cgcgttcgat ttcaatagtt gaaactgctg cgtcagcaag
    17821 ttctttttga acgaatttac ggattgcaag atcttcatga aggtaatccg cgtattcttt
    17881 ttcagcatac catttggcat cccaatcacg gatgatgccg acacgcatac caattggatg
    17941 tactttttga cccacgattt tacctcctta tttttctgca acagctacag tgatgtgagc
    18001 tgtacgtttg ttgattggtg aagctgaacc tttcgcacgt ggacggaaac gtttcatagt
    18061 tggtccttcg tttgcgaatg cttcagatac taccaagtta gctttatcca aaccaaagtt
    18121 gttttcagcg ttagctacag ctgagttcaa aactttcaag atgatttcag cagctttgtt
    18181 tggagtgaat gtcaagattg cgattgcatc ggctacgctt ttaccacgga tgttatcaag
    18241 aacaagacgt gatttacgag gtgaaacacg tactgtacga gccattgctt tagctgaagt
    18301 aatttctgcc atttatgttc tccttatttt ctacgtgttt tcttgtcgtc tgcagcgtga
    18361 cctttgtaag tacgagttgg tgcaaattca ccaagtttgt ggcctaccat gtcttcttgg
    18421 atgtaaacag gcacgtgttt acgtccgtca taaactgcaa tagtgtaacc aatgaaactt
    18481 gggaagatcg ttgaacgacg tgaccaagtt ttaataactt ttttcttttc gtcgttagct
    18541 tgagcttcaa cttttttcat caaatgctca tcgacgaaag gtcctttttt aagactgcgt
    18601 cccattttta tattttctcc tttaaatgtt gtaccacagc ggtttgcgct cctatggagc
    18661 actaccgagc tggcggattt actagttgct taagcgacta gtttaatatt atttctcgtt
    18721 gcgacgacga acgataagtt tgtcagattt cgctttcttg ttacgagttt taagaccaag
    18781 agcaggtttg ccccatggag tagatggtgc tttacgacca actggtgctt taccttcacc
    18841 accaccgtgt gggtgatcgt tagggttcat tacagaacca cgaactgttg ggcggatacc
    18901 tttccaacgg ctacgtcctg ctttaccaag gtttacaagt ccatgttgtt cgtttccgac
    18961 aacaccaact gtagcacggc aagttccaag aatcatacga acttcacctg attgaagacg
    19021 aacaagaacg tatttacctt cagaacccaa tacttgagca gatgcaccag cagcacgtac
    19081 caattcacca ccacgacctg gtttcaactc gatgttgtga atcaaagtac caactgggat
    19141 gttagcaagt ggaagagcgt ttccgacttt gatatctgct tctggacctg aaacgatacg
    19201 ttgacctact tcaagacctt ttggagcgat gatgtatgct ttcacaccgt cagtgtagtg
    19261 tacaagagcg atgtttgcag aacggtttgg atcgtactcg attgttttaa caactgcttc
    19321 aacgttatct ttattacgtt tgaagtcaac caaacggtag aaacgtttgt gtccaccacc
    19381 ttggtgacga actgtgatac gaccgttgtt gttacgacca gccttgctct tcaatgcaac
    19441 aagcaatgat ttttcaggag tgcttgttgt gatttcagcg aaatccaaag aagtcatatt
    19501 acggcgaccg tttgttgttg gtttataaac acgaattccc acgatatttc ctccttagat
    19561 tattcagctt cagcagcaaa caactcgatt gctttagaat cagctgtaag tgtgatgata
    19621 gcttttttag ttttgttagt aaaaccagtg taacgtccaa cacgtttagc ttttggtttt
    19681 acgttgattg tgttaacatt ggcaacttta acaccttcga aagcagcttc aacagcttgc
    19741 ttgatcaaaa gtttgtgtgc acgagtgtca acttcaaata catatttccc tgcttcaagt
    19801 tgagccattg agctttcagt gatgacaggt tttttgataa catcatacaa attcattatg
    19861 caagaacctc ctcgatttta gagatagctg cttgtgtgac aagaagtttg tcgctatttg
    19921 cgatgtcaag aacacttgca gttgtagcag ttgcaacttt cacgtttgga aggttacgag
    19981 ctgaaagagc tgcgaattca tttccttctt caaggataac aagaacttta gaatcgatgc
    20041 tcaatgctgc aagaactttt gcaaattcag cagtttttgg agctgtaaat gaaagagcgt
    20101 ctacagctac gaatttgttt tcagcaactt tttcagagta aactgattta agagctaggc
    20161 gacgaacttt ttgtggaagt ttgtagccgt atgaacgtgg agttggtccg aagacaacac
    20221 caccaccacg ccattgtggt gagcggatag aaccttgacg agcacgtcca gttccttttt
    20281 gacgccatgg tttgcgtcca ccacctgata ctgcagagcg gtttttaaca gcgtgtgttc
    20341 cttgacgaag gcttgcgcgt tggctgatga ttacatcaaa cacaactgat tcatttggtt
    20401 cgataccaaa tactgcatcg ctaagaacaa cttggccagc ttctttacca gtttggtcaa
    20461 ataatgttac gtttgccatt gtgactgatt tcccctttct ttattattta ccagctttaa
    20521 ctgctgattt gatagtgata agagatttct tagcacctgg tacgttacct ttgataagga
    20581 taacgttctt ttctggaaca acttgtacaa cttcaaggtt ttgaattgtt acgcggtcgc
    20641 cacccatacg tcctgcaagg tttttacctt tgaatacgcg gttaggtgca acaggcccca
    20701 tagaacctgg acgacggtgg taacgagaac cgtgagccat tggtccacgt gattgtccgt
    20761 ggcgtttgat aacaccttgg aaacctttac ctttagaagt acccgttacg tcaacaacat
    20821 ctccagctgc gaatgtttca actgtgattt cagcgccaac ttccaagcct tcaacgtttt
    20881 tgaattcacg aatgaagcgc ttaggagccg tgttagcttt cgctacatgt cctttagcag
    20941 gtttgttgct caatacttcg cgtttgtcat cgaaaccaac ttggatagcg ttgtatccgt
    21001 ctgtttcaac agttttaact tgaagaacaa cgtttggagt tgcttcaata actgttacag
    21061 ggatcaattc gccagcttca gtgaagattt gagtcattcc cacttttttc cctaagattc
    21121 cttttgtcat gagaaaatag ttccttttct atatttttta ttcaaaaagt ttttaacgag
    21181 cgttttttat gctcaagtct aagatacaaa ggacgtcaaa ctcaacgtga aaataggaaa
    21241 ctgacgcagt gtctagcaga cactaggaag tttatctttt tcacaccgag tttagcccgt
    21301 gttcaattag attgaaacac cagcctctgc tcttttatat tttagattaa agtttgattt
    21361 ctacgtttac accacttgga agatccaatt tcatcaaggc atcaactgtt ttttgagttg
    21421 ggttaacgat atcgatcaaa cgtttgtgtg tacgcatttc aaattgttcg cgagagtctt
    21481 tgtatttgtg agtcgcacga atgattgtgt agaggctacg ttcagttgga agtgggattg
    21541 gacccgcaac ttgtgcacct gtacgagtag ctgattctac gatttttgca gccgctgtgt
    21601 caagcgtacg gtgttcgtaa gctttcaaac ggatacggat ttttttgttt gccatctttt
    21661 tctccttttc gtctatttaa gataataggc tagctccaca agaaaaccga cgcggtgttg
    21721 cgtggcaatg caaccgagcg tgtcgcaacc tcttgcatca aaactaaggc tgtaatttac
    21781 agcaccataa tagaataaca caaagcccct gcgattgcaa gggatttgtt gctgtttttt
    21841 cgtttttttg ggatgaaact gttttcctac tttttctttc taaaattagg gattttcttc
    21901 tatatttctt ctatacataa gttaagtcct cttgacatca aatttatctt ctgattggtc
    21961 cactaagata tccgccttag actcagtttc tttatagtat cgcagatact gctcccgtct
    22021 catctgatgg ctagccaata taaaggatgc atcgcgattt ctcacagtcg tatctctagc
    22081 aaggcgtcgc tttaattcgg tctcctcatc cgtgtagaaa cagatggttt tttcaaagag
    22141 ttccttgggt agaaagccaa cagacatccc ttcgacaatc aaaattggtt tggctccaga
    22201 caagacctca ctagccttcc aaggttcttc aattgtcaag acatccatac ccgcctgcaa
    22261 ggcaaggata tctctctgca aactctccag ttcatgcgcc actggcagac tggctgtcac
    22321 cttttgattc ggcgcgtcct tgggtactac cagatggcgt cctgaggtga tataaggatc
    22381 tgtctctagc aaatttactg tagtagaatc taaagcttgg tacaattcct gtgcaaaggt
    22441 tgatttacct gaagctccat gaccgtatat tcccagtgtc ctgactttcc ccgtctcgat
    22501 ctctgagact agttggtcta ctaagtcttt tttcttcatt ctctcttcac ctgttcatag
    22561 ctttcttttc cgtcattgag cttgtcccaa atcactactt gcccactttt gagcgatttt
    22621 tgcacatcga taattcgttg gttagacgaa cctcgaaact ggagcataag atttctctta
    22681 gttcgatcat atcttccatc gacaagaatg tcaatcagtg acaagagttc cagtttatct
    22741 ggagtttcca acatcatttc ttcccaagtg tagccggtcc aggaccaaat gtccttgtct
    22801 ggcaattcct ttcggatgcg tttaactaga ggcaagagaa tgccagtatt gagaaaaggc
    22861 tcccctccca gcaaagtcaa gccttgaaca tagggttggg caaggtctgc cataatctgc
    22921 tcttctaatt ctgctgtata gggaatgcca gcattaaaag accaagtcgc aacattataa
    22981 catccctcgc agtgaaacat acagcctgat acatagagag agttacgcac gccttcgcca
    23041 tcaacaaagt taaaggcctt gtagtcaatg atacgacctt gactaagttc ctcgcttttc
    23101 cattcttgtg gttttggatt attcattcgc tacctctatc caataacgct cgactccatt
    23161 gtgagcatcc tcaaatattc caccatttgc tagaatgact gctctgctag caggattatt
    23221 cacgctacag gtcaccagag ctttcttgat gttcttttcc ttagcaactt gcaagccctg
    23281 acggagagtc tcttttgcat aacccttgcc tttttcagat ggacgaatgg agtagccaat
    23341 gtggccagca taattcagta acctctcatt cagtctcaat cgcagattca aaaatcttag
    23401 agcacaacct gattcaccaa acaatacgaa ctgtatcgat ggaacgcgat tttcaggcaa
    23461 gtttattccc atctcttttt gaatatttgt ttccaaccac tcttcataca caaagttctc
    23521 tgtatcccaa aatcctccat catgggctga ttggctcttt tcaaactctg tcatcatatc
    23581 taaaacagct ttctttatct gctaatcttg gtctgcgtaa ttccatcctt acctcccact
    23641 atgagaaaag cgagagctgg ctctcacttt tattttttct tgttcttgtt taaaaattgt
    23701 tctctgaagc taagcaagcg ttctttctta ccagctccac ctttagagtg tttctcgtga
    23761 taacgggtca cttgtgcgcg ccccttatcg tctagttgat attttcccat ttcatttctt
    23821 tctaatttgt tacttgatgc ccagctattt taatcgttga accattcata tgtttgacac
    23881 gcgcagcgat ttccttgtga cgcccgttga ccatcggtct tgcttgagga ttacctaggt
    23941 agccacaagt tcgcttcacc acatctactg ttttagggtc gctattgcca cagtttggac
    24001 aagcaaaccc tctctcagtt ggttcaaaat ccccttcaaa gtcacacttg tagcaacggt
    24061 caatcggagt attggtgcct agatagccta cacggtcata agcataatcc cagacagctt
    24121 ccaaggcctt tggattttgc tgaaggactg gatactcaca ataatggatg aaaccacctg
    24181 acgcacctgc ttctggatag actttctcaa agtccaattt ttcaaacggt gttggatttt
    24241 tacgaacatc gtagtggaaa gagttggtgt agtattcctt gtctgtgata tcaggaatag
    24301 agccaaactt gtctatatct agtcggcaga aacggtctgt caaactttcg gatggtgttg
    24361 agtagataga gaaatggtag ccatattggt ctgaccactc ttctacacgg cgtttcatat
    24421 cgtgaatgat gtctagcgtg aattccttag catctggatt actttcccag ctgttaccaa
    24481 agaaaactgt cgctacttca tacaagccga tatagcccag cgaaacggtt gcccgacgat
    24541 tcttaaagag ctggtcaaca ctttcttctt tacctagacg atggccaaaa gcaccgtact
    24601 gataaagaat aggagcattc gctggtgtcg cctctttagt gcgttcgaca cggtaaacaa
    24661 gagcatcttc tgcgatattc attcgctcgt tgaagatttc ccagaactta ttcatatcac
    24721 cttcagactc aagagcaata cgaggcagat taaccgtcac aacacccaga ttcatgcgac
    24781 ctgaattgac ttctacacca ttttcatcct tccatccttg gaggaaagaa cggcaaccca
    24841 taggcacctt gaaggatcct gtcaagtcaa caatcttatc gtaagataaa acatctggat
    24901 acatccgttt ggttgcacac tcaagagcca actgcttgat gtcgtagttg ggagttcctt
    24961 cctctaaatt aaggcctctt ttcagagtga aaataagttt aggaaagata gccgtacggt
    25021 gttctgaacc aagacccttg atgcgaatgt ttaaaatagc tttttgaatt tctcgttcaa
    25081 aacgactggt tcccagacca aaacctagcg aagtaaaagg tgtttgtcca tttgaagtga
    25141 agagagtatt gatttcatac tcaagagatt gcatggcatc gtagatgtcc ttttgcgttt
    25201 tcttccaagc gtaatcttcc cgtttttcag gcaataccca ctcttctgca tctttgagat
    25261 gtttttgata gttcttctct gcataaggcg ccaaaatttc atcgatacgg tcagctgaac
    25321 agccaccgta ctggctagaa gcaacgttgg caatgatttg agaaatctgt gctgtcgcag
    25381 tttggataga cttgggactc tctacctctg catttccaat cttaaaacca ttttccaaca
    25441 tacccttaaa atcaatcaaa cagcagttgg tcataggggt atagggactg tagtccaaat
    25501 cgtgatagtg gatatccccc ttttggtggg cattggctac gtgcttagga agcatttgca
    25561 gtccgattga tttcccaaca atccctgctg tcaaatcacg ctgagtgtta aagacatcac
    25621 tgtctttatt agcattttca ttgacaactg tctggtcttt gttgagaagt ttatgaatac
    25681 taaagttgat atccgtcgct tttgagcgct caaaatccct ctgtgtccga taagtaatat
    25741 actcctcagc cagcgcatat tctttggctt caaggagttc atgttctacg atattttgaa
    25801 tttcgtaaat cttaattccc tgtggaaagc gactatgaat ttctgtaata attcgctcag
    25861 tcagatcatc gaggcgtttt tcaaccaggg gtgtcacatc cataaccttg tcagccgcct
    25921 tgtggagagc cttgtcaatc ttgtccacat caaatacaac acgtctccca tctcgtttct
    25981 cgacgaagag agttggcaaa attgtaattt tttcttctag tgcaatcata tgcatgctct
    26041 tttctaaaat gt
//
LOCUS       DAVMHG010000014        24511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVMHG010000014 DAVMHG010000000
VERSION     DAVMHG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.014"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(236..1084)
                     /locus_tag="VYR08_000481"
     CDS             complement(236..1084)
                     /locus_tag="VYR08_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3466057.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1236..1514
                     /locus_tag="VYR08_000482"
     CDS             1236..1514
                     /locus_tag="VYR08_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466058.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1516..3579
                     /locus_tag="VYR08_000483"
     CDS             1516..3579
                     /locus_tag="VYR08_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV3466059.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3657..4913)
                     /locus_tag="VYR08_000484"
     CDS             complement(3657..4913)
                     /locus_tag="VYR08_000484"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV3466060.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5055..7520
                     /locus_tag="VYR08_000485"
     CDS             5055..7520
                     /locus_tag="VYR08_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3466061.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEEKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7585..8496
                     /locus_tag="VYR08_000486"
     CDS             7585..8496
                     /locus_tag="VYR08_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3466062.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8726..9424
                     /gene="dapD"
                     /locus_tag="VYR08_000487"
     CDS             8726..9424
                     /gene="dapD"
                     /locus_tag="VYR08_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV3466063.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEINAQ
                     TQQKTALEDALRTL"
     gene            9492..10622
                     /locus_tag="VYR08_000488"
     CDS             9492..10622
                     /locus_tag="VYR08_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV3466064.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIESAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10634..11173
                     /locus_tag="VYR08_000489"
     CDS             10634..11173
                     /locus_tag="VYR08_000489"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV3466065.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11157..11834
                     /locus_tag="VYR08_000490"
     CDS             11157..11834
                     /locus_tag="VYR08_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV3466066.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(11901..12800)
                     /gene="galU"
                     /locus_tag="VYR08_000491"
     CDS             complement(11901..12800)
                     /gene="galU"
                     /locus_tag="VYR08_000491"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV3466067.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKIDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKEDLKNYLIQLGKELTEKE"
     gene            complement(12822..13838)
                     /locus_tag="VYR08_000492"
     CDS             complement(12822..13838)
                     /locus_tag="VYR08_000492"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3466068.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14050..14430
                     /locus_tag="VYR08_000493"
     CDS             14050..14430
                     /locus_tag="VYR08_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466069.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKEGVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14604..15029)
                     /locus_tag="VYR08_000494"
     CDS             complement(14604..15029)
                     /locus_tag="VYR08_000494"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3466070.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15097..15747)
                     /gene="phoU"
                     /locus_tag="VYR08_000495"
     CDS             complement(15097..15747)
                     /gene="phoU"
                     /locus_tag="VYR08_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV3466071.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15762..16514)
                     /gene="pstB"
                     /locus_tag="VYR08_000496"
     CDS             complement(15762..16514)
                     /gene="pstB"
                     /locus_tag="VYR08_000496"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV3466072.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16516..17331)
                     /gene="pstA"
                     /locus_tag="VYR08_000497"
     CDS             complement(16516..17331)
                     /gene="pstA"
                     /locus_tag="VYR08_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV3466073.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17324..18187)
                     /gene="pstC"
                     /locus_tag="VYR08_000498"
     CDS             complement(17324..18187)
                     /gene="pstC"
                     /locus_tag="VYR08_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV3466074.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNPPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18305..19180)
                     /locus_tag="VYR08_000499"
     CDS             complement(18305..19180)
                     /locus_tag="VYR08_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV3466075.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19347..20678)
                     /locus_tag="VYR08_000500"
     CDS             complement(19347..20678)
                     /locus_tag="VYR08_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV3466076.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20679..21386)
                     /locus_tag="VYR08_000501"
     CDS             complement(20679..21386)
                     /locus_tag="VYR08_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466077.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21470..21946)
                     /locus_tag="VYR08_000502"
     CDS             complement(21470..21946)
                     /locus_tag="VYR08_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV3466078.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            21953..22480
                     /locus_tag="VYR08_000503"
                     /pseudo
     CDS             21953..22480
                     /locus_tag="VYR08_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22795..24486)
                     /locus_tag="VYR08_000504"
     CDS             complement(22795..24486)
                     /locus_tag="VYR08_000504"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV3466079.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
BASE COUNT         7100 a         4700 c         5303 g         7408 t
ORIGIN      
        1 attctttgag acgggagaaa tcgaaatact tgatttatac ggcctatatc atatttgggg
       61 taaatgtagt atatggtatt caatggttat taagagaact gatttcaact atttcccctt
      121 aatgtgtatg gaaactataa caaaacgcat aagattaagg tttgtgtacc tgttcttatg
      181 cgtttttata atattgaaga ttttcggtaa gttttatacc tttgggaagt ctagtttaga
      241 atgctttccc aaccaaaatc tctgcttcga tggtaatctc tgtcagttgg ctccagtcaa
      301 tcttgctctg gtcaacgtga aagaggagag gagtcatact gagtagggct tggagttgtt
      361 ctgctgtgat agtcttagtc agagaggcag tttgactaga taggatggta aagtgttcct
      421 ggaaatgctc cttgatatct tggttagaat actccttgtt tgtcagctgg tcctgtactc
      481 tttgacggat ttctttgagg tgattttcag ttgggataac ctttatcaag ataccgtctt
      541 tggataaaac gcgacgaaat tctccatagt tggcaggtga gaagatatca agcagaatat
      601 ccatgttagc gtcttttata ggaagtcgtg ccaagtcgcc aacaaaccaa ttgactgccc
      661 agttgggttc acttttagca gcgatttgga ctgaatcttt ggagatgtca aaggcataga
      721 aagttttttc agagtgactt tcttgtagtt tgcgagaata gaatccttca ccacaaccga
      781 tatccaaaat tgttgtggta gtttttgagc ttgcaagcaa gtcagataca gcatctaaga
      841 tagcttggta aaagccggct tctaggattt gttgacggtt ttgaaaattt tccttgttat
      901 agttagcaga ttgcttgatt tgaggtgcta gattgacata gccaaatttc gccaagtcaa
      961 aagaatgacg gttgcagcac ttgaaattag tctctaacag agtcagattt tcttgacaga
     1021 taggacaggc aaaggcagtc gcagaagcaa aacgctgaag tttgggcttg agatttgtat
     1081 tcatagttta agtgtatcaa aagaggcaaa tgaaagcaac tttaggaata agtagatggg
     1141 agattcagta gaaataaaac taattctcca atttatagaa tgaaattgct tttatatcta
     1201 aattgctata taataatgat aaggaggaaa taagtatgtt aaaagaagta ttaaccgttg
     1261 caaaagttgc gaaaaaatct tcattatttt tgggtggtgt cgcatttggt acccttggtt
     1321 tgaaaatctt agcaagtaag gaagctaaaa aaggttattc taaagctttg gctaaggctt
     1381 acaagttgaa agacgagcta gatgcatctg tttctgttgt gaagcaacat ggagacgatg
     1441 tcttgcaaga tgccaaatat ttgtacgagc aagagaaaaa agaagagcaa ttagatagcc
     1501 ttataggtga ataatatgtc ttttaaagtg ctacatagag gataccaaca tatccgacta
     1561 tcatcttctt tttcacttac cttggatatt caagactatc ttcgttcctt ggcgagagat
     1621 gaaaagggga ttgagtctat ccagttttac atggatcaac agcactttac tctacgcata
     1681 aaagaaggct tttctgtatt agataatgca gaagcctttt taaaaagaat tgataaaggg
     1741 aaagtttctg agttgatgac tcttcccatt cgtagagaag agagtgctta ttctattgtt
     1801 tcaggtgcag cggttaagcg tgtacttttt cgtagttttg tgccgtatcc tattcgctat
     1861 atatggactt gttatcaggc tttgggttat attagagaag cctatcaaac actagcgcgt
     1921 aaggaactaa cgatggaagt cttggactgt tcggctattt tattgtcttt gtttatgaac
     1981 caatcaaaga cagctagcaa tatcatgttt atgcttgatt tggggaatca tttagatcag
     2041 tggtccttga aaaaaactgc aacagattta gaacagagtc ttcttgcaaa agagagcgat
     2101 gtattcctag tacagggcga tacggttgtt agtatcaaga gttccgatgt tcaaatagga
     2161 gatgtcttga tcttatctca aggaaatgaa attctgtttg atggacaagt agtttcaggt
     2221 ttaggtatgg tcaacgaaag ttccttgaca ggagagagtt ttccagttga aaaaagagag
     2281 tctgatttgg tttgtgcaaa tacagtatta gaaactggag agttacgcat tcgtgtaaca
     2341 gataatcaga tgaacagccg tattttacag ctgattgagt tgatgaagaa atctgaagaa
     2401 aacaagaaaa cgaaacaacg ctatttcatc aagatggcgg ataaagtcgt caaatataat
     2461 ttcttggggg ctgggctgac ttacctattg acaggttctt tttctaaggc tatttctttc
     2521 ctattggtcg atttctcctg cgctttgaaa atctctactc ctgtagctta tttgacagtt
     2581 atcaaggaag ggttgaaccg tgaaatggtg attaaggatg gagatgttct ggagaaatat
     2641 ctggaagttg atactttctt gtttgataag acaggaacaa tcacaactag ttatcctata
     2701 gttgaaaagg tgttaccttt tggagactat agtgaggaag atattctcag aatcagtgcc
     2761 tgtcttgagg aacacattta tcatcctatt gctaatgcca tcgtcaagca agctgagata
     2821 gagggaattg aacatgagga aatgcatggg aaactccaat atatcgcaag caaggggatc
     2881 aaatctcata tagatggcca accagttctt attgggaatt atgtcttgat gcaggatgag
     2941 cagattcata tcagttcaga acaaaatgct ttaattgaag agtacaagag tcactacaat
     3001 ctcttattct tggcttatca aaatgaattg attggaatgt tctgcattca tactcctttg
     3061 agaaaagaag caaaaacagc cttggataaa cttaaggcac aggggaaaaa attgattctg
     3121 gcaacaggag acaccttgat taggacagag gaattagtca aagatttgcc ctttgatcag
     3181 gtctatacag acttgaaacc tgatgggaaa tttgagttag tagagaaact gcagaaagca
     3241 ggtcacacta ttttgatggt tggagatgga ttgaatgact cagcggcttt aactctatca
     3301 gatatcggtg tggtgatgaa tgagagtgca gatatttcta agcagatgag tgatatctta
     3361 ttgttagata atcgtttgga tttcttccaa gagttggatt cgttatcatc atctttgcaa
     3421 acactcatca agaaaaatat tcaagatacc gttgtcgtaa atagtagttt gattggcttc
     3481 ggcttattta attggctcag tccttcaaat ctctctatct tacataatct aacaaccttg
     3541 cgcattgtcc tgcgtagcct gtctattaaa aatagataga tgagagttat taacagcaaa
     3601 aaggagttac ctttctcgag gttaactcct ttgtttatag ataaatgttg aatagtttag
     3661 taagtcaata caaagtattt tttcttccca cgacggataa cagttagttc attttctaac
     3721 ttatcagcgt cactcaagac atagtcaagg tcttggatgc ggtcgccgtt tacgtagatg
     3781 gctccgtttt ggacgtcttc acgggcttgg cgttttgagt taactatacc agatgagacg
     3841 agcagttcca cgatattgtt gtgttcgtct gcctgtactt ggtagtttgg cacaccacga
     3901 agtccttgtt tgagctcttt gacagaaagg tttttgatgt ttcctgcaaa gagttgctca
     3961 gtgatgttaa gtgcttcttt gtaggcttct tctccgtgaa caagtgtaac aacttcacga
     4021 gccaagactt tttgagccaa gcgttcgtgt ggcgctgctt caaattgttt acgaatatct
     4081 tcaatctcat caagtgacaa gaaagtaaag attttcaaga agcgaacagc gtcagcgtcc
     4141 atcacgttca tccagaattg gtacatttcg tatggagaag tcttttcggg attgagccag
     4201 acggcatttc cttctgattt accaaatttc ttaccagttg catctgtgat tagtggaaca
     4261 gtgataacgt gaccagtctt gtccgcctta cgacgaagca attcagtacc agctgtcata
     4321 tttccccact ggtcagaacc accgatttga agagtgacat tatggtcttg gttaaggacg
     4381 aagaagtcgt acccttgcat gatttggtaa gcgaactcag tgtaagaaat tcctgtttcg
     4441 atccgttttt taacagattc cttactcatc atgtagttga ccgtgaagta ttttccaata
     4501 tcacggagga agtcaatgaa gctgatgctg ccaaaccagt cgtagttgtt gaccatgaca
     4561 gccttgtttt cgccattttc aaagtcaaga aaacgagaaa gttgtccttg gatagacttg
     4621 acccagccat ctactgtgtc ttttgtttgg agactacgtt cagcatcttt gaaggacgga
     4681 tctccgatga gacctgtagc accgccaacg agcgcataag gtttgtgacc tgctagttgc
     4741 aagcgacgac ttgtcaagat tgcgacaagg tggcctaggt gaaggctgtc agcagttgga
     4801 tcgtagccag tataataaga aacttgacct tcttctaggg ctttacgcaa agcttcttca
     4861 tcagtcgttt gaaatatcaa accacgatct tttagctcat caaaaatgtg catgtgtctt
     4921 ttctcctttt taaaattatt gtttctacct attgtatcac aaacttgggc aatagcctag
     4981 cagaatagga aagaaagtgg ctattttatt gaaaatttcc cttttgtggt ataatagata
     5041 aagtgaggaa atccatgcaa aatcaattaa atgaattaaa acgaaaaatg ctggaatttt
     5101 tccagcaaaa acaaaaaaat aaaaaatcag ctagacctgg caagaaaggt tcaagtacca
     5161 aaaaatctaa aaccttagat aagtcagcca ttttcccagc tattttactg agtataaaag
     5221 ccttatttaa cttactcttt gtactcggtt ttctaggagg aatgttggga gctgggattg
     5281 ctttgggata cggagtggcc ttatttgaca aggttcgggt gcctcagaca gaagaattgg
     5341 tgaatcaggt caaggacatc tcttctattt cagagattac ctattcggac gggacggtga
     5401 ttgcttccat agagagtgat ttgttgcgca cttctatctc atctgagcaa atttcggaaa
     5461 atctgaagaa ggctatcatt gcgacagaag atgaacactt taaagaacat aagggtgtag
     5521 tacccaaggc ggtgattcgt gcgaccttgg ggaaatttgt aggtttgggt tcctctagtg
     5581 ggggttcaac cttgacccag caactaatta aacagcaggt ggttggggat gcgccgacct
     5641 tggctcgtaa ggcggcagag attgtggatg ctcttgcctt ggaacgcgcc atgaataaag
     5701 atgagatttt aacgacctat ctcaatgtgg ctccctttgg ccgaaataat aagggacaga
     5761 atattgcagg ggctcggcaa gcagctgagg gaattttcgg tgtagatgcc agtcagttga
     5821 ctgttcctca agcagcattt ttagcaggac ttccacagag tcccattact tactctcctt
     5881 atgaaaatac tggggagttg aagagtgatg aagacctaga aattggctta agacgggcta
     5941 aggcagttct ttacagtatg tatcgtacag gtgcattaag caaagacgag tattctcagt
     6001 acaaggatta tgaccttaaa caggactttt taccatcggg cacggttaca ggaatttcac
     6061 gagactattt atactttaca actttggcag aagctcaaga acgtatgtat gactatctag
     6121 ctcagagaga caatgtctcc gctaaggagt tgaaaaatga ggcaactcag aagttttatc
     6181 gagatttggc agccaaggaa attgaaaatg gtggttataa gattactact accatagatc
     6241 agaaaattca ttctgccatg caaagtgcgg ttgctgatta tggctatctt ttagacgatg
     6301 gaacaggtcg tgtagaagta gggaatgtct tgatggacaa ccaaacaggt gctattctag
     6361 gctttgtagg tggtcgtaat tatcaagaaa atcaaaataa tcatgccttt gataccaaac
     6421 gttcgccagc ttctactacc aagcccttgc tggcctacgg tattgctatt gaccagggct
     6481 tgatgggaag tgaaacgatt ctatctaact atccaacaaa ctttgctaat ggcaatccga
     6541 ttatgtatgc taatagcaag ggaacaggaa tgatgacctt gggagaagct ctgaactatt
     6601 catggaatat ccctgcttac tggacctatc gtatgctccg tgaaaagggt gttgatgtca
     6661 agggttatat ggaaaagatg ggttacgaga ttcctgagta cggtattgag agcttgccaa
     6721 tgggtggtgg tattgaagtc acagttgccc agcataccaa tggctatcag accttagcta
     6781 ataatggagt ttatcatcag aagcatgtga tttcaaagat tgaagcagca gatggtagag
     6841 tggtgtatga gtatcaggat aaaccggttc aagtctattc aaaagctact gcgacgatta
     6901 tgcagggatt gctacgagaa gttctatcct ctcgtgtgac aacaaccttc aagtctaacc
     6961 tgacttcttt aaatcctact ctggctaatg cagattggat tgggaagact ggtacaacca
     7021 accaagacga aaatatgtgg ctcatgcttt cgacacctag attaacccta ggtggctgga
     7081 ttgggcatga tgataatcat tcattgtcac gtagagcagg ttattctaat aactctaatt
     7141 acatggctca tctggtaaat gcgattcagc aagcttcccc aagcatttgg gggaacgagc
     7201 gctttgcttt agatcctagt gtagtgaaat cggaagtctt gaaatcaaca ggtcaaaaac
     7261 cagggaaggt ttctgttgaa gaaaaagaag tagaggtcac aggttcgact gttaccagct
     7321 attgggctaa taagtcagga gcgccagcga caagttatcg ctttgctatt ggcggaagtg
     7381 atgcggatta tcagaatgct tggtctagta ttgtggggag tctaccaact ccatccagct
     7441 ccagcagttc aagtagtagt tctagcgata gcagtaactc aagtactaca cgaccttctt
     7501 cttcaagggc gagacgataa ttctctaaat gaagtggcca atcaatggat tgccactttt
     7561 ttcttttcgt gttacaataa gggtatgaat cagtatcaga aaaagattgt taacggaaaa
     7621 atttattcgc tcctatccgg cttaatatgg ggaatctgtg gaattttagg agagtacttc
     7681 tttactcatt atcaggtgtc ttcgggctgg attacctcta tgcgtttgac actggcaggg
     7741 agtcttgtac tcatttggtc tgcaatacaa ttaaaatcgc aagtgctaga tatttggcga
     7801 gacaagaaaa attacctgcc ctttttagcc tatgctattt tggggatttt ttcagttcag
     7861 tattttttct atctctgtgt agaatactca aatgctacga cagcaactat tttacagttt
     7921 attagccctg tctttatcct cttttacaat cgcttggttt atcaaaaacg agcgtcaaaa
     7981 agcgctgttt tctatgtttt ggttgccatg ctgggtgttt gcttgatggc gacaaaggga
     8041 gatctctctc agttatccat gacgccgcta gctcttataa caggtttgct gagtgccatg
     8101 ggtgttatgt ttaatgttat cttgccccaa ccttttgcta agcgttatgg ttttgttcct
     8161 acggttgggt gggggatgat tttggcaggt ttgtttagca atgtcctctc gccggtttat
     8221 cagctttcct ttactcttga tatttggagt atcttgattt gcctcattat cgctttcttt
     8281 ggaacggctt ttgctttttt catttccatg aaggctgtgt ccttggtttc tcctttggtg
     8341 gttgccgtta tcagtgccag tgaacctctc tcttctgctc tcttgagtgt tttgttctta
     8401 ggattagtag tggattggtc cctccttcta gctatagcct tgattatttt acccatgata
     8461 tttttgtcta tagaagaagc gaaagaaagt agataaaaag tcttttctta attctaaaag
     8521 tgtgctatac tagaatagtc aataaaacac ggggagatag ttgtgaagag tgtttttagg
     8581 ttgtatcggt aggggcttgc ttttaccgac agcacgtttt atctcacaac cctaaaaaat
     8641 catacctaaa aacagacaaa aaggtttcaa atttgaggcc ttttttggta gaataggtat
     8701 cattataacg aaccaggagg cacctatgac tgctacaaaa atgaacgctc aagaaattat
     8761 ccaatttatc gccaatgctg aaaagaaaac cagtgtcaaa gtaacctttg aggggcaact
     8821 cgcaactgct gtgcctagct ctgttgtcaa actaggaaat gtcctattcg gagactggaa
     8881 ggacgtggct ccgcttcttg aaggtttggt agaaaatcaa gattatgttg tcgagcaaga
     8941 tgctcgtaat tctgcagttc ctttgctaga taagcgtgct atcaacgctc gtatcgagcc
     9001 aggtgctatt atccgtgacc aggtggaaat tggtgacaat gctgttatca tgatgggagc
     9061 tgttatcaat atcggtgctg aaatcggtgc tggaaccatg attgacatgg gtgccatcct
     9121 tggtggccgt gccatcgttg gaaaaaatag ccacgttggt gcaggtgcag ttttggcagg
     9181 tgtgattgag ccagctagtg ctgaaccagt ccgtgtcgga gacaatgttc ttatcggtgc
     9241 taatgcagtg gttatcgaag gagtccaaat cggtagtggt tcagttgtcg cagcaggagc
     9301 tattgttacc caagatgtcc cagaaaacgt ggtagtagca ggtgttccag ctcgtattat
     9361 caaagaaatt aatgcccaaa ctcaacaaaa aacagcgcta gaggatgcgc ttcgtacctt
     9421 gtaattgtaa aagtaaaaaa gaggcggaac cctttttcca gcctctttct gctatatcgg
     9481 aggacagata gatgttagat ttgattcaga ctagacgaga tttacaccag ataccagaga
     9541 ttggcttgga ggagttcaag actcaggctt atttgctgga tgtgattgag aaattgacta
     9601 cgggcaagga ttttgttcaa attcgtactt ggcggacagg gattttggtc tacctgcagg
     9661 gaagtcagcc ggagcgaacc attggttggc ggacagatat tgatggcctg cctatcgtcg
     9721 aacaaacagg tctacctttt gcttctcaac accaaggtcg catgcatgcc tgtggtcatg
     9781 attttcatat gaccattgcc ttaggctgtc ttgagcgcgc ccttgaggag caacccaaga
     9841 ataatctgct ctttctattt cagcctgctg aagaaaatga agctggtggt atgctcatgt
     9901 atgaggacgg tgcttttgga gattggttgc caaaccaatt ctatggtctc catgttcgtc
     9961 cagatttgaa agtcggccag attgcgacta atactcatac actctttgca ggaacttgcg
    10021 aggtgaagat ccgtttcaaa ggcaaaggtg gccacgcagc ttttcctcat gaagccaatg
    10081 acgccttggt ggcggctagt tactttgtga cccaagtgca gtcagttgtt agccgcaatg
    10141 tcaacccaat cgagggagca gtggtgacct tcggcgtttt tcaagctgga accaccaaca
    10201 atgtcatcac agacacagct tttttacatg gaactattcg tgccttgaca caggacatga
    10261 gtctcttggt gcaaaagagg gtaaaaacag ttgcagaagg ggttgcagct gcctttgata
    10321 tggaagtcga agtggaactc aaacaaggtg gatacctacc tgttgagaac aatccagcct
    10381 tggcgcgtga actgatggac ttctttgacg aaaaagacgg aatcgagttg attgatatcg
    10441 aatctgctat gacaggcgag gactttggtt atctcctttc aaaggttgat ggcgtgatgt
    10501 tttggctagg tatcgatagt ccctacgctc ttcatcaccc tcaaatgagt cctaaggaag
    10561 aagtcttagc tattggggtg gctgcggtgt ctagtttcct gaaaaagaag gcagcagaat
    10621 agagggcttg tatatgaaat cggaattacg caagcaagtc ttgcatgaaa tgaaggcttt
    10681 atctcaagaa caaaaacagg ctatagacca agctttaacc gagcgaattt tacaacaccc
    10741 cttttatcaa gaagccaagg tcatcgcaac ctacctctct ttttctcatg agtttcaaac
    10801 gcgggaactg attgagcagg cgctgaagga cggcaagaag gttttaatac ccaaaactta
    10861 tcccaagggg cgcatggact ttgtagtcta tgatccgcag cagttggtaa aaactgcctt
    10921 tgccttactg gagccacagg gagatttgga agtggtggat gtatctcaga ttgatttgat
    10981 tcatgttcct ggcctggctt ttacgacgga tggatatcgg attggatatg gtggaggtta
    11041 ttatgaccgc tatctggaac atttttctgg tcatactttg agtacggttc atccttgtca
    11101 aattcaggac tttatacctg aaaaccatga tattcctgtt caggaggtat taattgatga
    11161 aggaaatctt tgatagacgt taccctgtga cgagtttctt cctcttagtg acggccttgg
    11221 tatttttact aatgttggtc actgcaggcg gaaactttga cagggcagat acattatttc
    11281 gatttggagc catgtatggg ccagctattc gcctctttcc cgagcaggtt tggcgtctct
    11341 tgtctgccat ttttgttcat attgggtggg aacatttcat tgttaatatg ctttcacttt
    11401 attatcttgg aaggcaggta gaggagattt tcggctctaa gcagttcttt tttctctatc
    11461 tcttatcagg aatgatgggc aatctctttg tttttgtatt tagtcctaaa tccttagcag
    11521 caggcgcctc tacctctctt tatgggctat ttgccgcgat tatcattctt cgctatgcaa
    11581 ctcgcaatcc ttatatccaa cagctagggc aatcctatct gacacttttt gtggttaaca
    11641 ttattggaag tgttctgatt ccaggaatca gcctagcagg ccatatcggt ggtgcagttg
    11701 gtggcgcatt tctagcagtt atctttccag ttagaggaga aaaacggatg tataacacca
    11761 gccagagatt aggagcggta gtcttgttcg taggactcgc cattttgctt ttctacaagg
    11821 gaatgggaat gtgacgaagt tgaaagaaaa tttgtgtaat gaaaaaagat aaggcatttt
    11881 ttgaacctta tctttttatt ttattccttc tcagtcaatt cttttccaag ttggatgagg
    11941 taattcttca aatcctcttt gacttgtggg tgtttgaggg cgtagtcaat agatgttttc
    12001 ataaagccaa acttatcccc aacgtcgtaa cgagctcctg taaattcacg ggcaaaaaca
    12061 cgctgtgttt tattaagagt atcgattgca tcagtcagct gaatttcatt tcctgcacct
    12121 ggagcttggt tttcgaggat ttggaaaatt tcaggcgtga ggaggtagcg tccgataata
    12181 gcaaggtcgc taggagcgtc ctctggagct ggtttttcaa caaaggtttc aacactgtaa
    12241 agaccatctt ttccttcgcc ttgcggagca ataaccccgt aagcagatac ttcgtcatga
    12301 gggactggca tgacagcgat agtagacgcg tgggtacgct cgtagtcatc catgagttgt
    12361 ttggtaagtg gaacagcctt ttcgtctgtg atatccatca agtcatcacc aagcataacg
    12421 acaaaaggtt catttccgac gaaagccttg gcttgcaaaa cagcatctcc gagaccgcgt
    12481 ggatgagttt ggcggataaa atgcagacgc atgtcagttg ttttatcaac tagcttcaaa
    12541 agatctattt tccctttttc tttgaggtta tattccaatt cgaagtttga atcaaagtgg
    12601 tcctcaatag aacgttttga tttaccagtg acaactagaa tatcttcaat acctgatttg
    12661 agagcttctt ccacgataaa ctggatagtt ggtttgtcta cgattggcaa catttctttg
    12721 gcaagggcct tggttgctgg taaaaatcga gttcctagtc cagcagcagg gatgactgcc
    12781 tttctaactt ttgatgtcat aaaaatcctt tctatagagg gttaagacca ctcattttct
    12841 gctttaaatt cattgttcat gatgtcataa atggcatctt tgatattggt tccgtgataa
    12901 ataacttggt aaatagcctg tgtaatgggc atatagactc caagttcttg ggctagttca
    12961 taggctgctc gagtcgttga aattccttcg attaccatgc ccatattagc ttctatatca
    13021 gctagggatt ctcctcgtcc gagagcatct ccagctctcc agttacgaga gtggatggaa
    13081 gttcccgtta cgatcaaatc tcccacacca gataagccgc tataggtcaa tggactggcc
    13141 ccgagtgcta cccctaggcg ggtgatttct gctaaacctc gagcgatgat ggctgcctta
    13201 gcattatcac caaatccaag accatgtaaa gctccagcac cgacagcaat aatattttta
    13261 agagcaccag cagtttcaac cccgataaca tccgtattgg tataaagtcg gaagtagtga
    13321 ttactaaata gcttctgaac gtattgagct gtttgtaaat ctttagaagc agcagttatt
    13381 aaagttaggt cacgcacaat ggtctcttct gcatgactag gccctgaaac aacgacgata
    13441 tcactacgga gatgttcagg aatttcttct tcaagaatgg ttgataatcg tttatggcta
    13501 tcaggttcta atccctttga tgcgtgcatg atgataacct tatggtccaa ggtttgtgca
    13561 acttgctggg caacaagtcg tgtcactttt gttgggacaa caaacaaaat cgcatccaca
    13621 tctttcaatg tttctgctaa gtcggtgtag gcaatgatat tttcgtctag aacgacatct
    13681 ttaaagtagt gcttattagt atggtgtgta ttaatttcat tgatttgctc gggaagattt
    13741 ccccaaatac gtacctcgtg tccattgtca tttaagactt gtgaaagggc ggttccccaa
    13801 gaaccaggcc ccaagacggc gacggtttgt ttttccatga tttcctcctt gatagagttc
    13861 tttctcttat tctatcatat tctaggggat tttgcacttg cattactggg gtatggtgga
    13921 aaaatacaca aagaccgaga tgaagggata aactcttagt ctttagttaa agtgagaaga
    13981 aatctgatag atagtttgat tcagtatatg ctataaatag aggtaaacaa ttcaggaggt
    14041 tcttgtaggt tgcgagagtt tggcgaaaaa attaaaagat tacgtttggc taaaaaaatc
    14101 agtcgttcag aattttgtgg tgatgagtct gaattaagta tccgtcaatt aattagaatt
    14161 gaaaatggag aatccagacc aacactaaca aagttaaaat atatcgctga acgtttgggg
    14221 gttgaagatt acaagttgat gccaagttat atagagttgg ataaggaata cctagaattg
    14281 aagtatttct tgatgaggac tcctacatac gaagatgaaa ctatcgccca aaagaaagag
    14341 ggcgttttga taagattttt gaagagtatt atgataggct acctgaggaa gaaagattta
    14401 tcatcccaaa ttattcatat ctggcactaa cgaactacac agttcaaaaa ttaccagaaa
    14461 agctagttga aatactgtcc ttctggtgat tttgcttaat ttctgatgtt cgagacatga
    14521 ttgaggagat ttgtcttgat tttttcctaa aaaagggcag actcctccct tggtttgatg
    14581 ctcgattttt aaaataaggt gacttattct gttaggcatg ttttcccctg ccctctagct
    14641 ctatatggaa cagggagtat cagatttatc tggcagattg tatcaaataa ggcttgaaac
    14701 tgttttgaat aggcatatag acttgagaat ttgagaatat gtcgtctagc agtagagaca
    14761 tattttccgg caatatgaag gaagagacgt cgaaaacgct tgatagtcaa ggcctttact
    14821 tcatcaccag ctagctgctt taaaaagagg tagagattat aagccagaca gcccatcatc
    14881 atacgtactt cattcttaat catggtcgaa ctatctgtct tatccccaaa gaatcctgct
    14941 ttcctttctt tgataaagtt ttcagcattc cctcgttcac gatagaattg aaacagctcc
    15001 tcactatcta cactgcttaa attgttcaaa tgaacttttt tacaaccata aaaaagagca
    15061 cttaatcgta ctcttctctt ttaaggatta gttgaattaa ttcaaatcca ccagttctcc
    15121 tgtttctagg tagactaggc gttcacaaat gttagcaatg taatcagcga agcgttccag
    15181 atgccctatg atataaaggt attgagttcc attgggaatt gaggtttctt ggtctttcat
    15241 aagtccaatg atttcctttg ataaggcata ataatattgg tcaatctgtt catctttttg
    15301 agcaatacta atagcttttg aggcttggtg caaaggaaag gcaaccaata aatcggctag
    15361 catgctgagg gataatttac ccatttggtg taactgttct tcgtcagggg ctagttgatt
    15421 ttcttttagt tgcaaaacag ctttagcaat gcctgccata tggtctccca tacgttcaag
    15481 gtctgaacaa gaagacatga tgctaatcac aaatcgaagg tcagacactt gtggctgctg
    15541 caaggccaac aaacgggcac aggtcaattc gatagcgctt tgaccttggt tgatagcatg
    15601 atccttattg ataattagct ctgccatctc cttgtctttg gaggctaagg ccagtaaggt
    15661 ttttgaagct gtttcaagga caagttgccc tagtcctaaa aaggattgtt ctaattcatg
    15721 caattctaag tcaaattgat ttctcatagg tttttccttc cttatccgaa ccgtcctgaa
    15781 atatagtctt ctgtgcgctg atcttttgga ttggtaaaca cgtcaacggt gtctccaaat
    15841 tcgcaaattt ctcctgttaa gaaaaaagca gttttatctg aaatacgtga agcttgttgc
    15901 atgttatggg taacaatgat aatcgtataa tcctttttta gttgctgaat gaggtcttca
    15961 atttttaaag tggagatagg gtctaaggct gaagtcggct catccattaa cagaacatca
    16021 ggttctactg ctaaagctcg cgcaatgcaa aggcgttgct gctgaccgcc tgataaggac
    16081 atggcactct ttttaagatt atctttgact tcgtcccaaa tggctgcccc ttttaaagat
    16141 ttttccacta aggcatctaa ttgttttttg tctcgaatac catgtgtcct tgggccataa
    16201 gccacgttat catagataga catggcaaag ggattaggct gttgaaaaac catccctaca
    16261 cgcttgcgta gctgattaag gttgaatttg ctactataaa tatcttgctc atctaagagg
    16321 acttggcctt caatatggca agaaggaacc aaatcgttca tccgattaag ggtttttaga
    16381 aaagttgatt taccacaacc agatgggcct atcaaggcag taatctgtct ttctggtaat
    16441 tgaatcgaaa tattttttaa ggcttgaaaa tccccgtaaa ataagtctag gtgtctgact
    16501 gaaaatgttc ccatactagg aagctccttt cacaagtttt cgagataata agcttgatag
    16561 agtatttatc attaaaacag taataatcaa aatcacaccg gtagcatagg cttcattgac
    16621 atgtagcccc tcacttgaca gcatatacat atgcagggct agagaacgac ctgaagacat
    16681 gagactactt ggcgtattgg tagaggtacc taatgtatac atgagggcag ctgtctcacc
    16741 aacgatacgg ccaatagcta gtatcactcc agctaaaata cctggcatgg caactggtag
    16801 aacaattcta aaaacagtcc gtaacttacc tgccccaagt ccataacttg cttgacgcat
    16861 gctatcacta acagataaaa gggcttcttc tgttgagcga ataatgactg gcaacaccat
    16921 gatgactgag gttaagattc ctgataacag agagtattga aaacctaaga agactacaaa
    16981 gaagagcatg ccaaacagac caaaaacaat ggaaggaatc ccagacaagg tatctgaggc
    17041 caatcgcatg attttaacac aaagggaatc tttttttgta tattccacaa gataaaaacc
    17101 agcaaaaatc cctatgggca aggctaaaag aagagcacca aagaccagaa taacggtgga
    17161 aataatcgct ggcataaggg aaatgttctc agaagtataa gtccaagaaa agagggatag
    17221 acttagatga ggtaaacctt tgatgaggat aaaaccaatg attaaaaaga gagagccaaa
    17281 ggttaaagct gaaaaacaat aaacgagaag ttttagcagg tatttactca taagatgatt
    17341 ttcctttcaa gtaggcaaag taggcattaa tcaagagaat aaggaaaaag agaactgctg
    17401 aggttgcaat aagggcttcc ctatgctgac ctgatgcgta agccatttcc agaacaatat
    17461 tggttgttaa ggttctggtt cctgaaaaga gtccacttgg aataatcggc gggttgcctg
    17521 ccaccaaaat aactgccatg gtttcaccta ctgcgcgacc gatgcctaaa ataactgctg
    17581 ataaaatacc agatctcgca gctggcaaga tgacactaaa aatactccgt tcatgactag
    17641 ctcctagagc caagctacca gaataatacg ttttgggaac tgttcggata gcagattctg
    17701 acaaactgat aatggttggc aaaatcatta ttcctaatag taacgaagcg gttaggacac
    17761 tcatgccatt tcctaaaaag cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac
    17821 cataaacaat agatggaatg gccgccatca agttgatagc tgattttaag aagccataga
    17881 cgggctttgg acaataataa accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa
    17941 tcacaatcgc tcctaaggta attaataagg aaccaacgat cattggtaaa ataccatagc
    18001 ttgccggaat gttcgttggc gaccaatcac tgcctaataa aaaacgggca aagccgtagt
    18061 tagccatgaa aggtaagcca ttactaaaaa taaagaaaca gattagcaaa atagctacaa
    18121 cagctactgt tgcactcatg aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt
    18181 ttatcacatc ttgtcctttc tagtgaagaa agtaagggag atacgacacc tccctacttg
    18241 ccttctttat cttattgtac gatgaaacgt ctgcatctct ttagagattt atggagcaaa
    18301 cattttattt aatcttgtcc caggtggtta atttgccact aaaaacgtct gcaagttcag
    18361 ccatactgac ttggcttgcc ttattgtcat tattgaccac aacagcaata ccgtctaaag
    18421 caatagcatc atgggtgaga ctcttacctt cttcaggagt taattcccta gaaaccatac
    18481 caatatcagc ggttttctcc ttaacagcgg taatacctgc tgaagaccca ttagaggtaa
    18541 tatcaatcgt aacttctgga ttttcttttt tataagcttc tgctaatttt tccattaaag
    18601 aagatactga agtggaacct acaacagaca acttgcctga taagtgttgg cttgtatatt
    18661 ctgtggtttc agttttagct tcaataaatt tattatctgt gaccacttgt tgaccttgtt
    18721 tggagtggat aaagctgata aaatcttgac ctagcttgga aagattagaa gaccaaacaa
    18781 tgttgaaggg acgttgaaga gggtattcac catctaaaac tgtgtctcga ctagccttga
    18841 caccatcaat ctctaaagcc ttgacagatt tcgttaaaga tcccaaggag atgtagccga
    18901 tagcattagc attcccttga actgctgaga gaacaccttc tgtactattt tgaatcacag
    18961 ctgttttggc agtgttgtca atttttttat caccgtcttt tttgagaatc cctgtgattt
    19021 ctgtgaaggc accccgtgtt ccagagccat tttctcgtga aatcacctca atcgttcctg
    19081 aagctgactg tttggaagca gctgactgat tgccacaggc aacaagccca aatcctgata
    19141 agccaatggc tgcaagagta agcatttttt tgaatttcat aataatcacc tttatctcta
    19201 tgtatttttc ttgtgtaggc ttactacatt tatagtctaa caagtctttg taaaggttta
    19261 tccctgattc atgtaaagat tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct
    19321 gctcctaaaa atagctttaa aaattattag tcctgtgcga aagattggtt aggaagaaaa
    19381 atcgtgaagc aactgcctct gccaagctga ctcgtcaccg tgacttggcc acctaataat
    19441 tgactgagtt ctttgacaat ggcaaggcca agaccagtgc caccagtttg tctgcttcga
    19501 cctttattaa ctcggtaaaa acgttcaaaa atacgatcct gctctaattg actaatacca
    19561 atccctgtat ctgatacaga aatcttaatg ccttcgttca ccttttgggt cttgacctca
    19621 atttttcccc cttgttcagt gtaacggatg gcattggata aaagattgag taagatttgg
    19681 gaaagtaatt gactatctga tacgagggtg acatcatctg gcacctgcac ctttagctgt
    19741 aaatccttct tcttgagctg aggttgcaag ctttgagtca aatcctgtac aaattcagcc
    19801 aaagaaaggg tcgtccattg tataggcatt tgttgagcct tagataaggt aagaagatgc
    19861 tcaacaatat gctcaagacg caaactttct ttgtaaataa tgtctagaaa gtcatccttg
    19921 agcgcttctt cttcagctga catcccctta atggtttcag caaagccctt aattgaagta
    19981 actggtgtcc tcaattcatg ggaggcattt gagacaaagg ctaaatttaa cttttcataa
    20041 gttctaatcg ttgttaaatc atatagcaag acgagcacag cttccacaga ttgggtgggg
    20101 ctaaaaacgg gaactgctgt cacttctaaa atcaagtcac cctcatgaaa cccacttact
    20161 tcttgtttta accttgtttt ttgatcaaag gcttggtgaa ctaaattccg aatatccatc
    20221 cgtttgaggt catcaagtga acttatgtcg ccgtccacat cgggaaaata atgaggcaga
    20281 gagcgactgg ataataacat ctgaccttga gcggaaacta aaaacgtccc catggttagg
    20341 tgcgacagaa gaacctccat tgtttcggct aggtccttgt attgctgatc ctgttgggag
    20401 actttggttt ttaggccaga cacatactga gccaaagact ttaagtcttc ttgccctttt
    20461 tctaaaaagt attcactact ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc
    20521 catttccaaa ggcaaaagag ccagtagcca ccttgtccca aagaaagggc tagaagaaag
    20581 agaccgatgc ctttactgat ccaagttaat gccatccctg caatcagaat gaggctaaca
    20641 cttagattga ctagccaaaa ttgaaggtag cgtttcatct ataactcctt gaacttataa
    20701 ccataacccc gaatggttcg aataaattga ggggctttag gattgtcttc aattttttcc
    20761 ctcaacttac caatatgaac gtccaccaaa cgtgtttcct gcccaaagtc atacccccag
    20821 atacgttcca aaagacgctc tctagtcagt gtcatgttgg gatgtttcat aagatagagc
    20881 aagagttcaa attcttttgg ggtcaaactc agtaacttat tcgccttgta gacttcatga
    20941 cgctcagggt atactttcaa ggtcccaaat agccaagaat cgtcagcgat attatctgaa
    21001 tcatctcctt cttgttctcc tttagttcgc ctgaggacag ccttgacacg cgccagcaat
    21061 tctctagggc taaaaggctt ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc
    21121 ttatcaaatt catcactttt cgcagaaacc atcataattg gagttttgac gcctttggct
    21181 ctcagccgct tacaaacttc catgccatct aattgtggta acatgatatc aagcaagata
    21241 aaatcaaagg gttctgtttc tgccaaagct aaggccttcc gtccatttgt taccaattga
    21301 gtagaaaagc cttccttact taaatggtag tcaagcaatt tcagaatgtg ttcttcatca
    21361 tccactaata agacttgttt tgtcatctat tatctcctat tggtaacatt ataacacaat
    21421 tatcagaaat cctaacattg ctaaatcaga ttaaatttgc ttatcaagac tagtatctgg
    21481 tcaaacgctc aatcatctcc ttgtgctctg gataggtcgc cagtagatct accctttcaa
    21541 ataattcaaa atcctcaaat tcaaaaccag gagcaacaag acaagaaacc agagcataat
    21601 ccttatcaac tgttgatccc caaatagtgc ccttaggaac acagtagtga agttgttgcc
    21661 ctttggatat gtccaggcct aaagtgactg cttcgtagtg accatctgct gtaatcatgt
    21721 gaacagtaag tggggatcct gcatgaaaat accagatttc atctgctgtc aatcggtgaa
    21781 aatgtgaagg attcgtttct tctaataaga aataaatact ggtataaagc gcccttccct
    21841 taccagcaag gtttatagtg tctgaagctt tttttgtttg tctaaaatag ccaccttcaa
    21901 catggggagc taactctaga gttcttatca agtcttcttt atccatcggg gccaatgggt
    21961 tgaagtaact cttgttcaaa gtggttttac gatttcaaga actcctctca gttctgagga
    22021 cacggtaatg attgatgcga cggaagtaca aatcaatcgc cctaaaaaaa gaattagcga
    22081 atgattctgg taaaaagaaa tttcacgcta tgaaggctca agcgattgtc acaagtcaag
    22141 ggagaattgt ttctattgcc atgatatgaa gttgttcaaa atgagttgca gaaatatcgg
    22201 acaagctggt aaaatcttgg ctgacagtga ttatcaaggg ctcatgaaga tatatcctca
    22261 agcacaaact ccacgtaaat ccagcaaact caagccgcta acagctgaag ataaagccta
    22321 taaccatgcg ctatctaagg aaagaagcaa ggttgagaac atctttgcca aagtaaaaac
    22381 gtttaaaata ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa
    22441 tttgattgct ggtattatca atcatgaact aggattctag ttttgcagga agtctattat
    22501 ttggttaggt gaattagtga agtgtttagg caagtgtctc tggttacgac gtcatggcct
    22561 ctaaatcgat tatatttagg ggtcatgact agtgaagcag ttagctagtt cgcatataag
    22621 cggctagcgt ctaacaatta ggaactttag ttccaataac tttaagatta cgacgtttta
    22681 ggacataaat cgatcatatt tatgtcctaa aactagtgaa gcgcctagcc aaagtccgaa
    22741 taggatttgg cgttagttac ttagattgct ttgcaatcaa gtaactttgg cgatttacat
    22801 cttctctggc gcttctactc caagcaagcg aagggcttct ttgagaacga ctgcggttgc
    22861 gtagctgagg gctagacggc tgtcgcgttc tgggctttca tccaagatac gtgtatgtgc
    22921 atagtatttg ttaaaggatt gagccaggct aattgcaaat ttagcaatga tagaaggttc
    22981 aaagttatct gccgcacggt tgataatacg tgggaagtct tgaatgagtt taatgatttc
    23041 ccagctttca gtatcattca agctatagtt gccagctgtt tctggtttga aatcggcttt
    23101 gcgtaagata gattggatac gagcgtaggc atattgaacg taaggtccag tttcaccctc
    23161 gaaggatacc atagcttcta ggtcgaagtc gtatccattt gtacggtcag ttttgaggtc
    23221 atagaattta atggctccaa ccccaacagc atgtgctact tggtctttgt tttctagttc
    23281 aggattttta gcctcgattt ggaccttggc acggctaaca gcctctgcaa cagtaggctc
    23341 tagcaagatg acattccctt tacgagtaga gagcttcttc ccttcttttg taaccagacc
    23401 aaaaggaacg tgggtaatgt cgtcactcca atcgtagccc atctcttgca agacagcttt
    23461 gagttgttta aagtgggcag attgctcttg accaacgacg tagatagatt tagcaaattg
    23521 gtattcattt ttacggtaga gggctgcagc caagtcacgt gtgatataga gagttgcacc
    23581 atcagacttc ttgatgaggg ctggatgttc aattccatat ttctcaagat tcacaacttg
    23641 ggcaccttct gattcaagaa gtagtccttt ttcagaaaga atgtctacaa ctgcatccat
    23701 cttatcattg tagaaggctt ctccgttata gctgtcaaat tcaaccttca attcattgta
    23761 aaggcggtta aattccacta aactttcatc gcggaaccat tgccaaagag cgagagcttc
    23821 ctcatctcca ttttcaagtt tacggaacca ttcgcgcgct tcttcatcca agctagggtc
    23881 attttcagct tcagcgttga tgcggacata gagtttaagg agttcatcga ttggatgagc
    23941 ttttacagct tcttcgtcgc cccatttttt gtaggcaaca atcaacatcc caaattgttt
    24001 accccagtct cccaaatggt tgaccttgac cgtttgataa ccgatttttt ggaaaatatg
    24061 tgacaagcta tctccgataa cagttgaacg caggtggcca atagaaaatg gtttagcgat
    24121 attcggacta gacatgtcga taacaacatt ttcttgtttg ccaatatttt ggtcagcata
    24181 gtgttctttt tcagtggtaa cagcttgcaa tacttgagca gaaatggcag atttatcaag
    24241 gaaaaagtta acgtaaggtc ctgttgcgac aactttttca aaggcttggc tgttcatttt
    24301 ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt tcgactttgg caagagaaaa
    24361 agcagggaaa gcaatgtctc ccatttctga gtttttaggg gtttccagta actttaaaat
    24421 agcctcttgg tccaggctat caatgatgct agataattcg ctagcaatca attcttttgt
    24481 attcattaag agctcctttt tggacttttc t
//
LOCUS       DAVMHG010000015        24263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMHG010000015 DAVMHG010000000
VERSION     DAVMHG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.015"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            571..1185
                     /locus_tag="VYR08_000505"
     CDS             571..1185
                     /locus_tag="VYR08_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV3466080.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1478..2227)
                     /locus_tag="VYR08_000506"
     CDS             complement(1478..2227)
                     /locus_tag="VYR08_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV3466081.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            2360..4696
                     /locus_tag="VYR08_000507"
     CDS             2360..4696
                     /locus_tag="VYR08_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV3466082.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            4798..5475
                     /locus_tag="VYR08_000508"
     CDS             4798..5475
                     /locus_tag="VYR08_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV3466083.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            5462..6259
                     /locus_tag="VYR08_000509"
     CDS             5462..6259
                     /locus_tag="VYR08_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV3466084.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            6276..7379
                     /locus_tag="VYR08_000510"
     CDS             6276..7379
                     /locus_tag="VYR08_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV3466085.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            7635..8654
                     /gene="galE"
                     /locus_tag="VYR08_000511"
     CDS             7635..8654
                     /gene="galE"
                     /locus_tag="VYR08_000511"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3466086.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            8656..9621
                     /locus_tag="VYR08_000512"
     CDS             8656..9621
                     /locus_tag="VYR08_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3466087.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(9672..9878)
                     /locus_tag="VYR08_000513"
     CDS             complement(9672..9878)
                     /locus_tag="VYR08_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV3466088.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            9930..10406
                     /locus_tag="VYR08_000514"
     CDS             9930..10406
                     /locus_tag="VYR08_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV3466089.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            10415..11086
                     /locus_tag="VYR08_000515"
     CDS             10415..11086
                     /locus_tag="VYR08_000515"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV3466090.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            11217..11555
                     /locus_tag="VYR08_000516"
     CDS             11217..11555
                     /locus_tag="VYR08_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV3466091.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            11558..12187
                     /locus_tag="VYR08_000517"
     CDS             11558..12187
                     /locus_tag="VYR08_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV3466092.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            12427..13578
                     /locus_tag="VYR08_000518"
     CDS             12427..13578
                     /locus_tag="VYR08_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3466093.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            13891..14640
                     /gene="truA"
                     /locus_tag="VYR08_000519"
     CDS             13891..14640
                     /gene="truA"
                     /locus_tag="VYR08_000519"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV3466094.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            14630..15403
                     /locus_tag="VYR08_000520"
     CDS             14630..15403
                     /locus_tag="VYR08_000520"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3466095.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            15381..15842
                     /locus_tag="VYR08_000521"
     CDS             15381..15842
                     /locus_tag="VYR08_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3466096.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(16101..16304)
                     /locus_tag="VYR08_000522"
     CDS             complement(16101..16304)
                     /locus_tag="VYR08_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466097.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(16297..17379)
                     /locus_tag="VYR08_000523"
     CDS             complement(16297..17379)
                     /locus_tag="VYR08_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3466098.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(17505..18287)
                     /locus_tag="VYR08_000524"
     CDS             complement(17505..18287)
                     /locus_tag="VYR08_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV3466099.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(18287..19630)
                     /locus_tag="VYR08_000525"
     CDS             complement(18287..19630)
                     /locus_tag="VYR08_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV3466100.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(19729..21045)
                     /locus_tag="VYR08_000526"
     CDS             complement(19729..21045)
                     /locus_tag="VYR08_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV3466101.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            21238..22464
                     /locus_tag="VYR08_000527"
     CDS             21238..22464
                     /locus_tag="VYR08_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV3466102.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            22663..24237
                     /locus_tag="VYR08_000528"
     CDS             22663..24237
                     /locus_tag="VYR08_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3466103.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         6772 a         4678 c         5557 g         7256 t
ORIGIN      
        1 tcccttcggg gagatttttt ttgtttcact gaaagtttcg tacgatcttc gcctcgtcgc
       61 ctagtctata aacgatttat ctagcaagaa tcatgatgct aagggcgtca aaaatccgta
      121 tgaaaatagg gaaatgacac agtgttcgat gagcacaagg agtttcatct ttttcactag
      181 gattttagcc cgggctcaaa tcagctctct gacttcagag ggctttttta ttgaggtttt
      241 atcggtaaca attttggaga ctacaaaaac ctcaaacggt atcagctaat tacaccataa
      301 aggtgcgcag ttactcggct tttcaagccg agtagctgta tgcaagcccc ctagaagagc
      361 ccactcttta caaagtaaag tatagtatgt tatactttac atggaagtag tcactgaatt
      421 ccagttagaa attactttgt aactacgttt tgaggaggag taaaatgctt tcctacgttc
      481 gacattaccc actagcgata gctaaattaa tgtgtctgtg ctctcctaaa atctgctgat
      541 ttattactga ctaatacagg agtttttttt atgggacaga caatcatatc tgctattggt
      601 gtttatattt ccaccagtat cgattattta attattttaa ttattttatt tgcacagcta
      661 tcacagaata aacagaaatg gcatatttat gcggggcaat atctaggcac aggcttactt
      721 gtaggggcga gtttagttgc tgcttatgtc gttaatttcg tgcctgaaga atggatggtt
      781 ggattgcttg gtttaatccc tatctattta gggattcgct ttgcaattgt tggagaagat
      841 gcggaagaag aagaggaaga aattattgaa agattagaac aaagcaaggc aaatcaactg
      901 ttttggacag ttacattgct gacaattgcg tctggcggag ataatttagg tatctatata
      961 ccttattttg cttcgttaga ttggtcacag accctcgtgg ccttgcttgt gtttgtaatc
     1021 ggcataatta tcttttgcga gattagtcgg gtgttatcct ctattccgtt aatattcgag
     1081 acaattgaaa aatacgagcg aatcattgtg cccttagtat tcattctact tggactatac
     1141 atcatgtatg aaaatggcac gatagagact tttctgatcg tgtagatttt tttgtttcac
     1201 taggatttta gcccgagctc aaatcagctc tctgattttc agagggcttt ttcattgagt
     1261 ataaatggac aatcttaaag ttactctatt gtagagtggc ttttgatttg attaggagat
     1321 tcttataaat atagtaaaat gaaacaaaaa caggacaaat cgatcaggac agtcaaatcg
     1381 atttctaaaa atgttttaag aagtagaggt gtactattct agtttcaatt tgatatataa
     1441 atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct tctcttttct
     1501 tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg atagcgagga
     1561 taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt ggttttttat
     1621 cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga atacgattgg
     1681 cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg attggatttc
     1741 caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg ggaccggtgt
     1801 aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg ccccccttga
     1861 catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca ctttttttga
     1921 gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg atgggaaagg
     1981 cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct ttttttgcca
     2041 taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc caccaggtac
     2101 ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca gtattatgat
     2161 agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc aaataagtat
     2221 aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc aaaggagggc
     2281 tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt ctgttaaaaa
     2341 aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc aaagagaagt
     2401 caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca gtaccagcac
     2461 ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact ttgccattgc
     2521 tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg ttatctgctt
     2581 taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg acaagctcaa
     2641 tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg ctcttaatcc
     2701 tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga ttcctagtga
     2761 tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg gagaaagtga
     2821 tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag ccagtgggtc
     2881 agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac tcatgcttga
     2941 ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca agttggctgg
     3001 ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag ccttgctttt
     3061 aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt tgggaatgtt
     3121 gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga ttaagttggg
     3181 cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc gcgtggatat
     3241 gctctgtctg gacaagacgg gcaccatcac ccaaggaaag atgcaggtgg aggctgttct
     3301 tccactgacg gaaacttacg gtgaagaggc tattgccagc atcttgacta gctacatggc
     3361 ccatagtgag gataagaatc caactgccca agccattcgc cagcgttttg tgggagatgt
     3421 tgcttatcct atgatttcca atcttccctt ctccagcgac cgcaagtggg gggctatgga
     3481 gttagaaggc ttggggacag ttttcttagg ggcacctgag atgttgctgg attctgaagt
     3541 cccagaagcc agggaggcct tggagagagg atcacgtgtc ttggtcttag ctctcagtca
     3601 ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc tagccttgct
     3661 ggaaatcttg gaccccattc gagagggagc agcagagacg ctggactatc tccgttctca
     3721 ggaggtggga ctcaagatta tctctggtga caatccagtt acggtgtcca gcattgccca
     3781 gaaggctggt tttgcggact atcacagcta tgtagattgc tcaaaaatca ccgatgagga
     3841 attgatggcc atggcggagg agacagctat tttcggacgt gtttcccctc atcaaaagaa
     3901 actcatcatc caaacgttga aaaaagcggg tcatacaacg gctatgacag gggacggggt
     3961 taatgatatc ttggcccttc gtgaggcgga ttgttctatc gtgatggcgg agggagatcc
     4021 agcaacccgt cagattgcca atctggttct cttgaactca gactttaatg atgttcctga
     4081 gattctcttc gagggtcgtc gcgtggtcaa taacattgcc cacatcgccc cgattttctt
     4141 gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg ctttactagg
     4201 tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca tgattgacca
     4261 gtttgtggaa ggtttcccac cattcgtttt gacttttgag cgaaatatca aacctgtcga
     4321 gccaaacttc ctcagaagat ccatgcttcg tgccctacca agtgccctca tggtcgtgtt
     4381 tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt tagaaatctc
     4441 aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta gagcctgcat
     4501 gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct tcctagccac
     4561 agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag aacaaacgtt
     4621 gcctgtttat ggtgtcatga tgttggtctt taccgtgatt ttcatcctga ccagtcgcta
     4681 tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt aggtacaaga
     4741 ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg agagaagatg
     4801 atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat tttactagat
     4861 gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca aatcaaaagc
     4921 gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa tgttgaggct
     4981 cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc ttttgaagag
     5041 actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat tgctaggatt
     5101 ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca gcccaataat
     5161 cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt agcagaaaga
     5221 atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg acaaatgaag
     5281 ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag tccagtattt
     5341 gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ccctcggaca aatcccagaa
     5401 aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa ggaggtgctc
     5461 catgttagca agtgaagtga tccaagctta tgaagccttt tgccctcagg aattttctat
     5521 ggagggagac agctgtggtc tgcaaattgg cactttagac aagggtatcc aaagggtcat
     5581 ggttgccctc gatattcgtg aagagacggt ggctgaagcc attgaaaagg gtgtagactt
     5641 gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg ccagccgtcc
     5701 gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg tcagccatac
     5761 caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc taggaatcga
     5821 ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta ttgggaatat
     5881 tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg acctagatag
     5941 ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa gagtagcaat
     6001 ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg cagatgtcta
     6061 tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg gcttgttagc
     6121 attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag cactccttac
     6181 tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag catcgaccaa
     6241 tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc attattggag
     6301 caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac ctagaggtga
     6361 ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt atcagtcctt
     6421 ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg gctgactttt
     6481 atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt taccagcgtt
     6541 cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat caactggccc
     6601 tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac caagcttcag
     6661 ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct tctggtggag
     6721 cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat gtcaagctgg
     6781 tcaaagaaaa agtgactctg acactcttat catcaggcta ccagattggc gaagaggtgt
     6841 ttgatcaggt tattttggca acgggagctt ggttggggga catgttagag cctttaggtt
     6901 atgaagtgga tgtccgtcct caaaaaggac aactacgaga ttatcagctt gcccaagaca
     6961 tggaagatta ccctgttgtc atgccagaag gggagtggga tttgattccc tttgcaggtg
     7021 ggaaattatc cttgggcgct acccatgaaa atgacatggg atttgatttg acggtagatg
     7081 aacccttgct ccaacaaatg gaggaggcgg ccttgcctca ttacccagtt ttagctagag
     7141 cgacttcaag ggctgagcgt gtgggaatcc gtgcctatac cagtgatttc tctcccttct
     7201 ttgggcaggt gccagaattg gcaggtgtct atgtagccag tggactaggt tcatcaggcc
     7261 tcacaactgg tcctatcatt ggttaccatc tagcccaact gatccaagac aaggagttga
     7321 ccttggaccc tctaaattac ccaattgaaa actatgtcaa acgagtaaaa agcgaataat
     7381 actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca aagcactgct
     7441 ttgaggttgt gtatagaact gacgaagtca gtaaccatac ccatggcaag gcgacgttga
     7501 cgtggtttga attggatttt cgaagagtat aagcatttta ctgaaatttt atcaggtagt
     7561 ttaggatggc aaatgacatt ccctgtcaaa aatggtaaaa taagaaaaaa taatctgaga
     7621 atcgaggaaa aaagatgcaa gaaaagattt tggtaactgg tggtgccggt tttattggaa
     7681 cccacactgt tattgaattg atccaagcag gccatcaagt tgttgtggtg gataatcttg
     7741 tcaatagcaa tcgtaagagt ttagaagttg ttgaaagaat cacaggagtt gagattcctt
     7801 tctatgaggc agatattcgt gacacagtta ccctcagaga tattttcaag caggaagaac
     7861 cgactggtgt cattcacttt gctggtttga aggctgttgg cgaatcaaca cgtatccctc
     7921 ttgcctacta tgacaacaat atcgctggaa ctgtcagcct tttgaaagcc atggaagaaa
     7981 acaactgtaa aaacatcatc ttcagttctt ctgcgacagt ttacggggat ccgcacacag
     8041 tgcccatctt ggaagatttc ccactttcag tgaccaaccc atacggtcgt accaagctca
     8101 tgctagagga aattttgacc gatatttaca aagcagactc agaatggaat gttgtcttgc
     8161 ttcgttactt taacccaatc ggagcccatg agagtggtga tttgggagaa aatccaaacg
     8221 gtattccaaa caatctcttg ccatatgtga ctcaagtagc cgttggaaaa ttagagcaag
     8281 tgcaagtgtt tggagacgat tacgatacgg aagatggaac aggtgttcgt gactatatcc
     8341 acgttgtcga tttggctaag ggtcacgttg cagctttgaa aaaaatccaa aaaggttcag
     8401 gactaaacgt ttataacctt ggaactggta aaggttactc agttcttgaa attatccaaa
     8461 acatggaaaa agcggtggga cgtcctattc cttaccgcat cgtagaacgt cgcccaggtg
     8521 atatcgctgc ctgctactca gacccagcaa aagctaaagc agaactcggt tgggaagcag
     8581 aactcgacat cacccaaatg tgtgaagacg catggcgttg gcaaagcaag catccaaatg
     8641 gatttgaaga ctaagatgat gatttcaatc atcgtccctt gtttaaacga agaggaagta
     8701 cttcctcttt tttatcaggc tctggaagct ttacttccag atttggaaac agaaatcgag
     8761 tatgtctttg tcgatgatgg atcaagtgat gggaccttgg aactcttaaa ggcctatcgg
     8821 gagcaaaatc cggcagtcca ttatatttct ttctctcgaa attttggcaa agaagcagcc
     8881 ctttatgcag gcttgcaata tgcgacagga gatttggtgg tggtgatgga tgcagacctc
     8941 caagatcctc ctagtatgtt gtttgagatg aaaaatgtac tagacaaaaa tgtagacttg
     9001 gactgcgttg ggacacggag aactagtcgg gagggagaac ccttctttcg cagtttctgt
     9061 gctgttctct tttatcgcct catgcaaaaa atcagcccag tagctctgcc gtcgggtgtc
     9121 cgtgattttc gtatgatgag aaggtctgtg gtcgatgcca ttttaagctt gactgagtcc
     9181 aatcgttttt ctaagggact ctttgcctgg gtcggcttta aaacccacta tctggactat
     9241 ccaaatgtcg aaaggcaggc tggcaagacc agttggagtt ttaggcaact ctttttctac
     9301 tccattgaag ggattattaa tttttcagat tttcccttga ttatagcctt tgtagctggt
     9361 ctcctatctt gttttctttc tctgctgatg accttttttg ttgtggttcg gaccctcatt
     9421 ttgggcaatc cgacatcggg ttggacctct ctgatggctg tcattctctt tcttggaggc
     9481 attcaactct tgaccattgg gattctcggc aagtatatca gtaagattta tttagagact
     9541 aaaaaaagac cactttatct tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa
     9601 aataaagtga aaagactata attttacatg gaaatgtgct aaactagagg gagtggatta
     9661 tcgccattga tttaggcttc ctggtttcca atcagggcgc gagttgggca atttttaaca
     9721 gcctctaaga catcctgact aggagaaatt tctttttcca gttggtcagg gtcatcgtaa
     9781 aaacgcacga ttccattatc gtggtaatca aataaatcag aataagtttg gcaaagccca
     9841 caggcgatac atcgttcagg tataagtgtg attttcatgt tcatatttat attgtaataa
     9901 gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga accgtggcaa gaagatatct
     9961 atgatcaaga agaatcaaga gcagagcgtc ggcatcgaaa ccacggaggg gctgatagga
    10021 tggctaatcg tattttgacg atcctagcta gtattttctt tgtaattgtg gtggtgatgg
    10081 tcatcgttct catctatcta tcatcggggg gaagtaatcg cacagcagcc ttaaaagact
    10141 ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc aagcagttct cagcctgagc
    10201 agagttcaga gccagaatct acttctagta gttcagaaga agctgctaat cctgaaggaa
    10261 cgattaaagt tctcgcagga gaaggggaag cagctattgc cgctcgtgca ggaatctcca
    10321 ttgctcagtt agaggccttg aatcctgggc acatggctac aggatcttgg tttgctaatc
    10381 caggtgatgt tataaaaata aaataggagc cgatatgaaa acaattcaaa ttgctattga
    10441 tggtcctgct tccagcggta agagtacggt cgcaaagatt attgctaagg attttggatt
    10501 cacctacctt gatacaggag ctatgtatcg tgcagcgacc tatatggctc ttaagaacca
    10561 attaggagtt gaagaagtcg aagcccttct agccttgttg gaccagcatc caatcagctt
    10621 tggacgttca gaaactggag accagcttgt ttttgtagga gatgtggata ttacccatcc
    10681 tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg
    10741 tgagaaactg gtttctctcc aacaagaaat tgcccagcaa ggcgggattg tcatggatgg
    10801 tcgcgatatt ggaactgttg tattgccaca agcagaattg aaaattttcc tagtagcttc
    10861 tgttgatgag agagcagagc gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga
    10921 ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac aaggatagtc atcgtgagac
    10981 ttctcctctc aaacaagcag aggatgctgt ctaccttgat acaactggtt tgaacattca
    11041 agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg
    11101 atagattgat atcggttttt ttctaaattt gtcaaaatgc tgattttagg gtataatagt
    11161 tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga gaaatcatga
    11221 acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac ggtgctctac
    11281 tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa gtagaagaag
    11341 gtcttgtcgc tatcgatgcc aacggaaata aattgactga tggtgataca gtaactctca
    11401 tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa
    11461 atatccgcat cgtagaaggc gatcacaata tcgactgtaa gattgatggt tttggtgcca
    11521 tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact ataaacttgt
    11581 attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc ttgaaattac
    11641 taggcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc tcttggtaac
    11701 tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc
    11761 aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct gtgtgattta
    11821 ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt tcctttgcca
    11881 ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac agggtcaata
    11941 caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga tgtttgctct
    12001 ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc ctttccctta
    12061 cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc tgattatctt
    12121 tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca agagaaagtc
    12181 atcctaatac tcttcgaaaa tctcttcaaa ccacgtcagt ttccatctgc aacctcaaaa
    12241 cactgttttg aacaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    12301 aagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat gaggcttctt
    12361 cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa tatagaaaga
    12421 gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct cgttttgatt
    12481 acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca gggttattct
    12541 aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat gatgttactg
    12601 ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt gggcttgctg
    12661 attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt tgtctttgga
    12721 acacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat ttctattatc
    12781 agtgaacgct accaaggaaa aaggcgaatt cagatgttag ggctacgcgc ttctgcagag
    12841 gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt tggttggaca
    12901 gctatctttc tagcctatag tgctggattt ttggtgctgc ccctttatct gctctttgtc
    12961 ccttatggaa aatcaaagaa agaagtcaag aaaagagcga aggaagcaag tcgtttaact
    13021 cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt agtttgtacc
    13081 aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt gggggatgcc
    13141 cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc tggggtgagt
    13201 ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg tattaccttt
    13261 ggcttggggc aaattgtgat tgccttgtct tcatccttgt gggtggtagt agcaggaagt
    13321 gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa
    13381 cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg ctgtagtttc
    13441 ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca caatgggatg
    13501 ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt catgtaccta
    13561 cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca atgaaaatca
    13621 aagagcatag ttgattgagt ttggaatagt acgctgtagt tgctaaaata tttctagaaa
    13681 ttaatttgac ttttctaata gagttattca tatcttattt caatctacta tagaaagcta
    13741 gccgcaggtt gctcaaaata gtgttttgag gttgcagatg gaaactgacg tggtttgaag
    13801 agattttcga agagtatgag atttttatac ccatacaata tttgttttag gtccttgttt
    13861 acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac tatttcctat
    13921 gatggttatg cctttgctgg ctttcagcgc cagtctcatg cgcgtagcgt tcaggaagaa
    13981 attgaaaaaa ccttgaccag attaaataaa gggcaaacca ttactgttca cggtgctggt
    14041 aggacagata gtggggttca tgccctggga caggtcattc attttgacct gccttatcag
    14101 atggatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga tattgatgtg
    14161 atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaaaca tagcaagacc
    14221 tatgagttta ttgtggatag aggacgtccc aaaaatccta tgcgccgtca ctatgccacc
    14281 cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa gctagaggga
    14341 acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa ggttcgcacc
    14401 atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt taccttttca
    14461 ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct caaaatcggt
    14521 aacaaccgca tgccagtaga gcagattgat ctcatcttgg agaagaagga caggcaactt
    14581 gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta tgaagaataa
    14641 tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt cagctgattt
    14701 ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt
    14761 gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac atgaattaga
    14821 tagcttgcgt gatgtggaat ttgggggaat taagattggt cttctcccta ctgtcagtgt
    14881 ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg tgttggatcc
    14941 tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc aagagttgat
    15001 tcgctttttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag aattattatc
    15061 cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat tgcatgattt
    15121 aggagcgcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca aggctgtgga
    15181 tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc aaggccaaaa
    15241 tgctggtgca ggttgtacct ttgcctctag cattgccagt cacttgatta aaggtgataa
    15301 acttttgcca gcagtagaaa gctctaaggc tttcgtttat cgtgctattg cacaagcaga
    15361 tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc
    15421 tattaaccgc cctttctgtg gttctaggtt atttcttaaa aatcccaaca cctacaggaa
    15481 ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag
    15541 gagcggtagt cggaggacta gcaagtttct tgattgacct cttatcaggc taccctcagt
    15601 ggatgttctt tagcttggtc aaccatggct tgcagggatt tttcgcagga tttaaaggaa
    15661 aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtaggaggc tacgccttgg
    15721 gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctacca aatttcatgc
    15781 aaaatatggt agggatgatt gtaggattta ttcttagtca aagtatcaag aagattaagt
    15841 aatactcttc gaaaatctct tcaaaccacg tcagtttcca tctgcaacct caaaacactg
    15901 ttttgagcag cctgcggcta gctttctagt ctgattttca ttgagcataa agaggttgag
    15961 taaaaagtct ttcaaataag aaaaacgcat agtatcaggt gttgaatgaa cacctgatac
    16021 tatgcgtttt attgtgggaa gattttcctc attttctagt gaaattgaac tttttctaac
    16081 atcttttgat agatgacttc ttaatcaatt atcatttcca ttgttgtttc ttggggtttc
    16141 atcccttgtc tttgataaaa gcggagagca ccctcgttat cattccagac acttagggtt
    16201 agattattgc agccaatttc tttagcataa tagcagaaaa atgacaataa aatagcccct
    16261 agatattttg aaaggaaagc ataggggcta tatagtttaa tcaaaataaa gcaaatcttt
    16321 gctggtgctt gtaaagaggt cgaagccatc cttggtaaca acaccgcagt cttcaatacg
    16381 aacaccgact ttaccaggga tatagatacc tggttcaaca gagaagcaca tgccttcttc
    16441 gatgaccatg tcgtttcctt ccatgataga tgggaattca tggacatcca taccgatacc
    16501 atgcccgaga cggtggttga agtactcacc ataaccagct ttttcgatga cctcacgggc
    16561 agcgcggtcc acttcatgag cagtcacacc tggcttgata aagtcaagag cagcttgttg
    16621 ggcttcaaga gtcaagttgt aaatatcttt cttgaattgg tctggtttgc cgacagcgac
    16681 tgtacgagtc atatctgacg catagccatt gaccagaaca cccaggtcaa agaggagaag
    16741 agcatcattt tcaaccttat tagctgctgg aatgccgtgt ggattcgcag cattatcacc
    16801 agtcaagacc atggtatcaa agctcatttc gtaaccttca cgtttcatgg caaagtcaat
    16861 ttgtgcgatg atatctgtct cagtcttatc aagagaaata ttgtcaaaac caacatgaac
    16921 agccttgtca gcataaagac ctgcaaccat cattttttgc acttcatcag ctgatttgat
    16981 gaggcgcatg cgttggatac gaggagtgag gttgtcaaac tcagcagtct caaaaactgt
    17041 tttcaaacca tggtatttgg tcaagatgag attgtcaaac tcaacagcga cacgtttgaa
    17101 gtcaagttgt ggaagagcat gtttgatttt ttgccatgga ttttcagaat cgacatagcc
    17161 cactactggg aaggaaacgg tgctacttgc acgttctact tcaagagctg ggacaaagag
    17221 gagaggttcc tgatctgcta ggacaaagag gaacatttgg cgttcatggg gatcactgta
    17281 aaaaccagtg aggtaattga ttgtgacggg gtcagatacg acagcgacgt ctagtttttc
    17341 tgattcaaga tatgttagga tttgttgtaa tttagacata tgctaccttc tttctatccc
    17401 tctattttgg caaaaaatga gagaaaatgc aagggagaag ggtaaaagaa aagacaaaaa
    17461 actccgacaa gataacggag ttctttgata tcattttcct ttgtttaaga aaagtcagcc
    17521 ttgcttttga cgtcactgta ctcttcagcg atttcttggc tgaggatatc ctcataggca
    17581 gggagttgga tatcctgacc atatttcttc ttgagggcag tagtaactag gcgataagcc
    17641 agattggcat tacgagagag gataggcccg tggaagtagg aaccaaagac attcttataa
    17701 tgaacccctt caccgacctt ttcttcgttg tttccatttc catagacaac ctgtcccagc
    17761 ggtttttggt catcagagag gaaggtacgg ccttggtgat tttcaaatcc atagtaggtt
    17821 tcatcgaaat cttcattgtg aatcttgatg tcaccgataa aacggttatt ggtctggttg
    17881 agcgtgtagt gtcccatgac ccctagccct tcgatacgtt ttcctgaagc ttcaacataa
    17941 tattgaccca atagttggaa accaccgcag atagccagaa ctacaccgtc gttttggatg
    18001 tagttgtcaa tgctctcttt tttagcaggt aggtcgtctg caatgatact ttgttcaaag
    18061 tcttgaccac caccgaaaaa ggcgatgtcg tagtgatttt catcaaagtc atcatggaga
    18121 gaaacgatgt caacggtcac atgggctccc agtttttcag ccacatactt gagcatgagg
    18181 atgtttccat tgtccccgta ggtattcatg agatttccgt agaggtgggc aatgttgagc
    18241 tgataggggt aattgccatc ttttgaggaa agtgaagtat aaaccattag ttcatctcct
    18301 ttctaacaat ctgacgacta gccagcagtt cacgaaattc cagcatggca gtataagttg
    18361 ccagaatata ggcatgcttg cagtcttgat tctcaatggt cttgagaact tgctccagat
    18421 tactcgtttc agtgattttc tcagctggat agccagtcac tcggaggcga cgagcgattt
    18481 cagaatgacg aacaccgcca gcgttgattt caggaatgtc catgtcagtg atttgttcaa
    18541 agtctgcatc ccagatccag ctagtgtcaa ttccatctgc atagttggca ttaaggagga
    18601 cagataggct aaatggataa ggtgctagtt tgatcatttc gatagcttgg gttgcaccga
    18661 ctggattttt aatcaagaca agggtacatt ccttgtcacc gatatgaaag gtttcttggc
    18721 gtccaaagac agcacggctc ttgtcaaatc cctgtttgat gagttgcgaa tcggcaccta
    18781 ggaaacgggc gatggccaca gcagctaggg cgttatagat attatagagc ccgccgattt
    18841 ggataccgta ttcttggccg tctatgacaa agcgagagcg attgttggtc aactcaacca
    18901 gttttgtcaa acgatagtcg agatcaggac gtttgcatcc acaaccttca cagatatagg
    18961 cacccaagtt tgcataggta ttatgctcat atttgaggat gccttggcag tcaggacaga
    19021 gaatcccttc ggtattgtag tgagccagtt gggctggtcc tttttccaag tcaaaaccaa
    19081 aatactctat agggtttgga atagttggct tgtagaaaag tggactgtct ccgttaagga
    19141 gaacagtagc agttggaact ttccgaatgg cttccaatat catgttatag gtagtataga
    19201 tttcaccgaa acggtccatc tggtcacgga agatattagt aatgacaaaa agactaggct
    19261 ggatatagtc acagatacga gatagactgg cttcgtcaat ttcgaggacg gcaatatttt
    19321 tcccagtttt tgaagatttg gctgttagga aggttgttgc aatccctgta atcatgttgg
    19381 caccgcttgg gttggttaga acttgaccat aaacctcttt taaaatgccg acagtgaggg
    19441 cagttgtcag ggtttttcca tttgttccag tgacaacgac aatctcgtag ttcttagcta
    19501 ggttttgtaa aatatcttta tcaaattgaa gggcgacttt acctgggagc gtacttccac
    19561 gtccaagacg gcttaaaacg aagtgggaag aacgcccagc aagaaggccc aaagtagttt
    19621 ttaagttcat gtttctatta tatcacaaaa gagggaaaag acagatttgg tgtttcgtag
    19681 aagtaaaaac agcccaaaga gggctgtcag ttaagatgag atttcaactt aaatcgcaaa
    19741 caagtcattc aagttctcag ccaaggttgg gtgagtgaag atttgttttg tgaagtaagt
    19801 gtaaggaatc ttgttgtcca tagcaacagt aatgatgttg atgatttctt gagaaccttc
    19861 tgagaagatg cttgctccaa gaatttcttt tgtttcagta ttgacaacag ctttgaaggc
    19921 accgcgaagg tctccattta cgtgaccacg aggcattgct gcaacgggga tttccttaac
    19981 agcgtatgga agtttcaaat cagctgcttg gctttcagtc aaaccaactt gtgaaagtgc
    20041 aggtgtgatg aacatagtat ttggcacatt gagacggtct tcaagtgtgt agctgccatc
    20101 tccagcaagg tagctgtaaa caacacggaa gtcatcaagt gaaatgtaag taaattgaag
    20161 gccaccgttg acatctccaa ctgcaaagac accaggaacg tttgtttgac agtgtttgtc
    20221 tactttaata gcaccacgtt cagttagttc aatatctgta ttttcaagtt gaagtggttc
    20281 tacatttggt ttgcgtccag ttgcgtagag aagggcgtcg aaacggtaag tttcgtcttc
    20341 agttacgaca agcacttggt caccatcgtt tttgatttca gtagtatgga tattttgaag
    20401 caattcaatg ccatcttctt ccatgtattg tttagcaaga gctgcgatgg aaggttctgc
    20461 acgaggtagg aatgtatcca aggcatctag gactgtgacc ttgcttccaa gtttgttgta
    20521 aaggccggca aattcaagac cgatatttcc gccaccaagg attccaagtt tttcaggtaa
    20581 tttgtccaag ctttggatac ctgttgagtc aaagatgttt ttgcttgtag caagtccagg
    20641 gattggcaag acgtttgaaa cagcaccagt gttgatgacg attgtttcag cagtcagttc
    20701 tttcttttca tcaccagctt ggatttcgat gactttattt gaaaggaagt gagcttccgc
    20761 atcaaagata tctacgcctg taccagcaac agtcgcatag tttttaccgt tgaggcgacc
    20821 agtgatcgtg tttttagtag caatgacttc ttcaaaagac aagtcctttt cagcagcaac
    20881 tagcaaggtt ttagttggga tacaaccaat gttgatacaa gttccaccgt acatagcctt
    20941 gctgcgttca acgagggcaa cttttttgcc agctgaagcc aatttacctg ctagtgtttt
    21001 accagcttta ccaaatccaa taacaattaa atcgtatgtt aacatttatc ttcctcctat
    21061 tcgaatttca ttctagcaca agaaaaaaac ttttgcacaa ttctgctacg gaaaagacag
    21121 aaaaagataa aaacgctttc cagacaaaaa actagtaatt ttttgacaag cttgataaac
    21181 taaaatccta tctattttta tataagatag aataaatgtt cgaaaatagg aggttttatg
    21241 aaatcgaatc gttatattat tgcctttgct ggggtcattt tacacttaat gctgggttcg
    21301 acttatgctt ggagtgttta tcgtaaccct attattgaaa aaacgggatg ggatcaggct
    21361 tctgttgcct tcgcctttag tctagcaatc ttttgtttgg gcttatcggc tgcatttatg
    21421 gggcgtttgg tagaaaaatt tggtccgaaa gtcatgggaa gtctatctgc ttttctatac
    21481 gcaggtggaa atatcttaac aggatttgca atagaccgtc aggagctgtg gttgttgtat
    21541 ctcgcttatg gcattttagg tgggcttggt ttgggagcag gctatattac ccctgtgtcg
    21601 acgattataa aatggtttcc tgataaacgt ggtctcgcaa caggtttagc gattatgggg
    21661 tttggttttg cttctttatt gactagtccc atagcgcaac acctcatcgc aggggtaggg
    21721 cttgtagaaa ctttttatat tttaggagca agttacttta ttatcatgct cctagcttca
    21781 caattcatta agcgtccaaa tgagcaagag cttgcaattt tatcttcttc agggaaagaa
    21841 aaaacagcct ctttgacgca aggaatggct gcaaatcagg ctctaaaaag caatcggttt
    21901 tatatgcttt ggattatttt ctttatcaac atagcttgtg gtttaggctt aatttcagcg
    21961 gcatcgccaa tggcacagga gatggctggc ttgtctacaa gtcatgcagc agtaatggtg
    22021 ggtgttttgg ggattttcaa tggatttggt cgcttgctct gggcgagttt gtctgactat
    22081 atcggtcgcc ctctaacctt tagtatatta ctgcttgtca atcttttctt ttctctctca
    22141 ctttggctct ttacagattc cgttttattt gtagttgcta tgtctatttt gatgacttgc
    22201 tatggagctg gtttttcttt gattccagct tatctcagtg atatttttgg aaccaaggaa
    22261 ttggccgctc tgcatggtta tattttaaca gcttgggcaa tggctggttt agcgggacct
    22321 attttattag cagagactta taaaatggct cattcgtaca cacaaacctt gttcgttttt
    22381 ctcattttat acagtatcgc cttggctttg tcttattatc taggtcgttc aatcaaaaaa
    22441 gaaagtcaaa aaccgcttac atgattttct gtagaaatgg ggctatcttt tgctctggtc
    22501 ttgtgttata ctagataggt tgcaaagaaa acagtacttt tcttttgtgg aaaagaagca
    22561 acacgatttt atatctagtt atatgaaaat acgtcataaa aagaaaagta taaactaagc
    22621 cctatagggt ggcttcgcac cacctttaga aagaagaata acgtgaaatt taatgaatta
    22681 aacttgtctg ctgatttgct agcagaaatt gaaaaagctg gttttgtaga agctagtcct
    22741 atccaagaac aaactattcc tttggccctt gaaggcaagg atgttatcgg tcaagctcag
    22801 actggtacag gaaaaactgc agcctttggc ttgcctaccc ttgaaaaaat ccgtacagaa
    22861 gaagcgacta tccaagcctt ggtcatcgct ccaactcgtg aactagctgt ccaaagtcaa
    22921 gaagaactct tccgctttgg tcgtagtaag ggagtcaaag tccgttcagt atatggcgga
    22981 tcaagcattg aaaaacaaat taaggctctt aaatctggtg cccatatcgt ggtgggaact
    23041 ccaggtcgcc tcttggactt gattaaacgc aaggccttga aattacaaga cattgaaacc
    23101 cttatccttg acgaagcgga tgaaatgctt aacatgggct tccttgaaga catcgaagcc
    23161 attatttccc gtgtacctga gaaccgtcaa actttgcttt tctcagcaac tatgccagat
    23221 gccatcaaac gtatcggtgt tcagtttatg aaagcccctg aacatgtcaa gattgcggct
    23281 aaggaattga caacagaatt ggttgaccag tactatatcc gtgttaagga acaagaaaaa
    23341 tttgacacca tgactcgtct catggatgtg gcacaaccag aactcgctat tgtatttggt
    23401 cgtaccaaac gccgtgtgga tgaattgact cgtggtttga aaattcgtgg cttccgtgca
    23461 gaaggaattc atggcgacct agaccaaaac aaacgtcttc gtgtccttcg tgactttaaa
    23521 aatggcaatc ttgatgtttt ggttgcgaca gacgttgcag cgcgtggttt ggatatttca
    23581 ggtgtgaccc atgtctacaa ctacgatatt ccacaagatc ctgagagtta tgttcaccgt
    23641 atcggtcgta caggtcgtgc tggtaagtca ggtcaatcta ttacttttgt tgctccaaac
    23701 gaaatgggtt accttcaaat cattgaaaac ttgactaaga aacgcatgaa aggtctcaaa
    23761 cctgcaagtg tagaagaatc cttccaatca aaaaaacagg tagctctcaa gaaaatcgaa
    23821 cgtgattttg cagatgaaac cattcgtgcc aactttgaga aatttggtaa ggatgctcgc
    23881 aaattggctg ctgagtttac tccagaagaa ttggcaatgt atatcttgag tctgacagtc
    23941 caagacccag atagccttcc agaagtggag attgcacgtg aaaaaccact accgtttaaa
    24001 ccatcaggta atggtttcgg tggtaaagct aagggaggtc gtggaggccg tcgtggggac
    24061 gaccgtcgag agcgtgatcg ccgtggcaat ggtcgccgtg atgagttcaa aaaaggaagt
    24121 cgtggcaacg atcgttttga taaggaaaaa cgttaccgta aggataataa aaaaccacgc
    24181 aatactttaa gcgaaaagca aacaggcttt gttattcgta acaagggcga taaataagaa
    24241 aaagcggttc aaaatgaatc gct
//
LOCUS       DAVMHG010000016        24157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMHG010000016 DAVMHG010000000
VERSION     DAVMHG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.016"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            584..2737
                     /locus_tag="VYR08_000529"
     CDS             584..2737
                     /locus_tag="VYR08_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV3466104.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2750..4099
                     /locus_tag="VYR08_000530"
     CDS             2750..4099
                     /locus_tag="VYR08_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV3466105.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSFYQSYK
                     SQGEENPQTKVQVVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4269..4976
                     /locus_tag="VYR08_000531"
     CDS             4269..4976
                     /locus_tag="VYR08_000531"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV3466106.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5033..8758
                     /locus_tag="VYR08_000532"
     CDS             5033..8758
                     /locus_tag="VYR08_000532"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV3466107.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8851..10293
                     /locus_tag="VYR08_000533"
     CDS             8851..10293
                     /locus_tag="VYR08_000533"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV3466108.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNTASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10330..11352
                     /locus_tag="VYR08_000534"
     CDS             10330..11352
                     /locus_tag="VYR08_000534"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV3466109.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11349..11894
                     /gene="purN"
                     /locus_tag="VYR08_000535"
     CDS             11349..11894
                     /gene="purN"
                     /locus_tag="VYR08_000535"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV3466110.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11978..12487
                     /locus_tag="VYR08_000536"
     CDS             11978..12487
                     /locus_tag="VYR08_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV3466111.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEY
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12512..14059
                     /gene="purH"
                     /locus_tag="VYR08_000537"
     CDS             12512..14059
                     /gene="purH"
                     /locus_tag="VYR08_000537"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV3466112.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLGSDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14181..15443
                     /gene="purD"
                     /locus_tag="VYR08_000538"
     CDS             14181..15443
                     /gene="purD"
                     /locus_tag="VYR08_000538"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV3466113.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAADTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15845..16333
                     /gene="purE"
                     /locus_tag="VYR08_000539"
     CDS             15845..16333
                     /gene="purE"
                     /locus_tag="VYR08_000539"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV3466114.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEAYSRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16320..17411
                     /gene="purK"
                     /locus_tag="VYR08_000540"
     CDS             16320..17411
                     /gene="purK"
                     /locus_tag="VYR08_000540"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV3466115.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALAESADCVLEEFVNFALEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17421..17648
                     /locus_tag="VYR08_000541"
     CDS             17421..17648
                     /locus_tag="VYR08_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV3466116.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17711..19009
                     /gene="purB"
                     /locus_tag="VYR08_000542"
     CDS             17711..19009
                     /gene="purB"
                     /locus_tag="VYR08_000542"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV3466117.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19064..23002)
                     /gene="strH"
                     /locus_tag="VYR08_000543"
     CDS             complement(19064..23002)
                     /gene="strH"
                     /locus_tag="VYR08_000543"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV3466118.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGSPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23317..24033)
                     /locus_tag="VYR08_000544"
     CDS             complement(23317..24033)
                     /locus_tag="VYR08_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3466119.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6583 a         4775 c         5722 g         7077 t
ORIGIN      
        1 tctaaaaata taaccttaag ttttagcatt ttactttgtt atttatttaa tccatcggat
       61 ataaagtttg cctatttcag cgtattgaca gtattactaa ttgggataac aaattatgcg
      121 agatttaaaa atttaagatt gacagtagtg ttttctgtac catttttatt taatgctata
      181 atgtctatta taaatagttt ttagaaatac taaaattttt ttctttactt aataatgata
      241 tgaaatcatt tcggttttgt taacttgtaa ttcttatatt ttctaggtag aacgtttgtt
      301 ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat ttgggaagga
      361 agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag tttatactct
      421 tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg actgacttcg
      481 tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
      541 tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat ttgggaaacg
      601 tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag ccatggtttt
      661 tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta agacgaccat
      721 ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt ttgagacgcg
      781 agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc cttttgttgc
      841 ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgacggggc aggataagga
      901 tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc cacgtgagcg
      961 ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag actataagcc
     1021 tcataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga agcagcgtgg
     1081 cttgattgcc aatatcgttt tggcaacact cttggtaacc gtgattaaca ttgtgggttc
     1141 ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt cgacactagg
     1201 gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt cttacgctca
     1261 ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt tgtcctatat
     1321 caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca cgcaggacag gggagatcgt
     1381 gtctcgtttt acagatgcta acagtatcat cgatgcgctg gcttcgacca tcctttcgat
     1441 tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt ctattttcac aaaataccaa
     1501 tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct ttgcctttat
     1561 gaagccgttt gaaaagatga atcgggatac catggaagcc aatgcggttc tgtcttcttc
     1621 tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg
     1681 ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta cctatagtcg
     1741 agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttgctta atgtcggcat
     1801 tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc agttgattac
     1861 ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca atctgcaaac
     1921 caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc
     1981 tgagtttgag gagaagaaaa cagttgagga tttgagcttg atgaagggag atatgacctt
     2041 caagcaggtt cattacaagt atggctatgg tcgagacgtc ttgtcggata tcaatttaac
     2101 cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta agacgacttt
     2161 ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc tgggtggtgt
     2221 caatctcaat cagattgata aaaaagccct gcgccagtac atcaactatc tgcctcaaca
     2281 gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca aggaggggac
     2341 gacacaggaa gatatcttac gggcggtcga attggcagag attcgagagg atatcgagcg
     2401 catgccactg aattatcaga cagaattgac ttcggatggg gcagggattt caggtggtca
     2461 acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct tgattttgga
     2521 tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata atctcattgc
     2581 tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc ggacagagaa
     2641 ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg atttgcttgc
     2701 acagggaggc ttttacgccc atttggtcaa tagctagaaa gaggagagga tgaaaccaga
     2761 atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca gtagtgtgat
     2821 tgtacccatg gcccttctgc tcgtgttttt acttggcttt gcaactgttg cagagaagga
     2881 gatgagtttg tccactagag ctactgtcga acccagtcgt atccttgcaa atatccagtc
     2941 aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg ttaagaaggg
     3001 ggatcttttg gttcaatacc aagaaggggc agagggtgtc caagcggagt cctatgccag
     3061 tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa agagcctgca
     3121 agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca cctttcgcga
     3181 ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg agaccatcgc
     3241 gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca tcagtcaaac
     3301 agaggctaaa attcgcgatt accagacagc taagtcagct attgaaacag gtgcttcctt
     3361 ggccggtcag aatctagcct actcttttta tcagtcctac aagtctcagg gcgaggaaaa
     3421 tccccaaact aaggttcagg tagttgcaca ggttgaagca cagatttctc agttagaatc
     3481 tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag cctatgcgtc
     3541 agggttaagc agtcaattgg aatcccttaa atcccaacac ttggcaaagg ttggtcagga
     3601 attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg tacagggaaa
     3661 tcttttagac aaggggaaaa ttacggcgag tgaggatggg gtgcttcatc ttaatcctga
     3721 gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt atccatcttt
     3781 ggaaagagaa ggtaaagcca aactcacagc ttatctaagt tcaaaagatg tagcaagaat
     3841 caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc aacttttcct
     3901 agattctact attacaagta ttgatgcgac agctactaag actgagaaag ggaatttctt
     3961 taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta ggtacggggt
     4021 ggaaggccgc ttgcagatga ttacgggcaa gaaaagttat ctacgttatt atttggatca
     4081 atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta aactgtgaga
     4141 aagattcttc ttgcagtttt ttctttacaa tttttggaaa acatctgcta tttattcggt
     4201 taaattcttg tattttttgg ttttttgtgg taaaatgtgc tcaagtaata cgaaaggcga
     4261 actttaaaat gtcaaaacaa ttgatctatt cgggaaaagc taaagatatc tatacaactg
     4321 aggatgaaaa tcttattatt tcaacttaca aggaccaggc gactgctttc aacggtgtca
     4381 agaaggagca gattgcaggt aagggagtct tgaataatca gatttcatct tttatttttg
     4441 agaaattaaa tgcggctggt gtggcgactc actttgtgga gaaactttca gacacggaac
     4501 aactcaataa aaaggttaag attattcctt tggaagtcgt gctccgcaat tatactgctg
     4561 gttccttttc aaaacgtttt ggtgtggatg agggaatcgc cttggagact ccgattgtcg
     4621 aattttacta caaaaatgat gatttggatg atccatttat caatgatgag catgtgaaat
     4681 tcctacagat tgcggatgac cagcagattg cctacttgaa ggaagaaacg cgtcgtatca
     4741 atgaactatt gaaagtctgg tttgctgaga ttgggcttaa attgattgac tttaagctag
     4801 agttcggttt tgacaaggat ggcaagatta tcttggcaga cgaattttca ccagataact
     4861 gccgcttgtg ggatgctgat ggcaaccaca tggataagga tgttttccgt agaggattgg
     4921 gagaactaac cgacgtttat gagattgttt gggaaaagtt gcagggttta aaataacaac
     4981 ctcaaggctg tttgggaata ttgcaagagc tgaaataaag gaataagaat tgatggataa
     5041 acgtattttt gttgaaaaaa aggctgattt tcaggtcaag tcagagagtt tggttagaga
     5101 gctccagcac aacttgggac tgtcaagctt gaaaagtatt cgtattgtgc aagtatatga
     5161 tgtatttgac ttggttgagg acttgtttgc acctgcagag aagcacattt tctctgagca
     5221 ggtaaccgac catgttttag atgaagtatc tgtgcaggcg gatcttgcta actatgcttt
     5281 ctttgccatt gaaagtctac cagggcagtt tgaccagcgt gcagcttcgt cacaggaagc
     5341 cttgcttttg ttgggaagtt cgagtgacgt gacagtcaac acagcccaac tttacttggt
     5401 gaataaagat attgatgcga ctgagttgga agctgtcaaa aactacctgc tcaatccagt
     5461 tgattctcgt ttcaaggata tcacgatagg gattgccaag caggagtttt cagagtcaga
     5521 caagaccatt ccaaaattgc ctttctttga aagctataca gcagaagact ttgctcgcta
     5581 caaggccgaa caagggatgg ccatggaagt ggatgatttg ctctttatcc aagactactt
     5641 taagtcaatc gggcgcgtgc caactgagac tgaactcaag gttttggaca cttactggtc
     5701 tgaccactgc cgtcatacga cttttgagac agagttgaaa cacatcgact tttcagcttc
     5761 taaatttcaa aagcaattgc agtcaaccta tgacaagtat attgccatgc gcgaggaatt
     5821 aggtcggtct gaaaaaccac aaaccttgat ggatatggcg actattttcg gtcgttatga
     5881 gcgtgctaat ggacgattgg atgatatgga agtctctgac gaaatcaatg cctgctcagt
     5941 tgaaattgaa gtggacgttg atggtgtcaa ggaaccttgg ctcctcatgt ttaaaaacga
     6001 aacccacaac catccaacag aaattgagcc atttggtgga gcggctacct gtatcggtgg
     6061 agctattcgt gatccgttgt caggccgttc ctatgtttac caagccatgc gtatttcagg
     6121 tgctggtgat attacagcac cgatttcgga aactcgtgct gggaaattgc cacaacaagt
     6181 catttctaaa acagcaactc atggttattc ttcatatggt aaccagattg ggcttgccac
     6241 aacctacgtt cgtgaatact tccacccagg ctttgtagct aaacgtatgg aacttggtgc
     6301 cgttgttggt gcggctccca agggcaatgt tgtccgtgaa aaacctgaag caggtgatgt
     6361 gatcatcctt ctcggaggta aaacaggtcg tgatggtgtc ggtggtgcga cgggctcttc
     6421 taaggttcaa acagttgagt ctgtagagac tgctggtgct gaggttcaaa aaggaaatgc
     6481 catcgaagaa cgcaagattc agcgcctctt ccgtaatggc aatgtcactc gtctgatcaa
     6541 gaagtccaat gactttgggg caggcggtgt ctgtgtggct atcggtgaat tggcagacgg
     6601 tcttgaaatc gacctcaaca aggtgcctct taaataccag ggcttgaatg gtacagaaat
     6661 tgccatctct gaatcacaag aacggatggc ggtcgtggtt cgtcctgaag atgtggatgc
     6721 cttcgttgcc gaatgtaaca aagaaaatat tgatgctgtt gtggtggcga cagtaactga
     6781 aaaaccaaat cttgtcatgc actggaatgg tgagacaatc gttgacttgg agcgtcgttt
     6841 ccttgacacc aatggtgtgc gcgtggttgt cgatgccaaa gttgtggaca aggatgtcaa
     6901 actcccagaa gagcgtcaaa catctgctga aacactggaa tcagataccc ttacggttct
     6961 atctgacctc aaccatgcaa gtcaaaaagg attacagact gtctttgact gctctgttgg
     7021 acgctcaacg gttaatcacc cacttggtgg tcgttaccaa ctcacaccaa ctgaggcatc
     7081 tgtgcagaaa ttgccagttc aacacggtgt gactcatact gcgtcggtca ttgctcaagg
     7141 tttcaaccca tatgtagctg aatggtctcc ataccacggt gctgcttacg cggttatcga
     7201 agcaactgct cgtttggtgg ctgctggtgc caactggtct aaggctcgtt tctcttacca
     7261 agaatatttc gagcggatgg acaagcaagc agagcgtttt ggtcagccag tagccgccct
     7321 tctaggctct attgaagctc agattcaact tggcttgcca tctatcggtg gtaaggactc
     7381 catgtctggt acctttgaag aattgaccgt tccgccaacc ttggttgcct ttggggtgac
     7441 gacggcagat agccgtaagg tgctctctcc agaatttaaa gctgttgggg aaaatatcta
     7501 ctacatccca ggtcaagccc tctctgcaga gattgatttt gacttgatta agaaaaattt
     7561 tgctcagttt gaagcaagcc aagctgacca taaagtgaca tctgcatcag ctgtcaaata
     7621 cggtggtgta gttgaaagtt tggctcttgc tacctttgga aactatattg gtgcagaggt
     7681 gaccttgcct gaacttaaaa cagctttgac agctcaatta ggcggctttg tcttcacatc
     7741 tcctgaagaa attgctggag tagagaagat tggacaaacg aaagcagact ttacactgac
     7801 tgtcaacggt gtgaagctag atggacacaa gcttgacagt gcatttcaag ggacattgga
     7861 agaagtttac ccaacagaat ttacccaagc gaaagaacta gaagaagtac cagctgtggc
     7921 atcagatgtt gtgattaaag ccaaagaaaa ggttgaaaaa cctgtggttt acatcccagt
     7981 ctttccagga accaactcag aatatgattc agctaaggcc ttcgaaaaag aaggtgcaga
     8041 ggtcaatttg gtgccattcg tgaccttgaa tgaagaagct attgtcaagt cagttgaaac
     8101 tatggttgac aatatcgaca agactaatat tctcttcttt gccggtggat tctcggctgc
     8161 ggatgaacca gatggttcag ctaagtttat cgtcaatatc ctgcttaatg aaaaagtgcg
     8221 tgtggctatt gatagcttta tcgcccgtgg tggcttgatt atcggtattt gtaatggatt
     8281 ccaagcctta gtcaaatcgg gtctcctacc atacggaaac tttgaagatg ctaacagtac
     8341 tagtccaacc ctcttctaca atgatgccaa ccaacacgtg gccaagatgg tggaaactcg
     8401 cattgccaat accaactcac catggttggc tggtgtgcaa gtgggcgata tccacgctat
     8461 tcctgtttcg cacggtgaag ggaagtttgt cgtgacggct gaggaatttg cagagctccg
     8521 tgacaatgga caaattttca gccaatacgt tgactttaac ggtaaaccaa gtatggattc
     8581 taagtacaat ccgaatggtt ctgtccatgc catcgaagga attaccagca agaatggtca
     8641 aatcatcggt aagatgggcc actcagaacg ttatgaggat ggtcttttcc aaaatatccc
     8701 aggcaataaa gaccaacacc tgttcgcatc agcggttaaa catttcactg gaaaataaga
     8761 cttacagatt ttctaataga tagtatcagt aatgtaaaag tcatgtaaat ctagctcttg
     8821 atgattacaa atgaaaatta ggtataaaaa atgacatacg aagtaaaatc tcttaatgaa
     8881 gaatgtggtg ttttcggtat ttggggacat ccagatgctg ctaagttgac ctattttgga
     8941 ctccacagtc ttcaacaccg tggtcaggag ggggcaggaa tcctctccaa cgatcaagga
     9001 caactgaagc gccatcgtga catggggctt ttatcagaag ttttcagaaa tccagctaat
     9061 ttggataaat tgacaggagc tggtgcgatt gggcatgtgc gttatgcgac tgctggcgaa
     9121 gcttctgtag ataacatcca gcccttcctc ttccgttttc acgatatgca gtttggcttg
     9181 gctcataatg gaaatctgac caatacagcc tctctcaaga aagaactgga acaaagagga
     9241 gcaattttca gcgcgacttc ggactctgaa atcttggctc acctcattcg tcgcagtcat
     9301 aatcctagcc tgatgggcaa aatcaaggaa gcgctcagcc ttgtcaaagg tggttttgcc
     9361 tatatcttgc tgtttgagga caagttgatt gcggctcttg accctaatgg ctttcgtccg
     9421 ctttctatcg ggaaaatggc caacggagcg gtggttgttt cctctgaaac ctgtgctttt
     9481 gaggtcattg gtgccgagtg gattcgtgat ttgaagccag gtgagattgt gatcattgat
     9541 gacgagggca ttcagtatga cagctataca gatgataccc agttggcgat ttgttctatg
     9601 gagtatatct actttgcccg ccctgattct aatatccacg gtgtcaatgt ccatacggca
     9661 cgtaagagaa tgggagcgca attggcgcga gaatttaagc atgaggcaga tattgtagtt
     9721 ggtgtgccca attcttccct aagcgcggct atgggatttg cggaagaatc aggcttacca
     9781 aatgaaatgg gtctgatcaa aaaccaatac acccagcgaa cttttatcca accgactcaa
     9841 gaattgcggg agcaaggagt gcggatgaaa ctgtctgctg tttcgggtgt tgtcaaaggc
     9901 aaacgtgtgg tcatggtgga tgattccatt gtacgtggaa caacctctcg tcgtatcgtt
     9961 cagctcttga aagaagcggg tgcgactgag gttcacgttg ccattggaag tcctgcacta
    10021 gcgtatccat gtttctacgg gattgatatc cagacccgtc aggagctgat tgcagccaat
    10081 catacggtcg aagaaactcg ccaaatcatt ggtgcggaca gtctgactta tctttcaatt
    10141 gatggcttga ttgagtcgat tggtatcgaa acagatgcgc cgaacggtgg tctctgtgtc
    10201 gcttactttg acggtgacta cccaacgcct ctttatgact acgaagaaga ctatcgtaga
    10261 agtttggaag aaaagaccag tttttacaag taggcgacag attctccatt aaagaaaagg
    10321 aaaaaacaaa tgacaaataa aaatgcatat gcccagtcgg gtgtggatgt tgaagcgggt
    10381 tatgaagttg ttgaacggat taaaaagcac gtggcccgta cggagcgtgc aggtgtcatg
    10441 ggagctcttg gtggctttgg tggtatgttt gacctttcca agactggggt taaagaaccc
    10501 gtcttgattt cagggactga cggtgtcgga accaagctca tgttggctat caagtacgac
    10561 aagcacgata ccatcgggca ggactgtgtg gccatgtgtg tcaacgacat cattgctgca
    10621 ggtgcggaac ccctctattt tctcgactac gtagcgacag ggaagaatga accagctaag
    10681 ctagaacaag tggttgctgg tgtggcagaa ggttgtgtgc aggctggtgc tgccctcatc
    10741 ggtggggaaa cggctgaaat gccgggcatg tacggcgaag acgactatga cttggctggt
    10801 tttgcggtcg gtgtggctga aaaatctcaa atcattgacg gttcaaaggt ggtagaggga
    10861 gatgttcttc tcggacttgc ttcaagtggg attcactcaa atggttactc tttggttcgt
    10921 cgtgtctttg cggattacac aggtgaggaa gtcctaccag aattggaagg caagaaactt
    10981 aaggaagttc tacttgagcc gactcgtatc tatgtcaagg ctgtcttgcc gctcatcaaa
    11041 gaagagttgg tcaacggcat tgcccacatc acaggtggtg gttttatcga aaatgtccct
    11101 cgtatgtttg cagatgacct agctgctgaa attgatgaaa gtaaagttcc agtgcttcca
    11161 attttcaaaa cccttgaaaa atacggtcag attaaacacg aagaaatgtt tgaaatcttc
    11221 aatatgggtg tgggactcat gttggcggtc agccctgaaa atgtagagcg tgtaaaagaa
    11281 ttgttggatg aagcagtcta tgaaattggt cgcatcgtca agaaagaaaa cgaaagtgtc
    11341 attatcaaat gaaaaaaata gcggtttttg cctctggtaa tggctcaaat tttcaggtga
    11401 ttgccgaaga atttccagtg gagtttgtct tttcagacca tcgtgatgcc tatgtgcttg
    11461 agcgtgcaaa gcagctcggc gttctgtcct atgcttttga actcaaggag tttgagagca
    11521 aggcagacta cgaagcagcc cttgtcgaac tcttggaaga acaccagatt gacttggttt
    11581 gcctagcagg ctacatgaaa atcgttggac caaccttatt gtcggcttat gaaggtcgga
    11641 ttgtcaacat tcatccagcc tacttgccag aatttccagg agctcatggg attgaggatg
    11701 cttggaatgc tggcgtgggt cagtctggtg tgaccattca ctgggtggat tcgggtgtgg
    11761 atacaggcca ggtcatcaaa caggttcgtg tgccacgact agctgatgat accattgaca
    11821 gatttgaagc tcgcatccat gaagcagagt acaggttgta tccggaagta gtgaaggctc
    11881 tatttacaga ttgacttttt gatgattcat atgatatctt tgattttaaa ttggagtcag
    11941 tgtttgttga agacggcttc aaacggaggt atttgtaatg ttagaatcta aaaaaacaac
    12001 tcgatatgta ttttatgtct atctgatgtt attaacttgg ggaatcttat ttaagtttga
    12061 aacaaatcct gaatttatag catttttctt agctccaagg tatatcaatt ggattccatt
    12121 ttcagaacca ctaatagtcg atggaaaaat tgtttttgct gaaatgttat ttaatctgat
    12181 tttctttatt ccattaggtg tttgtttccc tttgataaaa actaatttat ctagtttaag
    12241 aatagtcggg acaggtttct tgattagttt attgtttgag tacttacagt atattttagc
    12301 aataggtata acagatataa cggatttgac tttaaatacg ctaggtgtct gtgtaggctt
    12361 actgatttat caaattttta taagagtgtt caaatcacag actagaaaat ggatcaatat
    12421 cttaggtatg cttagccttg gttttgctta tcttgtttta ctgttactgc atttaattgg
    12481 tgtttaacta atgattaaaa aggagaatat aatgactaaa cgcgtcttaa tcagcgtctc
    12541 agacaaagcg ggcattgttg aatttgccca agaactcaaa aaacttggtt gggagattat
    12601 ctcaacaggt ggaactaagg ttgcccttga taatgctggg gtggatacca ttgctatcga
    12661 tgatgtaact ggtttcccag aaatgatgga cggtcgtgtg aagaccctcc acccaaatat
    12721 ccacggaggg cttctcgctc gtcgtgactt ggatagccac ttggaagcgg ctaaggacaa
    12781 caagattgag ctcattgacc ttgtggtggt caacctttac ccatttaagg aaactatcct
    12841 taaaccagat gtgacttatg ctgatgcagt tgaaaatatc gatattggtg ggccgtctat
    12901 gcttcgttca gcagcgaaaa atcatgccag tgttacagtt gtggtagatc ctgctgacta
    12961 cgctgtggtt ttggacgaat tggcagcaaa cggcgaaacc tcttatgaaa ctcgccaacg
    13021 tttagcagcc aaagtatttc gtcacacagc ggcttatgac gccttgattg cagaatactt
    13081 cacagctcaa gtgggtgaaa gcaagcctga aaaactcact ttgacttatg acctcaagca
    13141 accaatgcgt tacggtgaga atcctcaaca agacgcggac ttttaccaga aagctttgcc
    13201 tacagattac tctattgctt cagccaaaca gctcaacggg aaagaattgt catttaataa
    13261 tatccgtgat gcagatgctg ctatccgtat catccgtgac ttcaaagata gtccaaccgt
    13321 tgtggctctc aaacacatga atccatgtgg aattggtcaa gctgatgaca tcgagactgc
    13381 ttgggactac gcttatgagt ctgacccagt atctatcttt ggtgggattg tcgtcctcaa
    13441 ccgtgaggtg gatgctgcga cagctgagaa gatgcacggc gttttcctcg aaatcatcat
    13501 tgcaccaagc tatacggatg aagcgctagc cattttgatc aataaaaaga aaaacttgcg
    13561 tatccttgcc ttgccattta atgctcaaga ggctagcgaa gtggaagcag aatacacagg
    13621 tgtagtcggt ggacttctcg tgcaaaatca agacgtggtc aaggaaagcc cagctgactg
    13681 gcaagtggtg accaaacgtc agccaactga gacagaagcg actgctcttg agttcgcttg
    13741 gaaggctatc aagtacgtca aatcaaatgg tattatcgtg accaacgacc acatgacact
    13801 tggtgttggt ccaggtcaaa ccaaccgtgt ggcttctgtt cgccttgcca ttgaccaagc
    13861 caaagatcgt ctgaacgggg cggtccttgg ttcagatgcc ttcttcccat ttgcggataa
    13921 cgtggaagaa atcgccaaag caggaattaa ggccatcatc cagcccggtg gctctgtccg
    13981 tgaccaagaa tccatcgaag cagcggataa atacggcttg actatggtct ttacaggtgt
    14041 gagacatttt agacattaag aagataaaag ggaagaaaac agtttctttc cttttttggc
    14101 ttaaaatgct aactgaaaca agattaaaac gaactttttt gatataatgt tggtaaataa
    14161 ttcgcaaaag aggttgagga atgaaactgc ttgttgtcgg ttctggtggt cgtgagcatg
    14221 cgattgctaa aaagttactt gaatcaaaag acgtggaaaa agtctttgta gctcctggga
    14281 atgacggtat gactctggat ggtctggaat tggtaaatat atctatttcc gaacattata
    14341 aattgattga cttcgcaaag gccaacgata ttgcttggac ctttatcggt ccagatgacg
    14401 cccttgcggc tggtatcgtg gatgatttcc atgcagctgg tctcaaagcc tttggtccga
    14461 caagattggc agcggagctg gagtggtcca aggatttcgc caaggaaatc atggtcaaat
    14521 atggtgtttc gacagcagcc tatggcacat tttcagattt cgaggaagca aaggcctata
    14581 tcgaaaagca tggtgcgcct atcgtagtca aggcggatgg cttggcgctt gggaagggtg
    14641 tcgtcgttgc tgagacggtt gagcaagcgg tcgaagccgc tcatgagatg cttttggaca
    14701 ataaatttgg tgactcaggt gcgcgcgtgg ttattgagga attccttgaa ggagaggaat
    14761 tttcactctt tgcctttgtc aatggtgata agttctacat catgccaacg gctcaggacc
    14821 acaaacgtgc ctacgatggc gacaaagggc ctaacacggg tggtatgggt gcctatgcgc
    14881 cagttccaca cttaccacag agtgtagttg atacagcggc tgacaccatt gtcaagccag
    14941 ttctagaagg gatgattaaa gaaggtcgcc cttatctggg agttctttac gcagggctta
    15001 tcctgacagc tgatggaccg aaagtcattg agttcaacgc tcggttcgga gatccagaaa
    15061 ctcagattat cttgcctcgc ttgacctctg actttgctca aaatatcaca gatatcctgg
    15121 atagcaagga gccaaatatc atgtggacgg acaagggtgt gactctgggt gtggttgtcg
    15181 catccaaggg ctacccgcta gactatgaaa ggggcgttga gttgccagcc aagacagaag
    15241 gcgatgtcat cacctactat gcaggggcta agtttgcgga aaatagcaga gcactgctct
    15301 caaacggcgg acgagtttat atgctcgtta ccacagcaga taccgtcaaa gaagcccaag
    15361 ccagcatcta ccaagaacta taccaacaaa aaatagaagg actcttctac cgaacagata
    15421 tcggaagcaa ggcaattaag taaagatata agaataacgc gccgtagtcg ccaaacacga
    15481 taatggtcgt cgtggtgaaa agaccagaac agtgaatgtt ctggtcaggg gaaacttgga
    15541 gaccttaggc tcaaagttta ggaatgaaac cgaaggtttg cttccgcctc catcacctaa
    15601 gaccattatc aaaaagaaaa ataaaaattc acaaaatacg ttaatgatcg tatggtttgc
    15661 gagcgttagc gagctaatat agaacaatca ccgccgttgt gaaagaacga ttggatgata
    15721 atccaatcgt tcagggaaat tggaagacct tgggtttcca atttaggcat gagacacctt
    15781 tggtggctgc tgccgtccct cacaagctaa ggtgattgtt gaaaaagagg aaaaaggaga
    15841 agaaatgaaa ccagtaattt ccatcatcat gggctcaaaa tccgactggg caaccatgca
    15901 aaaaacagca gaagttctag accgcttcgg tgtagcctac gaaaagaaag ttgtttccgc
    15961 acaccgtaca ccagacctca tgttcaaaca tgcagaagaa gcatatagcc gtggtattaa
    16021 ggtcatcatc gcaggtgctg gaggcgcagc ccacttgcca ggaatggtag ctgccaaaac
    16081 aacccttcct gtcatagggg tacctgtcaa gtctcgtgct cttagtggag tggattcact
    16141 ctattctatc gttcagatgc cgggtggggt gcctgttgcg accatggcta tcggtgaagc
    16201 tggagcgact aacgcagctc tctttgccct ccgtctcctc tctgtagaag ataagtccat
    16261 tgcggatgca cttgccaact ttgctgaaga acaaggaaaa atcgcagagg agtcgtcaaa
    16321 tgagctcatc taaaacaatc ggaattatcg gtggcggtca actgggtcag atgatggcca
    16381 tttctgctat ctacatgggc cacaaggtta tcgcgctgga tcctgcggcg gattgcccgg
    16441 tctctcgtgt ggcggaaatc attgtggcac cttataacga tgtagacgcc ctccgtcagt
    16501 tggcagaccg ttgcgatgtc ctcacttacg agtttgaaaa tgtcgacgct gacggtttgg
    16561 atgccgttat caaggatgga caactccctc aagggacaga cctgctccgc atttcgcaaa
    16621 atcgtatttt tgaaaaggac tttttgtcaa acaaggctca agtcactgtg gcaccctaca
    16681 aggtcgtgac ttctagccta gacttggcag atatcgactt gtcgaaaaac tatgtcctca
    16741 agactgcgac tggtggctac gatggtcatg gacaaaaggt tattcgttca gaagtagact
    16801 tggaagcagc ctatgcgcta gcagaatcag cagactgcgt cttggaagaa tttgtcaact
    16861 ttgcccttga gatttctgtc atcgtgtcag gaaatggcaa ggaggtgacg tttttcccag
    16921 ttcaggaaaa tatccaccgc aacaatatcc tgtctaagac catcgtacca gcccgcattt
    16981 ctgaaagtct agtagacaag gctaaagcta tggcagtgcg aatcgcagaa caactcaact
    17041 tgtctggaac tctctgtgtg gaaatgtttg cgactgatga tgacgttatt gtcaatgaaa
    17101 tcgccccacg accacataac tctgggcact attctattga agcctgtgat ttctctcagt
    17161 ttgacaccca tattctcggt gttctcggag caccattacc agtcatcaaa ctccatgcgc
    17221 cagccgttat gctcaatgtc ctaggtcaac acgtcgaggc tgctgaaaag tatgtcgcag
    17281 aaaatccaag cgcccacctc cacatgtatg gtaaaataga agcaaagcat aatcgtaaga
    17341 tgggacatgt gaccttgttt agtgatgtac cggatagtgt ggatgagctt ggggaaggga
    17401 ttgattttta ggacaagtct atgatacaaa ttatcgttaa tacatttatt gaaaaggata
    17461 agactggagc agttgttgaa gtgttgtatg ccagtgctga ccaagataag gtacaagcta
    17521 aatatgaaga actagctgca caataccccg aaaattattt agctatctat gatgtaccgc
    17581 tggatacgga tttgaataca ctagatcatt acccgtctgt gtttattgaa aaagaggagt
    17641 ttgagtagaa atcttggttt acctagatag cttattccca acagcttaag aagaaaggaa
    17701 aaattaacac atgatcaacc gttactctcg ccctgagatg gcgaatattt ggagtgaaga
    17761 aaataaatac cgtgcttggc ttgaggtgga aatcctctct gacgaggcat gggctgagtt
    17821 gggggaaatc cctaaggaag atgtggcttt gattcgcaag aaggcggact ttgacatcga
    17881 ccgtattttg gaaattgagc aggagacgcg ccacgatgtg gtggctttca cgcgtgcggt
    17941 ttctgagact cttggtgaag agcgcaagtg ggttcactat gggttaactt ctaccgacgt
    18001 ggtggatact gcttatggtt acctctacaa gcaggccaac gacatcatcc gtcgtgacct
    18061 tgaaaacttc actaatatca tcgctgacaa ggccaaggag cacaagttca ccatcatgat
    18121 ggggcgtact catggtgtgc acgctgagcc gacaaccttt ggtcttaaat tggcaacttg
    18181 gtacagcgaa atgaaacgca atatcgagcg tttcgagcat gcagctgctg gtgtagaagc
    18241 tggtaagatt tctggtgcgg ttgggaactt tgccaatatc ccaccatttg tagaggagta
    18301 tgtctgcgat aaacttggca tccgtgccca agaaatctct acacaagtcc ttcctcgtga
    18361 ccttcacgct gagtactttg cggttcttgc cagcattgcg acttcaatcg aacgtatggc
    18421 gactgagatt cgtggtctac aaaaatctga gcaacgcgaa gtagaagagt tctttgctaa
    18481 agggcaaaaa gggtcttcag caatgcctca caaacgcaac ccaatcggtt ctgaaaatat
    18541 gactggtctg gcgcgtgtca ttcgtggtca catgattacg gcttatgaaa acgtcgctct
    18601 ctggcacgaa cgcgatattt ctcactcatc agctgagcgt atcatcacac cagatacgac
    18661 cattttgatt gactacatgc tcaaccgttt tggaaatatc gtcaagaact tgacagtctt
    18721 cccagaaaat atgatccgaa acatgaactc gacttttggt cttatcttta gccaacgggc
    18781 tatgttgaca ttgattgaaa aaggcatgac ccgtgagcaa gcctatgact tggtgcaacc
    18841 aaaaacagcc tactcttggg acaaccaagt agactttaaa ccacttcttg aggcagattc
    18901 agaagtaaca tcacgtctca ctcaagaaga aatcgatgaa atcttcaacc cagtttatta
    18961 caccaaacga gtggatgata tctttgaacg tcttggacta ggtgattaat taaaaaataa
    19021 acagcgagct tcaatctcgc tgtttatttt ttatcgaaaa gacttagtct tcttttcttt
    19081 tagtgagtcc ataggctgct agtgtggaca tgagtcctgc gactactagt cctgcagaat
    19141 cgtgagttcc tgtttcagga agttttttct ctgttaccac aggagctgga tcttgaggaa
    19201 gagctttgct ttcctcagca ggagcagttg atggagctgg ttggcttggg atttctagtt
    19261 ttggtttttc ttcagcaata gcggcttgtc cgttttcatc gcctacatgt gttaccatag
    19321 ttccgacttc gactatttga gtaacggctt cctgtgctac gacactattt acaagtgttt
    19381 tcacttcctt accatcggca gaagtgctca cagagtagaa gttgctacga tgtccattga
    19441 cgcccttagt aatgacttgt gtttttcctt tgagtaagag tggattttca cgagtcactg
    19501 tggtaaatgg aatttcttct tcttggatat ccagtctagg ttttacctca gtagttggtg
    19561 caagaccact ttcatcaccc ttgtgagtta caggagagcc aacttcaacc acttggttta
    19621 taacttcttt ggttacctgg ctatcaagga ctgtttctgt tgtttttcca ttttcagtga
    19681 gtacagagat gtaatgagtt cgttcacctt tgactcctgc tgtgataata ttttcctgac
    19741 cagctgggag gttaggattt tctttcttga taacttcaaa tggaatttct tcagttcttg
    19801 tgatgagttc tggtctggtt tcaacattgg cagccacttc attttcatct aggcttcctg
    19861 aatgagttgc agctggtttg aggccttgaa gagcggcttt taggttggct acaagtgtgt
    19921 caagctcagc ttgtttatta cggttgaggt tgtaatttag agctgtttta gctgcgtcaa
    19981 gggcctcaag actttcttta ctatatcctt ctaagtttgt aggaatttta gctaattctt
    20041 cgcggagagc attataatta gcacggaagt agtctttgtt gtggtctgca aaggcagtca
    20101 tgagttcaaa gatttcctct tccttgtact cagcgcttgg tctatctgcc cagattgaaa
    20161 gcatacttcc gactgttgga agatctactt caggatattt ggtagaagct agttgattga
    20221 atggaacttt accagaattt tcaactgctt ttgaaagagg gtaagcttca tcttgtttgt
    20281 gattgcctat aacataatac cagtcaccgt tggtattcaa gaatttatag cctttgcttg
    20341 ctaggtattg aggtgatgcg aggttatatc cccaccagcc tttagaccag taagaaatca
    20401 agacatcttt gtcaaactga acatcgtcct tgtcttcata gtagaagcca tcgttgaagg
    20461 ccattggttg aagccctctt tctttggcca tagctgcgag ggtgttggca tattcggcaa
    20521 atttgccata gagttgatac cacttgaggt agtaccagcc ttgggcacta gtcgcatcgt
    20581 tggcgtattc gtcagtacca aagttgaaaa tctttgtttt acctgcaaag aagtccatgt
    20641 atttaccgat gagggctttt acaaagttca tcgcttcttc gtttttcaag tccatagttg
    20701 tttttgaaac tttatcaaag tgggcttgag gatttttaat acctaatttt tccatggcaa
    20761 ccagcatagc atccatgtga cctggactgt taatagctgg gatgagaccg atgtccttag
    20821 atttagcgta ttcaattagc tctgttactt ctgcctgtgt tagtgcagta ccgtttggat
    20881 cgtcgtagta agctttagtt ccttcgataa tagctttttt aacgtcatca ctagcatagg
    20941 tttttccgtt ggcagtaatg gtcatatcat cgagtagaaa gcgaagtccg tcatttccta
    21001 gaaggagatg gacatcagaa tatccgagct cactggcctt gtctacgatg cgtttgagct
    21061 ggttcagagt aaagtatttg cgtccagcat cgattgagat taccttgttt ttggcaagtt
    21121 tttcaacctc acgtttagct tcttcttctt tttgagcttc aggcgtgagg gtcaagttgt
    21181 tgacagtttc ttgaagttta gcaatggctt gatcaatcgt atcttgttgg gcacggctaa
    21241 ggttgctatc gagagagcga atagcttttt cagcttcttt tacggccgtg acgctttctg
    21301 cagtataacg gttcaggtct tttggtacct cgttaagtgc ttgctctgca gattcataat
    21361 cagctgcgaa gtattcagcg ttggcatttg caaaatgacg catgagtttg aagaggcgtg
    21421 atggtgaata acgtgcagat ggagtgtcag cccaagcagc taccatacca ccgatgattg
    21481 ggatatcagc tccttctgtt tttggtacag aagtgattgg tgtgttttta ataccattga
    21541 gcccctgatc gagattgtac cagccttggc catcagcgtt tcgtccaaga acgtagtacc
    21601 aagcatcatt ggtattaagg atttggtgac ctttttcagc tagtagttta gaagaagcga
    21661 catcgtagcc tccccaacca ccagtccaca tagaaacgat gatgtctttg tcaaaactac
    21721 caaagcttgt gtcgctattg tagtagatac cgtcgttaaa ggccattggt ttgagaccgt
    21781 gcgattttac aatacgagcg aggtcattgg cgtaggcaat aaatttttca tagcctttta
    21841 cagggtagcc ttcgtttgga tagtatttat cagcttgaag cacactccaa cctttagcat
    21901 ctgtcgcatc attggcatat tcatcaagtc cgatgttgaa gatttcagtc tttttcgcga
    21961 aataagcagc atacttgtcg ataagggctt ttgtaaaagc gacagcttgt tcgttgtcaa
    22021 gatcgacagt acgggctgat ttcttcccaa aatagctaaa gttagggttt tggattccca
    22081 attctttcat ggcattgaga atcgcatcca tgtgtccagg actatttact gtcggaatga
    22141 gaccgatacc tttatctttg gcatagttaa tcagatctgt catttgactt tctgttaagt
    22201 gattgccgtt tggatcgttg taataatcat ttgtaccttt ttcaatggcg cgtttgacat
    22261 cgtcactggc ataggtcttg ccgttagctg tgatgctcat atcgtccaac atgaaacgga
    22321 gtccatcatt tccgactaat aggtgtaaat cagtgtagcc ataatgtttc gctttatcga
    22381 tgatttcctt gagctgttct ggtgagaaat atttacgtcc agcatcaata gaaacaattt
    22441 tctttttcgc tagtttttca tttacagttg cagcacgttc ctttcctgcc tctgtttccg
    22501 gtttgtcagc ctctgctttc gcttcatctt ttttagctgg tttatccttg tcagtcttgt
    22561 ctgtatttga ctctttagaa tcaacctctt tcgcttcttc ctttttaggg ctagcttctt
    22621 ctgccttttt attagcagtt tctttttcag cagaagttgg agttaccact tctgctttat
    22681 cactaggagt tgaactaact tcctcttgtg gtttttcttc tgtttttgga agactagcta
    22741 ccttatcagt agctggagtt tctgtttcta cagtttttgg agcttctggt tgaagctctg
    22801 ctttaggtgt ttcctcagtc cgattttcgg atgattgagg ggaatcagaa accgtatgga
    22861 tagtcggttg gttttctgta gtagtaggag taactccatc ggctgcaaca gtctgtgctt
    22921 ggaaggcaaa tccaattaga acagaagctg ctcctacagc gtatttacga atagaaaaac
    22981 gctgttgttt ttcatgtttc attgcaaaac ctcctgattg cattgttata ttgatagcga
    23041 ttatataaat caacgccttt attttatttc ttatattaac gagagtcaag aggagatgac
    23101 aaaaaactat aataagtata aaaaaatata aaatttaaac ttaagatttc agattggtcg
    23161 aaaaaataca tatatatatc tagtataatt tttggttcta tttctataaa atattccaca
    23221 aattatagaa tcttccaaaa ataggtaagc gctacctttt tggtgtggta taataagcat
    23281 agaaaaagcc cgagcgatta ggctcaggct ttcttcttag tgatcacggt cacatgagat
    23341 gaatttaatc ttgtagtaat cagatcgttt gtaagtttca ctgtattcta aaacttggcc
    23401 agttgattcg agtttggtga ttttagtttg taggacagta gggaattgtt catcgactcc
    23461 gaggactgaa gccgcatgtt ctggagttgg aaagactatt tcgttgattt cttcaaagtg
    23521 ttcatcattc atgtgaatgt ggtagtctaa cttgaaacga ttatagatag aactatagta
    23581 ttcaaggttt ggataatttg cgttgatata ttgttctggg atgtaggatg tatggtagat
    23641 ataaacgaca ccgtttgatt cgcggatacg ttcaatcttg tagtagaatt gatcgccgcg
    23701 tagacccaat ttttccaagt aaacaagctt gtttccgcgt tcaattgaaa gaacagttac
    23761 cttatcatct ttagcattga agagttcaat atctgaaaac tctacaagct tgtgtttgcg
    23821 tgcacgtgaa acgaaggttc cttttccttg ttggcggaca atatagccat ctttggcaag
    23881 gtcgtttaag gcgcgaacaa ctgtgataga gctgacatcg tacattgaaa tgagttctgc
    23941 ttcagtgtaa aatttatctc cactgctaaa ctgcccagag atgattttat tttttaattc
    24001 gtcttttatg tattgatatt tgggaattgc catattttta cctctatttt ccttctccat
    24061 agaaaatttt accataaaag cgaaaaaaat agtagtcttt tgaataaaaa attagaataa
    24121 tagtttgaaa aattaagcga tatagggatt gctcgta
//
LOCUS       DAVMHG010000017        22792 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMHG010000017 DAVMHG010000000
VERSION     DAVMHG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.017"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(11..922)
                     /gene="trxB"
                     /locus_tag="VYR08_000545"
     CDS             complement(11..922)
                     /gene="trxB"
                     /locus_tag="VYR08_000545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV3466120.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(988..1212)
                     /locus_tag="VYR08_000546"
     CDS             complement(988..1212)
                     /locus_tag="VYR08_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV3466121.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(1290..2033)
                     /locus_tag="VYR08_000547"
     CDS             complement(1290..2033)
                     /locus_tag="VYR08_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3466122.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(2033..2833)
                     /locus_tag="VYR08_000548"
     CDS             complement(2033..2833)
                     /locus_tag="VYR08_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3466123.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(2991..3347)
                     /locus_tag="VYR08_000549"
     CDS             complement(2991..3347)
                     /locus_tag="VYR08_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV3466124.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(3341..3871)
                     /locus_tag="VYR08_000550"
     CDS             complement(3341..3871)
                     /locus_tag="VYR08_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV3466125.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(3871..4365)
                     /locus_tag="VYR08_000551"
     CDS             complement(3871..4365)
                     /locus_tag="VYR08_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466126.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSSEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            4500..4946
                     /locus_tag="VYR08_000552"
     CDS             4500..4946
                     /locus_tag="VYR08_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV3466127.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            4943..5590
                     /locus_tag="VYR08_000553"
     CDS             4943..5590
                     /locus_tag="VYR08_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV3466128.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(5611..6192)
                     /gene="pdxT"
                     /locus_tag="VYR08_000554"
     CDS             complement(5611..6192)
                     /gene="pdxT"
                     /locus_tag="VYR08_000554"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV3466129.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(6193..7068)
                     /gene="pdxS"
                     /locus_tag="VYR08_000555"
     CDS             complement(6193..7068)
                     /gene="pdxS"
                     /locus_tag="VYR08_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV3466130.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(7220..8599)
                     /locus_tag="VYR08_000556"
     CDS             complement(7220..8599)
                     /locus_tag="VYR08_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV3466131.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            8894..9817
                     /locus_tag="VYR08_000557"
     CDS             8894..9817
                     /locus_tag="VYR08_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV3466132.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIKLDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            9877..10482
                     /locus_tag="VYR08_000558"
     CDS             9877..10482
                     /locus_tag="VYR08_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3466133.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            10501..11745
                     /locus_tag="VYR08_000559"
     CDS             10501..11745
                     /locus_tag="VYR08_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3466134.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(11968..12225)
                     /locus_tag="VYR08_000560"
     CDS             complement(11968..12225)
                     /locus_tag="VYR08_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV3466135.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(12267..14303)
                     /locus_tag="VYR08_000561"
     CDS             complement(12267..14303)
                     /locus_tag="VYR08_000561"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV3466136.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFRGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKKIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(14515..15432)
                     /gene="glyQ"
                     /locus_tag="VYR08_000562"
     CDS             complement(14515..15432)
                     /gene="glyQ"
                     /locus_tag="VYR08_000562"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV3466137.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(15803..16563)
                     /locus_tag="VYR08_000563"
                     /pseudo
     CDS             complement(15803..16563)
                     /locus_tag="VYR08_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(16836..17678)
                     /locus_tag="VYR08_000564"
     CDS             complement(16836..17678)
                     /locus_tag="VYR08_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV3466138.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(17792..19183)
                     /locus_tag="VYR08_000565"
     CDS             complement(17792..19183)
                     /locus_tag="VYR08_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV3466139.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(19418..19795)
                     /locus_tag="VYR08_000566"
                     /pseudo
     CDS             complement(19418..19795)
                     /locus_tag="VYR08_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            20010..20987
                     /locus_tag="VYR08_000567"
     CDS             20010..20987
                     /locus_tag="VYR08_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3466140.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            20984..22066
                     /locus_tag="VYR08_000568"
     CDS             20984..22066
                     /locus_tag="VYR08_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3466141.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(22130..22684)
                     /locus_tag="VYR08_000569"
                     /pseudo
     CDS             complement(22130..22684)
                     /locus_tag="VYR08_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(22718..>22792)
                     /locus_tag="VYR08_000570"
     CDS             complement(22718..>22792)
                     /locus_tag="VYR08_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV3466142.1"
                     /translation="YNNKKIKIKLKGLSPVQYRTKSFG"
BASE COUNT         6681 a         5123 c         4093 g         6895 t
ORIGIN      
        1 ttttttatga ttaactatgt tctgtaatga atttataggc ttcttgacca gcgatagctc
       61 catctccaac cgctgttgtt acttggcgaa ggtctttcaa gcgaacatct ccaactgcaa
      121 agataccgtc gactgcagtt ttcatgtggt tatctgtcac aatccatcct gcctgatctt
      181 ggatattcaa ttctttaaca aaatcgctaa gagggtccaa accaacatag ataaagacac
      241 caccgaaggc ttgttctgtc acttgacctg ttttcacatt ttcaaatacg actgattcta
      301 ctcggttttc acccttgatt tcccttacta cagaatccca gataaagctg attttttcat
      361 tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag ttggtcacga cggtgaacaa
      421 tggtaacagt cttagcaaaa cgagtcaaga agagggcttc ttcaacagct gaatctccac
      481 caccaactac caataaatct tggtcacgga agaaagcacc atcacacaca gcacagtaag
      541 aaacaccacg actgttcagt tcttcttctc caggcactcc caaaggacgg tgtttagaac
      601 cagttgctac gataactgta cgtgtttcat atgtttggtc atcagtcatc actttcttaa
      661 aatcaccatg gtcttcgaca ttttcaacat aaccataaat gtgctcaaca ccaagatttt
      721 caagtggttc aaacatcttt tcagccaatt caggtccact aatattagcg tatcctgggt
      781 aattttcgat atcagatgta ttattcatct gaccacctgg cagaccacct tcaatcaaag
      841 ctacttttag attgcttcga gcagcataca aggccgcagt catccctgca ggtccagcac
      901 cgataataat agtatcgtac atatagattc cttctttctt gttgtaacta tctttattct
      961 aactctttct tgtcaatcac gcaaggatta tgccttgaaa acaagaatta aactcataac
     1021 cgcaaaggat aatactggga caaccaagac tccaatcata atcatatcat agagatgggc
     1081 ttggcgagcc ctggctgtct tatcaactcc cgacatggct ctcagaccaa tccaaatccc
     1141 taaaaaaatc agaacaagga tggtggtcaa gatcaaactc tcgaaatata aagaaaatag
     1201 ttgcagtagc atgatttctc tcatttctat cttttttaaa gagtaaactc agctagtcca
     1261 actaactgag ttttccttta tctattatat caaatataag tccgtttgta actagcgaag
     1321 aattcttttg tccgctcttc tttaggggtg tggataatct catccggagt tccagactcg
     1381 atgattttcc ccttatctaa gaagagaatt ttatccgcaa cttgggctac aaagggcatg
     1441 tcatgactga ccaaaatcat ggtctgacct gacttagcag catctgcaat agacttttct
     1501 acttcaccga ccaattctgg gtcaagggct gaagttggtt cgtctaagag caaaacatct
     1561 ggtttcatag caagcgcacg cgctagggca acccgttgct tctgtccacc tgataaatgg
     1621 cgaggataat ggttttcacg gtccgaaagc ccaaccttag ccaactcttc cttggcaatc
     1681 ttagtcgctt cttggtcaga taatttcttg acaacaacca agccttcttt cacattatca
     1741 agtgctgttc ggcgttcaaa taaattaaac tgttggaaaa ccatagacaa cttacggcgt
     1801 agggcaagga tttcttcttg agtgatttta gaaaaatcaa ctgaaaaacc atcaatctga
     1861 atagagccac tgtcaggtgt ttcaagataa ttgagactgc gaagaaaggt tgattttcca
     1921 gctcctgaag aaccaatcaa ggctacaact tccccttttt gaatatccaa gttcagatga
     1981 tccaagacag tctgtcctga aaaggatttg cttaaattcg aaatcttaat cattaacgaa
     2041 ggtctccttt cacatctgtt tgcactgtat caggtgcaga aatagccatt tttctctcga
     2101 tgaaacgacc gaggctttca attccgatat tgactaccca ataaacaagg gcaacggaga
     2161 tgaagcgttc aaaatagcga taatcagctc cacctagaat ctgagcttgg gcaaagactt
     2221 ccacaacacc cgcactaaaa gctagagatg ttcccttggt caaaccgatg agggaattaa
     2281 tcaaggttgg agtagctacc accgctgcat taggaataat cactcgtcga taaacttgcg
     2341 ctcgggtcat acccagactg cgtgccgcct caatctcacc aggattaact gagagaatgg
     2401 ctgcacgaat ggtttcacta gcataagctg cctcattaaa ggcaaaagcg acaatcgcaa
     2461 aagctgcagc tggaatcgca ttgatattga gaccagttcc ccattgctga ttgagggctt
     2521 tcaaagccaa agggattccg tagtaggtca acatgagttg caccaaaatc ggtgtccctt
     2581 ttaagaaact aacaaagaag gcctgcaagg gatataaaat cttgacacga ttgatcttca
     2641 caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc accaccaatt gtcaacataa
     2701 ttgttgttgg aagttgttgg acaattctag ggattccatc aaagaccgaa cgtaggctaa
     2761 acagcttgcc atccggaatc aattgcatca agttttggta ccaatctgat gctaaaatcg
     2821 ttgtaacatt cataaaaaca tcctcttctg ataatgattc attctaccat gcttctgccg
     2881 tcaatgaaat tatttgctac ggtcagatac agactttgtc tacctactag tttatcatac
     2941 tttgaaacag ggaagttata tattttatct atcaaagaga tagataaaaa ctatttcagt
     3001 cccagttctt cataagattt tcgataaccg atttgcttaa cggttccatt ttctactaaa
     3061 atgggccgtt ttaacaacat accgtcactt gctagcaact cagccgcttc ttggtttgac
     3121 aaacttccta ccttatcttt tagccctaat tcacggtatt tgataccact ggtgttgaaa
     3181 aattgcttca attcaaatcc tgaggtttct agccaattca aaatgacttc ttggctaggt
     3241 gtttcttcca cgatatggac ggctttatag tccacaccta attgatttaa ttcttgtttt
     3301 gcttttttac aagttgaaca tttgggatat tcgataaatt ctaacatgtg cttctccttc
     3361 tattgtttct atctttaaaa tctactcctt catgctccaa gagccaagct ttcttttcca
     3421 ctcctgcagc ataacctgtc agacgcttgc ctgctcccaa cacacgatga caaggtacta
     3481 ggatagacca aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca
     3541 ggtcttgagc aatttgtcca taggtcactg tctgaccata aggaatgccc tgtaaatagt
     3601 cccaaactct cttttcaaaa ttcgttccaa ttggcgccaa gagcaagttg gataaatcct
     3661 gaggcttgcc tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac
     3721 taacaacttc ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa
     3781 cccaaattcc atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg
     3841 agtagaggat ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg
     3901 ttcgccatct actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg
     3961 cataaccttg ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat
     4021 caagccctct aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat
     4081 acggtgaaag actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc
     4141 ataggcctcc tctgaactct tggtcacagc agcataggct agcagttctc cctcttccaa
     4201 ggctacgtag gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc
     4261 attttgccac tggtcactac cagcatctgc taaacatttt ttggcatcct ccatcacctg
     4321 catgatggca tctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt
     4381 ctgactagtt tctcccatca tagcataatt taagtatttc tacaagcagt taaaacttga
     4441 ctttgtgttt ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa
     4501 tgaaaactac cttttcctac ccaaaatggg cagaaattcc aaacattgac ctctatctgg
     4561 accaggtttt gctctatgtc aatcaggtct gcgcccctat ctctcctaat aaagacaagg
     4621 gcctaacagc atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca
     4681 aaaaaaaata ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg
     4741 tattttctat tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag
     4801 accaactcta cgatgctttt gtggactaca tgaaccaagg aattgaccca gctaacccta
     4861 ttatccaaac cagctgccaa accgttaaac tctatcatca aactctagac ttaatcgatc
     4921 atactcaaga ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt
     4981 tggagaggag attgctaata gcgtgaccca tgctgtgggt gcagtcatca tgcttatctt
     5041 gctgcctatt tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt
     5101 ttccattttc gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat
     5161 ggcctatggt tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt
     5221 tgccattgcc ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta
     5281 tctgattatt gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc
     5341 taaaaaggtc aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct
     5401 ggctatcatt cctgccatta tcagtcaaac gacacccgtt ttctggagtc tcatggtaac
     5461 tggcggactc tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca
     5521 catgatttgg catctcttta tcctagctgc gtccgcactt caatacatcg ctattgttta
     5581 ttacatgtaa aaaagttgag aaattcaatc tcaacttttt tctttacaca tattgataaa
     5641 gtactggtgc aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt
     5701 tttttcttgg gctgcaacaa tttgattgtt cactgttgct agaatttcta caccctcacc
     5761 aacactactg ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca
     5821 ttctgcttcc gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat
     5881 atccatagtt cctagatgac tctctttctg agaagtgatt tccttagcca gcaaaattaa
     5941 gcccgcacag gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag
     6001 catgttctgg tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa
     6061 acccgacaag tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc
     6121 gacacctaat tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat
     6181 tccgattttc atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat
     6241 accaaccatg gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa
     6301 gttagtcaca gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat
     6361 acctgaaccg acaaagaccc cctctgcccc taattgcatc attaacgcag catctgctgg
     6421 cgttgcaaca cctccagcag cgaaatttac aactggcaat tttccatgtt catgaacata
     6481 ttggaccaat tctacaggga cttgcaaatc cttggcagca acataaagct cgtcctcacg
     6541 taagttttga atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac
     6601 gatatcccct gtccctggtt ctcctttggt acgaatcatg gaagcacctt cagcgatacg
     6661 acgcaaggct tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt
     6721 atccacatgg aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat
     6781 ttcaatagcc tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac
     6841 tggaatacta accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac
     6901 tcctccagct gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc
     6961 agcagcttct gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt
     7021 gagcatctgt gccaaatttt tatttagttc ataacgattt tcagtcattt gttttatcct
     7081 catcatttgt attggtagct accttttcat tttaagtcag aacagaatga agcacaagat
     7141 aaaaaaacta taattgtatg gggacaaatt agctaaaaac tatctgacct taacttaagg
     7201 ccagatagct cattcatttt tatttttcag ccgtaagggc agccattgtg atgtagttgt
     7261 atggtttgtt gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca
     7321 catgctcttg gatagcaagt gagaacatgt ggattcccat gctaattgca atatcatgtg
     7381 aaaccatttg ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta
     7441 cttcatggtt gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag
     7501 ttgcgttgta accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa
     7561 gaccgtagat tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac
     7621 aagcgttgta ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc
     7681 ttgtatcttt acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg
     7741 ggatagatgt ttcttgtttc ttgtctacaa ggaaggcacc gttgcggaag agttcgatct
     7801 taccatctgc aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt
     7861 caaagctttc tttgtcagta atcaagcgtt caactttacc gtcaccttcg attgctttaa
     7921 cagtttgacc tagagccaag cggatgttgt gatcttccaa gttcttcgcc atcatttgtg
     7981 tgaagtcttt gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca aggacaactt
     8041 cttttccaag acgttcaaag gcctcagcaa gttcaacacc gatgtaacca ccaccaacaa
     8101 cggcgatacg gtcgaggtgt tggctcttgt cagaaagttt attgataact tcttcagcat
     8161 tttggtacaa tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt
     8221 taacaatttc aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca
     8281 atttttcgta tgattctttg tgctcttttc cttcaacttc cgctgtaact actttgttat
     8341 catagtcgat tgaaagaaca ggtgagttca tgtaaacttt agcaccttta gcttccaatt
     8401 tttctttatc agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag
     8461 ccattccaca tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct
     8521 catttccaaa attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga
     8581 ctacaacgat tttactcata aaaaatttcc tgccttcatt ctaaatttac cttactagta
     8641 taccatttac cgttagcgtt tacaaggggg gtactatcat ttcagcattt tttatcaaaa
     8701 ataattcccc atttatcctg ttttcaagcc tgatgaattt tatgagcata tttcttgact
     8761 ttttccaaat tttgtttgtg aaaattctaa ctttatggta ttataataac atgaaaatga
     8821 aatgtaaggg cttcaattta gattattatt tgatattaca acttattttc tataagagaa
     8881 aggagttggt agcttgactc ttcgttcaca ttctgaacgg ctaatgggga ctactatcac
     8941 tatttcatta gtggatgagc aagccgatat ctttctccaa aaatcctttg acttgctcaa
     9001 agaacttgaa taccgcttca acgccaatag tcaagaatct gagttgatgg aaatcaatta
     9061 tcaagcagga atagcccccg tcacggttca tccagacctg tttgagctga tttcacttgg
     9121 gttagagcat agcctagcgc tctctagcca cctcaatatc agcattggtc ccttaattca
     9181 aacctggcgt atcggttttt cagatgccaa ggtcgcccag cctcaagaaa ttgaatcggt
     9241 gctgcctcta atcaatcctc atggtatcaa gttagattct tctacttcta ctgtgttttt
     9301 aaaacagaaa ggaatgaaga tcgatctagg ttgtttagcc aaaggataca gtgcggataa
     9361 ggttgcccaa tttcttagga aagagggggt gacttctgcc ttgatcaatc tgggagggaa
     9421 tatcctgacc attggaaaaa atcaggcaag aggggataac ccatggcaaa tcgggattca
     9481 agacccagcc aatcctaggg gaaatcattt aatgaccatc cctgttgtca ataaatctgt
     9541 cgtgacttca ggcatttatg aacgtcacct gaccgtcgat ggacaagatt accatcacat
     9601 ttttgacagt caaacaggat atcctgttga aacggaacta gcgagtctaa caatcatctc
     9661 tgataaatca gtcgatggcg aaatctggac gactcgactc tttggagaaa gacctgcttc
     9721 tatcctctgg caagtcgaaa gtttggaggg catcgaagtc atcctcatcg ataaagaagg
     9781 ccacctaagc tgttcttcag gaattcctac tctatagaga caactgtttc aatggaattt
     9841 taaaattgta ttatgtaaaa agagaaagga aatctcatgt taaaacttat tgctattgtt
     9901 ggaacaaatt caaaacgttc tacaaaccgt caattgcttc aatacatgca aaaacacttt
     9961 actgacaaag ctgaaattga gcttgttgaa atcaaggcca ttcctgtctt caacaaacca
    10021 gctgacaagc aagtacctgc tgaaatattg gaaattgctg ctaaaatcga agaggctgat
    10081 ggcgttatta tcggtactcc tgagtatgat cactctattc cagctgtttt gatgagcgct
    10141 cttgcttggt tgtcttatgg tatttaccca cttttgaaca aaccaatcat gattacaggt
    10201 gcttcttacg gtacgcttgg ttcatctcgt gcccaattgc aacttcgtca aatcttgaat
    10261 gctcctgaaa tcaaggcaaa tgttcttcca gatgaattct tgctctcaca ctctcttcaa
    10321 gcatttaacc caagtggcga cttggttgac cttgatgtta tcaagaaatt ggatgccatc
    10381 tttgatgact tccgtatctt tgtaaaaatc acagaaaaat tacgtaatgc acaagaatta
    10441 cttcgcaaag atgctgaaga ctttgactgg gaaaatttgt aagataggag accaaaaaga
    10501 atgaaatttg ttggacttgt tggatcaaac tacgatcaat catataaccg taagctcttg
    10561 gaatttatcc gtcgctactt caaactcaaa tttgaattag aagttcttga aattgacgaa
    10621 gttccaatgt ttaaccaaga tgaaaaatgg gatgaaagtt tccaattacg tttcttgtat
    10681 aacaagatta cacgtgctga tggtgtcatt atcgccactc ctgagcacaa ccacactatc
    10741 tcagcttcac tcaaatctgt acttgaatgg ctttcatacg aagttcatcc atttgaaaac
    10801 aagcctgtta tgattgtggg tgcatcatac tatgaccaag gaacatcacg tgctcaagtt
    10861 caccttcgta aaatccttga tgctccaggt gttaatgcct acacgcttcc aggtaacgaa
    10921 ttccttcttg gtaaagctaa ggaagctttt gataacaacg gaaatatcac caacgaaggc
    10981 actgttaaat tccttgaaac ttgcttagat aactttgtta aatacgtagg agtcgtttcg
    11041 aaattgaaaa aaccaaaacc aatcgaatca gaagacttag attgtggaaa accaatcgct
    11101 acaaccatca cagaagttga ccctgacgat ccagaatggg tagaaaaagt tgcagcaatc
    11161 actggtgctg tttctggtga tacctatgtc aaattggacc acggtatcct aacagttaac
    11221 caaattgata tgttcttgaa agctatgcca tttgaattga catacgctga cgacaacaac
    11281 caattcctct actacaacaa cgctcaccaa gatccagaca ctatgttcgc taaacgtgta
    11341 ccacctcaat caggtagccg tatgtcgact gttcatggtt ctcttccacc agcacgtatg
    11401 aagaacgtag agtgggttat cggaacactt cgcaacggaa accaagaata cgtccgtacg
    11461 atcgttccag gttctcctgc aggtgttatc aacactcaca actaccaagc aatgtactat
    11521 cctgatggat catacgctgg tatcaatgaa atcgtcttta acttccaacc atggcttgac
    11581 tggtacctaa aagagactgg tcaacgtttg gtgggtggta gcggaccatt tgctcctgct
    11641 gccggagatc atggagacgc cgatgctact tctggcgctt ctgattcagg ggatgctgga
    11701 ggccacggtg gtgacgcaga cgctacagcc ggtgcaagtt actaatgata gtttttagga
    11761 accattatgg ttcctttttt agcgaaaaaa gactaacaaa cacaaactgc tagtctcttt
    11821 gttatcgttt catctatcct tgttaaaaga aagaatactt atactcaatg aaaatcaaag
    11881 agcaaactag gaagctagcc gcaggctgta cttaagtacg gcaaggtgaa gctgacgtgg
    11941 tttgaatttg attttcgaag agtattatta tgaatttgga tcatcaagac tacggccatg
    12001 taatcctttt tcacgttgta cttggcgtag tttttctggt gtaacatcgt ttccttcttc
    12061 gtccacaatt ttgattcctt caatgtggtg acgaacagcg cggcgataac cttcgatgta
    12121 ctcctcacgt agtttggctt gttccacttt ttcttctggt gttaagcctt ctgttttttt
    12181 ctttttagca agctcattga tacgagcaat ttttttcgga tccatttctt ctcctctgta
    12241 ataagataaa gtccgtttat caaattttat ttagtgttaa tttggttaaa acaagcaaac
    12301 ttagctgctt tcttggttag ttgtgacaag attgccaaac gattttgacg gacagcctga
    12361 tcttcagcca ttaccatagt attttcaaag aaagcatcaa tgactgggct aagcgcaaag
    12421 agttgtttca attgctgact tgcaggtcct gataaaacga gtgtttctac tgcttctgcc
    12481 aaagcttttt cttggtcatt ctcaaatagt gctgaatcaa ctgtagcaac cccttctgcc
    12541 ttctcagcca ggttaaaggc acgagaaagt gattcaacag atggtttaaa atcttcttcc
    12601 ttgcttactt ctacgagagc acttgctgct tccaacatat ctgccacaac aaagtttgaa
    12661 cctgcaagaa ctgcttcctt gatatctttt ggagtagagc ccatcatctt atcaacacga
    12721 gccttgataa agtccataac ctctgcttta ttttcataag tcaaactgtc aaatttcaat
    12781 gcataaaggc tatcaatcag ctcatccata gcaatgtgcc aaccaaaggc atccaagata
    12841 cgaaccacac cttgagttgc acgacgaagg gcataagggt cattagaacc tgatggaatc
    12901 aatcctactg agaagaaact caaaatcgta tccaatttgt ctgcaatggc tagaactgca
    12961 ccgaccttgc tctctggaag ttctccttca gctgatgtag gcatgtagtg ttcacgaata
    13021 gcagctgcca ccgctggagt ttcaccagca agaagggtgt atttttcacc cataattcct
    13081 tggagttcgt caaattcacc aaccatacct gtcaacaagt caaacttgta aatggctgct
    13141 gcacgagcaa ggtcaactgt ttcatccact gacaaaccag ctttttctgc caaaagtaca
    13201 gtgatttgac ccgtacgaat catgtgttca cgaagggaac caatcttctc atggaaggtg
    13261 acattgttta atttttcaac aagatctgaa atcaccaatt tttggtcttc acgccagaag
    13321 aattctccgt cttccaagcg ggctaccaag actttttcat ttcctttgat gacatttttc
    13381 aaacgctctg cgtttccgtt acgaacagaa atgaagtttg gcaagagttt tccatcttga
    13441 tcacgaacaa caaagtaacg ctggtgttcc ttcatagaag tcaccaagac ttcttctgga
    13501 acttcaaggt atttagcatc aaaacttccc atgaaggcag ttgggtattc aaccaaattc
    13561 aagacttcat tcagcaaatc cgcatcaatt tcgatacgta caccatgttt tgcctcaatt
    13621 ttcttgattt ggtcaacaat catttgctca cgttcacatg gatctgcgat tacaaactgc
    13681 ttacgaaggt cttcttcata gctcaatgct gactgaattt tggtttcttt ccccaagaaa
    13741 cgatggccac ggctcacacg acctcccttg atatcaagga aatccaagtc aaactcttgc
    13801 tcatccaaga gaacagttaa agtgtgaaca gggcggatgt attcaaagct atttccagcc
    13861 cagtgcatgc tgacagggaa agtcaatgac ttcaagacat ctacaacacc aggaacaatg
    13921 gcttcaactg cttgaccaac ttcttcctta gtgacataga catattcttc acccttgatt
    13981 tcacggaatt cgatatcttc aacagtcaag ccttttccac ggacaaatcc ttgagctgct
    14041 ttggtgaagt ttccatcact atctaaggca attttctttg ctggacccct gaaatcttct
    14101 gtcaaatcag actgtttgtc tgcaagacca gttacacgaa cagccaaacg acgtggtgtt
    14161 gagaaggttt gaatggcttc aaaagacagg cgttttccct tgaggaaggc tgccattttt
    14221 tcgcctagtt gtttttcact tggcgtaaca acataggctg gtaattcttc aagaccgagt
    14281 tctactaata agttttttgt catgtttttt ctccgaaaag tatcttttta ttttccagtt
    14341 tgccttatgt gattggcacg caagcaacca acttcgttgt ttcattgcta aaattggagc
    14401 caaaagtctc caattttgcc tagtatcctt agtttcacta aggatacctt catcactgcg
    14461 gcaactggct cagggctcac tctgttcgcc cattttcgta atttgtcact ctctttattc
    14521 tgcgtcttct gctaggagtt tagctcttgt ttcttcatcc aaaagtgggt agcctaggcg
    14581 tttgcgttct gcgacaaagg ttttggctac gacacgggcc aagttacgga tacgagcgat
    14641 atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat taaaggtatg
    14701 tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta atgcacgacc
    14761 agcttctttt tcaaacttat caaagttttc aagcaacatt tcttggtccg aaatttcaaa
    14821 tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt ttacaccatc
    14881 agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca agcgctccaa
    14941 accataggta acttccgcag tcacagggcc agttgccaat ccaccgactt gttggaaata
    15001 agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac cagctgaacc
    15061 agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcgtgctcca aaggattgat
    15121 tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag aaggcttcat
    15181 gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat aacgaccgtc
    15241 agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc cgatagcacg
    15301 aaggaaagtg taaggactca ttgtccccgc acctttttca ttatcataag cctgcataag
    15361 catacaatct tggtcattcc aaaattgttg caaagtcaaa ataatttctt gaaatgttaa
    15421 tttcttagac attgaatact ccttaataaa aaataatttc ttgcgacacg agtctgcctc
    15481 gtcgattata cgaggcaaga cgagttagta ctgcgtctag ctcaggcact ctagtgctga
    15541 gcgtagggca gtccgactgt aaggaggttt ctgccactga cgcagtgaaa tgttaatttc
    15601 ttagacattg aatactcctt aataaaaaat aattacttgc gacacgagtc tgcctcgtcg
    15661 atcatacgag gcaagacgag ttagtactgc gtctagctca ggcactctag tgctgagcgt
    15721 ggagcagccc gactgtaagg aggtctctgc cactgatgca gtgaaatgtt aatttcttag
    15781 acattgttta ctcctttagt ttttatattt ctttttcaat taatttctgc tgaacaaacc
    15841 aatctaacaa gggaaaagtt ttttctgtat ttgcccataa atgataagaa tctaagaccg
    15901 cttgtacact acctaaattt tctaccaact tatctactgt caaaaagctg cgccagtatt
    15961 cataaaaaat acttgcatag ttcttatcat aagtcgaaga accaaaatca ttcaatgaat
    16021 gccaactata cttcttctga aaaagttcta cgagagattg attacaaatt ttttccgctt
    16081 gaaattcctc ttctgtcaag aaatacttgc gactaatata ttcaaccatc ccctcttcga
    16141 accagatata agagtcataa ccatcaaaat catctaaaaa atgtccgacc aatgagctaa
    16201 ctcatgtcct acaatctgta agaggaaatt ttcagataaa gaatgatagt gactttctat
    16261 tgcttgagtt tgctcagaac actcgtagtc catcaactga tacaaataca attctttcca
    16321 aacagctaaa tcaggcgtca taaccactcg tctattattt gtataggttg gaacagcact
    16381 ttccctaata atctgtgtag cagcattaaa atctgaccaa ataacagctt gcggtaaatc
    16441 acagactgca agttcattct ttaaaaaaga ttgataatct ttcaactttt cagtatttct
    16501 tactacaaaa tcacgaaatg tagctagttg actatcgtct tttacaagat aaaggttgtc
    16561 cacggctctc ctttctttca cacaatagac tgaataagca aaagaaccac atctgacaat
    16621 cctaggttga tttaacctag gggcgtcaga aacgcggttc caccctaatt tattacttca
    16681 ttgtttttga aaatactaca gaaagcgcca gccctttatt ttgccctact tggctcccac
    16741 tatcccaagc tcgcttgaag aacgccataa gactttcttt cctgctgact attatatcaa
    16801 aaattagaat aatgtacaat tctttttatg attttctaga aatccacatc atcaactcgt
    16861 ggagcacccg attgaacagc aatcgttttt aaggtttctc tctcctcatg actcagttca
    16921 attccaaagc aatcaagatt agcttgaata cgagaggttg tgacagattt tggaagtggt
    16981 aaaaatcctt ctgccaagct ccaggccaag gctatctgag caaccgattt tccgtgattt
    17041 gctgctattt cttggacttg cttgctatca aacagttctc cttgtccaaa aggcccccaa
    17101 gcttccaata aaattccctt ttcacgacag taagctacga cttgatcttg atacacacct
    17161 ggcgccaagc gaacttgatt gaccgcagga acgatagttg cagtttcaag caaggcatcc
    17221 aaatgatggg gaagaaaatt gctaacgccg atagcacgga ttttcccttc ttgatagagg
    17281 tcttccatcg ctctccaaac ttccgcattg cgagttttcc atgcgtcatt ttctctgagc
    17341 ggttttgggt tcggccaatg aatcaaatac aaatccaaat aatccaagcc cagtttttct
    17401 atagattttt ccaaagcttg acgagtttgc tcataggttt gctgactatt ccaaagcttg
    17461 gtagttacga acatttcttc acgtggaact ccgctatctt tgattgcttg accaacactt
    17521 tcttcattct gataaatcgc cgccgtatca atatgacgat aaccagcctt caaggcttct
    17581 aacactgcac gataggcttc ttctccatcc ttagccttaa aagttccaaa tcccaatact
    17641 ggaatttcta ctccattatt taattgatag gtattcatat gcttctcctt ctattttatt
    17701 catcaattat agcataaaac attttttaag tagtactata ttctgtaaaa aataagtctg
    17761 tcaaatatct aacagaccta ttttttcttg cttattcatc acgactatag tacagctcat
    17821 gagcttttag gcgagtattg agcatctctg gtatggtcac aaaggtataa ccttgatttt
    17881 tcaaatactc aatgaccctt ggcaaggcat tgactgtcgg actgtgaata tcatgcatca
    17941 aaacgataga gccattagct acttgatgct gaatttctgt caaaatagat gcttcatttt
    18001 tactcttcca gtccagacta tccacatccc acatgataaa gctcaaatcc aagctattgc
    18061 gaatatcatc tgtaatagca ccataaggtg gacgcatgag tttagaacta gaacccagca
    18121 ctttagttag cacatcctca gtatcagtaa tctgcttttt agcttcatca agagagagtt
    18181 gcgagagaat cggatggctc cagctatggt ttccaacaac atgaccttca gattttatcc
    18241 tcttcaccaa gtcctcattc ccagaaacat ttttcccaag cacaaagaaa gtcgctttaa
    18301 tatcatattt agctagggtc tctaatacct gcggggtcgt tgctggattt ggaccatcat
    18361 caaaggttag agcgacaact ttttgatgtt tcttatcaaa gtaagattgg tacaaggccg
    18421 catctttttc gagtaagtac gaagattgga taacatcaaa gaaagcagat actggcaagg
    18481 ctatctcttc taaattttca accacaggac ttggataaag gataatctga ctatccttgt
    18541 aatcaaaatt ccatgcagac aagtcttggt cagagaagtt ttttacaatc tgctcactct
    18601 ggtcttgctc tattttttta tcctctatga aggaggtcaa ctcttttatc agctgttcct
    18661 tagccttact agcatctgaa aacagttggt caagtgtaaa aggttgccca tcttctgtta
    18721 agtgaacctt tcctagactg gtcttttcag tctcttcaat cttaaaagca actaaatcat
    18781 agatttgttt ggtcacatta cgattaacga ctccctttaa acctgactct tcttgctctg
    18841 tatagtagaa aacaagattg tccttacttt ctagcttgtc cttaacatct tgatttatca
    18901 gctccctaac agaggaaatc actttctccc cttggagagg ataataggca atcacttcgg
    18961 cttgcccctg acgaaaatgc tccttctgat ttccctcact caattgatca tctttctctt
    19021 ttttgagcga ttcaatcttt tgctcaaaac ttttttgctg gtagatctta aaagcaataa
    19081 atccgcctaa taggcaaata gaaattgcta aaataccaat caaagccaat aatacatgtc
    19141 tcgttttccc gtgtctgcca cgtcctagtc tacttttatt catatttata tcataacaaa
    19201 tttgagcaat aatttcaatg atacaatttg gcaatccaca attcctctag caagccaaag
    19261 catcttacat caactccaac atccgtatct gtagagatta tgtagttttc tatctatgga
    19321 atttctagta gattgaagct agaatagtag acctatactt ctaaaacatt gttagaaatc
    19381 gatttgactg tcctgatcga tttgtcctgt tctcatttca ttttactata aaactatgaa
    19441 aagctaggtc atctgagaag taattactat cgacaattgc gatagcgtat actagtgcga
    19501 tttgtttgta gctctggtgg gtatcacctt cacgttggcg aattttttct aggttttgat
    19561 taacctgact gcacagataa ggccataggc gattgatgaa aaaattctga tgatgaacct
    19621 gaatttcgat gataacttgg attccattat ctaactccgc caagacgtct atactagtat
    19681 agaaatctta cgctgagtag ggcatggaag gcaagacgtg aatgttactt ccctccaaaa
    19741 tcgtcacatt ttttgctggc aagtccagca tatcgcggat aaattgacaa atcattagtt
    19801 gggctgatac ctagatgtct gagaatcctc cttttcctcc ttctattata gcacattttt
    19861 aaatctaggt ttgctagatt ctatgcttct atctatttat tcggaaaaga aagctaatag
    19921 cttatttgaa attttctcct ctctgttgga tagtcccgtc tatctatgtt ataatgaaag
    19981 aaggatttaa aatcggaaaa ggagtattta tgctaaaatt aggtgtcatc ggaactggcg
    20041 ctatcagcca tcatttcata gaagcagccc ataccagtgg agaataccaa ctggtcgcaa
    20101 tctattctag aaaactagaa actgcagcaa cctttgcttc tcgctatcag aatatccaac
    20161 tattcgatca attagaagtc ttcttcaaat cttcttttga tttagtctat atcgccagtc
    20221 caaactcctt acattttgct caggcaaaag ctgccttgtc tgctggtaaa catgttattc
    20281 tcgaaaagcc agctgtctct caaccacaag aatggtttga tttgattcaa acagctgaaa
    20341 aaaataactg ttttatcttt gaagcagctc gtaattacca cgagaaagcc tttactacta
    20401 tcaaaaactt tttagcagat aagcaagtgt taggagcaga tttcaactat gccaaatact
    20461 cttccaagat gcctgacttg ttggctggac aaacaccaaa tgtcttttca gaccgttttg
    20521 ctggtggagc cctcatggat ttggggattt atcccctcta tgctgctgtt cgtctttttg
    20581 gaaaagccaa tgacgcgact taccatgctc aacagcttga caatagcatt gacctaaatg
    20641 gagatggtat cctcttctac ccagactatc aagttcacat caaggctgga aaaaacatca
    20701 cttccaatct tccttgcgag atttatacaa cagatggaac cttgactctc aacacgattg
    20761 agcatattcg ctcagctatt tttaccgacc accaaggaaa tcaagtccag cttcctatcc
    20821 aacaggctcc tcatacgatg actgaggaag tcgctgcatt tgcacacatg atccagcaac
    20881 cagatctgaa tctctaccaa acttggctgt atgatgcagg ctctgttcat gagctactat
    20941 ataccatgcg ccagactgct ggcattagat ttgaggcaga aaaatgaaaa ccaaactacc
    21001 gactgaatgg caagaactga gtgaccaact cggtttccaa gaattcaccc ccattcaaac
    21061 tcaactattt gagccccttc ttgctggaga aaacctccta ggagtgagcc aaaccggaac
    21121 tggtaagacc ctagcgtatc tcctaccaag tcttctcaga ctacaaaaga aaaaagccca
    21181 acaactcttg attctagcac caaatacgga acttgctgga cagatttttg atgtatgtaa
    21241 aacgtgggca gaagctatcg gattgactgc tcaactcttc ctttcaggtt caagtcagaa
    21301 acgacaaatt gaacgtctta aaaaggggcc agaaattctg attggaactc ctggccgtat
    21361 ctttgaactc attaaactta aaaaaatcaa gatgatgaat gtggaaacca tcatcctgga
    21421 tgaatttgac caattactag atgattctca gattcacttt gtagaaaaaa tcactcacta
    21481 cgcacctcgt gaccaccaac tggtctacat gagtgccacg accaagtttg accaagaaaa
    21541 gattgtgccg aatacacgca ctattgatct ctctgatcaa aaattggaca atatccagca
    21601 tttctacatg caggtagacc aacgtcatcg agtggatatg ctacgaaaac tggctcatgt
    21661 agaggatttc cgtggtctag tcttcttcaa tagcctgtca gaccttggga atgccgagga
    21721 aaaactacag tatcgtgata tattggctgt ttccctcgct agtgatgtta atgttaaatt
    21781 tagaaaaatc atcttagaaa agtttaaaga taaccaacta accctgcttc ttgcaactga
    21841 ccttttggct cgtggaattg atatcgatag cctagaatgt gtcgtaaact ttgatattcc
    21901 tagagatagc gaaacctaca cacaccgtgc tggccgtact ggccgtatgg gcaaggaagg
    21961 atatgttatt actctcgtca ctcatccaga agaaatcaaa aaacttaaaa agtttgcaag
    22021 tatccgagaa attgtcctaa aaaaccaaga actctatatc aaataaagcc ccattgctct
    22081 atacacatcc agtgtatgga gcttttttat aagcaattca attttatact catctgcttt
    22141 caaaaagcat tctagtccat ctctgattaa cgatggactt tatcacctcc ttctccagtc
    22201 cttgtatgac atcttgaagt tgattcatga catcttccaa agttcgaaag gctttattct
    22261 taaatccacg tttacgaatc tctttccaca cttgttcaat ggggttcatc tctggtgtgt
    22321 atggaggaat aaatgcaaag ccaatattag tcggaatctt taaggtactt gatttatgcc
    22381 atatagcatt gtccataacg agtaaaaaat aatcatctgg ataagcttgt gaaagctctt
    22441 ctaaaaagac gttcatccac tcagtattac atccaccagc tcttaagaaa aatgattcgc
    22501 ctgtatgggc atcaacagct ccataacaat agcgaaattc tcgtatatag tgactatgga
    22561 catgtggacc tactcctatt ggagaccaac aagatcccag tttactgatt ctaccgaaac
    22621 cagcatcatc ttggtacatc aagcgaacct tatgaaaagg tctactggtt ttaaagcgct
    22681 ttcttgtgta ttgcccccca aaagttagac aattaattta tccgaaggat ttagttctgt
    22741 attgcacagg gctaagtcct tttagtttta tcttaatttt cttattgttg ta
//
LOCUS       DAVMHG010000018        22733 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMHG010000018 DAVMHG010000000
VERSION     DAVMHG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.018"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            89..742
                     /locus_tag="VYR08_000571"
     CDS             89..742
                     /locus_tag="VYR08_000571"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV3466143.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            752..1216
                     /locus_tag="VYR08_000572"
     CDS             752..1216
                     /locus_tag="VYR08_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3466144.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1229..2497
                     /locus_tag="VYR08_000573"
     CDS             1229..2497
                     /locus_tag="VYR08_000573"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV3466145.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2511..2771
                     /locus_tag="VYR08_000574"
     CDS             2511..2771
                     /locus_tag="VYR08_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466146.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2782..4143
                     /gene="pdrM"
                     /locus_tag="VYR08_000575"
     CDS             2782..4143
                     /gene="pdrM"
                     /locus_tag="VYR08_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV3466147.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4297..5265
                     /locus_tag="VYR08_000576"
     CDS             4297..5265
                     /locus_tag="VYR08_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV3466148.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5281..6273
                     /locus_tag="VYR08_000577"
     CDS             5281..6273
                     /locus_tag="VYR08_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV3466149.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6491..7534
                     /locus_tag="VYR08_000578"
     CDS             6491..7534
                     /locus_tag="VYR08_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV3466150.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLATSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7598..9283
                     /gene="lpdA"
                     /locus_tag="VYR08_000579"
     CDS             7598..9283
                     /gene="lpdA"
                     /locus_tag="VYR08_000579"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV3466151.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9347..10336
                     /locus_tag="VYR08_000580"
     CDS             9347..10336
                     /locus_tag="VYR08_000580"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV3466152.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10409..11479)
                     /gene="xerS"
                     /locus_tag="VYR08_000581"
     CDS             complement(10409..11479)
                     /gene="xerS"
                     /locus_tag="VYR08_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV3466153.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(11827..13389)
                     /locus_tag="VYR08_000582"
     CDS             complement(11827..13389)
                     /locus_tag="VYR08_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV3466154.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISK
                     APLTAMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEG
                     EVTLIEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIP
                     KSEIGKVKDLLL"
     gene            complement(13405..14184)
                     /locus_tag="VYR08_000583"
     CDS             complement(13405..14184)
                     /locus_tag="VYR08_000583"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV3466155.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(14171..15022)
                     /gene="ylqF"
                     /locus_tag="VYR08_000584"
     CDS             complement(14171..15022)
                     /gene="ylqF"
                     /locus_tag="VYR08_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV3466156.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15241..15417)
                     /locus_tag="VYR08_000585"
     CDS             complement(15241..15417)
                     /locus_tag="VYR08_000585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3466157.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(15469..21255)
                     /locus_tag="VYR08_000586"
     CDS             complement(15469..21255)
                     /locus_tag="VYR08_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3466158.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYHEETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPKYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGNSTEDVSTESNTSNSNGNE
                     EIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFVK
                     VKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEIESRYKDNFTFYLAKKGTE
                     ETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDG
                     KHYAIYNLKKPLFESLRGATIEKLSLKNVSISGKDDIGSLANEAQNNTKIKQVHVDGV
                     LAGERGIGGLLAKADQSSITESSFKGRIVNTYETTAAYNIGGMVGHLTGDKALLTKSK
                     ATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRT
                     SGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFSSKDNRVYNVTLVKDEVVS
                     KESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKKDSDFSKVAYYQAKRNLTY
                     KNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVVMKDNQVITDIVSNKQTANKL
                     LLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQFLYDQTSIIKQVLLDLQKV
                     DYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLKKLLSADAGLASANPVTEG
                     YLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYHLDFFGKGNASPLDTLIEL
                     GKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAY
                     IVEEKSTIEEVKTKQGLASTKYSIGVYDRITSATWKYRNMVLPLLTLPERSVFVISTM
                     SSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDHYDYWYRILDDNAREKLYR
                     NILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVGNKVGHNQHGAYATGDAVY
                     YMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDHPDDAT
                     ITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVHNMFDVVYMLEYLEGNSIL
                     KLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEEVERLRSFNDLIDNNILSS
                     REYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRRIAYELLAAKGFKDGMVPY
                     ISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQYHTWSEFKKAMYQERQDQF
                     DRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKDAEHNYYHWNNYNPDIDSE
                     VHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(21464..22642)
                     /locus_tag="VYR08_000587"
     CDS             complement(21464..22642)
                     /locus_tag="VYR08_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466159.1"
                     /translation="MTLPVRKSLHDAVLQASKANTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         6462 a         4445 c         4676 g         7150 t
ORIGIN      
        1 tttattaaat ctttagataa attgtaaaaa gtggtttcca tagccacttt ttgctataat
       61 agaggcagta aaaatgaagg agtcagctat ggaacactcg tcttggcatg ctttgattaa
      121 ggcgcaatta cctgagggtt atttcgggaa aatcaatcag tttatggagc aggtctattc
      181 tcaggggatt atttatccac ccaaggaaaa ggtttttcag gctctcttga caacactgct
      241 tgaagaagtt aaggtggtaa ttctagggca agacccctat cacggaccag gtcaagcgca
      301 gggcttgagt ttttctgtac ctgactctat cccagctcca ccatccttgc aaaatatctt
      361 gaaagaattg tcagatgata tcggagttaa gaaatctcat gatttgacag cttgggctga
      421 gcaaggagtc ttgcttctta atgcttgttt gactgttcct gctggacagg ccaatggtca
      481 tgctggtcaa atctgggagc cttttactga tgctgtgatt caggtggtca atcatctaga
      541 tagaccagtc gtttttgtac tctggggagc ttatgcacgt aagaagaagg ccttagttac
      601 caatcctcat cacttgatta tcgaatcagc ccatccaagt cctttgtcgg tttatagagg
      661 attttggggt tccaagccct tttccaaggc caatgcattc ttaaaagaga caggacaaga
      721 gccaatcgat tggcttagat aaggagaaaa tatgcctcag ttagcgacga tttgctacat
      781 tgataatggg aaagaactgc tcatgctcca tcgtaataag aaacccaatg atgtccatga
      841 agggaaatgg attggtgtgg gtggtaagct agagagagga gagacgcccc aggaatgcgc
      901 ggcgcgtgaa atccttgaag aaacagggct caaagccaag ccagttctaa aaggtgtcat
      961 cacttttcct gaatttacac cagatttaga ctggtacacc tatgttttta aggtgacgga
     1021 gtttgagggc gacttgattg actgcaatga ggggacgcta gaatgggttc cctatgatga
     1081 ggttttgagc aagccgactt gggaaggtga ccacaccttt gttgagtggc ttttagagga
     1141 taaacccttc ttttcagcca agtttgttta tgatggggat aaattgttgg atacccaagt
     1201 tgatttctat gaataaagga gaaagcagat gctactaatc aaaaatggtc gtgtaatgga
     1261 tcccaagtct ggtttggatc aagtttgtga tgtcttagtt caagatggga aaattatcaa
     1321 aattgcgtct gagatcacgg aagaaggagc agaaacgatt gatgctactg gtcttgtggt
     1381 tgctcctggc ttggtcgata ttcatgttca tttccgtgaa cctggtcaaa cacataaaga
     1441 agacattcat actggtgccc tagcagccgc tgcaggtggt tttactactg tcgtcatgat
     1501 ggctaatacc agtccaacca tttcagacgt ggagactttg caagcagttc tccagtcagc
     1561 tgccaaagag aagattaatg tcaagacagt tgcgaccatt actaaaaact ttaatggtaa
     1621 aaacttgact gactttaagg cactcttaga agctggtgcg gttggtttct ctgatgacgg
     1681 tattccgctt gagagcagta agattgtcaa ggaagccatg gaggaagcca aaaaactcaa
     1741 tacctttatc agccttcatg aggaagatcc aggtttgaac ggtgttcttg gctttaatga
     1801 aaatattgct agagaacatt tccatatctg cggtgctact ggggtggctg agtacgctat
     1861 gatggcgcgt gatgtcatga ttgcctatgc aactaaagcc catgttcaca tccagcattt
     1921 gtctaaggaa gaaagtgtta aagtagtgga gtttgctcag gggttaggtg cagaagtcac
     1981 agcagaagta gcgccacagc atttctctaa gacagaagca cttcttttaa cacaaggtag
     2041 caatgctaag atgaatccac cgcttcgttt ggaatcagac cgtcgtgccg ttatcgaagg
     2101 tctcaaatca ggtgtcatca cagttattgc gactgaccac gcgcctcatc atgtagatga
     2161 aaaaaatgtt gaggatatta ccaaagcgcc atctggtatg actggcttag aaacatccct
     2221 gtctctcagc ttgacctatt tagtagaagc tggtgagttg agcttgatgg aattacttga
     2281 aaaaatgaca tacaacccag ccaagcttta caactttgaa gcaggttact tggctgagaa
     2341 tggtccagca gatatcacta tttttgatgc caaggctgac cgctttgtgg actcccattt
     2401 tgcttccaaa gcagctaatt caccattcat cggtgaaacc ttaaaagggc aggttaaata
     2461 taccatctgt aagggacaaa tcgtctatca agcttgatag gagttagaat atgaattatt
     2521 ttcgaaaacg tagggagaga caagcgaaat ctaatagtgg aatttactgt cccgcagcta
     2581 atcgttccaa aattcaatat gaaactaaag aaaaagctga ccatgcggtt caatatgata
     2641 acggaggttt agttagaagc tattattgtc gtacatgtgc gtgttggcat actacttcaa
     2701 aatctaataa acctccgcta agtttgaaaa actatggtct gaaactcttt ggtttagata
     2761 aatcagaata gaaagagaag tatgtataag acaaagtgtt tacgagagaa gttagtatta
     2821 tttttaaaaa ttttcttccc aatcctgatc taccaatttg ccaattattc tgcctctttt
     2881 gttgatactg caatgacagg tcaatacaac actatggact tggctggtgt atctatggca
     2941 accagtatct ggaatccttt ctttacattt ctaacaggga ttgtgtcagc cttggtgcct
     3001 atcattggtc accatcttgg tcgaggcaaa aaggaagaag ttgcgtctga tttttaccaa
     3061 tttatttatt tggccttggg cctatctgtg gtcttgctgg ggatggtact tttcttggca
     3121 ccaataatct tgaatcatat tgggttagaa gcagcagtag cggcagtagc ggttcgctat
     3181 ctttggtttt tatctatcgg gattatcccc ttgttgctct ttagcgtcat tcgttccttg
     3241 ctggattcgc tgggcttgac caaactgtcc atgtacctca tgcttttgtt actccctctc
     3301 aatagcggat ttaactatct cttgatttac ggtgcctttg gtgttccaga actgggaggg
     3361 gctggtgctg gtttaggaac atccttggcc tactgggtct tgcttgggat ttctgttctg
     3421 gttttattta aacaggagaa gctcaaagcc ttacaccttg agaaacgaat tccacttaat
     3481 atggataaaa ttaaggaagg agttcgttta ggtctgccta ttgggggaac tgtcttcgcg
     3541 gaagtggcta tcttttcagt ggttggcttg attatggcta agttttcgcc cttgattata
     3601 gctagtcacc agtcagctat gaacttttca agtcttatgt acgcctttcc tatgagtatc
     3661 tcatcggcta tggctattgt cgtttcctat gaagtgggag ccaagcgatt tgatgatgcg
     3721 aaaacctata ttggtctagg aagatggact gccctcattt ttgcggcctt caccttaacc
     3781 ttcctttaca tttttagggg aaatgtggcc agtctttatg gtaacgaccc aaaatttatc
     3841 gatttgacag tgcgtttttt aacttatagt cttttcttcc agttagcaga tacctttgcg
     3901 gcgccgcttc agggaatttt gcgggggtat aaggatacag ttattccttt ttaccttggt
     3961 ttgcttggtt attggggcgt agcaatccct gtggctacgc tatttgattc cctaacagat
     4021 tttggagctt attcttactg gatcggcttg attattagtt tgattgtgag cggggcgctc
     4081 taccgttggc gtttaactgt gattatgaag agatttgaat ctttagcaaa atccaaatgc
     4141 taaaaagttt gtgaaaaaat attcttgaca agaaaatccc ttgttttcac ggggttttct
     4201 tttttatttt gtgaaatttc ttttagaaaa actttgacag tatttgccaa aagcggtaaa
     4261 atagtaaggt aagaagaaag ttagaaaggc aagaagatgt caactttaga taaaaatctt
     4321 ttgctagaaa tgttccgtaa gatggaagaa atccgtcgca tggacttaaa aattgcacaa
     4381 ttagtaaaga aagggaaagt gccaggaatg acgcactttt ctgttggtga agaggcagct
     4441 aacgtggggg ctatgttagc tctcaatcca gatgatctga ttacctcaaa ccaccgtgga
     4501 cacgggcaag ctattgctaa agggattgac ctcaacggaa tgatggctga aatccttggt
     4561 aaatacactg gaacctgtaa agggaaaggt ggatctatgc atatcgctga ccttgatgct
     4621 gggaaccttg gtgccaatgg tatcgtaggt ggtggtatgg ggatcgctgt cggtgcagcc
     4681 ctcagtcagc aaatgcaaaa taccggtaaa atcgttgtct gcttctttgg agatggtgcg
     4741 accaatgaag gtgttttcca cgaagcagtg aacatggctt ctatctggaa cctgccagtc
     4801 attttctatt gcattaacaa cggttacggt atctctgcgg atatcaagaa aatgaccaat
     4861 atagaacata tccatcaacg tagcgccgct tatggaattc ctggaatgtt catcgaagac
     4921 ggtaacaatg tcatcgatgt ctatgaagga tttcagaaag ctgtggacca tgttcgcagt
     4981 ggcaatggtc cagtcttgat tgaaagtgta acttatcgct ggctcggtca ctcatcatct
     5041 gaccctggta aatatcgtac gcgtgaagaa gtggaattgt ggaaacaaaa agatccaatc
     5101 gaaaacctcc gcaattacct tattgaaaat aacattgcaa gtgccgaaga attggaagaa
     5161 atccaagcgc aagtaaagga agcagtagaa gcttctgtta aatttgcaga ggaaagccca
     5221 ttcccaccgc ttgaatcagc ctttgaagat atttacgcag actaaggaga aagagataaa
     5281 atggaaacaa aaacaatgtc cttccgtgac accattatcc ttgctatgtc tgaggaaatg
     5341 cgtcgcgatg aaaatgtgtt cttgatggga gaagacgtcg gtgtcttcgg aggagacttc
     5401 ggtacttctg ttggaatgct tgaagaattt ggtccagaac gtgtccgtga ctgtccgatt
     5461 tctgaagctg ccatctctgg agcagcagca ggagcagcca tgacaggact tcgtccaatc
     5521 gtcgatatga ccttcatgga cttctctgtt attgccatgg acaatatcgt caaccaagct
     5581 gctaaaacac gttacatgtt tggtggtaaa ggtcaggttc caatgactgt tcgttgtgca
     5641 gctggtaacg gagttggctc tgccgcccag cactcacaat ctctagagtc ttggtttact
     5701 cacattccag gacttaaggt tgtggcacct ggaacacctg cggacatgaa aggcctgctt
     5761 aagtcttcta tccgtgataa caacccagtt attatacttg agtacaagtc agaatttaac
     5821 caaaaagggg aagtgccagt tgatccagac tacacaattc cacttggggt tggtgaaatc
     5881 aaacgccaag gaacggatgt aacagttgtt acttatggaa aaatgcttcg ccgtgtggtt
     5941 caagctgctg aagaattagc agaagaagga atttcagttg aaattgttga cccacgtacc
     6001 cttgttccgc ttgataagga tatcatcata aactcagtga agaagactgg taaggttgtt
     6061 ctggtcaacg atgcccacaa aacaagtggc tatatcggtg aaatttcagc tattatttca
     6121 gaatcagaag catttgacta tctagatgca ccaattcgcc gttgtgcagg agaagatgtg
     6181 ccgatgcctt atgcacaaaa cctagaaaat gcaatgattc caacagttga aagtatcaaa
     6241 gatgcaatcc gaaaaactta taacaaagaa tagggttaca taagataagt tatgtaaata
     6301 aaataaagct gacgagaaac tttgtatctt ttaggtgcag agctcttcgt cattaatatc
     6361 ttagattaga gcacgggcta aaagatcgga aaaaagataa atctccttgg cttcatcgaa
     6421 gccatcgtcg atttcctatt tttcagtcgc ttttgacgcc ctttgtatca taaatagaat
     6481 tggagaggtc atggctgatg acaagctaag agcgactcct gcagctagaa agttagcgga
     6541 tgatttaggg ataaacctct acgacgtttc tggctcaggt gcaaacggtc gtgtccacaa
     6601 agaagacgtg gaaacttata aagacacaaa cgtggttcgc atttcgccac ttgcaaaacg
     6661 aattgccctc gaacataaca ttgcttggca ggaaatccaa ggaaccggtc atcgtggtaa
     6721 aatcatgaag aaggatgttt tggccctgct tcctgaaaat atcgaaaacg atagcatcaa
     6781 atctcctgct cagattgaaa aagtggaaga agtccctgat aacgtaacac catatggtaa
     6841 aatcgagcgt attccaatga caccaatgcg taaggttatt gcccaacgca tggttgaatc
     6901 ttacttgact gcgccaacct tcaccctcaa ctatgaagtt gatatgactg aaatgttggc
     6961 tcttcgtaag aaggttcttg agccaatcat ggaagcaact gggaagaaga ctactgtaac
     7021 agaccttctt tcgcttgcag ttgttaagac tcttatgaaa cacccataca tcaacgcttc
     7081 attgacagaa gatggcaaga ctattatcac tcacaactat gtcaatcttg ccatggcagt
     7141 tgggatggat aatggattga tgacacctgt tgtttacaat gctgagaaga tgagtctttc
     7201 agaactggtt gtagccttta aggatgttat tggccgtacc ttggatggta aattggctac
     7261 aagtgagtta caaaattcaa cattcacaat cagtaatttg ggaatgtttg gtgttcagtc
     7321 ctttggtccg attattaacc aacccaactc agctatcctt ggtgtcagtt cgacaatcga
     7381 gaagccagtt gtcgtcaatg gtgaaattgt gattcgccca atcatgagtt taggattaac
     7441 cattgaccac cgtgtcgtag atggtatggc tggtgctaag tttatgaagg acttgaaaga
     7501 gttgattgaa acgccaatct caatgttaat ttaagagcaa gagtattcat ttttaagata
     7561 tagataaagg aaggaagaca tggccttaga agtaattatg ccaaaagccg gcgtggatat
     7621 gacagaagga caaatcgtcc aatggaataa aaaagtcgga gaatttgtaa aagaaggaga
     7681 aatccttttg gaaatcatga ctgataaagt cagcatggaa ttggaagccg aagaagatgg
     7741 gtacttgatt gccattctca aaggagatgg tgaaactgtc cctgtaacgg aagttatcgg
     7801 ttaccttggt gaagaaaggg aaaacatccc aacagctgga gcagcatcgc cagaagctag
     7861 ccctgtacct gtagcaagta cctcaaacga tgatggtaag agcgatgatg cttttgatat
     7921 cgttgtgatt ggtggaggtc ctgctggtta tgttgcagcc attaaagctg cccaattcgg
     7981 cggtaaggtt gcccttgttg agaaatctga acttggtgga acctgcttaa accgtggatg
     8041 tattccaacc aagacctacc ttcataacgc tgaaattatt gaaaatatcg gtcatgctgc
     8101 aaatcgtggt atcgtgattg aaaatcctaa cttcactgtt gatatggaaa aacttttaga
     8161 aactaaatct aaagttgtta atactcttgt tggtggtgtt gcaggacttc ttcgtagcta
     8221 cggagttact gttcataaag gaattggtac aatcactaaa gacaagaacg tcttggtaaa
     8281 tggttctgaa ttgcttgaaa ccaagaaaat cattcttgct ggtggttcaa aagtcagcaa
     8341 gatcaacgtc cctggtatgg aatctccact tgtcatgact agtgatgaca ttcttgaaat
     8401 gaacgaagtg ccagaaagcc ttgttatcat cggtggtgga gttgtcggta tcgaactcgg
     8461 tcaggccttc atgacatttg gttcaaaagt gactgttatc gaaatgatgg accgtatagt
     8521 tccagctatg gatgtggaag tttctaagaa ccttcgcttg attcttgagc gtaaaggaat
     8581 gaccatcttg actggtacta aactgcaaga aatcattgag gaaaatggtc aacttcgtat
     8641 caaggttgaa ggaaaagacg atatcatcgc aagcaaagct cttctttcaa ttggtcgtat
     8701 gccagacctt gaaggtattg gagaggttga gtttgaattg gatcgtggtt gtatcaaggt
     8761 caacgaatac atggaaactt cagttccagg catttatgca ccaggtgaca tcaacggtac
     8821 taagatgttg gctcacgcag ctttccgcat gggtgaagtt tccgctgaaa atgcccttaa
     8881 aggaaatcat gcagttgcca aattgaattt gactcctgca gccatctaca ctctccctga
     8941 agtagcagca gtaggtttga cagaagaaca agcccgtgag aaatacgatg ttgccatcgg
     9001 taagtttaac tttgctgcta acggtcgtgc tattgcatct gacgcagctc aaggtttcgt
     9061 aaaagttatc gctgataaga aatacggaga aatccttggt gtacacatta ttggtcctgc
     9121 agccgcagaa ttaataaacg aagcatcaag catcatcgaa atggaaatca ctgttgagga
     9181 aatgctgaag accatccacg ggcacccaac ttactctgaa gtgatgtacg aagcgtttgc
     9241 agatgttcta ggaatggcca tccattcacc taagaaaaaa taaaacgaag atagattaga
     9301 ctggaaggtt atttccagtt tctatcatat aaagtaaagg gatatcatga aatacattat
     9361 caatcattca aatgacactg cttttaatat tgccttggaa gaatacgcct ttaaacacct
     9421 tttggatgag gatcaaatct tcctactttg gattaacaag ccatctatca ttgttggtcg
     9481 tcaccagaac actatcgaag aaatcaaccg tgattatgtt cgcgaaaatg gtattgaggt
     9541 agttcgccgt attagtggtg gtggagctgt ttaccacgat ttaaataacc tcaactacac
     9601 gatcatctcc aaagaagatg aaaataaggc atttgacttc aagagcttct caactccggt
     9661 tatcaatacc ctggctcaac ttggggttaa agctgagttc acaggtcgta atgaccttga
     9721 gattgatggt aagaagttct gtggcaatgc ccaagcctat atcaacggcc gtatcatgca
     9781 ccacggttgc ttgctctttg acgttgattt gtcagtcctc gcaaatgccc ttaaggtttc
     9841 aaaagataaa tttgaatcaa aaggtgtgaa atccgtccgt gcccgtgtaa ccaatattat
     9901 caatgaatta ccaaaaaaaa tcacagtcga aaaattccgt gatttactct tggaatacat
     9961 gaaaaaagag tacccagaga tgactgaata cgtcttttca gaagaagaat tggccgaaat
    10021 caatcgcatc aaggatacta agtttggaac ttgggactgg aactatggta aatcacctga
    10081 atttaacgtc cgtcgtggaa tcaaattcac tagtggtaag gttgaagtct ttgctaacgt
    10141 tactgaatca aaaatccaag acatcaagat ttatggtgac ttctttggta ttgaagacgt
    10201 tgctgcagta gaagatgtcc ttcgtggggt gaaatacgaa cgcgaagatg ttcttaaggc
    10261 gctaaaaacc attgatatca cacgctactt cgctggtatt agccgtgaag aaatcgctga
    10321 agcggtagtt ggataaatca aaaagaagtt ggccaactaa ccaacttctt tttttgatat
    10381 ttatttacat aatttaaaat acgtaaaatc ataaactatc cagagcattc ttttgttcat
    10441 cactaacaat atgggtatag aggtcagtga cttgtgtgct agcatgtcct agttggtgac
    10501 tgactaaaac ttgtgattta gtcgcatcat agagcctagt tgctagtgta tggcgcagtt
    10561 tatggggtgt tacacgcact ttaaaatcct ctgagtattt agcaaccatt ttctcaacgc
    10621 tagaagcatc gatacgatta ggaacacctc tgtagagagt taaaaaaagg gctgtatctg
    10681 ttttttccgt tttatagcgt tgattccgaa tggccagata attctctaaa taaggtttag
    10741 caaaagcagc gacattgact gagtcacgtt tgcaaccttt tcgagtaaca tcaataacca
    10801 tcatttttag attgagatct cttagatcta gattaacagc ttcagataag cgaacaccag
    10861 atgccaagag aagggcaata atggctaaat ctcgttcttt atttttgttg aatgatgaga
    10921 gagctcgatt tgaaagctgt tgtgggtgct cttgatcgat ataagttaga aaaccttctg
    10981 tttcatcacc tagaaagagt ttttgcttga tattttcagc tctggcagca agggtttctt
    11041 tctttttctt ggtggaaact tttttcatta cattacgata gaaataaggt tccccctgat
    11101 cgttttcaac ctcctcggtt agatacttgt aaagactaga aagtgctgat aaggttcgat
    11161 tgatagttgt ctgtgaaaca ccttgttttg ttgtattagc attcagcaag ggacgttcac
    11221 gtagataaag gataaaggat tccatgtctt tcttagacat attttccaaa actgataaag
    11281 gaatatcgga tattttatca gcgtttgaaa taccagactc caaaacccag ctgaaaaatc
    11341 ggtcatattc cttaaggtat tcgtacaggg ttgtaaaact gtagggcaca gccagcttag
    11401 attggtagta ttccagaaca taccagggca tgagttgttt tagtttgtcg attcgttcca
    11461 gtaaaatctc acgtttcatg tattttctcc ataagtaagt atactagtag tatagcatag
    11521 acttgtatat ttttcaagaa aattatagtt tttcggaaag atgttatagg agtttttcaa
    11581 agtgataaaa agacttagac ctacaaacaa aacagaataa ggaaaataat aaaaatctca
    11641 cccactaaac ttctgttaat actacataac tgaagagcta tataaggagc tatgagatac
    11701 aaaacacatt gtataataga ttgaaactag aatagtacat ctggacttct aaaacattgc
    11761 tagaatcggt tttctaaata aatcaaactg atcatttaca taacataaat tatgtaaata
    11821 ctcatactac aacaacaaat ctttgacttt tccaatttca ctttttggaa taaccaggtg
    11881 aatcatatca cccagataca ttctggttga gccgttaact gtttggctct tgccattatg
    11941 gacttgagtt gtgatgagga cgttgtgtgg taagttgagt tcatgaactt gtttcccagc
    12001 aattttatca gaaactggta tttcgataag tgtaacttct ccttcgctag atacttcttc
    12061 tggaagcatt ttttccagca tggcttcata gactggcgta cctttgagca aatccatgat
    12121 aatataagaa acaagagtga caagaccaag tggcataagg ttgcgaatat ctcctaccat
    12181 ctcagttacg aggatcatag cggttaaggg agcttttgat atggctccaa aatagccact
    12241 cattcctaga atgacaaata tagggaattg ctcttgactg acaagtccaa gattgacaca
    12301 gataacacca actaaggcac caagcaaaga accaagcaaa gaaccaagag ctaaaatggg
    12361 gaggaaaatt cctcctggca gtccacttcc atagctaatc atactccaaa taaagcggat
    12421 taaaaagtaa gctaataaaa cttggaaact aaaattttgt tcagttaaag aaaggacaag
    12481 ctgatttccg ccaccaatga tttgaggtaa gaagattccg actggtatga taaggataaa
    12541 agccaagatg ggataataag ccctatccaa atggattttt tgaccaatca agtcataaac
    12601 tcttccaacg tttaatacag ctttctcata gagaaaacct gaaaatccaa ggaaaattcc
    12661 catgacgaga tatatccaat actgatctag ggtcatggga ggaatgttat ctggcatatc
    12721 caatactggt gtcaaaccga acatgagtag agacacaaag tttgctacga tgctggctgc
    12781 tagagttgag acccagaaaa agcgcgaaaa atggtgatag acttcttcta caacaaagag
    12841 aagtgctgca ataggagcat taaaggctgc ggctaaacct gctgcagctc cactggcaat
    12901 caaggaacgt tcctctactg gactggattt gagccacttg gcaattcctt taccaccaac
    12961 tgctccaagt tgaatgctgg gtccctctcg acccagcatg agtccactgg caatagcaag
    13021 aatacctagc acatattttt tccaaagaat gccccaccag ttgagggaca tgaggccttt
    13081 cagttcggct tcgacttgag gaattcctga gcctttaata tctttttctg accgtgttag
    13141 tttggcactg agccaacaga tgagtatata aaacaaaacc agtacaaaaa gattgcgcac
    13201 taagtaccct tgatcttgat aaactccttg tatcaggtgg aagccctttt caattaagaa
    13261 acgaaaggat ccgacgataa ttccaacgat gaggccgaca atgattcctc gaccaacttg
    13321 ggatagtata gtgctggatg caaaggcgaa ttctttctta gaacggacta tttccgactg
    13381 ttcctccatg ttatttcctt tcaattaact ttctttttta cctaaaacca gtgatttaac
    13441 gggttcaaag ctggttcggt gaattggggt aactcctagt tttgtgaggc cttccagatg
    13501 tttagctgtg ccatatcctg cattagtagc gaaatcatag ccagggaact gctgatcgta
    13561 ttctttcagc aattcatcac gtgttacctt ggctactata gatgctgctg cgatagagag
    13621 ggagttggca tctcctttga taatggaggt ttgtgaaatg ggcaagtcca gtttcatggc
    13681 atcaatcaaa aggtgctctg gttgagggct gagctgggag attgcttctt gcatggctag
    13741 tttggttgct tcatagatgt tgacttggtc gatgacctga ttatctatga taccaattcc
    13801 aatcgacaag gcttggtctt gaacggcttg gaaaatctcc agatgtttct ttttaggaat
    13861 tttcttgctg tcgttgagac ctttaatctt acaattttta gataaaataa cggccgcagc
    13921 gactacagga ccagcaagag gaccacgacc aacctcatca atacctgcaa ttaaggtcaa
    13981 tccttgctta taaagttctt tttcataaga aagcatggat tccaagcgca aattttcatc
    14041 taattcagct tgaatggctc ttttacgctt gctgatttcc ttttgaactc ctgagcgatt
    14101 atccttttca agctctaaaa aaatagggct ttctaactcc ttgactgtca caaggaattc
    14161 tttgatttct ttaatcgttg ccatcgaggt cttccaatgt atctaaggta tagttaccga
    14221 gtttgccatc acggacttcc ttcacgaaga gactgtaaaa acggtcatag tcatcacgga
    14281 aaccgagggc gcgggtcata tccataataa tcacaggcgc ttcttcttca attttcattt
    14341 gtttgaagcg ttcagccagc ttttctggat aatgttcttt gaaataattg ataccaaaaa
    14401 tggtaacctc atccatagga agcaactggt ctttgatagc tccagtcaat gccaacttaa
    14461 gtgcaacagt ttcatcctca aacttaggcc agagaatccc cggtgtatcc aagatttcca
    14521 ggtctttatt ggttttaagc cattgttgac cttttgtgac ccctggcttg tttccaacaa
    14581 cagcaatctt tttaccagcc aaacggttca tcagagttga tttaccagcg tttggaatcc
    14641 cgataatcat agtacgcaag gtttcaatct gaatcccacg ttctttctgg cgagcaatct
    14701 tatcagccat gagcttcttg gccgcatctg ttacaacttt tacagtcact tgctctttgg
    14761 agttgatagc tagcgtctgg attccttgtg attcaaaata ctgacgccat tccttggtca
    14821 ttgctggatc agccaagtcg gccttgttta aaatcaagag ttttggttta tcaccaacaa
    14881 tcttggtcaa cataggattt tgactagata gaggcaagcg tgcatctact aaaatcgtca
    14941 caaaatcaac aaattttaaa ttctcctgca cctgtcgacg agctttagac atgtgaccag
    15001 gaaaccattg aatagtagcc attgagtttt ttcctttcat agcaaggatt tagatcccct
    15061 attttatttt actattgttt gaacatagag agttttcaaa tgaatgatgt ccaaacagtt
    15121 cttgttttat gacatagaaa tctactttat attatatcat gccttcttta ttttgtccga
    15181 tgatagcctt ttaccctatc ttttacccta tatagaaaaa agccatcaaa tgatgacttt
    15241 tcagagggag caaaaaactg ttcccgtcat attatgaacc aaaagaacta aactaccaac
    15301 agctaatagg cctaaagctg taagctcaga agttgatgaa cctgttgcag gaagagtttc
    15361 ctgctgataa ataagatcat aacttacttc attatcagta ggaattcctg cttgaatctt
    15421 ggtttgttct tcatcacctt tttaaacttt atttcctaat agtagacact attttttatt
    15481 ctcaaaaatt gaacttctaa aatcatttgt ttggtcaaga taggctttaa agattgctct
    15541 cttgagcttg tggacttcac tatctatgtc tggattgtag ttattccaat ggtagtaatt
    15601 gtgttctgca tccttacgaa cagcaacgtc cattaatttc tgtaattcat ctacactgct
    15661 tgtagttttc ttggcaaatg tttgccaagg ctgtgttgta tcattaaaag taactttgtt
    15721 caatctatca aactgatctt gtcgttcttg atacatagct ttcttaaact cactccaagt
    15781 atgatattgg ttattaaata ccttttccaa aaccaagtca tctgtaacca aacctcttgt
    15841 tttaccgtag agattgattg tcttgccctt ttgtttggct tcttcttcgt attggtttga
    15901 gatatatggt accataccat ctttaaagcc tttagcagct agtagttcat aggctatacg
    15961 acgtcccatc aggtcacctg gtgttcctat atcattactt aatgcagcat aaatcggtgc
    16021 aaataactta atagtgaagt agccatttct ttcgtattta cctgaggcat attccctaga
    16081 cgaaagaata ttattatcaa tcaaatcatt gaatgaacgt agtctttcaa cttcttctac
    16141 tgttagattt ctgacaacat ttgttgcata gaccttattt ccatcaggat caggatggta
    16201 ctcatttgta acttttctaa gaagttgttg tttttgattc gtatccaatt taagaattga
    16261 atttccttcg agatattcca acatataaac aacgtcaaac atgttgtgga catattgctt
    16321 caaatcatct gcattattaa atcttgtagt tggatcaagt acttgtaatc gtcgactttc
    16381 tgtactatca gattttgaat gtttcaagat ggagttgatg gtaatggtcg catcatctgg
    16441 atggtctggt gcttgtaata atcctttagc aaagaactct ggtcccaagc cacttcttcg
    16501 accatatcct ccaagataaa tgtcctgatc tgagtcatgt gtcatctcat gcgtataagt
    16561 aatagctcca tccttatcca acattcgata acccatataa taaactgcat cacctgtagc
    16621 ataagcaccg tgttgattat gcccaacttt atttccaaca ggtccaaaga aatgttgcat
    16681 tgcaggattt ggattatcaa aatctgccac ttctgtagct ttccctacgg tattatcatc
    16741 gccaaattta taagcatcgt aaagcaaaat atttctataa agtttttcac gtgcattgtc
    16801 gtctaaaata cgataccaat aatcgtagtg atctcgctga cgtttggctg tttcacgcgc
    16861 attttcttca acaaaatcat tgagagcctt gcccgcttta tggtcactac tgcggtagcg
    16921 atcataagct ccaaatccta gactagacat ggtcgagatg acaaatacgg atctctctgg
    16981 caaggtcagg agaggcaaga ccatattgcg gtatttccat gtggcactcg tgatacgatc
    17041 ataaacaccg atagaatact tggtgctagc taacccttgc ttcgttttca cctcttcgat
    17101 agtggatttt tcttcgacaa tgtaagcctt agtctctgat ttaaaccagt cattattgct
    17161 tgtatttggt aaaaagactt ttcggtaatg ttccagcgtg ctaaacaaat ctgtcgttcc
    17221 atgttgactg gcaagactga taccataagt atcgacatta ttcttagcta gaagattgtt
    17281 aaagccagat ttacccaact caatcagagt atctaatggt gaagcattcc ccttaccaaa
    17341 gaagtccaaa tggtacagaa ctaggtcttt gacattcacc tgaccatagc taaagttata
    17401 ccaccgttcc agataggtca agccaagtag caaggcttcc ttgttgcgtt tgattttatc
    17461 tacaagataa ccttcagtga ctgggttagc actagccagt ccagcatccg ctgacaagag
    17521 ttttttcaaa ctgtcttcca gttgttgttt tgttttggcg aactggtctt ctagatagag
    17581 ctcagtttgc ttgacgtttg gagaaatacc cagcgtcttt ctgatggctt ctgaatgata
    17641 gtcaaccttt tgtaagtcaa gtaagacttg cttgatgata gaggtttggt catacaggaa
    17701 ttggtttggc gtatagagaa gtccagtatt gcccagacta tattctgcta atttggcgaa
    17761 atcattctgg tatttgagat ccagcttctc agataaatca tccttgtagt gaagcaagag
    17821 tttgtttgca gtctgtttgt tagaaacaat gtctgtgatg acttggttgt ccttcatcac
    17881 gactgctgac aagagttctt tttgatataa aagactgttc tcattgacca gatttccgta
    17941 tttgacgatg gttgccttgt tgtagaaagg tagcaatttt tcaatgtttt tataagtcaa
    18001 gttgcgctta gcttgataat aggccacctt agaaaaatca ctgtcttttt tgccacttgt
    18061 tgaaagtggc tccactgttg gtagaatgag aggattgatt tctgcttttt tgcttgcaat
    18121 ttgagaagca tctagcattg ttcctctttc ttcaaaggat tccttgctga cgacctcatc
    18181 cttgaccaag gtgacattgt agactctgtt gtccttgctg ctgaatgtgt cctttacctt
    18241 catttcgtta tagtggtaac cagtgatggc atttccgttg gttacattaa catcgctgag
    18301 aacattggtc aaacttccag catgcctaac atcaccagaa gttcgatccc acaaattgcc
    18361 tgccactcca gcgactctac caaagtgctt gacattgttg atatcacctt cagcatagct
    18421 atcttggatc tgtgcatctc ggtctactag gcctgcaagt ccacccacag tctgatctga
    18481 agtatttgtg ttagatgaaa tggccactgt cgcttttgac ttagtaagta aagccttgtc
    18541 acctgtcaaa tgaccgacca taccaccgat attgtaggca gcagtcgttt cataggtatt
    18601 gacaattctt cccttgaaac tactctctgt gatgcttgat tgatctgcct tagccaacaa
    18661 accaccgata ccacgttcac cggcaagtac accatccaca tgaacttgct taatttttgt
    18721 gttattctga gcttcatttg ccagtgaacc gatatcatct ttccctgaaa tagagacatt
    18781 tttcagactc aacttttcta ttgtggcccc tctcaagctt tcaaaaagag gctttttcaa
    18841 gttgtaaata gcataatgct taccatcttt ttcaccaatc aactgaccag taaaggttcc
    18901 cttgatatag gatttatcat cagtactaag ctccacttcg ttagcattca ggctagctcc
    18961 taaatgatag gttccagaga gattttggtt tatagctttg accagattac taaaggaagt
    19021 aaagtttgtt gtttcttctg ttcccttctt agctagatag aaggtaaaat tatctttata
    19081 tctgctttct atctcctgct gaagcttctc tacttttgct gtgattttat aaaggatttt
    19141 atcatttttt ctttcctctg atattgatgc tactggtagg tatacatctt tgaatgaaga
    19201 agatttcact ttaacaaagt agctatttgg attgcttgga acttgctcta acgaaatgtg
    19261 ttgtttataa gtaccatttg acaaactgta taactctagg tcggaaacat ttcttaattc
    19321 aagtgttttc tctggttctt ctaccttttt atcagggtct agttcatttt cttgtttaat
    19381 ttcttcgttt ccatttgaat tggatgtgtt tgattcggtt gaaacatcct cagttgaatt
    19441 tccgtttgat ggttctggtt ctgtttgtcc attctctgat gttgtattac ctgaattttc
    19501 tggttttgtt gcagttccgt ttttttctgg ttgatttgat tctccaactg aggttgtcga
    19561 atcactagga ttactggaca cttccccagt attttcgcta gatgtatctg gtgatacttt
    19621 ctctgaattc gttgttgatt cttctgcagg ttgaactgga ttttctgctc cttgaattga
    19681 ggttccttct gtagtacctt catttggatt tactggtgtt tcttctgttg gttttactgg
    19741 aacttcttca gttttttctg gaccttgttc tttggtcttc tcaaccggag tttcaggttt
    19801 tacttgctca atattaccct tatattttgg caatggggct acctgctctg gttcaccttt
    19861 atcacttacc acagtatctg gcgactctgg ttgaacctca gtctcacctt tgtcggtcac
    19921 aactgcttcg ggtaatgtcg gttgaacttc tggttcgcct ttatcactta ctacagcttc
    19981 gggcaactca ggctgaattg cgggttcaac aatagctcca gactgtacgt ccttatgttc
    20041 tacaccagtc tcaggttgtt cctttataac ttgagttttt ttagtacctt tttcgactat
    20101 tcttggacta ggcgcagtcg ttgaagttga aacaatttct cgcgaaactt cttccttgtt
    20161 tacagagaat attctgacga tttcaacttt cttacctaat ttaccttctt gttttactct
    20221 tacagttcct tcagctaaat caggattttc ttgaatttct tcttgaaaat ctatttttgt
    20281 ctctatagtt tcctcatgat ataagagttc aggtttgttc aattgacctg ataaaacttc
    20341 atcctgtgga tttaatgtat ttaccccagt cttttctttt ggagaaatct tctcctcttt
    20401 cttcgtttct agattcttat gttcggctaa ttgttcttga gaatctgaag attgtttctc
    20461 ttcttttctt ggattgatta attcagtaga gaaaggtttt tcaactactt gaacttctgt
    20521 cggcttagtt gaagaaacag gtgtttgttc ctgaatagct tgtactgttg atggatggtc
    20581 tacaaaattt ggtgtaacat tataatccac cttttgttgt tttgtaggag tggctgctga
    20641 tttttcttga ttacttactt caaaatcaga agtcgttttt ccctctttga tatatccaat
    20701 ataagtgtaa cctgaaatct ctttaggaag aggtaatttt tctccagagg tcaattcata
    20761 gtccgtattg taatttagca aaagatgatt ttctaaagca tggactgaaa ctaagacacc
    20821 atttcccatt cccgcaacca atactaaatg taataccgtt ttattcttaa cctttttctt
    20881 ggaaacagca aaaattaaaa ttcccagagc agctaagcta gcaccagcaa ctagggcttg
    20941 cctctcattc ttgcttccag tatttggtaa ttccgccagt tgattttgag aatttaactt
    21001 ataaacaaga taataagttt catcatcatt ctccacgtat gtcggaatat catagacaag
    21061 ctgcttcttt tcttctgatg atagctctga atctgccaca tatttatagt gaactcccgc
    21121 agtttcttga gcatccacag atgaactagc taatacagac ataaaaaata aacttgaaat
    21181 cgttgcagat acaagtccta ctgataattt tctaaatgaa aaacgctctt gtttttcacc
    21241 aaaatacttt tccattattc ctccttgaaa taaaatttat atatgttaca aagaccttta
    21301 ttatattagt gtattatcta ttatctatag aaaaggcagt ataccttaat tatactctta
    21361 atttacaaaa aagtcttaaa attgagatgc gctttcatac tttgttttat attatttggg
    21421 ggtacaataa cacctaccat gaaatttaca cggtaggtgt tactcatatc actaatcgtt
    21481 ctaaaaatgg tttgaggcag ttgaggagaa ttccttctat ccagcttcct tgtgctgatg
    21541 agcgatggtc ttcctgcagg ctttttttta gaaaatctcg gacttgttct ggtgcgattt
    21601 caaattcaaa ggctttcatt ttatagaaaa agtcgatgag atgatctgac aggtattcag
    21661 ttgaaaaggg tacttcacca ctttttcgat attctaataa gagtctagaa aatcgagctt
    21721 tttcttcagg aagctcacga aaataggaat tgaggatcca agtctgcttc tgttttcttt
    21781 caattggatc ctgactggca attcgttggt ctttttccag ctctttttgg tattgtttgg
    21841 ccttgatagc tcgttctgct ctatttttac caaaaagaat tttttcccac ttgcgttctt
    21901 cttgagtcag ggtctctgta aagccaaagt aatcttgata agcacgctct gcgggtccca
    21961 tggctagaac cagattgtct gcatattgct tggcgatttt atccctcttc ttgcgttctt
    22021 tctctgcctg gatacggagt tcttgttcgt agtcaatttt ctccttgcct agcttgacaa
    22081 ggtagagttg gtcatccgat ttcccaagta aaaagggttt gatacacttt tcaaggactt
    22141 cttccatccg agcctttttc tttggttccg ccttggtcca acttcctccc tgaaagactt
    22201 ctaggaaaag ctggtagtct ctctcaggcg caaattgatt gccacgattg ggtttgaaaa
    22261 cacctttttc ccagagccat tttagaagtc gctcgtcaaa gtcactttta ttgaccttga
    22321 ttttttcctt tttctgagct tttctggtta gattttcaac ctttctgagc agtttttctt
    22381 cctcttccaa ttgctggtca agggacaatc gatgaaaatg acgaacacag tcgctaccaa
    22441 ttggaaagag gcgttggcct gtgacaccgt taaagagttc ataagcgtat ttgatggcat
    22501 ttccacagac acaattgcta cggccgatac cgttaaaaat caaggaaact tcattccatt
    22561 ccttggtagc ttgttcccaa gtattcgctt tcgaagcctg taaaactgca tcgtgcaggg
    22621 attttctaac tggaagtgtc atgaggtctc ctttctaata ctcaataaaa atcaaagagc
    22681 aaactagaaa gctagccgca gtcagctcaa aacactgttt tgaggttgta gat
//
LOCUS       DAVMHG010000019        21877 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMHG010000019 DAVMHG010000000
VERSION     DAVMHG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.019"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            131..868
                     /locus_tag="VYR08_000588"
     CDS             131..868
                     /locus_tag="VYR08_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466160.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            865..2556
                     /locus_tag="VYR08_000589"
     CDS             865..2556
                     /locus_tag="VYR08_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3466161.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            2672..3235
                     /locus_tag="VYR08_000590"
     CDS             2672..3235
                     /locus_tag="VYR08_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV3466162.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            3257..8920
                     /locus_tag="VYR08_000591"
     CDS             3257..8920
                     /locus_tag="VYR08_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV3466163.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGKSYIKGEFKGKLLGTNDNVRHSIFDL
                     EYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEG
                     RDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADIEV
                     HRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPYGA
                     IDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRISEE
                     EANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTKFT
                     PYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQK
                     RYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTEST
                     YQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAA
                     LRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGS
                     SGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQ
                     FEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSREK
                     RPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEW
                     VDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYG
                     ISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFER
                     KNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKV
                     EPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSY
                     IAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEG
                     HAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQ
                     EGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            8981..10702
                     /gene="pabB"
                     /locus_tag="VYR08_000592"
     CDS             8981..10702
                     /gene="pabB"
                     /locus_tag="VYR08_000592"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV3466164.1"
                     /translation="MHRKTVIDFRTFGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            10822..12348
                     /locus_tag="VYR08_000593"
     CDS             10822..12348
                     /locus_tag="VYR08_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3466165.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            12435..13354
                     /locus_tag="VYR08_000594"
                     /pseudo
     CDS             12435..13354
                     /locus_tag="VYR08_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            13489..14448
                     /locus_tag="VYR08_000595"
     CDS             13489..14448
                     /locus_tag="VYR08_000595"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV3466166.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            14545..15384
                     /locus_tag="VYR08_000596"
     CDS             14545..15384
                     /locus_tag="VYR08_000596"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV3466167.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(15432..15602)
                     /locus_tag="VYR08_000597"
     CDS             complement(15432..15602)
                     /locus_tag="VYR08_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV3466168.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            15723..16607
                     /gene="miaA"
                     /locus_tag="VYR08_000598"
     CDS             15723..16607
                     /gene="miaA"
                     /locus_tag="VYR08_000598"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV3466169.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            16600..17838
                     /gene="hflX"
                     /locus_tag="VYR08_000599"
     CDS             16600..17838
                     /gene="hflX"
                     /locus_tag="VYR08_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV3466170.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            17831..18454
                     /locus_tag="VYR08_000600"
     CDS             17831..18454
                     /locus_tag="VYR08_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV3466171.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            18469..19398
                     /locus_tag="VYR08_000601"
     CDS             18469..19398
                     /locus_tag="VYR08_000601"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV3466172.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            19419..20174
                     /locus_tag="VYR08_000602"
     CDS             19419..20174
                     /locus_tag="VYR08_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV3466173.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(20228..21136)
                     /locus_tag="VYR08_000603"
     CDS             complement(20228..21136)
                     /locus_tag="VYR08_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV3466174.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            21200..21469
                     /locus_tag="VYR08_000604"
     CDS             21200..21469
                     /locus_tag="VYR08_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV3466175.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            21469..21849
                     /locus_tag="VYR08_000605"
     CDS             21469..21849
                     /locus_tag="VYR08_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3466176.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         6937 a         3863 c         4879 g         6198 t
ORIGIN      
        1 gtactttgag aaacctgtgg ctatcttcct agtttacttt ttgattttta ttgagtataa
       61 gaggtctttc ctaagcagtt agaggagaat tttgctatac tgatactagt aagtggtaaa
      121 ggagcagagc atgacctaca caatcttaat cgtagaagat gaatatctgg taagacaagg
      181 tttgactaaa ctggtcaatg tagcagccta cgatatggaa atcatcggtc aggctgaaaa
      241 tggaaggcag gcttgggaat tgatccaaaa gcaggtgcca gatatcattt taaccgatat
      301 caacatgcct catctaaatg gcatccagtt ggccagtctg gtacgagaaa cctatcctca
      361 ggttcatttg gtctttttaa caggttacga tgattttgat tatgccttgt ctgctgtcaa
      421 actaggtgtg gacgactacc tgctcaaacc cttttctcgt caggatattg aggaaatgtt
      481 ggggaaaatc aaacaaaaac tagacaagga agagaaagaa gagcagttac aagatttatt
      541 gactaatagg tttgaaggaa acatggccca gaaaatccag tctcatctgg ctgatagcca
      601 atttagttta aagtctttag ccagtgactt aggttttagt ccgacctatc tgagttcctt
      661 gattaagaaa gagttgggct tgccttttca ggattatctg gtgagagaac gtgttaaaca
      721 agctaagctc ttgcttttaa ctacagatct gaagatttat gagatcgcag agaaggttgg
      781 ttttgaagat atgaactatt ttacccaacg ttttaagcag attgcaggtg tgacacctcg
      841 tcagtttaag aagggagaag accgatgaag cgttcttctc ttttagttag aatggttatt
      901 tccatctttc tggtctttct cattctccta gctctggttg gaactttcta ctatcaatca
      961 agttcttcag ccattgaggc caccattgag ggcaacagcc aaacgaccat cagccagact
     1021 agccacttta ttcagtctta tatcaaaaaa ctagaaacca cctcgactgg tttgacccag
     1081 cagacggatg ttctggccta tgctgagaat cccagtcaag acaaggtcga gggaatccga
     1141 gatttgtttt tgaccatctt gaagtcagat aaggacttga aaactgttgt gctggtgacc
     1201 aaatctggtc aggtcatttc tacagatgac agtgtgcaga tgaaaacttc ctctgatatg
     1261 atggctgagg attggtacca aaaggccatt catcagggag ctatgcctgt tttgactcca
     1321 gctcgtaaat cagatagtca gtgggtcatt tctgtcactc aagaacttgt tgatgcaaag
     1381 ggagccaatc ttggtgtgct tcgtttggat atttcttatg aaactctgga agcctatctc
     1441 aatcaactcc agttggggca gcagggcttt gccttcatta tcaatgaaaa ccatgaattt
     1501 gtctaccatc ctcaacacac agtttatagt tcgtctagca aaatggaggc tatgaaaccc
     1561 tacatcgata caggtcaggg ttatactcct ggtcacaaat cctacgtcag tcaagagaag
     1621 attgcaggaa ctgattggac ggtgcttggc gtgtcatcat tggaaaagtt agaccaggtt
     1681 cggagtcagc tcttgtggac cttgcttggg gccagtgtca catctcttct tgtctgtctc
     1741 tgcttagtgt ggttcagtct taaacgctgg attgctcctt tgaaggattt gagagaaacc
     1801 atgttggaaa ttgcttctgg tgctcaaaat cttcgtgcca aggaagttgg tgcctatgaa
     1861 ctgagagaag taactcgcca atttaatgct atgttggatc agattgatca gttgatggta
     1921 gctattcgta gccaggaaga aacgacccgt cagtaccaac ttcaagccct ttcgagccag
     1981 attaatccac atttcctcta taacactttg gacaccatca tctggatggc tgaatttcat
     2041 gatagtcagc gagtggtgca ggtgaccaag tccttggcaa cctatttccg cttggcgctc
     2101 aatcaaggca aggacttgat ttgtctctct gacgaaatca atcatgtccg ccagtatctc
     2161 tttatccaga aacaacgcta tggagataag ctggaatacg aaattaatga aaatgttgcc
     2221 tttgataatt tagtcttacc caagctggtc ctacaacccc ttgtagaaaa tgctctttac
     2281 catggcatta aggaaaagga aggtcagggc catattaaac tttctgtcca gaaacaggat
     2341 tcgggattgg tcatccgtat tgaggatgat ggcgttggct tccaagatgc tggtgatagt
     2401 agtcaaagtc aactcaaacg tgggggagtt ggtcttcaaa atgtcgatca acggctcaaa
     2461 cttcattttg gagccaatta ccatatgaag attgattcta gaccccaaaa agggacgaaa
     2521 gttgaaatat atataaatag aatagaaact agctaactcc cagtcaattc tgggagtttt
     2581 atgcgtaatt gggcaaactt tctaggttgt ctatcttgct aaaaacaaga aaaaacggta
     2641 tgatagaaga gtgacaacag aggtaacaga gatgaacgag acacagattc aaagagaaac
     2701 acgtcaggta gtggaagatg ttttagaaaa gaccaatttg aagcaggggg ccctttttgt
     2761 tttaggtcta tcttctagtg aggtgctagg tggtcatatt gggaaagaat ccagtcaaga
     2821 gattggtgag ctgatagtgg agactatctt ggatattcta ggtagtagag gtatccattt
     2881 agcagttcaa ggttgtgagc atgtcaatcg ggctctcgtt gttgaacgtc aggtggcaga
     2941 gcagtttggt ctggaaattg tcagtgtcca tcctactctt catgcaggag gttcggggca
     3001 gttggcagcc tttaagttta tgcaggatcc agttgaggtt gaatttatca aggctcatgc
     3061 tggattggat atcggagaca ctgcaattgg catgcatgtc aagcatgttc aggttccgat
     3121 tcgccctatt ttgagagaga ttggtcatgc ccatgtaacg gcactggcta gtcgtccaaa
     3181 attaatcgga ggtgcgcgtg cgcactatcc gcaagatgct attagaaaga cctgagaatg
     3241 aataaaggag ataaaagtgt ttaaaaaaga ccgtttttca attcgtaaga ttaagggagt
     3301 tgtaggctct gtatttcttg gaagcctttt gatggctcct tctgtagtgg acgcagccac
     3361 ctatcactat gtaaataaag agattatttc acaagaagct aaagatttaa ttcagacagg
     3421 aaagcctgac aggaatgaag ttgtatatgg tttggtgtat caaaaagatc agttgcctca
     3481 aacagggaca gaagcatctg ttttgacagc ttttggttat ctatcaggtg acatcctcaa
     3541 aacgcttggc ttggacactg ttttagaaga aacctcagca aaacctggag aggtgactgt
     3601 agtcgaagtt gagactcctc aatcaacaac aaatcaggag caagctagga cagaaaacca
     3661 agtagtagag acagaggaag ctccaaaaga agaagcacct aaaacagaag aaagtccaaa
     3721 ggaagaacca aaatcggaga taaaacctac tgacgacacc cttcctaaag tagaagaggg
     3781 gaaagaagat tcagcagaac cagctccagt tgaagaagta ggtggagaag ttgagtcaaa
     3841 accagaggaa aaagtagcag ttaagccaga aagtcaacca tcagacaaac caacagagga
     3901 accaaaagtt gaacaagtag gtgaaccagt cgagccaagt gaagacgaac aggcaccaac
     3961 ggcaccagtt gagccagaaa agcaaccaga agctcctgaa gaagagaagg ctgtagagga
     4021 aacaccgaaa ccagaagata aaataaaggg tattggtact aaagaaccag ttgataaaag
     4081 tgagttaaat aatcaaattg ataaagctag ttcagtttct cctactgatt attctacagc
     4141 aagttacaat gatcttggac ctgttttaga aactgcaaaa ggtgtctatg cttcagagcc
     4201 tgtaaaacag cctgaggtaa atagtgagac aaataaactt aaaacggcta ttgacgctct
     4261 aaacgttgat aaaactgaat taaacaatac gattgcagat gcaaaaacaa aggtaaaaga
     4321 acattacagt gatagaagtt ggcaaaacct ccaaactggg gttacagaag ctgaaaaagt
     4381 tgcagctaat acagatgcta aacaaagtga agtaaattct gagactgcaa gtcttaaaac
     4441 agcaatttct agattaaata cagataaagt tgaactcgaa aatcaactta agattgcaca
     4501 gggaaaaact gaaactgatt tcagcatgga atcttggact gttttgagta cagctaaaaa
     4561 taaagctcag gaagtaaaag ataacggaac agcgacccaa gaacaaataa acgaagcaga
     4621 gaaaagtttg aaaacagccc ttgctgactt atctgttgac aaaacagctt tgggaagcgc
     4681 tatcgataca gctactaaga aaaataaaga gaattataca aatcaaacat gggcagagtt
     4741 ggaaacagcg cttacagcag caaaatctgt aaatactaac gagtctaaac aaagcgacgt
     4801 taacgaagct gctgaaaaat taactgcaac tatggaaaaa ttggttgaat tgtctgaaaa
     4861 gccacgtttg actctatcta ttgaaaagag agacatagat cgaaaagcta cggtcacgta
     4921 tactttggaa aatccagcaa atactcaaat aaaatcaata actgcaactc tgaaaaaagg
     4981 agaggaagtt gttaaggatt ttgttttaac agaagaaaat cttaaaacta atcatttaac
     5041 tgctttgttt gaaaagctag actactacaa agaatacact ctatctacgg atatggtata
     5101 taacagagga aatgatgatg agacggagag tatttcagaa gaactaattc agctgaatct
     5161 taagaaacta gaattgaaag atatccagac agtctctctg atgaaatttg aaaatggtca
     5221 agaaagtcag gttacacact tgtctgataa accgaccgat ttgagtaaat tatacttaaa
     5281 agttacatca tcaacgtcta aggatgctgt gttagccgtt agctctatag aagaagagat
     5341 tattgaaaat aaaaaaatct ttaaaattca tgcagataca ccagaattag tagttcgtaa
     5401 gaaagatggc agtctttcta agggatttga ctattacatg gagagagtta tccctcatga
     5461 tggtgatatt tattatgatt ttaaagactt gatttcagct atgacatcta atcctacagg
     5521 tacgtttatt ttagggagag atatttcctc gcgtaatgta aaaccggatg gaaaatctta
     5581 tatcaaaggt gaatttaaag gaaaactact tggtacaaat gataatgtta gacactcgat
     5641 ctttgactta gaatatccat tgtttgatac tataaaatca ggagtcgtga aagatattga
     5701 tttcaagcat gtcaatatgg tattcccaga tagcaatcaa ggagataacg tagcaacaat
     5761 tgcaagagta atcaaagata agacaaaaat tgagaatgtt aatgttgaag gttatctcga
     5821 gggtcgagat catgtagctg gtttagttaa taatcttgaa ggtaattccg aaattgaaaa
     5881 tgtatccttt acaggaaaga ttaagtctaa aggtggtaat tctattacag caggtattgc
     5941 aggtagaaat atcttatctc gtgttaaacg tgcctatgta aatgcagata tagaggtaca
     6001 tcgatcatct aattctagca tgcttgttgc agtaaatgga ataaacgcag atgcaagtgg
     6061 tggttgggga acctggggta gacttactga gtcagttgct aaaggaacat tagaaaccaa
     6121 gcaaggaggg caagctggag gagcctcttc tacagtctgg ccttatggag caattgataa
     6181 tgttgtcagc tatgcaaaag tgaccaaagg gaaagaatta tttggatcag atggtgattt
     6241 aaattatgat tggtttatga aaaaaattag taatattttt ggtgttcaag gcatcagctc
     6301 aggcgattcg ggtagtgatt ctaaatttac gcgtatttct gaggaagaag caaatcaaaa
     6361 agttgcttct tataatatta cagctcccaa cttgatgagt gactcaagtc ttttggtaga
     6421 cagactcaat gaaagttgga aaaatactga tcaatttgaa agtatacagg attatcaagc
     6481 ccaaaaccaa ttaatttacc agaacttgac taaatttact ccttattaca ataaagaatt
     6541 tattgttcat gaaggaaatg ctctcactcc tgaacaggaa atattgaaga ctaagaaaat
     6601 caaatcgatt gtagggttga aaggaacaga gttcgttgta gatggcagtg atattgatac
     6661 tatcatgctt cattttgagg atggtagtca gaagcgatat aaggtaacct ctactggtaa
     6721 gttttcgata actaatttac cggaatatca ggttgaagat ttaaatgtgg tgtatacatc
     6781 agaacatata gtacatccac tagattcgtc actgataaat aaccttgttg aagaattgaa
     6841 aaaagtagaa ttgtatacag agtctactta ccaagttcta ggtattgata aagataatgc
     6901 taacaaattg aatagaacta agagattatt cttagatgag tcattagatg ctgttaaaac
     6961 acaattgcct acctttgtaa aaacaatgtt tgaaaatgag tggttgcaca tcaatggaga
     7021 atctagtgga gctgtcgctg ctttacgaca aaaaatcatg gataataaga cggctatatt
     7081 gcttgctttg acttatatca atcgttatta tgatgttaaa ttctcagact ataatattaa
     7141 aaaactgatg ctctttaaac caactttcca tggtgaaaag attgatctat tagatcgatt
     7201 aatccgacta ggatcatctg gagaaaatag attaaaaggt tcagaaaatg cagaaacgtt
     7261 caagcagtta tttgctagcg aaacgaagca gaaggatttg gtgacttatt tagactacaa
     7321 tcgctctttg ttgactaatt atcaaacaac aggggaatgg tttaaagaaa cgactaagga
     7381 ttatattcaa tttgaggaac gtccatcctt agtagaggag attaaagatg ccaaatatcg
     7441 agtttacgat aatttaaccg caccgtatta tcaaggatat atcctacctc tcttaacgtt
     7501 gaagaatact catttagcaa ttttatcgaa ctatagtact atgacatttg tcagccgtga
     7561 aaaacgccct aattggaaaa atgaagattt tgataagtgg gtgaaatatg tagcaactgc
     7621 tcatagaaac catgttgata cttggtataa gattttacct gataatatca aaggtaaaat
     7681 ggttaaagag aatgttactg ctgtttggga aggattgagc atcccaggaa gtgaatgggt
     7741 agaccagaat gctgtagata gaaaaggaag agattacgcc ccagctcgag aattctttaa
     7801 tcttgtaggt ggtcctatgg gaggttggta tgcttaccat ggttatggtg cgcatgctgg
     7861 aggaagaaat cgggttaatt atgaggtgtt tgatgtatta agtgagtatg gtatatcggt
     7921 ctttactcat gaattgactc acgttaatga tacttggatt tacttaggag ggtatggtag
     7981 acgtgaaaat atgggacctg aagcttatgc tcagggatta ttccagtctc ctgttccagg
     8041 tcagccgggt tggggggcgc ttggtctcaa tatggcattt gaaagaaaaa atgatggtga
     8101 tctaatttat aatgcctccc caactcaatt tgaaaatagg aaagagttag atagttatat
     8161 gaaaaattat aacgatactc tcatgatggt ggactacttg gaaggagatg cagttatctc
     8221 taaaggtaaa gaagctatta ctaaatggtt taaaaaagtt gaacctaagg ttgttagtca
     8281 aacagctcag tatgatacgg ttcgtcagtt gactgctgaa gagaaagaaa aactttctgt
     8341 ttctagtgtt gatgatttag ttgatcaagg attaatgtca gacagagctg taggaaataa
     8401 tacctataat ccagctgatt ttgaaacaag ttatattgcg atagattata tgactggtat
     8461 ttatggtgga ggaaagaata gtgtaggttc accaggtgcc ttgatgttta aacataatac
     8521 tttccgtatg tggggctact atggttttga agaaggtgtt ctaggatatg cttctaataa
     8581 atttaagcaa gcatctagag atgaaggtca tgctggactt agtgataact ttattatttc
     8641 taagatttcc aaaggggaat ttttaactat ggaagcattt aaaaagggat attttaagaa
     8701 agtagtagaa gagttaaaaa ctaaaggtat tcgtcctgtt actatcaatc aaaaaacata
     8761 tagcaccttt gaagaattgc aagaaggatt taagcaggct gttgaaagag atttgaagaa
     8821 aaatcagtta gatgagaggg aaacaagaaa cttcaaattc caagttttca gacaacttct
     8881 tcaacaaaca gatagcttta aaacttctat ctttagataa tcgaaatctc tcatctgctt
     8941 tgcggatgag ggttttctta ttttatgcta tacttaaagt atgcatagaa aaacagtgat
     9001 tgattttagg acttttgggg agagatatac ctttacccag cctattaaag agttgaaaac
     9061 gagaaatgta gaagaagtgg cagatttgct ggcacaagtg gaaagctacc aagagcaagg
     9121 ttattatgtg gtggggtatg tcagctacga ggctgcacct gcttttgagg aaaaattagc
     9181 agttcacaag gatcccctac tgggagagta cctgctttat tttactgttc atgatagcgt
     9241 agaaacttcc cctattcctc tgacttatga ggatattgat ttgccctcaa attggcagga
     9301 agtaacgtct gcagcagact atgaaaaggc cattgcccaa attcaccatc atttacggca
     9361 gggagatacc tatcaggtca actacaccgt ccaacttaag caaaagttaa atgccaatcc
     9421 ttttgccatc tacaatcgta tggtggtaga gcaggaggcg ggctacaatg cctatgtgga
     9481 acatgacgag atggcagtga tttccatgag cccagagctc ttttttgagc aaaatgatcg
     9541 cgagttgaca acacgaccaa tgaaggggac gactcagcgt ggggtaactg accaagaaga
     9601 tcttgaacag gccagttggt tggaacagga tcccaaaaat cgctctgaaa atatgatgat
     9661 tgtggacctc ttgcgcaatg atatgaaccg tatttctgaa gttgggagcg agcacgtgga
     9721 gcgtctgtgt caggtagagc agtattcaac tgtttggcag atgacttcga ccatcaagag
     9781 tcagttgcga gaggatgtgg accttgttga aatcttccac tcactctttc cttgtggttc
     9841 cataacgggt gcaccgaaaa ttgcgacaat ggagattatc aaggacttgg agcctcaacc
     9901 gcgtggagtc tactgtggaa cgattggtct cttgcttcca aatggacgac ggatttttaa
     9961 tgtggccatt cgtaccattc aacttcacaa aggtcaagcc atttatggag ttggcggagg
    10021 gattacttgg gatagcactt gggaatctga ataccgtgaa gttcatcaaa aggcagcagt
    10081 tctctatcgt aaacaagctc gcttccaatt aattacaact ggaaaaatca gccaaaaaca
    10141 actgatgttt gaagaacaac atctggaaag gctgagaaaa gctagtcggt attttgctta
    10201 tccttttgac ccagaaattt tgagacaaaa gattgaagca gagtgtcaga cttgtgatgc
    10261 taatcaagat taccgcttgc gaatttctct cagcaagtct ggagagatag aattcagtcg
    10321 tcaaatctta acacctctta gtccaagttt ttgtcaggcc aaactttgtc tgcaagaagc
    10381 gaatttgaat caatccttta cctattttaa aacgacttac agaccgcatt tgagcataga
    10441 ggaacaagag aagatttacc ataatggaaa aggagaactt cttgaaacct ctatcggaaa
    10501 tttggttctg aaaatcgatg ggaaactcta cacaccgcct atccgacttg gaatcttgcc
    10561 aggaatttat caccagtatt tactagaaac aggacaggta gaagagaaag tcttgacctt
    10621 ggcagatttt gcccaagcag aagctattta cggctgtaat gcagtgagag gcttgtatga
    10681 gttgcaagtg ataggattgt gagttttagc tattaatagt ttttaatata tattaaaata
    10741 tattggtgtt tacttttgat gagatatcat atttgtgtta taatgtaacc gtaaacaata
    10801 gaaatttaaa ggagattttt gatgaaatca aaaatagttt tgggagcttc gctagcgatc
    10861 gcaacactta gcttagtatc tcttgtcgag attgaaggac tctcaccgtt tttaatagag
    10921 aatgttagcg caaatactca ttctgcaaat aaagttatta atcataaagt aagtatttac
    10981 ttagaaaatg ctgatgaagg taaaggtcta actgtaaact tttcaacaga cagtgttagt
    11041 ccaaatttat ttgatgagtt cgaaaaaaag agtggaatta caataactac tatgttagtg
    11101 aatgctaaga caggtgaagt tgtagaaaaa agattaacac caagcgtttt tttacgttcc
    11161 aatgatttaa ccagtgggac catatcttcg tttattttta gtgaatatcc tgatggagaa
    11221 tataaatatg ttgtgtctaa aggagacttt atcgatccta agactcagtt taaacatcag
    11281 tatagaggag aatctccagt ttttagaata cgtaatcgaa aatatgtgga gttgggaaca
    11341 actgataaaa aattagatga aagacgtgat aatagcgttt ataaagatgg ggttgttgaa
    11401 cacaaggtaa atttaagttt aacaagttat caaggaggta atggagttac tgcaattttt
    11461 tcaactgata gtgttaactc taacttactt aattcttttg gtgaaaaggc taaaaaagtt
    11521 ttgattaggt ctaagttaat taatgttaag actggcgagg ttattgatga aacgttttct
    11581 ccaaaagtaa gtttaacctc taaaattttg aaaagtggtt ctacagcagt attttatttt
    11641 atagatttaa ctgatggcga atataaatat gttgcgtatg aaagccagca atatactgat
    11701 cctcaaacaa cattgactca tcagtataga ggagaatctc ctatttttag tatcaaagat
    11761 ggaaaatttt ctggtttagt atctgcctca aaaccagacg aaaacccgaa gccaacgcca
    11821 aaaccggatg agaaaccaaa accttcagca ccacaacagg agaaaacaaa acctacagtt
    11881 cagtctggtt gggtaggttc atcttattat caaaatggta aaaaagtcac tagtaagtgg
    11941 atttttgata aaaaatataa ttcatacttc tatctagatg catcaggaaa ttatgttcaa
    12001 aacgcttggg taggtaacta ttatctcaaa tcaggcggat acatggctaa aagtgagtgg
    12061 atttatgata agaactatgg ttcatactac tatctaacat cagaaggcag ctacgctcgt
    12121 aacacttggt caggcaatta ttatttgaag tcaaatggta agatggctaa aagtgagtgg
    12181 gtatacgata gtaactacaa atcatattac tacttgacat cagaaggaag ttatgctcgt
    12241 aacacttggg taggcaatta ctatcttaaa tcaaacggta aaatggctgt taatgaacgt
    12301 acaccagatg gttatcgtgt agatggttca ggtaaatggg ttaaataaga aactaaaaaa
    12361 taggataata ggtaatatca gatgaataaa aaaaattatt ttcatgtgct ttagcatttt
    12421 caacgattgg aacattgtca acgattgata aatcaggagt tcttcctttt tcagcgcaaa
    12481 cggtgcaggc tgacatacaa acgcttaccc caatggcaaa atattatatt attcttccga
    12541 aggatgcaga aatatataaa acttggagag gtactgtcaa tattcccatt attgatgcta
    12601 caaaaactac tccagaatta tcttatttca aagaagatca tcgcaactat atagctaatg
    12661 agaataagtc tggtgccaat tacatagagt ggaagggaac agtagaagag tttaaagagg
    12721 ccattaagaa gttgactgat aaaaaatcaa ctactgcaac accaaaaaag gatgaaaagc
    12781 caacaccaaa accggatgag aagccaaaac caacacctac agttcagtct ggttgggtag
    12841 gttcatccta ttatcaagat ggtaaaaaag tcattagtaa gtggattttt gataaaaaat
    12901 ataattcata cttctatcta gatgcatcag gaaattatgt tcaaaacgct tgggtaggta
    12961 actattatct taaatcaggc ggatacatgg ctaaaggtga atgggtttat gatgccacct
    13021 atcaagcttg gtattatttg acatcagatg gttcttatgc ttacagtaca tggcaaggaa
    13081 attactatct aaaatcggat ggtaaaatgg ctgtcaatga atgggttgat ggtggacgtt
    13141 attatgttgg cgctgacgga gtttggaagg aaggtcaagc aagtacagct tcttctagta
    13201 atgatagcaa tagtgaatat tctgctgctt taggaaaggc aaaaagttat aattcgttat
    13261 tccacatgtc aaaaaaaaac gtatgtatag acaattaact tctgattttg ataaattttc
    13321 aaatgatgca gctcaatatg ccattgatca ttagatgatt aacagatact ggtaggcgaa
    13381 aaaattcctt ataattcatt tacgcaaacc tttgcataag tgaatggatt ttgatatact
    13441 agtactgtaa gcattttcaa ttactacaca atgaaaaagg agaatatgat gagtcaaaag
    13501 attattggga ttgaccttgg tggaacttct atcaaatttg caatcttaac aacagcagga
    13561 gaaatccaag gaaaatggtc aatcaagacc aacattttgg atgagggaag tcatatcgtt
    13621 gatgatatga ttgagtctat tcaacatcgt ttggacttgc ttggattggc agcagcggac
    13681 ttccaaggca ttggaatggg atcaccaggt gtggttgacc gtgacaaagg gactgttatc
    13741 ggtgcctaca acttgaactg gaaaaccctt caaccaatca aacaaaagat tgaaaaagct
    13801 ttgggcattc catttttcat cgataatgat gccaacgtag cagctcttgg tgagcgctgg
    13861 atgggtgctg gagataacca accagacgtt gtctttatga cactcggtac tggtgttggt
    13921 ggcggtatcg tcgcagaagg caaattgctt cacggtgttg ctggtgcagc aggtgagctt
    13981 ggtcacatca ctgttgactt tgaccagcca atctcatgta cttgcggtaa gaaaggctgc
    14041 cttgagacag ttgcttcagc aacagggatt gtcaacttga ctcgtcgcta tgccgatgaa
    14101 tacgaaggcg atgcagcctt gaaacgcttg attgataatg gagaagaagt aactgctaag
    14161 actgtctttg atctcgcaaa agaaggagac gaccttgctt tgattgttta ccgtaacttc
    14221 tcacgttact tgggaatcgc ttgtgctaac attggttcaa tcctaaaccc atcaacaatc
    14281 gtcatcggtg gtggtgtgtc agctgcggga gaattccttc tacaaggtgt tcaaaaagtt
    14341 tacgatgaaa atagtttccc acaagtacgc acatctacta aattagctct tgcaactcta
    14401 ggaaatgacg ctggagttat cggagcagca tcacttgtat tgcaataaga taatgagaga
    14461 ggaaaatgct aacctagagt tagtaatgtt cctcttttat tttttatgct atactagtta
    14521 ggacaatgaa tgaggtgaga gtaaatgaca aaagcagata cgatttttaa agagaatatt
    14581 gaatgcattc taaaggaagg tgttttctca gagcaagctc gtcctaagta caaggatggg
    14641 actgttgcta attccaagta tgtaactggt gccttttccg agtacgactt atctaaagga
    14701 gaatttccta tcacaacctt gcgtcccatt gcaatcaaat ccgccatcaa agaagttctc
    14761 tggatttacc aagaccagtc aaatagctta gaagtgctta atgacaagta caatgttcac
    14821 tactggaatg actgggaagt tggagacacg ggaaccattg gtgagcgcta tggtgccgtt
    14881 gttaagaaac acgacattat caataagctt ctcaaacagt tggaaaccaa tccttggaat
    14941 cgccgcaata ttatttcgct ctgggattac caagctttcg aagaaacaga tgggctgctc
    15001 ccgtgcgcct ttcagaccat gtttgatgtc cggcgtgttg atggggaaat ctatctggat
    15061 gcgaccttga cccagcgctc caatgatatg ctggtggccc accacatcaa cgctatgcag
    15121 tatgtggctt tacagatgat gattgccaaa cattttggtt ggaaggttgg gaagttcttc
    15181 tatttcatca acaacctcca tatctatgat aatcaatttg aacaagctca ggaattgctc
    15241 cgtcgggagc cgtcaaactg ccaaccacgc ttggttttaa atgttcctga tgggactaat
    15301 ttctttgata tcaaagcaga agattttgag ttggtggatt atgaccctgt taagccacag
    15361 ttgaagtttg acctagctat ttaaaagaat agaaaaaaga agttgagaat aatcccaact
    15421 tcttttgttt cttaacgtga tacgcggcga cgagctgctt ttttacgatt ttcttcgatg
    15481 aaagctgctt tttgctcttc tggttcgatt actttctttt taaatgcgta tactgcacct
    15541 gcaacggcag cgacagttcc tgcgacacct gttacaagac ctttagcgaa tcctttagcc
    15601 atgagtcttc ctcctttata ttctcaatca gccagcctcc tcaagaggtc acatttttct
    15661 gactgacctt tttgtgttat aataatagta acgaaaaaat gggaattttt caaggaaaaa
    15721 agatgaaaac aaaaataatt gtgattgttg gaccgactgc tgttggaaag acggctctag
    15781 ctattgaagt tgcaaagcgc tttaatggcg aagtggttag tggagatagc cagcaagtct
    15841 atcgaggact tgatattggg acggccaagg ctagtccaga agagcaggca gctgttcctc
    15901 atcatttaat cgatgttaga gagataaccg agtcttactc ggcttttgat tttgtttcag
    15961 aagctaagat gactattgag gatattcaga gccgtggcaa gctagccatt atcgccggtg
    16021 ggactggact ttatatccag agcttgctag aaggttacca cctaggtggg gagactcctc
    16081 atgaggagat tttagcttat cgagctagtt tggagccata ttcagatgag gaattagccc
    16141 atttggtgga gcaagcaggc cttgagattc cccagtttaa tcgtcgtcgt gctatgcgtg
    16201 ccttagaaat tgcccatttt ggtcaggatt tggaaaatca agagatcttg tatgaaccgc
    16261 tgattatctg cttggatgat gaacgtagtc aactttatga gcgtatcaac caccgagtgg
    16321 atttgatgtt tgaggctggg cttttggatg aggccaagtg gctgtttgac cattccccta
    16381 atgtacaggc tgctaaagga attggctata aggaactctt tccttatttc cgtggagagc
    16441 agacctttga ggaagctcgt gagagtctta aacaggcgac ccgtcgtttt gccaagcgtc
    16501 agctgacctg gttccgtaat cgcatgcagg tcacctttta tcagattgga gaatctggtg
    16561 tacaagaccg cattttaagc cagatagagg agtttttaga tgattgaaac ggagaaaaaa
    16621 gaggagcgag tcctgttgat tggtgtggaa ttgcagggta tggacagttt tgacctctcc
    16681 atggaagaat tggctagttt agcgaaaacg gcaggggcag tcgttgtaga tagctacaga
    16741 caaaaacgtg aaaaatatga ttccaagacc ttcgtcggct ctggtaagtt ggaagagatt
    16801 gcgcttatgg tggatgcaga agaaatcact actgtcatcg tcaacaatcg tctgacccca
    16861 aggcagaatg tcaatctaga ggaagttctc ggtgttaagg tcattgaccg tatgcagttg
    16921 attttggata tctttgccat gcgggctcga agccatgaag ggaagctcca agtccaccta
    16981 gcccaactca aatacctctt gcctcgcttg gttggtcagg ggattatgct cagccgtcag
    17041 gcagggggaa ttggttcccg tggtcctggt gaaagccaac tggagctgaa ccgtcgtagc
    17101 gttcgcaatc aaatcacgga tatcgagcgc cagcttaagg tggttgagaa aaatcgtgcg
    17161 actgtcagag aaaaacgttt ggagtctagc acttttaaga ttggtttgat tggttatact
    17221 aatgctggga aatcaactat catgaacatc ttgaccagta agacccagta tgaagcagat
    17281 gagctctttg cgactctgga tgcgacaacc aagagtattc atctgggagg caatctccaa
    17341 gtaactttga cagataccgt tggctttatc caagatttgc cgacagagtt ggtgtccagt
    17401 ttcaagtcaa ccttggaaga aagcaagcat gtggaccttc tggttcatgt tatcgatgct
    17461 agcaatcctt accacgagga gcatgaaaaa acggttctct ccatcatgaa agacctggac
    17521 atggaagata ttcctcactt gacgctttat aataaagcgg atttggtgga ggatttcacg
    17581 cctacccaaa cgccatatac cctcatttct gccaagtctg aggacagtcg tgaaaacttg
    17641 caagcattat tgctagataa gattaaggaa atttttgaag catttaccct gcgagtgcct
    17701 ttttcaaagt cctacaagat tcatgattta gagagtgttg caattctgga agaacgtgat
    17761 tatcaggaag acggcgaagt gattacaggc tacatttccg agaaaaataa atggaggtta
    17821 gaagaatttt atgactgata ttaaaacttt ggctctaaag tatggaggtt atacaagtct
    17881 ggacaaggtc tatctggatc agcttctagc tggtaaaaca gatcaggagc agttaacact
    17941 tattacacct ccgccaagtg ttgttaatgc ctactttgca gaactctatc agaaaaagag
    18001 tcctcaggct gcgacggatt attttgcaga actcagtcag gaactgaatc tttacaatac
    18061 tgagccaagt ttcaccctag aaaataagcc ttttattcgg cttaatctgt ctggtaaatc
    18121 ctttggtttt tgttatgaga gtgacggcct tggtcggatt ttctttgaaa atgaggagaa
    18181 aatctcggat gacttgctct ttgaaattgc gcaaattttc ccccatcaat tagtctttga
    18241 agagtctggt aagatttaca tgaagcctgt cgaggacgag gaagttatca gcctagaaag
    18301 tctcacagct ttgactgatt tggaaagatt ggctgatggt cgaaaacgtc tcaaaggcta
    18361 cagccaagag gatttattgc aagaagctgc tgctttttct ggcaaacgct atttccgatc
    18421 ggaaaaccgc acagccatgt tatatattga ttaattagaa agtatcgaat ggatattcaa
    18481 tttttaggaa cgggggctgg tcagccctct aaagcccgca acgtttcaag tctcgccctg
    18541 aaacttttgg atgagattaa cgaagtttgg ctctttgact gtggagaagg tacgcaaaat
    18601 cgcattctgg aaaccacaat tcgaccacgt aaggtcagca aaatctttat tacccatctg
    18661 catggagacc acatttttgg tttgccaggt ttcctttcta gccgtgcctt tcaggccaat
    18721 gaagagcaga cagatttgga aatctacgga cctcaaggaa tcaagtcatt tgtcttaacc
    18781 agccttcgtg tgtcaggttc tcgtctgccc taccgcattc atttccatga gtttgaccaa
    18841 gattctctgg gtaaaattct tgaaaccgat aaattcactg tgtatgcaga ggagctggac
    18901 cacactattt tctgtgttgg ctatcgtgtc atgcaaaagg atctagaagg gacgctggat
    18961 gctgaaaaac tcaaggctgc tggtgttccg ttcggcccgc tttttggtaa aatcaaaaac
    19021 ggccaggatc ttgttttgga agacggaact gaaatcaagg cagcagacta tatctcagcg
    19081 ccacgtccag gtaagattat cactatttta ggagacactc gaaaaacggg tgccagtgtg
    19141 cgtctggctg tcaatgcaga tgtcctagtt catgagtcca cttatggcaa gggtgatgaa
    19201 aaaattgctc gtaaccatgg tcactcaact aatatgcaag ctgcacaagt agcggtagaa
    19261 gcaggtgcca aacgcctcct actcaaccat atcagtgccc gtttcctctc aaaagatatt
    19321 agcaaactca agaaagacgc tgccacaatt tttgaaaatg tccatgtggt caaagacttg
    19381 gaagaagtgg aaatctagca gtcacagaaa ggataagtat gcctactatt ctcattaccg
    19441 gagctagcgg tggtctagct caagaaatgg tcaaactcct gcccaatgac caactcatct
    19501 tgcttggtag aaataaggaa aaattagccc aactctacgg aaattattcc catgcagaat
    19561 tgattgaaat tgatattacc gacgattcag ccctagaagc tctggtaact gatctttatc
    19621 tccgctatgg caagattgat gtcttgatta acaacgctgg ttacgggatt tttgagggat
    19681 ttgaccagat tgctgataaa gatattcacc agatgtttga ggtcaatacc tttgccctga
    19741 tgaatctgtc tcgtcacctt gcggctcgta tgaaggaaag cagcaaaggg catatcatca
    19801 acatcgtcag catggcaggt ctaatagcta ctggcaagtc tagtctttac tcagcgacca
    19861 agtttgcggc tattggtttt tcaaatgctc tgcgactcga acttatgccc tatggagtct
    19921 atgtgacaac agtcaatcca ggtccaatcc gaacaggatt tttcgaccaa gccgacccag
    19981 atggaactta tcttaaatcg gttgaccgct tcctgctaga accagatgca gtggctaaaa
    20041 agattgtcaa gattatagga aaaaacaaac gagaactcaa tctcccgatt ttgttgaacc
    20101 tagctcataa gttttatacc ctctttccta agctagctga taagttggca ggggaaactt
    20161 ttaattataa gtaaaaagaa ccaatgtgca ggttgttgct agcctacata ttggttctta
    20221 tcatttctca actatcaaac tgaacttctt ctaggagata gtctataaag cgttcgccca
    20281 tcttagatag gctggttttc tcatgctgga tatagaccag ctcgatcggg tcatcaatat
    20341 ccagtgggat agaaacgata ttgtctccgt ttaggttgct gttcaaaatc cctgtcgcaa
    20401 tggtataacc atccaaacca atcaagagat taaagagggt ggcacggtca ctgaccacaa
    20461 tggatttctt gtggtgttct tgagaaagaa tctcttctga aaagtagaag gagttgtgcg
    20521 tcccttggtc atagctgagg taagggaaat tctccaaatc agacagtttc accttgtctt
    20581 tctttgccag agggttggtc ttgctgacaa agatatgtgg ttgcgctgtg aagagatggt
    20641 gggctagcag gtgattgtca tccagcatct tggttaaaac atcacggttg taactgttta
    20701 agaagaggac cccgacctca ctgcggaagt tcttgacgtc gtcgataatc tcccaagtcc
    20761 gagtttcacg aaggaagagt tcgtatttct ccatatcgct tttcttgagc aaagagacaa
    20821 aggcattgac cacaaaggca tagtgttgag acgaaacgct aaatagttcg cggtgggcga
    20881 caggattttt atagcgttcc tccagaagct gggtctgctc gacaacctga cgggcataag
    20941 agagaaactc catcccatca cgggtcaagg tgattccctt gggattgcgg ataaagatct
    21001 caatgcccat ttcattttcc aaatctcgca ctgcattgga gagacttggc tgagtgataa
    21061 agagttgctt ggctgcctca ttcatggagc cagtttcgac gattttgata atataatgta
    21121 attgttgaat tctcatgttt ttattgtacc atacttgcct tagaatcgct aaagaagata
    21181 ggaaaataag gagcattgtg tgagtctctt cttgaaaaaa acacaaaatt ttgctagaat
    21241 gagtcttatg aaaacattct atgatgtgca gcaattcctc aaacgatttg gtattattgt
    21301 ttacatggga aaacgcttat atgatattga actgatgaag ttggaactat ctcggattta
    21361 cgatgcaggg ttgatggata aattagacta tctagaggca gaagcagttc ttcgcagaga
    21421 gcacaaggta gaattggatt atattgagaa aaatggagaa aagaactaat ggttacttgg
    21481 attttgtggg cacttatact agcaatgttg gcgtggatgg gctttaacta tcttcgtatt
    21541 cgccgtgcgg ctaaaattgt ggacaatgag gagtttgaag ccttgattcg tacgggtcaa
    21601 ttgattgatt tgcgcgaccc agcagaattc cacagaaaac atatccttgg tgcacgcaat
    21661 attccttcaa gtcagttgaa aactagtctt gcagcccttc gtaaagataa acctgtcctt
    21721 ctctacgaga accaacgtgc gcaacgagtt acaaatgcag ctctttactt gaaaaaacaa
    21781 ggtttttctg agatttatat cctttcttat ggcttggatt cttggaaagg gaaagtgaag
    21841 actagctaac cttatttcca aattagtcga atgatag
//
LOCUS       DAVMHG010000020        21624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMHG010000020 DAVMHG010000000
VERSION     DAVMHG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.020"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(131..355)
                     /locus_tag="VYR08_000606"
     CDS             complement(131..355)
                     /locus_tag="VYR08_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEV3466177.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(360..539)
                     /gene="asp4"
                     /locus_tag="VYR08_000607"
     CDS             complement(360..539)
                     /gene="asp4"
                     /locus_tag="VYR08_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEV3466178.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="VYR08_000608"
     CDS             complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="VYR08_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEV3466179.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QERLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="VYR08_000609"
     CDS             complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="VYR08_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEV3466180.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="VYR08_000610"
     CDS             complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="VYR08_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEV3466181.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEGPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="VYR08_000611"
     CDS             complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="VYR08_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEV3466182.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGSTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="VYR08_000612"
     CDS             complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="VYR08_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV3466183.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGVKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="VYR08_000613"
     CDS             complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="VYR08_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEV3466184.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRIQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="VYR08_000614"
     CDS             complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="VYR08_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEV3466185.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRIILPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     KMYLTLLIAVIQSLAVSLSLPVQSSYSVILVVLMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSL
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10579..11256)
                     /locus_tag="VYR08_000615"
     CDS             complement(10579..11256)
                     /locus_tag="VYR08_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3466186.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARYIVE
                     VFSKKISDCVLAGLDISVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11241..11483)
                     /locus_tag="VYR08_000616"
     CDS             complement(11241..11483)
                     /locus_tag="VYR08_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3466187.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDASTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(11483..12679)
                     /locus_tag="VYR08_000617"
     CDS             complement(11483..12679)
                     /locus_tag="VYR08_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV3466188.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVHSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12688..13908)
                     /locus_tag="VYR08_000618"
     CDS             complement(12688..13908)
                     /locus_tag="VYR08_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV3466189.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13939..16383)
                     /locus_tag="VYR08_000619"
     CDS             complement(13939..16383)
                     /locus_tag="VYR08_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEV3466190.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSIYLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTVMGPML
                     QELAKKYKNVKLYPEVKIDQFEKLKSQMDVYLDINLHNSTSEVVKEISSLNKPMLAFY
                     TSQNGNHGQHLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHVDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16393..17403)
                     /locus_tag="VYR08_000620"
     CDS             complement(16393..17403)
                     /locus_tag="VYR08_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3466191.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIS
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSNARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFMADSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(17432..18307)
                     /locus_tag="VYR08_000621"
     CDS             complement(17432..18307)
                     /locus_tag="VYR08_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3466192.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVVAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18347..19225)
                     /locus_tag="VYR08_000622"
     CDS             complement(18347..19225)
                     /locus_tag="VYR08_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV3466193.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLFSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILNDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(19222..21306)
                     /locus_tag="VYR08_000623"
     CDS             complement(19222..21306)
                     /locus_tag="VYR08_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3466194.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIITQTYKNIEIVVVNDGSTD
                     ASGEICKEFAEMDHRITYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIELDYVET
                     LYKKITEYQADIAVGNYYSFNESEGMFYFHISGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISACGKLYKARLFEQLRFDMGKLGEDGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     AMYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKCDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFVQEDK
                     ALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEIINQFARLGKTILSFENTKTYEAGQEVYAVDQV
                     QAMIEKLREISK"
     gene            complement(21367..>21624)
                     /locus_tag="VYR08_000624"
     CDS             complement(21367..>21624)
                     /locus_tag="VYR08_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3466195.1"
                     /translation="SASASTSASASASTSASASASTSVSNSANHSNSQVGNASGSTGK
                     SQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT         6916 a         4619 c         3318 g         6771 t
ORIGIN      
        1 tatctacaac ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt
       61 tgattttcat tgagtataaa aaagaaaact tcagaccaat ccacaaaagg tgaatatctg
      121 aagttttctt ttatttataa gtaatcacca taaaggttaa tagtagaata aataaagcca
      181 aactcaccaa taaagtgagc accttgacca aggtgttgct ctgccagtag cttggtttac
      241 caatattact agtggcatcc atggaaaata gttgattttg acggggttgt atagttacca
      301 atacaatcaa agcaagagat aggataatag ctaaaacaat tagtatttca atcatagatc
      361 tacctcaaaa ttcccagcaa agtaaagagc aaaccaatca gaatcattaa gcccagtaaa
      421 agtgaaaaag ttttactaat cttctcccct cgggtttctt tttccttctt gatgggtttt
      481 tgtttcaact cttccatgcg gccttcaacg tctttgtaaa ataaatcttc ctctgacatg
      541 ttagcctcct aaaacagttt gcatggtttc ctgatatctc accaagtcaa cataatttgc
      601 atgttggcct tgtttgccaa gtgcctgacg catttgatca acatctgaaa gggccaattt
      661 aatggtctca accaaagcag caacttcact actttcaaat agatggtctg gagcgatata
      721 aagtctattg tgcaccgtct gattaaagcc aagaatcaag agattgtgct caaaggcctg
      781 acgcactgcc tgtagcaact cattactgtg gtttatatcc aagtaaatat ccgacagttg
      841 atacagctcc tgaatcttct gtggactagc gttctggtaa aggaccacat taggatagcg
      901 aagcatgtct aagagcttag aagacatctc tgtcaccgct gcaatacgga aagtgacatc
      961 aggcaaggct cctgcgattg cttctacttg ctcaatctga tctgaattgg tcaagattaa
     1021 ggcatctcgt cttaggaaat tatcacgttt gaactggtaa tgataaccca agtgcacaaa
     1081 ctgatcatgg tatttctcgt cagttaactc taaagcgcgc tcataagtcg ccttatttgg
     1141 aatgatgatc ttcttagtac gcacattatc actttccaaa atcaactgca tattacctgg
     1201 aatggcatca tagagacgtt cctgccatac caagacatcc gagccagatt tatctggatg
     1261 atggaaggaa accaagaaag gagtcgctag agtattaaag ataagctgac tggtatctat
     1321 ttccaaatct tgcaaaaaga aggtgataaa ttcaacttta tttgcaaagt aacgcatgga
     1381 ctgaccaggc aaagtcaata agatatcacc cgtcacatgg ttttccagta aaacttgttg
     1441 gccattgaca tcttggtaaa ctgtcataat cggctcgcta tctgcgctat aagtggtttt
     1501 agcaaagcaa gcaccaaagc gattgtagtg gtcaacctga cgtactcgac cttctaggtc
     1561 tttccagtct acctgtttaa ccaagcgagc ctgcattcca tcaacataat gaatgacagc
     1621 cctctcctgc gtcacatctt caatacaagc agactgatta tctcctaaaa tttcccaaaa
     1681 atctgaaacg ggaacttggt taaaatagag aggttttcca tcctcgtaac ctagataata
     1741 ggtaaaagga gaaagcagac catcaggcag aaaaccatct gcatcgatga ccactccaag
     1801 ttgagaaaga ccagtagcga ctagactttc atgtaaatct cgactttctt gactgtaact
     1861 atcataaagt tcaatcatgg agcacctcct ctactgtttt cttccacttt tctaaaattt
     1921 ctttggtcaa gaagccttct gcaatttggt aagaataggc acgcatagct tccaaacgat
     1981 tttcttgata caattgacaa atcttagcgg cataagcttg cttgatttgg tcttctacat
     2041 ggtcagatga acttggaatc aaataaccat tttgcccatc ctctataaag gtctgattac
     2101 cataaggcac atcaaaacca attagaggta gacctgaacc aatagcttcc atcaaggtca
     2161 gaccaaatcc ttcgctggta gaagccgtta agtagacctc atactggcta taaatctgcg
     2221 aaagttccgc atgccccttg agttggatat agtcctctgc ctgatgattt gcaataatgt
     2281 ctctaagcag agaatcttct ccaccactac catagatatc aaaggttagt tccggtaact
     2341 ccttatgagc ttcaatcaca gctttcacaa gccaatcaat gtgcttttct ttggcaagac
     2401 gtgaagccgt aatcaatgaa aatggtttgc gcccttgact tgaatctgtc aaggaatcaa
     2461 tactgcctac aggaatggta acaatctttg gctgatgctg agtatatttg gcaaattgct
     2521 cttgtagaac ttcattttgt ctatcagtag acacgataaa gaagtcaacc ttatctgcat
     2581 tggtaaactg atagtcataa tagttattcc aaaggatgta gtcctcattt gtagcatttt
     2641 cactataatg ctccgcatga acaactaccg ctaaatgtgc tgtctgtgct tcctcaaaca
     2701 caacctgacc aatgcctgtc tccctatcga gaatgaccaa atcagactta ttcaaattca
     2761 aagatttcat aaaggcacgc acaaaagctt gctttccata gaaaatctta tccttgaaat
     2821 gataaacttc ttccttccct tgattcatca agatatcata gactggagtc ccgtcttcat
     2881 tataaaaagt tcgttggtat aagactgcaa cattgtcctt gggagcaaaa tactcgctac
     2941 aataacgcgt ataagaaaag taatccttcc gaatcaggtt tcccttaaaa acatactcgg
     3001 catgttgaac caagtccttg ttctcatcaa ccaaataaca ggttacaaac ttatcttggt
     3061 caaaaaagaa tacacgtaaa accttgccat ttttttctct gtgactttct tcaccaccaa
     3121 agtaagccaa gacatcatcc actgtcacgc tagtaggtgc aattttgata tctgtgaaat
     3181 gattataaag ccagataacc tgattatcat caaaaccaat attggctgtt aagtgctgaa
     3241 tattatcggc taaaatcata tctgtaaaga taaacttaga ggacagattt aatttccgaa
     3301 aaacaccagc acgataggct tgagcgtatt caacaccgct actagcccaa ccaattccta
     3361 aatttatatt gtaaattgtc atattttctc ctcttatgga aaatgagtca cgatttcacc
     3421 ttttggagtg acctcaacca gccccatcag gagattacgc accatatccg cacggatttg
     3481 ttctttcata gcttcaaagc ccgcgtaggc ttcttgatag tactctacga ttggattttt
     3541 ctgactagca gattgaccac cgatagccat ggatagctgt tgtagatagt ctacctgctc
     3601 tacccagttg tcatcaatgg ctttaagcag tgaaagtcgt aaaaactgtt catataagtc
     3661 atgttgatta agtaattctt tcttttcaga aagttcttta tcaatcacct gcttcataaa
     3721 gctacgaact gcagttttgt cagttacatc tatataatct ggaacctctt taacatgaaa
     3781 actaatattg gtcacaataa agtgaaacaa taattcacga ctagcatagt gatcagccgc
     3841 tacctcttct gtatatctct caatgatatc cacaacaaca tcctctaagt cacgagaacc
     3901 atctattaga cgattacgct ccttgtaaat catatcccgt tgaatattca tactttcagc
     3961 atattctaga gtctgacgac gtgccgaacg gccagcacta tcactagcat gttgagcctt
     4021 ttcgactagt ttccggtatt tacgaccttt caatacttcc ggttgagtca tatcttgaac
     4081 ctgatagtct ttgtactttt tatgcaccca agatggacca aatttcttga taacatcatc
     4141 ctctaaggat acaaaaaatt tactcatacc aggatctccc tgacgaccag aacggccacg
     4201 aatttgtagg tcgatccgct gactttccat ccgctcagtc ccaataacaa tcaagccccc
     4261 aagctctgcg actcctttac caagcttgat atccgtacca cgtcctgcca tagaggtagc
     4321 cactgtcaca gcccccatct gacctgactc ggagataatc tgagcctcac gcgccgcatt
     4381 attagcattt aggacattat gggcaatccc ttcacgaaac aagagagacg aataaagttg
     4441 agacatttca actgagccta caaaaacgag taaaggattt cccttagcat ggtattcctt
     4501 gatgtactcc aaggatgcat acactttttc aggtaaagtg atatatagat tatctggata
     4561 gtcaatccgt tgtctcggac gattggttgg aatgcgtact acagacatat tgtaagtttc
     4621 aataaactct ttttccgcga ccttacctgt ccctgtcata ccagatatct tattaaacat
     4681 cttaaaaaga ctctgatagg tgatcgaggc catagcccgc gtctcaggag ataatttgac
     4741 atgttccttg gcttcaatag cctgatggag acctccttga agtttagtca tttccattag
     4801 acgccctgtt cccttatcaa ccagtaccat ctcatttcca cgaatgatat agtccttatc
     4861 tttagtaaag agcttatgag ctcgaatcgc ataaaccaaa tgacgagcaa aagacgcatg
     4921 ctcttccttg tataaattat caatccctag gaaattctca gcagacttgg cccccttagt
     4981 agtgagccaa acctcctctt tctcctcttt aaagatataa tcctctcctt cgaccaaggt
     5041 tgttacaagt gtatcaatga tcgcatagta attagactga acacgaggag aacccgcaat
     5101 aatcagagga gtttgtgcac tatcaagcaa gatatcatca atttcatcaa taatcacata
     5161 gttaaacggt cgtaaaaact taccttcttc attcgaggct aggttatcat ttagataatc
     5221 aaaacctaaa ttactattgg ttgtgtagat gatatccgaa gcatagataa gcttcttttc
     5281 ttcagctttc atttccttct ttggaccttc cgtaaatggt acaccaatgg tcaatcctag
     5341 aaaacgataa acttggccca tttcctcggc atcacgcttt gataaatact cattaggagt
     5401 cacaaccatc actccttctc ctgaaaaagc gttcaaatag acaggcatgg tagctgtcaa
     5461 ggtcttacct tcccccgtat tcatctcagc aacatttcca tagtgcatga caatagctcc
     5521 catgacttga acatcatagg ggaacatccc taaaatccgc ttatctgctt cacgcaccac
     5581 agcaaaagct tcaaccaaaa tatcgtctag actttctccc tcggaaagac gctgacgaaa
     5641 ctctactgtt ttagctacta attcttgatc cgaaagagag gacatcttgc ctttcagggc
     5701 attaatctgc tttaaaatct ttttcacttt cctaagctgg aaatcttgac ctaaacgtct
     5761 aaacacttct gatctccttg atagaaaaat gataaaaagt taaggactca aatccagcac
     5821 ttaaaagaga aacttgataa gtataactat cttctggata agtaaatatg aaatctttcg
     5881 ctttttctac ctttttttct aaaatatcac catagcggtc aaaaaaagtt atcttggtat
     5941 aaactccatt ttcaggctga caatcaaaaa ccagtgatag ttgatactcc tgctttcttt
     6001 ttaataaggg aagctgaggt tgggttcgtg cagcctggta attgacacta gaaaaccaag
     6061 tctttagaat ctcaccagat ggcaatagag gattatacag acgaacactt ttgtcagggt
     6121 aataagatac agttgatcca tacatataag tcccaccaac ttctccccaa tgaatagact
     6181 gtctctgtgt aataatcatc ttttctccct tccaaaacca gtctctaaga ccattctata
     6241 aaagtgcaaa aaccaagaaa tattggtatc tgtatcatca ttgtgtcgtc cagcagttcc
     6301 tttagataaa atcttcacac ctgtattaca cagataagac actaattgtt cataggcttg
     6361 tggatccatt tcttcatctt tcatatagga taaaccaaag gttgtctttg aaaaatttgc
     6421 ttgtttaaaa attttccaaa aacgctgatc caactccgtc atatcttgag aacttgtccc
     6481 acctgtatga tgaattaaac aatcaaaagc taagttagag acacccggtg cgtccaaacg
     6541 tccacgacta gctatagttc ctaaattagc caagggctta ccaactatga tggcatgggg
     6601 ttcaaaagaa gcaccataat agagagccgg aaacgttccc atagaaagac ctgataaaat
     6661 caaatcctta tgatcaaagc ctaaataatc aagatagtga gtgatcgtat cctttacttt
     6721 tccctctagc tcatccgttc cgagataaaa agctcccccc tctaaacgtg gatcagaaaa
     6781 taaaatgaag ggacatccaa gagttttcat cataaagtaa ccctcgaatc cttctgcagg
     6841 tcgataacct gcaaaataga cagtcaaagg cggtttaaaa tcacctggat ggaagaaata
     6901 gtttatttca tcacgcttgg aatcatgtag gatatttcca ccaagtacaa acttaccaaa
     6961 ttgttttcga ctccatcgtt ggtgaagatt acctagtttg atagttccac gacctcttgc
     7021 ttcaacagat atcgagagaa agtagtctcc gtctcgacta tccataaaaa gtgcttgctc
     7081 caagtctttt tcagatacta ttacttcttc aaagaaatca tcaacagacc cactccacat
     7141 tttacggatt actaaacgaa agtcacaatt gccttccttc tcatattcaa gccacaactc
     7201 aatagggagt gttttttgca ccataatatt ataagcccaa taggcaagct gggcaaaatc
     7261 ttgcccaaac tcaccttcca aactgacata atccaatcct tgataagaaa tagctcctgt
     7321 aaaatttgga ttgacttgta tcgtcggagg aaagagttta tcaccatacc caccgccaaa
     7381 aagagattta cttaaatcat tgattagttg ttggggactt aaaaaatcaa ttgcttgtgc
     7441 acatcgtttt tttagaaaat ccacaacctc tctatcattt atttgtagat tctggttata
     7501 gaacaaagta taaggttcaa aatattttac ataggaaaat aagtctctgg aatactgacc
     7561 atcttctatt aaaactgcat gaaaacggtt aatatcctct ttttcaatta tttcacgaag
     7621 ggtttttgat gagtttgggt aaaaataata ccaactcata ttttcaggta tgtcatagtg
     7681 attttcccaa ttggcaagtc ctatctgtaa aatattaagt tcctttgaca tttttaacct
     7741 cctctatcca tttctctaat tttccaagaa attgttgtcc tgtgtgctcc ttaatcttat
     7801 caattgaata tatcaaggac tcattccact ccttcaacct atctgtgtaa taatgtgcag
     7861 ccttagaaaa ttctgtaaca tctgctaaca aataaccatt ttttaaatgt tcaacataga
     7921 cggtttcaac taggttgatt tgaggaattc ctgcactaat cccagctatc tgtgtgtaga
     7981 gatgaggatg tctatttaaa tccacaatca aacggacaaa ttctagtgtt tttatcaaat
     8041 ctaactcatc attcaagttg acaaactgta tacgtaagtc ctggtgttga ttttcttcca
     8101 gaggattttc tgcatcacca taatcaatcg cctttcccag attttcggat tgaatgtttt
     8161 cttggatgaa agactcaaca atcccttcaa cctcattcat ttgctcctga ctagcagcaa
     8221 aggctccaaa aatcacctca gtatccttat tttcagcgac aaaggacaag acttgaagca
     8281 gagcttgatt atcaatcccc tgctcaaaat ccagttgaaa ataaatgatg gattctttct
     8341 tagtttggct tcgtcccaat cgtaatcggg tgtcaaaaga tgataaatga taacattgat
     8401 gcattcgttc aggatacaac tcctgcaaca atcgtaaact atcctctcta tccaccaaaa
     8461 tcaaatccga tttttcaaaa gttacatcta aatccctaaa ggtatcttga ggattacgtc
     8521 caatgaacag actcaacagt ttaggattag tactaggtaa acgatctagt accaactggt
     8581 catgatgaga atgagaagga agcataaaaa tatcctgatc cttcacatac gtttgcagat
     8641 aattctcaaa aaattctgcc accagatctc ccatattttg ataggtaagc attttaaagc
     8701 gataacccaa aattggattg acttctacct gtcctccttc ttttaaacgc tctctaaatt
     8761 gccaaactcc cttaggattc aaatattctt gataggctgc ttgcccatct tcaaaaaaaa
     8821 taatactcga tataaaccct cgatcatcca tgatatggtg catatgtatt tgaccattcg
     8881 gtccaaaata ctgtatatca ctgatgaagc cttcaactcc atgttcaacc ttagcaaatt
     8941 tcttcccatt tttttgaacg ataatcgtaa agggactata aataaattca cagtcatcat
     9001 cccactcaat atctctaatg cttaaaactt gggtatggag attatgaaaa tcttgcataa
     9061 catcaaaaac ggaataagta tccgtttcta acacaccatg tctatataaa aaataacgta
     9121 gatgaggctg gtaagctaat actaatagac gagaatctat atcttgctct tgaaagagcc
     9181 gaatctggtg aaaggtatca tcaaactcta gacgaaaatg agaaaaatac catggagtga
     9241 tatctgcatg ccatgttctt tctgacccat accaagctgg aataaaataa tacataaaag
     9301 tctcctaaat caaaggttta taggtctcat ttagccttaa agccttgacc tcgtctctaa
     9361 tcgtaaaaat catgcctcca aacatcataa acaaacctgg aatcattgcc aatcgtaata
     9421 acttttcatc aaacaaaaca aaaagcatgg gaccacctgc cataaccaca ttaaagagac
     9481 cacctatgac tgagaaacga aggaccaatc gattaataaa tagactagta tccgcacctg
     9541 gataaatacc ataaatgtat tctccagatt ttttcatacg gtctgcaatc tcttctccat
     9601 tcatcgtaac aaaagcaaaa atgatactaa ataaaaataa gaccgaaata taaacataga
     9661 cccacaaagg ctttccaatc ataaattcct ttgataaagc cgctaaccct gaatgattag
     9721 ggaaaataaa tcccaacaag atgaacaaat aagctggtac actaagaaaa ctcatcacat
     9781 acatataagg catcccacct gcaggattca acatgatttc gagataagaa tagcgtttaa
     9841 atcgattgtg taagccaatt ttattaacag gaaccaaata gcgagctcga tacataaggg
     9901 caagtaaata agaaaagaca agggttaata aagcaagtaa cacaataatc cctgttggaa
     9961 tgtgtactgt ctgaaatgtt tccaaaacat cctgaggaat atttaaaacc atactggata
    10021 ggaggattac aatagaacct ccaatcccca tactcgcatt taaatctgac aaccaaacaa
    10081 gaaaaaatgt tcctgctatc agcaacaagg tattcattag aacaaccaaa attaccgagt
    10141 aggaggattg tactggcaag ctcaagctaa ctgccaagga ttgaatcaca gcaattagca
    10201 aggtcagata catttttcga cgatcttgaa tttcaatcgc tgtagaactt agccccaatt
    10261 ttttagaata tgaaaacatc tgccacaaaa tcatagctga catccaaggg gataatccaa
    10321 cagaaaaaat tgacaaactt cttagatttc cacctgttag ggcggctgag aaggctagat
    10381 aggctgttga acctcctaaa aaatctttag tatttaggtc aacaaaaggg agaataatac
    10441 gacttcccaa tacatagata aacagtagaa ataaggtaaa tagtcctttt tttactgcta
    10501 ttgacgaata aatcttagtc ataaaacact cccctcacct tcattgtttg tgatatggta
    10561 aataacaatt cttttatttc aacttttacc atttctttgt tctgcatcaa aaaacaactt
    10621 taatctgaaa caaatatctt tatattcctc agtatctgtt aattgatggt attctaaagt
    10681 ttgcttataa tcttttaaaa gattgacaaa tcgaatacgc agaacggaga tatccaaacc
    10741 agccaagaca caatccgata ttttcttaga aaacacttca acaatatatc ttgcccagga
    10801 aagatcaaat tccgttctca tgatgcttcc tgaacgtctt ctatacagat agagattatc
    10861 gtttataaag acgattttag aggctaaaag ataaaagcga tgcatagttg cttcatcatc
    10921 aaagcggcga ccatttgaaa agtgaacttc attgaataat tcttttttaa tcaacttaaa
    10981 tgtcggcaat ataaaggccg agctattgaa tttccaatct cctgcttgac ggttcataat
    11041 ttcttgtata gctaattctt cgacgcaaaa atcttgccct gttacataaa aataaaaatt
    11101 cccgtcactt tcgtcataat tatagtaatt ccctataaca acatctgcat ccttttcttg
    11161 gacttgctga taaagagttt caatcatatt ttcttttacg atatcatctg aatcaacaat
    11221 cgtgatgtac tctcccttag ctacactaat ccccatattt ttagcttgtg ctgccccccc
    11281 ttattttctt gatgcaatac tcgcacacga ttatcttgca tcataaaagc atcacaaatt
    11341 tccccagaat tatccgtaga agcgtcatca actaaaataa tttctatatt ttgataggtt
    11401 tgcttcagaa tatgctcgac acactccact aaatattttc ccgtattgta tatcggtacc
    11461 acaacactaa ttaattcact cattactcta tctctatatt cttcattcta tccactaaat
    11521 catctggtct ctccacagaa aaaataccgt catagagtcc atcatccata cttttacgtg
    11581 tgatatcaaa agcgaagatt ttcttgccac tttcttgtgc ccttgtaact acattaaaaa
    11641 cctctccacc tgtattaata tctaaataaa ctatagaatc gtccaatagc caatcaattc
    11701 tactatgtaa tacattttga tatactgtta cattcgtata ctgtgataga gaggtcagct
    11761 cctcagaaca atcacacggt gcagccaaat gaaaatgcca atcaggtaac cgttgtacta
    11821 aatactctaa atgttttata tctgcactcc atgtcacaag catcacttgt tttttagact
    11881 gattgcttct ttcaaaataa tttaaatcgg aacgttgaaa agcaatctct gaccaatcca
    11941 aatctctata aacccaccat aattcacgta gtctagatat actatatgta ttccaaggtt
    12001 tatcatgact agcatagtga acaattgtag gtggattgtc atctagacgt tcacattctt
    12061 gagcaaggtg ataaatatca acacccacca tataattata tgttttatct aaggctagcc
    12121 aattatcctc aaaataaata tttaaaatac tctgatcccc aagatgaaca acttgattct
    12181 gctcggccgc taattccaat aaactattga caatagaatg ttctttccac tttgcaacat
    12241 ccattagtaa cataccagca ttaaatccag attttcgtcc ttcataggca tagacatcat
    12301 caacagcacc aattgaatat cctttgagat ctatctcaaa caaagtagct agttccccag
    12361 ttacaatgat atcggaatcc agatacaata ccctatcaga ttcaaccact tctgtcgcaa
    12421 aaaatctaaa gtaagaagca taatttatat gaggtcctgt tttatagctt tcaaaaagtt
    12481 ctttatcaat gtgtacactg cgaattgtag aatgcacaac ttccattttt ctccccaata
    12541 atttaaacca ttcaggagct atatcactgt tgagaatata aaaatcaaca tctctattgt
    12601 gatacaatac agactttata gtcgtctcta aaggaataag ataggcatta tctgccgcta
    12661 atactattga ttttctcata ctgttctcta cctcatgtaa gatctgattg ctgcaaccat
    12721 ttttttcgga atggagcttg gatagatacc ttcatatgaa atctcagaaa gattttcaga
    12781 gcaagtattg tcgaaagcta tcattggttt tttgtactta agcacaaact catatgcatc
    12841 ttctaatttt cgatcatgat tcaaatccaa atacaaatct gaagctatta ctatttgttc
    12901 caataacatt ggtaaggaat ttggatatac ggtaacatta ggaaaacgcg ttaaagctag
    12961 tagttcattt gccatatttg tataagcaac aatatgaaaa tgaacttctg gcaattgctc
    13021 cgccaaataa tcgattttct cgacacacca agaattcgtt aaattaacac attgtaactt
    13081 aaatatttgt gatttactag gatacttcac actctttgaa aaccattcat ttaaaataac
    13141 agaccaatcc atcaaagagt attcccacca aacttctctt agacgtccaa cagaaaattg
    13201 attccaaggc ttatcctgag aaatatagtg taaaattagt ggcagtggtt cgagcggaat
    13261 atcaaaaatg aattgatgtt taaaggctgc cgccccataa tcatatccta tttgaaaatt
    13321 atattgatct tcaagggaac tatattgatc tttaaacaac atattcaaaa ttgactggtc
    13381 tccttcttcc acattctcat gttctttttc tgttaagtca atcaattttt gtcgaatagt
    13441 ttcagatccc cattttttgt tgttaatcaa gagaacacca gcattgaagc cgactcctgc
    13501 tccaaagcaa gaacgagctg ctgccaaata attttcacct aagtctaatt caaacaaatc
    13561 ggtcaaatca ccagtcacaa tcaaatcact atctagatag agaactttat cttctgttac
    13621 aaaatctgga ataaaatagc gtgcaaatgt catatgattg atatgaggta atttattaga
    13681 ccaattcatt tgaaactgtg agccaattaa cttgcagtca atcaagtcgc cccccatctc
    13741 ttgtaaatat attcttattt gactaaacca ttcctgagga atgtcctgat ttagcagata
    13801 aattttcaaa tgactattgt gtctacagag tgacttcatc gccgtttcga tttgtcgaat
    13861 ataagcgtaa tcacctgcaa atactacagc gcgtttggta tttctcattt taaatcctta
    13921 tcatttattt taaatagtct agtttaatat tcgtgcaaca atctgactgt tataggtttc
    13981 atcatcaata aattcaatat cttggtcgaa attatgttct gcagtatgtt tatgagaaaa
    14041 tttaacctta gttttagctc ccattttcat ccattcatac tctgagtcaa catggcctac
    14101 atccaaaact tgatagccca tctggcatag attataactc agaacttttg ctgtaggacc
    14161 cagcatacat aaaatcagtc ttccttcagc atgtttcata atttcctctt gaatgttatc
    14221 gacaacggaa taagcattat gagatggaca aataatcctt ttcacagatt tgaccttatc
    14281 aaataaatca tttccaaccc cagagcgaga tgtaatcccc tctacaatca gaatatccct
    14341 attctcccaa atactcttta gcttttcaaa ttgtcctttt gccttactct tatcttcata
    14401 atcaatgtat ggacgcgaga taaaggtaga gccataccat gaacccttac acaactcttt
    14461 ataaaaatcc atataaacat tcatatgttc tttccagaat ggtgtagccc accaagtaaa
    14521 gacaaaacga tcgtcaaaag catcaggtaa acaaaccact aatttctcgt cactctcaag
    14581 acccacaata tgttttaatt ggttagctaa ctctagatca tgattttgat aaggtatagg
    14641 caaaccccac atcaaattga cttctccgtc tccaaaacga actaatgagg acttattttt
    14701 gataacataa tccagagtct gatcaattcc gataaccttg attggcttct ctggttctcc
    14761 caaagtctct ccttttatca atgcatgaaa tgcttgaaga agcctttctg gatgctcact
    14821 tgaatataaa tgttgaccat gatttccatt ttgtgaagta taaaatgcca gcattggttt
    14881 attcaatgat gatatctcct tgacaacctc actagtagaa ttgtgtaggt taatatccaa
    14941 ataaacatcc atttgagatt tgagtttttc aaattgatcg attttcactt caggatatag
    15001 cttaacattt ttatattttt ttgctagttc ttgtaacatt ggtcccataa ctgtccaagc
    15061 agcaacttga aaaacaacat ttggcaactg tccagccaag taatccaatt gttcgatatc
    15121 ctgagaagcc gtaaacgtta aacaagttaa acttggaagt tgtcgaaaac aggtaagatg
    15181 tccgtttttc ttaaaatctt taatcgtatc aatcataaag gaaactttcc cattttcgaa
    15241 aacgatttgt cctaactctc cgtgctgagt cgattgaaaa gctagtagcg ttttttcttg
    15301 ttcttgcaaa gattttaaaa agttctcatc tgcactacca tgattgatat ccaaatatag
    15361 atttgttgac tttttcagct tatctaatac aggaggaaca atttgtggat gcaatctcac
    15421 attattatat acagctaatt tttttaattt atctcccata tccgtccaag ctgcgatatg
    15481 aaataccacc tcaggtagag ctgtaactag ctcttctatt ccttctaaat cttgggaatt
    15541 cgttaaggtt aagcaagaaa attccttatc tagaggcgat attagttcaa attctcccat
    15601 atggtgttgt aaaatctgac tccactccaa atcatggaat tcccaccata aatcacgata
    15661 acgattggct gtcaaggtag tccagggttt tctgtaggtc gtaaaatgaa tcaccactgg
    15721 tttatcatta aaagccagat gttcctgcca attgttatac aaagccacaa tatcatgccc
    15781 tacttgtaaa ttataagctc gacctagctc taaccaatca tcttgtaaga cctgattaaa
    15841 aatcgtttga tcaccattaa aatgctcgaa acggccttct tcaacttcct tcattgtaac
    15901 aatgctctgt tcaattagtc gttcttttaa tttctcttga cgccattttt tattgttgat
    15961 taataaaaca cctgtattaa atgtaattcc atctgtatct ttaactgctg cgagtgattt
    16021 ttcttctaaa taaatactaa ataatttctc taaagaagta tttacaatca aatcactgtc
    16081 taaatataaa accttatctt cttggatata atctgcaata aaatatctag cataggtaat
    16141 gctactaatg tgatcttgct tttcccaatc ttgaaacaca gtttgttcag gtagcttcac
    16201 atcgataatc tcactaccta acatgcgagc tatttttcgt ggctttcgaa accaatctgg
    16261 catgatatct tgatttaaaa tataaatctt aacatctcga ttgtggtata gaatagattt
    16321 tatggttgtt tctaactgcc tggtgtaatt acgatcacca gctaatacaa ttgttttgtt
    16381 catttcactc ccttaatcaa ttcccaagtg ataaatcgca tctaccaata gctttttagt
    16441 gaaatagccc tcttttaaca aatagctaaa ggtcttgata cgattcgtca tttcttgata
    16501 ttcttgtaga ttcattttat caactatcgc atgtacttcc tcaaggctat ccgccataaa
    16561 acctaagccc tgatctacta taaatttagc agttgataag ttgctcggta caatgactgg
    16621 gatgcccgct gttagatagg tactaacctt atgagatata tttaaagtat agtattgatt
    16681 gctttctccg tcattttgat gcgttcccca gacaagacca aagccacctt ttgataattc
    16741 tagcaacagt tcctcatctt ttttccatcc ttcaatgcta agatttctag cattggaact
    16801 agcttctcct ttatttgaaa acaccctcaa aggcgtatct tgagaccaat tttgtaagtc
    16861 tggaaaacgc tctaaacttc cagcaaaaaa aagttctttt ttaaaagcag gggtgtataa
    16921 ggataaatca tgaggatgat cccacatccc ttgaacaaga atcttcttag tcgtcaatcc
    16981 ttcttccatc aggcgtgttt tcattctctc tgacggcact atcaaaacat ctgatagatt
    17041 atacatgtac atataatctt tcatgagata atagttacta tcaaacatga ggggaacaac
    17101 atcatggata aagcaaataa ttttcacctg catatccttt agcttatcaa acaagagacg
    17161 atcaaattca aagccattcc aggttggaga ctgaaaaact aggatatctc caatagagat
    17221 actggccata atgccatcca gacgcttatt catttcagaa ggactatctg aagaaatatt
    17281 gtaaaaatag atgccaacct ctctaaatcc cagttgactg gctatctttt gaacagcatt
    17341 ttgcgctaag ataaccgtac tatcaccagc catgccgtat aaatttgtta aatgtagttt
    17401 catattatgc tatctcattt tttattacta tctacggaag taaagacaat ttttccatca
    17461 ttcttctata aatttctgtg aactgcatat tttgttcttc taattttgct actactcttt
    17521 gtaatctatt tttataaatc aaaatatgat tggtcaagtc atagtctgaa cttgctaaaa
    17581 tagctatctt ttcttcccta gccccaattt catcatacat acgcttttca ctccatgtat
    17641 tcgataaagt atcagataaa ccaacacggt accagtaaac acaacgattc aaatagacta
    17701 tcctcgaagc tcttagaagt accttccatg tccagtaagt atcctctgca atttttccta
    17761 taggaaatgg taaatcttgt agtaactctc tcttgaataa cttcaagaca gctacagtcc
    17821 agttcccatt tctgacttct tcgacacctt ccctatccat gattgcttta ccttctatca
    17881 cttccagaga atcatctgga tccgtaacat aagtcatata cacatagcgt gtttcatcat
    17941 aagaattata acgcccgata ctaatatctg cgttttcctt tttcaaagca ccatataatc
    18001 ggtctaaagc atcatgttcc aaccaatcat cagagtctac aaaagtaatg tacgcccccc
    18061 ccgaacattc aatacctagg ttacgagctg atgaaagacc gccgtttgct ttctcaaaat
    18121 atttgaaacg agaatctttc tctacaaatt cttcacatat tttggatgaa tgatctggag
    18181 agccatcatt gattaataaa cactcaaaat tttgatacgt ctgattctga atgctatcca
    18241 aacacattcg caaataattc tcaacgttgt agattgggac tacaatacta attagttcat
    18301 tttccatata ctaactttcc tagacttgat ttattctatt tccctcttaa tctcttctct
    18361 ttacaaaaag atttccatta ttataaacta cactgtttga tggaatatca ttgcgtatga
    18421 gacaacctgc tccaataatg gtattatccc caatcgtcac tccttttaaa atcgtgacat
    18481 tacttcctat ccagcagtct ctgccaactc taatcggcgc tgtagtccac tgccactttt
    18541 caatcttttc agcagtataa atatggtcgt gatcataaaa acgaacaccc tctcccatca
    18601 tggtaccagc tccgatttca attctttcca tacagttaaa actacaagag ttgttgataa
    18661 aaacaccatc attcaggatt aacttaccag aagagacatg aaaattttca aaatttctgc
    18721 agatgacatt tgctcctaca atgaagtgag ccttatcatc gattttaaag acattccaag
    18781 tcccatcttg gatgattttc tcttgatgat tggtgcctac tctgacttca ttcagatagt
    18841 ctctcactct agaaaacaat ttgtctggtg tacttgatag aaaaacttcc tgcccctgat
    18901 ttccatggac tgtattgtca aaagcaaaga cagattttcc agcctgtaca aacttggaga
    18961 caatagcatc tacttcttga aagtgattga tatctagtaa aaagtcacag gtatcgacta
    19021 gaaagtttag ctgttcttca tttgtaatcg ttcggagacg gacattatag ttcgtttcta
    19081 agctacggat tttctcggaa aattgaacag gtgctgcaat ataaaaacag atatttggca
    19141 aggattcaag gagataatcc aactcttcaa tctgatcact aacagtcaaa atcagagcat
    19201 ctcctactaa atgatttttc ttcatttgct tatttctctc aatttctcaa tcattgcttg
    19261 aacttggtca acagcatata cctcctgacc tgcttcatag gttttggtat tttcaaagga
    19321 caaaatagtt tttccaagtc ttgcaaattg attgataatt tcctctgttt tttcaccatg
    19381 attaatatct aaaagtactt gactggtttc taccaattca ttatcgacat ctaccaaata
    19441 gtgaattccg ttaaatatag tcacgtttgg ataaattgtc atctgagcca atcgatcact
    19501 aactattact ctagctgcta tcttaaactg aatatcaggc aaggattgaa ccaatgtctc
    19561 aatttgttca atatggtctg aggcagtata gattagacaa gtgaaaggtt cctttattgg
    19621 atagatgtga gatctttgta atggatgtaa atgatggttt tgtcccaatt ctgtccattc
    19681 aagcccatga tagtaccacc aaacttcacg ataggtttgg gccgccaaat ctttccacgg
    19741 tttccgatga gaaagatagt gaataatagc aggataatcc tgaccctcag gcaattgata
    19801 atcagcaaac tgtttatgaa tgacaatatg attataatca aagtccaatt ccaaccattt
    19861 atgttcaaaa agcatattca agatgctctg atctgcctga tccaccttat catgccattc
    19921 attggttaaa tcaattaatt tttgggtcat attctctttt ttccaaaaag cattgtttac
    19981 caagagaaca ccggcattaa agatttcttg accaaaataa gctctgcccc caaaatctct
    20041 aacagcagcc aaaggataat cttgtaagtc tgtagcaaac aagtcatcca gatttttcgt
    20101 tacaactaga tcacagtcca agtagagggc cttgtcttct tgcacgaaat cagctatgaa
    20161 atagcgtaaa aagactgtgt aactaatatc cgatttataa catgaaattt gctcagaagt
    20221 tacccgacaa ttaataattt ctgagtcaca cttctctaag cgcttattta attgcttaat
    20281 ccattcattt ggaaaatcgc tatgaatcag ataaaaacga atcgaacgat tatgataaca
    20341 aatagacttg attgtcgtta aaacttggtc tacataggca taattcgccg ctaggacaat
    20401 ggctttcttt tcacttgatt cttgtaccgt taactgattt aaaagtcttt ttttcatttc
    20461 aaactcttta tacattgctg tgtcagataa accgctagct tgaccgttgg cgagactgac
    20521 ttccaacatc tgacgataaa ctgccaagtg tttctctaga ggataaccca tattagctag
    20581 tagcgtaata cgttcagaca tagcatcaac taaggcgtgc atccactttt ctgtccaaac
    20641 tcttgataaa ctaccttttc taatccgata agcataaaga cctttattta aataaattac
    20701 cttttccgat aataaatata ccttttgatt gaggtaacca tcttctccta atttacccat
    20761 gtcaaagcgc aactgctcaa acaatcttgc cttatagagt ttaccacaag cagatatcaa
    20821 agcaaaactc ttcatttctt gagtttcata caagttctca aagatggaaa cattgtcata
    20881 taccttctca taataggagt ctcctgatat atgaaagtag aacattcctt cactttcgtt
    20941 gaaagaatag taattcccaa ctgcaatatc agcttgatat tctgttattt ttttatatag
    21001 ggtttctaca taatctaact caatccaatc atctgaatcc acaaaggtca cataatttcc
    21061 ggacatatta ttcagaccgg tgtttcgtgc agctgacagc ccagcatttt cttgttctat
    21121 ataggtaatt cggtgatcca tttctgcaaa ttctttacaa atttcacctg aagcatccgt
    21181 agaaccatca ttaacgacaa caatctcaat atttttatat gtttgagtaa taatactatc
    21241 tagacatttc ctcagatagt tttctacatt gtatactggt acaatgaccg taattttatc
    21301 atccactagc aattttctcc ttgatttatg tgcgcgagtt agtttagcct aactttacag
    21361 gttgtcttac tcttcttcat cacgtttacg gcgtttcgca accaatccaa tacctgtaac
    21421 agctgctaga actccaagta acacagatcc aattgacgac tcagtacctg tattaggcaa
    21481 ttctttttgg gatttacctg tcgatccaga agcatttcca acttgtgagt tcgaatggtt
    21541 tgctgaattt gaaactgatg tacttgctga agctgacgca ctcgttgatg ctgaggctga
    21601 ggcgctagta cttgctgaag ccga
//
LOCUS       DAVMHG010000021        21564 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMHG010000021 DAVMHG010000000
VERSION     DAVMHG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.021"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            187..510
                     /gene="tnpB"
                     /locus_tag="VYR08_000625"
     CDS             187..510
                     /gene="tnpB"
                     /locus_tag="VYR08_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3466196.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(651..938)
                     /locus_tag="VYR08_000626"
                     /pseudo
     CDS             complement(651..938)
                     /locus_tag="VYR08_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            929..1327
                     /locus_tag="VYR08_000627"
     CDS             929..1327
                     /locus_tag="VYR08_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3466197.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(1524..2444)
                     /gene="cysK"
                     /locus_tag="VYR08_000628"
     CDS             complement(1524..2444)
                     /gene="cysK"
                     /locus_tag="VYR08_000628"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV3466198.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(2729..3364)
                     /locus_tag="VYR08_000629"
     CDS             complement(2729..3364)
                     /locus_tag="VYR08_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV3466199.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            3420..4718
                     /locus_tag="VYR08_000630"
     CDS             3420..4718
                     /locus_tag="VYR08_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3466200.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            4715..5377
                     /locus_tag="VYR08_000631"
     CDS             4715..5377
                     /locus_tag="VYR08_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV3466201.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            5457..6005
                     /gene="raiA"
                     /locus_tag="VYR08_000632"
     CDS             5457..6005
                     /gene="raiA"
                     /locus_tag="VYR08_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV3466202.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            6141..8114
                     /locus_tag="VYR08_000633"
     CDS             6141..8114
                     /locus_tag="VYR08_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV3466203.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            8111..8563
                     /gene="rplI"
                     /locus_tag="VYR08_000634"
     CDS             8111..8563
                     /gene="rplI"
                     /locus_tag="VYR08_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV3466204.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            8607..9959
                     /gene="dnaB"
                     /locus_tag="VYR08_000635"
     CDS             8607..9959
                     /gene="dnaB"
                     /locus_tag="VYR08_000635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV3466205.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            9961..10227
                     /locus_tag="VYR08_000636"
     CDS             9961..10227
                     /locus_tag="VYR08_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466206.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            10316..11662
                     /gene="cbpD"
                     /locus_tag="VYR08_000637"
     CDS             10316..11662
                     /gene="cbpD"
                     /locus_tag="VYR08_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV3466207.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      11745..11847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11918..12208
                     /locus_tag="VYR08_000638"
     CDS             11918..12208
                     /locus_tag="VYR08_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV3466208.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            12171..12929
                     /locus_tag="VYR08_000639"
     CDS             12171..12929
                     /locus_tag="VYR08_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466209.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            12968..13975
                     /locus_tag="VYR08_000640"
     CDS             12968..13975
                     /locus_tag="VYR08_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3466210.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            13975..14703
                     /locus_tag="VYR08_000641"
     CDS             13975..14703
                     /locus_tag="VYR08_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466211.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            14822..15280
                     /locus_tag="VYR08_000642"
     CDS             14822..15280
                     /locus_tag="VYR08_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV3466212.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRASMLRQIIQEVDRKGK"
     gene            15282..17714
                     /locus_tag="VYR08_000643"
     CDS             15282..17714
                     /locus_tag="VYR08_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3466213.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            18356..19009
                     /gene="rr06"
                     /locus_tag="VYR08_000644"
     CDS             18356..19009
                     /gene="rr06"
                     /locus_tag="VYR08_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV3466214.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            19006..20346
                     /gene="hk06"
                     /locus_tag="VYR08_000645"
     CDS             19006..20346
                     /gene="hk06"
                     /locus_tag="VYR08_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV3466215.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            20429..20956
                     /locus_tag="VYR08_000646"
     CDS             20429..20956
                     /locus_tag="VYR08_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV3466216.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            21035..>21564
                     /locus_tag="VYR08_000647"
     CDS             21035..>21564
                     /locus_tag="VYR08_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV3466217.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKVAEAKKKAEDQKE
                     EDRRNYPTNTYKTLEL"
BASE COUNT         6671 a         3582 c         4838 g         6473 t
ORIGIN      
        1 tgttttttcc ccagtctcac tcgattagaa taattaaaac aaaaagccct ataataaggg
       61 ctaagtgcat ttaggagact atacttcaaa ttcatagtaa gcgcgtcata acaaggtatc
      121 tatcctaatt ccccaacttc cagtatacca gataaaaaat aattatcgga ggaaaggtat
      181 tgtactatga taattcaact aagtgattta ggtcaagttc accttgtttg tggcaagaca
      241 gatatgaggc agggaataga ctcattagcc tatgtagtta aaacccactt tgaattggat
      301 cctttctccg atcaagtctt tctcttttgt ggtggacgta aagaccgctt taaagttctt
      361 tactgggatg gtcaaggatt ttggatatta tataaacgct ttgagaacgg aaaactgact
      421 tggtccagta cagaaaagga tgtcaaagct ctcacatctg aacaagtaga ttggctgatg
      481 aagggctttt ctatcactcc ccaaatatag tggatttaaa ctagaatagt acacctctgc
      541 ttctaaaaca ttgttagaaa tcgatttgac tatcctgttc ttatttcatt ttactataaa
      601 ttcatcagaa agtcgtgatt tctattgaaa tgaggacttt ctttttatac tcatctgttt
      661 tcaaaaagca ttctagtccg tctccgatta acgatggact ttatcacctc cttctccagt
      721 ccttgtatga catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc
      781 tctttccaca cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag
      841 ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg
      901 agtaaaagat aatcatctgg ataagcttgt gaaagctcct attcctaaag cccctttagc
      961 gcataacttt ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa
     1021 ggtacccaat tatcgccaag aagaagattg ggctaggatg ggtttaccaa tcacacgtaa
     1081 ggaaatctct aattggcata tcaagacgag tcaatactat ttggagcccc tttataacct
     1141 cttgcgagag agactattga ctcagccctt acttcatgcg gatgaaactt cttatagggt
     1201 gctagagagt gatagtcagc tgacttacta ttggactttt ttgtctggga aagctgagaa
     1261 acaagggatt acgctttacc atcatgttct aatcgatttg ttcatatctt atttcaatcc
     1321 attataaata gtgagaaata tctatcctat cttctagaat gtcttccaaa cgtggaaact
     1381 ctcgtaaaca aagaggtttt agaggcctat ttaccatgga ctaaagttgt acaagaaaag
     1441 tgcaaataag aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt
     1501 caatagactt cgttattgga cggttacaat tcataaagtg ctgtagagag ataacgttca
     1561 ccgttatctg gtgctagggc aaggactttt ttacctgtac ctaatttttt ggcaacctcg
     1621 atggctccgt agatagctgc agctgaggaa atccctacaa ggaagccttc ttttccacca
     1681 atttcacgtc cgagtgcaag agcgtcatct gatgttacac gaacgatacc atcataggct
     1741 ttagtatcaa gtgtatcagg aataaatcca gctgagatac cttgaatttt gtgaggacca
     1801 ggtttttcac cagatagaat agcagattca tctgcttcta ctgcaaaaac ttgaatgtta
     1861 gaattttctg atttgagtgc atgagaaaca ccagaaatcg ttccaccagt acctactcca
     1921 gcaacaaagg catctaatcc atctttaccg aaagcagcta gtatctcagc tcctgttgtt
     1981 ctttcgtgta cttctggatt agctggattg tcaaattgaa gaggaaggaa accatcacgt
     2041 tcagcagcga tttcttgagc cttagcaata gcacctttca ttccctcgct accaggagtt
     2101 aggacgagtt cagcaccata agcttggata attttacgtc gttctacact catagtttca
     2161 ggcataacga tgacgacttt atacccttta gcagcaccta cccatgaaag tccaataccg
     2221 gtgtttccac ttgttgcttc aacaatagta gaaccaggtt tcagaatacc atcttgttca
     2281 gctttttcaa tcatgctaag ggcaatacgg tcttttacag atgaaccagg attaaatgct
     2341 tcaagcttta tatagacgtc tgcagcacct tctggcacga tgttgttaag tttaacaatc
     2401 ggtgtttgac cgattaattc agtaatgttg ttataaatag acatatgaat acctctttgt
     2461 ataagatatc tttagtataa cgctatccat accatttgta aaatatagaa atcctatcga
     2521 agcgatagga tttctatata tcatagatca aagtcattaa ttttcaagtg attgcgttca
     2581 gctaattcta gtaaataggt gtgaaggaaa acgatatcag gtttcgtttg aattgcttca
     2641 ttttctagta tattttggta cgcatctttt tattttgctc gctaaagtag ttgtgagaat
     2701 gtcaacgaaa cgctgtatta ttcattgttt acactaagtt tacaggaact tcaacctctc
     2761 gtaaaccttg gtcagttaaa gtgacttttc cattaaaaaa ctccacaagt gcagctttaa
     2821 tagtttcttt ttcttcttta tcaacataaa tcatcgtatc gacttgatct gtaaagtttg
     2881 tatccagctc catgagacca tgttctttaa ggaagttact gtactcttgg tactgagcat
     2941 aagacatttg aatagcaatg ccagcctgtt cttttatttc aataatacca atttctttga
     3001 cagctaaggc gacactgccg gcgtaagcac gaattagtcc tccagcgcct agtttaatac
     3061 caccaaagta gcgtgtcacg accacacaga cattggtgag attgtgattt tctagtaccc
     3121 caagcatggg aacaccagca gtaccactag gctcaccatc atcacttgta cgtttaattt
     3181 cactacgttc tccaataatg aaggcagagc agttatgtgt cgctttgtag tgttcttttt
     3241 tgatggcagt aatgaagtca cgagcctctt cttcgctata aacacgcttg gcatggcaga
     3301 taaagcgaga ttttttgatt tcttcttgga cttgaccgtc ctctttaatt gtcctaaatt
     3361 ccatgattta attgtactaa aaaatcatct aaagtgctag tttttacgaa taaagaagta
     3421 tgaaagtaaa tttagattat ctcggtcgtt tatttactga gaatgaatta acagaagaag
     3481 aacgtcagtt ggcggagaaa cttccagcaa tgagaaagga gaaggggaaa cttttctgtc
     3541 aacgctgtaa tagtactatt ctagaagaat ggtatttgcc catcggtgct tactattgtc
     3601 gagagtgctt gctgatgaag cgagtcagaa gtgatcaaac tttatactat tttccgcagg
     3661 aggattttcc aaagcaagat gttctcaaat ggcgcggcca attaactcct tttcaagaga
     3721 aggtgtcaga gggattgctt caagtagtag acaagcaaaa gccaacctta gttcatgcgg
     3781 taacaggagc tggaaagaca gaaatgattt atcaagtagt ggctaaagtg atcaatgcgg
     3841 gtggtgcagt gtgtttggct agtcctcgca tagatgtttg tttggagctg tacaagcgcc
     3901 tgcaacagga tttttcttgc gggatagctt tgctacatgg agaatcggaa ccttattttc
     3961 gaacaccact agttgttgca acaacccatc agttattgaa gttttatcaa gcttttgatt
     4021 tgctgatagt ggatgaagta gatgcttttc cttatgttga taatcccatg ctttaccacg
     4081 ctgtcaagaa tagtgtaaag gagaatggat tgagaatctt tttaacagcg acttcgacca
     4141 atgagttaga taaaaaggtc cgtttaggag aactaaaaag actgaattta ccgagacggt
     4201 ttcatggaaa tccgttgatt attccaaaac caatttggtt atcggatttt aatcgctact
     4261 tagacaagaa tcgtttgtca ccaaagttaa agtcctatat tgagaagcag agaaagacag
     4321 cttatccgtt actcattttt gcttcagaaa ttaagaaagg ggagcagtta gcagaaatct
     4381 tacaggagca atttccaaat gagaaaattg gctttgtatc ttctgtaaca gaggatcgat
     4441 tagagcaagt acaagctttt cgagatggag aactgacaat acttatcagt acgacaatct
     4501 tggagcgcgg agttaccttc ccttgtgtgg atgttttcgt agtagaggcc aatcatcgtt
     4561 tgtttaccaa gtctagtttg attcagattg gtggacgagt tggacgaagc atggatagac
     4621 cgacaggaga tttgcttttc ttccatgatg ggttaaatgc ttcaatcaag aaggcgatta
     4681 aggaaattca gatgatgaat aaggaggctg gtctatgaag tgcttgttat gtgggcagac
     4741 tatgaagact gttttaactt ttagtagtct cttacttctg aggaatgatg actcttgtct
     4801 ttgttcagac tgtgattcta cttttgaaag aattggggaa gagaactgtc caaattgtat
     4861 gaaaacagag ttgtcaacaa agtgtcaaga ttgtcaactt tggtgtaaag agggagttga
     4921 agtcagtcat agagcgattt ttacttacaa tcaagctatg aaggattttt tcagtcggta
     4981 taagtttgat ggagacttcc tgttaagaaa agttttcgct tcatttttaa gtgaggagtt
     5041 gaaaaagtac aaagagtatc aatttgttgt aattccccta agtcctgata gatatgctaa
     5101 tagaggattt aatcaggttg agggcttggt agaggcagca ggctttgagt atctggattt
     5161 attagagaaa agagaagaga gagccagttc ttctaaaaat cgttcagagc gcttggggac
     5221 agaacttcct ttctttatta aaagtggagt cactattcct aaaaaaatcc tacttataga
     5281 tgatatctat actacaggag caactataaa tcgtgttaag aaactgttgg aagaagctgg
     5341 tgctaaggat gtaaaaacat tttcccttgt aagatgagga aaaaatttgc aaaaatagaa
     5401 tgaaagcgtt ataataaaat cataaaaaca aaaatgttta gaaagaaggt actcatatga
     5461 ttaaatatag tatccgtggt gaaaacctag aagtaacaga agcaattcgt gattatgtag
     5521 tttctaaact cgaaaagatc gaaaagtact tccaaccaga acaagagttg gatgcccgaa
     5581 ttaacttaaa agtttatcgt gaaaaaacgg ctaaagtgga agtaacgatt ccgcttggat
     5641 ctattactct tcgcgcagaa gatgtatctc aagatatgta tggttcaatt gaccttgtaa
     5701 ctgataaaat tgaacgtcag attcgtaaaa ataaaacaaa aatcgagcgt aaaaataaaa
     5761 ataaggtagc aactggtcaa ttatttacag atgctttggt ggaagattca aatattgtcc
     5821 agtctaaagt tgttcgttca aaacaaattg atttaaaacc aatggatttg gaagaagcaa
     5881 ttctacaaat ggatttattg gggcatgatt tctttatcta tgtggatgtt gaagatcaga
     5941 caaccaatgt gatttatcgt cgtgaggatg gcgaaattgg tttgttagag gttaaagaat
     6001 cttaatttca atatgtgtaa aggtaggttt actgaattgt aaactacctt tttcttataa
     6061 ttgatagaat tactgattac acttttaaaa agtcgctttt tgaggggtat tatggtataa
     6121 tgggtgccaa gaggttttga atgaaaaaat tttatgtaag tccaattttt cctattctag
     6181 taggattgat tgcgtttgga gtcttatcca ctttcattat ttttgttaat aataatctgt
     6241 tgacggtttt aattttgttt ctttttgtag gaggctatgt ttttttattt aagaaactga
     6301 gagtgcatta tacaaggagt gatgtagaac agatacagta tgtaaaccac caagcggaag
     6361 aaagtttgac atctctattg gaacaaatgc ctgtaggtgt tatgaaattg aatttatctt
     6421 ctggagaggt tgagtggttt aatccctatg ctgaattgat tttgaccaag gaagatggtg
     6481 attttgattt agaagctgtt caaacgatta tcaaggcttc agtaggaaat ccgtctactt
     6541 atgccaagct tggtgagaag cgttatgctg ttcatatgga tgcttcttcc ggtgttttgt
     6601 attttgtaga tgtatccagg gaacaagcca taacagatga attggtaaca agtcgaccag
     6661 tgattgggat tgtctctgtg gataattatg atgatttgga ggatgcaact tctgagtcag
     6721 atattagtca aatcaatagt tttgtagcta attttatatc agagttttca gaaaaataca
     6781 tgatgttttc tcgtcgggta agtatggatc gattttatct atttactgac tacacggtgc
     6841 ttgagggctt gatgaatgat aaattttctg ttattgatgc tttcagagaa gagtcgaaac
     6901 agagacagtt gcccttgacc ttaagtatgg gattttctta tggcgatgga aatcatgatg
     6961 agatagggaa agttgctttg ctcaatttga acttggctga agtacgtggt ggcgaccagg
     7021 tggttgttaa ggaaaacgac gaaacgaaaa atccagttta ttttggtggt gggtctgctg
     7081 cttcaatcaa gcgtacacgg actcgtacgc gcgctatgat gacagctatt tcagataaga
     7141 ttcggagtgt agatcatgtt tttgtagtcg gtcacaaaaa tttagacatg gatgctttgg
     7201 gctctgctgt aggtatgcag ttgttcgcca gcaatgtgat tgaaaatagc tatgctcttt
     7261 atgatgaaga acaaatgtct ccagatattg aacgagctgt ttcattcata gaaaaagaag
     7321 gagttacgaa gttgttgtct gttaaggatg caatggggat ggtgaccaat cgttctttgt
     7381 tgattcttgt agaccattca aagacagcct taacattatc aaaagaattt tatgatttat
     7441 ttacccaaac cattgttatt gaccaccata gaagggatca ggattttcca gataatgcgg
     7501 ttattactta tatcgaaagt ggtgcaagta gtgccagtga gttggtaacg gaattgattc
     7561 agttccagaa ttctaagaaa aatcgtttga gtcgtatgca agcaagtgtc ttgatggctg
     7621 gtatgatgtt ggatactaaa aatttcacct cgcgagtaac tagtcggaca tttgatgttg
     7681 ctagctatct cagaacgcgc ggaagtgata gtattgctat ccaggaaatc gctgcgacag
     7741 attttgaaga atatcgtgag gtcaatgaac ttattttaca ggggcgtaaa ttaggttcag
     7801 atgtactaat agcagaggct aaggacacga aatgctatga tacagttgtt attagtaagg
     7861 cagcagatgc catgttagcc atgtcaggta ttgaagcgag ttttgttctt gcgaagaata
     7921 cacaaggatt tatctctatc tcagctcgaa gtcgtagtaa actgaatgta caacggatta
     7981 tggaagagtt aggcggtgga ggccacttta atttggcagc agctcaaatt aaagatgtaa
     8041 ccttgtcaga agcaggtgaa aaactgacag aaattgtatt aaatgaaata aaggaaaagg
     8101 agaaagaaga atgaaagtaa tctttttagc agatgttaaa ggaaaaggta aaaaaggcga
     8161 aattaaggaa gtaccaacag ggtatgcgca aaactttctt atcaaaaaga atctagccaa
     8221 agaagcgact gctcaagctg taggtgaact tcgtggtaaa caaaaatcgg aagaaaaagc
     8281 tcacgctgag atgattgcag aagggaaagc aattaaagca caacttgaag cagaagaaac
     8341 tgttgtagaa tttgttgaaa aagttggtcc agatggtcgt acctttggtt ctattaccaa
     8401 taagaagatt gcagaagaat tacaaaagca atttggaatt aagattgata aacgtcatat
     8461 tcaagtacaa gctccgattc gagcggttgg tttgattgat gtgccagtga aaatctatca
     8521 agatatcaca agtgtaatca atcttcgtgt gaaagaagga taagtttaca ccttcttgac
     8581 aagattgtaa aaggaaggga agtctgatgg cagaagtaga agagttacga gtacaacctc
     8641 aagatatctt agctgagcaa tccgttttag gggctatctt tattgatgag agtaaacttg
     8701 tttttgtgcg agaatacatt gagtctcggg acttttttaa gtatgcccat cgtttgattt
     8761 tccaagccat ggtcgattta tccgatcgtg gtgatgccat agatgcaaca acggttcgta
     8821 ctatccttga taatcaaggt gatttacaga atattggtgg cttgtcttac ttggttgaga
     8881 ttgtcaattc tgtgccaact tctgctaatg cggagtatta tgctaagatt gttgcagaaa
     8941 aagcaatgct acgtcgttta attgccaagt tgacagagtc tgtcaaccaa gcttacgaag
     9001 cgtcacaacc agctgatgaa attattgctc aggcagaaaa agggctgatt gatgtcagtg
     9061 aaaatgcaaa tcgaagcggg tttaagaaca ttcgagatgt gttgaatctc aactttggaa
     9121 atctggaagc tcgctcgcaa caaacgaccg atattacagg tattgcgaca ggttatcgtg
     9181 atttggatca tatgacaaca ggacttcatg aggaggagtt gattatctta gcagctcgtc
     9241 cagcagttgg taagacagca tttgccttga atatcgctca gaatattggg actaagttgg
     9301 acaaaacggt tgctattttt tcactcgaaa tgggtgcgga aagcttggta gaccgtatgt
     9361 tagctgcaga aggcttggtg gagtcacatt ctatccgtac agggcaattg acagatgagg
     9421 agtggcaaaa atatactatt gctcagggta atctagctaa cgccagtatc tatatcgatg
     9481 atacgccagg tattcggatt acagagattc gttctcgttc tcgtaaattg gctcaagaaa
     9541 ctggaaatct tggtttgatt ttgatagact atttgcaact tatcacggga actggtcgag
     9601 aaaatcgtca acaagaagtt tctgaaattt ctcgtcagtt gaaaatacta gccaaggaat
     9661 tgaaggttcc agtaatcgct ctgagtcagc tttctcgtgg tgtagaacaa cgtcaggaca
     9721 agagaccggt cttgtctgat attcgtgaat ctgggtctat tgagcaggac gctgatatcg
     9781 tagcttttct ctatcgcgat gactactatg aacgtggtgg tgaagaagag gagggtatcc
     9841 caaataataa ggtggaagtt attatcgaga aaaaccgtag tggagctcgt ggaacagtgg
     9901 aattgatttt ccaaaaagaa tacaataaat tttcaagtat ctcaaagagg gaggcataag
     9961 atgtcagatg catttacaga tgtagccaag atgaaaaaaa tcaaagaaga aatcaaggca
    10021 catgagggac aagtcgtaga aatgactttg gagaatggtc gtaagcgcca aaaaaataga
    10081 ttgggtaagc taattgaagt ttatccatcc ctatttattg tggagtttgg ggatgtggaa
    10141 ggagataaac aagttaatgt ttacgttgaa tcctttactt actcagatat tcttacagaa
    10201 aagaatttga ttcattatct tgactaaagt gagaaatttt ctcacttttt cttttttctc
    10261 cgaataattt aggtgaaggc aatcatcgct ttatattatt ttttaaggag gaagaatgaa
    10321 aattttaccg tttatagcaa gaggaacaag ttattacttg aagatgtcag ttaaaaagct
    10381 tgttcctttt ttagtagtag gattgatgct agcagctggt gatagtgtct atgcctattc
    10441 cggaggaaat ggatcgattg cgcgtgggga tgattatcct gcttattata aaaatgggag
    10501 ccaggagatt gatcagtggc gcatgtattc tcgtcagtgt acttcttttg tagcctttcg
    10561 tttgagtaat gtcaatggtt ttgaaattcc ggcagcttat ggaaatgcga atgaatgggg
    10621 acatcgtgct cgtcgggaag gttatcgtgt agataataca ccgacgattg gttccattac
    10681 ttggtctact gcaggaactt atggtcatgt tgcctgggtg tcaaatgtaa tgggagatca
    10741 gattgagatt gaggaatata actatggtta tacagaatcc tataataaac gaattataaa
    10801 agcaaacacg atgacaggat ttattcattt taaagatttg gatggtggca gtgttgggaa
    10861 tagtcaatcc tcaacttcaa caggcggaac tcattatttt aagaccaagt ctgctattaa
    10921 aactgaaccc ctagttagtg caactgtgat tgattactat tatcctggag agaaggttca
    10981 ttatgatcaa attctcgaaa aagacggcta caagtggttg agttatactg cctataatgg
    11041 aagctatcgt tatgttcaat tggaggctgt gaataaaaat cctctaggta attctgttct
    11101 ttcttcaaca ggtggaactc attattttaa gaccaagtct gctatcaaaa ctgaacccct
    11161 agttagtgca actgtgattg attactatta tcctggggag aaggttcatt atgatcagat
    11221 acttgaaaaa gacggctaca agtggttgag ttatacggct tataacggaa gtcgtcgcta
    11281 tatacagcta gagggagtga cttcttcaca aaattatcag aatcaatcag gaaatatctc
    11341 tagctatgga tccaataata gttcaactgt cggttggaag aaaataaatg gtagttggta
    11401 tcatttcaaa tcaaatggtt ctaaatcaac aggatggctg aaagatggtt ctagctggta
    11461 ttatttgaaa ttatctggtg aaatgcagac aggatggtta aaggagaatg gctcgtggta
    11521 ttatctgggt agttcagggg caatgaaaac aggctggtac caggtctctg gtaagtggta
    11581 ttattcttac tcttcaggcg ccttagctgt taatacgacg gtggatggct acagagtaaa
    11641 cagtgatgga gaacgagtat agaaaattgg agtaggagaa atttcctgct cttttctgta
    11701 agcagtttcc ttgacatttt tcttattttg cgctatcata tatacatata atatatggga
    11761 gtctgtgtac agtctgagag gaagtgttaa acttcgaccg cacctgatct gggtaatgcc
    11821 agcggaggga acgatactta gtctaatttt gcaccttttc catgtatggt aaaggttttt
    11881 ctttttttaa aaaggaaaac gagaagagga ggttcttatg aaagcaagca ttgccttgca
    11941 agttttaccc ctggcacagg ggattgatcg gatagctgtt attgatcagg tcattgctta
    12001 tctgcaaact caagaagtga cgatggtagt gacaccattt gaaacggtct tggaagggga
    12061 gtttgatgag cttatgcgca ttctaaaaga agcgctggaa gtggcagggc aggaggcaga
    12121 caatgtcttt gccaatgtca aaataaatgt aggagagatt ttaagtattg atgagaaact
    12181 tgagaagtat actgagacga cacattagtc tattgggctt tctcggagtc ttgtcaatct
    12241 ggcagttagc aggttttctt aaacttctcc ccaagtttat cctgccgaca cctcttgaaa
    12301 ttctccagcc ctttgttcgt gacagagaat ttctctggca ccatagctgg gcgaccttga
    12361 gagtggcttt actggggctg attttgggag ttttgattgc ctgtcttatg gctgtactca
    12421 tggatagttt gacttggctc aatgacctga tttaccctat gatggtggtc attcagacca
    12481 ttccgaccat tgccatagct cctatcctgg tcttgtggct aggttatggg attttgccca
    12541 agattgtctt gattatctta acgacaacct ttcccatcat cgtcagcatt ttggacggtt
    12601 ttaggcattg cgacaaggat atgctgacct tgtttagtct gatgcgggcc aagccttggc
    12661 aaatcctgtg gcattttaaa attccagtca gcctgcccta cttttatgct ggtctgaggg
    12721 tcagtgtctc ctacgctttt atcacaacag tggtatctga gtggttggga ggctttgagg
    12781 ggcttggtgt ttatatgatt cagtccaaga aattgtttca gtatgatacc atgttcgcta
    12841 ttattattct ggtatcgatt atcagtcttt tgggtatgaa gttggtcgat attagtgaaa
    12901 aatatgttat taaatggaaa cgttcgtaga attagaatgt ttctgaaaaa gaaaagagga
    12961 aatcaaaatg aagaaaacat ggaaagtgtt tttaacgctt gtaacagctc ttgtagctgt
    13021 tgtgcttgtg gcctgtggtc aaggaactgc ttctaaagac aacaaagagg cagaacttaa
    13081 gaaggttgac tttatcctag actggacacc aaataccaac cacacagggc tttatgttgc
    13141 caaggaaaaa ggttatttca aagaagctgg agtggatgtt gatttgaaat tgccaccaga
    13201 agaaagttct tctgacttgg ttatcaacgg aaaggcacca tttgcagtgt atttccaaga
    13261 ctacatggct aagaaattgg aaaaaggagc aggaatcact gccgttgcag ctattgttga
    13321 acacaataca tcaggaatca tctctcgtaa atctgataat gtaagcagtc caaaagactt
    13381 ggttggtaag aaatatggga catggaatga cccaactgaa cttgctatgt tgaaaacctt
    13441 ggtagaatct caaggtggag actttgagaa ggttgaaaaa gtaccaaata acgactcaaa
    13501 ctcaatcaca ccgattgcca atggcgtctt tgatactgct tggatttact acggttggga
    13561 tggtatcctt gctaaatctc aaggtgtaga tgctaacttc atgtacttga aagactatgt
    13621 caaggagttt gactactatt caccagttat catcgcaaac aacgactatc tgaaagataa
    13681 caaagaagaa gctcgcaaag tcatccaagc catcaaaaaa ggctaccaat atgccatgga
    13741 acatccagaa gaagctgcag atattctcat caagaatgca cctgaactca aggaaaaacg
    13801 tgactttgtc atcgaatctc aaaaatactt gtcaaaagaa tacgcaagcg acaaggaaaa
    13861 atggggtcaa tttgacgcag ctcgctggaa tgctttctac aaatgggata aagaaaatgg
    13921 tatccttaaa gaagacttga cagacaaagg cttcaccaac gaatttgtga aataatgaca
    13981 gaaattagac tagagcacgt cagttatgcc tatggtcagg agaggatttt agaggatatc
    14041 aacctacagg tgacttcagg cgaagtggtt tccatcctag gcccaagtgg tgttggaaag
    14101 accaccctct ttaatctaat cgctgggatt ttagaagttc agtcagggag aattgtcctt
    14161 gatggtgaag aaaatcccaa ggggcgcgtg agttatatgt tgcaaaagga tctgctcttg
    14221 gagcacaaga cggtgcttgg aaatatcatt ctgcccctct tgattcaaaa ggtggataag
    14281 gcagaagcta tttcccgagc ggataaaatt cttgcgacct tccagctgac agctgtaaga
    14341 gacaagtatc ctcatgaact tagcggtggg atgcgccagc gtgtagcctt actccggacc
    14401 tacctttttg ggcacaagct ctttctctta gatgaggcct ttagcgcctt ggatgagatg
    14461 acaaagatgg aactccacgc ttggtatctt gagattcaca agcagttgca gctaacaacc
    14521 ctgatcatca cgcatagtat tgaggaggcc ctcaatctca gcgaccgcat ctatatcttg
    14581 aaaaatcgcc ctgggcagat tgtttcagaa attaaactag attggtctga agatgaggac
    14641 aaggaagtcc aaaagattgc ctacaaacgt caaattttgg cggaattagg cttagataag
    14701 tagaaaaata gggagttggt gaagattatc ctttaccagc gccctttttc ttttaaaaat
    14761 gagaaaattt cggtataata gtcaaacaag gtcaaggttt aaagagagag gtgggtttgt
    14821 tatgagattt aaaaatacat cggatcatat tgaggcctac atcaaggcga ttttagatca
    14881 atctggtatc gtggagttgc aacggagtca gttggcagat acctttcagg ttgttcctag
    14941 tcagattaac tacgtgatca agacacgctt tacggaaagt agaggctact tggttgaaag
    15001 taagcgtggt ggcggaggct acattcgtat aggacggatt gagttttcta gtcatcatga
    15061 aatgctccgg gagctgcttt actcgattgg tgagcgagtc agtcaagaaa tttatgagga
    15121 tattctccag cttttggttg agcaggaatt gatgaccaag caggagatga atttgctaga
    15181 atcagtagct ttggatcgcg ttttaggaga agaagctcca gttgttcgag caagcatgct
    15241 acgtcagatc atacaagagg tagatagaaa agggaagtaa gatgaactat tcaaaagcat
    15301 tgaatgaatg tatcgaaagt gcctacatgg ttgctggaca ttttggagct cgttatctag
    15361 agtcttggca cttgttgatt gccatgtcta atcacagtta tagtgtagca ggggcaactt
    15421 taaatgatta tccgtatgag atggaccgtt tagaagaggt ggctttggaa ctgactgaaa
    15481 cggactatag ccaggatgaa acctttacgg aattgccgtt ctcccgtcgt ttgcaggttc
    15541 tttttgacga agcagagtat gtagcgtcag tggtccatgc taaggtgcta gggacagagc
    15601 acgtcttcta tgcgattttg catgatggca atgccttggc aactcgtatc ttggagaggg
    15661 ctggtttttc ttatgaagac aagaaagatc aggtcaagat tgctgccctt cgtcgaaatt
    15721 tagaagaacg ggcaggctgg actcgtgaag acctcaaggc tttacgccaa cgccatcgta
    15781 cagtagctga caagcaaaat tctatggcca atatgatggg catgccgcag actcctagtg
    15841 gtggtcttga ggactatacg catgatttga cagagcaagc gcgttctggc aagttagaac
    15901 cagtcatcgg tcgggacaag gaaatctcac gtatgattca aattttgagc cgtaagacta
    15961 agaacaaccc tgtcttggtt ggggatgctg gtgtcgggaa aacagctctg gcccttggtc
    16021 ttgcccagcg tattgctagt ggtgacgtgc ctgcggaaat ggctaagatg cgcgtgttag
    16081 aacttgattt gatgaatgtc gttgcaggga cacgcttccg tggtgacttt gaagaacgca
    16141 tgaataatat catcaaggat attgaagaag atggccaagt catcctcttt atcgatgaac
    16201 tccacaccat catgggttct ggtagcggga ttgattcgac tctggatgcg gccaatatct
    16261 tgaaaccagc cttggcgcgt ggaactttga gaacggttgg tgccaccact caggaagaat
    16321 atcaaaaaca tatcgaaaaa gatgcggctc tttctcgtcg tttcgctaaa gtgacgattg
    16381 aagaaccaag tgtggcagat agtatgacta ttttacaagg tttgaaggcg acttatgaga
    16441 aacatcaccg tgtacaaatc acagatgaag cggttgaaac agcggttaag atggctcatc
    16501 gttatttaac cagtcgtcac ttgccagact ctgctatcga cctcttggac gaagcagcag
    16561 caacagtgca aaataaggca aagcatgtaa aagcagacga ttcagatttg agtccagctg
    16621 acaaggccct gatggatggc aagtggaaac aggcagccca gctaatcgca aaagaagagg
    16681 aagtacctgt ctacaaagac ttggtgacag agtctgatat tttgaccacc ttgagtcgct
    16741 tgtcaggaat cccagttcaa aaactgactc aaacggatgc taagaagtat ttaaatcttg
    16801 aagcagaact ccataaacgt gtcatcggtc aagatcaagc tgtttcaagt attagtcgtg
    16861 ccattcgccg taatcagtca ggaattcgca gtcacaagcg tccgatcggt tcctttatgt
    16921 tcctaggacc cacaggtgtc ggtaagaccg agttggccaa ggctttggca gaagttctct
    16981 ttgatgacga atcagccctt atccgctttg atatgagtga gtatatggag aaattcgcag
    17041 ccagccgtct aaatggagct cctccaggct atgtgggtta cgaagaaggt ggggagctga
    17101 cagagaaggt tcgcaataaa ccctattccg ttctcctctt tgatgaggta gagaaggccc
    17161 acccagatat ctttaatgtt ctcttgcagg ttctggatga cggtgtcttg acagatagca
    17221 agggacgcaa ggtcgatttt tcaaatacca ttatcattat gacatcgaat ctaggtgcga
    17281 ctgcccttcg tgatgataag actgttggtt ttggggctaa ggatattcgt tttgaccagg
    17341 aaaatatgga aaaacgcatg tttgaagaac tgaaaaaagc ttatagaccg gaattcatca
    17401 accgcattga tgagaaggtg gtcttccata gcctatctag tgaccatatg caggaagtgg
    17461 tgaagattat ggtcaagcct ttagtggcaa gtttggctga aaaaggcatt gacttgaaat
    17521 tacaagcttc agctctgaaa ttgttagcaa atcaaggata tgacccagag atgggagctc
    17581 gcccacttcg cagaaccctg caaacagaag tggaggacaa gttggcagaa cttcttctta
    17641 agggagattt agtggcaggc aacacactta agattggtgt caaagcaggc cagttaaaat
    17701 ttgatattgc ataaaagaat aaaagtatca gcatctgacc ataagtcaca gtggagtgaa
    17761 actcaatgaa aatcaaagag caaactaggc agctagctgc aggttgctca aaacactgtt
    17821 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta tgaaactaaa
    17881 acctatagct tctaaacgat ccgtggtttt caccattcaa cacaaaattc atatgtttat
    17941 taccctccgt cgtatttgtc ttagagcgtg tgtagtagaa aaagagcagt cttatctgaa
    18001 atttttattc tttcaaaaga gacctgtttc ttttttgcat gtcaaatccg ttctagctgg
    18061 tatttgaaaa atcaaactaa tattcaatga aaatcaaaga acaaactagg aagctagccg
    18121 caggttgctc aaaacactgt tttgaggttg tagatagagc tgacgtggtt tgaagagatt
    18181 ttcgaagagt ataagctgca agatgaatga ttttcttgta ttgacgttgt tgttgacaaa
    18241 aagtagcgga taaatgaaat ccattccatt atcatagatg ataggctggt aggaaatttt
    18301 caaatagcat acaggaaata gatgtatgga gttctggtag tagaaaggga gagagatgaa
    18361 cattttagtt gcagatgacg aggaaatgat tagagaagga attgcagcat ttctgacaga
    18421 agagggttat catgtcatta tggctaagga tggacaagag gtcttggaaa aatttcaaga
    18481 tctccctatc catctcatgg tactggattt aatgatgcct aggaagagtg gttttgaagt
    18541 gttaaaagaa atcaatcaaa agcacgatat tcctgtcatc gtcttgagtg ctctgggaga
    18601 tgaaactact cagtcacagg tatttgatct ctatgctgat gatcatgtga caaaaccttt
    18661 ttctttggta ctgcttgtca agcgtattaa ggcgcttatc agacgttact acgtcataga
    18721 ggatctttgg cgatatcagg atgtaacagt ggattttacc tcttacaaag cacattataa
    18781 aaatgaagaa attgatctca aaccaaagga attactggta ctaaagtgtt tgattcagca
    18841 taaaaatcaa gttttaagta gagagcagat attggaagaa atttcaaaag atgtagctga
    18901 tttaccttgt gatagggtcg ttgatgtcta tattcgtact cttcgcaaaa aattagcttt
    18961 agattgtatc gtgactgtga aaaatgttgg gtataagatt agcttatgat aaaaaatcct
    19021 aaattattaa ccaagtcttt tttaagaagt tttgcaattc taggtggtgt tggtctagtc
    19081 attcatatag ctatttattt gacctttcct ttttattata ttcaactgga gggggaaaag
    19141 tttaatgaga gcgcaagagt gtttacggag tatttaaaga ctaagacatc tgatgaaatt
    19201 ccaagcttac tccagtctta ttcaaagtcc ttgaccatat ctgctcacct taaaagagat
    19261 attgtagata agcggctccc tcttgtgcat gacttggata ttaaagatgg aaagctatca
    19321 aattatatcg tgatgttaga tatgtctgtt agtacagcag atggtaaaca ggtaaccgtg
    19381 caatttgttc acggggtaga tgtctacaaa gaagcaaaga atattttgct tttgtatctc
    19441 ccatatacat ttttggttac aattgctttt tcctttgttt tttcttattt ttatactaaa
    19501 cgcttgctca atcctctttt ttacatttca gaagtgacta gtaaaatgca agatttggat
    19561 gacaatattc gttttgatga aagtaggaaa gatgaagttg gtgaagttgg tgaagttgga
    19621 aaacagatta atggtatgta tgagcacttg ttgaaggtta ttcatgagtt ggaaagtcgt
    19681 aatgagcaaa ttgtaaaatt gcaaaatcaa aaggtttcct ttgtccgcgg agcatcacat
    19741 gagttgaaaa cccctttagc cagtcttaga attatcctag agaatatgca gcataatatt
    19801 ggagattaca aagatcatcc aaaatatatt gcaaagagta taaataagat tgaccagatg
    19861 agccacttat tggaagaagt actggagtct tctaaattcc aagagtggac agagtgtcgt
    19921 gagaccttga ctgttaagcc agttttagta gatattttat cacgttatca agaattagct
    19981 cattcaatag gtgttacaat tgaaaatcaa ttgacagatg ctaccagggt cgtcatgagt
    20041 cttagggcat tggataaggt tttgacaaac ctgattagta atgcaattaa atattcagat
    20101 aaaaatgggc gcgtaatcat atccgagcaa gatggctatc tctctatcaa aaatacatgt
    20161 gcgcctctaa gtgaccaaga actagaacat ttatttgata tattctatca ttctcaaatc
    20221 gtgacagata aggatgaaag ttccggtttg ggtctttaca ttgtgaataa tattttagaa
    20281 agctatcaaa tggattatag ttttctccct tatgaacacg gtatggaatt taagattagc
    20341 ttgtagacag attagttttt tattaaagtt catatagggt taacataagt gtgttattct
    20401 ttgtgtagat aaaagaaagg atactaatat ggtattagcg attattttag taacattcct
    20461 tattcgattg atttttttaa agcgttcgat agagaatgag aaacgaatcc ttagcaatgg
    20521 cgggaaagaa tttggagttg agaatacaaa acgattaact atggctcata ttgtttttta
    20581 tctctcttgc ttggttgagg caatggtgca caagacaatt tttgatagca tgggcatggt
    20641 tggtttagtc ttgcttattt tttctatgct gatgttaatg ttggtgattc acttgttggg
    20701 agatatttgg acagtgaagc ttatgcttgt caataatcac aaatatgtag atcatatctt
    20761 gtttaggaca gtaaaacatc ctaattactt tttaaatatt cttcctgagt tgattggctt
    20821 gaccttgttg agtcatgcct atatgacttt tgttttagtt tttccagttt atgcagttat
    20881 tttgtatcga cgaatagctg aagaggaaaa gctattacat gaagttataa tcccaaatgg
    20941 aagcataaag agataaatac aaaattcgat ttatatacag ttcatattga agtgatatag
    21001 taaggttaaa gaaaaaatat agaaggaaat aaacatgttt gcatcaaaaa gcgaaagaaa
    21061 agtacattat tcaattcgta aatttagtat tggagtagct agtgtagtag ttgctagctt
    21121 gttcttagga ggagtagttc atgcagaaga ggttggtggt aggaataccc ccacggttac
    21181 atctagtggg caagatatat cgaagaagta tgctgatgaa gtcgagtcgc atctaaaaaa
    21241 aatattgagt gagatccaaa cacaattaga tagaaaacga cataccgaaa ctgtagcgct
    21301 aattaacgag ttgcaaggca ttaagaaaac gtatttgtat aatttaaatg ttttaaaaga
    21361 gaagtcgaaa gttgagttgc cgtcaaaaat aaaagcaaag ttagacgtag cttttgatca
    21421 gtttaaaaaa gatacattga aaccaggaga aaaggtagca gaagctcaga agaaagttgc
    21481 agaagctaag aaaaaagccg aggatcaaaa agaagaagat cgccgtaact acccaaccaa
    21541 tacttacaaa acgcttgaac ttga
//
LOCUS       DAVMHG010000022        21528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMHG010000022 DAVMHG010000000
VERSION     DAVMHG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.022"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYR08_000648"
     CDS             complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYR08_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV3466218.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2205..3074)
                     /locus_tag="VYR08_000649"
     CDS             complement(2205..3074)
                     /locus_tag="VYR08_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3466219.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3298..3906)
                     /locus_tag="VYR08_000650"
     CDS             complement(3298..3906)
                     /locus_tag="VYR08_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3466220.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(3864..4970)
                     /locus_tag="VYR08_000651"
     CDS             complement(3864..4970)
                     /locus_tag="VYR08_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV3466221.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5081..5335)
                     /locus_tag="VYR08_000652"
     CDS             complement(5081..5335)
                     /locus_tag="VYR08_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466222.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5693..6139)
                     /locus_tag="VYR08_000653"
     CDS             complement(5693..6139)
                     /locus_tag="VYR08_000653"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3466223.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6212..6537)
                     /locus_tag="VYR08_000654"
                     /pseudo
     CDS             complement(6212..6537)
                     /locus_tag="VYR08_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6771..8489
                     /locus_tag="VYR08_000655"
     CDS             6771..8489
                     /locus_tag="VYR08_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV3466224.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            8600..8947
                     /locus_tag="VYR08_000656"
     CDS             8600..8947
                     /locus_tag="VYR08_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3466225.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9226..10062)
                     /locus_tag="VYR08_000657"
     CDS             complement(9226..10062)
                     /locus_tag="VYR08_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466226.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10075..10704)
                     /locus_tag="VYR08_000658"
     CDS             complement(10075..10704)
                     /locus_tag="VYR08_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3466227.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10714..11355)
                     /locus_tag="VYR08_000659"
     CDS             complement(10714..11355)
                     /locus_tag="VYR08_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3466228.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11501..12730)
                     /locus_tag="VYR08_000660"
     CDS             complement(11501..12730)
                     /locus_tag="VYR08_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV3466229.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12720..13886)
                     /locus_tag="VYR08_000661"
     CDS             complement(12720..13886)
                     /locus_tag="VYR08_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3466230.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14030..15043
                     /locus_tag="VYR08_000662"
     CDS             14030..15043
                     /locus_tag="VYR08_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV3466231.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15088..15507)
                     /locus_tag="VYR08_000663"
     CDS             complement(15088..15507)
                     /locus_tag="VYR08_000663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV3466232.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15518..16924)
                     /gene="atpD"
                     /locus_tag="VYR08_000664"
     CDS             complement(15518..16924)
                     /gene="atpD"
                     /locus_tag="VYR08_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV3466233.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17010..17888)
                     /locus_tag="VYR08_000665"
     CDS             complement(17010..17888)
                     /locus_tag="VYR08_000665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV3466234.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(17904..19409)
                     /locus_tag="VYR08_000666"
     CDS             complement(17904..19409)
                     /locus_tag="VYR08_000666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV3466235.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19424..19960)
                     /locus_tag="VYR08_000667"
     CDS             complement(19424..19960)
                     /locus_tag="VYR08_000667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV3466236.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(19960..20454)
                     /gene="atpF"
                     /locus_tag="VYR08_000668"
     CDS             complement(19960..20454)
                     /gene="atpF"
                     /locus_tag="VYR08_000668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV3466237.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20468..21184)
                     /gene="atpB"
                     /locus_tag="VYR08_000669"
     CDS             complement(20468..21184)
                     /gene="atpB"
                     /locus_tag="VYR08_000669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV3466238.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21219..21419)
                     /locus_tag="VYR08_000670"
     CDS             complement(21219..21419)
                     /locus_tag="VYR08_000670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV3466239.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         6419 a         4645 c         3933 g         6531 t
ORIGIN      
        1 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct aacttaaata
       61 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      121 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      181 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      241 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      301 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      361 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      421 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      481 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaacg
      541 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      601 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      661 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      721 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      781 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
      841 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
      901 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
      961 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1021 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1081 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1141 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1201 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1261 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
     1321 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
     1381 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ttaatagaaa
     1441 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
     1501 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatcgttca
     1561 ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca
     1621 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
     1681 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
     1741 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagt aaaatgaaat
     1801 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
     1861 ggaggactat tccaacttca acccactata aagcagactt acaagatagg gaacccatct
     1921 ccgataatat tccgataata tatagtagaa tgaaaactgc acaatttgat tggagaattc
     1981 aaaccaattt ctcacaatgt tttagaaaca agggtctatt atttcaactg caacctacta
     2041 tagtccattg aagctggaat agcccactga gacttctaaa ccactgctgg agaggagttt
     2101 gtttagagtt tctttcattt ccctaaaaaa tcaaaaagag agaaccagtc tgatcctctc
     2161 ttacttatag atggtgctca ctcaaatgtt aaaggagcta tttcttagag ataaaggaat
     2221 ttgaatacag ctactgccgc aattgctgct gcgataggtg ctactactgg tacccaagaa
     2281 taccaccatt ttgaatcgcc tttatgctca ccaagaactg atttgggaag gaaagcatga
     2341 aggagacgtg gtcccaagtc acgggctggg ttcaaggcag gtcctgtagg tcctccaagt
     2401 gatgttacca aagccataac gaggaatcca agtgccaagt gagccacaga aagtcctgaa
     2461 gcagtgtgtg gagccacctg tgctttaata gccaagtcag aaaaatcaac tgtttgtcct
     2521 gcttctgttg ccttttgttt catgaattga agcacttcag caccaaagaa gtttttagtc
     2581 aaaccaagag ctgcaaagaa caaaacaaat gaaccaacaa actcattaat caaaccattg
     2641 acagttgctg catagcgact ttcttttgta ccatggtcaa tacttgaaat agttgagaaa
     2701 gttcccaaga tgttatttgg gttttcagtt ttcaagtaga atggacggta tgttgccaca
     2761 actaaggctt ggccaaagat agcccccaag acttgcgcga taatgtaagg taccacttgt
     2821 gcccaaggga aaagaccgct aactgcaagc cctagagtga aagcagggtt gatgtgattc
     2881 ccagatacgt taccaaacat caaggctggg atcataaccc ccataccata accaacagcg
     2941 atgacgatcc agccactttg gtgacctttc gtacctttaa gttcaacgtt ggcaactgca
     3001 ccattcccaa gaatgatcaa aatggcagtt cccaaaaatt cagtggcata cttcagtgcc
     3061 catgtgaaat ccatttgcta gattctcctt aaaatttttt agatattcct tattttatca
     3121 atttttaccc tatttagcaa ctgatttctg gaaatatttt taaaaaaata ggattcatag
     3181 tttcccagtt caacagactt gtaagcgttt ttattcttta cgaaagacct cgttctcaaa
     3241 accaggaaaa aagcccacat ctgtgagctt ttcctggttc ctagattcta aaatctatta
     3301 tttttcatct tcttttttct tgaaaaatcc taatcctaga ccagctaaca aggtcatgat
     3361 accagcacta gccaaggtag cattagcttc tgtacctgtg tttggcaatt cctctctctt
     3421 acctgtctca taagtcggaa cttctgggtc tggattcact ggagtttcag tttttggagt
     3481 acctggttct ggagttggtt tatctggtgt tggagtctct tgatttggta gttctgggtt
     3541 tggtgtcgga acacttggat gctctggatt tggttgtggg attggttttt ctggtgttga
     3601 taaacggtca taccttaccg ttatttcttt atcactagag tctgacgtaa cttcttgtga
     3661 ttcaactgtt ggaatatctg gatctttgta cttgtcaatc ttaccagata taacctcgtc
     3721 ccagtttcct gttgtccatt caccgtaggt tacaactccc gtgaccttgt tctcagtttt
     3781 tgtacggctt aaggttacag gttgaacaac atcttctttt acattttggt tcgtaacttt
     3841 atcaacgtaa tgaatgatac gcgttatagt cttcgtctca gtagaggttg ctgttttggg
     3901 aaccactgtt tcctcaacat tctcacggta gtaatagtca actgttgcac cgtcttctgg
     3961 tacgcatttg caggagttgc taccaaggtg tatgttgttt tccttgtgat aactcggtct
     4021 tctttgtcct cagttgttgt tttcccttca atagtttttg attctgtggt atactcagaa
     4081 cctatcgcta aatcagcttt tataacagac tctaccaact tctcttgcct accttcttta
     4141 tagtaattgg ctgttactgt agcagtggtt ggcgcttcgc tttctctata aactaaggtc
     4201 actgttctac cttcgcttac aatattccca gttaaacttg cagaatttgt atctgcttct
     4261 ttaaaagtat aatattttcc gtcagtagta gtcatgctac tgagtttttt atctgtgaca
     4321 ttatagctgg taccaatcag ttgtttttta ttggtaatgt aggttccgtc actttctttt
     4381 tctccaattc cagtatcatt tttcaatgat agcaaacgcc cttgttcatc aacatagcga
     4441 actttcacat tttctgagat tagttctgcc aattctgagg tttttttctt tttcttgatt
     4501 tcttcggtta ttttcccttt ctcttcttcg ggaatattta gttttggaat gattttttca
     4561 acaacggttc gtgatggttc cacagtatct tggatgactg aaaagtcagc tagaattggg
     4621 agattatact gaacacggtg actttgagtg tttactccta ctctttcatt attctctgaa
     4681 aatacttgta cggtataaga aacaacatct tttcctaata gaacatcccc agtagagaaa
     4741 tagccgcctt ttcctagttt gctatctcca gagcccactt ctttcctaat cttatcagat
     4801 agttttttac cagtcagtac attcgttcgc acaatccctt tgtctacccc tacaaagtgg
     4861 gagaactttt tgaactcttc agaaccagat ctagcccaac cattattaag ggcatttgct
     4921 tttgtatttg tattctctct caaaagtttg gcgattagaa ttatattcat cggcacttag
     4981 agttgctgct atatctgact cttgaatacc aacttcctta ctaccatttc tagcggcagt
     5041 atatgtgtat ttatctgttt ctacttctag ctgattatcc ttaacatgat aggtaatttt
     5101 ctcctggtat aagagtcttc agtagtttat atactctgtc atcatcttta gctttagaag
     5161 cagctgagtt tagccaagtt ggggtattag gagtatcttt atcagcctgt ttcaagacat
     5221 tattcaagtc atttgctagc ctattggtta tcctcactgt gaaagttttc ttccagtctg
     5281 aaccactttt attagactta gatatgctca tgaaatcctg gtcaacattc aacacaatat
     5341 cgccttgttt tttagcatct tccgttgctt ctgcttcaaa ttgatcatgg agtttttgtt
     5401 gctctggtgt atagtttttt tatttacacg atttaatgta ctatttttta ttttagtatt
     5461 aagaactcca ttaatacgat ttttaaactc tgcttcaaaa aatcccttaa tttgttggtg
     5521 cgcaagttca acattcaaac ctttcttata caagtcttca ttctgataca agtcttcatt
     5581 ctgaacgatt tcggctacac ggtgaatggt ttgattgtat accgtcttct cttaaccagc
     5641 agtatcctta gttttaatga ctactcgata ggttccttta gaaccgttat agtcatctag
     5701 ttttaaaaaa gcattccaaa cattcaaaaa tagttggaac ttcccttaat acatccgtca
     5761 ctttcttttt caagatagcc caagcttgct caataggatt caaatccggt gaataaggtg
     5821 gtagaggtaa gaaaaagtgt ttatcttgga tgcagagttc atccaaaatg ttcttgggat
     5881 gaaaaccagc attgcccatg acaataacat gaggtgtctt caaagcaggt aggagttgcg
     5941 ttttgaacca ctccacaaag aaatcgcttg tcatgctttt cttgtaaatc atgggagcta
     6001 taaactctcc gtctacttgt cctgcaacaa ttgaagttcg ctcaaaacga cgtccgctaa
     6061 tcttttcata gactttctcc cctctaggag cccctgcata aggacgatag agatagcggt
     6121 cgattcctgt ttcatcaata taaacaggtg ctaggtgctt taaactatta aaattcttaa
     6181 gaaataaggc tacttttctg ggtcttgttc cttaaagctc gtcatctttt tttaaagtga
     6241 ccttaatctg ctttaaagct gcccatactg aaggaacagc acaatcaaaa tgtgccgcaa
     6301 tttcccgtaa aaaagcatct ggatgagcct ctacaaaggc tttcaattcc tctaaaggaa
     6361 tctttcggtt tttgacgact cgctttttcc tctctatgtg ttcttgttca cgtctttctt
     6421 ttcccacgtg aagagagttc tgacgccaac accaaaaaac ttggctgcct cgacatggct
     6481 gtgcccctct ttgatgtaat ctaatgctcg ctgtttaaaa tcagtactat atgccatagc
     6541 tttattataa tatgcttgtt taaaactaag taactatatc aatgttttgt tatgagaaga
     6601 cttacttttt ttactgaaat tgaatttttc ctagcctcaa catcatataa ccccaaaatt
     6661 caatctgacc tggtttgaag agatgttcga agcagataag gttttactaa aactaaaaaa
     6721 aatgatataa tcaaactgat taaatgtttt aaaaataaag gagaatttga atgtcttacc
     6781 aagaaaatta ccagaaatgg gttgattttg tggagcttcc tgactacctt cgtcaagatt
     6841 tggaaaatat ggacgaaaaa actaaggaag atgccttcta tacaaatctt gaatttggta
     6901 ctgcaggtat gcgtggcttg gttggtgctg gtacaaaccg tatcaacatc tacgttgttc
     6961 gccaagctac tgaaggattg gctcgtttga ttgagtcaaa aggtggaaac gagaaagaac
     7021 gcggtgtagc aattgcctac gatagccgtc acttctcacc tgagtttgcc tttgaatctg
     7081 cggcagttct tgctaaacac ggcatcaaat cttacgtatt tgaaagcctt cgtccgactc
     7141 cagaactatc atttgcagtt cgtcatctca actgtttcgc aggtatcatg gtcacagcca
     7201 gccacaaccc tgctccattt aacggttaca aggtttacgg tgaagacggt ggacaaatgc
     7261 ctccacacga tgcggacgct ttgactactt atatccgtgc aatcgaaaac ccatttgcag
     7321 ttgaagttgc tgatgtggaa actgaaaaag cttctggctt gattgaagtt atcggcgaag
     7381 ctgttgacgt agaatacctt aaagaggtta aggacgtaaa catcaaccca gccttgattg
     7441 aagaatttgg taaagacatg aagattgtct acacaccact tcatggtact ggtgaaatgt
     7501 tggctcgtcg tgctcttgcc caagcaggat ttgactctgt tcaagttgtt gaagcgcaag
     7561 caactgctga cccagacttc tcaactgtaa catctccaaa cccagaaagc caagcagcct
     7621 ttgcccttgc tgaagaactt ggtcgtcaag ttggtgcaga tgttcttgtc gcaactgacc
     7681 cagacgctga ccgtgttggt gttgaagttc ttcaaaaaga tggtagctac ctcaaccttt
     7741 caggtaacca aatcggtgct atcatggcta aatacatctt ggaagctcac aaaaacgctg
     7801 gaactcttcc tgaaaatgcc gctctctgca aatctatcgt ttcaactgac ttggtaacga
     7861 agattgctga aagctacggc gcaactatgt tcaacgtctt gacaggtttc aaatttatcg
     7921 ctgaaaaaat ccaagaattc gaagaaaaac acaaccacac ttacatgatg ggatttgaag
     7981 aaagcttcgg ttacttgatt aaaccatttg tacgtgataa agatgccatc caagccgttc
     8041 ttgtcgttgc tgaacttgct gcctactacc gttctcgtgg tttgacactt gctgacggta
     8101 tcgaagaaat ctataaagaa tatggctact acgcagaaaa aacaatctct gttactcttt
     8161 caggtgtcga tggtgctgaa caaatcaaag cgattatggc taaattccgc aacaatgctc
     8221 caacagaatg gaacgcaaca gctatcactg tcgtagaaga cttcaaggca caaactgcta
     8281 ctgttgctga cggtactgtt acaaacttga caactcctcc aagtgatgtg ttgaaataca
     8341 cacttgctga cggttcatgg attgccgttc gcccttcagg tacagaacca aaaatcaagt
     8401 tctacattgc agttgtaggg gaaaccaacg aagaatcaca agctaagatt actaacatcg
     8461 aagcagaaat caatgcattt gtaaaataaa gacctataaa agatgagacc aaccaacctc
     8521 atcttttttc gtatcaaatc taagcaattt tagaaacttt atggcatact agacttatca
     8581 atgaaagcga ggtaatctca tggaacaatt tttagataac atcaaagacc ttgaagtcac
     8641 tacagttgtg cgtgcgcaag aagctcttga taaaaaagaa actgcaacct tctttatcgg
     8701 tcgcaaaact tgcccttact gccgtaaatt tgcaggtaca ttgtcaggtg tcgtagctga
     8761 aaccaaagct cacatttact tcatcaatag tgaagaacca agccaactca atgatttgca
     8821 agcattccgc tcacgttatg gaatcccaac tgtaccaggt tttattcata ttacagatgg
     8881 acaaatcaac gtccgttgcg actcttcaat gtcagcacaa gaaatcaaag atttcgcagg
     8941 attgtaaaaa atcagctaac aagtcttaaa atcacaaaaa cgcataatgt aaggtgcaaa
     9001 aaccttgata ttatgcgttt tattgttcct aaaacagtta catcagcatt tcattacaga
     9061 acattttaat tttggaagtc aaatcaattt ttaacaatgt tttagaaatc acagtgggct
     9121 attccatatt caattatcca gattcaatcg ctattatcgt tttcctagat tacaaaaaaa
     9181 actacgtttt gctaatcaaa acgtagtctc agccactatt ttatcttact gtccttcttt
     9241 tacttctttg gttgctacat cttctccaaa ccatttttgg ctgatttctt ggaacttgcc
     9301 gtccttgtaa agactagaaa aagcttcatt tatcttctta accaaggttg tatcttcctt
     9361 acgggatcca accgcaaaag cttctgtttc tagtccaact gtaaagacat tataatcgtt
     9421 taaaacacct tctgcttcta aataatagtt tgcatagaca cggtcaatca atagaccatc
     9481 aattcgatcg tttttcaaat caatcaaggc ttcattaaag gtttggtatt gattcgcttc
     9541 cttattagcg acaatattct tcaaaatttc tggatttgct tcaaagtccg cataaccaga
     9601 tgaaccagct tgagctccta atgtctttcc agtcatatcc tttgcagtcg tgataccaga
     9661 tgatttcttc gtaaccaata cctgctcatt cttcatatat gagttactga aagccacctt
     9721 ttcacggcgt tcgtctgtag cggaatagcc attccaaatc agatcaatcg ttccttttgt
     9781 caattcagct tctttcaaat cccaatcaat cggttgccaa tttaccgtga ttccgtattt
     9841 ttcaaaaaca gctgtagcta aatcaatatc aaatcctgca taagaaccat ctttctgagc
     9901 aaatcccatt ggaacaaaag tactatcaaa tccaatagta atagacttgt tagactggta
     9961 ctttgaccaa ttatctccac tagtttcgct agaatttttc ccacaagcta ctaagaacaa
    10021 agcagtcatc agactgacta atacaagcat ccattttttc atccattttt cctcctattt
    10081 aggttctact ttcaataata catctgcgat attttcagca aactgcaaat catgggtaac
    10141 cacaatctgg gtcatcccaa gttccctatt ttgcaagatt agcttctcca cttccaaacg
    10201 taattctgga tccagggcag aagttggttc atcgtagcca atgatttctg ggtcaatcat
    10261 catagcacgc gccaaagcca cccgctgctt ttgcccacca gatagtgaga aaggataggc
    10321 ctctgcgtgt cctcctagtc ctaactgttc caagagccca ctcgccttct tctcagcctc
    10381 ttcctgcttc attcccatgg tcttcacagg cgataaagtc aaattttcca gaactgatag
    10441 atgaggaaat agttgaaaat cttggaagac aaatcccagt aggttgcgct tctgcaattc
    10501 atccagctct aaaggttgtc cattataaaa gatttgccct gaatcaatgg tttcaagacc
    10561 tgcaagcata cgtaaaagag ttgtcttacc tccaccagaa ggtccaacga tagccaggat
    10621 ttgcttttca ggaatactta gactgaaatt agacaggatt tgtttgtctc caaagacttt
    10681 attgatattt cgtaattcta acatggcagc ctcctatcta taataactat acttcttctc
    10741 aacttttttg gaaataattg tcacaatccc aatcaaaatc aaataaatgg ctcctgccaa
    10801 gaacatagga actagactag catcgcggtt agcagctgtt cgactagcca agataaggtc
    10861 tgaaattccg agagcataga ccaaagaagt atccttgacc aaactcataa cttcattaaa
    10921 gacactagga agaacaatct tggtcacttg gggcaagata atatagcgca ctctgtcaaa
    10981 agggctaaac tttaagacct tggcagcctc atactgtcct cttggaatag tgtcaattcc
    11041 cccacggaaa atttctgcaa agtaagctgc atagttgaga acaaaggcaa taatagctgc
    11101 aggaaggcgg tctaaacgaa tcccaatact tgggagcaca taatagataa aaatcagttg
    11161 caagagtaag ggggttcctc gcataaccca gatgtaaatg ttaatcagat aatggagggg
    11221 cttccaatgg acttgcaagg caaaggcaat caaaacgccc aagggaatcg aaaatagcaa
    11281 gaccagtgca aagacctgta cagtcgtgct cgcaccattc agtaaactcg gtaatatctc
    11341 aaataaataa gacatactgc acctcctaaa agtaattgtt cctattatag catgaataaa
    11401 caagattacc aagtattttt gtaaaaagat tctaccatat acaaataaaa accgtagcta
    11461 tcaaaagaga taaactacag ttctatatgt ttaaacattc ttacaatctc tcaaacagtt
    11521 cttgcatttg cacatcatct ggaaccagtt ttaagtaagt gtgagcatgg acttttgctt
    11581 cttcaaaatg tcccaattca cgcaagagat agatatagtg ttccagaaat tctggattgt
    11641 ccttcaaatc tcctgtcaac tcttgataat gctcataagc agtatccaaa tcgtccattt
    11701 cttgataaga acgagcaatc atccacttgg tcaaaagatt ttctggctcc tcactctgca
    11761 agtctagaat atcctcataa cgctcctgct ccagataaat agtggctaaa cgaagcaaga
    11821 tttcttctgt atcctcagcg tcttcttttg cagtaaggag ataattttct gcaccactag
    11881 catcatgcaa ttcataagaa aattgtgaag cagctagcaa gaggcgagtt tcaaagggat
    11941 ttttctctaa tccttgctta gcgatacgca gggcttcttg aacttgatgt tccttatgta
    12001 aagcctgact gtacccatac tcatagcctt caaagtcagg agaaatggta tcaagctgct
    12061 taaagtagag ggtggctttt tgatattctt cttgatcaaa ataaagactg gccaactcaa
    12121 aagctgttaa gtcatcgtat tctaactcca gggctttttc taaaaactca gtagccgttt
    12181 caaatttccc taactgagca taggcaaagc caattcgttg ataggtggaa atgcccgttt
    12241 gctcataaat cgagcgatta tctaactggg cataggcttg aatagccgct tggtaatttt
    12301 ccaactcact atccaactct gccaaaccca atatcaagag agaatcctct gagtaggtca
    12361 aggcctccaa taatttctca cgtgccacat ctgtcaaacc ttccagctgg taaaggtctg
    12421 ccttcaaaac caaagacgag acataccagt cactgtcagc ttggatttcc tcaagatagg
    12481 taaaggcttc ttctatttga ccatcctcgc tagcaattgc agctagatta agatgaacct
    12541 ctggaaaatc ctctacaatt ttcaggtaaa tttccttggc ctgaggatag aaaccaatcc
    12601 cttcaagata agttgccaac tcatacagaa gattacttga atcattttct aaagctttgg
    12661 cgaaataatg ctcagcctta gttaaatctt gctcctccaa agcctgtaac atctgttgac
    12721 tattgttcac tcttgacttc ctcaccctct aattcatata gaccactgac taccttatac
    12781 cattcgaaaa tggctgaaat gacaacctta gcagaggcat aaaccggaat accaagtaaa
    12841 actccccaga taccaaacat agatcctgaa gttaacaaaa caaagagaac attaataggg
    12901 tggatgttta attgacttcc caaaatcaat ggagagacaa aacggccttc aatagtttgt
    12961 tctacgataa agacaatcac tactttcaaa agcatgactg gaccagcaat caaacccaat
    13021 actagagcag gaagcatggc tagaaagcta ccaagataag ggaccagatt taaaatacca
    13081 gcagtaaccc ccagcgtaac cgcatagcgt agaccaataa tcttgaagaa gatgataaac
    13141 attactgcta caataatagc cactgtcact tgccctcgaa catagttgga caactgttga
    13201 ttcacatctg atagaacttg tccaacaggt tccttcaatt ttcttggaat gaattgggtc
    13261 aaatagttac gcaagccttt cccatcacgc aagagataaa agagcatgaa aggaacgata
    13321 atcaaggcaa caatcacttg agaagccccg ctaataaagg cactcaccca gttgactgcc
    13381 tgagatgaaa ccttacttgc caaaactgta gcctggctag aaaaattggt caaaacttgc
    13441 tctaattgag gtctgaaatc atctggcaga tgctgggcta ccaatccatt aacaatccta
    13501 tctatatctt ctaagtaaac aggaacgttt cttgcaaagg tcaaaacctg acgttgcaga
    13561 tttggaatgg cgactgccaa gccccaaatg ataaagagag cgatgataac aaagacaata
    13621 gtgatagcta taacacgatt aaccttatgc ttctccatcc aatcaacaat aggattcaac
    13681 aaataatata acaaaccaga caaaatgact ggcaacatca caactgctaa aaagtctaaa
    13741 acaggtgaaa atagaaaact aatcttactt aaaataaaaa gattcagtcc caataataag
    13801 gttactaaaa ataccgtaat tgccttgtta tctaaaaacc acttgaaaaa ccaagatagg
    13861 ctaaaatgtt tctctttttg ctccataaat acttcctttc tattgttctc tcattatacc
    13921 atttttgaaa caaaataact cttttttaaa gtgcttacat ctaattggat atctttaatt
    13981 tacttgtttt tgtgatataa tagtcttatc tttacacaga ggtatagtaa tgaaagaaac
    14041 tgtttatttt ggaacttata cacgtcgtac ttctcaaggg atttacaagg cagactttga
    14101 tacagaaact ggtcagcttt caaatctaga actttttgca gctgagccaa gtccaaccta
    14161 ccttgccttt gaccagcacc aacatttata cactgttggt agccaagacg ataagggggg
    14221 aattgcagcc tatcaaactg acgggactgt gttaaatcat gttgttgaag aaggagctcc
    14281 ccactgttat gttgctgtcg atgaaaagcg tgatttggtt tacgcagcta actatcacaa
    14341 gggacaagtc cttgtttata aacgccagga agatggtagt cttctactta gtgatatgga
    14401 tcaacacagt ggccaaggtc cacatgaaaa tcaagcttcc ccccatgttc actatacaga
    14461 tttaacacct gaccactatc tagtgacctg cgacttggga actgaccaag tcatcaccta
    14521 tgacctcgat caagaaggaa aattatctaa gctctatacc tatcacagca agccaggagc
    14581 aggctcacgc catatcattt tccataacca ctataaaatc gcttatctca tttgtgaact
    14641 caatagtact atcgaagttt taatctacga tggcgttggc gaatttgaac gtatgcaggt
    14701 catttcaact ttaccggaag cttacgaagg ctttaatgga accgctgcta ttcatctctc
    14761 taaagacggt aaatacctct acgcttctaa ccgtggccat gattctatcg cagtatatac
    14821 catccttgcg gacggtagct tagagttatt agaaatcgtt ccaacgcatg gtcagactcc
    14881 acgtgatttt gatttgacac ccgaccaaaa atttctcatt gttgtccatc aagactctga
    14941 caatgcaact gtctttaaac gtaattgtga caatggtcgt ctagcagaac tctccaacga
    15001 cttccatgtt cccgaagcag tctgcatccg ttttgctcct taaaacacca taaaaaatga
    15061 acttggtatt ttcaagttca ttttttctta tagtctattt ccgacattaa tacggttaat
    15121 ggcacgttgc aaagcaatct tagcacgacg ttcttggtca atcaaatgtt tgtcttgtgc
    15181 ttcttcaatt gcacgttctg cacgaagttt ggcacgttct gcacgactga tatcgatatc
    15241 acgagcacgt tctgcagagt cagcgacgat tgtaatcata tcattggcaa tttcaataac
    15301 gccaccgttt actgcaatcc agttcacgtg atctttatca tcgatacgtt ttacctttac
    15361 ttcatcaact gctaaaaccg caatcatatt ttcatgtcgt ggcaagatcc ccatctcacc
    15421 atccagagtt cgaaccgata catagctggc atggtgatca tagacgagac catctggtgt
    15481 cacgatctgg acagttaact gagccatagg tcacctctta aaatcccatt ttttcagctt
    15541 ttgcaatcac atcttcgata gaacctacac cacggaaggc atcttctggc aagtggtcgt
    15601 atttaccatc aaggatttcc ttaaagccac gtacagtttc agcaactgga acataagaac
    15661 ctggctgacc agtaaattgt tccgcaacat tgaagttttg tgacaagaag aactggatac
    15721 gacgggcgcg agcaaccaag gtcttttctt catcagaaag ctcatccata ccaaggatag
    15781 caatgatatc ttgcaattca tggtaacgtt gaaggacacg ttttacttca gcagcaactg
    15841 catagtgctc ttctccaacg atttcaggtg ccaaggcacg tgagcttgaa gcaagtgggt
    15901 caacggctgg gtagataccc aattgtacca atttacgttc caagtttgtt gttgaatcca
    15961 agtgagcgaa ggctgttgct ggcgctgggt cagtatagtc atccgctggc acatagatag
    16021 cctggataga ggttacagaa cccttcttgg ttgatgtgat acgttcttgc aattgaccca
    16081 tttccgtagc aagtgttggt tggtaaccaa cggctgatgg catacgaccc aaaagggcag
    16141 atacttctga accagcctga gtgaaacgga agatattatc gataaagaga agcacgtctt
    16201 ggccttccac atcacggaag tattcagcga ttgtcaaacc agtaagggca acacgcatac
    16261 gtgctcctgg tggctcattc atctgaccaa agaccatggc tgttttctcg ataacgcctg
    16321 attctttcat ttcccagtaa aggtcattcc cctcacgagt acgttcccca acaccagtaa
    16381 atactgaaat accaccgtgc tcttgggcaa tgttgtgaat caattcttgg attaagacgg
    16441 ttttaccaac tccggcacca ccgaaaagtc caactttacc acctttaagg taaggggcaa
    16501 gaaggtcaat aaccttgatc cctgtttcaa ggatttcaga agaggtagac aactcatcaa
    16561 aagttggagc ttttttatga attggctgac gctctgcgtc ttctgtaaaa ggagcttcca
    16621 aatcaatggt atctcccaaa acgttgaaga cacgtcccaa agtttcttta cctactggta
    16681 cagagattgg acgacctgtg tccaatactt ccattccacg agtcaaccca tctgttgatt
    16741 ccatggcgat agtacgaacc ataccatctc ctaactccaa ggctacttca aggacgattt
    16801 ttgtttttct ttcgtcattt ttgtagacga caagtgcatt gttaatctca ggaagttttt
    16861 cccctgctgc aaacaaaacg tctacaacgg gaccgataac ctgagcaatt ttacctgaac
    16921 tcatctcctt ctcctattct atataagata ctgtcggttc ctagctcaac taggtcctaa
    16981 gttcattttc atacgagctg gactagagcc tattctaagg cactagcacc tgctacgatt
    17041 tctgtaattt cttgtgtaat cgccgcctgt ctggcacggt tatactgaat tgtcaaatca
    17101 ttgatgactt tcttagcatt atctgtcgct gtttgcatgg ctgtcatacc cgcagcattc
    17161 tcagctgtct tggcatcgat aatggcaccg taaatcatac tttctgcaaa ctgaggcaac
    17221 aactgctcca gaatttcttc tcggctggtt tccaattcaa aagtcaagct gtactcttca
    17281 tccgcctcat ttggatccaa gtcaacaatc ggaagcattt gttccacacg catttgactg
    17341 gttagcgtat tgacatggtg gttgtagcaa acataaagct catcaaagag ttcattttgg
    17401 tacatttcaa cagtttttga aataatctta cgaacttgat caaagctagg ttggtctgcc
    17461 aagccacgta attcataaag tggttgaata ccgcgagcct taaagaaatc agctcccatc
    17521 ccaccgatac agatcatttc aaaaccttta ccgtctgggt ggtattcttc tttcaactcc
    17581 ataacagctt tcaaaataga ggaattataa cctccaacca aaccgcggtc tgaagtgata
    17641 acgatatagc ctgtcttctt cacagaacgg ctaatcaaca tcggattagt tgaagcacca
    17701 gctccattac catgaaggat atctgtcaaa agtttacgca ctttctgagc gtaaacttgg
    17761 aagttgcgag cagcttcttc agagcgacct aacttagcag ccgataccat ttgcatagca
    17821 ttagtgattt gactcgtatt ttttgttgag gcgatttttg ttttaatatc atttagagac
    17881 actgccatct gacacctcta ttcttattgg aagctagatt gattgagaaa ctctgtaatc
    17941 gcagcatcca agactgcttc ttctggcaag tcttttgtat cacgaatggt ttccaaaatc
    18001 tctggatgtt gagcatcaaa gaaggcatgg aactcttcct cgaaacgaac aatatcatct
    18061 actggaacag tatccaagaa accatgtgtc aaagcataaa gaatggttac ttgtttctca
    18121 acaggtaatg gtttgtgaac aggttgtttc aagacctcaa cggtacgacg tccacggttc
    18181 aacttagcct gtgttgctgc gtccaagtca gaaccaaact tagtaaaggc ttccaactca
    18241 cggtatgaag caaggtcgat acgaagtgta ccagcaacct tcttcatggc tttgatttgt
    18301 gcagaaccac ctacacgaga tacagatgaa cccgcatcga tggctggacg aatacctgca
    18361 ttgaagaggc catcgccaag gaagatttgt ccatcagtga tagaaatcac gttggttgcg
    18421 atataggctg agatatctcc tgcttgtgtc tcgataaatg gtagggctgt aattgatcca
    18481 ccaccaagtt catcagaaac tttagctgag cgctcaagca aacggctgtg gagatagaaa
    18541 acatcccctg ggaaggcttc acgacctgga ggacgacgaa gcaagagcga tagttcacga
    18601 taagctaccg cttgttttga tagatcatcg tatacaatca aaacatgctt accttgatac
    18661 ataaattctt ccgccatagc aaccccagta taaggagcta ggaagagcaa tggagatggt
    18721 tgtgaagcag aggctgtcac aacgattgtg tagtccaagg caccgtactg acgaagtgtt
    18781 tctacttgcg tacgaactgt tgattctttt tgtccaatcg cgacgtagat acagatcata
    18841 tcttgatctt tttggttcaa gattgtatca atcgcaatgg ttgttttccc tgtctgacgg
    18901 tcaccgataa tcaactcacg ttgaccacga ccaatcggta caagggcgtc aatagctttc
    18961 aaaccagttt gcaatggttc tgaaacagac ttacgttgca taacaccagg agctggtgct
    19021 tctactggac gagttttatc agtgtggatt tctccaagac cgtcaactgg acgaccaagc
    19081 ggatccacaa cacgaccaat cagactttca cctacaggga cttccatgat tttccctgta
    19141 cggcggattg tatcgccttc acggatatct gtaaagtcac ctaggatgat aataccaacg
    19201 tctgttgact ccaagttttg agccatacca taagagccgt tttcaaaatt caacaactct
    19261 ccactcatga cattttcaag gccatgagca cgcgcgatac cgtccccgat ataggttaca
    19321 acacctgttt cagtcacatc aaaattgggt ttgaaatttt caatttgttg cttaattaaa
    19381 gcgctgattt cttgtgcgtt aattgccaaa agaacaccac tttctatttc aaattttctt
    19441 taacaacttt aagttgttgt ttaatactca catcaattgt cttgtgattg gcaaaaatga
    19501 caaaaccacc aatgagactt tcatcgattt gttcttttac actccttact ttcagagaca
    19561 tttttttctc aatcaaaggg agcaaacgag tcttctgttc atcagttaga ggatgagcag
    19621 acgtaatcgt cacttcaaat cgatttgttt ctttttcaag tcggttcaag caatctacaa
    19681 gcacatcata aaaaagattt gctctgtgat tgtaggccag aacctggata aagttttgta
    19741 ataaaggcga cacagaatct tggaaaaaag caattgtttt ttccttatcc gactcgtcta
    19801 ctgccacttg ttttaaaaaa gaaggcagac ctgttttttc aacaacttgc ttgatttgag
    19861 tcaagtctga aaagatacgg tcttcttctc ctttttcaag taccaattgg acaaaaggca
    19921 tgctgtattt ttcaattacc tttactgttt tcttgtccat taagcttctc ctagctgatc
    19981 gatatactga tcaatgagtg ctttatgggc atgactgtca aggttttgtg agatgatttt
    20041 accagctaag ctgatggtca aatctgcgac ctcaccctta acactctgta aagcttctac
    20101 tttattttga gcaatttctt gattggcttt ttcttttaag tgtcctgctt ctagtttagc
    20161 atctgctaag atattagcct tactttgctc agctgtttcc tttgcatttt caatgattgt
    20221 cttagcttct ttacggctac cagccaattc atcttcgcgt ttttgagcca atacttctgc
    20281 tttttgacgg gcttcttcag ctctgtcaat atctgaagca attttttcag ctctttcttc
    20341 gaaaatgcct gtaatattag accatgcaaa ttttttaatc aagactagca aaagaataaa
    20401 agagccagtg attaaaacaa aattaccaat taattcacct actgttacgt gcatcagtta
    20461 ctcctttcta ttcctcttca atattaatct tattccctaa atacacagat gtcaaaagag
    20521 taaaaacata agcttggatg caggaaataa agacagaaaa tgcagtccaa gccaaattag
    20581 ctccaaaggc tactggatac caataaatag cttggtggga aagaagaagt aacaaactcg
    20641 tcatgacctc tcctgcaaag atattcccaa aaatccgcaa agccaaagat aagaagtttg
    20701 taaattcttc caagatattc atcggtatga caaaaacagg actcataaaa gattttatac
    20761 tttttttaaa tccacgacga cgaacgcttt ctatatgtgt caacaaaatg acaagaaaag
    20821 ataaggttaa gtcatactgt aaattagcgg ttggcgaact ccaccagtta gtcccatcga
    20881 tcgtttgaat ctttgtcatt aagccaaggt tattggcaat caccatgaaa aggaataaac
    20941 aaaggaaaaa gagtgagtaa tctttcatgt agcgcgaacc aatgttaggt tctgtaaatc
    21001 caataacaaa gtcatagaca tactcaagta cattttgctt tcctttgggt ttcaaggtca
    21061 tattgcggct tgcccaataa ataaagacaa aaataactcc cacaatcaac aaagtcatgg
    21121 ctaacatagt cagattgaag ataacaggac caatagagat gattggatta atactttctt
    21181 ccatctaaaa tcctcccttt tcttctcgtt ccatgtattt atttgataat aaatgagaag
    21241 acaagagtta caaagaaagt tccttcaata aaggcaacac ctaaaaacat caaactacga
    21301 aactcagaaa gcatatctgg ttggcgtgct actgatttaa acagtccatt catcaataaa
    21361 ccttcaccga cagatacgcc catacaggca atacataagc ctaaaaatgt taaattcata
    21421 acaaattctc ctttaaaata aaatttactt acttagtgta gtcctaaaat ttaaatttgt
    21481 caacttttaa ccgctattgg aagcgtttaa taaaaattat ttccattt
//
LOCUS       DAVMHG010000023        21110 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMHG010000023 DAVMHG010000000
VERSION     DAVMHG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.023"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            81..281
                     /locus_tag="VYR08_000671"
     CDS             81..281
                     /locus_tag="VYR08_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3466240.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKSDITIDELFYS"
     gene            296..872
                     /locus_tag="VYR08_000672"
                     /pseudo
     CDS             296..872
                     /locus_tag="VYR08_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1306..1758
                     /locus_tag="VYR08_000673"
     CDS             1306..1758
                     /locus_tag="VYR08_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466241.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            1755..1955
                     /locus_tag="VYR08_000674"
     CDS             1755..1955
                     /locus_tag="VYR08_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466242.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIKAKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKSYQARA"
     gene            1957..2172
                     /locus_tag="VYR08_000675"
     CDS             1957..2172
                     /locus_tag="VYR08_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466243.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSTQFERENQRTI
                     ETPSEDLNPDYGRYIQLAGVNVWGGTE"
     gene            2169..2510
                     /locus_tag="VYR08_000676"
     CDS             2169..2510
                     /locus_tag="VYR08_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV3466244.1"
                     /translation="MSLSDNDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRYGVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTILLNADLESY
                     KETLKEVGGYV"
     gene            2503..2778
                     /locus_tag="VYR08_000677"
     CDS             2503..2778
                     /locus_tag="VYR08_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV3466245.1"
                     /translation="MFSLSRESEQDLAQGILEVVERYLEARDRLKPRLTGLISAQEVM
                     DELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYRISDIWKFMGVENG"
     gene            2919..3785
                     /locus_tag="VYR08_000678"
     CDS             2919..3785
                     /locus_tag="VYR08_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV3466246.1"
                     /translation="MTIYETRGFQGNLVYPFDKIEPFQYIERFKPLVVPEGANIEEFK
                     RTQAPYCISGKVTPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKDVSSTLFGY
                     SYILYPTIKHNLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDLASYQKIVEHGLDYPVPQTTAEPVKQNATSLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            3829..5304
                     /locus_tag="VYR08_000679"
     CDS             3829..5304
                     /locus_tag="VYR08_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV3466247.1"
                     /translation="MEVREQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVT
                     EKVAMPQTFTVAKILSEIVTFTFISKINIPDYSLLYIYDLDEGIYTASNDLFNLLCKT
                     FDVRIKPREWPQIKLMVRTLVKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITS
                     KISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPNHTRNKFAI
                     FYGDGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYL
                     KNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVP
                     FNADFNGEKEKPWIKNEFLANDEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDN
                     DYLLSWVKHEYMEKGWHELDVVPVFILTRSLKHYAEDMGIPKPNVYGAGKETIRHLQQ
                     LTPNHYKLKRARVKVEDYDKLDPLEFERTKLSKTNHAVVKD"
     gene            5667..6197
                     /locus_tag="VYR08_000680"
     CDS             5667..6197
                     /locus_tag="VYR08_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466248.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTAMVAGHALLGYMTGTYEVPS
                     ISITHKDKGTLVAEYVEDHKLNKTFKRICDSFKDYYNQLVDDEAFEERYKRERVLQLK
                     ESEDFESLLEKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGDESISLIQKLD
                     VEGEYKGLAVYTEHLK"
     gene            7344..7763
                     /locus_tag="VYR08_000681"
     CDS             7344..7763
                     /locus_tag="VYR08_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV3466249.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEALEGGIIQKPTLSHSR
                     VQTSRENKTENNFISVLKLKEDTLQRIERITEERMEISGLIDKLANPLERSVLRLFYL
                     NDLDIWGVAEEIGKSKASIYRAKKAAIEHLACMVNGN"
     gene            8511..8720
                     /locus_tag="VYR08_000682"
     CDS             8511..8720
                     /locus_tag="VYR08_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466250.1"
                     /translation="MAKVKEKWNPTISHIVPKGTKLSDGTILDKETTLTQEEFTKNPP
                     VIPAGHPYYNLLARISREEIEKEDL"
     gene            9272..12103
                     /gene="uvrA"
                     /locus_tag="VYR08_000683"
     CDS             9272..12103
                     /gene="uvrA"
                     /locus_tag="VYR08_000683"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV3466251.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            12096..13157
                     /locus_tag="VYR08_000684"
     CDS             12096..13157
                     /locus_tag="VYR08_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV3466252.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            13289..13687
                     /gene="spx"
                     /locus_tag="VYR08_000685"
     CDS             13289..13687
                     /gene="spx"
                     /locus_tag="VYR08_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV3466253.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            13753..14322
                     /locus_tag="VYR08_000686"
     CDS             13753..14322
                     /locus_tag="VYR08_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3466254.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            14409..14675
                     /locus_tag="VYR08_000687"
     CDS             14409..14675
                     /locus_tag="VYR08_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV3466255.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            14679..15098
                     /gene="ruvX"
                     /locus_tag="VYR08_000688"
     CDS             14679..15098
                     /gene="ruvX"
                     /locus_tag="VYR08_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV3466256.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            15114..15419
                     /locus_tag="VYR08_000689"
     CDS             15114..15419
                     /locus_tag="VYR08_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV3466257.1"
                     /translation="MSHDHNHDHEERELITLVDEEGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            15831..17081
                     /locus_tag="VYR08_000690"
     CDS             15831..17081
                     /locus_tag="VYR08_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3466258.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLHPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            17078..17608
                     /locus_tag="VYR08_000691"
     CDS             17078..17608
                     /locus_tag="VYR08_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV3466259.1"
                     /translation="MTPLFELVILGKLLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTG
                     QDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIV
                     VDDMDRDPSDQIVLTK"
     gene            17754..19286
                     /gene="cls"
                     /locus_tag="VYR08_000692"
     CDS             17754..19286
                     /gene="cls"
                     /locus_tag="VYR08_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV3466260.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            19544..21082
                     /locus_tag="VYR08_000693"
     CDS             19544..21082
                     /locus_tag="VYR08_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV3466261.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQTKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDTIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
BASE COUNT         6770 a         3903 c         4709 g         5728 t
ORIGIN      
        1 gaaaaaatgt gcaaactcct ccgacctttc acgctttcaa tctattcatg gagggggatt
       61 ttttagaaag ggggaaagct atgagtaaac tacgaggcta tcgagttatg ttaggactaa
      121 ctcagcaaca aatggcggac aagctaaaaa tttctttgca gtcatacaac aacaaagaat
      181 taggtaaaac gccattcaat gacaaagaac gcctagcgat taagtcaatg gttgcagaaa
      241 tcaaatcaga tataaccata gatgaactat tttatagcta gaaaggagca aaccgatgga
      301 actagtctat atggacggta aaaaagagcc ttatacgctg agcagtattg tggcagaatg
      361 cacgggattg caacatcaca caataaccaa gacgatccgc aaacatcaag taaggtttga
      421 acagttcgga aaggttgaat ttaaaatcca agctatggaa agtggccaga ataccaagga
      481 ctatattttg aacgaacaac aagcgacctt gttagttaca ttcttaaaga acaccgagca
      541 agtggccaac ttcaaaacca acctggtcaa agctttcttt gaaatgcgtg aggaactttc
      601 taagtttcgt atgcagaggg cgctagaaaa gccaaaaaga aaaacactgc atgaacaacc
      661 tgctgctaaa agcagtaact gacatgaacg ctaagcagtt aagggaagaa cgtgggggct
      721 ataacggtat tgatagcttg accagtgacg agctggagca atatcaggca tttgaggata
      781 tggtaatagc catgattggc ttgaatatga gttatcagga aatcaaagct atggcattca
      841 gaaataaaaa aacacgccaa agaagcgcgt gagagcaaca aaaaaggctt accgagacca
      901 atcagcagag cctttcacac taacactaaa acgaatttaa caaagcaggc aagctattat
      961 caaagaggtt ttagtaaaga tttataccta gattatagca tatttagggc aatttgacca
     1021 tacggagagc gccaactctt taaactggta ctttctcacg ctttcaattt ggcgactctg
     1081 agcgtgagga ttagcgttat aagaaacaac tcaaaaaagc cccacgctca aagattggac
     1141 ccagagagcg tgaggctgta ggcaagaaaa actgtataag aaacaaccat tcaaaatccc
     1201 cacactcaaa ttttggtcga ggagagtgtg aggatatcca gtatagtaaa aggcattaaa
     1261 aagcccttag ggcaattaca caacatagaa acaaaggtga aaaacatgac agaaacaaca
     1321 tacgagaatt taactagaca tatagacaga attagcgcag aaatgcgaga aattggcgaa
     1381 aaaaatgggc ttagtagact ttcattattg gcaaatcaga caaaattcat caaagaaaat
     1441 ttacaccgtt tactttggat tgaacttcct gaattgaatg acagtcataa aatcgaggca
     1501 gtttctaaaa gcactacggg aatgtttttc cacgctggta tttttgaaat ggacgctatg
     1561 cgacaagcat tctttaaacg ccaagctatg cacttttttg acaacgaagc agagcaacag
     1621 gcgtatatag aacatgctga aaaggagtat ttagaggcta ctataacctt aaaagatatt
     1681 ctttttaact ctaaaaatgg aactcagaga gtaaataaaa gttgtcttat agagaagttt
     1741 gaggaggcaa tgcaatgaca ctagacctag acaacatgac acaagcagaa tttgatgaag
     1801 taatggctga aatcaaggcg aaacgcccaa acctcttcca gtttattgcg gattttgtag
     1861 atcggaaagt aagcactgag gaggtagacg aatacctgaa gatgaaacat gaaaaacaag
     1921 tggaatttat caagagttat caagcgaggg cataacatga atgagttaga tttgaccaat
     1981 acacaatcgg taatctttat ggtggtattg attggcttac tgctttattt aaaccaccga
     2041 gaccgcaaaa aaagcaccca atttgagcga gaaaaccaac ggacgataga aacacctagc
     2101 gaggatttaa accctgatta tgggcgttat atccagcttg caggggtcaa cgtatgggga
     2161 ggaactgaat gagtctatca gataatgaca aacgagtatt gagactaatc aaggtaggtg
     2221 ctgagaactc cataacaggg gcagaaatca gcctgatcac taagctggca gaaagaaccg
     2281 tgcaagatat tatcagccgt ctaatcacgc gctatggcgt tccaatcgtt ggggttcgac
     2341 acggaacatt tagaggatat tttatcccag ctaacaaaga ggagttactg gacggagcaa
     2401 aggcattcta taaccaagta caggaagaac agaaacgcct aactatcctt ttaaatgctg
     2461 atttagagag ttacaaagaa accttgaagg aggtgggcgg atatgtttag cctaagccga
     2521 gaaagcgagc aagacctagc gcaaggcatt ctggaggtag tggaaagata cctggaagcg
     2581 cgtgatagac tcaagccacg gctgacaggt ttaatctcag cccaggaagt catggatgag
     2641 ttagatatta agtatatgac gctccaaaaa tgggaaaaag cagggctaag gcgataccag
     2701 cccccactag aagatactag gaaaatctat tatagaattt cggacatctg gaagtttatg
     2761 ggggtggaga atggctagat ataacacaca cccagcacaa ggcggaggtc attatcatga
     2821 cattaaattg tataagcacc gtggcagatc tttggaacag ttccaagaac aaaagctact
     2881 gaagaagtta aaaaagaaac gcaaaaaggg gcgatagtat gacaatttac gagactagag
     2941 gctttcaggg taatcttgtc tatccatttg ataagattga gccgttccag tacattgaac
     3001 gctttaagcc tttggtagtc cctgaagggg caaacattga agaatttaaa cgtacacaag
     3061 cgccttactg tatcagtggg aaagtgacac cagaaaagaa tggcagttac aagcgaaaca
     3121 actccagtct aatctatcgg gatttgattt tccttgatta tgacgatata caggggacgt
     3181 ctgagggctt tataaaggac gtttctagca ccttgtttgg ctactcatat attttatacc
     3241 cgactattaa acataactta gaaaagcccc gttttcgcct tgtagtgaag cctgacaacg
     3301 tgatgaatga ggcaacctat aagcaggtag tgaaagagat tgctgacaag attggactac
     3361 cctttgatat ggccagcctt acatggtcac aacttcaagg gctaccagta acaacaggcg
     3421 acctagcaag ttatcaaaag attgtggagc atggacttga ttatccagta ccacagacaa
     3481 cggctgaacc agtcaaacag aatgctacaa gtcttccata cactccaagg ccaagcggtc
     3541 agaaatctat gaccatgagg atcattgaca cgcttttcaa cggctttgga gacgagggag
     3601 ggcgcaacgt agcattgaca cgctttgtag gcttactctt taataagtgg gttgattgtg
     3661 acctagagac cgcctatgaa cttacaaaga tagccaatag cgtgacggtt gaaccattgc
     3721 ctattgaaga gctagaccgc acttttagca gtatagcacg ggctgaatat agaaagagag
     3781 gttagaacca tagaactaga agaattagca aacctaaaaa gtgaaatcat ggaggtcaga
     3841 gagcaagaac accctcctag aacgatgaag gagcttgaaa atcgtatctt taaagctggt
     3901 gaagaatggc gggcagaaca taccgaaact aaagtaaatg agaccacagg ggacgtcacc
     3961 gaaaaggtgg ccatgcccca aacattcaca gtagctaaaa tactgagcga gattgtcaca
     4021 tttactttta tcagcaagat caatatacct gattatagcc tgctctatat ctacgattta
     4081 gacgagggta tctataccgc tagtaatgat ctattcaatc ttttgtgtaa gacctttgac
     4141 gttagaatta aacccagaga gtggccacag attaagttaa tggttagaac attggtaaag
     4201 atacgaaaac ctttagaaag tgctaacctc attcccgtac agaacggcat tatcaactta
     4261 gaaactaagg aactactccc ctttagtcct aagtatgtaa ttacaagtaa gataagcacg
     4321 gcctaccacg cgcctaaacg agtcccaaca gatagagagg gcaaaacttt tgatgattgg
     4381 ctaaactcaa tcgcttgtaa tgatagtgaa ctggtaacac tgttttggca gattatcctt
     4441 gaagctatta accctaacca tacccgaaat aagtttgcta tcttctacgg ggacggtaac
     4501 aacggtaagg gaacatttca gcgcttcctt atcaacctga taggggaaag caatatatca
     4561 gccttaaaac ctgcacagtt tgctgaaaag cataacttag aaaccctggt aggtaaagtt
     4621 tgtaatatcg gagatgaagc cccaaacgaa tacttaaaaa atccgtctga tttaatgagt
     4681 attactagtg gcgacactgt tctagtcaac cccaaaggtc gcccagcctt tgaagctact
     4741 ttcaagttgt tcaatatctt ttcagggaat tatatcccca atggtggcaa caagacaaaa
     4801 ggctggtatc gtagaattat gatcgtccct tttaatgctg actttaacgg ggagaaggaa
     4861 aaaccttgga taaaaaatga atttttggcc aatgatgaag tattagagta tgcactttac
     4921 aaagctatca atcaagagcc atttactcac tttatcgaac ctaaagcagt taaaggactc
     4981 ctagaagaat accaggagga taatgactac ttactttcat gggttaagca tgaatacatg
     5041 gaaaaaggtt ggcatgagct ggacgtagta cctgttttta tactaactag gtcgctgaaa
     5101 cattatgctg aagatatggg aatacccaag cccaatgttt atggggcagg aaaagaaacg
     5161 ataagacact tacaacagtt aacacccaat cattacaaac ttaaaagagc gagggtcaaa
     5221 gttgaagatt atgacaaact agatccgcta gagtttgaaa gaaccaaact atccaaaact
     5281 aatcacgctg tcgttaaaga ctaaaatgtt accttctttt tatgccctaa acccttggta
     5341 tatctaggtt tttacccaaa aatgttacct tcttgttacc ttgtttagaa agaaggtaac
     5401 gttgttaaac ccttgatact actgactttt ttgggaaaat gttaccttgt taccttgttt
     5461 ttaactctct atatagggaa aaatagtatt tttatatata agggctatga cttcaaaaga
     5521 aggtaacatg gtaacaaaag ggtctaaacc cttgggggtg taaggcttaa ctatgttacc
     5581 tagaaggtaa caaaatcact aaagaaggta acaaattaac gaatattaca taatttacct
     5641 taaactaaga aacggagaaa aacaacatga cattaaaaac attttcagat aaagccaaaa
     5701 catttacttt cacttacgaa tttaaagacc tagacactgc tatggtagca ggacacgcgc
     5761 tactgggata tatgactggg acttatgaag taccatctat ttcaataact cacaaggata
     5821 aagggacgct tgtagctgag tatgtggaag atcacaaact aaacaagacc ttcaagcgta
     5881 tttgtgacag ttttaaagac tactacaacc aactagtgga cgatgaagca tttgaagaac
     5941 gttataaacg ggagcgcgtg ctccaactca aagagtcaga ggactttgag agtttgctgg
     6001 agaaagtaac agactacgag ctggaactgt tagactatgc tgatcgcttg ctttctgata
     6061 agcctatccc tatggactca atgaccgctt ttggtacgtt ggaaatgctg ggcgatgaaa
     6121 gtattagcct aatccaaaag ttggacgtag agggcgaata taaaggcctt gctgtttata
     6181 cagagcattt gaagtagaaa acaggggagc aatccccttt tagtatgtaa cccaatcgat
     6241 ttaggttttt tacatgataa tttgtagtag tgtgcaattt acaaaacaaa caaatactac
     6301 atattagacg gtttgaatgt taaaaaagct agatatatca agggtttaag taagtagagc
     6361 gttccaaaaa agggtgcggt aatcgttcca aaaaaagggt gaggtaaggg tgaggttaca
     6421 tatatataaa gtacaaggtt agtacaaggt tgtgtattta aaaatattaa aaataggcta
     6481 agattaggct aatgtcacat acttcaaaat actaaggttt tagcaaggta cagggctttt
     6541 tttctagtga tatgacccta agattaccct aaggtatagc tactttttta gaaccataga
     6601 acggtaaggt tttggtaagg tatcgaagtt ttccaatggt gaggttttgg tgaggtaaga
     6661 gcaaaaaatg taaaggtttt gtaaaggttg cgtatttgaa agccccaagg ttagcacaag
     6721 gtagagaggt ttaccgaggg ttagaaaagg tgagattttg cttaggtagt ggcagtaaat
     6781 atgcctgaca aatggcaggg ttatgtgtgt tgaactccga gcgaacgccg agaaagtgga
     6841 tatatcccct atcatttatc aagcagataa gagaaataat aaccctcctt aactcccgga
     6901 gaaacgacaa gcagataagc ggagttttta cccctcttat ttccgattta ctccttgcag
     6961 aatgataggc agataaggac gacaataagc cttcttaatt gcttacaaaa cgatagggag
     7021 ttaagacgaa tttaaaagct ctttattttc gtttttctcc ttacaaaatg acagggaaat
     7081 aagaactaaa ataactctcc ttattttcct accaaatgat aaggagataa gagcaattta
     7141 aaagtctgtt attttggatt tgttgcttac caaatgataa gcagatcagc aaaggaataa
     7201 cttgccttaa ttgcttatca aatggtacgt agaatggact tgaacctaga gcgaagctag
     7261 agaaaatgaa aaaaagccag cacacggctg actcctttat gaattattcc ctaaaactat
     7321 tatatcacaa aggaggccga ggcatgacac ccgagcaggt aaaagaaaaa ctagagggcg
     7381 ttaagtggat aaataaggag atcaaaggct tatatttgga attggaagcc ctggaaggtg
     7441 gcattatcca aaagccaaca ctaagccata gcagggtgca gacaagcaga gagaacaaga
     7501 cagagaacaa ttttataagt gttctgaagc taaaagagga tacgctccag aggattgagc
     7561 gcattactga ggaaagaatg gaaatatctg ggctgatcga taagctagcc aatccgcttg
     7621 agcgctctgt tctgagactt ttttacttga atgatctcga tatttgggga gttgctgagg
     7681 aaataggtaa atctaaagct tcgatatatc gggcaaagaa ggcagctata gaacacttgg
     7741 catgtatggt aaatgggaat tgatttaacc ttaccacaga gcctagtgcg tatagggggc
     7801 actaggcttt atattcgccc cttattcgtt ttgttttgga ctggataact aaatcaccac
     7861 ggataaacta caagccctca gaaacaaatc taggggcttt tgtgagataa aaaaataaag
     7921 gtgacccacc gcctccacag caagctgctt catgggcgtt agaccacctt taccttaact
     7981 taattataaa tcacaatgcg gagtttttca agaaaggata agagactaga aaactagcta
     8041 cttattggaa caacaaagct agtgagctat tgaagaacgg taagattttg gttagataca
     8101 gagaaacagg cctaagcctg ctctaaatct aacttaattt cttcacaaac tattaaattc
     8161 aaataagctg agaaaacata aagggtaaca aaaaggggac ttaaattgaa gagttcagta
     8221 agcaagaaac taacacaatc aaacgtgctt tttttgtaaa aatgttgtat acttaagtaa
     8281 ataaaggaac tggtttggca cccaatggct tattttaggt cggcgcctaa gtcagttcct
     8341 ttttactgtt tcgaccaccc caaaggatat gcctctaaca ttattgcgtc aattagttat
     8401 ttaggctata attaaattaa cgaatagagg ttttccatct ccccttgaaa cagttcattc
     8461 cgcggtagga ggtgggacgc ctctattttt accataaagg agtatttaac atggcaaaag
     8521 ttaaagaaaa atggaaccca acaatatccc atattgtccc taaaggtacc aaactatcag
     8581 acggtacaat attagacaaa gaaaccacct taacacaaga agaatttact aaaaatcccc
     8641 ctgttattcc agcaggacac ccttattata atcttttagc tagaataagc agagaggaaa
     8701 tagaaaagga agatctatag tacaaagaaa tagtaaaata taatagtttt gcgcgtgatg
     8761 ttcgcttgaa aaggacttgt taaggtcaac aaaatcgata accacgcgct tttatctatc
     8821 tcaccttttc gatagataaa acaaccaata cgcgtgatga acatggtata atatattcac
     8881 agctggaggc ttgcacagtc tagccgatta gggagagtta gaccgagatg tcacacactc
     8941 ccttttttgg tataataaac ctatcagata agcaaaaaca actaaaaaag cgtagctgat
     9001 gactacgcta gtttcttgcc tgctgaactc gtcaaatctt agcccttttg tggggctttt
     9061 ttgctcccct ttttgcgtac tttgggggag caagtagtaa aaagtaatat tagtgttttt
     9121 tgttaaaaag cgcgtgatat aagggttagg aagccctaag gaaacataaa ataagaagtt
     9181 ttttattata ctacaaaatc ggccttttgg gtaatagaat ctcacttttt ttggtataat
     9241 ggtaagcaat aatggactag aaagaacaaa gatgcaagat aaaattgtca ttcatggggc
     9301 gcgtgcccat aatttaaaaa atattgatgt ggagattccg cgagacaagt tggttgtcgt
     9361 gactggcttg tcaggttcag ggaagtccag tctggctttt gataccctct atgcggaggg
     9421 acaacgtcgc tatgtagaga gtttgtccgc ctacgctcgt cagttcttgg gaaatatgga
     9481 aaaaccagat gtagatgcca ttgatggtct cagcccagct atttccatcg accagaaaac
     9541 gactagtaaa aatcctcgct cgacggtggg aacgacgact gaaatcaatg actatctgcg
     9601 tctcctctac gcacgtgtgg ggacgcctta ctgtatcaac ggacatggag ctatcaatgc
     9661 ttcttctgtg gagcaaatcg ttgataaggt tttggagtta cctgaacgcc agcgcttgca
     9721 gatcttggct cctgtcatcc gcaagaaaaa aggccaacat aagaatgtta tcgagaaggt
     9781 tcagaaagac ggatatgttc gtgtccgtgt ggatggggaa gtctatgatg tgaccgaagt
     9841 gccagagttg tctaagagca agcaacacaa tatcgatgtc gtggttgacc gtattgttat
     9901 caaggagggc attcgtagcc gtctctttga ttccattgaa gctgcccttc gtatcgcaga
     9961 aggttatgtg attatcgaca ccatggacga ttcggagttg ctcttctctg agcattatgc
    10021 ctgtccagtt tgtggattta ctgtcccaga gttagagcct cgtctcttct ctttcaatgc
    10081 tccctttggc tcttgtagtg agtgtgacgg cttggggatt aagctggagg tggatactga
    10141 tttggtagtg ccagatgcca gcaaaacctt acgtgaagga gcgctagcac cttggaatcc
    10201 tatctcatcc aactactatc caaacatgtt agagcaggcc atgaaagtct ttggagtggc
    10261 tatggataag ccttttgagg acctgtcaga agaagataag aacttgattc tctatgggtc
    10321 agatggtaag gaattccatt tccactatga gaatgaattt ggtggcgtgc gcgatatcga
    10381 cattccgttt gagggagtcg tcactaatat caaacgtcgt taccatgaaa ccaatagcga
    10441 ttacactcgc actcagatgc gtctctacat gaatgagctg acctgcggaa cctgtcaagg
    10501 ctatcgtctc aatgaccagg ccttgtctgt ccgtgtgggc ggccagcaag ggccacatat
    10561 tggagaaatc tcagacctgt ctatcgctga ccacttggac ttggtgagcc agttaacttt
    10621 gtctgaaaat gaagccatca ttgctcgtcc cattctcaag gaaatcaagg atcgtttgac
    10681 cttccttaat aatgtgggtc ttaactatct aaccctgtca cgttcagcag gaaccctttc
    10741 aggtggggaa agtcagcgca ttcgtttggc aacccagatt ggttccaacc tatcaggcgt
    10801 cctctatatc ctagacgagc cgtcaatcgg tcttcaccag agggacaatg accgcctgat
    10861 tgccagtctg aaaaagatgc gtgacttggg caatactctt atcgtggtgg aacacgacga
    10921 agataccatg cgtgaagctg attatctgat tgacgttggt cctggtgccg gtgtttttgg
    10981 tggtgagatt gttgcagcag gtacacctaa gcaagtggct cgcaacagca agtctatcac
    11041 aggtcagtac ttgtcaggca aacgtgtcat cccagtacca gaagagcgcc gtgtcggaaa
    11101 tggtcgtttt attgaagtga taggagcgcg tgagaacaac ttgcaaaatg tcacggctcg
    11161 ctttccacta ggaaaattta tcgcagtgac aggtgtgtcg ggttcaggga aatcgactct
    11221 aatcaacagc attctcaaaa aagccattgc ccagaagctc aaccgtaatt cagacaaacc
    11281 tggtaagttt aagactatta cagggattga gcatgtcgac cgtttgattg atattgacca
    11341 gagccctatc ggacggacgc cgaggtctaa cccagctacc tatacaggag tttttgacga
    11401 tatacgtgac ctttttgctc agacaaatga agccaagatt cgaggctaca agaaggggcg
    11461 cttcagtttc aacgtcaagg gaggtcgctg tgaagcttgc tcaggtgacg ggattatcaa
    11521 gattgagatg cacttcttgc cagatgttta tgtggcttgt gaagtttgcc acggaacccg
    11581 ctataacagt gaaaccctag aagttcacta caaggaaaag aatatttcgc aggtcttgga
    11641 tatgacggtc aacgatgcgg tagaattttt ccaacacatt ccgaaaattc aacgcaaact
    11701 ccagaccatc aaggatgtag gtctaggcta tgtgaccttg ggacagccag ctaccaccct
    11761 ttctggggga gaagcccaac gtatgaagtt agctagcgaa ctccacaaac gctcgacagg
    11821 aaaatctttc tacattctgg atgagccgac gacagggctt catacagagg acattgctcg
    11881 cctgctcaag gttttagctc gctttgtaga cgccggcaat acagtcctcg tcatcgagca
    11941 caatctggat gttatcaaga cggcagacca tatcattgac ttgggacctg agggcggtgt
    12001 cggtggtgga accatcatcg taacaggaac tccagaagaa gtagctgcca acgaagccag
    12061 ctatacagga cactatttga aaggaaagtt acatcatgaa taaacgcgta caagcatttc
    12121 tagctaaaat gcaagaaaaa gaactagatg gtatcatcat caacaatctt aaaaacgtct
    12181 attatttgac tggtttttgg ggctcaaacg gaacagtctt tatcagtcgt gaccgtcagg
    12241 tcttagtgac agactctcgc tatatcatcg cagctaagca agaaaccagt ggttttgaga
    12301 ttgtggctga tcgtgatgaa ttggctgtca ttgcaggaat tgttaaggac atgggcttga
    12361 ctcgtatcgg ttttgaagat gagatttcag tgtcttatta ccaccgtatg caggcagctt
    12421 ttgcaggttt gaacttgctt ccacaaactc agtttgtgga aggtcttcgt atgattaagg
    12481 atgaggcaga gattgcagcg attcgcaagg cttgttctat ctcagaccaa gctttccgcg
    12541 atgcgcttga ctttatcaaa ccaggaaaaa ctgaaattga gattgccaac ttccttgact
    12601 tccgcatgcg tgagttggga gcatctggct tatcttttga tacgatccta gctagcggta
    12661 tcaattcttc taaaccccat gcccatccaa tgcacaaacc agtggagttg ggagaagcca
    12721 ttaccatgga cttcggctgt ctctatgacc actatgtcag tgatatgacc cggactatct
    12781 atctagggca tgttagcgat gagcaggcag agatttacaa tacggttcta aaagctaacc
    12841 aagccttgat tgaccaagct aaggcaggct taggtttccg tgactttgac aaaatccctc
    12901 gtgatattat cattgaggca ggttatggcg actactttac tcacggcatt ggccacggta
    12961 ttggtctgga tatccatgag gaaccctact ttagccagac ttctacagaa actattaaga
    13021 caggtatggc cttgaccgat gaaccaggta tctatatcga aggcaaatat ggcgttcgta
    13081 tcgaggatga tatcctgatt acagagacag gttgtgaatt attgacccta gctccaaaag
    13141 agttgatagt catttagttg tttgtcaaga aaaaagtacg aaaatgacga aaaaagtcaa
    13201 aaaaaattaa aaataggtcg caagtcggat gttttttatg gtataataga ctaaactgat
    13261 agtaacatgt agcgaaaggg gtaggtacat gattaaaatt tatacagtct caagttgtac
    13321 tagctgtaaa aaagcaaaaa cctggctcaa tgcccaccag ttaagttata aagaacaaaa
    13381 ccttggtaaa gaaggaatta cgagagaaga attactggat attctaacca aaacagataa
    13441 cggaatagcc agcatcgttt cgtctaaaaa tcgctatgcc aaagcccttg gagtggatat
    13501 tgaagatttg agtgtcaatg aagttctcaa tctgattatg gaaacaccga gaattttaaa
    13561 gagcccaatc cttgtagatg aaaaacgcct gcaagttggc tacaaggaag acgatattcg
    13621 tgccttccta ccacgctctg tccgtaatgt agaaaatgca gaagcacgtt tgcgtgcagc
    13681 tctataaaca tcaaggctgg gagcacttcc cagtcttatt ctattttaat ttcaaaaaga
    13741 aagaagaaag aaatgaaaaa aatagttctt gttagtctag ctttcctttt tgtcctggtt
    13801 ggttgcggac agaaaaaaga aactggacca gctacaaaaa cagaaaaaga tacgcttcag
    13861 tcggcattgc cagttattga aaatgctgag aagaatacag ttgtaactaa gactttggtc
    13921 ttgcccaagt cagatgatgg tagccagcag acacaaacaa ttacttacaa agacaagact
    13981 tttttgagtc tagctatcca acaaaaacgt ccagtctctg atgagttgaa gacttatatt
    14041 gaccaacatg gagtggagga aactcaaaaa gctcttcttg aagcggagga gaaggataag
    14101 tctatcattg aagctcgtaa attggcaggt ttcaaacttg aaacaaaact attgagcgca
    14161 acggaacttc aaacaacgac tagttttgat tttcaagttc tggatgtcaa gaaggcttcc
    14221 cagttggaac atctgaagaa tattggtttg gaaaatcttt tgaaaaatga accaagcaaa
    14281 tatatttcag atagattggc aaatggcgcg acagaacaat agaaaatcca aataaataga
    14341 ctgcctgaat tgtagaaggt aaggaattat gctataatgg tactattcta aggaaagagg
    14401 gtgttagaat gggatttact gaagaaacag tacgttttaa attggacgat tccaataaaa
    14461 aagaaattag cgaaactttg acagatgttt atgcttcgtt gaacgataag ggttacaacc
    14521 caattaacca aatcgtaggt tacgtattga gtggagaccc tgcctacgtt cctcgttata
    14581 ataatgcacg aaatcaaatc cgtaagtatg agcgtgatga aatcgttgag gaattggttc
    14641 gctactacct caaaggacaa ggagtcgatc tataacctat gagaattatg ggattggacg
    14701 tcggttcaaa aacggtaggg gtggcgatta gcgatccgct tggttttaca gctcaagggc
    14761 ttgaaatcat ccagataaat gaagaacaag gcaaatttgg ttttgaccgc gttaaggaat
    14821 tggttgatac ttacaaggtg gaacgatttg tagtgggctt gcctaaaaac atgaacaata
    14881 caagtggacc gcgcgtagaa gctagtcaag cctacggagc aaagctagaa gagttttttg
    14941 gtttaccagt agactatcag gatgaacgct tgacaacagt ggctgctgag cgcatgttga
    15001 ttgaacaagc agatatcagt cgcaataagc gcaagaaagt cattgataag ttagcagctc
    15061 agctgatttt acaaaattat ttagatagaa aattttaata taaaggagag gctatgtcac
    15121 acgatcataa ccacgaccac gaagaacgtg aactaatcac actagtagat gaggaaggaa
    15181 atgaaacctt gtttgaaatc cttttgacaa ttgatggaaa agaagaattt ggtaaaaact
    15241 atgttcttct agtaccagtt aacgcagaag aagacgaaga cggacaagtt gaaatccaag
    15301 cttactcatt catcgaaaac gaagatggaa cagaaggcga attgcaacca atcccagaag
    15361 actcagaaga cgaatggaac atgattgaag aagtcttcaa cagctttatg gaggagtgat
    15421 acagtctggg agactgtatc agcccaactc ccagaaatgg ggaagagttg gaacgtccag
    15481 tggatgtttt tagcccagtt cctttaaata agagagaact ggtaggttcg gggggacgtt
    15541 tttagcccag ctcctttaaa taagatagag ctggtaagtc tgggagactg tatcagccca
    15601 actccagaaa tggggaagag ttggaacgtc cagtgaacat ttttacccca actcttagaa
    15661 attgagaaga gttggaacat ctagtggatg tttttagccc atattcacat tcatagtagc
    15721 tagttattag ctaaccaggt aagaggtcgg gacgaaaatc ctggccttgt ttttattagt
    15781 aaccatactt taatgcgata attgattgga cttttgtcaa ggagatgtgt atgtttgaag
    15841 tagaagaatg gctccatagt cggattggtt tgaattttcg atcaggtttg ggccgaatgc
    15901 agcaagcggt ggatttgtta ggaaatcctg agcagtctta ccctattatc cacgtaacag
    15961 ggactaatgg gaaaggatct accattgctt ttatgaggga attatttatg gggcatggca
    16021 aaaaagttgc gacctttacc tcccctcata tcgtctctat caatgaccga atctgcatta
    16081 atgggcaacc tatagcagac gcagacttta tccgtttggc tgatcaggtc aaggagatgg
    16141 agaaaacgct tctgcaaact cctgaccagt tgtccttttt tgaattgctg accttggttg
    16201 cttttcttta ttttagggag caagaggtgg atttggtttt attagaagtg ggaattggtg
    16261 gcttacttga cacgaccaat gtggtaactg gagagcttgc tgtcatcacc tccattgggc
    16321 ttgaccatca agaaaccttg ggtgatagtc tagaagcaat tgcagagcag aaagctggta
    16381 ttttcaaggc tggtaagaag gcagtgattg cgaaattgca tccagaagct aggcttgtct
    16441 gtcagaaaaa agccgaatct ttagctgtta acctttatca ggcaggtcaa gattttttaa
    16501 tgctgaatgg tgatttttca agctctttac taaatctttc acagctgaac ataggcttag
    16561 aaggagtcta tcagcaggag aatgcagcct tggcgttgca aacttttctt ctttttatgg
    16621 gagaaggaaa ggaagctgtt gatgaacagg ctgtaagaaa ggccttggaa cagacccatt
    16681 gggctggtcg cttggagcgt attcgcccac agatttactt ggatggtgct cataacctcc
    16741 ctgccttgac tcgcttggtt gagtttatta aagaaaaaga gcaggaaggt tatcgtcctc
    16801 aaatcctctt tggatccttg aaacggaagg attatcaagg gatgttgggt tatctgactg
    16861 aaaatttacc tcaagtggaa ctcaagatga ctggttttga ctatcaaggg tctttggacg
    16921 aaagggatgt gacaggttac gatatagttt cttcttaccg agaatttatc agcgattttg
    16981 aagaaagagc agatgctcag gatttgctgt tcgttacagg gtctctctat tttatctcag
    17041 aagtacgggg ctacctgctg gaccgtgagc agataaattg acccccttgt ttgaacttgt
    17101 tatactaggg aaattgctgg ttagagaaaa tttctctaaa ttggtagcag aaaggaaatt
    17161 catcatgaaa ttaaaaagat tcacactttc tcttgcttct ctagcaagtt ttagtctctt
    17221 agtagcttgt tcacaaagag ctcaacaggt tcaacagcct gctcaacaac ctgctcaaca
    17281 gaataccaat actgcaaatg caggaggtaa ccaaaatcaa gcggctccag tacaaaacca
    17341 acctgttgct caaccgaccg atattgatgg gacttatact ggtcaggatg acggagaccg
    17401 tatcacttta gtggtaactg gaacgactgg tacatggact gagctcgaat ctgacgggga
    17461 tcagaaagtc aaacaggtta catttgattc agcaaatcaa cgcatgatta ttggcgatga
    17521 tgtcaaaatt tacactgtaa acggtaatca aatcgtcgta gatgatatgg atagagaccc
    17581 atcggaccaa atcgttttaa ctaaataaga ctatgcaagt agaaagggct ggatttttca
    17641 gcctttttac ttttacctag aaaataatca taaatacttg ccttctaccg aatacctgcg
    17701 ttatactagt atcaattact tgtttttagc attcaaacta tcaaggaggg gatatgaaat
    17761 ataggaaatt tcaattattg atgtccaagt atggctttag tctttcgatt atgttacttg
    17821 aactttgtct tgtttttggt ctctttcttt atttaggacg catggctccc attttatgga
    17881 ttactgtcct cattctactg agtatcatca caatcatttc gatagtcaac cgtaatacga
    17941 ctcctgagaa taaggtaacc tggttgttag tagcctttgt gccagtattt ggtcccttgc
    18001 tctatctgat gtttggtgaa aggcgattgt ccaaaaaaga aatcaaacaa ctgaagaagc
    18061 taggctctat gcatttccaa gaagcaaata gccagctact aaaagagaaa ttaaaagaaa
    18121 gtgacaaggc agcttatgga gtcatcaagt ccttattgag tatggatacc aatgctgaca
    18181 tctatgatca aactgcctct acattttttc ctaacggaga agctatgtgg aaaaagatgg
    18241 tagaagatct taaaaaggct gagaaattta ttttcttgga atattacatt atagaagaag
    18301 gtttgatgtg gaatcgcata ctagatatac tagagcaaaa ggtagctcag ggtgtagagg
    18361 ttaagatgct ctatgatgat atcggctgta tggctacttt aacaggagat tatgcacatc
    18421 gacttcgtca gctgggcatc gaggcccata aattcaataa agttattcct cgtttgacag
    18481 tggcttataa taacagagat catagaaaaa tattgattgt tgatggtcag atagcctata
    18541 ctggtggggt caatctggca gatgagtaca ttaaccacgt cgagagattt ggttattgga
    18601 aggatagtgg aattcgctta gacggactag cagtaaaagc cctgacacgc ttatttttga
    18661 ccacttggta cattaatcga ggagaaatta gtgattttga tcaatatcat ttagaaaatc
    18721 attctatccc gagtgacggt ttaaccattc catacggaag tggacccaag ccaatttttc
    18781 gagcgcaggt agggaaaaaa gtttatcaga gtttaatcaa tcaagcaacg gaatcggtct
    18841 atattacgac accttatttg attatagatt atgatttaac agagacaatc aaaaatgcag
    18901 ctatgagagg ggtcgatgtt cgaattatta ccccttacat accagataag aagttcattc
    18961 agttagtcac gagaggagct tatcccgacc ttctttctgc tggggttcgg atttatgagt
    19021 atagtccagg ttttattcat agtaagcaga tgttggtaga cgaagatttt gcggtggtgg
    19081 ggacaatcaa tctcgactac cggagcttgg tacaccatta tgaaaatgca gtcttactct
    19141 ataaaactcc ttctataagg gaaatcgccc gagattttcg aaatatattt gcagattctc
    19201 aggaagtcta tcctcattct atcaaaacga gctggtatca aaagcttgta aaagaaatcg
    19261 cccagctatt cgcccctatc ttataagaaa tctagaactt aggatacaaa ccagacctat
    19321 ttttcttgtc ttttacgaat agaaagaaag aggaggggaa ttcatgctaa ctaaggaaga
    19381 agtgaatgat atgattgagt tcaaacagac acatttacac cgctttcggg aaattgaaac
    19441 ctttgtgaaa ctatgcaaaa aatcactcaa acagccatcg caagctgaca atcctaggac
    19501 tttttgatat actaagacag ataaattgaa caggagaaac gatatgagtg tgtatggtag
    19561 agtagaagaa gttcataagg agaatcgtga acctctagaa taccaaatcg aacaagaatc
    19621 gcatcatcgt gaatcaagtc gtcttccttt ggtgaaaatt ttactatgga gtacgcttgt
    19681 aacggggata actctaggag taccgctatt actcgactta atgagtgcac aagaagtgca
    19741 ggatttttat gcaggttggg cgcttcatca gacagggaag atttacagcg actattatgg
    19801 aagtcaaggt ttgctttatt atttgctgac ttacgtgagt cagggaggat ttttctttgc
    19861 catctttgag tggttagcct tggtagcagg aggatttttc ctttttagat cagcggacac
    19921 cttgacagag caaggagacc aagctggaca tctggtgact attttttaca tgctagttac
    19981 aggtcttgct tttggtggag gctatgcgac tcttttagcg cttcctttct tattcgcagc
    20041 ctttagttta gttgcggctt acctaagcaa tccaagccat gataagggat ttgtacggat
    20101 tgggctagct ttggcaggcg gatttttctt tgctccctta tcatcgctcc tgtttattgc
    20161 tgtagtgagt ttaggcttgt tggtctttaa ccttgggcat agacgctttg cgcatgggtt
    20221 ttatcagttt cttgcagtgg ctttaggttt ttcacttgtc ttttatccaa ctgcctacta
    20281 tagtgctgca acaggaagtt ttggggatgc gattagtggt attcgttatc ctattgacag
    20341 tattcgcttt gattttactt ctaaaatttt agagaatatg tttttttatg gcttgttgtc
    20401 ccttggtttg ggatttgtgg tttgtatctt tttaggtctc tttcaaacca aaccctttaa
    20461 actatacgtc atttcggttc ctgcaagtct tgtggtaatt ttaggtttga ttttactttt
    20521 cttttcacaa gagcctctgc acgcttctta tttgatggtc gtcttccctg ttttcctact
    20581 tttattggta accaatatta agagtcaaca gagggggcgt agtgctagaa gaagccgaag
    20641 agaaacgcca gttagcctat ggagtcgttt cttcaaagga aatctatatc tgctagtttt
    20701 tgggtttgtc tatcttttgt ctgttccttt tttgatgaag tttgtccttt atccagtacc
    20761 ttatcaagaa cgtaatcgtc ttgctgattt ggtaaaagag gagacaaata cggaagatac
    20821 tatctatgca tgggatgata ctgcgactct ttatcgtaag agtgagcgct tgtcgccatc
    20881 ggcgattttg tccccgttgc actatacagc aactgaggaa aatcgtaata agttacttaa
    20941 tgacttgaaa gaaaaacaac ctaaggtgat tgtggtaaat gacaaggtgg tagtctggtc
    21001 tgaagtggaa acactcttaa aagaaaatta ccaacaagta aagactgatt actcagagtt
    21061 taaagtctat aaaattaaat aaccaaatca atatcttgtg tatttttaaa
//
LOCUS       DAVMHG010000024        20931 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMHG010000024 DAVMHG010000000
VERSION     DAVMHG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.024"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            76..2298
                     /gene="recJ"
                     /locus_tag="VYR08_000694"
     CDS             76..2298
                     /gene="recJ"
                     /locus_tag="VYR08_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV3466262.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2473..4134
                     /locus_tag="VYR08_000695"
     CDS             2473..4134
                     /locus_tag="VYR08_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3466263.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4203..4982
                     /locus_tag="VYR08_000696"
     CDS             4203..4982
                     /locus_tag="VYR08_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV3466264.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5094..6314
                     /gene="murM"
                     /locus_tag="VYR08_000697"
     CDS             5094..6314
                     /gene="murM"
                     /locus_tag="VYR08_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV3466265.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVDQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6319..7551
                     /gene="murN"
                     /locus_tag="VYR08_000698"
     CDS             6319..7551
                     /gene="murN"
                     /locus_tag="VYR08_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV3466266.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7630..8331
                     /locus_tag="VYR08_000699"
     CDS             7630..8331
                     /locus_tag="VYR08_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV3466267.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8371..10218
                     /gene="uvrC"
                     /locus_tag="VYR08_000700"
     CDS             8371..10218
                     /gene="uvrC"
                     /locus_tag="VYR08_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV3466268.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10208..11041
                     /locus_tag="VYR08_000701"
                     /pseudo
     CDS             10208..11041
                     /locus_tag="VYR08_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11225..12025)
                     /locus_tag="VYR08_000702"
     CDS             complement(11225..12025)
                     /locus_tag="VYR08_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466269.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12196..12801
                     /locus_tag="VYR08_000703"
     CDS             12196..12801
                     /locus_tag="VYR08_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV3466270.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12814..14214
                     /gene="pepV"
                     /locus_tag="VYR08_000704"
     CDS             12814..14214
                     /gene="pepV"
                     /locus_tag="VYR08_000704"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV3466271.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14268..14846
                     /locus_tag="VYR08_000705"
     CDS             14268..14846
                     /locus_tag="VYR08_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV3466272.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15006..15158
                     /locus_tag="VYR08_000706"
     CDS             15006..15158
                     /locus_tag="VYR08_000706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3466273.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15201..15293
                     /locus_tag="VYR08_000707"
     CDS             15201..15293
                     /locus_tag="VYR08_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV3466274.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15464..16789
                     /gene="brnQ"
                     /locus_tag="VYR08_000708"
     CDS             15464..16789
                     /gene="brnQ"
                     /locus_tag="VYR08_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV3466275.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16887..17924
                     /locus_tag="VYR08_000709"
     CDS             16887..17924
                     /locus_tag="VYR08_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV3466276.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17926..18387
                     /locus_tag="VYR08_000710"
     CDS             17926..18387
                     /locus_tag="VYR08_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3466277.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18405..19121
                     /locus_tag="VYR08_000711"
     CDS             18405..19121
                     /locus_tag="VYR08_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV3466278.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19244..19669
                     /gene="rplK"
                     /locus_tag="VYR08_000712"
     CDS             19244..19669
                     /gene="rplK"
                     /locus_tag="VYR08_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV3466279.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19877..20566
                     /gene="rplA"
                     /locus_tag="VYR08_000713"
     CDS             19877..20566
                     /gene="rplA"
                     /locus_tag="VYR08_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV3466280.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6283 a         3862 c         4859 g         5927 t
ORIGIN      
        1 gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga attcttagaa
       61 agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct gcaggtagaa
      121 gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt ggctcggtta
      181 ttgtttgaga gagggattca gaaccaagaa agtctgaaga agtttttaga accttccttg
      241 gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga gcggattcgt
      301 caggctattg aagaagggga aaatattctt gtttatggag actatgatgc ggatggcatg
      361 acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg ccgagtttac
      421 ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa atactttatc
      481 gagcaagaag ggatttcctt gattgtgacg gtggacaatg gggttgctgg tcatgaggct
      541 attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca ttccatgcct
      601 gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc ggattatcct
      661 tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct gttagaagaa
      721 gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat ggtgagtctg
      781 acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca tacccagcgc
      841 attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt aacagaagaa
      901 acggttggtt tccagattgc tcctcgtttg aatgccttgg gtcgcttgga tgatcccaat
      961 cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat tgcccttatg
     1021 attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga agaagccaag
     1081 accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg gaatcctggg
     1141 gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt cattgttctt
     1201 aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt cgatattttt
     1261 gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc aggtgcagcg
     1321 ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga agattatgtt
     1381 cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga agagttggat
     1441 ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc ttttggaatg
     1501 gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc tcgtactatg
     1561 ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag ttttgaagtg
     1621 gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa tctagagtta
     1681 gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt gatgatggtg
     1741 gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc agtcttgcca
     1801 gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc tagtgaagct
     1861 gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt tcaggaacag
     1921 catttctctg ctgtctattt caaaaatgat attgacaagg cttattatct gacaggttat
     1981 gggactagag atcagtttgc caaattgtac aagactattt accagttccc agagtttgat
     2041 attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat cttgctggtc
     2101 aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg tgtgatgaca
     2161 gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca aaatctcaaa
     2221 caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat ttatgatttt
     2281 ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt tttgaaaaca
     2341 gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga ttcggctgaa
     2401 agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat aaactaagat
     2461 tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg tgagaatgga
     2521 aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt agggttaaaa
     2581 tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga ttaccttttt
     2641 gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga tgccattggt
     2701 gctctaccgt atctcttggc agaggctaaa gttcctgtat ttgggtctga gttgaccatt
     2761 gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa tgatttccat
     2821 gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt cttccctacg
     2881 acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg aagcatcgtt
     2941 tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac tgattttgct
     3001 cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc ggccaatgca
     3061 gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac ccaaactatt
     3121 gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc tcgtattcag
     3181 cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg atttgatatt
     3241 gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa cgaaattctt
     3301 ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat tcttgagaca
     3361 ggtcgtatgg gtgagcctat caatggactt cgtaagatgt cgattggtcg ccatcgttat
     3421 gtagaaatca aggatgggga cctagtctat attgctacgg ctccgtctat tgctaaagaa
     3481 gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt caaattgatt
     3541 acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct gatgatcaat
     3601 cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt ggatgctcac
     3661 gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc taaaaagggg
     3721 acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc agcaggagat
     3781 atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg tgaccgtaag
     3841 gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg tgagaagaaa
     3901 attgtggcta gggctcgtgt tcacacgcgt ggatttgttt atctcaagaa gagtcgcgat
     3961 attctccgtg aaagttcaga attgattaac caaacggtag aagagtatct tcaaggagat
     4021 gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa gtatctcttt
     4081 gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa ataatcgttg
     4141 aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag aaggagaaaa
     4201 tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag tggggggtga
     4261 atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat attcccgtct
     4321 tgtacctttt gcacgggatg tctggcaatc ataatagttg gcttaagcgg accaatgtag
     4381 aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc aatggttggt
     4441 acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa ttgccacagg
     4501 ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt atcgctggtc
     4561 tttctatggg aggctacggc tgcttcaaac tggctcttac gacaaatcgt ttttctcatg
     4621 cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt caaaatctgg
     4681 gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca actagtccct
     4741 attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg gcgtggtgtg
     4801 gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc aaaaaactag
     4861 gttttgatgt gacctatagc catagtgctg gaactcacga gtggtactac tgggaaaaac
     4921 aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag agactgactt
     4981 agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc actagacttt
     5041 tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag gaaatgtacc
     5101 gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa catgaattag
     5161 ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa catgagaagt
     5221 ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt agaactcttc
     5281 cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat ggggataaag
     5341 aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag agagcggttt
     5401 ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag gaaaagacag
     5461 aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta aggtggtcag
     5521 gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa atatacaagg
     5581 aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga acagcacgaa
     5641 acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt tcggagttga
     5701 tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat tataaaaaat
     5761 tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat gtttctaaac
     5821 gttcgcaaga attagaagaa cagttagcga aaaatagagc cttggaagag acgtttactg
     5881 agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt ttgttagagg
     5941 aattgacctt cttgcaggaa tatatagatg tagatcaagc gagagttcct ttagcggcta
     6001 ctttgagttt ggaatttggt actacctctg ttaatatata tgctggtatg gatgatgatt
     6061 ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat gcctttgagc
     6121 gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt ggactttatc
     6181 attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt acaatgccca
     6241 ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca ttaagaaaaa
     6301 aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt tcagacttat
     6361 tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga tttgctagaa
     6421 aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat tcaagttgca
     6481 gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa ttcggggcca
     6541 atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga atatgccaag
     6601 caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac ttttgatagc
     6661 caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac tgatttaggt
     6721 tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg gttatactat
     6781 aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa aaagggtaaa
     6841 cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa acgtgaagaa
     6901 ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata tagtgataaa
     6961 agtttagaat attatgagca tttttatgat acttttggag aacaagcgga gtttctcata
     7021 gcaagcttaa atttttcgga ctatatgagc aaattgcaag gtgaacaaag taaactagaa
     7081 gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga gaaaaaacaa
     7141 aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa agcagaagcg
     7201 cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag tttatttgtt
     7261 tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt taataagttc
     7321 tatgcccctg ccctgcttca aaaatatgtt atgttggaaa gcataaaacg tggaatacct
     7381 aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt tttgcgtttt
     7441 aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta ccatccatct
     7501 cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta agatgaaaaa
     7561 gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt gctagaaagg
     7621 tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt tgtgtggcgt
     7681 ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt cggacttctc
     7741 tggtatctga acggagattt tcaaggagcg ctaaagcaag cagaacggtc agtaaaaatt
     7801 ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt aagccagaca
     7861 gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat ttacctggat
     7921 ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa ctggaatcaa
     7981 actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat tacggctacg
     8041 gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac taatctctta
     8101 acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc caatccatac
     8161 tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg tcatgcgatt
     8221 ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt ttatggtatc
     8281 caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta gggtactatc
     8341 tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc aaaactagag
     8401 ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac cattatctat
     8461 gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg aagtcatgat
     8521 accaagacag aggctctggt gtctgaaatt gtggattttg aatttattgt tacggagtct
     8581 aatattgagg cacttctcct agaaatcaac ctgatcaagg aaaacaagcc caagtacaat
     8641 atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga gcgctatcca
     8701 cgcttgatta tcactcgtca ggtcaaaaag gacggaggtc tttattttgg accctatccc
     8761 gatgtggggg cagccaatga aatcaagcgg ttgctggatc ggatattccc ttttcgtaag
     8821 tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggtcaatg catggcccac
     8881 accatctgta agaaggatga gatttatttc aagtctatgg cccaggaggt ttctgatttt
     8941 ctgaaagggc aggataacaa aatcattgat gagctaaagg gcaaaatggc agcggcagcc
     9001 cagactatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc tattggaacg
     9061 cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt ctttggctac
     9121 tatgtggata agggctggat gtgtgtgcag gttttctttg tccgtcaggg taagctcatc
     9181 gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt tttgacctat
     9241 gtaggacaat tctatcaaga aaaatcccat ctagttccca atgaggtact gattccgcaa
     9301 gatattgacg aagaggctgt caaggccttg gtggattcca agattcttaa gcctcaacgt
     9361 ggagagaaaa agcagctggt caatttagct ataaaaaatg cccgtgttag cctagagcag
     9421 aagttcaacc tgctagagaa atctgtcgag aagacccaag gagctattga aaatctagga
     9481 cgcttgcttc aaatcccaac cccagttcgc atcgagtcct tcgataactc caacatcatg
     9541 ggaactagtc ctgtttcagc catggtggtc tttgtcaacg gcaagcctag taagaaggat
     9601 taccgtaagt ataaaatcaa gaccgtagtc gggccagacg actatgccag tatgcgagag
     9661 gtcattcgca gacgctatgg tcgagtacag cgtgaggctt tgactcctcc agatttgatt
     9721 gtgattgatg gggggcaagg tcaagtcaat attgctaagc aagtcatcca agaagaactg
     9781 ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac ccatgaattg
     9841 ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga atttttcctc
     9901 ctccaacgca ttcaagatga ggtccaccgc tttgccatta ccttccaccg ccaactgcgc
     9961 tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc taaacgcaag
    10021 caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag tgtggatgag
    10081 attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaaccaa gttgaacccg
    10141 caggaaacag aaatcttgct tcaagtggcg gaagaaagag tagattacca aacggaagga
    10201 aaccacaata aaccataaaa tcgcaatttt atcagatgtt catggcaatg cgacggcgct
    10261 agaagcagtg attgcagatg ctaaaactca aggagccagt gaatattggc tcatgggaga
    10321 cattttcctc cctggtccag gtgcaaatga cttggtcgct ctgttaaagg aacttcctat
    10381 cacggcaagt gttcgaggca attgggatga ttgtgtcctt gaggctttag atgggcaata
    10441 tggcttagaa gacccacagg aagttcagct cttgcgtatg acacagtatt tgatggagcg
    10501 aatggatcct gcaacgattg tctggctacg aagcttgcct ttgctggaaa agaaagaaat
    10561 tgacggattg cgcttttcta tctctcataa tttacctgac aaaaactatg gtggtgactt
    10621 gctagttgag aatgatacag agaaatttga ccaactgcta gatgcggaaa cggacgtggc
    10681 agtttatggt catgttcaca agtagttgct tcgttatggt agtcaagggc aacaaatcat
    10741 caatccaggg tcgattggca tgccctattt taattgggag gcgttaaaaa atcaccgtgc
    10801 ccagtatgcc gtgatagagg ttgaagatgg ggaattggta aatatcctat ttcgtaaagt
    10861 cgcttatgat tacgaggctg agttagaatt tgccaaatcc aagggttttc cctttatcga
    10921 aatgtatgaa gaactacgtc gtgaagataa ctatcagagg cacaatctgg aactattagc
    10981 aagcttaata gaaaagcata ggtatgtaga ggatgtgaag aatttttttg attttttgta
    11041 agagtttcct aaaataacca atgcaaacta aaaaagcgat tggctgatcc catcgctttt
    11101 aatatatctt atactcaata tgaaaatcaa agagcaaact aggaagctag ccgtaggttg
    11161 ctcaaagcac agctttgagg ttgcagataa agctgacgtg gtttgaagag attttcgaag
    11221 agtgttattg taactgagat tgatctggga ggtaagaacc acctagatag gtattgctga
    11281 gtttttcaag ggttccgtct tgatagagtt ctttgagcgc tttatcaaat tgctctttaa
    11341 actctttttg gtcgcttgag aaaatgatat aattgctggg gctatctgca gaaggtaaat
    11401 caacgactga gaggtctaaa ccacggtcct tgataatctt ttgaacggat accttgtcaa
    11461 aaactaggaa atcaaactct ccgttagcaa ggtctaggat tcgtttacca atatcctcac
    11521 cagaaaaatt aattgtagcg ggattatcag tgtgtttctg attccagtta ttgatgaatt
    11581 gagcgttaga agttccggta tcctcttgtg ttgttttacc agcgatctgg tcaagagaag
    11641 tcaaaggatt tttcttgttg ctgacaagga cgaggggatt gttggaaatc ggaagcgagt
    11701 aaaggtattt ttcagcacgc tcttttgtgt aactcaaatt attggccgca gcctgatagt
    11761 gaccagaatc aagtcctggg aagatacttt cccaggcggt tctttgaaat tgaatctcgt
    11821 agtcgctgag tttttcatct actgccttta gaacttcgat atcgaagcct gtcagattgc
    11881 ctttatcttc gtagtcaaat ggtggcacgt cgccagctgt agcaaggacg attgtctttt
    11941 gagcgctagt ctctttgggt gtagcttgat tcccacaggc aaccaaaaat ggtaggatag
    12001 ctagtaatag gctaaatttt ttcatactgt ctccattcaa atgtaaagta cttgctagtt
    12061 tatcagaaac tttcctgata aaccaatata tatcttttat gtctgtgata gaaaaactta
    12121 atcatgaaaa atccctgcaa gctctttcaa attatggtag aatggaagca gtagaattag
    12181 aaaaggaaat cgattatgaa atttcttgaa ttaaataaaa aacgtcatgc gaccaagcat
    12241 ttcactgata agcctgttga tccaaaagat gtgcgtacgg ctatcgaaat tgcaaccttg
    12301 gcgccaagcg cccacaacag ccagccttgg aaatttgtag ttgttcgtga gaaaaatgct
    12361 gaactggcaa agttggctta tggttcaaac tttgagcagg tatcatcagc acctgtaacc
    12421 attgccctat ttacagatac agaccttgcc aaacgcgctc gtaagattgc ccgagttggt
    12481 ggtgctaaca atttctcaga agaacaactt caatacttca tgaaaaattt gccagctgaa
    12541 tttgcacgct acaatgaaca acaagtcagc gactacctag ctctcaatgc aggtttggtt
    12601 gccatgaact tggttcttgc attgacagac caaggaattg gttctaacat tattcttggt
    12661 tttgacaaat caaaagttaa tgaagttttg gaaatcgaag accgtttccg cccagaactc
    12721 ttgatcacag tgggttatac agacgaaaaa ttggaaccaa gctaccgctt gccagtagat
    12781 gaaatcatcg agaaaagata gaaagaagaa aaaatgacag caattgattt tacagcagaa
    12841 gtagaaaaac gcaaagaaga cctcttggct gacttgttta gccttttgga aatcaattca
    12901 gaacgtgatg acagcaaggc tgatgcccag catccatttg ggcctggtcc agtaaaagcc
    12961 ttggagaaat tccttgaaat cgcagaccgc gatggctacc caactaagaa tgttgataac
    13021 tatgcaggac attttgagtt tggtgatgga gaagaagttc tcggaatctt tgcccatatg
    13081 gatgtggtgc ctgctggtag cggttgggac acagaccctt acacaccaac tatcaaagat
    13141 ggtcgccttt atgcgcgcgg ggcttcggac gataagggtc ctacaacagc ttgttactat
    13201 ggtttgaaaa tcatcaaaga attgggtctt ccaacttcta agaaagttcg cttcatcgtt
    13261 ggaacagacg aagaatcagg ctgggcagac atggactact actttgagca cgtaggactt
    13321 gccaaaccag atttcggttt ctcaccagat gctgaatttc caatcatcaa tggtgaaaaa
    13381 ggaaatatca cggaatacct ccactttgca ggagaaaata caggtgttgc ccgtcttcac
    13441 agctttacag gtggtttacg tgaaaatatg gtaccagaat cagcaacagc agtcgtttca
    13501 ggtgacttgg ctgacttgca agctaaacta gatgcctttg ttgcagaaca caaacttaga
    13561 ggagaactcc aagaagaagc tggcaaatac aaggtgacga tcattggtaa atcagcccac
    13621 ggtgctatgc ctgcttcagg tgtcaatggc gcaacttacc ttgccctctt cctcagtcag
    13681 tttggctttg ctggtccagc caaagactac cttgacatcg caggtaaaat tctcttgaac
    13741 gatcatgagg gtgaaaatct taagattgct catgtggatg aaaagatggg tgctctttct
    13801 atgaatgccg gcgtcttccg cttcgatgaa acaagtgctg ataataccat tgccctcaac
    13861 atccgctatc caaaaggaac aagtccagaa caaatcaagt caatccttga aaacttgcca
    13921 gttgtttctg ttagcctgtc tgaacacggt cacacgcctc actatgtgcc aatggaagat
    13981 ccacttgtgc aaaccttgtt gaatatctat gaaaaacaaa ctggctttaa aggtcatgaa
    14041 caagtcatcg gtggtggaac ctttggtcgc ttgttagagc gcggagttgc ctatggtgct
    14101 atgttcccag actcaattga taccatgcac caagccaatg aatttatcgc cttggacgat
    14161 ctcttccgag cagcagcaat ctatgccgaa gctatttacg aattgatcaa ataaaacgat
    14221 agaagtctga gatcttatgc ttggacttct ttttggaggg aaagtagatg tctcaaatcg
    14281 aaagaatcaa acaggctatc atggcggatt cacagaatgc cagctataca gagcgtggca
    14341 ttgagcctct ctttgcagcg ccaaaaactg ctcgcatcaa tatcatcggt caggctccgg
    14401 gacttaaaac tcaagaagca ggcctttact ggaaagataa aagtggtgac cgcttgcggg
    14461 actggctagg tgtggatgaa gatacctttt acaattcagg ttattttgct gttttgccta
    14521 tggatttcta ctttccagga catggcaagt cgggtgatct tccgcctcgt acaggttttg
    14581 cagaaaaatg gcatccgcag ctcttacagg aattgcctga tattcagtta accctcttga
    14641 ttgggcaata tgcccaagcc tactatttac aggagaaaat cagtgggaag gtaacggaga
    14701 gggtgaaaca ctataaagac tatctgccag cctattttcc gctagttcac ccatcaccac
    14761 gaaatcaaat ctggatggcc aaaaatcctt ggtttgaggc agaagtagtg ccagatttga
    14821 aaaaaagaat taaaaccatt ttatagtcaa tgaaaatcaa agagcaaact aggaagctag
    14881 tcgtaggctg ctcaaagtac agctttgagg ttgcagataa aactgacgaa gtcggtaaca
    14941 tacgcacggt aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt attagaagaa
    15001 aaagaatgaa agaaatagcc tttgacgcat tttaccagct ttaccaaaac gaccagcttt
    15061 ctctagtgga tgtgagagaa gtggatgagt ttgcagctct tcatttagaa ggtgcccaca
    15121 acctaccgct tagtcaattg gctgatagtt atgattaatt ggacaaagat cgcttgcatt
    15181 atattatttg caaatctgga atgagatcgg cgcgtgcttg ccaattcctg ttagaacaag
    15241 gttataatgt tatcaatgtc cagggtggca tgttagcctt tgaagaactt taaaattttg
    15301 catttctcct acttggtgtg gactgggtag gagagtttta tttttagata attcttattt
    15361 ttaagaaaat tgaaaacatt taatatttgc ctcgtgatgc ttttttcaga ctcctaatcg
    15421 tggtatacta ggtcagtatt ttataaatat gaaggagatt tttatggcta aaaaaggtac
    15481 cctaacaggt ttgctcctgt ttggaatatt ttttggtgcg gggaacttga tttttccgcc
    15541 ttctctaggt gctctatctg gagaacattt tcttcctgcc atcgcaggtt ttgtcttttc
    15601 aggcgttggt atcgccgtct tgacccttat tattggaacg ctaaatccta aaggatatat
    15661 ctacgagatt tcaacgaaga tagcgccttg gtttgcgact ctttacctct cagttcttta
    15721 cttgtcaatc ggtccattct ttgctacccc acgtactgct acaacagctt acgaagtagg
    15781 gattagcccc cttttgtcgg atgcaaataa aggacttggc ttgattgtat ttacggttct
    15841 gtattttgcg gcagcctatt tgatttcgct taatccatca aaaatcttag accgcattgg
    15901 acgtgtttta acgccagtct ttgcaatttt gattgttatc ttggtcgttc tgggagctat
    15961 caaatatggt ggaacaagtc ctcaagctgc ttcagctgct tatcaagctt ctgcctttgg
    16021 tacaggtttc ctagaaggtt acaatacctt ggacgccctt gcttcagtgg cctttagcgt
    16081 aatcgcagtt caaaccttga aacaacttgg attttcaagt aagaaagaat acatttcaac
    16141 tatttgggtt gttggtatcg ttgttgccct tgccttcagc gctctttaca tcggtttagg
    16201 ttttcttgga aatcatttcc cagtaccagc tgaagcgatg aagggtggaa caccaggtgt
    16261 ttacatcttg tcacaagcca ctcaagaaat ctttggctca acagctcaac tcttccttgc
    16321 agctatggtt accgtaacct gcttcacaac gactgttggt ttgattgtgt caacagctga
    16381 gttctttaat gagcgcttcc cacaaatcag ctacaaggtt tatgcgacag cctttacctt
    16441 gattggattt gctattgcca atttgggtct tgatgcgatt atcaagtact caattccagt
    16501 actggttatc ttgtacccaa tcacgattgc tatcgttatg attgtcattg tcaacaaatt
    16561 tgttgccctt tcaaaaccag gtatgcagtt gacaattgct gtggttacag ttattgccat
    16621 tgcaagcgta ctaggaagct cgtttaaggt tgagtttctt gcaaatcttg ttagcgttct
    16681 tccttttgcc aaggcatctc tcccatggtt ggtgccagcc attgttggaa tcttgctctc
    16741 attggttcta ccaaacaagc aagaaagcga tgtttttgaa atggaataat cacttaaatc
    16801 acttttgtag ccaagtctac aggagtgatt ttcttttttt atccgatgat aaatgtgtta
    16861 taataggtag cgaaagaggt gaagaaatga atcaaacagt agaatatatc aaagaactga
    16921 cagccatcgc atcgccaacg ggcttcactc gtgaaatttc gaattattta gtcaagactc
    16981 tagaaggttt tggttaccag ccagttcgca cagctaaggg tggtgtcaat gtgaccatca
    17041 aaggtcaaaa tgatgagcaa catcgctatg tgactgccca tgtggatacg ctgggtgcta
    17101 ttgttcgtgc tgtcaaacca gatggtcgtc ttaaattgga ccgtatcggt ggttttcctt
    17161 ggaacatgat tgaaggtgaa aactgtaccg ttcatgtggc tagcacaggt caaaaggtat
    17221 caggaaccat cctcatccac caaacttctt gccatgtcta caaggatgca ggaactgcag
    17281 aacgcacgca ggacaatatg gaagtgcgtt tggatgtaaa agtaagcaat gaaaaagaaa
    17341 ctcgtgctct tgggattgag gtcggtgatt ttatcagctt cgatacacga actgttgtga
    17401 ctgatacagg ttttatcaag tctcgtcatt tggatgataa ggtcagcgca gcgattttgc
    17461 tcaacctgct tcgtatttat aagaaagaga agattgaatt gccagtaaca actcattttg
    17521 ctttttcagt ttttgaagaa gtaggacatg gtgctaattc taacattcct gctcaggtag
    17581 tagaatatct ggctgtggat atgggagcta tgggagatga ccagcagaca gatgagtaca
    17641 cagtatctat ctgtgtcaag gatgcttcag gtccttatca ctatgacttc cgtcaacact
    17701 tggttgcctt ggctaaagag caagatattc catttaagct cgacatctat ccattttacg
    17761 gatcggacgc ttcagcagcg atgtcagcag gtgcagaggt caagcatgca cttcttggag
    17821 ctggtattga gtccagtcac tcttatgagc gcacccacat cgattcagta atagcgactg
    17881 agcgtatggt tgatgcttat cttaagagcg aggcggtatc atagaatgtg cttaatctgt
    17941 cagagaatcg aactcattaa agctgggcaa aatccctact ttgtaaaaga actagaaaca
    18001 ggctatgttg ttattggtga ctaccaatac tttaagggct ataccttatt tttagcaaaa
    18061 gatcatgtca cagaactcca tcatatggaa acttctgtaa aacttcgctt tttagaggaa
    18121 atgagtttag tccaagaagc agtcgccaaa gcatttgagg ctgaaaagat gaatattgaa
    18181 ctcctaggaa atggcgatgc ccacgctcat tggcatcttt ttccccgaag agcaggtgat
    18241 atgaagagtc acggtttgaa tggccgtggc ccagtctggt gggtgccctg ggaagaaatg
    18301 gcggcagaag attgccaagt acaatcgcca gagctggaag agatgattaa aatcttatct
    18361 catgaattgg agaagtattt ggcctaagag agaggaagaa agaaatgaaa aaaagatatg
    18421 tcattttatc tggtttacta gcattgaccc ttgcagcttg ttcgcaagaa aaaaccaaag
    18481 ttgaagaaaa cactcaaaaa acggagcaaa gcagtcaacc ggaaggaact gtaggtagca
    18541 aatctcaagc ttctagtcag aaaaaggcag aagttagcaa taaagggagc tattatagta
    18601 tccaagggaa atacgatgag attatcctag ctaataaacg ttatcctttg tcaaaagatt
    18661 acaatccagg agaaaatcca acagccaagg cagagttgct caaactaatc gcagctatgc
    18721 aggctgaggg ctatccaatc agtgaccaat acagtgggtt taggagttat gaaactcagg
    18781 ctaagctcta ccaagactat gtcaatcaag atggtaagga agcagcagat cgctactcag
    18841 cacgcccagg ttacagtgaa caccagacgg gtcttgcttt tgatttgatt gggacagatg
    18901 gggatttagt tactgaagaa aaggcagccc agtggctctt ggaccatgcg gctgactatg
    18961 gctttgttgt tcgctatctt aaaggcaagg aaaaggagac tggctacatg gcagaagaat
    19021 ggcatctccg ctacgttgga aaagaagcca aagaaattgc tgcaagtggt ctcagtttgg
    19081 aagagtacta tggctttgaa ggcggcgatt atgtcgatta aaaatgaaag ttttctcttg
    19141 catttttaga taaatagtgt ataatggaaa ggtatgtgta aagcatactt gtgggaggta
    19201 aaaatctcta attaccgcca aaaccacaaa ggaggattta aaaatggcta aaaaagtcga
    19261 aaaacttgta aaattgcaaa tccctgctgg taaagctaca ccagctccac cggttggacc
    19321 tgctcttggt caagctggta tcaacatcat gggattcaca aaagagttca acgctcgtac
    19381 agctgaccaa gctggtatga tcattccagt tgttatctca gtttacgaag ataaatcatt
    19441 tactttcgtt acgaaaacac caccagctgc tgttcttttg aaaaaagctg caggtgttga
    19501 aaaaggatca ggtacaccta ataaaactaa agttgctaca gttactcgtg cacaagtaca
    19561 agaaattgca gaaactaaga tgccagattt gaacgcagca aacgtagagt ctgcaatgcg
    19621 tatgatcgaa ggtactgctc gttctatggg attcactgtt gttgactaat caataacaca
    19681 gtagaaaatc tcacagcagt ctattagttg cttttcatac taggcaagtg actgaggctt
    19741 gtacttggat acagcaaggg agcttaaggc cgtaggaaga gaaaaatagt agactgaaaa
    19801 cccgcaagac ttcatcattt cgagaagtga cgtgggagat gaaaatcgat tgaaccactt
    19861 acaaggagaa tagaaaatgg ctaaaaaaag caaacaactt cgtgctgctc ttgagaaaat
    19921 cgacagcaca aaagcataca gtgtagaaga agctgtagca cttgcaaaag aaactaactt
    19981 tgcaaaattt gatgcaactg tagaagttgc ttacaacttg aacatcgacg ttaaaaaagc
    20041 tgaccaacaa atccgtggag caatggtatt gccaaacggt actggtaaaa cttcacgtgt
    20101 tcttgttttc gcacgtggtg caaaagctga agaagcaaaa gctgctggtg cagactttgt
    20161 tggtgaagat gaccttgttg ctaaaatcaa cgacggttgg ttggacttcg acgtagttat
    20221 cgctacacct gatatgatgg ctcttgttgg acgtcttgga cgtgtccttg gaccacgtaa
    20281 cttgatgcca aaccctaaaa ctggtactgt aacaatggat gttgctaaag cagttgaaga
    20341 gtctaaaggt ggtaaaatca cttaccgtgc tgaccgtgca ggtaacgttc aagcaatcat
    20401 cggtaaagta tcatttgaag ctgaaaaatt ggttgaaaac tttaaagctt tcaacgaaac
    20461 aatccaaaaa gtaaaaccag ctacagctaa aggaacttac gtaacaaact tgactatcac
    20521 aactactcaa ggtgttggta tcaaagttga cgtaaactca ctttaatcag tagttaaagt
    20581 aatgtaaaaa agttgaagac actatgtctc aacttttttt gatgtacgac gggcatgtcg
    20641 tatatctgag gtgtaagtcc tcggtgggca cctactaccg gtgaacccaa tagcgattcc
    20701 caagtctgac gctggaaata agaatcgtca gaggaaggga tagcgaaatc gtggctctac
    20761 gaacaggaac gtgataataa ggcctatata gcggataagg tatctggctg gggcgaccac
    20821 tatcaattat aataaaatga aataagaata ggacgaatcg ttcaggacag tcaaatcgat
    20881 ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatctac t
//
LOCUS       DAVMHG010000025        20217 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMHG010000025 DAVMHG010000000
VERSION     DAVMHG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.025"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(24..1631)
                     /locus_tag="VYR08_000714"
     CDS             complement(24..1631)
                     /locus_tag="VYR08_000714"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV3466281.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2021..2608)
                     /locus_tag="VYR08_000715"
     CDS             complement(2021..2608)
                     /locus_tag="VYR08_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV3466282.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2736..3185)
                     /locus_tag="VYR08_000716"
     CDS             complement(2736..3185)
                     /locus_tag="VYR08_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466283.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3190..3579)
                     /locus_tag="VYR08_000717"
     CDS             complement(3190..3579)
                     /locus_tag="VYR08_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV3466284.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3607..4233)
                     /locus_tag="VYR08_000718"
     CDS             complement(3607..4233)
                     /locus_tag="VYR08_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3466285.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4247..4810)
                     /locus_tag="VYR08_000719"
     CDS             complement(4247..4810)
                     /locus_tag="VYR08_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3466286.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(4975..5209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5263..5766)
                     /locus_tag="VYR08_000720"
     CDS             complement(5263..5766)
                     /locus_tag="VYR08_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV3466287.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(5959..6789)
                     /locus_tag="VYR08_000721"
     CDS             complement(5959..6789)
                     /locus_tag="VYR08_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3466288.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6774..8456)
                     /locus_tag="VYR08_000722"
     CDS             complement(6774..8456)
                     /locus_tag="VYR08_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466289.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8558..8770)
                     /locus_tag="VYR08_000723"
     CDS             complement(8558..8770)
                     /locus_tag="VYR08_000723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466290.1"
                     /translation="MKIKEQTRKLAAGCSKHGFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(8777..9325)
                     /locus_tag="VYR08_000724"
     CDS             complement(8777..9325)
                     /locus_tag="VYR08_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV3466291.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9340..10188)
                     /locus_tag="VYR08_000725"
     CDS             complement(9340..10188)
                     /locus_tag="VYR08_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV3466292.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10376..11725
                     /gene="trkA"
                     /locus_tag="VYR08_000726"
     CDS             10376..11725
                     /gene="trkA"
                     /locus_tag="VYR08_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV3466293.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11729..13168
                     /locus_tag="VYR08_000727"
     CDS             11729..13168
                     /locus_tag="VYR08_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3466294.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSNFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13293..14972)
                     /locus_tag="VYR08_000728"
     CDS             complement(13293..14972)
                     /locus_tag="VYR08_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3466295.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGTQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(15041..16453)
                     /gene="lacG"
                     /locus_tag="VYR08_000729"
     CDS             complement(15041..16453)
                     /gene="lacG"
                     /locus_tag="VYR08_000729"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV3466296.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16463..16807)
                     /locus_tag="VYR08_000730"
     CDS             complement(16463..16807)
                     /locus_tag="VYR08_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3466297.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(16975..18861)
                     /locus_tag="VYR08_000731"
     CDS             complement(16975..18861)
                     /locus_tag="VYR08_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV3466298.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(18863..20185)
                     /locus_tag="VYR08_000732"
     CDS             complement(18863..20185)
                     /locus_tag="VYR08_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3466299.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
BASE COUNT         6234 a         4504 c         3493 g         5986 t
ORIGIN      
        1 ttttctcaaa ggttctgatt ttgctaattg ctgttctcaa ccgctgcagt aacaaaggca
       61 gtgtagagtt cttctggtcg gtttggacgg cttgacagtt cagggtgata ctgacaagct
      121 acaaagaatt tattttcagg aatttccacg atttctacca aacgattgtc tggagaaact
      181 cctgaaaaga caaaacctgc tgcctcaaac tgctcacgga aggcattatt aaactcataa
      241 cggtgacggt gacggcgttg caccacttct tgattgtgat aagcagcagc agccttagag
      301 ccacgtttca acttagacgg ataaagtccc aaacgaaggg ttccacccat atcctcaata
      361 tcaatctgat cacgcatgat atcaatgata gggtattttg tttctggtgc aagctctgca
      421 gaattggcac cttcaagacc taaaacgtga cgagcaaact cgatacatgt caactgcatt
      481 cccaagcaga ctcccaacat tggaacatca ttttcacgcg catagcggat ggcttggatt
      541 ttcccttctg taccacgttg accaaaacca cctggtacga tgatcccgtc cgcatcagac
      601 aagagttctg ctacattctc tgctgtcaca tcattggcat tgacccaatt gattttaact
      661 tctacatcat tgacatagcc agagtgtttc aaggcttcga ccactgagat ataggcatct
      721 tgcaactcca catacttacc aacaagggaa atcttaactt gtttcttgag gttcatgacc
      781 ttgtccacca tggctgacca ttctgtcata tccgctgctg gtgcgtctaa tttcaaatga
      841 tcacaaacaa tttggtccat cccttgtgcc tgcaagttca gtggaatttg gtaaaggtgt
      901 tcaacatcca acgattcgat aacggcttct ggtgccacat cacagaactg ggccagttta
      961 tttttaattc cttgaccagc tggctcttct gtacgaataa ccaacatatt tggttggatt
     1021 cccaagccac gcaattcttt gacagagtgt tgggttggtt tggttttcat ttcaccagca
     1081 gccttgaggt aaggaagcaa ggttgtatgg atatacatga cattatccgc acccacatct
     1141 gccttcatct gacgaagagc ctctaggaat ggcaaggact cgatatctcc tactgttcca
     1201 ccaacctctg tgataatgac atcagagtcg gtcgttagag cggcacgctt gattttttct
     1261 ttcaaagcat ctgtgatatg aggaatgact tgaacagttg ccccaaggta ttctccacgg
     1321 cgttctttac gaagaacttc actgtaaatt ttcccagttg tcacgttgga atatttgttg
     1381 agattgatat cgatgaaacg ttcatagtga cccaagtcca aatctgtctc agctccgtca
     1441 tctgtcacaa aaacttcccc gtgctggtaa ggactcatgg ttcccggatc aatattgata
     1501 taagggtcaa acttttgaat ggttactttg agaccacgat ttttcaagag acggcctaga
     1561 ctcgctgcca caatcccttt cccaatggac gataccacac caccagttac aaaaatatat
     1621 ttcgtagaca tagattcctc tttctaaaat gctcaaggtc ttgttaacta cgaggggata
     1681 tagaaaacaa gaccctacta ataagaataa tctgggacga ctgcaccaga ttcacaacta
     1741 atactcttcg aaaatctctt caaaccgcgt caacgtcgcc ttgccgtaga tatgtgttcc
     1801 tgactttgtc agtcttatct acaacctcaa aacagtgttt tgagcagcct acggctagtt
     1861 tcctagcttg ctctttgatt ttcattgagt ataaaataca agaagataac ttcttgaaaa
     1921 aacaaaaata gctccctaag aactagggag ccccgacctc taaaagaggt gcccaaacaa
     1981 tatgatacct aaaaatagga taattgtcaa tagctaactc ttattcctcg ctggtttcag
     2041 catcgtcatc tgaaaactcg tcgtcgtctt cgttgagatc cacatcttct cccaagtcat
     2101 ctggagcgat ttcgttgatt tctgcatcat aagcttccac ttcatttttt tcatcatctg
     2161 gattttcatc atcgtatgaa agagctggat ctgcttcgta tgcatcttcg tcttctggat
     2221 catctgcatt gtagtcaatg gcatctgaat caccatccat aaaggcattg acacgttttt
     2281 tcttagcttt tggtgctact tcatcgtcgt cattttcttc aagagcgatg atttcttcgt
     2341 cgatttcgtc cacaccatac catgaacgaa gaccccattt gttgtcccca agtgagatga
     2401 agctaccgtc aaagttcaac tctgtgtaga acaaaggcaa agcttcgcgg atatcgctgt
     2461 ttgatgttcc aaggtagttt tgaatttcgt ttacaagatc gctaaaatgc atctcatgat
     2521 cgcgaccacg aagttccaag atagcacgcg ctacctcaat catagatagt tcactttttt
     2581 cttgcccagc aaatacttct aattccaaag cgtttctcct catttatact actatcgcca
     2641 gagcgaacag actctgacct cattttatca tttactcttt attttacgat aattttgcgg
     2701 aatagtcaaa ggttaagggg gagaaagtgg caggattaga ctaattccaa tataaaactc
     2761 cttccttttt ctgttgctcc attttccaca aatccaagcg acttgaaaca cctcctagaa
     2821 gcatgattgt aggtgtagat ttccttgact ctcaattctt tccatccttt tactcgagcc
     2881 aattcaatca aagcacttag aatctttttt ccaagtcctc gatgttggta agcggaattc
     2941 ccaatcacaa tggggagatt atcctgagat agtgtaatat ccccaattgg aaaccattct
     3001 cccttctcct tgacttcaat ccaaaaaagc tcaccatgtc gatccaaata ggaatacatg
     3061 gcttccaagg tcgcttgact gtaaggaagc ttcaccccat ctacgaggta aaccaagttc
     3121 acatcctgat accaagcaaa agcttcctca caatgattga ccttataata aggaacaaaa
     3181 cacaatgcac taccttttca acaaaagagt tgctgcttga ttaaaaccat cacaccagtt
     3241 ataccatttt gcttcatacc catcttgagc taggatacga tcttctaaat caaaaacaga
     3301 gtaaatcttt ctttcctcgc aagcttgcgc atagagatga tatagttcat caccaccatc
     3361 tctatcccac tcagcagaaa tcgtatcccg acctgccaat aaagcctgat aagccctgtg
     3421 atgcccatct gtaatcagca aacaatctcc aaaggcaaga atactgattg gatcgacttg
     3481 gtttgtttct gccgactggt aaagcatctg aatatcttgc aacttctttt ctgataaata
     3541 tagttgagtc ggatgaagat ctgctatatt gactttcatt tctttctcct caagggaatt
     3601 cgatactcac ttctgtttgc ctttaaatcg ccattggaag cggagcttgt catagaaggg
     3661 aaactcgata aacaggactc ccaagcccac acagagactg gcaaggacgt ctgatgggta
     3721 atgaactccc agatagactc ttgataccag cacactgact aggtagaggc caaggacgat
     3781 ttgtacgatt tttctccaga ctggattttt aattcgttga ctgagaatga caatcagagt
     3841 gcctaccatc aaggttacag ccagagaatg cccacttggg aaggaaaatc ccttctcctc
     3901 cacaagatgt aagatagctg gtcgtgggcg ctggtagata tttttaaagg tcacgattaa
     3961 aagacctgcc aaagtcagat ttcccagcat gaagaaactt tctatcttcc atcgcttacg
     4021 ataaaagaca aaagctgtaa tgacaaccca agtgataatc actgggatat caatcagacg
     4081 tgtgagggct cgaaaaagaa tagtcaaata atctggtaag tctcctcgaa tggcagtctg
     4141 aatcgattgg tcaaaattga ccagcgtttc agggtaaaat ttgaccatgt agccaagaat
     4201 aacgaaaagt aaaagggcaa aactgccctt cattaaaaat gtttgtttat ctctcataat
     4261 gttttaaggt tggtttcaag agaacataca acaaccagaa tgaaacggaa aagataacac
     4321 cttcaatcaa gttaaaaggt aataccatgg tcattaggta gttggaaagt cccaaaattt
     4381 ttccaatatc aaagttagca aacttagcgt acaaaggaac agcataaaca tagttgagaa
     4441 ccaacatggc caaggttaaa ccaatagttc cagctagaga gcctagtagg aaacgaaggg
     4501 ttgtccgttc ctttttccaa atcaaagcaa atacgatgac aaaaactccc aaagctacga
     4561 tattcatcgg caaaccaatg taagtattca ctccttggct gttaagaagc aatttcaaga
     4621 gtgagcgaag caagagcact cctagagcag caggcaaatc catgaccacc agacccacaa
     4681 ggactggcaa gatactaaat tcgatcttga ggaaagatgc cgctggtaaa agcggaaagt
     4741 caaagtacat cagcacaaat gagatggctg atagaattgc aatggtcgaa agtcgacgtg
     4801 tgtttgtcat aacaggttcc tccaattttc tataaaatca gaagaagttg gaaaggattc
     4861 ctctatctat tctcactttt tatatcccaa aagttccctc ttactctatt aaagaaaaac
     4921 aaagcaagtg gttacaatcc ggctataaat ctatcaaaac agacaaggct attctttcgt
     4981 cttctcccat ccagactata ctgtcggttg tggaatctca ccacatcagc ttgcgctcac
     5041 ggacttcttt aaagagaagg aaatagagat ttttctttta gactaggcga caagctgtag
     5101 gcataacttc tggttaggca aagcttgtca acaaagtata aaaggaaaag atcatttcct
     5161 agttaaagtc accgccggtc gggaatttca ccctgccctg aagactcttt tatcataaca
     5221 aaaaacgctt gcaagcgcaa gcattttgtt cattttcatt ttttatttca atttatccac
     5281 ttcataggta tggacgagct caagacctgc aaagttctgc tggcgaagag cctcatagac
     5341 aatcatgcag acggtattag acacattgag actgcggaca tgttcatcat tcatgggaat
     5401 acggagagcc ttctcaggat gttctcgcat aaaatcttca ggtaagccct tgtcttcacg
     5461 tccaaagaga aaataatggt cctcgtcagt cgataaatcc acctcagaat agactttttc
     5521 cgcgaatttc gaaatcagat agagttttcc cttcatctga gatatgaaat cttccaaact
     5581 ctcgtaaaaa taaatctcta gcttatccca gtaatccaat ccagcccgct tcatcttgcg
     5641 gtcatcaata ggaaagccca taggcttgat gatgtggagg ggagaattgg tcgcagcgca
     5701 agtacgcgcg atattgcccg tattttgtgg aatttgaggt tcaaataata caatgtgatt
     5761 tgtcatgact tgcttccttt caccattgca aaaaaatagc cacactgccc ggagtcaagc
     5821 tcagcaaaca gcgtggttaa ggcatcgtta acttacctca caacaggttt gaagtaaatc
     5881 agcgaaacta ctttcttagt ataacacttt cagaatcatt gtcaatagaa atgacttgat
     5941 tttttcaatt ttttcaagct atttccaagg gttgtaaaat cgtccctgat tctgcaagat
     6001 aagtagtaaa ctaactacta aaaacaaggt tgccaagagc aaggtaatat agtctccttt
     6061 tttcaaggcc tgataactat accatgtgcg ttttttctct ttcccaaagc ggcgaagctc
     6121 catggcagtc gcaatggtat caatgcgttc tagcgagcta aaaatcaagg gcgtaataat
     6181 gagcagattg cctttgattc gttgcataag agaagctttc ttggataatt ccatcccacg
     6241 cgcctcctga gacatcttga tagtaaagaa ttcttcctgc aaatctggaa tatagcgcaa
     6301 ggtcaggctg acagaataag caatcttata gggcacacca atttgattta aactggaagc
     6361 aaactgacta ggatgggttg tcatcaaaaa gataatagcc agaggaatgg tgcaaagata
     6421 cttaatggcc aaatttagca gataaaagag ctcctggctg gttagagtgt agacaccgat
     6481 tccctgccaa atcacacttc tctctccata aagtccaacc ccatactcgg gagaaaagag
     6541 atagaccatc aaaacgttta aaacggcaaa tatcgtcgca aaaacggcta caaaggaaac
     6601 atctttaaag cgaatttctg ataaatagag gagaaagact gaaaagatgg caatcagcaa
     6661 gagcattctg gtatcatagc taatcatggc cgccaatgat accagaatga aaaagagaag
     6721 tttcccagct cctgacaagc gatgaatcac agtatctcta tgctggtaac cgattaattt
     6781 agcttgcatc cctctctcct ttctttgtaa aatgccgtta aatccagtgg atccacatct
     6841 agtttcttag ccaagttaaa gatggaggtt tcttttagat tggcttttac taacagctca
     6901 ggatcgctca acagactggc tggaacagta tcggcaatca attctccatc caccatgaca
     6961 aggacccggt ctgaataatc cagcatcaat tgcatatcat gggtaatcat gacaatggta
     7021 tgcccttttt gatgtaactc ttcgagaaat tccataatct cagtatagtt cttctgatct
     7081 tgacctgcag tcggttcatc taggagaata atttcagctc ctaagaccaa aattgaagca
     7141 atggtgacac gttttttctg accaaatgac agggcagaaa taggccaatt acggaattca
     7201 taaagtccac agattttcaa ggtttcatat actctcgttt caatttcctt ctcatccaca
     7261 cctcgcaaac ggagccctag agccacctca tcaaaaatca tattggttga aatcatttga
     7321 ttaggatttt gtagcacata tcctactcgt tccgcccgct ctgcaacaga atcgcctttt
     7381 atatcctgtt tttcccaaag atagcgtcct tccgtctgaa taaagctact tatagccttg
     7441 gctagagttg atttccctgc tccatttttt ccgacaatag caatcttttc acccttttta
     7501 atatctaaat gtagggattt taaaatcggt ctatcatcat aagaaaaaga tacttcctct
     7561 agtctaaaga gtgactgcaa tgctggggtt tcttttgcca gttcattctg caactgaacc
     7621 tgaccttttg agatagacaa gttatccaga ttcgctaatt gttcttcctt gactaagtcc
     7681 acacctaatt gacggagagt cgttagataa aggggttctc gaattccatt ttgagtcaat
     7741 aaatcagtcg caagcaactg gtcagggctc ccattaaaaa ggatacgacc atcgtttatc
     7801 aagacaatcc gatccacagg gcgatgcaga acgtcctcca aacggtgctc gataataaga
     7861 gtcgtcgtcc cctcttcctt atgaatctgg tcaatcaatt cgataatatc ctgacctgac
     7921 ttgggatcta gattggcgag tggctcatca aacaagagaa tcggactttc atcaatcaag
     7981 acaccagcca gactgactcg ctgcttttgt ccacctgaca aatcctgagg acgctgatcc
     8041 agtaaaggaa gaaggtccag cttttcagcc catttataaa cacgaccttt catctcatct
     8101 agggctgtca catcattttc cagagcaaac gccaaatctt ctgccacaga caagccaata
     8161 aactgcccat ctgtatcctg caaaactgtg ctaaccagat gagacttatc atagatgctc
     8221 atatcaaagg ctacttgacc ctttatcaaa aattctccat atgtctgacc cttgtaaata
     8281 ttgggaataa tcccattcaa acactgaccc aaggtagatt tacctgaccc agatggtcca
     8341 acaattaaga ctttctctcc cttgtaaatg gtcaagtcta tcccttgcaa ggtcggttct
     8401 tgttgtgttt cataccggaa agagaaatcc ttccactcaa ttatagcttc tttcatctta
     8461 ctctcttcat tcgcttctta gacttctatt ttatcataaa tcaagccctt cttgcagtct
     8521 ctcctcttaa aatcttagcg ccaaaaagat tcctatccta gcttacttgc ctaactaatc
     8581 tataaacatc gaaaaagact agttgcccag ccttccccat cattttatac tcttcgaaaa
     8641 tctcttcaaa ccacgtcagc ttcgccttgc cgtaggtatg gttactgact tcgtcagttt
     8701 catctacaac ctcaaaacca tgttttgagc aacctgcggc tagcttccta gtttgctctt
     8761 tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat
     8821 aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa
     8881 caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg
     8941 gtgccaagac accccaaaca agggcatttg caagtagttg aatgagatta aaaataagaa
     9001 tatctttcca gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa agtcccacaa
     9061 ctaaaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa ccaacaagag
     9121 agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa
     9181 tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg
     9241 gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt
     9301 gtttaattgt aaatttgatt tccatactat tctcctattt tatccttcta ttttctttat
     9361 ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt
     9421 gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac
     9481 atctgcaaat gatttgccat agacaacctg attttgatag accagcatat cagcatgata
     9541 gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt
     9601 gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag
     9661 atgatcttct atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc
     9721 tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata
     9781 gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc
     9841 agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt
     9901 atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga
     9961 accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt
    10021 ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg
    10081 atgtattttt aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc
    10141 atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg
    10201 tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag
    10261 ggtatagttt taccttatag cgaattttca ctaaaaactc ttgttattag ctagtaggtg
    10321 cattttttct tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa
    10381 aattatcctt gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga
    10441 aaagcatgat gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg
    10501 ctttgatatc attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag
    10561 cgtccaagat tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc
    10621 agcagttcta gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga
    10681 atattctaac tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc
    10741 tgagctcttg gctgcccgcg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt
    10801 cgaacgcttt gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg
    10861 tctttgccaa atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc
    10921 gatagagagg gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga
    10981 tagaatcttt gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc
    11041 acgcgccgtg aagagccttc tcatcgttgg ggcaggtaga attgcctatt atctacttgg
    11101 tattctcaaa gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag
    11161 cttctttagc gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga
    11221 tatcctgctg gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga
    11281 tgaggaaaat ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac
    11341 taaggtcaat cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat
    11401 cacacctaaa agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc
    11461 ccagtattca gaccttcaag ctatgcacca tctagccaat ggccaaatcg aaaccctgca
    11521 attccatatc aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa
    11581 aaaaggggtt cttattgcag ccattattcg aaagggcaag actattttcc caactgggga
    11641 ggatatgttg gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa
    11701 gatttatgac ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc
    11761 aaagttactt ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta
    11821 ccgtgaatcg agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt
    11881 aggcggttca gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt
    11941 cttgatcgtt gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt
    12001 ttctgggcaa atccctagcg ttattgatgc cttttttgaa attagttctg ggtttacaac
    12061 tactggagca agtattttga acgacatttc ggttctcagc cgttccctcc tcttctggcg
    12121 aagttttacc cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga
    12181 caatgccaaa aatagtcacc tagaagtaat gaaggctgag gttcctggtc ctgtctttgg
    12241 taaggttgta tccaaattaa aaaacactgc ccagatcctc tatctccttt acatagccct
    12301 cttttccctc tttgtcatca tctattatct agccggtatg cctctctacg atagttttgt
    12361 cattgctatg gggacagcgg gaactggagg atttaccgtc tataacgatg gaattgccca
    12421 ctatggtagc tcactgatta cctatctggt tagtatagga gttttggttt ttggggtaaa
    12481 tttcaatctc tactactacc ttatgctccg tcgggttaag gccttctttg gagatgaaga
    12541 gcttcgggct tacttggtca tcgtgctggt ttctacaggc ttgattaccc tcaatactct
    12601 ctacctctac ccaggatttt ccaaaagctt tgaaatggcc ttcttccagg tttccaacat
    12661 cattacaaca actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt
    12721 tatcctcctc ttcctcatga caatcggtgg ttctgctgga tcaactgcag gtggactcaa
    12781 gattattcga ggcctcatcc tttcaaaaat tgccaaaaat caaattttgt ccattctatc
    12841 tccccaccgt gttttgactc tccatgtgaa taaaacggtg attgataagg atacccagca
    12901 taaaattctc aagtactttg tcatctatgc tatgattttg ctatccctta tctttattgt
    12961 cagcctagat agcaatgatt ttctgatcgt gaccagcgct gtctttagct gtttcaataa
    13021 tatcgggcct attctaggaa ccacttctag tttctcaaac tttagtccta tctcaaaaat
    13081 tctcctctcc tttgcaatga ttgcaggccg cttggagatt tacccaatcc tacttctctt
    13141 tatgaagaga acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa
    13201 gagggatttt tatattaaaa agggggaacg tttttaaatc caagcctgtc tgtaagcaaa
    13261 gttcttactc aaataaaaga tggaaaaact tactacacag cttggtaatg ctccaataca
    13321 aattgtaagg ctttacttgg attctttgtt aaatgaatat attccattcc tctggtagca
    13381 actatctgaa tacctaatct ttctgcatcc accttcatct ctctataata actccgaact
    13441 tgtggagcca gaataattaa atcataaaca cccataatat cataatgagc tccgtacgct
    13501 cctgaattcg caatcactct aacttctgct aattgagtgc cctcgttaat tgcattggct
    13561 aattgcgcac ttgttccaga ccctgcacaa agaaccagta ctttcaactc cttacttttt
    13621 attacatcta tctcaccagg cattatttca cttgtatcct cctctaaaat aatatcattt
    13681 gagcttgcaa tatcttcttt caccagtaac tgtctatcat acgctctaca gaatggcaaa
    13741 taaatcaata tgtcgacaac taaaatcaaa gataaaaata caaaggagat aagttgaaaa
    13801 ttcgttccaa ttaacaatcc caagggacca ggaaaggtcc aaggtaactg gatataaaat
    13861 ccattcattc caaagaaatc aataaagacc tttcctagaa atacattcac tggtggagtc
    13921 atcaaaaaag ggacaaaaag atagggattc aaaataacag gcataccaaa tagaagaggt
    13981 tcatttaccg caaataaaac aggagtaatt gtagctttac ctaccgattt taattgttta
    14041 gaccgcataa agaaaatcaa aataaatggt acaacaaagg tagcccccgt tcctccaata
    14101 gcagcgatat aattcccgaa attaggtgtt aaagaatgat aagggaactg cccatttgca
    14161 agaagttgag cattctcttc catattgctc agttgcaaag ctgtaacagc aggtaagaca
    14221 atagatggtc catgaattcc aacaaaccaa agcaaagcac acataaacca aatcaacatc
    14281 atagcaggat atgattctgc ccccttaaaa ataggagtca atagttgttg aaatacttgg
    14341 gcaaaaggaa catctaaact aaaccgagat acaatatcta acaaaccact aataagtaaa
    14401 acaaaagaaa aagggaaaat atctctaaaa gcttgtgata tagccccagg aacttcctta
    14461 ggtaaatgaa tagtaatgtc tcgcttaata cagaatcggt aaacatttac agtaataaag
    14521 gcactgacaa acgaagttat caatccttga gatcctaaat agtttgtcga caaacttgta
    14581 gatcccgtct tctcatcaac tacaagcgtt acagttaata ccagataact acatatggct
    14641 gccaacattg cagaaatatc atttattacc tttccgtgag gcatttttct gttaacattt
    14701 ccaactaatg actttccaac agtccctgaa acaataatac ccaaaactcc catggttaac
    14761 atatacactt ttctcatcca gacaatagct ggactcggta actctactcc gaaattttta
    14821 ggaatcataa taataatcat caaaaagctt gaaaacataa ttaaaggcat agtagcaatg
    14881 aatccatctt taatagccat catatagtta ttagtagaaa ttttttcaaa tttcccttgt
    14941 tgcttttcaa tggcagcgac tagcttatcc ataaaactcc tcctataaaa aattcaacct
    15001 catatttaaa acatattaaa catcagtcaa atcgctaacc ttaattccgt tttagccctt
    15061 ttacccaaag agcacttttc ttaatatatc tcttctgtgt ttcataatca acaaagaaaa
    15121 gaccatatcg cttattgtag ccattcgccc aagaaaattg atcttgcaaa gaccatataa
    15181 aatagccttg aatattgact cccctatctc gagcctctaa aactttgtgt aaatgttgct
    15241 caatataacg gattctttta ctgtcatcaa taatatcatt ctcgccctct ggcttaacct
    15301 ctttaagggc tgtaccattt tcagttaaat aaatgaccgg atgttgagga tattcttctt
    15361 tgatacgaag caacatatca aataaccctt gaggataaat attccaatcc caatctgttg
    15421 tcggaatacc aggctttttt acaaattcac ctagagcatt taatttaaaa gaagactgtc
    15481 cttttattcc tgttgaatta aattcttgtc tattttcacc tttgtattca cgaataaatt
    15541 ctgaacgata ataattcatc ccaaacatcg tattaagagg tgcagctctt ttcataattt
    15601 ctaaatcacc atcttcaata ataaagctag aattattagc ttccaatatt tgattcaagt
    15661 gaaaaagcgt gtcatcactg tagtagccca aaaaagttcc atctaataga aatttattat
    15721 tataaacatc ataccgttta gctgctaaaa tattttcttt ttgcccatca ataggatagc
    15781 ctggctttaa agcatgaata caacctatct ttccctcaat ccctaattga tgaaattcga
    15841 ggactgcaag agcatgcgcc aacaacatat tatgattcgc ttgaattgct tcagataatt
    15901 gaaaatgatg atttggagga aactgacctc ctatatattg acctgcagca agagacatca
    15961 gttcattgat tgtaaaccaa tgcttgactt ctgtaaattc ttggaaacaa aagcgagcat
    16021 atcgtatgaa acgatcaata ttctctctat tcaaccaatc ccctgtttct aacattttct
    16081 gaggcgaatc aaaatgatgt aaagaaacaa acggaatcac attatgtttt aagcaagact
    16141 gaaagactct atggtaataa cgaacagcta atatattagg ctccccatct atatcaggaa
    16201 atatacgaac ccaagaaata gataaacgca aagcctgcaa accatgttct gccgccaaag
    16261 ctatatcctc ttcgtaacga taataaaaat cactagctgg atctggtaag aacggactgt
    16321 tttcttgcaa ataaacatcc cacatatttg ttcctttgcc atctacccta gttgcaccct
    16381 ctacttgata agcagcggta gcagctccta ataaaaactc cttcggtaag gctttaacct
    16441 gtaaattttc catgactaat cctcattgca aaccaatatg ttcttcaatc tttcgaattc
    16501 gttcatattc atcaataaaa aacttgacct gatcatacaa tagcatcgtt gtcatcaaag
    16561 tatcttgacc atgaatcatg ataaaactca cttcaaaatt atctccagat gcctcctccg
    16621 ctaataaatt agtctgttct cgatgggcag acactattga gtcgtttgca gactcaacca
    16681 attctcttgc ctgttcaaaa tatccatctc tcgcaaacgc caaagcatcc atcaaatctg
    16741 accttgcatc tcctgcgtaa gcgacaattg taaaaccaag catatttatt tcttcttttg
    16801 atttcatctt tcttcctcct caatcttgat ccatctttta cacatcatta ttaaaatgac
    16861 ttcgctacga tataaataga aagtcgaccc taaatatctt aaaaatcctt aaatttcata
    16921 atatttagga tcaatcttct ctgaatacct tggttcattt tctatactta tgatttataa
    16981 gtggaaacca gcgtaatttt ttgctacttc aattacacga taaacatcca aattagattg
    17041 gaatagcagt tcgtgcccac ggatataatt caacacattt ctccattcat cttgagcttg
    17101 ccttgttttt ccggttgctt gaagatataa agccttcgcc atcttaacaa catattcacg
    17161 atgataactg agctgtttcc attcatcctt ttgactattt aataacttag aaatattttc
    17221 ctcaataatt ggcaaaaaat tatcagctag attgtaagct atataataat gattcgctaa
    17281 aacatcattt tgacgactgc caattgcatt aaaataatca catgaggaat aactggataa
    17341 tttttctaaa tattcaacaa cagactgcca atttgaccca tacaaagcag aaaagtattc
    17401 ttcaatcaat tcttcataac ttcttgtctt cttccagagc atttccccca taacataatt
    17461 aggaaaatta tgagggaatc cggcacgtaa ttcttgacac gaaatgtaac cgttcaaatg
    17521 taggttagaa agataagata catctctgta aatggtttga ctaattttca tatagcctaa
    17581 atcgccataa tgagcacgcc ctaaaggata gtcatatacg aaactatctc ctttaaatgt
    17641 tttttgccac tcaaaaagat aagataaatt ttcctcaaga gaattcggaa gtataatttt
    17701 attacgcaga taaggtttag gcgtaggtat ggaattgtca aaatctacat ctgcataact
    17761 catttcaaat gttcttgtaa tcggtgcaaa catcatggta aagcgttcag gattatctaa
    17821 tttttctttc tgaggcgccc atagcaactc atggtaaagc agaaaacaaa tctttgtatc
    17881 taatccctca ctcgttaaag ccctatccaa ttgattgaga atacgaatat actgatccga
    17941 aaccaattct tgtctacagt tttcgcattc acaaatatta ttacgagcat ccgacaacca
    18001 tacatgtaag tagttaacat caggtctttt cttggcataa tccttgataa tttctaccat
    18061 cttatccgcc acatctggat ttgaaaaatc cagactggtt aaaatcggag ccgtattaaa
    18121 taattctcgt ttcccgttta tttcagcgac atacggtttc ttctcctctg aaatactaag
    18181 acctgattcc cagccaaatt ttgaagagta acctaaaacc tcacctgtcc atccatgacc
    18241 aacacgatga tgaataagac ctcttttttg caattcttta tccaacctat cactcaattc
    18301 ttgtactaat tcatttgaga atttttcttt atttaggtat ggattaaact catgttcata
    18361 ccaacgtttc aaaaaagagt aaggattttc aaactggatg aaaaaactgt tcatcccaat
    18421 cttaggtagc caatcaatga aatctagtat attttcaaat gaatccgctc cctctataca
    18481 tactccccta tgatagtaac tggctgtttc atctatagat agctgtttat ctaaaaaatc
    18541 ttcaaatcgt aactctggaa caaagtcatg tctgcgccct ggtctagtat acacaacccc
    18601 aaattcatga aacattcggt aaattccaat aagtaaacct acaattgtat tagactcaat
    18661 tctcccctta cccttgtcta atgagataag aacatggtct tttctgtcct ctttattgac
    18721 taatcctaca aaaatatccg cttccttctc ctcgacaagc tctatgttag tcttaaagac
    18781 ccgattcata tagtaactta attcctctaa ggcaaagcta gaataagtat ttttctgttg
    18841 atcaaatact ctgataacca tcttatttcc ctccaacagt agctttttct ttttcaagat
    18901 tgcgtttatc tgcaattttg aaaaatggat aataaattgc tacagaaacc aaaatcaaaa
    18961 caacttgtaa tagagctcct cgccaactag ctcctgtcgc aaggaagcct cctatgatcg
    19021 gtggcattgt ccacggaagg attacacctg ttgtataggg tactaatcct acagccattg
    19081 atacataggt aatcaaggca ttgattgtag gagtagcaat aaacggaatc agcataatcg
    19141 gatttaaaac tgttggaaac gtaaatagaa tagctgtatt gatattaaaa atagacggta
    19201 taatagctag cttacctaat gttttattcg cacgactctt actaaatagg aagagacaaa
    19261 tcgcaagacc aatagtcgct ccaccgccac caataaatac aaataaatct ttaaacggta
    19321 atgtaataat gtgttggagt gtttgtcctg cagccacagc ttcttggttt tctgtagtaa
    19381 attgtaacca aactgggtct acaaaagcat ttaaaacttg tcccccatta actccacaga
    19441 accaaaagaa tgagtttaca ataacacata gaatcatacc tggaagcgtt cctgcaatta
    19501 acttaagcgg tgttccaaca attgcaccta ggagtccgtt gagacctcct gcaactcctg
    19561 ccgcttcaag accttttaag accagagccc acaaaacaaa agtagtaaag ccaggtaaaa
    19621 gagctgaaaa tgatttactt accacatctg ggacaccact tggcattttt atcgtaattc
    19681 cgcgctggat aaacatacga taaatctctg ctgtaaccaa tccaatagta atcgccataa
    19741 acaaagaaga tgcattcagg ctagaaaatg gtattgcgcc gccaaataac tgcttgacct
    19801 gctccccatt tttatcataa accatactcg aaaaacgagg tgccatcaat acaaaactgg
    19861 ataaggctat gatccctgcc gacggaccat ctgtaccata tccttccgac aacctgtagg
    19921 caataccgaa acaagcgacc aaggagataa tcgctactgt tgactgattc atagttgtca
    19981 agatacttag caatccaaca ctattcagcc aatttgtaaa agcctcttga ggccaactga
    20041 ttagaataag aaataaagag ccgactaaaa tcaaaggcat tgccatcata aatccttcac
    20101 gaatagcttt caaatgccgt tggttgccaa ctttttcacc aaacggagcg acttttgttt
    20161 ctaaaaacgc taaaatattg ttcatcaccg gaactccttt ttttattttt tataaat
//
LOCUS       DAVMHG010000026        19530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMHG010000026 DAVMHG010000000
VERSION     DAVMHG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.026"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(6..974)
                     /locus_tag="VYR08_000733"
     CDS             complement(6..974)
                     /locus_tag="VYR08_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3466300.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNEKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            1110..1967
                     /locus_tag="VYR08_000734"
     CDS             1110..1967
                     /locus_tag="VYR08_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV3466301.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            1954..2733
                     /locus_tag="VYR08_000735"
     CDS             1954..2733
                     /locus_tag="VYR08_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466302.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEASITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            2757..4109
                     /locus_tag="VYR08_000736"
     CDS             2757..4109
                     /locus_tag="VYR08_000736"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV3466303.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            4183..4557
                     /locus_tag="VYR08_000737"
     CDS             4183..4557
                     /locus_tag="VYR08_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV3466304.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            4559..5407
                     /locus_tag="VYR08_000738"
     CDS             4559..5407
                     /locus_tag="VYR08_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3466305.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            5673..6440
                     /locus_tag="VYR08_000739"
     CDS             5673..6440
                     /locus_tag="VYR08_000739"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV3466306.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            6452..7636
                     /locus_tag="VYR08_000740"
     CDS             6452..7636
                     /locus_tag="VYR08_000740"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV3466307.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            7633..9504
                     /locus_tag="VYR08_000741"
     CDS             7633..9504
                     /locus_tag="VYR08_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3466308.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMVNQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(9647..10831)
                     /gene="mntE"
                     /locus_tag="VYR08_000742"
     CDS             complement(9647..10831)
                     /gene="mntE"
                     /locus_tag="VYR08_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV3466309.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            11135..13831
                     /locus_tag="VYR08_000743"
     CDS             11135..13831
                     /locus_tag="VYR08_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV3466310.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            13844..14635
                     /gene="yghU"
                     /locus_tag="VYR08_000744"
     CDS             13844..14635
                     /gene="yghU"
                     /locus_tag="VYR08_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV3466311.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            14751..15161
                     /locus_tag="VYR08_000745"
     CDS             14751..15161
                     /locus_tag="VYR08_000745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3466312.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            15173..16768
                     /locus_tag="VYR08_000746"
     CDS             15173..16768
                     /locus_tag="VYR08_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466313.1"
                     /translation="MKRIVCELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            16947..17111
                     /locus_tag="VYR08_000747"
     CDS             16947..17111
                     /locus_tag="VYR08_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV3466314.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            17190..17918
                     /gene="yaaA"
                     /locus_tag="VYR08_000748"
     CDS             17190..17918
                     /gene="yaaA"
                     /locus_tag="VYR08_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV3466315.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(17969..19453)
                     /locus_tag="VYR08_000749"
     CDS             complement(17969..19453)
                     /locus_tag="VYR08_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466316.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILEGLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT         5694 a         3679 c         4463 g         5694 t
ORIGIN      
        1 tttttttata aactagtaga gtgttttggt tgtacttttt gttcagggtc aatgtagttg
       61 atagcgttgt tgacagcagt tggagcttct ccgaggcctg tcgcaatcag atcaattttt
      121 ccgtcatagt agcagcagtc accgatagca tagatacctg cttggctgga ttcctgtttg
      181 ctgttgacga taatcttgtg acggttgagg tcgagccccc agtttttaag gttaccgaca
      241 gaagatttga aaccatagtt gacaaagagg tggtctaggt caatggtttc agtttcatca
      301 gatttgactt ttgtgatttc aagtttatca agtgtttttt catttccaag gagttggcta
      361 ggggcgaatg gtgtcttgat ggttacagat gattcttgca aggcttgaac actgtgttcc
      421 aaggcacgga aattatctct gcggtgaaca agggtagttg gtgcgatttt ttcaaaagcc
      481 aaagcccaat ccacagccga gtctccccca ccaagaatcg tcactttctt accagcgtat
      541 tgctgaatgt tagaaacgtg gtagtggata ttttcatagc cctcaacacc ttcaagttcc
      601 agcggacgtg gtttgaaggc accgccaccc atagcgatga tgactgtttt agtcaggtga
      661 cttcctttag aagttgtgat ggcaaattct tcttcttgtt tgtcaatctc aagaaccgtt
      721 tcattgagat gaataggggt atcaaatcca tttagctgtt caatcaagcg gttagtcaac
      781 tcttctccag tcaggtttgg gaagcctggt acgtctagga tttccttttc agggtagaga
      841 atagcaggtt gtccacctag ctggggaaga gagtcgatga tttgaacctt ggcttggcgt
      901 aggtgggcat aaaaggctgc aaaaagcccg acaggaccac cacccacaat ggtaatatca
      961 tagagttgag acatagtttc tcctttgttt tttctagtca gtttatttta tcatattttt
     1021 gttcaatgta aaatggacta ggatagggaa aaaaatgcca gaaaatggta taataggacg
     1081 aaagtgtttt gatagggagg atatcaggga tgaattttca acaattatcc aatctgcaat
     1141 attggaccag tttgtttgca agtccatgga cgatagctat caatctgatt gatattttga
     1201 ttgttgctta tattttatac cattttacaa aagctattgc aggaaccaag attatgattt
     1261 tggtacgtgg agttttggtg tttattttag ctcaaatcct tgcaaatatg attggtttga
     1321 ctacgatttc ttggttaatc aatcaaatta ttacttatgg ggttattgcg gcggttgtta
     1381 tcttctctcc agagattcgg actggtttgg aacgtttggg aagagctaca gatttttttt
     1441 ccaatgcccc tattagtgct gaggaacaga tgattcgtgc ctttgttaag tctgttgaat
     1501 acatgagtcc tcgtaaaatc ggggccttgg ttgctattca gcgtgtacgt accttgcagg
     1561 agtatatttc gacaggaatt cccttggatg ctaagatttc tgcagaactt ctcattaaca
     1621 tttttattcc caacactccc ctacatgatg gtgcggtgat catcaaagaa gaacgtatcg
     1681 ctgtgacgtc tgcctatctg cccttgacaa aaaacacagg gatttccaag gaatttggga
     1741 ccagacaccg ggcggctatc ggtttatcag aagtctcaga tgctttgact tttgtcgtat
     1801 cagaggaaac gggaggaatt tcgataacct ataatggaag gtttaagcac aacctaacac
     1861 ttgatgaatt tgaaacagaa ttacgtgaaa tcttacttcc aaaagaggaa gtgggtctta
     1921 gttttaaaga acgattgcta ggaggatgga aacatgaaaa aaaatagttt atatatcata
     1981 tcctcactct tttttgcttg tgtcttattt gtctatgcta cggcgacgaa ttttcaaaac
     2041 agtaccagtg ctaggcaggt aaaaacggaa acctatacta atacagtaac aaatgtccct
     2101 attgacatac gctataatag tgataagtat tttattagcg gttttgcttc agaagtatca
     2161 gtggtcttga ctggtgcaaa tcgcctatcg ctagctagtg aaatgcaaga aagtacacgt
     2221 aaattcaagg ttactgctga cctaacagat gccggtgttg gaacaattga agttcctttg
     2281 agcattgaag atttacccaa tgggctgacc gctgtggcga ctccgcaaaa aattacagtc
     2341 aagattggta agaaggctca gaaggataag gtaaagattg taccagagat tgaccctagt
     2401 caaattgata gtcgggtaca aattgaaaat gtcatggtgt cagataaaga agcgtctatt
     2461 acgagtgacc aagagacatt ggatagaatt gataagatta tcgctgtctt gccaactagc
     2521 gaacgcataa caggtaatta cagtggttca gtacctttgc aggcaatcga ccgcaatggt
     2581 gttgtcttac cggcagttat cactccgttt gatacaataa tgaaggtgac tacaaaacca
     2641 gtagcaccaa gttcaagcac atcaaattca agtacaagca gttcatcgga gacatcttcg
     2701 tcaacgaaag caactagttc aaaaacgaat taaaaataga aaaggatttt ataacaatgg
     2761 gtaaatattt tgggactgat ggagtccgtg gagaagctaa cctagaacta acaccagaat
     2821 tagcctttaa actaggacgt tttggaggct atgttcttag tcaacatgaa acggaagcgc
     2881 cgaaagtctt tgtaggacgt gacacacgta tttcagggga aatgctggaa tcggccttgg
     2941 tggcaggtct cctttcagta gggattcacg tatacaaact tggtgtcctt gcaacaccag
     3001 cagtagctta cttggttgaa actgaaggag caagtgccgg tgtcatgatt tctgctagcc
     3061 acaatccagc tcttgataac ggaatcaagt tctttggcgg tgatggcttc aaactagatg
     3121 atgaaaaaga agcagaaatt gaagccttgc tagatgctga ggaagacact cttcctcgtc
     3181 caagtgcaga aggcttagga attttggtag attatccaga aggcttgcgt aagtatgaag
     3241 gataccttgt ttcaactgga actcctcttg atggaatgaa ggttgccttg gatacagcta
     3301 atggagcagc ttctaccagt gcccgtcaaa tctttgcaga ccttggtgcc caattgacgg
     3361 ttatcgggga aacaccagac ggtcttaaca tcaaccttaa tgttggttca acacatccag
     3421 aagcccttca agaagtggtc aaagaaagtg ggtcagctat tggtttggcc tttgatggag
     3481 acagtgaccg cttgattgct gttgatgaga atggtgacat cgtcgatggt gacaagatta
     3541 tgtacatcat cggaaaatac ctttctgaaa aaggacaatt ggctcaaaat acaattgtga
     3601 caactgttat gtctaacctt ggtttccaca aggccttgaa tcgcgaaggt attaacaagg
     3661 cagttactgc agttggtgac cgctacgttg ttgaagaaat gagaaaatca ggttacaacc
     3721 ttggtggtga acagtctggt cacgttatct tgatggatta caataccaca ggtgatggtc
     3781 aattatcagc agttcaattg actaaaatca tgaaggaaac tggtaagagc ttatcagagt
     3841 tggcggcaga agtaacgatt tatccacaaa aattagttaa tatccgagtg gaaaacgtca
     3901 tgaaggaaaa ggccatggaa gtgccagcta tcaaggccat catcgagaag atggaagaag
     3961 aaatggcggg gaacggccgt atccttgttc gtccaagtgg aacagaaccc ctcttgcgtg
     4021 ttatggcaga agcgcctaca acagaagaag tagactacta tgttgatacc atcacagatg
     4081 tagttcgtgc tgaaattggg attgactaaa tcctagaaaa ctagatgaaa ataaaaaatt
     4141 atggtacaat agcttataat attgtagccg atggagaaag acatgaatct taaacgtgaa
     4201 caagaatttg ttagtcagta tcattttgat gcgcgtaatt ttgaatggga aaatgaaaat
     4261 ggagctcctg aaaccaaggt ggatgtgaat ttccaattgc tccaacatga ccaagaaaat
     4321 caagtgactt ccttgattgt catcttgagc tttatgattg tatttgataa gtttgtcatt
     4381 agtggaacaa tctcacaagt aaaccatatc gatggccgta ttgtcaatga accaagtgaa
     4441 ttgaaccaag aagaggtgga aaccttggct cgtccatgtt tgaacatgct caatcgtttg
     4501 acttacgaag tgactgaaat tgcattagac ttaccaggaa ttaatttgga gttctaatat
     4561 gaaattagct gttttcacag attcctctgc ttatctcagt gaagagacct tgcaaagaga
     4621 agacttgttt gtcttggata ttcctgtcaa tattgatggt gaggaatatg tcgaaggcat
     4681 caatctgtct gctgaggaat tttaccaaaa aatggctcag gcttctgaat tgcctaagac
     4741 cagtcaacca agtattgcca agttagatga aatcttaact tcgctcaaag aacaaggcta
     4801 tacacatgcc ttggggcttt tcctatcttc tggaatttca ggtttttacc aaaatatcca
     4861 gtatatggtc gatgactatg agggcttaac cattgctttc ccagacactt tgattacaag
     4921 tgctccccta ggcatcatgg ttgaaagcgt ctttaattgg cgtgatcagg gcgatgattt
     4981 tgccagcatt caggataagc tagccattca aatcagccgt acgtcagcct ttatcatggt
     5041 agatgacttg gatcatttgg taaaaggtgg acgcctttca aatggggctg ccattttggg
     5101 caatttgctt agcattaagc caatccttta ttttaacgat caaggtgtga ttgaagttta
     5161 cgaaaaagtt cgtactgaaa agaaggccac caaacgctta attgaaatta tcaaggaaac
     5221 aacggcttca ggtcaatacc gggtcattgt cattcatggg aatgctcctg aaaaggctga
     5281 agaattgcgt cagcatttgc ttgattttgg cttgggttcg gatgtttcac ttgctacatt
     5341 tggtagtgtc attggaacgc acctaggagc aggaagtatt gctctaggtt atattccagt
     5401 gatttagttg gcacttctag gctagttaat cttagaaatt gaacacggac tacctgcctc
     5461 ttgaaaaaag atgcctgtct tgcctttcgt ggaaagtcag tgccattccc tatttttcag
     5521 gggcagctaa ggtcctttgt atcttgataa ttgaacacgc ctggaaccct gtgtgaaaaa
     5581 gatagttctt ccaagaagta gacacttctt gattggaatt cctattttca ctttgtgttc
     5641 ttacggactt tgtatcttag acaggagtag agatgagtat tcgggtaatt attgccggtt
     5701 ttaagggaaa gatgggccag gctgcttgtc agatggtatt gactgatcca gacttggact
     5761 tggtggcagt tttggatcct tttgagtctg agtcagaatg gcagggtatt cctgttttca
     5821 aggataaggc tgatttagct ggttttgaag cggatgtctg ggtagatttt actactccag
     5881 ctgttgccta cgaaaataca cgttttgctc ttgaaaatgg ctttgctcca gtagttggaa
     5941 cgactggttt cacgagtgaa gaaattgcag agctaaaaga attttctcgt gcccaagact
     6001 tgggtggcct gattgcccct aactttgcct tgggtgctgt cttactcatg caatttgcga
     6061 cgcaggctgc caaatatttc ccaaatgtgg agattattga gctccatcat gacaagaaaa
     6121 aggatgctcc gagtggaaca gccattaaaa cagctgagtt gatggcagag gttcgagagt
     6181 ccattcagca aggcgcagca gatgaggaag agctgattgc tggtgctcgt ggtgctgact
     6241 ttgatggtat gcgcatccac tcagttcgtt tgccaggttt ggtagcccat caggaagtca
     6301 tctttggcaa tcagggagaa gggttgaccc tccgtcatga ctcctatgat cgcatctcct
     6361 tcatgacagg agtcaatttg ggaattaaag aagttgtcaa gcgtcatgag cttgtctatg
     6421 gattagaaca cttattatga gattaacgca aatgccttct gaatttcaga aggctttacc
     6481 agtattagaa aaaattaaag aagcaggctt tgaggcttat tttgttgggg gctctgttcg
     6541 agatgccctt ctccataacc ctatccacga tgtggatatt gcgacgtctt cttatccaga
     6601 agagaccaag cagatttttc cgcgaacagc cgatatcgga atcgagcatg gaaccgtctt
     6661 ggtcttagat ggggatgagg agtatgaggt aacaaccttt cggacagagg atgtctatgt
     6721 ggactatcgc agacccagtg cggtttcctt tgtgcgctcg ctagaagaag acctcaaacg
     6781 ccgtgatttc acagtcaacg cctttgcctt ggatgagaca ggagaaatcg ttgacttgtt
     6841 ccatggttta gaagatttgg aaaagcaagt cttgcgagca gttggagtgg ctagtgagcg
     6901 tttcaacgaa gatgctttac ggattatgcg tggtttccgt tttcaggcta gtcttggttt
     6961 tgcacttgaa ccagaaacat ttaaagctat gaagaccttg acgccgcttt tggagaaaat
     7021 ttctgtagag cgtaccttcg tcgagtttga taaactcttg ctggctccat tttggagaag
     7081 gggcttggct tccatgattg agagtcaagc ttatgactat ctccctgata tggcatctag
     7141 ccaggacaag ctcaacagac tgtttgattt ggagactgat tttacttttg aatcctctga
     7201 acaagcctgg gcggctttac tatgggcttt ggagattgaa aatgcgcagt catttttgaa
     7261 atcttggaag acctcacgcc agtttgccaa gcaagttcag gatttgctga ttattttggc
     7321 tctgcgtgaa aatggagaat tgagcaagcg agattgttat cgctttgaca tagatttgct
     7381 tttacaggct gaaaatcttc gtcaagctca aggaaaagaa gtcaacccac aagccatcac
     7441 agaaaaatac caaagcttga ctatccatga caagaaagag attcagatta atggcggtat
     7501 tttgatcaag gaatatggct atcagccagg cccagacttg ggagagattt taacagagat
     7561 tgagtttgcc attgtcgatg gagaattgga gaataatcgt gaagccatcc atgcttacct
     7621 gagggagaaa aaatgagtga ttttatcgtt gaaaaactaa gtaaatccgt tggtgacaag
     7681 accgttttta gggatatttc ctttattatc catgacttag acagaattgg tttaatcggt
     7741 gtcaatggga ctggcaagac cacccttttg gacgtccttt ctggtgtttc tggatttgat
     7801 ggggatgtca gtcctttttc agctaaaaat gattaccaga ttggttactt gactcaggat
     7861 cctgattttg atgatagaaa gacagttttg gatacggttc tatctagtga actcaaggaa
     7921 atccagctca ttcgtgagta tgaattgatt atgctcgact atagtgagga caagcaggcg
     7981 cgtttggaac gtgtcatggc agagatggac tctctccaag cttgggaaat cgaaagtcag
     8041 gtcaagaccg ttcttagcaa attgggcatt caagacttat ctactcctgt tggggaattg
     8101 tcaggtggtc tgagaagacg ggtacagttg gcacaagtct tacttggcaa ccacgacctc
     8161 ttgcttttgg atgagccgac caaccatctg gatattgcga ttattgagtg gctgaccctc
     8221 tttttgaaaa attctaagaa gaccgtcctt tttatcactc acgatcgtta tttcttagac
     8281 gctttgtcaa cacggatttt cgagttggat cgtgcaggct tgaccgagta ccagggaaat
     8341 taccaggact atgttcgcct aaaggcggaa caggatgagc gcgacgcggc tcttcttcac
     8401 aaaaaagaac aactctacaa acaagaattg gcctggatgc gcagacaacc gcaggcgcgt
     8461 gcgaccaagc agcaggcccg tatcaatcga ttccatgatc tgaaaaagga agtttcaggc
     8521 agtagtgctg agacagactt gaccatgaac tttgaaacca gtcggattgg gaagaaagtc
     8581 atcgagtttc aggctgtttc ctttgcctat gaaaacaagc ctattttgca agattttaat
     8641 ctcttggtgc aggccaaaga ccgtatcgga atcgttgggg acaatggtgt tggaaaatca
     8701 accctgctca acttgattgc aggaagtctt gagccaacta aaggacaagt tgtgattggg
     8761 gaaactgttc gcatcgccta tttctctcag caaattgagg gtttggatga aagcaagaga
     8821 gttatcaatt acctgcagga agtggcagag gaggtcaaga ccagtggcgg ttctacgact
     8881 tcgattgcag agttgctgga gcagttcctt ttcccacgtt cgacgcatgg aaccttgatt
     8941 gagaaattgt cagggggtga gaaaaaacgt ctttatctcc tcaaactgct tttggaaaaa
     9001 ccaaatgttc ttcttttaga cgagccaacc aatgacctag atattgcaac tttgacagtc
     9061 ttagagaatt tcttgcaagg ttttgcaggt cccgttttaa cagtcagtca cgaccgctat
     9121 ttcttggata aggtagcgac caagattctc gcttttgagg atggcaagat tcgtcctttc
     9181 tttggtcatt acaccgacta tcttgatgaa aaagcttttg aaacagatat ggtcaatcaa
     9241 gtgcaaaagg tcgaaaagga aaaagtggtc aaggttcgag aagacaagaa acgcatgacc
     9301 taccaagaaa agcaggagtg ggcaagtatt gaaggtgata ttgaaacctt ggaaaaacgt
     9361 atcgctgcta ttgaagagga aatgcaggct aacggctctg actttggtaa gctggctact
     9421 ctccaaaaag aattggatga gaaaaatgaa gcactccttg aaaaatacga acgctatgag
     9481 tatctcagtg aatttgatag ttaagactat tcgaaaatca aagtgcaaac tatgtcggct
     9541 tcacattgcc gactcaagta cagcctgtga ccggcttcct aatttgcact ttgattttta
     9601 ttgagtataa acgaatagaa aaatcccgtc tcatgaaagg atttctctat tcttttttgg
     9661 tttcttgatg aaagatattt tgccaggttt cgtggcgacg ccagatactg gtatggatga
     9721 taccatctaa ctcatagcag ataagtttgg ttttttgact gatggaagta atttgaatgt
     9781 ccttgatagc agaatttaac tcttttttgg tcttataagc ctctttatcc atctgctctc
     9841 catcttggcg aatatagaca aaatcatcag tcaagagttc ttctagttgg tttccttggt
     9901 cgatcaattg ttcacgcata agcaattttt tatagacatt gtctaaaatt tcatcctcag
     9961 ggataggtgc tggttcgata tggacatcgg tatcaaagac gccaaaacga ttctccagca
    10021 tagactcgac ctgatccgcg atttcatggc tttcaaaaac agacaagtca ggattcatct
    10081 ctagtgtaat atccaggtag atgttgctac cgtaggtgcg acctctttgc gatttgacct
    10141 tgctgatttt gggaatttcc atgatagcct tttggtagtc ctcgagcagg cggtcgtcaa
    10201 agccatctga aagactaaag gaagactcga tgaagatatc ataggcagtc ttcaagataa
    10261 agaaagtgat gatgatagca accagtttat ccacaatcgg ataattgaaa ctactagcta
    10321 ggatggcaat ggcggttcca agtgaggtaa cagcgtcaga aagattgtcc ttagcagctg
    10381 ccttcagcgc cttggagttg gatttcttac tgaggcgagt attgtagaga tagaccacaa
    10441 acataatcgc tgcagaaatg attcctagag ttgcaccaag aggatcaatg accgtttctt
    10501 cccgactgag aatcttttga atggtatctc ttagaacatc gaaaccgaca tagaacatga
    10561 tgatagaagt gatcaagctt gccaaatctt caatcttcca atgaccaaaa cggtggtcac
    10621 ggtctgcagg ctggcgcgcc atccgaatcc cgattaagag ggccacattt ccaatgatgt
    10681 ccgatacgtt attaaaacca tcggccacca aactggatga atgaaggaga tgaccagctg
    10741 ctaatttggc tgcagacaag atcaaatagg tcgaaatact gataatggct ccacgctcag
    10801 ctaacttgag atttgagata gattgcttca ttcaacttcc tttctagtca catagcattc
    10861 taccattata ctggttttca aggaaaaatt caaataaggc agtttttact ctttgaaaac
    10921 tccccaaaat ttggtataat ggtactactc aagggagtag ctggcagaaa cctgtgatag
    10981 tgtcgtcatt ccgaatttta tgctgaaaag tatgctttcc ggccctatct taaacagcga
    11041 gacttgttat gattaacagg tctctttttg tttgtcgtga aaagatatga cagaaagaga
    11101 gtctgttttg cacacaatgt caaggaggag acacatgtca aaagaacaaa aacgccaagc
    11161 gttttatact cagagccctg aagaggtctt gcaggctgtg gatgcgaccg agcaaggttt
    11221 gtcatcaagt gaggcggaaa agcgccttgc cgaatttggg cacaatgaac tcgaagaagg
    11281 cgagaaacga tcaatcttgg tcaaattcat cgagcaattt aaggatttga tgattatcat
    11341 cctagttgcg gcagcaatct tgtcagtcgt gacttctggt ggggaagata tcgcagatgc
    11401 cattatcatc ctagctgtgg ttatcatcaa cgctgccttt ggtgtttacc aagaaggaaa
    11461 agctgaagaa gctattgaag ccctcaaatc catgtctagt ccagttgccc gcgttcttcg
    11521 tgatggacac atggcagaga ttgactctaa agaattggta ccaggcgata tcgttgccct
    11581 tgaagcaggt gacgtggtac cagcggacct acgtttgata gaagccaact ctcttaaaat
    11641 tgaagaagca gccttgacag gtgaatctgt accagtcgaa aaagacttgt cagtcgagct
    11701 tgcgacagat gctggtattg gtgaccgtgt caacatggcc ttccaaaact caaacgtaac
    11761 ctatggtcgt gggatgggtg ttgttgtcaa tacaggtatg tacactgaag ttggtcatat
    11821 tgctggtatg cttcaagatg cggatgagac tgatacacca ctcaaacaaa atttgaacaa
    11881 cctttctaag gtcttgacct atgctatctt ggtcattgcc cttgttactt ttgtagtggg
    11941 tgtcttcatt caagggaaaa atccacttgg tgagttgttg acttctgttg cccttgccgt
    12001 tgcagccatt ccagaaggac ttcctgctat cgttaccatc gttctttctc ttggtactca
    12061 agttttggcc aaacgacatt ctatcgttcg taagttgcca gcagttgaaa cacttggttc
    12121 aactgaaatc atcgcttctg ataagactgg tacgctgact atgaacaaga tgacagtcga
    12181 aaaagtcttc tacgatgcgg ttctacatga ctcagctgat gatattgaac taggtcttga
    12241 aatgccacta cttcgttcag ttgtcttggc caatgatacg aaaatcgatg tggaaggtaa
    12301 cttgattggt gacccaaccg aaacagcctt tatccaatat gccttggaca agggctatga
    12361 tgtcaaaggt ttcttagaga aatatcctcg tgtagctgaa ttgccatttg actctgaccg
    12421 taagctcatg tcaacagttc acccattgcc agatggtcgt ttccttgtag cagtcaaggg
    12481 tgcgccagac caactcttaa aacgttgtct tcttcgtgat aaggctgggg atattgctcc
    12541 gattgatgag aaggttacaa atctcattca tacaaacaac tctgaaatgg ctcatcaagc
    12601 cttgcgtgtc cttgcaggtg cttataagat tattgacagc attccagaaa atctcacttc
    12661 tgaagagctt gaaaatgatt taatttttac tggtttgatt gggatgattg accctgaacg
    12721 tcctgaagcc gctgaggctg ttcgtgtggc taaggaagcg ggaatccgtc caattatgat
    12781 tacaggtgac caccaagaca cagcggaagc cattgccaaa cgtttgggaa tcattgacgc
    12841 aaacgataca gaaggtcacg ttttaactgg tgctgaactc aatgaactgt cagatgaaga
    12901 atttgaaaaa gttgttggtc aatactctgt ttatgcccgt gtgtctccag aacacaaggt
    12961 tcgtatcgtc aaggcttggc aaaaacaagg taaagtcgtt gccatgacag gtgacggtgt
    13021 caatgacgcg ccagctctga aaacagccga tatcggtatc ggtatgggaa tcactggtac
    13081 agaggtttct aagggggctt ctgatatgat tcttgcagat gataactttg cgactattat
    13141 cgtcgcagtg gaagaaggac gtaaggtctt ctcaaacatt caaaagacta ttcagtacct
    13201 actttctgct aatactgctg aagtattaac catcttccta tcaaccttgt ttggttggga
    13261 tgtcttacag ccggttcatc ttttgtggat caacttggta acggatacct tcccagctat
    13321 cgctcttggt gttgaacctg cggaacctgg tgtcatgaat cataaaccac gtggacgcaa
    13381 ggcaagcttc ttctcaggtg gtgttttgag ttctatcatt tatcaaggtg tactccaagc
    13441 agctcttgtt atgagtgttt atggccttgc gattgcttac ccagttcatg tgggtgacaa
    13501 tcatgctatt catgcagatg ccctaacgat ggcctttgca acccttggtt tgattcagct
    13561 cttccatgcc tacaatgtca agtctgtcta ccaatccatc ttgacagttg gcccattcaa
    13621 gtctaagacc tttaactggt ccatcttggt atcctttatc cttctcatgg caacaatcgt
    13681 tgtagaaccg cttgaaggaa tcttccacgt aaccaaacta gacttgtcac aatggggaat
    13741 tgttatggct ggaagcttct caatgattat catcgtcgaa atcgttaagt ttattcaacg
    13801 caaactcggt tttgacaaga atgcgattta agggaggtct cggatgtcag catatcaatt
    13861 accgaccgta tggcaggatg aagctagtaa tcaaggagct tttacggggc taaacagacc
    13921 aacagcaggt gcccgtttcg aacaaaactt gccaaaagga gaacaagctt ttcagcttta
    13981 ttcactggga acaccaaatg gtgtgaaggt tactatctta ttggaagaat tactagaagc
    14041 tggttttaag gaagcggctt acgacttgta taagattgct atcatggatg gggatcaatt
    14101 cggatcagac tttgtgaagc tcaatccaaa ttccaagatt ccagccttat tggaccagtc
    14161 aggtactgaa gatgtaagag tctttgagtc tgctcatatt cttctttacc ttgctgagaa
    14221 atttggagcc tttttaccaa gtaatcctgt ggaaaaggta gaagttttga attggctatt
    14281 ctggcaagca ggtgcagcac cttttctagg tgggggattt ggacatttct tcaattatgc
    14341 tcctgaaaaa ttggaatatc ctattaaccg ttttacgatg gaagtgaaac gccagttgga
    14401 tttattggat aaggaattgg ctcagaaacc ttatattgca ggcaatgact atacgattgc
    14461 agatattgct atctggtctt ggtatggaca gttagttcaa ggaaatcttt accaaggttc
    14521 tgcaaaattc ttggatgcct caagttatca aaatctagta aaatgggcag aaaaaattgc
    14581 caatcgtcca gctgttaagc gtggcttgga agtaacttat acagaaatta aatagcaaaa
    14641 aagtcatctt cggatggctt tttgctacag ttgagggaaa gggtttgcag ttatcggctt
    14701 ttgtgctata attgctataa tatagtaaaa agcaagagga gtgtgaggaa gtggaaaaga
    14761 aaattgtgaa ggatatctta tttttatctc aagtgtctca gccggcaagt caggaggacc
    14821 tttatcttgc cagagatttg caggatacac tcttagcaaa tcgtgatacc tgtgttggtc
    14881 tagctgccaa tatgattggg gtgcagaagc gcgtgattat ctttaatctt ggcttggttc
    14941 ccgtggtcat gtttaaccca gtgcttctgt cctttgaagg atcctatgag gcagaagaag
    15001 gctgtttgtc cttggtaggt gtgagatcaa ctaagcgtta tgaaaccaca aggcttgcct
    15061 atcgtgacag caagtggcag gaacagacca ttaccttgac aggcttccca gctcagattt
    15121 gccagcatga gctggatcac ttggaaggac gaatcattta ggaggaaagc aaatgaaacg
    15181 aatagtctgt gaacttattt ttatcgcaac gacctggtat atctttttac cgccccttaa
    15241 cctgaccagc tgggaatttc tcttcttcct ctgtgggcat ttgttagttg tggcaatctt
    15301 atttggcttt ggcaagggga taaaccttgt caaaacggtt catgtgcgcc acggtaaggc
    15361 ggaagctgcc ttaaatcttg agggtttcaa aatcaatcgg ttagggaaaa ttctgttagc
    15421 ttcgattgga ggaattcttc tcttggcagc tttggtttcc ttggtaactt ccagcatgtt
    15481 tcaggctaaa aattatgcca atgtagtcac ggttacggaa aaagacttta ctgaatttcc
    15541 taggagtgac accagtaagg ttcctatcct agatagaagt actgctgaaa aaattggaga
    15601 ccgctacttg ggttccctaa ccgataaggt gtcgcaatac gtagcggcag atacctatac
    15661 ccaattgaca attgatggga aaccttatcg ggtcacacca ctagaatatg cagaccctat
    15721 caaatggttt aacaatcaag ccaagggaat cggtgagtat atcaaggtgg acatggtaac
    15781 tggaaatgcg gatttggtgg acttgaagac accaatcaag tattcagact cggagtattt
    15841 taaccgtgat gtcaaacgtc acctgcgctt gaagtacccg actaaaatct ttaaaactcc
    15901 atcttttgag gtggacgatg agggcaatcc tttctatgta gcaacggttt accaaaagca
    15961 atttggactt gcggttcctc gtcctgcttc agtcattatc ttggatgcta caaatggaga
    16021 aaccaaggaa tacagcttat cagatgttcc agaatgggtg gacaggatct atccagcaga
    16081 ggaaaccatt gagcaaatca actacaacgg caagtacaag gacggtttct tgaatgccat
    16141 gatttccaag aaaaacgtga cccagactac caatggctat aattacttgt ctatcggtaa
    16201 tgacatctat ctctacacag gtgtgacgtc ggctaatgcg gatgagagta atcttggttt
    16261 catccttgaa aatatgcgaa caggagaaat tactaagtat agcttggctt ctgcgacaga
    16321 agaatcagcc cgtgaatcag cagaaggtgc tgttcaggag aaatcctaca aagcaacctt
    16381 cccaatcctc atcaacctca atgacaagcc tctctacatc atgggcttaa aggacaatgc
    16441 tggcttggtc aaagagtacg ccctggtaga cgcagtcgag taccaaaatg ttatcgttgc
    16501 tactacagtg gaagagatgc tcagcaagta tgccaataaa aacgaccttg aaattgacaa
    16561 tgcaacgaca gaaagcatca agggagtagt agcagacctc aaatcagctg ttatcaaggg
    16621 agacactgtc tacttcttta aagttgatgg caagatatac aaggtcaagg cttcagtatc
    16681 cgatgacctt ccttaccttg aaaatggtaa aaccttcgaa ggtcaagtag gaaaagacaa
    16741 ttatctcaag acctttaaag tacaataata ctcttcgaaa atctcttcaa actacgtcag
    16801 ctctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagtttc ctagtttgct
    16861 ctttgatttt cattgagtat aaaaaaggtt tatttaagaa atatatgtta taataaggta
    16921 aattaagcct aatggaggac aaagaaatgg gtttttattt gatggttgct tcaatgctac
    16981 tcggactgct cgctttgaag ataggttttt ctcagttcaa ggagaaaaaa gataaattct
    17041 tatctatctt aacaagttta gctggcatag cccttgttct cgcggctgtc tggttaggat
    17101 ggcccaaata aatagaaaaa cctcggtcaa accgaggttt ttcaaatgaa tttcttggtt
    17161 gtggctaaaa aatatgctac actatcaata tgaaaatttt aatcccaaca gcaaaagaaa
    17221 tgaacacaga cttgccaagt atcgaggcaa ttcctttaaa accagaaagt cagaccgtgc
    17281 ttgatgcctt ggctctctat tctgccagtc aattggagag tttctacaag gtatcagctg
    17341 agaaagcggc ggaagaattt caaaatatcc aagctttgaa aaggcaaact gctcaacact
    17401 atccagcctt gaaacttttt gatgggctta tgtaccgcaa cattaagaga gataagctga
    17461 ccgaggcgga acaagattat cttgaaaatc atgtcttcat tacctcggct ttgtacggtg
    17521 ttgttccagc cttgtcaccc atggctcctc accgtttgga ttttttgatg aaattaaaag
    17581 tcgctggtaa gactttgaag agccattgga aggcagtcta tgatgaagct ctgaagaagg
    17641 aagaagtgat tttctctctc ttgtcatcag agtttgagac tgtattttct aaggaaatca
    17701 gagcaaagat ggtgaccttc aaattcatgg aggatagagg cggtcagctg aagattcact
    17761 caactatctc caagaaagcg cgcggggcct ttctaacagc tttaatagaa aatcaagtac
    17821 aaactgtggg ggaagcacgt cgcttgaact ttgctggatt tgtttaccga gaagatttgt
    17881 cacaaccaca gggattggtt tttgtaaagg aagtatagaa atcagataag agaaaagtca
    17941 gcacgattat gctgactttt ttgataactt ataaagtatt ggacaaatga atctcatttg
    18001 ataaggtgtt aagatgatta accaaattca tgattgacag atgatttcgg caaataaact
    18061 tcttggtaat ccctggaata tagaaattag acacgatgat atcgtaatct gtctgattta
    18121 aaatctcagg acttgttttt aattcatccc aaatatcgaa ggtaaagcgg ttattacagt
    18181 aataggaaag catatcaaca aaggtaaggg atatagcatg gtcaaagtta ctgataatca
    18241 aaaccttgat aggtggtcga ttttctaaca actgagtaga gaggttttca gcatgggtta
    18301 agatggtgta catcaagtgt tccaactgtt caggatggtc atgctgtcca attgcctgac
    18361 ggtattgagc caattcttga cgcgcacttc ccataaagtc tgggaagtaa acttcaaatt
    18421 ttttaatcgt caatgctttt tgttcaaata agattggggt agaaaagatt tcctgtcttt
    18481 caaagaaagc agtgttgtgt aaatgccaaa tcagctcatc gtggttagta aaagtaatct
    18541 tatattgttt tgataggcct tctaatattt gactaagtaa ttggtaagag tatcttgctt
    18601 gactgttatc ttctaaagag ttgaagaatt cttgaggatc taggaaaata tcattctgaa
    18661 gaaaagaaat aaaaatttga gcgattgtat ctggagtcat ttcaagccca aaatgtttag
    18721 agaaataagc aagtgtctcc tggaaattcg gaatgctctt taaaagagga taatattctt
    18781 tgtaaaagtg gttggggagg tcaatgaagt gaccattttt aaccctgtgg atactgatag
    18841 ctatcatcag tttgtacatt ctataaattg aaaatcgcat tggataattt gtgattttat
    18901 agaagaagtc agcaaactct gttaaatctt cctcaggaag gtcaggaaaa ggccaatcta
    18961 ggaaataata gcgctcagaa aagtattggg caaaaaagta acgaatatcg atctcatttc
    19021 cacgtatctc ggatggagta aaagataatt caatttgaaa ctgagatgaa agaacctttg
    19081 taatatttct gcccagtcga tagaggttgg cactactaaa gtaaagattt tctattgttc
    19141 gataaatagg tagcccctca ttgaagaaca tatactctag taattgaaaa gattgagaat
    19201 tagcaagaaa gtattgataa atatcttcta cactagtgtt aacttctaaa tctatcatga
    19261 ttccgttaac agaagattgg atgttaatag atggaaaggc aatacgtaat tcatttaaat
    19321 cactttttaa gattcgttct gtacatccta atttttcggc tagttcatgt aatttcatcc
    19381 aattacgatt ctgaattagg gtttccatta agcgtaattg tctttgaact tttgtggata
    19441 aaaggtttct catacgggta tttcctccat aattcattat atcttttttt gtctaagtag
    19501 tataaaaaaa ttagagcaag aaaattgtcg
//
LOCUS       DAVMHG010000027        19350 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMHG010000027 DAVMHG010000000
VERSION     DAVMHG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.027"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            436..951
                     /locus_tag="VYR08_000750"
     CDS             436..951
                     /locus_tag="VYR08_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3466317.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSLANTTQ
                     TTPVIFSAVTDPVSAKLVESREHPGGNVTGTSDQSSDAISTQINLIKKVLPKAKTIGI
                     LYTQSEPNSVV"
     gene            973..1374
                     /locus_tag="VYR08_000751"
     CDS             973..1374
                     /locus_tag="VYR08_000751"
                     /inference="COORDINATES: protein motif:HMM:NF016292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466318.1"
                     /translation="MKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISST
                     METVKQVSIKHKVPVFGGSTEMIAVGDLYNYGTNYEELGRQTARMLIRVLKGEKPENI
                     AVELPEKLELHTNQEMADALGIDISKLEGKE"
     gene            1386..2263
                     /locus_tag="VYR08_000752"
                     /pseudo
     CDS             1386..2263
                     /locus_tag="VYR08_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2276..3079
                     /locus_tag="VYR08_000753"
     CDS             2276..3079
                     /locus_tag="VYR08_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3466319.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3112..3822)
                     /gene="treR"
                     /locus_tag="VYR08_000754"
     CDS             complement(3112..3822)
                     /gene="treR"
                     /locus_tag="VYR08_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV3466320.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            4007..5974
                     /gene="treP"
                     /locus_tag="VYR08_000755"
     CDS             4007..5974
                     /gene="treP"
                     /locus_tag="VYR08_000755"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV3466321.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            6152..7777
                     /gene="treC"
                     /locus_tag="VYR08_000756"
     CDS             6152..7777
                     /gene="treC"
                     /locus_tag="VYR08_000756"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV3466322.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     LVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            8033..8281
                     /locus_tag="VYR08_000757"
     CDS             8033..8281
                     /locus_tag="VYR08_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV3466323.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            8488..9282
                     /gene="racE"
                     /locus_tag="VYR08_000758"
     CDS             8488..9282
                     /gene="racE"
                     /locus_tag="VYR08_000758"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV3466324.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEKIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            9279..10250
                     /locus_tag="VYR08_000759"
     CDS             9279..10250
                     /locus_tag="VYR08_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV3466325.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            10226..10747
                     /locus_tag="VYR08_000760"
     CDS             10226..10747
                     /locus_tag="VYR08_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV3466326.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            10744..11205
                     /locus_tag="VYR08_000761"
     CDS             10744..11205
                     /locus_tag="VYR08_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3466327.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            11196..11936
                     /gene="xerD"
                     /locus_tag="VYR08_000762"
     CDS             11196..11936
                     /gene="xerD"
                     /locus_tag="VYR08_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV3466328.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            11936..12664
                     /locus_tag="VYR08_000763"
     CDS             11936..12664
                     /locus_tag="VYR08_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV3466329.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            12683..13252
                     /locus_tag="VYR08_000764"
     CDS             12683..13252
                     /locus_tag="VYR08_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV3466330.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            13239..13961
                     /locus_tag="VYR08_000765"
     CDS             13239..13961
                     /locus_tag="VYR08_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV3466331.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            13961..14203
                     /gene="yidD"
                     /locus_tag="VYR08_000766"
     CDS             13961..14203
                     /gene="yidD"
                     /locus_tag="VYR08_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV3466332.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(14428..15393)
                     /locus_tag="VYR08_000767"
     CDS             complement(14428..15393)
                     /locus_tag="VYR08_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466333.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEIATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(15489..16241)
                     /locus_tag="VYR08_000768"
     CDS             complement(15489..16241)
                     /locus_tag="VYR08_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3466334.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(16238..17194)
                     /locus_tag="VYR08_000769"
     CDS             complement(16238..17194)
                     /locus_tag="VYR08_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV3466335.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(17184..18143)
                     /locus_tag="VYR08_000770"
     CDS             complement(17184..18143)
                     /locus_tag="VYR08_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466336.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLEVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(18566..19267)
                     /gene="galE"
                     /locus_tag="VYR08_000771"
     CDS             complement(18566..19267)
                     /gene="galE"
                     /locus_tag="VYR08_000771"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3466337.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHYAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPATEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         5507 a         3754 c         4504 g         5585 t
ORIGIN      
        1 ttggactgat ttgaaaaact ttgaaagctt cctgaaagaa ttttcgaaaa attttaaaaa
       61 attttgaaat attattgaca ggtgatgaaa aaggtggtag gataggaagg atcaaaaaag
      121 aaagcagaaa aagaaatgaa taagagacaa gccgtaacta tgaatcaaaa cttttacttt
      181 agctacttta gatagtgttt gtagacatgt caccatggat gcaaacagtt caagcaattt
      241 gtttgtatct atgtggctaa gatttttgat tgtggtccat atagatgaat atctaaatgg
      301 actagctaag ttgtaacttg ttaggttatt tcaagtaccc gtttagatag tatctaggcg
      361 gttttttgtt ttatcttttc tgagaaggag tttcattatg aaagttgttc gaaaatcact
      421 agccccctct tggtagtggg gatcctcttg acttctctga tcagtttgca tcagttgaag
      481 gcagataaga aaagagatgt ctttcgtatc ggtatttcgc agtttattac ccaccagtcc
      541 ttagacgcta ctagagaagg atttgtggat gagctggcca aacaaggcta tgttgagggg
      601 gaaaatattg agattgattt gcaaaatgca cagggagaac aaagaaatct aaaaacgatt
      661 tcccagcaac tagcagaatc cagcgatgtc gttctagcca tcgcaaggcc ttctgctcag
      721 agcttggcca atacgacgca aacgacacca gttatctttt cagctgtaac agacccagtc
      781 agtgccaagt tggttgagtc aagagaacat cctgggggca atgtaactgg gacaagcgat
      841 cagtcatcag atgccatttc aacccaaatc aatttgataa agaaagtgtt gccaaaggct
      901 aaaacgattg gaatcctcta tactcagagc gagcccaatt cggttgttta aaatgatgaa
      961 gctaagcgac ttctgaaaga aaaaggcttt accgttgttg aaaaaacaat cttggacagt
     1021 aataacgtca aggcggcagc agagagtttg atggcagagg tggatatggt ttttgtccca
     1081 actgataata tcatttcgtc aaccatggaa acagttaagc aggtttctat taaacacaag
     1141 gttccagtat tcggtggttc aacagaaatg attgctgttg gtgacttgta taactacgga
     1201 accaattatg aagaattggg aagacagaca gctcgcatgc tgattcgtgt tttaaaaggt
     1261 gagaaaccag aaaatatagc agttgagttg cctgaaaaac tggaattgca taccaatcaa
     1321 gaaatggcag atgcattggg aattgatatt agtaagttag aaggcaagga ataaggagga
     1381 ttaagatgaa ttttgtgtta tctagtttat cagaaggttt gttgtggtcg attgtggcga
     1441 ttggggtcta cttgactttc cgtattttgg atattgcgga tataactgct gagggagcct
     1501 ttcctctggg ggcagcagtc gttgtatcac agatacaggc aggggcaaat ccttggcttg
     1561 cgactttact tgctttgctg gcaggtatgg tagcaggtct tgtatcagga atgctccaca
     1621 ccaagatgaa aatcccagct ctcttgacag ggattgtgac cttgacaggg ctctattcaa
     1681 tcaatattaa aattatgggc agtgtaccca atctttcctt gggagattct gcaaccgtct
     1741 ttaaacagtt ggcgagcttg ggtctgacaa atgaaggagc tgttttctca cttagtttag
     1801 tctgtctctt acttgtttgg tcttgaccct tttgatgaaa acagagattg gtttggtctt
     1861 gcgttcgact ggggataata tcccgataag tgaggccaat ggagtcaatg tagacaccat
     1921 gaagattgtt ggttacatga tttcaaacgg tttgattgcc ctatgtggtt ccttgtttgc
     1981 ccaaaatgat ggattttcag atgtgacttc tgggacagga accattgttg ttggtttgag
     2041 ctcggaagtc ttgatacacg acttgaccat tggaggccgc ttattatcca tcggaatcgg
     2101 tgctattgtt taccgtttga ttattttaaa tatctatgaa attccaaatc tagatcaaaa
     2161 cttggtacgt ctctttaatg caatcttgct cgctttggtt ttatttgcac cagaattgca
     2221 aaagagatta aagattcgtg gtctgaaatt gagaaatgaa taggaggaga aagttatggc
     2281 aagtttgtta acacttgaaa atattcacaa gacatttgaa gcagggacgg tcaatgagaa
     2341 ccatgtcctc aaaggattag atttagaggt tgaagaggga gattttatct atgtgattgg
     2401 tggaaatgga gcaggaaaat ccactttgat gaatatcttg gctggcaatc tatcggtgga
     2461 cgaaggggac cttctactgg cagggaaatc cattaaaaat ctgagtgtca gaaagagagc
     2521 caaggatatt gcccgtgttt ttcaatatcc taagatggga acagcttctc gtttgacgat
     2581 tgaggagaat atggctattg ccttgagacg tgggcaaaag agagggcttg gttggggtgt
     2641 gaaggagaag gatagaatcc agttccaaga ggccttgaag gagttgaata ttggtcttga
     2701 aaaccgctta aaagtagata ctcaatatct ctcaggagga caaagacagg ctttgaccct
     2761 agtcatggca gctctggtga aacccaaact tttacttttg gatgaacata ctgcggcact
     2821 tgacccgaaa accagtcaaa tggtgatgga cttgacgcaa aagattgtgg agcaccatca
     2881 gtggacgact ttgatgatta cccatgatat gaatcatgcg attgagtacg gcaatcgtct
     2941 cattatgctc tatcaaggca agatagtggt ggatgtcaag ggagaagaga aaaagcatct
     3001 gacagttgaa gaccttatgc atctcttcca gaaaaatagt ggccaaagtc tggtcagtga
     3061 tgaattagtt ttgggataaa gaaaaaggct gagatctggt gtctcagtct tttatttctt
     3121 gcgttttgca aaatctacaa atctaaattt ttctaactta tggcgacttt cggtaaactg
     3181 aaattggcgt ccgtcttgga gatagacttt tgatttaatc gaaacgacat aagggtcttt
     3241 gccaatgtcc atgagaatct tgtctcggtc gcttgagtgg tcaatggtga tttccttctg
     3301 agcataatca ataaggagtt tgaggccatt ttctatataa gaatagatag actgctcagc
     3361 aatttggcga gtgagatttg ggatgagttc catatccaga tagtccgtat ccagaacgga
     3421 taccagatca tccaccacac gctggcggac caccttccaa accatccgaa actctgggaa
     3481 accggttatc agtgaggatt ttttatcaat aataatcttg tccagactga ccacgttggt
     3541 tttagagcgc agtccaagtt ctttaactag ttcttggtag ctggttaggt tggatacagg
     3601 gaaattgacg gtttcttctt tgacgacttg agaaccttgc cctcttatct ttttgatcaa
     3661 tccttcctct tggaggagag acagggcctt tcggacggta tcacgactca cttgatattg
     3721 ctccataagg tcgtgctcgc taggaaggaa atctccgaca gcgtaagtct cgttttgaat
     3781 ggtttcttgg atttgcttaa ataattgttg gtatttcttc attttgcctg ctttaatagg
     3841 tttttagtct agtttaatga acacctgtat ctattttacc atacaagtgc tagaaaattt
     3901 acaaaaaaac acccttttta taccaaacaa gttgcagaca agtttggtat cgtttacaat
     3961 atacttatca aatcaaactt gctttgacaa gtataaggag aatcaaatgg gaaaatttga
     4021 acaagaagcc aaagatctgc ttcaggcaat tggaggcaaa gaaaatgtga ctgccgtaac
     4081 tcactgtgcg acacggatgc gctttgtttt aggagatgat aagaaggcta atgttaaagc
     4141 tatcgagtca attccagctg ttaaaggaac ttttacaaat gcaggtcaat ttcaggtaat
     4201 cattggaaat gacgtgccca tcttttataa tgattttaca gccgtttcag gtattgaggg
     4261 tgtttccaaa gaagcagcca agtctgcagc taagagtaat caaaacgtgg tccaacgtgt
     4321 tatgaccact ctggcggaga tttttactcc gattattcca gccttgatag tcggaggatt
     4381 gatcctcggt ttccgtaatg tcttggaagg tgtccattgg tcgatgttgg atggcaagac
     4441 catcacagaa tcctctcagt tttgggcagg tgtcaatcac ttcctctggt tgcctggtga
     4501 agctatcttc cagttcttac cagtagggat tacttggtct gtttctcgta agatgggaac
     4561 cagccaaatt ttgggaattg ttctcggaat ctgtttggta tcgcctcagt tgctcaatgc
     4621 ctatgcggtt gcttcaacgc cagcagctga tatcgcggca aactgggttt ggaattttgg
     4681 ctattttact gttaatcgta tcggttacca agcccaagtt atcccagcct tgcttgcagg
     4741 tttgagtctg tcttatcttg aaatcttctg gcgcaagcat atcccagaag tcatttctat
     4801 gatttttgta cctttcttgt cattgattcc agccttgatt ttggctcata ctgttttggg
     4861 accaatcggt tggacaattg gacaaggact ttcatcagtt gtcttggcag gtttaactgg
     4921 tccagttaaa gggctcttcg gtgcaatttt tggtgccctc tacgctccat ttgtcatcac
     4981 aggtctgcac catatgacca atgccattga tacacaattg attgcggatg ctggtggcac
     5041 tgccctatgg ccaatgattg ctctttctaa tattgctcaa ggctcagccg tgtttgccta
     5101 ttatttcatg catcgccatg atgagcgtga ggctcaggtt tcacttcctg caaccatttc
     5161 agcctatctc ggtgttacag aaccagctct ttttggggtt aacgtaaaat atatttatcc
     5221 atttgttgct gggatgactg gttcagccct tgcaggcatg ttatccgtta cttttaatgt
     5281 aactgcggct tctattggta tcggtggttt gccaggtatt ctctctattc aacctcaata
     5341 catgctgcca tttgcaggaa ctatgctagt tgcgattgtt gttccaatgc tcttgacttt
     5401 cttctttcgc aaggctggtc tcttcacaaa aatagagggc gatacgaact tgcaggcaga
     5461 attcgttgct caagaagaag cagaatttgt gaaccatgaa ccagtagaac ttacttcggt
     5521 agaaattatc agcccactaa ctggccaagt gaaagaattg agtcaagcga cggatcctgt
     5581 ttttgcatca ggtgtcatgg ggcaaggtct ggtcattgaa ccaagccaag gtgagttgac
     5641 ctctccagtt aatggaacag tgacggttct tttccctacc aagcatgcca tcggcattgt
     5701 ctctgacgag ggagttgaat tgctcatcca catcggtatg gatacagtag gtcttgatgg
     5761 caaaggtttt gaaagtcttg tagtccaagg agatcacgtt atagttggcc agcaactgat
     5821 tcgttttgat atggatgtca ttaaggctgc aggtctggtg acagaaactc ctgttatcat
     5881 caccaaccaa gatgcttata cagcgactat tactggaact tatccgacaa cgatccaagc
     5941 tggagcatct ctcatggtcg ctacacgaat ctaatactct tcgaaaatct cttcaaacca
     6001 cgtcagctct atctgcaacc tcaaaatagt gttttgagca acctgtggct agcttcctag
     6061 tttgttcttt gattttcatt gagtataatt gaaatagcac aaatagggac ggaagactgc
     6121 ttcctccctt ttatcagaaa aggagaaaca gatgacactt gataaaggaa aagtagtcta
     6181 tcaaatctat ccaaaatctt acaaagacac cactgaaaac ggtattgggg atttccgtgg
     6241 gattatcgaa aaaattccct atttagccaa actaggtgtg gatatggtct ggctcaatcc
     6301 attctatcca agccctcaac gggataatgg ttacgatatt tcagattata tggcagtgga
     6361 tcctcttttt ggtgatatgg ctgattttga ggaaatggtg tgtatcggta aagagcacaa
     6421 gattgacttt atgctggaca tggtacttaa tcattgttcg acagagcatg aatggtttca
     6481 gaaagcccta gctggtgata agtattatca agactttttc ttcatccaag accaaccaac
     6541 agattggcag tctaagtttg gtggttctgc atgggcgcct ttcggggata ctgggaaata
     6601 ctatcttcac ctatttgatg agacccaggc tgaccttaac tggcgcaatc ccaatgtccg
     6661 taaggagctt ttcaaggttg ttaatttctg gcgcgacaag ggtgtcaaag gttttcgatt
     6721 tgatgtgatc aatttgattg gtaaggacga ggtttcagtg gactgtcctg aaaatgaagg
     6781 gaagccagct tatacagaca aacccctcgt tcataactat ttgcgtatga tgaaccaagc
     6841 cacttttgga tctgacgata gctttatgac agttggggaa atgtcttcta ccaccatgga
     6901 aaactgtgtc ctctattcgt cgcctgatcg tcaggaatta tccatgacct ttaattttca
     6961 tcacctcaag gtggactata aggatggaca aaagtggacc ttggctccct ttgattttga
     7021 agagttgaag agtctttacc atagttgggg taaggaaatg agtgataaag acggttggag
     7081 tgccctcttt tggaacaatc acgatcaacc acgtgccttg aaccgttttg tcgatattca
     7141 aaacttccgc aaggaaggag ccaccatgct agcagccagc attcacctgt cacgtgggac
     7201 accttatatc tacatgggtg aggaaatcgg gatgattgac ccagactatg attctatggc
     7261 tgattatgtg gatgtcgaat cgcttaatgc ctatcagatg ctcttagaag aaggaaagag
     7321 tcagcaagaa gccttccaga ttattcaagc taagtcgcgt gataattcac gcattcccat
     7381 gcagtgggat gcttcggaaa atgcaggatt ttcaacaggc actccttggt tgaaagctgg
     7441 taaatcctat aaatacatca atgtagaaaa tgaaatccag ggtccgattt ttaccttcta
     7501 tcaagacttg attcgactgc gtaaggaaat gccaatcatt tcagaaggaa gttacaaacc
     7561 agcctttgaa gatagcaaac aagtctacgc ttttgaacgc cagtttgagg atcaaaagtt
     7621 actagtgctc aataactttt atgctaaaga agtggaaatc gacttgccag cagtctacca
     7681 aaatggacag attttgatat caaactatga agatgcagaa gtgtctgaga aaattctact
     7741 caaaccctat caaacactgg ctatttatgt aaattaatac tcttcgaaaa tctcttcaaa
     7801 ccgtgtcagt tttatctgca acctcaaagc ggtgctttga gcaacctgtg gctagcttcc
     7861 tagtttgctc tttgattttc attgagtata aacttagcaa ggagacctct ttttcagaaa
     7921 agcagggttc tttttgctgt ctattccatc cctgaaagtg tagaacaaac tggtcagggt
     7981 ggctagtttg tggtataatg aaagggactc aaaaagaaac aggagaaaaa tgatggattt
     8041 acttttagca attgtattga ttgtgctagc ttttctagga ggagctcttg gaggaatgta
     8101 cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac ccacgtttga atgctgaagc
     8161 agttcgtact cttttgagtg caaatggtca aaaaccaagc gaagctaagg tacaacaagt
     8221 ttaccaccaa atcatccgcc aacaaaaggc agcccttgct aacaataaaa agaaaaaata
     8281 aataaggaaa agtctgggat gaaagttcca gacttctcac tatgttggtt atggtttagg
     8341 gatgagactt tttccttgca ttctattgat atttgattat gattaagaga gaggtagttg
     8401 acattctatc aagctgtcgg tcagttcgtt cagtacaagg aatcataatg aacgatcaat
     8461 cagaaaaaag actagaaaga agactgtatg gataatcgac caattggttt tttggattcg
     8521 ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc agcttcccca tgaagaaatc
     8581 gtctatattg gagattcggc gcgggcgccc tatggccccc gtcctgctga gcaaattcgt
     8641 gaatatactt ggcagctggt caactttctc ttgactaagg atgtcaaaat gattgtcatt
     8701 gcttgtaaca ctgcgactgc ggtcgtctgg gaaaaaatca aggctcaact agatattcct
     8761 gtcttgggtg taattttgcc aggagcttcg gcagccatca agtccagtca aggtgggaaa
     8821 atcggagtga ttggaacgcc catgacggta caatcagaca tataccgtca gaaaatccat
     8881 gatctggatc ccgacttaca ggtggagagc ttggcctgtc ccaagtttgc tcccttggtt
     8941 gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg tctatgaaac cctgcgtccc
     9001 ttggttggaa aggtggatag cctgattttg ggctgtactc attatccact ccttcgccct
     9061 attatccaaa atgtgatggg gccaaaggtt cagctcatcg atagtggggc agagtgcgta
     9121 cgggatatct cagtcttact caattatttt gaaatcaatc gtggtcgcga tgctggacca
     9181 ctccatcacc gtttttacac aacagccagt agccaaagtt ttgcacaaat tggtgaagaa
     9241 tggctggaaa aagagattca tgtggagcat gtagaattat gacaaataaa atttatgaat
     9301 ataaggatga ccaggactgg tatgttgggt cttatagtat ttttggtggc gttaacagtt
     9361 tgagcgacta tcagacagat tttcctctgt ttgaattctc caaaatattt ggagatgaag
     9421 agtatggttt cccgctttca gttactgttt tacgctatgg ttctacctac cgtttgttct
     9481 cctttgtggt agatatgctt aatcaagaaa tgggacgaaa cttggaagtt attcaacgtc
     9541 atggggccct gctcttggtt gaaaatgggc aactcttgta tgtagaattg cctaaagaag
     9601 gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag agaaaccttg ttgattgcga
     9661 ctcgtaacga aggcaaaacc aaggaattcc gagctatctt tgataagtta ggctacgatg
     9721 tggaaaatct taatgactac cctgacctgc ctgaagtagc agaaacaggt atgacctttg
     9781 aagaaaatgc ccgccttaag gcagaaacca tttctcaatt aacgggtaag atggttttgg
     9841 cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt accaggcgtc tggtcagctc
     9901 gtttcgcagg tgtgggagca actgaccgtg aaaataatgc caaactcttg cacgaattgg
     9961 ccatggtctt tgaactcaag gaccgctcgg ctcagttcca cacaacccta gtcgtagcca
    10021 gcccaaataa ggaaagttta gttgttgaag cagactggtc aggttatatt aactttgaac
    10081 ctaagggtga aaatggcttt ggctatgatc ccctcttcct tgtaggagag acaggtgagt
    10141 catcagctga attaacccta gaagaaaaaa atagtcaatc tcaccgtgcc ttagccgtta
    10201 agaaactttt ggaggtattt ccatcatggc aaagcaaacc atcattgtaa tgagcgattc
    10261 ccatggcgat agcttgattg tggaagaagt ccgtgatcgc tatgtgggca aagtcgatgc
    10321 cgtttttcat aacggcgatt ctgaactacg tccggattct ccactttggg agggcatccg
    10381 cgttgttaaa gggaacatgg acttctacgc cggctaccca gaacgtctgg tgactgagct
    10441 tggttcgacc aagattatcc aaactcatgg tcacttgttt gacatcaatt tcaactttca
    10501 aaagttggac tactgggctc aggaggaaga ggccgctatc tgcctctatg gtcacttgca
    10561 tgtgccaagt gcttggttgg aaggcaagat cctctttcta aatccaggtt ctatcagtca
    10621 accacgaggt accatcagag aatgtctcta tgctcgtgtg gagattgatg atagttactt
    10681 caaagtggac tttttgacac gagatcacga ggtgtatcca ggtttgtcca aggagtttag
    10741 ccgatgattg ccaaggagtt tgagactttc ttgttggggc aggaggaaac ttttttgacc
    10801 cctgctaaaa atctagctgt gttgattgat acccacaatg cggatcatgc gaccctcttg
    10861 ctcagtcaga tgacctatac ccgtgttccc gttgtgacag atgaaaaaca gtttgttggg
    10921 acgattggac tcagagatat tatggcttat cagatggagc atgacttgag ccaagaaatc
    10981 atggcggata cggatatcgt tcatatgaca aaaacggacg tagcggttgt ttcgcctgat
    11041 ttcaccatta cggaggtctt gcacaagcta gtagatgagt ccttcttacc ggttgtggat
    11101 gcagagggta ttttccaagg gattattacg cgcaagtcca tcctcaaggc cgttaatgcc
    11161 ctcttgcatg actttagtaa ggaatatgag attcgatgcc aatgagagac aggatttcag
    11221 cctttttaga ggaaaagcag ggcttgtctg tcaattccaa acagtcctat aagtatgatt
    11281 tggagcaatt tttagacatg gtaggtgagc ggatttctga gaccagtctc aagatttacc
    11341 aagcccagct agccaatcta aaaatcagcg cccagaagcg aaagatttcg gcctgtaacc
    11401 aatttctata ctttctctat caaaaaggag aggtggacag cttttaccgc ttggaattag
    11461 ccaaacaagc tgaaaagaag acggaaaagc cagagattct atacctagac tctttttggc
    11521 aggaaagcga ccatccagag ggccgcttgc tagcgctctt aatcctagaa atggggctct
    11581 tgcccagtga gattttagcc atcaaggttg cggacatcaa tctggatttt caggtgttgc
    11641 gaatcagcaa ggcttcccaa cagaggattg tcaccattcc cacggccttg ctttcagaat
    11701 tggaaccctt gatggggcag acctatcttt ttgaaagagg agagaaaccc tattctcgtc
    11761 agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa aggttttcca tccttatcag
    11821 ctcaagtctt acgtgaacag tttattctaa gacaaataga aaacaaggtc gatttgtacg
    11881 aaattgcaaa aaaattagga ttaaaaacag tcctgacctt agaaaaatat agataatgga
    11941 tattaaatta aaagattttg aaggacccct ggacttgctc ttgcatctgg tttctaagta
    12001 ccagatggat atctacgatg tgcccattac ggaagtcatc gaacagtatc tagcctatgt
    12061 ctcaaccctg caggccatgc gtctggaagt gacgggtgag tacatggtca tggctagtca
    12121 gctcatgctg attaagagtc gtaaactcct tccgaaggta gcagaagtga cagacttggg
    12181 ggatgatctg gagcaggacc tcctctctca aatcgaagaa tatcgcaagt tcaagctctt
    12241 gggtgagcac ttggaagcca agcaccaaga acgggcccag tattattcca aagcgccgac
    12301 agagttgatt tacgaagatg cggagcttgt gcatgacaag acgaccattg acctcttttt
    12361 ggctttttca aatatcctag ccaagaaaaa agaggagttt gcacaaaatc acacgactat
    12421 cttgcgggat gagtataaga ttgaggacat gatgattatt gtgaaagaat ccttgattgg
    12481 acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc cagaatgtcc aagaggtcat
    12541 caccctcttt ttggcaaccc tagagttaat caaaacccag gagctgatcc tcgtgcaaga
    12601 ggagagtttt ggagatatct atctcatgga aaagaaggaa gaaagtcaag tgcctcaaag
    12661 ctagacttga tagagaggaa agatgagtac tttagcaaaa atagaagcgc tcttgtttgt
    12721 agcgggtgaa gatgggattc gggtccgcca gttagctgaa ctcctctctc tgccaccgac
    12781 aggcatccag caaagtttag gaaaattagc ccagaagtat gaaaaggacc cagattccag
    12841 tttggctttg attgagacaa gtggtgctta tagattggtg accaagcctc aatttgcaga
    12901 gattttgaag gaatactcta aggcgcctat caaccagagc ttgtctcggg ctgcccttga
    12961 gaccttgtcc attattgcct acaaacagcc gattacgcgg atagaaattg atgccatccg
    13021 tggagttaac tcgagtggag ccttggcaaa gttgcaggct tttgacctga taaaggaaga
    13081 cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg actacggatt atttcctaga
    13141 ttacatgggg ataaaccatt tagaagaatt accagtgatt gatgagcttg agattcaagc
    13201 ccaagaaagc caattatttg gtgaaaggat agaagaagat gagaatcaat aagtatattg
    13261 cccacgcagg tgtggccagt aggagaaaag cagaagagct gatcaagcaa ggtttggtga
    13321 cggttaacgg ccaagtggtg cgtgaactag caaccactat caagtcaggc gacaaggtcg
    13381 aagttgaagg tcaacctatc tacaacgaag aaaaggtcta ttatctgctt aacaaaccac
    13441 gcggtgtcat ttccagtgtg acagatgata agggtcgcaa gacggttgtc gacctcttgc
    13501 ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga ctgggataca tcaggagtct
    13561 tgattttgac caatgatggg gactttacag acgagatgat tcaccctcgt aatgagattg
    13621 acaaggttta tgtcgcgcgt gttaaaggtg tggccaataa ggacaatctt cgccccttga
    13681 cccgtggtct tgagattgat ggtaagaaaa ccaagccagc tgtttatgaa attctcaaag
    13741 tggacccagt caaaaatcgc tctgtggtgc agttgaccat ccatgaaggg cgtaaccatc
    13801 aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga taagttgtct cggactcgtt
    13861 tcggacacct agacttgaca ggactccgtc caggagaatc ccgtcgtctt aataaaaaag
    13921 aaatcagcca actacacacc atggctgtaa ctaagaaata atgaaacgaa ttttaatagc
    13981 gcctgtgcgc ttttaccaac gttttatctc accagtcttt ccaccctctt gtcgctttga
    14041 tctgacttgt tccaactaca tgattcaggc tattgaaaaa catggtttta agggagtatt
    14101 gatgggcttg gctcggattt tacgttgtca tccctggtcg aaaacaggta aggaccccgt
    14161 tccagaccac ttttccctta aacgaaatca agaaggggaa tgaggtgggg taaatagatt
    14221 tcaaaatgat aaaaacgcat cctatcaggt ttgagtgaac ttgataggat gcgttttaga
    14281 atgtcaaaat tttatactct tagaaaatct cctcaaaccg catcagcttt catctgcaac
    14341 ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctctt tgattttcat
    14401 tgagtattaa attgagtttg aagtggctta tttcaaagct ttttgtatgt cttcaatcat
    14461 gagttttgtt gattcaagtc cgcctccgct tagataccag aggtctggtg ttagttggat
    14521 aatcttacca tttttagcag caggtgtttc agcgataagg gcattttcta ggacaccgtc
    14581 gttgctagag ttgtccccac cgatggcaag ggtacggttg atgacaaaga ggatgtcagg
    14641 gttgatttct ttgacacttt caaagctgac ttcttgtccg tggcgtgagt cttcaaatgt
    14701 tgtatcagtt ggtttgaatt tcaaggtttg gtacaagaaa gagaaacgag atttggcacc
    14761 aaaggctgcc atttttcctt cattaaggag gatcgcaagg gcttttttgt cagagctttc
    14821 atttttagta gcgatttctt ggatgctctt gtctagcttg gccaattctt ccttggcttt
    14881 ctgtgtacca gtttcgccga aggcacttgc taaggattcg atattagcct tggtagaagt
    14941 ccagtagtcg tccttgcttg cttggaagag aacggttggg gcgatttctt tgaatttgtc
    15001 tacgaatttt tgtgtacgtg gtgaagcgat aatcaaatca ggctcaaggg cggcgatagc
    15061 ttctaaatca ggttctttca tagaaccaac atttttgaca gttcccacta ggtcttttag
    15121 ataagtcgga acagtttttg taggcattcc gacgatattt ttttcaaatc ctaaagcgcg
    15181 aatagtatcc gcagcgccga ggtcaaaggt cacaatcttt tcaggaactt tggaaagttt
    15241 gacctcgtcc agtgaacttt taatggttac ctctgttgga gcagagctac tggtctctgt
    15301 ctgactagtg cttgagtttg tactacatgc accaagtagg agcaagaagc tggccactag
    15361 ggcagtgaaa taaagtttaa gggatgtttt cataatttct cctttttaaa atgtgataac
    15421 gatttaggga gtctcttaat cttattgact aagagactga aggttctcta acttgagctt
    15481 ttatgttact agctatagat acagatcttt ttgtcattga tatcagctag cgtgatggga
    15541 atctcataaa gttgactgag caggtcagcc tgcatgattt gatcggttct tcccttgcta
    15601 aagacctggc cgtccttgaa ggcgacaatt tcatctgcat actgactggc catgttgata
    15661 tcgtggagga cgatgataat ggtcttgccg agttcctcca ccagtcgtcg aagaatctgc
    15721 atcatgctga cgctttgctt gatatcgaga ttgttgagtg gttcgtccag caagataaag
    15781 tccgtatcct gggccagtac catagcgata aagacgcgct ggagttgccc ccctgacagg
    15841 ctattgatgt agcggtcttt taagttggtc agttccaaat agttcagagt ttctcggatt
    15901 ttttcccagt cttctgatct aagtcgacct cggctgtagg gaaaacgtcc aaaactgacc
    15961 agttcttcaa cagtcaattt ggcttggtaa ttgattttct gttttaggat ggttagttct
    16021 tgggccagtt cttgcgaatt ccagctctcg atttcacgtc ctttgatact gagaactccc
    16081 tgatctttct tggttagtct gctcatgatg gagaggagag tcgattttcc agcaccattt
    16141 ggaccaataa aggctgtcag tttttgagga ctgacttcaa gcgaaatgcc ttgcaaaata
    16201 tcctgttttt gaatggattt gtcaatgttt tccagtttca ctgacgagac ctcctatata
    16261 gtaagataaa gaataagaag ccacccacac tctcaatgat catactgata cgaatttcca
    16321 gtgcaaagac tcgttcaatc aaggcttgcc ccaaggttaa actaataaat ccaaccagaa
    16381 tggccactat aaagagtaac ttgtgctgat agtctttgac aatcaggtag gtgaggttgg
    16441 ccagcataaa gccgaagaag gccataggtc ctaccaaggc agtggccgtt gaggtcaaaa
    16501 gcacgattcc ccagaggagc tctttctgtt ctttttcaac atcgagtccc aatatctgag
    16561 ccgtttctct ttgcaggtgc aagacatcta gaacgactgc ttttcgaaag aaaaagattg
    16621 tcaaagcgag gatgatcaga gaaccgatgg ctaggatgga agtgttaaga tgttgaaagg
    16681 aggcaaaaag actattttgc agtttatcgt attcgtttgg atccattagg acttgaagga
    16741 aggtgctgat atttcgaaag agacttctga gcgctagaca gatcagcagg atgaagacca
    16801 ggtcttgctt catcagtgtc ttcaagtaac cttgtaaggc gagaaagaag agggactgga
    16861 caagaagtaa gactaggaat tctaagatag gggatttgcc aagttgaaga aacttgcttt
    16921 caaaaaccag tagtagggtt tgtagtagga cgtagaagga ttcaattccc aaaatactag
    16981 gcgtcaggaa gcgattttcc gtcagggttt gaaaactaat ggtcgaaatc ccagtcgcga
    17041 tggctaccaa gagataaacg atgatctttt gggaacgcaa cttccaagca aaggctgaca
    17101 agtgagtgat gggccaaaag tagagaagac aagctccgat ggcaagaata atgagaatcc
    17161 agaagagctt ggtatgtttg cttttagtct gcatcttttc gtccccctct ccagagaagt
    17221 aggataaaga cgagactacc gatgattcct agcaagagac tgacagacaa ctcatagggc
    17281 ctaatcagaa ctcgggatag gatatcgcaa gccagaacta gattggcacc aaccagtgcg
    17341 accatgagtt tggtttgact tagattatct ccatagcgct tgcgaacaag attgggaacg
    17401 ataacttcga gaaatggtag gccacccacg gtaatcatgg tgacgcttgt cgttagcgcc
    17461 accagaaaga gggccagttt ttcaagtagg gagtaggaaa tccccaaact ctcgctggtt
    17521 tctttcccta gattcatgat ggtgaaggtt tgggataatt tccaaacggt tatcaggatg
    17581 atgaggccta agaagagcca ctcatactga tgggtctgaa tcatggagaa ggagccctgg
    17641 gtccaggcag tcatactctg aaccagattg aaacgatagg cgataacttc tgtgactgag
    17701 ccgataatcc cgctatagat gatcccaatc agaggcaaca tccacctttc ctttacagta
    17761 aaaatggtca taaaggctag gaagaagagg gtgaatacga tggatgaaac aaaagcgaag
    17821 agcatctttt gggtcagact agccgatgga aagacaaaaa ggctcagcac cattcccagt
    17881 ttggcggctt cagtcgttcc aactgtactc ggtgcagcaa actgattttg ggtaatagtc
    17941 tgcatgagaa ggcctgccat actcatacta gaggcagtca ggagaatact gatagttctt
    18001 gggagacggg actcttgaaa gaggagccag gtctgctggt cgaaatcaaa tagctttccc
    18061 catgaaaaat cactggtccc aatgctaata gagagaaaga ctaggagtag aagtaagcca
    18121 attaaataat gagaaagttt cataccccgt cctttcatgt agatttggta tcgaaagata
    18181 cctgcggata taaatgtaac attatttttc taatctgtca ataaaatttc tgacaattta
    18241 ataaatacaa caaggagaga gcaacaagac tttctccttt gttatcctat tctaaaatgt
    18301 ttttacctta atctgataaa ataatatctt cgagggagta gctagccgtc caatcaagat
    18361 attgtttagc ttttgaagca tctgctagga cactggctgg gtcactagca cgtcgagcaa
    18421 caatctcgtg tgggattttt taatttagta attcttcagc agttttaaag atttctttga
    18481 tagtatagcc ttttttagtt cctaagttaa agatttgaga agaactgtct tcttgaaata
    18541 ggtagttcat tcctttaaca tgagcctatg caaggtccaa gacataaatg taatctcgaa
    18601 tacatgaacc gtcacgtgta tcgtagtcat ctccaaatat ttttaagcta tcattttgtc
    18661 ccaatgcggt cttgttgata tttggaatga tgtgagttgg atttttcaca cgcagaccgt
    18721 ttgaagcatc catttcagcc ccagcaacat taaagtaacg gaaaataaca tatttccagt
    18781 cgtagcgatt ggccatccag taaatcattc gttcgcccat cagttttgtc tctgcataag
    18841 ggttgacagg gtcgagcagg gtatcttcag tcgccggctt gtcaatacag ttatttccat
    18901 agagagaagc agtcgaagag aacatgattt tttgaatgcc aacttcagat aagactttga
    18961 gaacttggtt cataccagca acgttggcag tgaagtattt acttggattt tcaatacttt
    19021 cgctcacaac aatttcacct gcataatgga ggacagcatc aatctgattt tcttctaagt
    19081 aagcctttaa ggcgctagca tcataaacat ccagttcttt aaagctagca cgactatcca
    19141 cagccgctcg atttcctgtg gagagattat cgaggacatg caccttataa ccagcattta
    19201 aaagagcttt aacggtatgg gagccaatgt agctagtccc acctgtaaca agaattgttt
    19261 tggtcatgat tttttccttt tctagttttg tactatagta aattgaaata agatatgaac
    19321 aactctatta ggaaagtcaa attaatttct
//
LOCUS       DAVMHG010000028        19180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMHG010000028 DAVMHG010000000
VERSION     DAVMHG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.028"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1403
                     /locus_tag="VYR08_000772"
     CDS             <1..1403
                     /locus_tag="VYR08_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466338.1"
                     /translation="RSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGIRIINTD
                     DSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQINKVIK
                     RQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQLLELVYKE
                     TSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEGVAQSF
                     ESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQISV
                     KYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDIKKE
                     LSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFV
                     AETLSYYCSNNFELEVWTELELSKESLEDSSYDIIISNFIIPPIENKRLIYSNNINTV
                     SLIYLLNAMMFIRLDE"
     gene            1765..2760
                     /locus_tag="VYR08_000773"
     CDS             1765..2760
                     /locus_tag="VYR08_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3466339.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIAIPTYFWGDKTTKKI"
     gene            2887..4197
                     /locus_tag="VYR08_000774"
     CDS             2887..4197
                     /locus_tag="VYR08_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV3466340.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4293..5195
                     /locus_tag="VYR08_000775"
     CDS             4293..5195
                     /locus_tag="VYR08_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3466341.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYTFTDYYLLTPDERKFVGFENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5197..6042
                     /locus_tag="VYR08_000776"
     CDS             5197..6042
                     /locus_tag="VYR08_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3466342.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFLGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRFAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6052..7371
                     /locus_tag="VYR08_000777"
     CDS             6052..7371
                     /locus_tag="VYR08_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3466343.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVHQVQNLAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHMDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7579..7651)
                     /locus_tag="VYR08_000778"
     tRNA            complement(7579..7651)
                     /locus_tag="VYR08_000778"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7616..7618),aa:Lys,seq:ctt)
     gene            complement(7916..9187)
                     /locus_tag="VYR08_000779"
     CDS             complement(7916..9187)
                     /locus_tag="VYR08_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV3466344.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            9457..9696
                     /locus_tag="VYR08_000780"
     CDS             9457..9696
                     /locus_tag="VYR08_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466345.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            9861..10040
                     /locus_tag="VYR08_000781"
     CDS             9861..10040
                     /locus_tag="VYR08_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466346.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10177..10355
                     /locus_tag="VYR08_000782"
                     /pseudo
     CDS             10177..10355
                     /locus_tag="VYR08_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            10392..10844
                     /locus_tag="VYR08_000783"
     CDS             10392..10844
                     /locus_tag="VYR08_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV3466347.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11135..11605
                     /locus_tag="VYR08_000784"
     CDS             11135..11605
                     /locus_tag="VYR08_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV3466348.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11624..12712
                     /locus_tag="VYR08_000785"
     CDS             11624..12712
                     /locus_tag="VYR08_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV3466349.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFIFHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENSKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            12693..13121
                     /locus_tag="VYR08_000786"
     CDS             12693..13121
                     /locus_tag="VYR08_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3466350.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECAAREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSLFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            13118..13591
                     /locus_tag="VYR08_000787"
     CDS             13118..13591
                     /locus_tag="VYR08_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466351.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            13726..14679
                     /locus_tag="VYR08_000788"
     CDS             13726..14679
                     /locus_tag="VYR08_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV3466352.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNT
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            14681..15424
                     /locus_tag="VYR08_000789"
     CDS             14681..15424
                     /locus_tag="VYR08_000789"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV3466353.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDG
                     IKRLARVLDVEARQFELVEELDDNVELPVQVTISSGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15435..17231
                     /gene="pepF"
                     /locus_tag="VYR08_000790"
     CDS             15435..17231
                     /gene="pepF"
                     /locus_tag="VYR08_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3466354.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALINLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            17478..18269
                     /locus_tag="VYR08_000791"
     CDS             17478..18269
                     /locus_tag="VYR08_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV3466355.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIVEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            18380..19048
                     /locus_tag="VYR08_000792"
     CDS             18380..19048
                     /locus_tag="VYR08_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV3466356.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         5893 a         3212 c         4205 g         5870 t
ORIGIN      
        1 atcgttctga actagcagag ttattaaatt gtacagaacg tgcagtcaaa gatgatctat
       61 cccatgttaa atctgctttt cctgacttga tttttcattc ttctactaat ggtatacgca
      121 ttattaatac cgatgatagt gatattgaaa tggtttacca tcatttcttt aaacattcaa
      181 ctcatttttc gattttagaa ttcatcttct ttaatgaagg ttgtcaagct gagagtattt
      241 gtaaagaatt ttatatcagt tcatcttcgc tctatcgtat tattagccaa atcaataaag
      301 tgattaaaag gcaatttcaa tttgaagtca gtctgacccc tgttcaaatc attggaaatg
      361 agagagacat tcgttacttt tttgcacaat atttttcaga aaaatattat ttcctagaat
      421 ggccatttga aaatttttca tcagagccac tatctcaatt gttagaattg gtttataagg
      481 aaacaagctt tccaatgaat ttgtcaacgc atagaatgct aaagttgctc ctagttacga
      541 acctatatag aataaagttt ggtcatttta tggaagtaga taaagattct tttaacgacc
      601 aaagtttgga ttttttaatg caggcagaag gaatagaagg tgttgctcag agttttgaat
      661 cagaatacaa tatctcttta gatgaagaag ttgtttgcca attatttgtg tcttactttc
      721 aaaaaatgtt tttcatagat gaaagtctct ttatgaaatg cgtaaaaaag gatagttatg
      781 ttgaaaaatc ttaccatcta ttgagtgatt ttattgatca gatttcagtc aagtatcaga
      841 ttgaaatgga gaataaggat aatctgattt ggcatctgca taataccgca catctgtatc
      901 gtcaagagtt gtttactgag tttattttat ttgatcaaaa agggaataca atcaggaact
      961 ttcaaaatat ttttccaaaa tttgtttcag atataaaaaa agagctttct cactatttag
     1021 agactcttga ggtttgttct agttcaatga tggtaaatca tctatcttat accttcatca
     1081 ctcataccaa acatttggtg attaatttgc tacaaaatca gcctaaacta aaggttttag
     1141 ttatgagtaa ttttgatcag tatcatgcaa aattcgttgc agagacactt tcttattact
     1201 gtagcaataa ttttgaactg gaagtttgga ctgaattaga attatcaaag gaatctttag
     1261 aagattcatc ttatgatatc attatttcta attttattat tcctcctatt gaaaataaga
     1321 gactcatcta ttcaaataat ataaacacgg tctcactcat atatttgtta aatgccatga
     1381 tgtttattcg attagatgag taaccaaaaa taacaaaaaa acgctatttc gcgtttgaac
     1441 tgtacctcaa aagttagaca gaaaaagtct aatttttggg gtgtttttgt taagaaaata
     1501 ccttcaatca tttaagcaaa atgtacatct agatttatag ccatctaaaa ctcttgcttt
     1561 ttttgtagaa gatacgaaaa aagccatgaa accaaggctt ttcctgtttt atgtagatgt
     1621 ttacacatga aaattgaagt attttcaaaa gctaattgga gtgattttat tcatgatggg
     1681 ataaatgatc tggccaaaaa tgattgagaa atagttgctt aaaaagcatt tgcatgatac
     1741 tatatattga aaggaggtga taatatggta gaacaaagaa aatcaattac catgaaagat
     1801 gttgctttag aagcaggagt tagtgttgga actgtttcac gtgtaattaa taaagaaaaa
     1861 ggcattaaag aagtaacttt aaggaaagtt caaagggcta taaaaacatt ggactatatc
     1921 cctgataatt atgctcgagg tatgaaaaag aatagaacag aaactatcgc tttgattatt
     1981 ccgagcatat ggcatccatt tttttcggag tttgcctatc atgttgagaa agagttatta
     2041 aaatataatt ataaattatt tatttgtaac gctgatagcg attcacaaaa tgaaatcgaa
     2101 tatatcgaaa tgttgaaaca aaataaagtg gatggaatta ttggtattac atattctgat
     2161 attgacaaat atattttatc tgatttaccg tttgtaagta tagatagaca tttttcggaa
     2221 aatgtatgct atgttacttc agcaaattac aaaggaggac aaactgcagc agaggaactg
     2281 gttagaaggg ataataagca ccttgctttt attggttcaa ttaatatgta tgagaatgag
     2341 actatactca gaagtaaagg attcagagaa aaagctaagg aattaggagt agatgtctat
     2401 aattttgaaa tgaaagaacc tttaataaat cctgaagatc agataagaat ttttttgcaa
     2461 tctcataaag aaattgatgg tatatttaca ataaatgata tgatggctct gagagttatt
     2521 agtgttatga aagaactggg gaaaatagca cctgatgatt accagataat tgggtttgat
     2581 ggtttaaaaa cttctgtcga tcaatcttat ctagtgtcta caatagttca agatattggt
     2641 atgatggcaa aagaatctgt tgcaatgata ctgaaaatga ttaagcgaga agagattcta
     2701 gaaaaacgaa tcgcaattcc cacatatttt tggggagata aaacaacaaa aaaaatttaa
     2761 aaagcgcttg acaattaatg aaagcgctgt tataatataa tcaacaaatg aaacgtttca
     2821 aaaataggct agaagcttgt ttaaaaaata aataaaaaat gaaacgtttc aaaaaggagg
     2881 aaaatgatgc gtaaaggtac aatgttatct gttgttgcgg ggctatcgtt agctatactt
     2941 gcaggttgta gtggaggaac taactcaaaa caagcgagtt cttcaaatga tattaaagtc
     3001 tgggtacaat tctctgacga aacaaatgaa ggaaaagctt ggcaaaaagt tgttgataac
     3061 tttaatcaat caggaaaaag taagtataag gttgtaactg agtatattcc tcgtagtggt
     3121 agtggtggag gatatgaaga caaggttaat gctgctatta cgactaatag tcttccagat
     3181 gttattaccc ttgatggacc aaatacggca gcttatgcaa aatctaaagt aattactccg
     3241 ctagatgatt atctaaaaga taataatatg gatgatgttt tggacagtat taaacaacag
     3301 ggaacttata atgggaaatt ctatgcattt ggattttctg aatcgaatgt tggtgtttat
     3361 tacaataaaa aaatgttcaa agaggccgga attgcggaat cagagcttcc aacgctagaa
     3421 aagccatgga catgggacga atttaatacg attgctaaaa aattgaaaga tcattataac
     3481 aaaccagcaa ttgattttcg tattaattca aacgatgaaa tgttgccata tgcttatatg
     3541 ccgttgattt ggtcaaataa tggttctgtc gtaaacgaag atggaacaaa ggcagaagga
     3601 tacttcaact ccaaagagag tgtagaagca gttcaattca ttcaaaatct tgtaaaagaa
     3661 ggctatacaa ctgttagtcc agtcgaaaaa ggttttgaaa caggagagta tccaatgctt
     3721 ttgagtggtt cttggacgat tgctgacatg aatacaaact acaaagatat tgattttggt
     3781 atccttcctt acccagtatc tagtaagacg aaaaaattag tatccccttc aggtagttgg
     3841 caattagctg tgacgacaaa atctgataaa aaagaggccg catctgaatt tgttaaattt
     3901 gctacaaata cagaatcaag tgaaattatt agcttgggga attcggtcct tccaattcgt
     3961 aaatctacaa ttgaaaatat caaagataaa gtatccgaac caatgcgttt cttgatggaa
     4021 caaaattcaa aaacagctca tgctcgccca gttgtcgtag catatcctca ggtttctcga
     4081 gcttttcaac aagcaatgca agatattagc tattatgaag aaaatccaaa tgtgcaaaaa
     4141 gtcttggata ctcgtacaaa agaaatgcaa actgctattg atcagtcact caaataatct
     4201 gtaaaaaaga aggtggaagg aaaatgtttg gaactacctt cccctttctt gttaatagcc
     4261 tagtttgata tatatgatta aaggagatgg atatgagtaa agaaactgtt gtggatacaa
     4321 aaagacgtga aaaaataaaa gataacatac taggttatag ttttttagct ccagctttaa
     4381 ttttattagg tatctttttg gtaattccag ttggaatggt catttattac acttttacag
     4441 attattattt gttgacacca gatgaaagaa aatttgttgg atttgagaat tttattcgat
     4501 taacaaaaga tccgattttc ttaaagagct tcttgaatac tttgaaattt gttgtttgga
     4561 ttattccagt tcaattggga gcggccttag gaatggcttt gattgtcaat aaaaagcgaa
     4621 aaggaaatat gttttttaaa gtggcttttt ttgctccagt tgtcatgtcc ttggtagtta
     4681 tttctattct ttggctttat cttttaaatc caaatagtgg cttattaaat gctattttaa
     4741 ataaggtagg tattgcttct caacctttct tgactagtcc taaacaagct atgtatgcaa
     4801 ttgtttttgt ttctgcttgg cagggtgcag gttatcagat gcttttattt ttaggaggaa
     4861 tgcagaatat tccacaagat gtctatgaag ctgctgaatt agatggattc tcaaaatggg
     4921 ctcagtttcg ttatatcacg atgcccctac taaaaccaac agccctgttt gtattgttaa
     4981 caactttaat ttctgctttt aagctgattg tacaaccaat ggttatgacg caaggtgggc
     5041 cattaaactc aacaattact atggtgtact atatttatca acaaggtttt actgatcgtc
     5101 ttgtaggata ttctagctca atcgctttag tatttacaac gttaattggt atgattagct
     5161 tagtacaacg tcgcgttctg aaggaggatg actaagatga aacgatttaa accaacaaca
     5221 atcttagaat atattctgtt aactttattg gcaggactct tcctttttcc tatggtttgg
     5281 atggtagttt cagctatgaa gccagaagcg gatgtttata aaaatttgac tagttggcaa
     5341 gccttcttac caagtttaaa tcccgctgat tggtttaaac cttatcagga agtatttgag
     5401 cgctttagta tttttacata tctagggaac agcatttttt atgctggaac gtttgctatt
     5461 ggttctatta ttgtcaatgc actggccggt tttgcttttg caaaagtcaa ttttttaggt
     5521 aagaaaattt tatttggttt cttacttgct ttacttatta ttccagtgga aactgtgtta
     5581 attccacaat ttaccattat caattcctta ggtttggtca ataatcgttt tgcagttatt
     5641 attccaggat tggcgagtgt atttaatatt tacttattta ggaatttctt tattgcaatt
     5701 ccagaagaga ttcttgagtc tgcaaagatg gacggagcaa gtatcgttag aattttcttt
     5761 agaattatgc ttccaatgtc taaacctgca gttgctacag ttggtgtttt atcatttatt
     5821 tcaagctgga atgattatat ctggccattg atggttttga cagattcttc taaattttca
     5881 atgcaggttg caattacaac tattaataca actcaaccag tttatatcaa tcaggtaatg
     5941 gctgtattaa ctatttcaac gattccgttg attttggtct atgtagtcgc tcagaaatat
     6001 attgttcaag gtctgggtgg ttccggaact ggaataaaat aggatatact tatgataaat
     6061 aaaacattta cagtagaaaa agccaatcgt tttatagcag aaaataaaca tctcgttaat
     6121 actcaatata agcctgaaga acatttttca gctgagattg gttggatcaa tgatccaaat
     6181 ggatttgttt attttcgtgg agaataccat ctcttttatc aattctatcc atatgatagt
     6241 gtttggggac ctatgcactg gggacatgct aaaagtaagg acttggtgac ttgggagcac
     6301 ttgccagtgg cacttgctcc tgaccaagat tatgaccgaa atggttgttt ctcaggctct
     6361 gccattgtca aggatgatcg cctctggctc atgtacactg ggcatatcga agaaaaaacc
     6421 ggtgtccacc aagtgcaaaa tctggcattt tcagatgacg ggattcactt tgaaaagatt
     6481 tcccaaaatc cagttgtaac tggaacagac ttgccagatg agttgattgc tgctgatttc
     6541 cgtgatccaa aactctttga aaaagatgga cgctattact ccgtagtagc tgccaaacac
     6601 atggataatg tgggctgtat cgttctacta gggtccgata acctagtaga atggcagttc
     6661 gaatccatct ttttaaaagg gggagaacac caaggcttta tgtgggaatg cccagattac
     6721 ttcgagttag atgggaaaga ttgccttatt atgtcaccca tgcgttatca gcgtgaggga
     6781 gactcatatc ataacatcaa ctcatcgctt ttgttcacgg gtaaggtaga ttggagagaa
     6841 aaacgtttta tcccagaatc agttcaagaa attgatcatg gccaagactt ctatgcgcct
     6901 caaacattgt tggacgatca aaatcgtcgt atcctgattg cttggatgca gacatggggg
     6961 cgtacccttc caacccatga ccaagaacac aaatgggcat gtgccatgac tttacctaga
     7021 attctaagat tggaagatgg caaactaaga caattccctg ttaaaaaagg ccaatatcaa
     7081 atccaaatag ataaagattg tcattaccac ttaggaaatg atacagatta tcttgaattt
     7141 ggttatgaca gtaatgcgca gcaagtttac attgatcgta gccatcttat tcaaaaaatt
     7201 ctaggtgaag aagaacagga cactagtcga cggtatgtag atattgaagc taaagaattg
     7261 gaagttgttc tagataaaaa ttccatcgag atttttgtca atcaaggtga agcaagcttg
     7321 actgcaactt attacttaac ggtgccagct gagctatcac gaattgatta aaaattaagt
     7381 tatttctcct aaagaaaaag ttctctttct aaaatagtgg aaagaggact ttttgtgttt
     7441 tgggtatata agcttagttt atggtatttg taaaattggt gttggattat gatttaagct
     7501 agttttctaa agaatttgaa aaaaatttta tttaagcaaa aaaaccttgg ttccaaggct
     7561 tttcctgttg tatttatatg ccccctacag ggatcgaacc tgtgacccac ggattaagag
     7621 tccgctgctc tgccagctga gctaaggagg caaagaaaaa agctgtattg gtgccgaaac
     7681 ttcacggttt gtattgaacc cgcgcaatta agcaggtggg caactcgctc taactgaagc
     7741 tgtttccgtg tgagacggcc tacatgctgt tagaagactt ttgtttccct aataatacaa
     7801 aaaatagtcg gtcaacactt aagtgtgaag tcgtacacca cagcgtttct atatttatat
     7861 gataccactt ttttaaaaaa aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga
     7921 tttttcgcaa atggtcgata gcttcctttc tttgagccaa agcccgttca tatttaccag
     7981 tatcttctgc ttggaaatag tgatgattac gaattttttc tggcagatag tcttgcttta
     8041 cccaatttcc aggatagttg tgtggataga gatagtcttg ggcattcccc agttccttgc
     8101 ttccactgta gtgcccatca cgtaggtgtc gcggaatagg caagtgccct gatgttttga
     8161 ggtcagcaag tgccttatcc atagctacat aggctgagtt ggattttgga gaaagggcca
     8221 aatcaatcac gacattggca atgagaatgc gggcttctgg gaacccaatc ttctgggcag
     8281 catccagagc agtcacggta tgaatctggg cctcaggatt ggctaagccg atatcttcat
     8341 aggcgataac agtcaagcga cgagcgagac taggcagatc cccagcctca atcaagcggg
     8401 cagcatagtg gagactggca tccacatctg agccacggat agacttttgc agggcagata
     8461 gaacatcata gtgaccgtct ccatccttgt ccatagtgat gtagcttctc tgaagactat
     8521 tttccatgat gtctaaggtg atgtggcgaa tgccctcgtc attctcaggg gtagagagaa
     8581 cagccaagtc cagtgagtta aaggcagagc gaaggtctcc gtttgtagag gttgcgatga
     8641 aatccagcgc atcctcatct agttctattg gaaaatcaaa accacgttca gggttactta
     8701 gagctatctg cagggcctct ttgacgtctt ggttagacag aggttccaac tcgaaaattt
     8761 gaacacggct acgaatggca ggagtgacag agaagaaagg attttcagtc gttgctccaa
     8821 tcatgatgac cagtccactt tccaagagag gcaagaggaa gtcttgcttg gtcttatcta
     8881 gtcgatgaat ttcgtctagc aataggacga gaccaccaga aaatttagct tcttccgaga
     8941 tttcttgcag tcgcttttta ctatcaactg tcgcattgaa agttcgaaag gcatacttgg
     9001 tcgttccagc gatggcagag gcaatactgg ttttgccgat tcccggaggg ccatatagaa
     9061 tcatggagga caggcggttg gcttccacca tgcggcggat gatttttcca ggtccgacca
     9121 gatgctcctg accgatgacc tggtcgatgg ttttagggcg catgcgaagc gcgagattgt
     9181 ctggcatagc agtcctttct aacgtggatt tgagtagaat gactcatcta ctatatctta
     9241 ctatgtccat tctagcctat tagttgtgat atttcaatgg ttttgtaata atcagggagt
     9301 gagatagaat cttttttact atttaattta atgaggagag gtatcagcat gagagatttg
     9361 attttcctta aagctcaaaa taggatagtt gagcctaaag aagtggaagt gctgacttga
     9421 gaaataatat tgctgaatgt cattgtatga actataatga aaagagaggt gataagcaat
     9481 ggaaatgatg gaccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt
     9541 cttagtgatc aagttatttc agggaaaaga aaacttttct tccgggaaga tactccaaaa
     9601 gagattttag atttgtacga aaacattaaa tctaaacttg attttgctta ccaagaagtt
     9661 cattctaata atgggttgaa aaaatatgaa aaatagaata attgatgttt ttgaagtagt
     9721 aaatcgcctc cttgtaataa ctgttgaaaa tcctgatttc gaagacttga gagttaatca
     9781 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg
     9841 taaggcgacg ttgacgtggt ttgaatttga ttttcgaaga gtatcagttt gtaaaaattg
     9901 gagataaaaa atatagagta cgcagtgttc ctatgattca ttcaactccg cctcaatctg
     9961 tcttagatcg agatactttt acaattgact atacagatga tgaattgctc gataaagaag
    10021 cagtttttac tactcattag aaaaataggt ctgaataaat tggcttccct tagaaccaca
    10081 tctaggggct tttatgtata atagaataat aagtatcact cctttacaat acgtgtaaca
    10141 cgtgttataa tggtgttgtc aggaggtaaa agcgctatgc ctatgacaca aaaagagatg
    10201 gttaaactcc ttacggccca tggttggata aagactagag gcggtaaagg ctcccatatt
    10261 aaaatggaga agaaggggaa agacttatca caatccctca tggtgagctg aataagtaca
    10321 ctgaaagagg gataagaaag caagctgggt tgtaaaattc agcccctgtt ttttgatatg
    10381 gaggtagtga aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa
    10441 cagaagcgac ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg
    10501 ggatttctga ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga
    10561 tagagagtga tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata
    10621 atgatccttt taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta
    10681 tttctatggt atctgttgat gtatcagaat acctaggaag tcaggaaccc ataaaaaaga
    10741 ctttgaccat accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc
    10801 agactttgac agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc
    10861 gtggtaatag tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta
    10921 tagatttatt agccctttcg cgggcttttt atattgattt ttatgagaaa tgaagaaatc
    10981 agacttttaa gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg
    11041 acataaaata attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg
    11101 gggcgattaa agagtcgcat agaaagagga caaaatggca acatacggat ttttagatgt
    11161 tttagaggaa gagttggaca agaactttcc ctttgacttt gagattagct gggacaaacg
    11221 caaccatgcg gtagaagtga gttttctatt ggaagcgcaa aatgctgcag gtgtggagat
    11281 ggtggatgaa gacggagagg ttttatcaga tgacattctt tttgaggaag cggtcctttt
    11341 ctacaatcct gccaagtcaa cagtcaatga ggaagactat ttgactgtta ttccttacct
    11401 gcctaaaaaa ggattttccc gtgaattttt agcttatttt gcgcttttcc ttaaagatac
    11461 tgctgaggtt gggctagatg tcctcatgga ctttttggaa gacccagaag cagaggaatt
    11521 tgtcatggaa tggaaccaag aagtctttga agaaggaaaa gttggcttgg aagagggaga
    11581 attttatcct tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac
    11641 ttatttggtt tatgtgctag cttttgtctt tattatgcta gcttttgctt gtggaacaat
    11701 cgcatttgca gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc
    11761 tcttctaagc atgtatttaa tctttatttt tcatgagctg ggtcatgctt tttgtggcta
    11821 cttgacaggc tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc
    11881 aggcaggttt catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg
    11941 tttaaaagag gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt
    12001 tcatctcggc ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt
    12061 tgcagggact tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga
    12121 catcaccgat ggagcaaaaa tttgggaatt actacaacac cctgaaaata gcaaatacgc
    12181 ctacctggta ttgagacatt ctgctcaaac tttgctggct cctcaagaat atgatttgaa
    12241 agactttgtt caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg
    12301 tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca
    12361 agcattactt gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctact
    12421 tcatatcgcc ttgttggaag gagattttga gcaagcggag caatatgcta gtattcgaca
    12481 ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cttggtatca
    12541 atttaaggta aggaaagatg tggttcaaac tcacaaggct atgaagattg ctagacagaa
    12601 aatggatagt agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga
    12661 gctggaaaag gaaatgttag aaggaactga agatggaact agaaatttct gatttcccag
    12721 gctgtaagat tgctttgttt tgtggggata agcttttgac tatcttacgc gatgataagg
    12781 caagcattcc ctgggccaat atgtgggaac tgccaggtgg gggacgtgaa ggtgatgaaa
    12841 gtccttttga gtgcgcggcg cgtgaagttt atgaagaact gggcattcat ttagatgaag
    12901 actgtctgct ttggagcaag atttatccta gcgtaatttt taaaggcaag aaatcgctct
    12961 ttatggttgg tcagctaaga caagaacaat ttgacaatat catcttcgga gatgaaggac
    13021 agggctataa attgatgcct attgaggaat ttctcaattc taaacaggca gtgcctcagt
    13081 tgcagggaag attgagagat tatttggagg aaagtttatg ataacacttg ttcgagcggg
    13141 agcagaggat ttagaaacta ttattgcaat tcagagagcc agctttaagg ctgtttatga
    13201 aaaataccat gatgaatatg acccttatct cgaggactgc gaacgaatta agtggaaatt
    13261 agtcgagcgt cccaatagct attactactt tgtaaaagaa gatgaagaaa acatcggatt
    13321 tttacgagtt cagaccaatg ccgagttaac gaaagcttgg ttgggaacgg cagcgatttt
    13381 gcctcagtat caaggaaaag ggtatggatc taaaggttta agcttactgg aaaaagaatt
    13441 tccaaccatc acacaatggg atttgtgtac ggtgttacag gatgcaggca tggttgcttt
    13501 ttacgagaaa aacggctatc gtcaaatcca cattgaggct gaaaaagaag gcatggatat
    13561 ggtctacatg aaaaaattga tagagaaata gaaaggatgg ttcagttaaa tttctaaact
    13621 gaacccgccc taaacactgt gccaaaaaga taaacttctc ttagacacaa gcgtcttcag
    13681 agaatttcct attttggttt tgtgttttac gggcttggta tcttaatgat ggaaacatgg
    13741 caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt
    13801 gagctgggag cgcaaggtgt tgcgattgaa gacagtctgg actatgtggg aaatgttgac
    13861 cgcttcggcg agattttccc agaggtcgag cagcaagaag aaatcgtagt gacagcctac
    13921 taccctgaca cggtggatgt gacagtggtt gaggcggact tgcaggctcg catatcagaa
    13981 ttgacggatt ttatggattt gggagagtta aaaataggca cgactgcctt ggctgaggaa
    14041 gactgggctg acaactggaa gaagtattat gaaccggcac gcatcaccca tgacctgacc
    14101 atcgtaccgt catggacgga ctatgaggca acagcaggag aaaagattat caagctggat
    14161 cctggtatgg cctttgggac agggacccac ccaacaacca agatgagcct ctttgccttg
    14221 gaacaggttc ttcgtggtgg cgaaacagtg ctagatgtag ggactggttc aggggttctt
    14281 tctattgcaa gctcactcct aggtgccaag gagattttcg cctatgactt ggatgatgtg
    14341 gcggttcgtg tcgctcagga aaatattgag ctcaacactg gtatggaaaa tatccatgta
    14401 gcagcaggag acttgcttaa gggagttgag attgaagcag atgtcatcgt ggctaatatc
    14461 ttggcggata ttctcattca tctgacggag gatgcttatc gcttggtaaa ggatgaaggc
    14521 tacctcatca tgagtggcat tatcaaggac aagtgggaca tggtgcgcca gtcggctgag
    14581 tcagctggat ttttcctcga aactcacatg gttcaagggg aatggaatac ctgtgtcttt
    14641 aagaaaacca aggatatctc tggtgtgatt ggaggctagg atgcaacagt attttgtcaa
    14701 ggggagtgca gtctctcctg tcaccatcga ggacagggaa accagcaagc atatgtttca
    14761 ggttatgcgc ttgaaagaag atgatgaggt tactttggtc tttgatgatg gaattaagcg
    14821 cttggcgcgt gtgctggatg tggaagctcg tcagtttgaa ttggtagaag aactagatga
    14881 caatgtggaa ctgcccgttc aagtgaccat ttcatctggt tttcccaagg gagataagtt
    14941 ggagttcatc gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc
    15001 agactggtca gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga
    15061 aaaaattgcc cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact
    15121 tttcgagaaa aaagcagatt ttctagctca gctggaccag tttgactcta tcatagtagc
    15181 ctatgaagaa tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct
    15241 tgaaacagga gccaaactgc tctttatctt tggtccagaa ggtggtctct cacctgcaga
    15301 aatcgaaagt tttgaagcta agggagccgt tttggtaggc ctaggtcctc gtattttgcg
    15361 agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag aattagagaa
    15421 ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactctggg
    15481 acttaacagc cctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgcg
    15541 aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt gaggatttcg
    15601 agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg
    15661 gttttatgcc tcagacgacg gactatagca atgacgaatt tgcaaatatt gctcaagctg
    15721 ggatggaatt tgaaacagat gccagcgtag ccttgacctt ttttgacgat gccttggtgg
    15781 cagcagatga ggaagtcttg gaccgtttgg gtaaattgcc acatttaaca gctgccattc
    15841 gtcaggctaa aatcaaaaaa gcccactact taggggcaga tgtggagaag gccttgataa
    15901 atctcggtga agttttctac agtccgcagg acatttatac taagatgcga gctggggatt
    15961 ttgaaatggc tgactttgaa gcccatggca agacctacaa aaacagcttt gtcacctatg
    16021 agaatttcta ccaaaaccat gaggatgctg aggttcgtga gaaatccttc cgttccttct
    16081 cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt
    16141 ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgactat cttctagctg
    16201 aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg aaggactttg
    16261 caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga
    16321 cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg actattgacg
    16381 atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag
    16441 ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc aaggattccg
    16501 gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc tggacaggcc
    16561 gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt
    16621 cagacaatca tcaaagttac ttcaatgccc atatgtcgac ctactatgtt gaagcaccgt
    16681 caaccttcaa tgaattgcta ctcagtgatt acttagagaa ccagtctaat gacccacgtc
    16741 aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataat ttcatcaccc
    16801 acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggagagacct
    16861 ttggagcaag caagctcaac agcattatga aggaagtttt gacagatttt tggggcgatg
    16921 ccattgaaat cgacgacgat gcagctttga cttggatgcg ccaagctcac tactatatgg
    16981 gcttgtatag ttacacttac tcagcaggac tagttatctc gactgctggt taccttcatc
    17041 taaaacattc tgaaactgga gctgaagact ggctcaatct ccttaaatca ggtggtagca
    17101 agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc
    17161 gtgataccat ccaattcttg tctgacacag ttgaccagat tatcgcctat agtgctgagt
    17221 tgggggagta aggggaatag aaaaattttc tagaatgatg tttatgggct tgttaagcgt
    17281 cattctattt gtcttagttg tagctatttt caaaaatgtc gttgaaggtc gtgatgttaa
    17341 atttgaactt actgtagaac tggtattgat agttatttac gtcattttat atcagattgt
    17401 atttaattga gaattcaata ttgagtcatt taagactgcc tttttgaaca gttgctagaa
    17461 atgtagaggt attgaccatg tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa
    17521 attataacca ggaggaaatc cagtggttgc ttgatcattt aggagatcct tctccagaaa
    17581 ttcgcgatga ccttgttttt acaagctttg ctagagggat tcaggaagag ctatttacac
    17641 aggaacaatt tcatttcatt gttgaagggg tttcatctga tgtaggacta gataaagaga
    17701 ttgataaggt aggcctgcca acactggaac gttcttttag ggcacttgtt tatgcaaatc
    17761 tcttgtctgc agatgctaac cagcaatcgg ttttttatca gggattacaa tcagaaattc
    17821 gtaatgtcct tttaaatcaa ggcttgcact atctttcaaa agaaaaggat acaacaggtt
    17881 tctcaagtca gtatggttgg gttcatgctt ttgcacatgg agccgattta ctgacagagg
    17941 tggtttgtca tccagacttt cctaaaaaca gagttcatga agtatttgat atacttggcc
    18001 aactatttaa aagaatgtcg attcgcttta cagatgatga ggattggcgt ttagcaagag
    18061 tgatctatga acctatttta caagggaagt tggagcaaga acaagtagct tcttggataa
    18121 aaactgttga ttttccgata gaagaaaggg aggattttta caaattttcc aactttagat
    18181 cctgtctggt ggaagtctat gtccaacttg accagagaaa tagtttacaa gatgacctga
    18241 aagaagctat ccagtctttt caatactagg gattagctaa tagttctatg attttcaaaa
    18301 aatttccaat caattttctt gaaacccttt cctacttttg atagactagt atctagtttt
    18361 tgaaaaaagg agaaagaaaa tgaaaaaatt tgttgctgag ttaatcggta cgttcatgct
    18421 tgtgttcgtt gggacaggag ctgttgtttt tggaaatggt cttgatggcc ttggtcacct
    18481 tggaatcgcc tttgcctttg gtttggcaat cgtggtggcg gcctactcaa tcggaactgt
    18541 ttcaggtgct cacttgaacc cggctgtttc gattgctatg tttgtaaaca aacgtttgtc
    18601 atcttcagaa cttgtaaact acatccttgg tcaggttgtt ggagctttca tcgcttctgg
    18661 cgcagtcttc ttcctcttgg gtaattcagg tatgtcaact gctagtcttg gtgaaaatgc
    18721 cttggcaaac ggtgtcactg tctttggtgg tttcttgttt gaagtcatcg caactttctt
    18781 gtttgtcttg gttatcatga ctgtgacttc agaaagtaag ggcaatggcg cgattgctgg
    18841 tttggtaatc ggtttgtcat tgatggcgat gattcttgtc ggattgaaga ttactggact
    18901 ttcagtaaac ccagctcgta gcttggcacc agctgtcttg gtaggcggcg cagcccttca
    18961 acaagtttgg attttcatcc ttgcaccaat cgctggtgga gttcttgcag cccttgttgc
    19021 aaaaaatttc cttggaacag aagaataatt gaaactcaaa aagccttgct cctcatcttg
    19081 aggaacaggg ctttttcgta tgatactctt cgaaaatctc ttcaaaccac gtcagcttca
    19141 ccttgccgta ggtatggtta ctgacttcgt cagtttcatc
//
LOCUS       DAVMHG010000029        18473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMHG010000029 DAVMHG010000000
VERSION     DAVMHG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.029"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(17..823)
                     /gene="yidA"
                     /locus_tag="VYR08_000793"
     CDS             complement(17..823)
                     /gene="yidA"
                     /locus_tag="VYR08_000793"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV3466357.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMVGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(836..2140)
                     /locus_tag="VYR08_000794"
     CDS             complement(836..2140)
                     /locus_tag="VYR08_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV3466358.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2212..2589
                     /locus_tag="VYR08_000795"
     CDS             2212..2589
                     /locus_tag="VYR08_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV3466359.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDRQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2679..3011
                     /locus_tag="VYR08_000796"
     CDS             2679..3011
                     /locus_tag="VYR08_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV3466360.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3023..4594
                     /gene="ffh"
                     /locus_tag="VYR08_000797"
     CDS             3023..4594
                     /gene="ffh"
                     /locus_tag="VYR08_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV3466361.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4789..6072)
                     /locus_tag="VYR08_000798"
     CDS             complement(4789..6072)
                     /locus_tag="VYR08_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV3466362.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6375..7088)
                     /gene="rsmG"
                     /locus_tag="VYR08_000799"
     CDS             complement(6375..7088)
                     /gene="rsmG"
                     /locus_tag="VYR08_000799"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV3466363.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7182..7742
                     /locus_tag="VYR08_000800"
     CDS             7182..7742
                     /locus_tag="VYR08_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV3466364.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7744..8643
                     /gene="htpX"
                     /locus_tag="VYR08_000801"
     CDS             7744..8643
                     /gene="htpX"
                     /locus_tag="VYR08_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV3466365.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8687..10246)
                     /locus_tag="VYR08_000802"
     CDS             complement(8687..10246)
                     /locus_tag="VYR08_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3466366.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEISNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10485..10802)
                     /locus_tag="VYR08_000803"
     CDS             complement(10485..10802)
                     /locus_tag="VYR08_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV3466367.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            10972..11514
                     /locus_tag="VYR08_000804"
     CDS             10972..11514
                     /locus_tag="VYR08_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV3466368.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVGDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11514..12143
                     /gene="nth"
                     /locus_tag="VYR08_000805"
     CDS             11514..12143
                     /gene="nth"
                     /locus_tag="VYR08_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV3466369.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12354..12875
                     /gene="pyrR"
                     /locus_tag="VYR08_000806"
     CDS             12354..12875
                     /gene="pyrR"
                     /locus_tag="VYR08_000806"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV3466370.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            12894..13817
                     /locus_tag="VYR08_000807"
     CDS             12894..13817
                     /locus_tag="VYR08_000807"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV3466371.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            13867..14946
                     /gene="carA"
                     /locus_tag="VYR08_000808"
     CDS             13867..14946
                     /gene="carA"
                     /locus_tag="VYR08_000808"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV3466372.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15258..18434
                     /gene="carB"
                     /locus_tag="VYR08_000809"
     CDS             15258..18434
                     /gene="carB"
                     /locus_tag="VYR08_000809"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV3466373.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTRLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAIAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5369 a         3577 c         4173 g         5354 t
ORIGIN      
        1 ttgaaaaatg atacttttac agtacccatg ttcggatggc atgggcaacg ccggattcgt
       61 catttgtttt ggtgatgtat ttggcgattt ttttgatttc tggatttcca ttttccatga
      121 caacggggtt tccaacgact tccagcatgg cacggtcatt ttcttcatca ccgattgcca
      181 tggtttcatc tttggtcaat ccgagttttt cagccaagtg agtaatggct gaacccttgt
      241 ctacattctt tttaaggagt tcgaggtaga aaggagcaga tttgttgatg gagtagcgct
      301 cgtaaaattc tgctggaatt ttttcaatcg cagcatcgag aatttctggt tcatcgataa
      361 acatacattt aacaatttct ttgccaacca tttcttcagg ggtacggtag aagataggca
      421 tgctgacgag ggttgattcg tgtacagtgt attttccgat attgcgattt gcagtataga
      481 taccgtcctt ggtaatggca tgcatgtgga caccgagctt gcgactgagg aattccatat
      541 ccagataatc ctcataggtc aaggattcgc tgataatctc atggccagta gcagtttctt
      601 ggacaagggc accattgaag gtcaccacat agtcaccttg gtctctcaac tgcaagtcgt
      661 ccagaagttt ggcaacacct gcgatggggc ggccagttgc aatcacgact ttgacaccag
      721 cttctttggc atcttggatg gcagaaaaga cttcaggagt gatttccttt tggctgttga
      781 ctagggttcc gtcaatatcg acggcaatta gtttaatact cataaaattc ctcttctagt
      841 ttttatttgg ggtaaaatga tcgttctcaa tcaagtgtaa aaattgctgg gtaatgctag
      901 caaagatact gttttggtct aacatttctt tagggaaata gaaacgatta tctccgtggc
      961 gactgccagc aagggattgg acgataagag acaggctaga gagttcggcc agttctccat
     1021 ttttttgtaa aatctcaatc tgtgtccgtg ggttttcaga ttcgggacga tagatatcat
     1081 aagggaggtc aaagttctta tgaatggcag tgtagtagtc gggatcaaag ccgatatcct
     1141 caaccaattt tctcatgcta gtaagttgat cttggtcctc ttgtgaaaag gtaatggatt
     1201 taaagacctt gcggttgaca aagcgatgcg ataaatctgc aagaatcttg tcaggactgg
     1261 tcatccaaag ctggaagtag gtattcatca cgccatcatc cagagccaga tagtcagtca
     1321 aggtcacatt tttttcgaag aaaggcagga ggtgtggaga agttcgggca aagaaatcct
     1381 tgtcctcagg atagagttcc ttggcgcgct tgagaagatt ctgtaggaga acttccatgg
     1441 cgcgtgttgc ggggtggaaa taaacctgca tgtacatctg gtagcgactg aggacgtagt
     1501 cttcgatggc gtgcatgcca ttgcgctgaa aggcgatacc attttcgata ggacgaatga
     1561 ctcggaggat gcgagtcagg tcaaattccc cataggatgc tcctgtaaaa taggagtcgc
     1621 gcaagagata gtccatgcga tctgcgtcaa tctgactaga aatgagctgc acgacctgct
     1681 tattaggata ggtatggtca atgacactgg ccaccttttc tgggaaatcg ggcgctactt
     1741 gtagcaagac ttggtgaatc tctgtctcag gattttgaat aatctcctga gtaatggctt
     1801 catggtctgt atcaaagaga tgttcaaaag tatgggagta ggcgccatgc ccaaggtcat
     1861 gtaggagagc agcggtcatg gtcaagagag actcggcagg attccattcc tcaggatatt
     1921 tttcttcaaa aatttctgtg atacgtcgtg caatctcata gactcccaga cagtgagaga
     1981 agcgactatg ctctccaccg tggaaggtat aactggaagt ccctagttgc ttgatccggc
     2041 gcaaacgttg aaattctttt gtattaatca agtcatagat gatttgatta ttgacatgga
     2101 tgtagttgtg aacagggtca cggaatactt tttcgttcat atgaccatta tagcaaaatc
     2161 ctgctctttt tggtaaaata gtaggacaag agacaagaaa gaggacctga tgtgaagatt
     2221 cggatgcgaa atacgattca gtttgatgag cagttggaag tgattgacca gctttatgat
     2281 gtggaagtgc atgaaaaggg agattatagc tatttactct tttataatga ggaaaaggaa
     2341 aaagtagtta ttaaatttca tggtcaagaa ctggtgatga gccgattttc taatcccaag
     2401 accattatgc gctttttaaa ggatagtgat agtttggcct atattcctac ccctatgggc
     2461 atgcaggaat ttatcattca aacgagccgt tatcaagttg ataggcaaaa gattgagcta
     2521 gcttatcaac tacaaaatca agaggggcat ccctttgcca gctatcaatt ggaaattact
     2581 tggggctagg atctcgtttt tctcaaatct actttctatc ttcaaggatt ggctccctag
     2641 tgtggtagat taggctattt tttggtataa taaaagttat ggaaatcgaa aaaaccaatc
     2701 gtatgaatgc gctctttgaa ttttatgcgg cgcttttgac agataagcaa atgaattata
     2761 tcgagctcta ctacgctgat gattacagcc ttgctgaaat tgccgaggag ttcggtgtca
     2821 gtcgtcaggc tgtctatgac aatatcaagc gaacagaaaa gattctggaa gattatgaga
     2881 tgaaattgca catgtactcg gactatattg tccgcagtca gatttttgat cagattttgg
     2941 agcgctatcc caaggataac tttctgcagg agcagataga aattttaaca agcattgata
     3001 atagagaata agaggaagaa aaatggcatt tgaaagttta acagaacgtt tgcagaacgt
     3061 ctttaaaaat ctacgtaaaa aaggaaaaat ctctgaatct gatgtccaag aggcaaccaa
     3121 agaaattcgc ttggccttgc tcgaggccga cgttgccttg cctgttgtaa aggactttat
     3181 caagaaagtt cgtgagcgtg cagtcgggca tgaggtcatt gatacactta atcctgcgca
     3241 acagattatt aaaatcgttg atgaggaact gacagccgtt ttaggttctg atacggcaga
     3301 aattatcaag tcacctaaga ttccaaccat catcatgatg gttggtttac aaggggctgg
     3361 taaaacaacc tttgctggta aattggccaa caaactcaag aaagaagaaa atgctcgtcc
     3421 tttgatgatt gcggcggata tttatcgtcc agctgccatt gaccagctta agaccttggg
     3481 acaacagatt gatgtgcctg tctttgcact tggaacagaa gtaccagctg ttgagattgt
     3541 acgtcaaggt ttggagcaag cccaaactaa tcataacgac tatgtcttga ttgatactgc
     3601 gggtcgtttg cagattgatg agctcctcat gaatgagctt cgtgatgtga aagcattggc
     3661 tcaaccaaat gaaatcttgc ttgtcgttga tgctatgatt ggtcaggaag cagccaatgt
     3721 tgcgcgtgag tttaatgctc agttggaagt gactggggtc atccttacca agattgatgg
     3781 cgatactcgt ggtggtgctg ctctgtctgt tcgtcacatt actggaaaac caatcaagtt
     3841 cactggtaca ggtgaaaaga ttacggacat tgaaaccttc cacccagacc gcatgtctag
     3901 ccgtatcctt ggtatggggg atatgctcac tttgattgag aaagcttctc aggaatacga
     3961 tgaacaaaaa gcccttgaaa tggctgagaa gatgcgcgaa aacacctttg attttaatga
     4021 tttcatcgat caattagatc aggtgcaaaa tatggggccg atggaagact tgctcaagat
     4081 gattccaggt atggccaaca atccagccct tcaaaacatg aaggtggatg aacgccagat
     4141 tgctcgtaaa cgtgccattg tgtcttcgat gacacctgaa gagcgtgaaa acccagattt
     4201 gttaaatcca agccgtcgcc gtcgtattgc tgctggttct ggaaatacat tcgtcgaagt
     4261 caataaattc atcaaggact ttaaccaggc taaacagctc atgcagggtg ttatgtctgg
     4321 ggatatgaat aaaatgatga agcaaatggg gattaatcca aataaccttc ctaaaaatat
     4381 gccaaatatg ggaggaatgg atatgtctgc ccttgaagga atgatgggac aaggcggtat
     4441 gcctgactta tcagctctcg gaggagcagg aatgccagat atgagccaga tgtttggtgg
     4501 cggtttgaaa ggtaaaattg gtgaatttgc catgaaacag tccatgaaac gtatggctaa
     4561 caaaatgaag aaagcgaaga agaaacgcaa gtaagacaag agattgatgc aaatctcatt
     4621 tgatgagaaa ataaagtaaa aatttctatg ataaaacgta cagtatcaag gttttagaat
     4681 atctgatatt atacgttttt tgattttaat aattttgtta agacactttt attatggtat
     4741 gcgaaaaatc cactcagctg tctgagtgga ttaggtgtat ttagactctt agtctttatt
     4801 ttcgtatggc aagatcaagt tcaagatgat tcctgtcatg gctgaaaggg cagtacctga
     4861 aagtgtaact ggaccaagtt taaggatagc tcctccaagt ccaagaacca acatagcact
     4921 tgcgatgatg aggtttcgca tttgagcgaa atcaacacgt tctttaatca agactttcaa
     4981 accattgctg gcgataaccc catagagaag gattgacata ccaccaagta cagcgtttgg
     5041 aatagttgaa atcaaggcag tgaatttacc aaggaagctg agggcaatcg cgatgaaggc
     5101 agcgttacgg ataactgaga cagaagcgat acgagtcata ccgataaccc ctgtattttc
     5161 tccgtaagtt gtattggctg gtccaccaag gaaggcagaa acagaagttg cgataccgtc
     5221 accaagaaga gtacggtgaa gacctggttc ttttaagaat tgacgaccac agatttgacc
     5281 caaaacagta tggtctccga tatgttcaga aattgttacg atagcgattg gcaagatagc
     5341 gatggcttct ggaccaaagt aaagattgta ctctttaaag gcaccacctg tgctaaatgg
     5401 caagtagaaa ccaggaattt cgaaccagtt ggctttaaga actggtgtaa agtcaaccaa
     5461 gccaagagtt agtgcgaaaa ggtaaccacc gataatggca aagaggaatg gaatgattcg
     5521 taggaagcct tttccttttg tattgataaa ggcagcaatt aggaaagtaa caacggctac
     5581 cagagcattt ttccaatttc cgtctgctac aagacctgca ttggtaacag ctgaacctgc
     5641 aagtccaaga ccgataacga tgatcatagg accgataatg attggtggca agagtttatc
     5701 aatccatttt gttcctacaa atcggatgct ggtagcaaca aggacataga ccaaaccagt
     5761 caagataacc cctgtttggg cagcagatac atcccccccc atttctttca tagccagtga
     5821 catagctgtg ataaaggcaa atgaagaacc tagataaact ggaactttaa aaccagtagc
     5881 aatcatgtag atgagtgttc caacacctga agcaaaaagg gcaacagata caggcattcc
     5941 caaaatcaat ggtaccaaga tggtcgcacc aaacatggcg aaaacgtgtt ggaaactaag
     6001 gagaattcct tttccatccg aaggacgttg gtcaacgtct agtaacaaat caacagttga
     6061 ttcttgtttc atataaacct cacttttggg catcagaaaa gagcccatta ttttacagca
     6121 ataggccctc agagtaagac ttctctaaaa tctagacttt aaagagtttc aaatatttct
     6181 gcgtcttcgt cacctcacgg gatgacatta aaaacctttg cttaggatag tatagcagaa
     6241 aaaaatgatt ttgtaaatca ttttttcccg agcctagaaa taaaagagcg aggctgattt
     6301 tgtaaatcat tttttcccga gcctagaaat aaaagagcga ggctgatttt gtaaacattt
     6361 gtttactaaa aaatttaaag tgggcgttta tttggcatac cagccttacg cggatattta
     6421 tttggtgttt cttttttctt ttctaccact gtgatatagc gcggatctct attcggtagg
     6481 gcgtagctga gattgtcttc gaccttacta aaaaggagat tgagggcatt cttagcttct
     6541 aataattcct caggcgcatt gctggccttg agtgccaata gttttccacc aaccttaagg
     6601 tagggaatgg tcaattcaga taggacctgc atacgggcaa ccgcacgagc tgttacaaaa
     6661 tcatattgag cacggaagtt cttgtcttgg gcaaaatctt cggcacgtcc gtggtagaaa
     6721 tgaactccgt tcaaatccag ttcttgagcc aagagttgta ggaagttgat gcgcttattg
     6781 agtgaatcaa taatggtcac atctaactcc ggatagagaa ttttcattgg tagactagga
     6841 aatcctgccc cagccccgat atcaagaagt ttgatagttt cattgggaat caaaccttga
     6901 agaatgggtg caatcgaatc gtaaaaatgt ttgagataaa cttcttcctt gtccgtaatc
     6961 gccgtcaaat taatcttctc attccactcg accaagagct caaaataacg ttcaaattgt
     7021 tctttttgct ggtccgaaag tggaagattc tgctcggcaa gcaagttgta aaatgttttt
     7081 ggtttcatag ttatttcctt ctttagtatc atcttatttt accacaatca tagaaaattt
     7141 gatatactgg tactaatcta aaagcagaaa ggacttatat tatgacttgg attattcttg
     7201 gagttatcgc tcttattgtt atttttgtga ttgttagcta taacggtttg gttaaaaatc
     7261 gtatgcaaac caaggaggct tggagtcaga ttgatgttca gttgaaacgt cgaaatgacc
     7321 tcttgccaaa cttgattgag actgtaaaag gttatgccaa atatgaaggt tctacccttg
     7381 aaaaggtggc agaactacgt aaccaagtgg cggcagcgac ttcaccagca gaagctatga
     7441 aagccagtga tgccctcact cgtcaggttt caggtatttt tgcagttgca gaaagctatc
     7501 cagatttgaa agctagtgct aactttgtta aattgcaaga ggagttgaca aacacagaaa
     7561 ataaaatttc ttactctcgt caactctata acagtgttgt cagcaactac aatgtaaaat
     7621 tagaaacttt cccgagcaat attatcgctg gaatgtttgg atttaaagcg gcagatttcc
     7681 ttcaaacacc tgaagaggaa aagtcggttc ctaaagttga ttttagcggt ttaggtgact
     7741 aagatgttgt ttgatcaaat tgcaagcaat aaacgaaaaa cctggatttt gttgctggta
     7801 tttttcctac tcttagctct tgttggttat gcggttggtt atctctttat aagatctgga
     7861 cttggtggtt tggttattgc actgattatc ggctttatct acgctttgtc tatgattttt
     7921 caatcgacag agattgtcat gtccatgaat ggagcgcgtg aggtggatga gcaaacggca
     7981 ccagacctct accatgtagt ggaagatatg gctctggtcg ctcagattcc tatgccccgt
     8041 gttttcatca ttgatgatcc agccttaaat gcctttgcga caggttctaa tcctcaaaat
     8101 gcggctgttg ctgcgacttc aggtctacta gctatcatga atcgtgaaga actagaagct
     8161 gttatgggac atgaagtcag tcatattcgt aattatgata tccgtatttc gactattgca
     8221 gttgcccttg ctagtgctat caccatgctt tctagtatgg caggtcgtat gatgtggtgg
     8281 ggtggagcag gtcgcagacg aagtgatgat gaccgagatg gaaatggtct tgaaatcatt
     8341 atgctagtgg tttccctact agctattgta ctggcacctc tcgctgcaac cttggttcag
     8401 ctcgctattt ctcgtcagag ggaatttctg gcagatgcat ctagtgtcga gctgactcgc
     8461 aatccccagg gaatgattaa tgccctagat aagttggaca atagcaaacc tatgagtcgc
     8521 cacgtcgatg atgctagcag tgccctttat atcaatgatc ctaagaaagg tggggggttc
     8581 caaaaactct tttataccca cccacctatc tcagaacgga ttgaacgttt aaaacagatg
     8641 taaaagaatc cagaggtcgt ctctggattt ttgctatgtt gcaaatttat aaatcttcta
     8701 aattaactag cttcaaaccg tgttctgtca tgcgatagat gatcgagcag acttctttta
     8761 agaaacgacg gtcatgcgaa acagtgatga gaccgcctgg ataggttgca aagagttttc
     8821 tgatttgggg ttgagaagtg ggagaaaagt ttcgtgtggg ttcatccagc aggagaaagt
     8881 ttggtttgcg caggactaga tccaaaagca ggagttttcc ctgttgtccg ccagataagg
     8941 agcgaatttg atgctgcatt tctggataac tgaaattgag actggctagg tgagattgga
     9001 ttttctgaag ttcctctttt tccccagttt tgctgatata agctattggt gataaatcca
     9061 attgcagttt tttgtggtaa tcttgtggca taaaaccaag ggaaatctct cttttatcat
     9121 tcagaagttg ctttaatttg gctaacagag ttgatttccc aacaccattt ggcccgataa
     9181 taccgatttt ttcttgccca cggacagtta gttgtagttt ttgaaccaaa actcggtcgt
     9241 caatggacaa attttctttt tccagttgga ctaagacttt agaagctggt aatggttgga
     9301 tgtctgaaaa gaaaagttgg atttgttcct cttcaagtgg cttttgagtc atggactgag
     9361 ctgccttttc gtagcgtttt tcttgtgaga ggacagtttt catcttttta gccaataggc
     9421 gaccggcagt actatctttg gtagctcgaa gcgcagtttc tacattttgc ttaactctcc
     9481 gatgtttttc catggtttta tcgtaggctc tttggttgtt agcagcttgc tgactttgtt
     9541 tggcaaaatt agcctttctc tgctcactat agctatcata gtctaaatgc tctactagcg
     9601 tttccgcttc tttacggtgt ttgaccagtc gcaagtgaac aatagtgtct gccgtttcag
     9661 aaagaaagtc ttcatcatgg gaaatgaaaa taacggtttg tctggtcttt tgaatctggc
     9721 cttttagcca atcaactgtc tcaaggtcta ggtcatttga aggttcatct aaaaatagaa
     9781 tctcaaaggg tttggctaac tcatggataa gctgaatttt caaagcttct ccccctgata
     9841 gattgctaat ctcttggtca cttgcgaaac gattgctatc aaaatgcaat tcctccgcca
     9901 aacgatagag gatactgtag tctaaatcaa tagaatctaa aaagaagtag tcgtgtaaag
     9961 ttttcttttt cagctcctcg ggaagttttt gaggaatgta ggccagtgac tgatagtcag
    10021 attggatgtt tcccttgata gtgaaatcag acaaagcttc ccccattaaa attttaagta
    10081 aggttgattt gccatttcct tcttcaccaa taatagcaac cttttccccg tcttggatgg
    10141 tcatggttag gtcagataca agatctcgta aatctttgtt ttgtgtgatg gttagatgat
    10201 tgatttttat catatgattt ccctttctag aatgttcata gtgcataaca aaaagctcta
    10261 ctttacctag tagagcccaa agtctatgtc aaaaaaactc tattaacatg ccagaaaaag
    10321 agcataaaag agcacacaaa aagagacaaa aacaagatct actaaataaa ggttctttat
    10381 ttgatagact tagttgttca tgtctccctt acctccgagt attagtatct ttatattata
    10441 cctttaagga aattttgtca acagaaaact ggaaattcta tgctctaaaa ttcaaaaaca
    10501 ggtccataga gacttagtac gtgtttgaca tgctcgtaat ggaatttatc atagtttcgc
    10561 caagcagtgc gtgcttgatt gtttagtttt aggctaccca gtccaatcag aggactggtg
    10621 ggttggtaaa atttctcaag atcaattaag atactgttgt tcaacttttc cgtttttttg
    10681 agctcttgca tagtagtgtt tagcagctct tgtagtcgaa tatcatctgc cgtaccctgt
    10741 ttacagtttt ggtagctttt atttaacttg gataggagtt gaatgagttt cttgtcttcc
    10801 atgtgagcat cctccttgat ctgatgtact ctttccctat agtatagcac taaaaaacaa
    10861 aactgtcata gtaaatatgt taaaattttg aattttaatt acaaagtttg gtgtgctgat
    10921 ttgtagcctt ttcatggtat aatcaagaga gtaaatcttt atggaggaag tatgaaatta
    10981 aatattcaag aaattcgtaa gcagtctgaa ggtttgaact ttgaacaaac gttagattta
    11041 gttggtgacc tgcgtgcacg taatcaagaa attttagatg taaaagatat ccttgcagtt
    11101 gggaaagtac aatatgaaga ccgtatgtat ttcttagatt atcaactatc ttataccatt
    11161 gttcttgctt cgagtcgcag tatggagcca gttgagttag ttgaatctta tccagtcacg
    11221 gaagttttca tggaaggcgc aactaaccag ctagatcaag aagttttaga tgatgacttg
    11281 gtcttgccca tcgaaaatgg ggagcttgac cttgctgaga gtgtatcaga caatatcctg
    11341 ctaaacattc ctatcaaggt cttgacggct gaagaagaag ctggtcaagg atttatctca
    11401 ggaaatgact ggcaaatcat gacagaggaa gaataccaag ctcaaaaagc agtaaagaaa
    11461 gaagaaaaca gtccttttgc tggcttacaa ggactatttg acggagatga ataatggtct
    11521 tgtcaaaaaa acgagcacga aaggtgctag aagaaatcat tgccctcttc ccagatgcca
    11581 agcctagtct tgattttacc aatcattttg aactcctggt tgcggtcatg ttatcagccc
    11641 agacgacaga tgcagcggta aataaggcca caccaggtct ctttgttgcc tttccaacac
    11701 cccaagctat gtctgtagcg acagagagtg agattgcttc acatatttct cgcttgggat
    11761 tgtatcggaa taaagctaaa ttccttaaaa aatgtgccca acagttacta gacgattttg
    11821 atggtcaagt ccctcagaca cgtgaagaat tggagagttt ggcaggtgtt ggtcgcaaga
    11881 cagccaatgt tgtcatgagt gtaggatttg ggattccagc ctttgcagtg gatactcatg
    11941 tggagcgtat ttgcaaacac cacgatattg tcaaaaaatc agcgacgcca cttgaggtgg
    12001 aaaagcgggt catggatatc ttgccgcctg agcagtggtt agctgcccat caggccatga
    12061 tttattttgg aagagccatt tgccatccaa aaaatccaga gtgtgaccag tacccacaat
    12121 tatatgattt tagcaatttg taagatgcta cattgaaact ttttgcttga ttttaagcat
    12181 gctttgtgct atagtaaatg ataactaaat attcctttaa acctgtcccg tgaggcaggc
    12241 aaggagcgcg ataaagtaga agggtgaagt ctatactgtg tgcagtaggg ctcgcttggc
    12301 tataaatttc aagtctcctt gtttaaggag acttttcttt ttggaggttt gtcatgaaaa
    12361 caaaagaagt tgtagacgaa ttgaccgtca aacgagcgat tacgcgtatt acttatgaga
    12421 ttatcgaacg caacaaagat ttgaataaga tcgtcttggc tggtattaaa actcgtggtg
    12481 tctttattgc ccaccgaatc caagaacgtt tgaagcagtt agaaaatctt tcagttcctg
    12541 ttgtggaatt ggatactaaa cctttccgtg atgatgttaa aagtggagaa gatacttctt
    12601 tggtttctgt cgatgtgaca gaccgcgaag ttatcttggt ggatgatgtg ctctatacag
    12661 gtcgtaccat ccgtgctgct attgataata ttgtcggtca tggtcgtcct gcgcgtgtga
    12721 gtttagcagt tctagtcgat cgtggacata gagaattgcc aatccgtcca gattacgttg
    12781 gaaaaaatat cccaaccagt cgttctgaag aaatcatcgt agagatgaca gaacttgatg
    12841 accaagacag agttctgatt actgaagaag cttagaaagt taaaggagta gccatgtcag
    12901 aaaatcaaca agcattgaac catgtggtgt ccatggaaga cctcactgtc gatcaagtaa
    12961 tgaaattgat caagcgagga attgagttta aaaatggagc ccagcttccc tatgaagacc
    13021 atccgattgt ttccaatctc ttctttgagg attctacacg gacacataag tcctttgaag
    13081 tcgcagagat taaacttgga ttggaacgac ttgactttga tgtgaagact agttcggtta
    13141 ataagggtga gacactttat gacaccattt tgactctgtc tgctttagga gtggatgtct
    13201 gtgtgattcg ccatccagag gtcgactact atagagaatt gattgctagt ccaacgatta
    13261 cgacttccat catcaatggt ggagatggtt cgggccaaca ccctagccag agcttgcttg
    13321 atttgatgac catttatgag gaatttggcc actttgaggg tcttaaagtt gctattgcag
    13381 gtgacttgga ccactcacgc gttgccaaat ccaatatgca gattttgaaa cgcttgggag
    13441 ctgaactctt tttcgctgga cctgaggaat ggagaagtca agagtttgca gactatggaa
    13501 agtttgtaac cattgatgaa atcattgatc aggttgatgt catgatgttt ctccgtgtgc
    13561 aacacgaacg ccatgatagt ggagctgtat tttcaaaaga agactaccat gcccaacatg
    13621 gcttgactca agaacgttac gatcgtttga aagaaacagc aatcctcatg cacccagctc
    13681 caatcaatcg tgatgtagaa atagcagacc acttggttga agcaccaaaa tcacggattg
    13741 tccaacaaat gaccaatggt gtctttgttc gaatggcaat cttagaatcc gtactagcga
    13801 gtagaaacgc caactaaaac acaagacgtt aaaagacgcc agtgagcgtc cacgagaggt
    13861 gaaaatatga caaaaagaat tctagtatta gaagatggca cagtttttga aggcaaggcc
    13921 ttcggagcag atattgatgt aacaggcgaa atcgtcttta atacagggat gaccggctac
    13981 caagaatcca ttacagacca gtcttataat ggacaaatct tgacctttac ttatcctttg
    14041 gtaggaaatt atggtattaa tcgtgatgat tatgaatcca ttattccaac ttgtaaggga
    14101 gtcgttgttt ttgaagaagc gcgtagagct agcaactggc gcaaccaaat gaccttggat
    14161 gaatttttga aagccaagaa aattccaggg atttcaggaa ttgatacgcg tgctcttacc
    14221 aagattatcc gtaagcatgg tactatgcgt gcaaccttga cccatgttgg ggacagtatg
    14281 gaccatgtga cggaccagct ccaagcaaca gttttaccga cagacaatat caaacaggtt
    14341 tctactaaaa cgtcttatcc agctccagga gttggtttga gcgtggtgct agtggacttt
    14401 ggtctcaagc actcaatcct acgtgaactt tctaaacgca actgtaacgt gacagttgtt
    14461 ccttattcga caacggctga agaaattctc catctcaatc ctgatggagt tatgttgtca
    14521 aatggtccag gtaacccaga agacgttcca caagcactcg acatgattcg cggtgtacaa
    14581 ggaaaaattc caatctttgg tatttgtatg ggacaccaac tctttgcaat ggcaaacggg
    14641 gctaagacct ataagatgaa gtttggccac cgtggattca accatgcggt acgtgaaatc
    14701 gcaacaggac gagtagactt caccagccag aaccatggtt atgcagtcag ccgcgaggat
    14761 ttgccagagc acttgattat tacccacgaa gaaatcaatg acaagtcagt ggaaggtgtg
    14821 cgccacagat accaacctgc tttctctgtt caataccacc cagatgcagc tcctgggcca
    14881 cacgacgcta gctacctatt tgacgagttt atcgagatga tggaagtttt taaacaatca
    14941 aactaagaac gagtaaaagc tcgtcggaga atttatgtaa ctgaacacgg acggaaagct
    15001 cagaatgtag agaaaccaac ttggattgtc aatccttcct tgattttcta aaattcaatc
    15061 gctttctatt cgtcctttgt atcttattta atgtcgccct gagaggcgac gaatagaaag
    15121 gagaagatac attgttcgcg gaagtgaaca cgccctaaga actgtgtgaa aaagataaac
    15181 atcgtcttga cgctgaaggc gtctgcgccg agtttcctat tttcactgtg ttctttacgg
    15241 gctttgtatc ataaactatg cctaaacgta ctgatattca aaaaattatg gtgattggtt
    15301 ctggtccgat tattattggt caggctgctg agtttgacta cgctgggacc caggcttgct
    15361 tgtcgttgaa agaggaaggt tatgaggttg ttttggttaa ctcaaatcct gcaaccatca
    15421 tgacggacaa ggaaattgct gataaggttt acattgaacc gattacactt gagtttgtga
    15481 cacgtattct tcgtaaggaa cgtccagatg ccttgctacc aacactcggt ggtcagacag
    15541 gtctcaacat ggccatggaa ttgtctaaaa atggtatctt agatgagctt ggtgtagagc
    15601 ttctcggtac tagattgtct gccattgacc aagcggagga ccgtgacctc tttaaacaat
    15661 tgatggaaga gcttgagcaa cccattccag aatctgaaat tgttaacaca gtagaagaag
    15721 caattgcctt tgcagcgaca attggctacc cagttatcgt tcgtccagcc tttacccttg
    15781 gtggtactgg tggtggtatg tgtgccaacg agaaagaatt gcgtgaaatc actgaaaatg
    15841 gtttgaaatt gtcacctgtt acccaatgtt tgattgagcg ttcgattgcc ggtttcaagg
    15901 aaattgaata cgaagtgatg cgtgactcag ctgacaatgc cttggttgtt tgtaacatgg
    15961 aaaactttga cccagttggg attcacacag gggattccat tgtatttgcc cctgcgcaaa
    16021 ccatgtcaga ctatgaaaac caaatgcttc gtgatgcgag cttgagcatt attcgtgccc
    16081 tcaagattga aggtggatgt aatgttcagc tagctcttga tcctaacagc ttcaagtatt
    16141 atgttatcga agtaaaccct cgtgtatcac gttcgtcagc ccttgcttct aaagcgacag
    16201 gatatccgat tgctaagttg gctgccaaga ttgcagtagg tttgaccttg gatgaggtta
    16261 taaacccagt tacaggttca acctatgcca tgtttgaacc agcccttgac tacgtagttg
    16321 ctaagattcc acgtttccca tttgacaagt ttgaaaaggg tgaacgccgt ctcggtacac
    16381 aaatgaaggc gactggagaa gtcatggcaa tcggtcgtaa catcgaggaa tctcttctca
    16441 aggcctgccg ctcccttgaa attggggtgc accacaatga aatacctgaa cttgcagcag
    16501 tttctgatga tgccttgatt gaaaaggttg tcaaagccca agatgaccgt ctcttctatg
    16561 tgtcagaagc cattcgccgt ggttacacac ctgaagaaat tgcagagctt acaaaaattg
    16621 atatcttcta tcttgataaa ctcttgcata tctttgaaat tgagcaagaa ttgggtgctc
    16681 atccacaaga tctagaagtt ttgaaaacag ccaaacttaa tggtttttca gaccgtaaga
    16741 ttgctgaact ctggggaacg actgatgata aagttcgcca acttcgcttg gaaaacaaga
    16801 ttgttccagt ttataagatg gtagacactt gtgcggcaga gttcgactcc gaaacgccat
    16861 atttctattc aacctatggt tgggaaaatg aatctatcag atctgataaa gaatctgtac
    16921 ttgtcctagg ttcaggtcca atccgtatcg gtcaaggggt tgagtttgac tacgcaaccg
    16981 ttcactctgt taaggctatc caggcagctg gttatgaagc tatcatcatg aactcaaacc
    17041 cagagaccgt ttcaacagac ttctctgtat cagataagct ttactttgag ccattgacat
    17101 tcgaagatgt tatgaacgtc attgacttgg aacaaccaaa gggagttatc gttcagttcg
    17161 gtggtcaaac agccatcaac cttgcggagc cattggcaaa agcaggtgtg accatccttg
    17221 gtacacaggt tgctgaccta gaccgtgccg aagaccgtga cctcttcgag caagctctca
    17281 aagaattgga tattccacag ccaccaggac aaacggctac caatgaagaa gaagcagctc
    17341 ttgcagctcg taagattggc ttcccagttc ttgtccgccc atcttatgta cttggtggac
    17401 gtgccatgga aatcgttgaa aacgaagaag accttcgttc ttacatgcga actgctgtta
    17461 aggctagtcc agaccaccca gttcttgtcg actcttatat cgttgggcaa gagtgcgaag
    17521 ttgatgccat ttcagatggt aaaaatgtac tcattcctgg tatcatggag catatcgaac
    17581 gtgctggtgt ccactcaggt gactcaatgg ctgtttaccc accacaaacc ttgtcgcaaa
    17641 aggttcagga gaccatcgca gactacacca aacgcttagc aatcggtctt cactgccttg
    17701 gaatgatgaa catccagttt gttatcaagg atgaaaaagt ctacgttatt gaggtcaatc
    17761 cacgtgccag ccgtaccgtt ccattcctat ctaaggtaac caatattcct atggctcaag
    17821 tagcgaccaa gctcattctt ggtcaaagtt tatcagaact tggctaccaa aatggacttt
    17881 accctgaaag cacccgtgtt catatcaagg cacctgtctt ctcctttacc aaactagcta
    17941 aggtagacag cttgctcggt cctgaaatga agtcaacagg tgaagttatg ggttctgatg
    18001 ctactttgga aaaagctctc tataaagcct ttgaagcttc ttatctacac ttgccaactt
    18061 ttggtaatgt cgtctttacc atcgctgatg atgccaaaga agaagccttg aacttggctc
    18121 gtcgtttcca aaacattggt tatggaatcc ttgcgacaga agggacagca gccttctttg
    18181 ctagtcatgg cttgcaagcc caacctgttg gtaagattgg tgatgacgat aaggatatcc
    18241 caagttttgt acgcaaagga agaattcaag ctatcattaa cacagtagga acaaaacgaa
    18301 ctgctgacga agatggtgag caaattcgtc gttcagccat tgaacacggg gtgcccctct
    18361 tcacagcgct agatacagct aatgctatgc ttaaggtact tgaaagtcgt agctttgtta
    18421 cagaagcgat ttaaatagaa aaataagata tagcaaactg aatttagaac agt
//
LOCUS       DAVMHG010000030        18130 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMHG010000030 DAVMHG010000000
VERSION     DAVMHG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.030"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            61..1953
                     /gene="pepO"
                     /locus_tag="VYR08_000810"
     CDS             61..1953
                     /gene="pepO"
                     /locus_tag="VYR08_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV3466374.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2076..2705)
                     /locus_tag="VYR08_000811"
     CDS             complement(2076..2705)
                     /locus_tag="VYR08_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3466375.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3701..5923
                     /locus_tag="VYR08_000812"
     CDS             3701..5923
                     /locus_tag="VYR08_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV3466376.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5953..6396
                     /locus_tag="VYR08_000813"
     CDS             5953..6396
                     /locus_tag="VYR08_000813"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV3466377.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6435..6850)
                     /locus_tag="VYR08_000814"
                     /pseudo
     CDS             complement(6435..6850)
                     /locus_tag="VYR08_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6869..7282)
                     /locus_tag="VYR08_000815"
     CDS             complement(6869..7282)
                     /locus_tag="VYR08_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466378.1"
                     /translation="MKKLYRIHFIAIAAIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(7413..8047)
                     /locus_tag="VYR08_000816"
                     /pseudo
     CDS             complement(7413..8047)
                     /locus_tag="VYR08_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(8235..8885)
                     /locus_tag="VYR08_000817"
     CDS             complement(8235..8885)
                     /locus_tag="VYR08_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV3466379.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            8997..9839
                     /locus_tag="VYR08_000818"
     CDS             8997..9839
                     /locus_tag="VYR08_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV3466380.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9915..10352
                     /locus_tag="VYR08_000819"
     CDS             9915..10352
                     /locus_tag="VYR08_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV3466381.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10420..10722
                     /locus_tag="VYR08_000820"
     CDS             10420..10722
                     /locus_tag="VYR08_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466382.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10813..11886)
                     /locus_tag="VYR08_000821"
     CDS             complement(10813..11886)
                     /locus_tag="VYR08_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV3466383.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            12042..12719
                     /locus_tag="VYR08_000822"
     CDS             12042..12719
                     /locus_tag="VYR08_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466384.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12712..13686
                     /locus_tag="VYR08_000823"
     CDS             12712..13686
                     /locus_tag="VYR08_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3466385.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13735..15678)
                     /gene="thrS"
                     /locus_tag="VYR08_000824"
     CDS             complement(13735..15678)
                     /gene="thrS"
                     /locus_tag="VYR08_000824"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV3466386.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEARKIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15736..15912)
                     /locus_tag="VYR08_000825"
                     /pseudo
     CDS             complement(15736..15912)
                     /locus_tag="VYR08_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            complement(16490..16690)
                     /locus_tag="VYR08_000826"
     CDS             complement(16490..16690)
                     /locus_tag="VYR08_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466387.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            16929..17558
                     /locus_tag="VYR08_000827"
     CDS             16929..17558
                     /locus_tag="VYR08_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3466388.1"
                     /translation="MKLAVIAANGQVGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17699..17968
                     /gene="rpsO"
                     /locus_tag="VYR08_000828"
     CDS             17699..17968
                     /gene="rpsO"
                     /locus_tag="VYR08_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV3466389.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
BASE COUNT         5344 a         3402 c         4152 g         5232 t
ORIGIN      
        1 ttgcattccc tagtgatttt tgttaagata aaggcaaata caaatgaaag cgagaacaag
       61 atgacacgtt atcaagatga tttttatgat gctatcaatg gagaatggca acagacagct
      121 gaaatcccag cagataagtc tcaaacagga ggttttgttg atttagacca ggaaattgaa
      181 gacttgatgc tggcgacaac agacaagtgg ttagcaggtg aagaagtgcc tgaggatgct
      241 atcttggaaa actttgtcaa ataccaccgc ctagttcgtg attttgacaa gagagaagct
      301 gacggtatca cacctatctt atcactcctt aaagaatttc aagaattgga gacttttgcg
      361 gattttatag ctaaactagc agagtttgag cttgcaggaa aaccaaactt ccttcctttt
      421 ggtgtatcgc cagactttat ggatgctaga atcaatgttc tatgggctag cgctccaagc
      481 acaatcttgc cagatacgac ctactatgca gaagaacatc ctcagcgcga agagctcttg
      541 actctttgga aagaaagcag cgcaaatctc ctcaaggctt atgatttctc tgatgaagaa
      601 attgaagact tgctagaaaa aagacttgaa ttggaccgcc gagttgcggc agtggtgctc
      661 tctaatgaag aaagttcaga atatgctaaa ctctatcatc catattctta cgaagatttc
      721 aagaaattcg cgcctgccct acctttggat gacttcttca aagcagttat tgggcaatta
      781 ccagacaagg ttattgtaga cgaggaacgt ttctggcaag cagcagagca attctacagt
      841 gaggaagcct ggtctctcct taaagcaacc ttgattttga gtgttgtcaa tctttcaacc
      901 agctatttaa cagaggatat ccgtgttttg tctggtgcct acagccgtgc cctttctgga
      961 gttccagagg caaaagataa ggtcaaagca gcttatcatc tagcacaaga acctttcaag
     1021 caagccctgg gtctttggta cgcccgtgag aagttctctc cagaagccaa ggcggatgtg
     1081 gagaaaaaag tggcaaccat gattgatgtc tataaggagc gtctgcttaa gaatgactgg
     1141 ctcactccag aaacctgtaa acaggctatc gtgaagctca atgtgatcaa accttatatt
     1201 ggctatccag aagaattgcc tgcacgttac aaggataagg tagtgaatga aactgccagt
     1261 ctttttgaga atgctctagc ctttgcgcgt gtggaaatca agcacagttg gagtaagtgg
     1321 aaccagcctg tagattataa ggaatggggc atgcctgctc atatggtcaa tgcctactac
     1381 aatcctcaga agaacctgat tgtctttcca gcggccattt tacaggcgcc tttctatgac
     1441 ttgcatcagt catcttctgc taactacggt ggtattgggg cagtgattgc ccatgaaatt
     1501 tcccacgcct ttgatactaa cggggcttcc tttgacgaaa atggtagcct caaggattgg
     1561 tggacagaga gcgactatgc tgccttcaag gagaaaacac aaaaagtcat tgaccaattt
     1621 gatggacagg attcttatgg agcaaccatt aacggtaaat tgactgtatc agaaaacgtg
     1681 gctgacttgg gaggaatcgc agcagcgctt gaagcagcta agagagaagc agacttctca
     1741 gcagaagagt tcttctacaa cttcggtcgc atctggcgca tgaaaggtcg accagaattt
     1801 atgaaacttt tggctagcgt cgatgtgcac gcaccagcca aactccgtgt caatgtgcaa
     1861 gtaccaaact tcgacgattt ctttacaacc tatgatgtca aagaaggaga cggaatgtgg
     1921 cgttcaccag aggagcgcgt gattatttgg taatacaaaa aatctagtca tcagaaaaaa
     1981 ggcatccaat cacgtgtgaa aaccttgata ggatgccttt tgtaatagaa agatttgctg
     2041 gatagtttac ttaaatttcg attgtcatca tcttgctaga aaaagggatt gaaggccttt
     2101 tcgtgagcga tagtagtagc tggaccatgt cctggataga catcgtagtt tggtagggtg
     2161 aagagttggg tctggatact atgaaggagt tgctccatgc taccagtcgg aaggtcggtc
     2221 cgtccgatag tttcgcggaa tagagcatct cccgtcaaga ctaggtgagc atcaggaaag
     2281 actagggaaa caccaccgat agagtgccct ggggtcggta gaaccttaaa acgaaattcc
     2341 tctagttggt attcttcgtg aaagacaaag gtgtgttctg caggttttgt gaccacatct
     2401 gccatatcat cgtggcgagg gagaccggag agattatcga caggagtgta gagccagctg
     2461 gcttcgctct ctgcgatata gacaggagga ttgccaaacg tctcgcgaac caagtccaga
     2521 ctcatgatat ggtcataatg ggcgtgggtc aagagaatag cacagatcgg tttgttgatc
     2581 ttctcgattg tctgacgaat ggcttcccaa tgactaccag gatcgacgac gatgaggtgc
     2641 ttttcgcctt ctagataata ggtattttca taggcaacag gattcacggt tttatggatt
     2701 ttcatactag ctccaatctc aaagaatgta ctaatactct tcgaaaatct cttcaaaccg
     2761 cgtcaacttt atctgcaatc tcaaagcagt gctttgaaca gcctgcggct agctttctag
     2821 tttgctcttt gattttcatt gagtataaat taagtatagc acagttaggg agaataggta
     2881 aggatttaaa atgaacttaa aaaccagcat gactagatga agacaggctg gttatcaatt
     2941 tatcaatatt ggaaaggtgt caattgccct tcataggttg cataaactcc gtcattagct
     3001 tgtttgatag acgtgatgtt tagagtgcta ccgtagttgg cttctcattt gtcgctatat
     3061 tcactataag ttatcctact gggcaggttc tggtcgttag catagtattg aacgactgtt
     3121 ttttatagtt ttgctggctg aataggaaca ggcagcaaac tcctaacatt aggagagcaa
     3181 agatagaaat agcagttttt ttcataggta ttctccttat aaactaaagg atagaagaaa
     3241 gtatcttgag gatttttata ctcttcaaaa atctcttcaa accgcgtcaa cgtcgccttg
     3301 ccgtatatat gtgactgact ttgtcagtct tatctacaac ctcaaagcag tgctttgaac
     3361 agcctgcggc tagtttccta gtttgctctt ggattttcat tgagtattaa ttccaaaatc
     3421 cagtcaaggt accagtgtat gtgatacgag ttccaccgtt tatctttcgt atcttcaatg
     3481 gaattgggga agtcagaaag tctttgggtt acaatatggt ttccgttctt ctatctctcc
     3541 ttctatcacc taagtttaaa atctaacttc gtagtatatt atggaccaat agttcagaat
     3601 tatcaagcaa ttttgaatta tttttttctt attctgttcc aatctagttt tcatattctt
     3661 caaaaagtga taaaatggta ggaagaattg gagagtagag atgccgaaag aagtgaattt
     3721 aacaggcgaa gaagttgtcg ctttaaccaa agaatattta acggaagagg atgttcattt
     3781 tgtccataag gccttggtct atgctgttga atgccacagt ggtcaatatc gcaaatcagg
     3841 cgagccttat atcattcacc ctatccaagt ggcaggtatt ttagctaagc taaagctgga
     3901 tgctgtaaca gtagcttgtg gattcttgca tgatgtggtg gaagatacag atgcgacctt
     3961 ggacgatttg gaaagagagt ttggtcctga tgtgcgggtg attgttgacg gagttaccaa
     4021 gcttggcaag gtcgagtaca aatcgatcga ggagcaatta gcggaaaatc atcgcaagat
     4081 gctcatggcc atgtctgagg acatccgcgt tattttggtc aaactgtctg accgcttgca
     4141 caatatgcgg accctgaaac atcttcgaaa agacaagcag gagcgtattt ccaaagaaac
     4201 catggaaatc tatgccccac ttgcccatcg tttggggatt tccagtgtca aatgggaatt
     4261 agaagacttg tctttccgtt atctcaatcc aacggagttt tacaagatta cccatatgat
     4321 gaaggaaaag cgcagggagc gtgaggcctt ggtggatgag gtagtcacaa aattagagga
     4381 gtatacgaca gaacgtcact tgaaagggaa gatttatggt cgtcccaagc atatttactc
     4441 aattttccgc aaaatgcagg acaagagaaa acggtttgag gaaatctatg atctgattgc
     4501 tattcgttgt attttagata cccaaagtga tgtttatgcc atgcttggtt acgtgcatga
     4561 attttggaaa ccgatgccag gtcgcttcaa agactatatt gccaaccgca aggccaatgg
     4621 ttatcagtct atccatacga ctgtttatgg accaaaaggg ccgattgaat tccagattcg
     4681 aaccaaggaa atgcacgagg tggctgagta cggggttgcg gctcactggg cttataagaa
     4741 aggtataaag gggcaagtta acagcaagga atcagctatt ggaatgaact ggatcaagga
     4801 gatgatggag ctccaagacc aggctgatga tgctaaggaa tttgtggact ctgttaagga
     4861 aaactatctg gctgaggaga tttacgtttt taccccagat ggagctgtcc gttcccttcc
     4921 caaagattca ggaccgattg attttgccta cgaaatccat accaaggtcg gtgaaaaagc
     4981 aactggtgcc aaggtcaatg gccgcatggt tccactgaca accaagttaa agacagggga
     5041 tcaggttgaa attatcgcca acccgaactc ctttggacct agccgtgact ggctcaatat
     5101 ggtcaagact agcaaggcgc gcaataagat tcgccagttc tttaaaaacc aagataagga
     5161 attgtctgtc aacaagggtc gtgagatgct gatggctcag ttccaagaaa atggctatgt
     5221 ggcaaataaa tttatggaca agcgccacat ggatcaagtt ctgcaaaaga ccagttacaa
     5281 gacagaagac tccctctttg cggccattgg ttttggggaa atcggtgcga ttaccgtctt
     5341 taaccgtctg actgaaaagg aacgccgtga ggaagagcgt gccaaggcca aggctgaggc
     5401 agaggagctt gtcaaaggtg gcgaggtcaa ggttgaaaat aaagaaaccc tcaaggtcaa
     5461 gcatgagggg ggagtggtta ttgaaggtgc ttctggtctc ctagtgcgga ttgctaagtg
     5521 ttgtaacccc gtgcctggtg acgatattgt tggctacatt accaagggtc gtggtgtggc
     5581 tattcaccgt gtggactgta tgaacctgcg tgcccaagaa aactacgagc aacgtctcct
     5641 tgatgtggaa tgggaagacc agtactctag ctcaaataag gagtatatgg cccatatcga
     5701 tatctacggt ctcaaccgta caggactgtt gaacgatgta ctgcaagttc tttcaaatac
     5761 aaccaagaat atttcaacgg tcaatgccca accaaccaag gatatgaagt ttgctaatat
     5821 ccatgtgtcc ttcggtattg ccaacctctc tacactgacc acggttgtcg ataaaattaa
     5881 gagtgtgcca gaagtttact ctgtcaaacg gaccaacggc tagttgtcct agctcttact
     5941 agaaaggcta ttatgaaaat cattatccaa cgggttaaaa aagcccaagt gagtatagaa
     6001 ggccagattc agggaaaaat caatcaggga cttttattgc tggttggtgt tggaccagag
     6061 gaccaagagg aagatttgga ctatgctgtg agaaaactgg tcaatatgcg gattttttca
     6121 gacgcagaag gcaagatgaa cctgtctgtc aaagatattg aaggagaaat cctctctatt
     6181 tctcagttta ccctctttgc ggatactaag aaaggcaatc gtccagcctt tacaggggca
     6241 gctaaacctg atatggcatc agacttctat gatgctttca atcaaaaatt agcgcaagaa
     6301 gtgcccgttc agacaggcat ctttggagca gatatgcagg ttgagctggt taataacgga
     6361 cctgttacca ttatcctaga cactaaaaag agataagaaa gaccaagccc agtcggcttg
     6421 gtctttctca tcgatcataa aatactccaa aaagaaatcg gttcttgata tgcttggggg
     6481 aagtctcttt tcaggctttg gcagatgcga taggaaggga tgagatgtcc taggatgagg
     6541 agagttccct gaaggaaaag tggaatatca cttaaagaca ccaaggagag aatcatcagg
     6601 ctatcccata ggagaatttg taaagcaaaa cgccagaatc tcggtctaat ctgggaatag
     6661 agttgactaa agtggtcaca aagttggttt gaaagatagg tcaatagcac aggatgaaag
     6721 aaaatgagcc aaccgctata aatcaaaatc cagtcccaag taagcccccc tttttaaatg
     6781 tcaaaaatgc cagcatgatt ccatcaatga caaggagttg aatggttttg atgaagatga
     6841 tttttttcat gctaaaacct ccttttcctt agagacactc ccataaagag atgtgtttgt
     6901 cgtaaaagtc tggatagttt tctcctaggt agtcagctgt catataataa aaagtagaat
     6961 ggcaagcagt gataattggc ataagagatg aaaaacgctg agaactaatg aaggctaaga
     7021 gaacaaaaaa tagaaaatca atggtgagaa cccctaaata gtagaagcga atctttttat
     7081 tgatttgagg ataaatctca gcgaattgat gtttgagtcg gacgaccaag agaaatagca
     7141 ggagtccttg agcaaggaag aaaattaaac cagagagcaa gagccactca aaagatgtct
     7201 cgagtctagt gataaaaaat gcaaatagta gcaaatcaat agctgcaatt gctataaaat
     7261 ggattctata gagttttttc atgacaagac ctccctcttt tatctaactt cattctactc
     7321 caaaagaatg ggagttacaa ctaaaatgat aaaaatagca gaagggagat tctcttaagt
     7381 tggctagtat tctttatttg agtttccttc tattatctaa cttcttcatc attccagaca
     7441 aataaagctc cgattgcatt gaggatataa aagatgtatt taccgatatt ggcgaagttt
     7501 ccttgaatac cagcttttgt cagctgaacg aaattgtaaa tcaaccaaaa gccccactgt
     7561 ccctcattgc caatgcattc aaagcattgg caatgaggga cagtgcaaag gcaatagttg
     7621 ttacgtaggc aaggagattc atcttgcccc catatccgat atagttggtc acaaaggcaa
     7681 agaggaaggc gatgatggaa atgatgatgg ccgccaattt tacctgtttt tggctcattt
     7741 ggttgggtct gccttcttgc gaagctttcc atttctttat agcaaaggta taaatgagga
     7801 aggtgacggg ataggtaatg atggccgcct tatttccaag gatataatca atagcaccgg
     7861 acaaaatggt attaacaata ccaaagtaat ttccccattt gcttaatttc cccgtgaaac
     7921 gagtggacaa catggaaatc ccaacgttgg ttacggaaat caatccaaag ggtacaagag
     7981 ctgtccatgg tccccagtct acaaatttat cgaggcgtga gttgaggtaa ccagatgcaa
     8041 tcgcaatcca acgaccaaag caaccccgaa gaggtcaaac tatttagatg tagcaaaaat
     8101 ttttagtgat tttttcatag gttaaactac ctttcttttt ttcaaatact ctctcaccaa
     8161 atgaaagtaa aataaaatga tagaaataaa accctgaaaa taaaactttc agggttggta
     8221 ggggacttga gaaattagtt gattttctcg acatagagtt gttttgcaat gtccatattc
     8281 acttgtaaat cctcgtcgtt actgaggata gtgaaggtat tgctgaagcc atcaaactgc
     8341 ttgacttgga gctggtcacc gatgtgaagt gagtgcttgt ccagataatg gagaatgtca
     8401 aaattatcgt gcacccgagt caggcggtag gcgccagctt ccttgatatc agctagtggg
     8461 aggttattga tttcaacgag gagttctccc ttggcaggaa tagttcctcc gtgggggcag
     8521 gttttaggga aacctagcag tttatctagt ctttccacga acaggtcaga gacagtgtgt
     8581 tccaagacct cagcttcctc gtgaatctgg tcacttgtat agtctaaatg atgaactaga
     8641 aaaacttcaa tcaagcggtg cttacgatag agctcagaga ccagtttgag accgaggtca
     8701 gtcagtagat agccacattc cttgtccttt aggatgagat tttcactttt cattcgtttg
     8761 atcatttcag ttacggcagg gggagagact tgcatgcgag ccgcaatttc cttgttggta
     8821 atcttatgca ggtctatgcc aatttcataa atacatttta gatagtcttc tttgtttggg
     8881 gtcattcgtt tcctcttgtc taatatcttt atctagtata tcaaaaaaag ctagatttct
     8941 ctagctgtga ctttttatgt tatacttatt gcaagagaaa aaacgaggag atggatatga
     9001 cgaaaatagc tcttctttca gatattcatg gaaataccac cgccttggag gctgttttgg
     9061 cagatgctcg gcagctagga gtggatgaat actggctttt gggagatatt ctcatgccag
     9121 ggacagggcg tagaaggatt ttggacttgt tggatcaact accgattacg gctagagttt
     9181 tgggaaactg ggaagacagt ctttggcatg gtgtccgtaa ggaattggac agtactcgcc
     9241 ccagtcaacg ctatctcttg cgccagtgcc agtatgtttt agaggaaatt tccctagaag
     9301 aaattgaagt gctccacaat caacctcttc aaattcatcg tcagtttggg gatttgacgg
     9361 tgggaattag ccatcatctg cctgataaga actgggggcg agagttgatt catactggca
     9421 aacaagagga gtttgaccgc ttggtgactc atcctccctg tgatattgct gtttatggac
     9481 atattcacca gcagttgctt cgttacggga ctggtgggca attgattgtc aatccgggtt
     9541 cgattggcca acctttcttt ttggacgctc agctacggaa ggacttgcgg gcccagtata
     9601 tgattttgga gtttgatgac aaaggcctgg tagatatgga cttccgacgg gtagactacg
     9661 atgtggcagc ggaattgcag ctggctaaag acctgagact tccctatttt gaggtttact
     9721 atgaaagtct ggtcaatggg atccaccata ctcatcatca ggaatttttg agagaattgg
     9781 ctcagaaaga gggctgcgat cgggagttag acgactggtt gaaaagtggt aacgattgac
     9841 attacaacta aaaagggtat aataatagaa aaagaagagt agaggcaggg tagcctcgta
     9901 aaaaggagaa gaggatgcaa attccaagta gatttaccat tgcgactcat atgctgataa
     9961 tcattgccct cgaggggaag gaaagcaagg tgaccagtga ttttctggct gctagtgtcg
    10021 gggtcaatcc tgtcattatc agaaagatct tgtcccagtt gaagaaggca gagctgattt
    10081 cagtagcgcg tggaacgggc ggaacagaga ttgtcaagga ccttaaggat attagtcttt
    10141 tagatgttta tcaggcggtc gaatgtcttg gtaagacagg tcaactcttc agtttccatg
    10201 acaatccgaa tccaaattgc cctgtaggag ctcatattca tgatgttttg gatcaaaaat
    10261 tggagagaat tcagttgact atggaggcag aacttggtca aaccagtcta gaaaaagtcg
    10321 tggccgatgc agagagtcag atgaaggatt aagagggata aatgccctct tttttctttg
    10381 gataattatg ataaaatgta gctattaaag gagtaagaaa tgtatctcgt tatagaaatt
    10441 gtattagcct ttatagtgtc attttggaag gataatagaa gtttgtggaa tccagtatta
    10501 tttttattat ccctcatttc aagttatttt tatctatcct acctttttta taaaaatgga
    10561 tacgagaatg ttcagttagc tttttatgta tttgcctttg tcttacttcc ttttttgctt
    10621 tttctaagtg gcatcttttt aatctataat ggggtcattt tgttgaaacg agagggacgt
    10681 tccaagcccc attatctctc aatgctcttt gatttttatt gactattaga ttctatttaa
    10741 aaagacttag aatgcacatg aaagtatgta ttctaggtct tttgttttgt atacatttaa
    10801 ttttcaagag tattatctat ttttccatgc atggtaacga gtatcaatca ataactgggt
    10861 aaggcgaccc aaggtttctc tttcttctgg agtgagggat tgagcttgga caaagagatg
    10921 atgaatgtat tcaagcgcct ttaaggaagc caagtcattg atagagctaa taccagcatc
    10981 aagaatagtg ccaaagaaga ggtcgaagta gagctgaagt tcttcgtcag ggactgtggc
    11041 cacccattct ttaaaggttg tgtctacttg ccggctatca ctgttggtct tatccagttg
    11101 aacgaagtgc ttgtcctcaa tctgccaact aaaggtatcg tgctgggcga tgccacccag
    11161 ggcagtactc tgaacgatga tttggtgagc aggaatttcc agcatcatac cgataatgga
    11221 accttgtgga atgaatatct tgcttctatc cattatcctt tgataacccg cagtctgtgt
    11281 caattcttga tggagaccag gtgcatcaaa tgtataaact gctgtgatct gattttgcaa
    11341 actttgctca atttggctag cagcatagat agcgagattt cctcccttgg aatgcccagc
    11401 cagaataacc ttttgcttag gatgatgggc aaaaaagttc tttaaatagc gaagggcgtg
    11461 cttttgagca ggaatttcct tcatataggt caggtggaaa tcttccttcc agccaatgat
    11521 actgtcatct gtcccacgaa agacaatcag ataggtatcg aggctgacac gataagtcat
    11581 agccgcaaat tgcttttgca gttcagggtc gatgtcgttg ataaaatggg agagtttgca
    11641 atttttgaag cgcttgtgtt gagccaattc atctaataat tgaaggcgat ttttgctagt
    11701 aagcatggtg ggctctcttg gaacctgagg tgctagatct aaaagacgtt gaggaagtgt
    11761 ggagaccaga ttatcaaagg agaggtaggt gatttctatt aaggttagaa tgtctaactc
    11821 attcaaggga aggtcgtaat aagaatcata tgcgacatct ttcagatagt caaaaatatt
    11881 ggccataaga gctcctttct ttttatttat cttagcaaat ttccctcaaa ttagctagta
    11941 gcattgcctg tttgtactgg ctaaaaacag gctatttcaa attcagtttc agaccatctg
    12001 gcatggaaaa atctgttata ataatagaaa aggagaagcg catgcacaag attttattaa
    12061 tagaagatga tcaggtcatt cgtcaacagg tcgggaaaat gctctctgaa tggggatttg
    12121 aagtggtcct ggtagaagac tttatggaag ttttgagtct atttgttcag tcggaacctc
    12181 atctggtcct catggatatt ggtttgccct tgtttaatgg ttatcactgg tgtcaggaaa
    12241 tccgcaagat ttccaaggta cctatcatgt ttctttcttc gagagaccag gctatggata
    12301 ttgtcatggc aatcaatatg ggggcggatg actttgtgac caagcctttt gaccagcagg
    12361 ttcttttagc taaggttcag ggcttgttgc gtcgttccta tgagtttggg cgtgatgaga
    12421 gtttgctgga atatgctggt gttatcctca ataccaaatc catggattta cattatcaag
    12481 ggcaagtctt gaatttgacc aagaatgaat tccagatttt acgcgtgtta tttgagcatg
    12541 caggcaacat cgtagcacgt gacgacctga tgcgggaact ttggaacagt gactttttca
    12601 ttgatgataa taccctctct gtcaatgtgg ctcgtttgcg taaaaagttg gaggagcagg
    12661 gattggtagg atttatcgag accaagaaag gaatagggta cggattgaag catgcttgat
    12721 tggaaacaat tttttctagc ctatctgcgc tcccgtagtc gtctttttat ctatctgctt
    12781 tctttggcat ttcttgtctt actctttcag tttttatttg ccagtctagg aatttacttc
    12841 ctctactttt tcttcttgtg ttgctttgta accatcttat ttttcacttg ggacatattg
    12901 gtggaaacgc aggtctatcg ccaggaactt ctctatggag agagggaagc caagtctcct
    12961 ttggaaatag ctttagcaga aaaattagaa gcgcgtgaga tggaactcta tcagcagagg
    13021 tcaaaagcag aaagaaaact gacggatttg ctggattact ataccttgtg ggtccatcag
    13081 ataaagaccc ccattgcagc cagtcaactc ttagttgcag aagtggtcga ccgccaactg
    13141 aagcagcagc tagaacagga aattttcaaa atcgactcct ataccaacct agttttacag
    13201 tacctgcgtt tagaaagttt ccatgatgat ttgctcttaa agcaggttca aattgaggac
    13261 ttggtcaagg aaataattcg taaatatgct cttttcttta ttcaaaaagg cttaaatgtc
    13321 aatctacatg accttgataa agaaatcgtg acggataaaa agtggctgct agtggttatt
    13381 gagcaaatca tctcaaacag tctcaagtac accaaggaag gtggtctgga gatttatatg
    13441 gatgaccaag agctttgtat caaagatacg ggaatcggga taaaaaacag tgatgtcctc
    13501 cgagtatttg aacgtggctt ttcaggatac aatggccgtt tgacccagca gtcctctgga
    13561 cttggccttt atctatctaa gaaaatttct gaagaactgg ggcaccagat tcgtatcgag
    13621 tctgaggtcg gaaaaggaac gacagtgcgg attcagtttg ctcaagtgaa cttagtcctt
    13681 gagtaaaaat agatgagaaa aagcctccag attggaggct tttatgctag actcttattt
    13741 ctcaacgcgt gatttgttgg cgatatcagc taggatagct tgaacaaaat tatcaactga
    13801 gacagtttgt gtttcttttt ggccgtagcg acgaacgttg actgtttcgt cttccatttc
    13861 tttgtctcca acaattaatt ggtaaggaat cttgctggtt tgtgaagcac ggatcttgaa
    13921 ctgcattttt tcattgcgct catctacgtc tgcacggaca ccgcggtcac ggagtttctt
    13981 ggccacttcc caagcgtagt ccacgtgttt ttcgttagat actgggatga gggttacttg
    14041 gtgtggtgcc agccatgttg ggaaggcccc cttgtagttc tcaatcaaga tagctgtgaa
    14101 gcgttccata gttgagataa ccccacggtg gatcatgact ggacggtgat cttcgccatc
    14161 agctccgatg tatttgaggt cgaagcgttc tggcaacaag aaatcaagtt ggatagtaga
    14221 aagggtttct tcttttccaa gggcagtttt aatctggata tccaattttg gtccgtagaa
    14281 ggctgcttca ccttcggctt caaagtagtc cacgcccatt tcatcaagag ctgcacgaag
    14341 catggtttgg gcattttccc acatctcatc gttatcaaag tacttatgag tatcttgagg
    14401 gtcacgaaga gagaggcgga agcggtagtc agtcaagttg aagtcttcat aaacatcgat
    14461 aatcaactga agggcacgtt ggaattcttc ttggatttgt tctggagtaa cgaataggtg
    14521 accgtcgttg agtgacattt cacgtacacg ttgaaggcca gtgagggcac cagatttttc
    14581 gtaacggtgc atcataccga tttcagcgat acggattggc aattcacggt aagagtgaac
    14641 atggtgtttg aaaacttgga tgtggtgcgg acagttcatt ggacgaagga caaattcttc
    14701 cccgtccccc atgtccatgg ttgggaacat gtcttcttgg taatgatccc agtgaccaga
    14761 agtcttgtaa agctcaacag aagcaagtgg tggagtgtag acgtgttggt agccagaagc
    14821 caactctttg tttacgatgt agcgttccaa ttcacgacgg atagtcgcac catttggcaa
    14881 ccagaatggc aaaccttgtc ccacttcttg tgaaatcata aagaggtcaa gctctttacc
    14941 aagtttacgg tggtcacgtt ccttagcttc ttcacgcatt tgaaggtagt ttttcaagtc
    15001 tttcttgtca aaccaagctg taccgtagat acgttgcatc atagcgttgt cgctgtttcc
    15061 acgccagtac gcaccagcta catggagaag gtggaagatt tggatacgac ctgttgatgg
    15121 aacgtgaggt ccacggcaga ggtctacata ttcaccctga cgatagatag tcaaaccgcc
    15181 ttcgtcttct gagtgttctt caatcaattc caacttgtaa gggtcatttt tgaagatttt
    15241 acgtgcctcg tctttagtca cttcttcacg aatagatggg aagttttctt tgacgatttt
    15301 ttgcatttct tcttcgatac gaggaaggtc ttcgttagag atttgaccag ctgtgttgtc
    15361 agtatcgtag tagaaaccat cttcgatggc tggaccaact cccaagtgaa tgtctgggaa
    15421 aagacgacga gctgcttggg cgaacaagtg agctgctgag tgacgcaaga ttggaagggc
    15481 atcttcgtga tcaggtgtca caatttcgat gcttccatct tcagtgatag cgcgagtagt
    15541 gtcgatgagt ttgccgttga atttaccagc caaggctttt ttagctaggg aattgctgat
    15601 agattgggca atttcaaaag ttgttacgcc agattcgaat tcacgaacag cgccatctgg
    15661 gaaagtaatg ttaatcatga tgttctcctt ttttattatt tcatagtttc taagcattga
    15721 ttttttatca tcagactact tccaagtcct aacgttcaat tgcttttctt tatcagagag
    15781 gaagttttcc atttccgtca gaatgtttat atccaaacgt tgagaaagtt ctaatagcca
    15841 ccagatattt tctgaaagtt tttattccag tgtgtagggt gtttcatcat agtagcgtcc
    15901 ttgctttgtc atcaccagtc gttggaaatt tccaatatca ttagataaag ccaagaggtc
    15961 ttcttctacc gtccacttgg aatcatgatg cttaacttcc agttcgtgat aagcttgtcg
    16021 gattgcccaa ctgcgctcca ctaattcttg caactccata attagtatca taataaaaaa
    16081 atgcgacatc ctcgaaagga cgtcgcaacg tggttccacc ttcatttatg tacctcaaaa
    16141 gagagggaca cctctgattg gctctaacgt ggccaccgtt ttatgttttc ataaaaaact
    16201 caagagtagt atcagtttag acttcctatg cgtttccagc aaccacgcac tctctagtag
    16261 aaagggacta agtgacttgt ctctatggat tattatagca tgattgtgag atttttcaag
    16321 gattttgtga aagttttttg tttttcattt tttgtagaat atgcttgatg ctaataccag
    16381 aatccatgcc caccagagca actgtttcat agattagcaa gtaaattagt ataaactcac
    16441 caaaggttat gatagtaaag taaaagagca gtccgttacc aactaaccat caaagtgaga
    16501 aacggaaacg gtagctatag agtaacgata caatcaagat taccagaggt gtaaagaaga
    16561 gaagattgtt tatatagagt cccttgagtg taagggggtc agaaacaact agtaagaaca
    16621 actcataaag tggccaataa aagaataata tgaccagata gtaggggaga tgggaataga
    16681 atctacgcat gataatcacc tcgtttaaaa aaaacagaca acttagatct cttgctatga
    16741 ttattatagc atgtttaaaa caagaaagta aatttgttga caaagaagat ttttcttttc
    16801 actagaatca tggttagttt tcttggtaga aatattttga taataattaa gcaagactat
    16861 tgacattgat tattaagagt gtcacaattg ttgtaactaa aataattaca acaagagagg
    16921 tataaaaaat gaaactagca gttattgcag caaatggaca agtggggaag gcaattgttg
    16981 aagaagcagt caaacgagga catgaagtaa cggctattgt tcgttctgaa aacaaaagtc
    17041 aagctgagtc tattattaaa aaagatttat ttgagttgac aaaagatgat cttactggat
    17101 ttgatgctgt tatttcagcg tttggtgctt atacaccaga tacgctaccg ctccatagca
    17161 aatcaatcga actatttaat cagcttcttg ctggaacaca gacacgtttt ctagttgtag
    17221 ggggagcagg aagtctttat atagatgaaa caaaaacgac acgactttta gatacgcctg
    17281 attttcctga agaatttaag tcactagcaa aagctcaagc ggatgaattg gatcttcttc
    17341 gtacaaaaaa taatctcaac tggacttttg tcagtcctgc tgtagatttt attcccgatg
    17401 gtgaaaaaac aggaaactac attctagcag gagaaatttt tactactaat gaaaaaggaa
    17461 taagccagat tagttatgca gattatgcta ttggattggt agatgaactt gaaaaaggtc
    17521 atcatataaa agaacgtatc tctttacttg aaaaataata actatgagag tattaatgtt
    17581 tgacagaaaa tttaaatctt ggtaaaatag ggattgtcgt aaagacaaat aacttcttct
    17641 tggttacagg catgccaacc tgtcactcgg atgaagccaa ataaaaagga gaaacatcat
    17701 ggcaatctca aaagagaaaa aaaatgaaat cattgcacaa tatgcacgtc acgaaggtga
    17761 tacaggttca gtagaggttc aagttgctgt ccttacttgg gaaatcaacc accttaacga
    17821 acacatcaaa caacacaaaa aagaccatgc tacttaccgt ggattgatga aaaaaatcgg
    17881 tcgccgtcgt aacttgcttg catacttgcg taaaaacgac gttaaccgtt accgtgagtt
    17941 gatcaactct ctaggacttc gtcgctaatt caagatacaa gtgcttaggc cgtgttcaat
    18001 tgagcatatc ttgataatga agctactcta aattgggtag cttttttgcg ttgtcacctt
    18061 acctcgctat actcaagtat aatctttggt tacggttcct agcactgtaa ggtaaaataa
    18121 accagctttt
//
LOCUS       DAVMHG010000031        18026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMHG010000031 DAVMHG010000000
VERSION     DAVMHG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.031"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..192
                     /locus_tag="VYR08_000829"
     CDS             <1..192
                     /locus_tag="VYR08_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV3466390.1"
                     /translation="MVKDVNEDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPA
                     MSAPVFDNIEAAIKEHGLS"
     gene            240..1715
                     /locus_tag="VYR08_000830"
     CDS             240..1715
                     /locus_tag="VYR08_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV3466391.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2020..8721
                     /locus_tag="VYR08_000831"
     CDS             2020..8721
                     /locus_tag="VYR08_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV3466392.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSERSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVHYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKIVAAVQENLPKLEFVENDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8969..9821)
                     /locus_tag="VYR08_000832"
                     /pseudo
     CDS             complement(8969..9821)
                     /locus_tag="VYR08_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10033..10392)
                     /locus_tag="VYR08_000833"
     CDS             complement(10033..10392)
                     /locus_tag="VYR08_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV3466393.1"
                     /translation="MGMILMKLASILLLILTLVICIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            10484..11440
                     /locus_tag="VYR08_000834"
                     /pseudo
     CDS             10484..11440
                     /locus_tag="VYR08_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11424..11981
                     /locus_tag="VYR08_000835"
     CDS             11424..11981
                     /locus_tag="VYR08_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3466394.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPAADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11974..12228
                     /locus_tag="VYR08_000836"
     CDS             11974..12228
                     /locus_tag="VYR08_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466395.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAMGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12240..14294
                     /locus_tag="VYR08_000837"
     CDS             12240..14294
                     /locus_tag="VYR08_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV3466396.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14473..15333
                     /locus_tag="VYR08_000838"
     CDS             14473..15333
                     /locus_tag="VYR08_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV3466397.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQTLQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGVLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15544..16254
                     /gene="ccdA2"
                     /locus_tag="VYR08_000839"
     CDS             15544..16254
                     /gene="ccdA2"
                     /locus_tag="VYR08_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3466398.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIIFLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16274..16840
                     /locus_tag="VYR08_000840"
     CDS             16274..16840
                     /locus_tag="VYR08_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV3466399.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16851..17963
                     /gene="msrB"
                     /locus_tag="VYR08_000841"
     CDS             16851..17963
                     /gene="msrB"
                     /locus_tag="VYR08_000841"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV3466400.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         5426 a         3418 c         3970 g         5212 t
ORIGIN      
        1 atggtgaaag atgtaaacga agaccttatg aaaggttacg atatctttac accaatcgct
       61 gcaacagacc ttggttttga accaggtatc ccagttatcg aagctgggcc aatcttattc
      121 cgtatcccag caatgagcgc tccagtattt gacaatattg aagcagctat taaagaacat
      181 ggattaagct aattctaatt tgttaacatt catataacat aaaaaaggga ggaactgtta
      241 tggatgcaat ctttgaccta atcggaaagg ttttcaatcc catcttagaa atgggtggac
      301 ctgtcatcat gttgatcatt ttgacagtat tggctttact ttttggagtg aaattctcca
      361 aagcgcttga aggtggtatc aaacttgcca tcgctcttac aggtatcggt gctatcatcg
      421 gtatgctaaa tggtgctttc tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc
      481 aattgagtat tacagacgtt ggttgggcac cactcgcaac aatcacttgg ggttctgctt
      541 ggacactata cttcttgctc atcatgttga ttgtcaacgt agtgatgctt gctatgaaga
      601 aaaccgatac acttgatgtc gatatctttg atatctggca cttgtctatc acaggtctct
      661 tgattaaatg gtatgctgat aacaatggtg tgagtcaagg ggtttcactc tttattgcta
      721 cagcagctat cgtccttgtc ggtgtgttga aaattatcaa ctctgacttg atgaaaccta
      781 catttgatga ccttcttaac gccccaagtt catcaccaat gacatcaact cacatgaact
      841 acatgatgaa cccagttatc atggttttgg ataagatttt tgaaaaattc ttcccaggcc
      901 ttgataaata tgactttgat gctgctaaat tgaacaagaa aatcggtttc tggggatcta
      961 aattcttcat cggtttcatc cttggtatcg ttatcggtat tatgggaact ccacatccaa
     1021 ttgcaggtgt tgcagatgca gataaatggc gtcttgttat caaaggatgg ttgtctcttg
     1081 gtttgactgc cggtgtatct ttggaactct tctcacttat cggttcatgg ttcatcgcag
     1141 ccgtagaacc actatcacaa ggtattacaa acgttgctac taaacgtctt cgaggacgta
     1201 aattcaatat cggtcttgac tggccattca tcgctggtcg tgctgaaatc tgggcttgtg
     1261 ccaacgtact tgcaccaatc atgttgattg aagcagtgct tctttcaaaa gttggaaatg
     1321 gtatcttgcc acttgcaggt atcatcgcta tgggtgttac tccagctctc ttggttgtaa
     1381 ctcgtggtaa attgctccgt atgattatct tcggaacact cttgttgcca ctcttccttc
     1441 tttcaggtac acttattgca ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc
     1501 cagaaggtgt gagccaaact caattgatta ctcactctac tcttgaagga ccaatcgaaa
     1561 aacttcttgg ttggacaatt ggtaacacta caactggtga tatcaaagca atccttggtg
     1621 cagtagtctt ccttgtattc tatatcggta tctttgcttg gtacagaaaa caaatgatca
     1681 aacgtaacga agagtacgca gcaaaagcaa aataatgcgc tcctataaaa tataaactca
     1741 atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctaaaaaca cagttttgag
     1801 gttgtagata agactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact
     1861 gacgaagtca gtaatcatat ctacggcaag gcgacgctga cgaagtttga attggatttt
     1921 cgcagagtgt aaattgttga aactaggttg tcataccatg tataccactt gggctttggt
     1981 agtagctaac tgcactaaat ataatataag gagggtttta tggggaaagg ccattggaat
     2041 cggaaaagag tttatagcat tcgtaagttt gctgtgggag cttgctcagt aatgattggg
     2101 acttgtgcag ttctattagg aggaaatata gctggagaat ctgtagttta tgcggatgaa
     2161 acacttatta ctcatactgc tgagaaacct aaagaggaaa aaatgatagt agaagaaaag
     2221 gctgataaag ctttggaaac taaaaatgta gttgaaagga cagaacaaag tgaacctagt
     2281 tcaactgagg ctattgcatc tgagaagaaa gaagatgaag ccgtaactcc aaaagaggaa
     2341 aaagtgtctg ctaaaccgga agaaaaagct ccaaggatag aatcacaagc ttcaagtcaa
     2401 gaaaaaccgc tcaaggaaga tgctaaagct gtaacaaatg aagaaatgaa tcaaatgatt
     2461 gaagacagga aagtggattt taatcaaaat tggcacttta aactcaatgc aaattctaag
     2521 gaagccatta aacctgatgc agacgtatct acgtggaaaa aattagattt accgtatgac
     2581 tggagtatct ttaacaattt cgatcatgaa tctcctgcac aaaatgaagg tggacaactc
     2641 aacggtgggg aagcttggta tcgcaagact ttcaaactag atgaaaaaga cctcaagaaa
     2701 aatgttcgcc ttacttttga tggcgtctac atggattctc aagtttatgt caatggtcag
     2761 ttagtggggc attatccaaa tggttataac cagttctcat atgatatcac caaatacctt
     2821 cacaaagatg gtcgtgagaa tgtgattgct gtccatgcag tcaacaaaca gccaagtagc
     2881 cgttggtatt caggaagtgg tatctatcgt gatgtgactt tacaagtgac agataaggtg
     2941 catgttgaga aaaatgggac aactatttta acaccaaaac ttgaagaaca acaacatggc
     3001 aaggttgaaa ctcatgtgac cagcaaaatc gtcaatacgg acgacaaaga ccatgaactt
     3061 gtagccgaat atcaaatcgt tgaacgaggt ggtcatgctg taacaggctt agttcgtaca
     3121 gcgagtcgta ccttaaaagc acatgaatca acaagcctag atgcgatttt agaagttgaa
     3181 agaccaaaac tctggaccgt tttaaatgac aaacctgcct tgtacgaatt gattacgcgt
     3241 gtttaccgtg acggtcaatt ggttgatgct aagaaggatt tgtttggtta ccgttactat
     3301 cactggactc caaatgaagg tttctctttg aatggtgaac gtattaaatt ccatggagta
     3361 tccttgcacc acgaccatgg ggcgcttgga gcagaagaaa actataaagc agaatatcgc
     3421 cgtctcaaac aaatgaagga gatgggagtt aactctatcc gtacaaccca caaccctgct
     3481 agtgagcaaa ccttgcaaat cgcagcagaa ctaggtttac tcgttcagga agaggccttt
     3541 gatacgtggt atggtggcaa gaaaccttat gactatggac gtttctttga aaaagatgcc
     3601 actcacccag aagctcgaaa aggtgaaaaa tggtctgatt ttgacctacg taccatggtc
     3661 gaaagaggca aaaacaaccc tgctatcttc atgtggtcaa ttggtaatga aataggtgaa
     3721 gctaatggtg atgcccactc tttagcaact gttaaacgtt tggtcaaggt tatcaaggat
     3781 gttgataaga ctcgctatgt taccatggga gcagataaat tccgtttcgg caatggtagc
     3841 ggagggcatg agaaaattgc tgatgaactc gatgctgttg gatttaacta ttctgaagat
     3901 aattacaaag cccttagagc taagcatcca aaatggttga tttacggttc agaaacatca
     3961 tcagcaaccc gtacacgagg aagttactat cgccctgaac gtgaattgaa acatagcaat
     4021 ggacctgagc gtaattatga acagtcagat tatggaaatg atcgtgtggg ttgggggaaa
     4081 acagcaaccg cttcatggac ttttgaccgt gacaacgctg gctatgctgg acagtttatc
     4141 tggacaggta cggactatat tggtgaacct acaccatggc acaaccaaaa tcaaactcct
     4201 gttaagagct cttactttgg tatcgtagat acagccggca ttccaaaaca tgacttctat
     4261 ctctaccaaa gccaatgggt ttctgttaag aagaaaccga tggtacacct tcttcctcac
     4321 tggaactggg aaaacaaaga attagcatcc aaagtagctg actcagaagg taagattcca
     4381 gttcgtgctt attcgaatgc ttctagtgta gaattgttct tgaatggaaa atctcttggt
     4441 cttaagactt tcaataaaaa acaaaccagc gatgggcgga cttaccaaga aggtgcaaat
     4501 gctaatgaac tttatcttga atggaaagtt gcctatcaac caggtacctt ggaagcaatt
     4561 gctcgtgatg aatctggcaa ggaaattgct cgagataaga ttacgactgc tggtaagcca
     4621 gcggcagttc gtcttattaa ggaagaccat gcgattgcag cagatggaaa agacttgact
     4681 tacatctact atgaaattgt tgacagccag gggaatgtgg ttccaactgc taataatctg
     4741 gttcgcttcc aattgcatgg ccaaggtcaa ctggtcggtg tagataacgg agaacaagcc
     4801 agccgtgaac gctataaggc gcaagcagat ggttcttgga ttcgtaaagc atttaatggt
     4861 aaaggtgttg ccattgtcaa atcaactgaa caagcaggga aattcaccct tactgctcac
     4921 tctgatctct tgaaatctag tcaagtcact gtctttactg gtaagaaaga aggacaagaa
     4981 aagactgttt tggggacaga ggtgccaaaa gtacagacca ttattggaga ggcacctgaa
     5041 atgcctacca ctgttccgtt tctatacagt gatggtagtc gtgcagaacg tcctgtaacc
     5101 tggtcttcag tagatgtgag caagcctggt attgtaacgg tgaaaggtat ggctgacgga
     5161 cgagaagtag aagctcgtgt agaagtgatt gctcttaaat cagagctacc agttgtgaaa
     5221 cgtattgctc caaatactga cttgaattct gtagacaaat ctgtttccta tgttttgact
     5281 gatggaagtg tacaagagta cgaagtagat agctgggaga ttacggaagt agataaagcc
     5341 aaactttcag tagctggatc acgtattcaa atgactggtc agttagctgg agaaactatt
     5401 catgcaaccc ttgtggtaga agaaggaaat gctgcagcac ctgtagtgcc aactgttact
     5461 gttggaggtg aagcagtaac aggtcttact agtcgacaac caatgcaata tcgtactcta
     5521 tcttatggtg cccaattgcc agaagtcaca gcaagtgctg aaaatgctga tgtgacagtt
     5581 cttcaagcaa gcgcagcaaa cggcatgcgt gcgagcatct ttattcagcc taaagatggt
     5641 ggccctcttc aaacctatgc aattcaattc cttgaagaag cgccaaaaat tgctcacttg
     5701 agtttgcaag tggaaaaagc tgacagtctc aaagaagacc aaactgtcaa attgtcggtt
     5761 cgagctcact atcaagatgg aacgcaagct gtattaccag ctgataaagt aaccttctct
     5821 acaagtggtg aaggggaagt cgcaattcgt aaaggaatgc ttgagttgca taagccagga
     5881 gcagtcactc tgaacgctga atatgaggga gctaaaggcc aagttgaact cactatccaa
     5941 gccaatactg agaagaagat tgcgcaatcc atccgtcctg taaatgtagt gacagatttg
     6001 catcaggaac caagtcttcc agcaacagta acagttgagt atgacaaagg tttccctaaa
     6061 actcataaag tcacttggca agctattccg aaagaaaaac tagactccta tcaaacattt
     6121 gaagtactag gtaaagttga aggaattgac cttgaagcgc gtgcaaaagt ctctgtagaa
     6181 ggtatcgttt cagttgaaga agtcagtgtg acaactccaa tcgcagaagc accacaatta
     6241 ccagaaagtg ttcggacata tgattcaaat ggtcacgttt catcagctaa ggttgcatgg
     6301 gatgcgattc gtccagagca atacgctaag gaaggtgtct ttacagttaa tggtcgctta
     6361 gaaggtactc aattaacaac taaacttcat gttcgcgtat ctgctcaaac tgagcaaggt
     6421 gcaaacattt ctgaccaatg gaccggttca gaattgccac ttgcctttgc ttcagactca
     6481 aatccaagcg acccagtttc aaatgttaat gacaagctca tttcctacaa taaccaacca
     6541 gccaatcgtt ggacaaactg gaatcgtagt aatccagaag cttcagtcgg tgttctgttt
     6601 ggagattcag gtatcttgag cgaacgctcc gttgataatc taagtgtcgg attccacgaa
     6661 gaccatggag ttggtgcacc gaagtcttat gtgattgagt attatgttgg taagactgtc
     6721 ccaacagctc ctaaaaaccc tagttttgtt ggtaatgagg accatgtctt taatgattct
     6781 gccaactgga aaccagttac taatctaaaa gcccctgctc aactcaaggc tggagaaatg
     6841 aaccacttta gctttgataa agttgaaacc tatgctattc gtattcgcat ggttaaagca
     6901 gataacaagc gtggaacgtc tatcacagag gtacaaatct ttgcgaaaca agttgcggca
     6961 gccaaacaag gacaaacaag aatccaagtt gacggcaaag acttagcaaa cttcaaccct
     7021 gatttgacag actactacct tgagtctgta gatggaaaag ttccggcagt cacagcaagt
     7081 gttagcaaca atggtctcgc taccgtcgtt ccaagcgttc gtgaaggtga gccagttcgt
     7141 gtcatcgcga aagctgaaaa tggcgacatc ttaggagaat accgtctgca cttcactaag
     7201 gataagaact tactttctca taaaccagtt gctgcggtta aacaagctcg cttgctacaa
     7261 gtaggtcaag cacttgaatt gccgactaag gttccagttt acttcacagg taaagacggc
     7321 tacgaaacaa aagacctgac agttgaatgg gaagaagttc cagcggaaaa tctgacaaaa
     7381 gcaggtcaat ttactgttcg aggccgtgtc cttggtagta accttgttgc tgaggtcact
     7441 gtacgagtga cagacaaact tggtgagact ctttcagata accctaacta tgatgaaaac
     7501 agtaaccagg cctttgcttc agcaaccaat gatattgaca aaaactctca tgaccgcgtt
     7561 gactatctca atgacggaga tcattcagaa aatcgtcgtt ggacaaactg gtcaccaaca
     7621 ccatcttcta atccagaagt atcagcgggt gtgatcttcc gtgaaaatgg taagattgta
     7681 gaacggactg ttgcacaagg aaaagttcag ttctttgcag atagtggtac ggatgcacca
     7741 tctaaactcg ttttagaacg ctatgtcggt ccagagtttg aagtgccaac ctactattca
     7801 aactaccaag cctacgacgc agaccatcca ttcaacaatc cagaaaattg ggaagctgtg
     7861 cattatcgtg cggataaaga cattgaagcc ggtgatgaaa ttaacgttac ctttaaagct
     7921 gtcaaagcca aagccatgag atggcgtatg gagcgtaaag ctgacaagag cggtgttgcg
     7981 atgattgaga tgaccttcct ggcaccgagt gaattgcctc aagaaagtac gcaatcgaag
     8041 attcttgtag atggaaaaga gcttgctgat ttcgctgaaa atcgtcaaga ctatcaaatt
     8101 acctataaag gtcaacggcc aaaagtctca gttgaagaaa ataatcaagt agcttcaact
     8161 gtggtagata gtggagaaga tagccttcca gtacttgttc gcctcgtttc agaaagtgga
     8221 aaacaagtca aggaataccg tatccacttg actaaggaaa aaccagtttc tgagaagata
     8281 gttgctgctg tacaagaaaa tcttccaaaa ctcgaatttg ttgaaaatga tttggcctac
     8341 aagacagttg agaaaaaaga ttcaacactg tatctaggtg aaactcgtgt agaacaagaa
     8401 ggaaaagttg gaaaagaacg tatctttaca gcgattaatc ctgatggaag taaggaagaa
     8461 aaactccgtg aagtggtaga agttccgaca gaccgcatcg tcttggttgg aaccaaacca
     8521 gtagctcaag aagctaaaaa accacaagtg tccgaaaaag cagatacaaa accaattgat
     8581 tcaagtgaag ctagtcaaac taataaagcc cagttaccaa atacaggtag tgcggcaagc
     8641 caagcagcag tagcagcagg tttagctctt ctaggtttga gtgcaggatt agtagttact
     8701 aaaggtaaaa aagaagacta gaaattttga taccttcttt atcattaaag accaaagaaa
     8761 aactcttgtc ctaaatagaa gataaagagt ctgggacaaa atagttctca accacaaaaa
     8821 agctagatat ttccaattgc ggaactctag ctttttaatt ttgagtcgtt ctcttattgt
     8881 atagtagatt gaaactagag tagtacactt ctacttctaa aacattgtta gaaatcgatt
     8941 tgactgtcct tatcgatttg tcctgttctt atttcatttc actatgtttt aggaggttat
     9001 tggccataag taccaatccc atgtcaattc tcacttgacg cttgcctctc agattacatc
     9061 tcttgtaacc caaacaagcc tttatctgcc caaagacagg ttccacatca atcttgcgtt
     9121 gagcgaaaat ctgtctatct tggggagata aaagcgcctg acattcttta gctttcaagt
     9181 tttgatagcg ttcgttcata tacagtccct tttgaggggc tgattcaggt tcatcagcgt
     9241 agtaaacctt gatttcctgt tgaaagtctg tctgtgttgg tacttgatat ggtgaaaacg
     9301 atagcatcag ccatcaggat gtatgtagct atcctccttg tcattatagt gccaattcgc
     9361 taagttttta gctgactctt tataccctct cttttgtttc ttatcaaaca tggcatattt
     9421 aatcagaagg ggagtgattt cttcttgaaa atagacttgt atctgttctt gaagtttggc
     9481 ggagaatttc tctgtcgttt tcttccacac aaaactatac ttgttggcat tggcttcaat
     9541 cttagttccg tcaataaaca aacaatctaa ggtcactaac tcttccattt ttcaattttt
     9601 cgaccagaga aaatcccttg tgaataggca aatagaagag tagatacaag cattttaggg
     9661 tgataagacg ggcgatcaaa ggcatgatag gaggtgtaga agtgacgttc ctccaaggta
     9721 ttcaccactt tttcaatagt aaagacgaga tggtcttgtg gtaagaagga actgatttct
     9781 agtggtaaag ttgtttgatt tgtgttatag tgaatatgca tgggatagcc tttttaaatg
     9841 gtttattaag caattctatt ttaccaagac tatcgtaacc atgcaaaaaa agcaacggca
     9901 aatccgttgc tttttttgga tataagagac tattgtccca gactcttttc tttgtataaa
     9961 attgtatagt gttattgtct atactatttt gtagagaaag caaaaatccc tagaaaatgc
    10021 ttagggattc gactatttca ttaagaacaa ttcaacaatc atttctatat acatgataag
    10081 ggttaataag aatcctgcac gccagaagaa tttgatgaat ttaggatagt aaaagctgcg
    10141 ttttttcaaa aggaagaaaa agacgagaat aatggctagg aacgagaggg ctagccccag
    10201 tctagggagg aaattatggg taaaggtttt agctgtaatc aagtaatact caaataccaa
    10261 gactggaaaa gccaaatccg caaagtttcg tcctagtttt tttaatctaa aaagtttggt
    10321 taggataatg cagatgacta aggtcagtat caataataaa atagatgcta atttcattaa
    10381 aatcataccc atattgtatc ataaaaaaag gctaatggaa acgagaaaca gaggaattta
    10441 taggaaagtc aggcttttga aattgtgggt gatttttgtt ataatgaaag ttataaaatc
    10501 ttataatacc ttgaatgatt attatcgaaa actctttgga gaaaagactt ttaaagtccc
    10561 tattgatgcg ggatttgact gtcccaatcg tgatggaact gtggctcatg gaggctgtac
    10621 tttttgtacg gtttccggtt ctggagatgc cattgtggca ccagatgcgc ctatccgtga
    10681 gcaattttat aaggaaattg actttattca tcgtaagtgg ccagatgttc agaagtatct
    10741 ggtttatttt caaaatttta ccaacaccca tgaaaaggtg gaagttattc gagagcgtta
    10801 tgaacaggct atcaatgagc caggtgtggt aggaatcaat attggaacgc gcccagactg
    10861 tttaccagac gaaaccatcg aatatttggc tgagttatcg gagtgcatgc atgtgacggt
    10921 tgaattgggc ttgcaaacta cttatgaagc aacctctgac ctgattaacc gtgcgcattc
    10981 ttatgaattg taggtggaaa cggtcaagcg tttgagaaag tatccaaaaa ttgagattgt
    11041 ttcccatttg atcaatggtt tgcctggtga aacccatgag atgatggttg aaaatgtccg
    11101 ccgttgtgtc acggataacg atattcaagg gattaaactg catttgctcc atcttatgac
    11161 aaatactcgt atgcaacgag attaccatga ggggcgttta caattgatga gtcaggacga
    11221 atatgtcagg gtcatctgtg accaactgga aattattccc aaacatatcg tcatccatcg
    11281 aattacagga gatgcgccta gggatatgct gcttggtcct atgtggagcc tcaataaatg
    11341 ggaagtcctc aacagcattg agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa
    11401 ggctgtaaaa caggagtttg aaaatgaaaa gaccacttga gatggcacat gattttttgg
    11461 ctgaggtcgt gacaaaagag gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca
    11521 cgcttttttt agccaagcta gccaagcaag tctatgcctt tgatattcag aagcaagcct
    11581 tggaaaagac ccaagagcgt ttgcatcagg ctgacttgac aaatgcccag ttaatcttgc
    11641 aaggccatga gacactggac cagtttgtga taaaagctaa ggcagggatt tttaatctgg
    11701 gctatttgcc ggcagctgat aagtctgtca tcacccgacc gcagacaacg attgaggcat
    11761 tagaaaagct atgtggctta cttgtcaaag gtggacgaat tgctattatg atttactatg
    11821 gtcatgaagg aggcgatctc gagagagatg ctgtcttgga ttttgtgatc cagttgaacc
    11881 aacaagagta cacagctgcc atttaccgaa ctttaaacca agtcaacaac ccgccgtttt
    11941 tagtgatgat tgaaaaatta gagagataca gacatggata aacaatactt acacgaaaag
    12001 ctggatgcca tgcgccagaa ttttgttgaa tcaacccacc acgaacgagc gatgggcgtg
    12061 ctagaccaag cgcatatgag caaaaaaatg cttaaaatca agaaaaaatt agttgctctt
    12121 gaaatggaac ggtgccagag aaaaattgag cacaaagact gttccaagat tgaccaaaaa
    12181 atcaaagagc agaaggagat atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg
    12241 tggaattact gatttacctc atcctatttt tactggtttt gattgtctca agtacaacca
    12301 ataagctcct gccctttttg cctctccctt tggtgcaaat tcttttggga attgtgattg
    12361 gtctcttttt acccaatact gactttcacc ttaatacgga gttgtttttg gcactggtta
    12421 tcggaccctt gcttttccga gaggctgaag aagcagatgt tacggctatt ttaaaacact
    12481 ggcgaatcat tgtttatctc atatttccag tgatttttat ctcgaccctg agtttgggtg
    12541 gcttggccca tcttctttgg ttcagccttc ccttggcagc ttgcttggct gttggggcag
    12601 cccttggtcc tacggacttg gtggcctttg cctctctttc ggagcgtttt agctttccta
    12661 agcgcgtgtc caatattctt aagggcgaag gactcttgaa tgatgcttct ggtttggtgg
    12721 cttttcaggt agctttgaca gcttggacaa ctggagcttt ttctctgggg caagctagca
    12781 gttcgctcat cttttcaatc ctaggcggtt ttttaattgg atttttaaca gccatgacca
    12841 accgcttcct ccataccttc ttgctaagtg tgcgcgcaac ggatattgcc agtgaacttt
    12901 tattagaatt gagtttgcct ctagtgacct tctttctggc agaagaagtt catgtttcag
    12961 gtattattgc cgtcgtagtt gctggaattt taaaggcaag tcgcttcaag aaaatcacgc
    13021 tcctcgaagc ccaagtggat acggtgaccg agacggtctg gcatacagtg acctttatgc
    13081 tcaacggttc tgtctttgtg attttaggga tggagttgga aatgatagca gaacctatct
    13141 tgaccaatcc aatctataat cctctacttt tattgctatc tcttatcgcc cttacctttg
    13201 tcctttttgt cattcgtttt attatgatct atggctatta tgcctataga acccgacgcc
    13261 tcaagaaaaa gctaaataag tatatgaagg acatgtttct cttgaccttt tcaggtgtta
    13321 agggaacggt gtcgattgct acgattctct tgataccaag taatctagaa caggagtatc
    13381 ctcttttgct tttccttgtt gcaggtgtga cgcttgttag ctttttaaca ggtctcttgg
    13441 tcttgcctca tctttctgat gaagaggaag aaagcaagga ttatcttatg catatcgcca
    13501 ttttgaatga agtaacgcta gagttggaaa aagagttgga agacaccaga aataaacttc
    13561 ccctctatgc ggctattgac aattatcatg gacgtattga aaatctcatt ttaagccaag
    13621 aaaaccagga tgatcaagaa gactgggctg ctttgaaact cttgattctt agtattgaaa
    13681 gtgatggttt ggaacaggcc tatgaagagg ggaacattag caatcgtgct taccgagttt
    13741 accaacgtta tctgaaaaat atagaacaag gaatcaatcg taaacttgcc tcaagactga
    13801 cctattattt tcttgtttcc ttgaggattt tacgttttct ccttcatgaa gtttttactc
    13861 ttggaaagac cttccgtagc tggaaggaca aggagcaaag ccgtctccgt gctcttgatt
    13921 atgaccaaat tgcagagctc tatcttgcca atacagagat gattattgaa agtttggaaa
    13981 acctgaaggg agtctacaga cgctctttga ttagttttat gcaggagtct cgtcttcgag
    14041 aaacaactat tatcagcagt ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca
    14101 acaatatcaa tgaaatgctg agaggctatt atctggagcg caagttgatt ttcgaatacg
    14161 aagaaaaacg attaattaca actaagtatg ccaagaaatt acgacaaaat gtaaataact
    14221 tagagaacta ttccttgaag gaagctgcca ataccctgcc gtatgatatg gtggaattgg
    14281 taagaagaaa ttagttaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta
    14341 ccgtatgtat gtgactgact tcgtcagtct tatctacaac ctcaaaacag tgttttgagc
    14401 agcttgcggc tagctttcta gtttgctctt tgattttcat tgagtataag attataaatg
    14461 aaggagtatg acatgaaaaa gtggtggaaa gagctgatag acaagccttt attaaaagct
    14521 tttttgcatt attatcaagc atcagatagt gagttgacca gtgtcgcagt agcctactat
    14581 tggttgattt cgattttccc cctgcttttg gtggtggtca atatcctccc ctattttcag
    14641 attcctgtgg gagaatttct gggctttatg aaagacgtcc tgcccccaag cctctatgaa
    14701 ggtgtggaaa aaatagccag agaagtcttg acgcaacctt caacaggttt attgagtttt
    14761 tctgttttat cggcgttatg gagtttttca aaatctataa atttcttgca aaaagctttt
    14821 aacaaggctt atggcgtcga aaagagtcgt ggacttattt cccatcagat gctaagtctc
    14881 ctagtcagtt ttggcttgca gttcctcttt gcgtttgcct tgtttttgat cttatttggt
    14941 cagatgattt tggctttact tgctcattat tggacaaaag atggcattat ctatcagact
    15001 ttacaagggt tggcaggtcc tctgatttac gccctcctct ttgctatctt ggtcatgctt
    15061 tattattttc ttcccaatct atctaacagg aaaattagct ataccctacc aggcagtgcc
    15121 tttgttctct tggtgatttt aggtgtacta actctgttct ctagttatct caattactat
    15181 gtccatcatt tggtggatgt ccgaatttta ggttccgttc ttcttgttgt tatgatgttt
    15241 tggtttatct tgattgctaa aattgttatc ctaggcgcgg ttatcaatgc cagtatgcag
    15301 agtttgaaag atccggtctt taaaaaagac taactattta tccattataa aagcgcatag
    15361 tatcaagact ttatagaacc ttgatactat gcgcttttgt gctttgacag ttttttgtat
    15421 agtttttgtc caagatttct aaatcatctg attaaattcc tattttcccc tatcattgtc
    15481 cctgtttttt cgaaaatgga aggagtataa taaatctatc aataaaatga taggaggaac
    15541 tttatgggtc atattttctt ttttctaagt gtctttttgg cagggattct atccttcttt
    15601 tctccttgta tcttaccttt gttaccggtc tatacaggag tgttactaga tgataaggat
    15661 ggtgctcagg cttctagcgg caaattttca atctcagtta ctagtttatt acgaactctg
    15721 gcctttatag caggaatttc ctttatattt attttgttgg gctatggagc tggtttttta
    15781 ggcgatttgc tttatgcttc ttggttccaa tatcttactg gggcaattat tatctttctt
    15841 ggtttgcacc aaatggagat tctacacttt aaggggcttt ataaagaaaa gaggctacaa
    15901 ctgcaaggac aggggcaaaa tggtaagggc tatagtcagg catttttatt gggcttgacc
    15961 tttagttttg cttggacgcc ttgcgtgggg ccggttctgg ggtctgtttt ggccttggcg
    16021 gcttcaggtg gttcaggagc ttggcaggga gctggtctca tgttggtgta tacgctgggc
    16081 ttggcgctac cattcttgct tctagctctg acctctagtt atgttttgaa acatttccga
    16141 aaacttcatc cctatctcgg aatcctcaaa aaagtgggtg gttttctcat tattgtgatg
    16201 ggactcttgg ttctgtttgg aaatgcttca attttaagtc aattatttga ataaaatgga
    16261 aaggaatatc aatatgaaaa aatggcaaac atgtgttctt ggagcaggtt cgctcctttg
    16321 tttgacggct tgttcaggca agtccgtgac tagtgaacac caaacgaaag atgaaatgaa
    16381 gacggagcag acagctagta aaacaagcgc agctaaaggg aaagaggtgg ctgattttga
    16441 attgatggga gtagatggca agacctaccg tttatctgat tacaagggca agaaagtcta
    16501 tctcaaattc tgggcttctt ggtgttccat ctgtctggct agtcttccag atacggatga
    16561 gattgctaaa gaagctggtg atgactatgt ggtcttgaca gtagtgtcac caggacataa
    16621 gggagagcaa tctgaagcgg actttaagaa ttggtataag ggattggatt ataaaaatct
    16681 cccagtccta gttgacccat caggcaaact tttggaaact tatggtgtcc gttcttaccc
    16741 aacccaagcc tttatagaca aagaaggcaa gctggtcaaa acacatccag gattcatgga
    16801 aaaagatgca attttgcaaa ctttgaagga attagcctag gaggtgtctt atgaatgata
    16861 agttaaaaat cttcttgttg ctaggagtat tttttctagc cataaccggt ttctatgttc
    16921 tattgatacg aaatgcaggg cagacagatg cctcgcaaat tgaaaaggcg gcagttagcc
    16981 aaggaggaaa agcagtgaaa aaaacagaaa ttagtaaaga cgcagacttg cacgaaattt
    17041 atctagctgg aggttgtttc tggggagtgg aggaatattt ctcacgcgtt cccggggtga
    17101 cggatgccgt ttcaggctat gcaaatggta gaggagaaac aaccaagtac gaattgatta
    17161 accaaacagg tcatgcagaa accgtccatg tcacctatga tgccaagcaa atttctctca
    17221 aggaaatcct gcttcactat ttccgcatta tcaatccaac cagcaaaaat aaacaaggaa
    17281 atgatgtggg gacccagtac cgtactggtg tttattacac agatgacaag gatttggaag
    17341 tgattaacca agtctttgat gaggtggcta agaaatacga tcaacctcta gcagttgaaa
    17401 aggaaaactt gaagaatttt gtggtggctg aggattacca tcaagactat ctcaagaaaa
    17461 atccaaatgg ctactgccat atcaatgtta atcaggcggc ctatcctgtc attgatgcca
    17521 gcaaatatcc aaaaccaagt gatgaggaat tgaaaaagac cctgtcacct gaggagtatg
    17581 cagttaccca ggaaaatcaa acagaacgag ctttctcaaa ccgttactgg gataaatttg
    17641 aatccggtat ctatgtggat atagcaactg gggaacctct cttttcatca aaagacaaat
    17701 ttgagtctgg ttgtggctgg cctagtttta cccaacccat cagtccagat gttgtcacct
    17761 acaaggaaga taagtcctac aatatgacgc gtatggaagt gcggagccga gtaggagatt
    17821 ctcaccttgg gcatgtcttt acggatggtc cacaggacaa gggcggctta cgttactgta
    17881 tcaatagcct ctctatccgc tttattccca aagaccaaat ggaagaaaaa ggctacgctt
    17941 atttactaga ttatgttgat taatactctt cgaaagtctc ttcaaaccac gtcagcgtcg
    18001 ccttaccgta tgtagggtta ctgact
//
LOCUS       DAVMHG010000032        17723 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMHG010000032 DAVMHG010000000
VERSION     DAVMHG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.032"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(275..1249)
                     /gene="pta"
                     /locus_tag="VYR08_000842"
     CDS             complement(275..1249)
                     /gene="pta"
                     /locus_tag="VYR08_000842"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV3466401.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1293..2189)
                     /locus_tag="VYR08_000843"
     CDS             complement(1293..2189)
                     /locus_tag="VYR08_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3466402.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2186..3004)
                     /locus_tag="VYR08_000844"
     CDS             complement(2186..3004)
                     /locus_tag="VYR08_000844"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV3466403.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(2988..3659)
                     /locus_tag="VYR08_000845"
     CDS             complement(2988..3659)
                     /locus_tag="VYR08_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV3466404.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3787..4356
                     /locus_tag="VYR08_000846"
     CDS             3787..4356
                     /locus_tag="VYR08_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV3466405.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQLTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4469..5428
                     /locus_tag="VYR08_000847"
     CDS             4469..5428
                     /locus_tag="VYR08_000847"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3466406.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5438..6553
                     /locus_tag="VYR08_000848"
     CDS             5438..6553
                     /locus_tag="VYR08_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3466407.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEANNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            6558..6905
                     /locus_tag="VYR08_000849"
     CDS             6558..6905
                     /locus_tag="VYR08_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV3466408.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            6913..7128
                     /locus_tag="VYR08_000850"
     CDS             6913..7128
                     /locus_tag="VYR08_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3466409.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            7211..7852
                     /locus_tag="VYR08_000851"
     CDS             7211..7852
                     /locus_tag="VYR08_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV3466410.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGRALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            7870..8559
                     /locus_tag="VYR08_000852"
     CDS             7870..8559
                     /locus_tag="VYR08_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3466411.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(8556..9236)
                     /gene="radC"
                     /locus_tag="VYR08_000853"
     CDS             complement(8556..9236)
                     /gene="radC"
                     /locus_tag="VYR08_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV3466412.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9279..11570)
                     /gene="pcrA"
                     /locus_tag="VYR08_000854"
     CDS             complement(9279..11570)
                     /gene="pcrA"
                     /locus_tag="VYR08_000854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV3466413.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFFHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESRARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(11761..12564)
                     /locus_tag="VYR08_000855"
                     /pseudo
     CDS             complement(11761..12564)
                     /locus_tag="VYR08_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(12944..13804)
                     /locus_tag="VYR08_000856"
     CDS             complement(12944..13804)
                     /locus_tag="VYR08_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV3466414.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(13820..15097)
                     /locus_tag="VYR08_000857"
     CDS             complement(13820..15097)
                     /locus_tag="VYR08_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV3466415.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(15090..15641)
                     /locus_tag="VYR08_000858"
     CDS             complement(15090..15641)
                     /locus_tag="VYR08_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466416.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(15657..16916)
                     /locus_tag="VYR08_000859"
     CDS             complement(15657..16916)
                     /locus_tag="VYR08_000859"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV3466417.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASIKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         5347 a         3885 c         3235 g         5256 t
ORIGIN      
        1 gcataatagc aggtgtcaaa gcactcaata ttatgcgttt tctcatgaaa aaattctctt
       61 ttcatactca tttaaaatca aaaagggaat ttatcatttt acggacatgt atattacaga
      121 tttctatagg tcgtatatag acaggatatt atcgcttaga agttgtcctt tttgacacca
      181 atcagtaatt tagttctcag gagttaatgg aagtgctttt cgcacatagt ttcataacta
      241 cagtatagca tagtatatca ctttatagtt ttcactattg atgaactgct tgagctgctg
      301 tgatgagggt caacttgtaa acatcgtctg cattacatcc acgagaaagg tcgttaactg
      361 gtttgttcaa accttgcaaa acaggtccta cagccgcaaa accacccagg cgttcagcca
      421 tcttgtaacc gatatttcct gcctcgatac ctgggaagat gaagacattt gcttgaccag
      481 ctaccgtact tcccggagct ttcagagctg cagtttcggg aacaaaggcc gcatcaaatt
      541 gcaactcacc atcgatttca aggtcaggac gcaagtcgtg agcaatttta gttgcttcaa
      601 cgaccttatc aacgctttca ccaaaccctg aacctttagt agaatagctc aacatggcaa
      661 ttttaggttc gatgccaaac atcttagctg tgattgctga gttgatggca atttcagcca
      721 aggcttctgc atctggattg atattaatgg cacagtctcc aaataggtaa cgttccgtac
      781 cacgaaccat gaggaaggct cctgaagtac gagttacatt tggacgagtt ttgatgattt
      841 gtagagctgg gcgaactgtt gaagctgttg agtgaatcgc tcctgacacc attccatcaa
      901 ccaagcccaa gtaaaccaac atcacaccaa agtagttgac atcttcaacc aaaaccttgc
      961 gtacatcttc ttcagtcatt ttgcccttgc gacgctccac caaggcagaa accatttctt
     1021 caaattgagg ataatgttga gggtcgatga cctcataacc atccatgatt ccttcaattt
     1081 caagataaat tttaattttt tcaggatttc caagcaaaac aggaatcact tctgtttctt
     1141 ttactaagcg ttttgttgct tgaagaatac gaggctcttc cccttcaggg agaacgatac
     1201 gagcattttt accaacaagg ttggctttga gactttcaaa aacttccatg agttttctcc
     1261 tttaagataa taattatact ttgagacagt ttttatagag tattagttga taactgggta
     1321 ataaggttac tgaaatcatc cggcaaggga ctttctaact gcaagtcttg ctctaaaaat
     1381 ggatgataaa aggataggta atggcaatgc agagcctgac gttgaatacc atcttccaga
     1441 ctaccaccat acaaatcatc tcccagcaaa ggaaaaccga tatgagaaaa atggactcgg
     1501 atttgatggg ttcgaccagt gtgcaggtga atatagacca agtgaatatt tccataagaa
     1561 gctacaatct tgtatgaagt atgggcatac tttccgcctt tagccactcg tctggtaata
     1621 atggaatctt catcacgcgc aatcggagca ataatttccc cttctggctc caaatgtcca
     1681 tctcccttaa ccaaagcaaa gtagcgtttc tcgatagatt tcttctgcaa ctgcttgtct
     1741 aatcgtgcat gggcataacc gtgcttggca aagagcatca agccagaagt gtccctatct
     1801 agtctggtaa caatgtgaac ctgctgattt tcataatttt gcttgacata gtaacccttg
     1861 ataaaattgg caatggtatt agagtgattg acactaggaa tagaagccac tccatagggt
     1921 ttattcaaga ctagaaagtg gtcatcctca tagagaatat ctaatggatg ctcaatagcc
     1981 tccaaggttt caaagccttc ctcagcggga atgtaaatgg taacgtagtc tccaacgtcc
     2041 aatagatacg ttgcattttg cggttgatta ttgaccagaa tagctccacc tcgaaactta
     2101 atcttggcca gcaatccctt agaaacctcg tgctttttta aaaaggtctt aaccttgaca
     2161 tgttcatctg cgataaattc aaacctcatt catccacctc accgataaag gcatccttaa
     2221 cacggttcca gaaactggta tggctaggag tcgcgacaaa gtgaatctta tgatggtcga
     2281 tttgatactc aatacgctca atattacgga aagaataaac gctattgtca accgaaatag
     2341 tatgataatc gtttcttgtt ggaataagtt caatcttatc cttcttaggc acaataatgg
     2401 aagagcccag tgttcgatag acacgattat taaggctggc aatctccgtt aattgcaaag
     2461 cttcaatggt agggtgtaaa acagcaccgc caagagactt gttataggca gtactaccag
     2521 tcggtgtcga aactgttagc ccgtctccac gaaaacgttc aaagggaaca ccatttatta
     2581 caatatctgc caccatggtt cgatcagacc tgcggatgct ggcttcgttg agtgctctga
     2641 aaatcttaac ttcaccattt tcgagaaaga ccttcacatt cagaacaggg taagaaaccc
     2701 ttgccccagt atctagctgc aaattagtca ctagcttgtc caactcaaaa tcacgataat
     2761 ctgtatagaa gcccaaatgt ccagtatgaa gaccgataaa gcggaccttg tcaagctgat
     2821 tttcgtactt atgaaaggcc gacaagagca taccatcacc gccaatggaa atgacaatat
     2881 ccggattggt atcattgagt ataaactgat ttctcttcaa acgatctcgc aattcataca
     2941 aaaccctttg actctgcggt tttctattgg ctatcagatc aattcgttta cctgtattct
     3001 tcatctgtat cgtcactgtt tcctacaccg tcatttaatt ttctactcaa aggatcaaaa
     3061 agtgcctggg cttcttggat atcatcacga atttcaccca tttcttcatc caactgatgg
     3121 gcgattctag ctgtaatttc cagtcgcttc ttaatctcat ctgggaaatc cccttggtac
     3181 ttgtagttga gagaatgttc tatcgttgcc cagaaattca tggccaaagt acgaatttga
     3241 atttctgcca aaatagtctt agctccattg atggtatcaa ccgtatattc tactaccaca
     3301 tgataggaac gatagcctga tgcttttcta tgagtaatgt aatctcgctc ctgtatgatt
     3361 cgcatatcct gacgcttgtg caaaatatcc actacttcct tgacgtcatc tacaaactga
     3421 accatcacac gtaagccagc aatatcctgc aaatcgtgtt ccaaggtcgc ataagtaatg
     3481 ccacgacgag ccattttttc tttgatgctc tcaattggct tgactcgacc ggtcacaaac
     3541 tcaattggag aatgcttatt ttgcttacga tattgcttac gaataccacg aagtttaatc
     3601 tttaactcac caacagcttg aatgtaagga tctagaaatt cttcccattc taaggtcata
     3661 tattctccct tgttctcata ttatccgtca aaaatatctt tgattttttc tccagattca
     3721 tatacaataa atacaatgct tattatacca taaatccgct ttcaacgata aaaaaaggta
     3781 agaaaaatga aacatttaga aattgaattg aaaacactat tgaaaaaaga tgaatacaat
     3841 cgtctaaaag accagctcac aggtgtcact cctgttcttc aaacaaatta ctacatcgac
     3901 acgcctgatt ttgaactgcg agaaaagaaa gtcgctatgc gcattcgaac ctttgaagac
     3961 tgggctgagt tgacactcaa agtcccgcag agtgttggaa acatggaata caaccaaaaa
     4021 ttgcaactaa aagatgctga gaactatctg agtaaggaag aacttcctca agggctagta
     4081 ctggatgaat tggcgaaaca tggtatccaa agtaagaact ggcaggttct tggttgtcta
     4141 acaacgcttc gctatgaaat gaaaacagct attggtctca tggcactgga tcagagccga
     4201 tactttgata taacagatta cgaattggag cttgaagtgg aaaatcatga gcaaggcaaa
     4261 caggatttcc gacaattttt agagaaaaat cagatttcct accaaaaagc tccttcaaaa
     4321 ttggttcgat ttgtcaaaag tatgaaaaat agctgaaata atctccattt tttggtaaaa
     4381 tagaaaagat aaaaatacac aaatcccagt tcatatatgt aaggtaaata taactaggat
     4441 ttataaagtt tacagaggac ggtctataat gtcagataga aaaaacatga aacttttcgc
     4501 actcaactct aaccaagaga ttgcacaaaa aattgcccaa gctgttggtg tcccacttgg
     4561 aaaactatca tcacgtcaat tttcagacgg agaaatccaa gtaaatatcg aagaaagtgt
     4621 ccgtggttat gatgtttaca tcatccaatc aacaagtttc cctgtcaaca accacctaat
     4681 ggaattgtta atcatggtcg atgcttgtgt gcgtgcaagt gcccacagta tcaacgttgt
     4741 ccttccatat tttggctatg cacgtcaaga ccgcattgct tgtcctcgtg agccacttac
     4801 agcaaaacta gttgccaata tgctggttaa ggctggagtt gatcgtatcc tgactcttga
     4861 tttgcatgcc gttcaggttc aaggtttctt tgatattcca gtggataatc ttttcactgt
     4921 tcccctattc gcaaaacatt actgcgataa aggattgctt ggttcagatg ttgttgtcgt
     4981 tagccctaaa aattcaggtg tcaaacgtgc gcgtagcctg gctgaatatc ttgatgctcc
     5041 tatcgccatt atcgattacc ctcaagacga tgcaactcgt aacgaaggtt atattattgg
     5101 tgatgttgaa ggtaagaaag ctatcttgat tgatgatatt ttaaatacag gacgtacctt
     5161 ctctgaagct tctaaaatcg ttgaacgtga aggagctaca gaaatttatg ctgtttctag
     5221 ccacggtctc ttcgtcgagg gagctgctga gcttcttgac aatactaata ttaaagaaat
     5281 tcttgtgact gattcagtag caacaaaaga aaaaactcct aaaaatgtat gctacatcac
     5341 tgctagtgag ttaattggcg atgctatcgt ccgtattcac gaaagaaaac cagtcagccc
     5401 actctttgcc tacaataaaa agaaataagg tgattctttg atttatttgg acaatgctgc
     5461 aacgactcct atgtcagcag ttgctatttc agctatgacc aaggttatgc aagaaaccca
     5521 cggaaatcct tctagtattc atggtcatgg tcgtcaagct ggcaaactct tgcgagaagc
     5581 ccgtcaggaa ctagcccagt tactaaggac aaaacctcaa catatctttt tcacttctgg
     5641 tgggactgaa gccaataata ctaccatcat tggctactgt cttcgtcacc aagaacaagg
     5701 aaaacatatc atcacaactg ccatcgagca ccatgctgtc cttgaaacaa ttgattactt
     5761 ggttcaacac tttgggtttg aagcaaccat tatccagcca gaaaatcaag aaatcacagc
     5821 ccagcaaatt caaaaggctt tacgtgacga tacgattttg gtttctacca tgtttgccaa
     5881 taatgagaca ggaaacctac tgcccatcgc tgaaattggc caaatactca agcaacaccc
     5941 tgctgcctat catgttgatg cagttcaggc tattggtaaa atcccaattc attcagaaga
     6001 attgggcatt gattttctca ctgcttctgc ccacaaattc catggtccta agggaatcgg
     6061 ttttctctac gcatctagca tggactttga ttcctatcta catggcggag accaggaaca
     6121 gaaaaaacgt gcaggaacag aaaatctgcc tgccatcgta ggcatggttg cagccctaaa
     6181 agaagaccta gaaaaacaag aagaacattt tcaacatgta caaaatctag aaactgcctt
     6241 tctggcagag ctggagggca ttcagtatta cctgaataga ggaaaacatc atctccctta
     6301 tgttctcaat attggatttc ctggtcagaa aaatgacctc ttactccttc ggctagattt
     6361 agctggaatt tcaatctcta ctggctcagc ctgtactgca ggcgttgtcc aatccagcca
     6421 tgttcttgaa gccatgtatg gcgcaaattc agaacgcttg aaggaatccc ttcgcatcag
     6481 tttgtcgcca caaaataccg ttgaagacct acaaaccctc gcaaaaacct taaaagaaat
     6541 tatcggaggt tagccatatg gcatttgaaa aaatcattca gttaaaaaat tgtcgttacg
     6601 attacactct tagcccttct gttaaaaaat tcaccctcaa agataacacc ttttttgaaa
     6661 ctaaggttgg taactatgaa ctgactcgcc ttttggaaaa agtgccaaac agcggtgaag
     6721 gcttccaact caaaatcatc attaacaagg aacttacagg ggctaaaatc aatatcactg
     6781 acaagtttgg ccttcgtcta gttgatattt tcaaatcaga agaccaccat attcatcagg
     6841 aaaaattcta cttcctcatg gatagcttgg tagaacgtgg tgtctttaca aaatcggaaa
     6901 gatagagcca aaatgtttga actgacctat aaggactgct atcatgtaga gcgtactctc
     6961 aagtatgagg atcatgaggc tctcatgttg actttgtcag gatgtgtaac cttaccagat
     7021 acactttatg tgacttctct gacttttagg ggccaaaaag tttatcaggg tctggtcgga
     7081 gacctctacc gttttctatc tcatgcagat tttttacatc aaaactaaga tttttcttag
     7141 ttttttctta tttttgttga ttttttcaca atgtttctat aaaatagaag aatagaaagg
     7201 ttgtgatttt gtgaaagata aacagtttgc tattccaaaa gctacagcaa aaagactctc
     7261 tctctactat cgaattttta agagatttca tgcagaaaag attgaacgtg ccaactctaa
     7321 gcaaattgca gaggctatcg gtattgattc agcgaccgta cgtcgtgatt tttcctattt
     7381 tggtgaactt ggtcgtcgtg gttttggcta tgatgtcaaa aaactaatga cattttttgc
     7441 cgatttgctc aatgacaact ctattaccaa tgtcatgctg gttggtattg gaaatatggg
     7501 ccgtgccctt ctccactacc gcttccacga acgtaacaag atgaagatta tcatggcctt
     7561 tgacctagac gaccatcctg aagtcggtac ccaaactcct gacgggattc ccatttacgg
     7621 gatttctcag atcaaggata aaatcaagga tgctgatgtg aagactgcta tcctaactgt
     7681 tcccagcgtc aagtcacaag aggttgctaa tctcttggtt gatgctggtg tgaaaggaat
     7741 tctcagtttt tcaccagtcc atctgcattt accaaaagac gtggtcgttc agtatgtcga
     7801 tttgacaagt gaactccaaa ccctcctcta cttcatgcga aaagaggatt agaaagcgaa
     7861 aatcatttta tgaaaaaacc agttattggg attacaggaa acgaaaaagc tcatccagat
     7921 gatgacatca tgatgagcta cgcagcaaaa ggctttgttg aaggcgttaa agatgctgga
     7981 gggattccca tcatcctacc gattggtgat caagaaatgg cctgccacta tatcagtttg
     8041 attgacaagc tcatcttgac aggtgggcaa aatgtcgatc caaaattcta tggcgaaccc
     8101 aaaaccattg atagcgatga ttatcacctt caaagagata tcttcgaact ggccctcatc
     8161 aaggaagcta ttaaacagaa aaagcccatt ttctctgtct gccgtggtac ccaactcttt
     8221 aacgttgcca tgggtggaac tttgtaccaa gatatcgaag accattggca ggattcttct
     8281 gttgagtaca caactcaacg cttggtaaca gaaccagata ccgttctcca agaaatctat
     8341 ggagaaatct ctcatatcaa ctccttccac caccagagca tcaaggactt agcaccaaat
     8401 ttaaaagttg tggctcatga tcctaaagat ggtatcattg aggctgtcat gagtacggat
     8461 gatgtcgcct ttctcggtgt ccaatggcat ccagaacttc tttttgaaaa tcgtcccaaa
     8521 gataagaaac tctttgacta tgtcgttaat gaactttaga ttaaatctgt cttttcacga
     8581 taactaaagt aattagaatg agagacaatc aaatggtcca agagaacaat ccccatcagt
     8641 tcgcaggctt ctttaacaag tttagtgaca tgatcatcat tttggctagg cgctaccgct
     8701 cctgaaggat gattgtggac caagataaga gaagtcgcca tatgcttgat tgcatagtgc
     8761 agaatctctc gcggttcagc gatactacga gttacagaac cgataaaaat ggtctgctga
     8821 tggatgattt gattttgagt attgagatag agtgccacca ggtgctcttg ttttttatcc
     8881 cctaattcct gctgcatctt cttggccaac ttttgactgc tgagaatact ttccatttca
     8941 agagtctcgt gtttgtgaat acgatgcccc agttcaatca tagcttgtaa ttctatggcc
     9001 ttaacacgcc caataccaga caaactctgc aattcctgca gggtcatttt tttcaaatcc
     9061 gttaggcttg aaagattgtt caagactttt tgggcaattt caaaaacgct agcttgacgt
     9121 gttcctgtcc tgagtaaaat agctagcaac tcttggttac taagcgcttc aactccttcc
     9181 ttggccagcc tttctcttgg taatagtgaa tcttcttgga atgaaatact gtacataaaa
     9241 atcctcctca ctttattatt cgtgagaagg atggaaaatt agattttttt ctcaattgga
     9301 gccacactgg ctaaaagttt tttcaaacct acttctggga aattgatttt caattcctgc
     9361 ctagcaccgc tacctgaaac ttccagaaca gttccctctc cccatttctt gtggagagca
     9421 atgtcaccaa tggaccaatt tgcctcgcta gatgctgatt ttgcgccagc tgtaaattga
     9481 ccaaatggaa gaccgcttga ctggattgat tttggggcag caccgcgttt acggtcttga
     9541 agagcctgag ccaaactcat accttgacca aaggaaatac taccactgct atatgatgcc
     9601 ttaaagcttg tatttgcagg acgagccaga ccttgatact caagcaagtc tgaactgatt
     9661 tcgttaataa aacgagtcgg acggttataa ttggtacgac caaaaagcaa gcgtgagttg
     9721 gcattggtca gatagagaat tttctctgca cgcgtgatac ctacataggc tagacggcgc
     9781 tcttcttcta attcatctgg atcttcagtc gcacgactaa gtggaaagac attttcttcc
     9841 atcccaatca aaaagacaac tggaaattcg agacctttgg cagcatgcag ggtcatcaag
     9901 gtcacttctg atgtctcctg actacctgaa tctgtgtcgg caatcaaagc caagtcattt
     9961 aagaaacgac tcagtttgtc cagaccagtt tcctcttctg tcacatcagt ggtgtcatca
    10021 aagttcttcg taacagaaag aaactcttcg atattttcaa cccgtgccct gctttctaga
    10081 gtcgcttggg cgttaagaat atcgacataa cctgtttttt ctaggacgga ctcaaccaac
    10141 tctgtaatgc ttaagtggtc tagctgctcc cgcaaatcaa gcatcatatt ggcaaaatcc
    10201 cagatagatt gggctgcctt acccttaata ccagacaaca taatattagc agaagcatcc
    10261 agcatggaca tattttgcaa atttgcaaaa tcacggattt tctcaactgt acctagacca
    10321 attccacgtt taggctcgtt gataatacgc tcaaaactaa tattgtcact caaattagca
    10381 ataaggttga gataagcaat aatatcgcga atttccttac ggctgtagaa tttggttccg
    10441 ccaaccatgg tataaggaat gttagacttg agcagggctt cctcaattgt acgggactgg
    10501 gcattagtcc gatagagaac tgcaaaatcc ttatgaaaga agttttgact gcgactaagt
    10561 tcatcgatgg ttctggctac aaatacagcc tcatccagct catcatcggc acgatagtaa
    10621 acgatttgct ccccatcagc gttttgagtc cagagatttt taggacggcg atttttattg
    10681 tttttaataa cctcgttggc cgcttggaga atggttttgg ttgagcggta attttcctcc
    10741 aacaaaacaa ccttggcttt ggggtaatcc ttttcaaagt ccaagatatt ctgcatatca
    10801 gcaccacgcc aaccgtagat agactggtcc gcatccccaa ccacacagat atttttaaaa
    10861 cgggaagcca agagtttgac caattggtac tgagcgtggt tggtatcttg gtactcatca
    10921 acgtggatgt attggaattt ttgctggtag taggtcaaaa catcaggatt ttgatcaaag
    10981 agacgcaagg tcagcataat caaatcatca aagtcaacgg attcggactg acgaagttct
    11041 ttttgatagg ctgtataaca ctgggccaca atttgcgtat acatatcgcc agcttgggca
    11101 gcataagcaa catcatcaat caaatcattc ttagcattgg aaatggtccc caaaatagtt
    11161 cgttcattcc attttttagg atccaagttc aactgtttta gaatacgttt catgagcgtt
    11221 cgctgttcac caggatccac aattgtaaaa ttacgattgt agccaatatg gtccgcatct
    11281 cgacgcaaaa tacgcacaca catggagtgg aaggtcgcaa tcagacagtc ctgagtagct
    11341 ggattgaggc tataagcacg ctctttcatc tcacgcgcag ccttgttggt aaaggtaatg
    11401 gccaagatat tccaaggatt gaccagcttt tcatcaatca aataagcgat acggtgggtt
    11461 aaaacacgag tctttccaga accagcccct gccatgatta gcaagggacc ttctgtcgtt
    11521 tgcaccgcct cagcctgacg gtcattcatt ccatttaata atgcgttcat cttctcttcc
    11581 ttactcttga agtcaatatt tctattatat cagaaatcag ggaaaataga aactttacgg
    11641 ggagtagcaa aatatagtgg attgaaacta gaatactaca ctgtgggttt ctaaaacact
    11701 cgacttttca cccaattttt tcataaacta gctcaatacc tcaatagact tcctgcaaaa
    11761 ctagaatcct agttcatgat tgataatacc agcaatcaaa ttcattcgta atccgaagcg
    11821 tttacgatga tttcgataag ttgttgaaaa cattttaaac gtttttactt tggcaaagat
    11881 gttctcaacc ttgcttctct ccttagatag cgcatggttt aagccttatc ttcaactgtt
    11941 agcggcttga gtttgctgaa tttacgtgga gtttgtgctt gaggatatat cttcatgagc
    12001 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct gcgactcatt
    12061 ttgaacaact tcatatcatg acaatagttc acagtgatat ccaaagaaac aattcttcct
    12121 tgacttgtga caatcgcttg agccttcata gcgtgaaatt tctttttacc agaataattc
    12181 gctaattctt tttttagggc gattggtttt tacttccgtc gcatcaatca ttaccgtgtc
    12241 ctcagaactg agaggagttc ttgaaatcgt aaaaccactt tgaacaagag ttacttcaac
    12301 ccattggctc cgacggatta agttgctttc gtgaatacca agatcagccg caatttgttc
    12361 ataagttcga tattctcgca catattgaag agtggccata agaaggtctt ctaggcttaa
    12421 tttaggtttt cttccacctt ttgcatgttt aagttgataa gctgttttta atacagctaa
    12481 catctcttca aaagtggtac gctgaacatc aacaagacgc ttaaatcgtg catcagttag
    12541 ttgtttactt gcttcatcat tcatagaact actataccat gttttgtttc gcaggaagta
    12601 taatataaaa aaataaattt aagattggca atacgaacgt taaacagaat tccatttctc
    12661 tttttagaat caaaacgaca atattgtgaa atctctacag gaacaataga ctgcgacact
    12721 aaaatatcct cccttagttt tactttacta atagtatata ggaggagagc cctgaatcct
    12781 agaccctttg ttatgatgcg cttacaaaca gagttggaaa aaatcttaaa agcagaaaaa
    12841 cgcatgatat cagcccctaa cacttgatac aatacgtttt attatgaaaa gatctactgg
    12901 attttttatc aaaataaact tccagtagtc ttttcacttt ttattaataa gttccttctt
    12961 caccttggct agtcaagata acaggtccat ctttcgtaat gacaaattgg tgttcatact
    13021 gacatgacaa tccaccgtca atggtcttat gcgcccaacc agttttcata tctgtatcaa
    13081 tttcccaatc gcctgtattg atcattggtt caatggttaa gaccattcct tcacgaagac
    13141 ggagtccacg acctgcaata ccatagttag gaaccattgg ttcttcgtgc atagttgggc
    13201 caacaccatg accaaccaaa tcacgcacta caccgtaacc acgactttca gcgtattctt
    13261 gaatagccgc accgatatca ccgatacgat ttccaacaac agcttgctca atacccttgt
    13321 acatagcttc tttggttaca tccatcaagt ttttgacttc ttcggacggt gtaccaacag
    13381 cataagccca acatgagtct gctaaaccac cagaatagct ctgagtatat tttttcattt
    13441 gttcaacatt gttgaagttt aattttgaga cattcaggtc agatttagca atgggacctc
    13501 ccaaaaccat atcaactttg agcaaatcac catctttcaa gatataatga cgagggaaag
    13561 cgtgagccac ttcatcgtta agagagcaac aggtagcata aggatagtcc atcatggcac
    13621 cgtcaacccc aatctgaagt ggaaggaaat tttcttcttt acaacgacgg cggacatatt
    13681 cttcaacttc ccacatatct acgcctggct taatcaaatc acgtaagcct atatgaatac
    13741 ttgctagaaa atcaccagcc ttgtccatag cttcgatttc acgagctgat tttaatgtta
    13801 tcatcttttc tcctagtttc taattaattt taacagtcac atttgctttt gaaacaatct
    13861 ggtgaccatg ataaatatcg taatcaatta tagctgaccg tctcgtatga tgaataatcc
    13921 gtgcctgaat gcgcaatata tcatctatct gaacagcctg caaaaagtag atcagcatct
    13981 gctcgataat gagattgcga ccactattaa caactaaatc ttgggtcatg tgggtcagaa
    14041 tttctgccaa tacaccatta gccaaaactc catttttttc tagcataaag ggttccactg
    14101 taatgactac ttcatcatgg tgataagaga gcttttgtcc aatctgctca gaaaaagttg
    14161 gtagagccga aacttgggaa cggctcatct tctccatgac atctcgtcgc gtcacaacgc
    14221 caagcaaagt ttgattgctt cgaacaactg gtaccatttc aaagtcttct gcgatcatcc
    14281 gttgactcac attggcaata tttgtcgata atccaaccaa aaatagacta cgagacataa
    14341 ccttatcaat tgtcgtgctt ggtgatttat caccagcgtc tctcatggtt acaacgccaa
    14401 caacgacctg atgttgattg ataacaggga aacggctgct acgattctta cgaaccaagt
    14461 ccaaataatc tttaactgta tctgtctctc tcagaaaacc atactcatga ctagggcgat
    14521 aaagtttctc aactgtcaga atatcagtct tgatttggac atttgacaag gctttattga
    14581 tcatggtcgc gacggtaaag gtatcatgct tacttcttag aacaggaatc cctttttgat
    14641 tggccagttt aagcacatca tcatgaacct gaaatccccc tgtaaccaga actgcatttt
    14701 cattttccaa ggctagcaac tgaatacggg ttcggtctcc gacaatcaag agccccccat
    14761 catgaaggta agacaagata ttttgttcag tcatggcacc aattgaaaac ttactaaatt
    14821 ctctctctaa accttcttgc ccagccagaa cctcagaaga agtcacttct gcaatttcag
    14881 caaacgttaa tctctctata gcaactttct gggatttaac acgaattgtt ccacttctag
    14941 gacgggtctc cacaattcca cggttttcag cttctttaat agcccgatag gctgttccat
    15001 cactaactcc tagatgattc gaaatgctac gaacactgac ccttttacct actggtaatt
    15061 cctccaaata gcttagaatt tcctgatgct tactcattga actctccttt tacataccga
    15121 aattcaagat aatcccgcat ttttcttgtg taacgatcac ttcccttaaa ctgacggaac
    15181 aaactaaatc cagacttaag agcaactctt tggctagctt cattttcaag gtgggtaatg
    15241 atagataatt gttttaagcc aaattcctca aaagaaagct gacaaatttt tctaacaacc
    15301 tctgtcataa atccttgcga ccaagcatct tttctcaaaa aatagccaag ctcagcttct
    15361 tttttgattt catctaactt ctcaaattta atagaaccaa tcatttgttg atttttctgg
    15421 tcacaaattg cccacactcc caaaggggac ttcataaagt aattggccag tgcatattga
    15481 ctttcttcca gacttgcctg cgttgggaaa ataaattgaa gattttctgg atttgaagct
    15541 atctcgcgga agtcctgact atcactaaaa aagaaaggac gcaaatacaa gcgatccgtt
    15601 tcaaaaaaag aaaacattgc taatttggtc caaatattca taaacacctc tacggtttaa
    15661 tcctcaacaa gtctaatatc cgctcctaaa ttacgtaatt tttcgataat atcagaataa
    15721 ccacgtaaga taaactcgat attggtaatt tcagttttac cttcagccat aagcccagca
    15781 atgactagtg cagccccagc tcttaagtcg gtcgctttaa tactggcccc acgtaaatca
    15841 cgtccacccg tgtacaaaat atgaccattt gttgtcgaaa tatccgcatc catctttgct
    15901 agttcaaaaa catgatttac acgtttttcg taaatcgtat cgacaattgt accacgacca
    15961 ttcgctctta gtaaaagagg ggtaagcggt tgttgcaaat cagttgcaaa gcctgggtaa
    16021 ggagctgtct taatattgat tgctttcaaa ttagactgtt cctcgacaaa aatgctgtct
    16081 tcagatacag tcattctcac tcccatttct tccaacttag caataaaccc ttccaggtgt
    16141 tcgtaaagaa cattatttat acgaattcct ttaccaactg cagcagctaa agatatatat
    16201 gttccagctt caatgcggtc tggaatcacc tgatgacgtg tcccatgtaa tctttcaaca
    16261 ccatcaataa tgatgatatt agttcctgcc ccacggatat gggcacccat attattcaag
    16321 agagtagcta catcaataat ctcaggttca cgggctgcat tttcaataat agtacgacca
    16381 tttgctttaa ccgcagcaat catcgtatta atcgttgctc ccacactaac cgtatccatg
    16441 taaatacttg caccatgaag tcctgtatct ttagcagata acttcatgtt atctccctcg
    16501 tagctagcag tggcacccat agcttcaaac gccttaaggt gtaagtcaat cggacgagga
    16561 ccaagatcac atcctcccgg tagaccaact gtcgcttcac caaaacggcc taagaggctc
    16621 ccataaaaat agtaagatgc acgaagactg ttaattttac cataaggcat tggaatattt
    16681 tgaacacctc ttggatcaat ctccaagaca tcgtcataac gcttaacagt agctcccatt
    16741 aattccatga tttcgacaag actggctaca tccgaaatat ctggaacaca atccaaagtc
    16801 accacatcat cagccaatat aatagctgga attaaggcca caacactatt tttagcacca
    16861 ctaatagtaa tttcaccttg cagtggtaat ccaccattga taacaatttt tctcattcta
    16921 agttttcaat actctttcaa gatttctaat aaggtcttca aaataaagta tatcataaat
    16981 ttatcctttt ttccatcctt ttcaatttta ttggataaat cagagttgac gagttagtaa
    17041 ctgaattact aatactcaat gaaaatcaaa gatcaaacta ggaaactagc cgcaggttgc
    17101 tcaaaacact attttgaggt tgtagatgga actgacgtag tcagtatcct acacatacgg
    17161 caaggcgata ttgacgtgat ttgaagagat tttcgaagag tataatcttt ctcgaatcgc
    17221 tattgcccct tttttatccc ctaaaactga gtaggatcta agactagatc tcttatctaa
    17281 gtatcggaat acggctgttc aactaaattt tagcaagaac ttctcccctt ctctcagttc
    17341 acagaagcgt tttatacata ccgtcctatc agacaatcta ttttaaagat aaccgtgact
    17401 caggatcact tctatctgtc gttttctctt tatagtgata gacctcacgg gataagtcgt
    17461 acatctttcc tcgtgtatac aagatataag gacgttaaaa ggttacagtg aaaatagaaa
    17521 atcttagtac gaattcaatt actctcgtga tttacgcata taggttacga ctaccttttt
    17581 ggactttaga gtttgagacc cccttatccg ctatatacgc cttactatca cgttcctgtt
    17641 cgtaaagcca cgatttcgct atcccttcct ctgacgattc ttatttccag cgtcagactt
    17701 aaaacggtct atccccagac cgt
//
LOCUS       DAVMHG010000033        17376 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMHG010000033 DAVMHG010000000
VERSION     DAVMHG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.033"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..327)
                     /gene="pmp23"
                     /locus_tag="VYR08_000860"
     CDS             complement(<1..327)
                     /gene="pmp23"
                     /locus_tag="VYR08_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV3466418.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGV"
     gene            complement(327..1301)
                     /locus_tag="VYR08_000861"
     CDS             complement(327..1301)
                     /locus_tag="VYR08_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV3466419.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(1309..2565)
                     /locus_tag="VYR08_000862"
     CDS             complement(1309..2565)
                     /locus_tag="VYR08_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV3466420.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEDLAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(2627..3058)
                     /locus_tag="VYR08_000863"
     CDS             complement(2627..3058)
                     /locus_tag="VYR08_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466421.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAVNHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(3060..3662)
                     /locus_tag="VYR08_000864"
     CDS             complement(3060..3662)
                     /locus_tag="VYR08_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV3466422.1"
                     /translation="MMDRIRQELEKGGAVILPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEV"
     gene            complement(3646..4485)
                     /gene="prmC"
                     /locus_tag="VYR08_000865"
     CDS             complement(3646..4485)
                     /gene="prmC"
                     /locus_tag="VYR08_000865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV3466423.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(4485..5564)
                     /gene="prfA"
                     /locus_tag="VYR08_000866"
     CDS             complement(4485..5564)
                     /gene="prfA"
                     /locus_tag="VYR08_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV3466424.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(5574..6053)
                     /locus_tag="VYR08_000867"
     CDS             complement(5574..6053)
                     /locus_tag="VYR08_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466425.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(6058..6642)
                     /locus_tag="VYR08_000868"
     CDS             complement(6058..6642)
                     /locus_tag="VYR08_000868"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV3466426.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKE"
     gene            6765..6947
                     /locus_tag="VYR08_000869"
     CDS             6765..6947
                     /locus_tag="VYR08_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV3466427.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            7084..8457
                     /gene="mnmE"
                     /locus_tag="VYR08_000870"
     CDS             7084..8457
                     /gene="mnmE"
                     /locus_tag="VYR08_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV3466428.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDATPDELITQLF
                     SQFCLGK"
     gene            complement(8504..9439)
                     /gene="dapA"
                     /locus_tag="VYR08_000871"
     CDS             complement(8504..9439)
                     /gene="dapA"
                     /locus_tag="VYR08_000871"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV3466429.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLDHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKIVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLVYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFIAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(9495..10571)
                     /locus_tag="VYR08_000872"
     CDS             complement(9495..10571)
                     /locus_tag="VYR08_000872"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV3466430.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKT
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGAKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(10913..10985)
                     /locus_tag="VYR08_000873"
     tRNA            complement(10913..10985)
                     /locus_tag="VYR08_000873"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10950..10952),aa:Thr,seq:ggt)
     gene            complement(11085..11594)
                     /locus_tag="VYR08_000874"
     CDS             complement(11085..11594)
                     /locus_tag="VYR08_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV3466431.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(11592..11688)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            11717..12148
                     /locus_tag="VYR08_000875"
     CDS             11717..12148
                     /locus_tag="VYR08_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV3466432.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            12230..12607
                     /gene="mscL"
                     /locus_tag="VYR08_000876"
     CDS             12230..12607
                     /gene="mscL"
                     /locus_tag="VYR08_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV3466433.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(12649..13743)
                     /gene="hemH"
                     /locus_tag="VYR08_000877"
     CDS             complement(12649..13743)
                     /gene="hemH"
                     /locus_tag="VYR08_000877"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV3466434.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            13898..15121
                     /gene="pepT"
                     /locus_tag="VYR08_000878"
     CDS             13898..15121
                     /gene="pepT"
                     /locus_tag="VYR08_000878"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV3466435.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(15157..15786)
                     /locus_tag="VYR08_000879"
     CDS             complement(15157..15786)
                     /locus_tag="VYR08_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466436.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLEPTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            15751..15972
                     /locus_tag="VYR08_000880"
     CDS             15751..15972
                     /locus_tag="VYR08_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466437.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(16109..16318)
                     /locus_tag="VYR08_000881"
                     /pseudo
     CDS             complement(16109..16318)
                     /locus_tag="VYR08_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(16439..16581)
                     /locus_tag="VYR08_000882"
                     /pseudo
     CDS             complement(16439..16581)
                     /locus_tag="VYR08_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(16615..16755)
                     /locus_tag="VYR08_000883"
     CDS             complement(16615..16755)
                     /locus_tag="VYR08_000883"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3466438.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(16799..17359)
                     /locus_tag="VYR08_000884"
                     /pseudo
     CDS             complement(16799..17359)
                     /locus_tag="VYR08_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5057 a         3671 c         3265 g         5383 t
ORIGIN      
        1 tacacccttc ttctgcgcca gatagagatt gcctgtcaga gtttgaatgc cttgccgaat
       61 gctagaggca ttatcattga tggtgttggt ggaaccactt gcagactcac tagactgcat
      121 aacatcgcct tcttttcctt ttgtttcagt ataaatcata gcaagcacaa gctcttcgtt
      181 tgctggggtg tctttttcac tcaagatttc tcgcaccatg ggttgatagg tcatgacttg
      241 tttgacatct tgatgaacgc ggtaagcttt atagccagca aaaaggaaga ctgctagtac
      301 aagcactctt cgaattcgtt taaacattat ttactttgga tatcctcgat atttttgatt
      361 aagatagagt aggttccatt ttcgttttgg ataaactcaa cagactcggc gtcttgatag
      421 acgttattgg gaacgatgag ctcaattcca tttgataagg agagtttttg gttttcaaat
      481 ttctttaatt ggcgacttgc atcaatttca tcaaattgaa caggttctgg tacggcttct
      541 ttgacttggt caataaagct caaacgagcc gtcagattgt tgtcaaaaag gtcattagcc
      601 aatttctcag gtgacaattc attgctttct tctaggttgt tgaaaatcgc tgatttgacc
      661 ttggattgaa attgaaaatc atctgtgtta aaagattcag caattctctg ggctgttttt
      721 tccagttcct tgatagattt tttaggagaa atcttaggag cgacagcaag aagattatct
      781 gaaaaatagt tcaaaaaagt cccgttgtac ttgattcgtt tttcaatcag gtgatacttg
      841 cgactctgaa gattgaccac caaggcctcg tcagcacccg ttccaaatcc aggcaggtta
      901 ttctgagtca gcttgattgg attatcaact tctcctccga ggtgggtcaa ggtctcccgc
      961 agggcaattc gcaagaaagc gaaatgttct acaccttctt tagaaaattg aacaaaaatc
     1021 aagtcattgg tcttgagatt ttcagaaatg ctaaactcct ctttccagag attagccagc
     1081 gttactgatg tctccaacaa atcgtctgta atatgattga agaagggatt ttcttcttcg
     1141 aaaatcccag tcttggcttc atctgaatac acatgttcaa tttttttacg taggtattct
     1201 tcgatttttg gagtaatatt gagaaactta tctgctaaga acagctcggt atcatccgga
     1261 ctgaactggt gaataatggc tttcttaata taaatgtcca taaaagtttt attcctcgta
     1321 taatgggaag gcatctgtca attctttgac tgcacttctc acttcttcta atacagcctc
     1381 attttctgaa ttcttaaggg ttttaatgat gagttcagcc actttgcgac tttcttcttc
     1441 accaaatcca cgtgcagtaa tagctgctgc tccgatacga atcccacttg tcttgaatgg
     1501 tgacaaggtt tcgtaaggga ttgaattttt atttagggta atattgactt catccagcaa
     1561 gttttgagca acttttccgt tttctacaac cttagtcaca tcaacaagga agagatggtt
     1621 ttcagttcca cctgaaataa tacggaaatc agggtcttgc aagaagacat ctgccatagc
     1681 cttgctgttc ttaattacat tggcagcata ttccttgaaa gctggatcca aaacttcttt
     1741 gaaggaaact gccttagcag ccacaacatg ctccaaagga ccaccctgaa taccagggaa
     1801 aatagctgaa ttgattttct tagctaaatc ctcatcattg gtcaaaatca aaccaccacg
     1861 aggaccacga agggttttgt gggtcgttgt tgtagtgata tgagcgtatg gcactgggct
     1921 tgggtgaaga ccagctgcaa ccaagccagc gatgtgggcc atatcaacca taagcttagc
     1981 cccaacagca tctgcaattt cacggaattt tgaaaagtca ataatttgag aataggctga
     2041 agcaccagct acaatcagtt ttggttttac ttcttgggct tgtttcaaga tagcatcaaa
     2101 gtccaaaagt tccgtttcag gatccacact ataggaaaga aagttgtagg tttgaccaga
     2161 gaagctaact ggagctccgt gggtcaagtg tccacctgct gccaaatcca ttcccataac
     2221 cgtatcacct ggctcaatca aggacatgta agccgcacag ttagcttggc ttcctgaatg
     2281 tggttggaca ttggcaaatt tagcaccgaa aatttctttt gcgcgttcaa tagcaagagt
     2341 ctctacaacg tctactacat cagttccacc ataataacgg cgtcctgggt aaccctcggc
     2401 atatttattt gtcaagatag acccttgagc tgccataaca gccttggaaa ctacgttttc
     2461 cgaagcaatt aactcgatat tattttgttg gcgttcttct tctttggcaa tagcattcca
     2521 gagatcagca tcatatgctt taaaatcatc tttgtcaaaa atcataggtc ttctccttta
     2581 ttgtgtgact agtccattag tttgatttta caataagaaa atcaaactaa aagatgcgaa
     2641 taaaccgttt ctgcatttta tcacaagtat agccaacttt ttcataaaat gcatgagcac
     2701 ccagacgatg attggcagaa tttaagcgga taaacccata accacatctt ttggcttctt
     2761 gttccaaccc ttgtagtaaa cttttaccga taccttgacc ttgcgcttga ggtgaaactg
     2821 ctaaagctaa gatattaaat cctgctttgg aatagagtga ttcgtaaact tcagcgtgga
     2881 catatccaag taagacatga ttaactgcat cctcatagcc aagtaggaaa tgatgggaat
     2941 cctgagacag tctagctagt tggctagccg tttcctctgg actaaaagta taacccaaag
     3001 cctcttggtt gatgtcacat atcgctttca catctgtttc ttgcaaatct cttagcattt
     3061 caaacctcct caaaagaaat ctctggcaac cgagcaagaa tatcttctcg tttaattgcg
     3121 ccttggcgta agattttcac cttgtctcca gacaaatcca caatagttga atcctgtcca
     3181 gttagaaaag catcgtcttc cagacccaga acctcttggt caaaatcctt cagaatttgt
     3241 tcaaaggtta caccacttgc ctgacctgag atattggcag acggcccaat caagggacct
     3301 gtctctcgaa ttaaatccag tgtgataggg tgactgggca tccgaaatcc aatagttgca
     3361 aggtcagaat ttacccaata gggaactcgg tcattggctt cgagaataat ggtcaaggga
     3421 cctggcaaaa aggtctctac aagtttttgt agataagctg gctgattctt agaaaagtgc
     3481 aagatatcct ctaaagaggc aacattgaga ttgagcgcct tgtctctagg acgacgtttg
     3541 agctggtaaa catggtcaac tgctttttcg tctaaggcct tggcaaaaag accataaacc
     3601 gtctctgtag gcagaatgac agctccaccc ttttccaact cttgtctaat cctgtccatc
     3661 atcaaccacg accatcctat cttgaccaaa ttggtccttg agtgttcgaa ctcttttttc
     3721 aggaagatgt ttcctaaaaa gttcgggaac actttgacct tgcttgtatc caatttcaag
     3781 gtaaatctta ccaccatcct tgagatagtc ttttgcatct tccgcaattc tacagtaaat
     3841 agctaggcca tcctcgtctg caaagagagc tagatgaggc tccgaatgca aaacattcaa
     3901 gcctacctct gactcatctt cacgagagat atagggtgga ttggatacaa ttatatcata
     3961 tttttcagaa atttctgcaa aacaatcaga ttttttaaaa aatatattaa gattttgatc
     4021 gctagcattc tctgatgcaa gctctaaggc ctcttgagaa acatctgctg ctgtcactga
     4081 ccaatctggt ctattttttg ctagagcgag agcaatagct ccactacctg ttccaatatc
     4141 cagaactgag agattcgtct caagattttc agttagaata agctccacta actcctctgt
     4201 ttctggacga ggaatcaaaa cccgttcatc cacctttaaa tgcattccat aaaaatctgc
     4261 ctgtccaatg atgtactgag ctggcttgtg agctgctagt tgctggtaaa tatcttctac
     4321 aaattgtttt tcttcctctg ttgtcacctc ctgctggagg gcaaaaataa agtctgtaaa
     4381 agatagattt ttcagactac gatagacaaa agagaggctt tccgcttcct ctccttgtct
     4441 tatcaactct tcttcaaaat ttgaaaataa ttgagctaat ttcattattt gtttaattct
     4501 tctagttttt gtgtttggtc ataaagcacc aaggcatcca caacttcgtc caatttacca
     4561 gacaaaatcg tatctagttt ttggagggtc aagccgatac ggtggtctgt gacacggttt
     4621 tgtgggaagt tataagttcg gatccgttct gaacggtcac cagtaccgat tgtcgactta
     4681 cgctcagcgt cttgttcatc ctgagcaatc tgagcaaagt ggtcagcgac gcgtgcacgg
     4741 ataatcttca tagccttctc gcggttcttc tgctgggtac gttcttcctg catctcaacc
     4801 ttgatattgg ttggcaagtg aacgatacga acggcagtcg caaccttatt gacgttctgt
     4861 ccaccagcac cagaggcgtg atagatgtcg acacgaaggt cttttggatc aatgtcgtat
     4921 tcaacctctt caacttctgg cataacaaga actgtcgctg tcgaagtatg aacacggcct
     4981 tggctttctg tcacaggaac acgttgcaca cggtgggcac ctgattcata cttaagctta
     5041 gagtatacag actgacctga aaccatagca accacttctt taaaaccacc gacaccattc
     5101 atagaggctt ccatgacttc aaagcgccaa ccttgggctt ccgcatactt ttggtacata
     5161 gttagcaaat ctccagcgaa aagtgccgct tcgtctccac cagctgctcc acggatttca
     5221 aggatgatat tcttgtcatc gtttggatcc tttggaagga gcaaaatttt cagtttttct
     5281 tcatattctt ctttttcagc cttggcatct ttgagttctt gcttggccat ttcttccaag
     5341 tccgcatctc cgcctgattc cttaatcatc tcttcggcat cgacgatatt ttgaaggact
     5401 tgtttatact cacggtaggc tattacggtg tcacgattgg aagcttcttc ttttgaaagc
     5461 tccataaaac gcttggtgtc tgaaacgaca tcagggtcac tcagcaattc tcctagttct
     5521 tcataacggt cttctacagc ttgtagttga tcatagatgt tcattttttc tccttatttc
     5581 tcaattgtta aatcatagat tgctactact tcattctcgg atatttcccc agtttcttta
     5641 aatccataac tgagataaca aaatcttgcc tgttcatttt ctggttcata agacaaccaa
     5701 agtttattgc ttaaacctgc tggcgctgtt cgaacatagt ctagtacttt atccataatt
     5761 ggtttaaaat atccttgatt ttgaaaattc ttatcaatca taaaacgaaa tagtaaataa
     5821 tttccactac taattccgat ctttttatca taagctatca tcacaaaacc tataattaca
     5881 tcattatcat aaactgccaa tggagctaca aaatctccat ttttagtgta gacgtatgct
     5941 tcagctaaac taattgcgtt ggttgcaatg aattgttttt gatattcctt gacatccaaa
     6001 tttaaaacat caaaataatt ttccattgta acatctctta gttcaattgt catagtttta
     6061 ctccttgtta gaggttatca ttggcgcaaa ataatgttta cggcaaaccg agatataggt
     6121 ttcattacca ccaatctgga tctgttctcc atcgtaaacg ggcagtccat cctgtgttcg
     6181 caacaccatg gtcgcctttt tcttgcaata ctgacagatg gtcttgattt cgtcaatctt
     6241 gtccgctaaa agcaagagat atttggaacc ttcgaacaat tcattgcgaa agtcattttt
     6301 caagccaaaa gccatgacgg gtatgtctaa ctcgtccaca acacgagcta ggtcgtaaac
     6361 atggtggcgt ttaagaaact gggcttcatc gaccaaaaca cagtaaggtt tttctggtag
     6421 gtctcggata tagccaaaga tatccgttgt ttcctcaatc gcaagggcag ggcgtttcat
     6481 gccaattcga ctcgacacat agccaacacc gtcacgcgta tccagagccg aggtcataat
     6541 gacaacacct tttccttgct cctcgtagtt ataggccact ttgagaatct caatcgtttt
     6601 accagagttc atggtcccat aacgatagta caactgtgcc atgtttcttg cttcacgtcc
     6661 atttctaaat ttttgctaca ttctagtata tcataatttt cttaagcttt aaacggcaaa
     6721 atgtggtaaa atggaagaaa tcaaaactag tggaggaagc tattatgcca tttgtacgca
     6781 tcgatttatt tgaaggacgc acgctcgagc aaaagaaagc tcttgctaag gaagtaacgg
     6841 aagcagttgt ccgcaacact ggagcccctc aatctgctgt ccatgtcatc atcaacgaca
     6901 tgccagaagg aacttacttc ccacaagggg aaatgcgtac taaataagct agcttaagca
     6961 gaattgctta ggctttttca atctccaagt agcattcatt gaagaaatat cctaaatttg
     7021 ttacaatttg aaaagaaact tggagaattt ccaagaaaag agctattaat taaaggaaac
     7081 attatgatta cacgtgaatt tgataccatc gctgctatct ctactccact aggtgaaggg
     7141 gctattggta ttgtccgcct gagcggaaca gacagttttg ctattgcgca aaagattttt
     7201 aaaggaaaag acttgaacaa ggttgccagc cacactctca actacggtca cattattgat
     7261 cctctgactg gtaaagtcat ggacgaggtt atggttgggg ctatgaagtc tccaaagacc
     7321 ttcactcgtg aggatattat cgagattaac acccacggtg ggattgcggt gaccaatgaa
     7381 attctccagc tagctattcg tgaaggggct cggttggcag aacctggtga atttaccaaa
     7441 cgtgcttttt taaacggtcg cgtagacttg acacaggcag aggctgtgat ggatatcatc
     7501 cgtgccaaga ctgacaaggc catgaacatt gcggtcaaac aattagacgg ctccctttct
     7561 gacctcatta acaatacccg tcaagaaatc ctcaatacac ttgcccaagt tgaggtcaat
     7621 atcgactatc ctgagtatga cgatgttgag gaagccacta ctgctgttgt ccgagagaag
     7681 acaatggagt ttgagcaatt actaaccaaa ctccttagga cagcacgtcg tggtaaaatc
     7741 cttcgtgaag gaatttcaac ggctatcatt ggacgtccca acgttgggaa atcaagcctt
     7801 ctcaacaacc tcttgcgtga ggacaaggct atcgtaacag atatcgctgg gacaacacga
     7861 gatgtcatcg aagagtacgt caacatcaat ggtgtacctc tcaaattgat tgatacagcc
     7921 ggtattcgtg aaacagatga catagtggaa caaatcggtg ttgagcgttc gaaaaaagcc
     7981 ctcaaggaag ccgacttggt tctactagtg ctaaatgcca gtgaaccact gactgcgcaa
     8041 gacagacaac ttcttgaaat tagccaagat accaatcgca ttattctact taataaaacc
     8101 gacctgccag aaacgattga aacttcgaaa ctacctgaag acgttatccg tatttcagtc
     8161 cttaaaaacc aaaacatcga caagattgaa gagcgaatca acaacctctt ctttgaaaat
     8221 gctggcttgg tcgagcaaga tgctacttac ttgtcaaacg cccgtcacat ttccctgatt
     8281 gaaaaagcag ttgaaagcct acaagccgtt aatcaaggtc ttgagctggg gatgccagtt
     8341 gatttgcttc aagttgactt gactcgtact tgggaaatcc tcggagaaat cactggggat
     8401 gctactccag atgaactcat cacccaactc tttagccaat tctgtttagg aaaataagaa
     8461 aaatccatga tccttcattc ggtcatggat tttattgtct ttattagtaa tctggtctta
     8521 agacacctgt tacagttgcc ttggtcgctt cgtagtcgcc atctacgaca accttgataa
     8581 tgcgtttagc atcttcttct ggtgctggaa caagaggtag acgagttggt ccagcttcaa
     8641 atcccatgta gttcaaaact gccttaactg gagcaggact tggataagag aagagagcat
     8701 taaccttagg aatgaattta cgttgaatag ctgcggcttt cttcatatca ctttctgcaa
     8761 tggcaataaa catctcgtgc atttcatccc catttgtatg agaggcaaca gaaataaccc
     8821 catccgcccc aaggttcatg gcatggaaag catctccatc ctcaccagta tagaccaaga
     8881 actcttctgg tttgtgctca atcaagtaag ccatattagc caagctagta cattctttga
     8941 caccgataat atttggatga tcagccaagc gaagcatggt ttctggagtt aattcaacaa
     9001 ccacacgccc tggaatgtta tagataataa ttggtaggtc agaagcatct gcaatggctt
     9061 taaaatgctg atacattcct tcttgagaag gtttgttgta gtaaggaaca atagcaagac
     9121 cagcagcaaa accgccgaat tccgctactt ctttgacaaa ctcgatagag tcacgcgtat
     9181 cattagtacc tacacccgca atcaaaggaa ctcgtccatt gacaatcttt tgtacagccg
     9241 caaataactc caactcctca tcgtgggtca aagttggact ctcagcagtg gtccctgcga
     9301 gaagaattcc atccgtatga tggtccaata aatgctcaat caaggctgga atagcatcaa
     9361 agttgatgga accatcctcg tggaagggag taataaaggc tgtgatgatt ttacactctt
     9421 ttaaatcttg ataagacatg aaaacacctc tctatttcaa agaaggagtt catctgaact
     9481 cctaaacgat atgactattt taattcaaat ttcaattcag ctgttggacg aaccaatcca
     9541 cgttcatgaa gagtttcagc aatctgaact gagttccaag cagcaccttt gagaaggtta
     9601 tctgaaacaa cccacatgtg aattcctttt tctgcatcca agtctttacg gatacgacca
     9661 acaaaggtat cacgcgaacc aactgcattg atagcttgag gatagatttg atgagctaca
     9721 tcatcttcaa gaacagcacc tgggaaggct gcgatagctg cttttacttc ttcgattgga
     9781 gccacttctt ttgtttcgat ataaacagac tcagagtgag ctgacaagac tggaatacgc
     9841 acacatgttg cagatactgc aatgctatca tcttccataa ttttcttagt ttccttggtc
     9901 atcttcatct cttcgtaagt gtaatcattg tcagtgaaga catcgatttg tggaagagca
     9961 ttgaaggcga taggataatg tttcttgtca ccgcctgaag gtaagatttc cgcatgcaaa
    10021 tcacgtggtt tcgctccatc attcaagacc tcacgaagtt cacgttgcgt ctcaagaatt
    10081 gctcccatac cagcacctga aacggcttga taagttgaaa caatgatacg gtccaagccc
    10141 catttttggc gaactggctc aagagctacc atcatttgga ttgttgaaca gttagggcaa
    10201 gcaataatcc cgttgtgggc atcaagtgcg tgagcattga cctctggaac aaccaaagga
    10261 acgtctggat tttgacggaa gtaagatgta ttatctacta ctactgcgcc agctttaact
    10321 gcgtatggtg catacttagc tgatgtcgaa ccacctgctg aaaaaagagc aatatcaact
    10381 ccttcaaaag ctgtttcagt cgtttcttca atcgtaatat cttgatcttt aaatttcaat
    10441 gtcttgcccg ctgaacgtgc agaagcaagg taacggattt tatcgattgg aagtgttgat
    10501 tcttccaaca tttttatcat ctgagcaccg acagcacctg tcgcgccgac tacagcaact
    10561 gtatatccca taaataacct ctttcggaat tttctaaaaa tttctataat agaagtatta
    10621 tactactttt tccatgaatt gcaaagcttt ttcatcattt tttggaaaat aaaaatccca
    10681 gtcgctagac tagggactaa gaggcatctt catgtgatga gcaggttcac acaactcatc
    10741 aaggtccgct cctgcgttat gacctcctta tgctcaatag tagtccgaag actacctatc
    10801 gactcatcgc atattctata ttactagaga tacttgattt tgtcaataag aaattttttc
    10861 gatcgagtat aacaaaaaac tctcgtattc aagagtttcc ctatacatat catgctaatt
    10921 gccgggattg aaccggcgac ctcatcctta ccatggatgc gctctgccaa ctgagctaaa
    10981 tcagcttact taagaagtat actatattca gaaaaacttg tcaagtttcc ttagaaattt
    11041 tccataggaa aaagccaggt aagaactacc tagcttatct tcttctattt acttaaatca
    11101 attcgacgac caattttacc aataataatc gctccaataa tcaatgaagc aaattcaccg
    11161 attcctgttg taaaccaagt aaggaagaat ggtaattctg ctacaatatt taactctgca
    11221 gcgatagtaa acatggaaat tgaaaagagg attgaaaaga agaaatgatc ttttcgaatc
    11281 aatccgttaa agaggtaatc cttgctatat tttgcaaaaa gccaaacacc taaactgagg
    11341 aatactaagg ttgaaccacc accaacaaag acatctagca gtccgaagct aaagaaatta
    11401 gcaatcatac aaccgatggt aactccgatg atgtacttag gattgtaaaa agccataaag
    11461 ttcatcattt ctgaaatacg aaactgataa gcaccatagc tgatggcatt taggggcggt
    11521 gtgacagtca aaacgacgta gatagcagcg acgattgcaa tatctgcaac atcacgaata
    11581 gttaattttt tcatcttttc tcctttggcg gttatccgcg tgtaatatgc ttggtgaaag
    11641 aagctaagca ccaagggttg attcattcaa ccttaccagt atagcacaat agcctgcttt
    11701 atgctatact taaagcatga aaaaacctat tcaaaaattt tttaacaatg aaatcttagc
    11761 ctatctcttt tttggccttg cgacaactct agtttcgatt ctctcacgac tagtcattta
    11821 tcaactaagt catcaggaac tccttgctac ggccttggca aatattatcg gtatcttatt
    11881 tgcctttatc acaaatgata cgattgtatt taagcaaaaa aagaaaaata gacttatccg
    11941 tttagtgaaa ttttctcttg cccgcctttc tacttttctg ttagacttgt ttttaacttt
    12001 tctctttgta acccaatttc ctcacatcat agggcaattc gtaactgaaa acattgataa
    12061 gataaatgct attgaaacaa taattgcaca aatattcatt attatcatca attatatctt
    12121 gagcaaggtc tatattttta gaaaataatg ctccatttat gagcctttta attgccttct
    12181 ataatgattt aggatacaat gaaacacgaa acattgaaag gaaaacagta tgttaaaaaa
    12241 tctaaaatcg ttcttgcttc gaggaaatgt tattgacctt gctgtcggtg ttgtcattgc
    12301 ctctgctttt ggtgctatcg ttacttcact tgtaaacgac attatcactc ctcttatttt
    12361 aaatcccgct ttgaaagctg ctaaagtcga acgtatcgct caactttctt ggcatggagt
    12421 cggctatggt aactttttaa gtgctattat caattttatc tttgtgggta ccgccctctt
    12481 ctttattatc aagggcattg aaaaagcaca gaaactgact ggcataaagg aagaaaaaac
    12541 tgccgaaaaa aaaccaaccg aattggaagt ccttcaagaa ataaaagctc tccttgagaa
    12601 aaaataatcg attaaaagga tctagcgcaa agctaaatcc ttttttcttt actctttggg
    12661 taacttgcct gctttttcaa gcattttctt gatcagataa ggcatcttaa cattttcacg
    12721 accttttttc tcaatcaatt ttttcacaaa ttctggcatt tgtaaatctt gagattcggc
    12781 taagatattt ttagtctcat ctgaaggcac taactcatca aaggtttctt cttctgggag
    12841 aaattcctta aactcttgat tttcattgac tctaactgta ttgaccaagg catcaatcaa
    12901 acgagaatct gtatttggca ttggtggacg atggtagttt accccaaatt cctggcataa
    12961 gtcataacat tccacatcat tgtcaaacaa gacctcaata tgctcactga tgaaactaat
    13021 aggtacaaaa atataatggt ctggatgttc tgtctgttct ctgagatact ccaaaacatc
    13081 tggtttaatc catggaatac caatatcact ctcactctgc caagtgttgg tatattgttc
    13141 tgaactcaag cccagttttt cagcgactaa cttgctattt tcgaaaattt ggtctatata
    13201 aggatcacca aagtccaagg caaaaatagg cacactatgg gctgaaaaga tgactttaaa
    13261 gctgtcttgc tttacttctt cttttaaaat cttagcaatt tcatctgccc aatagtttaa
    13321 gagcgcttct tcctgatacc agtccttaat gaccaaaaat tgaatttgtt tgctttctaa
    13381 aaatttttca tagcccatga cagagtagaa agaataatgg ggctccaaaa tcaaacaaat
    13441 acactgttca attccgtctg cttccatttg accgatcaca tctggaataa agggactgga
    13501 aaacttatta gcaaagtaga cactgtattc attccccagc ctagcttcta ccaaggcaac
    13561 ctcttgacgg gtaatttttt gcagaggcgt gcctccaatt cgtacataat tatcatagag
    13621 agtctgaatc tcgtggtctt ggggtctcac cccacgacga atatttgtga aaaaatcagc
    13681 tacaccttca aaggtaatct cttctggtga accgaaggtc atcattaaaa ttgctttttt
    13741 catgactgct tccttgtgat gtttttatca agtttatttt atcattaatt catcgataag
    13801 taaacgctag catagggaca ttctcaatct tctgtctttt gtttttctct tctttctatg
    13861 atacaatgga aaaaataaat tcaaaaggag tttttttatg acttatccaa atctcttgga
    13921 ccgcttctta acctacgtta aggtcaacac gcgctctgat gaacactcta ctactactcc
    13981 aagtacacag agtcaggttg acttcgcaac aaatgtccta attcctgaaa tgaaacgtgt
    14041 tggactgcaa aatgtttact atctaccgaa tggttttgct attggaacct tgccagccaa
    14101 cgatccgtct ttaacacgta agattggttt tatatcgcac atggatactg ctgattttaa
    14161 tgctgaagga gtcaatccac aggtaattga aaactacgat ggtggtgtga ttgaactagg
    14221 gaattctggt ttcaaactcg atccagctga cttcaagagt cttgaaaaat atccaggaca
    14281 aacgctcatc acaacagatg gaacaacctt gctaggtgct gatgacaagt caggaattgc
    14341 tgaaattatg acagccattg aatatctaac tgctcatcct gaaattaagc actgtgagat
    14401 tcgtgttggt tttggtccag atgaagaaat cggtgttggt gccaataaat ttgatgcaga
    14461 agattttgat gtggattttg cctacactgt tgatggtggt ccactaggtg aacttcagta
    14521 cgagactttc tcagccgctg gtgctgaatt gcatttccaa ggccgtaatg tccaccctgg
    14581 tactgccaaa gggcagatgg tcaatgccct tcagctagca attgattttc ataatcaact
    14641 tccagaaaat gaccgacctg agttaactga aggttaccaa ggtttttacc atctaatgga
    14701 tgtgacaggt agtgttgagg aggcgcgtgc aagctacatc attcgtgatt ttgaaaaaga
    14761 tgcctttgaa gcgcgtaaag catccatgca atctatcgct gataagatga atgaagaact
    14821 tgggagcgac cgtgtcactc tcaacttgac agaccagtac tacaatatga aagaagtcat
    14881 tgaaaaagat atgactccaa ttaccattgc taaagccgtt atggaagatc taggtatcac
    14941 gcctattatc gaaccaatcc ggggtggaac agacggctct aagatttcct ttatgggaat
    15001 cccaactccg aatatctttg caggtggcga aaatatgcac ggacgttttg aatacgttag
    15061 ccttcagact atggaacgtg cagttgatac catcattggc atcgtagctt ataaaggcta
    15121 aaaagacgag gtggctcagc tacttcgcct ttctttttat tctactggtt tttcttgatt
    15181 tccagtagtt gtagaagatt ctgttgtttc attttctgaa gttgattcag caggtttaga
    15241 atctcttgta ttgcttggtt tgttttcgtc gctagcagtt tcaatgttag attctgcagt
    15301 tgcgtttggt tggttctcag cactggtgtt atcaccattt gcttcagcat ttcttgctgg
    15361 acttgtttct tcacttgcgc tagcttttga ctggatttga tgattcaaaa ctagaatagc
    15421 ttttgtcggt tcaagtaaag ctgttttgtc tttactctta gcagaaagtt gatctaataa
    15481 tgcatccacc ttatcaaagt ccgcatcaga tccattatta ctttctaaat aagagtgaag
    15541 cgacatgaga atatcgtaga gtttttgata gagtacaagt gtctgaggat cttgctcagc
    15601 attttccttt tcttgttgaa gggcgctagc gatacgagtc aaaacatctt ttacctgact
    15661 gtttacttca tccaagtctg catcagcctt gtttgtggca gcttttagat tttctacttc
    15721 ttctgccaag gattgtctga ttccttcttc atggatttgt tccaagagtt gatttgcctt
    15781 gctcaaaaga ctttctactt cttccttgct atctgtcgca gattattggt tgccatctac
    15841 catgtactcc taaaacagga gagttataat ccaagattac aaggccttac agaaataaga
    15901 aatccagata agacaatgtt cgtccaagac gctattcgct tcgcacagca gcacggattc
    15961 aatatgcttt aattttaaag tttaggtgtc aagacctctt tttagtgtgc ccaaaattta
    16021 gagaagtaat caatcaacta acttttattt ttttcaaact ttcagtaaac tgacctaaag
    16081 ctaactcaat ctgtctttgt tcgataggct tgtctttgta gatgcttctg ctatcagcta
    16141 gaagttgatc tacttttgcc aagactgcct tctcatcaaa agttccaggt tgatagttgg
    16201 attgcaggga tggaatcttg tttttcaaag ccgcttcata tcccttagtt tgaaccttga
    16261 tgtagtgatt gtggtcgcca cgaggaatca caaaaccttc tgaatcttca cttataattc
    16321 gattggcatc aaaaccatga ccatcttctt cctcatggtg gacatgtagt gacggattac
    16381 ttaatacaga actagaagaa cttcctacct tttccgtgtt agagtgtgat gggggattgt
    16441 taagagatga cttaggaata tagtgatagt gaccccatgt cttactatat aagcatcacc
    16501 tgtatctctg acaatatcat tagggttaaa gacataacca tcatctgctg cagaaacacc
    16561 attattcggt gtcaccgaca aagattgact gagagctgta gtattctctg ataattatac
    16621 ttttgcagct gctaattcac ctgccgacaa gtcactctca ggaatgaaat gatagtgacc
    16681 accatgtggt actatagtag attgaaatag aatatgagca aattgataag gggattttaa
    16741 agtaatttct aacaatgatt tagaaactat gatgtgctat tctaaattca actcactata
    16801 tataaccatc atcggtagta taacgtccct gtaattttgc tacagatact tctgcactag
    16861 ctcctttatc gtctttacca tgttcttgtt tttggcgatt gatttcatct tttattcgta
    16921 cattttctgc atgagcttga tctttaaggt aaacataata ctttccatct accttaataa
    16981 tatatcctcc cttaacctaa ctgacgatat cttgatcttt cggctgatag ttgggggctt
    17041 tcattaatag ctcttcacta aagagcgcat ccaaaggaac tttaccatta tagtagtgat
    17101 aatgatcgcc atgagaagtt acataacctt gacctgtaat cttaataaca atttgttttg
    17161 cttgaattcc ttctttttga ctaacctagt ctggagtcaa attttcagtc ttcttagtgt
    17221 ctttattact gtttacatat gaaacacgat ttttatctgt attagcctgt tagctatgtt
    17281 ggttcagagc ataaacacac agacttaagg aaaggataac aacagatcca gctactatat
    17341 atttcttttt aaatttcata attacctcat ttctat
//
LOCUS       DAVMHG010000034        17144 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMHG010000034 DAVMHG010000000
VERSION     DAVMHG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.034"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..316
                     /locus_tag="VYR08_000885"
     CDS             <1..316
                     /locus_tag="VYR08_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466439.1"
                     /translation="FWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAP
                     SIVRWIGQLNPLTYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILI
                     ER"
     gene            474..947
                     /gene="tnpA"
                     /locus_tag="VYR08_000886"
     CDS             474..947
                     /gene="tnpA"
                     /locus_tag="VYR08_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEV3466440.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            1204..1425
                     /locus_tag="VYR08_000887"
     CDS             1204..1425
                     /locus_tag="VYR08_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3466441.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            1872..2174
                     /locus_tag="VYR08_000888"
                     /pseudo
     CDS             1872..2174
                     /locus_tag="VYR08_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2330..3559
                     /locus_tag="VYR08_000889"
     CDS             2330..3559
                     /locus_tag="VYR08_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV3466442.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3579..6275
                     /gene="ppc"
                     /locus_tag="VYR08_000890"
     CDS             3579..6275
                     /gene="ppc"
                     /locus_tag="VYR08_000890"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV3466443.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6358..6575
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6688..7692
                     /locus_tag="VYR08_000891"
     CDS             6688..7692
                     /locus_tag="VYR08_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3466444.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7761..8627
                     /locus_tag="VYR08_000892"
     CDS             7761..8627
                     /locus_tag="VYR08_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466445.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8624..9382
                     /locus_tag="VYR08_000893"
     CDS             8624..9382
                     /locus_tag="VYR08_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466446.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIIMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9472..11232
                     /gene="dnaG"
                     /locus_tag="VYR08_000894"
     CDS             9472..11232
                     /gene="dnaG"
                     /locus_tag="VYR08_000894"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV3466447.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDDNQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            11235..12344
                     /gene="rpoD"
                     /locus_tag="VYR08_000895"
     CDS             11235..12344
                     /gene="rpoD"
                     /locus_tag="VYR08_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV3466448.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12358..12687
                     /locus_tag="VYR08_000896"
     CDS             12358..12687
                     /locus_tag="VYR08_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3466449.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12802..13845
                     /gene="cpoA"
                     /locus_tag="VYR08_000897"
     CDS             12802..13845
                     /gene="cpoA"
                     /locus_tag="VYR08_000897"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV3466450.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13861..15186
                     /locus_tag="VYR08_000898"
     CDS             13861..15186
                     /locus_tag="VYR08_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV3466451.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            15217..15393
                     /locus_tag="VYR08_000899"
     CDS             15217..15393
                     /locus_tag="VYR08_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3466452.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15452..15586
                     /locus_tag="VYR08_000900"
     CDS             15452..15586
                     /locus_tag="VYR08_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV3466453.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15650..16960
                     /gene="obgE"
                     /locus_tag="VYR08_000901"
     CDS             15650..16960
                     /gene="obgE"
                     /locus_tag="VYR08_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV3466454.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16970..17128
                     /locus_tag="VYR08_000902"
     CDS             16970..17128
                     /locus_tag="VYR08_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466455.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5260 a         2996 c         3803 g         5085 t
ORIGIN      
        1 attttgggac tcccttgcaa ttttacttac aatgtttatc aatgattaca gaagacgtga
       61 tattgtaata cgttttgtac taacaccgct tggttttaca gctcctgttt tctacttaat
      121 agattctgct cctagtattg tgagatggat tggtcagtta aatcccttaa cttatcaatt
      181 aactattttg agaaactttt attttaaaaa ttcaacaact ttggaattag ttttcttatt
      241 gttaacatca ttacttgtcc ttatatctgt atcttttatt ataccaaaga taaaattgat
      301 actgatagaa agataaaagt tgggtcatcc aacttttttg ttgtctcccg aaaaccacta
      361 gctatgctag tggttccata gagcttttag cgtggtaaca aaaagaacct cctaaaatga
      421 taagatagaa gtggtttctc cgccactaca acatatcata caggaggtac ctcatgagag
      481 aggataatca aagtttatca cataccacat ggaattgtaa atatcatatt gtttttgcac
      541 ccaaatatcg tcgtcaaatc atttatggca gatacaaagc tagtatcgga agaatcatac
      601 gtgacttatg tgagcgtaag ggtgtaataa tccatgaagc gaatgcttgt tcagaccata
      661 ttcacatgct tatcagtatt cctccgaaac ttagtgtttc gtcctttatg ggctatttaa
      721 agggcaagag cagtttgatg atttttgata agcatgcgaa tttaaaatac aaatatggca
      781 atcgcaagtt ttggtgtaga ggctattatg tagatacggt aggccgtaat cagaaagtga
      841 tagctgaata tattcagaat caattacaag aagacagagt agcagaccag ctcacgttat
      901 tcgagtcagt agatccgttt actggcgaaa taaataagag gaagtaacta aggtgcttta
      961 gcacctgctc gggaaagtgg tgcgcgagga agctatttcg gtgggccttt ggccctggcc
     1021 ggtagaagcg gcttatagcc gcagaacaaa ccaccagttc acactggtgg ttttgattta
     1081 aaaaacttga tacataaaaa taaaagtcta tataaaggat ggtaaaattc ctgttgtccg
     1141 atttggacaa tatcctaaat agttacaata tatggtctat actttttctt aggagaaagc
     1201 tagatgtaca gacgtttgag agatttgagg gaggatcatg atctgcccca aaagcaaata
     1261 gctacaatac tttcgtttac aaattcagct tatgccaaaa ttgaacgggg tgagcatgcg
     1321 ttgacggctg atgtattggt taaactctca gatttctatg acgtcagtac agactattta
     1381 ttgggattaa ctgattttcc tgataaaatt cgctttagaa aataatctcc tcaatttcat
     1441 agagtttgaa aatgagtgag attttttatt tgccctttga caactgaata gcctaaaatg
     1501 gtactttcct catttgtgga gcaaatttga atggctcgcc atgataagag cgattttaaa
     1561 accatcaata aaatagagcg atactttata tgccatgata caaatgatat acaatgatac
     1621 ttctgaccgt tcagcctgcc aacgtaaaag agcagcaagt gaaattctta tgatgacttc
     1681 atcagtcatg ccacgttgaa tgtgtgagtt tgttagataa acgcaattaa tcctcaaaag
     1741 gttccccgaa ccttttgagt tctacagacg catcacgtgg agtgtgtaag cttgttgcta
     1801 aaagcgtaaa aaccttggaa cgaaaggaat aatagacttt ctgcgaaaca aaaatataat
     1861 acaataaaac tatgaatgat gaagcaagta aacaattgag cgatagccgt ttcaagatcc
     1921 ttgtaggtgt tcagcgcacg acttttgaag agatgttagc tgtgttaaaa acagcttatc
     1981 aacgtaaacg cgcaaaaggt ggacgaaaaa gcaaattaag cctagacgat ctccttatgg
     2041 taactattca atacatgcga gaatagagca cttatgaaca aattgcggct gattttggca
     2101 ttcacgaaag caacttaatc cgtcggagtc aatgggttga agcaactctt attcaaaatg
     2161 gttttacgat ttcaaattct gccttaattc tgtaaaaaca gtaaaattcg aaggattgta
     2221 aggtaagagt ttttttcttt ctgaaaaaat ggtataatag caatcaaaac tagaaaataa
     2281 aacggaattt ggaacagatt tgtctgtatc ctagtagagt ggtgatacta tgaagattag
     2341 taagaggcac ttattaaatt attccatctt gattccctac ttgcttttat ctattttggg
     2401 cttgattgtg gtctattcga ccaccagtgc tattttaatt gaagaaggca agagcgcctt
     2461 gcagttggtt cgaaaccaag gaatcttttg gattgttagt ttgatactga ttgccttaat
     2521 ttataaattg agactagatt ttttgagaaa tgagcgacta atcattttag ttatattaat
     2581 agaaatgctt ttattgttct tggctcgttt tattggtatt tccgtaaacg gggcatacgg
     2641 ttggatttcg gttgcaggaa taactattca gccagctgag tacttaaaaa tcattattat
     2701 ttggtattta gctcaccgat tctccaaaca gcaagaagaa atagctactt atgattttca
     2761 agttttgact caaaatcaat ggcttccccg tgcttttaat gattggcgat tcgttctcct
     2821 agttctgatt ggaagtttgg gaattttccc tgatttagga aatgcgacta ttttagtctt
     2881 ggtttccttg attatgtata cagttagtgg aatcgcttat cgctggtttt caaccattct
     2941 ggcgctcgta tctgccgctt ctgtctttgt cttgaccact atcagcctaa tcggtgttga
     3001 gaccttttca aaaattccag tattcggcta tgtagccaag cgctttagtg ccttttttaa
     3061 tccttttgcc gatcgtgctg atgcaggtca ccagttagct aattcttatt ttgccatggt
     3121 caatggcggt tggtttggtc taggtcttgg aaactcgatt gaaaaacgag gttatttgcc
     3181 agaagctcat acagactttg tcttttctat cgtgattgaa gaatttggct ttgttggtgc
     3241 cagtcttatt ttagctctct tgtttttcat gattttgcgg attatcttgg tcggtatccg
     3301 agcggagaat cctttcaatg ccatggttgc actcggtgtc ggagggatga tgttggttca
     3361 ggtatttgtc aatatcggag ggatttcggg cttgattcca tctacaggag tgactttccc
     3421 cttcttatcc cagggtggaa atagtcttct agtcttatca gtggcagtag cctttgtctt
     3481 aaatattgat gccagtgaaa aacgcgctaa attgtaccga gaattggaaa atcaaccaat
     3541 gaaccttctg ttgaagtagg ataaagaaag gatagtttat gtctcttcaa aaattagaaa
     3601 attatagtaa taaaagtgtt gtgcaagaag aagtcttgat tctaacagaa ttactggaag
     3661 atattactaa aaatatgctt gccccagaga cctttgaaaa aataatacag ttgaaagaat
     3721 tatcaacgca ggaagattat caaggtctaa accgtctagt gactagctta tcaaatgatg
     3781 aaatggtcta tatttcacgc tatttctcta tcttgcctct tttgattaat atttcagagg
     3841 atgtggattt agcttatgaa atcaatcatc aaaataatat tgatcaggac tatttaggta
     3901 aattatctac aacgattaaa ttggtagcag aaaaggaaaa tgccgttgag atcctagaac
     3961 acttgaatgt tgtccctgtt ttgacagccc atccaacaca agtgcaacgc aaaagtatgt
     4021 tggatttaac aaatcatatt catagtcttt tgcgtaaata ccgtgatgtt aagttggggt
     4081 tgatcaataa agataaatgg tacaatgatt tgcgtcgtta catcgaaatt atcatgcaga
     4141 cagacatgat tcgtgagaaa aaattaaaag tgactaacga aatcacgaat gctatggaat
     4201 attataacag ctcctttttg aaagctgtac ctcatttgac gacggagtat aagcgcttag
     4261 cgcaagcgca tggtctgaat ttaaaacagg ctaaaccaat caccatgggt atgtggatag
     4321 gtggtgaccg tgatggaaat ccatttgtta cagcaaagac cttgaagcag tctgcactca
     4381 ctcagtgtga agtcatcatg aactactatg ataaaaagat ttaccaactt tatcgtgaat
     4441 tttctctttc aactagcatt gtcaacgtca gcaagcaagt cagagaaatg gctcgtcaat
     4501 ccaaggataa ctcgatttac cgcgaaaaag agctttaccg tcgtgccttg tttgatattc
     4561 aatcaaaaat tcaggcaact aaaacctatc tgattgagga tgaagaagtt gggactcgtt
     4621 atgaaaccgc caatgatttc tacaaggatt tgattgccat tcgagattct ctactagaaa
     4681 ataagggcga gtccttgatt tcaggtgatt ttgtggaatt attgcaggca gtagagatat
     4741 ttggttttta cttagcatca attgatatgc gacaagactc tagcgtctat gaagcctgtg
     4801 tggcagaact cttgaaatca gcaggaattc attctcgtta tagcgagttg agcgaagaag
     4861 aaaagtgtga ccttctcttg aaagaattag aagaagatcc ccgaattctt tctgcgactc
     4921 acgcagaaaa atcagaatta ttagcaaaag aattagctat ttttaagacg gctcgtgttt
     4981 tgaaagataa gttgggagat gatgtcatcc gtcagaccat catttcacat gcaaccagcc
     5041 tttctgatat gctagaatta gctattctgt taaaagaagt aggactggtg gatacggaaa
     5101 gggcgcgtgt tcagattgtt cccctttttg aaacaattga agacttggat cattcagagg
     5161 aaacaatgag aaaatatctt tctcttagcc ttgccaaaaa atggattgac tcacgaaata
     5221 actaccaaga aatcatgctt ggctactctg acagtaataa agatggcggt tacttgtcat
     5281 catgttggac cctctacaag gctcaacaac aattgactgc tattggagat gaatttggcg
     5341 ttaaggttac cttcttccat ggtcgtggtg gtactgtcgg tcgtggtggt gggccaacct
     5401 atgaagccat tacatctcaa ccgctcaagt ctatcaagga tcgtatccgc ttgacggagc
     5461 agggtgaagt aattgggaat aaatacggta acaaagacgc cgcttactat aaccttgaaa
     5521 tgctagtatc ggcagctatt aaccgtatga ttactcagaa gaagagcgat accaataccc
     5581 caaatcgtta tgaaaccatt atggatcaag tagtggaccg tagttacgat atctaccgtg
     5641 atttggtctt tggtaatgag catttctatg attatttctt cgagtcaagt ccaatcaagg
     5701 ctatttcaag ttttaatatt ggttctcgtc cagccgctcg taagactatt actgaaatcg
     5761 gtggtttgcg tgccatccct tgggtattct catggtcaca gagtcgtgtt atgttccctg
     5821 gatggtacgg ggttggttca agcttcaagg aatttatcaa taaaaatcca gagaatattg
     5881 ctatcttacg agatatgtac caaaattggc ctttcttcca atcgcttctt tcaaatgttg
     5941 atatggtttt gtcaaaatca aatatgaata ttgcttttga atatgctaaa ctttgtgaag
     6001 acgagcaagt taaggccatc tatgagacta ttttaaatga atggcaagtt actaagaacg
     6061 ttatcttggc tattgaagga catgacgaac tcttagctga caatccatat ctaaaagcta
     6121 gtctggatta ccgtatgcct tactttaata ttctcaacta tattcagttg gagttgatta
     6181 aacgccaacg tcgtggagaa ttgtccagtg atcaagaacg attgattcat atcaccatca
     6241 acggaattgc gacaggattg cgtaattcag gttgataatt ttcaagagtg aatgctaaaa
     6301 gtgaatatca aaaaaattct aatagactat tgacaagtag tttaaaaatg atataattta
     6361 accattcaga aaagtaatca tacaaacttt ttagagagtc tgtggtagct gaaaacagat
     6421 aagtggcaat gatgaaaatt gggctgaatg ctatttagaa tttgaaatta taaaaattcg
     6481 gtaagcacac cttacagtgc atctcgttat tgcgagactg agcgataggg aaattcccta
     6541 taattgaggt ggtaccgcgc atcgacgtcc tcacacaagt tttttgtgtg aggatttttt
     6601 tgatggaggt tagtatggaa agaaaacgat ggcgtcgctt gtttagataa gtgaaatatg
     6661 ttaaaggaaa taaaaaggag aaacagaatg aaaaataaac gtttaattgg aattattgct
     6721 gcattagcag tcttagtagc aggaagcttg atttattctt caatgaataa atcagaagct
     6781 cagaataata aggatgagaa gaaaataacc aagattggtg tgcttcaatt tgtgagccat
     6841 ccatcccttg atttgattta taaagggatc caagatggac ttgcagaaga aggatataaa
     6901 gatgatcaag ttaaaattga ttttatgaac tcagaaggtg accaaagtaa ggttgcgaca
     6961 atgagtaaac aattggttgc aaatgggaat gaccttgtgg ttggtatcgc aacaccagca
     7021 gcccaagggt tggctagtgc aacaaaagac ctaccggtta tcatggccgc tattacagac
     7081 ccaattggtg ctaacttggt taaagatttg aaaaaaccag gtggcaacgt tacaggggta
     7141 tctgaccaca atccagctca acaacaagtt gaactcatca aggctctgac accgaatgtg
     7201 aaaacaatcg gagctcttta ctcaagtagc gaagacaatt caaaaacaca ggtcgaagaa
     7261 tttaaggctt atgctgaaaa agcaggtctg acagtggaaa catttgcagt tccttcaaca
     7321 aatgaaattg cctcaactgt cactgttatg actagcaagg tagatgctat ttgggttcca
     7381 attgataaca ccattgcatc aggatttcca acggttgtct ctagcaatca aagttctaag
     7441 aaaccaattt atcccagtgc gacagctatg gtagaagtag gtggtttggc atcagttgta
     7501 attgaccaac atgaccttgg tgtggcaaca ggtaaaatga ttgtgcaagt cttgaaaggt
     7561 gcaaaaccag ccgatacccc agtcaatgtc ttttcaactg gtaagtcagt catcaataaa
     7621 aaaatagcac aagaactagg tattactatt cctgagtctg ttctcaaaga agcaggacaa
     7681 gtcatcgaat aaataaggta gggaggagga gactcttccc attttttaaa aagatgaata
     7741 tagaaaggat taaataaaat atgattgttt ccattatttc tcaaggattt gtctgggcta
     7801 ttctaggtct gggaatcttt atgacattta ggattttaaa ctttccagat atgacgacag
     7861 aaggttcctt ccctcttggg ggagctgttg ctgtcacttt gataaccaaa ggcgtgaacc
     7921 catttttagc gacacttgtt gctgtaggag caggttgttt ggctggaatg gcagcaggcc
     7981 ttctttatac aaaagggaag atcccaacct tgctctcagg gattttggtg atgacttctt
     8041 gtcactcaat catgctcttg attatgggac gtgcgaattt aggcctgctt ggaaccaagc
     8101 aaattcagga tgttttgcct tttgattcgg atttgaatca actcttgaca ggtctcatct
     8161 ttgtgagtat tgttattgct ctcatgctct ttttcttgga cactaaactc ggacaagcct
     8221 atattgctac aggggataat cctgatatgg ctagaagttt cgggattcat actggacgca
     8281 tggagctcat gggcttggtc ttatcaaatg gtgtgattgc ccttgcaggt gccctcattg
     8341 ctcagcaaga aggttatgcc gatgtgtctc gagggatcgg ggttatcgtt gtggggcttg
     8401 caagtttgat tattggagaa gttattttca agagtttgag cttggcagag cgtttggtta
     8461 ctatcgttgt aggttctatc gcttatcaat ttttagtgtg ggcagttatc gcacttggct
     8521 ttaatacaag ttaccttcgt ttatacagtg ccttgatttt agcagtctgc ctcatgattc
     8581 caacatttaa gcaaacaatc ttgaaaggag ccaagttaag caaatgacag caattgtaga
     8641 attaaaaaat gcaaccaaaa tcgttaaaaa tggctttgat gaagaaaaga ttattttaaa
     8701 tgatgtttcc ttagaaattt ttgaacggga ctttatcacg attttgggcg gaaatggtgc
     8761 tggaaaatca actctcttta acactatagc agggacctta tcactaacta gtggaactat
     8821 ccgtatttta ggtgaagatc tcactaagtt ttcacccgag aagcgtgcca agtacctgtc
     8881 tcgtgtcttc caagatccaa agatggggac agctccccgt atgacggtcg ctgaaaatct
     8941 tttaatcgcc aagtttcgtg gtgaaaagcg tggattgtta ccacgacgct tgactagcta
     9001 taaggatgaa tttcaggcaa ccattgaaaa agtaggaaat ggtcttgaga aacacttgaa
     9061 tacaccgatt gagttcttat caggtggaca aagacaggct ttgagtctct tgatggcaac
     9121 cttgaagcga cctgaattac tcctgttaga tgagcatact gctgccctgg atccaaagac
     9181 tagtgttgct ttgatggaat tgacagatga atttgttaag aaagatcagc taacagccct
     9241 tatgattact catcatatgg aagatgctct caaatacggc aatcgcttga ttatcatgaa
     9301 agaaggacgg attatccaag atttaaacca agaagaaaaa gcaaaaatga aaatctctga
     9361 ttattatcaa ctctttgaat aaagtaggga aaatgagtga aatggtttac tttttttctg
     9421 aaataaagta tactatataa agtaaactat gataacatgg aggtattgtg tatggttgac
     9481 aaacaagtca ttgaagaaat caaaaacaat gccaacattg tggaagtcat aggagatgtg
     9541 atttctttac aaaaggcagg acggaactat ctagggctct gtccttttca tggtgaaaaa
     9601 acaccttctt tcaacgttgt agaggataag cagttttatc actgtttcgg ttgtggtcgc
     9661 tcaggtgatg tctttaagtt catcgaggag taccaagggg ttccctttat agaggcagtc
     9721 caaatcttag gtcagcgtgt tggaattgag gttgaaaaac cgctttatag tgagcagaag
     9781 ccagcctcac ctcaccaagc tctttatgat atgcacgaag atgcagccaa attttaccat
     9841 gcgattctca tgacaacgac tatgggcgaa gaggccagaa attaccttta tcagcggggt
     9901 ttgacagatg aagtgcttaa acatttttgg attggtttag cacctccaga acgaaactat
     9961 ctctatcaac gtttgtctga tcagtatcgt gaagaggatt tactggattc aggcctgttt
    10021 tatctctcgg atgacaatca atttgtagac acctttcaca atcgcattat gtttcccctg
    10081 acaaatgacc aaggaaaggt cattgccttc tcaggtcgta tctggcaaaa aacggattca
    10141 caaacttcta agtataaaaa cagccgatcg actgcaattt ttaacaaaag ttacgaatta
    10201 tatcatatgg atagggcaaa aaaatcttct ggaaaagcta gtgagattta cttgatggaa
    10261 ggattcatgg atgttattgc agcctatcgg gctggaatcg aaagtgctgt ggcgtcgatg
    10321 ggaacggcct tgagtcgaga gcatgttgag catctgaaaa ggttaaccaa gaaattggtt
    10381 cttgtttacg atggagataa ggctgggcaa gccgcgacat tgaaagcatt ggatgaaatt
    10441 ggtgatatgc ctgtgcaaat cgtcagcatg cctgataact tggatcctga tgagtatcta
    10501 caaaaaaatg gtccagaaga cttggcctat ctattaacga aaactcgtat tagtccgatt
    10561 gagttctaca ttcatcagta caaacctgaa aacagtgaaa atctgcaggc tcagattgag
    10621 tttattgaaa aaatagctcc cttgattgtt caagaaaagt ccatcgctgc tcaaaacagc
    10681 tatattcata ttttagctga cagtctggcg tcctttgatt atacccagat tgagcagatt
    10741 gttaatgaga gtcgtcaggt gcaaaggcag aatcgcatgg aaggaatttc cagaccgacg
    10801 ccaatcacca tgccaatcac caagcagtta tcggctatta tgagggcaga agcccatcta
    10861 ctctatcgga tgatggaatc ccctcttgtt ttgaacgatt accgtttgcg agaagacttt
    10921 gcatttgcta cacctgaatt tcaggtctta tatgacttgc ttggccagta tggaaatctt
    10981 cctccagaag ttttagcaga gcagacagag gaagttgaaa gagcttggta ccaagtttta
    11041 gctcaggatt tgcctgctga gatatcgccg caggaactta gtgaagtaga gatgactcga
    11101 aacaaggctc tcttgaatca ggacaatatg agaatcaaaa agaaggtgca ggaagctagc
    11161 catgtaggag atacagatac agccctagaa gaattggaac gtttaatttc ccaaaagaga
    11221 agaatggagt aataatggca acaaaacaaa aagaagtaac aacatttgac gtacaggtag
    11281 cagaatttat ccgtaatcat aagcaaaaag ggacagcaac agatgatgaa atcaatgcaa
    11341 gtctggttat tccttttacc ttggacgctg atgggattga agatctcttg caacggattc
    11401 aggatgcagg aatttctatc acagataacg aaggaaatcc aagtgcgcgt gttcttagca
    11461 atgaagaaga accagaactc agcgatgagg acttgattgg gtcaacttct gctaaggtca
    11521 atgaccctgt ccgtatgtac ttgaaagaaa taggggtcgt tcctctcttg accaatgaag
    11581 aggagaaaga gttggcactg gctgttgaag ctggtgatat cgaagccaaa caacgtcttg
    11641 cggaagccaa tcttcgtttg gttgtttcca ttgccaaacg ctatgtcggt cgtggcatgc
    11701 agttccttga cttgattcaa gaaggaaata tgggcttgat gaaggcggtt gacaagtttg
    11761 actattctaa agggttcaag ttttcaactt atgcaacttg gtggattcgt caggctatca
    11821 ctcgtgctat tgcggaccaa gctcgtacca tccgtatccc agttcacatg gttgaaacta
    11881 tcaataaatt ggttcgtgaa cagcggaatc tccttcaaga attggggcaa gatccgacac
    11941 cagaacagat tgctgaacga atggatatga cacctgataa ggttcgtgaa atcttgaaga
    12001 ttgcccaaga accagtatct cttgaaactc ctatcggtga agaggacgat agccaccttg
    12061 gagactttat cgaagatgaa gtgattgaaa atccagtgga ttatacgact cgtatcgtct
    12121 tgcgtgagca attggatgaa atcttagata ctcttacaga ccgtgaagaa aatgttctgc
    12181 gtctacgttt tggactagat gatggaaaaa tgcgcacact tgaagatgtg gggaaagtct
    12241 ttaacgtaac tcgtgagcgt atccgtcaga ttgaagcaaa ggctttgaga aaactacgcc
    12301 aaccaagtcg tagcaaaccg cttcgtgatt ttattgaaga ctaagagtga ggaaaatatg
    12361 gcttatacag aagagcaaat tgaaaacatc aaaacacgga ttttaacagc cttggaagaa
    12421 gtcatcgacc ctgagttggg aatcgatatt gtcaatcttg gtttgatcta tgagattcgt
    12481 tttgacggtg acacagggca aacagagatt gacatgactt tgacaactat gggttgtccc
    12541 ttagcagacc ttttgacaga ccagatttat gatgctatga tagaggttcc agaagtaacg
    12601 gatactgagg ttaaattagt ctggtatcca gcctggacag ttgaaaaaat gagtcgctat
    12661 gcccgcattg ccctaggcat taagtaaaca tataaaagca tgtgagagac tgttggaaaa
    12721 gcgaggaaat ttcccctctt ttcctctagt ctctcctttc ttttgctgat tttattcaaa
    12781 gaaaatgata taatagtagt tatggagaaa aagaaattac gcatcaatat gttgagttca
    12841 agtgagaaag tagcaggaca gggagtttca ggtgcttacc gtgaattagt tcgtcttctt
    12901 caccgtgctg ccaaggacca attgattgtt acagaaaatc ttccaatcga ggcagatgtg
    12961 actcactttc atacgattga ttttccctat tatttatcaa ccttccaaaa gaaacgctca
    13021 gggagaaaga ttggctatgt gcatttcttg ccagctacac ttgagggaag tttgaaaatt
    13081 ccatttttct taaagggaat tgtgaaacgc tatgtatttt ctttttacaa ccggatggag
    13141 cacttggttg tggtcaatcc tatgtttatt gaggatttgg tagcagctgg tattccacgt
    13201 gaaaaagtga cctatattcc taactttgtc aacaaggaaa aatggcatcc tctaccacaa
    13261 gaagaggtag tcagactgcg cacagatctt ggtcttagtg acaatcagtt tatcgtagta
    13321 ggtgctgggc aagttcagaa acgtaaaggg attgatgact ttatccgtct ggctgaggaa
    13381 ttgcctcaga ttacctttat ctgggctggt ggcttctctt ttggtggtat gacagatggt
    13441 tatgaacact ataagaaaat tatggaaaat ccccctaaaa atttgatttt tccaggcatt
    13501 gtatcgccag agcggatgcg cgaattgtat gctctagcgg atcttttctt gttgcctagt
    13561 tacaatgagc tctttcctat gactatttta gaagctgcga gttgtgaggc tcctattatg
    13621 ttgcgtgatt tagatctcta taaggtgatt ttggagggaa attatcgggc gacagcgggt
    13681 agagaagaga tgaaagaggc tattttggaa tatcaagcaa atcctgctgc cttaaaagat
    13741 ctcaaagaaa aggctaagaa tatttccaga gagtattctg aagagcatct gttacaaatc
    13801 tggttggact tttatgagaa acaagccgct ttagggagaa agtaaaaagt gaggtaatct
    13861 atgcgaattg gtttatttac agatacctat tttcctcaag tttctggtgt tgcgaccagt
    13921 attcgaacct tgaaaacaga acttgaaaag cagggacatg ctgtttttat ctttacgacg
    13981 acagataagg atgtcaatcg ctacgaagat tggcaaatta tccgcattcc aagtgttcct
    14041 ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt
    14101 gctaaacagt atcagctaga tattatccat actcagacag aattttctct tggcctgttg
    14161 gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc atacctatca cacccagtat
    14221 gaagactatg tccattatat tgctaagggg atgttgatcc ggccgagtat ggtcaagtat
    14281 ctggttagag gtttcctgca tgatgtggat ggggttattt gtcctagtga gattgtccgt
    14341 gacttgctat ctgattataa ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa
    14401 ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa
    14461 ctagggattc aagatggtga aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa
    14521 aatattcaag cagttttagc agcctttgct gatgttctga aagaggaaga caaggttaaa
    14581 ctggtagtag ctggggatgg cccttatctg aatgacctca aagagcaagc ccagaaccta
    14641 gagattcaag actcagtcat ctttacaggg atgattgctc ctagtgagac ggctctttac
    14701 tataaagcgg cggatttctt catttcggca tcgacaagcg aaacgcaagg tttgacctac
    14761 ttggaaagct tagccagtgg aacacctgtc attgctcacg gaaatcctta tttgaacaac
    14821 ctcatcagtg ataaaatgtt tggaaccttg tactatggag aacatgattt ggctggtgct
    14881 attttggaag ccctgattgc aacaccagac atgaacgagc ataccttatc agagaaattg
    14941 tatgagattt cagctgagaa ctttgggaaa cgagtgcatg agttttatct ggatgccatt
    15001 atttcaaata acttccagaa agatttggct aaagatgata cggtcagtca gcgtatcttt
    15061 aagacagttt tgtatcttcc gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc
    15121 atgttgaagg cttcaaaaac acagttgatc agtatgagag actattggaa agaccatgaa
    15181 gaatagaaag aggaacagct atgaaaaaaa caattaatga gaagcggtcg tgataaaaag
    15241 attgcgggtg tttgtgctgg ggtggcccat tatctggata tggatccgac tatcgttcaa
    15301 gtcatttggg gtgttcttac ttgctgttac ggagctggaa ttgtagctta cattatttta
    15361 tggattatcg caccagtagc aactgactat tgaaaactag ctcaattcat gctaagataa
    15421 tagaaaatag aatgtaacga aggagagaaa aatggcattt ggagataatg gaaatcgtaa
    15481 aaaaactatg tttgagaaaa taaccttgtt tatcgtgatt atcatgctag tagcaagttt
    15541 attgggaatt tttgcaactg caattggtgc cctcagtaat ctataaaata gattcaagaa
    15601 aatttagtga ctgggatttc ccagcccttt tttaaagtga gaagaaataa tgagtatgtt
    15661 tttagataca gctaagatta aggtcaaggc tggtaatggt ggcgatggta tggttgcctt
    15721 tcgtcgtgaa aaatatgtcc ctaatggagg cccttggggt ggtgatggtg gtcgtggagg
    15781 caatgtggtc ttcgttgtag acgaaggact acgtaccttg atggatttcc gctacaatcg
    15841 tcatttcaag gctgattctg gtgaaaaagg gatgaccaaa gggatgcatg gtcgtggtgc
    15901 tgaggacctt agagttcgag taccacaagg tacgactgtt cgtgatgcgg agactggcaa
    15961 ggttttaaca gatttgattg aacatgggca agaatttatc gttgcccacg gtggtcgtgg
    16021 tggacgtgga aatattcgtt tcgcgacacc aaaaaatcct gcaccggaaa tctctgaaaa
    16081 tggagaacca ggtcaggaac gtgagttaca attggaacta aaaatcttgg cagatgtcgg
    16141 tttagtagga ttcccatctg tagggaagtc aacactttta agtgttatta cctcagctaa
    16201 gcctaaaatt ggtgcctacc actttaccac tattgtacca aatttaggta tggttcgcac
    16261 ccaatcaggt gaatcctttg cagtagccga cttgccaggt ttgattgaag gggctagtca
    16321 aggtgttggt ttgggaactc agtttctccg tcacatcgag cgtacacgtg ttatccttca
    16381 catcattgat atgtcagcta gcgaaggccg tgatccatat gaggattacc tagctatcaa
    16441 taaagagctg gagtcttaca atcttcgcct catggagcgt ccacagatta ttgtagctaa
    16501 taagatggac atgcctgaga gtcaggaaaa tcttgaagaa tttaagaaaa aattggctga
    16561 aaattatgat gaatttgaag agttaccagc tatcttccca atttctggat tgaccaagca
    16621 aggtctggca acacttttag atgctacagc tgaattgtta gacaagacac cagaattttt
    16681 gctctacgac gagtccgata tggaagaaga agcttactat ggatttgacg aagaagaaaa
    16741 agcctttgaa attagtcgtg atgacgatgc gacatgggta ctttctggtg aaaaactcat
    16801 gaaactcttt aatatgacca actttgatcg tgatgaatct gtcatgaaat ttgcccgtca
    16861 gcttcgtggt atgggggttg atgaagccct tcgtgcgcgt ggagctaaag atggggattt
    16921 ggtccgcatt ggtaaatttg agtttgaatt tgtagactag gagactggta tgggagataa
    16981 accgatatct ttccgagatg cggatggtaa ttttgtttcc gccgcagacg tttggaatga
    17041 aaagaaattg gaagaactat ttaatcgtct caatccaaat cgtgccttga gattggcacg
    17101 aactaaaaag gaaaatccat ctcagtaaag aagctaaaaa atcc
//
LOCUS       DAVMHG010000035        17092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMHG010000035 DAVMHG010000000
VERSION     DAVMHG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.035"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            79..530
                     /locus_tag="VYR08_000903"
                     /pseudo
     CDS             79..530
                     /locus_tag="VYR08_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            695..994
                     /locus_tag="VYR08_000904"
     CDS             695..994
                     /locus_tag="VYR08_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV3466456.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1255..1542
                     /gene="tnpB"
                     /locus_tag="VYR08_000905"
                     /pseudo
     CDS             1255..1542
                     /gene="tnpB"
                     /locus_tag="VYR08_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1628..2710)
                     /locus_tag="VYR08_000906"
                     /pseudo
     CDS             complement(1628..2710)
                     /locus_tag="VYR08_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2845..3126
                     /locus_tag="VYR08_000907"
     CDS             2845..3126
                     /locus_tag="VYR08_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV3466457.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            3258..4202
                     /gene="manA"
                     /locus_tag="VYR08_000908"
     CDS             3258..4202
                     /gene="manA"
                     /locus_tag="VYR08_000908"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV3466458.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(4223..5490)
                     /locus_tag="VYR08_000909"
                     /pseudo
     CDS             complement(4223..5490)
                     /locus_tag="VYR08_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            5791..6536
                     /locus_tag="VYR08_000910"
                     /pseudo
     CDS             5791..6536
                     /locus_tag="VYR08_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(6565..7020)
                     /locus_tag="VYR08_000911"
     CDS             complement(6565..7020)
                     /locus_tag="VYR08_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3466459.1"
                     /translation="MNRREAVEFVNMCMIKNGDKILVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCSIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(7040..8215)
                     /locus_tag="VYR08_000912"
     CDS             complement(7040..8215)
                     /locus_tag="VYR08_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV3466460.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSLVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(8274..9119)
                     /locus_tag="VYR08_000913"
     CDS             complement(8274..9119)
                     /locus_tag="VYR08_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3466461.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            9244..9801
                     /locus_tag="VYR08_000914"
     CDS             9244..9801
                     /locus_tag="VYR08_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3466462.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            9820..10287
                     /locus_tag="VYR08_000915"
     CDS             9820..10287
                     /locus_tag="VYR08_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV3466463.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            10376..11005
                     /gene="upp"
                     /locus_tag="VYR08_000916"
     CDS             10376..11005
                     /gene="upp"
                     /locus_tag="VYR08_000916"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV3466464.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            11179..11769
                     /gene="clpP"
                     /locus_tag="VYR08_000917"
     CDS             11179..11769
                     /gene="clpP"
                     /locus_tag="VYR08_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV3466465.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            11848..12096
                     /locus_tag="VYR08_000918"
     CDS             11848..12096
                     /locus_tag="VYR08_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV3466466.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            12198..13358
                     /locus_tag="VYR08_000919"
     CDS             12198..13358
                     /locus_tag="VYR08_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV3466467.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            13626..14495
                     /locus_tag="VYR08_000920"
     CDS             13626..14495
                     /locus_tag="VYR08_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3466468.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            14499..15455
                     /locus_tag="VYR08_000921"
     CDS             14499..15455
                     /locus_tag="VYR08_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV3466469.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            15455..16219
                     /locus_tag="VYR08_000922"
     CDS             15455..16219
                     /locus_tag="VYR08_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466470.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKVKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            16219..16929
                     /locus_tag="VYR08_000923"
     CDS             16219..16929
                     /locus_tag="VYR08_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466471.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQETTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
BASE COUNT         5247 a         3345 c         3572 g         4928 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttta ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagtg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaga accaaatggt gcttggttcc taccatgtgg aaaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttccctattg acgaggctat caaaaacctt aaacgtggta
      481 gccataaatg ggccattgaa tcttggaaaa aacagcataa gattggctaa tactcttcga
      541 aaataaaatt caaatcacgt aagcgtcgcc ttaccgtact caagtatagc ctgcggctag
      601 cttcctagtt tgctctttga ttttcattga gtataaatca agcgactcta ttcaaaaagt
      661 gttataatag aattagaaaa tcggaggaaa tattatgaag cttatcaata cgacaaactc
      721 acactcgcaa cttgtaaaaa gtcaactaga aagtaccgat gcaacccttg ttgaggttta
      781 ttctgcaggg aatacagatg ttatttttac ccaagctccg cttcactatg aaatcctcat
      841 ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa accattcaag aatttttctt
      901 gaaacgtaaa attgataaga cctctgttga tgaagccaat atcaagacac tctactcaga
      961 gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat tgagtaaaaa ctcgatttga
     1021 gagaaagtcc aataagtctt tccataacaa aacgcatagt aacaagagtt tctacaccca
     1081 ctattatgcg tttttatcat tttgaaatct actgctaatc aatgaagaag caaagatcaa
     1141 actaggcagc tagttacaag ttacgtaagc gcgtcataac aaggtatcta tcctaattcc
     1201 ccaacttcca gtatactaga taaaaaataa ttatcggagg aaaggtattg tactatgata
     1261 attcaactaa gtgatttagg tcaagttcac cttgtttgtg gtaagacaga tatgaggtag
     1321 ggaatagact cattagccta tgtagttaaa acccactttg aattggattc tttctccggt
     1381 caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagcccttta ctgggatggt
     1441 caaggatttt ggctactata taaacgcttt gagaacggaa aactgacttg gctaagtgca
     1501 gaaaaggatg tcaaagctct cgcacctgaa caagtagatt gactgatgaa gggcttttct
     1561 atcactccaa aaataaatct atcagaaagt cgtgatttct attgaaatga ggactttctt
     1621 tttatactca tctgctttca aaaagcattc tattccatct ccgattaccg atggacttta
     1681 tcacctcctt ctccagtcct tgtatgacat cttgaagttg attcatgaca tcttccaaag
     1741 ttcgaaaggc tttattctta aatccacatt tacgaatctc tttccacact tgttcaatgg
     1801 ggttcatctc tggtgtgtat ggaggaataa atgcaaagcc aatattagtc ggaatcttta
     1861 agatacttga tttttgccat atagcattgt ccataacgag taaaagataa tcatctggat
     1921 aagcttgtga aagctcttct aaaaaggcgt tcatccactc agtattacat ccaccagcta
     1981 ttaagaaaaa tgattcgcct gtatgggcat caacagctcc ataacaatag cgaaattctc
     2041 gtatatagtg actatggata tgtggaccta ctcctattgg agcccaacaa gatcccagtt
     2101 tactgattct accgaaacca gcctcatctt ggtacatcaa gcgaacctta cgaaaacgtc
     2161 tactatattt aaagcgcttt cttatcttct tgaattgaga ttttattttt agacgcaaca
     2221 atggtttgag cgtctgcttt cttaggatgt tctggacgtg gcataatatt tcgccaacca
     2281 tggtgcttca acagttgata gaaggcatca cgtgtgtagg aacgacctaa ctccttttta
     2341 taagcctgaa ataaggcatc aattgtaaca aattctcctg cctctgcagc cttcaaatgg
     2401 cgggcaagaa aggctttctc ttcctcaact gtcatatatg catggttacg accaccacgt
     2461 gtttcttgaa ggagagagtc gagtccgaac tcctcatatt tttttacatt tcgccaaatc
     2521 attgtttgat tacagtctaa aagttctata atctctttat aagatttgcc catcagacga
     2581 aatataataa attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg
     2641 atttgactgt cctgatcgat ttgccctgtt cttgtttcgt tttactatag aacgatttga
     2701 aggcgtttat gatatttagc tgtacgagag tctttaaaaa gtgttttgat ggtttggatt
     2761 tcttctttag ttgatttcat atcactatta tataatgctt tttgatttta gtatagtatt
     2821 atcgtagaaa atggactcgg tgctatggat acaccggatg agaatggtta tgtagcagat
     2881 gactatcgga ttacttactt agaggcccac atcaaggcca tgcgagatgc catttacaaa
     2941 gacggggttg acttgcttgg ttatacgaca tggggctgta tcgattcggt ttcagctggc
     3001 acaggggaaa tgaataagcg ctatggcttt atctatgtag atcgagataa tgtaggaaat
     3061 gggactctca aacgtagcaa gaagaagtcc ttctattggt acatgtcatt tatagcaatg
     3121 gtgtaagcat cgagtagagc acatttgttt actattttta taaaatcttc tccctgcttt
     3181 ataagatgta aaaaagagtt caattcgaac tctttttttg ctataatgag gggagaaaaa
     3241 tcagacagga gatcgacatg tcagaaccat tatttttaca atcagttatg caagaaaaaa
     3301 tctggggtgg agccaagcta cgtgatgagt ttggctacga catcccaagt gaaaaaatcg
     3361 gagaatattg ggccatctca gcccatccaa atggagtctc taaagttgcc aatggtcgtt
     3421 acgagggaac agatcttgct actttgtatg cggaacaccg tgaattattt ggcaatcgtc
     3481 cagaacctgt atttccactt ttgaccaaga tcctcgatgc caacgactgg ctcagtgtcc
     3541 aagttcaccc agacgatgct tatggactcg agcatgaagg cgaactcgga aaaacagaat
     3601 gctggtacat tatcgcagcg gatgaaggtt cagagattat ctacggtcac aatgccaagt
     3661 caaaagaaga actccgccag caaatcgagg acaagaactg ggatgacttg ttgaccaaag
     3721 tccctgttaa ggctggagat ttcttctatg taccaagcgg gactatgcac gctatcgggg
     3781 cgggtatctt aatccttgaa acccagcagt ctagcgatac cacctatcgc gtctatgact
     3841 ttgaccgcaa ggatgacaaa ggcaacttgc gtgaacttca tcttgaaaaa tccatcgatg
     3901 tcttgaacat tggtgagcca gcaaatagcc gtcctgtaac tgttaaagca gatgatttgc
     3961 gttccactct ccttgtatct aatgatttct tcgcagttta caagtgggaa attactggaa
     4021 aagttgactt tgaaaagaca gctgactaca gcttattgag tgtcttagct ggtcaaggtc
     4081 agctaactgt tgacgggaaa aattatccaa ttcaaaaagg gagacacttt atcctaccaa
     4141 gtgatgttga agcttggact ctggaagggc aaggtttgga attgattgtt agccatccat
     4201 aaaaagaaag ggcctgagtt cactactcaa gtcctttttg attacataaa ttgggcaatg
     4261 aagaccacaa tgatgatgat tggaatgacg aaacgaagaa acgaagaaac gaagaaggaa
     4321 gagccagact tggaacagtc cttgtttcca tgctctttca tcgagatgga gttcctccat
     4381 tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa aggtagagag ctccaaatgg
     4441 catgaggaga ttggaagcca agaagtccat agcgtcaaag aagatcttac caaaaatgtg
     4501 aacatccgcc atgacaccgt aagatagggc tgaaggaatg ccaaagacaa aggtcaaaat
     4561 tcctaaaata acactccatt tggcacgttt gctgttatcc tggttggtga tattgcccac
     4621 attgatctcc agcatcacga cagaagaagt gacagtcgca aagaggaaaa ggacgtagaa
     4681 aatggttcca aaaggcatct tgtcaaagag ttgaggcaag acgataaaga gcaagctcgg
     4741 tcccccttca atatccagtc tgaaggggga ccgagcttgg aaaatggcta gacctgccat
     4801 gatggatatc gagatattca tggctacgat ggagattcct gactggacta gattggtttt
     4861 cttgtccaag taagaagcat aggtcagcat gactgtaacc cctagtgaga gggcaaagaa
     4921 agattgtccc agagcataga ggggaccagc gctagtcagt tttgaaaagt ctggtttgag
     4981 gaagtaaaga accccttcca tggcatttgg caaactggga gagcgttcga tgatgacgac
     5041 aaagatgata aagagcaggg gcatcatgac tttcgaagct ctttcaatcc ctttttgaac
     5101 cccacgtgat acaataaaga tatttaataa gataaaggct gcttgagctc ctagggcaat
     5161 ggctggattt gaaatgattg aagtaaataa ctgagcataa tcacccgttc caccaagttg
     5221 gaacaatttc ccaaactcaa tacctagata gactagaatc catcctccga taacactgta
     5281 aaaagataag aggataaaga gggcaaaggc gccaatccaa ccgataaagt tgtacttgtt
     5341 attcttgccc agttttccaa aggttttgat agcggaaacg ccagcactac ggccaagggc
     5401 aaactcagcc agcaggagag ggaaatcgat taaaatagtg gaaatgagaa agattagtaa
     5461 aaagcctcca ccgccattag cagcagtcat gtagggaaac ttccaaacgg caccaagccc
     5521 gatggccagc cagcagatgc tagaataaaa ccaagtttcg acccccattg tgatttttct
     5581 gacatagtta aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa
     5641 tagccttctg tttgattgat agacttgact atcctattat gttttctaag gtctgtcaag
     5701 aaaaccattt tcaagttttc acacctttct caaaaacgct tgactctgac ctaaggggag
     5761 gggttatact atcattgtaa ggaggaaatc atgtaccata taaaagaagc tgcgcagctt
     5821 tcaggtgtct ctgtcaagac cctgccctgc atcactatga caagatagga ctcttggtcc
     5881 ccttaaagtc ggaaaacggc tatcgaacct atagtcaaga ggatttggaa cgccttcagg
     5941 tcattcttta ctacaaatat ctaggctttt ctttagagaa aatagcagag ctgttaaagg
     6001 aagaaaggac agatttattg ccccatttaa ctaggcagtt ggactatcta actcgcgaaa
     6061 ggcaacatct ggataccttg atttccacct tgcaaaaaac tattcaagaa caaaaaggag
     6121 aaagaaaaat gaccattgag gaaaaattca cgggatttag ctatcaagac aatcaaaaat
     6181 accaccaaga agcggtagag aaatatggtc aagaagtcat gggacaagcg ctcgaacgcc
     6241 aaaaaggtca cgaagacgag gctacggccg cctttaacca agtctttcaa actttggcac
     6301 aaaatcttca agttggttta cctgcaacag caaccgaaaa ccaagagcaa gcagccaagc
     6361 tcttgcaagc tattcgcact tatggatttg actgctctat tgagatattc ggtcatatcg
     6421 gtaaaggtta cgtctacaac ccagagttta aggaaaacat tgacaagttt ggttctggaa
     6481 cagcccagta cacgtcagat gccattgcgg cttacgttca gacaaatgca gaataaatag
     6541 gctaggaatt tcctagccta ttttttactt caaatcataa agccagtcgt caccgttttt
     6601 gtagtaaaag aattcactga gatcttcttc tagaaacaca cgaagcatat cagacatatc
     6661 atcggttgca agttttagat gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc
     6721 gtctgaagac tggagttcac cagtaaagtg ttctgtcttg taaaaaagga cgacataacg
     6781 ataatccttg tcgtcatacc agtttttaat actacagagt tggggtttgg aaatgatcag
     6841 accagtttct tctttcactt cacgaatgac agcatcgaca aaggattcgc cacgttcaac
     6901 atgaccacca ggaaaagtaa tgccaggcca gtcgggacta actcggtctt ggaccaggat
     6961 cttatctccg tttttaatca tacacatgtt aacaaattcg actgcctctc ttctgttcat
     7021 tcttcacaac ctttaatctt taatcataat gcagacttcc cgccacccag ccggtacaga
     7081 gagcagaagt gatgttaaag ccacccgtgt gggcattgat atccataact tcgcctgcaa
     7141 agtggaggcc aggtaccagc ttactttcaa gggttttagg attgatttcc ttgagactga
     7201 ctccaccctt ggtaacaaag gactttgcaa gggacatttt tccagttaca ggaattttaa
     7261 gttctttaat ggactggaca agttgttctc gttccttttc agtcagctgt ttgacttttt
     7321 caggatatcc ttgtacaaaa aattcggcca agcgttctgg taacaaggtt tttaaagcgt
     7381 ttttcaagga tttttcccga ttttcttcta gaaatgtaac caagtccttc tcagaaagtt
     7441 gaggcaaaac atcgagtgag agaacctccc cacctttgac caagctagac atgcgtaggg
     7501 cagcaggacc tgacaaacca aagtgggtaa agagtaaatc atgagtgatg acatgcttac
     7561 cataacttag ggtcacatcg tccagagaaa tcccttgtaa ggctttatgt ggaaaatctg
     7621 ttaataaagg actttcagca gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa
     7681 tctcgtgacc aaaaccagtc gaaccagtcg aaggataaga cttaccacct gttgtgacaa
     7741 tgagtttctc acaagtgaag gtttgatccg ctgacttaag gacaaactgg tcatctactt
     7801 ttttaacaga aacgatttct atttgagtag caacttgacc acctagttca gtgattttct
     7861 tttccaaagc ttcgataata gtccgagact tgtcactggc tggaaagacg cgtccgtggt
     7921 cttcgacctt aagtttaaca ccattttctg taaaaaagtt gatgatgtca tgattatcga
     7981 actgggagaa aacactgtaa agaaagcgtc cgtttccagg aattccagct agcaggttgt
     8041 ctaagctacc attgttggtc acattgcaac gtcccccacc agtcccagct aatttttttc
     8101 caagtttccg atttttttcg atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg
     8161 tagccatcat accagcaggt cccccaccga tgacaatagt atcaaaatgt ttcatagctc
     8221 tattgtacca caaaaaaaca agagatgatg gtcacctctt gtcaagaatg caattaatca
     8281 atttcatagc ccatcagcaa accgccctct tctgcataga aactgcagag accagaggtt
     8341 ggtagaattt taatatccgc ttgtgggaag gtttcacgga ttcgctctga gagctgttga
     8401 caacattttt cgttattgcg ttgggccatg acaatacggc caccagcata tccagctttt
     8461 actaactcat cataggcagc ttgaactgat ttctttgatc cccttgcttt ttgtagcaat
     8521 tcgagagtcc cagtttcact agcttttccg accatacgaa tgttgagaag gccaacgacc
     8581 gtaccgataa gcttgctcaa acggccgttc ttcaccaagt tatcgacttt ggctaggaca
     8641 aagagcaact tagttttttc ttgataggcg gtgatagctt caaccacttc ttcaaaagac
     8701 aagccctggt caatcaagtc attcaatttt tctacgagta ggtcaacttc accaccagca
     8761 gataaactat caatcacatg aatcttagtg tcaggatggt cttccagata aatattcttt
     8821 gctagttgag cactattgtg actgccagaa agggtacctg tgatggttac taggaaaatg
     8881 tttttggcac cttcaaatgc tcgcaaatag tcatctgggc ttggacaagc cgattttgaa
     8941 gcttctgcag ttgcatacat ggtttccatc atttggtcaa tatcgagact ggcgtcatca
     9001 acaaagacct gatcagctac ttgaatggtt aaggggacgc ttacaaaggt tgtgttaata
     9061 gctggtgttg gcagttgacg ataatcacaa ccagagtcag caataatttt ccaagtcata
     9121 gaaattctcc atctttgtca gttatacatt gacaaaggtt ctgtcttttt ttacaattat
     9181 atcatgaaaa cccttgaaac aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat
     9241 gttatgtctg aacgtagaat ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa
     9301 gaatccaatt tattaaccag agaagccatt gaaacagccc tcttgcaact cttggaaaaa
     9361 aaggagctga ccaagattag tatttctgaa ttggtcaaac gtgcgggtgt ttcgcgtgcg
     9421 gccttttatc gcaattatga ctccaaagag gaaattttag agagcgtctt taaacgaact
     9481 gtccacaata ttatggaaca gctgcatcat tacgatttaa agacagacct ttatttggtt
     9541 tgggttcacc ttttccggga ggccagaaag gaagctagag tgattcaatt ggccttggat
     9601 taccatctgg aaaaaatctt tgtccaagcc atgcaggaat ttctagaaaa ataccatggg
     9661 aaatcaaaag gtgtcagctc ttatcttcat tccttctgga gctcagccat cgtctctgtc
     9721 cttctaaaat ggatcaagga tggcatgaag gtaccagctg aaaagattgc agatttaggt
     9781 ttaccatttt ttaaaaaata gagaaaaagg agaaaagaga tgactgaaaa aagactggcc
     9841 tgggatgagt attttgcagc ccaagcccta ctaattgcga atcgttccac ttgtaaacgt
     9901 gctaaagtgg gcgcgattct ggtaaaagat aataaggtta tttccactgg ttacaatggt
     9961 tcggtgtcag ggactgagca ttgtattgat cacgaatgtt tggtcattga aggccactgt
    10021 gttcgcaccc ttcacgctga ggtcaatgct atccttcaag gtgctgagcg tggtgttccc
    10081 aaaggcttta cagcctatgt aacccatttt ccttgtctca actgtacaaa acaattgctt
    10141 caggtcggct gcaagcgtgt ggtttatatc aaccagtacc gaatggacga ctacgcccaa
    10201 tacctttatc aagaaaaagg gacagaattg acccatttac cacttgagac agtacagaca
    10261 gctcttaaag aggctgatct aatgtaaaaa ttatcaaaaa taaatggttt agaaagattt
    10321 ttaaaccgtt ttttggtata ataagaagaa taaattgaaa gaaggaattc caaaaatggg
    10381 aaaaattgaa gttattaatc acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac
    10441 agatacttct acaaaagctt ttcgtgagct agtagatgag attgcaatgt tgatggggta
    10501 tgaagtactt cgtgatcttc cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac
    10561 agttcaaaaa caattggcag gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat
    10621 cgggatggtt gatggtctct tgagcttggt tccagctgct aaagttggcc acatcggtat
    10681 gtaccgtgat gaagaaacac ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat
    10741 tgaccaacgt caaatttttg tagtagaccc aatgttggca acaggtggct cagcaatctt
    10801 ggctgttgat tctcttaaaa aacgtggcgc atcaaatatc aaatttgtct gccttgtatc
    10861 tgctccagag ggtgtaaaag cccttcaaga agctcatcca gatgtagaaa tctttacagc
    10921 agccttggat gaacgtttga acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg
    10981 agaccgcttg ttcggtacaa aataagatcg aaaagaagaa tgacttggaa aagtatttcc
    11041 agtcacgaaa ggaggttggg tttttgtttc tgtctaatga aagcagagca aaaatttgac
    11101 cttttttgac caagatatta taatagtctt atcttcagtc atttgaccaa caaaattaaa
    11161 actcaaaagg agaaatgaat gattcctgta gttattgaac aaacaagccg tggagaacgt
    11221 tcttacgata tttactcacg tcttctcaaa gaccgcatca ttatgctgac aggtccggtt
    11281 gaagacaata tggctaactc tgttattgcc caactgcttt tcttggatgc ccaagatagt
    11341 acaaaagata tttaccttta tgtcaataca ccaggtggtt ctgtttcagc tggtttggca
    11401 atcgtagata ccatgaactt tatcaaggca gatgtccaaa ccattgttat gggaatggct
    11461 gcatctatgg gaacagtcat cgcatcaagt ggagcaaaag gcaaacgttt catgcttcca
    11521 aatgctgaat acatgattca ccaaccaatg ggcggtacag gtggtggtac ccaacaaact
    11581 gatatggcta tcgctgcaga acacttgctc aaaactcgta ataccttgga aaaaatcttg
    11641 gctgaaaatt caggtcagtc aatggaaaaa gtccatgcag atgcagaacg tgataactgg
    11701 atgagcgccc aggaaacact tgaatatggc tttattgatg aaattatggc caacaattca
    11761 ttgaactaat gatgatagaa ggcaaactcg actgggtttg ctttttttgg tataataggg
    11821 agagatttct tagaaagagg atttatcatg tttgaaaaag tcaatcgctc tggcttgatt
    11881 atctatcttt actataatcg tgatgccaaa aaactgcagg attatggaga tattacctat
    11941 cattccaaga aacatcgtta cttacaactc tatgttccaa ctcaagaagt ggagcaattg
    12001 gtcggacgct tgagcaagga aaaatttatt aaaaaagtta gggtttgtca tatccaagag
    12061 ttggaaacac cctttgtggg caatctttat cgataggaaa acgctatcat cgaaaaagtt
    12121 caaggaaaac attgacaatt ttctgataat tcggtatatt cttaacagac tatttaagaa
    12181 ataaggagac aaaaaagatg aagaaaaaat ttgccctatc gtttgtggcg cttgcaagtg
    12241 tagcacttct tgcagcctgt ggagaagtga agtctggagc agtcaacact gctggtaact
    12301 cagtagagga aaagacaatt aaaatcgggt ttaactttga agaatcaggt tctttagctg
    12361 catacggaac agctgaacaa aaaggtgccc aattggctgt tgatgaaatc aatgccgcag
    12421 gtggtatcga tggaaaacaa atcgaagtag tcgataaaga taataagtct gaaacagctg
    12481 aggctgcttc agttacaact aaccttgtaa cccaatctaa agtatcagca gtcgtaggac
    12541 ctgcgacatc tggtgcgact gcagctgcgg tagcgaacgc tacaaaagca ggtgttccat
    12601 tgatctcacc aagtgcgact caagatggat tgactaaagg tcaagattac ctctttattg
    12661 gaactttcca agatagcttc caaggaaaaa ttatctcaaa ctatgtttct gaaaaattaa
    12721 atgctaagaa agttgttctt tacactgaca atgccagtga ctatgctaaa gggattgcca
    12781 aatctttccg cgagtcatac aagggtgaaa tcgttgcaga tgaaactttc gtagcaggtg
    12841 acacagactt ccaagcagcc cttacaaaaa tgaaagggaa agactttgat gctatcgttg
    12901 ttcctggtta ctataatgag gctggtaaga ttgtaaacca agcgcgtggt atgggaattg
    12961 acaaaccaat cgttggtggt gatggattca acggtgagga gtttgtacaa caagcaactg
    13021 ctgaaaaagc atcaaacatc tactttatct caggcttctc aactactgta gaagtttcag
    13081 ctaaagctaa agccttcctt gacgcttacc gtgctaagta caatgaagag ccttcaacat
    13141 ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa cgcagcaaaa ggtgctaaaa
    13201 attcaggtga aatcaagaat aaccttgcta aaacaaaaga ttttgaaggt gtaactggtc
    13261 aaacaagctt cgatgcagac cacaacacag tcaaaactgc ttacatgatg accatgaaca
    13321 atggtaaagt tgaagcagca gaagttgtaa aaccataata gaaaaatgtt gaaataggga
    13381 atgagccttt gactcactcc ctgtttcgat atttaatact cttcgaaaat ctcttcaaac
    13441 tgcgtcaacg tcgccttgga ttatatatgt gactgacttc gtcagtctta tctacaacct
    13501 caaagcagtg ctttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg
    13561 agtataagaa cctatcaaaa agtgagggaa acccctcgga attataaata gaaagagtga
    13621 atcttatgct ccaacaactc gtaaatggtt tgattctagg tagtgtttac gcgctgttag
    13681 ccctaggata taccatggtt tacggaatta tcaagctcat caacttcgcc catggtgata
    13741 tttatatgat gggagccttt atcggttatt tcttgatcaa ttctttccaa atgaatttct
    13801 ttgtagcgct tattgtagct atgctagcga cagctattct tggtgtcgtg attgagtttc
    13861 ttgcttaccg acctttgcgc cactctactc gtattgctgt tttgattacg gctattgggg
    13921 tttctttcct attggagtat ggaatggtct atctggttgg tgccaatacc cgtgccttcc
    13981 ctcaagcgat tcaaacagtt cgatatgatt tgggaccaat tagcttaaca aatgtgcagt
    14041 taatgatttt ggctatttcc ttgattttga tgattttgtt acaagtcatt gtccaaaaga
    14101 ctaagatggg gaaagccatg cgtgcagtat cagtagatag cgacgcggcg caattgatgg
    14161 ggatcaatgt aaaccgtacg attagcttta ccttcgcttt gggttctgct cttgcgggtg
    14221 cggctggtgt tctgattgct ctttattata actctcttga gcctttgatg ggggttactc
    14281 caggtcttaa atctttcgtt gccgcagtac ttggtggtat cggaattatt cctggtgcgg
    14341 ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc gactgccttt gggatgtcag
    14401 atttccgtga tgccattgtt tatggaatct tgttgttgat cttgattgtc cgcccagctg
    14461 gtatccttgg taagaatgtg aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta
    14521 atattctatg gttactcctt ttgttagctg gctatagctt gattagtgta ctggtttcag
    14581 tcggagtact taatctattc tatgtacaga ttttacagca aattggaatt aatattattt
    14641 tggctgttgg tctcaactta atcgttggtt tttcaggaca attttcactt ggtcatgctg
    14701 gtttcatggc gattggtgcc tatgcagcag ctattattgg ttctaaatta ccaacctacg
    14761 gtgccttctt tggagctatg cttgtagggg ctttgctttc aggagcagtt gccttacttg
    14821 tcggcattcc aaccttgcgc ttgaaggggg actatcttgc ggtagcaact ctgggtgttt
    14881 ctgaaattat ccgtatcttt atcatcaatg gtggaagcct tacaaatggt gcggcaggta
    14941 tcttagggat tcctaacttt acaacttggc aaatggttta cttctttgtc gtgattacaa
    15001 ccattgcaac cttgaacttc ttgcgtagcc caattggtcg ttcaaccctc tctgttcgtg
    15061 aagatgaaat cgctgctgag tcagttgggg ttaatacgac taaaattaaa atcatcgctt
    15121 ttgtctttgg tgccattact gcaagtattg ctgggtcact tcaggcagga tttatcgggt
    15181 ctgttgtacc gaaagattac accttcatca actcaatcaa cgttttgatt attgttgtat
    15241 ttggtggact cggttccatt acaggtgcga ttgtttcggc tattgttctg ggaattttga
    15301 atatgcttct ccaagatgtt gctagtgtgc gtatgattat ttacgctttg gccttggtat
    15361 tggtaatgat tttcagacca ggtggactcc ttggaacatg ggaattgagc ctatcacgtt
    15421 tctttaaaaa atctaagaag gaggaacaaa actaatggca ttacttgaag taaaacagtt
    15481 aaccaaacat tttggtggtc taacagctgt tggagatgtg actcttgaat tgaacgaagg
    15541 ggaactggtt ggattaatcg gtccaaacgg agctgggaaa accacccttt tcaacctttt
    15601 gaccggtgtt tatgaaccaa gtgagggaac agtaacccta gatggtcacc ttttgaatgg
    15661 gaaatcacct tataagattg cctctttggg acttggacgt actttccaaa atatccgtct
    15721 ttttaaagat ttaacagttt tagacaatgt tttgattgct tttggaaacc atcacaaaca
    15781 gcatgttttt actagtttct tacgcttacc agctttttac aagagtgaaa aagaattaaa
    15841 ggttaaagct ttggaattgt tgaaaatctt tgatttagat ggtgatgcag agactcttgc
    15901 taaaaatctt tcctacggac aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga
    15961 acctaaaatt ctcttcttag atgaaccagc agcaggtatg aacccacagg aaacagccga
    16021 attgactgag ttaattcgtc gtatcaaaga tgagtttaag attacaatca tgttgattga
    16081 acacgatatg aatctggtca tggaagtaac agaacgtatc tacgtacttg aatatggccg
    16141 tttaatcgct caaggaactc cagacgaaat taagaccaat aaacgcgtta tcgaagctta
    16201 tctaggaggt gaagcctaat gtctatgtta aaagttgaaa atctttctgt gcattacggt
    16261 atgatccaag cagttcgtga tgtaagcttt gaagttaatg aaggagaagt tgtttccctt
    16321 atcggtgcca acggtgcagg taagacaact attcttcgca ccttgtcagg tttggttcga
    16381 ccaagttcag gaaagattga atttttaggt caagaaatcc aaaaaatgcc agctcagaaa
    16441 atcgtggcaa gtggtctttc acaagttcca gaaggacgcc acgtctttcc tggcttgact
    16501 gttatggaaa atcttgaaat gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct
    16561 aacttgaaga aggttttctc acgctttcct cgtcttgaag aacggaagaa ccaagatgca
    16621 gccactcttt caggggggga acaacaaatg cttgccatgg gacgcgccct catgtcaaca
    16681 ccaaaacttc ttcttttaga tgaaccatca atgggacttg ccccaatctt tatccaagaa
    16741 atttttgata tcattcaaga tattcagaag caagaaacaa cggtcctctt gattgaacaa
    16801 aatgccaata aagcacttgc aatctctgac cgaggatatg tactggaaac agggaaaatc
    16861 gtcctatcag gaacaggaaa agaactcgct tcatcagaag aagtcagaaa agcatatcta
    16921 ggtggctaaa acaatccagt ggattgtttt agtcggcaga tggagattac gaagtaatca
    16981 tcaatatagt ccgggggacc tttttagtcg gtagattgag attgcaaaca aatctgcatc
    17041 tacattgaaa gcttaatttc taataattga aaaaatcgaa tgaaaaattt ct
//
LOCUS       DAVMHG010000036        17028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMHG010000036 DAVMHG010000000
VERSION     DAVMHG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.036"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1137..1376
                     /locus_tag="VYR08_000924"
     CDS             1137..1376
                     /locus_tag="VYR08_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466472.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1361..1639
                     /locus_tag="VYR08_000925"
     CDS             1361..1639
                     /locus_tag="VYR08_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466473.1"
                     /translation="MYNDVIERISLCEFIGDIFYSKITSCCIVAKDVSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            1925..4249
                     /gene="pspA"
                     /locus_tag="VYR08_000926"
     CDS             1925..4249
                     /gene="pspA"
                     /locus_tag="VYR08_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV3466474.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEAKADKEAKASAEIDKATFA
                     VQSAYVKFLNVQSNRQISENERKKQLAEIDKEIENAKQNLQNKQEEFNKVRAEVIPEA
                     KGLAVTKQKAEEAKKEAEVAKKKFEEAAKEVEVAKKEVEAKELEIEKLQDEISTLEQE
                     VATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKLLDSL
                     DPEGKTQDELDKEAAEAELNKKVESLQNKVADLEKEISNLEILLGGADSEDDTAALQN
                     KLAAKQAELAKKQTELEKLLDNLDPEGKTQDELDKEAAEAELDKKADELQNKVADLEK
                     EISNLEILLGGADPEDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAP
                     APQPEKPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPTPAPKPEQPTPAPKP
                     EQPTPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQYNGSW
                     YYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNDSWYYLNASGAMATGWAKVNG
                     SWYYLNANGSMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQY
                     NGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQW
                     FKVSDKWYYVNGLGALAVNTTVDGYTVNENGEWV"
     gene            4650..5771
                     /gene="mnmA"
                     /locus_tag="VYR08_000927"
     CDS             4650..5771
                     /gene="mnmA"
                     /locus_tag="VYR08_000927"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV3466475.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGIMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5912..6367
                     /locus_tag="VYR08_000928"
     CDS             5912..6367
                     /locus_tag="VYR08_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3466476.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6377..8290
                     /gene="mnmG"
                     /locus_tag="VYR08_000929"
     CDS             6377..8290
                     /gene="mnmG"
                     /locus_tag="VYR08_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV3466477.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYVAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(8624..10303)
                     /locus_tag="VYR08_000930"
     CDS             complement(8624..10303)
                     /locus_tag="VYR08_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV3466478.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10305..10538)
                     /locus_tag="VYR08_000931"
     CDS             complement(10305..10538)
                     /locus_tag="VYR08_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV3466479.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11307..11459)
                     /gene="cibB"
                     /locus_tag="VYR08_000932"
     CDS             complement(11307..11459)
                     /gene="cibB"
                     /locus_tag="VYR08_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV3466480.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11461..11646)
                     /gene="cibA"
                     /locus_tag="VYR08_000933"
     CDS             complement(11461..11646)
                     /gene="cibA"
                     /locus_tag="VYR08_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV3466481.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11824..12507
                     /gene="tsaB"
                     /locus_tag="VYR08_000934"
     CDS             11824..12507
                     /gene="tsaB"
                     /locus_tag="VYR08_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV3466482.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12504..12941
                     /gene="rimI"
                     /locus_tag="VYR08_000935"
     CDS             12504..12941
                     /gene="rimI"
                     /locus_tag="VYR08_000935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV3466483.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSSWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12931..13941
                     /gene="tsaD"
                     /locus_tag="VYR08_000936"
     CDS             12931..13941
                     /gene="tsaD"
                     /locus_tag="VYR08_000936"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV3466484.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVKPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(13987..15287)
                     /locus_tag="VYR08_000937"
                     /pseudo
     CDS             complement(13987..15287)
                     /locus_tag="VYR08_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15626..15914)
                     /locus_tag="VYR08_000938"
                     /pseudo
     CDS             complement(15626..15914)
                     /locus_tag="VYR08_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16045..16314)
                     /locus_tag="VYR08_000939"
                     /pseudo
     CDS             complement(16045..16314)
                     /locus_tag="VYR08_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16557..>17028
                     /locus_tag="VYR08_000940"
     CDS             16557..>17028
                     /locus_tag="VYR08_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV3466485.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFD"
BASE COUNT         5218 a         3170 c         3834 g         4806 t
ORIGIN      
        1 taatagaaac ataggttaaa tcttagaacg gcttaaaatc agatatgata aagggaaata
       61 gtcttatcta ggtaactaag tgctacaaac acagtcgagg atcacttgtt aggattacat
      121 tttaatcatg cttaataaga atggagaatt cctatgggga ttgtattact ctgaagtttc
      181 gaatacctta gttctcgtga ttttgagcta ttctagcttt agaatccttc agaatgtaaa
      241 attttaagca gtcaaagact tagaatagtc cgaaaacatt tattttatag caattctaag
      301 attagaattg ctcatatata tgtatttgag ctgctcgata gttaggacag ctctattctg
      361 agaaatctga gctatcaatg cttcagaata gtatttcgcc aatggttttt attgaagagg
      421 tgattgtgtt atgatagtct aaaataaaac tgatttaatg gagtaaatgg agaatcgtta
      481 catgaaacat ttttttgcag gaattggtga gataaaactt gttggctact tattgtatat
      541 atgtgtagga ctagcccttc tttttcatat atatgtcata ggttcggaac tgagttttgt
      601 gaaaatagtg ttatctattt tgggagttat atttgtaagt atgttaacta ttgctcgaat
      661 ttatcggact tttctataaa gaatagtttg gacttgaact gtatctaaaa acagaggttg
      721 gtcgattatc agcctctttc ttgtcgttga aaagagtgag atatattgac ttttgaaaaa
      781 aagatgtcat aattgagttg ggataaacct aaaagtaata tagtagattg aatctggaat
      841 agtccgttat gactgttaaa atatttctag aaattaattt gactttccta atcgatttgt
      901 tcacatcttg tttcaatcta ctatactttc tcgttaaacc gttcggcaat tcatggtgac
      961 atggacacat agtagtaagc acactatgtg gcttggcaga gctaaaaact gttcccttgc
     1021 gataagccta ataagcacaa catagggagt tagagaagcc gactctaatc aaccactttg
     1081 ggcagtagtg agaactgccc cgtgcttttt attttgagaa aatatggagt ttgtcgttga
     1141 aattacttga ttgtattttg gactatcaag aaagattcaa tggaaaaaca tgtcaagtat
     1201 caacgaatta taagtattta gagattttca aagtgaattt ttgcttgact gatttacatc
     1261 atttatttgg cttacacaaa atcacacgag attatgctag tcaaacaaaa cctgctattc
     1321 aagctggtgt ttttatttta gaagatttta gaaatatcct atgtacaatg atgtaatcga
     1381 aaggatatct ttatgtgagt ttatcggcga tattttctat tctaaaataa caagttgttg
     1441 catcgtagct aaggatgtat ctaaaaatac tatgaaattg gacgtcatat tttttgagga
     1501 taaaaataaa agatccgcag ttttaggttt acgaagagac aaaagcggag tatttaaacc
     1561 agttactcta cattttacaa gcgctaagaa atatgctaaa gttcgtaaaa cagatgtgaa
     1621 agaaatgaaa tggctataaa gattgtccgc aggcttaagc ttgcgttttt tgttgtttaa
     1681 cgaaattaaa aaacaagctc tctcatcgga aatgttttta taggaatgaa ggaagatgat
     1741 gctatgttta aaaagagaat cgaaaagggc aaaagtagtg tctttatctt tttggagtag
     1801 aagcttatga tatagaaatt tgtaataaaa atgtaatata aaacatttga caaatattta
     1861 cggggggagt atacttaata taagtatagt ctaaaaatga ctatcagaaa agaggtaaat
     1921 ttagatgaat aagaaaaaaa tgattttaac aagtctagcc agcgtcgcta tcttaggggc
     1981 tggttttgtt gcgtctcagc ctacttttgt aagagcagaa gaagctccac aagttgtcga
     2041 aaaatcttca ttagagaaga aatatgagga agcaaaagca aaagctgata ctgccaagaa
     2101 agattacgaa acggctaaaa agaaagcaga agacgctcag aagaaatatg atgaggatca
     2161 gaagaaaact gaggcaaaag cggataagga agcaaaagca tctgcggaaa tagataaagc
     2221 tacgtttgct gtacaaagtg cgtatgtaaa atttttaaat gtccaatcta atcgtcaaat
     2281 ttcggagaat gaacgaaaaa aacaattagc agaaatagat aaagagatag agaatgctaa
     2341 acaaaattta cagaataaac aggaagaatt taataaggtt agagcagaag taattcctga
     2401 agcaaagggg ttagctgtta ctaaacaaaa agcggaagaa gctaaaaaag aagcagaagt
     2461 agctaagaaa aaatttgaag aggcagctaa agaggtagaa gtagcgaaga aagaagtaga
     2521 ggctaaggaa cttgaaattg aaaaacttca agatgaaatt tctactttgg aacaagaagt
     2581 tgctactgct caacatcaag tagacaattt gaaaaaactt cttgctggtg tggatcctga
     2641 tgatacagaa gctatagaag ctaaattaaa aaaaggagaa gctgagctaa acgctaaaca
     2701 agctgagtta gcaaaaaaac aaacagaact tgaaaaactt cttgacagcc ttgatcctga
     2761 aggtaagact caggatgaat tagataaaga agctgctgaa gctgagttga ataaaaaagt
     2821 tgaatctctt caaaataaag ttgctgattt agaaaaagaa attagtaacc ttgaaatatt
     2881 acttggaggg gctgattctg aagatgatac tgctgctctt caaaataaat tagctgctaa
     2941 acaagctgag ttagcaaaaa aacaaacaga acttgaaaaa cttcttgaca accttgatcc
     3001 tgaaggtaag actcaggatg aattagataa agaagctgct gaagctgagt tggataaaaa
     3061 agctgatgaa cttcaaaata aagttgctga tttagaaaaa gaaattagta accttgaaat
     3121 attacttgga ggggctgatc ctgaagatga tactgctgct cttcaaaata aattagctac
     3181 taaaaaagct gaattggaaa aaactcaaaa agaattagat gcagctctta atgagttagg
     3241 ccctgatgga gatgaagaag agactccagc gccggctcct caaccagaaa aaccagctcc
     3301 agcaccaaaa ccagagcaac cagctccagc accaaaacca gagcaaccag ctccagcacc
     3361 aaaaccagag caaccagctc cagcaccaaa accagagcaa ccaactccag caccaaaacc
     3421 agagcaacca actccagcac caaaaccaga gcaaccaact cctgcaccaa aaacaggctg
     3481 gaaacaagaa aacggtatgt ggtacttcta caatactgat ggttcaatgg caacaggttg
     3541 gctacaaaac aacggttcat ggtactacct caacagcaat ggcgctatgg caacaggttg
     3601 gctccaatac aatggttcat ggtattacct aaacgctaac ggcgctatgg caacaggttg
     3661 ggctaaagtc aacggttcat ggtactacct caacgctaat ggtgctatgg cgacaggatg
     3721 gctccaatac aacgattcat ggtattacct aaacgctagc ggcgctatgg caacaggttg
     3781 ggctaaagtc aacggttcat ggtactacct aaacgctaac ggttcaatgg caacaggttg
     3841 gctccaatac aacggttcat ggtattacct aaacgctaac ggtgctatgg caacaggttg
     3901 ggctaaagtc aacggttcat ggtactacct caacgctaat ggtgctatgg ctacaggatg
     3961 gctccaatac aacggttcat ggtactacct aaacgctaac ggtgctatgg caacaggttg
     4021 ggctaaagtc aacggttcat ggtactacct aaacgctaac ggttcaatgg caacaggttg
     4081 ggtgaaagat ggagatacct ggtactatct tgaagcatca ggtgctatga aagcaagcca
     4141 atggttcaaa gtatcagata aatggtacta tgtcaatggc ttaggtgccc ttgcagtcaa
     4201 cacaactgta gacgggtata ctgtcaatga aaacggtgaa tgggtttaag ccgattaaat
     4261 taaatcatgt taagaacatt tgacatttta attttgaagc aaagataagg ttcgattgaa
     4321 tagatttatg ttcgtattct ttaggtacct gtcttatgat ttcaggaaat gtcattaaaa
     4381 aaacgactcc ttttctctaa cttgaaaaat agattagaga aaatgggttg ttttatttat
     4441 tatagttatt tgaatgaaga taagaagaag gtatactcac gtcattcaca taatctgtat
     4501 attgactata agttttaaaa aacaattttt aagctcttcc ttgtcttctc tagccaagcg
     4561 tgttataatg aatactgctc aagcgacctt caatcgtgaa gcacacacga ccttcaatcg
     4621 tgaataaacg aatagatggg agacttacca tgagtgataa ctctaaaaca cgtgttgtcg
     4681 tggggatgag tggtggtgtt gattcgtcgg tgacggctct tttgctcaag gagcagggct
     4741 acgatgtgat cggtatcttc atgaagaact gggatgacac agatgaaaac ggcgtctgta
     4801 cggcgaccga agattacaag gatgtggttg cggtggcaga ccagattggc attccctact
     4861 actctgtcaa ttttgaaaaa gagtactggg accgcgtttt tgagtatttc ctagcggaat
     4921 accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa caaggaaatc aagttcaagg
     4981 cctttttgga ctatgccata accttggggg cagactatgt agcgactggg cattatgctc
     5041 gagtggcgcg tgatgaggac ggcactgttc acatgcttcg tggcgtggac aatggcaagg
     5101 accagaccta tttcctcagc caactttcgc aagaacaact tcaaaaaacc atgttcccac
     5161 taggacattt ggaaaagcct gaagttcgaa aactagcaga agaagcaggc ctttcgactg
     5221 ctaagaagaa agactcgaca gggatttgct ttatcggaga aaagaacttt aaaaactttc
     5281 tcagcaacta cctgccagct cagcctggtc gcatgatgac tgtggatggt cgcgatatgg
     5341 gcgagcatgc aggtattatg tactatacaa tcggtcagcg tggcggactc ggtatcggtg
     5401 ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg aaaagatcta agcaagaata
     5461 ttctctatgt aggacaagga ttctaccatg attcgctcat gtcaactagc ctagaagcca
     5521 gtcaagtcca ctttactcgt gaaatgccag aagagtttac gctagaatgt acggctaaat
     5581 tccgttaccg tcagcctgac tctaaggtga ccgtccatgt caaaggagac aaggcagagg
     5641 tcatctttgc ggaaccacaa cgcgcgatta caccaggaca ggcagttgtc ttttacgatg
     5701 gcgaagagtg tctaggtggc ggtttgattg acaatgctta ccgcgatgga caagtttgtc
     5761 agtacattta gattgacaaa ttttctcaat ttgctacaat aataacagca atagaaatga
     5821 tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc acttctttgt agttttgact
     5881 gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa caagactttc ggacaaaagt
     5941 agacaatacg gtttttggtg ttcgggcgac agctttgatt cttcaaaatc gcaagcttct
     6001 agtcaccaaa gacaagggca agtattacat tatcggcggt gcgattcaag ttaacgaaaa
     6061 aacggaagac gcggtagtcc gtgaagtgaa ggaagaactg ggtgttaaat ctcaagctgg
     6121 gcagctagct tttgtggttg aaaatcgttt tgaagtggac ggcgtttcct atcacaatat
     6181 cgagtttcat tatctggtgg atttgcttga agatgccccg ttgaccatgc aggaagatga
     6241 gaaaaggcag ccctgtgagt ggattgactt ggataagctc cagaatatcc agctagttcc
     6301 agccttttta aaaacagccc taccagattg ggaaggccaa ctaagacaca ttcatcttga
     6361 ggaataggag agaaacatga cttatcattt tactgaagaa tacgatatta ttgtaattgg
     6421 tgcgggacac gctggggttg aggcttcctt ggccgctagc cgtatgggct gtaaggtcct
     6481 gcttgcgact attaacattg aaatgctggc tttcatgcct tgtaatccct ctatcggtgg
     6541 ttctgccaag gggattgtcg tgcgtgaagt cgatgccctc ggtggcgaga tggccaaaac
     6601 cattgacaag acttacatcc agatgaagat gctaaacaca gggaaggggc cagctgtccg
     6661 tgctcttcgt gcgcaggctg acaaggaact ttactctaag gagatgcgca agacagttga
     6721 aaatcaagaa aatctgaccc ttcgtcaaac catgattgat gagattttgg tggaagatgg
     6781 caaggttgtc ggtgtgcgta cagccaccca tcaagaatat gttgctaagg ctgttattgt
     6841 gacgacaggg actgctctcc gtggggaaat tatcatcgga gacctcaagt actcatcagg
     6901 tcctaaccac agcttggctt ctattaacct agctgacaat ctcaaggaac tgggcctcga
     6961 aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag gcttcttcta tcaactacga
     7021 cgtgacagaa atccaaccag gagatgaagc gcctaatcac ttctcataca cttcacgtga
     7081 tgaggattat gtcaaggacc aagtgccatg ctggttgacc tacaccaatg gtaccagtca
     7141 tgaaattatc caaaacaacc tccaccgtgc gcctatgttt acaggtgtgg tcaagggagt
     7201 ggggcctcgt tactgtccgt cgattgaaga caagattgtg cgctttgcgg acaaggaacg
     7261 tcaccaactc ttccttgagc cagaagggcg caatactgag gaagtctatg ttcaaggact
     7321 ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt cattccatca aaggtttgga
     7381 aaatgcagag atgatgcgga caggttatgc tattgagtat gatatggtct tgcctcatca
     7441 gttgcgtgcg actctggaaa ccaagaaaat ctcaggtctc ttcactgctg gtcagacaaa
     7501 tggaacatca ggttatgaag aggcagcagg ccaagggatt atcgcgggta tcaatgcggc
     7561 tctgaaaatc caaggcaagc ctgagttgat tttgaagcgc agtgacggtt atatcggggt
     7621 gatgatcgac gacttggtga ccaagggaac cattgaaccc taccgtctct tgacaagtcg
     7681 tgctgaatac cgtctcattc ttcgtcatga caatgctgat atgcgcttga ctgagatggg
     7741 acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt tttgaaatca agaaaaatca
     7801 atttgataat gagatgaaac gcctagacag tatcaaactc aagccagtca aggaaaccaa
     7861 tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat gcggtgacag ccaaagaatt
     7921 ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc ttcatcggac cagctgcaga
     7981 agacttggat gacaagatta tcgaattgat tgaaacagaa atcaagtacg aaggctatat
     8041 ttccaaagcc atggatcagg ttgccaagat gaaacgtatg gaagaaaaac gcattccagc
     8101 caatatcgac tgggatgaca tcgattctat tgcgacggaa gctcgtcaga agttcaaact
     8161 catcaatcca gaaaccatcg gccaagccag ccgtatttcg ggagtaaacc cagcagatat
     8221 ttctattttg atggtgtatc tggaaggtaa aaatcgtagt atttctaaaa ctcttcaaaa
     8281 atcaaaatga tacgtcgtcg gcttcttacg aatgagttca aagcttggct ttgattcatc
     8341 tccaacctcc catagttccc cgaactatgg gagctaactc aagttatgct ttcgcttagc
     8401 cttctagtct gattttcatt gagttttaat ctcaaaagaa agcatagaga aaaacagctc
     8461 cgaagacggg gctgttttgt tgatttgtac tcaatgaaaa tcaaagagca aactaggaag
     8521 ctagccgtag gttgctcaaa gcactgcttt gaggttgtgg atagaactga cgaagtcagt
     8581 aacatatata cggcaaggcg aagctgacgt ggtttgaaga gtattattct tcatctggtg
     8641 tgaggatcat cgggatgatg atcggttcac gttcggtatt ttcatagagg aaggggcgaa
     8701 tagcgttgac aatggcacca ttgacagatt gcacgctagc atccttattt ttcagtgcga
     8761 tacgaatggc attgaagagg atacgctggc tttggcgaat caagtcgcca gactctctca
     8821 tgtagacaaa gcctcggctg aggatgtctg gaccagatag aatcatctgc gatttgaagt
     8881 caacagttgc gactgccaga acgacaccgt cttcagatag atcgcgacga tctttgagga
     8941 cagctgcgcc aatttcaccg atacgatttc catcgacata gatatcttgg gcgttgaaat
     9001 gacctgcgat acgagctgag tcagcagtaa gggcaagcac atcgccattg ctcatgataa
     9061 agatattgtc cttctcaaca ccagtatcca ctgctagtcc agcgtggact ttttgcatgc
     9121 ggtattcacc gtggacaggc atgaagtatt ttggcttaat caagcagagc atgagttttt
     9181 gctcttgctg accaccgtgt ccagatgtat ggatattgtt cactttaccg tggataactt
     9241 cgacaccagc ttcagaaatg atgttaatca gcttgttgac gctagtagtg tttccaggga
     9301 tgggactaga agagaagata acggtatcac ctggttgtaa ttgtacttga cggtgggttc
     9361 cgttggcgat acgagagagg gctgccatag gctcaccctg actacctgta cagaggataa
     9421 gaacttctcc tgcaggataa tctttgattt catttggctc gataaaggtt cccttaggag
     9481 ctttgatgta gccaagatcg attccgttga caatggcctt ttccatagaa cgaccaaaga
     9541 ccgcaatttt gcgtccagtc ttaacagcag cttctgttgc ctgctggaga cggaagatat
     9601 ttgaggcaaa ggatgcaaag atgatacgtc cttcaatacc ttggataatc ttcataatgg
     9661 actgaccaac gactttttca gagttggtaa aggttggtac ttccgcattt gtcgagtcag
     9721 acaggagaca gagcacgcct tcttcaccaa gcgcagccat acgatgcaag tccgcaggtt
     9781 ctccaactgg agtaaagtcg aacttaaagt cacccgtaca gacgattttc ccttgaggag
     9841 tatgaatgac aatccccaaa ggctctggaa tagagtgagt cgttctaaag aaagttgcct
     9901 tgagattttt aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg gcgttgcgca
     9961 agaggccgtg ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt ccagcataaa
    10021 tagggacatt tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc tcgtgtccgt
    10081 gtgtgattaa aacagccttg acgcggtcga tattgtctac gatgtaagag tagtcaggaa
    10141 tgacatagtc gataccaagc aagtcatctt ctgggaattt aatcccagca tcgacgatga
    10201 taatctcgcc ttggtattca attccgtaag tgtttttccc gatttctcct agaccaccga
    10261 tggcaaaaac gccgacttct tcaggtttaa gagtgtaggc catattagaa ctccgtaatt
    10321 tcgaaggcgc cagtttcttt ttcgtaatcg agcaatttgt cagacaagag ttcgatatat
    10381 tcgatattgt actctgggcg attttcttcg acaagttggc gagcagtgat acggccctca
    10441 agttctgagc tggcatcgat gtctaggtaa agcgcgcgtg ttgtttcacg gcgtgggcta
    10501 cgttcttttg tttcttgata aaaaactttg taaatcatat agttcctttc tatttacagg
    10561 tcagcttatt ccaaactttt agcagatatt tgcttgattt cattccattt tgtgtctaga
    10621 ttgaccttga ttcgttacaa ttatttcaat tataccacaa aatgaaaaat tagtaaaaga
    10681 cggaaacgag aaagttaggg gacgatttct tcggtaagcg tttgcgaaaa taaagaaaat
    10741 gtattatgtt gataatggaa gtgaaattta ttgcttgtca taattccata tagattttaa
    10801 gtcaaggagg ttgccctatg tataacttat ttgcttttat ggaggaaatc ttctatttgc
    10861 caaactggaa cgatgacaaa ccaagttctt gaggaaaatt acctttgtga ctctctactc
    10921 gctttttctc ctcctcgtga cctatgtcgt ttggtttgta ttttactttg gattaaatgc
    10981 ttagatgccc tgcttatgct ggggtttttt gtttggggat gtagagatag tagatttacg
    11041 acacgaaaat tccaccccat ctatcaagat gaggtggtct ttgcattttt ctaacatgac
    11101 atgacaatgg aattttcaaa gcttattatt tttatgggag tcattgttct gctcaaaaat
    11161 agagttgaag cataaaaata aaacaataca aacagataaa tcgaatggta aagggatagt
    11221 agtaatgttc aaatcattga gaatgctgag caaactgtaa gagatgaaga caataaaatt
    11281 tatccttcta aaaatgtgtt tcttatctag catgacttac caaacttttt acgaaggtct
    11341 tttccaatgg tataacctgc cataaataaa gcgataccaa cacctacacc aaagccaccc
    11401 ttgatttctt gcaattcctt attagaaatt tcacgatagt tgttcaaatt tttcatcata
    11461 ttaatctcct cctaaaaaat aaccagctgc caaaccagaa cctataagtg ctgttccacc
    11521 tgcaatagcc ttccaagata ctgctactcc aaagataacc aagggagcga gaccgccatc
    11581 aatatctgat aattcatttt cagataagga taaaaattga ttgtcaagaa tgtcaaaatt
    11641 tgtcattgaa ctttcctcct tttttcttgt tcacttttat attcggaaaa gaaaaggaaa
    11701 agtgagcatt tttttaaatt atttttagtt aagtgagggc actcttgtct gggggcaaag
    11761 aaaattccca tgtgccagct tttgtagtat aatagtaagc agacaaaaag ataggaatgt
    11821 gttatgaaag tattagcttt tgatacgtcc agcaaggctc tttctctcgc tattttagag
    11881 gataagcagg ttcttgccga gacgacgatt aatattaaga aaaatcacag tattactctt
    11941 atgcctgcca tagatttttt gatggcaagt ttggattgga cacccaagga tttggaccga
    12001 atcgtggtag ctgaagggcc gggtagctat acaggcttgc gaattgcggt agcaactgct
    12061 aagaccttag ctcacaccct gaacatcgag ttagttggta tgtctagcct cttggctctg
    12121 gtgccatatc aacaagaagg cttgttcgtt cctttgatgg atgcgcgtcg caacaatgtt
    12181 tatgcaggat tttatgaaaa tgctaaacct gtcatgccag aagcgcacct atcttttgaa
    12241 gaggtgctag aaaaagtcaa gggtgctagt caggtaacct ttgtcggaga agttggcccc
    12301 tttgttgagc agattcaaaa acacttgcca aggactgatt acaaagaaac cttgcctaat
    12361 gcagccaatc ttgctctttt ggcctgggac aaggaagcag actccttgca tgattttgtg
    12421 ccaaattacc tcaaacgtgt tgaggctgag gaaaactggc tcaagaacca taccgagtct
    12481 ggtgagtcct acattaaacg cctatgatag aaatcaagcg aattcaacaa cagcctgacc
    12541 tagctcaagc catctacgct gttatggcag ctgtttacct agtcagttct tggactctgg
    12601 agcaaatcca agcagatctg tcccaagacc agacttggta tgcattggct tatgatgggg
    12661 cagaagtgat tggatttcta gctgtgcagg agaatctttt tgaagcagaa gtcctgcaaa
    12721 tcgctgtcaa aggagcttat cagggtcagg gaattgcgtc agccttgttt gctcaattgc
    12781 cgacagataa ggagattttc ctcgaagtca gaaagtcaaa tcaacgagcg caagcatttt
    12841 acaagaaaga aaaaatggcg gtcatcgctg agcgaaaggc ctactaccat gacccagtcg
    12901 aggacgccat tatcatgaag agagaaatag atgaaggata gatatatttt agcatttgag
    12961 acatcctgtg atgagaccag tgtcgccgtc ttgaaaaacg acgatgagct cttgtccaat
    13021 gtcattgcta gtcaaattga gagtcacaaa cgttttggtg gcgtagtgcc cgaagtagcc
    13081 agtcgtcacc atgtcgaggt cattacagcc tgtatcgagg aggcattggc agaagcaggg
    13141 attaccgaag aggacgtgac agctgttgcg gttacctacg gaccaggctt ggtcggagcc
    13201 ttgctagttg gtttgtcagc tgccaaggcc tttgcttggg ctcacggact tccactgatt
    13261 cctgttaatc acatggctgg gcacctcatg gcagctcaga gtgtgaagcc tttggagttt
    13321 cccttgctag cccttttagt cagtggtggg cacacagagt tggtctatgt ttctgaggct
    13381 ggcgattaca agattgttgg ggagacacga gacgatgcag ttggggaggc ttatgacaag
    13441 gtcggtcgtg tcatgggctt gacctatcct gcaggtcgtg agattgacga gctggctcat
    13501 caggggcagg atatttatga tttcccccgt gccatgatta aggaagataa tctggagttc
    13561 tccttctcag gtttgaaatc tgcctttatc aatcttcatc acaatgccga gcaaaaggga
    13621 gaaagcctgt ctacagaaga tttgtgtgct tccttccaag cagcagttat ggacattctc
    13681 atggcaaaaa ccaagaaggc tttggagaaa tatcctgtta aaaccctagt tgtggcaggt
    13741 ggtgtggcag ccaataaagg tctcagagaa cgcctagcag ccgaaatcac agatgtcaag
    13801 gttatcatcc cccctctgcg actctgcgga gacaatgcag gtatgattgc ctatgccagc
    13861 gtcagcgagt ggaacaaaga aaacttcgca ggctgggacc tcaatgccaa accaagtctt
    13921 gcctttgata ccatggaata aagagtggct ctttgtcaag tgtagtgggt aggcgaaaag
    13981 ctgcaatctg gagagtacga aattcgtact ctcttttttg atgttcaaag tcatcaaaat
    14041 ccgtttttta aaattgttaa agttccggaa accaaaggct ttgcgcttga taatcttaat
    14101 caaattattg gtcgcttcaa gtttagcgtt ggaataaggc agcttaagtg cgttgataat
    14161 cttatctcta tcccctaaaa aagtccaaaa gaccgtttga aaaagcggat gaaccgttgg
    14221 cagttcttgc tcaatcaatc caaagaaatg gtcggcattc ttctcttgaa aatgaaagag
    14281 caggagttga tagagttcgt agtgaacctg taatccctca gagtaagaaa gcagcttatc
    14341 tcgtacttcc cgatgggtta agtgcattcg aaacgtgggc tgatagaatc gtttctgaca
    14401 gagcttgaag ctatcctggt gtagtaaatc aatccagtgg actgatttag cccgagccta
    14461 gaaacgaaaa agcgagggtt ccaaaagtgc ttgagcgccc gatactccaa ggattttcgg
    14521 tcaaattggt tcatgatttg gattcgtact cggttcatag ctcggctcag atgttggaca
    14581 atgtggaagc ggtcaagaac aatcttcgcc tttggaaaga gttgcttagc gagccgataa
    14641 taagggctgt acatgtccat ggtgatgacc tcgaccagct cccgaacctc tctctgatag
    14701 cgttggaaat ggtttcgaat caccgcatga gttcgcccgt ctaaaattgc gaggatggat
    14761 ttggactcaa aatcttgggc aatgaagctc attttgctct ttttgaagct atactcatcc
    14821 caactcataa cttttggcaa cttggtccaa tcggtttcaa acttaaattc cctcagtttt
    14881 cggatgacgg tggaagttga gaccgccaat ctgtgggcaa tctcagtcat agactgcttt
    14941 tcaaggagga gctgagcgat tttttgtctc accatgttgg aaatctggca atttttcttg
    15001 acaatggatg tttgagaaac gaccacctta aggcaattct tgcactgaaa gcggcgcttt
    15061 ttgagatgca gtaccgtggg taaaccctga cagtcgagaa gcggaatttt agaggctttc
    15121 tggaagtcgt atttgatcat cttcccttga caatgaggac aaggaggagc gggggaatcc
    15181 aactttgcct gaacgactag atgggtttgg tatttcagaa cagacaagat tttgatattt
    15241 ttgtcttcca atccgagcaa atctgtggta ttcttaagtt gttccatatg acccctttct
    15301 aatgagttgg ttagacgctt ttcattatag gtcatatggg gctttttttc tacaagaaac
    15361 gaccctataa ttcctggggt gggattaccc actacagaaa ttatagagcc aaaacattcc
    15421 aaagtcttgt ctgactctgg aatgttttct gttcacagtt gttttgtaac tgcctaataa
    15481 gtaaccgcgc tataacgagg tatctaccga gattgtcaat tctccagtat actgttataa
    15541 aaaacaaaac tggaggattt ttttatgtcc ccccatcgtc ccatcgactg ttccgtagct
    15601 gcccaataac ggagtatagt cgtctgaaac aagaatagga caaaagtgcc tcaagaaaag
    15661 catcgtaatt tgacaatatt attctgagat gtttttgatg taagcccata ttttctcaat
    15721 gggattatac tcgggtgaat aggtaggaag tggtaacagt ctatgccctg ctccttacac
    15781 atgttcattc tgtgaaacct tgcattgtcc ataatgataa gggatggtgt atctaaagta
    15841 ggtagtaaga atttttggaa gcaagcttcg aaaaagtcac tcgtcatcgt atctttatag
    15901 gtcatcggag ccatagtata ttgaaacaaa aaagggtatt agtacttcta taacagtatc
    15961 gcaatctctg gtgtgcgttg tctcgatctt aattcaaata attatatctt cttccagaga
    16021 gactaactgc ctgctcaagg atcaatcata ttcgagataa aaataggtct caacccctgt
    16081 ctcataaata taaataggag tcaggtagct taagtgattc aattctttaa ggaaccgatt
    16141 tactttttct gggtcttgtt cgcagtaggt acggctcttt tttagactat atcccatagc
    16201 ctttagagcg taatgaatag ttgttggagg acagttgaat tcagcagcta tttcagtcaa
    16261 ataagcatcg ggatgagttt caatatagat ctttaattta tctctatcaa ctttttcaat
    16321 taatagtgga agttttcttg aagttagtgt attgaaacgt ttttatacag tgctaaaatt
    16381 tgaagtgtag tgcagatgta tttgtaatag tattatagca attctttgga gaaggtcgag
    16441 acaagagaaa ataggtatct ttgagatata agcatgattt actataaaat tattgagtta
    16501 ttcataaaaa tttctctatc aaatatcata atgcgtcgtt taaggagatg gttacaatgc
    16561 aatatagttg tggaaaaata aatattaata tccctgatgg atatggagac attaaggata
    16621 tagtgttttc ggctcatatt atagttaggt ataataatgg acattgtgga ggtatagatc
    16681 cgcatataat tggactttgt aaaaagcaga tcagaaggat gtctctatat cctattttga
    16741 taatcgtatc tagagattca aaggttattg atgattataa aaatttagat attgcctatg
    16801 ttgattgtac tcaatgttca aataattttg aaactgcctt acacgttaaa aatattttaa
    16861 aattattaaa aatccaactt atacactgtc atggctactc aactaattat tttttatata
    16921 tgttaaaaaa acttgacaag aatggatttg gaaaggttaa gacagtgata acatgccatg
    16981 gatgggttga atataactta aaaaagaaat tcttgactta ttttgatt
//
LOCUS       DAVMHG010000037        16763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMHG010000037 DAVMHG010000000
VERSION     DAVMHG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.037"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..287)
                     /locus_tag="VYR08_000941"
     CDS             complement(<1..287)
                     /locus_tag="VYR08_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV3466486.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFD"
     gene            complement(274..1044)
                     /locus_tag="VYR08_000942"
     CDS             complement(274..1044)
                     /locus_tag="VYR08_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV3466487.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(1070..1924)
                     /locus_tag="VYR08_000943"
     CDS             complement(1070..1924)
                     /locus_tag="VYR08_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV3466488.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(2081..2485)
                     /locus_tag="VYR08_000944"
     CDS             complement(2081..2485)
                     /locus_tag="VYR08_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV3466489.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(2555..4036)
                     /locus_tag="VYR08_000945"
     CDS             complement(2555..4036)
                     /locus_tag="VYR08_000945"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV3466490.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFAHVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(4055..5233)
                     /locus_tag="VYR08_000946"
     CDS             complement(4055..5233)
                     /locus_tag="VYR08_000946"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV3466491.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            5398..6405
                     /gene="galR"
                     /locus_tag="VYR08_000947"
     CDS             5398..6405
                     /gene="galR"
                     /locus_tag="VYR08_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV3466492.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTIAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGSYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRVGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(6451..7488)
                     /locus_tag="VYR08_000948"
     CDS             complement(6451..7488)
                     /locus_tag="VYR08_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3466493.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(7489..7842)
                     /locus_tag="VYR08_000949"
     CDS             complement(7489..7842)
                     /locus_tag="VYR08_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV3466494.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(8086..8976)
                     /locus_tag="VYR08_000950"
     CDS             complement(8086..8976)
                     /locus_tag="VYR08_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV3466495.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGIFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            9109..9645
                     /locus_tag="VYR08_000951"
     CDS             9109..9645
                     /locus_tag="VYR08_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV3466496.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            complement(9811..10617)
                     /locus_tag="VYR08_000952"
     CDS             complement(9811..10617)
                     /locus_tag="VYR08_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV3466497.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSETMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(10961..12481)
                     /locus_tag="VYR08_000953"
     CDS             complement(10961..12481)
                     /locus_tag="VYR08_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV3466498.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENVIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(12474..13202)
                     /locus_tag="VYR08_000954"
     CDS             complement(12474..13202)
                     /locus_tag="VYR08_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466499.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(13217..13981)
                     /locus_tag="VYR08_000955"
     CDS             complement(13217..13981)
                     /locus_tag="VYR08_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV3466500.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(13985..14428)
                     /locus_tag="VYR08_000956"
     CDS             complement(13985..14428)
                     /locus_tag="VYR08_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3466501.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(15110..15406)
                     /locus_tag="VYR08_000957"
     CDS             complement(15110..15406)
                     /locus_tag="VYR08_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3466502.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(15428..16492)
                     /gene="pepA"
                     /locus_tag="VYR08_000958"
     CDS             complement(15428..16492)
                     /gene="pepA"
                     /locus_tag="VYR08_000958"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV3466503.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         4997 a         3936 c         2875 g         4955 t
ORIGIN      
        1 aaatcaaaat agaaatcttt tatcggatcc ttaacaataa tcgtatctct caaacgttta
       61 gcaataagta gaggcacaac tttcaaaatt tctggataat tttgatataa gtctaaaaaa
      121 tcctcttgta tatttttact accaataaga ctattcataa tatttagagc aacttttata
      181 ctagatacat ttttataaac cttaggaaaa tctgtataat aagtccagtc agctactgtg
      241 tcggtcatcg ttgacaacca ctcatcaaaa tttctagttt gtttcatttt ctgcctccaa
      301 cctcttcctt gcaattttta aatattcttt ttcagcatcg atacctataa atcttctacc
      361 taaccgcttc gcaacaacac ccgtagtgcc actaccaaca aatgggtcta gaatatagtc
      421 accctcttta gtagaggcta aaataatgcg ttctaacaaa tactctggtt tttgagttgg
      481 atgtttccca gcccattttt caactttctt tgttaaagaa ccggtccaga catctttcat
      541 ttgttttcca tcattcaatt ctttcattaa atcataattg tagtaatgac gagctttttt
      601 atcatttttt ctggcccata aaatggtttc agtagaatgg gtaaaataac gacaagataa
      661 attgggggca gggtttgttt tctgccaagt aatattattc agaattttaa aaccttcttg
      721 ttctaatgcc attccaactg agtatatgtt gtgcaaacta cctgaaatcc ataccgtccc
      781 attaggcttc agaacttctt ttgctaggcg aatccattta cgattaaact catgtttttc
      841 ttcgaatgaa gaaattttat cccaatctcc tttatcaaca gaaacaactt gtcccccaga
      901 attagatatt ccaccattac ttaaaaaata aggtggatca gcaaaaatca tatccatact
      961 ttctggtttc atttttgata agaacttgaa cgtatctgaa tgaacaagaa tcatcttatt
     1021 tttattgtag tatggttttg tcataagaac acctccatac ttatattcgt tatttttcat
     1081 aatttgtgac tataatttca gaaatttttc ctcgacttga agattttgct ccattagttc
     1141 gggtagcttc aacataatgt atattaaaat ccttatacaa ctcctctact aaagcactag
     1201 aagaatttga taacataaca taagctcctg tatcactcaa tctcttaaag gcatctctta
     1261 atcttacttg atctgcaaaa gagaatccct catgagtata agacgtaaat gcactcgtct
     1321 cagacaatgg aatatatgga gggtcaaaat acacaaaatc tcctgttcga acatctacaa
     1381 ttgccttttc aaaatctccc actttaattt ctagttgatt gttatttaga taaactgaaa
     1441 tagcagatat caattcctca tcaacaattt taggattctt ataacgtcca tatggaacat
     1501 taaattggtt cttagaattc acacgatata gaccattaaa gttcactctc aacatatata
     1561 gaatacgtgc agctctttgt acttcggaca tcatatctat tctttcatca cgatctgcag
     1621 aacgtaaatc taaataatat tcttttgaat tatattcctg atgaactttc aaaatttcaa
     1681 tcaattcttg aggattgtcc ttaatttgtt gatagcaatt tattagttca gcgttaaaat
     1741 cattaataac tgcatcttta ggagccaaat caaaaaataa agctccacct ccaacaaaag
     1801 gttcgaaata cctgttatag gttttaggca ttaattctct aataacaggc aataattgtc
     1861 ttttaccacc tgtccatttc gtgaacggtt gtaaagtaac tttctttatt tcttttatct
     1921 tcatgctttt aattatacca tagaaattaa aacacaactc ttgtattttc taccacatta
     1981 ctaaattttc tattttagaa ttaaatcata tcaatatcaa aaaatgagta aggcaaatct
     2041 tataaattcc aaaattcacc tcactctatt aaattactct ttatatcctt acccgtcttt
     2101 cctcagacct ttggcctgtg acaaacatgg taaaggttgc tttacagaca tttctgccct
     2161 cttgattggt gatatccaca tccatcacac aggttgtacg gccttgatgg acacattctc
     2221 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
     2281 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagcg
     2341 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
     2401 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
     2461 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaacga cactatgata
     2521 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtgaaatt ccgacatgtt
     2581 ccacaaagcg cataaaggct gtctgccctt gttctgtctg cttgtagact cctgcatcct
     2641 caagtacatg cgcaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
     2701 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
     2761 caactgtaac agcttctcct acaagatagc tagcgacttg ctctacttct tctttcagac
     2821 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
     2881 gttggacatc cttgtgggga tgatagatgc catcaggatg ctctgctgaa gtctgattgt
     2941 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
     3001 tgtgatgcgg tgtcccgtct gtctctgcta aaatctgcac actagaatct gaatactgac
     3061 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
     3121 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
     3181 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
     3241 aatgatcgtg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
     3301 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
     3361 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
     3421 aatactggaa cccccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
     3481 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
     3541 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
     3601 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taagcgatat
     3661 ttttagcaat ggccttgagt ttgatgtagt catttttctg actgagttga taaaaatcct
     3721 ctatcgcttg ctcaggagag taggcatagg cttcccagaa gtcacgattg acctgactcg
     3781 gacaaggagt caccaagtcc atcagttcag taccaaggat ttcacgcgca gtctgactat
     3841 cctcaatcgt ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
     3901 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacgattgg
     3961 tcaggtagat tcgatccatt tcctcaaatg aactttcaga aatgacatgt gttacaaatt
     4021 tatctactaa ggtcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
     4081 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
     4141 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
     4201 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
     4261 gggtatctaa ttccaaacca gttacttcat aatcatgctc cagagaaacg tgtgacgcat
     4321 tcatcaaacg accaaatgtt tccaaatcac ctgcttgaag ggctgcttga gctttaaggg
     4381 tacgttggtt ttcaagcaca gcatggcgag cacgtttcaa acgattttca tctttaatta
     4441 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagggtctga atatccaagg
     4501 caacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
     4561 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
     4621 gtggcaccaa atcatactct aaggtgttgg tatcaaggta aatagcacgt tgatcagccc
     4681 ccataccaat agcaaactgg tccatgatac cagagttgac tccgataaag ttattttctg
     4741 tttgttttcc gattttaacc aaatcgagac ggtctagttt taaatcaaag agatgctctg
     4801 ccacgactcc tgtcaagagc tccaaggatg ctgaagaaga caagccagaa ccatttggga
     4861 tattcccata aacataaaaa tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga
     4921 aatgaagaac cccttttgga tagttggtcc agttgtgctc tttttcaaac ttgaggtcag
     4981 cgagaggcac ttcaattatg cccttgtctt caaagttagc tgagtagaaa cgtaaaactt
     5041 ggtcgtcacg cttgcgagct gcaccataag ttcccaagga aatagcagct ggaaaaacgt
     5101 gcccaccgtt gtagtctgtg tgttcaccaa tcaaattaat acggcctggt gaaaagaagg
     5161 tttggtctgc ttcttgacca aaaacagcaa gaaagtcttt gcgaaggact tcagtagtaa
     5221 gatgttgtgc catatgaatt ctcctttgac tgtccgttaa gccatcttaa cgtgtaatcg
     5281 ttttctttct attgtatcca aggaatttcc caaggtcaat actttttact aaaattttag
     5341 taaaatatca gtaaaaagaa gaaaaatatg atatactaac ctaatcaagg aggatttatg
     5401 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc
     5461 ctcaatcgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccatt
     5521 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccacaa acccaagcaa
     5581 aagattgcca ttatccaatg ggtcagcgaa caaggggagc tggacgacct ctactactac
     5641 cagattcgcc taggaataga aaaaagagcc caagagttgg actatgatat cttgcgctat
     5701 tttaatgacc atccttttac cttaagcgag gaagtgattg ggattctctg catcggaaaa
     5761 tttagccgag ctcagatttc tgcctttgaa gaataccaaa aacccctggt ctttctagac
     5821 agtgatacac tttccctggg gcatacttgt attattacgg acttttacac tgccataaaa
     5881 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacaggcctt
     5941 gaagaaacaa ctgaccaaga agaaatcatt gaggataagc ggctggaaaa tttcagaaac
     6001 tacagtcaag caaaaggaag ctatcatgat gaactggtct ttcaaggaaa ctttactgcc
     6061 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
     6121 gcatttttcg cagccagcga tagtttagcc atcggtgccc ttcgtgcact ccaagaagct
     6181 ggaatcagcc tgccagatcg cgtcagcctc atttcattca acgacaccag tctgaccaaa
     6241 caggtctatc ctcccctctc tagtattaca gtttatactg aagaaatggg gcgagtaggt
     6301 atggatattc ttaacaagga agtcctccac ggtcgcaaaa ttcctagcct gaccatgctg
     6361 ggaaccagac tgacattaag agaaagtact ctaaatcaag aataggataa cataaaaaac
     6421 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaattgt
     6481 cttacgttca tccatatctt tataggcttg gtcgatatct tccagtttat aacttgaagt
     6541 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
     6601 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
     6661 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
     6721 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcatctgc
     6781 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
     6841 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
     6901 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
     6961 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
     7021 tgtcggcatg acatctgcaa gagtcaaaag ggacttgagc atcccttctg tatagtcaga
     7081 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
     7141 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
     7201 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
     7261 tttcaccgtc gtaatggctt ccccagcttc ttcaacaatc ccaatcgctt cgtgtccact
     7321 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
     7381 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgttcaat
     7441 gctagcaagt ccaacctgac ctgcctttgt atatactgct gatttcattt aaaattttcc
     7501 ttccttataa agtttaattt tgagatttaa acgatttaaa gctgtctgta gctgagacaa
     7561 gcgctcctct aatttttgct tttcctcttc taaaatacca agcctctcct ctctcgtttc
     7621 atctcccttt tggtagagcg acatatagtc aactaaacta tctacagaga cacccgccga
     7681 acgaaaacac ttaataaatt ccagcgcttc gatatcctga tcttgaaaat cacgaatccc
     7741 agtagcagta cgagtaatcg gtggcacaag accaacccgt tcataatacc gaatcgtatc
     7801 cgctgaaatt cccaacaaat cactggcaga tttaatattc acacgctaac ctcctctcat
     7861 ttcattcact gaattcacgc tattctaact cacttatctt attatataac tttgagttga
     7921 ctccaagtca agcattttgt atctaatcga aaaattttta tcattaacaa tcatatatct
     7981 ggagtttata caatgctctt ctcttaccaa tttttgaaat atttaaataa taaagaaatt
     8041 actctattcc aaataagata gtacccaagt atttttcact tttttctaat gttgatgctc
     8101 ataactccgt tccaagtcac acacctttcg cttatgggtt tggtgagttt ctaggtcagc
     8161 atcaatttca atggtaatat tttgaaaacc acaatctttt aggaaaattc gaatagactc
     8221 tttacaagtt tccatatgtt ccatttcttt tagacaaaca tggacaatgg catttttttc
     8281 caaagcatcc atagtccaga gattaagctg attaaggctg accacattgt ccaatcgctc
     8341 caggccactc tttacttgct tgatatcaag accttctggc acagcatcca agaaaatctt
     8401 gagtgtagac caaaaacgtg gaagggcttt tgaaagaata aagaaagaaa tgacaaggga
     8461 caaaagagga tctaggatat accagtccgt aaatcgaaga acaatcgcca tcaggataac
     8521 agctacccac cctagcgtat cttccagaaa atgcagactc agaatagact cattctttgt
     8581 ctttccctta ccaaccacca gactcgctaa cagattgata gtaatcgcaa taattcctaa
     8641 ccagagaatc ccctcatcat tgactggttg cggatgcaaa atcttcgtga cattttccaa
     8701 aatgactaga acagagcccg ttacgagaat cacagctgtt accaaggctc ctagcaggct
     8761 aaaccgctta tagcccaagg tgtactgatt gtcttcttca cgattggaga ttgtttctaa
     8821 aaaagctgat attccaattg caatcgcatc tcccaagtca tgcacagagt cagcaagaac
     8881 agcgctagaa ccaaatattc cacctgcaat aaactcaaca atggcataag tcaaatttaa
     8941 gaaaaaagcc acccaaacag catattttgc cttcatattt ctcattcctt tgttataata
     9001 gatttatgaa caccttgttc attatcatta tccactaaaa ctcaaagaaa ggatagaagc
     9061 aaaacgtcag ctttattcag ttctgaacaa tttgccttaa gtgtccatat gactaacatt
     9121 gaccgccgta tcagcaaaac caaaaaagcc atctatcaag cttttataca acttttgaat
     9181 gctaagggct acgaggccac tactgttcag gatatcattg atctcgcaga tgtgggaaga
     9241 tccacctttt actgtcacta tgaaagtaag gagcttcttc tggaccagct ctgccgctac
     9301 ctcttccatc atctttttga aagagagcaa gccatttcaa ccgaggacta cctcgcccac
     9361 ctctttctcc attttcataa aaaccaagac catatcacca gtctgctatt ttccaaaaat
     9421 gactacttcc tccgtcaact ccataaagag ctagaacacc atgtctattc cgtgctagct
     9481 gataatttaa aagaagccca ccctaatatg cctacttctt acctccaaca cttggtcgtg
     9541 tccaacttta tcgagacatt gacctggtgg ctcaaaaaag gtcaagattt cacaggccag
     9601 gaagttgtcc aattttatct agaccttctc attcctaaaa attgaatata gagtaaagct
     9661 cagttgtctt atttctaggt tactgagttt tttatctttt caacaacaaa agaggacccg
     9721 ccgatcctct ttttcatact ataaatcctt gattatcaac tatatctgtt ttaatcgaaa
     9781 tctcaaaaca gcactttcaa acatcttttc ctagttaagt aaatcagtat tctgcttagc
     9841 tgccttgctc cattgatacc aaccaactag actgttaatg agataaatta gatatttccc
     9901 ttgaatttgc aggctttctc cccaccagag atagattgaa aaaacattgg tagccgccca
     9961 gaatatccac tgttcacggt aaacagctgt catgaggatt tgccctaccc cattggttgc
    10021 atctgtgatt gaatcacgat agggacgatt ggcaccaata gactgataaa tgaagccaaa
    10081 ggccaaccac caaagcacac taatggaaag atactttgtc cagcccttgc cgtccagttt
    10141 acgcgcgaca aactcctgct tttccttctt aaactgtgcc tgataaatcc aaactagaag
    10201 tccaattggc tgcatgactg tgaagtaaag tgtcgtcagc acctcaccat aaaagccttt
    10261 ctgtagggcc aaaataaggt aaataacaga gttaatcaag ccaaaaagat aattacttgc
    10321 tcgaccttcc gatacaaaga ttacacagat aatccctgtc aagctacaaa tcatcccaat
    10381 ccagtcaaca atacgatgtt cgtaaaccaa ctccagccag agaggaaaac ttcctaaaac
    10441 cagcaaataa atccactggg caaaactacg atgggcaaag aggtcatccc agatagcctt
    10501 catagtttct gaaaatccta aatcagccat agccgcaacc atacgacggt aaccacctga
    10561 catttcacct agggttgttt tgatattttc aattttcttt tgcaaataag tatgcatcat
    10621 ttctcctttt gtttttaaag agccgtgtct ggatagactt tcggacgcaa cgctctatta
    10681 gataatgaac tgcctataca caagatttct aaccttagtc gacatgagct gaaacctctt
    10741 atttgttaag tagttcacaa aatattatac acctatttta tgaatagtca actgtcttta
    10801 cagtaaaatt ttagaaaatc atgaaaattt tctctttctt tccattttaa gtgacattca
    10861 gtcattctca catcaaaaag cccagacgaa attgtctgag cattctttta tctagtcgtt
    10921 taaggaagtt gagttcagta tgtttaaagt ctctgtccca tcatttcttc aacaaacctt
    10981 gttcttggag aaactccttg gctacttgct ttgctgactt gccttcaaca ccgacttggt
    11041 agttgagctg gctcatctgg ctttctgtaa tcttaccagc caatgtatta agaactcttt
    11101 ccaactctgg gtgtttcttg agaagagctt ctttcatgag tggagcccct tgataaggtg
    11161 ggaagagttg cttgtcatct tccaagacct gtaaatcata acgctccaat tccgcatcag
    11221 tcgaataggc atccgtgatt tgaatatccc ctgactgaat tgcctgataa cgaagagctg
    11281 gctgcatagt cgccacattg agattgagac catacattga ttgcaagccc ttatttccat
    11341 cttcacggtc gttaaactca agcgtaaagc ctgccttcag ctgcccttcc acttttttca
    11401 aatccgaaat ggtcttcaag ccatattctt gcgcaatctt tttcggaaca gctacagcat
    11461 aggtattttg ataagacata ggtttgagat aggctagatg atcctgcttg gcaatgccat
    11521 cacgcgccac ttgataaacc tgctctggct catgactcac cttgggtgat ggttgaagca
    11581 aactttcagt caccgtaccg gtaaattcag gataaatgtc aatatcacct tttttcagag
    11641 cttcataaag gaaacttgtc gtcccaaaat tcggtttaac agtcgcagtc atgctggtat
    11701 tttcttcaat cagcaattta tacatattag ccaaaatttc tggttctggg cctattttcc
    11761 cagcaataat cacgttttcc ttctcttttt gagccaaaag ggctggacta taagacagac
    11821 ccagtaataa agccaccaag gcaaaaccag agaaaatcgt ccgcaatttc gctttttcca
    11881 ttacttttag taggaagtta aaggcaatag ctagcactgc agaagaaagg gccccaatca
    11941 aaatcaaact ggcattatta cggtcaattc ccaacaggat aaaggaaccc agtccccctg
    12001 ccccaatcaa ggtcgccaag gtagccgtac cgataatcaa gaccgctgcc gtccgaattc
    12061 cagacatgat aacaggcatg gcaagtggaa tttcaaactt cttgagacgc tcccatctgg
    12121 tcatcccaaa ggcaatccca gcctcttgaa gactcggatc aattcccttc agcccagtaa
    12181 tagtattttg taaaatcgga aaaatcgcat aaatcactag agctgtcaat gccggtaaag
    12241 tcccaattcc catcaaggga ataaagagtc ccaacaaggc cagagacggg atggtctgga
    12301 aaatacctgc aatctgcaag acccagtcgg ccagcttctc atgatagcga agaaaaacag
    12361 ccaagggaat cgcaagcaaa atagctagta acaaggtcaa aagtgacaac tgcaaatgtt
    12421 gagatagagc tgtcaaccaa tcactaaaac gatcctgaaa agttgcaatt aaattagtca
    12481 tgaacactac ctccaaacaa gtctgctaca aagtctgttg caggcgcttt taaaattgtc
    12541 tcgggattcg ctacctggcg aatttctcca tcctgcaaga cagcaatacg gtccgccaac
    12601 ttcaaggctt catccgtatc atgggttaca aaaatcgttg tcatcccaaa ctctttatgc
    12661 aattcttttg tcagaacctg caactgtttt ctcgaaatag catccaaggc cgaaaagggt
    12721 tcatccatga ggaaaatctt gggctgacca atcatagctc ggacaatacc gacccgttgc
    12781 tgttctccac cagataattc actaggtaag cgatgcccat actcggctac tggtaaacca
    12841 accttagcca aaagctcttc tgttttcttc gtaatttctt ccttgctcca ccccttcatt
    12901 tcaggaatga gagcaatatt ttccgcaact gttagatttg gaaaaagagc aatagcctgt
    12961 aaaacataac cagtagaaag acgaagttca cgctcatcat agtctttgat gcgcttccca
    13021 tccatataaa tatttccatc agttggttcc aaaagacggt taatcatctt gagcatggtc
    13081 gtcttacctg acccagaagg ccctactaaa accataaatt ccccatcctc aatctgtaag
    13141 ttgacatctc tcaagacatc cttttctgtg tagcgcagtg ctacattttt gtattcaatc
    13201 attctttgtc ctcaatttaa aacttccctc gattagtcaa gtcttctacc ttaggcatga
    13261 cttccttatt atcccaatgc tccacaattt tcccgttctc taaacggaag atatcgtact
    13321 gggcataagc aacgccatca atctgagtct gaccatagct aaccacatag tttccttgtc
    13381 ctaagagttg gaaaacaaag tcaaaagtga cactatattc agccacatag tttttataag
    13441 cagcacttcc ttgtccaatc tcatgattat gctgaatcaa atcgtctgcc acataatcac
    13501 tccactgctc tagctcccca ttttggaaaa tttctgtcaa gaaacggcga accagctttt
    13561 tattttctgc tttcttatcc aaatccttga tttcaaaatc tccaaaaatt tgatctagtt
    13621 ggtcattttc aggtgttcga tagtagtcaa tgacatccca atgctcaaca atacgaccat
    13681 tctcatccgc acggaaagta tccgtcgtca cccattgagc ttctccacca ttcagatatt
    13741 gatgaacatg aacaaagacc agattgccat cctcaatggt gcggacaatc ttaatctgac
    13801 gctctggatg acgctcaaag aaatctgcaa agaaggctgc aaatccttct ttcccgtcag
    13861 gaacacctgt cgaatgttgg atataggtat cccctacaga ctgggcttga gcctcagcaa
    13921 ctcgtccgtc ttgaatggca tggatgtata ggttgtgagc atttttcact tgttgtgaca
    13981 tattctaaac ctcatttccc ttctctttca aattcgccaa aattctttct tgaaaacctt
    14041 caaattggtg aatttcttcc tctgaaaatc ctttgtaaaa gatagtatcc aatttctgac
    14101 tgacacgatg ccccacttct ttctgggact tgcctaactc cgttaaaact aaatacttct
    14161 tacgcttgtc ttttccacac ggactaacaa ttacaagctt ttgttcctct agctttttta
    14221 tcatagtcgt cagcgtatta ttcgcaagcc cagtcgcaag cgcgatatct gtcgcagttg
    14281 cgcagccagt ttcactattc cataaaaccg ctaaaatctt gccctgttca cccctataaa
    14341 gagcctcagg atcttgactc agtaactttt gaaaaatccg cccattcaac aaacgaatat
    14401 gatgggctag caaatgacca tctttcataa cacctccaat ttatttcgat atcgaaatga
    14461 ataaaacaat tgtaacactc atcgttctaa ctgtcaacta tttcgattta gaaataattt
    14521 ttgataatta tccacaccac catactccgg ctcaactaac ttttaacgag agtttctaaa
    14581 ctccttcgtc ctccagtcta caaaagcctt ccattcgtac tatcctatat tttatgaggg
    14641 gacacatttt tcctatcaga ccatttattt taaagataga agtaaatcat aattgcttcc
    14701 atctgttctt ttatagtata ttgaagttag actagagcac tgtatcttct aaaacattga
    14761 tagaaagcga tttgaatttc ccaatcaatt tgttcgtatt tatagcattt cgaaactgga
    14821 ataggacacc atgactgcta aaagatttct ataaattcat ttaatttcct caatcaattt
    14881 gttcatatct tatttcattc cgctataatt tcaccttacc ctatcttttt cgtagcaccc
    14941 ttcaaacagc ctatccccta ccgtttgacg attcctcact tcgctccact tccattacag
    15001 aagtttcttc actactatgg gctcggctga cttctcatga ttccttgtta ctactatttg
    15061 aacgctcacg agatagatct tacaaaaaat gctttgatcc acaatggaat caaagcattt
    15121 taaagagttc ctcatacata agcgcagaag tcgcagttcc tctgtacttg gcttcttctc
    15181 ttttgataaa gcgagccaag ttgagcaact caggtgctgg atgtttggga tttaggagca
    15241 attcacgatt gaccaggcct gagagacgaa ctgcctgcaa ttgctcattt gtagtaggca
    15301 gttttttagc agtctctagg agagcagcaa ctaaatcttc actcaaatca tgtcgagcat
    15361 gattgtaaag atcttttata aggctttcta ggtttggttc tgccatccct accacctccc
    15421 ttatggttta ataatgttta atcaaatcaa ccgttgaacg atccaatttc ttcaccaagg
    15481 cttgtaagaa agcttgcgct tctaggaagt catccattgc atagagggtt tggtgagaat
    15541 ggatataacg agcgcagaca ccgatagttg ttgatgggac accaccattt ttcagatgag
    15601 ctgcacctgc atctgttccg cctttaccac agtagtattg gtacttgata ccagcttctt
    15661 cagccgttgt caaaaggaaa tccttcatcc ctggaagaag caagtgacct ggatcataga
    15721 aacgaatcaa ggttccatct ccaatcttgc cttgaccacc gtagacatca cctgctggtg
    15781 agcaatcaac tgcgaggaag acttctgggt caaacttggt tgtagaggta tgagcgccac
    15841 gcagaccaac ttcttcttgg acgttagaac ccagatagag ttcattgccg agtttttgac
    15901 ccgataaagc ttcagctagc tcgcttacca tgaggacacc gtagcggtta tcccaagctt
    15961 ttgagatgat atttttttca ttggctgtca aaattgcaga actatctggt acaatggtat
    16021 caccaggacg gatgccaaaa ctttctgcct cagccttgtc cgcaaaacca ccatcaaaaa
    16081 cgatatcggc aatggctggc atggttggtc ccccctttcc acgagtcaaa tgcggaggaa
    16141 cagaacctga aatcacagga atttcatgac catcacgagt caagagtttg aaacgttggc
    16201 tgctaaccac catggggttc cagccaccga tttctacgac acggaaggta ccatctggct
    16261 tgatttcgct gaccataaaa ccaacttcgt ccatatgaga agcgaccaag acgcgcggtg
    16321 catccacagc ttctgaatgt ttgataccaa aaataccacc caagccatct gtcaccactt
    16381 catccacatg cggtgtcaac ttttcacgaa gataagcacg gacaggcgct tcatgacctg
    16441 agactgcagc aagttctgtt acttctttaa tttttgaaaa taatgttgtc atttcagttc
    16501 cttctttctt tcatccattt taccactttt tataggagaa ggatagtggg aaggtggatt
    16561 tctaagttag tatcttagtc ctgctctatc ttagaaaagg atagtattct cttgcatgta
    16621 gtgcaaaatc tagtaaacat tccaaaatta actcgaatat ttatttccaa acaaaaaaac
    16681 aatacaccat caaagttgtt tggatttttc atgaaattta cagaaaatag ttgacttccc
    16741 tttcttcttt ctttaaatat ata
//
LOCUS       DAVMHG010000038        15820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMHG010000038 DAVMHG010000000
VERSION     DAVMHG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.038"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            83..946
                     /locus_tag="VYR08_000959"
     CDS             83..946
                     /locus_tag="VYR08_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3466504.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(998..1585)
                     /locus_tag="VYR08_000960"
     CDS             complement(998..1585)
                     /locus_tag="VYR08_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV3466505.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            1827..2498
                     /gene="sdaAB"
                     /locus_tag="VYR08_000961"
     CDS             1827..2498
                     /gene="sdaAB"
                     /locus_tag="VYR08_000961"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV3466506.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2507..3379
                     /gene="sdaAA"
                     /locus_tag="VYR08_000962"
     CDS             2507..3379
                     /gene="sdaAA"
                     /locus_tag="VYR08_000962"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV3466507.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            3401..4033
                     /locus_tag="VYR08_000963"
     CDS             3401..4033
                     /locus_tag="VYR08_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV3466508.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            4059..4157
                     /locus_tag="VYR08_000964"
                     /pseudo
     CDS             4059..4157
                     /locus_tag="VYR08_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4380..6230)
                     /locus_tag="VYR08_000965"
     CDS             complement(4380..6230)
                     /locus_tag="VYR08_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3466509.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(6269..7429)
                     /locus_tag="VYR08_000966"
     CDS             complement(6269..7429)
                     /locus_tag="VYR08_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3466510.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            7487..8653
                     /locus_tag="VYR08_000967"
     CDS             7487..8653
                     /locus_tag="VYR08_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV3466511.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            8792..9118
                     /locus_tag="VYR08_000968"
     CDS             8792..9118
                     /locus_tag="VYR08_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV3466512.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            9105..9698
                     /locus_tag="VYR08_000969"
     CDS             9105..9698
                     /locus_tag="VYR08_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV3466513.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            9691..10602
                     /locus_tag="VYR08_000970"
     CDS             9691..10602
                     /locus_tag="VYR08_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV3466514.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            10665..11729
                     /locus_tag="VYR08_000971"
     CDS             10665..11729
                     /locus_tag="VYR08_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466515.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11907..12767)
                     /locus_tag="VYR08_000972"
     CDS             complement(11907..12767)
                     /locus_tag="VYR08_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV3466516.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEMQPILD
                     KDFKTLAQEK"
     gene            complement(13042..14028)
                     /locus_tag="VYR08_000973"
     CDS             complement(13042..14028)
                     /locus_tag="VYR08_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV3466517.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(14300..15775)
                     /locus_tag="VYR08_000974"
     CDS             complement(14300..15775)
                     /locus_tag="VYR08_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV3466518.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
BASE COUNT         4651 a         3452 c         2941 g         4776 t
ORIGIN      
        1 tttacttttt acattgctat gatacaatat ttttaacaaa attttattgt ttgttaactt
       61 ttttcacgaa aaggagaaaa tcatgcgtta tgatttcgga aaggtctata aagaaatacg
      121 tgagtcaaag gggctgaccc aagaagaggt ctgtgggggt gttctctcaa gaaccagctt
      181 atcaaaaatc gaaagtggca agacaacccc aaaatatgaa aatatggaat ttcttctccg
      241 tcaaatcaat atgagttttg aagagtttga atatatctgt caactttatc aaccaagtca
      301 acgcacagag attatgcaaa cctatctcaa tatgcgttcc attatcggga ccagcgatct
      361 tgtcaattta tttcaaaaat gccaagacta cctcaagacc caccacgatc tccctataga
      421 agaaatcaga gatatgcttg aagttgttat ttatctccgt caacacgggg caggggaact
      481 atcaaaacac gcagaacagg ttgtcaaaaa actttggaaa aaaattgaaa aacaagatac
      541 atggtatgag agtgatctaa aaatcctcaa taccattctt ttcagttttc ccattgaata
      601 cctccatctc atcactggaa aaatattgca acgcctagaa gtctataaaa actatcaaca
      661 tttatatgac ttgcgaatga caattctatt aaacctttcc acactctatc tatacaatca
      721 agataaaaac atgtgtaagc aaatctgcta cactttacta gaggacgcca agaataagaa
      781 aagctacgat aggctggcta tctgctatgt ccgtattggg atttgtacag acgattctaa
      841 acttatccaa aaagggttct cccttctgga gctaaccgag gaaacttcta tgctgtctca
      901 tctcaaaaaa gaagtagaga tctattacca agcgaaggaa agataaaaaa gccgagggat
      961 ttcctcgact ctttacatat tatttgttcg ttagttctta ataccagcca ttgttaagcc
     1021 agaagttttt agcagcagtc catgaaccgt aacgtcctgc aacgtaggca tctgctacac
     1081 gttcttggtt ttcagctgag tagtcaccgt tcaggtatga atctgttaat tggtaacgtc
     1141 cgatataacg tccatttgta gctgtatagc taccacctga ttctttttga gcgatccatt
     1201 ctttggcttc tgcttcagat ccgcttacag ttgaaacaac tgtttcactt actactggag
     1261 tttctgctac tggagctgaa acagtttcat cttgagcggc tggtgccgca taagtagctg
     1321 gcgctggtgt tgcaacaggc gctacagggc catcgataac caactcttga tcaacataaa
     1381 tcaaatgaat gttatcaatg tggttgtttt ctgccaattt ttcaactgtt gtgttgtgag
     1441 tttcagcgat ttctgaaagt gtatcacctt ctttaacagt gtaagttgat gattcttgag
     1501 cagatacaaa tgatggagca aatactgcaa acaaggcagc tactcctgca agagttgctt
     1561 taatcttttt agttattgat ttcatattcg aaaattctcc ttctttctat agttctatca
     1621 taccctttaa atattaccgt ttcttgacac ttttatgtag aaatattaca aaattatttt
     1681 ttatttccct aaataaggtg ttttttgtca taaaagatac tatttttatg ctatttgagg
     1741 tattagaaag gttttcatcc acaattaaag ccaagcccct cattctttga tataatagag
     1801 ataagaattt ttataagggg aaactgatga aatcacttcg ttttcaatct gtctttgata
     1861 tcatcggacc agttatgatt ggcccatcta gtagccatac cgctggtgct gttcgtattg
     1921 ggaagattgt ctcttccatt tttgatgata ctccgacaga agttgaattc caacttttta
     1981 actcatttgc caagacctat cgtggtcacg gaacagacct agcccttgtt gcaggtattt
     2041 taggaatgga tacagatgat cctgaaattc caaatagtct ggaaattgcc cacaagcgtg
     2101 gtatcaagat tgtctggacc attcagaaag acagcaatgc ccctcaccct aacaccacta
     2161 aaattactgt taaaaatgcc cacaagacca tcagcgtgac tggtatttct atcggtggag
     2221 gaaatattca ggtaaccgaa ctcaatggtt ttgccgtctc tctcaatatg aatacaccga
     2281 ctatcatcat cgttcatcaa gatattccag gtatgattgc ccttgttaca gaggcgcttt
     2341 cccgctatgg tatcaatatc gcccagatga atgtcactcg tgaaaaagct ggtgaaaaag
     2401 ccattatgat tatcgaagtt gacagtcgca actgtgatga ggctatcgaa gaaattcgaa
     2461 aaatccctca tctccacaat gtcaatttct ttaaatagga ggaagcatgt tttattctat
     2521 caaagaattg gtcgagcaag cagatctgga ctttcaagga aatgtcgcag aactcatgat
     2581 tacaacagag tttgaattga ccggtcgcga acgtgaagaa gtcttccttc tcatggaacg
     2641 caatctggaa gttatgaaag cctctgtcca acttggcctc aatgaaaata aatctcgtag
     2701 tggcctgaca ggtggagatg ctgccaaatt ggatcactac atcaaaaacg aaaaaacttt
     2761 atcagattac acgattctct ctgctgcccg aaatgctatc gcagtcaatg aacacaatgc
     2821 taaaatgggc ttggtctgcg ctactcctac cgcaggaagt gccggttgtc tcccttccgt
     2881 tctcactgct gctattgaaa aattagacct cagccacgag caacaattgg atttcctctt
     2941 tgctgctggt gcctttggac tagtcatcgc aaacaatgcc tccatctcag gtgctgaggg
     3001 tgggtgtcaa gctgaagttg gttcagcctc tgctatgagt gctgccgcct tgactctggc
     3061 tgcaggtgga acaccttatc aggccagtca agctattgcc tttgtcatta aaaatatgct
     3121 aggcctcatc tgtgaccctg ttgcaggttt ggtcgaagtt ccctgtgtca aacgtaatgc
     3181 catgggagct agctttgctt tcatcgcagc agacatggcc ttggcaggta tcgaatctaa
     3241 aatccctgtg gatgaagtga tcgatgccat gtaccaagta ggagcaagca tgccaactgc
     3301 ctttcgtgaa acagctgaag gtggactcgc taccacccct actggtcgtc gcctccaaaa
     3361 agagattttc ggagaataag cttatcaaat aggagaaacc atgacctcta tcacagcgat
     3421 ttttttcgat ttggatggaa ccctcgttga tagttctatc gggattcaca atgcctttac
     3481 ctataccttt aaagagctgg gggtgcctag ccctgatgcc aaaactattc gtggttttat
     3541 gggaccacct ctcgaaagta gttttgcgac ctgcctgtcc aaagaccaaa tttctgaagc
     3601 catgcagata tatcgttctt actataaggc aaaaggcatc tatgaagctc aactctttcc
     3661 tcagattata gacttgcttg aggagttatc gagcagttat ccactctaca tcaccacgac
     3721 aaagaataca tctactgctc aagacatggc caaaaacttg gaaatccatc atttctttga
     3781 tggtatttat ggttctagcc ctgaagcacc ccataaggca gatgtcattc accaagcctt
     3841 acagacacat caactagcac cagaacaagc catcatcatc ggagatacca agtttgatat
     3901 gctgggagct cgagaaacag gtattcagaa attggccgtc acttggggat ttggagagca
     3961 agcagatcta ctaaactatc aacctgatta tatcgctcac aaacccttag aagttttggc
     4021 gtattttcaa tagcatagac tgggcgacaa ctctatttca gaagaaaatg aagtaatact
     4081 caatgaaaat caaagagcaa actaggaaac tagctgcggg ctgctcaaaa cactgtttta
     4141 aggttgtaga tggaactgac gaagtcagct caaaacactg ttttgaggtt gcagatagaa
     4201 ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
     4261 accatatacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtataagtct
     4321 ctacataata aaacgcatat taccaaggtt gttaacttga taatatgcgt ttttcttttt
     4381 tattcaatgt gggttgtttg attagcaaag gcgataatag cttgcttcaa ctcatctcca
     4441 ctgagagtgc ggaactctcc aatcttttga ttgatggatt ctaaaggcat gacattaacc
     4501 ttaccaacga aaatcttatc cataacttga ttgtcaacga gttcggttga taccaagagt
     4561 tcttcgtaga gtttttcacc tgggcggatt ccaacttcaa cgattggaat ttcactttca
     4621 gtgtggccac ttagaagcac catcttcttg gccaagtcat aaatcttgac tggtttgccc
     4681 atatcaagga taaagacttc cccatctttg gcataagcac cagcatggat aaccagacgg
     4741 ctagcttctg gaatggtcat aaagtaacgg gtcatgcgga agtctgtcac cgttacaggc
     4801 ccaccttcag caatctgacg ttcaaagact ggaatgacac taccacggct accaagaaca
     4861 ttcccaaaac gaactgcaca gtaggttgat tggctacgtt ggttaaagcc agtgacaatc
     4921 aactccgcca cgcgcttggt tgctcccata acatttggtg gattaactgc cttatctgtc
     4981 gaaatcataa ccatcttagg caccttggct tcatcaacgg ctctagcaac attgtaagtt
     5041 ccacggatat tgtttttgaa ggcttctttt ggattgcgct ccatcatagg aacatgcttg
     5101 tgggctgccg catgataaac aatagcaggt ttgtactgct caaagacttg caacaaacga
     5161 tcatagtctt gaatgtccgc aatcacaggt acataatcaa tcccttggaa cttacgaatc
     5221 aattcatgat aaacaaggta gattgagttt tccccatgac cgagcaagac aatgcgttca
     5281 ggattgaagc gactaacttg acgacagatt tcagaaccga ttgaacctcc agctcctgtg
     5341 actaagatgg tcttacctgt cagttctgca cccagacgcg attcgtcaag acggatttcc
     5401 tgacgaccca aaaggtccgt aatatcaatt ttttggaagc cagtacctgc ttggtgaagg
     5461 ccctgaacaa cagtttcaac cttaggcatc ttgtaacatt tgacacccag cttattacac
     5521 atctgcaaga tacgctcata ttctgacgga tccagcgacg gaatcgcaac gatgacacgc
     5581 tcgatttgat ggcgtttggc taattcaggc agattgtcat aagagcccaa aacaggaata
     5641 ccaccaagtt tttgaccctt tttcttagaa tccttatcca aaataccgac cagttctaat
     5701 tcactggttg gatgttggta actatccata aagagagccc caccatcacc ggcaccaatc
     5761 aagaaggtcc gacggtgttc tccatcacca ctaccttttt tgcgtctgga gtagattaac
     5821 tgccaagtaa tccgtggcaa taaaatcaag aaggtactca acaagataaa gagaatgatg
     5881 aaacggatgg agaagagtgg caagaaggca taacagatac tatatgacaa gacactgcta
     5941 gcagtcacac caaaaaagat tttcatgaaa tccgtaatct tgctgtaacg actaatgctc
     6001 gcgttcaacc cccaaaaacc aatcatcaat tgatagaaca agaaggccaa actcgtatag
     6061 ataatgtagt caacaggtgc tggattaatc agcccataaa ataaaatata agatacaatg
     6121 atggaaacca ccatactgaa aatatcaaat attccccaga aaacctgctt ttgttgttta
     6181 tttaaaattt ccaccagatc aatcacataa tctgttagtt ttttattcat agaaatctac
     6241 tcctccttaa aagagtttta ctatattgtt attgtagcat attttgattt atttggctta
     6301 ttttatccaa ataaattatt gttcccttgt actccttctt ccatttaaat cctctaatta
     6361 aacctacaat cgtcccaaag ccataagcaa agtgaatgga aaataaaaga aagggcatca
     6421 caattagaaa tccattttta tgttttaata aagtcagcaa agtgagtagt gacaaaagta
     6481 gaaaataggc acctaatagt aaagttatga atacgaatgt gatcggtaac aatgctagac
     6541 taaacacaag actcaaaaca aataaacaag gaacatagtg aaataatgat aaacacttaa
     6601 actgaacatg acttgtcaag ccaatccaca aaccatttga atacttttga tgcagcattt
     6661 tcttgaatgt tggtcgaata tactgataag atagaatact tgggctatag cggattttat
     6721 aaccatgttc tcgaattcta taatgaatat cattatcttc agttcggcca agttgctcat
     6781 ttactaaacc aaccttctgg aaaacctctc gtttatacat cccatgaaaa atagaagaaa
     6841 catatctatc ctcggaacta tttcgataat tggcaatgct actgccaaac atattttcct
     6901 caacaagatg caaggtctct gcccattttc cttttccttc gacaatcgtc ggtctaggcc
     6961 ccccacagac aaattcacct tgttgaataa tagccacatt gttcattaca aaagtctcag
     7021 taacttttga atgagcatca atttttaaaa taaggtcccc tacagaatgt ttaactccca
     7081 ggttaaaacc actagcttga tttttcttag gattgttata caatctaatt gagttaaact
     7141 ctgtatcttc ctttataaat tgctgaatga tagctgtggt cccatctgtg gacatagcat
     7201 ttataaatag aatttcaata ttctctttag gataagtttg attttttaag tcttcaatta
     7261 gaccaggcag atatttttct tcattgtatg cactgattac aatggatacg agaagacttt
     7321 caacattttg agtcactact actctcctga atttttcctt ccaccattct atcataattt
     7381 tcaagacttt cccattttta tcttgaaagc cagaaacctt acttgacatt atagtgaaaa
     7441 agccttaaaa taagctgtta ttaaaatcag ctagaagagg tattatatga aaaagcaatc
     7501 actctttttt gttccaggta ttatcctgat tggtgtttcc ttgcgaactc ctttcactgt
     7561 tttacccatt attttgggaa atatttcgca aggtctggag gtagaagtta gttcgcttgg
     7621 tgtcttgacc agcctgcctc tccttatgtt taccctcttt tcaccatttt ctacccaact
     7681 ggctcagaaa atcggcttgg agcatctctt cacctacagc ctcttcttct tgaccatcgg
     7741 ctcactcatt cgactaatca atctgcccct gctctatcta ggaaccttga tggttggggc
     7801 aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc caagccaatc aaccaaagaa
     7861 aattggtttt ctgaccacct tatatgtaac gtctatgggg attgcaacgg ctctggcttc
     7921 ctatctagct gtgcccatta cacaagccag ttcttggaaa ggacttatcc tcctcctcac
     7981 gttactctgt ctagcaactt ttttggtctg gctcccaaat caccgctata atcatagact
     8041 agctccacaa accaaacaaa aaagtcaaat aaaggtcatg cgtaataaac aggtttgggc
     8101 aattattatc ttttcaggtt ttcaatcctt gatcttttac accgccatga cctggttacc
     8161 taccatgtct atccatgcag gtctatccag tcacgaagct ggcttgctga cttctatctt
     8221 atctctgatt agcattcctt tttcaatgac catcccaagc ctgacaacca gtttatctac
     8281 tcgcaaccgt cagctcatgc tcactctggt ttcactagct ggtgtggtcg gcatttccat
     8341 gctctttttc ccaatcaata atttcattta ctggcttgcc atccatctcc tcatcggaac
     8401 cgcaaccagc gccctcttcc cttatctcat ggtcaacttt tcactcaaga caagcgcccc
     8461 tgaaaagaca gcccaattgt ccggcctatc tcaaacagga ggctatatcc tagcagcctt
     8521 tgggccaacc ctctttggtt acagttttga cctattccac tcttgggtac cagctgtagc
     8581 tgccctcttg ctcatcgata tcctgatgac tgtggccctc tttacagtgg acagagcgga
     8641 taagatcctt taaacttcta gttttagcta gaagtttttt atatataaaa attttacaca
     8701 tttctcttga cagggctttc ttttagatgt acaatgtatg tgtagaaaaa ttatatataa
     8761 aaatcttaca cattagaaaa ggaggtttcc catgtacttt ccaacatcct ctgccttgat
     8821 tgaatttctc atcttggctg tactggagca gggtgattct tatggttatg agattagcca
     8881 aaccattaag ctgatcgcta atatcaaaga atccacactc tatcccattc tcaaaaaatt
     8941 ggaaggcaat agctttctga caacctattc tagagagttc caaggtcgca tgcgcaaata
     9001 ctactccttg acaaacggtg gtatagagca gctcttgacc ctaaaagatg aatggacact
     9061 ctatacagac accatcaatg gcatcataga agggagtatc cgccatgaca agaactgaat
     9121 acctgactca gctagaactc tatctcaaga aactacctga agctgaccgt atcgaagcca
     9181 tggactattt cagagagctc tttgacgatg ctggagtcga aggagaagaa gaactcatcg
     9241 ctagtttggg aactcccaaa gaagtggccc acgaagttct atccaatctt ctcgataaaa
     9301 aaatcaatga agcacccgct caaaaaaata accgacaaat tttacatatc gccttgttag
     9361 ccctccttgc agcacctatc ggcattcctc tgggaatcgc catcctcgtg accctgttcg
     9421 caatccttgt agccgctttg actgtcattc tggctttctt tgcagtttcc atactgggta
     9481 tcatcggcgg attcctattt ttagttgaaa gtttcactgt cctcgcccaa gccaaatcag
     9541 cctttatctt gatttttggt tctggtttac tggctatcgg tgcttcttcg ctagttttac
     9601 ttggcatttc ctatgtagct cgcttcttcg gtctactcat tgttcgtctg gtacaatttg
     9661 ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat ggacaaaagg atttctcatc
     9721 tttggtgtgg tgactaccgt tatcggcttt attctgcttt ttgtaggtat ccaatctgac
     9781 gggattaaga gcctactttc catgtccaaa gaacctgtct atgatagccg tacggaaaag
     9841 ctaacctttg gcaaggaagt cgaaaaccta gaaattactc tccaccaaca cacgctcacc
     9901 atcacagact ctttcgatga tcaaatccac atttcttacc atccatctct ttctgctcac
     9961 catgatctta tcaccaatca gaacgataga actctgagtc tcactgataa gaaactgtct
    10021 gaaactccgt ttctctcttc tggaattggt gggattcttc atatcgcaag tagctactct
    10081 agtcgttttg aagaagttat tctccgacta ccaaaaggga gaactctaaa agggatcaac
    10141 atctcagcca atcgcggaca aaccaccatc ataaatgcta gccttgaaaa tgcgaccctc
    10201 aatacaaaca gctatatcct ccgaattgaa ggaagtcgta tcaaaaacag taaactcaca
    10261 acgcccaata tcgttaatat ctttgataca gttcttacag atagtcagct agagtcaaca
    10321 gagaatcact tccacgctga aaatatccaa gtccatggta aggttgaact gactgccaaa
    10381 gattatctca gaatcatcct agaccagaaa gaaagccaac gaattaactg ggacatctca
    10441 agcaactatg gttctatctt ccaattcaca agagaaaagc ctgaatcaag aggtacggaa
    10501 ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg atcaactcat tgcgagatct
    10561 gatgataata ttgatctaat atccacacca agcagacgtt gacaaaaacg cttacatctg
    10621 ctactatgga agcatattta tctaactaaa aaggaggttc taccatgaaa caagaatggt
    10681 ttgaaagtaa tgattttgta aaaacaacaa gcaagaacaa gcctgaagag caagctcaag
    10741 aggttgcaga caaggctgaa gaaacgatag ccgatctcga tacaccaatt ggaaaaaata
    10801 ctcaagtaga ggaggaagtc tctcaagctg aagtcgaact ggaaagccag caagaagaga
    10861 aaattgaaac tcctgaaggc ggtgaagcga gaacagaaat agaagaaaag aaggcatcta
    10921 attctactga agaagagcca gacctttcta aagaaacaga aaaagtcact atagctgaag
    10981 agagccaaga agctcttcct cagcaaaaag caaccacgaa agagccactt cttatcagta
    11041 aatctttaga aagtccttat atccccgacc aagctccaaa atctagggat aaatggaaag
    11101 agcaagtgct tgatttttgg tcttggctag tggaagcgat caaatctcct acaagtaagt
    11161 tggaaacaag tatcacacac agttacacag cctttctctt gctcattctg ttttctgcat
    11221 cttccttttt ctttagtatc tatcacatca aacatgctta ctatggacat atagcaagca
    11281 ttaacagtcg cttccctgag cagctagctc ctttaactct tttttctatc atctctatcc
    11341 tagtagcgac aacactcttc ttcttttcat tcctcttggg tagtttcgtt gtgagacgat
    11401 ttatccacca ggaaaaggac tggacgctag acaaggttct ccaacaatat agtcaactct
    11461 tggcaattcc aatcttcctc actgctattg ctagtttctt tgctttcttt gatagcctac
    11521 gatttacagc cctcttgtgt gtgattagca ttggaatcat tctgcttgcc agtctccata
    11581 tcattacaag accaagtcaa gcaagtgaaa ccgactcctt ctatcaatta ttcttgtctg
    11641 tccttgtgaa cggagttatt atcctcctct tctttgtagc tgaagtggca ctgattggag
    11701 attatcttcg tatcttggcc tttctttaaa cttcatactc ttcgaaaatc aaattcaaac
    11761 cacgtcagct tcgccttacc gtactcaagt acagcctgcg gctagcttcc tagtttgctc
    11821 tttgattttc attgagtagc aatcctgtca tccttggcag gattttttct atgtcaaaaa
    11881 agcaagtcga ttgacctgct tctgctttac ttttcttgtg ctaatgtttt aaaatctttg
    11941 tctaatatgg gttgcatttc tatttgattg gcgtctagtt ggtggtaaaa agatgggggt
    12001 aatacctcta agaattttgc atagtccaag cgggcaatgg cttcatagtc ttctggttta
    12061 gagccgtctc ctgtgatttg aaccaagtca atctcccact ggacttcctt atccaccaat
    12121 tgctcaatat aggctgcagg aacaaacggt tctctcggta aaactgtctt gactccttga
    12181 gctagatggt aaacgccatg cacttttcct tctccatcct gatagaagga aactctcgca
    12241 aagtaggcat ctgtctcttg aaccgcacgc acacgcgctt caaactgagc caagccatct
    12301 gcctctaaaa agcttttcaa gttctcacga ctaaagaaaa taggattaaa aattccttgg
    12361 caaatcgtaa aacgagctac agggtctgct agataggctt gaatacttgc ttggaaataa
    12421 gcttcaaaat caaaaccatc tagcccttca atatcagtcc ctgtataagc aagcaaagca
    12481 tctaaaaatg acttgataat ctgccagtct gtctttgtta gtaggtcagg aagatcgaca
    12541 cggtaagcct tttgaccatc agcataactg accttaaaaa gaaattgtga ttgccccact
    12601 atcgcacact caatatactc gagacgatta agaggctgac ggagatagac tacatcatag
    12661 ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact tggcattcaa aatctcttgt
    12721 tctcctaaaa tgctctgttt atttggaata aaaaatgttt tcgccataac tggactcctt
    12781 tgaaatcgtt tacatacagt ataccttatt tttctgaaag atacaaaaac aaactttata
    12841 ttcttcgaaa atctcttcaa accgcgtcag cttcgccttg ccgtagatat atgtaactga
    12901 cttcgtcagt cttatctaca acctcaaagc agtgctttga gcatcctgca gctagcttcc
    12961 tagtttgctc tttgattttc attgagtatt agatttttga gtacaaagca tagaaaaaca
    13021 gcccctgagg actgtttgat cttatacagt agctgcctga tccaagcttt caccgatagc
    13081 ggctaggcgc tcaatcactt cagcttgtgt caattcattt tttgaaacat agcggttacg
    13141 tgggtgaaca cggcactcgt gtgagcatcc acgaaggtac ttgtcttcat tttcttctga
    13201 tgtcaagata cgacggttac agaagggatt tccacagttg acataacgtt cacatggtgt
    13261 tccatcaaac cagtctttcc ctacgatggt tgggttgaca tggttgacat caacggcaat
    13321 acgctcgtca aagacgtaca ttttcccatc ccaaagctca ccttgaactt ctgggtcttt
    13381 accgtaagtt gcgattcctc cgtgcaattg gccgacatct ttgtagcctt cacggaccat
    13441 ccagcctgag aatttctcac agcgaacgcc acctgtacag taaaccacga cacgcttgtc
    13501 catgaatttt tccttgttat cacggaccca ttgtggtaac tcacggaagt tgcgaatatc
    13561 tgggcgaata gccccacgga aatgtcctag gtcgtactca taatcgttac gtgtgtcaag
    13621 gacaacggta tctttatcaa gaagcgcttc tttgaactct tttggagaca agtaagcacc
    13681 tgttgtttca agtgggttga tgtcattgtc aaagtcgttg tcttccaaac caaggtggac
    13741 aatttctttc ttgtagcgaa caaacatctt cttgaaggct tgttcatttt cttcgtcaat
    13801 cttgaaccag agttcttcca ttcctggaag gctgtgaacg tagtccatgt atttttgagt
    13861 tgtttcatag tcacctgaaa ctgttccgtt aattccctcg tcagcgacta ggatacggcc
    13921 tttaaggccg attgatttac agaaagccaa gtggtctgca gcaaattgct ctgcattttc
    13981 aattggagta taaaggtagt aaagtaagac acgaatatct tttgccataa gatttgttct
    14041 cttttctatt cttaaatttt ctaaattttt tatttatcta cactagtata cctgcttgag
    14101 aaaacgaatg caatttattt gactggaaat tgtagagggt tctcgaaatt tcctgaatgg
    14161 ttaaaataag gacaagagaa aacatggata tctatatcct tgtgccaaaa aaaccactgc
    14221 cctccccaga ccaacctgag gaaagcagtg attcttattt taggagttag gaatgaatac
    14281 acgaaatcaa tttagctgat tattttttgt ttttcaagaa ttcatcgtat tgtttttgca
    14341 tttcgttcaa tactttttcg taggcacctt cagatttcaa tttttccatc aattctggaa
    14401 tcgctttatc tgggtctaca gtaccagtgt tgatagctgt atcaaattgt tgcattgtgt
    14461 tagcaatagc tgagatttca gatttcacat tgtcagtatt gaagataaat ccaagcgctg
    14521 gagattcttt agcttctgcc aattctttct tagaattttc gatttgttgg tctgtaacgt
    14581 tttcgttgat gtaaaggatc cagttgttac cagtgttcca tccacccatg tgagtgtttc
    14641 ctttgtagcc atcaagaacg cgaacacggt tttctttacc ttcaattttt tcccagttct
    14701 tgccttctgg accgtaaaca agaccgttca agagttctgg attcgtattc aagaggttca
    14761 agatttccat tgatttttct ttgttcttag agttgtttga gatgacaaag ttagcaactt
    14821 gtgttgtttg gtttttcttg atgaagttag taattggttt gatttggata tctttgttgg
    14881 caacacgtga aagcaagctg ttaccgtagt cagctggtcc tactgtttct tcacgaacga
    14941 accaagtatc ttgttgaagg tcaaaggaag tatcgcttgt tgcgacgtct tttggaatgt
    15001 agccagcttc atagaatttg tgaagagtct tcaagtgttc tttgaaacga ggcacttcgt
    15061 aacggtttac aactttagta gtatcgcctt caaggtcgat aacgaatgga agaccgtttg
    15121 ctactgggta gtcaaagtta tcagatggga tgaaaacttt accaatagca aatggtacta
    15181 cgtctggagc tttttctttg atttgtttca agactggctc aagagtttcg taagaagtaa
    15241 cacctgaaat atcgatacca tatttagcaa ggagagttcc gttgaaggca aagttttgag
    15301 atgatgcaac gttggctgca actggaacag cgtaaatctt accatttaca gtattaccct
    15361 tgatgtaagc tgggtcaagt gctttgtaaa ggtctttacc ttcttttttg tacaattctg
    15421 tcaagtcagc gtaagcacct ttttgagcat ttacaatata gttatctgca aaggcaatat
    15481 catagttttc accagatgat gtgataactg acattttctt accatagtca ccccagccaa
    15541 ggtattggat atccaatttg gcaccaactt tttcttcgat gattttgttg gcatttgcta
    15601 acaattcatc caagttgtct ggtttgtcac cgatttggta cattttgata acaggtttgt
    15661 cacctgaatc agcagctttt ttgctgttac ctgtcaaatt tccacaagca gcaagacctg
    15721 cagccagagc gactacacta gcagatgcaa aagcatattt tttccagttt ttcatgataa
    15781 aaactccttt ttttattttt aaacttataa acaatgtaat
//
LOCUS       DAVMHG010000039        15396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMHG010000039 DAVMHG010000000
VERSION     DAVMHG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.039"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..1534
                     /locus_tag="VYR08_000975"
     CDS             104..1534
                     /locus_tag="VYR08_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV3466519.1"
                     /translation="MCVTNFTESEQWDTVIEGNDYLLEKYMSGKSLEALELEQEESIR
                     FQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRL
                     AYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDS
                     TTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPN
                     PFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDC
                     KICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLLRAY
                     NDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVT
                     TGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1653..1820
                     /locus_tag="VYR08_000976"
     CDS             1653..1820
                     /locus_tag="VYR08_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV3466520.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1880..2233)
                     /locus_tag="VYR08_000977"
     CDS             complement(1880..2233)
                     /locus_tag="VYR08_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466521.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            2738..3160
                     /locus_tag="VYR08_000978"
     CDS             2738..3160
                     /locus_tag="VYR08_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3466522.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3157..3387
                     /locus_tag="VYR08_000979"
     CDS             3157..3387
                     /locus_tag="VYR08_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466523.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            3848..4051
                     /locus_tag="VYR08_000980"
     CDS             3848..4051
                     /locus_tag="VYR08_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV3466524.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4133..5350
                     /locus_tag="VYR08_000981"
     CDS             4133..5350
                     /locus_tag="VYR08_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3466525.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYND
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMSEMK
                     RLNKEKQQERLVA"
     gene            5587..5748
                     /locus_tag="VYR08_000982"
     CDS             5587..5748
                     /locus_tag="VYR08_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3466526.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            5735..5941
                     /locus_tag="VYR08_000983"
     CDS             5735..5941
                     /locus_tag="VYR08_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466527.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            5987..6346
                     /locus_tag="VYR08_000984"
     CDS             5987..6346
                     /locus_tag="VYR08_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466528.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMASG"
     gene            6475..6900
                     /locus_tag="VYR08_000985"
     CDS             6475..6900
                     /locus_tag="VYR08_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466529.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            7308..8102
                     /locus_tag="VYR08_000986"
     CDS             7308..8102
                     /locus_tag="VYR08_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466530.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            8106..8303
                     /locus_tag="VYR08_000987"
     CDS             8106..8303
                     /locus_tag="VYR08_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466531.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDSISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            8293..8844
                     /locus_tag="VYR08_000988"
     CDS             8293..8844
                     /locus_tag="VYR08_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466532.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFHSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            8903..9163
                     /locus_tag="VYR08_000989"
     CDS             8903..9163
                     /locus_tag="VYR08_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466533.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(9927..10729)
                     /locus_tag="VYR08_000990"
     CDS             complement(join(9927..10279,10279..10729))
                     /locus_tag="VYR08_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV3466534.1"
                     /translation="MNYEASKQLTDAQFKRLVSVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSQGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            10854..11312
                     /locus_tag="VYR08_000991"
     CDS             10854..11312
                     /locus_tag="VYR08_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV3466535.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            11325..11933
                     /locus_tag="VYR08_000992"
     CDS             11325..11933
                     /locus_tag="VYR08_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466536.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            12004..12336
                     /locus_tag="VYR08_000993"
     CDS             12004..12336
                     /locus_tag="VYR08_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466537.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            12347..13762
                     /gene="ply"
                     /locus_tag="VYR08_000994"
     CDS             12347..13762
                     /gene="ply"
                     /locus_tag="VYR08_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV3466538.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            14645..15361
                     /locus_tag="VYR08_000995"
     CDS             14645..15361
                     /locus_tag="VYR08_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3466539.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
BASE COUNT         5104 a         2480 c         3148 g         4664 t
ORIGIN      
        1 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
       61 tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt
      121 taccgaatct gaacaatggg atacggtaat agagggaaac gattaccttt tagagaaata
      181 tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca
      241 gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa
      301 ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact
      361 ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg
      421 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
      481 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
      541 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
      601 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
      661 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
      721 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
      781 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
      841 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
      901 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
      961 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     1021 aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt
     1081 gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc
     1141 tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa
     1201 aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga
     1261 atatctttta cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca
     1321 attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata
     1381 tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg
     1441 gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga
     1501 taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat
     1561 ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg
     1621 aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac
     1681 gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca
     1741 gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg
     1801 caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt
     1861 tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta
     1921 aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc tctatctgta
     1981 atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat
     2041 gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa
     2101 tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac
     2161 ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt
     2221 cttttttacg catagtagac cacctctata cattttattg ttcctactga attaaaaaca
     2281 ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa
     2341 cgccaataaa aaaaaccgtt atatggtagt gctatttacg ctgttaaaat attgtatatt
     2401 acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca tatacaataa
     2461 atttccttac attgggttct tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc
     2521 caccaatgtg gttttataaa tcatatagat agaataacag aagcatgtaa acagagaaat
     2581 aaatctgttt atatgctttt ttggctattc agaacttttt tacaaagttt atttatcagt
     2641 aatgcaacaa atcccccttt cacattggga ctaagagtga aaggagataa acgagcaagg
     2701 ctcacttcct ttcctagaca gaaagggggt gagaaacatg aaaccatctt cttttcagac
     2761 cacaatagaa aatcagtttg actatatctg taaacgtgct atggaagacg agcgaaagaa
     2821 ttatatgctt tatctttcaa ggattgcaaa gcgtgaggtg tccttttcgg atgttggcga
     2881 ttatcttgtt agccagtttg cgacaacaga taactattca actgactttc agatttttac
     2941 actcaatggg ttatcagtag gcgttgaaaa tgatttgttg agtgaagcat tacgtgagtt
     3001 gccagacaag aaacgtgaaa ttctactgct gttttacttt atggacatga gcgattcaga
     3061 aattgcagac ctgttgaaat tgaaccgttc tactgtctat cggcatagaa ccagtggact
     3121 agccttaatt aaaaagttta tggaggaatt tgaagaatga aaacacaata tcctatgatt
     3181 ccctttcctc tcattgtaaa ggcaacagat ggcgataccg aagcgattaa ccagattcta
     3241 catcattaca gagggtacat aacgaagcgt tccctacgac ttatgaaaga tgaatatggc
     3301 aatcaaagta tggtcgttga tgaagtctta cgtggaagaa tggaaaccag actgattaca
     3361 aagattttgt catttgaaat taagtaatat cctctctcct ttcgtggaag cgtgctaaac
     3421 cattccacgc ttcccgaaca gggaggtttg ttattccacc aaagcatatt gagctttcaa
     3481 tgtgttttga taggctaacg agccattgtt ctttgaaaac tgaataaaag taatcgaata
     3541 cgtttcgata agaaaagagc caacggaact aaccgccatg acctatctta taaagatagc
     3601 gagcgattca tgttagtgat ccgagaagca atctttagca ggattgcctg caacgacatt
     3661 cttatcgtga taatgatact cccatacagt caatagtccg agcgtgataa aaccgtcgca
     3721 ggcaatgagt atggctacat gagaaccatg caggggtgga actcccgtga gctttgctaa
     3781 agctgttcga ttgctggtaa aacaactttt atgaaatcca aataagtgat ttggaaagga
     3841 ggattttatg aagcagactg acattcctat ttgggaacgt tataccctaa ccattgaaga
     3901 agcgtcaaaa tattttcgta ttggcgaaaa caagctacga cgcttggcag aggaaaataa
     3961 aaatgcaaat tggctgatta tgaatggcaa tcgtattcag attaaacgaa aacaatttga
     4021 aaaaattata gatacattgg acgcaatcta gcgtcgccaa agggtcttgt atatgataaa
     4081 atagtattaa gtcgtatcaa ggctctttcc ataaaggaaa ggagcaaatg ccatgtcaga
     4141 aaaaagacgt gacaataaag gtcgaatctt aaagactgga gagagccaac gaaaagacgg
     4201 aagatactta tacaaatata tagattcatt tggagaaccg caatttgttt actcgtggaa
     4261 acttgtggct acagaccgag taccagcagg aaagcgtgat tgtatctcac ttagagagaa
     4321 aatcgcagag ttacagaaag acattcatga tggtattgat gttgtaggaa agaaaatgac
     4381 actctgccag ctttacgcaa aacagaacgc tcaaagacca aaggttagaa aaaacactga
     4441 aactggacgc aaatatctta tggatatttt gaagaaagac aagttaggtg taagaagtat
     4501 tgacagtatt aagccatcag acgctaaaga atgggctatt agaatgagtg aaaatggtta
     4561 tgcttatcaa accatcaata actacaaacg ttctttaaag gcttcattct atattgctat
     4621 acaagatgat tgtgttcgga agaatccatt tgactttcaa ctgaaagcag ttcttgatga
     4681 tgatactgtc cctaagaccg tactaacaga agaacaggaa gaaaaactgt tagcctttgc
     4741 aaaagctgat aaaacctaca gcaaaaatta tgatgaaatt ctgatactct taaaaacagg
     4801 tcttcgtatt tcagagtttg gtggtttgac acttccagat ttagattttg agaatcgtct
     4861 tgtcaatata gaccatcagc tattgagaga tactgaaatt gggtactaca ttgaaacacc
     4921 aaagaccaaa agtggcgaac gtcaagttcc tatggttgaa gaagcctatc aagcatttaa
     4981 gcgagtgtta gcgaatcgaa agaatgataa gcgtgttgag attgatggat atagtgattt
     5041 cctctttctt aatagaaaga actatccaaa agtggcgagt gactataatg gtatgatgaa
     5101 aggtcttgtt aagaaataca acaagtataa tgatgataag ttaccacaca tcactccaca
     5161 tagtttgcga catacattct gtaccaacta tgcaaatgca ggaatgaacc caaaagcatt
     5221 acaatacatt atggggcatg caaatatagc catgacgctg aactattacg cacacgcaac
     5281 atttgattcc gcaatgtcag agatgaaacg cttgaataaa gagaagcaac aggagcgtct
     5341 tgttgcttag tagtacaaat gaatttacta cttatttacc acttctgaca gctaagacat
     5401 gaggaaatat gcaaagaaac gtgaagtatc ttcctacagt aaaaatactc gaaagcacat
     5461 agaataaggc tttacgagca tttaagaaaa tataaaaaga taattagaaa tttatacttt
     5521 gtttatatat aaaaattatg acaagagtgt gctattcttt ttatgagagg tgtatgaata
     5581 tgataaatgt atgtgataaa tgtatgtgat gttggaaaaa gaataaaaga acttagaata
     5641 tcttcaaatc ttactcaaga taagattgct gagtatttgt ctttgaatca aagcatgatt
     5701 gccaaaatgg aaaaaggtga aaggaatatc acgaatggat ttaagtaata aagcttcaaa
     5761 tcttagaaaa aagttgggag ctgatggtga atcgccgata gatattttta aattggtaca
     5821 aaagatagaa aatttgacgc tggtatttta tggactcgga aagaatatta gcggagtctg
     5881 ttataaagga actcagttca gtctcattgc agtcaattca gacatgccat taggaaggta
     5941 aagattttct ttagcacatg aactgtatca tctttattat gatgaggtga agaagagttc
     6001 agtcagtctt atcttgattg gtgaaggaga tgaaactgaa agaaaagcgg atcagtttgc
     6061 ttcttatttt ttaattttcc catcttcact gtataggatg gttgaggaaa tcagagaaaa
     6121 tgccaataga actcatcttg aagtagaaga tattataaaa ttgggtcagt tttatggtat
     6181 cagtcataaa gctatgttat atagattgag gaatgatgga taccttgatg cagaagaaat
     6241 taaaaatatg gatattagtg ttatagagac agcttcaaga ttaggctatg atacaagttt
     6301 atatcgtcct ttgtcagaaa gtaaaaaaga aatggcatca ggataatata ttaattcaac
     6361 tgaacaactt ttagaaaata acagaatttc gcaagggaag tatgaggaac tgttactaga
     6421 tgctttcaga tatgatattg tatatgggct agatgaagag gggggagttg tcgtttgact
     6481 agtcgtgtat ttattgatgc agattgtatt tcagtatttt tatgggttgg cactgaacat
     6541 cttttagaaa agctctattt gggtaaaatt gttattccac aagaggtgta tgatgaaatc
     6601 aatataccta caattcccca tttaaaatct aggatagacc agttggtaac taagggttca
     6661 gctgagattg tgagcataga cattggaact gaagaatacg cattatatag agatttaaca
     6721 agaaatcatg atagtaacaa gattattggt aagggagaag cgacttctat tttcttagcg
     6781 aaaaagcata atgggatatt aggaagtaat aacctaagag atgttaaacc atatgtagaa
     6841 gaattttctt tagaacatat gacaacagga gatatactga ttgaagcgtt taaagcgtaa
     6901 tttattactg aataagaggg caatcatatc tggaataata tgcttaaaaa gagaaggaaa
     6961 attggtgcaa attcattttc agactatctt cgtggaagta ttcatcaaaa tagacaaaaa
     7021 taaatttgga taaatcgaac tcactattca ggaggcatat gagcaattcg aaaaagaaaa
     7081 gtgtcaaatt gagcctatag gagtagaagt gaaatagtaa gtcctgcata gtggatgaga
     7141 gaaaagttct ccttgaagtt ttcctgaact atcagtcgca tgtcaaacga tatgtagggt
     7201 aatgtgagag gggatagcga gtagtttttg gttattttat caaaaaactt atattttatt
     7261 ataccgaatg ataaaatata ataaaaatga tagaataagg aaaaaacatg aatgtcaaaa
     7321 agataatgtc aatttttcaa tccttttatg ttgatgtcag tattgaggaa ctgactttga
     7381 ctttaccaat cagttttgta aaaaggtttg agtatactca aatgactttt cataaggaat
     7441 catttttatt gattaaagaa aagagaaggg ggagtttgag ttcatttgtt actcaggctc
     7501 gcactatggg tgaaaaagcc aatatggatg ttgttttggt gttttcgaag ttatcagaca
     7561 gtgaaaaaaa gcaattactt caagctagag ttccgtttgt agactttaag ggaaacctct
     7621 tcttccctcc attgggacta gtactcaatg cgaatgatac tgaaatccct aaggaattaa
     7681 cacctagcga acaattaacg tggattgcct ttttattgac aaaaggtcaa aaagtagtaa
     7741 atgttgattt gctttcacaa gtcactggac ttccaaactc aacaatttat aggtgtttga
     7801 ggacttttaa agctttatat tggttaaaca agcaaaataa gctttacaca tatacggtgt
     7861 caaagaaaga attattctta aaatccgtgt catgtttatt taatcccatc aaaaaacgga
     7921 ttttattgcc agatggcgat ataaagcaga taaaatctgt ttctaacctt ctatatggtg
     7981 gtgcttatgc tttgtcgcat tcaacttttt tagctgaaac ggatgaaaat actagctatg
     8041 tcatatggca gagaaaattc aatcagttat ccttgccact ttctcagcat gttttaaaat
     8101 gaaagatgct agagatatgg aaatatcgtc cttttgtatc tgagttttgg aatgatttta
     8161 aaaataatca tgataaacaa tttgtagatt cgatttctct ttatttgacc ttaaaagatg
     8221 atgatgaccc acgtatagag gaagagagtg aagcactaga aaatatgata ttacagtatc
     8281 tgggagaaga tgatgccagc taatacgaaa gttatttttc aagaaatgtt tgcggatttt
     8341 cagaactatt atgttctgat tgggggaact gctacctcta tcgtattgga ttcgcaagga
     8401 tttaaaagtc gcacaacaaa agattatgat atggtcatca ttgatgaagt aaaaaataag
     8461 gaattttata ctaccttgaa tcacttttta gaattgggag agtatcaagg aagtcagaaa
     8521 gatgagaaag cgcagctttt tcgatttaca acaactaatc ctgagtttca ttctatgatt
     8581 gaactattta gtattttacc agaatatcca ttaaagaagg acggtcgaga aattccctta
     8641 tattttgacc aagatgctag tttatcagcc ttattattgg atgaagatta ttataatata
     8701 ttggtgcatg aaaaagaaac cattcagggg tattcggtat tgagtaattg tggtctatac
     8761 tcttcgaaaa tctcttcaaa ccacgtcagc ttccatctac aacctcaaaa cagtgttttg
     8821 agcagcctgc agctagcttc ctagtttgct ctttgatttt cattgagtat taattatttt
     8881 taaggctaaa gcttggctgg atatgagaga gcgctctgct acaggtgctc aagatttaag
     8941 taagtccatt aaaaagcatt tgaatgacct tacccgtttg acagcttcct tgctaggaga
     9001 tgaaaagtta tcggctataa catcaagtag tgcggtaaaa gcagacatgc accgctttgt
     9061 gatagaatta gagcctgtga agtcaactat tcttcaaaat gatgacattt cattggatca
     9121 aaatgaaatt tttgaaattc tgaaaaattt tctcgatggt taaaataatt gtagcgagat
     9181 ggctatattg aattcgtcta tatctggaaa ctagaaaaaa cttcaatttc aggagaaaat
     9241 gaagtcaatc ttcccacaat caaacgtata gtatcaaggt ttttcaagac ctgatattat
     9301 gcgttttttg cttttcaaaa ctttttgccc agtctttgtt tttatcctct agtcacttga
     9361 tttgtttcag gtggtttttt agtatagtaa aatgaaacga gaacaggaca aattgatcag
     9421 gacagtcaaa tcgatttcta acaatgtttt agaagcagag gtgtactatt ctagtttcaa
     9481 tctactatag ttaaatctgc ggtcaagtct actggtgaat ctatgattgt aatactcttc
     9541 caaaatctca tcaaccacgt cagtcttgcc ttgcagtctg tatcttactg accaagctag
     9601 tgatggattt agaataggtg atttggagcg tcctattggc taggaaatgc tgctcatagt
     9661 cctttgctga ggctagggtg tttcaacatt caacactcaa ctggttgatc tagttgatag
     9721 gaagggagtt actataaaat actcaggctt ccatcatatt ttttgaaacg attgtgtaat
     9781 caaaatgtac caatattgta gtattggtac agaagatgtt gtgtatggat aaatatatca
     9841 taactgctgt ctctctatgt aaaaagaagg atgtatcaaa aagatttcat atgtctgtgc
     9901 atatatataa tagacttcct gcaaaactag aatcctagtt catgattgat aataccagca
     9961 atcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt tgaaaacatt
    10021 ttaaacgttt ttactttggc aaagatgttc tcaaccttgc ttctctcctt ggatagcgca
    10081 tggttacagg ctttatcttc agctgttagc ggcttgagtt tgctggattt acgtggagtt
    10141 tgtgcttgag gatatatctt catgagccct tgataaccac tgtcagccaa gattttacca
    10201 gcttgtccga tatttctgcg actcattttg aacaacttca tatcatgaca atagttcaca
    10261 gtgatatcca aagaaacaat ctcccttgac ttgtgacaat cgcttgagcc ttcatagcgt
    10321 ggcatttctt tttaccagaa taattcgcta attctttttt agggcgattg atttgtactt
    10381 ccgtcgcatc aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaacac
    10441 cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa
    10501 taccaaaatc agccgcaatt tgttcataag ttcgatattc tcgcacatat tgaagagtgg
    10561 ccataagaag gtcttctagg tttaatttag gttttcgtcc accttttgcg tgtttaagtt
    10621 gataagctgt ttttaataca gctaacatct cttcaaaagt ggtacgctga acactaacaa
    10681 gacgcttaaa ttgtgcatca gttagttgtt tacttgcttc ataattcata gaactactat
    10741 accatatttt gtttcgtagg aagtctaata agttatagtc cagtttttac tatactttga
    10801 tattcaaata tgttatacta tgattatcaa agtaaatgaa aggagttacg aatatgacgc
    10861 cagaagaaat gtacctgaca gagcgattag acgtacagat agctcatttt ttaaagaaaa
    10921 gcgttcaaca tcgtaggcgc tataaggtat taaaaataac agaaatcgtg gcaggtttcc
    10981 tcatagctgt cttttgtgct attcctatgc caggtgatcg ctaccgtttg atttcggttg
    11041 ccttatccag tctcggcttg ctgtgtgagg ggattatcaa tttgtataat gcaaaggaaa
    11101 attggatttc ttaccaaaaa actgcgcaac tcctggagaa agaaaaattc ctctatcaat
    11161 gccaaacgga gaaatatgca ggaaagacca aggcttttgc cctatttgtc aagacatgcg
    11221 aaggtcttat ctcagaggag attaaccagt gggaaagtat ccagtcaaaa gaagtggcag
    11281 ctagtgcaga tgctccagtt aaaaaagagt aggaggtaga ggaaatgtct caatccagct
    11341 acctgtcgcc cttgctctgg ttaaaaaaag aagccgataa ggaaaagatg agcgcgaccc
    11401 agtgccagat atttttcttt tactatcaaa tgtttgagct cttatttgct agagaaagcg
    11461 acatgaaaga cttatgtctg ggaacgaaag gtttttattt ctcgcagtta gagaaaaatt
    11521 tgctttctgg agtttcccga tttctaaaaa acttggaggg gaaagtaact ctcaaggcta
    11581 accaagaagt atcagctcgc aaagcccttt ttctagcctt gacaactagc caatcagatt
    11641 ggcaggagtt agctcctgtt tttgattttt atcagactat cgggaggctt gaaaatcctt
    11701 ctctcttgag ttctcaggac agacaacatc tgatgtggat ttaccagtca gctttggaga
    11761 aggattatat tgtcaaggtt attggcgaca agcattttgt attgaagaga caagatgcta
    11821 ctaaattgac agcgcgccaa actcaaactt tggaaattct gagtcaatca gaagacttgg
    11881 tcaatcctgt ctatgttaca ttaggagaaa agggggtgct cttgcttgat taagagagga
    11941 gatgttgtag ttctttattt gccttttccg actattagta gcgatttggc tgtgaagaat
    12001 catatgtata tctgtattga caacagcatg actaaaaaca aagagttggt taaaaatcag
    12061 accttcaaac cagctctctt gaccagacgt ttggtcaaga actttatgat agaagagccg
    12121 gatctagctc gtaatccttt tacaagacca accttgattg acttagataa ggtatttatg
    12181 ttggataata cggttattcc gacttcttat ctagccagac ggcgacgcaa tgtctcagaa
    12241 gaattgtacg aggaaatttt ggatcactta gtccaaccac ggctgatttc gctgaacaag
    12301 tctgagttta tgcaactcaa tccaggaact tattaggagg tagaagatgg caaataaagc
    12361 agtaaatgac tttatactag ctatgaatta cgataaaaag aaactcttga cccatcaggg
    12421 agaaagtatt gaaaatcgtt tcatcaaaga gggtaatcag ctacccgatg agtttgttgt
    12481 tatcgaaaga aagaagcgga gcttgtcgac aaatacaagt gatatttctg taacagctac
    12541 caacgacagt cgcctctatc ctggagcact tctcgtagtg gatgagacct tgttagagaa
    12601 taatcccact cttcttgcgg tcgatcgtgc tccgatgact tatagtattg atttgcctgg
    12661 tttggcaagt agcgatagct ttctccaagt ggaagacccc agcaattcaa gtgttcgcgg
    12721 agcggtaaac gatttgttgg ctaagtggca tcaagattat ggtcaggtca ataatgtccc
    12781 agctagaatg cagtatgaaa aaatcacggc tcacagcatg gaacaactca aggtcaagtt
    12841 tggttctgac tttgaaaaga cagggaattc tcttgatatt gattttaact ctgtccattc
    12901 aggcgaaaag cagattcaga ttgttaattt taagcagatt tattatacag tcagcgtaga
    12961 tgctgttaaa aatccaggag atgtgtttca agatactgta acggtagagg atttaaaaca
    13021 gagaggaatt tctgcagagc gtcctttggt ctatatttcg agtgttgctt atgggcgcca
    13081 agtctatctc aagttggaaa ccacgagtaa gagtgatgaa gtagaggctg cttttgaagc
    13141 tttgataaaa ggagtcaagg tagctcctca gacagagtgg aagcagattt tggacaatac
    13201 agaagtgaag gcggttattt tagggggcga cccaagttcg ggtgcccgag ttgtaacagg
    13261 caaggtggac atggtagagg acttgattca agaaggcagt cgctttacag cagatcatcc
    13321 aggcttgccg atttcctata caacttcttt tttacgtgac aatgtagttg cgacctttca
    13381 aaatagtaca gactatgttg agactaaggt tacagcttac agaaacggag atttactgct
    13441 ggatcatagt ggtgcctatg ttgcccaata ttatattact tgggatgaat tatcctatga
    13501 tcatcaaggt aaggaagtct tgactcctaa ggcttgggac agaaatgggc aggatttgac
    13561 ggctcacttt accactagta ttcctttaaa agggaatgtt cgtaatctct ctgtcaaaat
    13621 tagagagtgt accgggcttg cctgggaatg gtggcgtacg gtttatgaaa aaaccgattt
    13681 gccactagtg cgtaagcgga cgatttctat ttggggaaca actctctatc ctcaggtaga
    13741 agataaggta gaaaatgact aggagaggag aatgcttgcg acaaaaagag gcgatgatct
    13801 ctctgcggat atctgaaagt ttatctcttg cctagcgatt tccattggag tatgctcgtg
    13861 atgttaaaat gaatctatca aacagaatgt gtaaaaaagt aaaaagggta gccaattcct
    13921 tgatatacag gggattgatt atcctttact tgttttagat gattggaaat tcaagaatga
    13981 cacgtatcgg aaatattttg ctgtttatat ctaataccca tttatggaaa aaatgaaatt
    14041 aaatatgaac aaatcaattg ctagcaatgg tttagaagtg ccagtgtact agtctaattt
    14101 ctccatcttg ggaacacgta aattatactc ttcgaaaatc tcttcaaacc aggtcagccc
    14161 tatccgcaac ctcaaaacag tgttttgagc aacctgcggc tagctttcta gtttgctttt
    14221 tgatttttat tgagtattag aggtttgctt agaaactctt ctattgattt cttggaatac
    14281 aatagcgcct ataagaactg ttggatttca aagtatttac ttgaactatg atatgtctgg
    14341 agtagactgt caacaaaagg tgcagtatga ttgtttttgt cgcttatatc tacaaacttc
    14401 aaacagtaat ttgagctgac tttgtcaaac ttggctgaaa ctttctattt gcagataatg
    14461 gtctagtttg ttttttgaat gtggatgagt gtctgttgat aaaaagatag aaaggcaaga
    14521 ccatcttagc aggtaaagtg ctatcctagc ttgatttgct aataaaatcc atttcctcta
    14581 gtgaaaatca aaaaaacttg tgttataata agaaagatta aaatgtgaaa aaaggagatt
    14641 cctaatggga cgtaaatggg ccaatatcgt agccaagaaa acggctaaag atggagccaa
    14701 ctctaaagta tatgcaaaat ttggtgtaga aatctatgta gcagctaaaa aaggtgatcc
    14761 agatccagaa tcaaactcag ctttgaaatt cgttatcgac cgtgctaaac aagcccaagt
    14821 gccaaaacac attatcgata aagcgattga taaagccaaa ggaaacacag acgaaacctt
    14881 tacagaagga cgttacgaag gttttgggcc aaatggctct atgctaattg tggatacttt
    14941 gacttctaac gtcaaccgta cagcagccaa tgtccgtgca gcctttggta aaaacggcgg
    15001 aaacatgggc gcttcaggtt ctgtttctta cctctttgac aacaaaggtg ttattgtatt
    15061 tggaggtgaa gatgcggacg cagtctttga gcaattgctc gaagcggatg tggatgtgga
    15121 tgacgtagaa gcacaagaag gtacaatcac agtttacaca gcgccaactg accttcacaa
    15181 ggctatcgtt gccctccgtg agtctggtat tgaagaattc caagtgactg aattggaaat
    15241 gattcctcag tcagaagtgg aattgtcagg cgaagacctt gaaacctttg aaaaacttta
    15301 cagcgttctt gaagacgacg aagacgtcca aaagatttat acgaacgtag atgggtttta
    15361 atttaaccta tagaacatga tcctaagtga gaaaat
//
LOCUS       DAVMHG010000040        14681 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMHG010000040 DAVMHG010000000
VERSION     DAVMHG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.040"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..665
                     /locus_tag="VYR08_000996"
     CDS             <1..665
                     /locus_tag="VYR08_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEV3466540.1"
                     /translation="SACMMLQEGVSLKFSHRSFQEYFAAVGVNQLDDKLQKQILIKWS
                     EADRNNIVAHKTFMNALFKIQKERTYKNLCIPIIERMDEKYSRMGDITEKIVTCFNYF
                     ICRKDSRENKLELHFLLTKEVHFYFSLQFLIFANLGIDILSISDYENIEKLESDIVSN
                     WKINERKYYNMLNDDEKTLINKWINGWYFSRHNYLKEWSIKFIEETTTQKRSLQNIID
                     SI"
     gene            689..1786
                     /locus_tag="VYR08_000997"
     CDS             689..1786
                     /locus_tag="VYR08_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV3466541.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIADLYTEQGAEMENFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGYNIKLHPIQRQGAGYHSVDKDVNFASATALRQHQRDKDFLERFMPSVALFEQA
                     SKVSWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQVRESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            2024..2278
                     /locus_tag="VYR08_000998"
     CDS             2024..2278
                     /locus_tag="VYR08_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV3466542.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            2282..2536
                     /locus_tag="VYR08_000999"
     CDS             2282..2536
                     /locus_tag="VYR08_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV3466543.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            2727..4340
                     /locus_tag="VYR08_001000"
     CDS             2727..4340
                     /locus_tag="VYR08_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV3466544.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            4470..5096
                     /gene="gmk"
                     /locus_tag="VYR08_001001"
     CDS             4470..5096
                     /gene="gmk"
                     /locus_tag="VYR08_001001"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV3466545.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            5121..5435
                     /locus_tag="VYR08_001002"
     CDS             5121..5435
                     /locus_tag="VYR08_001002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV3466546.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            5501..7897
                     /locus_tag="VYR08_001003"
     CDS             5501..7897
                     /locus_tag="VYR08_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV3466547.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRTYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            7910..8845
                     /locus_tag="VYR08_001004"
     CDS             7910..8845
                     /locus_tag="VYR08_001004"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV3466548.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEVLPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            8838..10151
                     /gene="rsmB"
                     /locus_tag="VYR08_001005"
     CDS             8838..10151
                     /gene="rsmB"
                     /locus_tag="VYR08_001005"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV3466549.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            10166..10906
                     /locus_tag="VYR08_001006"
     CDS             10166..10906
                     /locus_tag="VYR08_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV3466550.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            10903..12882
                     /gene="pknB"
                     /locus_tag="VYR08_001007"
     CDS             10903..12882
                     /gene="pknB"
                     /locus_tag="VYR08_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV3466551.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            12997..13755
                     /locus_tag="VYR08_001008"
     CDS             12997..13755
                     /locus_tag="VYR08_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3466552.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            13757..14206
                     /locus_tag="VYR08_001009"
     CDS             13757..14206
                     /locus_tag="VYR08_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV3466553.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            14268..14681
                     /locus_tag="VYR08_001010"
                     /pseudo
     CDS             14268..14681
                     /locus_tag="VYR08_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4434 a         2855 c         3455 g         3937 t
ORIGIN      
        1 atagtgcttg tatgatgctt caagaaggcg ttagtttaaa attctctcac cgttcattcc
       61 aagaatactt tgctgcagta ggtgttaatc agctagatga taaactccaa aaacagatac
      121 taattaagtg gtctgaagca gataggaata atattgtagc acataaaact tttatgaatg
      181 cactttttaa aatacagaaa gaaaggactt acaaaaattt atgtattcca atcatagaaa
      241 gaatggatga aaagtatagc agaatggggg atatcacaga gaaaattgtt acgtgtttca
      301 attactttat ctgtagaaaa gatagtagag aaaacaaatt agaattgcac tttttactaa
      361 caaaggaagt tcacttttat tttagcttgc aatttttaat atttgctaat ctaggtattg
      421 atatattatc aataagtgat tatgaaaata tagaaaaatt agagtctgat atagtatcaa
      481 attggaagat aaatgaaaga aaatattata acatgttgaa cgatgatgaa aaaactttaa
      541 taaataagtg gataaatggc tggtatttta gtagacacaa ttacttaaaa gaatggtcaa
      601 ttaaattcat tgaggaaaca actactcaaa aacggagcct acagaatata attgactcaa
      661 tttgatacta aagaagtagg agattactat gaccatcacg ggtattatcg cggagttcaa
      721 tccttttcat aatggacaca aatacctgct ggatcaagcg gaggggctga aaatcgtggc
      781 tatgtcaggg aatttcatgc agcgtggaga acctgctatc gtggataagt ggacacgagc
      841 ccagatggcg ctggaaaatg gagcggatct ggtagtggaa ttgccctttt tagtcagcgt
      901 tcaggcggca gattttttcg gtcaaggggc cgtggatatc ttagctcggt tgggcataga
      961 tagcctcgct tttgggacag aagaagttct agattatcaa aaaatcgctg acttatacac
     1021 agagcagggg gctgagatgg agaattttgt ggaaaatttg cctgactctc tttcttatcc
     1081 ccagaagacc caagccatgt ggaaggaatt tgcaggtctt gatttttcag gcaatacgcc
     1141 caatcatgtt cttgccctcg cttacgccaa ggcggttgct gggtacaaca tcaaacttca
     1201 tccgattcag cgtcaggggg caggctacca ttctgtggac aaggatgtga actttgcttc
     1261 ggcgacagcc cttcgtcagc accagaggga caaagatttc ttagaacgat ttatgccttc
     1321 tgttgccctc tttgagcagg ccagtaaggt gagctgggaa gactattttc ccttgctccg
     1381 ctatcaaatc ttgtcaaatc ccgacctaac caccatctat caggtcaatc aagaaatggc
     1441 tgtgcgcatt aaagaagcta ttaaaacagc ccagtctgtg gaagaattgg tcgagttagt
     1501 gactaccaaa cgttacacca aggcgcgtgt cagacgccta ttgacctata ttttggtgca
     1561 ggttagagaa agtaacttgc cagaagcgat tcatgtcctt ggatttactg aaaaaggcag
     1621 gcaacatctc aagtctttga aaggtcaggt cagtctagtc agccgaattg gcaaagaacc
     1681 ttgggatgct atgacccaaa aagcagacca gatttaccaa ctaggaaagc caagtatagc
     1741 agagcaaaat tttggaagag tgccgattag aatagaaaca aactaagtct accgaaaggt
     1801 aggctttttg agatttttta cgaataatac tcttcgaaaa tcaaattcaa atcgcgtcaa
     1861 cgtcgccttg ccgtactcaa gtacagcctg cggctagttt cctagtttgc tctttgattt
     1921 tcattgagta taaatagata gaaaagaaaa tcttgtacaa gttcctgaca atttctttct
     1981 tttcgtgtat aatagtggaa aagagctaaa acgaggtatg gttatggaag cagtccttta
     2041 ctcaacattc cgaaatcatt taaaggacta catgaagaag gtaaatgatg aatttgagcc
     2101 tttgacggtg gtcaataaaa atccagatga ggacattgta gttctttcaa agagcgaatg
     2161 ggacagtatt caggaaaccc tgagaatcgc ccaaaataag gagttatcag acaaggtttt
     2221 gcgaggaatg gctcaagtcc gtgctggaag cactcaggtc catgtgattg aggagtgatg
     2281 aatgctactc aagtttacag aagatgcctg ggcagattat tgctactggc aaaaccaaga
     2341 taaaaaaaca ctaaaaagaa tcaataaatt aatcaaggat attcaacgcg atccatttac
     2401 aggaatagga aaaccagagc cactcaaata tgattaccaa ggagcctggt cacggcgtat
     2461 tgatgcagaa aatcgcttga tttatatgat ggatggagat agcgtggctt tcttgtcctt
     2521 taaagatcat tactaagtct actgcaaagt aggctttttc tcaagtctct actaaactac
     2581 tatttctaaa caacaagttt attttggtaa atgtattcca aattccccaa aacatattct
     2641 attttaggtt tgtgaaaatc acttttttat ggtagaatgt aaaagaatgt atgtcattcg
     2701 gaataacaat aaaatgaggt aaaaacatgg aaatcatgtc gcttgcgatt gctgtttttg
     2761 ccgtcatcat tggtttagtc attggatatg tcagcatctc agctaagatg aaatcatctc
     2821 aggaagctgc ggagttgatg cttttaaatg ctgaacaaga agcaactaat ttacgtggac
     2881 aagctgagcg tgaagcggat ttacttgtta atgaagctaa acgtgaaagc aagtctctta
     2941 aaaaagaagc actattggag gccaaagaag aagccagaaa ataccgtgaa gaagtggacg
     3001 ctgaattcaa atcagaacgt caagaactca aacaaatcga aagtcgtttg acagagagag
     3061 ctactagcct tgaccgtaag gacgacaatt tgacgagtaa agaacaaaca cttgaacaaa
     3121 aagaacaaag tatttctgat agagcgaaaa accttgatgc gcgtgaagag caattagagg
     3181 aagtcgaaag acaaaaagaa gcagaactag agcgtattgg tgctctgtct caggcagaag
     3241 cacgagatat tatcttggct cagacagagg aaaacttgac cagggagatt gccagtcgca
     3301 ttcgcgaagc tgagcaagag gtcaaggaac gttctgacaa aatggccaag gacatcttgg
     3361 ttcaagctat gcaacgtatc gctggtgaat atgtagcgga gtcaacaaac tcaacagttc
     3421 atctgccaga cgatactatg aagggacgca ttattggtcg tgaaggtcgt aacattcgta
     3481 cctttgaaag tttgacaggg gtcgatgtga ttatcgacga tacaccagaa gtggtgacct
     3541 tgtcaggatt tgatccgatt cgtcgtgaga ttgcccgtat gactatggaa atgttgctca
     3601 aagatggtcg tatacatcca gctcgtatcg aagagttggt tgagaaaaac cgtcaagaga
     3661 ttgacaataa gattcgtgaa tacggtgagg ctgctgctta tgaaattggt gcgccaaacc
     3721 ttcatccaga cttgatgaag attatgggac gtttgcagtt ccgtacttca tatggacaaa
     3781 atgttttgcg ccattcgatt gaggttgcta agttggctgg tatcatggcg agcgaacttg
     3841 gtgaaaatgc ggctcttgcc cgtcgtgctg gattccttca cgatatcggg aaagccattg
     3901 accatgaggt tgaaggtagc cacgttgaaa tcggtatgga attggcccgt aagtacaagg
     3961 aacccccagt tgtggtgaat acgattgcta gtcaccacgg agatgttgaa gctgagagcg
     4021 tgatagcagt tatcgtcgct gcagcagatg ccttgagcgc agcccgtcca ggtgctcgta
     4081 gtgagtctct tgaaagctac atcaagcgtc tccatgattt ggaagaaatt gctaacggct
     4141 ttgaaggagt gcaaactagc tttgcccttc aagcaggacg tgaaattcgt atcatggtca
     4201 atccaggaaa aatcaaggac gacaaagtca caatcttggc tcacaaagtt cgtaagaaaa
     4261 ttgaaaacaa tctcgattat ccaggaaata tcaaggtaac cgtgattcgc gagcttcgtg
     4321 cagtagatta tgctaaataa ataagaaaga gcagttgaaa aattactgct tttttgttac
     4381 actagataga aagactgtaa aggataatct ggctttttgc cattattcta gagaaatgtt
     4441 atttcacaga ttgactaaaa ggagacacaa tggcagaccg aggcttacta atcgtttttt
     4501 ctggtccttc aggggttgga aaaggaacgg ttagaagaga gatttttgag agttctgaaa
     4561 accaatttca atattctgta tcgatgacga cacgcgcaca acgtcctgga gaagtggacg
     4621 gtgttgacta tttcttccgt actcgtgaag aatttgaaga gctgattcgt caaggacaga
     4681 tgttggaata cgcagaatat gtcggcaact actatggaac tcctctgacc tatgtcaatg
     4741 aaaccttgga caagggaatc gatgttttcc ttgaaattga agttcagggt gctcttcagg
     4801 tcaagaaaaa ggttccagat gctgtcttta tcttcctgac accaccagat ttggatgaat
     4861 tgcaagatcg cttggtaggt cgtggaacag atagtgcaga agtgattgcc caacgaatcg
     4921 aaaaggccaa ggaagaaatt gccctcatgc gtgagtatga ttatgcgatt gtcaacgatc
     4981 aggtgcccct agctgctgaa cgtgtcaaat gtgtgattga agcagaacac ttctgtgtgg
     5041 atcgtgtcat tggtcactat caggagatgt taccaaaatc tccaactacc cgataaaatt
     5101 tagaaaatag gtacaagcaa atgatgttaa aaccctctat tgataccttg ctcgacaagg
     5161 ttccttcaaa atattcactc gtaatcttgg aagcaaaacg tgcccacgaa ttggaagcag
     5221 gtgccccagc aactcaaggt ttcaagtctg aaaaatcaac tcttcgcgct ttagaagaaa
     5281 tcgaatcagg aaacgttaca attcacccag atccagaagg aaaacgtgaa gcagtgcgtc
     5341 gccgtatcga agaagaaaaa cgccgcaaag aagaagaaga aaagaaaatc aaagagcaaa
     5401 ttgctaaaga aaaagaagat ggtgaaaaaa tttaaggttg gggggactca atcttatttt
     5461 ttctattgca agaatgtact gacaaggagg aggtgagaag atggccctag ctaagattat
     5521 cgtagatgtg cccttgatgc agacggacca gccctatagt tacaggattc cggaggaatt
     5581 tgagggaatg ctggaagttg ggatgcgggt tcatgtgcct tttggtaagg gtaatcgcct
     5641 gattcaaggg attgttcttg gtttgaagtc ccaatcagat ggagaagaga tggagcaaga
     5701 tttaaaagat attgcagagg tgctggattt ttctcctgtt ctcacgccag aacaactctg
     5761 gctggctgag gagttacgca aatctgtctt ctcctacaaa atttctatcc tcaaggccat
     5821 gcttccagga tttctgaatt ctagctatga caagattctc tatcctctgg aaggcttgag
     5881 tcaggaagaa cgagtgcgcc tgtttggttc agaagattcg ctagcctttt cttcactaga
     5941 ccttgccaag caagctgaaa tgatgcgttt gactagaaaa ggcctgcttg gtctggaata
     6001 tcaggcagtc gatcaaaaga aggtcaagac ccagtcttgg tatgaggttg accatgctca
     6061 attagaaggt gttgagattt ctacacgtgc caagaaaaag ttggaactga gagattatct
     6121 gctgtctcat ccagagagcg cttccttggc tagcttgtta gagtcctact cgcgagagca
     6181 agtcaacttc tttgtggatc aaggtgctgt taccatagtc caaaaggaag ttcaacgctc
     6241 ggctgcttat tttgaaggaa ttgaagcaag tagacctttg gagttaaatc cagaacaaag
     6301 acaggcgcgt gatgcggttg tgagttctat tggcagttct caacctccct ttctacttca
     6361 agggattaca ggaagtggga agaccgaggt ttacttgcag attatccaag gtgccctgga
     6421 taagggcaag acagctattt tgctggtacc tgagatttcc ttgactcctc agatgaccga
     6481 gcgttttata gcacgttttg gggacaaggt agccattctt cactcaggat tatccaatgg
     6541 tgaaaagtat gatgaatggc gcaaggtgga gcgtggcgat gcccaagttg ttgttggtgc
     6601 cagatctgct atctttgctc ctctgaaaaa tctgggtgtc atgattattg atgaagagca
     6661 tgaagtggct tataagcagg acagcaatcc ccgttaccat gccagagagg tagctatttt
     6721 acgggctcag tataatcaag caactctggt acttggatct gcaacgccga gcttagagag
     6781 ccgggcgcgt gctggcaaag gcgtctatca acacttacgt ctaacccaac gtgccaatcc
     6841 tttggctaca atccctgagg ttcaagtgat tgactttcga gactatatcg gacaaaatga
     6901 gacgtcaaac tttacgcctc ctttgctaga ggctatccaa gaccgtctgg ttaaaaaaga
     6961 gcaggtggtt ctcatgctca atcgtcgtgg ttattctagc tttgtcatgt gtcgggagtg
     7021 tgggacggtg gatacttgtc ccaactgcga tatttctctg accttgcata tggataccaa
     7081 gactatgaac tgccattatt gtggtttctc gaaggatatt cctcaggtct gtcctaactg
     7141 taagagccgc agtattcgtt actatgggac gggaactcag aaggcttatg atgagctagc
     7201 agaactcttt ccccaagctc gcattctgag gatggatgtg gatacgactc gaaagaaagg
     7261 cagtcaccaa gccttgcttg accagtttgg gcgaggggaa gcggatattt tacttggtac
     7321 tcagatgatt gcaaagggtt tggattttcc caatgttacc ctagtcggag ttctaaatgc
     7381 ggatacggcc ttgaatctgc ctgatttccg ttcttctgag agaaccttcc agctcttgac
     7441 tcaggtggca ggtcgagcag gtcgggctga aaaagcaggt caagtcttga tacagtccta
     7501 caatccgcag cactatgcta ttcgatttgc caaggatcaa gattacgaag gcttttatgc
     7561 ctatgaaatg ggaatcagac gacaactcgg ctatccacct tactatttca cgattggtat
     7621 taccctttct cacaagaaag aagaagaggt tttcaaacgt acctatgaag tcatgaacat
     7681 tttgcggtca ggcttgtcag agaccagtcc cattctgggg ccaacgccaa aaccgattgc
     7741 ccgtacccac aacctctatc attaccagat tttaattaaa taccgtttag aagatgagct
     7801 ggggccgacc ctcaaccagg tcctggcctt gactcaagaa cgggaaaata gtgagctccg
     7861 tctcagcatt gaccatgagc cgcagcaatt tttataagaa ggagaagata tgacaaaact
     7921 aatctttatg gggacccccg acttttcagc aacagtctta aaaggacttt tgacagatga
     7981 ccgttacgaa attctagccg ttgtgaccca gccagaccgt gctgttggtc gtaaaaaagt
     8041 tatccaagaa accccagtca agcaggctgc caaggaagca ggactatcta tctaccaacc
     8101 tgaaaaatta tctggaagtc cagagatgga agatcttatg aagctaggag cagatggaat
     8161 tgtgactgct gcttttgggc agtttctccc aagcaaactc cttgatagca tggactttgc
     8221 tgtcaacgtt catgcctccc tccttcctag acaccgtggt ggtgcgccta tccattatgc
     8281 cttgattcaa ggggatgagg aagctggtgt gaccatcatg gaaatggtta aggaaatgga
     8341 tgcaggagat atgatttctc gtcgcagcat tccgattaca gatgaggaca atgttggcac
     8401 cttgtttgaa aaattggcgc tagttggttg tgatttgctt ttggacactc tgcctgccta
     8461 tattgctggt gatatcaaac ctgaaccgca ggatacgagt caggttacct tctctccaaa
     8521 tataaagcca aaggaagaaa aactggactg gaacaaaacc aatcgtcaac tctttaacca
     8581 aattcgtgga atgaacccct ggcctgttgc ccatactttc cttaagggcg accgctttaa
     8641 gatttatgaa gtcctaccag tagaaggtca gggaaatcca ggtgagattc tctctatcgg
     8701 caagaaagaa ttgattgtcg caacggctga aggggctcta tccctcaaac aagtgcagcc
     8761 agctggtaag cctaagatgg acattgcttc cttcctcaat ggagttggac gtacattgac
     8821 tgtaggagaa cgatttggtg actaaagtag aaacggctag aagtttagct ctagcagtgc
     8881 tagaggatgt ttttgtgaat caagcatatt caaatattgc cttaaataaa cacctcaagg
     8941 ggagtcagct ctctgcagca gacaagggct tagtgaccga gctagtctat ggaacggtag
     9001 cccgtaaact gactctggaa tggtacctat cccactttat cgaagacaga gaccagttag
     9061 acagctggct ctatgtcctt cttctcatga gtgcctacca actccgctat ttggacaaga
     9121 ttccagatca tgctgtggtc aatgaagcag tggaattagc caaactccgt aaaaaaggca
     9181 gtgaaaaatt ggtcaacgct gtccttcgcc gtatcttgcg tgaaggctgg ccagatattg
     9241 ctagcatcaa gcgaaaaaac aagcgtgact ccattgccta ttctctccca gtttggctag
     9301 ttgccaaact caaggaagaa tacggagagg agagagcaaa agccatcttt gaaagcctgt
     9361 tagtgcgaaa caaagccagt attcgtgtaa cagacctaag tcgaaaagag gaaatccaag
     9421 ccttgttgga ggcgaatgat tcccttttag caaccactgg tctggttaag gagcaggggc
     9481 attttgcagg gcataatttg tttgcggatg gagccattac catccaagac gagtccagtc
     9541 agctggttgc tccgacgctt gatttacaag gtgatgagcg agtgcttgat gcctgtgcgg
     9601 ctccaggtgg gaaaacagcc catatagcct cttatcttac gacaggtcag gttactgctc
     9661 tggacttgta cgaccacaag ttggatttaa ttcaagaaaa tgcccaacgt ctgggagttg
     9721 cagatcgggt tcaaactcaa aaattggatg ccagaaaggt gcatgagttt tttgaccagg
     9781 attcctttga taagattttg gtggatgctc cttgttcagg aatcggtctt ttgcgccgaa
     9841 aaccagatat caaatacaat aaagaaacgg cagatttcgc gtccttgcag gaaattcagt
     9901 tagaaatatt aggtagtgtt tgtcaaatac taggcaaagg tggtataata acttatagca
     9961 cctgcactat cgtctcagag gagaattttc aagtcgttaa ggccttttta gagagtcatc
    10021 ccgagttcga gcaggtaaaa ctagaacatg aatgtaagga tatcatgaaa gacggctgta
    10081 tcctcattac acctgaattg tatggaagtg atggattctt catcagtcaa tttcgcaaga
    10141 tatcggatta ggaaggaact gacacatgga aatttcatta ttaacagatg ttggtcagaa
    10201 acgaacaaat aaccaagact atgtcaacca ctatgtcaat agagctggac gtaccatgat
    10261 tattttagct gatgggatgg gaggtcatcg cgcagggaat atcgctagtg aaatggcggt
    10321 cacagacctg ggtgtagctt gggttgatac ccagattgat acagtcaatg aagtgcgtga
    10381 atggttcgcc cattacctag aaattgaaaa tcaaaagatt caccagcttg gtcaggatga
    10441 agcttacaga ggcatgggaa ctactttgga agtccttgct attattgata atcaggctat
    10501 ctatgctcat attggtgatt cgcgtatcgg cttgattcgt ggagaagaat accatcagtt
    10561 gacgagcgat cattctttgg ttaatgaatt gctcaaggct ggtcaattga caccagaaga
    10621 ggcagaagct catccacaaa aaaatattat cacccagtct attgggcaaa aagatgaaat
    10681 tcagcctgat tttgggacag ttatccttga gtcaggtgac tatctcttgc tcaatagtga
    10741 cggcttgacc aacatgattt caggcagtga gattcgtgat attgtaacca gtgatattcc
    10801 tttagcagat aaaacggaga cacttgttcg ttttgctaac aatgcaggag gtttagacaa
    10861 cattacggtt gcccttgttt ctatgaacga ggaggatgca gaatgatcca aatcggcaag
    10921 atttttgccg gacgctatcg gattgtcaaa cagattggtc gaggaggcat ggcggatgtc
    10981 tacctagcca aagacttaat cttagatggg gaagaagtgg cagtgaaggt tctgaggacc
    11041 aactaccaga cggacccgat agctgtagct cgttttcagc gtgaagcgag agctatggca
    11101 gatctagacc atcctcatat cgttcggata acagatattg gtgaggaaga cggtcaacag
    11161 tatcttgcaa tggagtatgt tgctggacta gacctcaaac gctatatcaa ggaacattat
    11221 cctctttcta atgaagaagc agtccgtatc atgggacaaa ttctcttggc tatgcgcttg
    11281 gcccatactc gaggaattgt tcacagggac ttgaaacctc aaaatatcct tttgacacca
    11341 gatgggactg ccaaggtcac agactttggg attgctgtag cctttgcaga gacaagtctg
    11401 acccagacta actcgatgtt gggctcagtt cattacttgt caccagagca ggcgcgtggt
    11461 tcgaaggcga ctgtgcagag tgatatctat gccatgggga ttattttcta tgagatgttg
    11521 acaggccata tcccttatga cggggatagc gcggtgacca ttgccctcca gcatttccag
    11581 aaacccctgc cgtccgttat tgcagaaaat ccatctgtac ctcaggcttt agaaaatgtt
    11641 attatcaagg caactgctaa aaagttgacc aatcgctacc gctcggtttc agagatgtat
    11701 gtggacttgt ctagtagctt gtcctacaat cgtagaaatg aaagtaagtt aatctttgat
    11761 gaaacgagca aggcagatac caagaccttg ccgaaggttt ctcaaagtac cttgacatct
    11821 attcctaagg ttcaagcgca gacagaacac aaatcaatca aaaacccaag ccaggctgtg
    11881 acagaggaaa cttaccaacc acaagcaccg aaaaaacata gatttaagat gcgttacctg
    11941 attttgttgg ccagccttgt attggtggca gcttctctta tttggatact atccagaact
    12001 cctgcaacca ttgccattcc agatgtggca ggtcagacag ttgcagaggc caaggcaacg
    12061 ctcaaaaaag ccaattttga gattggtgag gagaagacag aggctagtga aaaggtggaa
    12121 gaagggcgga ttatccgtac agatcctggc gctggaactg gtcgaaaaga aggaacgaaa
    12181 atcaatctgg ttgtctcatc aggcaaacaa tccttccaaa ttagtaatta tgtcggccgg
    12241 aaatcttctg atgttatcgc ggaattaaaa gagaaaaaag ttccagataa tttgattaaa
    12301 attgaggaag aagagtcgaa tgagagtgag gctggaacgg tcttgaagca aagtctacca
    12361 gaaggtacga cctatgactt gagcaaggca actcaaattg ttttgacagt agctaaaaaa
    12421 gctacgacga ttcaattagg gaactatatt ggacggaact ctacagaagt aatctcagaa
    12481 ctcaagcaga agaaggttcc tgagaatttg attaagatag aggaagaaga gtccagcgaa
    12541 agcgaaccag gaacgattat gaaacaaagt ccaggtgccg gaacgactta tgatgtgagt
    12601 aaacctactc aaattgtctt gacagtagct aaaaaagtta caagtgttgc catgccgagt
    12661 tacattggtt ccagcttgga gtttactaag aacaatttga ttcaaattgt tgggattaag
    12721 gaagctaata tagaagttgt agaagtgacg acagcgcctg caggtagtgt agaaggcatg
    12781 gttgttgaac aaagtcctag agcaggtgaa aaggtagacc taaataagac tagagtcaag
    12841 atttcaatct acaaacctaa aacaacttca gctactcctt aaaagcagat ggataatcaa
    12901 aatgagtaat caatctttgt catgatttca tggcaaagat tttttttgag tccggatttg
    12961 tgatagaata gagggagttg ataaaaggag gaaagcatgg aagaatcaaa agaattaaat
    13021 gccgtcattg atgtgattat gctagcgggg actattctcc ttaaaagtgg ctcagaaatc
    13081 catcgtgtag aagataccat gattcgaatc gcgcattcgc aggggattgt ggattgcaat
    13141 gtccttgcca tgcctgccgc tatctttttc tctattgaaa ataccaatat ttcgcgcatg
    13201 aagcgcgtga cctcctcttc ttataacatc gaaaaagtct gcgatgtgaa ccagatttct
    13261 cgtcagctag ttggggggca gattgactta gaaacagcct ttaagcaatt gacggccttg
    13321 caagctcaac cccttcccta tactaagttg caggtaactc tggctgcgac ctttagtgct
    13381 cctttctttt cagttatgtt tagcggaaat atctacgacg cacttggggc aggagtggcg
    13441 accttatttg gttttgcctt ttccctctat gtggaaaaat ttatccgaat tccctttgtg
    13501 acagcctttg ctggagcctt tgtctttggg ataattgccc agttttgggc tcgctacaca
    13561 ggttttcctt caacggcaga tttgattata gctggtgcgg tcatgccgtt tgtaccaggt
    13621 attgccttga ccaatgcggt ccgtgatatt atgaccaacc acataaactc tggtatgagt
    13681 aagatgtttg aatccctgct cattaccctc gctttagggg caggaacttc tgtcgccttg
    13741 gtattgatga actaatatga cactaacaac ctttttatta caagcagtag caagttttct
    13801 tgccattatc acttttttaa tcgtactcaa tgtgcaacgg tctatgctct tacctggagg
    13861 gattttgggc atgactgtct ggctaatcta tctcttgctc aaggaaccga ccaatgtcat
    13921 tgtagctacc ttcattgcag ccattattgg ttcttgtgtc agccagattt taagtattct
    13981 ttataagaca cctgctgtgg tctttatctt ggccattttg gcaccgctgg ttccaggtta
    14041 tctctcctac cgaacaactg ccttttttgt gacaggggac tataataaag cactggcaag
    14101 tgcgaccttg gttgtcatgt tggctttggt aatctctatt ggaatggcta gcggaacagt
    14161 gattctcaga ctgtatcatt atataaaaac acatcgagta tcgtagactt tacagaaata
    14221 aaagaatttt ctgaaaaatg agataaataa attaacaacg ctttctatat gtgcgagaat
    14281 accgcactta tgaagaaatt gcggctgatt ttggtatcca cgaaagcaac ttactccgtc
    14341 ggagccaatg ggttgaagta actcttgttc aaaggtggtg ttacgatttc aagaactcct
    14401 ctcagttctg aagacacggt aatgattgat gcgacggaag taaaaatcaa tcgccctaaa
    14461 aaaaacaatt agcgaatgat tctggtaaaa agaaatttca cgctatgaag gctcaagcga
    14521 ttgtcacaag tcaagggaga attgtttctt tggatatcac tgtgaactat tgtcatgata
    14581 tgaagttgtt caaaatgagt cgcagaaata tcggacaagc tggtaaaatc ttggctgaca
    14641 gtggttatca aggacccatg aagatatatc ctcaagcaca a
//
LOCUS       DAVMHG010000041        14555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMHG010000041 DAVMHG010000000
VERSION     DAVMHG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.041"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            247..1152
                     /locus_tag="VYR08_001011"
     CDS             247..1152
                     /locus_tag="VYR08_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3466554.1"
                     /translation="MQSVAPLVTYLFVVAISILDGFAAPVSYAIVPRYATDLGKANSA
                     LSMTGEAVQLIGWGLGGLLFATIGLLPTTFIILVLYIISSFLMLFLPNAEVEVLESET
                     NLEILLKGWKLVARNPRLRLFVSANLLEIFSNTIWVSSIILVFVTELLNKTESYWGYS
                     NTAYSIGIIISGLIAFRLSEKFLAAKWESILFPLVAMAIVTLTILYFPNAQMFLLFSA
                     LVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVISTLAFSVFVLLMSYITESFGIS
                     ISFWLSAICLMIEAILIYIRRDYFK"
     gene            1466..1597
                     /locus_tag="VYR08_001012"
     CDS             1466..1597
                     /locus_tag="VYR08_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV3466555.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            1618..3168
                     /gene="dltA"
                     /locus_tag="VYR08_001013"
     CDS             1618..3168
                     /gene="dltA"
                     /locus_tag="VYR08_001013"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV3466556.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATSSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3165..4409
                     /gene="dltB"
                     /locus_tag="VYR08_001014"
     CDS             3165..4409
                     /gene="dltB"
                     /locus_tag="VYR08_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV3466557.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4423..4662
                     /gene="dltC"
                     /locus_tag="VYR08_001015"
     CDS             4423..4662
                     /gene="dltC"
                     /locus_tag="VYR08_001015"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV3466558.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            4655..5923
                     /gene="dltD"
                     /locus_tag="VYR08_001016"
     CDS             4655..5923
                     /gene="dltD"
                     /locus_tag="VYR08_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV3466559.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6078..6518
                     /gene="adcR"
                     /locus_tag="VYR08_001017"
     CDS             6078..6518
                     /gene="adcR"
                     /locus_tag="VYR08_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV3466560.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6518..7222
                     /locus_tag="VYR08_001018"
     CDS             6518..7222
                     /locus_tag="VYR08_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3466561.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7215..8021
                     /locus_tag="VYR08_001019"
     CDS             7215..8021
                     /locus_tag="VYR08_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3466562.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8031..9536
                     /gene="adcA"
                     /locus_tag="VYR08_001020"
     CDS             8031..9536
                     /gene="adcA"
                     /locus_tag="VYR08_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV3466563.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQSLDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKVLTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(10707..11480)
                     /locus_tag="VYR08_001021"
     CDS             complement(10707..11480)
                     /locus_tag="VYR08_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3466564.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            11656..13059
                     /locus_tag="VYR08_001022"
     CDS             11656..13059
                     /locus_tag="VYR08_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV3466565.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSTLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEDSLFISSGTWSLVGVELSSPILSTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETGEWTDGQLFKIVYESLAETYKETIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNICIQLLSQGKINEDTGLK
                     DIMTNIADTQYYYPQLS"
     gene            13088..14380
                     /locus_tag="VYR08_001023"
     CDS             13088..14380
                     /locus_tag="VYR08_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466566.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWEDFTQAMAAVRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
BASE COUNT         4408 a         2571 c         3067 g         4509 t
ORIGIN      
        1 gtctctcaat tatttgcaaa tttagcagat attttcttta gagtaacaat cattgctaac
       61 atatacatta tttcaaaatc agtaattgcc acatcactag ttcctatctt aataggaata
      121 tcctcttttg ttgcgagtct tttagttccg ttggttacta aaaggttagc gctaaatagg
      181 gttttatctt tatctcaatt tggaaagact atattattgg cgatactggt aggaatgttt
      241 accgtaatgc aatccgtagc gcctttggtg acctatctat ttgttgttgc aatttccata
      301 ctagatggtt ttgcagcacc cgtttcctat gctattgtgc cacgctatgc gaccgatttg
      361 ggtaaggcta attcagcctt atcaatgact ggtgaagctg ttcaattgat aggttgggga
      421 ttaggtggac tcttgtttgc aacaattggt ctgttaccta ccacgtttat cattttagtc
      481 ttgtatatca tttctagctt tctgatgtta tttcttccta acgctgaagt ggaggtgtta
      541 gagtcagaaa ctaatcttga aattttgctc aaaggttgga agttagttgc tagaaatcct
      601 agattaagac tttttgtatc agcaaattta ttggaaattt tttcaaatac gatttgggtt
      661 tcttccatta tacttgtttt tgtaacggag ttattaaata aaacggaaag ttactgggga
      721 tattctaata cagcatactc tattggtatt ataattagtg gcttaattgc ttttaggcta
      781 tctgaaaagt tccttgctgc taaatgggaa agtattcttt ttcctcttgt agctatggca
      841 atagtgacac tgactattct atattttcca aacgcacaga tgtttttatt attttcagct
      901 ttagttggta tgttatcgca actaaaagaa gttcctgaaa gtgtatttct tcaggaaaca
      961 gtagaggaaa atcatttagt taatgtctat tccgttcttg aagtgatttc tacattagca
     1021 ttttcagtat ttgttttgct aatgagctat attactgaga gttttggtat tagcatcagt
     1081 ttttggctat cagccatttg tctgatgata gaagctattt taatttatat cagacgagat
     1141 tattttaaat gagagagtgg ttaaaaagtc tttaaaaatc agaaaagcgc atagtatcag
     1201 gtgttgaaaa aacttgatat tgtgtgtttt attatgggaa gatttacttc attgtctcct
     1261 gaaatggagt tttgaagcag gatcactcta tttgaaaata tacatagaaa aaccgcataa
     1321 tccttaaaat aagctgaaat ctattggaaa catacggtaa acaattgaaa agatactata
     1381 taaagtggta caatggtaga aaaataagct agtaagaatc actcttattt tagcgaaaaa
     1441 ttacagaaat gaatggtttt tcttgatgaa acagagaaaa gaattgtacc tctttcttgg
     1501 tcggacagcc ttgtattttc ttatctttct agggctgctt tacttcttta gctatcttgg
     1561 tcagggtcaa ggaagcttta tctataatga attttaactt gaaggagaat ccctattgtg
     1621 tcaaataaac caatagcaga tatgattgaa accattgagc attttgctca gacacagcct
     1681 agctatcctg tctataatgt tttggggcag gaacacactt atggcgattt aaaggctgat
     1741 tcggatagtt tggctgcagt cattgaccaa ctaggcttgc ctgagaagtc tcctgtggtt
     1801 gtttttggtg gccaagaata tgaaatgttg gcaacctttg tagcgctgac taagtcaggt
     1861 catgcctaca ttccaattga tagccattcg gccttggagc gagtttcagc tattttagaa
     1921 gtagcagagc caagcttgat tattgccatt tcagcctttc ccttggagca ggtttctaca
     1981 ccaatgataa atctagctca ggttcaagaa gcctttgccc aagggaacaa ctatgaaatc
     2041 acgcatccag tcaagggaga tgataattac tacattatct ttacttctgg tacgactggt
     2101 aagcctaagg gagtgcagat ttcacatgat aatctcctca gctttacaaa ctggatgatt
     2161 acggataagg aatttgcgac atcgagtcgt ccgcaaatgc tggcacagcc accttattct
     2221 tttgacttgt ctgtcatgta ttgggcaccg accttggcac ttggtggtac gcttttcact
     2281 cttccttcag tcatcactca ggactttaag caactctttg cggctatctt ttcattgcca
     2341 atcgctatct ggacatcaac accatccttt gcagatatgg ccatgttgtc tgaatacttc
     2401 aacagtgaga aaatgcctgg aatcacgcat ttctactttg atggtgaaga attgacggtc
     2461 aaaacagctc aaaaactgcg cgagcgtttc ccaaatgccc gtatcatcaa tgcttacggc
     2521 ccaacagaag cgacagtagc tctgtcagca gttgccgtga cagacgagat gttagcgact
     2581 ctcaaacgcc taccaatcgg ctatactaag gctgattctc caacctttat cattgacgag
     2641 gaaggaaata aactgccaaa tggtgagcag ggagaaatca ttgtttctgg gccagctgtt
     2701 tcaaaaggtt atatgaacaa tcctgaaaaa acagcagaag ccttctttga gtttgaaggt
     2761 ctgccagcct atcacacagg cgatgtggga accatgacag atgagggctt gcttctctac
     2821 ggcggacgca tggacttcca gattaagttt aacggttacc gcattgagtt agaagatgtc
     2881 tctcaaaacc tcaacaagtc tcgctttatc gaatctgctg tcgcagtacc gcgctataac
     2941 aaggaccaca aggtacaaaa tctattggct tatgtcatct taaaagacgg tgttcgtgag
     3001 cagtttgagc gagatatcga tattaccaag gccatcaagg aagacctgac agacatcatg
     3061 atgtcctata tgatgccatc taaattcctt taccgagaca gtttgccact aactccaaat
     3121 ggaaagattg acatcaaagg attgattaac gaggtgaata agagatgatg gagttttttc
     3181 aacagcttcc tcatttagag ccatatggca atcctcagta ttttgtttat gtgattgctg
     3241 caaccttgcc catctttata ggtctctttt tcaagaaacg ctttgcctgg tatgaagtgt
     3301 tggtaagtct cttctttatt gtcaccatgt tggtgggtgg aaagaccaat caactagctg
     3361 ccttgggtat ttacctttgc tgggaaatat tgctcctgct tttctacaag cattatcgaa
     3421 aaagcaagga tggcaagtgg gtcttctact tagttagttt tctgtcccta cttccgatta
     3481 tctttgtcaa ggtgcaacca gctatcaatg gaacgcagtc tttgcttggg ttcttgggaa
     3541 tttcttacct tacctttcgt tcggttggaa ttgtcatcga gctgagagat ggagtgatta
     3601 aggattttac cctctgggaa ttcctccgtt tccttctctt catgccaact ttctcgagtg
     3661 gtccaatcga tcgctttaag cgatttaatg aaaattatca ggctattcct gagcgagatg
     3721 agttgatgga tatgctggat gaatctgtcc gctatatcat gtggggcttt ttgtataagt
     3781 ttatcctagc tcatgtttta ggagagacct tactacctcc tctgaagaat ttagccttgc
     3841 agtcaggtgg cttctttaat ctctatgcct tggcagttat gtatactttt ggtctggaac
     3901 tcttctttga ctttgcaggt tattctatgt ttgctttggc catctcaaac ttgatgggaa
     3961 tccgtagccc tatcaacttt aacaagccct ttttatcaag ggatttaaag gagttttgga
     4021 atcgctggca tatgagtctg tccttctggt tccgtgactt tgtctttatg cgaatggtga
     4081 tggtgttaac cagaaagaaa gtctttaaaa atcgtaatgt aacctcaagc atggcctaca
     4141 ttgtaaatat gctgattatg ggattttggc atggcgtgac ctggtactat atcgcctatg
     4201 gactctttca tggactaggc ttggtcatca atgatgcctg ggttcgcaag aaaaaaacgc
     4261 tcaataagga acggaaaaaa gcagggaagg ctgccctacc tgagaatcgc tggattcagt
     4321 ttcttggcat ggttgtcact ttccatgttg tcatgttgtc attcttaatc ttttctggat
     4381 tcttgaataa tctatggttt aaaaaataaa aggaaataaa atatggatat caaatcagaa
     4441 gttattgaaa ttattgatga gttgtttatg gaagatgttt ctgacatgat ggatgaagat
     4501 ctttttgatg caggtgtctt ggatagtatg ggaacggttg agttgattgt ggagattgag
     4561 aaccgttttg acattcgtgt ccctgtaaca gagtttggtc gtgacgactg gaatacagct
     4621 aataaaatca tagctggtat tgtggagcta caaaatgctt aaacgcttat ggatgatctt
     4681 cggaccggtc ttgatcgctg gtttgttggt ttttctgctc attttctttt atcctactga
     4741 gatgcatcat aatctaggag ctgaaaagcg ttcagcagtg gctactacta tcgatagttt
     4801 taaggagcga agtcaaaaag tcagagcact atctgatcca aatgtgcgtt ttgttccctt
     4861 ctttggctct agtgaatggc ttcgttttga cggtgctcat cctgcggtat tagctgagaa
     4921 atacaatcgt tcctaccgtc cttatctttt aggacagggg ggagctgcat cgcttaacca
     4981 atattttgga atgcaacaga tgttaccaca gctggagaat aaacaagttg tgtatgttat
     5041 ctcacctcag tggttcagta aaaatggcta tgatccagca gccttccagc agtattttaa
     5101 tggagaccag ttgactagtt ttctgaaaca tcaatctggg gatcaggcta gtcaatatgc
     5161 agcgactcgc ttactgcaac agttcccaaa cgtagctatg aaggacctgg ttcagaagtt
     5221 ggcaagtaaa gaagaattgt cgacagcaga caatgaaatg attgaattat tggctcgttt
     5281 taatgaacgc caagcctcct tttttggtca gttttcggtt agaggctatg ttaactacga
     5341 taagcatgta gctaagtatt taaaaatctt gccagaccag ttttcttatc aagcaataga
     5401 agatgttgtc aaagcagatg ctgaaaaaaa tacttccaat aatgagatgg gaatggaaaa
     5461 ttatttctat aatgagcaga tcaagaagga tttgaagaaa ttaaaggatt ctcagaaaag
     5521 ctttacctat ctcaagtcgc cagagtataa tgacttgcag ttggttttaa cacagttttc
     5581 taaatctaag gtaaacccga tttttatcat tccacctgtt aataaaaaat ggatggacta
     5641 tgctggtcta cgagaggata tgtaccaaca aacggtgcag aagattcgct accagttaga
     5701 aagtcaaggt tttaccaata tagcagattt ttctaaggac ggcggggagc ctttctttat
     5761 gaaggacacc attcaccttg gttggttggg ttggttggct tttgacaagg cagttgatcc
     5821 tttcctatcc aatcccacac cagctccgac ttaccatctg aatgagcgct ttttcagcaa
     5881 agattgggcg acttatgatg gagatgtcaa agaatttcaa tagataatat aatatagaag
     5941 agttcaagaa tgaaactagt tttcacgttt tttcttgact ctttttttgc ttgtgatata
     6001 attaactggt aaacaaaatg tcaaagaata gtatttttct cttaaaaaag agaagtctaa
     6061 aaggaaagga gtcagatatg agacagctag caaaggatat caatgctttt ttgaatgagg
     6121 tgattttgca ggcggaaaat cagcatgaaa tcctaatagg tcattgcact agcgaggtgg
     6181 ccctgaccaa tactcaggag catatcctta tgctcttgtc agaggaatct ttaacaaatt
     6241 cagaattggc ccgtcgtctc aatgtcagtc aggcggcagt taccaaggcc attaagtctt
     6301 tggtcaagga agggatgttg gaaacatcta aagattctaa agatgcgcgt gtgatttttt
     6361 atcagttgac tgacttggct cgtccaatcg ctgaggagca ccaccatcac catgagcata
     6421 cacttttaac ctatgaacaa gtggcgactc agtttactcc aaatgaacaa aaagtgattc
     6481 agcggttttt gactgcttta gtaggagaaa tcaaataatg agatatatta cggtagagga
     6541 tttgtccttc tattatgata aggagcctgt tcttgaacat atcaattata gtgttgatag
     6601 tggggaattt gttaccttga ctggggaaaa tggagcggct aagacgacgc tcatcaaggc
     6661 tagtcttgga attctgcaac cacgcattgg aaaggtggct atttcaaaga caaatacgca
     6721 aggtaagaaa ttgagaatag cctatcttcc tcaacaaatt gccagtttta atgctggttt
     6781 tccaagtacg gtctatgaat ttgtcaagtc gggtcgctat ccgagaaaag gctggttccg
     6841 tcgtttgaat gctcatgatg aggagcatat caaggctagt ctggactcag ttggcatgtg
     6901 ggaacatcga gacaaacgct tggggtctct atctggggga caaaagcagc gagcggtaat
     6961 tgcgcgtatg tttgcttctg accctgatgt gtttatccta gacgagccga caacggggat
     7021 ggatgcagga agtaaaaatg aattttacga actcatgcac cacagcgccc atcatcatgg
     7081 caaggctgtt ttgatgatta cccatgaccc tgaagaagtt aaggattatg cggatcgcaa
     7141 tattcatcta gtccgtaacc aagactcgcc atggcgttgt ttcaatgttc atgagaatgg
     7201 ccaggaggtg ggccatgctt agtttattat cttacgactt tatacagcgc gcctttttgg
     7261 cggttattgc tatgagtctt ttctcaccgg tattgggaac cttcctcatc ttgcgtcgtc
     7321 agagtttgat gagtgatacc cttagccacg tctcactttc aggtgtagcc tttggtctgg
     7381 ttttggggat ttctccaact gtttctacta ttgccattgt cttgattgcg gcggtctttc
     7441 tggagtatct ccgtacggtt tacaagagct ttatggaaat cgggacagct atcctcatgt
     7501 caacaggtct ggctgtttct ctgattgtca tgagcaaggg taaaagctcg agttcaatga
     7561 gtttggacca atatctcttt ggttcgatcg tgactatcag tgaagaacag gtcatttccc
     7621 tctttgttat tgcggcggtt gttttgattt tgacctttct ctttcttcgt cctatgtata
     7681 tcttaacttt tgacgaagat acggcctttg tggatggctt gccagttcgt accatgtcca
     7741 ttctttttaa catggtgaca ggggtggcta ttgcccttat gattcctgca gcaggagctc
     7801 ttctggtatc gaccattatg gtcttgccag ctagtattgc cctgcgtctg gggaaaaact
     7861 ttaaatcggt tatgctgctt gccagtgcga ttggcttttt gggaatggta gcaggacttt
     7921 acatttccta ctatgcagaa acacctgcaa gtgcaagtat taccattatt tttgtaactg
     7981 tctttatact aatcagttta gtaagacgtt ttatcaaata ggagacgaac gtgaaaaaaa
     8041 ttagcttatt actagccagt ctatgtgcct tgtttttagt ggcttgttcc aatcaaaaac
     8101 aggcagatgg taaactcaat atcgtgacaa ccttttaccc tgtctatgaa tttaccaagc
     8161 aagttgcagg agatacggct aatgtagaac tcctaatcgg tgctgggaca gaacctcatg
     8221 aatacgaacc atctgccaag gcagttgcca aaatccaaga tgcagatacc ttcgtttatg
     8281 aaaatgaaaa catggaaaca tgggtaccta aattgctaga taccttggat aagaaaaaag
     8341 tgaaaaccat caaggcgaca ggcgatatgt tgctcttgcc aggtggcgag gaagaagagg
     8401 gagaccatga ccatggagaa gaaggtcatc accatgagtt tgacccccat gtttggttat
     8461 caccagttcg tgccattaaa ctagtagagc acatccgcga cagcttgtca gcagattatc
     8521 ctgataaaaa agagaccttt gagaagaatg cagctgccta tatcgaaaaa ttgcaatcct
     8581 tggataaggc ttacgcagaa ggcttgtctc aagcaaaaca aaagagcttt gtgactcaac
     8641 acgcagcctt taactatctt gccttggact atggactcaa acaagtcgca atctcaggcc
     8701 tttctccaga tgcagagcca tcagctgctc gcttggcaga attgacagag tatgtcaaga
     8761 aaaataaaat cgcctatatc tattttgaag aaaatgcctc acaagccctt gctaacacac
     8821 tttcaaaaga agcaggtgtc aaaactgatg tcctcaatcc tttagaaagt ctgacagaag
     8881 aggacaccaa ggctggagaa aactacattt ccgtgatgga gaaaaacctc aaggctttga
     8941 aacaaacaac agaccaagaa ggcccagcaa tcgaacctga aaaggcagag gataccaaga
     9001 cagtccaaaa tggttacttc gaggatgcag ctgtcaagga ccgcaccttg agtgactatg
     9061 caggtaactg gcaatcagtt tatcctttcc ttgaagacgg cacgtttgac caagtctttg
     9121 actacaaggc taagttgact ggtaagatga cccaggctga gtacaaggct tactatacaa
     9181 aaggctatca gacagatgtg actaagatta acattactga taatactatg gaatttgttc
     9241 aaggtggaca aagcaagaaa tacacttaca agtatgtcgg taagaaagtt ttgacttaca
     9301 agaaaggcaa tcgtggcgtg cgtttcctct ttgaagccac agatgctgac gctggacaat
     9361 tcaagtatgt tcagtttagt gaccacaata tcgccccagt taaggcagaa catttccata
     9421 tcttttttgg aggtacaagc caagaaactc tatttgaaga aatggataat tggccaacct
     9481 actacccaga taacctatct ggccaagaaa tcgcccaaga aatgttggcg cattgatgag
     9541 aaatcgacta gttcataaga gctagtcggt tttttgatac tagatggcta acttcattat
     9601 agacctttca gaactaaggg aacctgaaaa aataaataaa actcttgaaa tatctgggct
     9661 tatatgctag aatgtagtta gtgtataagg ttcattagaa caccaaagcc cctaactagt
     9721 gccatagtta agggcttttt tgacgtatct tagcgattta acgggtgttt ataggctagt
     9781 cattcggtct acttcttacc atctagccat ttgcagacga aatacaagat aattccagct
     9841 ataacatctg ctataatagt aagagcaagc tctggtataa ggctcattag tttcacctcc
     9901 tctcacgaac ccataggaac gtaatcggta accgatgaca aaaatagtat accacactaa
     9961 atttagatca tcaacctcac ttaatacttg aactattgta gattatatgc taaaatgtag
    10021 ttaattgata aggttcatta gaacaccaaa gcccctaact atggccgtag ttaagggctt
    10081 ttttgacgta tcttagcgat ttaacgggtg tttataggct agtcagtcgg tctactcctt
    10141 actatctagc catctgcaga cgaaatacaa gataattcca gctataacat ccgctataat
    10201 agtaagagcg agctctatga taaggctcat tagtttcacc tcctctcacg aacccatagg
    10261 aacgtaatcg gtaaccgatg acaaaaatag tataccacaa tacatttaga tcatcaaggt
    10321 cacttaattc ttgaaatata gggtctaggc gacaaagtct ttaaaatcag aaaaacgcat
    10381 aatatcaggt gtgcaaaaac ttgatactat gcgttttatt gtgggaaggt ttactccatt
    10441 ttctcctgaa attgagtttt tgtccagcct ctgtttttag ggttgctgag aaaataatgt
    10501 catgtggtga atatttgtaa atcagtcagc agacagaacg aatactcttc gaaaatctct
    10561 tcacatcatg tcagcttcgt ctttccgtat atatgtgact gacttcatca gttctatcta
    10621 caacctcaaa acagtgtttc gagctgactt catcagtttt caaatctgta ctttgagcaa
    10681 gctgagacta gcttcctatt tgattttcat tgaatatcag aaacccattc tccatcaaat
    10741 aattcgactg cgtctaataa tttttgatct ggcacggtgt ctgaaataaa ggttgtgtat
    10801 ttggagaggg gattaatttt aaaaaatcca gtcttgtaaa atttagaact atcaatcagt
    10861 aagatggttt catgggcttt gtcaataata ttcttttttg aaatagcttg actgagagaa
    10921 gcttcataaa catattggtc atcaatacct cttgctgaac aaaatgctaa atcgatatta
    10981 aaatgatcta ataaagaatt ttccttatca tagttgacca cggaacagga ttgatgtttg
    11041 acctcgccag atgtgataaa gattttggag ctatctttaa cagtttcaga tagggtttgt
    11101 gcagtatgta aaccatttgt aaaaataatc aaattatcaa gttcagaaag atagggacag
    11161 agttcgtaga cagtcgtact ggaatccaga tagatacaca taccagaccg aataaagtct
    11221 ttagcgagac tagcgattag tcttttttgc ctagtacttt ctccttcacg tatttgatga
    11281 gaaagttcaa ttgtgttcat agaggacagg gtcacgtatc cgtgctttct tttgataaga
    11341 ccttgatttt ctaagaaaat taaatcacga cgtaaggtac ttgtgctgga gaaagtgatt
    11401 tctgccagct cttttacggc aattcttttt ttctttttaa taatttcaat caattcaagt
    11461 acacgttcat cttttatcat aagctcctcc taatttatca tttcaactat attatagcac
    11521 aaattggagg aatttgaatt atttttatga atattgggtt aacatttaaa cattattcaa
    11581 gtaagcgttc acatattgaa aaaataaaac gtggggatta taataaagtt aatcaaggac
    11641 gaagagagaa gaaaaatgga agcggtttta gcaatagatt taggtgcgac ttctggaaga
    11701 gcaatcgttg gttacctttc tgaaaataaa ctagtaatgg aagaaataaa tcgcttttct
    11761 aatctaccta ttagagtaaa agggcattta tcttgggata ttgactttct actagctaaa
    11821 attcttgaaa gtatccgctt ggctaatact agttacaaga ttttatctat cggtattgac
    11881 acatggggag ttgattttgg actgattgat aatgaaggta agctgttatt acaacctgtt
    11941 cattatcgtg atgaaagaac aaagggagtg ttaaaggaaa tatctgaaat gactgaatta
    12001 gaaaaactgt attcagagac aggaaatcag attatggaga taaatacctt gtttcaactc
    12061 tttaaggcac gtcaagaatc tcctgactct ttctataaga ccaataagat tcttttaatg
    12121 ccagatttgt ttaattatct cttgacaggt aagtttgcta cagaaaaaag cattgcttca
    12181 acaactcaat tatttgatcc taggagtcaa aattggaatc agaatatctt aaaactattt
    12241 gaattggatt caactttact tcctgaaatt gtttcagagg gaaatgtcct tggaaggata
    12301 aaagaggagt atggtttagg cgatattcct gttgtgaatg tttgtagtca tgatacagca
    12361 agcgcgattg tctcagtacc taagacagaa gatagtttgt ttatttcatc agggacttgg
    12421 tctttggttg gagtggagct ttcttcacct attcttagta ccgaatcctt cagttatgga
    12481 tttacaaatg aagtcggtaa agatggagtg attacatttc tgaagaattg tacagggttg
    12541 tggatcatag aggaactaag acgttcattt gaacgaagag ggaaagccta ttcttttgat
    12601 gatattagga caatggtgga gaaagaaaaa gaaaatcttc ctctgattga tactgaatca
    12661 actgaatttg caacagaatc tgatatgcac aagactttga cagaatatct agcttatcat
    12721 catgaaactg gagagtggac agatggacaa ctatttaaga tcgtttatga aagcctagct
    12781 gaaacgtata aggaaacaat agagttacta gaagaactaa ctcataaggt ttataagagg
    12841 atatatgtga ttggaggagg tgctagagcc agttacttta accaaatgat tgctgataga
    12901 actggtaaag aagttcttac aggttcaact gaggctacag ctgtggggaa tatttgtata
    12961 cagctattaa gtcaaggtaa aataaacgag gatacagggt taaaggatat tatgacaaat
    13021 attgctgata cacaatatta ttatcctcag ctatcataaa caaaaataaa aaaacggagg
    13081 ttttgtcatg aacaaaaaaa gtcttttgaa atgcgctgtt atcgggttag tagctacctt
    13141 tggtttagca gcttgtggaa catctaagga tgcaagcggt ggaagttctt ctggtaaaga
    13201 agtattagaa ttttatcatg ggtatcatca tagtgaagat gaatggcctg tagcgaagac
    13261 tatgcgagat ttatatgata aatttgcaga agaacataaa gatagtggtg tagaatttaa
    13321 accaactcct gtaaatggtg atttgaagga tataatgaat aacaaggttg ctagtggtga
    13381 atttcctgat gtgattgacc tagcaggtaa tgcagtatca ttagctgcga ttgaacaaaa
    13441 acttgtttta gatttgaaac catatatcga ttcaaataag cttgaaaaaa atgttggttt
    13501 gaactataaa caaaaccaaa aagatggaaa aatttataca gttcacgaac agttatttac
    13561 aatgggtctg tggtacaaca aggacatatt tgcaaaagca ggtgctaaga cacctgatca
    13621 gtggaatact tgggaagatt ttacacaagc aatggctgct gtgagaaaac aagatggagt
    13681 gtatgcattt ggtgctggag aaccatctat tcgcttgttt aatacagtct taggaaccac
    13741 agaaaatggg cgtaaattat tagacaaacc attaacaaaa gaaggaattg aatctaaaga
    13801 atttgcagat gctttgaaga tggtcatgaa agaaattcaa gctaatggtt ctaagaatgc
    13861 tggtggcgat gctaatgcgt attctaaaga cttccaagaa ggtaaatctg cagtcttctt
    13921 taatggtgtg tgggcttctg gtgagatgtc taagaaccca agtcttgcac ctggaatcta
    13981 tcctgcaggt gtagcaatta gttcttctgg aggtggtatc actatctcaa gtaagatgtc
    14041 tgaagctaaa caaaaactag ctcttgagtt tttgaaatat atgactagcg atgatgtaca
    14101 aaaagtaatt tttgaaaaag taggagcaaa tccttctaat gaaaatgtaa atgtaaaaga
    14161 actttcagaa aagagttcag aagctactac taaaatttta ggacaagcaa tcactcaagt
    14221 taagaatgca aaagcagttg ttccaactgt aagtgatgtt tggggtggcg atgtacacac
    14281 agctattatc aatgcattga cagaaagtgc tgctgaaaat gtagatgttg atcaaaaagt
    14341 aaaatctact caagatgtgt tgaaatcatt gattggttaa ttgttttagg gagtgaaaac
    14401 gaagctcagt tggcactgcc aactgagctt gattttactt atatagttga aaaaaggagt
    14461 ttgtttatgg cacaaaaaat tatgagccta caaaatagga agaatcaaaa aagacgattt
    14521 atctttttat ttcttttgcc aacgttgatt tgttt
//
LOCUS       DAVMHG010000042        14345 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMHG010000042 DAVMHG010000000
VERSION     DAVMHG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.042"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..412
                     /locus_tag="VYR08_001024"
     CDS             <1..412
                     /locus_tag="VYR08_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3466567.1"
                     /translation="LICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSSV
                     VFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATV
                     TKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            422..736
                     /locus_tag="VYR08_001025"
     CDS             422..736
                     /locus_tag="VYR08_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV3466568.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            767..1288
                     /locus_tag="VYR08_001026"
     CDS             767..1288
                     /locus_tag="VYR08_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466569.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKFINEQIRLINNLSN
                     WKRTHLVYNPDLV"
     gene            1352..2698
                     /gene="celB"
                     /locus_tag="VYR08_001027"
     CDS             1352..2698
                     /gene="celB"
                     /locus_tag="VYR08_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV3466570.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVF
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            3077..3253
                     /locus_tag="VYR08_001028"
     CDS             3077..3253
                     /locus_tag="VYR08_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466571.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            3655..5868
                     /locus_tag="VYR08_001029"
     CDS             3655..5868
                     /locus_tag="VYR08_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEV3466572.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNMSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(6090..6566)
                     /locus_tag="VYR08_001030"
     CDS             complement(6090..6566)
                     /locus_tag="VYR08_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV3466573.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            6789..9992
                     /locus_tag="VYR08_001031"
     CDS             6789..9992
                     /locus_tag="VYR08_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV3466574.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQKDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKHGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            10372..10748
                     /gene="tnpA"
                     /locus_tag="VYR08_001032"
                     /pseudo
     CDS             10372..10748
                     /gene="tnpA"
                     /locus_tag="VYR08_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(11135..11764)
                     /locus_tag="VYR08_001033"
     CDS             complement(11135..11764)
                     /locus_tag="VYR08_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV3466575.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNKKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAIKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(11774..12775)
                     /locus_tag="VYR08_001034"
     CDS             complement(11774..12775)
                     /locus_tag="VYR08_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV3466576.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(12806..13447)
                     /locus_tag="VYR08_001035"
     CDS             complement(12806..13447)
                     /locus_tag="VYR08_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV3466577.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(13466..14281)
                     /locus_tag="VYR08_001036"
     CDS             complement(13466..14281)
                     /locus_tag="VYR08_001036"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV3466578.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPSVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4529 a         2411 c         3018 g         4387 t
ORIGIN      
        1 tttaatatgt actgaatcag acaaggtaaa tttacttgaa aagatggttg agagtttatc
       61 tgttggtgaa tcaaatgagt ttgaacaatc tttactttat ggaattaaac agcgcgaaga
      121 attaagttca gttgtttttt ctgaaaaaat tgcagttccg catcctattc agccctttgg
      181 tacagaggga aaggtttcgg ttgcaatttg taaagattcg ttactctggg ataatcaaag
      241 ttcacatgtc cagctagtat ttcttctatc tccatcaata tatgggaacg aaggtttggc
      301 aactgtgact aaaaagatag tctctctgac agagaatgat gaattgcaaa atcagctaat
      361 atcttgcaat aactttgaag attttattaa catatttgag aagataaaat aggaggttca
      421 tatgaatcaa gaggaactgc aggttgcagc atttgaaatt atcctgcatt cgggaaatgc
      481 aaggtcagaa atacatgaag catttgcaaa aatgagagaa ggtagttttg atgatgcaga
      541 aagtaaatta aatcaatcaa atgagataat tcttgaagcg catcatgcac aaaccaaact
      601 cttgcaagaa tacgcaagtg gagtggaaat taaaattgaa attatcatgg tacatgctca
      661 agatcattta atgaccacta tgacattatt agaagtagca aaggaaatgt tagctcttta
      721 taagaaagtt aactaaatat gtgttgttat ataaggagga aatgatatgg ataaaaaaga
      781 acgacaaaag attgaacaac aacgtcgaga gatggctcta actaatactt tttttaatcg
      841 atatcttctt ctacgttatt caatagcact cttctttttt gggaatattt attggctttt
      901 gaatcagttt attagtccct cgcctattat tattttcccc attatgctca tagtattttc
      961 tattttagca actattgaac aatttaaact ttatggaaat aggaaagaaa agttgggaat
     1021 aacactaatg tttgttagaa tacaaatact tatttcaata ggattacttg ttttaacttg
     1081 gacaagttgg tttaagaatc tttttccgat ttttgaaaat aaccaagtag cacgtttatt
     1141 tgtttttgta gttcttctat taggtttagt tctgagtttg ttagacatta gaagaattaa
     1201 aaaatttata aacgaacaga taaggcttat caacaatttg tccaattgga aaagaactca
     1261 cttagtttat aatcctgatc tagtataatt tgtatttgaa actagtcttt agaaagtaaa
     1321 aattattatg ttttattaag gaggtctatt tatggatcaa caaaacgggt tgtttggttt
     1381 tcttgaaaac catgttatgg gaccaatggg caaacttgct cagtttaaag tagtgcgtgc
     1441 tatcacggct gcaggtatgg ctgctgtacc atttactatt gtaggatcaa tgtttttggt
     1501 attcagtatt ttgccacaag ctttctcatt ttggccaatt gtggcagata ttttctctgc
     1561 ttcatttgat aaattcacat cactttacat ggttgcaaac tatgcgacta tgggttctct
     1621 atctctttat ttcgttctat cacttgcata tgaattgaca aaaatttatg cagaggaaga
     1681 agaactcaat atgaatcctc ttaatggtgc cttgcttgcc ttgatggctt ttgtcatgac
     1741 agtaccgcaa atcatttttg atggtggaat gatgaagact gtgacaagtc taaaagaagg
     1801 tgcagtaatt gcagatggat gggcaatggg aaatggagtc gcacgttttg ggacaacagg
     1861 gatttttacc gcaatcatta tggcaattgt gactgttctt atttatcgta tgtgtgttaa
     1921 acataattgg gttattaaaa tgcctgaagc tgttccagaa ggagtttctc gtggatttac
     1981 cgctttggtt ccgggatttg tttttgcatt tgttgttatc tttatcaacg gtcttcttgt
     2041 agcaatggga acagatattt ttaaagtcat tgcaattcca tttggttttg tatccaatct
     2101 gactaattcg tggattggtt taatgattat ttatctattg actcaactac tttggattgt
     2161 aggtatccac ggtgcgaaca ttgtttttgc atttgttagt ccaattgctc ttgctaacat
     2221 ggctgaaaat gctgctggcg ggcacttcgc tgttgcaggt gaattttcta atatgtttgt
     2281 aattgcaggt ggttctggtg caactttagg actatgttta tatattgctt ttgcctctaa
     2341 atctgaacag cttaaagcaa taggacgagc atctgtagtt ccggccttat ttaatattaa
     2401 tgaaccatta atttttggat tacctattat ctataatcca gccttggcta taccatttat
     2461 tttagcacca atggttactg ctactattta ttacgtagcg aattctctaa actttattaa
     2521 gccaattatc gcacaggttc catggccaac tccagtaggg attggagctt tcttagggac
     2581 agcagatctt cgagctgtat tagttgctct agtatgtgca tttgcagcat tcctagtcta
     2641 tcttccattc atccgtgtat atgatcaaaa attggtgaaa gaagagcaag gtatctaata
     2701 agtaaaaaag tatactcgat gatagaatcc atatagttta aataatgagt gttaatatta
     2761 gcagtgaaga ggtcttttta agatattctt ctctgctttt tcatattttt tcgctatgta
     2821 gggtatgaag caaaatagaa agtagttcga atagttgata aacagggaac tgctgttatt
     2881 taaaaaaaca ataaagtaaa gtatgttatc gtagaatacg atacaatagt aaaaaagcaa
     2941 aaagaatgcg ttttatattt gggtttgagg cttataaaat aggataagcg tgctgaaaac
     3001 ttagatatga caaacaaaat aaaattttta aaagctacag agaaacatgg tataatgtat
     3061 ctaaaggagg tagcatatgt ccttagatat agataaagaa aagatgacaa tcatgggaat
     3121 agcttttgaa aaccgatccg tttttaagag tgtttggtat gctttaagta caaatatgat
     3181 tgaaggatgg cgacctacgg ttagtgatgt tgaaaaatta cgagatgaag ctcttgctct
     3241 ggggatgact taatgaagcg caactgttat gagtcttatg actatgttga tccggataat
     3301 ttatataccg atccgggttc ttcagttcta agaaataagc aggaaggaaa aaagcacgcg
     3361 agcttgaata tcgtatgatt gctagtaaga gtttgaaatt atttatcaat cctatcttag
     3421 ttaatagttt aaaagtagtt tgtttcacaa ctgcataagc ttagaaaagt tgaaaagtga
     3481 taactcttta ataaactctt ggtttgttaa aagatatttt gtccactaca tttttttcaa
     3541 ttgactattg tttgtcatag atataacaga tagaacattg cattcagaat aatgaaaaac
     3601 gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa caaattgaaa
     3661 atttttaagg gagagtttta tcgaatctct gtattaacag acaagctagt aaggttagaa
     3721 tactctcaaa ctggaagttt tgaggataga acgacacaac ttatctataa tagagatttt
     3781 ggccaaattt cgttagatta tatcgagaca tcaaacgtac tagatattat gacggactat
     3841 tttcatctgc actttaataa aggagaattt aacgccgaaa atttatttat agaattaaaa
     3901 ggaaattttg ccgtatatgg tagtcgctgg tattttggtg aatctattga aacgttaaaa
     3961 ggaacagctc ggactctgga taaggcagat ggagcaatct cgttagaaga tggaattatt
     4021 agccgaaatg gtatagcctt attggatgat tctcaaggat ttatttggga tgaacaatct
     4081 ggttatattg agagagaaaa tcaaattgac ctgtatttct ttgcctatgg gcatgattat
     4141 agaggagcaa tcagagactt ttaccatttg actggttcaa cacccttgtt gccaagatat
     4201 gctttaggca attggtggag tagatattgg ccttatacgt cggatgaata cttggattta
     4261 atagacagat ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga tatggattgg
     4321 catataactg acattccagc tcgttttgga agtggctgga caggatatag ttggaataga
     4381 aacttaatac caaatccaga acagttattg caacaacttc atgatagaaa gttaaaactc
     4441 tccttaaatg tccatcctgc tgatgggata cgggcttatg aagaagctta tcctcaaatc
     4501 gcaaaacgat tggggttaaa tgtagaacta gaagaacctg ctatttttga tttttttaat
     4561 ccctctttta gggaagccta ctttaaagat gttcattatg agctagaaaa gcagggagta
     4621 gatttttggt ggattgactg gcaacagggg acacaaggca tgctagatcc actttggctt
     4681 ttaaaccatt atcactatca ggatagttgt aaaaatgcag aaggtggttt gattttatca
     4741 agatatgcag gtcctggtag tcaccgctac cctgttggtt tttcagggga tactattatt
     4801 agttggaatt ccttaagatt tcaaccctat tttacagcga cagcatctaa tatcggttat
     4861 agttggtgga gtcatgatat cggtggacat atgttggggg attatgacga agagctacaa
     4921 actagatggc tacagtttgg tgtttttagt ccgattactc gattacatag ttctagaagt
     4981 ccttttaata gtaaagaacc ttggttcttt tcagaaacaa catctaagat tatgaagaaa
     5041 taccttcgtt tgagacatca gatgattccc tatctatata ccatgaatgt acagacacat
     5101 gaggaaggtg ccccattaat cagtccaatc tattatttct atccagagaa tgatgagagc
     5161 tataatgttc caaaccaata cttttttgga acagaactga tggtggctcc cattgtagaa
     5221 aagatggatt tgacattcca atctgcaaaa gtagatgtat ggttccctga aggtgaatgg
     5281 tatgacttct tttcagagaa aaaatacaca ggtggtgtga agttaagtgt ttatagggac
     5341 atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag
     5401 ataggtatgg gtgttgattt acctgaggtt gtagattggt atgtattccc agggaaacaa
     5461 catttttttg aaatgcttga agatcaaaat ggtcaaagat ataaaacaag attatcaatc
     5521 gattgggaaa tgggaatggt agagttagca ttacaaggag attctagtat cgttccaagc
     5581 aatagaaaac atagaattca ttttaaagga acgaatatgt ctataattga attgccaaat
     5641 aagaatgata cagctaaatt tgaatggaaa gataataaaa ggacatcttt aaatgacgaa
     5701 gtttttagac tactaaagac agcttctctt ccatatgaat taaaagatag attgttaaat
     5761 caattcatca atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca tcaggataag
     5821 gaattgagag ggcgtttgtt ggaaatgata tttactagcc aaaactaatt gaaataatta
     5881 gaaagtcatt tgtttagtgt atatgaagat tctgtatggt attcgttatc actggtcaca
     5941 ttgaaaatgt gaaataagca cctacaacat agactttaca acacttcatg tttcatatca
     6001 tggggtgttt ttctatttaa gtcaaagccc atcaaaaaat ctcactattc atcaggttaa
     6061 aggaaaccta atcatagtga gattgtgaat tatagtagag tttgtatcgc ctcttcaatt
     6121 tgttttgggt ctgtttttga agaaaagcgt tcaaagactt gcccatcacg accaatgaga
     6181 aacttagcga aattccattc gattcgtttt cctagtgggc cagatttatg gtcttttaac
     6241 cagacataga gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag
     6301 gtggtttgaa aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc gggtgcttgt
     6361 cccataaact gattgcaagg gaaatccaat atttcgaagc cctgatcttg atagcgttca
     6421 tagagttctt gaagtccctg gtactggggc gttaaaccac atccagtagc agtgttgaca
     6481 atcaagagaa ctttgccacg atagctatcc aagggagttg cttggttgtt ttggttcaaa
     6541 acggaaaaat cataaagtga agtcatgagg gcctcttttc tttttctaca ttgtaacagt
     6601 ttttactttt ttcgtcgagt cggatggaag atattgttga agaataagcc ctcaatatga
     6661 tagaaaccgt ttacaaataa ctttaaaaat ggtataattt aattaaaccg gtttacttag
     6721 ttgctataaa tctctaattt gagatttagt tcctattgat tttacaatat gtttattgga
     6781 gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca cttgttttat
     6841 caggattttt attaaaccat tatatgacag ttggagcgga agaaacgact acgaatacca
     6901 ttcagcaaag ccagaaggaa gttcagtatc agcaaaagga tacaaaaaat ttagttgaaa
     6961 atggtgattt tggtcagacg gaggacggaa gcagtccgtg gacaggaagc aaagctcagg
     7021 ggtggtcagc ttgggtagac cagaagaata gttctgcaga tgcctcaact cgagtcattg
     7081 aggctaagga tggggctatc actatctcaa gccctgagaa attaagggca gcggttcacc
     7141 gtatggttcc tattgaagct aagaaaaagt ataaactgcg tttcaagatt aaaacagatc
     7201 ataaagtcgg gattgccaaa gttcgtatca ttgaggaaag tggtaaggac aagcgattgt
     7261 ggaattctgc aacgacgtca ggaacaaagg actggcagac cattgaagca gactatagcc
     7321 cgactttaga tgttgataaa atcaagctgg agttattcta tgaaacagga actgggactg
     7381 tttcctttaa ggatattgag ctggtagagg tagcagacca gctttctgag gattctcaaa
     7441 cagataaaca gcttgaggaa aagattgatt taccaattgg aaaaaaacat gttttttctc
     7501 ttgcggacta tacttataag gtagaaaatc ctgacgttgc ttcagtcaaa aatggaattt
     7561 tagaacctct taaggaaggg acaaccaatg tcattgtcag taaagatggc aaggaagtga
     7621 aaaagattcc tttgaagatt ctagcctctg ttaaggatgc atacacagac cgtttggatg
     7681 actggaatgg catcattgct gggaatcaat actatgattc taaaaatgaa cagatggcca
     7741 aattaaacca ggaattggaa ggaaaggtag ctgatagcct atctagtatt tcaagtcagg
     7801 cggaccgcac ctatttgtgg gaaaaatttt caaattataa aatgtctgca aatctgactg
     7861 ccacttatcg gaaattggag gagatggcca agcaagtgac caatccttct tctcgttatt
     7921 atcaagatga aactgtcgtt cgaacagtca gggattccat ggaatggatg cataaacatg
     7981 tctacaatag tgaaaagagc attgttggga actggtggga ttatgaaatc ggtacacctc
     8041 gtgccatcaa caataccttg tctctgatga aagaatactt ctctgatgag gaaattaaaa
     8101 aatatacaga tgtgattgaa aaatttgtac cagatcccga acatttccga aagacgactg
     8161 ataacccatt caaggctcta ggtggaaact tagttgatat gggaagggta aaagtaatag
     8221 ctggtttact gcgtaaggat gatcaagaaa tttcttctac cattcgctcg attgagcaag
     8281 tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga tggatcctat atcgaccata
     8341 ccaatgttgc ctatacgggt gcttatggga atgttttgat tgatggcctg tctcaactgt
     8401 tgccagtcat tcaaaagacc aagaatccaa tcgataaaga taaaatgcaa accatgtacc
     8461 actggattga taaatcgttt gctcctttgc tggtgaatgg agagctgatg gatatgagtc
     8521 gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt ggccgcagta gaagtactaa
     8581 gagggattca ccgaatagcg gatatgtctg aaggagaaac caaacaacgt ttgcagagtc
     8641 ttgtgaagac cattgttcaa tcggatagtt attatgatgt ctttaagaat ttgaagactt
     8701 ataaggatat cagtttgatg caatccttgt taagtgatgc aggagtcgca agtgttccaa
     8761 gaacaagtta cctatctgcc tttaacaaga tggataaaac agccatgtac aatgcagaga
     8821 aagggtttgg atttggcttg tcactctttt ccagtcgtac cttgaattac gaacacatga
     8881 acaaggaaaa taaacatggt tggtatacga gtgatgggat gttctatctt tacaatggcg
     8941 atttgagtca ctatagcgat ggctactggc caacagttaa tccatataag atgcctggta
     9001 caacagagac ggatgctaag agagcggata gcgatacagg taaagttcta ccgtctgctt
     9061 tcgttggaac gagcaaacta gatgatgcca atgcgacagc aaccatggat ttcaccaact
     9121 ggaatcaaac attgactgct cataagagct ggtttatgct gaaggataag atcgcctttt
     9181 taggaagcaa tatccaaaac acttcaacag atactgctgc aactacaatt gaccagagaa
     9241 aactggaatc aagtaatcca tataaagtct atgtcaatga taaagaagcc tcccttacag
     9301 aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt agaatcatcc gattcgaaaa
     9361 agaatattgg ttactttttc tttaagaaga gttcaatcag tatgagtaag gctttgcaaa
     9421 agggagcctg gaaggatatc aatgaaggac agtcagacaa ggaagttgaa aatgaatttc
     9481 ttacgattag tcaggctcat aagcaaaatg gagattctta tggctatatg ctcatcccta
     9541 acgtgggtcg tgccaccttc aatcaaatga taaaagagtt agaaagtagc ctcatcgaaa
     9601 ataacgaaac ccttcagtct gtttatgatg ccaaacaagg agtttggggc attgtgaaat
     9661 atgatgattc tgtctctact atttccaacc aattccaagt tttgaaacgt ggagtctata
     9721 ccattcgaaa agaaggggat gaatataaga ttgcctacta taatcctgaa acccaggaat
     9781 cagctccaga tcaggaagtc tttaaaaagc tagagcaagc agctcagcca caagtacaga
     9841 attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca ttcggatcaa aagaatctcc
     9901 ctcagacagg agaaggtcag tcaatcttgg caagtctagg gttcttgcta cttggggcgt
     9961 tttatttatt ccgtagagga aagaacaact aatttgttca tcatctagat gaattacatg
    10021 aaactgtgga aaatagaaaa aatccgaaga aggccaaata tcttcggatt tttcttttaa
    10081 aaaaggataa gttgatactt atccttttta cttcattatg tgactatagc gttttgaaac
    10141 tagaatagga catcataact gctaaaagat ttctataaat tcatttgatt ttcctaatca
    10201 atttattcgt atcctatttc actctactat attagtccgt ctcgcacctc acggagcgag
    10261 acaaaacaac cacccgctat gcgggtgcgc gtcgaaggtt ataccaaaaa aactccaaac
    10321 gcgatacaat aaaggtgttc aatccacttg taaagcgaaa ggagaaaaaa tatggcacaa
    10381 aaggctcata gtttatctca cacaaagtgg aggtgttcta tcacattgtg ttcaccccta
    10441 agtatagacg aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc
    10501 gcttgtgtag ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac
    10561 acatgttagt cagtattcca ctaaggctaa gtgtttcgag tttcatgggt tatttaaaag
    10621 gaaaaagtgc actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc
    10681 ggcatttctg ggtggaaggt tattatgtaa gtacagtaga gcttaataaa gccacaatta
    10741 agaaatatag tcaagattaa agaaattatc cagtggatga tttcttcatg agtatgaaaa
    10801 tttgagaacg agtaaagcat gatatatagt aaaatgaaat aagaacagaa caaatcgatc
    10861 aggacagtca aatcgatttt taacaatgtt ttagaagtag aggtgtacta ttccagtttc
    10921 aatctactat agcactagat aaattgagtg taaaagaata tgaggatccc tttagggata
    10981 gtggtaagta ataccaaagt ctcttaaaga ggcaagtgac gagtcaagag caataaggct
    11041 tgaacaacgt gaaagccagc gtctttaggc gctggctggt aatttgggct tatagctctg
    11101 ggataaacca cccgtttgac gggtggttat gattttatct gagtgtaaca tactgttggg
    11161 caatctcgct gatgcggtca aagttgcctt gggaagcgag tttattgagt tcgccaccaa
    11221 ttccaacggc gtctgcacca gcagcgaacc attgagggat gttgtttaga ccgactcctc
    11281 cggttaccat tacggaaact tgtgggatcg gtgccttgat tgcagagata tatgctggac
    11341 tgagagcact acctgggaag agtttgatga tttcactacc ggcttcaagt gcagtcgtga
    11401 tctctgtgag ggtaatacag cctggaatgt acggtgtgct gtagagattg cacattttcg
    11461 cagtttcagc atggaaagat ggagaaacaa cgtaatttgc tccggctaga atggcatctc
    11521 tagcagttac ggcatcaagc acagtacctg caccgataca aacactctga tcgtcctgat
    11581 acaagtctac aagttctttg atgatttgtc ctgcatactg attggtatag gcgatttcaa
    11641 tagctttgat accgcccttg atacaagcaa tcgaggcttg tagtccttct tcctttttat
    11701 ttcctcgaat gacagcgaca attttctgtt tttttagttc aataatcgta tctgatttgg
    11761 tcatgtaatt ctcctaacga atgatatctt gtgcatttgc cagtaaattt tcaatactag
    11821 ttgcggaagt ggagagatgg tctcctggaa gagtgcattt gagagttgcg ctcgcaactg
    11881 caaagtcaat ggtagttttt agggaggaat gatggagtag ttggtaaagg gcaccagata
    11941 taaaggcatc cccgctacca attcgttgat agactgcagt ttttagttgg acagactctt
    12001 caaatagttc ttctagagca taggcttgat agacattttt gtcttgctca tcactagagc
    12061 ggagggtatg gaaaatggcc ttgaaaccat aggcttcttt taaaagtcgc atgcgatttt
    12121 ccacctcttc taggctagca ctgtctcttg gaaacatctc tagattttgg tcatcaatca
    12181 tgagaggatc aataccgaag caatagtcag taaaacgtgc aaacttagaa aattcatact
    12241 tggcttctag gactgaaatc atctttgttc tcagattgag atccattgaa acgacaattc
    12301 ctcggcgctt ggcttcttcc aagagtagga gaaggatcgc acggacctct tgaccgatag
    12361 ctatggtgat tccactaaaa tgaaaatggc taatcccctg aaagagagaa tccatatcta
    12421 gcatgtttgg ccgaatctgg ctgatactcg tatgcttacg atcgtagaaa acttcacttt
    12481 gacgacaacc aaagccgttc tccaaatagt agaggccgat tcgatcgcca agccgacaga
    12541 ttgaactggt atcgatttgg tgctgtttca agaatgtgag aaaacgatct ccaatctcgt
    12601 tggcagggag tgcggtaaag actttcgttg agatacccag ggcttgcaaa ttacaagcga
    12661 tgttaatttc tgatccgcca aaataagtcg aactggcaac atgatcgccg atactggtgg
    12721 catctaatgg tgagattcga attaatggtt cgccaaacag taatattttc gtcatccatt
    12781 agttcctttc tggtttagaa tagctttact tggctaatac ttctttcaaa taagcagcga
    12841 cttcatcatc ttggcagttt tcaaagaagt attcttggaa gtatttgcca gagatggtgt
    12901 ctttgaggaa gtcttggtcg attgttttaa ggatggtcat caaatcattg tgggtgattt
    12961 gtttcacctc gtttaagata cgagcgttgc gttgttcagg gattacacgt tctcttgggt
    13021 agccaccgcc catttcttca gcaaataagc gttcaaacat caatttgagg gtcagttctg
    13081 ccccccagcc aaatcctttg gcataaggga tagacaaggc gttaccacca ttgatttgag
    13141 aataaaggta agcgtcagtt gggtccactg ctagaccaca gacaacacca gggaagctgt
    13201 ttaaagcaag catagcccct acacccgtac cacagccggt aacaacaaag tcaactgcct
    13261 ttgtatttaa aaggatggca gccattagtc cgttctgcac ataagttaat tgactttctc
    13321 cttcttctcc acgcatacca tagttaaata attggtagcc ttttttatct gtcgcttctt
    13381 ttagactatc gtaaataatg tgattcttgc tagcttgact attttcattg attaatgcga
    13441 ttttcataag atcttctttc tatttttact caggttgttt tccgatgtag gctaagatac
    13501 cgccatctac atataggatg tggccattga caaaattgct ggcatcacta gcgagaaaga
    13561 cagaagggcc catcaaatct tcaggatttc cccaacgtgc agcaggtgtt tttgcaatga
    13621 tgaactggtc aaatgggtgg cgagaaccat cttcttgcaa ctcacgaaga ggtgctgttt
    13681 gaggagtggc aatataaccc ggtccaattc cgttacattg gatattggct ccaccgtatt
    13741 cagacgcaat gttgcgggtc aacattttca agcccccttt agcagcagca taagcgctaa
    13801 ctgtttcacg tcccagttcg ctcatcatcg aacaaatatt gataattttt ccatgccctt
    13861 tctttatcat agaaggaata actgcctttg aaacgataaa tggtgcgttt aagtcaatat
    13921 cgatgacctt acggaaatca gcggcactca tttcgcacat tggaactcgg cggataatac
    13981 cagcgttatt aacgaggatg tcaatgacac caacctcttg ttcgatttgc ttgaccatgg
    14041 cttggatacc gtcctcgtct gtcacgtcac agacatatcc atgggcttgg atgccaactt
    14101 cacgataagc ttcaatccct ttattgacca gatcttgatt gatatcgtta aagacaatag
    14161 tagcgccggc ttcagcgtag gatttggcaa tagcaaatcc aattccataa gaagcgccgg
    14221 tgatgagagc aatttttcct tttaaagaaa actgctcaat tgagaatgat gtatttgtca
    14281 tttgatactt cctttcaaat ttgttaagac ttctctataa ccaaattata ttctagtaaa
    14341 ccggt
//
LOCUS       DAVMHG010000043        14316 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMHG010000043 DAVMHG010000000
VERSION     DAVMHG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.043"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(135..593)
                     /locus_tag="VYR08_001037"
     CDS             complement(135..593)
                     /locus_tag="VYR08_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV3466579.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(590..1030)
                     /locus_tag="VYR08_001038"
     CDS             complement(590..1030)
                     /locus_tag="VYR08_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466580.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1386..3335
                     /gene="mutL"
                     /locus_tag="VYR08_001039"
     CDS             1386..3335
                     /gene="mutL"
                     /locus_tag="VYR08_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV3466581.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3660..4127)
                     /locus_tag="VYR08_001040"
     CDS             complement(3660..4127)
                     /locus_tag="VYR08_001040"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV3466582.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4128..5333)
                     /locus_tag="VYR08_001041"
     CDS             complement(4128..5333)
                     /locus_tag="VYR08_001041"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV3466583.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRIHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5353..5988)
                     /gene="ribE"
                     /locus_tag="VYR08_001042"
     CDS             complement(5353..5988)
                     /gene="ribE"
                     /locus_tag="VYR08_001042"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV3466584.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5973..7073)
                     /gene="ribD"
                     /locus_tag="VYR08_001043"
     CDS             complement(5973..7073)
                     /gene="ribD"
                     /locus_tag="VYR08_001043"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV3466585.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7207..7321)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7478..8071
                     /gene="ruvA"
                     /locus_tag="VYR08_001044"
     CDS             7478..8071
                     /gene="ruvA"
                     /locus_tag="VYR08_001044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV3466586.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8081..8644
                     /locus_tag="VYR08_001045"
     CDS             8081..8644
                     /locus_tag="VYR08_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV3466587.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     VFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8641..9318
                     /locus_tag="VYR08_001046"
     CDS             8641..9318
                     /locus_tag="VYR08_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3466588.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9473..10504)
                     /locus_tag="VYR08_001047"
     CDS             complement(9473..10504)
                     /locus_tag="VYR08_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV3466589.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10759..11442)
                     /locus_tag="VYR08_001048"
     CDS             complement(10759..11442)
                     /locus_tag="VYR08_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV3466590.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11454..12398)
                     /locus_tag="VYR08_001049"
     CDS             complement(11454..12398)
                     /locus_tag="VYR08_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3466591.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(12560..13726)
                     /locus_tag="VYR08_001050"
     CDS             complement(12560..13726)
                     /locus_tag="VYR08_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV3466592.1"
                     /translation="MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKELK
                     QKANQEKIAFQKAGSTRQKASTIKNYQELANLWLESYKNTVKPNTQGNVRKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKGAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRQKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHVTKTLNRDIEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRESVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
BASE COUNT         4060 a         2750 c         2874 g         4632 t
ORIGIN      
        1 aaaacattgt tagaaatcta tttgactgtc ctgattgatt tatcctgttc ttatttcatt
       61 tcactatagt aaggtgcagc aggataaagc aaaaccgaga agtttctttc tcggttttgc
      121 ttattatgag gttcctattt tctatgctcc aagagttgat tgatgtgatc tactttttta
      181 gcctctcttt tatagagaat agcccagatg atgagataga caatcgcaaa ctcgataatg
      241 agctggaggt agaaaatcca gtggaagggg aaccaaccag caagagttgc tagtgggaca
      301 aagccagcca gcataaggaa gaaatgagtc agagtggcac gaagcatgct ccagtcacgg
      361 ctgaataagc ggttgccaaa gttgaagagc ataccgatgg ctgcccagat aagtgtgcag
      421 tagagcaaga ccagggcacc gtgaacctga tgctgatcca tcacttggcc gatgagagag
      481 tagggattta gtggtgcgta ggtatttggt gcataaatga gtgaaaagag gatggagagg
      541 atgaggccga taagaacacc ggcagctgca tcgtgaaata cttgtttttt catagttcta
      601 atttctcctt gatggttttt agataacggc gtgaagagta ggtgaagctt tcgtttttca
      661 agaaaatttc taccagaccg tttggcgtga gcttgaggtg agagatggaa tcgatattga
      721 tgatttctga ttgggaaatt tggataaaat tggttggcaa gagtttaaga acctgataga
      781 gtcgcaaatc aatgctgtag gtctgactcg cggtttctgc tagaaccttc cgattctcga
      841 tatagaagcg ctgaatcttg ccaatctcaa ctagatagac ctgatcatcg atttttcctt
      901 tgattttttc tctttggtcc agattttctg cgaactcgat gactttctgg actttttcgg
      961 tttcttgagg tgcttggaca atcagctttt cctcctcgta agtctcacta atctgtagtt
     1021 ctactttcat aaatttctct ccttgttttt catacaaaat taatctcact tcttgtacac
     1081 ctttattaat actcttcaaa aatctcttca aaccacgtca gcgtcgtctt gccgtaggat
     1141 agttgttgac tttgtcagtt ttatctacaa cctcaaaaca gtgttttgag ctgtctgcgt
     1201 ctagctttct agtttgctct ttgattttta ttgagtatac cactatttta ctccctctgg
     1261 caagggactt tgtctatgtg gagggattgg gctcctatgt ggtggagttt ttctgttctt
     1321 tctgaaatat ggtataatag cactaattaa tttctaggaa aatagataca gaaaggggct
     1381 gaaagatgtc tcatattatt gaattgccag agatgctggc aaaccaaatc gcagctgggg
     1441 aggtcattga acgtcctgcc agtgtggtta aagagttggt agaaaatgcc attgacgcgg
     1501 gctctagtca gattatcatt gagattgagg aagctggtct caagaaggtt caaatcacgg
     1561 ataacggtca tggaattgcc cacgatgagg tggagttggc cctgcgtcgc catgcgacca
     1621 gtaagataaa aaatcaagca gatctctttc ggattcggac gcttggtttt cgtggtgaag
     1681 ccttgccttc tattgcgtct gttagtgtct tgactctgtt aacggcggtg gatggtgcta
     1741 gtcatggaac caagttagtc gcgcgtgggg gtgaagttga ggaagtcatc ccagcgacta
     1801 gtcctgtggg aaccaaggtt tgtgtggagg atctcttttt caacacgcct gcccgtctca
     1861 agtatatgaa gagccagcaa gcggagttgt ctcatatcat tgatattgtc aaccgtctgg
     1921 gcttggccca tcctgagatt tcttttagct tgattagtga tggcaaggaa atgacgcgga
     1981 cagcagggac tggtcaattg cgccaagcaa tcgcagggat ttacggtttg gtcagtgcca
     2041 agaagatgat tgaaattgag aactctgacc tagatttcga aatttcaggt tttgtgtctt
     2101 tacctgagtt gactcgggct aaccgcaatt atatcagcct cttcatcaat ggccgttata
     2161 tcaagaactt cctgctcaat cgtgctattt tggatggttt cggaagcaag cttatggttg
     2221 gacgttttcc actggctgtc attcacatcc atatcgaccc ttatctagcg gatgtcaatg
     2281 tgcatccaac taagcaagag gtgcggattt ccaaggaaaa agaactgatg accctggttt
     2341 cagaagctat tgcaaatagt ctcaaggaac aaaccttgat tccagatgcc ttggaaaatc
     2401 ttgccaaatc gaccgtgcgc aatcgtgaga aggtggagca aactattctc ccactcaaag
     2461 aaaatacgct ctactatgag aaaactgagc cgtcaagacc tagtcaaact gaagtagctg
     2521 attatcaggt agaattgact gatgaagggc aggatttgac cctgtttgcc aaggaaacct
     2581 tggaccgatt gaccaagcca gcaaaactgc attttgcaga gagaaagcct gctaactacg
     2641 accagctaga ccatccagag ttagatcttg ctagcatcga taaggcttat gacaaactgg
     2701 agcgagaaga agcatccagc ttcccagagt tggagttttt cggacaaatg cacgggactt
     2761 atctctttgc ccaagggcga gatggacttt acatcataga tcagcacgct gctcaggaac
     2821 gggtcaagta cgaggagtac cgtgaaagca ttggcaatgt tgaccagagt cagcagcaac
     2881 tcctagtgcc ctatatcttt gaatttcctg cggatgatgc cctgcgtctc aaggaaagaa
     2941 tgcctctctt agaggaagtg ggcgtctttc tagcagagta cggagaaaat caatttattc
     3001 tacgtgaaca tcctatttgg atggcagaag aagagattga atcaggcatc tatgagatgt
     3061 gcgacatgct ccttttgacc aaggaagttt ctatcaagaa ataccgagca gagctggcta
     3121 tcatgatgtc ttgcaagcga tctatcaagg ccaatcatcg tattgatgat cattcagcta
     3181 gacaactcct ctatcagctt tctcaatgtg acaatcccta taactgtcct cacggacgtc
     3241 ctgttttggt gcattttacc aagtcggata tggaaaagat gttccgacgt attcaggaaa
     3301 atcacaccag tctccgtgag ttggggaaat attaaaagta taaaaaagtc tgggaaaaat
     3361 tttcaaaatc agaaaaacgc ataaaatcag gtgttcaaaa accttgattt tatgcgtttt
     3421 atcatagaaa tatttacttc attttttcct aattcttttc gaaactcttt ttaaacgacg
     3481 tcagttttat cagtaatctc aaaacagtgt tttgagctaa ttttgccagt tttgtctgta
     3541 acatcgaagt tgtgtttgac cactctgcga ctggtttcct agtttgctct atgattttca
     3601 cagagtatta aattgcgatt ttgccaagtt tctttattcg tctaaaagta gagtctgttc
     3661 tatgcgtcta atgtacgaat taggttgacc atttcaatag ctccttgtgc acactcagaa
     3721 cccttatttc ctgctttagt accagctcgt tctatggctt gttcaattgt atctgtcgtt
     3781 agcacaccaa acataacagg aatttcgcta tttaaactga tttgggcgat tcccttagat
     3841 acctcgctac atacataatc ataatgactt gtattccctc taatgacagc tcccaagcag
     3901 ataattgcat catatttttt actttttgcc atttttgatg caatcagtga tatttcaaaa
     3961 gctcctggaa cccaggctac ctctatatct ttctcgttta cattctctct tttgagatta
     4021 tctagtgctc cagataataa ttttgaagtt ataaattcat taaatctcgc tacaacaata
     4081 cctattttaa tattgtttgc tactaaatta ccttcataag tgttcattta tttttcctcc
     4141 atatttaaaa tgtgacccat tcgatttttc tttgtttcta aataaaaact atcgtaagga
     4201 ttggcttcta tttcgattga tattctactg gaaatagtaa ttccatattt ttctaactgt
     4261 tcaaccttgt caggattatt tgtcagtaaa tgaagtgact gaagtcccag atctttaagc
     4321 atttgtgctc caatatgata ttctcttaaa tcaccttcaa agcctaatgc aagattggca
     4381 tcaagcgtat ccatgccttg atcttgtaaa tgataggctt ttaatttatt gataagtcca
     4441 attcctcgtc cctcctgtcg caagtaaagt aagacacccg aaccattctc aacaatcatt
     4501 ttcatagctt tatcgaattg ctgtccacaa tcgcaacgta aagagcctaa aacatcccct
     4561 gttaaacatt cggagtggat ccgacataat acattggctt catcctctat gtttcccata
     4621 ataagagcaa gatgatgttc cccatttagt ttatctatat agctaattgc tttgaaatta
     4681 ccgtatctag taggcatatt gacagttgaa actcgttcta ccagctgatc atatactttt
     4741 ctatattctt gtaattcttt gatggtaatt agtggaatgt tgtgtttctt cgagaactga
     4801 attaaatcat ctgttctcat cattttgcca tcatgattca ttatttcaca acataggcca
     4861 cactctttta gtccagctaa ttttaataaa tcaacagttg cttctgtgtg tccacttctt
     4921 tctagaacac cacctttttt tgcaattaaa ggaaacatgt gtcctggcct gcgaaaatca
     4981 gagggtgtta tatcttcagc tacacacata cgtgcggtca gtcctctttc ctcggcagaa
     5041 atacctgtgg tcgtttcttt ataatcaatt gaaactgtaa aagcagtctt atgattatct
     5101 gtattgtttt caaccatagg tgaaagcatt aattgattag ctaaactttc gctcataggc
     5161 atacaaatta atcctttggc ataagtagcc ataaaattaa cattttctgt tgtagctgct
     5221 tgtgcagaac aaattaagtc tccttcattt tctctatcct tgtcgtctat aacaagaaca
     5281 agtcgtccct tctgcaatgc ttctaatgct tcttgtattt ttcgatattc cattgactga
     5341 ttatcctttc tgctaaaatc cattttgata taatagttcc ttagatattt ctgattttgg
     5401 agagttatcc atcagttttt gcacatattt acctaagata tcattttcaa gatttactgt
     5461 actcccgact tgtttactct taagaatggt ttgttccaag gtatgaggga taacagatac
     5521 tgaaaagttt actttggaga ctttagcgac agtcagacta atgccgtcaa ttgtaataga
     5581 tcctttttca actattaaat ctaaaatttc tttttgtgtg ttgatttgat accatacagc
     5641 attatcatct ttttttattg acgagatttt tcctgtacca tcaatgtgtc ctgtaacgac
     5701 gtgaccccca agtcgaccgt tgacagataa ggctctttct agattcacct cacttccatg
     5761 ttttaataga gtaagagctg ttcgactcca tgtttcattc attacatcaa ctgtaaagga
     5821 ttgatgattg aaatgagtaa ctgtaagaca gataccattt actgctatac tatcgcctaa
     5881 atggatatcc gttaatattt ttgaggcttt aattgatagt ttacaattac gagagtcttt
     5941 ctgtattctt tcaacttttc cgatttcttc aattattcct gtgaacatgg ataaatcact
     6001 tcactttcta tgagatagtc atttcctatt tgagaaaatg cataaggttt caatctaata
     6061 gcgtcatttg gcaaagaaat accttcacct ccgacaggaa acttggcact acctccaaaa
     6121 atttttggtg caatatatat tttcagctca tcaacaattt gttgttccaa agcactccaa
     6181 ttcattagac tgcccccttc tagaactagg ctatcaatct gcatgtttcc tagatgttgc
     6241 attaaactcg ataagtctat atgattgcct tttttcttta tggaaagtat ttcacagcca
     6301 tgattttgat atagcttcat tttatttttg tcttcagagg aagtggcaat gtaagtttta
     6361 atatcatttg ctgtttttac gattttagag gtaagaggag ttcgtaaatg tgtatcgcat
     6421 atgatacgga taggattttt cccttcctcc aatctacatg tcagcaaagg atcgtcttga
     6481 ataacagtat tgactcccac cataattgca ctaacatggt gtcgtaactg atgcacatgc
     6541 tttcttgctt cttcttcagt aatccatttg gattgatttg ttttagtggc tatttttcca
     6601 tccattgaca ttgcatattt cataaaaaca tagggtacat gctgggtaat atactttcta
     6661 aaacttttta ttaagttaag acactcattt tctaaaattc caacagtaac ttgaagatta
     6721 ttttcctcaa gtatctttac tccttttcca gatacaatag gattacagtc taggcttcca
     6781 atgactactc ttgtaatacc actatcgatt atagcatcta tacagggagg tgttttcccg
     6841 aagtgacaac agggttcaag tgttacataa agcgtcgctc cgacagggga ttctctacag
     6901 tttttaagag catttctctc agcatgtggg ccaccaaaaa actcatgata accttgtccg
     6961 ataatgtgat tatcttttac aataactgcg ccgaccatag gattgggatt gacgtaacca
     7021 gccccttttt gtgccagttt tattgctaat ttcatatatt ttgaatcgct catctcacta
     7081 cctccaaaaa aatatgcctt gaatagggga ctactcaagg catacaaaag aaaacttatg
     7141 cgattaacaa aaatgctctg aaatgacaag taatcatttc agagcacgca aaaagcacaa
     7201 atatactttt atcttctttc atccagacta tactgtcggc tttggaattt caccaaatca
     7261 tgcctttcgg ctcgtgggct ataccaccgg tagggaattt caccctgccc tgaagatagt
     7321 tattcaatta cagatgatta tagtacttaa ttttgaatat gtcaacagat aaataccgat
     7381 tgtttttgat atactgtatt tgtgataatc gattctcgct cctcggataa agaaaatatg
     7441 atatactaga taaacgaaat aagagagaag gaatactatg tacgcatatt taaaaggaat
     7501 cattaccaaa attactgcca aatacattgt tcttgaaacc aatggtattg gttatatcct
     7561 gcatgtggcc aatccttatg cctattcagg tcaggttaat caggaggctc agatttatgt
     7621 gcatcaggtt gtgcgtgagg acgcccattt gctttatgga tttcgctcag aggatgagaa
     7681 aaagctcttt cttagtctga tttcggtctc tgggattggt cctgtatcag ctcttgctat
     7741 tatcgctgct gatgacaatg ctggcttggt tcaagccatt gaaaccaaga acatcaccta
     7801 cttgaccaag ttccctaaaa ttggcaagaa aacagcccag cagatggtgc tggacttgga
     7861 aggcaaggta gtagttgcag gagatgacct tcctgccaag gtcgcagtgc aagcaagtgc
     7921 tgaaaaccaa gaattggaag aagctatgga agccatgttg gctctgggct acaaggcaac
     7981 agagctcaag aaaatcaaga aattctttga aggaacgaca gatacagctg agaactatat
     8041 caagtcggcc cttaaaatgt tggtcaaata ggagcagaga atgacaaaac gttgttcgtg
     8101 ggtcaagatg accaacccgc tctacatcgc ctatcatgat gaggagtggg gccagcccct
     8161 ccatgatgac caagtattgt ttgagttgtt gtgtatggaa acctatcagg caggcctgtc
     8221 ttgggaaacg gtactcaaca aacgccaagc tttccgagaa gtctttcata gctatcaaat
     8281 tcactcagtc gcagagatga ctgacactga attggaagcc atgctggaga atccagctat
     8341 cattcgaaat agagccaagc tttttgctac acgcgctaac gcccaagtct ttctacagtt
     8401 acaggcagag tacggctctt ttgatgccta tctttggtct tttgttgagg ggaaaactgt
     8461 cgttaacgat gttcctgatt atcgccaagc gccagctaaa acacccttat ctgagaaatt
     8521 agccaaagat ctcaaaaaac gaggcttcaa gttcacaggc ccagtcgccg tattgtcttt
     8581 tctacaggct gcagggctag ttgatgacca cgagaatgat tgtgagtgga aaggtcttaa
     8641 atgatgtcta acaaaaataa gggaattctg atttttgcga ttctctatac agtcctcttt
     8701 gtgtttgatg gcgttaaatt gctggcttct ttaatgccat ctgtcattgc aaattatctt
     8761 gtttatgtag ttttagcttt atatggctcc ttcttgttca aggatagatt gatccaacaa
     8821 tggaaggaga ttagaaagac taaaagaaaa ttcttctttg gcgtcttaac aggatggctc
     8881 tttctcattc tgatgactgt tgtctttgaa tttgtatcag agatgttgaa gcagtttgtg
     8941 agactagatg gacaaggtct aaatcagtct aatattcaaa gtacctttca agaacaacca
     9001 ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg tagaagaatt atttttccgt
     9061 caggtcttat tgcattactt gcaggaacgg ttgccaggtt tactaagcat tattctggta
     9121 ggacttgttt ttgctttgac tcatatgcac agtttggctc tatcagagtg gattggtgca
     9181 gttggttact taggtggagg ccttgccttt tctattattt atgtgaaaga aaaagagaat
     9241 atctactatc ccctacttgt tcacatgtta agcaacagcc tctccttaat cattttagct
     9301 atcagtatag taaaatgaaa taagaacagg acaaatcaat caggacagtc aaatcgattt
     9361 ctaacaatgt tttagaagta gaggtgtact attctagttt caatacactg taacatgtga
     9421 tgaaaatgcc agtaatgata ccgagaaaaa agctgagaaa cttttcccag ctttatttgt
     9481 tatagtcaaa gcgaatgact tgttcctgtg catctacatg agcatggacc ccaaagggta
     9541 caattgctct tggagttgcg tggccgacat tcagattata gacaatcggg atattgctgt
     9601 caatgatatc taatagtgcc tctttatagt cgtcatggaa agtttcatcc ataggttttc
     9661 cgaccaagag tccattgatg accgcgaata tgccagtgtc ctttaaagtt agcaacatct
     9721 ttttgaagtc ttctggctta ggcttttctt cgcttgtttc gagcaagagg attttccctt
     9781 cccagtctga caagtcaggg aaaagtttgt attttgggca gagttccgtg ctatctgcgt
     9841 atcgagagtt gtcaaagata tcgtagaggg attctaggca accaccgagg attttcccct
     9901 cgaactgagc acttccttgc aacaagtcaa aacctgtatt tgtatgactg acacgaggtg
     9961 ttcccagggc cgtgggacta aaatcagttc gttcctcata ccaaacgtca ctagggcgga
    10021 tttctgaaat tcttcccgtc tcaatcaatt ctttaaagta gtgaaggcta taggctagca
    10081 tttctttgtc taattcacaa atgtctgcta aaaaggattg accataaaaa gtcttgattc
    10141 ctaatttatg caacatgagg tggttcatgg ttgtatccga gaagccaaga aaaatttttt
    10201 gcttgataac cttttggagt tggtcatttt caaaaagata aggtagcaag cgataggtat
    10261 cgtctccacc gatggcacat aggatcatgt cgatgctatc atcagaaaag gcatgaatca
    10321 aatcctctgc acgagcttca ggatggtcct tgataaagtc taatcctttt aacgaatggg
    10381 gcaaaaagat gggattgagt cccagatcct tgagacgttg gatacccaag tccacttcgt
    10441 gtttgacaaa atcctctccg ataatgccac tagataaact aacaatacca atagtagaaa
    10501 ccatatctca tcctcctaga aatagattga gcctatttta tcacaaaata taagagaaag
    10561 ctatgtggga tttcagaaga atacttttaa tactcttcga aaatctcttc aaaccacgtc
    10621 agttttatct gcaacctcaa agctgtgctt tgagcaacct gcagctagct tcctagtttg
    10681 ctctttgatt ttcattgaat ataactcaaa aaagtaagtg aaaaagcaac cgcacctgca
    10741 gttgctttta tcgttttctt attcttgata gcgtgttggt cctgcacttg cactacggat
    10801 attcaagcgt ttcttgagat agaagcgaag ggctaggagg gctgctccaa taatagctag
    10861 tggcaatgga tccagtactg ggttaaggct agctggtagg aagcttgttg caaagaagac
    10921 aagcaaccaa aggaacatag aagctaggat aactagtaca gatttccaga aaggtggacg
    10981 ttgactgcga tccatatctg gtccatagta ttggtaaaca aagtagtaca tcaagtagaa
    11041 ggcaaatcca ccaaccagtc caactaatag aagagtaatc aatccatagc cgaaagcttg
    11101 gtctgccgca aagaaggttg tgagggcgct gacaagggca aagaggctag tgatgaaaag
    11161 agctgagtcc ataatcatca gttttgggtc atcattttct tttggatgct ctttttcgta
    11221 ctgctctttg acagtgaagc tatgagccca atgagttggt gcgccgtata gggaacgggc
    11281 agttgtacct ttagattgct cctcaaggat ttggggaatg acttcctcaa aaatagtctg
    11341 gatttcagcg tctgttttcc catctttgat gaattgttgg gtagcaatgt ggacaaactc
    11401 ttggtttttc ttagttaatt tttgtagatc aatttgagac ataggaactc ctcttagaac
    11461 cattttttat ggatgagata gagagtgagc gagacactca tagcaaaggc gataaagacg
    11521 attaaccaga aggcatttgg ctctccgttt agggggattt cattatcctt aaagttcatc
    11581 ccgtaggcag aaaagaccat ggttgggatg gacatgacga tggtcacaag ggccaaggtt
    11641 ttcatgatgt tgttctggtt gttagaaatg atagaggcaa aggtctctgt catagaatgc
    11701 aagacgtttc cataaatatc tgccatctcg atggcctgtt gggtttcaat cagggtgtct
    11761 tcaagcaggt cttcgtcctc aaggtatttc ttgatattgc tggttgaact ggtcaatttc
    11821 ttaatcacgc gctcatttgt tttgagggag gccttgaaat agacgatagt tttttccaat
    11881 tccatgagct caatcaattc ttcattacga gttgattgat gcagttgact ttcgatttgt
    11941 tcactcttgc ggtcgattga acgaagggct gttaggtaaa gctctgcatt gcgataaaga
    12001 atttgaaaga taaaacgtga acgcatgaag gtatagaaat tacgcaatcg acggttgata
    12061 aagacatcaa ggacaggtag tggttccaaa cacgtagtga taatggtttc ctcagtgatg
    12121 ataataccaa gcgggatggt tacgtagtag gtgcggttat ttctttcctc cgtgaccggc
    12181 acgtctacga taatcagggt atactcgtct tcaatggtaa tacgagacat ttcttccgca
    12241 tcgagcggtg ctcgaaggtc ggcaatatca atatcgaagg tgttggcgat ttcgagtgat
    12301 tcattttgag tcggattgac gagattgatc caagtacccg gttcaagcgt atcgatctct
    12361 ttaaattcag ttgttgtaga gagaaaaact tgtttcatat cccttatcct ttctcatttt
    12421 tcagattttt tcacactgta ctattatact acaaaatcgg cctttttcaa aataaatcta
    12481 acaaaaatcc tgttgctatt ggatttttac tttgtcttga aatgttgatt tttaaatttg
    12541 ctcccctttt tgctcccccc tatagtgctt ttagtgcagt ttcgtagaat gatactgctt
    12601 tttttgcgtt ctcttttgag aggtggctat atatatccat ggtcattgat aaagtggaat
    12661 gacctaggcg gtactggagt tccttgtatg gtattccaga gttaagcaat aaactagcgt
    12721 gagtatgacg gaatccgtga aagccaatgt taggaacatc agcacgctta aaatgtgttt
    12781 gcaatcttct tttaagtctt gaactgctag gatactcatg aatgaaatca gaaaatacta
    12841 cactttcacg ttgccctatc ttccaggcct ctttagtttg gcgcagtttg tactgcttta
    12901 gcatgctaac agtcgcttga tctatatcta tatcccgata actagcctta gattttgggc
    12961 tattgatttc tatatcccga tttagtgttt tggtaacatg taccacggca ttatctaaat
    13021 ctatatcaga ccaggaaaga gctaaagctt cattgatacg gcaaccagta gccaatagga
    13081 acttatagag cgttgtctca tagtaataac ggtatctatg agtatctaaa ttatcgagat
    13141 aaccaagaaa cttttttagt tcttgatttt caaagtgttt taccttttgg cgctttgctt
    13201 tctgagtgtt acgaggtaaa acaacatcac gcgcaggatt ggacggtata gcttgcatgg
    13261 ttacgccata ctgtaagata cgtttgttta aagcgtgtat cttgtcataa tgtagaaaag
    13321 cccctttttc ccctctatta gtcttatcag caagttcgtt gataatggac tggataagtg
    13381 gagtagtgag cttatcgagc ttgtaagccc caaaaatagg caatatatga ttatcgatta
    13441 gctttctgac atttccttga gtgtttggct taactgtatt cttataactt tctaaccaga
    13501 gattggctaa ttcttgatag ttttttatgg tgctagcctt ttgtctagta gatcctgctt
    13561 tttgaaaagc tattttttct tgattggctt tctgttttaa ttccttttgt gtccgacctg
    13621 ttaccttggt tttgactttc tttcctgtta cctggtcaac tcctagatat acattagcac
    13681 gatagacttt actaccgtct ttttttataa cttcagttat tttcatgatg ataaaccttt
    13741 ctaaaataca tcagcaggca agccattatc aaaaaggttt tatatcatgc tagggcttca
    13801 tagtttgccc tgtattcgat tttaagtagt cagacggtaa ataataccag aggagggaac
    13861 aaggcggata tggctagtat tatctaaagt tttaaattcc tgttcataaa attaatagtt
    13921 aagttccata atttcattaa attgtatagt tttactaact attgtggtaa cttgggcatt
    13981 gttctatgcc agtttgcagg aaatcctaat tcagttaaaa acttatcaat tgagactgta
    14041 agtagatgtt tttcaaatgt tttaaatctt gatagtaatg tattatgtaa cttagcatac
    14101 tcaattgaac ttaaaaagca ttgtaagact ataaaaacat ggtaagggct actttttgca
    14161 caagaattac taataccata gttagaatgt aaatctgaat agtatttaac acttttctta
    14221 cacttaaagc taaaaagtct attatcatga gcgcatacat ttctaacttc tccaatattc
    14281 tctacaaaac taacaagtat agctggtgtg aaattt
//
LOCUS       DAVMHG010000044        13151 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMHG010000044 DAVMHG010000000
VERSION     DAVMHG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.044"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            40..1299
                     /locus_tag="VYR08_001051"
                     /pseudo
     CDS             40..1299
                     /locus_tag="VYR08_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1395..2669
                     /locus_tag="VYR08_001052"
     CDS             1395..2669
                     /locus_tag="VYR08_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV3466593.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2679..3557
                     /gene="truB"
                     /locus_tag="VYR08_001053"
     CDS             2679..3557
                     /gene="truB"
                     /locus_tag="VYR08_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV3466594.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3798..4100
                     /locus_tag="VYR08_001054"
     CDS             3798..4100
                     /locus_tag="VYR08_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV3466595.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMADLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4208..4846
                     /gene="udk"
                     /locus_tag="VYR08_001055"
     CDS             4208..4846
                     /gene="udk"
                     /locus_tag="VYR08_001055"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV3466596.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4974..6314
                     /locus_tag="VYR08_001056"
     CDS             4974..6314
                     /locus_tag="VYR08_001056"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV3466597.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6292..6504
                     /locus_tag="VYR08_001057"
     CDS             6292..6504
                     /locus_tag="VYR08_001057"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV3466598.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6501..7376
                     /locus_tag="VYR08_001058"
     CDS             6501..7376
                     /locus_tag="VYR08_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV3466599.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7369..8184
                     /locus_tag="VYR08_001059"
     CDS             7369..8184
                     /locus_tag="VYR08_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV3466600.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8177..8608
                     /locus_tag="VYR08_001060"
     CDS             8177..8608
                     /locus_tag="VYR08_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3466601.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8615..10282
                     /gene="recN"
                     /locus_tag="VYR08_001061"
     CDS             8615..10282
                     /gene="recN"
                     /locus_tag="VYR08_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV3466602.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10284..11012
                     /locus_tag="VYR08_001062"
     CDS             10284..11012
                     /locus_tag="VYR08_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV3466603.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11091..12914
                     /gene="lepA"
                     /locus_tag="VYR08_001063"
     CDS             11091..12914
                     /gene="lepA"
                     /locus_tag="VYR08_001063"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV3466604.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13073..13151)
                     /locus_tag="VYR08_001064"
                     /pseudo
     CDS             complement(13073..13151)
                     /locus_tag="VYR08_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         3902 a         2428 c         3157 g         3664 t
ORIGIN      
        1 gctacaatat ccttaataaa ataatatgga ggtcacctta tgacttgtga ttttaaattt
       61 gaaactctac aactacatgc tggtcaagtt gtggctccag ctactaagtc tcgtgcagtg
      121 ccgatttatc aaacaacatt ttttgttttt gatgacacgt aggaaggtgc cgatctgttt
      181 gccttgagga aaccagggaa catttatact cgtatcacca atcctacaac agctgccctt
      241 gaaggtggtg ttgaagcgct agcaacagca tcaggtatga ctgcagtgac ttatacgatt
      301 ttggcgcttg cccatgctgg tgaccatgta gtgactgctt cgactattta cggtggaacc
      361 ttcaatcttt tgaaagaacc ccttcctcgt tatggtatca caacaacctt tgtcgatgtt
      421 gataatttgg aggaagtaga agcagctatc aaagacaata ccaagcttgt cttgattgaa
      481 accttgggta actccttgat taatattcca gacctggaaa aactggcaga gattgctcat
      541 aaacatcaaa tcccacttgt gtcagacaat acttttgcaa taccttattt gattaacgtc
      601 ttctctcatg gcgttgacat tgccattcac tctgcgacta agtttatcgg tgggcatggt
      661 acaactattg gaggaataat tgtcgatagt ggtcgttttg actggacggc ttcagggaaa
      721 ttccctcaat ttgttgacga gggtccaagc tgccacaatt tgagttatac ttgtgatgtg
      781 ggtgcagcag cctttattat agctgttcga gttcaattgc ttcgtgatac aggtgcagcc
      841 ttgtcaccat tcaatgcttt cctcttgcta caaagacttg aaacctcttc acttcgtgtg
      901 gaacgccatg tacaaaatgc tgagacaatt gttgattttc ttgtcaacca tcctaaggta
      961 gaaaaggtaa attatccaaa acttgcagat agtccttatc atgccttggc tgagaaatac
     1021 ttgccaaaag gtgtcggttc aatctttacc ttccacgtca aaggtgacga ggaagaagca
     1081 cgcaaggtca ttgatcattt agaaatcttt tctgaccttg caaacgcggc agatgctaaa
     1141 tcgcttgttg tccatccagc aacaaccact cacggtcaat tgtcagaaaa agacctagaa
     1201 gcagcaggtg tcacaccaaa ccaaattcgt ctgtcaatcg gtcttgaaaa tgtagaagat
     1261 ttgattgaag acttacgctt ggccttggaa aaaatttaaa gtaaaagaag ataaacagtg
     1321 ggttttgact cactgttttt gattttccct caggcatgat ataatggtta cagaagtcta
     1381 gaaagaggaa tgtaatgaac gaaatcaaat gtcccaactg tggggaagtc tttacagtaa
     1441 atgaaagcca gtatgctgaa cttttgtccc aagtgagaac ggcagagttt gataaggaac
     1501 tacacgatag gatgaggcag gaactggcct tggctgagca aaaggctatg aatgagcaac
     1561 agactaaact ggctcagaag gatcaagaaa ttgcgcaatt gcagagtcaa atccaaaact
     1621 ttgatacaga aaaagaattg gccaagaaag aagttgaaca gacaagccat caggccttat
     1681 tggctaagga caaggaagta caggctttgg agaaccaatt ggccactttg cgtttggagc
     1741 atgaaaatca actgcaaaag accctttctg acctagaaaa agaacgcaat caggtcaaaa
     1801 atcagctcct actgcaagaa aaggaaaatg agttgtcttt ggcttctgtt aagcaaaact
     1861 acgaagccca gctcaaagca gccagtgaac aagtcgagtt ttataagaat tttaaggctc
     1921 aacaatctac aaaagcgatt ggggaaagcc tagaacagta tgcagagagt gagtttaaca
     1981 aggtccgtag tttcgccttt ccaaatgctt actttgagaa ggataacaag gtctcttcgc
     2041 gtgggtctaa aggggacttt atcttccgtg agtgtgatga aaatggagtt gaaatcattt
     2101 ctatcatgtt tgagatgaaa aacgaagcgg acggaacaga gaagaagcac aagaatgcag
     2161 atttttacaa ggaattggac aaggaccgtc gggagaagaa ctgtgagtat gccgttttgg
     2221 tgaccatgct tgaggccgat aatgactact ttaacacagg gattgttgac gtcagccacg
     2281 agtatgaaaa aatgtatgtt gttcgtcctc aattctttat ccagttgatt ggtctcttac
     2341 gtaatgcggc gctaaattcc ctaaaataca agcaggagtt ggccttggtt cgcgagcaaa
     2401 atattgacat tacgcatttt gaggaagact tggatgcctt taagctagct tttgctaaga
     2461 actataattc agcttcgact aactttggaa aagctattga tgaaatcgac aaggccatca
     2521 aacgcatgga agaggttaag aaattcctga ctacatctga aaaccaactc cgtctagcta
     2581 acaacaaatt ggaagatgtc tctgttaaaa aattgacccg gaaaaatcca acaatgaaag
     2641 cgaagttcga agcattgaag ggggagtaga aagcaaaaat gaacggtatt atcaacttaa
     2701 aaaaagaagc aggaatgacc tcgcatgatg cggtttttaa actgcgtaag attttgggaa
     2761 ccaagaaaat tggtcatggc ggaaccttgg atccggatgt ggtgggtgtt ttgccgattg
     2821 cggttggcaa ggcgacacgc atggtcgagt ttatgcagga cgagggtaag atctatgagg
     2881 gggaaatcac tctgggctat tccacgacga ctgaggatgc tagtggggaa gtggtcgcag
     2941 aaacccctgt tttgtctccc ttggatgaaa agcttgttga tgaagcgatt gctagcttga
     3001 ctgggcctat tacccagatt ccccctatgt attcggcagt taaggttaat ggtcgcaagc
     3061 tctatgagta tgcgcgtgct ggtcaggaag tggagcgtcc agaacgtcag gtgaccattt
     3121 atcaatttga gcgaacaagt ccgatttctt atgatggcca acttgcccga ttcacttttc
     3181 gtgtaaaatg cagtaaaggg acttacatcc gtactttgtc agttgatttg ggtgaaaagc
     3241 ttggttatgc ggctcatatg tcccatttga ctcgtactag tgctgctggc ttacaattag
     3301 aagatgctct taccttggag gaaattgctg aaaaagtaga ggctgggcaa ttagattttc
     3361 tccatccttt agagattggg acaggtgacc ttgtcaaagt tttcctaact ccagaagatg
     3421 ctacagaagt tcgctttggt cgttttattg agctagacca aacggacaaa gaactggctg
     3481 cctttgaaga tgataaattg ttagccattc tagaaaaacg gggcaatctc tataagccaa
     3541 ggaaggtttt tagctagatc gtttaggaat aaaaatcggg tgatagataa caattgcttg
     3601 ataaaacccc atactaatag tagaatggtt ttgggaatta taatattcca attgttgcga
     3661 gttgtagata ctcaaataat ctatatagaa atttagaggt gtgaaatgaa gcaacttaaa
     3721 attctttcag ataaatattt agagtccatt acaggttctg gtgggaactt aggcccagga
     3781 tttggtgtga taattccatg atgcgaaatg agtttcgaga aagggtggag caacttcttc
     3841 aacaaaaaga aataaatgaa aatagtgagt tgagtcacct gtttcgtctt gctatacaaa
     3901 atttagacag aaatgaaaaa taccaaacgg tcatggccga tttgagtcaa gggttgtcac
     3961 tttacctcat gacgcatcat taccaggcac ctaagtctgt cattgatttt ggtttatgga
     4021 ttgccaaagc tcctagccag gaaagaggga gactggcttt cttgcagatg cttgctcaaa
     4081 ccctacaggg ctttcgttaa aactgtcgaa catcagaggg aagactctgg tgttttttgc
     4141 tgatgagact ataaattttg taaaaaatgt gatagaatag acgacggata aaataacgga
     4201 ggatagcatg caaaatagac caatcattat tggagtgaca ggtggttctg gtggtggtaa
     4261 aaccagtgtt tcaagagcca ttttatcgca ttttcctgat gaaaagattt ccatgattga
     4321 gcatgattca tactacaagg atcagtctca tttgaccttt gaagagcgtg tcaaaaccaa
     4381 ctatgaccat ccttttgcct ttgatacaga cttgatgatc gagcagatta aggaattgtt
     4441 ggcagggcgt ccggtggaca tcccgaccta cgactataca gagcatacac ggagtagcaa
     4501 aacctatcgt caggagcctc aagatgtctt tatcgttgag ggcattttgg tcttggagga
     4561 caagcgtctg cgcgatttga tggatatcaa gatttttgtg gatacggatg acgatgtgcg
     4621 cattattcgt cgtatcaagc gtgatatgga ggagcgtggc cgtagccttg atagcgttat
     4681 taaccagtac ttaggtgtgg tcaaaccaat gtaccaccag tttatcgagt caactaagcg
     4741 ttatgctgat atcgtcattc ctgaaggggt tagcaatacc gtggctatcg acctgttgac
     4801 gaccaagatt gcaaagattt tggaagaagc tcgaaacagc aaataatcac atgtggaggc
     4861 cttgcctcct ttttctattt ttccctcata atggtacata aatggagatt tttaggcata
     4921 tttttggtat aataataccc ataaaagagc aagtaaatga atagtaggtg gagatggaaa
     4981 agtatttatc ggtaacaact ttgaccaagt atctgaaaat gaaattcgat aaagacccat
     5041 acttggaacg ggtctattta actggtcaag tttccaactt tcgtaaacga cctactcacc
     5101 aatatttctc cctaaaggat gaccatgcag ttattcaagc gaccatctgg tctgggattt
     5161 atcagaaatt agggtttgac ctggaagaag gaatgaagat caatgtgatt gggcgtgtac
     5221 aggtctatga accaagtggt agctactcca tcatcattga aaaagctgag cctgatgggg
     5281 ttggggcgct tgcgattcag tttgaacaac ttaagaaaaa attgacagaa gaaggcctgt
     5341 ttcaagaacg cttcaagcaa gctctacccc aattttctaa gagaattggg gtagtaacca
     5401 gccgtagtgg agccgttatt cgagatatta tcacgaccgt cagcaggcga ttcccaggtg
     5461 ttgacatcct tctttatccg accaaggttc aaggtgaagg ggctgcggag gaaattgcta
     5521 gaaatattgc gcgtgctaat caacgggatg atttggattt gctcattatt ggtcgtggag
     5581 gtggttctat tgaggatctc tgggccttta acgaagaaat tgtggtacga gctatttttg
     5641 aatctcgttt gccagttatc tctagtgtgg ggcatgagac ggatgtgacc ttggcagatt
     5701 ttgtggcaga tcgacgcgct gcaacgccaa cagcggcggc tgaactggca acacctgtga
     5761 ccaagttgga tgtattagct catttgcaaa atcaggaaaa acggatggca acggcagtcc
     5821 gaaatgttct atctaagaaa caagaggctt tgaaaaaatg cagtcagtct gttatcttta
     5881 gacaacctga gcgcttgtat gacggttatt tgcaacgctt ggaccaactg caactgcgtt
     5941 tgaaacaaag tttgcgaact cggatttctg ataacaaaca attagttcaa gcaagaactc
     6001 atcaattagt acaattatca cctgttacca aaatccaacg ctatcaagac cgtttaggac
     6061 agttggacaa gctcttaggt agccaaatgg cgttagttta tgacgccaag gttgctgagg
     6121 ccaagcgact ttcggaagct ttgctcatgt tggatactag ccgaatcgtg gcgcgtggtt
     6181 atgctattgt caaaaaagaa gagtccattg tagattcggt tgagagtttg aagaaaaaag
     6241 accaagtaac gcttttgatg cgagatggtc aagtagaatt agaggttaaa gatgtcaaaa
     6301 caaaagaaat ttgaggaaaa tctagcagaa ctggaaacca ttgtccaaag tttggaaaat
     6361 ggtgaaattg ctctggaaga tgcgattact gcctttcaaa agggcatggt cttgtcaaaa
     6421 gagctccaag ctacgctgga caaggctgaa aagaccttgg tcaaggtcat gcaagaagac
     6481 ggaacagaaa gtgattttga atgaaaaagc aagaaaaatt agctcttgtc gagtcggctt
     6541 tggaagattt ttatggagac cagcagtttg cctctagttt acgggagtct gttctctatt
     6601 ctattcatgc tggtggcaag cgtattcggc cttttctctt gttagaagtt ctggaatcct
     6661 tgcaggttgc catcaaacct gctcacgcgc aggtagctac tgccttggag atgattcata
     6721 cagggagctt gattcacgat gaccttcctg ctatggatga tgacgattat cgaagagggc
     6781 ggttaaccaa tcacaagaaa ttcggtgaag ctatggccat tttggctgga gatgccttat
     6841 tcttagaccc atatgccttg attgcgcagg cagatttgcc aagtcagatc aaggtggact
     6901 tgattgccaa cttatccctt gcttcaggta gtctgggtat ggtggcaggg caagttttgg
     6961 atatggaggg cgaacaccag cacttgtctc tggaagaact tcagactatt catgccaata
     7021 agactgggaa gttactagcc tatcccttcc aagcggcagc tattatagct gaattgtcac
     7081 ctgaaatgca ggtgaagctg aaaactgtgg gtgaattgat tggacttgct tttcaagtca
     7141 gagatgatgt actggatgtg acagctagtt ttgaggaaat cggcaagaca cctcaaaagg
     7201 atctgcaggc agaaaaatca acctatcctg ccttgttggg cttggaagag tccattgcct
     7261 tttgtaacca aaccctggat caagctaatg aaaaattaga agagattgcc cagcagcttc
     7321 cctttgaaac agaatcgatt gtaagtgtag tagaaagttt gagaatcaat ggctaaggaa
     7381 agagtggatg tactagctta taaacagggg ttgtttgaaa cgagagagca ggccaagcga
     7441 ggtgtgatgg ctggtctagt cgtagcagtc cttaatggag aacggtttga caagccagga
     7501 gagaaaattc cagatgacac cgaattaaaa ctcaaggggg agaaactcaa gtatgtcagc
     7561 cgtggtggtt tgaaactgga aaaggccttg caggtctttg atttgtcggt ggatggcgcg
     7621 actacgattg atatcggggc ctctactgga ggttttaccg atgtcatgct acagaatagt
     7681 gccaagttgg tctttgcagt cgatgttggt accaatcagt tggcttggaa attacgccaa
     7741 gacccacgag ttgtcagcat ggagcagttc aattttcgct atgctgaaaa gactgatttc
     7801 gagcaggagc cgagctttgc cagtattgat gtgagtttca tttcccttag tctgattttg
     7861 ccagccttgc accgtgtctt ggctgatcaa ggtcaggtgg tagcacttgt caaacctcag
     7921 tttgaggcag gacgtgagca gattgggaaa aatggaatta ttcgagatgc taagattcat
     7981 cagaatgtcc ttgaatctgt aacagctatg gcagtagagg caggtttttc agtccttggc
     8041 ttggactttt ctcccatcca aggtggacat ggaaatattg aatttttagc gtatttgaaa
     8101 aaagaaaagt cagcaagcaa tcagattctt gctgagatta aagaagcagt agagagggcg
     8161 catagtcaat ttaaaaatga ataaaaaaga aagacttgaa aaaattagaa gattggtgac
     8221 agattatcaa atcggcacgc aagaagaaat tgtagaacat ttgaaagaag caggtatcac
     8281 tgccactcag gcgacggtat cccgagatat caaagagtta ggtattgtca aaattccttt
     8341 gagagacaac acctatgtct acgaattgcc aaaatcaatc gtaaaaagtc tgcaactggc
     8401 tgaagacaat atcgaatcgg ctgaattgat ggataagatg atcaatctcc aagttattcc
     8461 aggaaatacg gcttttgtaa aagctcagtt aatcgagact tttgcagaca agatttttag
     8521 ctgtttgact gatgatagct cgattttagt cattgccaga agtggaagtc tagcagagga
     8581 aatctttgaa caagtaaaaa attggtaggt ctatatgtta cttgaaattt cgataaaaaa
     8641 ctttgccatt attgaggcta tttccctcaa ttttgaaaag gggatgactg tcctgactgg
     8701 tgaaacgggt gcagggaagt cgattatcat tgatgccatg aatatgatgt tgggagctcg
     8761 tgcgacgaca gatgttattc gtcatggtgc accaaaggca gagattgagg ggcttttctc
     8821 agttgagaat agtcgtcttt tacaggaaat ttttgatgag caaggtttag aattaggtga
     8881 tgaaattatc atccgtcggg aaatcttgca aaatggtcgt agtatcagcc gtgtaaatgg
     8941 tcagatggtt aatctgtctg ttttgcgaac cattgggcaa catcttgtag atattcatgg
     9001 tcagcatgac catgaggagt taatgcgtcc ccaactgcat atccagatgt tggatgaatt
     9061 tggtgatgcc gctttttggg acttgaaaga aacctatcaa acgagttttg atgcctatcg
     9121 gaaaatgcgc aagcaggttc tggaagtcaa gaaaaaccaa caggaacaca aggcacgtat
     9181 cgaaatgttg gaatttcaaa tggcagagat tgaggcagca aacttgcagg ctggagaaga
     9241 cttggatctc aatcaagagc gagataaact cctcaaccac aaaaatattg cggatacact
     9301 gaccaatgcc tacagtatgt tggacaatga agatttttca agtctggcca atgttcgttc
     9361 agccatgaat gacatggaaa gtgtcgaaga gtatgaccct gaataccgtg aaatttcaag
     9421 ctctctgtct gagacctact atgttttaga agacattagc aaacgtttgg aagctatcat
     9481 tgaggacctt gattttgatg gcaatcgcct catgcaggtt gagaatcgtt tggacctcct
     9541 tcatactatt actcgtaagt atggtgggac tgttgatgat gttttgcttt attttgccaa
     9601 gattacggaa gaatacaatc tcttgacagg caataatctt tcgtctgagg acatggaagc
     9661 agagcttaag aagttggaag tcaatcttgt caatttggca ggtcaacttg cttctgctcg
     9721 tcataatttg gctcagcaac tcgaagctga gattaaacaa gaactgcaag atctttatat
     9781 ggaaaaagcc cagtttcagg ttcgttttag taagggaaaa ttcagtcgtg agggaaatga
     9841 aatggttgag ttttatattt caaccaaccc tggagaagac tttaaaccct tggttaaggt
     9901 tgcttctgga ggggaattat ctcgtctcat gttagccatt aagtctgcct tttcacgtaa
     9961 agaaggcaag actagcattg tctttgatga agtggatacg ggagtttcag gtcgtgttgc
    10021 tcaagctatt gctcagaaaa ttcataaaat tggtcagcat ggtcaggttt tggctatctc
    10081 ccatttgcca caagtaattg cgattgcaga ttatcaattc tttattgaga agattagtaa
    10141 tgaccattca acggtttcga ctgttcgtct cttgacggtc gaagagcgag tggaggaagt
    10201 tgccaagatg ttggcaggtg atgatgtgac agaagcagcc ctgacgcaag ccagagaatt
    10261 gttgagaaac agggagaaat aagatgacag attattatgt aattggagat gttcacggaa
    10321 aagctgggat gctggaagac cttcttaaaa cctgggatgg tcagacccag ttgctctttc
    10381 taggtgattt gattgacaga ggtgaggata gtcaccgtgt tcttgaaatg gtcaaggact
    10441 tggtggacaa tcaaggggct atctgtttgt ctggaaacca cgagtatatg tttttgactt
    10501 ggctagatga cccagaagaa agttatgacc attatcgtcg caatggtgga gatacaacca
    10561 ttaactctat cctaggtcgt cccttggatg caccagttga tggagtagaa gatgccaagc
    10621 gggttgccgc tgaagcggca gacttggtcg aattcattcg tcaaatgcca tttgtagtag
    10681 agacagacaa gtatatcttt gttcacgcag gtattgattt gaccttggat gactggcatg
    10741 aaacaacaga ttataaaaaa gtatggctta gaaaaccatt ccacggagct gaaaatcata
    10801 ctggaaaaac cattgtcttc ggtcatacac cggtctatgg tttgttacag caagaacgcg
    10861 gtacatctga gctttggact acagaagatg gcaagattgg aatggatgga ggagctgttt
    10921 atggtggtgt ccttcacggg attgtcttta cagaccaagg aatgacagaa caccacttta
    10981 tcgaaaatga cggctttgtc gctgaagatt agtactccta gcagggtatg gtcttgtcaa
    11041 aatatcaaaa acaatttata ataaatagat accctgaaag gaagagaatc atgaacttag
    11101 aagaattgaa aaaacgacag gagaagatcc gtaacttctc tattatcgcc catattgacc
    11161 atggaaaatc aactctagca gaccgcattt tggaaaaaac agagacggtt tcaagtcgtg
    11221 aaatgcaggc ccagcttttg gatagcatgg agctagagcg ggaacgtgga attactatta
    11281 agttgaatgc catcgagttg aattacactg caaaagatgg ggaaacttat attttccatt
    11341 tgattgacac accagggcac gttgacttta cctatgaagt ttcacgttcg ctagctgcct
    11401 gtgagggtgc tattttggtg gtcgatgcgg ctcaaggaat tgaggctcaa actcttgcca
    11461 acgtttatct ggctttggat aatgatttgg aaatcatgcc tatcattaat aaaattgacc
    11521 tgccggctgc agatccggag cgcgtgcgta cagagattga agatgtgatt ggtttggatg
    11581 ccagtgaagc agttttggct tctgccaagg ctggtattgg gattgaagaa atcctcgagc
    11641 aaattgtaga aaaagtacca gcaccaacgg gtgatgtgac ggcgccactt aaggccttga
    11701 ttttcgactc tgtttacgat gcttaccgtg gggttatcct ccaagtgcgt gtcatggacg
    11761 gagtggtcaa acctggtgat aagattcagc tcatgagcaa tagtaagacc tttgatgtgg
    11821 ccgaagtcgg tatttttaca ccaaaagcgg ttggtcgtga tttccttgcg actggtgatg
    11881 ttggttacat tgcggcgtct atcaagacgg ttcaggatac tcgtgtgggt gataccgtta
    11941 ccttggcaac caatcctgcg gcagaaccat tacatggtta taagcagatg aatcctatgg
    12001 tctttgcggg tctctaccca atcgaatcaa acaaatacaa tgacctgcgt gaagcgcttg
    12061 aaaaattgca actgaatgat gctagtcttc agtttgaacc agaaacatct caggcacttg
    12121 gatttggttt ccgttgtgga tttcttggac ttctccatat ggatgttatc caggaacgtt
    12181 tagagcgtga gttcaatatt gacctcatca tgacagctcc gtctgttatt tacaaagtta
    12241 atttgaccga cggtgagtct atggatgtgt ctaacccatc tgagtttcca gacccaacta
    12301 agattgcgac cattgaagag ccttatgtca aggcgcaaat catggtacca caggagttcg
    12361 tcggagcagt aatggagcta gctcagcgta agcgtgggga ctttgtgact atggactata
    12421 ttgatgataa ccgtgtcaat gttatctatc aaattcctct tgctgaaatt gtctttgact
    12481 tctttgataa acttaagtct tcgacacgtg gttatgcaag ctttgactac gaattgtcag
    12541 aatatcgccc atctaagctg gtgaaaatgg atatccttct caatggagat aaggtggatg
    12601 ccctcagctt tatcgttcac aaggactttg cctacgaacg tgggaaactc atcgttgata
    12661 aactcaagaa aatcatccct cgccaacaat ttgaagttcc aatccaagcg gctattggac
    12721 acaagattgt cgctcgtact gatatcaagg cccttcgtaa gaacgtactt gctaaatgtt
    12781 atggtggtga cgtttctcgt aagcgtaaac tccttgaaaa acaaaaagct ggtaagaaac
    12841 gcatgaaatc catcggatca gttgaagttc cgcaagaagc cttcctcagc gtcttgagta
    12901 tggatgaaga ataagttatc agaaatgcct ttccttgttc acaggaaatt tatataagaa
    12961 tcaatagtaa agcaggctta gatggtctgc ttttttagtt tttatactct tcgaaaatct
    13021 cttcaaacca cgtcagcgtc gccttactgt atgtatggtt actgacttcg tcagtttcat
    13081 ctacaacctc aaaactgtgt tttgagtaac ctgcggctag cttcctagtt tgctctttga
    13141 ttttcattga g
//
LOCUS       DAVMHG010000045        13054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMHG010000045 DAVMHG010000000
VERSION     DAVMHG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.045"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            249..641
                     /locus_tag="VYR08_001065"
     CDS             249..641
                     /locus_tag="VYR08_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466605.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            663..1454
                     /locus_tag="VYR08_001066"
     CDS             663..1454
                     /locus_tag="VYR08_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466606.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIVTYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLEKYEDNV"
     gene            1447..2088
                     /locus_tag="VYR08_001067"
     CDS             1447..2088
                     /locus_tag="VYR08_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466607.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            2092..2724
                     /locus_tag="VYR08_001068"
     CDS             2092..2724
                     /locus_tag="VYR08_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3466608.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2752..3039
                     /locus_tag="VYR08_001069"
     CDS             2752..3039
                     /locus_tag="VYR08_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466609.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            3185..5698
                     /gene="secA"
                     /locus_tag="VYR08_001070"
     CDS             3185..5698
                     /gene="secA"
                     /locus_tag="VYR08_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV3466610.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5779..6810
                     /locus_tag="VYR08_001071"
     CDS             5779..6810
                     /locus_tag="VYR08_001071"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3466611.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6812..7843
                     /locus_tag="VYR08_001072"
     CDS             6812..7843
                     /locus_tag="VYR08_001072"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV3466612.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7882..8250
                     /locus_tag="VYR08_001073"
     CDS             7882..8250
                     /locus_tag="VYR08_001073"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV3466613.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8240..9343
                     /locus_tag="VYR08_001074"
     CDS             8240..9343
                     /locus_tag="VYR08_001074"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV3466614.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9362..11377
                     /gene="recG"
                     /locus_tag="VYR08_001075"
     CDS             9362..11377
                     /gene="recG"
                     /locus_tag="VYR08_001075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV3466615.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLAFLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11694..12674
                     /locus_tag="VYR08_001076"
     CDS             11694..12674
                     /locus_tag="VYR08_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV3466616.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3825 a         2407 c         2962 g         3860 t
ORIGIN      
        1 aggagggaaa aagtcatggg ctttaaaaaa tatttgaaga atttaccgaa aaactctgga
       61 tttttgattt ggagttggat tcaacttatc tggtttgaaa catggttttg gggataaaga
      121 aattagagtc tatatataat aagtaaagga ggaatttgta tgatatacca tcgtttagaa
      181 taagtctata tttaaaatag aagttataag gaatcttctt gaataaaaaa tgaaaggaaa
      241 cagaaaaaat gaaaattaag aaattattga aaatggttat tcctgtttta atgataagtg
      301 ctgttggaac tactttcgta gaagcaaatc agataggtgc ttttagtaat tttgttatta
      361 ctaccagtta taagagaaca ggttatttga ctaaagaaaa tgaaggtgcg gaatatatca
      421 tgaacttaaa tccttgtaga aatttacatc ccatgactgt taagcatcgt atagtaaatt
      481 ctaatggaga agctcgtagt ggagaatcgt taacaacctg tggtactcgt tcaactcatg
      541 gtaattgggc aacggttggc tatgtttatg cagcagatat ggcaagacaa aattggtggg
      601 atttatcagc tgccatttca ggtagttggt caccaaatta attcgtaaag taggtataat
      661 ttatgaagat tcctctctta actttggcaa ggcataaatt tgtttatgtc ttgcttactt
      721 tgctttttct tgctttggtt tatcgtgacg ttttgatgac ttatttcttt tttgatattc
      781 atgcgcccga tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag
      841 cattagattt tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa
      901 tcatcattgt tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga
      961 gtattggaag agaagtgagt tatcaagggt taaaaagaaa gttgactttg caagttgcaa
     1021 gtatcccttg tttgatatat ttagtgactg tgctgataat tgcaattgta acctatttct
     1081 ttgggacttt ttctcctctt ggatggaatt ctctattttc tgatggaagt ggtttacaaa
     1141 gactcctaga tggagagata aaaagctatt tgttctttac ttgtgtccta ctaatcggta
     1201 ttttcatcaa tgcaatctat tttttacaaa tagttgatta tgtggggaat gtgactcgtt
     1261 cggcaatcac ctatttgatg tttctttggc ttggttctat gctgctctat agtgccttgc
     1321 cttactatat ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga
     1381 tgaaactctt tactccttat atcctttatg ttgtccctta tatggtgctt gaaaaatatg
     1441 aagataatgt ttaagaattt taacaatatt tggctaaaga gaaagattgt tttactactt
     1501 cgtatagttc tgatgatgat tttgataaac catctattgt caacagcggt tcaaaagcag
     1561 gatgctgtta tctttttcaa gagagaattg atttcaattt tttcctataa tgactattct
     1621 gaagcgaatt tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg
     1681 ctctctgtca ttttacttga aagtgatttg gcagaccatt accatcactt gattcgctat
     1741 caatcaagct cctttttcga ttatacaagg aaacgattgg ttgtcatttc taaatttttt
     1801 actcaagatt tgtttgtctg gtttcttggt ttacttccgc taggaattca tttcaaaaca
     1861 gtcgcacttt tctttttact tgctcagtta atgattttgt acttactact gtcttatctg
     1921 atagcactga ttagtgcggg cgctggtttt tccttttttc tctatttttt agcatttgtg
     1981 ggacaagaat ggatgatgga tcatattgta acagtgtatt tactactctt aagcttatta
     2041 gttatgttga ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt
     2101 gaaattataa atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg
     2161 gtgtttcaat caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt
     2221 cttttaaagg tacttgctgg ctatattaag cttgacaaag gaaaagttct tcaagacggg
     2281 aaagtctacg ggataaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc
     2341 gagtttttat ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct
     2401 aaagttacgg aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa
     2461 gacgttgaat accgtcattt atccttggga acaaagcaaa aaatggcctt gattcaagcc
     2521 tttatttcct ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag
     2581 agtgtgaggt taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt
     2641 atcctgacgt cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc
     2701 gaaaatggac atatacaaat gtaaaggata tacaatccta ggagatggct tatggcacat
     2761 ctaaaatcat ttattacacg ctattccaag gtttatattg gtttagttct gctgatctgg
     2821 ctgtctttct tctttatccc ttgggataga gcgattctgg gggtaaggat tgacatcttc
     2881 atcatacaga aaatcttgct agcttttgga attctgtcca ttctcatggc cttgctgtcc
     2941 aagaaagtca gtctctttgt ttttggactg atttgctgtc tttctctttg gattaactta
     3001 tttatcacat ttgccatttt gccgattttt ggcaattaaa cagtcataaa agtcggagag
     3061 gttagcttga aaactaacct ctttttcctt ttcaaaatgg ggattcttcc ttgaaaataa
     3121 tcagtaattg tgctaaaatt aaaggaacat tctaaaatat tcggaattta aagtaaggaa
     3181 aaacatggct aatattttaa aaacaattat cgaaaatgat aaaggagaaa tccgtcgtct
     3241 ggaaaagatg gctgacaagg ttttcaaata cgaagaccaa atggctgctt tgactgacga
     3301 ccaactaaaa gcaaaaacag ttgaatttaa ggaacgttat caaaatggag aatcactgga
     3361 ttcattgctt tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg tcctaggtct
     3421 cttcccttat aaggttcagg tcatgggggg gattgttctt caccatggtg acgtgccaga
     3481 gatgcgtaca ggggaaggga aaaccttaac tgcgaccatg ccggtatacc tcaatgccct
     3541 ttcaggtaaa ggggttcacg tagttacggt taatgaatac ctgtcagaac gtgacgcgac
     3601 tgagatgggt gaattgtact cttggcttgg tttgtcagta gggattaact tggctaccaa
     3661 atctccaatg gagaaaaaag aagcctatga gtgtgatatt acttactcaa ctaactcaga
     3721 aatcggattt gactaccttc gtgacaacat ggtcgttcgc gctgaaaaca tggtacaacg
     3781 tccgcttaac tatgccttgg tcgatgaggt tgactctatc ttgattgacg aggctcgtac
     3841 acctttgatt gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc acatggcaga
     3901 ccactatgta aaatctttga acaaagatga ctacatcatc gatgtgcagt ctaagactat
     3961 tggtttgtct gattcaggga ttgacagggc tgaaagctac ttcaaacttg aaaacctcta
     4021 tgacatcgaa aacgtggctt tgacccactt tatcgataac gcccttcgtg ccaactacat
     4081 catgcttctc gatattgact atgtggtgag cgaagagcaa gaaatcttga ttgtcgacca
     4141 atttacaggt cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc aagctattga
     4201 agccaaagaa ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa tcacgtacca
     4261 aaaccttttc cgtatgtaca aaaaattgtc tggtatgacg ggtacaggta agactgagga
     4321 agaagaattt cgtgaaatct acaacattcg tgttattcca atcccaacaa accgtcctgt
     4381 tcaacgtatt gaccactcag accttcttta tgcaagtatc gaatctaagt ttaaagcggt
     4441 tgtcgaagac gttaaggctc gttaccaaaa gggtcaacct gtcttggttg gtacagtagc
     4501 ggttgaaact agtgactaca tttctaagaa attggttgca gctggtgttc ctcacgaagt
     4561 cttgaatgcc aaaaaccact atagagaagc ccaaatcatc atgaatgctg gtcaacgtgg
     4621 tgccgttacc atcgcaacca acatggcggg tcgtggtacc gacatcaagc ttggtgaagg
     4681 tgttcgtgaa cttggaggac tttgtgttat tggtacagaa cgtcatgaaa gtcgtcgtat
     4741 cgataaccag cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt cacaattcta
     4801 cctatctctt gaagatgatt tgatgaaacg ttttggttct gaacgcttga agggaatctt
     4861 tgaacgtctc aacatgtctg aagaggccat tgagtctcgc atgttgacgc gtcaggttga
     4921 agcagctcag aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag tccttcaata
     4981 cgatgatgtc atgcgtgaac aacgtgagat tatctatgct caacgttacg atgtcatcac
     5041 tgcagatcgt gacttggcac ctgaaattca gtctatgatc aaacgcacga ttgaacgtgt
     5101 cgttgatggt catgcgcgtg ccaaacaaga tgaaaaacta gaggcaattt tgaactttgc
     5161 taagtacaac ttgcttcctg aagattctat tacgatggaa gacttgtcag gcttgtctga
     5221 taaggccatc aaggaagagc ttttccaacg ttccttgaag gtttacgata gtcaggtttc
     5281 aaaactacgc gatgaagaag cagttaaaga attccaaaaa gttttgattc tacgagtggt
     5341 ggataacaag tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg cggttggact
     5401 tcgtggctat gctcagaaca accctgttgt tgagtatcag gcagaaggtt tccgtatgtt
     5461 taatgatatg attggttcga ttgagtttga tgtgacacgc ttgatgatga aagcacaaat
     5521 tcatgaacaa gaaagaccac aggcagaacg tcatatcagt acaacagcga ctcgcaatat
     5581 cgctgctcac caagcaagta tgccagaaga tttggatttg agccagattg gacgcaatga
     5641 actttgccca tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa gacaataaaa
     5701 tgagatagtt tagaggcgga tatcttgtga aaagtaaatt tttactgggt atccgtttgc
     5761 tttataagga gatgagttat ggtatttaca gcaaaaagct ctaaaataaa tatagaagaa
     5821 gttcgtgcct tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca gcgagatcaa
     5881 gagctagaag ccattatacg tggagaagac cagcgaattc tcttggtaat cgggccatgc
     5941 tcatctgaca acgaagaagc tgtccttgaa tacgctaagc gtttggcagt cctacaagaa
     6001 gaagtggcag atcgtatctt tatggttatg cgtgtttata ctgccaaacc ccgtaccaac
     6061 ggagatggct ataagggctt gattcaccag cctaacgcga cagaagcgcc tagtcttatc
     6121 aatggaatca aagccgttcg ccatcttcac tatcgtgtca tcacagaaac agggatgaca
     6181 actgctgatg aaatgcttta tcctgaaaac cttccgcttg tagatgattt gatttcttac
     6241 atggcagttg gtgcccgttc agttgaagac cagcaacacc gctttgtggc aagtggggca
     6301 ggattttcta ctggttttaa aaatccaacc tctggaaatc tcaatgtcat gtttaatggg
     6361 atttatgctg ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt agaaacaact
     6421 gggaacccgc tttcacacgc tattcttcgt ggtgctctta atgagtatgg aaaaaatatt
     6481 cccaactact attatgacaa tttaattgat accattgccc agtatgagaa aatgggcttg
     6541 gaaaatcctt ttatcatcat tgataccaat catgacaatt ctggtaagca gtatattgaa
     6601 cagatccgaa ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa aattaagcag
     6661 ttcgttcgtg gttttatgat tgagtcttat ctggaagatg gtcgacaaaa tgagccagaa
     6721 gtatttggta agtctatcac agacccttgc ctgggttggg ataacacaga agctcttgtc
     6781 agagaaattt acaaaacgtt aggagaataa gatggcattt attgaaaaag gtcaagaaat
     6841 cgatatggaa gtcatcaagg ctgaaaccca attgtctgcg gaagccttga gactcaagga
     6901 aagccgtgac agggaattgg cagatattat ttcaggggaa gatgaccgta ttctcttggt
     6961 gattggtcct tgctcttctg ataatgaaga ggcggtcttg gaatatgctc gccgtttatc
     7021 tgccttgcaa aagaaggtag cggataagat tttcatggtc atgcgcgtgt atactgctaa
     7081 gcctcgtacc aatggagacg gctataaagg attagttcac cagccagata cttctaaggc
     7141 tccaagcctg attaatggct tgcaggctgt gcgccagttg cactaccgcg tgattacaga
     7201 gactggtttg acaacggcag atgagatgct ttatccgtca aatctgatct tggtggatga
     7261 cttggtcagc taccatgccg ttggagctcg ttctgtggaa gaccaagagc accgctttgt
     7321 ggcttctggg attgatgcac cagtagggat gaaaaatcca acctcaggaa atttgggtgt
     7381 tatgtttaac gccatctatg ctgctcaaaa caagcaaacc ttcctttatc atgggcagga
     7441 agttgagaca tcaggtaatc ctttggccca tgttatcctc cgtggagcag tcaacgagta
     7501 tggcaattat atgccgaatt actactatga aaatctactc caagccattg aacgctatga
     7561 aaccatggga cttgaaaatc cttttatcct cattgacacc aaccatgata actcaggcaa
     7621 gcaatatatg gagcagattc gaattgttcg ccagaccttg cagaatcgtg attggaatga
     7681 gaaaattaaa aagacggttc gaggatttat gattgaatct tacctagcag atggtcgtca
     7741 aaaccaacca gagatctttg gttgctctat tactgaccct tgcctaggtt gggaaaatac
     7801 agaggccttg gtagaagaga tttatgttac cttgacaaaa taagtgaaaa ggatggagtt
     7861 ggggaatctc aactcctttt gatgagaatg atagttggac acggaattga catcgaagaa
     7921 ttggcttcga tagaaagcgc agttacacga catgaaggat ttgctaagcg tgtactgacc
     7981 gctcaggaaa tggagcgctt caccagtctc aaaggacgca ggcaaataga atatttagct
     8041 ggtcgctggt cggctaagga ggccttttcc aaggctatgg gaacgggcat tagcaagctc
     8101 ggttttcagg atttggaagt cttgaacaat gaacgtgggg cgccttattt tagtcaggca
     8161 ccattttcag gaaagatttg gctgtctatc agccacaccg atcagtttgt gacagccagt
     8221 gtcattttgg aggaaaatca tgaaagctag tccacataga ccaaccaagg ctctgattca
     8281 tctgggagct attcgacaaa atattcagca aatgggggct catatccctc aaggaacgct
     8341 caagttggct gtggtcaagg ccaatgctta tggtcatgga gctgttgccg ttgccaaggc
     8401 aattcaagat gatgttgatg gcttttgcgt ttccaatatc gatgaagcca ttgaactcag
     8461 acaagctgga ctcagcaagc caatcctcat tttaggagtt tctgaaatcg aagctgttgc
     8521 tctagctaaa gaatatgact tcaccttgac agtggctgga ctggagtgga ttcaagcact
     8581 cttagataag gaagtggacc taactggatt gacagtccac ctcaagattg attcagggat
     8641 gggacggatt ggttttagag aggcaagtga ggttgagcag gctcaagatt tgctccaaca
     8701 acacggtgtt tgtgttgaag gaatctttac ccactttgct actgctgatg aggaatcaga
     8761 tgactatttt aatgcccagt tagaacggtt taaaactatt ttagctagta tgaaggaagt
     8821 tccagagctg gttcatgcta gcaattctgc aacgactctt tggcatgtag agactatttt
     8881 caatgcggtt cgtatgggag atgccatgta tggcctaaat ccaagtggag cggtcttgga
     8941 tttgccttat gatttgatac cggccttgac cttggagtct gctctggttc atgtcaagac
     9001 agttccagct ggagcttgca tgggctatgg agcaacttat caagcggata gcgagcaagt
     9061 catcgcgacc gtgccaatcg ggtatgcaga tggatggaca agagacatgc aaaatttctc
     9121 tgtcttggta gatggccaag cttgcccaat tgtcggcagg gtttcgatgg accaaatcac
     9181 tattcgattg cctaagcctt acccactcgg aaccaaggta accttgattg gctccaatgg
     9241 ggataaggaa atcactgcaa ctcaggtagc gacctaccgc gtaaccatta actatgaggt
     9301 ggtttgcctc ctcagcgacc gtattccgag agaatattat tagaaaagaa aggagtggag
     9361 catgaatcta catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt cagcagaaaa
     9421 atacgccaaa ctaggaattg aaaacttgca agatctcttg ctctactttc ctttccgtta
     9481 tgaagacttc aaaaccaagc aggtgctgga gctggaagac ggtgagaagg cagttctttc
     9541 tggtcaggta gtgactcctg ctagtgtcca gtattatggt ttcaagcgca atcgcctgcg
     9601 ttttagtctc aagcagggag aggtcgtttt tgcggtgaat ttctttaacc agccctatct
     9661 ggctgataaa atagagttgg gagcaaccct tgctgtcttt ggaaaatggg accgcgctaa
     9721 ggctagtctg actgggatga aggttctggc tcaggtagaa gatgacctcc agcctgtcta
     9781 tcgtctggct cagggaatca gtcaggccag tctggttaag gtcatcaaga cggcttttga
     9841 tcagggactg gacctcttga tagaagaaaa tctgccccag tctttactag acaaatacaa
     9901 actcatgtcc cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt tggcagaata
     9961 caagcaggct cttcgccgta taaagtttga ggaactcttt tatttccaaa tgcagctgca
    10021 gatgctcaag tctgaaaata gagttcaggg aagtggtctg gttctgaatt ggtctcagga
    10081 aaaagtgaca gcagttaaag taagtcttcc ttttgccctg acccaagctc aggaaaagag
    10141 tttgcaggaa attttaactg atatgaagtc cgaccaccac atgaatcgtc tcctacaagg
    10201 ggatgtgggg agtggaaaaa cggtagtcgc tggcttggcc atgtttgcgg cagtgacagc
    10261 aggttatcag gctgccctaa tggtaccaac agaaatcctc gcagagcaac actttgagag
    10321 tttacagaac ctttttccca atttgaaact ggctttcttg ataggttcct tgaaagctgc
    10381 agaaaagaga gaggtcttgg agaccattgc caagggtgag gctgatttga ttatcggaac
    10441 tcacgctctg atacaagatg gggtggagta tgctcgtctt gctttgatta ttatcgatga
    10501 gcagcaccgt tttggtgtag ggcaaaggcg tattttacgg gaaaaaggtg acaatccaga
    10561 tgtcctcatg atgacggcga ctcccattcc acggacgctt gccatcacag cctttggaga
    10621 tatggatgtt tccattatcg accagatgcc agcaggtcgg aagcctattg tgacgcgctg
    10681 gatcaaacat gagcaactac ctcaggtctt gacttggtta gagggggaaa ttcaaaaagg
    10741 ttcccaagcc tatgtcatct ctcctttgat tgaagaatca gaagctctag atttgaaaaa
    10801 tgccattgcc ttatcagagg agttgacgac tcattttgca ggcaaggcag aggtggctct
    10861 tctacatggt aggatgaaga gtgacgaaaa agaccagatc atgcaggatt tcaaggagag
    10921 aaagacggat attctggttt cgacgacggt tattgaggtt ggggtcaacg ttcccaatgc
    10981 gactgtcatg attatcatgg atgccgatcg cttcggtctc agtcaacttc accagcttag
    11041 aggtcgtgtc ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcta atcccaagac
    11101 ggattctggg aaagaccgca tgcgtatcat gacagaaacg accaatggat ttgtccttgc
    11161 ggaggaagat ttgaaaatgc gtggttctgg tgagattttt ggaaccagac agtcaggact
    11221 tccagagttc caagtggctg atattatcga agattttccg attttagaag aagcaagaaa
    11281 ggttgctagc tacattagtt ctatagaagc ttggcaagaa gatccagagt ggcgcatgat
    11341 tgcccttcat ctggaaaaga aagaacatct ggattaagct ttctctaagg aaaacttaag
    11401 ctagttttta ggtttggctc ttatactaaa gtcatcaaaa agaaacgagg actctcatat
    11461 gacagtaacg attaaagtaa attaccaaac cactttccaa aagaaggaag caaaaaacta
    11521 gtataaacag aagagagagc gaaatgctct tttttcgttt ctaaaactac tttcagccca
    11581 tcatcctaaa agtaaagaat ctaaattcac tttctattta cccttctttc ttgcattgat
    11641 tacatagata tgctacagtt gtggtaacga ttacaaaata aaaggagcat gctatgaaaa
    11701 atccagcttt gctagaagaa attaagacct atagaggaag ggatgaggtt ccggaagact
    11761 ttgatgcttt ctgggatggg gaagtgaaaa atgtttccac acttccatcc taccacttgg
    11821 aggaaagaga tttccacatt cctcaagtca agtgctatga gttaacattt gaaggaagca
    11881 aggaaggaaa ggtctatgca cgcattgttc ttccaaagag tgaggagaag gttccattaa
    11941 tcttccattt tcatggttat atgggacgtg gctgggactg ggccgacatg ctgggcttca
    12001 ccgtagctgg ttacggtgtt gtttccatgg atgtgcgggg ccagtcaggt tactcacaag
    12061 acggcttgcg ttctccttta ggaaataccg tgaaggggca tattatccgt ggtgctatgg
    12121 aaggtcggga ccacctcttt tataaggatg tttatctgga tatttaccag ttggtcgaaa
    12181 ttgttgctag tctgtctcag gttgatgaga agcgtctttc tagctatggt gcctcacaag
    12241 gaggggctct agctctagtt gcagcagcgc tcaatcctcg aattcagaaa acagttgcca
    12301 tttatccctt cttgtcagac ttcagacggg tgattgagat tggtaatact agcgaggctt
    12361 acgacgaact tttccgttat ttcaagtttc acgacccctt ccatgaaaca gaggaggaaa
    12421 tcatggcgac ccttgcctat atcgatgtca aaaatcttgc ccatcgtatc caaggtgagg
    12481 ttaagatgat tacgggcttg gacgacgatg tttgctatcc cattacccag tttgcgattt
    12541 ataatcgtct gacctgcgat aaaacctatc gcatcatgcc tgagtatgct cacgaagcca
    12601 tgaatgtatt tgtcaatgac caagtctaca actggctctg tggaagtgag attcctttta
    12661 aatatctaaa ataaggagtc gactctaagc acaaaatctt aaaaattaca aacacgcata
    12721 gtatcagggg attaaaaaaa cttgatacta tgcgttttat catggaaata tagtaaaatg
    12781 aaacaagaat aggacaaaac gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
    12841 gcaaatgtgt actattctag tttcaatcta ttatatttat agaatttttt gttgctagat
    12901 ttgtcaaatt gcttaaaata atttttttca gaaagcaaaa gccgatacct atcgagtagg
    12961 gtagttcttg ctatcgtcag gcttgtctgt aggtgttaat acttttcaaa aatctcttta
    13021 aaccacgtca gcttcgcctt gccgtatata tgtt
//
LOCUS       DAVMHG010000046        12835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMHG010000046 DAVMHG010000000
VERSION     DAVMHG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.046"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..994)
                     /locus_tag="VYR08_001077"
                     /pseudo
     CDS             complement(1..994)
                     /locus_tag="VYR08_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            1150..1596
                     /locus_tag="VYR08_001078"
                     /pseudo
     CDS             1150..1596
                     /locus_tag="VYR08_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1722..1988
                     /locus_tag="VYR08_001079"
     CDS             1722..1988
                     /locus_tag="VYR08_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3466617.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            2249..4498
                     /gene="metE"
                     /locus_tag="VYR08_001080"
     CDS             2249..4498
                     /gene="metE"
                     /locus_tag="VYR08_001080"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV3466618.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            4562..5428
                     /gene="metF"
                     /locus_tag="VYR08_001081"
     CDS             4562..5428
                     /gene="metF"
                     /locus_tag="VYR08_001081"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV3466619.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            6113..8326
                     /gene="pnp"
                     /locus_tag="VYR08_001082"
     CDS             6113..8326
                     /gene="pnp"
                     /locus_tag="VYR08_001082"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV3466620.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            8342..8959
                     /gene="cysE"
                     /locus_tag="VYR08_001083"
     CDS             8342..8959
                     /gene="cysE"
                     /locus_tag="VYR08_001083"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV3466621.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            8971..9855
                     /locus_tag="VYR08_001084"
     CDS             8971..9855
                     /locus_tag="VYR08_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466622.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            9942..11285
                     /locus_tag="VYR08_001085"
     CDS             9942..11285
                     /locus_tag="VYR08_001085"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV3466623.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            11278..11664
                     /locus_tag="VYR08_001086"
     CDS             11278..11664
                     /locus_tag="VYR08_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV3466624.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            11668..12552
                     /locus_tag="VYR08_001087"
     CDS             11668..12552
                     /locus_tag="VYR08_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV3466625.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         3844 a         2477 c         2890 g         3624 t
ORIGIN      
        1 gtgatttctg taaaaataca tctatgatca gagttttgaa aacttacaat gtctaaatct
       61 ttaacatagt agtggttttt aggcaataaa atatgatcta ttgttgcatt aaaaagtttt
      121 ggactttggc tattccaagt tcctctttca aaaggtggca gtacatttaa tgcgtcccta
      181 tgagagctta tttttgcaag tgctccatga gcatagttgc attaaaatca cctgcaataa
      241 tagcctttgg atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga
      301 tttccattaa acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt
      361 gttttctcga atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg
      421 tatagaaacc ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag
      481 gtggagaagt gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt
      541 tgattctctg gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca
      601 tatcggcgtc aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga
      661 cgttccaagt tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat
      721 ctgttccgaa taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta
      781 tttgtttatt tttcttgtaa aaagataggg aaaaaaatac gaaacttaaa atagcagcca
      841 tagttgcagt agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc
      901 aaatgttaag attagaagat gggaaaaaag aataagttat aatagggata gaaaagagaa
      961 tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg
     1021 ttgctcggag ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc
     1081 ctcatagatt aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa
     1141 ggcacagaaa tgcaagacaa ttatactaca aaaggcaaac atttgacaat cgatagccgt
     1201 cgcttaatcg aacgatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt
     1261 ggaaaagctc ctcaaactat ccatactgaa atcaagtatg ggacagtccg acaatgtctt
     1321 ggaaaagggc gcttcaaaga gatttattct gccgactacg ctcaacagtc ttatgaaaat
     1381 aatcgcaagc actcggtcaa gaaatcaagc ttgactaaga aactaaagga aaagattctc
     1441 cactatcata accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg
     1501 ggaatttcaa ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat
     1561 ctgctttatc ctagaaaagg aaaagcgctt aagaaatagg ctagcaccaa ctttaaaccc
     1621 gctggtcaat ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaaa
     1681 aaagagggtt aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg
     1741 agctctttag caaggaggaa atccttgaaa gtgatatgcg agtggctatc atgagcgagt
     1801 tgattgaggc taggtatgaa caaggaatca gtcagaaaaa gctggaagaa gtcagtggaa
     1861 taagccagcc tgtcatagct aggatggaga caggagagag cagtcttcag ttggatacgg
     1921 tcttaaaagt tctaaccagt ctaggaaaga cactagcagt cgttccactt gaacagggga
     1981 aaagttgact agaatcagtt atagtctttt ctaataacag gtcatagtca cttataagaa
     2041 ttactaataa catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa
     2101 ggatttgaga cgtttgcttc aagtcttttt cttttgtccg aaacagatat atagtttcaa
     2161 actatatcaa accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt
     2221 ttcaacaaca aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt
     2281 gggcgaattc cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga
     2341 agaagaactt ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga
     2401 aaaaggcatc actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc
     2461 ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga
     2521 gcgctacttc gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc
     2581 aatgaagaaa tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac
     2641 tcaagtaaaa ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg
     2701 attgaacact cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga
     2761 agaaggcgtg aaagcagacg acttcgtaga tagcttagtg gttgcttacc aagaagtttt
     2821 tgctaaattg gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa
     2881 agacttgaca gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga
     2941 caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc
     3001 tgaccttgta aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac
     3061 tcttgaactc gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa
     3121 tggtaaaaac atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc
     3181 agctgaaaac atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc
     3241 taatgaagaa tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga
     3301 tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa
     3361 caaagaactc tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc
     3421 tggcttgaca gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca
     3481 agaagaagct ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac
     3541 aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta
     3601 cgatgctttc cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt
     3661 tgatgtcctt gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa
     3721 cttgtcagga tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt
     3781 aaaaccacca atcatctggg gtgatgtcac tcgtcttaac cctatcactg ttaaatggtc
     3841 tagctatgca caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac
     3901 catccttaac tggtcattcc cacgtgaaga catctctatc aaggattcaa ctcttcaaat
     3961 cgcccttgct atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca
     4021 aatcgacgag gctgctcttc gtgaaaaatt gccactccgc cgtagcgact ggtacgaaga
     4081 ctaccttgac tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac
     4141 acaaatccac actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa
     4201 catggatgca gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga
     4261 actcaaagcg aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc
     4321 tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc
     4381 aagcaagaaa gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac
     4441 aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga
     4501 ttggatttct ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca
     4561 tatgtcacgc caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg
     4621 taatggtaac attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag
     4681 tgtaactgcc agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt
     4741 tatccaaaat gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa
     4801 ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc
     4861 tcttcgtggg gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga
     4921 cttgattgag tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc
     4981 agaagggcat ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt
     5041 ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt gataatgagc gcttctatga
     5101 ttttcaggac aagtgtattt tggctgggat tgatgttccc attcatgcag gaattatgcc
     5161 aattctgaat cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc
     5221 acgcaaattt aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc
     5281 aggacttgcc tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt
     5341 gcatctctat acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc
     5401 cttgtttaat caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga
     5461 atggttcctt tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc
     5521 tgtacgtact tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga
     5581 tttaacgaca cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta
     5641 gctaatcgcc cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct
     5701 aatgatgtat cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct
     5761 aggagcagtc gctccgtcgt tcgatttcct attttccttt gagttgcttg acggcttggt
     5821 atcttgatga cttgaatttt ggtctcattt cttaggaaaa aagataagct tcctagcact
     5881 cgtcgagtgc gacgtcacct tcctattttt ctacagaaat gttcggcaag ccgaaccgtc
     5941 caaaatatct tgtgtaattg aacacggccg aaaagctgtg taaaaaagat aaactgtctt
     6001 gtcttcatcg aagacttcgt cagtttccta tttttacttt gcttttgacg tccttggtat
     6061 cttgatcttt gtaggcaagg cgtataattt catcaatcca aaggggatta aaatggcaaa
     6121 acaagtgttt caaacgactt ttgcgggtcg tgagttaatt gtagaaactg gtcaggttgc
     6181 taagcaagca aatggctctg ttgtcgtacg ttacggtgag tcaactgtct tgactgctgc
     6241 cgttatgtct aagaaaatgg caactgggga tttcttccca ctccaagtca actacgaaga
     6301 aaaaatgtat gcggctggga agtttcctgg tggctttatg aaacgtgaag gacgtccttc
     6361 aacagatgcg accttgacag cgcgtttgat tgaccgtccg attcgtccta tgtttgcgga
     6421 aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt tcttatgatg aaaatgcatc
     6481 tgcaccaatg gctgctatgt ttggttcatc tttggcactg tctatttcag atattccatt
     6541 tgacggacca attgctgggg tacaagtggg atatgtagat ggccaaatca tcatcaaccc
     6601 aagtcaagaa caagcagagc aatctcttct tgaattgaca gtagctggaa ccaagcacgc
     6661 tatcaacatg gtagagtctg gtgccaaaga attgtcagaa gaaatcatgt tggaagcgct
     6721 tcttaaaggg cacgaagctg tcaaagaatt gattgccttc caagaagaaa tcgttgctgc
     6781 tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg gatgctgaat tgcaagctga
     6841 aatcattgca gcctacaaca gtgacctcca aaaggcagtt caagtagaag agaaattggc
     6901 ccgtgaagct gcaactcaag cagtgaaaga ccaagtgact gccgtttacg aagaaaaata
     6961 tgcgaaccac gaagaatttg accgtattat gcgtgatgtg gctgaaatct tggaacaaat
     7021 ggaacacgca gaagtgcgac gtttaattac agaagacaag gtgcgtcctg atggtcgtaa
     7081 ggtcgatgaa atccgtcctt tggatgcggt tgttgacttc cttcctcgtg tacatggttc
     7141 aggtctcttt actcgtgggc aaactcaagc tctttcagtc ttgaccttgg ctccgatggg
     7201 agaaactcaa atcattgatg gtttggatcc agagtacaag aaacgcttta tgcaccacta
     7261 taacttccct caatattctg taggggaaac aggtcgttac ggtgcgccag gtcgtcgtga
     7321 aatcggtcac ggtgcccttg gtgagcgtgc tcttgctcaa gtcttgccaa gcttggaaga
     7381 attcccatac gctatccgtc tagtagcaga agttttggaa tcaaacggtt cttcatctca
     7441 agcttctatc tgtgcgggaa ctcttgccct tatgactggt ggtgtgccaa tcaaggcgcc
     7501 agtagctggt attgctatgg gacttatctc agatggaaat aactacacag tattgacaga
     7561 tatccaaggt ttggaagatc actttggaga tatggacttc aaggttgcag gtactcgtga
     7621 tgggattaca gcccttcaaa tggatatcaa gattcaaggg attactgcag aaatcttgac
     7681 ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc cttgatgtca ttgaagcaac
     7741 cattccagaa gttcgtccag aattggctcc aactgctccg aaaattgata cgatcaagat
     7801 tgatgtggac aagattaaga ttgtcatcgg taagggtgga gaaaccatcg acaagattat
     7861 cgctgaaaca ggtgttaaga ttgatataga cgaagaagga aatgtgtcta tctactctag
     7921 tgaccaagat gctattaacc gtgccaaaga aattattgct ggtttggttc gtgaagccaa
     7981 agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag aaatttggtg cctttgttaa
     8041 cctctttgat aagacagatg cccttgttca tatctctgag atggcttgga ctcgtaccaa
     8101 tcgtgtagag gatttggtag aaatcgggga tgaagttgat gttaaggtta tcaagattga
     8161 tgaaaaaggc cgtatcgatg cctctatgaa ggctcttcta cctcgtccgc caaaacctga
     8221 gcatgatgaa aaaggtgaaa agtctgagcg ccctcaccgc ccacgtcatc acaaggatca
     8281 caaacctaag aaagaattta cagaaacacc aaaagattca gagtaagaaa aggagaaatg
     8341 tatggggtgg tggcacgaaa ccattgatat cgtaaaagaa aatgatccag cggcccgcac
     8401 cactttggag gttttgctga cttatccagg tgtcaaggcc ttggcggccc accgtctctc
     8461 gcattttctc tggaagtacg acttcaaact cttagctcgt atgcacagtc agttttggcg
     8521 cttttggact cagattgaga ttcatccagg agcccagatt gattcaggtg tctttattga
     8581 ccatggttct ggtctggtga ttggagagac agcgattgtt gaaaaaggcg ttcttctcta
     8641 tcacggagtg actctcgggg gaacagggaa agactgtggc aaacgccatc cgactgtacg
     8701 aaagggagcc ctcatatcag cccatgccca agttatcggg cctgtggaaa ttggtgaaaa
     8761 tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta cctagtgatg tgacggttgt
     8821 cggtattccg gccaagattg tccgtcttca tggtaagaaa gatgagcctg ttattcacga
     8881 agtcgaagaa aaacgggagt attatgtcaa taaactcgag caggctaaag atgccagtca
     8941 cagatcgtct ggtttgtaga ggatacctat atgattcaag caagaaacaa gttaagccaa
     9001 gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc aaaactatga cggtacctat
     9061 cgcgatccct atctctctaa catgcttaat tttgacccaa acatgcccgc ctttttcctt
     9121 tattatgaaa aaggcaaact tgttggttta ttaactgtct atgcagatga ccaagatgta
     9181 gaagtgacga tattggttca tccaggtcat cgccgtcagg ggattgcgcg tgcattgttt
     9241 actagttttg agagagaaac agcttctttc cccattcggt cagtcacttt tcagacagaa
     9301 cgtatttttt tagagaatca tcctgacttt gccagtaact ggggactaat cgaggatgaa
     9361 gagacagaaa cttggttagg taaggataga agaccttatc cgttagcaaa tgtttcccat
     9421 cttgaagttt tgttagcaga tagttcgtat caggatcaaa ttagtcaatt aaaatttcag
     9481 gcattttcag aggaacatga atcgagagaa gttgtggata gatatgtcgc tgaagctctg
     9541 aaggatccag aaagtcgctt atatattttg ttaaaagacg gtcaggttat tgggacttgc
     9601 acggtagatt tatcgactaa tacgaattac ttctacggtt tagcaatatc ggaacctgaa
     9661 cgtgggaaag gctatggaag ctacttagca aaatccctcg tcaaccaact aattgagcaa
     9721 aatgacaagg aatttcagat tgcagtggaa gatagcaatg taggtgccaa acgtttgtat
     9781 gaaaaaattg gctttgtcaa acagacacag gtggtttatc tgaatgagaa aggagcaagg
     9841 gattccgaag tgtagagaca ttcggactga aatttacttg agctcttagt ttagtgatga
     9901 aactaacagt tcttggattt tagctttcct gattatgatt tatgattaaa atctatgaca
     9961 ccatgtctcg tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg
    10021 tttgtgggcc aaccgtgtac aactatattc acgtgggaaa cgcccgttcg acggtagctt
    10081 ttgatacgat tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt
    10141 ttacagatgt ggatgataag attatcaacc gtgccaggga agaaggcatc acacctcagg
    10201 aggttgcgga taagtacatc gctgcctttc gtgaggatgt gacggcctta ggcgtgaaac
    10261 ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag
    10321 acttgatcga gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa
    10381 aatcccacaa ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt
    10441 caggtcgtac cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga
    10501 aatcttccaa accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt
    10561 ggcatattga gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg
    10621 gtggtggagc tgacctagag tttccacacc acaccaatga aattgcccag tctgaagcta
    10681 aaacaggcaa ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg
    10741 tcaaaatgtc taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg
    10801 atgggcaagt acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca
    10861 cggaaaaggc agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac
    10921 aaccatttac aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag
    10981 cagctatgga tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca
    11041 aatggatcaa ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag
    11101 aaatttttgg aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc
    11161 aaaaacgcca agaggcgcgt gccaatcgtg actttgcgac agcagaccaa atccgtgacc
    11221 aattggttac tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg
    11281 attgatgtca atctcattaa cgggattgcg ctagcctttg agggggatgc ggtgtattct
    11341 atgtatattc gccgtcacct catcctcaaa ggtatgacca aacccaataa actccatcaa
    11401 gaagcaacta agtacgtgtc agccaaggct caggctcgcc tgattgctct catgttggag
    11461 gagcaggtcc taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagc
    11521 cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagcg
    11581 gttatgggct atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg
    11641 tgcatccaaa aagtggaggg ctagaacatg agggcaaaag aactacaaga ctggtttcct
    11701 gaggctcgga tttcagacca accagtagag aaagagggct atctcacgct ccctttagct
    11761 tctcagcagt ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt
    11821 gcccttttga cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt
    11881 gagggcaagg gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt
    11941 tcttattttc agcaggaaaa tctggcttct tggctagata tgatgcggac tctttttccg
    12001 aattgtcaga cagtgctaca ggtcggagct caggattatg ttttcgtgct tcaacaagac
    12061 aaatacactt ctgtaagaga tattttaagt gatacgattg aagcggttga gtatgacttt
    12121 ggacttcgtc tttctatcat gttgggtcag gtttggtctc agacgggaca tcaagcccta
    12181 tcagacttaa tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa
    12241 ggtgttcata ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg
    12301 aagccaatca aggaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc
    12361 attctctctc tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg
    12421 caccgcaatt ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg
    12481 aaagagttga ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata
    12541 gttggtttgt aaagaagtta atatttggag aagaggattg ccatcttctc catttttatg
    12601 tgcagagtta tagtggcttg atgctgggaa agtacactgt gactgctaaa acattcctag
    12661 aagctgcttt gatttcccta atctatttat gcaaatttta tgttatttta ctataacagt
    12721 tgttgctaag ccaaataata gtggggaagt tcatttagac gtaagcattg aagataatca
    12781 gggaggtagt gggtataatt tcagttctgt ttcaagtagc tcacaaacag ctaaa
//
LOCUS       DAVMHG010000047        12568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMHG010000047 DAVMHG010000000
VERSION     DAVMHG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.047"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(73..489)
                     /locus_tag="VYR08_001088"
     CDS             complement(73..489)
                     /locus_tag="VYR08_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466626.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(501..1196)
                     /locus_tag="VYR08_001089"
     CDS             complement(501..1196)
                     /locus_tag="VYR08_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466627.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1390..3891)
                     /locus_tag="VYR08_001090"
     CDS             complement(1390..3891)
                     /locus_tag="VYR08_001090"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV3466628.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4346..5434)
                     /locus_tag="VYR08_001091"
     CDS             complement(4346..5434)
                     /locus_tag="VYR08_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV3466629.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5452..6120)
                     /locus_tag="VYR08_001092"
     CDS             complement(5452..6120)
                     /locus_tag="VYR08_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV3466630.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SSSI"
     gene            complement(6139..8577)
                     /locus_tag="VYR08_001093"
     CDS             complement(6139..8577)
                     /locus_tag="VYR08_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV3466631.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8728..10026)
                     /locus_tag="VYR08_001094"
     CDS             complement(8728..10026)
                     /locus_tag="VYR08_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3466632.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(10043..10351)
                     /locus_tag="VYR08_001095"
     CDS             complement(10043..10351)
                     /locus_tag="VYR08_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3466633.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10397..10717)
                     /locus_tag="VYR08_001096"
     CDS             complement(10397..10717)
                     /locus_tag="VYR08_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3466634.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(10912..11892)
                     /locus_tag="VYR08_001097"
     CDS             complement(10912..11892)
                     /locus_tag="VYR08_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV3466635.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKESSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(11905..>12568)
                     /locus_tag="VYR08_001098"
     CDS             complement(11905..>12568)
                     /locus_tag="VYR08_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3466636.1"
                     /translation="SKVTIRKDLDKLESKGLLHREHGYAVLNSGDDLNVRLSINYEIK
                     RKIVQEAVKLVSDNETIMIESGSTCALLAEEICKQKRNVTIVTNSFFIANFVRAYDSC
                     RIIVLGGEFQKDSQVTVGPLLKEMIQTFHVRQAFVGTDGYDKEMGFTGKDLMRSEVVQ
                     YISAASDKVIVLTDSSKFDKRGTVRRFALSQVYEVITDEKLSKQNMATLENAGIMVKV
                     VS"
BASE COUNT         3884 a         2707 c         1967 g         4010 t
ORIGIN      
        1 aggaggtctt tatttaatga ctatataata ctagaccatc cttaaatttt gcttaagaaa
       61 aaacaaattt tgttattttt tacgattcat ccaagtcatc cctatacaat tataggtgga
      121 taagatttca aagcccatag aacgatagaa gcccaaggtt ctttctgtct cttctgtcac
      181 cagctggact tgataggcat ctttgtaatc ctctaaagcc tctttcatca aggcactacc
      241 aattccttga cgctgataga ttggtaaaac gattaaatcc tgaaccaata ctgatgagaa
      301 tccatctcca accaaacgaa tcaagcccac cacagcatca ccatcaagtg ccagataaat
      361 tactaatgaa tgagataagg cctgctccag catctctggt tgatgggtat aatttgtcca
      421 accgacagcc tgatagagat gtaaaacatc ctctagcttg acaatttctt gctttttaat
      481 agtaatcatc tcaacacctc ttaaagttct ctcaagctct tgtactgctg tccattttta
      541 tcaaaatttt caggacgcaa ccatgtttcc aaacgatctt tgacttgagg ccagtcctta
      601 tcaatcatag acaaccaatc cgtatctctc gtacgtccct tataaaccac tgcctgacgg
      661 aaggttcctt cataaataaa gctcaaacgc tccgcagctc gtctggatgg cagatttaag
      721 gcatcgcatt tccactcgta gcgacgatag tttagctcct caaaaacata gcgagctagg
      781 agatactgag cttcagttcc tatccgtgta ccccttagct ctggagaaaa agtgactgct
      841 ccgacttcta ttactcggtt attctggtca atgcgcatga gagaaaaaga tcccaaagcc
      901 ttaccagtcg ccttgtctat gattgcatag taaaaacggt ccttacgagc caacatctga
      961 tgtaaaaggc taaccagctc ccccatatct gctactggct cctgaaaaag gtaggtccac
     1021 atctcccgag gagtatccgg gccataaaca gctagcaaat cctccgcatg cttttccact
     1081 gaaagagcct ctatcctagc ataacgccct tctaagaaat caatagaagg cagttcacct
     1141 ggtgtataac cttccattga ctccccaatc atttgaccat attcatttac tggcatagct
     1201 ttctcctttt ttcacttttc aaactttata gttgcccata gtataccata ctttcataag
     1261 aagattcaaa aaatcctcca gattctactc tgaaggattc ctaccttatt tcacaacgat
     1321 attaacgagt ttagttggct caaacagttt gggaaactgt ttgaggttgg aaatcagcaa
     1381 ataaattggt tattttacaa caatattaac caatttattt ggtacactaa tcactttcac
     1441 gatttcctta ccgtcaattt ctgctttgac tttttcatcc gctagagcaa tttcttgcaa
     1501 ttcttcacgt gataggtctt tagcgaccat gagtttggca cggactttac ctttgatttg
     1561 gacaacgatt tcgatttcgt cttcaaccag tttactttcg tcccaagttg gccaagctac
     1621 ataagagatt gactcacctg tttctgcgac tgtttgccag agttcttctg ccaagtgagg
     1681 tgcaaatggt gcaatcaatt gaataaagcc tttggcatag tcaacataaa gcttatcttc
     1741 cttgttagca gcattgacaa agaccataag ttgggcaata gctgtgttga atttgagaga
     1801 ctcaatttgc tcagtaacag ctttgactgt ttcgttgtaa gccttgtcaa gagcaccatt
     1861 gttttccgca aggatttctt tacttgtaat caaacggtaa actcggtcaa ggaacttacg
     1921 gcttccttcc aaaccttctt ctgaccaagc aatcgaagca tcgagtggtc ccataaacat
     1981 ttcataaaca cgaagggtat cggcaccgta ttgttccacc acatcatctg ggttaacaac
     2041 gttcttgagc gatttagaca tcttggctgg cgcttgctcc aactcttccc ctgtttctat
     2101 atgaaagaag gaaccatcac gtttttcaac cttgtcggtt gccacaagag caccacggtg
     2161 gtcacggtag cttgttccca aaatcatccc ttggttaaag agtttttgga atggttcctt
     2221 agtcggaaca acaccgaggt catagaggaa tttatgccag aaacgagcat aaagcaagtg
     2281 aagtacagca tgttccgcac cacccacgta gatatctact ggcaaccatt gtttgaggag
     2341 gtcctcatca gccaatttct cagtattgtg cgggtcaata tagcggaggt agtaccagct
     2401 tgaaccagcc cattgtggca tggtgttagt ttcacgacga cctttgacac catcttcacg
     2461 agtcacttca agccaatctg tcaagttagc tagtggactt tcaccagtac ctgaaggacg
     2521 gatatccttg gttacaggca agacaagcgg caattcagtt tcaggaacag ctgttgaagt
     2581 tccatcttcc caatgaatga ttggaattgg ctcaccccag taacgttgac ggctaaagag
     2641 ccagtcgcgg aggcggtagg taaccttctc ctgaccacag cctttttctt ccaagcaagc
     2701 cacaatcttg gcaatagcgt cttctttgtt caatccatct aggaagtctg aattgacatg
     2761 caggccatcc tctgtgtagg cagcttcttc gacatttcca ccttcaagta cttcgacgat
     2821 tggaaggtca aattgtttgg caaattccca gtcacgttgg tcgtgggcag gcacagccat
     2881 aaccgcacct gttccataac tagcaagaac atagtccgca atccagattg gcatttcctt
     2941 accattgaca gggttgatgg cataggcacc tgtccaaaca ccagtttttt ccttagcaag
     3001 gtctgtacga accaagtcag acttaaggct ggcttggtgt ttatagtctg ctacagcttc
     3061 tgcttgctct gaacttgtga tagcgtctac taattcatgt tcaggagcca agacagtgaa
     3121 agtcgcaccg aaaagtgtgt ccggacgagt agtaaagact gtaaattcct tgtctgttcc
     3181 ttttactttg aaagttacat tggcaccagt tgatttacca atccagttgc gttgcatatc
     3241 cttgatagac tctgaccaat ctagttcatc taagtcattg agcaagcgct ctgcgtaagc
     3301 cgtgattttg agcatccatt ggcgcattgg tttgcggaca actggatagc ctccacgctc
     3361 agaagttccg tcaggaagca cttcttcatt ggcaatggca gttcccaatt cctcaaccca
     3421 gtttactggc acttcagctt cataggccaa gcctttttcg taaagcttgg tgaaaatcca
     3481 ttgagtccac ttgtagtagt ttggatctgt tgtgttgact tcacgatccc agtcataaga
     3541 aaatccaagc gcattgattt gacgtttgaa gttggcaatg ttttccgctg taaattctgc
     3601 tgggtcatta ccagtatcca tagcgtattg ctctgcaggc aaaccaaaag catcccaacc
     3661 cattgggtga agaacattgt agccttgcgc acgtttgtaa cgactgagga tatcggttgc
     3721 agtataacct tctgggtgtc ctacgtgcag accagctcca gacggataag ggaacatatc
     3781 aagcgcataa aacttaggtt ttgatgcatc tgttcctgtc ttaaatgtat gatgttctgc
     3841 ccagtagccc tgccacttag gctcaatttc tttatgatta taaaaactca tggtctctct
     3901 ccaattttgt gatgttacta ttataccatt tttgagggaa tttgaaatgt gtttaaagga
     3961 aaaactgtat actgtatctc attaattttg atgactatga attgctctat ctcccacttt
     4021 ttatcccatt gttatgcagt aaatcaagga gtaagtcaag aagcttatct cgtatagcac
     4081 aaaatactta ttttagaccg aagataaaac tggaatgcta attaatgtat tcacaataaa
     4141 tagccgacaa cctatgtcgc tttttgattt atgaagaacg tatagtgcac tgaatctgaa
     4201 atagtacgaa acaattgcta aaacattgtt agaaattaat tttactttcc caatcgattt
     4261 gttctcatct tatttcaatc tgctatagaa ttcaatacga ggatcaataa aagactacca
     4321 tttggaaaac agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa
     4381 tagcttgagc aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct
     4441 ccatagttgc ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca
     4501 tttcttttag agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt
     4561 cttctttagg tctattttcc aataatagtt gtactaaagt tccataagct actttttcac
     4621 catgtgttaa atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg
     4681 ccgcagctaa tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa
     4741 tattttctaa tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat
     4801 ctgcaaacag cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt
     4861 gtcccaacat agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta
     4921 aaccatctgc aataccagac gctaataaac gcttaggggc ttgtgaaata acttttgtat
     4981 caaccaaaac taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat
     5041 ctgtataaat aacagataaa gcagatacag gggcgtcggt cgatgcaatt gttggagcaa
     5101 taataacagg tttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc
     5161 caccaagacc gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga
     5221 tttcattgtc agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat
     5281 gtagataatc ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa
     5341 tcgggtaatt tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct
     5401 gaatatatct agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga
     5461 actactattt tgagtaacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga
     5521 tggcatggcg aaagctgatt caaaaacatc tgctcctgct gtaacagcat gcgcacctgc
     5581 agctaaagca ttatttactt gtgctacatt tttaaaggat gcagctaaaa tcttactagg
     5641 agagttctgt ctatcaatag caagagctaa ttgacgaatg acagaatttg aatcaatgtt
     5701 cagattttcc attctattat aatatggagc taggtaatcc gctcctgctt cgatagctaa
     5761 taatccctga ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag
     5821 cacctttatt gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc
     5881 tgcttgtctt cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac
     5941 ctgaacatga atagagggtg tagagccaat caattctctt acatctttga ttcgttcaaa
     6001 aaaattaata gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg
     6061 caaaatttca gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat
     6121 aaaaagaacc tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt
     6181 tgccttagaa agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct
     6241 gtgtttttca ggatgtttct gagcgtcaat cagcgtctct ctggaaacaa cattgtattg
     6301 aatatggtac ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt
     6361 atcttcttct ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac
     6421 gatttcatct gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc
     6481 catattatgt gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg
     6541 agttgctaat gttccacgtc cctgccctac gttggctgag atagaagatg ttcctgaata
     6601 acgaattcct ccaattggac ctcttccata acgtgtatta ggatatttag caatttcatc
     6661 aacataaatg tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata
     6721 cttaggtgca tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc
     6781 tgtttccagt gcatgccaaa gctgacttgg gcttatacgt tcttcctcaa acaccaattt
     6841 tttaattgca gctaatgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata
     6901 atcatatact gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa
     6961 tgctgaacat agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac
     7021 aataacactc attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga
     7081 aaagttcttc atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg
     7141 atcaattcca tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc
     7201 tgtgcaacga tagccccatt tccctggaac tgccgtttca acacatccaa tggcactgta
     7261 atcataagca tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc
     7321 attattaaat gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa
     7381 acgagcatct aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg
     7441 tgcaactgat tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt
     7501 ctgtccacca attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga
     7561 ttgactgcga accttattaa ttgtaattgt cttaatccaa agatttgtca gacgttcaac
     7621 aatgctatct tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata
     7681 ttgatcaaaa cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg
     7741 aataaaccaa actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct
     7801 agagcaaata tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc
     7861 taaactttta gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat
     7921 aaatatagag tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc
     7981 tacttttgct ttacgagccc gctcttcaaa acctcttaaa ccaaattgca aaagtttctg
     8041 atagttaact gctaagtgag catctccaga attcatctta ccttccatac cgaagaatcc
     8101 tgtttccata taaactgaca cttcttcagg taataaggca ccagctctag cacgtaaatt
     8161 attattttcc caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata
     8221 gaaaacatct ccatcacgct tttcaaaaag atccaactca ttaagaacaa attctagcgt
     8281 atattccgga aaaataggag catctttatt ggaagaagct tgatttcccg caatcataga
     8341 ttcttcttca atatagatag acatattttc caaaatttct ttcagcatat atgcacgttt
     8401 taggacatta ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc
     8461 agcatcaata taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt
     8521 aaggctgcca aaatattctg tcttgattgt tgttcttgct acttctgtat ttaccatcgt
     8581 aagcaccttt ctttcgaaaa tatttttatt tctttcgaaa atataatacc gatttttata
     8641 gaaattttca agcttttaac tagaaaaatt ttaactctct aatttttagc acaaatgtgt
     8701 gagtttaaca gtaacacaca aatacctcta agattgtttg atttcatttt ggtaagctaa
     8761 acgatcttgt atcttaaaga atggaaagta aaccactgta gatatcacca atactaataa
     8821 ctgagtcaca acaccttgcc atccacccac caaaaatcct gatagaatag ctggtgtact
     8881 ccaaggcaat gttacccctg agaatggctg catgaaacct gttgcaatag ctccatatac
     8941 tatcacagct gcaagtacag gaacaagaat gaaaggtaca aacataactg gattcatgac
     9001 aatcggaaat ccaaatacaa ctggctcatt tacgttaaat attgctggaa aagctgcaac
     9061 ttttcctaag gcttggtatt gttttgattt tgctgcaaaa agcatggcaa ctacaagacc
     9121 aaacgtaatc cctgaacctg atagaattaa aaatgaatct aaaaattgtt gagtaacaat
     9181 atgtgcacca ttttctaatg atagattacc agaggctaac atagctttat tagcatcaag
     9241 attagataaa agcagagctg tcactactcc atttactacc gattgcccat gaacaccaaa
     9301 ccaccacaaa aatgatataa agaatgcaat tccaatagca ccatacaaag atccagttaa
     9361 accttgcaac ggaacttgaa tagcagaata aatcatttct atgaatgttc cgccattagt
     9421 caatgacttc gctaaaatat atacaatcat agaagataag aaaattacaa atgctggaat
     9481 cattgcttca aactgtttgg caatagcttg tggaacttgt tctggcatct taataacaat
     9541 ttttctcttt ataaagaagg tataaatact tcctactacc aaacctataa tgatagcacc
     9601 gataattcct tggcctccaa accaaacttt actaatagcg tccccaatcg cctcaccttg
     9661 tttagggata taagatgatc ttagcaaaat aaagaatgca gatacagata gaactccagc
     9721 tggtaaagcc tctactccgc tattcttagc ataagaatag gcaattgaaa aacaagaaat
     9781 tagacccata atagcaaaag ttcctgaata tacttgcata aacggctctg tccaattagc
     9841 tccaaaaaca ctagcaatgc tcttatttaa tccttcgaac ggcaattgtc ccataatcaa
     9901 gaacaaactg ccaactactg tcaatggcaa aattgctaac atcccatctt ttagagctat
     9961 aatgccacgc atattcacaa acttcatcat cggtgcaatg attttctgaa catccatctt
    10021 tgacataata aatctccttt tcttacccac taatcaaaga tagggccaaa tctaatactt
    10081 ttttcccatc taacatacca tagtccatca tcggaataac agctatcgga acatcacact
    10141 tatcacaaat ttcttttgat ttatctaatg tataagcaac ttgtggaccc aatagtgcaa
    10201 catctatatt tggcgcataa tccgctaatt tagactgaga aaacgcctct atttctgcct
    10261 caactccact agattgcgct gcaattttca tattatttac aagcatacca gtagaaaaac
    10321 ctgctgcaca aaacaaacca atcttcacca ttatgttttc ctcctctatg ttaataacaa
    10381 tgataatact ctagtattat tttttatgaa gttcttttct caaactaata atttctttga
    10441 ttaaattaat ctccgtcata ctagtcatga gatgatcttg tgaatgaaca aagagagctg
    10501 taatttctgt ctttgtacca gacgcttcct gtgccaagaa ttttgtttgt agattatgag
    10561 cttctaataa ggctgcatca gctaactgga tttcttcttc acaccgttca catgtaccat
    10621 tttttatgta atctaacgct ttataaatat gttgtttcgc atcaccagca tataaaatta
    10681 aacccatgat aatttgatct ggaacaataa tttccataaa ttcctcctca tttcactatg
    10741 taaaaagctt ttttcatttg aaattttaaa attttctttt gtaactatag aatacaacta
    10801 cataataaag ttgtcaatcc aaaaacaaca gataaactat ttttgtttat ataagcacaa
    10861 atgtgcaatc aactaaaaag aaaaacgata aactgaatca gtctatcatt ttcactcacc
    10921 tagaatctct ctaaaagtca aatccccatc ttcttccaaa acttttaaaa ttgtattttt
    10981 gtctgtaatc aaatgattta ctaaattagc acgcaagaca gaaagaatcg aagattcttt
    11041 cgtatcaccg taagcaacag ctaaactttg aggaatattt tttaaatctt ccaaagagat
    11101 tgctatcgtt ctttcctgta agttttcata gacttcttta ccttttgaat caaaaaatcg
    11161 acaacaaatt tctcctacag ttttaacttt ggtcaattct ttaaagtcat cctctgtaag
    11221 catgtctaac cattgatgct ttcctttatt gctaaaatca ccaattccga ctacagctat
    11281 atctaaatct ttccaactat ttttcaaatt ttcaaaatat cttgattgca aaataccatc
    11341 tgctaacaat ttattttctt gcacaatcgt tgcattcata aatgtacact ctccatgaaa
    11401 ttttctagac atttcataaa tcagtgtatt cacatggtat ttagcgtgta tgtgactagg
    11461 accacctgct agaggataga agtgaacatt tcggacactt ttactgtgaa ttaaatctac
    11521 taaattactt aaacttttcc cccaagaaaa gccaattttc atattatcat caattagatt
    11581 cctaaggaca cctgctgcaa cttgagaaat tctttcagat aaaattgttg gattatcatc
    11641 aaattcattt ggaataattt ctaaactttc cagaccatat ttttctttta cataattttc
    11701 caacttaaac atattggtat caaaattctc tatttcaatt ttaacaattc ctgcattcct
    11761 tgcttctgtt aacattctac taatagaggt tctataaatt cctaattttg ctgctatttg
    11821 tgactgattt aagttttcaa tataatacag ataagcaatt ttagagagca gtttattcct
    11881 atcttgattc atacacttaa cctcttacga aactacctta accattatcc cagcattttc
    11941 taatgtagcc atattttgtt tagaaagttt ttcgtctgtt attacttcat agacttgact
    12001 taaagcaaat cttcttactg tacccctttt atcaaattta cttgagtcag ttagcacaat
    12061 gactttatcc gacgctgctg aaatatattg aactacctca ctgcgcatta aatcttttcc
    12121 tgtaaagccc atctctttat cgtagccatc tgtcccaaca aaagcttgac gcacatgaaa
    12181 agtctgtatc atttctttta ataaaggtcc cacagtaacc tgtgaatcct tctgaaactc
    12241 tccaccaaga acaataatac gacatgaatc ataagctctc acaaaatttg ctataaaaaa
    12301 tgaatttgtt acaatcgtaa catttctttt ttgcttgcaa atttcctcag caagtaaagc
    12361 acaggtcgat ccagattcta tcattattgt ttcattatct gacaccaatt ttactgcttc
    12421 ctgaacaatt tttctcttaa tttcatagtt aattgacaaa cgtacattta agtcatctcc
    12481 actatttaat acagcatatc catgctctct gtgtaataaa ccttttgact ctaatttatc
    12541 taaatctttt ctaatcgtta ctttcgat
//
LOCUS       DAVMHG010000048        12546 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMHG010000048 DAVMHG010000000
VERSION     DAVMHG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.048"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            64..753
                     /gene="coaB"
                     /locus_tag="VYR08_001099"
     CDS             64..753
                     /gene="coaB"
                     /locus_tag="VYR08_001099"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV3466637.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQTKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            765..1316
                     /gene="coaC"
                     /locus_tag="VYR08_001100"
     CDS             765..1316
                     /gene="coaC"
                     /locus_tag="VYR08_001100"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV3466638.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1300..1863
                     /locus_tag="VYR08_001101"
     CDS             1300..1863
                     /locus_tag="VYR08_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3466639.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2145..2672
                     /locus_tag="VYR08_001102"
     CDS             2145..2672
                     /locus_tag="VYR08_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3466640.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2684..3199
                     /locus_tag="VYR08_001103"
     CDS             2684..3199
                     /locus_tag="VYR08_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV3466641.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALEQLGYLVKD"
     gene            complement(3196..3663)
                     /locus_tag="VYR08_001104"
     CDS             complement(3196..3663)
                     /locus_tag="VYR08_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV3466642.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLRS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYRHRTEDDWEKKIF"
     gene            complement(3716..4236)
                     /locus_tag="VYR08_001105"
                     /pseudo
     CDS             complement(3716..4236)
                     /locus_tag="VYR08_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4202..4765)
                     /locus_tag="VYR08_001106"
     CDS             complement(4202..4765)
                     /locus_tag="VYR08_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466643.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(4775..6763)
                     /gene="uvrB"
                     /locus_tag="VYR08_001107"
     CDS             complement(4775..6763)
                     /gene="uvrB"
                     /locus_tag="VYR08_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV3466644.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6840..7793)
                     /locus_tag="VYR08_001108"
     CDS             complement(6840..7793)
                     /locus_tag="VYR08_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3466645.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSSLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLVLFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            7966..10131
                     /locus_tag="VYR08_001109"
     CDS             7966..10131
                     /locus_tag="VYR08_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3466646.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            10131..10871
                     /locus_tag="VYR08_001110"
     CDS             10131..10871
                     /locus_tag="VYR08_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3466647.1"
                     /translation="MAKLKIDVNDLHKYYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            11020..12507
                     /locus_tag="VYR08_001111"
     CDS             11020..12507
                     /locus_tag="VYR08_001111"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV3466648.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
BASE COUNT         3810 a         2727 c         2283 g         3726 t
ORIGIN      
        1 gtttttacca aaaatttatc actttcattt tacttaccgc ttatttttgt gtacaatagt
       61 gctatgaaaa ttttagttac atcgggcggt accagtgaag ctatcgatag cgtccgctct
      121 atcactaacc attctacagg tcacttgggg aaaattatca cagagacttt gctttctgca
      181 gggcatgaag tttgtttaat tacgacaaaa cgagctctga agccagagcc tcatcctaac
      241 ctaagtattc gagaaattac caataccaag gaccttctaa tagaaatgca agaacgtgtt
      301 caggattatc aggtcttgat ccactcaatg gctgtttctg actacactcc tgtttatatg
      361 acagggcttg aggaagttca ggctagctcc aatctaaaag aatttttaag caagcagaat
      421 catcagacca agatttcttc aactgatgag gttcaggttt tgttccttaa aaagacaccc
      481 aaaatcatat ccctagtcaa ggaatggaat cctactattc atctgattgg tttcaaactg
      541 ctggttgatg ttaccgaaga tcatctggtt gacattgcac gaaaaagtct tatcaagaat
      601 caagcagatt taatcatcgc gaatgacctg actcaaattt cagcagatca gcaccgagct
      661 atatttgttg agaaaaatca gcttcaaaca gtccagacta aagaagaaat tgcagaactc
      721 ctccttgaaa aaattcaagc ctatcattct tagaaaggaa aactatggca aacattctct
      781 tggctgtaac gggttcaatc gcctcttata agtcggcaga tttagtcagt tctctaaaaa
      841 aacaaggcca tcaagtcact gtcttaatga ctcaggctgc tacagagttt atccaacctt
      901 tgacactaca ggtactctca cagaatcctg tccacttgga tgtcatgaaa gaaccctatc
      961 ctgatcaggt caatcatatc gaacttggaa aaaaagcaga tttatttatc gtggtacctg
     1021 caactgctaa cactattgca aaactagctc acggatttgc ggacaacatg gtaaccagta
     1081 cagctctagc cctaccaagt catattccca aactaatagc tcctgctatg aatacaaaaa
     1141 tgtatgacca tccagtaact cagaataatc tgaaaacatt agaaacctac ggctatcagc
     1201 tgattgctcc taaggaatcc ctactagctt gtggagacca cggacgagga gctttagctg
     1261 acctcacaat tattttagaa agaataaagg aaactatcga tgaaaaaacg ctctaatatt
     1321 gcacccattg ctatcttttt tgctaccatg ctcgtgatac actttctgag ctcacttatc
     1381 tttaaccttt ttccatttcc aatcaaaccg accattgttc atattcctgt cattattgcc
     1441 agcattattt atggtccacg agttggggtt acacttggat ttttgatggg attacttagc
     1501 ttgacggtta acacgattac gattctaccg acaagctacc tcttctctcc cttcgtacca
     1561 aacggaaaca tctactcagc tatcattgcc attgtcccac gtattttgat tggtttaact
     1621 ccttacttag tctataaact gatgaaaaac aagactggtc tgattttagc tggggctctt
     1681 ggttccttga caaatactat ctttgtcctt ggaggaatct tcttcctatt tggaaatgtt
     1741 tataacggaa atatccaact tcttctggca accgttatct caacaaattc aattgctgaa
     1801 ttggtcattt ctgcaattct aaccctagcc attgttccac gactacaaac cttgaaaaaa
     1861 taaaaacaat ctccgctttc aaacctgagg gtggagattt tgctctatac agtttctttt
     1921 taatgaaatc tttactatag taaaacgaaa taagaacagg acaaattgat caggacagcc
     1981 aaatcgattt ctaacaatgt tttagaagta gaggtggact attctagttt caatctacta
     2041 taacttttaa ctaaaaaatg atctataaac ccaagtaaat tcttgcttta ttgtctggtt
     2101 tattgtacca tactagtgta tatgcagtta aaaaggagat tcttgtgaat acacggaaaa
     2161 agacacaatt tatgacaatg acagcccttt taacggctat tgcgattttg attccaattg
     2221 ttatgccttt caagattgtc attccacctg cttcctatac tttggggagc cacatcgcta
     2281 tttttatagc catgttcttg tcgcccttga tggcagtttt tgtcatccta gcctctagtt
     2341 ttggattttt gatggctggc tatcccatgg ttatcgtttt tcgggctttt tcccatatat
     2401 cttttggtgc tttaggagct ctttacctac aaaaattccc cgatacccta gataaaccaa
     2461 aatcttcctg ggttttcaac tttgttttgg ctgttgttca tgcccttgct gaagtattag
     2521 cctgtgtcct tttttacgca acttcgggta ccaatgtaga aaacatgttc tatgttctat
     2581 ttgtgctagt tggatttggc acaattatcc atagtatggt agactataca ttagcactag
     2641 ctgtctataa agtgcttcga aaacgccgtt aaaaggaaaa gctatgacaa aagatcgcaa
     2701 acaagccctg ctccaactct tgaaagaagc tcctaaagcc cttaatggcc aaagtttggc
     2761 ggaacatttt catgtcacac gtcaggtcat tgtgcaggac attgcaattt tgagagccga
     2821 tggcgctcct atcctatcca ccaatcgtgg ctacatctat aagcaaattg ataccaatcc
     2881 ttatgttcac aaacttttca aagtgaaaca tgaagttgaa gaaatcggtc aggaactcct
     2941 tgctattgtc gataatggtg ggcatgtcca gaataccttg attgaccatc ccgtttatgg
     3001 agaaattgaa accttgctta aactgtcttg tcgcagagat gtgcaacatt ttctagaaca
     3061 agtcgagcat tctgacttta gacctctatc tgaattgaca gatggcatcc attaccacct
     3121 agtcgaagcc gaaacacaac aagacctcca ctatatcgag gaggccttgg agcagctcgg
     3181 ttatttagta aaggattaga aaattttctt ttcccaatcg tcttctgtac gatgacggta
     3241 gaaaaactct gatttgtccg gagcttccat catctccatc agaggtagca tatcataggc
     3301 cagatccaag tttggaatct ggtctttttg cacccaagaa acttctccct cttctgaaga
     3361 gcgaagggta ccagagaact cagtcgcctt ataacaaata acaatgtagc gcccacctgt
     3421 atctagtggc caatttttaa tgccgacaag ttgaggattt tggatagtca accctgtttc
     3481 ttcatagatt tcacgaatga cagactccgc aaaagcctca tcattttcta catgacctcc
     3541 aggaaaggca taacctgacc agcgattggt gtcaggagag cgatactgca tgactacgcg
     3601 ctgagtttct aagtcttcaa tcagacaaat attggttaaa atcgttaatt gggaacggga
     3661 cataaattta ctcgctttct aatttattaa attttcagac ttggtaccag tgccattagc
     3721 ttctactttt taaaattatt ttagaataga ataccattcg tttttccttt ataggctttc
     3781 caagttcaag tatcttttct tgtccatcac ttgtccatca aaagtaaaac ccattttttg
     3841 atagaaagca atggcgcgct tgttatcttt caatacccat aagaaaattt cagaaaaatg
     3901 attaagagca gtcaaagctg ctttcattaa cttttgtgct atgccttttc cgtaatagtc
     3961 ttttaaaaca tataaagcaa taatttcacc agcttgaata gtctcatcac gaaagttacc
     4021 ataactgata aaaccaacta ccttcagatc acctatcgca atcaatgtat tttctggata
     4081 cttttgacta aagagtcgac atctttctaa tgtcattgtc tcctgaaatt ctgcaggcaa
     4141 aaggtcatca taagcctctc tccacatttg ccagtgaaca agggatttac cttctatctc
     4201 tttaggagtt tccattgatt tgataataac cgtcatttat tttctcccag tcttctctca
     4261 aaataccata tttaatacta tcaaaatatt taccttgata ataacgaact tttggaatat
     4321 gagcttcttt tttcattctt aatttttcag caagtttcat cataccaaga ttccctgacc
     4381 aagttgtcaa acccagatgc tccaactcca agtaatcctg aaacgtccta tctatccact
     4441 gcaacatagc aactttccca ataccagtgt tccagaattt tttatcataa ataccaattc
     4501 ccaattccat ccatcttgtt tctttacata cccaataacg cgaaacagtc ccaactagtt
     4561 tatcatcaac aaaaatacca aggcgatttg agttgcttaa aatggattct gatttttgta
     4621 gttcgaattc tttaaaattt gaaaaatact gataatcatc ataataggga gcatcatact
     4681 gtttccaaac tggattaagt tgtgaataag ccatttccca taaagacatc aaatcttctc
     4741 tctttaactt tcttaaataa atcatactat tcccctaatc caaggccttg acttccaaca
     4801 tcatatcacg gatctgagcc gccagttcaa agtcaagcac ttcgacggct tcttgcattt
     4861 gtttttctag tttcttgacg agttctttgc gctcttgttt gttgaggcta ttgatgtcga
     4921 cttccttgtc ctcttcctta gcaactgcct tggtcacggc aatcaggtca cggatttctt
     4981 tcttgattgt ctgtggtacg ataccatgct cttcattata ggccatctga attttccgac
     5041 ggcgggcagt ttcatcgata gcacgttgca tagactgggt aaccgtatct gcatacatga
     5101 tgacatgacc ttcactgtta cgggcggcac gcccaatggt ctggatgagt ccacgctcgt
     5161 tacgaaggaa accttccttg tcagcatcga gaatggcaac caagctaacc tcaggaacgt
     5221 caatcccttc acgaaggaga ttgattccaa ccaagacatc aaagacaccc aagcggagat
     5281 cacggataat ctccgtccgt tccaaggtct tgatatccga gtgcatgtac ttgaccttga
     5341 tgcccatttc cttgaaatag tcggtcaagt cctctgccat tttcttggtc aaggtggtga
     5401 taaaggttcg ttcattcttt tcaacacggg cattgatttc acctaagaga tcatcaatct
     5461 gtcccatagt cggacggact tccacctcag gatccaaaag ccctgtcggt cgaatgattt
     5521 gctcaatcac cgtctcagtc tgttcatttt catagtcacc aggtgtcgct gatacgtaaa
     5581 caatctgatg cacatggctt tcaaactcct cacgacggag aggacgattg tccaaggcag
     5641 acggtaaacg gaaaccataa tttactagca tttccttacg cgaacggtct ccattgtaca
     5701 tgccttttat ttgtcccata gtcatatgac tctcgtcaat cataatcaaa aaatcatctg
     5761 ggaaaaagtc gagaagcgta taaggaggct caccttcact acgtccatcc atgtggcgag
     5821 aataattttc aaccccattt gtatagccca tctcgcgcag catttcgata tcgtactctg
     5881 tccgttgttt caagcgctgg gcttcaagca atttaccttc cttctcaaag acagctaact
     5941 gctcttctaa ttctgcttga atcttggcaa cggcaacttc catatggtcg tcattggtca
     6001 caaagtgagt agctgggaaa atcgccaaat gatccacctc tcccaatacc tgacctgtca
     6061 gagcctcaac ctcacggata cggtcaattt cgtctccaaa aaattctact cgaaaggcat
     6121 gttcatctcg ggaagctggg aaaatctcta ccacatcccc acgaacgcga aatcttccgc
     6181 gttggaaatc aatatcatta cgttcaaact gaatatcgac caagtcattc aagagtttat
     6241 cacgagaaat ctctagacca ggacggagac taacgacact atcagcgtat tccttgggcg
     6301 aacccaaacc atagatacaa gagactgagg ccacgacaat aacatcatta cgctccaaaa
     6361 gggctgaggt agctgagtgg cgaagcttgt caatctcgtc attgacagaa ctatccttct
     6421 caatataggt atcgctagaa gggacatagg cctctggctg gtaataatca tagtaggata
     6481 cgaaatactc aactgcattt tcagggaaaa attccttaaa ctccccatag agctgaccag
     6541 ccagagtttt attgtgggca ataaccagag ttggtttatt gactttagaa atgacctgac
     6601 tcatagtata ggtcttccct gttccagtcg cccccatcag aatctgagct ttttctcccc
     6661 cctcaatgtt atccaccaac tgctcgatag cttggggttg atctcctgat ggttgatatt
     6721 ttgatactag tttaaattga ttatctgtaa tgtgattgat cataagaatc ctctctccat
     6781 gtgctattcc tattttagca tacttatagt attttcacga agaaaaggac ggaccgaagt
     6841 cacatccttt cattttcttt ctttaattta ttagcaagat agacaatcag cagtagtaat
     6901 accagacttg tcataatgga accttctatg ccaaaagaac cacctgatag ccaatcttga
     6961 ttgccttgtg gtaaaaaggt catcagagac gttcctgacg gttgaccact aactaaaatc
     7021 ccaaagagat taccctgagc aaaattccaa gcaccatgaa tacctgcaac accccaaact
     7081 gtatcagttt tgagaaggta aagagccatg gcaactccga ataaaaagag atttactaga
     7141 gatagagggg tgaggccaga attgcccata tgaagcaggg taaagaacag gctagatata
     7201 agaatagcta gttttagatt ggttcttgag gccaattgag gaagtagcca agcacgggcc
     7261 accacttctt ctgctgtccc ctgtaaaatc caaaatggga tagtaaagac gacaaaggca
     7321 agagaataag gattcaagtg aatggattcc aaacgatatt gacctaagac caccaaacct
     7381 aacaaggtca gaagaaaaag taccaggcct agactaaatc ctttcagaag attgctgagg
     7441 caattctctc tataaaatcc caaggttcga ataggtcttt tctcaacttt tcttgtccat
     7501 ctgaacacag tgttaagaat aaaaccaaag gccagtaatc ctaaaattaa acgaaaatca
     7561 cttgttttat ccgtcaagct ctgatgcaag gtctgcaagt agcttgcaag attttgacca
     7621 gcctctccaa aatttctagc aagtccgatg agagctaaca aactaatacc atataaagta
     7681 taagccacaa actctcctat gaagacaaaa acaaaggcta acaaggagct tgtgtaaatg
     7741 tttaaattca tttttgaagc ttggaagtct ttaaatattc ttttgttctt catatccccg
     7801 tcctcttttc ttctatatat actattatta tagcactttt taatagcaat tcaagaaaga
     7861 aaaggaaaaa tcaatgttat attttctgac acgaaaaata taaaaatttg ccttttttta
     7921 ctttttctga tataatgaga gaatattcgg aaaaggagac taaaaatgaa gaaaaaattt
     7981 ctagcatttt tgctaatttt attcccaatt ttctcattag gtattgccaa agcagaaacg
     8041 attaagattg tttctgatac cgcttatgca ccttttgagt ttaaagattc agatcaaact
     8101 tataaaggaa ttgatgttga cattattaac aaagtcgctg agattaaagg ctggaacatt
     8161 cagatgtcct atcctggatt tgacgcagca gtcaatgcgg ttcaagctgg gcaagccgac
     8221 gctatcatgg cagggatgac aaagactaaa gaacgtgaaa aagtcttcac catgtctgat
     8281 acttactatg atacaaaagt tgtcattgct actacaaagt cacacaaaat tagcaagtac
     8341 gaccaattaa ctggcaaaac cgttggtgtt aaaaacggaa ctgccgctca acgtttcctt
     8401 gaaacaatca aagataaata cggctttact attaaaacat ttgacactgg tgatttaatg
     8461 aacaacagct tgagtgctgg tgccatcgat gccatgatgg atgacaaacc tgttatcgaa
     8521 tatgccatta accaaggtca agacctccat attgaaatgg atggtgaagc tgtaggaagt
     8581 tttgctttcg gtgtgaaaaa aggaagtaaa tacgaacacc tggttactga atttaaccaa
     8641 gccttggctg aaatgaaaaa agatggtagt cttgataaaa ttatcaagaa atggactgct
     8701 tcatcatctt cagcagtgcc aactacaact actctcgcag gattaaaagc tattcctgtt
     8761 aaggctaaat atatcattgc cagcgattct tcttttgcac cttttgtttt ccaaaattca
     8821 agcaaccaat acactggtat tgatatggaa ttgattaagg caatcgctaa agaccaaggt
     8881 tttgaaattg aaatcaccaa ccctggtttt gatgctgcta tcagtgctgt ccaagctggt
     8941 caagccgatg gtatcattgc tggtatgtct gtcacagatg ctcgtaaggc aacttttgac
     9001 ttctcagaat catactacac tgctaatacc attcttggtg tcaaagaatc aagcaatatt
     9061 gcttcttatg aagatctaaa aggaaagaca gtcggtgtta aaaacggaac tgcttctcaa
     9121 accttcctaa cagaaaatca aagcaaatac ggttacaaaa tcaaaacctt tgctgatggt
     9181 tcttcaatgt atgacagttt aaacactggt gccattgatg ccgttatgga tgatgaacct
     9241 gttctcaaat attctatcag ccaaggtcaa aaattgaaaa ctccaatctc tggaactcca
     9301 atcggtgaaa cagcctttgc cgttaaaaaa ggagcaaatc cagaactgat tgaaatgttc
     9361 aacaacggac ttgcaaacct taaagcaaac ggtgaattcc aaaagattct tgacaaatac
     9421 ctagctagcg aatcttcaac tgcttcaaca agtactgttg acgaaacaac gctctggggc
     9481 ttgcttcaaa acaactacaa acaactcctt agcggtcttg gtatcactct tgctctagct
     9541 cttatctcat ttgctattgc cattgtcatc ggaattatct tcggtatgtt tagcgttagc
     9601 ccatacaaat ctcttcgcgt catctctgag attttcgttg acgttattcg tggtattcca
     9661 ttgatgattc ttgcagcctt catcttctgg ggaattccaa acttcatcga gtctatcaca
     9721 ggccaacaaa gcccaattaa cgactttgta gctggaacaa ttgccctctc actcaatgcg
     9781 gctgcttata tcgctgaaat cgttcgtggt ggtattcagg ccgttccagt tggccaaatg
     9841 gaagccagcc gaagcttggg tatctcttat ggaaaaacca tgcgtaagat tatcttgcca
     9901 caagcaacta aattgatgtt gccaaacttt gtcaaccaat tcgttatcgc tcttaaagat
     9961 acaactatcg tatctgctat cggtttagtt gaactcttcc aaactggtaa gattatcatt
    10021 gctcgtaact accaaagttt caagatgtat gcaatccttg ctatcttcta tcttgtaatt
    10081 atcacacttt tgactagact agcgaaacgc ttagaaaaga ggattcgtta atggcaaaat
    10141 taaaaattga tgtaaatgat ttacacaagt actatggaaa aaatgaagtc ctaaaaggaa
    10201 ttacgactaa gttctatgaa ggagatgttg tttgtatcat cggtccttca ggttctggta
    10261 agtcaacttt cctccgtagc ctcaatcttt tagaagaagt cactagcggt cacatcactg
    10321 tgaacggcta tgatttaact gaaaaaacaa ccaatgttga ccacgtccgt gaaaatatcg
    10381 gcatggtatt ccaacacttc aacctcttcc ctcatatgtc tgtattggac aacatcacct
    10441 ttgctcctat tgagcacaag ttgatgacta aggaagaagc tgaggaattg ggaatggagt
    10501 tgcttgaaaa ggttggacta gcagataaag ctaatgccaa tccagatagc ctatcaggtg
    10561 gtcaaaaaca acgtgtggcc atcgctcgtg gcctagcaat gaatccagac atcatgctct
    10621 tcgatgaacc aacttctgcc cttgaccctg agatggttgg agacgtactt aacgttatga
    10681 aggaattggc tgagcaaggc atgaccatga ttatcgtaac ccatgagatg ggatttgctc
    10741 gtcaggttgc caaccgcgtt atctttactg cagatggcga gttccttgaa gacggaacac
    10801 ctgaccaaat ctttgataac ccacaacacc ctcgtctgaa agagttctta gataaggtct
    10861 taaacgtcta aactcaaact gtaaggattt ccttgcagtt tttctacctc gtattggaat
    10921 ttttgatttt tcggaaaatt atgttagaat taagtttatg aaatgaggtt tcctcatacc
    10981 tagcaagact aggaataaaa atagaaatta ggtagctaga tgtcatctaa ggttattgtt
    11041 acaattttcg gtgcgagtgg agacctggct aaacgcaagc tctacccttc cctttttaga
    11101 ctatatcaat ccggcaatct ttccaagcac tttgccgtta ttggaactgc ccgtagacct
    11161 tggagtaagg aatattttga atctgtagtt gtcgagtcca tccttgattt ggcagatagt
    11221 accgagcaag cccaagaatt tgctagccac ttctactatc aaagccatga tgtcaatgat
    11281 tcggaacatt atattgcttt gcgtcaatta caagctgagc ttaatgaaaa ataccaagct
    11341 gaacacaata agctcttctt cttgtctatg gcacctcagt tctttggaac cattgccaaa
    11401 cacctcaaat ctgaaaacat tgtcgatggc aaaggttttg agcgcttgat cgttgaaaaa
    11461 ccatttggta cagattacgc aactgcaagc aagttgaatg acgaactcct agcaacattt
    11521 gacgaagaac aaattttccg tatcgaccat tatcttggta aggaaatgat ccaaagcatc
    11581 tttgcagttc gctttgcaaa cttgattttt gaaaacgttt ggaacaagga ttttatcgac
    11641 aatgttcaaa ttacctttgc ggagcgcttg ggtgtagaag aacgtggtgg ctactatgac
    11701 caatccggtg ccctccgtga catggtccaa aaccacactc tacaacttct ttcgctcctc
    11761 gccatggaca aaccagcaag cttcacaaaa gacgagattc gtgctgaaaa gattaaggtc
    11821 tttaaaaacc tctatcatcc aactgatgaa gaactcaaag aacactttat ccgtgggcaa
    11881 taccgctctg gtaagattga tggcatgaaa tacatctctt atcgtagcga gccaaatgtg
    11941 aatccagaat caacaactga aacctttaca tctggtgcct tctttgtaga cagcgatcga
    12001 ttccgtagtg ttcctttctt tttccgtaca ggtaaacgac tgactgaaaa aggaactcat
    12061 gtcaacatcg tctttaaaca aatggattct atctttggag aaccacttgc tccaaatatt
    12121 ttgaccatct atattcaacc aacagaaggc ttctctctta gcctaaatgg gaagcaagta
    12181 ggagaagaat ttaacttggc tcctaactca cttgattacc gtacagatgc gactgcaact
    12241 ggtgcttctc cagaaccata cgaaaaattg atttatgatg tcctaaataa caactcaact
    12301 aactttagcc actgggatga ggttggtgcg tcatggaagt tgattgaccg tattgaagag
    12361 ctctgggctg aaaatggtgc cccacttcat gactataaag ctggaagcat gggacctcaa
    12421 gccagctttg acctacttga aaaattcggt gccaaatgga cttggcaacc agatatcgcc
    12481 tatcgtcaag atggtcgttt cgaataaaaa aatttcctgc aagtttatgc cttgcaggat
    12541 ttggtt
//
LOCUS       DAVMHG010000049        12531 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMHG010000049 DAVMHG010000000
VERSION     DAVMHG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.049"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(28..939)
                     /gene="whiA"
                     /locus_tag="VYR08_001112"
     CDS             complement(28..939)
                     /gene="whiA"
                     /locus_tag="VYR08_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV3466649.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(936..1913)
                     /locus_tag="VYR08_001113"
     CDS             complement(936..1913)
                     /locus_tag="VYR08_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV3466650.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(1910..2800)
                     /gene="rapZ"
                     /locus_tag="VYR08_001114"
     CDS             complement(1910..2800)
                     /gene="rapZ"
                     /locus_tag="VYR08_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV3466651.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(2852..3232)
                     /locus_tag="VYR08_001115"
     CDS             complement(2852..3232)
                     /locus_tag="VYR08_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV3466652.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(3243..3830)
                     /locus_tag="VYR08_001116"
     CDS             complement(3243..3830)
                     /locus_tag="VYR08_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV3466653.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(3839..5071)
                     /gene="clpX"
                     /locus_tag="VYR08_001117"
     CDS             complement(3839..5071)
                     /gene="clpX"
                     /locus_tag="VYR08_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV3466654.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(5103..5273)
                     /locus_tag="VYR08_001118"
     CDS             complement(5103..5273)
                     /locus_tag="VYR08_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466655.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(5273..5779)
                     /locus_tag="VYR08_001119"
     CDS             complement(5273..5779)
                     /locus_tag="VYR08_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV3466656.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFGPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(5909..6427)
                     /locus_tag="VYR08_001120"
     CDS             complement(5909..6427)
                     /locus_tag="VYR08_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV3466657.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(6924..8429)
                     /gene="lytC"
                     /locus_tag="VYR08_001121"
     CDS             complement(6924..8429)
                     /gene="lytC"
                     /locus_tag="VYR08_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV3466658.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(8467..9225)
                     /locus_tag="VYR08_001122"
     CDS             complement(8467..9225)
                     /locus_tag="VYR08_001122"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV3466659.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(9323..10000)
                     /locus_tag="VYR08_001123"
     CDS             complement(9323..10000)
                     /locus_tag="VYR08_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV3466660.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(10009..10953)
                     /locus_tag="VYR08_001124"
     CDS             complement(10009..10953)
                     /locus_tag="VYR08_001124"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV3466661.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(11135..11647)
                     /locus_tag="VYR08_001125"
     CDS             complement(11135..11647)
                     /locus_tag="VYR08_001125"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV3466662.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(11734..12492)
                     /locus_tag="VYR08_001126"
     CDS             complement(11734..12492)
                     /locus_tag="VYR08_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV3466663.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKFAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
BASE COUNT         3567 a         2934 c         2249 g         3781 t
ORIGIN      
        1 accacgagtc acataggatt cgtggtttta taattcatcc gctatcttat tgatttttct
       61 gagtctgtga ttgacaccac ttttggtcag aggggtgctg agactatctg ctaactgctg
      121 gatagagtag tctgggtgct gaatcctcag ttgcgccact tcctgcaaat ctactggcaa
      181 attttctaag cccatgatat ctttgatttt actgatattg ttaatggtct tcatgctggc
      241 agaaactgtc cgagcgatat tagctgtctc ggcattatta gcccgattga ggtcgttacg
      301 ggtttctcgc aaaatcttaa cccgctcaaa atcatcacgt gcctgcatgg ctccgattac
      361 tatcaagaag tccataatgt cttctgctcg ctggagatag gtcacagccc ccttcttgcg
      421 ctcaagcacc ttggcatcca gtaaaaactg ttggagaagg gaggcaattc cttgcgcgtg
      481 gtccagataa acagaactga tttccaactg gtacttgcct gactcagggt cacgaatgct
      541 cccatttgcc aagaaagcgc cacagagata ggcacgacct gcttcctcat ccgataaaat
      601 cgcctcatca atacctgttt ccaggccaaa gaaagagtct gccaagtgca aatcacttaa
      661 caaatcctgc accttttcat ctgtaaaaac ggtatagacg cgattcttgc gaagattgct
      721 ccgttggtgg tgacgaattt cagatttgat ttcatagaaa tggagaaagg actcatagag
      781 gtgacgggcc agtttggcat tttctgtcac aactgacaaa gtcaagcccg aagtcgagag
      841 accgatgcta ccagacattt tgataatggc agataattca tgccagctca gatggtgttg
      901 gcccaggatt tcttctttta ctgctactgt gaaactcatt ttttcacctg tataatgcgc
      961 atcaactcgt ccacaatcaa atctccatcg tggaaggcac cgccattttc cagacgaagg
     1021 aagttagatg aaatcacgcg cgaaacttgc ttacaaagac ctacaaaatc gtgttccact
     1081 tgcactaagt attcatcaaa acggttggaa ttcatgtatt cctgaggcac tttttcaata
     1141 ttcaccaaga cagtgtcgat aaaagggcga ccaaggtgac gatgcaagac ttccacgtgg
     1201 tcgctatctg taaagtgttc cgtctcccca cgttgggtca tgatattgca gacataggca
     1261 atttctgcct tggtttccaa aagagcccgc ccaatttcct taatcacgat attgggcaaa
     1321 atagaggtaa agagggaacc tggccctagg acaatcatgt cactttcaag gatggtctgc
     1381 actactcgac ggctggccag aggcgtatca tcgtttaggg cattggtcac atagacattg
     1441 tcaattatgc ctcgatggtc tacaatatga ctctctccag ccacttctgt cccatcctga
     1501 aagactgcat gaagggtcaa aggatggtca ctggaaggat aaattttccc tgttgtatgg
     1561 aaaaatttgc tcaataactg catggcatta taggttgaac cctgcatttc tgacaagcca
     1621 gcaatgatga gatttcccaa tggatggcca gcaaaggctc cggcatcctc agagaaccga
     1681 tactgaaaga ccttctcata aaacttaggc atatccgaca tggccacaag gacattacga
     1741 agatcacctg gcggtgtcaa ctgttgcata ttttttcgga gttcacctga agaaccacca
     1801 tcatctgcca ccgtcacgat agctgcgatt tccacatctt tttcccgcag actttttaga
     1861 atgacgggaa ttccagtccc tccaccaatc accgttatct ttggttttct catgaacggt
     1921 ttaccgtttc ctttctgcgg tctttgtcgc gatgcccttc attaacagac caattcttgg
     1981 ataagtcctg cgccaagcgt ttagcaaatg ccacactacg gtgttgtcca cccgtacatc
     2041 ccatggcaat ggtcaaaacg gacttacctt ccttttggta acttggcaga atcggctcaa
     2101 tcaaggccaa taaatgttga taaaagtctt ctgactcagg atggttcatg acataatcat
     2161 aaacaggttc atccacaccc gtttggtttc tcagttctgg tagataatag ggatttggca
     2221 agaaacggac atcaaagacc aagtccgcat caatcgggat tccatactta aatccgaaag
     2281 acatgacttc gatacggaaa gactgggctt gttcttggtc tgaaaactgc tctgcaaggg
     2341 ttttgcgcag ctcacgtgga gtgagttcag tcgtatccac cacattttgg ctcatatttt
     2401 tcaaaggtgc caagagttca cgttccaact tgattccatc taaaatacga ccgtctgctg
     2461 ctagtgggtg actccgtctg gtctccttgt aacgagcgac caattcctta tcagccgcat
     2521 ccaaaaagag gattttgaaa tccaaaccat cttgattttc caactcatcc aaaacagctt
     2581 gaatctctga aaagaaagaa cggctacgca tatccactac caaggccaac ttaggattgt
     2641 cttccttaat ttcaaccagc tgcaaaaact taggcaagag agctggcggc atattatcaa
     2701 tggtgaaata acctagatcc tcgaaggact gaatggctac agttttccct gcgccactca
     2761 tccctgtcac aatcaccaag tgaagttgtt tctttgtcat ctttttctcc ttatatcaaa
     2821 agaagtttgg caacaccaaa cttcaactag cttatccaat ctctgcgatg acttcaattt
     2881 cgacttttac atcacgagga agacgagcta cctccacagc tgaacgagct gggaattcct
     2941 ctttgaaggc cgtttggtaa acctcattaa aaggaacaaa gtcgttcata tcgctcaaga
     3001 agcaagttgt tttgacaaca tggtcaaagt ctgttcctgc ttctgccaaa atagcaccga
     3061 tgtttttcaa gacttgctct gtctgttctt ggatattctc tcctacaatt tccccagttt
     3121 caggggatag gggaacttga ccgctagcaa acaaaaggtt gccaacgatt tttccttgaa
     3181 catagggtcc gatagccttt ggggccttat ctgtatgaat tgtttttgcc attttctttt
     3241 cctcacaatt tttctaagat tgcatcccaa gcctcatcca tccctgcctt gctgacagat
     3301 gaaaagagga tgaaatcgtc acttgggtca aagtttaatt tctttttgat tgctgattca
     3361 tgcttgttcc atttaccacg aggaatcttg tccgccttgg tcgccacaat gatgactgga
     3421 atctcataat acttgagaaa ttcgtacatc tgcacatcat ctgctgacgg gtcatgacga
     3481 aggtcaacta gactgacaac cgcacggaga ttttcccgag tcgttaagta ctcctcaatc
     3541 atgcaccccc acttttcacg ttccttttta gaaacacgag catagccata accaggcaca
     3601 tccacaaagc gcatcttgtc atcaatgtta aaaaagttca ggagctgggt tttaccaggt
     3661 tttcctgatg tacgggcgag attcttacgg ttcaacatag tgttgataaa gctggattta
     3721 ccaacatttg aacgccctgc tagggcaatc tctggcagtt catcctgcgg atagtgggac
     3781 ttattagctg cactgagcaa gatttcagca ttgtgtgtat taagttccat agtcacctct
     3841 aggctgtttc taggatcggt ttatccgttc catctacagt ttctttagtg atgcgaacca
     3901 atttcacatt ttcctgactc ggcacctcaa acatgacatc tagcatggtt tcttcgatga
     3961 tggagcgaag tccacgcgcc cctgtcttcc gttcgattgc tttattagca atctcttgaa
     4021 gggcttcgtc gtcaaattcc aactcaacat catcataaga aagcaaggtt tggtattgtt
     4081 tcaccaaggc atttcttggc tctttcaaga tgcgaaccaa gtcatcaacg gtcaattgct
     4141 caagagccgc aaaaacaggc aagcgtccaa tcaactcagg gataatacca aatttttgaa
     4201 tgtcttcagc gatgatttct tgcatgtatg agctgttttc gtcaatcgcc ttattgtttt
     4261 gaccaaatcc gataactttt tcacccagac gttgtttgac aatttcttca ataccatcaa
     4321 aagcaccacc cacgatgaag aggatatttt ttgtatccac ttgaatcatc tcttgttgtg
     4381 gatgtttgcg tccaccttga ggcggcacgc tagcaacagt tccctcaata atcttgagaa
     4441 gggcttgttg caccccttca ccagaaacat cacgtgtgat agacacattc tcactcttct
     4501 tggcaatctt gtcaatttca tccacataga taatgccacg ctctgcacgt tcgatgttaa
     4561 agtcagcaac ctgcaagagt ttgaggagga tattttccac gtcctcaccc acataaccag
     4621 cctccgtcag agctgtcgca tccgcaatag caaaaggtac attcaagctc ttagccaagg
     4681 tctgggcaag gaaagttttc cctgaaccag ttgggccaat catcaaaatg tttgacttct
     4741 gcaaatctac atcttctgac tcttcgcgtg tatcgtggaa attgatgcgt ttgtagtggt
     4801 tataaaccgc cactgccaag gcacgcttgg cacgatcttg accaattaca tagtggttca
     4861 agatatggag gagttcaatt ggttttggca cctcagacaa gtctgccaag acttcctcaa
     4921 ccaattcttc tcgaatgatt tcctgagcta actccacgca ttcattacaa ataaaagcat
     4981 tgttgccagc aattattttt tgtacttctt cttggttttt gccacaaaat gagcaataaa
     5041 ccatcatatc attttttcta tttgtagaca tgatttcctt ccattctata ctgtcattct
     5101 atctaaaata aggtcatgta aaaagcatga atactattga ccagattggt aaaggcattt
     5161 aaccaaagga ggatagaaag cccgtaacgc tttttacgaa aagcctgtgc tcctgccaga
     5221 aagcagatga aacacagaaa agccgtgaat agaccaaata aactccgttc cattagactt
     5281 cctttctctt gcggtattgg atggtaaaat cataaggatt cttctcatct ttggcgtaaa
     5341 atttgcttga aactgtctca aaaagagaca agtcaagctc ttcagggaaa taggtatctc
     5401 cttccacccg agcatgaatg tgagtgacaa tcacttcatc aaggtaaggt ccaaaagcct
     5461 gaaaaatttg cttcccaccg ataatgtaga gattcttttc ttgagcctga taccagtcaa
     5521 gaacagactg gacgtcctga aaagtagcaa ccccatctat cttttcttcc ggattacgcg
     5581 tcaaaatcaa ggtttcccgt tttggaagca agcgacgtcc catcccatca aaggtcacac
     5641 gccccatcaa gatagcatga ttcagagttg tttctttaaa gtgctgcaat tctgctggca
     5701 aatgccaagg cagacgattt tccttaccaa tcacaccctc ttcatcctgg gcccaaatag
     5761 ctacgatttt cttagtcatg cttccatcct tttcactgat agtactattt tatcaaaaaa
     5821 ctcaaaaaaa gactggtttg gaatagctta caaaatagaa aaaatctgta agaaatttcc
     5881 tacagattta tctatgtttc cttatttctt acaaaccagg tgcttgtcca agttcggctg
     5941 caagcatcca aattgtttta tctgtttcag ttttagcgcc tgcaaagata ccgtttgtca
     6001 catcgtcacc ttcttcatca gtgacatcca aacctttttg gaaaagttct gacaagtaac
     6061 ggtagataac aagaacacgt tccaagcttt cttcaacatt acggtattca ccagcttctt
     6121 cttcgatttc actattttga aggaactctg tcaatgtaga gaatgggctt ccaccgagtg
     6181 taatcaagcg ttcgctgatt tcatccaatt gaccgtcaag agcttccatg tactcatcca
     6241 tttttggatg ccatacaagg aaaccacgac catgcatata ccagtgcact tggtgcaaag
     6301 caacgtgagc tacatacaaa tcagcaacag cttggttcaa gacttccttt gtttttgcca
     6361 atgctactgg cgctgctttt gtcaatgatg ttacgtcttt tactgcttct tttttcaatt
     6421 ctaccatttt ctttacctca ttcattattt tttgtaacca cttcttaatg attactacct
     6481 tagtatacta ctaaggaaaa ccaatagcaa gacaaagaac ttatatgaga aaagttgcaa
     6541 tagactgata atttaagaat tctttagaag acaaaaaaga gggttggatg cttatagtca
     6601 atggaaatcc atcagcaaac taggaaacta aacccagctt gaccaaagca caagttttga
     6661 ggtcgcaggt aggactgacc aagctgatgt ggtttgaaga aattccccaa tactataata
     6721 gattgaaata agatgaaacc aagtcgatta ggaaaggtaa atgaatttct aaaatgtttt
     6781 accaatcaca aggtctttat ctatattcaa tctactatat aagcttcatt tccgagcaag
     6841 atcttgaatt tactcgagga tgaactatca taaaacaaag aaaaagaggg tgctactatc
     6901 acatccctct ttcaaatcat cgcttaatac caaacgctga catctacgcg cccttggatt
     6961 cctttcatgt attcagaaga ggtatattgc caaccttttt ctcctgaata ataagggttt
     7021 tcccattcta aagcattcgt ataggccgct acccagttta catgttttaa aatatctggg
     7081 tgttttaaac gcgtctgtaa taaactacga tagctataga catacacatt ttgataaccc
     7141 gcctgcttca tcgtgtccat gtacttgttg atgattttaa cccaagtgtc tgtatcactt
     7201 ggagctctct tgctcttatt tacatattcc cagttctcaa catcataata gatagggtaa
     7261 gacaggttca tattgtattt ctttataagt tcaatggtct gtttagcgtc attctcagca
     7321 tcggtctcat tttcagcata ggtatagaga tagacaccat aaggaattcc cagacggttt
     7381 aactccttaa tgttatgcgc caattccttg tcttctttac cgctataacc tagacgaaca
     7441 atgacaccat ccacctcgtt ctcatcaata acctttttcc aatcattgat acgaccattg
     7501 tgctcactaa tatcaatgat tttcttagca tgttcggttc ccacttgttc ttctctatta
     7561 ttaaagaaat agcgtttacc atctctgtga acccagccga cattcaaatc tttcttttct
     7621 ttgagctcac cagaggccgc aaagatatag cgagtaccat ccataatcac ttcgtcagtc
     7681 gccatggcac cacttccagt caaatagtag ttgccttgcc actcattccg agccatatag
     7741 ccccacttct tgaaatagta ccatttgccg cccacttttt gccactcttg gttagcataa
     7801 gttccatcgg attttagata aaaataagca gaataggctg gatcatagag ccattcattt
     7861 tgcatcatag ctccttgacc attcaagaaa taacttcctt gccattggct tttagccatg
     7921 taaccccact tcttgaagta gtaccactta tttccaatca attgccattc ttgatgagca
     7981 taagccccat ctgacttgag ataaaaccaa ctcttgtaat tactgtcata gatccactcg
     8041 ttttgggcca tatatccacc tgatttcagg tagtaatttc catgccattc attctgcgag
     8101 taacgaccat ctgaattaat ataaaaccag gccttatagt agttgtcaaa aatccattca
     8161 ctttgagctt tagagccatc atcctttaca taataatccc catcccagtg ggcagcagca
     8221 ttagtctgta cttctgtgct cgtttgcgtt ttattagaag actgattttg ctctgaacta
     8281 cttgaagctg tcgttgtatc ctgcgaagtt tttgctactt cagtttcatt tgcagcgaca
     8341 tgactagttg ccaagcctag taaacagata cttgctaatc caatttttat tttcaatctt
     8401 tcctctccta taaaaaatgt aacagacatc tgaatgctgt tccacctagc ttttgctact
     8461 tactgattat tttacaaagt caagcaaagc caagaagctt tcagcttcaa gtgacgcacc
     8521 acctacaagg gcaccgtcaa cgtctgggca agccatgtat gaagcaacat tttcaggttt
     8581 aacagaacca ccgtattgaa cacgaacttt gtctgcgact tcttgaccaa agtcagcagc
     8641 tacaacgtca cgaacaactt tacacatttt ttgtgcatcg tcttgtgaag ctgatttacc
     8701 agtaccgata gcccagattg gctcataagc gataactgag gcagcaactt gttcagcagt
     8761 caatccagcc aatgcagcag atacttgagc acctacgaat tcagcagctt taccagcttc
     8821 gtaagtttca agtgattcac cacaacagat gattggaagc ataccgttcg caaagattgc
     8881 ttttgctttt ttgttgatat cttcgtcagt ttcatggaag tagtcacggc gttctgagtg
     8941 accgataaca acgtaatcag taccgatttc tttcaaaact tgtgggctag tttcaccagt
     9001 gaaagcacct gcattttcaa agtagcagtt ttgagcagca actttaaggt tcgagccttt
     9061 tgcaacagca agaacagttg tcaaatcaag agctggagca gcgatacctg cttcaacaag
     9121 atctgatgaa ggaagttttg atgcaactgc ttcaacgaat gctttagctt cttctggatt
     9181 tttgttcatt ttccagttac cagcgataaa tggtttacgt gacatttcac atacctcttt
     9241 tttcaattta ttttctattt attctatcac aattatacac agcttgcaaa tcttactcgg
     9301 atttcaagcc tacttgcatg gattaatcct tccagagatc catggcattt ctgaaatctg
     9361 cagatacctg tgtcaactga ggattgcttg cttctctttc tgctctcttg gcctcaattt
     9421 gagccacact cttgattcct tcatggcgcc agtttctcaa aattgcctga atgtacttcc
     9481 agtttggttt tccattcaaa acagcttcac gaagagcctc cttaatcaag tcagcactgg
     9541 ttccatcttc ctttagtgtc ttggtcaaat cctcaatctc aaaaggcgtc aacaatcgtc
     9601 ccaactcctg ctggaaggtt tccaccaaat ccttgagctg gttttgaggt gttagctggt
     9661 ctgaacttga acgaactgct ccaaacaggt catccaaacg ttccaaggcc aaactagcat
     9721 caaagaacaa ttcaatttcg ccatttaatt cgatagtacg atactggagc agtccccttt
     9781 ccgtcagatt agaaatggac tggttgacat ccgaaatttc cttgccaatc ctttcagcaa
     9841 tctggcttgg cgacatttct tctaagcctg tcgtattttg caaatagaaa aattgccaaa
     9901 ccagaaaatc gtcgctagaa ggaaagagtt ccttaaaatg caagagcagg gcactcggta
     9961 aaaccaaggt acctgattta aaagcgtcta aatatgtcat aattcctctt ataaataccc
    10021 atatgcagat gcatcatctt ctatctttct ccaatcaaaa ggcgtctcct gatagaccgc
    10081 atggtttacc caattactga aaaagagggc tgcagatgaa gaccaacaaa gacaaggtac
    10141 ctgattaaca tcatcatctt taaagtaatt ttctggaata tgtgggtcga aacctgcatc
    10201 acgatctcga aaatactctt ttgccaaggt atcacggtca tactccaaat gaccaaaact
    10261 ataaatttct cgtaaatcac gactggccaa aatagaaacc ccaacctgag gtccttctga
    10321 taaaatctcg agattggtct tgtttaagac ctcttcctta gaaatctccg tgtgccgtga
    10381 atgaggggat acatagctat catcaaagcc tctaaataga aggtgaccct cttttagggt
    10441 gtcctgagga taaatacctg atagcttact gtccatctgg tatttttcta caccatagcg
    10501 cagataaagc ccagcctgag ccccccaaca gatatgaagg gtcgaataga catgagtctt
    10561 agaccactca agcatctgtc taaattcctc ccaatagtcc acttcctcaa atggtaaatg
    10621 ctcaactgga gcacccgtga tgatcatccc atcaaaatac tcatccttga cttcaggaaa
    10681 agttttatag aaggtctcca tgtgctctga acgagttgtt ttagaacggt ggctctccat
    10741 atagagaaaa tcaatatcca gttgtagggg tgtattagcc aagtggcgca acaactgggt
    10801 ctctgtgacc attttctgtg gcatgagatt taaaattaaa atcttcaaag gacggatatc
    10861 ttggtgggca gcacgttgat catccatgac aaagatattc tctgtccgta aaatctcaac
    10921 agctggcaat tttttatcaa ttcgaatcgg cataaccctc tcctaactgt actctttcag
    10981 gaataattgc atatgtttga agacaaatct caaacactta gtccttttat tatactgcaa
    11041 gaagatatag ttttcaatta tagtttttct ctaactagtt atagtctatt tttatatcct
    11101 agtgtagaga aaacagccct agggactgtt ttcattaata atgcataaga actttgtagt
    11161 cgtagtcacc aattttttca cggccgttca attcatccaa ttcaacaagg aaggcacaac
    11221 ctgccataac accaccaagt ttttcaatca tctcgatagt tgccttaaca gttccacctg
    11281 tcgccaaaag gtcatctaca ataagaacac gttgacctgg cttaatggca tccgcgtgca
    11341 tagtcaaggt atcgacaccg tactcttttt catagtcagc agaaataact tcgcgtggca
    11401 atttacctgg cttacgaaca ggcgcaaaac caattcccaa ctcaaaggca actggacaac
    11461 ccacgataaa tccacgagct tcaggtccca cgatcatgtc gactttcttg tcagtagcat
    11521 actgaacgat ttcacgaaca gcgtagctat aagcatttcc atcagccatc aaaggactaa
    11581 tatcacggaa ggtaatgcct tcctttggat aattttcaat tgttgcaatg taatctttta
    11641 aattcatctt tttctttctt tcaaagtttt ttactctcta ttatagcata ttttttaaga
    11701 aagaaaaaag gaaaagttaa cttcaataat tatctaacgt tttgacgatt tataactagc
    11761 catcgcaata aagcccaatt tctgtttatt cttagcaaac attttataca tagttaaaaa
    11821 ctgctttcta ttctcctttt tacaagcatt tacacaaatt ttcaaagttc ctagcaaacc
    11881 ttcgtcataa atcatacccg ataatttcat taatgtcatt tcaccagtca atgctttcac
    11941 atcacaataa cctgattcta tcatcacctg ttcccaacca tcttgagtta aaggacctac
    12001 atttacatga attgcttgtg ataattcctg tctgatagac tctttagctt ccttaagaag
    12061 cacatcatgt gtcaagagaa gacctccagg ttttaatacc cttagatatt ccattacaca
    12121 ttttttctta gcttgatcgg cttgcatagt cagcatagct tcatttataa caatatcaaa
    12181 actagcatct tgataaggaa gtttcattgc atttgctctt tcaaaactga ttaaatgagc
    12241 aacacctgcc gttccagcaa attttttagc cacttctaaa gcttgagcat ccatatcaac
    12301 agcagttatc ttgcaaccaa aacgctgtgc caactcaatt gctgtagttc ccctattaca
    12361 cgcaacctct agtattctct tttcttttga aaatcctcct tctgcaatta accaatctgt
    12421 ggcacgcttt ccacctggac gtaagcgttt tttccccaat tttgctaaaa acttatgacc
    12481 tgcttctgac atttgaacac ctctgttttt tcttcattat aacacaaaat g
//
LOCUS       DAVMHG010000050        12275 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMHG010000050 DAVMHG010000000
VERSION     DAVMHG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.050"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            34..480
                     /gene="argR"
                     /locus_tag="VYR08_001127"
     CDS             34..480
                     /gene="argR"
                     /locus_tag="VYR08_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3466664.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            531..3065
                     /gene="mutS"
                     /locus_tag="VYR08_001128"
     CDS             531..3065
                     /gene="mutS"
                     /locus_tag="VYR08_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV3466665.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3212..4906
                     /gene="patA"
                     /locus_tag="VYR08_001129"
     CDS             3212..4906
                     /gene="patA"
                     /locus_tag="VYR08_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV3466666.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5236..5499)
                     /locus_tag="VYR08_001130"
                     /pseudo
     CDS             complement(5236..5499)
                     /locus_tag="VYR08_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5697..7463
                     /gene="patB"
                     /locus_tag="VYR08_001131"
     CDS             5697..7463
                     /gene="patB"
                     /locus_tag="VYR08_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV3466667.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7602..8291
                     /locus_tag="VYR08_001132"
     CDS             7602..8291
                     /locus_tag="VYR08_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV3466668.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            8295..8474
                     /locus_tag="VYR08_001133"
     CDS             8295..8474
                     /locus_tag="VYR08_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466669.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            8461..9810
                     /locus_tag="VYR08_001134"
     CDS             8461..9810
                     /locus_tag="VYR08_001134"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV3466670.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            9934..11394
                     /locus_tag="VYR08_001135"
     CDS             9934..11394
                     /locus_tag="VYR08_001135"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV3466671.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            11408..11842
                     /locus_tag="VYR08_001136"
     CDS             11408..11842
                     /locus_tag="VYR08_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3466672.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            11966..12037
                     /locus_tag="VYR08_001137"
     tRNA            11966..12037
                     /locus_tag="VYR08_001137"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11999..12001,aa:Glu,seq:ttc)
BASE COUNT         3670 a         2344 c         2796 g         3465 t
ORIGIN      
        1 atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg tcatcagtta
       61 ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca agatcggttg
      121 gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg cgaaatcggc
      181 ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga gacagaaaag
      241 attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc agagtttacc
      301 ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt agatgtaaac
      361 aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt tatttgtcga
      421 gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa agataagtaa
      481 ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa atggcgatag
      541 aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat ccagatgctt
      601 ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg gtcaatgctg
      661 cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat ccgatcccta
      721 tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt gagcagggtt
      781 ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg gttgttaaac
      841 gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag ccggacagtc
      901 agaataattt tttggtttcc atagaccgcg aaggcaatca atttggccta gcttatatgg
      961 atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg gtttgtgggg
     1021 aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct gaggaagaag
     1081 aacaaatcct cagccgccag atgaatctgg tactctctta tgaaaaagaa agctttgaag
     1141 accttcattt attggatttg cgattggcaa cggtggagca aacggcatct agtaagctgc
     1201 tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct gttatccgct
     1261 acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg gatttggttg
     1321 agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat gaaaccaaaa
     1381 cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt gataaggaac
     1441 gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt gagcgtagtg
     1501 acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt cgtgtttctt
     1561 ttggtaaaac caatccaaag gatctcttgc agttggcgac taccttgtct agtgtgccac
     1621 ggattcgtgc gattttagaa gggatggagc aacctactct agcctatctc atcgcacaac
     1681 tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct gaagctcctc
     1741 atgtgattac agatggggga attatccgga ctggatttga tgagacttta gacaagtatc
     1801 gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag gagcgagaaa
     1861 actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac tattttcatg
     1921 tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg acgctgaaaa
     1981 actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg cttgaggcgc
     2041 gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa gaggtcggca
     2101 agtacatcca gcgtttacaa gctctagccc aaggaattgc gacggttgat gtcttacaga
     2161 gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt gacgattcac
     2221 aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg gctcagacct
     2281 atattccaaa tacgattcag atggcagaag ataccagtat tcaactggtt acagggccaa
     2341 acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt atggcccagc
     2401 tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg atttttaccc
     2461 gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg gagatgatgg
     2521 aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt gatgaattgg
     2581 gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc gaatatatcc
     2641 atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg actagtctgg
     2701 agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag gatgggcagg
     2761 tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt atccatgttg
     2821 ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt ttgactcagc
     2881 tagagaatca aggaacagag agtcctcctc ccatgagaca aactagtgct gtcactgaac
     2941 agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta gctaaactgg
     3001 atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta aaacagaaac
     3061 tataaaacca agactcacta gttaatctag ctgtatcaag gagacttctt tgacaattct
     3121 ccactttttt gctagaataa catcacacaa acagaatgaa aaggagctga cgcattgtcg
     3181 ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa aaacctacaa
     3241 gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt cacttctgca
     3301 accgcgttat ctgcaggaag tcttaggcgc cctccttgct gggaaatatg aagctattta
     3361 tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg gtggactcaa
     3421 tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg aggatgcctt
     3481 ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg gaaatctagt
     3541 cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga ccttccagat
     3601 tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc aaaccttacc
     3661 ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga ctgctgtcat
     3721 gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc gcatcaatgc
     3781 cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc aagaaaaaga
     3841 gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc tttacattgg
     3901 ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg cggtcttcct
     3961 ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg gttccatcgc
     4021 ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg gatttttggg
     4081 aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc ttgacgcaga
     4141 gccagctatg accttcaagg atatcccaga tgaagagttg gttggaagcc ttagctttga
     4201 aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg tgagctttac
     4261 tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa agtcaacctt
     4321 ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa tcggaggtaa
     4381 ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg ttctccaacg
     4441 tgccattctt tttagtggaa cgattgcaga taatttgaga caggggaagg gaaatgctac
     4501 tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca ttcatcgtat
     4561 ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg gtggacaaaa
     4621 acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga tttttgatga
     4681 ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt tgaataagga
     4741 cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg tccatgcaga
     4801 caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg cagacttggt
     4861 tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaatgaaaga caaactataa
     4921 gaaaagtcaa tagttttatc taaactattt cttatttcaa tttgatgatt tggcgatgat
     4981 tttagagcac ggcaaaaagc ccttgaaaaa gtccattttt tcaaaggtaa tcctgtgtta
     5041 atttcagaaa ttacatcact ttttgttcgt caaatggcag ctctttttta ggatataaaa
     5101 cagggttcgg ataagttttt ttgcaaggtg gatgatggct acattgtaat gttttccttg
     5161 ttctaactta gtcttaagat aggccttaaa agcaggtgaa aagcgagggc atgctttggc
     5221 agcttgtatg agtacctacc gcagatgagg ggaaccccgt ttgaccattc ttcctgctaa
     5281 atcaatctga cctgactgat aaatagaaga atccagtcca gcgaaagctt gtaattgagc
     5341 aggattatca aaggcatgaa tatttcgaat ctcggctaaa atgaccgccc ctaaacgatt
     5401 ctcaatccca gtaaccgtcg tgatgaccga gttgaactca gccatcaagt cattgacaca
     5461 ttttttcgcc ttgtcaatga gcctcttgta atgtttgatg ttttcattac aagagataaa
     5521 acgtttatgc gttatcaaac tcattaccaa ttgaaacaaa aagctgtggt tagatccttt
     5581 cggaaattgt caagcgattg gaggaaatga actaatccac agcggcttat tccaagtata
     5641 ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg agtaaaatga
     5701 agacagttca atttttttgg cattatttta aggtctacaa gttctcattt gtagttgtca
     5761 tcctgatgat tgttctggcg acttttgccc aagccctctt tccagtcttt tctggacaag
     5821 cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca gaacttgtat
     5881 ggcaaagcct atcaggaatc atggtcaatc ttggcctgct ggttttggtt ctatttatct
     5941 ctagtgtaat atacatgtgt ctcatgacgc gcgtgattgc agaatcgacc aacgagatgc
     6001 gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac cgtcgacaag
     6061 atggcgatat cctgtctcat tttaccagtg atttggataa tatcctccaa gcctttaacg
     6121 aaagcttgat tcaggtcatg agcaatattg ttttatacat tggtctgatt cttgtcatgt
     6181 tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg gctttcctta
     6241 tgctgatttt catcgtgaaa atggcacgta aatacaccaa cctccagcag aaagaggtag
     6301 ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg attgtgcaag
     6361 gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg cgcaaggcaa
     6421 cctttaaagg aagaatgttc tcaggaattc ttttccctgt catgaatggg atgagcctga
     6481 ttaatacagc catcgtcatc tttgctggtt cggctgtact tttgaatgat aagtctattg
     6541 aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag cagtactacc
     6601 agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt actggagctg
     6661 aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag gctccaacct
     6721 tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac ttgcctgata
     6781 aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca gcagttgttg
     6841 ggcctacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt tatgatgttg
     6901 atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta gatagtctta
     6961 gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg attcgagaca
     7021 atatccgatt tggtgtgcca gatgctaatc aggaaatggt tgaggtagca gcaaaagcaa
     7081 cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt attgatgatg
     7141 accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga accctgatga
     7201 cagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg gtgacggaaa
     7261 gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc gtcattgccc
     7321 accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat ggagaagtca
     7381 ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca gaactctatc
     7441 acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct ttttcttgtc
     7501 cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc attttgagtg
     7561 atatgatagg actatcgtta gtattcgaaa ggagaggcat catggctaga acggttgtag
     7621 gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat tcatctgtat
     7681 cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc attcctgttg
     7741 gtgatgagtc agttgtacgt gattatgtgg aaatgattga caaactcatt ttgacaggag
     7801 gccaaaatgt tcatcctcag ttttatggag agaaaaagac cgtcgaaagc gatgattaca
     7861 atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt cagaataaac
     7921 caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt ggaaccctca
     7981 atcaagaaat cgaaggtcac tggcaaggac tacctttcgg gacatctcac tctattgaga
     8041 cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc aactcagtcc
     8101 atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt gattcaagag
     8161 accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt ttacagtggc
     8221 atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag tatttattga
     8281 atgaactgta acgattggaa catctggtcg ttctttcttt tattttttca aaatttttag
     8341 aatgtggcat tttacgcaaa cgtttgaatt ctgataaaaa ttggagaaat tcgacaaaaa
     8401 aacttgaaaa aaacgaaggt aagcgttatg atagaaaaga agaaatattg gaggaagaac
     8461 atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc accacatgaa
     8521 gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa aggaactggt
     8581 gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgaccg cgaagaattt
     8641 gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt ggttgtgatc
     8701 ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa tcaccatttt
     8761 gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc aggaaactca
     8821 atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga cttctcagta
     8881 aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg tgtctttaaa
     8941 gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta tgcaacaact
     9001 gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg aacatttgtt
     9061 gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt gcttccaatc
     9121 gcagcatcag gagctgacat caaagctctt atggaaggtg cgaatgcagc tcgcaaagac
     9181 tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagtcgt tcgtaacatc
     9241 ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc acttcaatac
     9301 ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca aaaaggtatc
     9361 tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt tatccaagaa
     9421 ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa aaacgtgctt
     9481 attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa agacgttgac
     9541 tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg tgatgtacca
     9601 aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac tatctacttc
     9661 ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt tgaccaacca
     9721 ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg atttgaagaa
     9781 ttgagcaaag aacttaacgc acgtctataa tagaagaaaa gagtggtttg cccactcttt
     9841 ttactctctt tatccataga aattggactc agccaagact tgtgatataa tatagaaagc
     9901 aaaaaggcag acgcctagat aataggagaa actatgtcaa aagatatccg cgtacgttac
     9961 gcaccaagtc caacaggact actacacatc ggaaatgccc gtacagcatt gtttaactac
    10021 ttgtatgcgc gccatcatgg tggaacattt ctcatccgta tcgaagatac tgaccgtaaa
    10081 cgccatgtcg aggatggtga acgttcacaa cttgaaaacc ttcgctggtt aggcatggat
    10141 tgggatgaaa gtccagaatc acatgagaat tatcgccagt ctgagcgttt ggacttgtat
    10201 caaaaataca ttgaccaact attagctgaa ggaaaagcct ataaatctta cgttacagaa
    10261 gaagagttgg cagctgaacg tgaacgtcaa gaagtagctg gcgaaacacc acgctacatc
    10321 aatgaatacc ttggtatgag tgaagaagaa aaagcggctt atatcgcaga acgtgaagca
    10381 gctggtatca taccaacagt tcgtttggct gtcaatgagt caggtatcta caagtggcat
    10441 gatatggtca aaggtgatat cgaatttgaa ggtggcaata tcggcggtga ctgggttatc
    10501 caaaagaaag acggttaccc aacttacaac tttgccgttg ttatcgatga ccacgatatg
    10561 caaatctctc atgttatccg tggagatgac catattgcta atacaccaaa acagcttatg
    10621 gtctatgaag ctcttggttg ggaagctcca gagtttggtc acatgacctt gattatcaac
    10681 tctgagactg gtaagaagtt atctaaacgt gatactaata cacttcaatt tatcgaagat
    10741 taccgaaaga aaggttacct tccagaagca gtctttaact ttattgctct tcttggttgg
    10801 aacccaggtg gtgaagatga gattttctct cgtgaagaac tcattaaact ttttgatgaa
    10861 aaccgcctca gcaagtcacc agcagccttt gatcagaaaa aattagactg gatgagcaat
    10921 gattatatca agaatgcaga ccttgaaacc atctttgaaa tggcaaaacc attcttagag
    10981 gaagcaggcc gtttgactga caaggctgaa aaattagttg agctctataa accacaaatg
    11041 aaatcagtag atgagattat cccattgaca gatcttttct tctcagattt cccagaattg
    11101 acagaagcag agcgcgaagt catgacgggt gaaacagttc caacagttct tgaagcattc
    11161 aaagcaaaac ttgaagcgat gacagatgat gaatttgtga cagaaaatat cttcccacaa
    11221 attaaagcag tccaaaaaga aacaggtatt aaagggaaaa atcttttcat gcctattcgt
    11281 atcgcagttt caggcgaaat gcatgggcca gaattaccag atacaatttt cttgcttgga
    11341 cgtgaaaaat caattcagca tatcgaaaac attctaaaag aaatctctaa ataaagagga
    11401 tacaataatg gacatctggg aaaagatgta cgaagaagca cagaaactat acaatccaca
    11461 tgaagtatca gactttgttt atgccaatca tgttgtagct gcagtagaag caggagatgg
    11521 acaaatattt acaggattct gtatggaggg aacctgtggt gttttccatc tctgcgcaga
    11581 acgggcgacc ctcttcaata tgtaccaatt ttcaggacaa actaaggtta agaaagtatt
    11641 agcctttcga gacaaaccac cttatggtgg aagttcagcc atgccttgcg gtgcttgtag
    11701 agaattcctt ttagagctta atgctgaaaa taaagatgca gaattcatga tggactataa
    11761 tataagaaaa acagttaaag tcgcagaact aatcccttat tggtggggag aagaacgtgc
    11821 ttctaagttt aatgagcgat agaagagtca gtataattct ggctcttttt actataagtt
    11881 gcaaaaaaaa tgaaaaaaat tagaaaaagt agttgacaaa gtttgaaaag actgtataat
    11941 agtaagagtt gaaaataaca actcaggtcc gttggtcaag gggttaagac accgcctttt
    12001 cacggcggta acacgggttc gaatcccgta cggactatgg tatgttgcgg ttggaacact
    12061 tgatgaaaaa aagtttaaaa aaacttaaaa atcttcaaaa aagtgttgac aagtgaaagt
    12121 ggctgtgata tactaatata gttgtcgctt gagagaagca agtgacaaag acctttgaaa
    12181 actgaacaag acgaaccaat gtgcagggcg ctacaacata agttgtagta ctgaacaatg
    12241 aaaaaacaat aaatctgtca gtgacagaaa tgagt
//
LOCUS       DAVMHG010000051        12108 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMHG010000051 DAVMHG010000000
VERSION     DAVMHG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.051"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            96..1121
                     /gene="trpS"
                     /locus_tag="VYR08_001138"
     CDS             96..1121
                     /gene="trpS"
                     /locus_tag="VYR08_001138"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV3466673.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1273..2751
                     /gene="guaB"
                     /locus_tag="VYR08_001139"
     CDS             1273..2751
                     /gene="guaB"
                     /locus_tag="VYR08_001139"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV3466674.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2802..3899)
                     /gene="recF"
                     /locus_tag="VYR08_001140"
     CDS             complement(2802..3899)
                     /gene="recF"
                     /locus_tag="VYR08_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV3466675.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3902..4270)
                     /gene="yaaA"
                     /locus_tag="VYR08_001141"
     CDS             complement(3902..4270)
                     /gene="yaaA"
                     /locus_tag="VYR08_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV3466676.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQASRFPGR"
     gene            4429..5679
                     /locus_tag="VYR08_001142"
     CDS             4429..5679
                     /locus_tag="VYR08_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3466677.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5676..6959
                     /locus_tag="VYR08_001143"
     CDS             5676..6959
                     /locus_tag="VYR08_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV3466678.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7001..7822
                     /locus_tag="VYR08_001144"
     CDS             7001..7822
                     /locus_tag="VYR08_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3466679.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7833..8378
                     /gene="pgsA"
                     /locus_tag="VYR08_001145"
     CDS             7833..8378
                     /gene="pgsA"
                     /locus_tag="VYR08_001145"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV3466680.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8375..9202
                     /locus_tag="VYR08_001146"
     CDS             8375..9202
                     /locus_tag="VYR08_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3466681.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9187..10026
                     /locus_tag="VYR08_001147"
     CDS             9187..10026
                     /locus_tag="VYR08_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV3466682.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQFLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10019..10813
                     /locus_tag="VYR08_001148"
     CDS             10019..10813
                     /locus_tag="VYR08_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3466683.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10872..11690
                     /gene="mreC"
                     /locus_tag="VYR08_001149"
     CDS             10872..11690
                     /gene="mreC"
                     /locus_tag="VYR08_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV3466684.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11690..>12108
                     /gene="mreD"
                     /locus_tag="VYR08_001150"
     CDS             11690..>12108
                     /gene="mreD"
                     /locus_tag="VYR08_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV3466685.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVY"
BASE COUNT         3681 a         1832 c         2595 g         4000 t
ORIGIN      
        1 atctgtaaaa agcaaggctg atttattttg ataaattacg gttatttcat taaaaaaatg
       61 ctataattga aaggactata tcgaaggaga acaaaatgac taaacccatt attttaacag
      121 gagaccgtcc aacaggaaaa ttgcatattg gacattatgt tggaagtctc aaaaatcgag
      181 tattattaca ggaagaggat aagtatgata tgtttgtgtt cttggctgac caacaagcct
      241 tgacagatca tgccaaagat cctcaaacca ttgtagagtc tatcggaaat gtggctttgg
      301 attatcttgc agttggattg gatccaaata agtcaactat ttttattcaa agccagattc
      361 cagagttggc tgagttgtct atgtattata tgaatctagt ttcgttagca cgtttggagc
      421 gaaatccaac agtcaagaca gagatttctc agaaaggatt tggagaaagc attccgacag
      481 gattcttggt ctatccaatc gctcaagcag ctgatatcac agctttcaag gctaattatg
      541 ttcctgttgg gacagatcag aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt
      601 ttaacaatgc atataactgt gatgtcttgg tagagccgga aggtatttat ccagaaaatg
      661 agagagcagg gcgtttgcct ggtttagatg gaaatgctaa aatgtctaaa tcactaaata
      721 atggtattta tttagctgat gatgcggata ctttgcgtaa aaaagtaatg agtatgtata
      781 cagatccaga tcatatccgc gttgaggatc caggtaagat tgagggaaat atggttttcc
      841 attatctaga tgtttttggt cgtccagaag atgctcaaga aattgctgat atgaaagaac
      901 gttatcaacg aggtggtctt ggtgatgtga agaccaagcg ttatctactt gaaatattag
      961 aacgtgaact gggtcctatt cgtgagcgcc gtattgaatt tgctaaggat atgggagaag
     1021 tttataatat gattcaaaaa ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat
     1081 ctgaggtaaa aggggcaatg ggacttcatt actttaacta agtttatttt acaagaaact
     1141 atcttttcta tctcgaagaa aagatagttt tttatttacc cctgtaacat ttgacaaatt
     1201 tttatagaat ggtatgatag atacaatata aaaagagtca agctcaaaaa gaaagaaaag
     1261 aggaaacttc gaatgtctaa ttgggacact aaatttttga aaaaaggttt tacctttgat
     1321 gatgtattgc ttattccagc tgaaagtcat gtgttgccta acgatgcaga tttaacaact
     1381 aaattggcag ataatttgac tttaaatatc ccaattatta ccgctgccat ggacacagtt
     1441 acagagagtc aaatggccat tgctattgct cgtgcaggcg gtctcggagt tatccataaa
     1501 aacatgtcaa ttgctcaaca agcagacgag gttcgtaagg taaaacgttc tgaaaatgga
     1561 gttattattg atccgttctt cttgacgcct gaacatacaa ttgctgaagc agatgagctt
     1621 atgggtcgtt accgcatcag tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg
     1681 gttggtattt tgacaaaccg agatcttcgt tttatttcag attataatca accaatttca
     1741 aaccatatga ctagtgaaaa tcttgttact gctcctgtgg gtacggatct tgcaacggct
     1801 gagagtattc ttcaagagca tcgtattgaa aaacttccgt tggtcgatga agaaggcagt
     1861 ctttctggtt tgatcactat caaagatatt gaaaaagtta ttgagtttcc aaatgcagct
     1921 aaagatgagt ttggtcgtct tctagttgca ggtgcagtag gtgttacttc agatacattt
     1981 gaacgtgcag aggctctttt tgaggcagga gcggatgcga ttgttattga tactgcacat
     2041 ggtcattctg caggtgtctt gcgtaaaatt gccgagattc gtgctcattt cccagatcgg
     2101 actttgattg ctggaaatat tgctactgct gaaggtgcac gtgcccttta tgaagcgggt
     2161 gtagacgttg ttaaggttgg tattggacca ggttctatct gtactactcg tgtgattgct
     2221 ggtgttggtg ttccgcaagt aacagctatc tacgatgctg cagctgttgc gcgcgaatat
     2281 ggtaaaacga ttattgctga cggtgggatc aagtattctg gagatattgt aaaagcactt
     2341 gctgcaggtg gaaatgctgt tatgcttgga tctatgtttg ctggaactga tgaagctcca
     2401 ggcgaaactg aaatcttcca aggacgtaaa ttcaagactt accgtggtat gggatcaatt
     2461 gctgctatga agaaaggttc aagcgaccgt tatttccaag gttctgtcaa tgaagcaaac
     2521 aagcttgttc cagaaggaat tgaaggtcgt gttgcttata aaggagcggc agctgatatt
     2581 gttttccaaa tgattggtgg tattcgctct ggtatgggtt actgtggtgc agctaacctt
     2641 aaagaactac acgataatgc tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc
     2701 catcctcatg atgtgcaaat tactaatgag gcaccaaatt attctatgta aaaaacaatg
     2761 aaaagaactc cagtgaaaac aggagttctt ttacaatgtt gtcaatttcc atttacagca
     2821 gctttaccat cctgaatagt gaagatactt agattttctg gcagattttg aagatggtct
     2881 aagcttgttg ttgtgataaa ggtttggatt gattgagaaa tcgtttctaa taattttaac
     2941 tgtctagtgt tgtcaagttc actcatcaca tcgtcaagca gtaatatagg agattctgtg
     3001 gtaatgcttt ccattaattc gatttctgct aattttatcg agaggacgag actacgatgt
     3061 tgaccttggc ttccgaaact agcatccatc ccatttatat aaaaagaaat gtcatctcga
     3121 tgaggaccga caccagtatt ctttttaaat aaatctctgg atctactttt ttctaaagca
     3181 attttgaaag attcggataa gttttgtttg tcagttatat tgacagaaga ttgataggat
     3241 attgacaact cttcgatctg attagagagt tcaaaatgtt tcttacgccc aaatgattct
     3301 agttttttta tgaaatctaa gcggtgattc attacacgac atccataatc aactagctga
     3361 tcatctaaca cagaaaggaa tgtttcatct attttttgag ctgattttag gtaagtgttt
     3421 ctttgcttta ggatgtggtt ataattggtt aagtcagata aatagattgg cttaatttgc
     3481 ccaagttcca tatcaatgaa ttttcgtcga atcgaaggtg ctcctttaat tagttgtaaa
     3541 tcttcaggag caaataagac aacattcatg tgtcctacat aatctgaaag gcgtgcctgt
     3601 tttaagtgat taacttttgt tacacgacct ttttgtgtta gttcgatttc taggggaatg
     3661 gatccagttt ttttctgaac gagacctgaa agatgaagtt gttcctcatc aaaatgaatg
     3721 agatttttat ctgttcgagt tcgatgacta cgcgttaagg ctaaaaaata gatagcctct
     3781 aacatgtttg tttttccttg tgcattacgt cccaaaaaga cattcaattt aggattaaag
     3841 tctattttcg tttctttgta attacgaaaa gtcttgagag atagatgttg tagccacatg
     3901 attatctacc agggaatcgt gaagcttgtt ggcttttagg tgatgaagca ggtttcgatt
     3961 tgtctttttt aactccctta ttcatctttt tgacaagttt agcgatccgt tctttttcaa
     4021 ctttatcagc ttggtattca tcttgttctt cagaagtagg ttgtgtcaac aagatgtcaa
     4081 tgttcatgtc agggatgtca actttatcgc caatacgaag tttttttcca cgacggctct
     4141 ctaattccct attaaagtaa acagaatgtt cagagagaaa tgatttaata gctcctccgc
     4201 tatgtgtaat tccaagttct ttgaatagtg cttggagggt aataaattct tcaaataatt
     4261 tgtattccat aattcacctc aatctttggt attataccat attttcctaa aaatagtgag
     4321 agagacagcg agaaatgtaa gcgattttta tttcaaaagt gttacaggga acaagaaaat
     4381 ttcaggtttt cgtgatataa tagaagtctg tatataagga ggtaaatcat ggagttagtg
     4441 catggaattt caacacattt tatccaatca aaaaagttta aaacaaacaa aattaccgtg
     4501 cgttttaccg ctccattatc ccttgatacg attgcaggtc acatgttgag tgcaagtatg
     4561 ctagagactg ctaatcagat gtaccccact tctcaagatt tgaggagaca cttggccagt
     4621 ctatacggta cagatatgtc aaccaattgt ttcagaagag ggcaaagcca cattatagaa
     4681 ttgacattta cctatgttcg tgatgagttt ttaagtagga aaaacgtgct aacctctcag
     4741 attttggaac ttgtaaaaga aactcttttt tcacccgtag tagttgataa tgggtttgat
     4801 ccggccttat ttgaaattga gaaaaaacaa ttgctagcaa gtttagcagc tgatatggat
     4861 gattcttttt attttgcaca taaagaattg gataaattgt tttttcatga tgaacgtctt
     4921 caattggaat atagtgattt acgaaatcgt attttagctg aaactccaca aagttcttat
     4981 tcttgtttcc aagaattttt agccaatgat cgaatagatt tctttttcct aggtgatttt
     5041 aatgaggttg aaattcaaaa tgtattagaa tcatttggct ttaaaggtcg aaaaggagat
     5101 gtgaaggttc agtattgtca accttattct aatatccttc aggaaggtat ggttcggaaa
     5161 aatgtgggac aatccatttt ggaattaggt tatcattact gttctaaata tggtgatgag
     5221 caacatttac ccatgattgt aatgaatggt ttacttggtg gatttgctca ctctaagctc
     5281 tttacaaatg tccgtgaaaa tgctggatta gcttatacca tttcaagtga gcttgattta
     5341 tttagtggat tcttgaggat gtatgctggt atcaatcgag aaaatcgtaa ccaggctcgt
     5401 aaaatgatga ataatcaact gcttgattta aaaaaaggtt attttacaga gtttgagtta
     5461 aatcagacca aggaaatgat tcgttggtcg ttgttacttt ctcaagataa tcaatcttca
     5521 ttgattgaac gtgcttatca aaatgcctta tttggaaaat cttcagcaga ctttaaaagt
     5581 tggattgcaa agcttgaaca aattgacaaa gatgctattt gtagagtagc taataatgtg
     5641 aaactacaag cgatttactt tatggaagga atagaatgac aaaggttgtt tttgaagaaa
     5701 aatactatcc agctgtaaaa gaaatggttt atcgaactcg tttggccaac ggattgacag
     5761 ttgctctttt gcctaaaaag gaatttaaag aggtttacgg gagtgtcact gtacagtttg
     5821 gttcggtaga tacgtttgtc acagaagttg acggagatgt aaaacaatat cctggaggaa
     5881 ttgctcattt tcttgaacat aaattatttg agagagaaga ttctagtgat ttgatgtcgg
     5941 cttttacgag tctaggtgca gatagtaatg cctttacaag ctttacaaaa acaaactatc
     6001 ttttttcagc aacggattat tttttagaaa atttagattt acttgatgaa ttggtaacat
     6061 cagcacactt tactgaagct tccattctga cagagcagga tattattcag caagaacgag
     6121 aaatgtacca agatgatcca gattcgtgtt tattcttttc aactttagcg aatttgtatc
     6181 ctggtacacc tttagcaact gatatagttg gaagtgagga gtccatttcc caaatcaatc
     6241 taactaattt gcaagaaaat tttacaaagt tttacaaacc tgtaaacatg tctctgtttt
     6301 tagttggtaa ttttgatgtg gagcgagtac aggactattt tgaaagcaaa gaactgaaag
     6361 attcagattt tcaggaagta gcaagagaaa agttgttttt acagcctgta aagccaacag
     6421 atagtatgag aatggaagta tcttctccca aactagcgat tggagttaga ggtaagcgag
     6481 aagtttctga agcggattgc tatcgacatc atattttatt aaaattattg tttgcaatga
     6541 tgtttggttg gacttcggat cgttttcaaa aatgttatga atcaggtaaa attgatgcgt
     6601 ccttatctct ggaagttgaa ataacaagtc gctttcattt tgtcatgttg acaatggata
     6661 cgaaagagcc agttgctttg tctcatcaat ttaggaaggc tattcgtaat tttacaaagg
     6721 atttagatat tacagaggaa catttagata ttatcaaaag agagatgttt ggcgaatttt
     6781 tcagtagcat gaactctctt gaatttattg caacgcaata tgatgctttt gaaaatggtg
     6841 agacaatttt tgatttgccg aaaattttac aggaaattac tttagaggat gtccttgatg
     6901 ctggacatca tttaatagat gatggtgaca tagttgattt tacaatattc ccatcgtagt
     6961 aacctatcat aatagacact agaaagaagg gatgacaagt atgagaaaaa aaacaattgg
     7021 agaggtttta cgattagcta gaatcaatca gggattgagt ttagatgaat tgcagaaaaa
     7081 gacagaaatc cagttagata tgttggaagc aatggaagca gacgatttcg atcaacttcc
     7141 aagtcctttt tacacgcgtt ctttcttgaa aaaatatgca tgggctgttg agttagatga
     7201 ccaaattgtt ttggatgctt atgattctgg gagtatgatt acttatgagg aagtagatgt
     7261 tgatgaagat gagttgacag gtcgtagacg ttcaagtaag aaaaagaaga aaaaaacatc
     7321 atttttacct ttattttatt ttatcctttt tgctttatcg attttaattt ttgtgactta
     7381 ttatgtttgg aactatattc aaactcaacc agaggagcct tctctttcta attacagtgt
     7441 ggttcaatca acaagttcaa ctagctctgt tccccactcc tcaagtagta gttcttctag
     7501 tatagaatca gctataagtg tatcaggcga aggaaatcat gtagaaatcg cttataagac
     7561 aagtaaggaa acagttaaat tgcaattggc agtttcagat gttacaagtt gggtcagtgt
     7621 ttcagaaagc gaacttgagg gcggtgtaac cttatcgcca aagaagaaaa gtgcagaagc
     7681 aacagttgca actaaaagtc ctgtaacaat tacgttaggt gttgtaaaag gtgttgattt
     7741 gacagtagat aatcagactg ttgatttatc gaaattaaca gctcagactg gacaaatcac
     7801 tgtaaccttt actaaaaatt aaggaaaaac gaatgaaaaa agaacaaatt cccaatctct
     7861 taacaatagg tcgaattctc tttataccta tttttatctt tattttaacg ataggaaatt
     7921 cgatagagag tcatatagtt gcagctatta tctttgctgt tgccagtatt accgactatt
     7981 tagatggata tttagctcgt aaatggaatg tggtcagtaa ttttggtaaa tttgcagatc
     8041 ctatggcgga taagttacta gttatgtcgg cttttattat gttgattgag ttaggtatgg
     8101 ctccggcttg gattgttgca gtgattatct gtcgtgagtt agctgtgaca ggtttaaggc
     8161 ttttattggt tgaaactggt ggaacaattt tagcagcagc aatgcctgga aaaattaaaa
     8221 cttttagtca gatgtttgcc attattttct tgctattaca ttggactttg cttggtcaag
     8281 ttctacttta tgtagcctta tttttcacta tctactctgg ctatgactat ttcaagggta
     8341 gtgcctatgt atttaaaggg acatttggtt cgaaatgaaa tcaataattg atgtaaaaaa
     8401 tctttctttt cgctataaag aaaatcagaa ctactacgat gtgaaggata ttacgtttca
     8461 cgtgaaacgt ggagaatggc tttcgattgt agggcataat ggtagtggta aatcaacgac
     8521 ggttcgatta attgatggct tactggaagc agaatccgga gagattgtaa ttgatggcca
     8581 acgtttgact gaggaaaatg tttggaatat acgtcgtcaa atcggtatgg tttttcaaaa
     8641 tccagacaat caatttgttg gagcgactgt tgaagatgat gttgcttttg gtttggaaaa
     8701 tcagggactt tctcgtcaag aaatgaaaaa gagagtagaa gaagctctgg ctttagttgg
     8761 catgttggac tttaaaaaga gagagccagc gcgtctatca ggtggccaaa agcaacgtgt
     8821 ggccattgca ggtgttgtag ccctaagacc agctatttta atcttagatg aagcaacgag
     8881 tatgttggat cctgaggggc gtagagaact tattgggaca gtaaaaggaa ttcgaaaaga
     8941 ctatgatatg acggtcattt ctattaccca tgatttggaa gaagtcgcca tgagtgatcg
     9001 cgtattggtc atgaaaaaag gggaaattga atcaactagt agtccaaggg agcttttctc
     9061 tcgaaatgat ttagatcaaa ttggattaga cgatcctttt gctaatcaat taaaaaaatc
     9121 tttgagccag aatggctatg atttacctga aaattatttg acagaaagtg agctagagga
     9181 taagctatgg gaattgctct agaaaatgtg aattttacat atcaagaagg tactccctta
     9241 gcttcagcag ctttgtcgga tgtttctttg acgattgaag atggctctta tacagcttta
     9301 attgggcaca caggtagtgg taaatcaact attttacaat tcttaaatgg tttattggtg
     9361 ccaagtcaag ggagtgtgag gatttttgat accttaatca cctcgacttc taaaaataaa
     9421 gatattcgtc aaattagaaa acaggttggc ttggtatttc agtttgctga aaatcagatt
     9481 tttgaagaaa cggttttgaa ggacgttgct tttggaccgc aaaattttgg agtttctgaa
     9541 gaagatgctg tgaagactgc gcgtgagaaa ctggctctgg ttggaattga tgaatcactt
     9601 tttgatcgta gtccgtttga gctgtcaggg ggacaaatga gacgtgttgc cattgcaggc
     9661 atacttgcca tggagccatc tatattagtc ttagatgagc caacagctgg tctagatcct
     9721 ctagggagaa aagagttgat gaccctgttc aaaaaactcc accagtcagg gatgaccatc
     9781 gtcttggtaa cgcatttgat ggatgatgtt gctgaatatg ctaatcaagt ctatgtaatg
     9841 gaaaagggac gtttagtaaa ggggggcaaa ccaagtgatg tctttcaaga cgttgttttt
     9901 atggaagaag ttcagttggg agtacctaaa attacggcct tttgtaaacg attggctgat
     9961 agaggcgtgt catttaaacg attaccgatt aagatagagg agttcaagga gtcgctaaat
    10021 ggatagtatg attttggggc gttatatccc aggggattcg attgttcacc gattggatcc
    10081 acgtagcaaa ttgcttgcta tgatgctact gattttgatt gtgttttggg ctaataaccc
    10141 cttgacgaat ctgattcttt ttatagcgac agggatattt attgccttgt caggagtatc
    10201 tctttcattt tttattcagg gcttgaagtc tatgtttttc ttgattgcct tcacaactat
    10261 ttttcaacta tttttcattt ctaatgggaa tgttttattt gagttttcgt ttgtgagaat
    10321 cacggattat gctttgcaac aagctgggat tattttttgt cgctttgtat tgattatttt
    10381 cttttcaact ttgttaacct taacaaccat gcctttaagt ttggcatcag ctgtcgaagc
    10441 tttattagca cctttaaagc gtgtgaaagt tccagttcat gaaattggat tgatgctgtc
    10501 tatgagtttg cgttttgtcc caaccttgat ggatgatacg acgcggatta tgaatgcaca
    10561 gaaagctcgt ggagtggatt ttggagaagg aagtatcgtt caaaaagtaa aggcgatgat
    10621 tcccattttg attcctcttt ttgcgacaag tttaaaacgt gcagattcct tggctatcgc
    10681 tatggaagcg cgtggctatc agggtggaaa aggcagaagt caataccgac aattgaaatg
    10741 gactctaaag gatacgctga ccattcttgt tattctcgta cttggttgtt gtttattttt
    10801 cttaaaatct tagtagcttt caggaattga taaaaagtta ctgtaacagt ttttgataga
    10861 aaggtaggga tatgaaccgt tttaaaaaat caaaatatgt cattattgtt tttgtcactg
    10921 ttctgcttgt gtcagctctc ttagcgacga cttattcaag tacaattgtg acaaaattag
    10981 gagatggaat ctcattggtt gatagagttg tacaaaaacc ttttcagtgg tttgattctg
    11041 tcaaatcaga tttggctcat ttgacacgaa catataatga aaatgaaagt ttgaagaaac
    11101 agctttacca attagaagtt aaatcaaatg aggtggaaag tttaaagaca gaaaatgaac
    11161 aactgcgcca attgcttgat atgaagtcta aattgcaagc cacaaagact ttagcagcag
    11221 atgttattat gcgttctccg gtatcttgga agcaggagtt gaccttagat gcaggtagat
    11281 caaaaggtgc ttctgagaac atgttagcta ttgcaaatgg tggcttgatt gggagtgttt
    11341 caaaagtaga ggagaactct actatagtca accttctgac aaatacggaa aatgctgata
    11401 agatttctgt taaaattcaa catggctcta ctacaattta tggaattatt attggctatg
    11461 acaaggaaaa tgacgttctt aaaattagcc aattaaatag taatagcgat attagtgcgg
    11521 gagataaggt gactactggt ggattaggaa actttaacgt tgctgatatt cctgttggtg
    11581 aagtggttgc cacaacgcat agtacagact atttgacacg agaagtaact gttaaattga
    11641 gtgcagatac tcataatgta gatgtgatag aattagtggg gaattcataa tgagacagtt
    11701 gaagcgagtt ggagtatttt tattgcttcc tttctttgtt ctaattgacg cccatattag
    11761 ccagcttctg ggctcatttt tcccccatgt acatttggct agtcattttc tttttctatt
    11821 tctcttattt gagacgatag aagtatcaga gtatctctac ctagtctatt gttttgttat
    11881 aggcttggtt tatgatgttt actttttcca tctaataggg attacaactc tcttatttat
    11941 cttattggga gccttccttc ataaattgaa tagtgttatt ttgttgaatc gttggacaag
    12001 aatgctagct atgattgtgc tgacattcct gtttgaaatg ggtagttatc ttttggcttt
    12061 tatggtaggg ttgacagtag atagcatgtc gatttttata gtctatag
//
LOCUS       DAVMHG010000052        11672 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMHG010000052 DAVMHG010000000
VERSION     DAVMHG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.052"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(27..1373)
                     /gene="asnS"
                     /locus_tag="VYR08_001151"
     CDS             complement(27..1373)
                     /gene="asnS"
                     /locus_tag="VYR08_001151"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV3466686.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIICATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(1530..1754)
                     /locus_tag="VYR08_001152"
     CDS             complement(1530..1754)
                     /locus_tag="VYR08_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3466687.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            complement(1747..2934)
                     /locus_tag="VYR08_001153"
     CDS             complement(1747..2934)
                     /locus_tag="VYR08_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3466688.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEKRLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(2931..3419)
                     /locus_tag="VYR08_001154"
     CDS             complement(2931..3419)
                     /locus_tag="VYR08_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV3466689.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            3740..4288
                     /locus_tag="VYR08_001155"
     CDS             3740..4288
                     /locus_tag="VYR08_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV3466690.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(4525..11589)
                     /locus_tag="VYR08_001156"
     CDS             complement(4525..11589)
                     /locus_tag="VYR08_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV3466691.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGDRGERGLTGAQGAKGEKGDRGERGLTGAQGAKGEKGAQGERGLTG
                     AQGAKGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGQAGRDGVTPTVTVKDNKND
                     GTHTITINDGRGNVTSTVVRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDAS
                     DKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITI
                     NDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEV
                     VIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDG
                     KTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDG
                     KEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEK
                     GAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQGERGLTGAQGAKGAKGEQGLQG
                     RDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARP
                     GQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVED
                     GQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKV
                     NPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERG
                     LTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNW
                     DKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDDHSTDITFTVPGKDPVTVNVKDGENG
                     LNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGAD
                     GRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEA
                     GHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTIT
                     TPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRATDAAATPTRNGIRVTVYDD
                     VNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTT
                     VVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDG
                     THTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNG
                     RDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKIS
                     VLNGDGTTTETIITDGKSPVATVRDNQDGTYTIRVENGNGTVSETTVRDGKSPTAKVV
                     DNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTGADGKGTTTTIF
                     DGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHV
                     VTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTKTVIKDGKDG
                     RDGRDGRDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPT
                     PEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPV
                     NANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
BASE COUNT         2809 a         2689 c         2163 g         4011 t
ORIGIN      
        1 aacgccacat gggcgttttc ctgttcttat ggtttgatac ggtgcaacat acgtgggaat
       61 ggaatagctt cacggatatg ttttgttcct gctgcaaagg ttaccatacg ttcgataccg
      121 ataccaaatc ctccgtgtgg aactgtaccg tatttacgaa ggtcaaggta gaattcatat
      181 tctgtacgat ccataccaag ttcatccatc ttagcgacaa gggcatcgta atcttcctca
      241 cgcatagacc caccgataat ttctccatag ccttctggag caagcaagtc tgcacaaagc
      301 acgcgctctg gatttccagg aactggtttc atgtagaagg ccttgatggc tgctggatag
      361 ttcatgacaa atgttggcac accaaagtgg tttgaaatcc aagtttcgtg tggtgaccca
      421 aagtcatcac catgctcaag atgctcgtag tcagcatctt catcattttc atgctcttgc
      481 aagaggtcaa tggcttgatc gtaagtgata cgtttgaatg gctctgcaat gtagcgtttc
      541 aagagttctg tatcacgttc caaggtttcc aaggcttgag gcgcgcggtc aagaacacct
      601 tgtagaagag ctttcacata agcttcttgc aagtcaagcg actcatcatg tgtcaagtat
      661 gagtactcag catccatcat ccagaactca gtcaagtgac ggcgtgtttt tgatttttca
      721 gcacggaaaa ctggaccaaa gtcaaagaca cgaccaagag ccatagcccc tgcttctagg
      781 taaagttgac ctgattggct caagtaggct ggcgttccga agtagtcagt ttcaaagagt
      841 tctgtagaat cttctgccgc atttcctgaa agaattgggc tgtcaaactt cataaaaccg
      901 ttcttgtcaa agaactcata agttgcacag ataatagcgt tacggatttg caacacagct
      961 acttgcttac gagagcgtag ccacaagtga cggttatcca tcaaaaagtc tgttccgtgt
     1021 tcttttggtg tgattgggta gtcttgagat tcaccgatca cttcgatgtc tgtgatgtcc
     1081 aactcatagc caaatttaga acgttcgtcc tctttgacaa tacctgtcac ataaacagac
     1141 gtttcttggc tcaagcgttt gataacatca aacttctcaa gtcccacttc ttcaccaaat
     1201 ttttcgacaa agtttggttt aaaagccaca ccttgaaaga aggctgttcc atcacgcaat
     1261 tgtaagaaag cgattttccc ttttcctgat ttattggcaa cccaagcgcc aatcgtcact
     1321 tcctgaccaa catagtcttt tacgtcaata atcgttacac gttttgtcat tatttttcct
     1381 tttctttttt attctttatg gcaaaccacc tctatattgt tcccatccag gtcaatcata
     1441 aaagcagcat agtaaatcgg acgctcactt cgataaccag gagccccatt gtctcgccca
     1501 cctgcctcta agccagcctc ataacaagcc taaacttctt ccttattttc tgctaaaaaa
     1561 gcaaaatgaa caggatcttg tgttccctga gtcagccaaa aatcaccacc aggatgaggg
     1621 ctgttcgggg atagaaaact aattagagaa ctagtcttaa aagccaattt atagtccaaa
     1681 ggagcgagaa aactcctata aaatccttct gaaatttgta aatcctttac cttaatctca
     1741 aaatgatcaa tcattctcac tacccataaa tgctttcaag cgttcgactg cttctttaag
     1801 cgtgtctagg tctgtcgcat agctgaggcg gacattttct ggtgctccaa atccagctcc
     1861 tgttaccaag gccacttcgg cttcttctaa gatagcagtt gtaaagtctg tcacatccgt
     1921 gtagcctttc atctccatgg cctttttgac atttgggaag agatagaagg ccccttgtgg
     1981 tttgaccact tcaaatcctg gcacctctgc aaggagggga tagatggtat taagacgttt
     2041 ctcaaaggcc tgacgcatgc tttctacagt atcttgctca cctgatagag cctcaactgc
     2101 tgcatattgg gctactgctg acggattcga agttgtttga cctgcaatct tggacatggc
     2161 agcgataatg tctgcttctc caacggcata accaatccgc caaccagtca tggcataggt
     2221 tttagacaca ccattgatga ccactgtttg cttgcgaatc gcttccgata ggctagaaat
     2281 cggtgtgaac tcatgaccat tataaaccaa gcggccatag atatcatctg ctaggatgag
     2341 aatatcattt tctacagccc agtttccaat tgccaagagt tcctcacggg tgtaaatcat
     2401 acctgtggga ttagatggcg aattcagcac caaaaccttg gtcttgtcag tgcgagctgc
     2461 ttctaactgc tctacggtca ccttaaagtg attgtcttcc ttagcagaaa caaagacggg
     2521 aacgccttct gccatcttga cctgatctcc atagctaacc cagtatgggg ttgggatgat
     2581 gacttcatca cctggattga ccacagccat aaagaaggta tagagagaat atttggctcc
     2641 cgcagcgact gtcacttgat ttgacgctac agaatagccg taaaagcgct caaagtagct
     2701 attgaccacc gccttaagct ctggcagacc tgaggttact gtataaaaag aagcacgccc
     2761 atctcgaatc gatgcaatgg cggcatcttg gatatttttg ggagtagtga aatctggctc
     2821 acccaaggtt agagacaaaa tatctctgcc ctcagccttc agtgctttgg cacgggctcc
     2881 agcagccaaa gtcacacttt cttccatttc taaaacacgg ttggataatt tcataggccc
     2941 tccttgttga ccaatgctcc tgtttcaaaa tctactagat aaaaatcaga tcctgacttg
     3001 acttcccaga ttggcttatc ttgataacga ccaaagataa tcttgtcaat ttcgccagct
     3061 cccttttcct tagaaaccgt ttctgctttt tcttgtgaaa caccctgatt tagctgataa
     3121 acgtaaatct tatgatcatc ttttccaatc agaacagcaa gtgcttcttg ctgtttatta
     3181 cgaccaagaa cgctgtaata agattccaag ccattgtata aatcaacctg atcggcctgc
     3241 tctaatcctg catactgctg agctaatttt tctccttcac ttttagctgt ttgatagggt
     3301 ttcatgctaa gagaaaccat atacagaaag gaagcactga taaccacaaa caaaatcgtc
     3361 atccctagac catactgcca cagtagatta ttttttgctt tgttttgtct ttttttcact
     3421 cgtctatttt accatctatt aagctttatt acaagtgaat ataagaatac tcttcgaaaa
     3481 tctcttcaaa ccacgtcagc tttatctgca acctcaaagc tgtgctttga gcaaccaatt
     3541 ctatttctcc cttcaaacaa aaccgatttt gaaagtgaaa cagttcttac tttttcagtc
     3601 acaaatgact agagtttccg aaattttaca attttcaaat aaactctgag caaatttctt
     3661 gcaagttctt ttgttttgtt gtaatatatt ttataacaac gagagagttc tcgaaatttt
     3721 aagaaaaagg agacacatca tgtctaaaaa agtattattt atcgtcggat cactacgtca
     3781 aggttctttc aaccaccaaa tggcgctcga agctgagaaa gcacttgctg gtaaagcgga
     3841 agttagctac cttgattatt cagcccttcc tctcttcagc caagatttgg aagttccaac
     3901 acatccagct gtagctgctg ctcgtgaagc agttctcgtt gcggatgcta tctggatttt
     3961 ctctccagtc tacaacttct ctatccctgg tacagtgaaa aacttgcttg actggctatc
     4021 tcgtgccctt gacttgtctg atacacgtgg cgtttctgcc cttcaagaca agtttgtcac
     4081 agtatcatct gtagccaatg cagggcacga tcaacttttc gctatctaca aagacctctt
     4141 gccatttatc cgtacacaag gcgttggtga tttcactgct gcacgtgtta atgactctgc
     4201 ctgggcagac ggaaaattgg ttcttgaaga aacagtccta aactcacttg aaaaacaagc
     4261 tcaagacttg gtcgaagcga ttcagtaaaa aatagaaaat tttagaaagt ctaaatcaat
     4321 tatagccacc taatagggtg gctttttatt tctcaatcaa gattttcaga caaacatcag
     4381 ccatccaaaa aaaccttgag cgttaccact caaggttttt agcagtcttt tccatcatag
     4441 gaaaatcatt ctaagaatat tctttcattc gagatctatc acttatcatc ttctttcgag
     4501 aaattagtga ctgcttttgg ctgattaatc ttcttttctt cgtcctacat ataggctcaa
     4561 tagaataccg ctacctaaaa gaacagagat ataatcagat tggcttccag tttcaggtaa
     4621 tttgtcccca cggttttctt tacctgtagt tggtgctact gggttagcat ttactggagt
     4681 tgctggttga gccggtgttg gtactactgg ttgttctgga accgctggta ctggtttaac
     4741 cggtacttct ggtgttggaa cagctggagt tggttgtact ggcacttctg gtgtcggtac
     4801 atttggtgtt ggttgcgctg gcacaggttt aaccggtact tctggtgtcg gcacatttgg
     4861 tgttggttgc gctggcacag gtttaaccgg tacttctggt gtcggcacat ttggtgttgg
     4921 aggaactggt ttgtcatttg atggtgttac tggtttgtct tggcatccac attttccatc
     4981 tttaccgtct ttgccgtctc gaccatcacg tccatctcta ccatctttgc catctttaat
     5041 aactgttttt gtcacagttc catcagaatt gatgattgtg attgtatgac taccatcacc
     5101 attatcttca acagaaactt ttggtgattt accatcttta ccgtctttaa tcaccatttc
     5161 tgtcttactt ccatctggat tagtgattgt aacaacgtga gttccatcgt tattattttt
     5221 aacagttaca gttggtgaaa cgctatcttt gccgtcttta ccatctttga taatagattt
     5281 gtattcacga ccatcagaat ctacgattgt taatgtatga gttccatctc cgttatcaac
     5341 gatgttcgct tttggtgatt taccatcaaa gattgtagtt gtcgttcctt taccatcagc
     5401 acctgtcact ttaatagtgt gtgaaccatc gttgttatca ataacttcaa gttttggttc
     5461 tctaccatca cgaactgtag ttgttgtagt tgttccgtct gagttcacaa ctgtgatagt
     5521 gtgagttcca tctccattat ccacaacctt agcagttggt gatttaccgt cacgaactgt
     5581 ggtttcagaa acagtaccat taccgttttc cacacgaata gtgtaagtac catcttggtt
     5641 atctctaact gttgctactg gtgatttacc atctgtaatg attgtttcag tagttgttcc
     5701 gtcaccattt agaacagaga tcttatgagt tccattttgt tcatcagtta tagtcacttt
     5761 tggtgattta ccatcacgaa ctgtggtttc agttgtcaca ccttctggat ttgtaataac
     5821 gattgtatga cttccgtctc cattatcacg aacagatgcg gttggagtac gtccgtcaac
     5881 accgtcacgt ccattcttaa caacaaactc attcttagtt ccgtctgggt tagtgattgt
     5941 tactgtgtgg cttccatctg gattttcagt agttgttgct gttgcagctt taccgtcttt
     6001 accatctttg atgatagcgt tggtaacatt gccgtctcca tctgtaactt taacagtatg
     6061 agttccatcg ttgttatccg ttacttcaac tttaggagtc ttaccgtctt taccgttttt
     6121 aacaacagtt tctttagttg aaccatctgg atttgtcacc gtaattgtgt ggcttccatc
     6181 tgggttttct gttgtagtga tattcgcagt tttaccgtct ttaccatctt taacaactgt
     6241 tgttgattca gaaccatctg ggttttgaac tgtgatagtg tgagttccat ctccattatc
     6301 ttttgtagta atagttggag ctgaaccatc acgtccatct ataccgttat aaatatcttt
     6361 actatttaat actttatcga caccttcgtc gtatacccca ttgtcattaa catcatcata
     6421 aactgtaaca cggataccgt tacgcgttgg agttgcagca gcatctgtag cgcgtctaat
     6481 tcttgaaggt gaagtaatat cttctacttt gattttcggt gcacggccat ctttaccatt
     6541 cttaccatct tttacagtga aggttactgg atctttacct ggtgttgtga tagttacaac
     6601 agtgctttgg ccatcttgtc cacgttgagt agtgattgta ggagtttgtc catcctgacc
     6661 ggttgcacca gtttctccct tagcaccatc ttgaacaaag actgtagttg gttgtgaacc
     6721 atcatggtta tcaacagtga tttctacacc tttatgccct gcttcatctt tacgaacagc
     6781 tgtaactgta ggtgattttc catctactcc atctctaatt accgcaagtg gtttttctgg
     6841 atttaatggt tgtcctgggt tagctggatc ttcttggtaa actttagttt ctttaccatc
     6901 cttgacagta agcaatgatt taccatctcg tccgtccgcg cctttagcac catctttaat
     6961 catttctgaa cctagaagtt catcagttcc tggtgtatat ttaccgtcat tgttttcatc
     7021 tgtatagaat gtcacaattg tatgagatgg atttccgttt tttccttgga cacgaagtaa
     7081 atcaactttt ggagttttac cgttcagacc attttctccg tccttaacat ttactgtaac
     7141 tggatcttta cctggaacag tgaatgtaat gtcagtacta tggtcatctt tacctggagt
     7201 tacagttact tctggagtct taccatcttc tccgcgttca cctttgtcac ccttagggcc
     7261 tttgatgttg ccctctttat cccagttgcc gttattctta acgaagacgt cgcctgtttc
     7321 tgtattaacg tatttatcgc cgtctttacc ttgatgtgct tctgggttag ctttgccgtt
     7381 caacacgtct ttaccgtcac gtccatcacg acctactgcg ccatccgcac ctttagctcc
     7441 ttgcgctcca gttaggccgc gttcgccttg gtctcctttt tcgcctttag caccatctgc
     7501 acctttgtcc cctttagggc ctttgatgtt gccttcttta tcccagtttc cattgttttt
     7561 aacatagaca tcaccggttt cggtattaat atatttatct ccatttttac cttggtttgg
     7621 ttgtggattt acttttccgt ttaatacatc tttaccatca cgacctgctt gtcctgtagc
     7681 tccatcacgg atcgtagttg tcacatctgg acttcctgga ttatgaaccg ttaatgtatg
     7741 tgtgccgttt tgcccctcag ttattgttac tgttggggtt ttcccatcag cacccttttg
     7801 tccatcctct acaaatgttg tttgtggacg acctcctact ggtgtaattg ttactaatac
     7861 accatttttg ccattttgca cacttcgtgt cgctgtaatt gttggtgatt gtccattagt
     7921 accgtttctt ccatcagtga tagttgctaa tggttggttt ggattaagtg gttgtcctgg
     7981 tctagccgga tcctctacat agacttttgt ttcagttcca tttttaacag caattagaga
     8041 ttttccatct ttacctggag ttcctggatt accttgttct ccttttggtc cttgtggtcc
     8101 cgctggtcct ctttgtccat ctgcgccttt tggtccttga gctccatcac gaccttgaag
     8161 accttgttca cctttggcac ctttggcacc ttgcgctcca gttaaaccgc gttcaccttg
     8221 gtctcctttt tcacctttag atccagttag accgcgttca ccctgatctc ccttttcacc
     8281 cttgacacct tgcgctccag tcaggccgcg ttctccttgg gctccttttt cacccttggc
     8341 accatctgca cctttgtcac ctttcggtcc tttgatgttt cctgctggtt cccaagtgtt
     8401 acctttctta acgaatacat ctcctgtttg tgtatttacg aatgtgtctc catctttacc
     8461 ttctgttgtt gggtctactt ttccgtttaa tacttctttt ccatcacgtc cagatgctcc
     8521 tgcagctccg tcttgtccgt ctttaactac tgttgtttga actaactcgt cttttcctgc
     8581 tgtgtaactt ccatctccat ctttgtcaat gtagaatgtt aatgttgttt gtttcttcgc
     8641 atcatctcgt tctgctttga cttttggtgt ttttccatct tcaccgtttt taacgattgt
     8701 tgttaatgat ggtctgttgt ctggttgtgt gattgtgatg gtgtgtgttc cgttattgtt
     8761 atctgtaact gttacttctg gtgtgaatcc atcttttccg ttatcacctt tgtcaccttt
     8821 tggtccttgt tttccatctg caattacaac ttctttaagt tttgtatctc cttcatcaaa
     8881 ttgattatta tcatttagat cataatagaa aataacagtt gttgttttat ctgcttcatt
     8941 tctatgagta gaaacaagag ggcttgcacc atcaaatcca tctcttacta ctgtagtagt
     9001 tataccacct ttaccatcgt taattgtaat tgtatgcgtt ccatcttgac catcagttac
     9061 tttaactgtt ggggttttcc catcttctcc ttttggtcct tgttcaccat cacgcccttg
     9121 aagaccttgt tcacctttgt cccctttttc acctttagca ccatctgcaa ttacaacttc
     9181 ttttaatttc ttatcagaag catctaattc attgtttcca tttttatcgt aatagaagat
     9241 aacagttgtt gtcttatctg cgtcatttcg ttgagtcgca actaatggac ttgcgccatc
     9301 gaaaccatct cttacaacag tacttgtaac attacctcta ccgtcgttaa tagtgatagt
     9361 atgagtgccg tcatttttat tatctttaac ggttacggtt ggagttacac cgtcacgacc
     9421 tgcctgacct tgggctccag tgagaccgcg ttcaccttgg tccccttttt cacctttagc
     9481 accttgtgct ccggttagac cgcgttcacc ttgggctcct ttttcacctt tagctccttg
     9541 agctccagtt agaccgcgtt caccttgggc tcctttttca cctttagctc cttgagctcc
     9601 ggttagaccg cgttcacctc ggtctccttt ttcaccttta gctccttgag ctccagttag
     9661 accgcgttca cctcggtctc ctttttcacc tttagctcct tgagctccag ttagaccgcg
     9721 ttcgcctcgg tctccttttt cacctttagc tccagttaga ccgcgttcac cttggtctcc
     9781 tttttcacct ttagctccat ctttaatagc aattttttga acaagttcgt ctacaccttc
     9841 agtataaaca ccgtttccat cgttatcaag atagaaagtg aggttatttg tatcaccgac
     9901 tttattttga tcaacacgtg gttttactgt atcgattaga tctttagcca caccaatttg
     9961 cataatacgt ggcttagctt ctttagtaac actattacta atcggcaatc ctaaaagtgt
    10021 ttcgttacca aatcgatgaa ctttataggt aatttgacgt tgtccttttt caccttctcg
    10081 aactaatcta gtttgacctt ctggtaactt actagttgga agatagacag tttggaagtc
    10141 aatgtcttct gaaacagtct tattattagt accaccataa gggtttgcat ccaagtatag
    10201 tgggtttgct ctataataat agttagagtt actgtatggg tcattgctag tagcggcacc
    10261 accaccacct tggtccacgt taaaagtaac aggtttcaaa cgaacagaca aatcaccatt
    10321 tagattaatg ataggcaaac taaatctgat agtttgttcc gtatttgttg aaatagcaga
    10381 ggtcatactg tatcctgagt gagatacgta gttggcaccc tgactaatag attttgattg
    10441 ctcccttact cttggatttg aacttgtgat cgttgcattt gaaggatttc caaatccatc
    10501 ccctttatcc aagataaaat tattattaac aaactctcta gaatctcctg gcgcagaata
    10561 actaattgta aagttaacac tattaccact acgtactgtt gttacatcgt aatgaataga
    10621 atttccacca cgtgagttcc cgtagccacg gaaactatag ctacgaccac cttggctaat
    10681 atctgcaaat gcaactgtat ttccagaacc tgagacaatt gggttggctg gattgctagc
    10741 cgctctaaaa ccactatacc ctctattaac atcttcctta cggatagctg gattttggcg
    10801 agcatcagaa aggctcaaac ctagtttcaa gatttcatta atagatgctg gagctgaagt
    10861 tgtttctact ttgtcagctg tgctagttgg caaaatagcg ctatcaacag ctgaacgttc
    10921 agttgttgca gctggtttag ccccttcttt tgattcaact gcatcagctt tcttcgctac
    10981 aggcgtttct gatacagttt ctttattaga gttagaacga agttctagac caataataaa
    11041 tgattgaata gatgactctg ctgctgataa ttcagcttca gttgcatctg cttttccaag
    11101 caagctttga ccagcttgga tacttgtttg caagttagca gcacttgctt ctgtatataa
    11161 cttgctttct aattttttag cacgcgaaag agccgttaat aattttgact tgtcaacggc
    11221 atcttgatgc gttacttcct cttttttgtc ttttgcttct tcaacttttt ctgctggttt
    11281 agtaacttct tcagttttaa ctggagtcgt ttctactgga ttagctacag ttggagctgc
    11341 ataggttttc tctttattag acgcagaaac ctctgctggt ttagtagctg cttctcccct
    11401 accaacagaa atactattgg tagtttcttg tttgttttct tccgcttgta ctgtttggac
    11461 actagctgct gcacctaata ccaaagcggt accgagcaag acactagctg caccaaagta
    11521 atacttacga atagaataac gttgttgcat gctataccag tcaaatgatt ttttatgtga
    11581 atgtttcatt ttaaactcct agatagtatt ttatagttta caatgtacaa ctatttgagg
    11641 cattaatcaa tattttctga acaattaata ta
//
LOCUS       DAVMHG010000053        11629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMHG010000053 DAVMHG010000000
VERSION     DAVMHG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.053"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            146..904
                     /locus_tag="VYR08_001157"
     CDS             146..904
                     /locus_tag="VYR08_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3466692.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            915..1709
                     /locus_tag="VYR08_001158"
     CDS             915..1709
                     /locus_tag="VYR08_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV3466693.1"
                     /translation="MKKKFFLSALLISLFSLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1722..2399
                     /locus_tag="VYR08_001159"
     CDS             1722..2399
                     /locus_tag="VYR08_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3466694.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2409..3068
                     /locus_tag="VYR08_001160"
     CDS             2409..3068
                     /locus_tag="VYR08_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3466695.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3364..4554
                     /locus_tag="VYR08_001161"
     CDS             3364..4554
                     /locus_tag="VYR08_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV3466696.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4875..5756)
                     /locus_tag="VYR08_001162"
     CDS             complement(4875..5756)
                     /locus_tag="VYR08_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV3466697.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5897..7225)
                     /gene="vncS"
                     /locus_tag="VYR08_001163"
     CDS             complement(5897..7225)
                     /gene="vncS"
                     /locus_tag="VYR08_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV3466698.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(7222..7878)
                     /gene="vncR"
                     /locus_tag="VYR08_001164"
     CDS             complement(7222..7878)
                     /gene="vncR"
                     /locus_tag="VYR08_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV3466699.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7927..8010)
                     /gene="pep27"
                     /locus_tag="VYR08_001165"
     CDS             complement(7927..8010)
                     /gene="pep27"
                     /locus_tag="VYR08_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV3466700.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8190..9569)
                     /gene="vex3"
                     /locus_tag="VYR08_001166"
     CDS             complement(8190..9569)
                     /gene="vex3"
                     /locus_tag="VYR08_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV3466701.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9621..10268)
                     /gene="vex2"
                     /locus_tag="VYR08_001167"
     CDS             complement(9621..10268)
                     /gene="vex2"
                     /locus_tag="VYR08_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV3466702.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10281..11558)
                     /locus_tag="VYR08_001168"
     CDS             complement(10281..11558)
                     /locus_tag="VYR08_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466703.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3267 a         2605 c         2205 g         3552 t
ORIGIN      
        1 aatattgtta gaaatcgatt tgactgtccc gatcgatttt tcctgttctt atttcactat
       61 agtgaatttt ttcagatatg atttgacaag aacattcttt tattgtagaa ccgtgttata
      121 aaatataaca caatagagat ctctcatggc tttagtagaa tttaaaaacg tcgaaaaata
      181 ttacggagac taccacgcat tccgcaacat caatctccgt tttgaaaaag gacaagttgt
      241 tgtcctgctt ggaccttctg gctctgggaa gtccactctt atccgtacga tcaatggttt
      301 agagactgtt gacaaaggaa gtctcctagt caatgggcac caagttgctg gtgccagcca
      361 gaaagatttg gtacctcttc gcaaggaagt cggcatggtt tttcaacatt ttaaccttta
      421 tccacacaaa gctgtgttag aaaacgtaac gcttgcaccc attgaagttc taggaattga
      481 taaaaaagaa gctgaaaaaa ccgcccaaaa atatctggaa tttgtaaata tgtgggacaa
      541 gaaagattcc tatcccgcca tgctatctgg tggacaaaaa cagcggatcg ccatcgctcg
      601 tggtcttgct atgcatccgg aactcctcct ctttgatgaa ccaacatctg ctcttgatcc
      661 tgagactatc ggagatgttc tagcagttat gcagaaactg gcgcatgatg ggatgaacat
      721 gatcatcgtt acccacgaaa tgggctttgc tcgagaggtt gcggaccgca ttatctttat
      781 ggccgacgga gaagttttag tagatacgac agatgtcgat aacttttttg acaatccaag
      841 cgaacctcgt gcccaacaat tcctcagcaa aattatcaac cacgaaagtg acaaagtcaa
      901 ataaggaggc gcctatgaaa aaaaaattct ttttatcagc attattgatt agccttttca
      961 gccttgctgc tgccaaacca gtccaggctg atacaagtat cgcagacatt caaaaaagag
     1021 gcgaactggt tgtcggtgtc aaacaagacg ttcccaattt tggttacaaa gatcccaaga
     1081 ccggtactta ttctggtatc gaaaccgact tggccaagat ggtagctgat gaactcaagg
     1141 tcaagattcg ctatgtgccg gttacagcac aaacccgcgg cccccttcta gacaatgaac
     1201 aggtcgatat ggatatcgcg acctttacca tcacggacga acgcaaaaaa ctctacaact
     1261 ttaccagtcc ctactacaca gacgcttctg gatttttggt caataaatct gccaaaatca
     1321 aaaagattga ggacctaaac ggcaaaacca tcggagtcgc ccaaggttct atcacccaac
     1381 gcctgattac tgaactgggt aaaaagaaag gtctgaagtt taaattcgtc gaacttggtt
     1441 cctacccaga attgattact tccctgcacg ctcatcgtat cgataccttt tccgttgacc
     1501 gctctattct atctggctac actagtaaac ggacagcact actagatgat agtttcaagc
     1561 catctgacta cggtattgtt accaagaaat caaatacaga gctcaacgac tatcttgata
     1621 acttggttac taaatggagc aaggatggta gtttgcagaa actttatgac cgttacaagc
     1681 tcaaaccatc tagccatact gcagattaag gaggacaccc catgacagat ttatcatctt
     1741 ggacagccta ttttcaggat tttggacaat ttttcaatgg tttcctcttc acccttgccc
     1801 tagcggttgg ttcctttatc ctcgccatgg tcttaggaat cttctttggt gccatgtcaa
     1861 ccagtaaacg tccaatttta cgcattttag ctcgcatctt tgttgaattt taccaaaata
     1921 ctcctctctt ggtgcagttt gttattgttt tttatggcct acctcttatc agtgaccata
     1981 tcatcatgat tccaatttat tggacagccg ttctctgcgt gggactctat cacggcgctt
     2041 atatcgctga ggttattcgt tcagggattc agtctattcc tagtggtcag atggaggccg
     2101 ccttgtcgca aggttttacc tatatcagtg ccatgcgctt gattaccttg cctcaggcct
     2161 tctgcatcat tctacctcca ttgaccaacc aaattgttaa cctcatcaag aacacctcta
     2221 ctgtagctat catctctgga gtagacttga tgtttgtgac taagtcttgg tcggctctca
     2281 acggaaacta tatcccagcc tttttaggtg ctgctcttct ctactttgcc ctatgcttcc
     2341 ctgttgccca gtttggtcgc aagatggagc aagccaacaa aaaagcctat tcactttagg
     2401 aggttactat ggaatctatt ttagaagttt taaccccaga taacctagtc tttatcttta
     2461 aaggatttgg cttgaccctc tatatttctc tgattgccat catcctctct actatcatcg
     2521 gtacggtgct agctgtcatg agaaatggca aaaatcctat cttacgcatt atttccagta
     2581 tttatatcga gtttgtgcgc aacgttccca accttctctg gatttttact atctttttgg
     2641 tgttcaaaat gaagtccaca ccagcaggta ttacagcctt tactctcttt acatcagcag
     2701 ccttggctga gattattcga ggcggtctca atgccgtaga caagggacaa tacgaagcag
     2761 gaatgtcaca aggcttcacc tcagcccaaa tcctctacta catcattctc ccacaagcca
     2821 tccgcaaaat gctaccagcc atcatttctc agtttgttcc cgtgattaag gataccagtc
     2881 tcctctactc tgttatcgcc ctacaagaac tctttggagc cagccaaatt ctcatgggcc
     2941 gttatttcga accagagcag gtcttcagtc tttacatcct gattgccctc atctacttca
     3001 gctttaacct agcaatttct agcctgtctc atatgctagc caaacgttgg caacaagctg
     3061 cagaataata ctcttcgaaa atctcttcaa accacgtctg cttcaccttg ccgtatatat
     3121 gttactgact tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc agcctgcggc
     3181 tagcttccta gtttgctctt tgattttcat tgagtataaa cagccactct ccagttcaat
     3241 tggagagttt ttttgatgag agtagatatt tatagacctc aatttgacac catacatttt
     3301 ctatgataaa atgtaacaaa tttatttaca acaaatcgaa atctaaatga aatggaattt
     3361 tctatgaaat caatcaaagg actactcttt atcatagcta gttttatctt gactcttttg
     3421 acttggatga acacttctcc ccaattcatg attccaggac tagctttaac aagcctatct
     3481 ctgactttta tcctagccac tcgtctccca ctactagaaa gctggtttca cggtttggag
     3541 aaggtctaca ccgtccacaa attcacagcc tttctctcca tcatcctact aatctttcat
     3601 aactttagta tgggcggttt gtggggctct cgcttagctg ctcagtttgg caatcttgcc
     3661 atctatatct ttgccagcat catccttgtc gcctatttag gcaaatacat ccaatacgaa
     3721 gcttggcgat ggattcaccg cctggtttac ctagcctata ttttaggact ctttcacatc
     3781 tacatgataa tgggcaatcg tctccttaca tttaatcttc taagttttct tgttggtagc
     3841 tatgcccttt taggcttact agctggtttt tatatcattt ttctatatca aaagatttcc
     3901 ttcccctatc tagggaaaat tacccatctc aaacgcttaa atcacgatac tagagaaatt
     3961 caaatccatc ttagcagacc tttcaactat caatcaggac aatttgcctt tctaaagatt
     4021 ttccaagaag gctttgaaag tgctccgcat cccttttcta tctcaggagg tcatggtcaa
     4081 actctttact ttactgttaa aaattcaggc gaccatacca agaatatcta tgataatctt
     4141 caagccggca gcaaagtaac cctagacaga gcttacggac acatgatcat agaagaagga
     4201 cgagaaaatc aggtttggat tgctggaggt attgggatca cccccttcat ctcttacatc
     4261 cgtgaacatc ctattttaga taaacgggtt cacttctact atagcttccg tggagaagaa
     4321 aatgcagtct accttgattt actccgtgac tatgctcaga aaaatcctaa ttttgaactc
     4381 catctaatcg acagtacgaa agacggctat cttaattttg aacaaaaaga agtgcccgaa
     4441 catgcaaccg tctatatgtg tagtcctatt tctatgatga aggcacttgc caaacagatt
     4501 aagaaacaaa atccaaaaac agagcttatt tacgaaggat tcaagttcaa ataagtaatc
     4561 atactctcct gggaaatccc aggagagttt tttatagcca aatgaaataa gacaggaacg
     4621 atggaatttt ggcattcaaa tcaattgata agactatttt atagtggagt gaaataggat
     4681 acgaacaaat tgattaggaa aatcaaatga atttatagaa atcttttagc agttatgtta
     4741 tcctattcta gtttcaaaac gctatagaag cagcattgtg ctagtctaga ttcagtttac
     4801 tatactaaaa cgagtagctt gaaatcaaaa aacccaccct cacaggcagg ttttatctgt
     4861 attattcagc tagattatgc tttaccttct gaaccgaata cgtcgatacg ttcttcaacc
     4921 gatgcttgga tagcttttac accgtcagcc aagaatttac gtgggtcgaa gagttttttc
     4981 ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt tgcgttagcg
     5041 aatgcgattt ggcattctgt gttaacgtta actttggcaa caccaagttt gatagctgct
     5101 tggatttgct catcaggaat acctgatcca ccgtgcaata cgattgggaa tcctggaaga
     5161 gcttctgtca atttttgcaa gtggtcaagg tcaagacctt cccagtttac tgggtaagga
     5221 ccgtggatgt taccgatacc agctgccaag aagtcgatac cagtttcaac cattgcttta
     5281 gcgtcttcga ttggagccaa ttcaccttta ccgatgattc cgtcttcttc accaccgata
     5341 gtaccaactt cagcttctac tgagatacct ttagcgtgtg ctttttcaac aacttcttta
     5401 gccaatttaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat tgaagtataa
     5461 ccaacttcga tacactcaag tgcatcttcg tagtgaccgt ggtcaaggtg gatagctact
     5521 ggtacagtga tacccattga ttcaacaagg ttagcgatca agttgcgagc aactttgtaa
     5581 ccacccatgt atttagcagc acccattgaa gtttggatca aaactggagc ttttttagct
     5641 tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa tccaccaact
     5701 gcataaccgt tgtcacgggc tgcttggaca aatttttctg ctgaaacgat tgccatttta
     5761 tcaggcctcc tgtatatttt tatgggtcat cccatttaca ttgttcattt tatcactttt
     5821 tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt ttcttctaac tccatctatg
     5881 taaacccttt ctctccctag tcttgggcga cttttggaaa agctataaag aaggttaaac
     5941 gattctcctc catctcgaaa cgataagcta atttttcatg ttctaataga ctcttaacca
     6001 caaagagccc cataccagac cccttgacct tgcgactggc attgtcagaa aaagactggg
     6061 ctagtttttc ttgttcctct gagctacagc tattttcgat aaaaagttct ccttctcttt
     6121 ctccaattcg aactaagcca cctggaacag agtgcttaat ggcattgctg atgagattag
     6181 aaagaatcaa cttcataact gatgggttta gataagcctg ctgatgggtc aaactattgt
     6241 ctatctggag ctctctttcc ttggctagca aggcataatc tttgaccaga ttttgcgtca
     6301 tctggaggag gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa gagagggaaa
     6361 gtatctgcag aacatggtga ttgagttcat ccacaatccc caaggcaact cccagatact
     6421 ggtctctatc cttataacga ccgatattct ctctcatatt ttcgattagg attttcaaac
     6481 tagccagcgg tgttttcaat tcatgagaag ctcctcgtag gaattcgacc ttcatcttct
     6541 ccagctggag aatggcttca ttcttttcat gcaagtccgc aataacagtc aagagatgct
     6601 ggtagaggct attgatttgt tccttgagat tacctatctc atccttagaa tccacgcgca
     6661 atcgcacttg ggaatccagg tccatcatcc gacgggtcac ccgcttgatt tccaaaatcg
     6721 gtgcaacaat agtccgggcg tagatgtagg ccaccaagag ggaaatcaga aaggaggcca
     6781 gcaaggtata gggaagaaac tggagactga tttgctccgc ttccttttgt aaatccatgg
     6841 aagctagaaa ctggagaatc atagtaccac cgtcttgcgt tttcacctcg cgctcctcaa
     6901 taaagagaga ggttgtctgg cggtctgtgt ccagaggaag actgtccttg acttctaact
     6961 tgtcctcggt catctcacct ttgacggtcc ccttgatatc actagtctgg gaatacaagt
     7021 ctaacacttg ctcgatactc tgcctatctt tcccttctag ggactgggca atggctgttg
     7081 ccttttgacc aatggtttcc tgacgatgac tcagataagt cgaaggaaaa agaaaataaa
     7141 tagctaaatg aaggcagata accagaacac taaatatcga gaaggtatag ataaatatct
     7201 ttgcaaataa acctgttcgt ttcattttcg ctccaattta taaccaacat tgcgcacagt
     7261 gaggatacaa tccaagtcta gctttttccg caattccttg atataaacat caataacacg
     7321 gtcaaaggga acctcatctg tcgctttcca gacggcatcg ataatctgag accgagtcaa
     7381 ggcccggcct tcatttttca ctagatagtc cagaatttcc aactctttgg cattgatagg
     7441 cacttcttga cctgcgaggc ttgcactgta gctttcaaag tccaccttgg tatccttgta
     7501 agaaaagatt cgtcctgtat cgtagtagcg cttgaaaatc gcgtccaccc tcacttttaa
     7561 gagggagaga gagaaaggtt tttccagata gccatctgcc aaagaggcaa aggcactcat
     7621 cttgtattcc tcatcctgaa aagctgtcaa catcaagaca ggaacctgac tggttttacg
     7681 aatctcagct aggacttcta agccgttaag cttgggcatc tggatatcca gtaaaaccag
     7741 ggccacctca tagctagaaa attgctccag agcttcctga ccgtccgctg cctcaatagt
     7801 ttcatagcca caatccgtca aataatcact gaccccctca cggatcatct cttcatcttc
     7861 tacaattaaa attttcatac tttaactgct ctctattttt tatttttctt agaataaata
     7921 cctactctat tttctattat agtctcttgc tggccttttg tctgtaagca actgatcact
     7981 agataaaacg ttgtgaaatt cctttctcat aaattccata actttagtat attatattta
     8041 agcactaaag tacaaagaaa gcaactgaaa gcaatgattt tcaccactgc tttcagattt
     8101 attttgaatt gttaaatagc tattcctatc cactattctt gaatagaaac acaagatgca
     8161 atctttattc cagactcatt ttttcaaatt tattcaccat ccagcaagag ctcttttggt
     8221 tgttttctaa ggagattgct tgaagcaagc gccataacga gaaccactag aaccaaggca
     8281 aggacaaaaa tgatgataaa gtctgatgtc tgaatggaaa tgtctaggct cgacaaggtc
     8341 ttgctaaagc catctacttc tgcaccgcca ccaaggttag aggcttgagc cgccttacta
     8401 gcctgtttgg caacacctga agtcacattg gcaaggacag tgtttccaat tgcacgggca
     8461 gtgtaattag ctaggaagta agcagaaact agagcaggga tagcaatcaa gatagattcg
     8521 gtgatgaatt gacccaagat acttgcctgc ttgaggccga tagagaggag aattcccact
     8581 tccttgcgac gggcgttgat ccaaaggctg agcaagaggg caaggaggag aactgagaag
     8641 ctcaagctac cccagaagag gaggttggcc atcttgtaca taccagagat agattgctca
     8701 agagctgggt agttagagga gctcttgacg agtgtgtagc tcttccagtt gataccactg
     8761 atgccattca actctttcat aacatcatcc aagttcttgt ctgctgttac aaagaaggtt
     8821 gcgtccccat aaatggctgt gtcttctgtg tatccataaa gttttgcagc agtgtgaatg
     8881 tctgtaatag ctgtgttttc gtaaagttct tgtgagtagg ttactgctga cttattatga
     8941 ccatcaaaga gtcccttgat tgtcacttca actgtttcct tggctccttt ttcattatct
     9001 gcatcgtaga tattagagtc cagtttaacc ttgtccccta ctttccagcc gtgtttggct
     9061 gccaagtcct tgtgcaagag gattttatcc ttgtcgtcgt tggttaagtg ctctccttcg
     9121 actagtttat aagaaccaga gacaaactta tcttctttag aggagtcatt gacacctgta
     9181 atcatcaagc tacttccaaa acgcttggca cgatcagcag tgagattctt cttggtttct
     9241 ggcgtttcaa tcaggtcata tccagtcaaa tctccgatag cgttgatacg tttgacataa
     9301 gactcgatgg ccttgttttc ggtgattttt ttgatgtctt cacccttgat attcccagca
     9361 ccacgaggcg ttccttggtt gacgcgacga ttgatttgca tggagaagct attggtgata
     9421 tttttaaagg tctcctgaga agccttggca gtagctccct tgattgacaa gccgaccaaa
     9481 ctcaagctcg ccatgaggag aataatcagg aagatgacaa tcgatttgaa aaacttcctt
     9541 gtaacatagg caaatgcgtt gtgtaacata gattcccttt ctagattttg ttttaatcat
     9601 tctatcaaaa taagctcaaa ttatttacta gtattgcgcg tttcagtcag tttcttatcc
     9661 tttaattcaa gtgtaatatc tgacgcttgt gccacttctt tactgtgagt tacgacaatc
     9721 acacatttac ctgttttctg ggcaagtgat ttgagtagtt cgacaatatc tccagcagtt
     9781 ttaggatcca gatttcctgt tggctcatca gctagaataa ctggagcttc tgagaccaaa
     9841 ctgcgagcaa tggcaacacg ttgctgttga ccacctgata actggagaac attccgcttg
     9901 atctggcttt catccaaacc aagctcaaga agtgtattct tgcttgcctt tttgttgacc
     9961 aatcggatat tttccagcgg agaaagataa tctatcaagt tataattttg aaagaccagg
    10021 gaaatatggt gcatgcgatg gtaagaatag cccttcttac gaatatcctc tccttgaaaa
    10081 aggatagaac cttcaacagg actatctaga ccagcaagta gggacaagag tgtggatttt
    10141 cctgctcctg actccccaat aatactgtaa aattttccgg gttcaaaatt ataatcgatc
    10201 tgatatagga ctgcttcagc agtattctta taacggtagg taacatcttg taattgtaat
    10261 aaagtcatga tttctccttc ttaactaata gatgataaaa tttctttcgg tgattttcta
    10321 aataagaata ggaaacaaag ggctacagat aagcaactaa gcagaactag aaaaacatag
    10381 gattctgcaa aagataagat actagttgat aaactgcttg ctttggctag tgtatcttgt
    10441 aagcttgcct gatctccact tgctagtaga gtttggagta ggtaagttgt gattccgttt
    10501 cctgcaacaa atgctggaag caaagctcca agagatacca aaactacctc taaacagaat
    10561 tgtaggaaga tcgagctctt gccttttcca agtgcaagta aaatccccac ttcatagacc
    10621 cgttctctca accagagaga caaaaccaga attaaggctc cagctcctgc tatcaacatc
    10681 ccataaagga agatggtcag gaaggtttgg aaagttgcaa ctgagtcttt gatttgttca
    10741 aaagccttgt tttccttttc gacttggtag ccttgatttt ccaaggccaa gttttctacc
    10801 tgcttcatga gtccgtccat ttccttagga ttttctacat agaagcgtgc tgcactgact
    10861 tgagcttcac tattgcccaa aagggtttgg ctactttcat agtctgtaaa gacttgattt
    10921 tcactgaagt cagaagacaa gcctgtgaat ttctcttgtt ttttaccaga aaagatgccg
    10981 ataatctcaa actctactgt ttgtcctttt ccagattcag actgaccagc atccaagcca
    11041 atcttgtcat gaagtgaaag accgttcttc ttagccaatt cttcgtggat aaggattttc
    11101 ttggaatccc ctttttgaag gtgtcgccct tcttttagat tgaaagccga actggtaaag
    11161 gttacatcct tggatgaatc ctcaagagcc gttaagctaa ccaagttatt gtctgcagct
    11221 gataaatcat cacgctccac gctctgctcg ccagtcactg cttccttgtc ttttagtttt
    11281 gcgaccgtct caagttcagg agagacattt tccagcccct taatcttgct tacagatgct
    11341 aggtctgaca acttgaatgt ctgaccattc tctatcttct taatagaaaa agatgtattg
    11401 agtgatttat aaagattgct ttctactgtt ttgttggact tcatcagagt caaacaggct
    11461 gaaattccgg ccaataagac caataaaatc agaaataaaa taaaacttct cagtcgcttt
    11521 ctgctgacat aagcccaaga tctttggatt ggattcattt gtcacctcca tatttgtaag
    11581 actattataa aacccaaata tgaaatgttt atgaaatacg aaaaaaaaa
//
LOCUS       DAVMHG010000054        11607 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMHG010000054 DAVMHG010000000
VERSION     DAVMHG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.054"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1562
                     /locus_tag="VYR08_001169"
     CDS             <1..1562
                     /locus_tag="VYR08_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV3466704.1"
                     /translation="SKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLL
                     CIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGT
                     MLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAK
                     ELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQ
                     LIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSK
                     YVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINR
                     GEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSV
                     DTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYH
                     IGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQTG
                     QQDTGDNDADN"
     gene            1549..2787
                     /locus_tag="VYR08_001170"
     CDS             1549..2787
                     /locus_tag="VYR08_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV3466705.1"
                     /translation="MRITDNQHKIAKEDFVAEYPKLSQALLDRTLDNLSREDNIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIECIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRMNKIKLIRHAYFREYRKLQELCLVILSREKHGLG
                     PQAQRVHGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGLFESLRMPHSIVHFVK"
     gene            3021..4007
                     /locus_tag="VYR08_001171"
     CDS             3021..4007
                     /locus_tag="VYR08_001171"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV3466706.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4071..4142
                     /locus_tag="VYR08_001172"
     tRNA            4071..4142
                     /locus_tag="VYR08_001172"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4104..4106,aa:Arg,seq:ccg)
     gene            4325..5023
                     /locus_tag="VYR08_001173"
     CDS             4325..5023
                     /locus_tag="VYR08_001173"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV3466707.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5014..8553
                     /gene="smc"
                     /locus_tag="VYR08_001174"
     CDS             5014..8553
                     /gene="smc"
                     /locus_tag="VYR08_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV3466708.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKDNKAELDSTEEELAQVQELLTSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLDSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTDLLNQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVYNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8550..9344
                     /locus_tag="VYR08_001175"
     CDS             8550..9344
                     /locus_tag="VYR08_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3466709.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGNKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9344..10162
                     /locus_tag="VYR08_001176"
     CDS             9344..10162
                     /locus_tag="VYR08_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3466710.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10166..11443
                     /gene="ftsY"
                     /locus_tag="VYR08_001177"
     CDS             10166..11443
                     /gene="ftsY"
                     /locus_tag="VYR08_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV3466711.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFASFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYKKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
BASE COUNT         3700 a         2021 c         2625 g         3261 t
ORIGIN      
        1 tttcaaaatt aaaatcagat ttgtatgaaa ttatcaaaga aggtattcgt tttaactttg
       61 aaaatcctat ttttgatatg aaaagatcaa caaatgaatt tattggtcgt tctgctatgg
      121 tgacaaaatt actttgtatc tatactgagg gagatccttt ctttggtgta aatattaata
      181 gtcagaaaga attttggaac cactttgttt ctcagacaaa tcaaggtgga ccttatctgc
      241 aaaatcataa aataattgaa ctggtgtcca aaacttatcc tgagttggag ccatcgaaat
      301 taggaactat gctttttgag tattctaagc tttttatgga aaataaggaa gacaatagta
      361 caatggattc atcaaacaat tttagtcatc aattaactca atctctatta aagtctccaa
      421 accttatcct tcgtggtgct cctggcacag gaaagactta tcttgctaaa gaaattgcca
      481 aagaattaac ggatggcaac gaagaccaaa tcggatttgt acaatttcac ccatcatatg
      541 attatacgga ttttgtagaa ggtttaagac cagtatcaaa tggggatgga gctattgagt
      601 ttaggctaca ggacggtatt tttaaagatt tttgtcagaa agcaaaagaa acccaattga
      661 ttggaggaca agataatttt gatgaggctt gggattctta cttagaatat ataaatgttg
      721 ctgaagaaaa agaatatata acaaaaacat cttacttatc tgttaatagt agacaaaatt
      781 tatcagtaaa ttatgatagt ggtgttccag gatggtcact acctagcaaa tatgtttacg
      841 agttgtataa agataaaaat tataataagc aagaatacta caaaagtggt ggaaaaactg
      901 tcctagaaac attgagaaag agatttggtt tgaaagacta tgtttcccca acagaaattg
      961 atactgataa gaaattcgtt ttcatcatcg atgaaatcaa tcgtggggag atttctaaga
     1021 tttttggcga actctttttc tctatcgacc ctggctatcg tggtgaaaaa ggaagtgttt
     1081 ctacccaata tgcaaatcta cacgaaactg atgaaaagtt ctatatcccc gaaaatgttt
     1141 acatcatcgg aactatgaat gatattgatc gttcagtgga tacctttgat tttgctatgc
     1201 gtcgtcgttt tcgttttgtt gaagttactg ccgagggtca agttggcatg ttggataaag
     1261 agctgaatat ccatgcagaa gaagcaaaaa ttcgtctaag aaacttgaac gctgctatcg
     1321 aaaatgttca ggaattaaac agtcattatc atattggacc aagttatttt cttaagttga
     1381 aggatgtaga ttttgactat gaattactct ggtctgatta tattaagcct ctcctagaag
     1441 actacttgcg aggttcttat gatgaggttg aaactttgga aactttgaaa aaagcatttg
     1501 aactgacaaa taatgatcaa actggtcaac aagatactgg tgataatgat gcggataact
     1561 gataatcagc ataaaattgc taaagaagac tttgtcgcag aatatcccaa actaagtcaa
     1621 gctcttcttg atagaacact ggataacctt tctagagagg ataatatctt tatttttcca
     1681 aatgatttga ctcatactcc tgatttggat aaggaccaaa agatttttga aacagtcaat
     1741 cagaaaatca agacagggaa cgtgattggt tttcttggat atggtcagga aagattaacg
     1801 atttcctcac gattttctga tgagagtaat gaccattttt tgcattatct cttaaacaag
     1861 gttcttcata tcaatctcac tagtttagat gttgctttgt ctcgtgaaga gaggctttat
     1921 caacttttga tgtatctctt ccccaagtat ctacaagctg ctattcgaaa aggtctttat
     1981 aaggaatatc atcgattttc tcataacgac agtcatgtta agggagtaat tgatgtaaga
     2041 aaccatctca agaaaaatct tcctttcacg ggaaatattg cctacacaac gagagagttc
     2101 acctatgata atcccctcat gcagttggtc cgtcacacta ttgaatgcat taagaatcag
     2161 aaaagcattg gtcaaggggt actagataat ctctcaacta gtcgtgaaaa cgtatctgaa
     2221 atcgtgcgtg taacgccctc ttataaacta gctgatcgtg ctaagattat cagaatgaat
     2281 aaaatcaaac ttatccgaca cgcctatttc agagagtata gaaaattaca ggaactctgc
     2341 ttggtgattc tgagtagaga aaagcatggt cttggacctc aagcccaaag ggtacatggt
     2401 attctctttg atgttgcctg gctttgggaa gagtatgttt acaccttgtt gccaaaaggt
     2461 tttgtacatc ccagaaataa ggataagacg gatggaattt cagtattttc tgttgggaaa
     2521 cgaaaagtat atccagattt ttatgataga gaacgaaaga ttgttctaga tgcaaaatat
     2581 aaaaaactgg aattgactga aaaaggaatc aaccgtgagg acttattcca gctgatttcc
     2641 tattcttata ttttaaaagc tgagaaggct ggacttgttt ttccgagcaa ggacaaggtt
     2701 attgataatg agataggaaa cctagcaggt tatggtctat tcgaatccct cagaatgcct
     2761 cattctatag tacattttgt aaaatgatgg aaaattcaga agaaaatttt aaagcgatta
     2821 ttgatgaaga agtggggaga aaataatttc ccctctttct tttattttta acacaagtaa
     2881 aacccgaacg atatatttta attcataaaa taatttgaca aaaactgtac tttggtttat
     2941 aatgaattaa ggaaacgtcg gaaaaggcgt agtgatgcaa gagcataggt aggtcattac
     3001 aaaagaaacg agacatcgat atgttaaatg aatttccaat ttttgattac gaagatattc
     3061 aattgattcc aaataaatgt gtcattaaaa gccgtgcaga agcggataca agtgtcactt
     3121 taggaaatca cacctttaaa ctacctgttg tgccagcgaa tatgcagacg attttggatg
     3181 aaaatgtagc agagcaactg gctaaaggtg gttacttcta cattatgcac cgttttgatg
     3241 aggcaggacg cattcctttt attaaacgaa tgcacaatca agggctcatt gcttccatat
     3301 ctgtcggtgt taaggactat gagtatgatt tcgttagaca gctcaagact gatgctcctg
     3361 agtatatcac tattgatatt gctcatggtc atgcggatag cgtgatttct atgattcaac
     3421 acatcaagaa agaattgcca gatacctttg tcattgctgg aaatgtggga acaccagaag
     3481 ctgtgcgtga attggaaaat gctggtgcag atgctactaa ggtcggaatc ggtcctggta
     3541 aggtttgtat caccaaggtt aagactggtt ttggtacagg tggttggcag ttggctgctc
     3601 tacgctggtg tgccaaggct gcgcgtaaac cgattatcgc tgatggagga attcgtactc
     3661 acggtgatat tgccaagtct atccgcttcg gtgctagcat gatcatgatt ggttccctct
     3721 ttgcaggaca tattgaaagt ccagggaaaa cgattgaagt cgatggtgaa cagttcaaag
     3781 aatattatgg ttcagcctca caatatcaaa aaggtgctta caaaaatgtg gaaggcaaac
     3841 gtatcttgct tcctgctaaa ggtcatctgc aagacacttt aactgagatg gaacaagacc
     3901 ttcaaagtgc tatttcttat gcaggtggac gtcaggttgc tgaccttaaa cacgttgatt
     3961 atgtgatcgt gaaaaactcc atctggaatg gggatgcttc ccactaagac ggggtctttg
     4021 accaaaaaac ttgttattag agcaaatttc tgttataata aaacaagttt ccacccttag
     4081 tgtaatggat atcacgtaag attccggttc ttgagatggg ggttcgattc cctcagggtg
     4141 gatgtaaaca tcctaaaaag cctttaatag ggcttttttc tttatcctga cccaaatttg
     4201 ccgctaaaac tgagaatctc tgtctaaaga attcccaaat gctttccagt cccgttttag
     4261 agtgactcag ggggcttttt ttgatataat aaaaaggact gttatcagtt agaaagaggt
     4321 tggtatgaaa gaattacaaa ctgtactaaa gaaccatttt gcaatcgaat ttgcagacaa
     4381 aaatttactg gaaactgcct ttactcatac gagttatgcc aatgagcacc gcctcttaaa
     4441 aatttcacac aatgaacgct tggaattttt aggagacgct gttctacagt tattgatttc
     4501 agaatatcta tataaaaaat atcctaaaaa gcctgaaggt gacctatcaa aactccgtgc
     4561 tatgattgtc cgtgaggaga gtttggctgg ctttgcgcgt gattgccagt ttgaccagtt
     4621 tatcaagctg ggtaaggggg aagaaaaatc tggtgggcgc aatcgtgata ccattcttgg
     4681 tgatgccttt gaagcctttc ttggtgccct tcttttggac aaggatgtgg ccaaggtcaa
     4741 ggaatttatc tatcaagtca tgattcctaa ggttgaagca ggcgaatttg agatgattac
     4801 agactataaa acccatctcc aagagttgct tcaggtcaat ggtgatgtgg ctattcgtta
     4861 tcaggtgatt tctgaaacgg gtcctgctca cgataaggtc tttgatgtag aagttcttgt
     4921 tgaaggtaag agcatcggtc aaggtcaagg tcgttctaag aaattagcag agcaggaagc
     4981 tgccaaaaat gccgttgaga aagggctgga ttcatgtatt taaaggaaat cgaaattcag
     5041 ggattcaagt cttttgctga taagaccaag gtcgtctttg accaaggtgt gacggcagtc
     5101 gttggaccca atggatctgg aaagtcgaat attacagaaa gtctgcgatg ggccttggga
     5161 gagtctagtg tcaagagtct ccgtgggggc aagatgccgg atgtcatctt tgctggaaca
     5221 gaaagtcgta aaccgcttaa ttatgcttct gtagttgtga ctctggataa tcatgatgga
     5281 tttatcaagg atgcaggtca agaaatcaga gtagaacgcc atatctatcg tagtggggat
     5341 agtgaataca aaattgacgg caagaaagtt cgtcttcgtg atattcatga tctcttcttg
     5401 gacacaggtt tgggacgaga ttccttttcc atcatttccc aaggcaaggt tgaggagatt
     5461 tttaattcta agcctgagga acgccgagct atttttgaag aagctgcagg agttttaaaa
     5521 tacaagactc gtagaaaaga aactgagagc aaattgcaac aaactcagga taatctggac
     5581 cgcttagagg acattatcta cgagttggat aatcaaatca agcctcttga gaagcaagct
     5641 gagaatgccc gtaagttttt agacttggaa ggacaacgta aggctattta tttagacgtg
     5701 ctggttgctc aaatcaagga taataaggct gaactagact cgacagaaga agagttggct
     5761 caggttcagg aactcttgac tagttattac caaaagcgtg aaaaattaga agaggaaaat
     5821 caaactctta aaaagcaacg ccaagatttg caggctgaaa tggccaaaga ccaaggcagt
     5881 ttgatggatt tgaccagtct gattagtgac ttagagagaa aattagccct atcgaaactg
     5941 gaatctgagc aagtagccct caatcaacag gaagcacaag cccgtttggc tactttggag
     6001 gataagagaa gttcactcag ccaagaaaaa tctgataaag aaagctcttt agctctgtta
     6061 gagggaaatc tagtccaaaa taatcaaaaa ctcaatcgtt tagaagctga attgctggct
     6121 ttctcagacg atcctgatca gatgattgag ctcttacgtg aacgctttgt agctctttta
     6181 caagaagaag cggatgtctc aaaccagttg acccgtattg agaatgagtt ggaaaatagt
     6241 cgtcagcttt ctcaaaaaca agcagatcaa ctagaaaagc tgaaagagca attggctata
     6301 gctaaagaga aggctagtca gcaaaaggaa gagcttgaaa ctgccaagga gcaggttcag
     6361 aaattattgg ctgactacca agctattgcc aaggagcaag aggagcagaa aacttcctat
     6421 caagctcaac aaagccaact ctttgaccgt ctggataatc tcaaaaacaa gcaggccaga
     6481 gctcaaagtt tggaaaatat cctgagaaat catagtaact tttatgcagg tgttaagagt
     6541 gttctccaag aaaaagatcg cctaggtggg attattggtg cagtcagtga gcatctgacc
     6601 tttgatatgc attatcaaac tgccctagaa attgcacttg gagcgagtag tcagcacatt
     6661 attgtagaag atgagaatgc ggcaaccaaa gccattgatt tcctcaaacg aaacagagcc
     6721 ggtcgtgcaa cctttcttcc tttgaccact attaaggcgc gtacgatttc tagtcagaac
     6781 caagatgcta tcgctgtaag cccaggtttc cttgggatgg cagatgagtt agttactttc
     6841 gatactagac tggaagccat tttcaagaac ttgctagcta cgacggctat ctttgatacc
     6901 gtagaacatg cgcgtgaagc tgctcgacaa gttcgttatc aggttcgcat ggtgacattg
     6961 gatgggacag agcttcgtac aggtggttcc tatgcaggtg gagccaatcg tcagaacaac
     7021 agcatcttca tcaagccaga actggagcaa ttacaaaaag aaattgctga agaagaagca
     7081 agcttggatt cagaagaagt ggctttgaag accttgcaag atgagatggc cagattgacc
     7141 gagtcattag aagctattaa atctcaagga gagcaggcac gtattcagga gcaaggcttg
     7201 ttcctcgctt atcagcaaac tagtcagcaa gttgaagagc tagaaactct ttggaaactc
     7261 caagaagagg aaatagatcg tctttccgag ggagattggc aagcggataa ggaaaactgc
     7321 caagagcgtc ttgctgcaat cgccagtgac aagcaaaatc tggaagctga gattgaagag
     7381 attaagtcta acaaaaatgc catccaagaa cgctatcaaa acttgcagga agagctagcg
     7441 caagctcgtt tgcttaagac agaactgcaa gggcaaaaac gttatgaagt tgctgatatt
     7501 gagcgtttag gcaaggaatt ggataatctt aatatcgaac aagaagaaat ccagcgcatg
     7561 ctccaagaaa aagttgacaa tcttgagaaa gttgatacgg atttgctcaa tcagcaggcc
     7621 gaagaagcca aaactcagaa aacaaatctc caacaaggtt tgattcgcaa gcagtttgag
     7681 ttggatgata ttgaagggca gctggatgat attgctagtc atttggatca ggctcgccag
     7741 cagaatgagg agtggattcg caagcaaaca cgtgctgaag ctaagaaaga aaaggtcagc
     7801 gaacgcttgc gccatctaca aagtcaatta acagaccagt accagattag ctatactgaa
     7861 gctctagaaa aagcgcatga attggaaaac ctcaatctgg cagagcaaga agttaaggat
     7921 ttagagaagg ctattcgctc actgggtcct gtcaatatag aagctattga ccagtacgaa
     7981 gaagtttaca accgtctgga ctttctaaat agtcagcgag atgatatttt gtcagcgaaa
     8041 aatctgctcc ttgaaaccat tacagagatg aatgatgagg ttaaggaacg ctttaaatca
     8101 acctttgaag ctattcgtga gtcctttaaa gtgaccttta agcagatgtt tggcggaggt
     8161 caggcagact tgatattgac tgagggcgac cttttaacag ctggtgtgga gatttctgtt
     8221 caacctccag gtaagaaaat ccagtcgctt aacctcatga gtggtggtga aaaagctcta
     8281 tcggctcttg ccttgctctt ctccattatt cgtgtcaaga ctattccatt tgttatcttg
     8341 gatgaggtgg aagctgcgct ggatgaagct aatgttaaac gttttgggga ttacctcaac
     8401 cgctttgaca aggacagcca gttcatcgtc gtaacccacc gtaagggaac catggcagcg
     8461 gctgattcta tctatggagt gaccatgcaa gaatcaggtg tctcaaaaat tgtttcagtt
     8521 aagttaaaag atttagaaag tattgaagga tgacaattaa actagtagca acggatatgg
     8581 acggaacctt cctagatggg aatggacgct ttgatatgga tcgtctcaag tctctcttgg
     8641 cttcctacaa ggaaaaaggg atttactttg cggtggcttc gggtcgggga tttctgtctc
     8701 tggaaaaatt atttgctgat gttcgtgatg acattatttt catcgcggaa aatggcagtt
     8761 tggtagagta tcaaggtcag gacttgtatg aagcgactat gtctcgtgac ttttatctgg
     8821 caacttttga aaagctgaaa acgtcacctt atgtagatat caataaactg ctcttgacgg
     8881 gtaataaggg ttcatatgtt ctagatacgg ttgatgagac ctatttgaaa gtgagtcagc
     8941 attataatga aaatatccaa aaagtagcga gtttggaaga tatcacagat gacattttca
     9001 aatttacaac caacttcaca gaagaaacgc tggaagatgg ggaggcttgg gtaaacgaaa
     9061 acgttcctgg tgttaaggct atgacaactg gctttgaatc tattgatatt gttctggact
     9121 atgtcgataa gggagtggcc attgttgaat tagttaaaaa acttggtatc acaatggatc
     9181 aggtcatggc ttttggagac aatcttaatg acttacatat gatgcaggtt gtgggacatc
     9241 ctgtagctcc tgaaaatgca cgacctgaaa ttttagaatt agcaaagact gtgattggtc
     9301 accataagga ccagtcggtt atagcttata tggagggctt ataatggcag atataaaatt
     9361 aatcgcattg gacttggacg ggaccttgct gactactgat aaaaggctga cggatcgtac
     9421 caaggaaacc ttgaaagctg cgcgtgatcg tggtatcaag gtcgtattga caactggtcg
     9481 tcccttaaaa gctatggatt tctttctcca tgagctaggg actgacggtc aggaagatga
     9541 gtataccatt acttttaatg gtggtttggt tcagaaaaat acaggtgaaa tccttgataa
     9601 gacagtcttt tcatatgatg atgtggcacg cttgtatgaa gaaacagaga aattatcact
     9661 gcctcttgac gctatctcag aaggaactgt ttatcaaatt caatcggacc aagaaagtct
     9721 ttatgccaaa ttcaatccag ctttgacttt tgttccagtt gattttgaag acctgtctaa
     9781 tcaaatgacc tacaataaat gcgtgaccgc ctttgctcaa gaacccttgg atgcagccat
     9841 tcagaagatt tctccagaat tgtttgacca atatgaaatc tttaaatcac gtgaaatgtt
     9901 gctagaatgg tcaccaaaga atgttcataa agcaacaggt ttggcaaaac taattagcca
     9961 tcttggaatt gatcaaagtc aagtgatggc ctgtggtgac gaggccaatg acctctctat
    10021 gattgaatgg gcagggctcg gtgttgccat gcaaaacgct gttcctgaag tcaaggcagt
    10081 cgcaaatgtg gtaactccta tgaccaatga tgaagaagct gtcgcctggg ctatcgaaga
    10141 atatgtgcta aaggagaact aagatatggg attgtttgac cgtctattcg gaaaaaaaga
    10201 agaacctaaa atcgaagaag ttgtaaaaga agctctggaa aatcttgatt tgtctgaaga
    10261 tgttgatcct accttcacag aagttgagga agtttctcag gaagaagcag aggttgaaat
    10321 tgttgaacaa gctgtgttcc aagaagagga aatccaagac acagttgaag aaagtctgga
    10381 tttagagcca gttgtagaag tttctcaaaa agaagtcgaa gaatttccac actcagaaga
    10441 agggaatact gagtttctag agactgtaga agaaaataat tctgaaattc ttgaagcaga
    10501 aaggcctcaa acagaagaaa ctgttcagga aaaatatgac cgcagtctta agaaaactcg
    10561 cacaggtttc ggtgcccgct tgaatgcctt ctttgctagc ttccgctctg ttgacgaaga
    10621 atttttcgag gaactggaag aactgttgat tatgagtgat gttggtgtcc aagtcgcttc
    10681 taacttaacg gaggaactac gttacgaagc caagcttgaa aatgccaaga aacctgatgc
    10741 acttcgtcgt gtcatcattg agaaattggt tgagctttat aaaaaggatg gtagctacga
    10801 tgaaagcatc cacttccaag ataacttgac agttatgctc tttgttggtg tgaatggtgt
    10861 tgggaaaaca acttctatcg gaaaactagc ccaccgctac aaacaagctg gtaagaaggt
    10921 catgctggtt gcagcagata ccttccgtgc gggtgcagta gctcagctag ctgaatgggg
    10981 ccgacgagta gatgttccag tagtaactgg acctgaaaaa gctgatccag ccagcgtggt
    11041 ctttgatggt atggaacgtg ccgtggctga aggtatcgat attctcatga ttgatactgc
    11101 tggtcgtctg caaaataagg ataaccttat ggctgagttg gaaaagattg gtcgtattat
    11161 caaacgtgtt gtgccagaag caccacatga aaccttcttg gcacttgatg catcaacagg
    11221 tcaaaatgcc ctagtacagg ccaaagaatt ttcgaaaatc acacctttaa ccggaattgt
    11281 tttgactaag attgatggaa ctgctcgagg aggtgtggtt ctagccattc gtgaagaact
    11341 caatattcct gtaaaattga ttggttttgg tgaaaaaatc gatgatattg gagagtttaa
    11401 ctcagaaaac ttcatgaaag gtctcttgga aggtttaatc taatcagaag caaaaatcct
    11461 gggctctttg tcaactgtag tgggttgaag tcagctaagc tcgagaaagg acaaatttcg
    11521 tcctttcttt tttgatgttc agagcgatga aaatccgttt tttgaagttt tcaaagttcc
    11581 gaaaaccaaa ggcattgcgc ttgataa
//
LOCUS       DAVMHG010000055        11409 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMHG010000055 DAVMHG010000000
VERSION     DAVMHG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.055"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..698)
                     /locus_tag="VYR08_001178"
     CDS             complement(<1..698)
                     /locus_tag="VYR08_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466712.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(708..1460)
                     /locus_tag="VYR08_001179"
     CDS             complement(708..1460)
                     /locus_tag="VYR08_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466713.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(1460..2224)
                     /locus_tag="VYR08_001180"
     CDS             complement(1460..2224)
                     /locus_tag="VYR08_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466714.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(2320..2724)
                     /locus_tag="VYR08_001181"
     CDS             complement(2320..2724)
                     /locus_tag="VYR08_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466715.1"
                     /translation="MKRRKIYSLFCVVTIASSLFLPTVLARQIGDYDLTLPRWGSDTT
                     SELEKENASAGINNNDSTGGGKTLNTSIRSAYSGSDITPVYSLGSGSRIVMYYNGGGD
                     NYIGSGTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            complement(2904..3536)
                     /locus_tag="VYR08_001182"
     CDS             complement(2904..3536)
                     /locus_tag="VYR08_001182"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV3466716.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSDLIHLAQEEGYITPEGLYLLKRFKEDQENWQEG"
     gene            complement(3570..4271)
                     /gene="pyrF"
                     /locus_tag="VYR08_001183"
     CDS             complement(3570..4271)
                     /gene="pyrF"
                     /locus_tag="VYR08_001183"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV3466717.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPV
                     AAYHAIKDEWTQDWN"
     gene            complement(4504..5703)
                     /gene="divIB"
                     /locus_tag="VYR08_001184"
     CDS             complement(4504..5703)
                     /gene="divIB"
                     /locus_tag="VYR08_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV3466718.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(5713..6771)
                     /locus_tag="VYR08_001185"
     CDS             complement(5713..6771)
                     /locus_tag="VYR08_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV3466719.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(6775..8127)
                     /locus_tag="VYR08_001186"
     CDS             complement(6775..8127)
                     /locus_tag="VYR08_001186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV3466720.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(8231..8872)
                     /locus_tag="VYR08_001187"
     CDS             complement(8231..8872)
                     /locus_tag="VYR08_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466721.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIAPHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(8874..10895)
                     /locus_tag="VYR08_001188"
                     /pseudo
     CDS             complement(8874..10895)
                     /locus_tag="VYR08_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(10978..11277)
                     /locus_tag="VYR08_001189"
                     /pseudo
     CDS             complement(10978..11277)
                     /locus_tag="VYR08_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         3611 a         2458 c         1944 g         3396 t
ORIGIN      
        1 cattctataa acctcctctt ttgacgagtg aaatagaaaa tgcttttaaa aagaaagact
       61 aaaaatagca actgaagaaa taaatctcgt cctatatctc cattccctcg attcaaaata
      121 taaaatagat aattagttcg atttcctaca aatagaccac caaacacaat catgagtaaa
      181 aagaaactaa cgcaagcaaa gttcatttgt acattcgctc ctagtgcgta taacataaag
      241 caaaacaaga ctaacacaca agcattcaaa gaatcaacta taaacaaaac tcctatatcc
      301 cagtttggta actgtaacat aaattgaatc actagaactt ttaccatgat taaaatgagt
      361 ccataaagaa aacaggtcca aatctgatac ctaatccaga gcttaataga agaaaatcga
      421 atcacttcca taaagaaatc ggatgctctt ctacgatata caactgctag aaaaaatgta
      481 tgaataataa accaatgaag caaccatata gataagacta aaaaagagtt ttgactcaga
      541 ttaggccctt ttaaaatttc tataacaagt ctatcatttc cttctaacca ataccataat
      601 cctaaacaaa tcaatatcca aaaaagaaca taaccccaaa agtacttggt atagattatc
      661 tgattcaacc tccacatgtg actacttaac tttcccattt ccaactttta cactctcctc
      721 cagtttctat gaacagaaaa tacagtcaat ggaattatga tagatagtaa tgtaagccaa
      781 taaatcaatg catacggatt ggaatttacc atatactgaa aaattggcaa taatccaatt
      841 atcccattgt ccaactgata aatccagaat aaggattcta caaataaaaa agtaaacaga
      901 aatactaagc tcatgtttga ttttttaaac caagaaaaac tgctaatagt aagtaaggat
      961 aaaaagaaat agtatgctat ataagagaaa aaaaatccta taaagaaact agcattgttt
     1021 gcaatactta taccataaaa ttctcttaaa aaatcaacct cctttatctc caaagagaag
     1081 ctaaaaccat tactaaatgc aattagaaaa atcaataaaa ataaagtcgc cgtccaaatc
     1141 cccgtactaa gagctgctaa tttgaaacta aaactggtaa agtgcttaat tgatttcaga
     1201 cgaatgcgac actccaacct attaaaatag ttattcatca aataaaaaaa gaacaatata
     1261 tatgtgaacg gaaagcaata tactccagtc gtcatatctt gaagtaaaac taagatccat
     1321 tctaatacat ttggatggat tgaatattgg cgacagcgca ataaatatac tgtactagat
     1381 aaaacacagg atagcagtaa tataaaataa accaatactg ctaagaaatc ttttttataa
     1441 aattgaacaa attgtttcat tatacatagt cctctgaatg tagaaaaaat gtaccataaa
     1501 caaccaaaca actaacaaat aaaataaaag caagatgagc actaactaag gaaagactga
     1561 tatctttctg atatcccaaa gctagcgttg tcacaggttc taagtaagat agccctaaaa
     1621 tagcccataa aataccacca accatcatat aggcaactgg gatgaaaata gctcctaatt
     1681 ttttcttcac tagcaaagca ctagctagtc caaaaataga gaacacagcg ccccaaactc
     1741 cataccagag agtcgtcaca agactataga gcaactgatt ggaatcaaat aattctttta
     1801 aggcaccact ataatctcca atataaattt cctgataagg agtcactaaa agattaattc
     1861 ctaataataa taaatagggg agaaaaaaga ctagaaaaga agaaataatt gcagtactac
     1921 ctacaatagc tagatacctc tttttagaaa ttcggcacaa ttgtgctgtt agaaaatgac
     1981 tctcagcatc ctctattatc tgactagaat agggcagtgt acagataagt gcagctacta
     2041 ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat aaaccttcaa
     2101 ttttataaat accattgaaa gcaaggaaat ttcctaaact catgcaaaga agggtcaaaa
     2161 ataaagacat ataaaatcga ggtgattgaa cgactcgtac aagattacaa atgaaaaatt
     2221 ccatccttac tcctccttat aataaaaata gggtgtagca ttcttttttc atgctacacc
     2281 cacaatcaac catctttaag gcttactctg acaagtaagt taataagaat ctggactcca
     2341 agaacctgaa gtatgaattc ttacatgatt tccaaattgt ggcgccatag ctaatctagt
     2401 accagaacca atataattgt caccacctcc attatagtac atgacaatcc tagagccaga
     2461 ccccaatgaa tataccggag taatatctga cccactatag gcgctacgaa tagaggtatt
     2521 taatgtttta ccgccaccag tactgtcatt attattaatt ccagcagagg cgttttcttt
     2581 ctcaagctca ctagttgtat ccgaacccca acgtggcaac gttaaatcat aatctccaat
     2641 ctgtctagct aaaactgtag ggagaaacaa actagatgca attgtaacta cgcaaaaaag
     2701 ggaatatatt tttcttcttt tcatgacatc gtcctcctaa gaagtgataa ataagatagt
     2761 tgattgatca atttgataca tattatattt tcattatatc aacaaaagaa agcgttgtca
     2821 attattttgt ttaaaatatt gtcatatttt aaacaaaatg gtaaaagagg ctcggtagcc
     2881 tcgtctctct ttatcttact gacctaacct tcttgccaat tttcttggtc ttctttaaag
     2941 cgttttagaa gataaaggcc ctctggcgtg atgtaacctt cttcttgggc tagatggata
     3001 agatcgctat agtttgaaag cgtcacaagt ttaacaccag catctgcaaa gttcttatct
     3061 gcttttggca attggtagct gaaaatcgct acaactccaa gcacatctgc tccttctcgc
     3121 ttggctgctg ctacagcttc aagaactgaa ccacccgttg aaataaggtc ttcaaccact
     3181 accatttttt gaccttgagc tacgcgacct tcgatttgat taccagctcc gtggtctttt
     3241 ggtttgctac ggatgtaggc aaaaggcaag tccatcttat cagcaataat ggctccgtgt
     3301 ggaatccctg ctgttgcagt tcctgcaatc acttctactt caggaaaggc ttctttgata
     3361 gcttccacaa aaccattttc aattagggta cgagtttctg gataggctag tgtcacacga
     3421 ttatcagtgt aaatcggtga cttgatacca gatgcccaag tgaagggttc ctctggtttg
     3481 aggtaaacgg cttggatttt caagaggtgg ctagcgatat ctttagcaag tgtcatggta
     3541 ttctcctttt atttttctaa tctagttctt taattccagt cctgtgtcca ttcatccttg
     3601 atggcatgat aagctgcaac aggctcctca gcttgggtaa tgggacgtcc cactacgata
     3661 tagtcactgc cgatttgata ggcatcagca ggtgtcatga ctcgtttttg atctccaact
     3721 gcaacaccag ctggacgaat ccctggtgtc agacagataa aatctggatt ggtagcctgc
     3781 ttgatgactt gtacttcctg agccgagcaa acaacaccat ccaagccagc ttcagctgtc
     3841 ttcttggcat agtgaatcac agactcttgc agactggttt ggatattttg aaactcctgc
     3901 atctgagctt ctgacgttga tgtgagctga gttacagcga tcaatttagc ttgacttcca
     3961 agaccttcac gcgcagcctt catcatctct acaccaccag ccgcatgaac attggtcata
     4021 tccacaccaa gctgagatag gatcttcatg gctgacttga ctgtattagg aatgtcatga
     4081 agtttgagat ccaaaaagac actatgaccc aaacctttta agtaggacac aatctcaggc
     4141 cccgctgcgt aataaagctc catccctacc ttgagataaa ggctttcttc tgctgggaaa
     4201 agagctaaaa attccttgac cgcctcaaaa ctaggaaaat caagagcaat gattggacga
     4261 tgttctcgca taggtttctc cttttacctt tactagtgag agagtctggc caacagaagc
     4321 cacgaaaaaa ggaacctgcc aacagcaagg ttccacgaaa aatgggtagt ctccaccctt
     4381 tcaccgtcta accttagctg cctctctgga ctgctttaaa ggttttttac tattctatta
     4441 tttctactag cacttgtcaa gtaaaaacat ggtcactaaa gaactataag agaaaaggta
     4501 aacctagcga cgcgatgagc gctgggtcgt ttggtttcga ttgctctctt cctcttgttt
     4561 tttctgttct tcttcttgtt ttttctcagc ttccttagcc tcttgtttgg ctttttcctc
     4621 agcttccata attaatttat ccgccacagt gtaactgtaa attccagctt ccatgtcaat
     4681 cacactcggt tctgacaatt gtggcttaat cttactataa taaggcagtt tcttgctcat
     4741 ttcagaaagc ggaaccaaga cttcgtccaa atcattcata gtcaatcgaa ttaaatcgga
     4801 tgtcaccttg cttggggcta attccacctt ttggatagcc gccttgagtt ctgggctaat
     4861 ttgagcaagt tctgagacaa aaaccttgat ttgttcacta tcattaaaga gaactgataa
     4921 ataagtttct ggtaaactgt tcaggctcac agaactagtc tcaagctgac cactggaaag
     4981 aataggataa tgattttcac cagaaatata gtaggccaca atatcatatt ccttgacctt
     5041 aatagtgaac ttagttggaa attgatagac aagttgagct gattcaaccc aatagttaga
     5101 cttaatctgc ttttcatatt ttgccttgtc tagcagaagg ttaatcgtat aatccgaatc
     5161 ctgaatgcct gaagcctgtc gaatatcatc agctgtagtt tgcaccgttc cctcaacacg
     5221 aatatctttc atggtcgcat aaggactgag caagtaggca gagacaatca ataaaagcag
     5281 acttggaaat aaaatcgtga aggctcgcaa gatatggata ccaggaatct ttgctttggc
     5341 tggtttttcc tttgtagcct ttttagcaag ctttttatcc tgttcctcct tctctttaga
     5401 ctctggttct tctttctctt ctttctcttt gtcatcctct gaggatgcta ctttttcttc
     5461 agactcttcc ttagctgatt ctgaatcttc ctggtctgtt tcactctcct ggtcctgttt
     5521 gtcctctgac ttctcagatt cttctcccat ccgagcttgt ctttcctttt ccttctcctc
     5581 agctagagcc gcttcttctt cagccttctt ttttagatat tcttggtttc gtttctgcca
     5641 ttctgataac tctttcaatt cttcgaggat ttctttgtcc tcatttttct tatcttttga
     5701 catttacttt ccttatgata aatctttttt caacaattga taaaaatctg ctagagattt
     5761 caattcctta gaagctttca tcttagcttg gtaatcttcc ttgtgactta gtaagtgaga
     5821 aagcttctct tccaaactat ccaaggtcaa atcgctttct tgaaggtctt ctgcatagcc
     5881 tttttttaca aagtaagctg cattttcaat ctggtcacca cgactagctt cacgaccaag
     5941 cggcacaatg acatgcaatt ttgctatcgc caagagctca aaaatcgtat tggcaccacc
     6001 tcgtgtcaca acaatatcag ccaattccaa caagggttga tagagatcgg tcacatagtc
     6061 aacacgaaaa agattttggc tcaactcatt caggctagaa tctccagtta gattgataat
     6121 attgtagcgc tctgttagtt ctttcttatg gtctgtcacc aattggttaa agacacgagc
     6181 acctgcagaa ccgccaacaa acaatacagt cggcaattta tgattaaagt gggtttgaat
     6241 atccaccaat tcatctggtt ctggattttt ttgatctgaa accttggtca ctgctcccac
     6301 atgctcaacc ttagacaaac tcgaagcttg ctcaaaggtt gaatacatct tagtcgcaaa
     6361 tttataggcg attttattgg ccaagcccat agacaggtca gattcgtgaa taaagacagg
     6421 cactcctgac acacgcgctg cgataacagg cggtactgag acaaagcccc cctttgaaaa
     6481 aagggtctgt ggacgaagtc gcaacatgat aaagagcgat tggacgattc cccagccaac
     6541 tttgaagacg tccagcatat tttgccaaga gaaatagcga cgcaatttcc cagtcgcaat
     6601 ggagtggaaa gtgacatcca aacctgactt aaggatttct tggtgttcga taccacgctt
     6661 gtccccgata tagtggactt cccaaccatc ttcgatgaac ttgggcatta acaaaagatt
     6721 gagggtaacg tgtccaaccg tccccccacc tgtaaagaca atttttttca tattttattc
     6781 ttttaactcc gctactgtgt cgataaagag gtcgccacgt acttcaaagt tagcatacat
     6841 atcccagctg gcattggcag gactaagaag aaccacatct ccttgagtcg caagctcata
     6901 ggccttgcgg gtcgcatctg caatatctgt cgcctccaca taagcgacac cagccttgtc
     6961 tgctgcccgt ttgacacgtt ctgcagattg acccaggatg accatcttct tgagtccagt
     7021 aatgtctggc accaattcgt caaactcatt gccacggtcc aaaccacctg caatcaagac
     7081 gaccttgctg ttgtcaaatc ctgataaggc tttttgagta gccaagatat tagttgattt
     7141 actgtcgtta tagaatttaa cacccttgat gtcatccaca aactggagac ggtgtttgac
     7201 accaccgaag gctgaaagag tttccttgat ggtttgattg tccacatcac gaagcttggc
     7261 tacagcaata gtcgcaaggg cattttccac attgtggcta cctggaacac cgatttcatt
     7321 cgctgccatg actacttcac cacggaagta gagttgacca tcttccagat aagctccatc
     7381 aaccttttca agtgttgaaa atggtacaac agtggcttct gtcttggaag ccaagtcttt
     7441 taccaagtct tgattaaagt tcaagacaag gaaatcagct gctgtcatct tgttctggat
     7501 attccacttg gctgctacat attcctcaaa taatccatga taatcgatat gagttggcat
     7561 gaggttggta ataaccgcaa tctctggatg gaattcttga acacccatga gttggaaaga
     7621 agaaagttcc ataacaagcg tgtccttatc tgatgctatt tgagcaacct gactggcagg
     7681 atagccgata ttccctgata aaagaccatg ttgcccagca gcagtcaaaa cttccccaat
     7741 catagtcgtt gtggttgtct taccgttcga tcctgtgata ccaataatcg gtgcttctga
     7801 aatcaaataa gccaattcca cctcagtcaa gactggaatt cccttggcca aagccttttc
     7861 aatcatggga ttgctgtagg ggatacctgg attttttacc atgagggcaa actcttcatc
     7921 caagagttcc aaaggatggc cacctgtaat gaccttgatc ccttcttcca gcaaacattg
     7981 ggcagctgga ttgtcctcga atggtttccc atcatttact gtcacaatgg cacctaactt
     8041 gtccaacaaa cgagccgcag attcaccaga cttggccaaa cctaaaacaa gaactttctt
     8101 atttttaaat tgatctatta ctttcatgtc tcgaactcca tttctactcc tactatttta
     8161 ccatttttat ggaaataaaa aagccacaaa gtgtgtttgt gactctttct tctaactgaa
     8221 tcttaccata tcatctatgt gataaatcgg taactcgaat gacctgatcc acttgctccc
     8281 aaatcagagg attgtggggc gcaataataa tggtccgatt cggatttttt aaagattcta
     8341 ggatggaaag taattcctca gaatttttgg ggtctaagga agcggttggt tcatctgcaa
     8401 gaatcaaagg cggatccttt aaaattatct tcgctagtgc aacacgttgt gcttctcctc
     8461 ctgataactc aaatataggt tgcttcaaat ccaaataaga gaggtttaca cagtttagag
     8521 cttgtttcat caaagagatt ttctcttttt ccttcaactt tttaccaact aaacccagat
     8581 tgagattctc tttgacggtt tggctttcaa ttaagccaaa atcttgaaat aagtatccta
     8641 agtaatctct aaagaaaaca gaaggcttga tgtccttaag agaagtgcca tcatagatga
     8701 tttgcccttt gtcatatggc tccaatcgtc caatcatatt caagagtgtt gtcttaccac
     8761 agccacttgt accgattaag gcataaattt tcccaccttc aaaatgaaga ttcgtatctg
     8821 aaaatagctg acggcttcca aattttttag atatattctt tagttcaatc atcctatttt
     8881 cctttcataa ttgtcataga aacacgagat tctttctgcg cttgacggta aagcgtcaaa
     8941 actgcactag ctagaaagac caataaagtg agcaagccaa tcaccaagtc tcgactgctt
     9001 aaaataaaga gactagcacc aaatacaaaa ctggcaaatt ggctaaccat atactgagca
     9061 tgtgtttcaa aaaatcgtaa acctgaaatt cgtttaatca agatatctcg gcggaattgc
     9121 tcgaaatata gaagattgac agaataaaat agtaacaagg aactagctat ccccacaata
     9181 gctcctaaga ttaaagttgc tgtttcagtt tgaacttcat tataacgagt tagataaaca
     9241 cttcttcctt ctttaagata ggatacttgc tcataaattc cagctttctt caagagttct
     9301 agcccactct catatccttt gataaagagt tgttttccag cattgataga ccaactagat
     9361 aaggatataa aactatcacc tgtagaagtc ggcgtgaata ccactaaaat cggatcagtc
     9421 aaatactgag tagatacggg attctcaccg ttattataaa caaaccgctt ttctcccatt
     9481 gaaagataac taacgtgcgc tttcatctca taatccaaag gagcacttgc ctcctcacca
     9541 gattttccat aataactcaa tctttcttca aaaactttct taagttctgc ttctcgagag
     9601 cgcaaatgtt ctgggagcaa gaggataaac tcaccttttt ggagatgggc taacttctgt
     9661 ttggtctcag catctaccac gaccttttcc ttgtccaaat aactgggact aacatagagc
     9721 gtattagcat ctggactata ggtatccagt gtctctccct gttcattttt tccttgtgga
     9781 ttggcaaaat ggagcagatt atcctttaca taaagagctt gttcctcttc gattgcttcc
     9841 ttggcaaagg cataccactt gctctgattt tctgtatctt ttcctctatc acctaagcca
     9901 aaagaaattt gataatagtc tgctctatcc tgccatgctt gttttgaaat ttcaagttct
     9961 ttcaatcgtt ggtaagacgt caaacctgtc ttaacagcgt atcctactgt aaagacagca
    10021 accaactgac acaatagggt taaaatcatc aggcgtttaa ggggtaatct ccccttaata
    10081 acgggaacta atgctttgta actcaaactc attagataaa gaagcattag taaaattgaa
    10141 atcgccaata aaaacaacag atagaaacta atcccaaaac cataggtggc taacaagata
    10201 ggataaaaca aaccttgact aaaaagaacg actcccccac ctaggaagga aaggagggct
    10261 gatagaagga gctatttgat atcagtagat aaagaatgcc ccatgatgga taagagagtc
    10321 tgaccagaaa agagttttat acctgctgct ctcatttcct taatccgagt gataatcact
    10381 aaagcaaaga aagataagcc aaatattgct aaactaatta aaataagggg atttagtaat
    10441 attcgaaaag caagaaaata gggcggtatc tttcggtcag cacttgcttt ataacccaaa
    10501 tctcctaatt tatcggcaag cttttctttc gtcaaggagc ctgacaaaag gagataacta
    10561 tttagcggat cactacgttc acgactttct tggctagctt cttggaattc ttttggtaaa
    10621 gttccctgac cataagttgc ataagtaaag tgagtcgtcc catccttact cggctctaca
    10681 attcttctag ctattaaact ctgttctgag tttgcaaaat tctccaattc ctgttcaaat
    10741 acctcacgcg tcggttcctg agtatctttt ttgacacgaa gtaaagaaac ggaatcatag
    10801 cttgcatata aatattgtgg cgcacgtaag acaataatcc aagcaaggaa gaagctgaga
    10861 aaaaaagttg ataataatat gaatagtttc ttcatagtag actccttggt agactccttg
    10921 taaacaaaat tccccctgta atttcttaca aggggaacga tttaaatcaa tgaacgatta
    10981 gtcataatca cagtaaaatg ctacttgttc tccccattta gtccaaatcc atgcatagga
    11041 ggtttttcca gcaggagctt ctcctttatc agaatgacca tcccatctgc tcacgatgga
    11101 tgaataatga tattttttac catgatagta atttgaaaaa gcctaaccac ctcctgaacc
    11161 ttctccatat gtccatactc ctccatctgg atattataca gcagctgatg cagctcccaa
    11221 taatgtaaaa cttgaaataa gagctagagc aagtaatcta tgttttttcg ttttcatttt
    11281 attttttctt tcaaaaaaat cacaccttga gcaacaatgc aacaaaataa atcctcctct
    11341 ctcttttatt gaaaccgctt tcttatgtga taagaataac atttttatta tttgttgtca
    11401 aggaaaaaa
//
LOCUS       DAVMHG010000056        11168 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMHG010000056 DAVMHG010000000
VERSION     DAVMHG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.056"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(540..1733)
                     /locus_tag="VYR08_001190"
     CDS             complement(540..1733)
                     /locus_tag="VYR08_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV3466722.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1871..3313)
                     /gene="gtfA"
                     /locus_tag="VYR08_001191"
     CDS             complement(1871..3313)
                     /gene="gtfA"
                     /locus_tag="VYR08_001191"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV3466723.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(3590..3670)
                     /locus_tag="VYR08_001192"
                     /pseudo
     CDS             complement(3590..3670)
                     /locus_tag="VYR08_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3671..4504)
                     /locus_tag="VYR08_001193"
     CDS             complement(3671..4504)
                     /locus_tag="VYR08_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3466724.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4518..5384)
                     /locus_tag="VYR08_001194"
     CDS             complement(4518..5384)
                     /locus_tag="VYR08_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3466725.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5398..6657)
                     /locus_tag="VYR08_001195"
     CDS             complement(5398..6657)
                     /locus_tag="VYR08_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3466726.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTGRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6692..8854)
                     /locus_tag="VYR08_001196"
     CDS             complement(6692..8854)
                     /locus_tag="VYR08_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV3466727.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIYKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLEHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDIVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            8962..9822
                     /locus_tag="VYR08_001197"
     CDS             8962..9822
                     /locus_tag="VYR08_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3466728.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9819..10754
                     /locus_tag="VYR08_001198"
     CDS             9819..10754
                     /locus_tag="VYR08_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV3466729.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(10880..10967)
                     /locus_tag="VYR08_001199"
     tRNA            complement(10880..10967)
                     /locus_tag="VYR08_001199"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10931..10933),aa:Ser,seq:gct)
     gene            complement(10974..11047)
                     /locus_tag="VYR08_001200"
     tRNA            complement(10974..11047)
                     /locus_tag="VYR08_001200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11011..11013),aa:Ile,seq:gat)
     gene            complement(11083..11153)
                     /locus_tag="VYR08_001201"
     tRNA            complement(11083..11153)
                     /locus_tag="VYR08_001201"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11119..11121),aa:Gly,seq:tcc)
BASE COUNT         3622 a         2381 c         1932 g         3233 t
ORIGIN      
        1 aagtgtcaaa gactacagtg aaaataggaa atttgacaca gaaattttgg agcttaggaa
       61 gacatacagt aaaatgaaat acggacggaa caatgtgatt ttggaattca aattaaatta
      121 taacaatatt gtagaagtat cattctagta ttcaagattc agtttactat gtcttttcac
      181 accaacctta ttccgaattc aattactttc gtgatttaca catatagatt aagactatct
      241 tttatacttt aaaatttctc gctaccttgt ccactatatg ctcctcgcta tcacgtttct
      301 attcatagcc tacgatttca ctattgcttt ctctgacaat tcttatttcc tgcgtcagac
      361 ttaaaacgat ctatccccag accattttaa tccgctacct cacgatagtc aggcttgggg
      421 agcgctattg tattcaccgg tagtggatcc ctacagagga cttacacctc agatgcacga
      481 catgcccatc gtataaaaaa atctcctacc caaggtagaa gatttcaaac ttataaaact
      541 taatccgtca tgtccgatac caacattcga tgctccaatg gaatactgca cataactagc
      601 aagaaaataa agcctgactg aatccagaag agagccaagt caaaaattcc gtgcacagca
      661 accactgtaa ggaaagatag ataaaggccg ataatcggac gtttccccga ctcctgactc
      721 atatccatca tcaaacgaac aggagcaaca gaagacagaa ctaataaaat ggtacccaca
      781 attccgtaac tcagaatcgt atcaatataa agactgtggg catgttcatg ataaggagca
      841 tgtatccgag gataagagtg catataggtc aatggccctt caccccaaaa aggattttgc
      901 ttaaacaagg ccatcccagc atcccagata gaaatgcgtt cttccataga agagtctaaa
      961 gtacccattc gaactcccaa atcactagaa aagaggaaac tcaaaccaat cgcgaagacc
     1021 ccaatactaa gccaaaaagc cttccagttt ttaatagtcg taaagagata gataattgct
     1081 ccagcgataa tagcaggaaa ggcagttcga ttttgagtaa agttcaaacc aaagagatta
     1141 acaaagcctg caatcacaca gaatactttc aaccaattca acttggtcgt tgtaaacaga
     1201 tagaaagcaa tcataataca gaaacaacaa ataattccat aataattagg attaaagaag
     1261 gtcacttctg cccggttctg atgccacacc tgcatattgg gtgaaagaaa agcatagtta
     1321 aatttcttca caatttggaa atgttctaaa ctggcaaaag cagctgacaa gacactacca
     1381 aacaagacga actgcaaaat caatcgaaag aatttatggg ataaaatcga ctgatagtgc
     1441 aaaaagaaaa tagtaaatag aaacattcct actgaagcca caagacccat ccaattttgt
     1501 gcaagaatgg atataacagt actatagcta agaaaaagaa gcagcatcgg atgctccccc
     1561 attttctgaa gaatactttt catgtctcct gtaaaaatca aactgataat atataaacag
     1621 agtacaacta caaaaagata aaagggtaaa aagatactca ggataattcc caataaaatc
     1681 agctctttac tagacaaccc cttcagcttt tcaataaagc ctattgattt caaaatgaat
     1741 cctttctctc caaatcagct gattcagata atagtaagct atcctatatt gtaccacttt
     1801 tttagcaatt tgaaaacaaa ggaaacgttt tccaaaataa aaaccctatt ttatccacca
     1861 tatcaaggct tcaaaatgtt acttcaactc cattctcaat tacccgataa gtctgatttt
     1921 gcaaatcaat ttctgctact gctgttacgg acttatcttt attttgacgt ttgattacaa
     1981 tgctgtgaac tgttggtgtc tctatctcaa tagtcccttc tagatcaaag gcttctgaac
     2041 ggttacggaa agaaaataga ttgagaaggg ccttcacaac agatcgttgc acttcttttg
     2101 ctatttcctc gttgctatag taatgacgat taatatttcg accttcttta gtttcttcta
     2161 ataatttcaa gtcattcttg cctgctaata gacccacata gtaaacctga ggaatacctg
     2221 gggcaaaagc ttgaattaga cgagcgagaa aatacttgac atcatcatct ccaagcgctg
     2281 aatagtaggt tgaattgatt tggtagatat ctaagttgtt atactcggca ctagagtact
     2341 tacgtttgac attggctcca accttataga gttcatttga agcatagtca atctcctcat
     2401 cggtcaggat atccttgaca tctactactc caatcccatc atgggtatct agcgtcgtaa
     2461 attgcttcat cgggctcatc tttaaccact tagccaaacg ctctgttctg gaactgtaaa
     2521 gagtataaag tgtcaccatt ggaagagcaa aatcataaac atagtaatca tggtctgcta
     2581 ttttaaactg aatcgaatag tgttcatgaa tctcaggtaa aagctctgtc ccatactcag
     2641 cagcgatatc tcgaactttg tccaataaat cccaaatatc tggttccaca aagaaatcat
     2701 tagtatccaa tttcttcact gcataagcaa aggcatctag acgaatcaaa tcacacccat
     2761 tacttgccaa gtgctgaatg gtcttacgga taaattccat agttacttct ttggtcacat
     2821 caagatcaat ctgctcctca ccaaaggtat tccacaaatg ttccactgaa ccatcttcaa
     2881 acacaatctc ttgctttggt gcacgatcct tacgcttgta aattaaatct acatcagact
     2941 gtgtcggacg gttttctggc caaaacttat cccagtttaa aaagagatct ttaaattcac
     3001 tggcttcatg tttttcttga tagtccttat aatacttgga ttgacgagaa atatgattaa
     3061 tcataaaatc aaacataaga taatatctct cacctaaacg cttaacatcc tcccaatcac
     3121 caaaagctga gtccacttcg tcgtagtcaa ctggcgcaaa tccacgatca cctgttgatg
     3181 ggaaaaatgg taaaaggtga actcctccaa tagcatctcc aaaatgctct tccaaattat
     3241 catataagtc tttaagatta tttccaaggc tatcagaata ggtaatcaac atggttttat
     3301 tttgaattgg catcattact ctcctttttc taattgaagc caagtctcat atgatctgcc
     3361 ttcataaata aaattcattt taaatctcta tttatcatca aactcgtact aatatagact
     3421 gtgataaaca aagtactact ttcttgtttt ctacatagaa ttatcaacaa gctgaactct
     3481 tcctctgtgt caaagactat agattccatg agctcttctt atactattcg aaaatctctt
     3541 caaaccacgt cagcttcacc ttaccgtagg tatggttact gacttcgtca gtttcatcca
     3601 caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt
     3661 tcattgagta ttacttcact gccccgttgc tcattcctga aatgatatgg cgttggaaga
     3721 agagatagac aatggtgata ctgataatgc cgaccacgta agaggcaaag cttggtccgt
     3781 agtcgttgaa atattggcct gcgtagttgt attggaacaa aggcagagtc cacattttgg
     3841 aatcccggtt caagacaagg agtggcaaca tgaagtcatt ccagaaccaa agggcattga
     3901 tgatcatggt tgtcgcatgc atcggtttca tcattgggaa gatgatgcgg aaataggttg
     3961 taaattgatt agccccatcg atctctgctg cttcatccag actttctgga atcgagattt
     4021 tgatatagcc aacatagaga aagagggtct gtggaatcgc ataggtcaag tagagcaaga
     4081 tcaaaccaaa ggtattagcc aaaccgagtt tactcatcat aaccgtaatc ggaatcatga
     4141 tgacttggaa aggtacgaag attccgagga ttaagagggt atacatgatg gtaaaggctt
     4201 ttcttttact catattgcga gcgatggagt aggctgccat agggataaag atcattactg
     4261 caagtaaaga caagacagtg atgacgacag agttccaata atagcctcca atcccatcag
     4321 ctaagagacg gctaaagttg tcccatgtga agttggttgg aaagccaaag aaattatcta
     4381 caatatcctt agtggatttg aaggaactaa agagggtagc aaggagcggc actaaaatca
     4441 gaaccgatcc tagaatcaat agaatgtatt tgccaatcag ggcttttctt tcatcttgtt
     4501 tcatcatgct tctcctctta aatttcaaat ttcttagata ctctcaattg gatgatcgaa
     4561 atcactacaa ttaagaagaa caagattacg gcaatggcat tggcataacc gaattggttg
     4621 tttttaaagg catagttata aaccaaaagc ccaagtgagg ttgtggcatt gtttggacca
     4681 ccaccggtca tggcaaagac ttggtcaaag gcagtcagcc caccttttag ggctaggata
     4741 aagactatag agacacttgg tagcaagtaa ggcaattcaa tgttccagaa aacttgcttg
     4801 ctagtcgcac catcaatcct tgctgcctct gtaatctcag ttggaataga ttgcaaacca
     4861 gctaggaaga tgatgatggg catagccacc ccttgccaaa gaaggacaaa gacagccgca
     4921 aagattgctc cccacttagt ccctaaaaga ctggtttgga gaaattcaat atgaagggca
     4981 ttcccaatcg ctggaagacc gtagttgaag acttgcttga agatcaaagc cactgtcaaa
     5041 ccagataaaa cagctgggaa gaagaaccaa gcacggaaga aggtttggcc tttgatttta
     5101 gagttcaaga cacgcgcaat gaagatcccg agtgcaatct caccaaccac catggcaatc
     5161 gtaatgattg cggtaaagcc aatcgcattc atgaattttg gatccatgaa gaggagctta
     5221 aagttgttta agccgacaaa tttgtagttg taagtcaatc ctgtccagtt ggtaaaactg
     5281 taaaaggctc cttgaaacat cggcacatag aagaaaattg cttgtaacaa gagggggatg
     5341 accacaaaag cccatgccca atatttttgt aatacttttt tcatagtctc tctactccta
     5401 atccacatcc gctttcatcg ggttaaagaa ggcattcaaa tcattgacca tgccttgttt
     5461 atcaccggtc aagacatagt tcatggtcaa ggtatggaag tctgcttcac tggtccagta
     5521 ttgttgcaac cagaccaagt gacgacccgt aaaggcatat tcggtcatac cagcaagcgg
     5581 tgaatcttct cctgcttgtt tgaccccttc gatcgctgtt ggagatccgt ccacatcgta
     5641 gtatttttgc atgacttctg gacgggtcat atattccaca aaggcattgg cttcttttgg
     5701 atgtttggtg gtggctgaga tagaccatgc caagtctccc gcaccaacgg ttaagctttg
     5761 tcctttttct tttcctggaa tcatgaaggt cccaatctta aagttcggtt tttgttcatt
     5821 aatcgctgtg atcgcccaag acccatttgg tgtcatgagg acatccccac gtgcgaaggc
     5881 tccgataaca tcggtatagc cagcaccttc ccagttcttt tgcttagagc cattgatgcg
     5941 aaggatgtcc atgaccttga tatcatcttt cataatcgga tccgacaatt taatggcatt
     6001 tggttgagaa taacgaaggt attgatttgc ttcttttcct ccacctgttg ctgtcgcaaa
     6061 ggctaattga ttgtaaccat tgagtgtcca agcatctgca cctgcaattc caaatggtgt
     6121 ttgtccttta gcaacgatat ctttgactaa ctgttcaaat tcatcccagg tttcaggaac
     6181 cttcaagccc agttcttcga atttatcttt gttgtagtaa attccataag cattagctgt
     6241 aaaaggaacg ttgtaaactt tttcgtttac agcatatttt tcagcgtagc catttttcac
     6301 gcgtttcagg tagtctttgt tgctcaaatc ttcaaagaca cctgcttttg cccattcttg
     6361 cagttcgatg gactgtgggt aaatattgac cacatcaggc acatctcctg cgagaacgcg
     6421 tgtcttcaat acttcaccag catttggtac attgacgact ttaaccttga tcttagggtt
     6481 ttccttctca aaatcacgag tgatttcttc caaggttttg gtcatttctt ttttctggtt
     6541 gaaatactcg atggtcactg tgccatccgc agatttacca tagttggagc aagcgccgag
     6601 cccaaccaaa gctaaacctg tagttgcaag aagtccgatt tttttatacc attccattag
     6661 aaagcctcct ttataaattt atacaccctt atcatagtct tctaaaaata tactgtctac
     6721 tcaaaaaatc tccttgggat aagataacag ttaagcccgc atacattagt tctgcacctg
     6781 agtaaacttc gccattttcc tgtaatttat atagtccctc ttcatccaaa tcttttaatt
     6841 ttaaagttgt ttccatggtc tctacaacag ataaaacgcg aacgtaggtt acaatcgttt
     6901 gatttccgta attaaattgt acagctgctt cattggatac aatatcagga ttaattagtc
     6961 tatactgctg tcctaactga actactggtc gtaattcttt atacaagttc acctgattag
     7021 caatcgtagc tttctcttca tctgataaat ttgtcaaatc aagttcatag cccaaatttc
     7081 ccatcattgc tacaaggcca cgtgtttcta atggtgtcat tcgtcccatc tgatgattcg
     7141 gtactgctga cacatgagcc cccatagaaa tggttggata gagataggat gaaccgtatt
     7201 gaattggtaa acgtgcaatg gcatcagtat tatcactagc ccagacttgt gggaaatagc
     7261 gcatcatacc aagatcattt cgtccaccac caccagagca ggactcaaag agaatatggc
     7321 tgtgcttctc tgtcagataa gaaacgagtt cgtaaagccc cagcatgtac tgatgagatt
     7381 gcatctgtgt ctctagataa gttaatccat tccctagctt agtgatattg cggttcatat
     7441 cccatttaat gtaatcaata tcatgataag ataggagttg atctaagaca cttttcaagt
     7501 attctactac ctgaggattg gcaagattaa gtactaattg attccgagaa taagtatgct
     7561 catagccagg aacctgaata gcccagtcag gatgttgacg atacaaatca ctatctacag
     7621 aaatcatttc gggttctaac caaagtccaa actgcaaacc tctttcatgg atagctgaaa
     7681 tcagactttc tagacttcca cccagttttt cctcattaac aacccaatca cctaaagcac
     7741 gattatcatc aaaacgattg ccaaaccaac catcatctaa tacaaaaagt tcaatgccaa
     7801 ctttcttagc ttcatctgct aactctaaca gtttttctct ctgaaagtca aagtaagtag
     7861 cttcccagtt attgattaga attggacgtt cttttttaga aaattcactt agcataatgt
     7921 gctccagtac aaaattctga ctttcatgac taataccagt taatccctga tctgaatgag
     7981 tcactaaagc taccggtgtt tcaaagtatt cctcaggggc taacttccaa gaaaagtttt
     8041 ctggattaat gccaatagcc acccgaactt cattcaattg atttttttga acaaaagctt
     8101 caaagttgcc actatacatt agttgaatag caaacacatt cccagcatcc tctgtgactc
     8161 cttgttcgca tagtagaaga gctggtgttt gagcatgacc agaagcacct cggtttgaac
     8221 taatcgaaaa gattccttgt tctacctgtt gacgtctaac agtcttttca cgagcataag
     8281 caccctgcag agttactatt tcgtaatctg cagctggaaa atcagccata aaagaaaaat
     8341 ctttatagat gacaacttcc tgattactat tattatctaa tttactgtag ctagcaatag
     8401 tcgcatcatt attaaaagta gtataataca aagtcagact aagttgagcc ttagaatctt
     8461 ctaacattaa gacaagagtc tctgtatcgt ccatgctatg tggagaaggt aagccctgtg
     8521 gaccattctg accttttaaa atctttgctt ctacaaatcg aaagtctgtt acttcagtta
     8581 cattatgctg aacctgtatg gttggtttcc taaaatctcc taagccatgt tgtccaaaaa
     8641 tttgacgttg agtatctaaa ctaaaggttc gattagtagc cgttggattt cctgaaaagg
     8701 catggtctcg ttcataaaca ctattggaac ctttatagtt cttaatagtc tttcctaaat
     8761 gtttcaaaag taagtagcca tttcgatttt caataatcaa acttagattt ttactctcaa
     8821 cataaaatag attattctct atcctaactc ccatttactt cacctcatca ctttattgat
     8881 tatattttat cacctgaaat cgctttccaa aatagaaaaa tgtctcaaga atatggtaaa
     8941 atgttaggta ggaggtagca catgttagtt ttttcagaat accagactgg aacaatcgac
     9001 cttgccctaa gcttttatgg atatgaggaa tgcacaccta attactcttt tggtccagcc
     9061 attcgtgata catacgttct acattacatt actaaaggac aaggaaaatt tcattacaag
     9121 ggtaaaattg ttgatttaaa agaaggagat ttctttctat taaaaccaga ggaactaacc
     9181 ttttatcaag cagatagtaa agaaccttgg gcctactact ggttaggaat cactggaggg
     9241 aaagcccctg attattttgc tctttcccaa atttctgatc aatcctatct catccaatct
     9301 gaaacttgtc atacccagac tactgcaaaa ctcatctcag acattgtccg cttcgctcag
     9361 attacaaaat caagtgaatt agctcaactc catatcatgg gacaacttca tgaactgatg
     9421 tttcatctgg gaactattgc tcccaatcag aaaaaaaaga atatttcatc aacccaccaa
     9481 ctctatcttg aatgcaaacg attaattgat agccactatc ctcaatcact tacaattcaa
     9541 gatttagcaa aagaactatc cgttcacaga agctacttat caagcgtatt caaagaattt
     9601 aataccttat cacccaaaga atacctactc tacgttcgaa tgcaccgagc tagacaactt
     9661 ctcgaaaata cccaagagtc catcaaggta attgcatact cggtaggttt ttcagatcca
     9721 ctccattttt cgaaagctta taaacaatac tttaatcaga ctccaagtca tacaagaaaa
     9781 gaatactctc aataccaact agtaagaaag gcaacattat gaaatcctac caagctgtct
     9841 accaaatcct atctaaagaa accgactata tcagcggaga aaaaatcgca gaaaaactat
     9901 ccctaagccg aacagcaatt tggaaagcca tcaagcgact agaacaagaa ggcattgaaa
     9961 ttgatagtat caaaaataga ggatataaac tgatgaatgg tgaccttatt cttccagaga
    10021 ttctagaaga aaatcttcca attaaagtca gctttaaacc cgaaacaaaa tcaacacaac
    10081 tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa taccctctat ctagcttcct
    10141 atcaaacagc aggccgaggc cgttttcaac gttccttcta ctcaccacaa ggtggtattt
    10201 atatgacact ccatcttaaa ccaaatctcc cctatgacaa attaccatcc tacacactac
    10261 ttgtagctgg agctgtctac aaagccatta agaacctaac tttaatagat gtcgacataa
    10321 aatgggtcaa tgatatctat ctaaacaatc ataaaattgg aggaatcctt actgaagcaa
    10381 tgacctctgt agaaactggc ttagtcacag atatcattat tggagtaggt atcaatttca
    10441 ctattaaaga cttccctcag gaattaaaag aaaaagctgc cagcttattt aaagctacag
    10501 ctcctataac aaggaatgaa ttgatcatag aaatctggcg tgctttcttc gaaacaccag
    10561 cagaagagct attataccta tacaaaaaac agtcattcat tctaggaaaa gaagtcactt
    10621 tcacactaga gcaaaaagac tacaagggac ttgctaaaga catctcagaa aatggaaaac
    10681 ttttagttca atgtgataac ggaaaagaaa tctggctaaa tagtggcgaa atttctctca
    10741 atagttggaa gtaaaataac acaattataa tataaacgat ataaaaataa cttcagatta
    10801 gtaattcaat taagttttac ggatctgaag ttttattggc tctaaaaata aaaaagagag
    10861 ttacaaactc tcattaaaac ggagaataag ggattcgaac ccttgcgcca gttacccgac
    10921 ctaacgattt agcaaaccgt cctcttcagc ctcttgagta attctccaat taatgggcac
    10981 gagtggactc gaaccaccga cctcacgctt atcaggcgtg cgctctaacc acctgagcta
    11041 cgcgcccaag ttaaaaaact tggtaatttg aacaaagttc aaagcgggtg acgagaatcg
    11101 aactcgcgac aacagcttgg aaggctgtag ttttaccact aaactacacc cgcataaata
    11161 ctatcaat
//
LOCUS       DAVMHG010000057        11145 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMHG010000057 DAVMHG010000000
VERSION     DAVMHG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.057"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            34..1296
                     /locus_tag="VYR08_001202"
     CDS             34..1296
                     /locus_tag="VYR08_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV3466730.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(1589..2059)
                     /gene="nrdI"
                     /locus_tag="VYR08_001203"
     CDS             complement(1589..2059)
                     /gene="nrdI"
                     /locus_tag="VYR08_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV3466731.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(2137..2670)
                     /locus_tag="VYR08_001204"
     CDS             complement(2137..2670)
                     /locus_tag="VYR08_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466732.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            complement(2722..4008)
                     /locus_tag="VYR08_001205"
     CDS             complement(2722..4008)
                     /locus_tag="VYR08_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466733.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(4020..5666)
                     /locus_tag="VYR08_001206"
     CDS             complement(4020..5666)
                     /locus_tag="VYR08_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3466734.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(5668..6282)
                     /locus_tag="VYR08_001207"
     CDS             complement(5668..6282)
                     /locus_tag="VYR08_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV3466735.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            6417..6986
                     /locus_tag="VYR08_001208"
     CDS             6417..6986
                     /locus_tag="VYR08_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3466736.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(7016..7708)
                     /locus_tag="VYR08_001209"
     CDS             complement(7016..7708)
                     /locus_tag="VYR08_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466737.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7710..8771)
                     /locus_tag="VYR08_001210"
     CDS             complement(7710..8771)
                     /locus_tag="VYR08_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV3466738.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            complement(8764..10137)
                     /locus_tag="VYR08_001211"
     CDS             complement(8764..10137)
                     /locus_tag="VYR08_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV3466739.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEAPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDVKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(10237..11091)
                     /locus_tag="VYR08_001212"
     CDS             complement(10237..11091)
                     /locus_tag="VYR08_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV3466740.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
BASE COUNT         3181 a         2593 c         2075 g         3296 t
ORIGIN      
        1 attattctga aaattcaaaa aggagtattt atcatgtcgc aagctatttc cttaaatcaa
       61 tcaacctggg caagcaagct aaaagcaatg ggacctggaa tcctaatggc aactgccgct
      121 gttggaggtt cccacattgt atcctcaact caagctggcg gttcttacgg ttggtctcta
      181 cttctcttgg tcatcttagc caatgtcttt aaatatccat ttttccgttt tggtgctgaa
      241 tacacagctg atactggaaa gactttggtt gaaggttatg ccgaaaaagg aaaactctat
      301 ctctggattt tctttattct caatgtcttt tcggctatgg tcaacacggc tggtgttgcc
      361 attctgtgct cagctatcat cgccagtgcc ttcccaatga ttggacttag cattactcag
      421 tggtccctca ttctcgttgc aatcatttgg gctatgctac tctttggagg ctacaaactt
      481 ttagacggca tggtcaaatg gattatgtct gccttaacca ttgcgactgt tctggcagtt
      541 atcatagcag cggtcaagca tccagaatac agttctgatt ttgtcgagaa gacaccttgg
      601 caaatggcag ctctgccctt catcgtctcc ctcctaggat ggatgccggc tcctattgaa
      661 atttcagcca tcaattcact ttggtcagct gaaaagagaa agaccgtcaa ctttaacaca
      721 gaagacgctc tgtttgactt taacactggt tatattggaa cagctatcct agccgtcttc
      781 tttgtggcac tgggagcact gattcagtat cctacagggc aggcggttga agctgcttca
      841 gccaaataca tctctcaatt cgtgggcatg tatgcctctg ttcttggcga atggtcccgt
      901 tacttgatta cctttattgc cttcctctgt atctttggaa cagttataac tgttatcgat
      961 ggctattctc gcgttaatca ggaatctctc cgactgctaa tcagtcaaaa agaggacaat
     1021 cgtaaatctt tgaacatctg gatgaccatc actgctatca tcggtatcgt cattatcaag
     1081 ttcttcgctg gtcaggtttc aaccatgctc cgctttgcca tgattggatc tttcctgaca
     1141 acacctttct ttgctctttt gaattacgcc ttggtaacgc gtgaaaacaa aaatcttcct
     1201 tcttggctca aacaccttgc cattgcggga ttgattttcc tctttggctt cgccatcttc
     1261 tttatctacg cactcgcaat cggaaaagca gggtaaggga caagcgcgag atgaagataa
     1321 ggtttcattt caagagaaaa ttcagcaaat atttctatga taaaaagcat aagaacaagg
     1381 ttttgaagac ctgaacttat gcttttttac gttcttaaag actgtttata ctcaaaaaac
     1441 agttgaacaa cttcaaccac ctcttataag aactttatac tattcgagaa tctcttcaaa
     1501 ccacgtcagc tctatctgca acctcaaagc tgtgctttga gcaacctgcg actagcttcc
     1561 tagtttgctc tttgattttc attgagtatt aattctcctt ttccaactca tacaaatctg
     1621 cgataatagc tgcgacatgt ttgatatctt ccagcatgcc tcgcatttca aagtcagcca
     1681 atacagggaa gccaaagcgt tgactgtatt gcttggctgt taggcagtat tggttattaa
     1741 agttacgatt tcctgaccca accacaccaa aacacttact agcattgtta ccataggcaa
     1801 taaaatctcc caccggtgtc gtcaaaatct caacatctcc gttatccacg ccattcccac
     1861 cttcgagata ggtcggcaaa aaagcgacat agggatggtc catttcatag aaatttttgc
     1921 cttccttgac caaatccttg atatgaatct tttgaacctc aatccttttg tactgggaca
     1981 agagatagtc tttcaagcgc gtcacaaaac tttcagtgtt gccactcaaa ctaatataaa
     2041 ctaaagaaat tgtcttcata tgtacctcca tgattttatc tataatcaaa aaacaactac
     2101 caagattgta tcttgatagt tcttttttgt caatacttag gcaagctctt ctgtttcctc
     2161 ttcctctgcc aaggctttct gttgtcgcca catcttatag aagacaaggg ctaggaagag
     2221 aacattgatg acgatataga aaatgctaac agcttttgaa ccaatactca ctgtcaaacg
     2281 catgctctca tcttgaacca actgcacagc gtcttgtaaa ccaacatagc cataaatcga
     2341 accaataata gctaacaaaa cataaccaac gtaagtatag tttgcatatt gcaaattttt
     2401 acgaacaagg aagacaatcg ctacgaccac taaaatagta gatagcacaa tcaaagcaac
     2461 attaaaaata gaatgagatg cttctgctac tctataggta taattaagct ggtcttctaa
     2521 ttgctgagca ttgacccctg caacttgttt ctgagcagca cgaagggctt ctttactagg
     2581 caagggactc acaattccaa acagagatgg aactgaaata agggcagaca agattagcaa
     2641 gacccataga taaattggtt tctttttcat gatagaacct cctgataata ttatagcacc
     2701 ttttgtaaag gaatacaaat cttatagtat agtttctacc tgttttcggt aggcttttgg
     2761 cgattttccg cacaattttc gaaaaacttt atagaaatat ccaaggttac tgtaaccaac
     2821 agcataacaa atatcttcga tgctgtcact agttgaaagc aaaagttgtt gggcagcctt
     2881 aatacgttgt ttgtttagta actctgcaaa agtcgaatcg gtttcacgct taatcaactg
     2941 ccctagatag acaggattga taaagagagc cttactgata tccttgaggg aaagctcttt
     3001 ttggtaatca cgaccaatga cttccagcac actgaccaca ttttcattca tacggtattg
     3061 accgaaaaag ctgctcagag tttccttgat ataaggaacc aattcatcga aggattgaat
     3121 agcatgaatg gttttgacga tgtctgtcag gtcatcagct ttgagatgtt caaataaatg
     3181 gaaaacatcc atgacaaact gaataaaaag ctgtttagta atcggaacac gcggcgtatt
     3241 ttcaagaact accttctcca agagatttaa ctcttctacg atttgattga gatttccctg
     3301 aatgataacc ctataaatcg gttcgtaata ggcaaagaga ctctcagact gctgcagatt
     3361 tacagaaccg taaaagaggg ttttctcagc attcagcttc cagcgttcaa agtgttcttg
     3421 ataaggagct tcaaaaggtg taacgactaa accatctagg ggttggtcag aaatgaaaat
     3481 ctgccagtct tggcccaaga catagtaggg aatggcgaag gaaccttgtt tttccttgga
     3541 tagacctatc caccaattct ccttatcccc taaataacta acaaatccag cctcgtctaa
     3601 ttcttgacta agagtctgac ttttcttccc tctctcgccg agctgacctg caatcttctc
     3661 cagcagattt cccagctcta ccttatcaac aggcttgacc aaatagtcca ccacactaag
     3721 gttcattgct ctttttacat aatcaaactc ctgataacct gagagcagga tataggcagc
     3781 atctggtaag atctctttca tctcccgaat catatcaagc cctgttttgt ctggcatatt
     3841 gacatcggaa atgatgacat cgacaggatt ttcctgaaca tattctagag cttcatcggc
     3901 atgactggct gttgcgacga cctccatatc ccacttatca aagggaatca aacgcttcag
     3961 accttctgtc accatgtact catcatctac taataatact ttatacattt tctccctttc
     4021 tactcatctt gaattgtaat acgatactga acaccggctt gctctgcaga ctctatcgta
     4081 atggcatagc ggtctccaaa atagagcaca aaacgctcgt gtacattgac aatcccgata
     4141 gactgccttt gatcactgta gctggcttgg tgttcaaaat atctctgact taatttttct
     4201 cggatatttg ccaacttttc agccgacatt cctctaccat tatcgaccac caaaatttcc
     4261 acaaaaccat cctgtttaag agccttgatg ctaatcacat tatctgtccg cctgtggtca
     4321 acaccatgcg cgaaatagtt ttctaccagc ggttgcaagg taaacttggg aatcttcata
     4381 ttctctaact ctggatctat cttgaaacca taggcaatgg acttgggata gcgaaccatg
     4441 cagagatagc tgtatttacg gcaaaattct aattcctgtt tgaggagggt ctctctttcg
     4501 tcggaaatat tgttacgcaa gagactactg aattcataaa tgatatctgc caactcatct
     4561 tgactctgca taactgcata catgcgcaag aactccagcg tattatacat aaaatgagga
     4621 ttgatttgcg cctgcaaggc tcgcatattg gcatcttttt gactaagctc taactggtaa
     4681 atatcatgga tattcttttc caatcgatcc aacatatcat tggtcgtctc cgcgattagg
     4741 agtaattcct gatctttttc ggaaatgtca atccgacgcc cctcttcgcc ttgagcaatg
     4801 acttgaatgg attccactaa atccactacc tgcttttggt aattagcaaa agtctgcctc
     4861 aaagtcagat aaagaatgac aataaagaga aggctcaaac ccacaatcaa agcagagcta
     4921 gtcactgttc cttgtaaaac aaagttttta ggaactgcca cctgaacctg atatccatga
     4981 gaagttaagc caactagcca attttcactc tccctatcaa ctgtctcacc aatatgaaaa
     5041 atctccgtat caaaaggtga ggtcactgtt actgccatcg gagtatgacc tctagtatta
     5101 tcaatggcat ggtataaaac agcaggatcc aaggagatgt aaatcactcc taaatcttga
     5161 tctgacactg gatctgacac cggaatggca aaactatttc ctgctggttt gaaatcctca
     5221 gccctgattt tttctccact acgtttgtct ttagtagaaa catagacttc cttgtaatct
     5281 tgaaaagcaa tggccacccc agttacaaaa tcatttcgaa catagaggtc atcaacattt
     5341 tcatacagag agactgatat ataaggcgaa gctttccgct ctaattgcca gtaaaaatag
     5401 tctggcatgc taaggctaaa gtatttatag attccctcga tacgcgcttg attttcaact
     5461 aaagcctgag ctatctgggt tgactctcta tggtaatatt caatttcact cactgttcta
     5521 gcagttacac gctgggctac tctctgggct tccttttcac gtgaatccca gtcagcgtaa
     5581 gagagtaaga ttgcaaaact cgcaatgata atgatcatca ctgaactata ggttcttaga
     5641 agcgtatgta gccaaaactg cttcattcta ttttgtcgcc aatttttcat ggatgatttc
     5701 atagacaggt tccaacatat cactgataaa gaaatgccac atcccaattc ctacaaaaaa
     5761 tagcagggca ggcatataat aaccaattgc tacaagtagc acagtcccaa ggagaacctt
     5821 agccacagct gctagactca taaagatgcc aatcaaggat aatttgagac tatttcgata
     5881 ggacaaggca aaataaactt gtaattttaa agatactgta taagccaaaa agatcagggc
     5941 aactactagg acattcaaaa gggtcgcaat caaatgaaca atggtctgat gaggtaattg
     6001 caccaagaga tacaaaccat aggtcaaaac tagacccaca cctaaaaagc tatagaaaat
     6061 caggttgctt gagataaaat tttgcttgta aagagaccaa gcctccttca aactgtattc
     6121 ccgaaagcta taaccatgtt ctgcatataa gctcatcaag gtggcactag ccggcgctag
     6181 accaaggata atccctcctg ctaaagttaa tagccaaaag aaggctgtcg caatcatccc
     6241 taaaaagaaa cgattaaaga caaagtctaa aaatctaccc atgatattcc cctaaaaata
     6301 actatgaaac cattatatca tatttcaagc gttttcaaaa acttctagta tccatttaca
     6361 atcccaccaa accatggtaa aatgagaatt gtgaaaaaat tatcaggaga caattcatga
     6421 agaaatctat cttaactaca ctgctttttg cagttcttta cttcctctgc atggggattg
     6481 gtgtcctttt gggcaatctc tttgaccaaa ctggaaacat gttttatgcg cctgccttta
     6541 ctgcccttgt cggcggtagc gtctacatga tcctagtcgc aaaagttccg cgctttggag
     6601 ccattaccac tatcggcctt gtcattgccc tctttttctt gggaactaaa cacggtgctg
     6661 gttccttcct tcctggaatt atctgtggcc tcctagcaga tggagtagct catttaggaa
     6721 aatacaagga caaaacaaag aacttccttt ctttcattat tttcgccttt agtacaacag
     6781 gaccaatctt gcttatgtgg attgcgccca aagcctatat ggctactctt ctggcaagag
     6841 gaaaatccca agaatatatc gaccgtatca tggtcgctcc aaaccctgga actgtccttc
     6901 tatttatcgc aagtattgtc atcggagccc tagtgggtgc cttgattgga caagccttga
     6961 gtaaaaaatt tgcacagaaa atctgatcag ttaaaaagag ccacgcggct cttttttatt
     7021 tatggctcaa tttcttagtc aagaaatctc ccaagaattg gattgcaaag ataatcaaaa
     7081 tgataataat ggttgccaag atggtcacat cgtgattgta gcggttaaat ccataagcga
     7141 tggctacgtt accgatacca ccagctccaa ccgcaccggc catagctgtt tccccaacaa
     7201 gggaaatcaa ggtcacagtc gtcacacgga tcaaatctgg aagaccttct gataggtaaa
     7261 cacccacgat gtcccagaaa gtcgctccgc tcgcttgagc cgcctcaatg acaccgccat
     7321 ccaattcagc caagacaacc tgcacctgac gggcaaagaa ggcaaagact gcaaaagaaa
     7381 gtgggacaag ggctgcattt ggcccgatac ttgttttaac aatcaagtga gaaagtggtg
     7441 acaagattgc caagaggatg ataaagggaa ccgcacgaaa aattgaggta attttgtcta
     7501 aaatccagaa tacgacttta ttctccaaga caccacctgg cgctgtcaag acgagaaaga
     7561 gacctgccac tagccccaag aagcctccga taatgaagga aagaactgtc atataaagag
     7621 ttaagtagat agccgttccc cagcctgcct gaccagccca acccatttta tagacatttg
     7681 gtaaataggt ttgaatcaat gattccatct tactgtcctc ccttcaatac ttttagttgt
     7741 acacctgctt gacgaatggc ttcttgggca cctgccaacg ctgctttttc acctgacaag
     7801 accaccacca attctccaac aggagtacca tcgagaattt cgatattccc atagagaata
     7861 ttagccatta cttggtaatg cttgtacaat tcattcaaaa gtggctcgtc tgttgaagct
     7921 ccagcgtact tgagttgcac caagagactg ttttcagaca agtgttccac gatttcttgc
     7981 ttctcgattt tgaccatggc ttcgtcaata cctgtggctg ttgagataaa gtcttgagtc
     8041 aaaggttgtt tagggtttga gaagatttca aggacacttc cctcttcaat caaatgccca
     8101 tcctgcataa ccgccacacg gttggcaatg tctttgacaa tctgcatttc atgcgtaatc
     8161 aagacaactg tcaatcctaa tttttggttc aaatcttgca acaaggccaa aatctgcttg
     8221 gttgtcttag ggtcaagggc agaagttgac tcgtctgaaa tcaagatttt tggatcattg
     8281 gccaaggcac gcgcaattgc cacacgctgt ttttgccctc cagatagttg tgaagggtag
     8341 ttttcagcac gatctgccaa accaaccaag tccaacaact tagctacttt agccttcttt
     8401 tcttccttgc tgagtccaga gtgtttaagg gcaaaggcta cattctcctc tgctgtcttt
     8461 tggctcatca ggttaaaatg ctggaaaatc atcccgatat cttgacgttt acgacgcaac
     8521 tgctctgccg tcaaggtcac cttaccatca aaaatcacat cgtcgtcaat ggtaattttc
     8581 cctgcagatg gtttttgcaa gaggttaatc acccgtacaa gggttgattt ccctgctcca
     8641 gaatatccaa cgattccgta gatatcccct tcttggatgt gaatggtcac atccttaacc
     8701 gctgtgatgg ttctcttctt ttggtgaaaa gtcacatcga tctgatctaa cttgataata
     8761 tctctactca tagcttctaa tcagctcctc tactaattcg atatgggtat aataatcagc
     8821 gattcgcaca ttttcatctc caccgtggtc tcggctattg gcatttccta gaccgaatgc
     8881 aaccattggc acctctaggg catcaaagac cgtatgcata ggtcctgtcc ccgctgtcgt
     8941 cggcaagact gaaacgccct gtggatagaa tttcttggcc aactcgatca cattgagaat
     9001 ggctggtgcg ctcatatcgc ttcgatagct catctctccc aaggtatagt ataattctac
     9061 cttatcaaag ccatttttgt ctagctgttt ccgaattttt tccagaacat catgcggttc
     9121 tagacccgga accagacgaa cctctagctt ggcactggct tctgcaggca aaatagtctt
     9181 aacaccctga ccttgataac cagactggat tccttcgata ttaagcgctg gctcgaaaaa
     9241 gaaacgtttt agaaaggcca tccgctcctc ctgtaagaga ggcaactcca atccataaat
     9301 ccgactaact tcctctgggt ttcgttgacc ataagtttct agcaaggcca tttctcgttc
     9361 attgggctct tgtacttctt cgtacaagcc ttcaaccaag atacggccat ccgcagcacg
     9421 aagagactgt aaggcttgga ggagatacca aggagctgat tccacaacac caccataact
     9481 cgagtggata tccacatcag cgctttttac cttgacatca aaggtcacaa tccccttatt
     9541 gccaccagaa atttccagct gttccaaggc atttttggtc ccttgttccc agaccaacaa
     9601 atccgcccca cggagtttgt ctgcatgctt ttccaaatac ttatctaggt ctgttgaagc
     9661 cgattcctcc gctccctcca tgataaagct gatattgaca ggtaaatcat catggtgctg
     9721 catatatttt ctcaaagcac tcaagcgagc tgtgatatga cccttgtcgt catcaacccc
     9781 acgcccatac atgaagccat tgcggaccga aagcgtaaaa ggagcctctg tccagacctg
     9841 atccccatcc gctggcacag tgtcatagtg gttatagaaa atcaaggtct tggcatctgg
     9901 acgcgaactc ttgaaatgtg ccatgacaaa gggcgctgta tagctctcat caatctccac
     9961 ttcagctcca acacgcttga aaatctcacc cagataattt gcgacttcct tgagtccaac
    10021 ctgctgggca aagactgatt tcttagaaat caaggtacgc aaaacctcaa aataatgttg
    10081 gactacatga tccttttcaa atttttcaat ctgttcttgt tcgctaggaa aaaccatatt
    10141 ctctctacct ttctatgaac gaataatctt ctttacactc cgtgctctat acaaaagcaa
    10201 gaggtggaat tctctctccc acctccctgt ttcttattac caaactggtt gatccaaacc
    10261 gtctgatgtt tcttcgataa cttttttcac gtcatctgtg tggtaagctg cgattacttt
    10321 cttgatagca tcagccttag gtgatgtttc ccaatctttt ttcgcaacaa tgatgttgta
    10381 ccattgtttt gagttttcat cagcttgttc tttgaaaagt gctttcttgt agtccaattt
    10441 tgcttctgta acgaaggtat tgtttacaac ggcagcgtca actgatgaca atgaacgagc
    10501 tgtttggcta gcgtccaatt cagtgatttt caagttcttt ggattttctt tgatgttggc
    10561 aactgttgca agagcagttc cagaaacatc caatttaatc aagccagctg attgaagcaa
    10621 gtaaagcgca cggctttcgt ttgtagcgtc attcggtaca gcgatttctc cgtttgctgg
    10681 gatgtcttct actttagtgt acttgttggc acttccattc aaacctgagt aaaggcggat
    10741 tggagagatg taagtatctc caatcgctac aaggtctttt ccgttttctt tgttccagtt
    10801 gttcaagaag ttatagtgtt ggaaagcgtt caaatctact tcgccatcag cagttgcttt
    10861 gtttggttgt gagtagtctg tgaactctgt aaattccaag gtaattccgt cttttttaac
    10921 caattcttgg attttgtccc aacgtttttc ttcagaaccg ctacggttaa cagttgcgat
    10981 tttgatagtt gttgcattgt ctgctttctt ttctgagttt ccgcaagctg caagagccaa
    11041 acctgcgact gtagcaaggg ctgctagacc aagccatttt ttgattttca tgattctttc
    11101 tccttaaaaa taataccgta ctagtatctc acaatttgtt tgatt
//
LOCUS       DAVMHG010000058        11123 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMHG010000058 DAVMHG010000000
VERSION     DAVMHG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.058"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            253..1653
                     /locus_tag="VYR08_001213"
     CDS             253..1653
                     /locus_tag="VYR08_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV3466741.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            1655..2014
                     /locus_tag="VYR08_001214"
     CDS             1655..2014
                     /locus_tag="VYR08_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV3466742.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            2068..2817
                     /locus_tag="VYR08_001215"
     CDS             2068..2817
                     /locus_tag="VYR08_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV3466743.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            2807..3106
                     /locus_tag="VYR08_001216"
     CDS             2807..3106
                     /locus_tag="VYR08_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV3466744.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            3177..4112
                     /locus_tag="VYR08_001217"
     CDS             3177..4112
                     /locus_tag="VYR08_001217"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV3466745.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            4198..4710
                     /locus_tag="VYR08_001218"
     CDS             4198..4710
                     /locus_tag="VYR08_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV3466746.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            4747..5343
                     /locus_tag="VYR08_001219"
                     /pseudo
     CDS             4747..5343
                     /locus_tag="VYR08_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            5444..5638
                     /locus_tag="VYR08_001220"
     CDS             5444..5638
                     /locus_tag="VYR08_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3466747.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(5684..7129)
                     /locus_tag="VYR08_001221"
     CDS             complement(5684..7129)
                     /locus_tag="VYR08_001221"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV3466748.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            7235..8857
                     /locus_tag="VYR08_001222"
     CDS             7235..8857
                     /locus_tag="VYR08_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV3466749.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            9060..11018
                     /locus_tag="VYR08_001223"
     CDS             9060..11018
                     /locus_tag="VYR08_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466750.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT         3278 a         2044 c         2472 g         3329 t
ORIGIN      
        1 aagaaagcga attaagaccc caacgggtct tttttcacaa gctttgaaat gagagagcga
       61 gttaggctca gtggctccct ttttacgagt ttgaaaacaa gagaacacat taaaagcacg
      121 atggagcttt ttcataaata tgtctctttt ctcatccaag aaattaaaat aaaaatgata
      181 aactttttga aaatagggga tttgcctact ttatgatata atggaaagag ttagattgaa
      241 agaggtagtg agatggacgc gaaattaaga tacaaggcaa agaagatcaa gattgtcttt
      301 tttgatattg atgatacatt gcggaattca aagacagggt ttattccaac tacaatcccc
      361 actgttttta aacagttgcg tgaaaaagga attttaacag gaatcgcctc tggacgtggc
      421 atttttggtg ttgttccaga gattcgtgat ctcaagcctg acttttttgt aactttgaat
      481 ggggcttata tcgaagataa aaaaggtcag gtcatttatc agcatcagat tgaaaagtca
      541 gatgttgagg agtatatctc ttgggctaag caagaaggaa ttgagtatgg cttggttggg
      601 agtcatgatg ccaagttgtc gactcgcacc gatatgatga gtgaagctat caatccaatt
      661 tatcccgact tagatgtaga tcccgatttc catgaaaaag aagatatcta tcagatgtgg
      721 acttttgaag ataagggaga tgacttgcac ttgcctgaca gtctctcaga caaacttcgt
      781 atggttcgtt ggcatcaaca ttcgtctgat attgtgccga tttcaggatc caaagcgacg
      841 ggggtggaaa aggttgtgga acaccttggc ttgaaaccag agaaggtcat ggtttttgga
      901 gatggtctca acgacttgga actctttgat tatgctggaa tcagcgttgc catgggaatt
      961 tctcatgata aaatcaaaga aaaagcagat tatattacaa aaacattaga agaagatggc
     1021 atttttgctg ccttagaagt atttggtatg gtagaaaaag aattgcattt tccacaagta
     1081 gacattgaaa cagtagaagg tcctcttgcg actattaaga ccaatcacgg agacttacgt
     1141 atcaagcttt tccctgaaca tgctcctaaa acagtggcta actttgtatc tctttcaaaa
     1201 gatggctact atgatggtgt cattttccac cgtattatca aggactttat gatccaaggt
     1261 ggagacccaa ctggaactgg tatgggtggc cagtcaatct acggcgaatc ttttgaggat
     1321 gaattctcag aagagcttta caatatccgt ggtgctcttt ccatggcaaa tgctggtcca
     1381 aataccaacg gcagccagtt ctttatcgtg caaaaccaac acctacctta ttctaagaaa
     1441 gaaattactc gtggtggttg gccagaaccg attgcagaaa tctatgccaa tcaaggtggg
     1501 acacctcacc tagaccgccg tcacacggtt tttggtcagt tagctgatga agcatcttac
     1561 gctgtcttgg atgccattgc tgctgttgag acaggagcta tggacaagcc agttgaagat
     1621 gttgtaattg aaactattga aatcgaggac taagatgaaa atcggtgata agctaaaggg
     1681 gcgtattaca gggattcagc cctacggtgc ctttgttgag ttagagacgg gtgatacggg
     1741 gctgattcac atttcagaaa ttcggacagg atttattgaa aatattcatg aaaccttgaa
     1801 agtcgatgaa gaggttcaag ttcaggtagt ggatttagat gaatttacgg ggaaagccag
     1861 tctttctatc cgcactttgg aggaagaaaa gtaccagttt cctagacgga gacgcttttc
     1921 aagcgaccgc tttaactacg gttttgctcc ctttcgacga atgctaccaa tctggactgg
     1981 agaagccctg caccatttaa aaaagaagaa gtagtttaga gttttatatt ttttgtaatg
     2041 ttaatatgaa tttgctataa taggaccatg gaagaagaac aattattaaa atcaggagag
     2101 cgcattaacc agctcttttc gacagatatc aaaatcattc aaaatagaga ggtttttagc
     2161 tattcggtgg atagtgttct cttatcacga tttccacgtt ttcctaagaa ggggttgatt
     2221 gtggatttct gtgctgggaa tggagcagtg gggctttttg ctagcactcg tactcaagca
     2281 cagatattgt ctgttgagat tcaggagcgt ttggcggata tggctgaacg ctctgtccgt
     2341 ttgaatggat tagaggagca aatgcaggtc atctgcgatg atttgaaaaa tatgcctgct
     2401 cacattcagg ggagtaaggt ggatatgatt ttgtgtaatc caccctattt caaggtggat
     2461 ccttattcta atctgaacga gagtgaacat tatctcttgg ctcgacacga aatcacgact
     2521 aatttggaag aaatctgtcg tagtgcccag agtattctca agtctaatgg gcgtttggcc
     2581 atggttcatc gtcctgatcg acttctggat attttggata cgttaaaacg acataatcta
     2641 gcccctaagc gcctgcagtt tgtttatcca aaaagagaaa aggaagccaa tatgcttttg
     2701 attgaggcga tcaaggatgg ctcaacaagt ggctttaagg tcttacctcc tttgattgtc
     2761 cacaatgatg atggctctta tacgcccgaa atcgaagaga tttattatgg atcataaggc
     2821 ttatatgtac gttctggagt gtcgtgatgg atcctactat ataggctata cgactgatat
     2881 gagaagacgc ctcgctatcc acaatagtgg gaagggagcc aaatatacac gagcacgctt
     2941 gccagtcaaa cttatctatg ctcaaggttt tgccagtaag gaagaagcca tgtcggctga
     3001 agctcttctc aagcgtaaga agaggccaca gaaggaagaa tttttatctg aaaatcaaga
     3061 tagaaattta ctcagttatt ttgaagaatc gtggggagtc ctttgactcc tcttaccttt
     3121 gtcaaaaagc gaaaaaaatg ctacaataaa gaaaataaac aaaatgaggt attatcatgt
     3181 ccaagattct agtatttggt caccaaaatc cagactcaga tgccatcgga tcatctgtag
     3241 cttttgccta ccttgcaaaa gaagcttacg gtttggatac ggaagctgtt gcccttggaa
     3301 ctccaaatga agaaacagcc tttgtcttga actattttgg tgtggaagca ccacgtgtta
     3361 tcacttctgc caaagcagag ggggcagagc aagttatctt gactgaccac aatgaattcc
     3421 aacaatctgt atcagatatc gctgaagtag aagtttacgg tgttgtagac caccaccgtg
     3481 tggctaactt tgaaactgca agcccacttt acatgcgttt ggagccagtt ggatcagcgt
     3541 cttcaatcgt ttaccgtatg ttcaaagaac atggtgtagc tgtgcctaaa gagattgcag
     3601 gtttgatgct ttcaggtttg atttcagata cccttctttt gaaatcacca acaacacacc
     3661 caacagataa aatcattgct cctgaattgg ctgaattggc tggtgtgaac ttggaagaat
     3721 atggtttggc aatgttgaaa gctggtacca acttggctag caaatctgct gaagaattga
     3781 ttgatatcga tgctaagact tttgaactca acggaaataa tgtccgtgtt gcccaagtga
     3841 acacagttga catcgctgaa gttttggaac gccaagcaga aattgaagct gcaatgcaag
     3901 ctgccaacga atcaaacggc tactctgact ttgtcttgat gattacagat atcgtcaact
     3961 caaactcaga aatcttggct cttggtgcca atatggacaa ggtcgaagcg gctttcaact
     4021 tcaaacttga aaacaatcat gccttccttg ctggtgccgt ttcacgtaag aaacaagtgg
     4081 tacctcaatt gactgaaagc tttaatgcgt aagattttgg gtgtcagctc aaaatcggaa
     4141 agtctagttt gccttatatc gaaaggagtt tcggctcctt ttttctagga gtgaagtatg
     4201 ttagaaaatg gcgatttgat ttttgtgaga gatgggtcag acatgggaca ggccatccag
     4261 acttccacag gtaactatag ccatgttgcc atttatttgg atgggatgat ttatcatgct
     4321 agtggacagg ctggtgttgt ctgtcaagaa ccggcagact tctttgagtc caatcattta
     4381 tacgacctct atgtttaccc agaaatggat atccagtcgg tgaaggaaag agcttgcaaa
     4441 catcttggag caccctacaa tgcttctttc tatccagatg cagctggttt ttactgctcc
     4501 cagtatatag cagaaatcct acctattttt gaaactattc ctatgaaatt tggagatggg
     4561 gagcaggaga ttagtgattt ttggagggag tattacatag aactaggtct gcctgttcct
     4621 ctgaaccaag ctggtaccaa tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa
     4681 gaaaggaatc ttcatgattc agatttttaa tccatctcgt ttgacgagac agccattttt
     4741 ggagaattga tccgctatct ggatcagtat gaggatgtga ttctacggga aattaaggct
     4801 caatttccag atgttgcagt tgataaactc atggaagagt atataaaggc aggcttgatt
     4861 ctacgtgaaa ataagcgcta ttacctcaat tttcctacgc ttgaatcact tgatagtctt
     4921 gaactggatc aagagatttt tgtcagagaa gctagtccgg tctatcaagc cttgttggag
     4981 cagagttttg agacggaatt gcgcaatcaa atcaatgcag ctattttagt tgaaaagacg
     5041 gactttgcgc gcattaaaat gaccctgtcc aattattttt acaaggtcaa acagcagtat
     5101 cctttgacag aaaaacagca ggagctctat gacattttag gagatgttaa tcctgagtat
     5161 gccctcaagt atatgacggc ttttttgttg aaatttctca aaaaagacca gcttatgcag
     5221 aaatgccgtg atatctttgt ggacagttag gttgtcttag gctatattgt gcaaaatgaa
     5281 gatggaaagt atgagttggc tatcgatttt gataaggaga ggttaacttt ctacttagcg
     5341 tgatttcttg tttctgagta cattgtttga ctttccttag tattcggtat aaactatatg
     5401 taaccggtaa cacatatcgg aataaactaa aggagacaat catatgtcac ttgaaaacaa
     5461 attggaacaa gcaacaggcg ctgtcaaaga aggttttggt aaagttactg gagacagcaa
     5521 gacagaactt gaaggagctg ttgaaaaaac agtttctaag gcaaaagacg ttgtagaaga
     5581 cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa aacgttttta ctaaataata
     5641 ggaaaaaatc aagggtttca ttttcccttg attttttcta ttcttataaa taattttctg
     5701 cgacggctgt atctcctggg taggattctt tcttgccctg gatgatttgg taacaatcgg
     5761 ctcccttacc cgcaataata actgcatcta attcgtgatt tgtgatagcc attgccgcct
     5821 tgatggcttc ttggcgatcc gcaatcttct caacaggatg attgatgtag ctactaattt
     5881 catctgcaat ggccattggg tcttcatagt tagggtcatc agcagtcaga aagacttgaa
     5941 tctcagggtg ttgattgagg aggaggccaa agtccttacg acgactttct cccttgtttc
     6001 ctgttgatcc cagaaccaga gcaatctttc cggtttgatg agtttcaacc acattgatga
     6061 gttttttcag actatcccca ttgtgggcat agtcgatgaa taccttggct ccatttttct
     6121 gagtgaggac ttccatacga ccaggaacgc gggttgcagc gatgcctttt ttgatgtcct
     6181 caagacttgc tccgagacgg agacaagcaa gtccagcagc aactgcattt tcttggttga
     6241 agttgccaat gagttggata tcataatctc cagcgagttt acccgtagct gaaaatctaa
     6301 aggctttgga attctcgatt tggttatcaa attggctacc atagaaatca tggtcttgat
     6361 cttcaacctg ttctttcaag actgagaagt ggtccatgtc actgttaatg atgactgctc
     6421 ggctattttc catcaagaga cgcttgtggt agaaatagtc ttcaaagcta gggtgttcaa
     6481 tcgggccgat atggtctagg ctgatattta ggaaaactcc cacatcaaag gttagaccat
     6541 agacacgttt gaccagatag gcttgactgg agacttccat gatgaggtgg gtacggtcat
     6601 tttgcacagc ctgattcatc atgtcaaaga ggtcaatact ctcaggggtt gtcaacgctg
     6661 acttaaagaa agtctctcca tcaagagttg tgttcatggt cgacaacata gcaggtcgat
     6721 gcccttgaga taagatgtta taggcgaaat aggctgctgt tgtcttaccc ttagtaccag
     6781 taaaggcaag gagtttgagt ttttcctgtg gattaccata gaactccatg gcaatcaaac
     6841 tcatggcttt ctttatatcg ttcacaatga tgacagggat accgacttcg tagtcctttt
     6901 cagctatata ccaagctaag ccttgtgtta tagcagaaag aaggtattct tttttaaagg
     6961 cagcgccttt tgcaaaaaaa agagtgtctt ctgttacttt tcggctgtcg tagctgatgc
     7021 tatcaaaaac aactttgctg tagttgtagt ggtaatgacc ttggtcaata atttcgcgaa
     7081 aaaggccatc tttctttaaa atatctaata cagtttcaat ttttatcata ctttctattg
     7141 taaaccgaaa gtcgcaaatt tacaagtaac aaggaaaagt ttataatgga agataaggag
     7201 tttttcctag ttatcaaaat tgaatgagga atctatgtcg cacgaaaaca atcaccagca
     7261 ggcccagatg ttacggggga ctgcttggct aacggctagt aactttatca gtcgcctact
     7321 cggggctgtt tacattatcc cttggtacat ctggatgggg gcttatgcag ctaaggcaaa
     7381 tggtctcttt accatgggtt acaatatcta tgcttggttc ttgttggttt caacagcggg
     7441 gattccagtt gcggtggcca agcaagttgc caagtataat accatgcgag aagaagagca
     7501 tagctttgcc ctgattcgga gcttcttagg ctttatgaca ggactaggcc tggtttttgc
     7561 tttagtcttg tatgtctttg ctccttggct agcagacttg tctggcgtgg gcaaagactt
     7621 gatcccaatc atgcaaagct tggcttgggg agtcttgatt ttcccgtcta tgagtgttat
     7681 ccgaggattt ttccaaggga tgaataacct caaaccctat gccatgagcc aaattgctga
     7741 gcaggtcatt cgtgttatct ggatgctcct agcaaccttt atcattatga agctcggttc
     7801 aggagattat ctagcagccg ttacccaatc aacctttgct gcctttgtcg gtatggtagc
     7861 cagttttgca gtcttgattt atttccttgc ccaagaaggt tcactcaaaa gagtctttga
     7921 aacaggagat aagattaaca gtaagcgtct cttggttgat accattaagg aagccattcc
     7981 ttttatcctg acagggtctg ccatccagct cttccagatt ttggatcagc tgacctttat
     8041 caatagtatg agctggttta ccaactacag caatgaggac ttggttgtca tgttttctta
     8101 tttctcagcc aatcctaata aaatcacgat gattttgatt tctgtagggg tttcgattgg
     8161 gagtgttggt ttgccacttt tgacggaaaa ctatgtcaag ggggacttga aagcagcttc
     8221 tcgtctcgtt caggacagtc tcaccctact ctttatgttc ttgctaccag caacggttgg
     8281 agtggttatg gtaggagaac ctctttatac ggtcttctat ggtaagccag atagtttggc
     8341 tctgggctta tttgtctttg cagttttgca gtctattatt ttaggcttgt acatggtctt
     8401 gtctccaatg cttcaggcca tgttccgcaa ccgcaaggcc gttctctatt ttatctatgg
     8461 ttctattgcc aagctagtct tgcaactacc taccatcgcc ctcttccaca gttatggtcc
     8521 tttgatttca acaaccattg ctctcatcat tcctaacgtc ttgatgtatc gggatatttg
     8581 taaagtaact ggtgtcaagc gcaaggtgat tttgaagcga accattttaa tcagtttgct
     8641 gaccctagtc atgtttctgt taataggaac catccagtgg ctgttaggat ttttcttcca
     8701 accaagtgga cgtttgtgga gcttctttta tgtagctctt gtcggtgcca tggggggtgg
     8761 actttatatg gttatgagtc tgcgtaccta tttattagat aaggtaatag gaaaagccca
     8821 agcagatcgc ctgcgagcaa aatttaagct ttcgtaaggt agcatttata aataaaagga
     8881 ataagttgaa caatctttta ggagaacttg aaacttattc cttttttagc gtcaaaatag
     8941 ctaaaattat ctttcaaaaa tagaaaatga ggtttttttg attttttgga aaaaacaatt
     9001 gactaaacaa accaagagtt gtataataaa acaaatattt aaagcaggag gttctggaaa
     9061 tgaaaaagtc taagagcaaa tatctaacct tggcaggtct tgtcctgggt acaggagttt
     9121 tattgagcgc gtgtggaaat tctagcactg catcaaaaac ctataactat gtttattcaa
     9181 gtgatccatc tagtttgaac tatctagcag aaaaccgcgc agcaacatcc gatattgttg
     9241 caaatttggt agacgggtta ttagaaaatg accaatatgg gaatattatt ccatcattag
     9301 cagaggattg gactgtttct caggacggtt tgacctatac ctacaaactt cgtaaggatg
     9361 ccaagtggtt tacttctgag ggagaagaat atgcgcctgt aactgcccag gattttgtga
     9421 caggtttgca atatgcagct gataaaaaat cagaagcttt gtatctagtg caggactctg
     9481 ttgctggttt ggatgactat atcactggta aaacaagcga cttttcaact gtcggtgtca
     9541 aggcacttga tgaccaaacg gttcaatata ctttggttaa accagaactt tactggaatt
     9601 caaaaacact tgcaacgata ctttttcctg ttaatgcaga tttcctgaaa tcaaaagggg
     9661 atgattttgg gaaggcggat ccatctagta ttttgtacaa tggacctttc ttgatgaaag
     9721 cacttgtctc aaaatctgct attgaatata agaaaaaccc taattactgg gatgctaaga
     9781 atgtctttgt agacgatgtg aaattgacct actatgatgg tagcgaccaa gaatcactgg
     9841 aacgtaattt tacagctggt gcttatacta cggctcgtct ttttcctaac agctccagct
     9901 atgaagggat taaagaaaaa tacaaaaaca atatcatcta tagtatgcaa aattcaactt
     9961 catatttctt taattttaac ctagatagga agtcttacaa ttatacttct aaaacaagtg
    10021 acattgaaaa gaaatcgacc caggaagcag ttctcaataa aaacttccgt caggctatca
    10081 attttgcttt tgacagaaca tcttatgggg ctcagtctga agggaaagaa ggtgcaacaa
    10141 agattttgcg taacctagtg gttcctccaa actttgtcag tatcaaggga aaagactttg
    10201 gtgaagttgt agcctctaag atggtcaact atggtaagga atggcaaggt atcaactttg
    10261 cggatggtca agacccttat tacaatcctg agaaagccaa ggctaagttt gcggaagcta
    10321 agaaagaact cgaagcaaag ggtgttcaat tcccaatcca cttggataag actgtggaag
    10381 taacagataa agtaggcata caaggagtta gttctatcaa acaatcaatt gaatctgttt
    10441 taggttctga taatgtagtg attgacattc agcaattaac atcagatgag tttgacagtt
    10501 caggctactt tgctcaaaca gctgctcaga aagactatga tttatatcat ggcggttggg
    10561 gacctgatta tcaagacccg tcaacctatc tcgatatttt taatactaat agtggaggag
    10621 ttctacaaaa tcttggacta gagcctggag aagccaatga caaggctaag gcagttggac
    10681 tggatgtcta tactcaaatg ttggaagaag ctaataaaga gcaagatcca gccaaacgtt
    10741 atgagaaata tgctgatatt caagcttggt tgattgatag ttctttagtt cttccaagtg
    10801 tttcgcgtgg gggaacacca tcattgagaa gaaccgtacc atttgctgct gcctatggtt
    10861 taaccggtac aaaaggggtt gaatcatata aatacctcaa agtacaagat aagattgtca
    10921 caacagacga atatgcaaaa gccagagaaa aatggttgaa agaaaaagaa gaatccaata
    10981 aaaaagccca agaagaattg gcaaaacatg tcaaataaaa tctaattgta gataagtttg
    11041 tgtaagaagt ctttgtgctt aaaaacgcat aatatcaggt atataaaatc ctagatgtta
    11101 tgcgttttgt tataggaaga ttt
//
LOCUS       DAVMHG010000059        11040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMHG010000059 DAVMHG010000000
VERSION     DAVMHG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.059"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..1999
                     /locus_tag="VYR08_001224"
     CDS             17..1999
                     /locus_tag="VYR08_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3466751.1"
                     /translation="MKSSKLFALAGVTLLVATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYAIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2300..7603
                     /locus_tag="VYR08_001225"
     CDS             2300..7603
                     /locus_tag="VYR08_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV3466752.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDNERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDESKLLSSISF
                     LGNALVSVSSNQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREASDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLDAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALILASVSLALSALFVVKTKKD"
     gene            complement(7770..9929)
                     /locus_tag="VYR08_001226"
     CDS             complement(7770..9929)
                     /locus_tag="VYR08_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV3466753.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(9926..10522)
                     /gene="recU"
                     /locus_tag="VYR08_001227"
     CDS             complement(9926..10522)
                     /gene="recU"
                     /locus_tag="VYR08_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV3466754.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10588..>11040
                     /locus_tag="VYR08_001228"
     CDS             10588..>11040
                     /locus_tag="VYR08_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HEV3466755.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKN"
BASE COUNT         3404 a         2051 c         2478 g         3107 t
ORIGIN      
        1 tttaaaggag aaaatgatga aaagttcaaa actatttgcc cttgcgggcg tgacattatt
       61 ggtggcgact actttagctg catgctctgg atcaggttca agcactaaag gtgagaagac
      121 attctcatac atttatgaga cagaccctga taacctcaac tatttgacaa ctgctaaggc
      181 tgcgacagca aatattacca gtaacgtggt tgatggtttg ctagaaaatg atcgctacgg
      241 gaactttgtg ccgtctatgg ctgaggattg gtctgtatcc aaggatggat tgacttacac
      301 ttatgctatc cgtaaggatg caaaatggta tacttctgaa ggtgaagaat acgcggcagt
      361 caaagctcaa gactttgtaa caggactaaa atatgctgct gataaaaaat cagatgctct
      421 ttaccttgtt caagaatcaa tcaaagggtt ggatgcctat gtaaaagggg aaatcaaaga
      481 tttctcacaa gtaggaatta aggctctgga tgaacagaca gttcagtaca ctttgaacaa
      541 accagaaagc ttctggaatt ctaagacaac catgggtgtg cttgcgccag ttaatgaaga
      601 gtttttgaat tcaaaaggag atgattttgc caaagctacg gatccaagta gtctcttgta
      661 taacggtcct tatttgttga aatccattgt gaccaaatct tctgttgaat ttgcgaaaaa
      721 tccgaactac tgggataagg acaatgtgca tattgacaaa gttaaattgt cattctggga
      781 tggtcaagat accagcaaac ctgcagaaaa ctttaaagat ggtagcctta cagcagctcg
      841 tctctatcca acaagtgcaa gtttcgcaga gcttgagaag agtatgaagg acaatattgt
      901 ctatactcaa caagactcta ttacgtatct agtcggtaca aatattgacc gtcagtccta
      961 taaatacaca tctaagacca gcgatgaaca aaaggcatcg actaaaaagg ctctcttaaa
     1021 caaggatttc cgtcaggcta ttgcctttgg ttttgatcgt acagcctatg cctctcagtt
     1081 gaatggacaa actggagcaa gcaaaatctt acgtaatatc tttgttccac caacatttgt
     1141 tcaagcagat ggtaaaaact ttggcgatat ggtcaaagag aaattggtca cttatgggga
     1201 tgaatggaag gatgttaatc ttgcagattc tcaggatggt ctttacaatc cagaaaaagc
     1261 caaggctgaa tttgctaaag ctaaatcagc cttacaagca gaaggtgtga cattcccaat
     1321 tcatttggat atgccagttg accaaacagc aactacaaaa gttcagcgcg tccaatctat
     1381 gaaacaatcc ttggaagcaa ctttaggagc tgataatgtc attattgata tccaacaact
     1441 acaaaaagac gaagtaaaca atattacata ttttgctgaa aatgctgctg gcgaagactg
     1501 ggatttatca gataatgtcg gttggggtcc agactttgcc gatccatcaa cctaccttga
     1561 tattatcaaa ccttctgtag gagaaagtac taaaacatat ttagggtttg actcagggga
     1621 agataatgta gctgctaaaa aagtaggtct atatgactac gaaaaattgg ttactgaggc
     1681 tggtgatgag gctacagatg ttgctaaacg ctatgataaa tacgctgcag cccaagcttg
     1741 gttgacagat agtgctttga ttattccaac tacatctcgt acagggcgtc caatcttgtc
     1801 taagatggta ccatttacaa taccatttgc attgtcagga aataaaggta caagtgaacc
     1861 agtcttatat aaatacttgg aacttcaaga caaggcagtc actgtagatg aataccaaaa
     1921 agctcaggaa aaatggatga aagaaaaaga agagtctaat aaaaaggctc aagaagatct
     1981 cgcaaaacat gtgaaataac tgttgcaaaa tataagaaag gatttagtat ttctcttgaa
     2041 tgctgaatcc ttttttacat ttgtaaagaa agattctaaa tgtactgacc ctcaaaagtt
     2101 ggagcctctt tttgtcagaa tagagaaaat ttttgttaat tttacttgtt tcctattgct
     2161 ttctcagcta ttatttgtta tattaaaagt ataattattt tttatttatc agggttaagc
     2221 attgcacttt cagaggaagg agtatttttt aaaaaggaaa tgtaaacgct tgctcaaaaa
     2281 tgaaaggatt tagaagttta tgaataaagg attatttgaa aaacgttgta aatatagtat
     2341 tcggaaattt tcattaggtg ttgcttctgt tatgattgga gctgcattct ttgggacaag
     2401 tccggttctt gcagatagcg tgcagtctgg ttccacggcg aatttaccag ctgatttagc
     2461 tactgctctt gcaacagcaa aagagaatga tgggcgtgat tttgaagcgc ctaaggtggg
     2521 agaagaccaa ggttctccag aagttacaga tggacctaag acagaagaag aactattagc
     2581 acttgaaaaa gaaaaaccgg ctgaagaaaa accaaaagag gataaacctg cagctgctaa
     2641 acctgaaaca cctaagacgg taacccctga atggcaaacg gtagagaaaa aagaacaaca
     2701 gggaacagtc actatccgag aagaaaaagg tgtccgctac aaccaattat cctcaactgc
     2761 tcaaaatgat aacgcaggta aaccagccct gtttgaaaag aagggcttga ccgttgatgc
     2821 caatggaaat gcaactgttg atttaacctt caaagatgat tctgaaaagg gcaaatcacg
     2881 ctttggtgtc ttcttgaaat ttaaagatac caagaataat gtttttgtcg gttatgacaa
     2941 ggatggctgg ttctgggagt ataaatctcc aacaactagc acttggtata gaggtagtcg
     3001 tgttgctgct cctgaaacag gatcaacaaa ccgtctctct atcactctca agtcagacgg
     3061 tcagctaaat gccagcaata atgatgtcaa tctctttgac acagtgactc taccagctgc
     3121 ggtcaatgac catcttaaaa atgagaagaa gattcttctc aaggcgggct cttatgacaa
     3181 tgagcgaaca gttgttagcg ttaaaacgga taaccaagag ggggtaaaaa cagaggatac
     3241 ccctgctgaa aaagaaacag gtcctgaagt tgatgatagc aaggtgactt atgacacgat
     3301 tcagtctaag gtcctcaaag cagtgattga ccaagccttc cctcgtgtca aggaatacag
     3361 cttgaacggg catactttgc caggacaggt gcaacagttc aaccaagtct ttatcaataa
     3421 ccaccgaatc acccctgaag tcacttataa gaaaatcaat gagacaacag cagagtactt
     3481 gatgaagctt cgcgatgatg ctcacttaat caatgcggaa atgacagtac gcttgcaagt
     3541 tgtagacaat caattgcact ttgatgtgac taagattgtc aaccacaatc aagtcactcc
     3601 aggtcaaaag attgatgacg aaagcaaact actttcttct attagtttcc tcggcaatgc
     3661 tttagtctct gtttctagta atcaaactgg tgctaagttt gatggggcaa ccatgtcaaa
     3721 caatacgcat gtcagcggag atgatcatat cgatgtaacc aatccaatga aggatttggc
     3781 taagggttac atgtatggat ttgtttctac agataagctt gctgctggtg tttggagtaa
     3841 ctctcaaaac agctatggtg gtggttcgaa tgactggact cgtttgacag cttataaaga
     3901 aacagtcgga aatgccaact atgtaggaat ccacagctct gaatggcaat gggaaaaagc
     3961 ttataagggt attgttttcc cagaatacac gaaggaactt ccaagtgcta aggttgttat
     4021 cactgaagat gccaatgcag acaagaaagt tgattggcaa gatggtgcca ttgcttatcg
     4081 tagcattatg aacaaccctc aaggttggga aaaagttaag gatatcacag cttaccgtat
     4141 cgcgatgaac tttggttctc aagcacaaaa cccattcctt atgaccttgg atggtatcaa
     4201 gaaaatcaat ctccacacag atggtcttgg gcaaggtgtt ctccttaaag gatatggtag
     4261 cgaaggtcat gactctggtc acttgaacta tgctgatatt ggtaagcgta tcggtggtgt
     4321 cgaagacttc aagaccctaa ttgagaaggc taagaaatat ggagctcatc taggtatcca
     4381 cgttaacgct tcagaaactt atcctgagtc taaatacttc aatgaaaaaa ttctccgtaa
     4441 gaatccagat ggaagctata gctatggttg gaactggcta gatcaaggta tcaacattga
     4501 tgctgcctat gacctagctc atggtcgttt ggcacgttgg gaagatttga agaaaaaact
     4561 tggtgacggt ctcgacttta tctatgtgga cgtttggggt aatggtcaat caggtgataa
     4621 cggtgcctgg gctacccacg ttcttgctaa agaaattaac aaacaaggct ggcgctttgc
     4681 gatcgagtgg ggccatggtg gtgagtacga ctctaccttc catcactggg cagctgactt
     4741 gacctacggt ggctacacca ataaaggtat caacagtgcc atcacccgct ttatccgtaa
     4801 ccaccaaaaa gatgcttggg ttggggacta cagaagttat ggtggtgcag ccaactatcc
     4861 actgctaggt ggctacagca tgaaagactt tgaaggctgg caaggaagaa gtgactacaa
     4921 tggctatgta accaacttat ttgcccatga cgtcatgact aagtacttcc aacacttcac
     4981 tgtaagtaaa tgggaaaatg gtacaccggt gactatgacc gataacggta gcacctataa
     5041 atggactcca gaaatgcgag tggaattggt agatgctgac aataataaag tagttgtaac
     5101 tcgtaagtca aatgatgtca atagcccaca atatcgcgaa cgtacagtaa ctctcaacgg
     5161 acgtgtcatc caagatggtt cagcttactt gactccttgg aactgggatg caaatggtaa
     5221 gaaactttct actgataagg aaaagatgta ctacttcaat acgcaggccg gtgcaacaac
     5281 ttggaccctt ccaagcgatt gggcaaagag caaggtttac ctttacaagc taactgacca
     5341 aggtaagaca gaagagcaag aactaactgt aaaagatggt aaaattaccc tagatcttct
     5401 agcaaatcaa ccatatgttc tctatcgttc gaaacaaact aatcctgaaa tgtcatggag
     5461 tgaaggcatg cacatctatg accaaggatt taatagcggt accttgaaac attggaccat
     5521 ttcaggcgat gcttctaagg cagaaattgt caagtctcaa ggggcaaacg atatgcttcg
     5581 tattcaagga aacaaagaaa aagttagtct cactcagaaa ttaactggct tgaaaccaaa
     5641 taccaagtat gccgtttatg ttggtgtaga taaccgtagt aatgccaagg caagtatcac
     5701 tgtgaatact ggtgaaaaag aagtgactac ttataccaat aagtctctcg cgctcaacta
     5761 tgttaaggcc tacgcccaca atacacgtcg tgacaatgct acagttgacg atacaagtta
     5821 cttccaaaac atgtacgcct tctttacaac tggatcggac gtctcaaatg ttactctgac
     5881 attgagtcgt gaagctagtg atcaagcaac ttactttgat gaaattcgta cctttgaaaa
     5941 caattcaagc atgtacggag acaagcatga tacaggtaaa ggcaccttca agcaagactt
     6001 tgaaaatgtt gctcagggta tcttcccatt tgtagtgggt ggtgtcgaag gtgttgaaga
     6061 taaccgcact cacttgtctg aaaaacacga tccatataca caacgtggtt ggaatggtaa
     6121 gaaagtcgat gatgttatcg aaggaaattg gtcactcaag acaaatggac tagtgagccg
     6181 tcgtaacttg gtttaccaaa ctattccgca aaacttccgt tttgaagcag gtaagaccta
     6241 ccgtgtaacc tttgaatacg aagcaggttc agacaataca tatgcttttg tagtcggtaa
     6301 gggagaattc cagtcaggtc gtcgtggtac tcaagcaagc aacttggaaa tgcatgaatt
     6361 gccaaatact tggacagatt ctaagaaagc caagaaggca accttcctcg tgacaggtgc
     6421 agaaacaggg gatacttggg taggtatcta ctcaactgga aatgcaagta atactcgtgg
     6481 tgattctggt ggaaatgcca acttccgtgg ttataacgac ttcatgatgg ataatcttca
     6541 aatcgaagaa attaccctaa caggtaagat gttgacagaa aatgctctga agaactactt
     6601 gccaacggtt gccatgacta actacaccaa agagtctatg gatgctttga aagaggcggt
     6661 ctttaacctc agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg cagagattgc
     6721 caagattgaa gccttgaaga atgctttggt tcagaagaaa acggctttgg tagcagaaga
     6781 ctttgcaagt ttggatgcgc cagctcaggc tcaagaaggt cttgcaaatg cctttgatgg
     6841 aaacttatct agtttatggc atacatcatg gggcggagga gatgtaggca agcctgcaac
     6901 catggtcttg aaagaagcaa ctgaaatcac tggacttcgt tatgttccac gtggatcagg
     6961 ttcaaatggt aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg gcaaggagca
     7021 tacctttact gcaactgatt ggccagataa caataagcca aaagacattg attttggtaa
     7081 gacaattaag gctaagaaaa ttgtccttac tggtaccaag acatacggag atggtggaga
     7141 taaataccaa tctgcagcgg aacttatctt tactcgtcca caggtagcag aaacacctct
     7201 tgacttgtca ggctatgaag cagctttggt taaggctcag aaattaacag acaaagacaa
     7261 tcaagaggaa gtagctagcg ttcaggcaag catgaaatat gcgacggata accatctctt
     7321 gacggaaaga atggtggaat actttgcaga ttatctcaac caattaaaag attctgctac
     7381 gaaaccagat gctccaactg tagagaaacc tgagtttaaa cttagatctt tagcttccga
     7441 gcaaggtaag acgccagatt ataagcaaga aatagctaga ccagaaacac ctgaacaaat
     7501 cttgccagca acaggtgaga gtcaatctga cacagccctc atcctagcaa gtgttagtct
     7561 agccctatct gctctctttg tagtaaaaac gaagaaagac tagtatttag taaaacctct
     7621 taacaagatt acggaagcag tctctatctt ttccaatgag gtttatagta cagaaaaagc
     7681 ctgagaagat gtcttctcag gcttttgtta agcacataaa tacaatagtg ctatgacaaa
     7741 atcacccaga aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt
     7801 tgttgatcag gggttgtatt tgattgttgc gtattattgt taggattggt agtcgtacta
     7861 ttatttgtgc ttggagtggt tgagctagac tgtgaagttg aactatctga tgatgagctt
     7921 gaactttcag ttgatggggg ttgttgtgga gcaggtgagc tccacgtaga acgagcacca
     7981 tttttaaata cgaattctcc atttctgtag agcccctctg gtatattcca atcctctgga
     8041 ttgcttcctt cagacaggta ggtcatcata gagcggtaaa ctttggcagc gaccgtaagg
     8101 ccattgccta caagtggtgt cagacggtta gaatagcctg tccatacagc cattgaatat
     8161 ttacgcgtat agccagcaaa tagttcatca ggtgctacaa attgagaggt cttgatgtgg
     8221 ttttcaattt cctcgtctgt atagttagag gttcctgttt taccagcctg agggagccaa
     8281 gcaagatagg catttcgtcc agttccataa ctcaagactg ttttcatcat gtcggtcatc
     8341 atataggctg tcgtttcctt catggcacga gttccgacat tagagaactc tttttcactc
     8401 ccatcactaa agacgacttt atggatatac attggtttat agtaagttcc accatttgca
     8461 aaggcagcgt aagcagcagc catcttttca ctacttgctc catatttttt gtctgattcg
     8521 gttgtgttac ttgaaatggc atttgagtag tgaatacttg ggtagtcgat tccgagacca
     8581 tttaggaaag tcttggcgcg gttgagtccg accttgttta gagtttccac ggctgggacg
     8641 tttcgcgatt gttgcagggc gtattgcaag gtgatgttgc caaagtagcc cctatcccag
     8701 ttataaacag gggtatttgt cccagggtag ttatagggtt catcgtgaac gatagtagca
     8761 gttgaatcgt agacaccgta ctccaaggca ggagcatagt ctgtgatcgg tttcatagtt
     8821 gatccccagt cgcggtttgt ttctactgct tggttaattc cgaaggaaac attgcttgac
     8881 tgatggcgtg ctcctagctg ggcaatgact ttaccgttag aaacatcaac aatggtagaa
     8941 gcgacttgca attcatcgtc tggataggca acgtattcgt ctgtattgta aatatcccac
     9001 agatgttttt gagcttcttg gtctacattt gtgtagacat ccatcccagt tgtgagcagg
     9061 ttatagcctg tttcttcttc aacttgattg atgacttcct tgaggtaatt atccatgtaa
     9121 gcagggtaat tacttgctga tttgagactt tgtagtccat cagtaattgg tgtattgact
     9181 gctttctcat actgttcagc agagatgtag ccttgatttt tcatttcaga taagaccaag
     9241 tttcggcggt cttgggctgc ttctggatgt gaataggggt catattggtt tggtgcctga
     9301 ggcattccag ccagcaaggc taactgaggt aaacttaaat tattgaggtc tttaccatag
     9361 tagttttgag ctgctgtctg cattccatag ttcccattag acatgtagac cttatttata
     9421 tagtaggtca agatttcttg cttggttgct ttttgttcta actgaatcgc taaccaagct
     9481 tcctgagcct tacgagaaat agtctggtcg gaagtcgaag ttgaaaagta agtcaactta
     9541 atcaactgtt gagtgagagt tgatccacct tggagggaat tgctttgcag attgcgcaag
     9601 aaagctccca ggatacggat ggtatcaatc cccctgtggt cgaagaagcg atggtcttcg
     9661 atagaaacga ttgctttaac caaatctgtg ggaatatcat tagcttgggc attgacgcgg
     9721 cgttcagaac ccaagtcagc aatgagttga tttttattgt cgtagatttt actagaagtt
     9781 gttgcaacta gtttactctc ggataggcta ggagccttgc taacgtagta gaaaaaaact
     9841 cctccgccta agacaatggc tgcgataacc aagcttaaga agctaatgct cagatacttg
     9901 attaggcgca gaatcgttgg tttgttcatc ttgttttacc acctaataaa tgttctttga
     9961 taacattgag ataaggaatt tgagggaagg caccagcctt gatttcatat ccatattctc
    10021 gaatatattc aagtggcatt gatttttgtc ccttatcttg atgatagaag cgaatcaaat
    10081 cgaatgccgg caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga
    10141 ttccttgttg ggcaaggact tgttccatat gctgaatctg atgtggatga aaatttttca
    10201 tcggaatcgc acgtttttgt tttgtttcct tgacttcaaa gtcgatgtaa tatccattat
    10261 aaacgccaga atagtccgtc gttgaagctt gtcgaaaata ggcttcaaca atcttggcac
    10321 gacttcgttg tggatagtcc acttgtacga tttgaatagg agttggtttc ttatgtataa
    10381 cagccaagcc ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca
    10441 ttcctcgatt tgcgaaattt ttgggttgag aaagagatgt ttgtcttttt tgtgatgaaa
    10501 ctttatgtgg atagttgacc ataattctcc ttattggtac aataacatca ctttattata
    10561 ccataatttg cacagaaagg gttaaaaatg gctacagctt tggttttagg ctattctgct
    10621 tttgatttgg gtttattttc tgataaggat cctcgtctta aattgattaa aaaagcgata
    10681 cgtaaagatt tagaggctat ggcagcagat ggggtgtctt ggcttgtgtt tacagggagt
    10741 ttgggttttg aatattgggt tttagaggtt gctcaggaga tgaaaaccga atacggtttc
    10801 cagttggcca ccatttttgc ttttgaaacc catggggaaa attggaatga aggcaatcag
    10861 atgaaactga gtcgctttaa gcaggtagac tttgtcaaat atgcttatcc tcgctatgaa
    10921 cacaaggggc agttaagaga ttaccagcag tttttgctgg aaaacacgac tagttcctat
    10981 cttttttatg atgaagaaaa tgaaacaaaa ctagcttatt tttaccaaaa gatgaaaaat
//
LOCUS       DAVMHG010000060        10893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMHG010000060 DAVMHG010000000
VERSION     DAVMHG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.060"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(99..449)
                     /locus_tag="VYR08_001229"
     CDS             complement(99..449)
                     /locus_tag="VYR08_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466756.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(589..924)
                     /locus_tag="VYR08_001230"
     CDS             complement(589..924)
                     /locus_tag="VYR08_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466757.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1012..2322)
                     /gene="der"
                     /locus_tag="VYR08_001231"
     CDS             complement(1012..2322)
                     /gene="der"
                     /locus_tag="VYR08_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV3466758.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2336..3049)
                     /locus_tag="VYR08_001232"
     CDS             complement(2336..3049)
                     /locus_tag="VYR08_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV3466759.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3046..3942)
                     /gene="dnaI"
                     /locus_tag="VYR08_001233"
     CDS             complement(3046..3942)
                     /gene="dnaI"
                     /locus_tag="VYR08_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV3466760.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3943..5112)
                     /locus_tag="VYR08_001234"
     CDS             complement(3943..5112)
                     /locus_tag="VYR08_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV3466761.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5113..5586)
                     /gene="nrdR"
                     /locus_tag="VYR08_001235"
     CDS             complement(5113..5586)
                     /gene="nrdR"
                     /locus_tag="VYR08_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV3466762.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5726..6091
                     /locus_tag="VYR08_001236"
     CDS             5726..6091
                     /locus_tag="VYR08_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3466763.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6096..6791
                     /locus_tag="VYR08_001237"
     CDS             6096..6791
                     /locus_tag="VYR08_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466764.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6808..7575
                     /locus_tag="VYR08_001238"
     CDS             6808..7575
                     /locus_tag="VYR08_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466765.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7928..9127)
                     /locus_tag="VYR08_001239"
     CDS             complement(7928..9127)
                     /locus_tag="VYR08_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466766.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFLLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9124..10017)
                     /locus_tag="VYR08_001240"
     CDS             complement(9124..10017)
                     /locus_tag="VYR08_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466767.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10133..10213
                     /locus_tag="VYR08_001241"
                     /pseudo
     CDS             10133..10213
                     /locus_tag="VYR08_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10723..10893
                     /locus_tag="VYR08_001242"
                     /pseudo
     CDS             10723..10893
                     /locus_tag="VYR08_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3218 a         2384 c         1973 g         3318 t
ORIGIN      
        1 tccactatac aagatgaata tttctctcaa gtttcataat caaaataaaa atgactgtat
       61 ctatagctcc ataaacaaat catagataca gtcatttttt agttcctaca ccttccttta
      121 tgcacacctt tagatttccc tagttcccgc ctcaagcgaa tcattttttt gaaaaagaca
      181 taggctaacc aatcactagc aaaagcgata agtagtaata atagccaaat accccataac
      241 ttgatatctg tcaaatttct caagacgata ttgaaaaaca gaactgaaaa aactgtccaa
      301 gcaaggctaa aaagagcata gagggtgatg taaatccagt aaaaatagta aaaaattggg
      361 aaaaatttac tttctctatt atccttgtca atccagctga aaaagtaaaa acagggtaat
      421 aagattatca ttttaaacaa gtgttccatc acaagcctcc ttttttaaat tgttttcttt
      481 agtttcgttc tattaaatgt tattaccaat ataaaccttg gaatagcgtg taataaatga
      541 ttttagaggt accataagcc acctcctaca aatagaaacc gatataaatc aatgccttcc
      601 acccttagac ttccctagtt cctgtctcaa gcgaaacatt tctttgaaac aggaataagt
      661 taaccaattc ataccaatag ctagcagaat aaaaagaaac caaatgcccc ataacttgat
      721 atctgtcaca tttctcaaga cggtattgaa aaacagaact gaaacaactg tccaagcaag
      781 gctaaaaaga gaatagaagg ggatgtaaaa ccagtaaaaa taataaaaaa ttggaaaaaa
      841 cttactattt ctgttggcct tttcaatcca gttatcaaaa taaaagtacg gtgctaaaag
      901 taagaattta aacaaatgtt ccatcaccga catccccctt cttttgatag cgttttctat
      961 tattttatta tatcaaaaaa atccggaact gtcattccag attctacttt tttatttgcg
     1021 ttttcttgcg atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc ggatttgatt
     1081 ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct tcattgacaa agatgacaaa
     1141 ggttggtggt ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt ttcctttgtc
     1201 tgtcggtgtt gggttgatgg caatggcatc catgatgaca tcgttcaaga cagctgatgg
     1261 aatacgtgta ttttgacttt cgctgatttg cttaatcatc tcaggaagtt tgtggagacg
     1321 ttgcttggtt aaagctgata caaagataat cggtgcgtaa ggcaggtatt ggaactgctc
     1381 acggatatct tcttcccagt ttttcatagt gtggttatct ttttcaagcg tatcccactt
     1441 gttgaccacg ataatcatcc ctttaccagc ttcatgagca aatccagcga tacgcttgtc
     1501 gtattcacga atcccctctt ccgcattgat gaccattaag accacatctg aacggtcaat
     1561 agcacgcatg gcacgcatga cagagtactt ctcagtattt tcataaacct taccagactt
     1621 acgcatacca gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg taaagtgggt
     1681 atcaatagcg tcacgagttg ttccagcaac aggactggca atgacacggt cttctcccaa
     1741 gatagcatta atcaagcttg attttccaac gttaggacga ccaatcaagc taaacttaat
     1801 gacatctgga ttttcttctt catattcatt tggaaggttt tccacaatcg catctagtac
     1861 atcccctgta ccgataccat ggacagatga gataggcagt ggttcaccca aaccgagagc
     1921 atagaaatca tagatatcat ttctcatctc agggttgtcc accttgttga ctgcgaggat
     1981 aactggtttg tgggtcttat aaagcttacg agctacgtat tcgtctgcat cagtaattcc
     2041 ttccttacca gacacgacaa aaacgataac atctgcttct tccatggcaa tttctgcctg
     2101 gtgcttgatt tgttccatga aaggagcatc gacatcatca attcctcctg tatcaatcat
     2161 gctaaaagaa cgattgagcc actcacccgt tgcataaata cggtcacgtg tcactccttc
     2221 gacatcttct acaatggaga ttcgctcacc agcgatccga ttaaataggg ttgatttccc
     2281 aacattggga cgtcctacaa tggcaatagt tggtagggcc ataatttctc actttctaca
     2341 ataatttctt ctgttcaaga ttttttctag ttgagcttgg ttcagcttga ccaaactgtt
     2401 ctgctaggcg ctgactccag cttgtggtcg cacgcgcccc agcatagtca gcctgaacac
     2461 ggtcataagc ttggattgcc tcagttgact gttcttggta ttcttcctca aagacaacat
     2521 tctctagtgg cagtctcggt ttcatatcat gatgttgatt tggcacaccc agtgccatcc
     2581 caaagacaga ataggtgtag tcaggtaggt taaagagctc tgccacttct tcagacttgt
     2641 atcgaaccaa accgataatc acaccaccat agcccaagct ttcagctgcc aacaaggcgt
     2701 tttgtccagc aagagctgca tcgaccgaac taatcaagag accttccaca ccttggggtt
     2761 ggaaggtgtc ggtatgaagt ctggctccct tttctgctcg gttcaaatct ccgacaaaga
     2821 gaaggaaaac agcagactgg cgaatggctt cttgaggtac caattcatac aaggcatctt
     2881 tcttctcttg acttcgtacc acaatcacag agtaggattg gaaattcttc caagatgatg
     2941 ccatctgggc tgctgtcaaa atctcattta agtctacttg gggaatttct tgctctttaa
     3001 acctgcgcac tgaagtatga gccttcatca atttaatggt ttctgtcatc gacggtttac
     3061 tccttctaaa cgagtctcct cagccaaata acggatgcgt tccatgaccc gtctggcttc
     3121 ccaggtttcg tcatttccat gtttcacttt cgcaaaatgc ttctccaaat cttcaaagtt
     3181 gaagttggat gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga gaatgacctg
     3241 caggatttca tcacgcaccc aagcggtgga ttgctcggcg ccaatatcat ctaaaatcag
     3301 gacctcagac agcttaatct catccaccaa ggtcttaaca ttgccatcac tgatagcatt
     3361 tttgacatca atgacaaagc taggatagtg gaggagagtt gatgaaacac cacgtttttc
     3421 tgataaatca tgagctaagg ccgccaccat gaaactttta cccacaccaa agtctccata
     3481 taagtaaaga ccttttcgaa tagctggata ttgctccacg aaggctagta gcttttcaaa
     3541 aactggtaag cgccccaaat catccaagtc aacttgagcc aaactagctt tcttgagact
     3601 ggctggtaga ttgattaact tgagacggtt cttaatagcc gcttcttttt cagccgcgat
     3661 tagctcagga gtttcttcat atgaaacatc tgcatagcca tgattcttaa ccaaaatcgg
     3721 cttgtagcct ttggcaatat aatccgtatc cccacggaga aacttgtcac gctcggtgat
     3781 gtactgatta aacttggaga tactgcgatt taattcctct ggagtcaagg attcttgctg
     3841 gataaaagcc gcaacatcag ggtccttcat gattttctgg accaaatctt gataataaaa
     3901 acggctgggt tgacgtttga gtacgtctcc gacactttcc atctaatctc ctcctttttc
     3961 taatcgagct aatagttctt gcttcttacg ttctagttcc agacgagttt cctcgctggt
     4021 ttcattctta tattcaggat tactccattt aggaacattg gtttttactg gggcagtctg
     4081 attctgtttt tgtgtttttg ctttctgtcc tcgctcacgg atgcgcaaga ctgccttttc
     4141 tgccgaatga atcttttgat aggcatagtc attggctacc ttcatggcat atttctcatt
     4201 gatatttgcc gaatcgacct tattaaaggt caataaaaga atgatattga tgacttcgtc
     4261 cagcaagccc aagccagcca tctgttgcaa gagttctctt tctgtttggg taatggttcc
     4321 cttgcgtgtt tgcttgattt ctgccaagaa ctgcagggca gttttacttt tggcttcttt
     4381 gataatggtt gtttccttaa gactaaagtc agaggaaact ggtttttgag caattttttc
     4441 acgcatgcgt ttggttgaaa taacctgaga aacagctgtt gacttggcca attgataggt
     4501 ttcaaaccaa gtccatttct tctcctcggc aatagcaaag aggtttaaga catcggactg
     4561 ctcatccgca aaacgaagtc catctcgagc catcagctgg cgaaaatgtt ccaagtcaaa
     4621 atcattggcc actttcttct tgagaccaag gtcttcttga ctgcctagtt ctgccaattc
     4681 tggaaagact tgattgagtg agacaggtat ttcttcacca tcagcacttt caactttcaa
     4741 atcctccaca gcagagtcgc caatcttttt ctctaagagt ctgcgataaa caggatgctc
     4801 caagaagtct tgactagata gaggagcatg gagggctagc tgataaacat cccccttttg
     4861 atagagggtc aagagattaa aagcagataa gattttcaat gattttatca gtctatccat
     4921 cccaaagttg agatgattga ggatacttga aaaaagatat tcctttctac cattatccca
     4981 aaaactgatt gtataaagat aaaggctcag tgcctcctga ccgataatcg ggaggtagca
     5041 ctgtaccaga gatgaggtat cttgcgacac ccgattattc tttagataag aaaaacggtc
     5101 aattggcttc atttatcttt cctttttctt tttagaggac tgggtgattt gttggagcaa
     5161 gctctctaac tcactgacat ccttaaaact acgatagaca ctagcaaaac gtacataggt
     5221 aatctcgtcc aattcagcca actcctccat gacgagtgaa ccaatgtcct cactttgaat
     5281 ttcattttca tttcgaccac ggagtttctg ttcgatacga ttgactacca tgttgatttc
     5341 atcacttgac acaggacgtt tctgggctga gcggataatc ccattaaaga ttttatctct
     5401 ggagaattgt tcccgtgtac catctttttt aacaaccact aaggttcttt cttctactcg
     5461 ttcgtaggtt gtaaaacggt gttggcattc gtcgcactca cgtcttctac gaatggtgtt
     5521 cccttcttct gcttggcgac tatcgataac acttgacttg gtagccccac attttggaca
     5581 acgcatcctt tccctcctta tcgttttctt ttcattatac cattttttaa gcgattccca
     5641 aaacaattct tctttttgct tgacaagttt tttgttttgt tgtattattt aattaagaca
     5701 aaaggataag agaaaggaga ctaagatgtc ctggacattt gacaacaaaa aacccatcta
     5761 tttacagatt atggagaaaa tcaagcttca gattgtttcc catacactgg aacccaatca
     5821 acaacttcca accgtgaggg agctagctag cgaggctggt gtcaatccca ataccatcca
     5881 aagagcctta tcagaccttg aacgagaagg atttgtctac agcaagcgaa caactggacg
     5941 atttgtgact aaggataagg agctaatcgc ccagtcacgc aaacaattat cagaagaaga
     6001 attggaacac ttcgtttcct ccatgaccca ttttggctat gaaaaagaag aactaccagg
     6061 cgtagtcagt gattatatta aaggagttta agcctatgtc attactagta tttgaaaatg
     6121 tatccaaatc atatggagca acaccagccc ttgaaaatgt ttctcttgac attccagctg
     6181 gaaaaattgt cggccttctt gggccaaacg gctcaggaaa aacaaccctg attaaactaa
     6241 ttaatggcct cttacaacca gatcaaggac gtgtcctcat caacgacatg gacccaagcc
     6301 cagcaaccaa ggccgttgta gcttatttgc ctgatacgac ctatctcaat gagcaaatga
     6361 aggtcaaaga agccctaacc tacttcaaga ccttctataa agatttcaat cttgaacgcg
     6421 cccatcatct acttgcagac ctgggcattg atgaaaatag tcgtctcaag aaactatcaa
     6481 aaggaaacaa agaaaaggtt caactgattt tggttatgag ccgtgatgct cgtctctatg
     6541 ttttggacga acccattggt ggggtggatc cagcagcccg tgcttatatc ctcaatacca
     6601 ttatcaacaa ctactcacca acttctaccg ttttgatttc tacccacttg atttctgata
     6661 tcgagccaat cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc cgtcaaggaa
     6721 atgtagatga tattcgctac gagtcaggtg aatccattga ccaactcttc cgtcaggaat
     6781 ttaaggccta agcaaaggag attatttatg ttttggaatt tagttcgcta cgaatttaaa
     6841 aatgttaaca agtggtattt agccctctac gcagccgtgc tagtcctttc tgccctcatc
     6901 ggaatacaga cacaaggctt taaaaatcta ccttaccaag aaagtcaggc tactatgcta
     6961 ctttttctag ctacagtctt tggtggcttg atgcttacac ttgggatttc aaccattttc
     7021 ttgattatta aacgcttcaa aggtagtgtc tacgaccgac aaggctatct gactttgacc
     7081 ttgccagttt ctgaacacca tatcatcaca gccaaactaa tcggtgcctt tatctggtca
     7141 ttgattagca ccgctgtatt ggctctaagt gctgttatta ttctggcttt aacagctcca
     7201 gaatggattc ctctttctta tgtgattaca tttgtagaaa cacatctccc tcagatcttt
     7261 cttacaggta tatccttcct actaaatact atttcaggaa tcctctgcat ctacctggct
     7321 atttccattg gacagctttt caatgaatac cgtacagcac tcgctgttgc agtctacatt
     7381 ggtatccaaa tcgtcattgg atttattgaa cttttcttca atcttagttc taatttctat
     7441 gtcaattcac tggtaggact caatgaccat ttctatatgg gagcaggtat agccattgtt
     7501 gaagaactca tattcatagc tatcttttat ctcggaacct actacatctt gagaaataag
     7561 gttaatttgc tttaaataat ttttacctag atatgtaaca tactcataga acaaaagaga
     7621 ccaggcaaaa agtctttaca attagaaaac gcatagtatc aagtgttaaa aatccttgat
     7681 attatgcgtt ttttgtgagc tgacttattt cctttcacta tatcgcaaaa tgaaataaga
     7741 acggaacgat gggattttgg aattcaaatc aatttataag aatgttttag aagtaatatt
     7801 atcctattcc agattcagtt cactatacaa ttgagttttc aagcaacctg tttacataat
     7861 gtgtacataa ttaggttcgt gattccaccc ttttcacctt taaaaacctc gctttcgcaa
     7921 ggctcttcta tttataagat aaggcacgtt taaaggtttt ccaaatccct aaatcatccg
     7981 tttgaagaac gagactagca tacatgcgtc cgataaatcc tgttgctacc accgcaaaaa
     8041 tcactgtaat agcaagtgaa atccatgctt ccgctccctc cgcatagcca ttaatcgttc
     8101 gaaacggcat aaagaaggtc gaaataaagg gaatataaga accaatcttc aagaggagat
     8161 tgtcaccagc tgcacctaga gctgtcactc caaaaaaacc acccataatc aaaatcatca
     8221 aaggcgacaa ggctttccct gagtcttcag gacgagaaac catagatcct aggaaggctg
     8281 ccaagactac gtacatgaaa agactgatca aaataaagag caaggtattc agtgagatag
     8341 catctcccaa gtgatccaaa ataccagact gagccaagaa tggcaaatct ttaaagagca
     8401 aaacggcagc cagaccacct acaacataga tcccaatatg cgttaaaatc actagaaaca
     8461 gagccatcat ccgcgcatag aaatagtgac ttgcccttat gctagaaaag acgacttcca
     8521 taattttggt gcctttttca ctggcaactt cctgagctgt tacacccgca taggtaatca
     8581 gaatcatata aagaaggaat cctaaggcac ctgctgcaat tgtttgaata aactttttat
     8641 tttccttggc ttcatcaatc ttttctgtga attgaattgt ctgcgctaag cgtttttcct
     8701 gctcttgaga caaggaagca gttgaacgat taagctgatt ttgcagttca ttgagtgtac
     8761 ctgtaacctc aaatttaatt ccattttcaa gcgatgtttc gccatgataa actgccttta
     8821 gaacactatc ttcttgatca atggtcaaat aaccttttaa tttttcttct ttaattgctt
     8881 ctttggcact tgcttcgtct ttatagtcga agttaacacc atttacattc ttcagtcctt
     8941 ctgctacaga tggcactgtt gtcactactg ccactttatt atttttagcc atagaagaac
     9001 cttggagatg cccaattcct acagagattc ctaaaaagag gaacggcgaa atcaccataa
     9061 agaagaaact ccatgactcg acatgtcgaa gataggtttc cttgattaca acccacatat
     9121 ttctcatact tccactcctg attctagttt aaagatttca tcgatagttg gcgcttgttg
     9181 gtcaaatgtt gcgatatatt gaccttgagt caagattggg aagagttccc ttccagcgct
     9241 ctcatcctcc aaaatcaatt tccaactgcc ttgtttggtc aagctcacct gtttgacatg
     9301 aggaagattt tccaattctt ccttgcttcg ttcacttgaa acaaagagac gcgttttccc
     9361 gtattgattg cggacatcct gaactggtcc gtgcaagacc acacggccat ctcgaatcat
     9421 cagaatatcg tcacaaagtt cctcaacatt ggtcatgaca tggtcagaaa agataatggt
     9481 tgctccgcgc tctttttcct gaaaaatgac ttgtttgagc aattctgtat taactgggtc
     9541 caatccacta aaaggctcat ccaagataat caggtctggt tcatgaatca gagtaataat
     9601 gagctgaatc ttctgctgat ttccttttga cagactcttg attttatctg tcagctttcc
     9661 tttcacttcc aacctcttca tccattgagg gagtttttct ttgacttctt tggcatccat
     9721 gccttttaga gtcgccaagt agcgaacttg ttcaagaact gtcaatttag gcatgagact
     9781 gcgttcttca ggcagataac caatccgagc ataggtctcc tgacgaatat cctgaccatc
     9841 cagaccgatt tctccctgat attctaggaa tttcaaaata ctatggaaaa tcgttgtttt
     9901 tccagcacca ttttttccga ctagtcccaa aatacgacct ggtcgcgctt gaaagtcaat
     9961 accaaacaaa acttgcttgg agccaaaact tttctctaga cttcttactt ctagcatctt
    10021 tcacctccga aatttcttgc actcattata ctcctttttg atagccttta caatgttttt
    10081 gtccattttt agaagactat tgctgtgtaa aatatagcct ggagcacttt tatactcaat
    10141 gaaaagcaaa gagcaaacta ggaagctagc cgtaggctgc tcaaagtaca gctttgaggt
    10201 tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga
    10261 cgaagtcagt aactatatct acagcaaggc gaagctgacg tggtttgaag agattttcga
    10321 agagtattag tgataaatcc attatacagt agcaaactta atttatacct tccgctcctc
    10381 aactgtctat ttttaatcct gaattgttat ttgagtaact cctttttcct cgtaaagttt
    10441 tcttcctcta aaacttctgg aaaaaggcta atagtttcag acaacatttt tataagaaac
    10501 aagttcatct gtcatttcaa gaaggagtaa tcctttatct actaatggac ggaacagaat
    10561 tcaaccgctt gtccgatatg ttttctaagg attatatagt aaaatgaaat aagaacagga
    10621 caaattgatc aggacagtca aattgatttc taacaatgtt ttagaagtag atgtatacta
    10681 ttctagtttc aatctgctat atctattatg cacatcacta taggatctaa tgaaaatcac
    10741 aacaggctca ttcatagatg gttacctcag ggaactaaga aaacaactac aaaggaagtc
    10801 gcatttatcg aaaagtggat taacaactat cctaaaaaat gcttgaacta caagtccccc
    10861 agagaagact tctggatgac taacttgaac ttg
//
LOCUS       DAVMHG010000061        10778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMHG010000061 DAVMHG010000000
VERSION     DAVMHG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.061"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..156)
                     /locus_tag="VYR08_001243"
     CDS             complement(<1..156)
                     /locus_tag="VYR08_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3466768.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKK"
     gene            complement(295..819)
                     /locus_tag="VYR08_001244"
     CDS             complement(295..819)
                     /locus_tag="VYR08_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV3466769.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(872..1108)
                     /locus_tag="VYR08_001245"
     CDS             complement(872..1108)
                     /locus_tag="VYR08_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV3466770.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(1176..2096)
                     /locus_tag="VYR08_001246"
     CDS             complement(1176..2096)
                     /locus_tag="VYR08_001246"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV3466771.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2286..2689
                     /locus_tag="VYR08_001247"
                     /pseudo
     CDS             2286..2689
                     /locus_tag="VYR08_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2905..3486
                     /locus_tag="VYR08_001248"
     CDS             2905..3486
                     /locus_tag="VYR08_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3466772.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3483..4760
                     /locus_tag="VYR08_001249"
     CDS             3483..4760
                     /locus_tag="VYR08_001249"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV3466773.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4778..5440
                     /gene="deoC"
                     /locus_tag="VYR08_001250"
     CDS             4778..5440
                     /gene="deoC"
                     /locus_tag="VYR08_001250"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV3466774.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5427..5816
                     /locus_tag="VYR08_001251"
     CDS             5427..5816
                     /locus_tag="VYR08_001251"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV3466775.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5906..6958
                     /locus_tag="VYR08_001252"
     CDS             5906..6958
                     /locus_tag="VYR08_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV3466776.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7102..8637
                     /locus_tag="VYR08_001253"
     CDS             7102..8637
                     /locus_tag="VYR08_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466777.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8630..9688
                     /locus_tag="VYR08_001254"
     CDS             8630..9688
                     /locus_tag="VYR08_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466778.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9691..10647
                     /locus_tag="VYR08_001255"
     CDS             9691..10647
                     /locus_tag="VYR08_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466779.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3072 a         1911 c         2449 g         3346 t
ORIGIN      
        1 tttcttcatt agacttccac aatcagggca agatggagcc tcataatcca gcttagcgat
       61 aatttctttg tgggtatcca tattgatgat atctagaatc ttgatgtttg ggtctttaat
      121 atcgagcagt tttgtgataa aatgtaattg ttccatatga atctttctaa tgatggtttt
      181 gtcgcttttc attataggtc atatgggact ttttttctac aataaaatag gctccataat
      241 atctatagtg gatttaccca ctacaaatat tatagagccc aaaaaggaag ccctttatga
      301 attgtaggac ttccttttct tatccagaaa ttgatctagc tctctctgat ttcgaagaat
      361 agtgacttta tgtgaatatt cttggcaaag tttttggtaa ttttcttttt gagttttgct
      421 acgcccatcc caaagaatcc atctgataaa ctcccactca aagcgttcag ggcaatctac
      481 cgccatactt tctctgactt ttccacggta tttaagataa cgcttaaagg ctctaaagag
      541 acaggtcaat ggcgaaaaat tgagaaagat gatttggtca gcttcttgca ttcgttcttg
      601 gtagtagcac caagaataat taccatcgat gacccaagct ttatgcttgg tgagaaagtt
      661 ttttatctcg gttaacatcc attcgcagtc actgtcttgc caaccaggtt gaaattggag
      721 tgtgtccatg tgcagttttg gaatggagta gtagttagat aacttttctg ctatagttga
      781 cttaccagaa ccagaatatc cgataattgc gattttcatt ttctaccttt tcctatttgg
      841 agacaaaaaa acagcctcta tggactgttt cttatttaac aagtttagct gaaagacgag
      901 ctttatcgcg gcttgctttg tttttgtgaa tcaaaccttt agtttctgct ttatcgatag
      961 ctgagctagc agcacggaaa agttcttcag atgggtttgc ttcgaaagct tttatagcag
     1021 tacgcatagc tgatttttga gctgagttct tttcgttttg tttaacgttc aattcagcgc
     1081 gtttgatagc tgatttaatg tttgccaatg ttcttacctc catatttact aactatacca
     1141 ttatatatga aaacttacgt tttgacaagg gaaaattact tttttaagta aatttcatcg
     1201 atttcatggt tcttgctttt atgaagaatc acttctgcac gatttctggt tggttcaata
     1261 taattttgca gatttgtgag attgatactg atccagacct gatgggcaaa ggactccact
     1321 tccccaatcg gcatctgagt aaaacgataa tagtagctat cagggtcgtt ttgggctaga
     1381 ctcagcattt tcaagaaacg gtccagatac caactttcaa tatcatccac tccagcatct
     1441 acatagatgg aaaagtcaaa gaagtcagtg atatagagac gatcgttttg tggattttga
     1501 aagacattga ttccctcaac aattacaaaa tcagcagctt tgacactttg tttctcttcg
     1561 ggtacgatgt cgtaaacttc atgagaatag acaggaatat ctacatcttg tccatttttg
     1621 atgcggtcca agaagttgag aagagcttcc atatcatagc tttcaggaaa tcctttacga
     1681 tttaaaatcc cctgctcaat caaggtttga ttgggataga gaaaaccatc agttgtaacc
     1741 aactcaaccg tagcatctgt aaacgtacgg gacagtagga tttgaagtag gcgactggtt
     1801 gtggattttc caacggcaac actcccagaa accccaataa taaaaggttg agatttactt
     1861 tcacgttgga ggaaaattcc ttttgaaaag gctaaatctt ccttagttcg cttgtaaatc
     1921 tgaatcaaat gagccaaggg gagatagata tctgtaacgt cttggagact gatttggtca
     1981 ttaaaactct tgatagattc caattcttct tctgtcaaag gaggtgttgt ctttcgatgt
     2041 aaagattgcc aagtctggcg gctgattttt tcaaaatgta aaaattcgtt ggtcatttgt
     2101 tttcccctag atattttgtc tatcatacca taaaaagcta aaaaaataaa gcggtttctt
     2161 gaatgtggta agtcaatggt atatagtata agtgatcaat ttattgatga aataggagat
     2221 agattctact gatttataag cttataaagg gggcttttta gttcacaact tataggaggt
     2281 gtactatgaa aatcttaaaa cgttacatat tggaactctg ttttatttta agttttgctt
     2341 taccttttat aaaaggaacc aatgcagata atggtagatg ctttgtggaa acctattacg
     2401 gttttacttt tttgatggaa catgctattg taacagctgt ctttatttgt tcgttcttaa
     2461 ttgctttctt actaaaaaac gatggacgaa atggattgct gcgggtagtt attgcttttt
     2521 agtcctacgg attgcgacag agggttattt cttccacatg tcactagaag atttgatacg
     2581 actttggaca agtttggaaa tcctgacaca aacgtatcag ttgggctttt atctaaacat
     2641 tttgttagga attttgttga taataaagta ttttaaggtt aagcaataga aataataatc
     2701 tgcctgatta tagatgataa actttttgtt tttatctgga ctgtgatatt tatagttcta
     2761 aatggtttat tatttataag tgttgaaaga gattgaagaa aatggcttcg tgacttgctt
     2821 tcttcggtct ttttcgtttt attatcaatt tgtaaacgat ttacaatttg agtcaaatta
     2881 tggtaaaata gtgtcatgag taaaatgtac tatgcagaaa atcctgacgc tgctcacgac
     2941 attcatgagt tgagagtgga cttgttggga gaaaaaatga cttttttgac agatgcgggt
     3001 gtttttagca agaaaatggt tgactttgga agtcaactct tgctcaagtg tctggatgtc
     3061 aaccaaggag agacagtcct agatgtaggc tgtggttatg gtccattagg tttgtccttg
     3121 gtcaaggttt acggagttca ggcgaccatg gttgatatta acactcgtgc cctggattta
     3181 gctcagagaa atgctgaaaa aaataatgca aaagcgacga tatttcaatc taatatttat
     3241 gcaagagttc aagggcgatt tgaccatgtc atttccaatc ctcctatccg tgcgggcaag
     3301 caagtcgttc atgaaatcat cgagaaaagt aaagatttct tggaaattgg tggagatttg
     3361 accatcgtca tccagaaaaa acaaggagct ccaagtgcca agtctaagat ggaagatgtg
     3421 tttggcaatt gtgaaatcct taaaaaagac aagggatact atatccttag aagtgtgaaa
     3481 gaatgagagc agttgattta atccaaaaaa aacgagacgg tcaagaactg acttcaagtg
     3541 aaattgaatg gctgatagaa ggctatgtat caggaactgt tcctgattat cagatgtctg
     3601 cctttgctat ggctgtttat tttaaaggaa tgacgactcg agaaatctct gatttaacga
     3661 tgaatatggt taagactggg caagagtttg acctgtcagc cattgatggt gtgaaagttg
     3721 acaagcattc tactggtggt gtcggcgata aggtaacctt gattttagct cctcttgttg
     3781 ctagcttcgg tgtgcctgta gcaaaaatga gtggtcgtgg tcttggccat acaggcggaa
     3841 caattgataa attggagtcc attaagggct atcaagtaga acgtagtcaa gaggatttta
     3901 ttcgtcaggt tcaggatatt ggagtatctg tcattgggca atcagaccag ctggtcaaag
     3961 cagataagct tctctatgcc cttcgtgatg tgaccgcaac tgtcgacacg attcctttga
     4021 ttgcgagttc ggtgatgagc aagaaaattg cggcaggggc ggatgctatt ttgctagacg
     4081 tgactgtcgg tgagggtgcc ttcatgaaga cggttgatga ggcgcgtgag ttggctcaaa
     4141 ctatggtaga tcttggtaag gttgttggac ggaagacagt agcagtgatt acggatatga
     4201 gccagccttt gggacgagcg attggaaatc gtctggaaat ccttgaagca ttggagattt
     4261 tgcaaggtca aggacgtcag gatattaccc actttatttg tgaattggct caaattatgc
     4321 ttggtttggc aaatgttaac aaaactgttg aagaagttca ccaacatctt gagaatggtc
     4381 aagcactggc taagtttgag gaaatggtgc aagcccaagg tggagacttg gaagacctct
     4441 atcgtcctgt aaatgtagct catgtggtgg aaatccctgc tcaggaaacg ggtgtcattt
     4501 cagctcttcc agctatggac tttggtcttt atgccatgag gctgggtgct ggtcgtgcag
     4561 ttaagtctga tgccttggac tatgaaacag gaattgtttt tgaaaagaag attggagact
     4621 ccgttcaaaa gggtgaaatt gttgcaaaag tatacacaaa tgaaaaaatt ccacctcaac
     4681 tagttacaga tttccaaaaa tgtgttaaaa taagtgatga agtgaaaaaa atccgtgaaa
     4741 ttgtagaaat catctcctaa atggagggaa aaccgaaatg aaattaaata aatatataga
     4801 tcatacgctt ttaaaacaag atgcaaagaa aaaacaaatt gatagtttgt tgtctgaggc
     4861 tagagagtat gactttgcca gtgtttgcgt taatccgacc tgggttgaac atgctaaaaa
     4921 aggacttgaa ggcacagatg ttaaggtttg cacagtagta ggtttccctt tgggagcaac
     4981 aacttcagcc gtgaaagcat ttgagacaaa agaagctatc caaaatggtg cagatgagat
     5041 tgatatggtg atcaatgttg gagctctcaa atcaggtaat ttagccttgg ttgagtcaga
     5101 tattcgcgca gtagtggaag caagtggtga taagttagtg aaagtcatta ttgaagcttg
     5161 ccttctgaca gaccaagaaa aagttgttgt ttgccaattg gcccaaaaag ctggggctga
     5221 ctttgttaaa acatctactg gtttttcaac tggtggtgct acgatagcag atgttacatt
     5281 aatgcgtgaa acagttggat ctgatatggg agtcaaggct gctggtggag ctcgttctta
     5341 tgcagatgct cttgcctttg tcgaagcagg tgcgacccgt atcggaacgt cagctggggt
     5401 agctatttta aaaggagaat tggcagatgg cgactactga gttgattgaa ctggcaattg
     5461 aaaccagcaa acatgcatat gtcccctatt ctcactttcc tatcggagca gttttagtag
     5521 ccaaagacgg aagtgtttac acgggagtga atatcgaaaa tgctagctat cctttgacca
     5581 attgcggtga gagaacagct atttttaagg ctatatctga aggccaaaga gaattttcag
     5641 aattgattgt ctatggtcaa actgaaaaac cgatctcgcc atgtggtgct tgtcgccaag
     5701 tgatggctga attttttgaa caagatctaa aagtgacctt agtcgcaaaa gataaatcga
     5761 cggtcgagat gacggtcggg gaattacttc catactcttt tacagactta aactagtctg
     5821 agtcgctctc tgagtggcac ggtccttgtg gccaaccaat ccatacttgc aacatcgttg
     5881 cacatcttat ttaggaggtt cagtaatgaa caagaaacaa tggctaggtc ttggcctagt
     5941 tgcagtggca gcagttggac ttgctgcatg tggtaaccgc tcttctcgta acgcagcttc
     6001 atcttctgat gtgaagacaa aagcagcaat cgtcactgat actggtggtg ttgatgacaa
     6061 atcattcaac caatcagctt gggaaggttt gcaggcttgg ggtaaagaac acaatctttc
     6121 aaaagataac ggtttcactt acttccaatc aacaagtgaa gctgactacg ctaacaactt
     6181 gcaacaagcg gctggaagtt acaacctaat cttcggtgtt ggttttgccc ttcataatgc
     6241 agttaaagat gcagcaaaag aacacactga cttgaactat gtcttgattg atgatgtgat
     6301 taaagaccaa aagaatgttg cgagcgtaac tttcgctgat aatgagtcag gttaccttgc
     6361 aggtgtggct gcagcaaaaa caactaagac aaaacaagtt ggttttgtag gtggtatcga
     6421 atctgaagtt atctctcgtt ttgaagcagg attcaaggct ggtgttgcgt cagtagaccc
     6481 atctatcaaa gtccaagttg actacgctgg ttcatttggt gatgcggcta aaggtaaaac
     6541 aattgcagcc gcacaatacg cagccggtgc agatattgtt taccaagtag ctggtggtac
     6601 aggtgcaggt gtctttgcag aggcaaaatc tctcaacgaa agccgtcctg aaaatgaaaa
     6661 agtttgggtt atcggtgttg atcgtgacca agaagcagaa ggtaaataca cttctaaaga
     6721 tggcaaagaa tcaaactttg ttcttgtatc tactttgaaa caagttggta caactgtaaa
     6781 agatatttct aacaaggcag aaaaaggaga attccctggc ggtcaagtga tcgtttactc
     6841 attgaaggat aaaggggttg acttggcagt aacaaacctt tcagaagaag gtaaaaaagc
     6901 tgtcgaagat gcaaaagcta aaatccttga tggaagcgta aaagttcctg aaaaataatg
     6961 gatggaagtc atttcttagg aagcggccaa gggccgcttt tttaagagtt gagacaaagt
     7021 cattttgtct cagtaaagct tgctactaga caaactagca agttttattg aaataaaata
     7081 agactctgaa aggaagagca catggcacac gaaaatgtca ttgagatgcg tgatattacc
     7141 aaggtgtttg gtggatttgt tgccaacgac aaaatcaact tgcacctacg aaaaggtgaa
     7201 attcatgcac ttttaggaga aaatggggct ggtaagtcca cgctaatgaa catgttagca
     7261 ggccttcttg aaccaactag tggtgaaatc gcggtcaacg gtcaagttgt caatctcgac
     7321 tccccatcta aagcagctag cttgggaatc gggatggttc accagcactt tatgttggtt
     7381 gaagccttca cagtggctga aaacatcatt ttaggtagtg aattgactaa aaatggtgtg
     7441 ctagatatcg ctggagctag caaagaaatc aaggctcttt ctgaacgtta tggcttagct
     7501 gttgaccctt ctgccaaggt agcagatatc tcagttggag cccaacaacg tgtagaaatt
     7561 ttaaaaacac tttatcgggg ggctgatatc cttatctttg acgaaccaac ggctgttttg
     7621 actccatcag aaattgatga gttgatggct attatgaaaa atcttgtcaa agaaggaaaa
     7681 tcaattatct tgattaccca caaattggat gaaattcgcg cagtttctga ccgtgttaca
     7741 gttatccgtc gtgggaaatc aattgaaacc gttgaaattg caggggctac caatgctgat
     7801 ttggcggaaa tgatggtagg acgttctgtt tcctttaaaa cagagaagca agcctctaaa
     7861 ccaaaagaag tggttttgtc tatcaaagat ttggtggtca atgaaaaccg tggtgttcca
     7921 gctgttaaaa atctgtcctt ggatgttcgt gctggagaga ttgttggtat tgcggggatt
     7981 gatggaaatg gtcagtctga actgattcaa gccattacag gtcttcgtaa ggttgaatct
     8041 ggtagcattg agctaaaagg ggattcaatt gtaggcttgc acccacgtca gattacagaa
     8101 ctaagtgttg ggcacgttcc agaagaccgt caccgtgatg gcttgatttt ggaaatgatg
     8161 atatctgaaa atattgccct tcaaacctac tataaagaac cacatagtaa aaatggaatt
     8221 ttgaattatt caaatattac ttcttatgct aaaaagctga tggaagagtt tgatgttcgc
     8281 gctgccagtg aattagttcc tgcagctgca ctctcaggag gaaatcaaca aaaagcaatt
     8341 attgctcgtg aaattgatcg agatcctgat ctccttatcg ttagccagcc aactcgtggg
     8401 ttggatgtcg gtgccattga gtatatccac aaacgcttga ttgaagagcg tgataatggc
     8461 aaggctgtcc ttgttgtcag ctttgaattg gatgagattt taaacgtctc agaccgtatt
     8521 gccgttatcc acgatggtaa gattcaaggt attgtatcac cagaaacaac caataaacaa
     8581 gaacttggtg tcttgatggc tggtggaaac ttgggaaagg agaagagtga tgtttaaaaa
     8641 attacaacaa atttcggttc ccttgatttc tgtatttcta ggaattttac tcggagccat
     8701 tgtcatgtgg atcttcggtt atgatgctat ttggggctac gaagaattgt tctatacagc
     8761 ctttggcagt ctgcgtggga ttggagaaat cttccgtgct atgggtcctc tggtcttgat
     8821 tggtcttggt tttgcggttg ccagtcgagc tggtttcttt aacgtcggac ttcctggtca
     8881 ggctttggca ggttggattc tcagtggttg gtttgcgctg tcgcatccag atatgccccg
     8941 tcccttgatg attctagcaa ccatcgtgat tgccttgatt gctggtggga ttgtcggagc
     9001 gattccaggt attcttaggg cctatctagg gacgtcagag gttattgtaa ccatcatgat
     9061 gaactacatt gtcttgtatg tagggaatgc ctttatccat gctttcccta aagacttcat
     9121 gcaaagtaca gattcgacca ttcgtgttgg ggctaatgca acctatcaga caccttggtt
     9181 ggctgagttg actggtaact cacggatgaa tattggtatt ttctttgcca tcattgctgt
     9241 tgcagttatt tggttcatgc tcaagaaaac aactcttggt tttgaaatcc gtgcagttgg
     9301 tcttaatcca catgcttcag aatatgctgg tatttctgcc aagcggacta ttatcctatc
     9361 tatgattatt tcaggtgcct tggcaggtct tggtggagct gttgaaggtt tgggaacctt
     9421 ccagaacgtc tatgttcaag gttcgtcatt agctatcgga tttaacggaa tggcggttag
     9481 tttgcttgcg gccaactcac caattggtat actctttgca gccttcctat ttggcgttct
     9541 ccaagttggg gctcctggta tgaatgcggc gcaggtacca tctgagcttg tcagcattgt
     9601 aacagcgtct attatcttct ttgtcagtgt tcattacctt atcgaacgct ttgttaaacc
     9661 gaaaaaacaa gttaaaggag gtaagtaaag atgtctatta taaccttgct ccccctcttg
     9721 gtgtcttcta tgctgattta ctcagcacct ctcatcttta caagtatcgg tggtgttttc
     9781 tctgaacgtg gtggtgtggt aaacgttggc cttgaaggaa ttatggttat gggtgccttt
     9841 tctggagttg tctttaacct tgaatttgca gaacaatttg gagcagcaac tccatggcta
     9901 tccttgcttg tagcaggatt ggttggtagt gttttctcta tcatccacgc agcagcgacg
     9961 gttcatttcc gtgcagacca tgttgtcagc ggtacggtat tgaacttgat ggcgcctgcc
    10021 ttggctgttt tcttagttaa agttctttat aacaaaggac aaaccgacaa cctaagtcaa
    10081 acttttggac gctttgattt cccagtcttg gcaaatatcc cagtgattgg tgatatcttc
    10141 ttcaagtcaa ctagtctact tggttatctg gcgattgcct tctcattcct tgcttggttt
    10201 attctcttta agactcaatt tggtcttcgt ctccgctctg tcggtgaaca tcctcaagca
    10261 gcggatacct tgggaatcaa cgtctacaag atgagatatt taggggttat tatttcaggt
    10321 tttctaggtg gaattggcgg agcgatttat gcgcaatcaa tctcagttaa cttctcagtg
    10381 acaactattg ttggacctgg atttatcgcc cttgctgcga tgatctttgg gaaatggaat
    10441 ccaatcggtg ctatgctttc tagtctcttc tttggacttt cacaaagttt ggctgttatc
    10501 ggttctcaat tgccgtttct acaaggagtg ccagcggttt atcttcaaat tgcaccttat
    10561 gttttgacaa ttcttgtctt agcagccttc tttggaaaag cagtcgcacc aaaagcagat
    10621 ggtatcaact acatcaaatc aaaataagca tacaaaaaaa cgtcagtcca atgactgaac
    10681 tgccccccaa aagttagaca gaaaaaatct aacttttggg gtgtttttat tatgaaatta
    10741 agttatgatg ataaagttca gatctatgaa ctaagaaa
//
LOCUS       DAVMHG010000062        10459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVMHG010000062 DAVMHG010000000
VERSION     DAVMHG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.062"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            236..928
                     /locus_tag="VYR08_001256"
     CDS             236..928
                     /locus_tag="VYR08_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV3466780.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            981..3392
                     /locus_tag="VYR08_001257"
     CDS             981..3392
                     /locus_tag="VYR08_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV3466781.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLNKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            3642..4343
                     /locus_tag="VYR08_001258"
     CDS             3642..4343
                     /locus_tag="VYR08_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466782.1"
                     /translation="MAYIDMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            4345..7053
                     /locus_tag="VYR08_001259"
     CDS             4345..7053
                     /locus_tag="VYR08_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV3466783.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7149..7640)
                     /gene="tpx"
                     /locus_tag="VYR08_001260"
     CDS             complement(7149..7640)
                     /gene="tpx"
                     /locus_tag="VYR08_001260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV3466784.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(7763..8692)
                     /gene="psaA"
                     /locus_tag="VYR08_001261"
     CDS             complement(7763..8692)
                     /gene="psaA"
                     /locus_tag="VYR08_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV3466785.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(8724..9572)
                     /locus_tag="VYR08_001262"
     CDS             complement(8724..9572)
                     /locus_tag="VYR08_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV3466786.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(9569..10291)
                     /locus_tag="VYR08_001263"
     CDS             complement(9569..10291)
                     /locus_tag="VYR08_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV3466787.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3065 a         1969 c         2395 g         3030 t
ORIGIN      
        1 aaaataatga ctcgtttact aaactaaaag agtcaactca aaaattattc gatgcgcaga
       61 aaaaaaggtt aaataatgaa gatagaatag aaacaactaa aaacaatgtg attgccaaac
      121 attgtcaaac agtcctttcc tttttggttt tgactagctt ttttgtgaaa aattgtgtaa
      181 aatagaatag ataaacgagg ggaaacctcg gaaaatttaa aggagaatcc atctaatggt
      241 aaaattggtt tttgctcgcc acggtgagtc tgaatggaac aaagctaacc ttttcactgg
      301 ttgggctgat gttgatttgt ctgaaaaagg tacacaacaa gcgattgacg ctggtaaatt
      361 gatcaaagaa gctggtatca aatttgacca agcttacact tcagtattga aacgtgctat
      421 caaaacaact aacttggctc ttgaagcttc tgaccaattg tgggttccag ttgaaaaatc
      481 atggcgcttg aacgaacgtc actacggtgg tttgactggt aaaaataaag ctgaagctgc
      541 tgaacaattt ggtgatgagc aagttcacat ctggcgtcgt tcatacgatg tattgcctcc
      601 aaatatggac cgtgatgatg agcactcagc tcacacagac cgtcgttatg cttcacttga
      661 cgactcagta atcccagatg ctgaaaactt gaaagtgact ttggaacgtg ctcttccatt
      721 ctgggaagat aaaatcgctc cagctcttaa agatggtaaa aacgtattcg taggagctca
      781 cggtaactca atccgtgccc ttgtaaaaca catcaaaggt ttgtcagatg acgagatcat
      841 ggacgtggaa atccctaact tcccaccatt ggtattcgaa ttcgacgaaa aattgaacgt
      901 cgtttctgaa tactaccttg gaaaataaaa aattgtaagt ctagaattga tttctaggct
      961 ttttatgtta gtatggaagt atgataagga ataaaaaaca agattatgta ctggcctaca
     1021 agcaaccagt ttcaaccact tacatgggtt gggaagaaga agctttaccg ataggcaatg
     1081 gttctttagg agcaaaagta tttggcctta taggggctga acggattcaa tttaatgaaa
     1141 aaagtctctg gtctggaggt ccacttcctg atagttcaga ttatcagggt ggaaatcttc
     1201 aggatcagta tgttttttta gctgagattc ggcaggcttt ggagaagaga gattacaatc
     1261 tggctaagga actggctgag cagcacctaa ttgggccaaa aacgagtcaa tatgggacct
     1321 atctgtcttt tggggatatt cacattgagt tcagccagca aggtacgact ttgtctcagg
     1381 tgacggacta tcagagacag ctgaatatta gtaaggcact tgtgacgact tcttatgtct
     1441 ataagggaac gcgatttgaa cgtgaagctt ttgcgagttt tccagatgat ctcttggttc
     1501 agcgttttac taaggaaggg gcggaaactc tagattttac tatagaacta tccttgacct
     1561 gtgatttggc ttctgatgga aagtatgagc aggaaaaatc tgattacaag gagtgtaagt
     1621 tggatattac tgattctcat atcttgatga agggaagagt taaggataat gatctgcggt
     1681 ttgctagtta tctagcttgg gaaacggatg gagatattag agtttggtca gatagggttc
     1741 agatatcagg agccagttat gccaatctct tcttggccgc taagacggat tttgcccaaa
     1801 atcctgctag caattatcgc aagaaactag atttagagca acaggtgata gacttggtgg
     1861 acacagctaa agaaaagggc tatacccaat tgaaatcaag gcatatcgag gactaccaag
     1921 ccttattcca gcgtgttcaa ttggatttgg aagctaatgt tgacgcattc actacagatg
     1981 atttgttaaa aaattataag ccacaagaag ggcaggcttt ggaggagctg ttcttccagt
     2041 atggacggta tttattgatt agttcgtcca gagactgccc agatgctcta ccagctaacc
     2101 tacagggagt ctggaatgcg gtcgacaatc ctccttggaa ttcggactat cacttaaatg
     2161 tcaatctgca gatgaattat tggccagcct atgttaccaa tctcctagag gcggtctttc
     2221 cggtcatcaa ctatgtagat gatttgcgtg tctatggtcg tctagcggct gtaaagtatg
     2281 caggaatcgt ctctcaaaaa ggtgaggaaa atggttggtt ggttcatact caagcgactc
     2341 cctttggttg gacggcacct ggttgggatt actattgggg ttggtcacca gctgccaatg
     2401 cgtggatgat gcaaactgtt tatgaagcct attcatttta tagggaccaa gactatctca
     2461 gggagaaaat ttatcccatg ttgagggaaa cggttcgttt ttggaatgcc tttttaaata
     2521 aggatcagca ggcgcagcgt tgggtgtctt ctccgtctta ttccccagaa catgggccga
     2581 tttcgattgg caatacttat gaccaatctc tgatttggca gttatttcat gattttattc
     2641 aggctgctca ggaattggga ctggatgagg acttgttgac tgaggttaag gagaagtctg
     2701 atttactaaa tcctttgcaa atcactcaat ctggtcgaat cagggagtgg tatgaggagg
     2761 aagagcagta ttttcaaaat gagaaagtgg aggcccagca tcggcacgct tcccatctag
     2821 tgggactcta tcctggcaat ctctttagct acaagggaca agagtatatt gaagcggcac
     2881 gtgctagcct caatgatcgt ggagatggcg gcacaggctg gtccaaggct aataagatca
     2941 atctctgggc gcgtttggga gatggcaatc gagcccataa attattggca gagcagttaa
     3001 aaacatccac cttgccaaat ctttggtgta gccatcctcc ttttcagata gatggtaatt
     3061 ttggtgctac tagtggcatg gcagaaatgt tactccagtc tcatgcagct tatctggtac
     3121 ctctagctgc cctacctgat gcttggtcaa caggttctgt ttcaggctta atggcacgtg
     3181 gacattttga agtgagcatg agctgggaag ataaaaaact cttacagttg accattttat
     3241 caaggagtgg aggagatttg cgagtttctt atccagatat tgagaagagt gtgattaaaa
     3301 tgaatcaaga aaaaataaaa gcgaaatgca tggggaaaga ttgtatttcg gtggcaacag
     3361 cagaaggtga tcttgttcaa ttttattttt aagaagatgt tataaggcag taatttgaaa
     3421 cttcctttta ataaggattt aagaatatag gcagtttcca actagttgaa aaagcgttat
     3481 aatgataata ggaagtaata ctcaatgaaa atcaaagagc acaaactagg aagctagccg
     3541 caggttgctc aaaacagtgt tttgaggttg tagatggaag ctgacgtggt ttgaagagag
     3601 attttcgagg agtataattt gtttgataga gggtgggtct gatggcttat attgatatga
     3661 aacactgtta caagcgttat caggttgggg acacggagat tgtggccaat cgtgatgtga
     3721 attttgagat tgaaaagggg gagctggtta ttatccttgg tgcttcaggt gcaggcaagt
     3781 caacagttct taaccttctt gggggaatgg ataccaatga tgaaggggaa atctggattg
     3841 atggtgttaa tattgcggat tatagttccc accagcgcac caattaccgt agaaatgatg
     3901 tggggtttgt ttttcagttt tataatctag tttctaatct gacagctaag gaaaatgtgg
     3961 aactggcttc tgaaattgtg acagatgcct tgaatcctga tcaggtcttg acagatgtag
     4021 gtctggctca tcgtctcaat aactttccag cccagctttc tggaggggag caacagcgag
     4081 tctccattgc acgcgcggta gccaaaaatc ctaaaattct cctttgtgat gaaccgactg
     4141 gagccttgga ttatcagacg ggcaagcagg ttttgaaaat tctccaagac atgtctcgtc
     4201 aaaagggagc gacggtgatc atcgtgactc ataatggagc tttggcgccc attgctgatc
     4261 gcgtgattca tatgcacgat gccagtgtca aggatgtggt gctcaaccag catcctcagg
     4321 atattgacag tttggagtac tagcatgatc aagcgaaaaa cttattggaa ggacttaatt
     4381 cagtccttca cagactccaa ggggcgtttt ttatccatct tgatcctgat gatgttggga
     4441 tctctagcct tagtaggcct caaagtaacc agtcccaaca tggaggcgac agctaatgct
     4501 tatttaacaa ctgctcaaac cttggatttg gcagtcatgt ctaactatgg cttggatcaa
     4561 gcagaccaag aagaactaaa acagacggag ggcgcagagg tcgagtttgg ctatttgaca
     4621 gatgtgacta tggataatgg gcaggatgcc attcgactgt actccaaacc agagcgaatt
     4681 tcaacctttc agctaagaaa gggacgactt cctcagtcag acagggaaat cgctttggcc
     4741 actcatttgc aaggccaata cagcgtggga caggagatta gttttaaaga aaaagaagag
     4801 ggtcattcct ctttaaaaga ccatacttat accattactg gttttgtgga ttcggctgaa
     4861 atcctctccc agcgagatat gggctacgca ggaagtggaa gtgggactct gacagcctat
     4921 ggggtgattt tacctagtca atttgatcag aaagtctaca atatagctcg tttgaaatat
     4981 caagatttag caggtttaaa tgccttttca tcagcttatg aagaaaaatc caagcaacat
     5041 caggaagacc ttgaacaaac tttatcagat aatggcaagg tacgtctgca acttttgaaa
     5101 aaagaaggac aagagtctct agacaagggg caagagactc ttgacaaggc tcagactaat
     5161 ttgcaggaag gcaagcgtcg tttagcagct gctcaagctc gtatacaggc tcaagaaagt
     5221 caactagcct tgtttcctca agctcagaga gagcaggcta gtgctcaact tacccaagcc
     5281 aagcaggaac tgggcaagga agaggacaaa ctaaagcaag ctgaacaaaa tctagcccaa
     5341 gaaaaggaaa aattagaaaa acatcagcaa gtcttggatg atttggcgga gccaaggtat
     5401 caggtttata atcgtcagac catgccaggt ggtcagggct atcttatgta tagcaatgct
     5461 tcatccagta ttcgagcagt gggcaatatc tttcctgtgg tactttatgc cgtagcagcc
     5521 atggtgacct ttacgaccat gactcgcttt gtagacgaag agcgaactca tgcagggatt
     5581 tttaaggcct tgggttatcg tagtaaggat attatcgcca agtttctcct ttatggacta
     5641 gtagctggga ctgtcggaac ggctctaggt agtatacttg gtcattattt gctagccagt
     5701 gtaatttcaa gtgtcattac aaaaggcatg gtggtgggag aaacccagat tcagttctat
     5761 tggacctata gcttactagc tcttgtcttg agctggttgg cgagtgtgtt accagcctat
     5821 ctggtggctt ggagggaact tcatgacgaa gcagcccagc ttctacttcc taaacctcct
     5881 gtcaaaggag ctaaaatctt attggagcgt atcggtttta tctggcgtcg tctcagtttt
     5941 actcataagg taacagcccg caacatcttt cgttataagc agagaatgtt gatgacaatc
     6001 tttggtgtgg caggttctgt agctctgctc tttgcaggtt tgggaatcca atcttctgta
     6061 gcaggagttc cgtctaaaca gtttcaacaa atccaacagt atcagatgct tgtctctgaa
     6121 aatcctagtg cgaccaatca ggacaaggta gagctagcag aagtgttgaa agggcaggag
     6181 atactagcct accagaaaat ctattctaaa acgctagaca aggatttcaa gggcagggct
     6241 ggtcttcaaa acattactct tatgatgata gagaaggaag atttgactcc ctttatccat
     6301 cttcaacatc atcagcagga gctgacatta aaagatggca tcgttattac agctaaactc
     6361 gcccagctgg caggtgtcaa ggttgggcag actttagaaa ttgaaggtaa ggaactaaag
     6421 gtcgctgcta ttactgagaa ctacgttggt cactttattt atatgagtca ggctagctat
     6481 gagcaacttt acggacagct accccaagcc aacacttatc tggtctcatt aagggatacc
     6541 agtgcaacta gtatcgaaag tcaggcgggc ttgcttatga atcaatctgc ggtgtccagt
     6601 gttgtccaaa atgcttcagc cattcgactc ttcgactcta tcgctagctc actcaatcag
     6661 accatgacca tcttggtcat cgtatcggtt ctattagcta ttgtcatcct ttacaatctg
     6721 accaatatca acgtagctga gagaatccgt gaactctcca ctatcaaggt tctcggtttt
     6781 cataataatg aagtcaccct ctacatttac cgtgagacga ttgtgctgtc ccttgtggga
     6841 atcgtacttg gtctggtagc tggtttctat ttacaccaat ttttgattca aatgatttcg
     6901 cctgcgacta ttctctttta tccgcaggta ggctgggaag tctatgtaat cccagtggca
     6961 gcagtaagca tcattttgac cttgcttggt ttcttcgtca attattatct gagaaaggtt
     7021 gacatgttag aagccctgaa atctgtagag taaggtagtt atttttagct gattgaactt
     7081 ctatttacta atattcaaaa atcctccgtt tcaaagagca gggaactctt tgtgacagag
     7141 gattttttct atagggcttt agcagctgca attgcggctt cgaagtttgg ctcagaattg
     7201 atattatcca cgtattcaac gtagcgaatc gtattgtcag tatcgaggac aaagactgcg
     7261 cgtgctaata ggtgccattc gttgatcaag agggcataat cgcgtccgaa agaatggtca
     7321 aagtagtctg aaagcataat ggcattgtca aggccttcag caccgcacca acgtttttga
     7381 gcaaaaggta ggtccattga aacagtcaat acgaccgtgt tgtccagtcc agccaattct
     7441 tcattaaaac gacgtgtttg agttgagcag atgcctgtat cgatagaagg aacgacactc
     7501 aagacttttt tcttgccatc aaaatcagcc agagattttt tagaaagatc tgttgtagta
     7561 agagaaaaat caagcgcctt gtcgccgact tgtagttgtt tacctgtaaa gctcacagga
     7621 tttccgagaa aagttaccat aggatactcc aatctttttt cttccattgt atctgaaaca
     7681 gtcagaattt tccaatgatt tgaccggaaa tgtgtgcata gaaaaaacgc cagctcacat
     7741 gagaatgacg tttttcagag gcttattttg ccaatccttc agcaatcttg tcaaggttgt
     7801 atttcatcat gctgtagtag ctgtcgcctt ctttaccttg ttctgcgata gagtcagtaa
     7861 agatttgtgc gtagattggg atgtttgtgt cttgagaaac agttttcatt ggacggtcat
     7921 ccacacttga ttctacaaag agtgatggaa cttttgtttg gcgaagtttt tcaaccaagg
     7981 tcttgatttg ttcaggagtt ccttcttctt cagtattgat ttcccagatg taggcacttg
     8041 ggacaccata ggctttagag aagtatttga atgctccttc gctggttaca atgagtttct
     8101 tttcagcagg gatcttatta aatttatcct tactttcttt atcaagtttg tctaacttat
     8161 cagtatattc tttgagattt ttttcataga attctttatt gttagggtct ttggcgctca
     8221 attgtttggc gatattttta gcaaaaataa taccgttttc aaggttaagc caagcgtgtg
     8281 ggtcttcttt tcctttttca ttttgacctt caaggtagat aacatcaacg ccgtcgctga
     8341 ctgcgaagta gtctttgttt tcagttttct tggcattttc taccaatttt gtaaaccaag
     8401 cattgccacc tgtttcaagg ttgataccgt tatagaaaat caaatcagcc tcagaagttt
     8461 tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc ttgcccaatc ggaacgatac
     8521 tatgaaggtc aattttgtca ccagcaatat ttttagtaat atcagcgatg attgagtttg
     8581 tagcaacaac ttttagtttt tgaccagaag ttgtatcttt ttttccgcta gcacatgcta
     8641 caagaatgat tgcagaaaga aagagaacga gtaatgtacc taattttttc attagatcct
     8701 ccaatttatt agggctttgc cccttatttt aacaaatgtt tatttttcag tttcaaatat
     8761 cgttgtttgg gagcgataaa gaagctaatg agaaagaaac tagcagctgt aagcacgata
     8821 ctagaacctg ccgcaacatt aaaactatag ccaataaaga gtcccaaaac tgaagcagta
     8881 gctccgaagg ttgaggaaag gaaaatcatg cttttcagac tattagcata cagataagca
     8941 gttgcagctg gggtaatcag catggctaca atcaggatag ttccgacact ttgcatggct
     9001 gtcacagaca cgagagtcag gagtaccatg agaaggtagt gatagaaatt gacaggcatt
     9061 cccatggctt tagccaagag ttcatcaaag gaagttatca agagttgctt gaagaaaatc
     9121 cagattaaca agaggatggc tgcccccaca cccatagtaa taaacatatc cgtatcttgg
     9181 acggccagaa tattaccaaa aaggatatgg aaaaggtcag ttgaactttt agcgacacca
     9241 atcaagatga taccgagggc taagaaagaa gaaaaggtaa tgccgatggc ggtatcgctt
     9301 ttgataatcg agtttccttt gatgtaggta atgatgatgg cagctagcaa tccaaagaca
     9361 atggctccga taaagaagtc aaggcccaag atgaaggaga gggctacacc tggtaagaca
     9421 gcatgtgaaa tggcatctcc catgagtgac atcccgcgta gaataatgaa acatcccacg
     9481 gctccagcta caatcccgac gacaatagct gttatcaagg cattttgtag gaaatggaat
     9541 ttttgcaatc catcgataaa ttctgcaatc ataggtcacc tccattgaaa aagagttgat
     9601 taccgtaagc ttcttttaga ttggtttcgg taaaagtttc ttttgttgga ccaaaggcaa
     9661 tcacttctcg attgacaagt aagacttgat cgaagtagtg gggaatcttg ctgaggtcgt
     9721 ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc tctcagcgta ttcatgatga
     9781 tttcctcact gacagagtca atcccagcaa agggttcatc caagaggata tagtcggctt
     9841 cctgcaccaa acatctggca atcaagaccc gctggaattg acctccagac agttgactaa
     9901 tttgacgttc agcgtagtca gctaggccga cgatttcaag ggcctcttgc actttcttcc
     9961 aatgtttagc ctttaaactt cgaaagagag gaatagaggg aaatagtcct aacgagacgc
    10021 attccttgac cttgatggga aagttgtagt cgatattgat tttttgttcg acataggcaa
    10081 ttcggtgtaa ggatttttta acttccttgt catcgagaaa tgcctgacct tgatgtggga
    10141 taattcccag catacctttt aatagtgttg atttcccagc gccgtttgga ccaatgatgc
    10201 cggtaattgt tggtccatgg agcactagtg aaatatcctt aagtgccaac gtttctttgt
    10261 aggagacact gaggttttcg atacgtatca taaacttgta ttcctcctgt ctcttaatat
    10321 acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa attaaaataa taactgaaaa
    10381 atagattcta aagataactt tcaggataaa tttctaaatt ataaaacgca tagtatgcat
    10441 agtatcaagt gtaaaaaac
//
LOCUS       DAVMHG010000063        10296 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMHG010000063 DAVMHG010000000
VERSION     DAVMHG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.063"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            18..272
                     /gene="blpM"
                     /locus_tag="VYR08_001264"
     CDS             18..272
                     /gene="blpM"
                     /locus_tag="VYR08_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV3466788.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATSGF"
     gene            288..491
                     /gene="blpN"
                     /locus_tag="VYR08_001265"
     CDS             288..491
                     /gene="blpN"
                     /locus_tag="VYR08_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV3466789.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            735..968
                     /locus_tag="VYR08_001266"
     CDS             735..968
                     /locus_tag="VYR08_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /protein_id="HEV3466790.1"
                     /translation="MDTKMMSQFSVMDNEMLVRTEDGDVSDIYRGYANQRSPFASYPS
                     ILKNSGPFPVSGYCLRGYHDRGYIGAGFHLCGI"
     gene            1000..1689
                     /locus_tag="VYR08_001267"
     CDS             1000..1689
                     /locus_tag="VYR08_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3466791.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVVVLHISFNVQTAKH
                     VQSTAEGWNVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1731..1964
                     /locus_tag="VYR08_001268"
     CDS             1731..1964
                     /locus_tag="VYR08_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466792.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            2115..2726
                     /locus_tag="VYR08_001269"
     CDS             2115..2726
                     /locus_tag="VYR08_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3466793.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLIFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2887..3681
                     /locus_tag="VYR08_001270"
     CDS             2887..3681
                     /locus_tag="VYR08_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV3466794.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3678..4313
                     /gene="trmB"
                     /locus_tag="VYR08_001271"
     CDS             3678..4313
                     /gene="trmB"
                     /locus_tag="VYR08_001271"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV3466795.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4439..4918
                     /gene="rimP"
                     /locus_tag="VYR08_001272"
     CDS             4439..4918
                     /gene="rimP"
                     /locus_tag="VYR08_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV3466796.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4962..6098
                     /gene="nusA"
                     /locus_tag="VYR08_001273"
     CDS             4962..6098
                     /gene="nusA"
                     /locus_tag="VYR08_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV3466797.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6120..6413
                     /locus_tag="VYR08_001274"
     CDS             6120..6413
                     /locus_tag="VYR08_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV3466798.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6406..6705
                     /locus_tag="VYR08_001275"
     CDS             6406..6705
                     /locus_tag="VYR08_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV3466799.1"
                     /translation="MNKQKISNLLGLAQRAGRIILGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6722..9514
                     /gene="infB"
                     /locus_tag="VYR08_001276"
     CDS             6722..9514
                     /gene="infB"
                     /locus_tag="VYR08_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV3466800.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAADPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPYKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9765..10115
                     /gene="rbfA"
                     /locus_tag="VYR08_001277"
     CDS             9765..10115
                     /gene="rbfA"
                     /locus_tag="VYR08_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV3466801.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKVQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3140 a         1882 c         2386 g         2888 t
ORIGIN      
        1 aaaaaagagt atttgtcatg gatacaaaaa ttatggaaca atttcatgaa atggatataa
       61 ctatgttatc tagtattgaa ggaggcaaga ataattggca aactaatgtc ttagaaggtg
      121 gtggtgctgc ttttggtggc tgggggttag ggacagctat ttgtgctgcg agtggtgttg
      181 gagcgccatt tatgggagca tgtggataca taggagctaa atttggtgtg gctctttggg
      241 caggagtaac aggggcaacg agtggatttt aacaaaagga gacttgtatg aatacttatt
      301 gtaatataaa tgaaacaatg ctgagtgagg tttatggagg taattccgga ggagcagctg
      361 tagttgctgc tttaggttgt gcagcgggtg gagtgaaata tgggagactt ctaggaccat
      421 ggggcgctgc aataggagga attggaggag cagtggtttg tggatattta gcctataccg
      481 ctacatcata aaaatgaaag ctaaatttta tgaaggctat attctttatt attctcttcg
      541 cttttcaaac ctatcttatt tatctatcca taagtatctc cgataaaaaa caaaagacta
      601 ttgaattaac atcattgaat tgttttgtca tattatttct aatctatgat aaattaattt
      661 ttctctttat tgcttatgtt tttttgataa tttttatatt aaacttgttc cgtaactgaa
      721 aagtgatata ctatatggat acaaaaatga tgtcacaatt ttctgttatg gataatgaaa
      781 tgcttgttcg tacagaggat ggggatgtct ctgatattta tagagggtac gctaatcaga
      841 gaagtccatt cgcttcttac ccttctatat taaaaaattc aggtcctttt ccagtaagtg
      901 ggtactgcct gcgtggttat catgaccgtg gttatatagg agcaggtttt catttatgtg
      961 gaatataggc tgaaatacaa gaaatgaggg aggatttcta tgaaaaagta tcaacttcta
     1021 ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttggtctttc tcagctgacg
     1081 cttatcgtcc aaaattattg gcaattttct tcccagattg gcaatttcgt ctggattcaa
     1141 aatatcttga gtttactatt tagcggagtc atgatttgga ttctggttaa gacaggccat
     1201 ggctatctct ttcgcattcc aagaaaaaaa tggctttggt attctatttt gacagtatta
     1261 gtggtagtgc tccatatctc ttttaacgtt cagacagcta aacatgttca gtcaactgct
     1321 gagggttgga acgtattgat tggttatagt gggaccaact ttgctgagct aggtatctat
     1381 gtaaccctgt ttttcctgac tccactaatg gaagagttga tttatagggg attacttcaa
     1441 cacgccttct ttaagcattc gcgatttggc cttgatttgc ttcttccttc cattttgttt
     1501 gctcttcctc attttttgag tctgcctagt ctgttagata tcttcgtctt tgcaacattt
     1561 ggaatcatct ttgccggttt gacccgctat accaagagta tttatccatc ctatgcggtg
     1621 catgtgatca ataatattgt agcgaccttc ccgtttttgc tcacttttct acatagggtc
     1681 ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc atgtataaac
     1741 acttattttt cctagattcc aaaactttag atcggttgac accctatatt ctagtcttgg
     1801 cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg gtggttttta
     1861 atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc tatgtggtcg
     1921 gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
     1981 tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc gaagagtata
     2041 aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt ctgtttgttg
     2101 aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt ggatttttag
     2161 tactaataat tgtcattaca gttccttata cgattaacca tggggatttt tttcaaaatg
     2221 aatctgcatt gattattgta agtcttcttg taacctcgct gagtgttgct tatgctagaa
     2281 agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgatt ttcattgcaa
     2341 tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct gtttcttctg
     2401 gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct ttgattttga
     2461 ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt cttcaaggtg
     2521 cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt tctttcatgc
     2581 atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg ttgctgggcc
     2641 ttgcttataa aaagagccaa aacctatggg tttctactct agtccacatg ttttacaaca
     2701 gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag acggtgctta
     2761 cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg agaatcgggt
     2821 tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt tatacggagg
     2881 aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg gaaaagtggc
     2941 aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat gaacacctct
     3001 ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg gagtcgtcgt
     3061 ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat attgaccccc
     3121 tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg ctcacgtccg
     3181 ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga tttactacag
     3241 tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag tgaagtgatg
     3301 gctgatttgc gtcagactat tccaggattt agagaggacc atgcgaccat tgtccatgga
     3361 gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt agtagattgg
     3421 gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg ccattatatt
     3481 tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa tcaaacggta
     3541 ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc caagtattat
     3601 atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca tttccgagac
     3661 aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa ttactagagg
     3721 caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg cgggacttgt
     3781 ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc tttgtttcag
     3841 gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa aagtctgttt
     3901 tgagctacgc tttggacaag gtgcttgaag ctggagtgtc taacatcaag ctcttgtggg
     3961 tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc ttgtatctga
     4021 acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc tacaagacct
     4081 ttttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc aagacggata
     4141 accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg aaactcaatg
     4201 gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca gaatacgagc
     4261 aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt taagagatag
     4321 cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg tgctatactg
     4381 atggtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt atgaggaggt
     4441 ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg tcatagaagc
     4501 tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga ttctcagtat
     4561 ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttaa cagaaatgat
     4621 cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt tcctagaaat
     4681 taccagtcca ggcttggaac gtcctttgaa aaccaaggat gccgtcgctg gagcggttgg
     4741 aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct ttgaaggaac
     4801 cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga cgcgtaagaa
     4861 aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta aattatagaa
     4921 aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa gaaatgctag
     4981 aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc atcgacgcag
     5041 tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac agcgtagcta
     5101 ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa gttgttgatg
     5161 aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt aattcagctt
     5221 atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt cgtgtagcag
     5281 cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt gccatcactt
     5341 acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa cgctttgaca
     5401 accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa caagaccaaa
     5461 ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac aaggttgaag
     5521 acaaccctcg tggtgtgaac gtctttgtta gccgtagcca tccagaaatg atcaaacgtt
     5581 tgatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg agcgtggctc
     5641 gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac gtggatgcta
     5701 tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc aaattccacc
     5761 cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat atcgatgtta
     5821 tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct gctgaggttg
     5881 accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt ccagataaca
     5941 agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct cacttgactg
     6001 gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac gctgcttcag
     6061 tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag gagggaaaga
     6121 tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg attgataagc
     6181 gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat cctacgggca
     6241 aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta gaggcgaaaa
     6301 agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt tatgacgagt
     6361 tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa taagcaaaag
     6421 ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatattggg tgaagaattg
     6481 gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca tgatgctgga
     6541 cccaatctga ccaagaagat tcaagataaa agtcattatt atcaagtaga aattgtaacc
     6601 gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt tttggctgta
     6661 acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag gaggacatga
     6721 tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa gtaaagaagt
     6781 tgtagcgcgt gcaaaagagt taggcttgga tgtgaaaagc cactcatcaa gtgtggaaga
     6841 agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgatccga aagtagaagc
     6901 aaaacctgca gcaccaaaag taagtgcaga aaagaaagcc gaaaaatctg agccagctaa
     6961 accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga aaacagaaaa
     7021 agtagcagtg aaaccgcaaa gtcgtaattt caaggctgag cgtgaagccc gtgctaaaga
     7081 gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac aaaacggaaa
     7141 ccgtcagaaa aacgacggcc gtaatggtgg aaaacaaggt caaagcaacc gcgacaatcg
     7201 tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa atgagcgccg
     7261 tcagcaagag gacaaacgtt caaatcaagt ggctccacgt attgacttta aagcccgtgc
     7321 agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg aacgcttcaa
     7381 gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg aaccagagga
     7441 aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag cagtggttga
     7501 agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac aagctcgacc
     7561 atacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac aacaaaagaa
     7621 tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata acaacaaaaa
     7681 gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg ttacggagcg
     7741 taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg ttgcggaaat
     7801 cgcaaaacgt atcaaacgtg aaccagctga aattgttaag aaacttttca tgatgggtgt
     7861 catggccaca caaaaccaat ccttggatgg ggaaacaatt gaactcctca tggtggatta
     7921 cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac gtttctttgt
     7981 cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg ttactatcat
     8041 gggacacgtt gaccacggta aaacaaccct tttggatact cttcgtaact cacgtgttgc
     8101 gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg tggaaaatgg
     8161 taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa tgcgtgcgcg
     8221 tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg gggttatgcc
     8281 tcagactatc gaagccatta accactcaaa agcggccaac gttccaatca tcgtagccat
     8341 taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat tggcagagca
     8401 tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct cagctaaatt
     8461 caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa tccaagaact
     8521 taaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct tggataaagg
     8581 aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc aagacccaat
     8641 cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg gtcgtcgtgt
     8701 taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag caccgatggc
     8761 gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag gtgaagagcg
     8821 tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc ttgaaaacct
     8881 ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca aggctgatgt
     8941 acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg aaggtgtcaa
     9001 agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga cccttgccga
     9061 agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag ctcgtcaaca
     9121 agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg ttatcgaaga
     9181 gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag ttattggtga
     9241 agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg gatttatggt
     9301 tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg gtgtcgttat
     9361 ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg aagttacaaa
     9421 cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg atgatgtgat
     9481 tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt cttaggtggt
     9541 gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg tctcaaaaat
     9601 cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa agaagcagat
     9661 gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta aaatagctag
     9721 gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca aatcatttcc
     9781 gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg caaaagaaag
     9841 tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgctg ggtgacttgt
     9901 ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac caaaaagtcc
     9961 aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc aatttgaaat
    10021 tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat ggaaacaaga
    10081 ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc cctctttttt
    10141 ggtggaggaa aataggttga atttgaaatg gaaaaatatt cttttataat agattgaaac
    10201 tagaatagta cgcctctact tctaaaatat tgttagaaat cgatttgact gtcctgatcg
    10261 atttgtcctg ttcttgtttc gttttaatat aaaaaa
//
LOCUS       DAVMHG010000064        10254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMHG010000064 DAVMHG010000000
VERSION     DAVMHG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.064"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            51..4991
                     /locus_tag="VYR08_001278"
     CDS             51..4991
                     /locus_tag="VYR08_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV3466802.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETAEAASAAKPEEK
                     VGEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFSDVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5081..6277
                     /locus_tag="VYR08_001279"
     CDS             5081..6277
                     /locus_tag="VYR08_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV3466803.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6413..6940
                     /locus_tag="VYR08_001280"
     CDS             6413..6940
                     /locus_tag="VYR08_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3466804.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7017..7373
                     /gene="glnR"
                     /locus_tag="VYR08_001281"
     CDS             7017..7373
                     /gene="glnR"
                     /locus_tag="VYR08_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV3466805.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7410..8756
                     /gene="glnA"
                     /locus_tag="VYR08_001282"
     CDS             7410..8756
                     /gene="glnA"
                     /locus_tag="VYR08_001282"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV3466806.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8852..8971)
                     /locus_tag="VYR08_001283"
     CDS             complement(8852..8971)
                     /locus_tag="VYR08_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV3466807.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9459..10254
                     /locus_tag="VYR08_001284"
                     /pseudo
     CDS             9459..10254
                     /locus_tag="VYR08_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3338 a         1840 c         2218 g         2858 t
ORIGIN      
        1 tataaagcgc tttcttgaaa acaacaaaat caatctttta ggaggagaaa atgaagaatc
       61 cattttttga aagacgttgt cgttacagta ttcgtaagtt atcagtagga gcctgctcgc
      121 tgatgattgg tgctgtttta tttgctggtc cagccttggc tgaagaaact gcagttcctg
      181 aaaatagcgg agctaataca gagcttgttt caggagagag tgagcattcg accaatgaag
      241 ctgataagca gaatgaaggg gaacatgcta gagaaaacaa gctagaaaag gcagaaggag
      301 tagcgacagc atctgaaact gcagaagcag ctagcgcagc taaaccagag gaaaaagtag
      361 gtgaggtggt tgcagaaaca ccatctgcag aagcaaaacc taagtctgac aaggaaacag
      421 aagcaaagcc cgaagcaact aaccaagggg atgagtctaa accagcagaa gaagctaata
      481 agactgaaaa agaagtccag ccagatgtcc ctaaaaatac agaaaaaaca ttaaaaccaa
      541 aggaaatcaa atttaattct tgggaagaat tgttaaaatg ggaaccaggt gctcgtgaag
      601 atgatgctat taaccgcgga tctgttgtcc tcgcttcacg tcggacaggt catttagtca
      661 atgaaaaagc tagcaaggaa gcaaaagttc aagccttatc aaacactaat tctaaagcaa
      721 aagaccatgc ttctgttggt ggagaagagt tcaaggccta tgcttttgac tattggcaat
      781 atctagattc aatggtcttc tgggaaggtc tcgtaccaac tcctgacgtt attgatgcag
      841 gtcaccgtaa cggggttcct gtatacggta cactcttctt caactggtct aatagtattg
      901 cagatcaaga aagatttgct gaagctttga agcaagacgc agatggtagc ttcccaattg
      961 cccgtaaatt ggtagacatg gccaagtatt atggctatga tggctatttc atcaaccaag
     1021 aaacaactgg agatttggtt aaacctcttg gagaaaagat gcgccagttt atgctctata
     1081 gcaaggaata tgctgctaag gtaaaccatc caatcaagta ttcttggtat gatgccatga
     1141 cctataacta tggacgttac catcaagatg gtttgggaga atacaactac caattcatgc
     1201 aaccagaagg agataaggtt ccggcagata acttctttgc taactttaac tgggataagg
     1261 ctaaaaatga ttacactatt gcaactgcca actggattgg tcgtaatcct tatgatgtat
     1321 ttgcaggttt ggaattgcaa cagggtggtt cctacaagac aaaggttaag tggaatgaca
     1381 ttttagacga aaatgggaaa ttgcgccttt ctcttggttt atttgcccca gataccatta
     1441 caagtttagg aaaaactggt gaagattatc ataaaaatga agatatcttc tttacaggtt
     1501 atcaaggaga ccccactggc caaaaaccag gtgacaaaga ttggtatggt attgctaacc
     1561 tagttgcgga ccgtacgcca gcggtaggta atacttttac tacttctttt aatacaggtc
     1621 atggtaaaaa atggttcgta gatggtaagg tttctaagga ttctgagtgg aattatcgtt
     1681 cagtatcagg tgttcttcca acatggcgct ggtggcagac ttcaacaggg gaaaaacttc
     1741 gtgcagaata tgattttaca gatgcctata atggcggaaa ttcccttaaa ttctctggtg
     1801 atgtagccgg taagacagtt caggatgtga gactttattc tactaagtta gaagtaactg
     1861 agaagaccaa acttcgtgtt gcccacaagg gaggaaaagg ttctaaagtt tatatggcat
     1921 tctctacaac ttcagactac aaattcgatg atgcagatgc atggaaagag ctaacccttt
     1981 ctgacaactg gacaaatgaa gaatttgatc tcagctcact agcaggtaaa accatctatg
     2041 cagtcaaact atttttcgag catgaaggtg ctgtaaaaga ttatcagttc aacctcggac
     2101 aattaactat ctcggacaat caccaagagc cacaatcgcc gacaagcttt tctgtagtga
     2161 aacaatctct taaaaatgcc caagaagcgg aagcagttgt gcaatttaaa ggcaacaagg
     2221 atgcagattt ctatgaagtt tatgaaaaag atggagacag ctggaaatta ctaactggct
     2281 catcttctac aactatttat ctaccaaaag ttagccgctc agcaagtgct cagggtacaa
     2341 ctcaagaact gaaggttgta gcagtcggta aaaatggagt tcgttcagaa gctgcaacca
     2401 caacctttga ttggggtatg actgtaaaag ataccagcct accaaaacca ctagctgaaa
     2461 atatcgttcc aggtgcaaca gttattgata gtactttccc taagactgaa ggtggagaag
     2521 gtattgaagg tatgttgaac ggtaccatta ctagcttgtc agataaatgg tcttcagctc
     2581 agttgagtgg tagtgtggat attcgtttga ccaagccacg taccgttgtt agatgggtca
     2641 tggatcatgc aggggctggt ggtgagtctg ttaacgatgg cttgatgaac accaaagact
     2701 ttgaccttta ttataaagat gcagatggtg agtggaagct agctaaggaa gtccgtggta
     2761 acaaagcaca cgtgacagat atcactcttg ataaaccaat cactgctcaa gactggcgct
     2821 tgaatgttgt cacttctgac aatggaactc catggaaggc tattcgtatc tataactgga
     2881 aaatgtatga aaagcttgat actgagagtg tcaatattcc gatggccaag gctgcagccc
     2941 gttctctagg caataacaag gtacaagttg gcttttcaga tgtaccggct ggagcaacta
     3001 ttactgttta tgataatcca aattctcaaa ctccgcttgc aaccttgaag agcgaagttg
     3061 gaggagacct agcaagtgca ccattggatt tgacaaatca atctggtctt ctttattatc
     3121 gtacccagtt gccaggcaag gaaattagta atgtcctagc agtttccgtt ccaaaagatg
     3181 acagaagaat caagtcagtc agcctagaaa caggacctaa gaaaacaagc tacgccgaag
     3241 gggaggattt ggaccttaga ggtggtgttc ttcgagttca gtatgaagga ggaactgagg
     3301 acgaactcat tcgcctaact cacgcaggtg tatcagtatc aggttttgat acgcatcata
     3361 agggagaaca gaatcttact ctccaatatt tgggacaacc ggtaaatgct aatttgtcag
     3421 tgactgtcac tggccaagac gaagcaagtc cgaaaactat tttgggaatt gaagtaagtc
     3481 aggaaccgaa aaaagattac ctagttggtg atagcttaga cttgtctgaa ggacgctttg
     3541 cagtggctta tagcaatgac accatggaag aacattcctt tactgatgag ggagttgaaa
     3601 tttctggtta cgatgctcaa aagactggtc gtcaaacctt gacgcttcat taccaaggcc
     3661 atgaagttag ctttgatgtt ttggtatctc caaaagcagc attgaacgat gagtacctca
     3721 aacaaaaatt agcagaagtt gaagctgcta agaacaaggt ggtctataac tttgcttcat
     3781 cagaagtaaa agaagccttc ttgaaagcaa ttgaagcggc cgaacaagtg ttgaaagacc
     3841 atgaaactag cacccaagat caagtcaatg accgacttaa taaattgaca gaagctcata
     3901 aagctctgaa tggtcaagag aaatttacgg aagaaaagac agagcttgat cgcttaacag
     3961 gtgaggttca agaactcttg gctgccaaac caaaccatcc ttcaggttct gccctagctc
     4021 cgcttcttga gaaaaacaag gccttggttg aaaaagtaga tttgagtcca gaagagctta
     4081 caacagcgaa acagagtcta aaagatctgg ttgctttatt gaaagaagac aagccagcag
     4141 tcttttctga tagtaaaaca ggtgttgaag tacacttctc aaataaagag aagactgtca
     4201 tcaagggttt gaaagtagag cgtgttcaag caagtgctga agagaagaaa tactttgctg
     4261 gagaagatgc tcatgtcttt gaaatagaag gtttggatga aaaaggtcaa gatgttgatc
     4321 tctcttatgc ttctattgtg aaaatcccaa ttgaaaaaga taagaaagtt aagaaagtat
     4381 ttttcttacc tgaaggcaaa gaggcagtag aattggcttt tgaacaaacg gatagtcatg
     4441 ttatctttac agcacctcac tttactcatt atgcctttgt ttatgaatct gctgaaaaac
     4501 cacaacctgc taaaccagca ccacaaaaca cagtccttcc aaaacctact tatcaaccgg
     4561 cttctgatca acaaaaggct cctaaattgg aagttcaaga ggaaaaggtt gcctttcatc
     4621 gtcaagagca tgaaaatgct gagatgctag ttggggaaca acgagtcatc atacagggac
     4681 gagatggact gttaagacat gtctttgaag ttgatgaaaa cggtcagcgt cgtcttcgtt
     4741 caacagaagt catccaagaa gcgattccag aaattgttga aattggaaca aaagtaaaaa
     4801 cagtaccagc agtagtagct acacaggaaa aaccagctca aaatacagca gttaaatcag
     4861 aagaagcaag caaacaattg ccaaatacag gaacagctga tgctaatgaa gccctaatag
     4921 caggcttagc cagccttggt cttgctagtt tagccttgac cttgagacgg aaaagagaag
     4981 ataaagatta aatatcgaaa aatcttgtga aatctttcct tatatttcca aagtgtgata
     5041 taatagtttc gaataaaata aataaaggag tttttgtaac atggcaaaac ttactgttaa
     5101 agacgttgac ttgaaaggta aaaaagtcct cgttcgtgtt gacttcaacg taccattgaa
     5161 agatggcgta atcactaacg ataaccgtat cacagcagct cttccaacta ttaagtacat
     5221 catcgaacaa ggtggacgtg caattctttt ctctcacctt ggacgtgtga aagaagaagc
     5281 tgataaagct ggtaaatcac ttgctcctgt agcagctgac ttggcagcaa aacttggtca
     5341 agatgttgtt ttcccaggtg tcactcgtgg tgctgaattg gaagcggcaa tcaacgctct
     5401 tgaagatgga caagttctct tggttgaaaa cactcgttac gaagatgttg acggcaagaa
     5461 agaatctaaa aacgatcctg aacttggtaa atactgggca tcacttggag atggtatctt
     5521 cgtaaacgat gcattcggta cagctcaccg tgcacacgca tctaacgttg gtatctcagc
     5581 aaacgttgaa aaagcagttg ctggtttcct tcttgaaaac gaaattgcct acattcaaga
     5641 agcagttgaa actccagaac gtccattcgt ggctatcctt ggtggttcaa aagtttcaga
     5701 caagatcggt gttatcgaaa acttgcttga aaaagctgat aaagtcctta tcggtggtgg
     5761 gatgacttac acattctaca aagcacaagg tatcgaaatc ggtaactcac ttgtagaaga
     5821 agacaaattg gatgttgcga aagctcttct tgaaaaagca aatggtaaat tgatcttgcc
     5881 agttgactca aaagaagcta acgcatttgc tggttacact gaagtgcgtg acactgaagg
     5941 tgaagcagtt tctgaaggct tccttggtct tgacatcggt ccaaaatcta tcgccaaatt
     6001 tgacgaagct ttgactggtg ccaaaacagt cgtatggaac ggacctatgg gtgtatttga
     6061 aaacccagat ttccaagctg gtacaatcgg tgtgatggac gctatcgtga aacaaccagg
     6121 agttaaatca atcatcggtg gtggtgactc agctgccgca gcgattaacc ttggccgtgc
     6181 agacaagttc tcatggatta gtacgggtgg tggagcatca atggaacttc ttgaaggtaa
     6241 ggttcttcca ggacttgcag ccttgacaga aaaataagat tttataaata aatcaaagaa
     6301 gagagggatg aaagttcctc ttttcttttg cttaaaataa aaacgcttcc tctcaactat
     6361 tactcataaa aatcaccgat ttatgataaa atggaaatag aaagttgaga ttatgagtta
     6421 ttttaaaaaa tataaattcg ataaatccca gttcaaactt ggtatgcgaa cctttaaaac
     6481 aggtattgct gtttttctag ttctcttgat ttttggcttt tttggctgga aaggtcttca
     6541 aattggtgct ttgacagccg tttttagcct gagggagagt tttgatgaga gtgttcattt
     6601 tgggacttcg cgtattctag gaaatagtat cggtggactc tatgccttgg tcttcttctt
     6661 attaaatacc tttttccacg aagccttttg ggtgaccttg gtagttgttc caatctgcac
     6721 catgttaacc attatgacaa atgtagccat gaataacaaa gcaggggtta ttggtggtgt
     6781 agcagctatg ttaatcatta ccctatcaat tccaagtggt gagacaattt tgtacgtgtt
     6841 tgtgcgtgta ttagaaacgt ttatgggagt ttttgtcgca attatcgtaa attacgatat
     6901 tgatcgtatt cgtctctttt tagagaaaaa agaaaaataa tgttacattt tataacatta
     6961 tcaattgacg tttgtctttt tttagactat aatagacaga aagaaggaaa ttgtaaatga
     7021 aggaaaaaga atttcgccga aatatggctg tttttcctat cggcagtgtt atgaagttga
     7081 ccgatctatc ggcgcgtcag attcgttatt atgaagatca agagttgatc aagcccgatc
     7141 gaaacgaagg aaatcgtcgc atgtattcct tgaatgacat ggatcgtctg cttgaaatca
     7201 aagattatat ctctgaaggt tataatatcg ctgccattaa gaaaaaatat gctgaacgtg
     7261 aagcgaaatc caagaaagcg gttagtcaga ctgaagtacg tcgtgcactt cacaatgaac
     7321 tcctccaaca ggggcgtttt gcttcagtac agtcaccttt tggtcgcggt taggcaaccg
     7381 caagtagtca tagattaagg agaaaactta tgccaatcac agctgcagat attcgtcgtg
     7441 aagtcaagga aaaaaatgtt acctttattc gtcttatgtt ctcagatatt ttgggaacca
     7501 tgaaaaacgt cgaaattcct gctacagatg aacagttaga taaggtcttg tcgaacaagg
     7561 ttatgtttga tggatcttct attgaaggtt ttgtacgtat caatgagtcg gatatgtact
     7621 tgtacccgga cttggataca tggacagtct tcccttgggg agatgaaaat ggaagtgttg
     7681 caggtctgat ctgtgatgtc tatacaacag aaggtgaacc atttgcgggt gaccctcgtg
     7741 gtaatttgaa acgagctctt cgtcacatgg aagaagttgg attcaaatcc ttcaaccttg
     7801 gtccagagcc agaattcttc ctatttaagt tggatgaaaa tggggaccca acacttgaag
     7861 tgaatgacaa gggtggctac tttgatttgg cacctactga ccttgcggac aacacacgtc
     7921 gtgagattgt gaatgtcttg accaaaatgg gatttgaagt agaagcgagt caccacgagg
     7981 ttgcggttgg acagcatgag attgacttta agtacgatga agttctccgt gcttgtgata
     8041 agattcaaat ctttaagctt gttgttaaaa ccattgctcg caaacacgga ctttacgcaa
     8101 catttatggc gaagccaaaa tttggtattg ctggatcagg tatgcactgt aatatgtcct
     8161 tgtttgatgc agaaggaaat aacgccttct ttgatccaaa tgatccaaaa ggaatgcagt
     8221 tgtcagaaac agcttaccat ttcctaggcg gtttgatcaa gcatgcttac aactatactg
     8281 ccatcatgaa cccaacagtt aactcataca aacgtttggt tccaggttat gaagcgcctg
     8341 tttacattgc ttgggctggt cgtaaccgtt cgccacttgt gcgcgtacct gcttcacgtg
     8401 gtatgggaac tcgtcttgag ttgcgttcag tggatccaat ggcgaaccct tacgttgcta
     8461 tggctgttct tttggaagtt ggtttgtatg gtattgaaaa taaaatcgaa gcaccagctc
     8521 ctatcgaaga aaatatctac atcatgacag cagaagagcg caaggaagct ggtattacag
     8581 accttccatc aactcttcac aacgctttga aagctttgac agaagatgaa gtggttaaag
     8641 ctgctctcgg agatcacatc tatactagct tccttgaagc caaacgaatc gaatgggcaa
     8701 gttatgcaac cttcgtttca caatgggaaa ttgataatta tttagacctt tactaatact
     8761 aatatagaag aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata
     8821 cctataaccc ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac
     8881 tcttttgcga attagattta tcatcagacg tgtagaagaa aattaaggag tcactatgga
     8941 aaaattcaat ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta
     9001 caagtttact aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga
     9061 tatatttgaa gccatagtaa attgagacta gaatagtaca ctacatctgc taaaaatttc
     9121 tagaaactaa tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt
     9181 atctcaataa atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat
     9241 cgagcggaag aatatttctg ccgaggttgc caaggggaaa atcctgaaga tattgaattt
     9301 tatgatgaac aattacaggc agaaaaggtg gaggttttga atattcgatt ggaagtaaaa
     9361 aattgaatta tctacaatct catcaacaat aaacctattt caaccccatt atctataggc
     9421 gtatttttac gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg
     9481 atggaagtac tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga
     9541 ggttttctac attggttgaa attgtcagag gtggctctcc acgaccaatc aaagattatc
     9601 ttacttctga agtagatgga ataaattgga taaaaatagg tgatactgaa aagggtgaaa
     9661 agtatataaa taatgttaaa gaaaaaatca aaaaatcagg gcttaacaaa actagatttg
     9721 taaaaaaagg tacatttttg ttaactaatt ctatgagttt tggtagacct tatattttga
     9781 atgttgatgg tgcaatacac gatggatggt tggctatttc gaactatgaa aactcattaa
     9841 ataaagatta cctattctat attctttcat caaatgtagt ttattctcaa tttctatctc
     9901 taattagtgg agctgttgtg aaaaacttga atagtgataa agttgcttct attcttatcc
     9961 ctctcccccc actatccgaa caacaacgca taataagagc aatcgaatca gctttagaaa
    10021 aagtagatga atatgctgaa agttataata gactagaaca gctagataaa gaatttccag
    10081 ataaactaaa aaaatctatt cttcaatatg ctatgcaagg aaaattagtt gaacaagacc
    10141 caaatgatga atcagtcgaa gttttacttg aaaaaatacg agcagaaaaa caaaaactct
    10201 ttgaagaagg caagattaaa aagaaagatt tggacatttc tattgtttcc caag
//
LOCUS       DAVMHG010000065        10052 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMHG010000065 DAVMHG010000000
VERSION     DAVMHG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.065"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            241..588
                     /gene="ssrA"
                     /locus_tag="VYR08_001285"
     tmRNA           241..588
                     /gene="ssrA"
                     /locus_tag="VYR08_001285"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(771..858)
                     /locus_tag="VYR08_001286"
     tRNA            complement(771..858)
                     /locus_tag="VYR08_001286"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Ser,seq:gga)
     gene            1020..1760
                     /locus_tag="VYR08_001287"
     CDS             1020..1760
                     /locus_tag="VYR08_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3466808.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1757..2668
                     /gene="pfkB"
                     /locus_tag="VYR08_001288"
     CDS             1757..2668
                     /gene="pfkB"
                     /locus_tag="VYR08_001288"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV3466809.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLDIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALFVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2665..4617
                     /locus_tag="VYR08_001289"
     CDS             2665..4617
                     /locus_tag="VYR08_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3466810.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4781..7006
                     /locus_tag="VYR08_001290"
     CDS             4781..7006
                     /locus_tag="VYR08_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3466811.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7051..7479)
                     /locus_tag="VYR08_001291"
     CDS             complement(7051..7479)
                     /locus_tag="VYR08_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466812.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7617..8759
                     /locus_tag="VYR08_001292"
     CDS             7617..8759
                     /locus_tag="VYR08_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3466813.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGI
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8768..9982
                     /gene="thiI"
                     /locus_tag="VYR08_001293"
     CDS             8768..9982
                     /gene="thiI"
                     /locus_tag="VYR08_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV3466814.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         2884 a         1922 c         2302 g         2944 t
ORIGIN      
        1 tctcatctga aagtatttta gaataattct cttcgtgaat ttcttcaaaa cagatagctt
       61 catcttaggt atgtgttaat tggatttcgt aagctctatt tacaatcgta aagttatgca
      121 ctgagaaagt tgtagttagt cttccagttt gctaaatttc tcgaatagta tcttttttag
      181 gtatgtgatt tctttttgca tttttgagtt agataaggta taatggtttt attgtctttt
      241 ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt gtggcgacgt
      301 aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag ctgcctaaaa
      361 accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt attattagcg
      421 agatacgatt aagccttgtc tagcggtttg ataagagatt gatagactcg cagtttctag
      481 acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt gtagacaaat
      541 atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccattatt cctttgcatt
      601 cttttgcatt ccttggtaaa acgttgttaa atcaacgttt tttattttta tctttggtat
      661 tcatttgcat tcttttgcta aaaagggagt cacaaacaga ccctatttta aaaaaaggat
      721 agaaaaaagg atacaacatt tgtcgcatcc taaaaataat cttttttcga cggaagacat
      781 gggattcgaa cccacgcacg ctattacacg cctaccgcgt ttccaacacg gcctcttaag
      841 cctcttgagt aatcttccaa tacttactca aatagtctac cataaaggct cttatcttgc
      901 aataaaaatt ctagaaataa gaaaaatgat agattttgaa agaaaatgat aaaaaatgct
      961 tgacttcaaa agaaagtatg atagaatgaa tagtgtaaac gataacagga ggtgattcgg
     1021 tgttaaaaac agagcggaag caactgattt tagaggagtt aaatcaacat catgtagttt
     1081 ctctagaaaa attagttagt ttgctagaaa cgtcagaatc aacggttcga agagacttgg
     1141 atgagttgga agcggaaaac aagcttcgtc gtgtgcatgg tggagcagaa ctcccccact
     1201 ccttacagga agaagaaacc attcaagaaa aatctgtcaa aaaccttcaa gaaaagaagt
     1261 tgctggctca gaaagcagcc tctctcatta aagaaaaaga tgtcatcttt atcgatgctg
     1321 gaacaacaac tgcttttttg attcatgaat tggtcaataa gaatattaca gttgtgacca
     1381 actccattca ccatgccgct cagttggttg aaaagcagat tccaactgtc atggttggag
     1441 gaaacgtcaa gacggcgaca gatgctagta tcggtggcgt tgctcttaac cagattaacc
     1501 aattgcactt tgaccgtgcc tttatcggaa tgaatggtgt tgacgatggc tattatacga
     1561 ctcctgatat ggaggaggga gctgtgaaaa gagctatttt ggagaatgcc aagcagacct
     1621 acgtcttggt ggattcgtca aaaattggac aaacttgctt tgccaaggta gccccactca
     1681 aacgcgctat cgttatcact agtcaagggc atgagctctt gcaggttatt aaggagaaaa
     1741 cggaggtaat agaagtatga tttatacagt cacactcaat ccatccattg actatatcgt
     1801 tcgtttggac caagtcaaag ttggtagtgt aaatcgtatg gacagtgatg ataagtttgc
     1861 tggtgggaaa ggaatcaatg tcagccgtgt cttgaaacgc ttggatattc ctaatacagc
     1921 gacgggattt atcggtggct ttactggtaa atttatcaca gatactctag cagaggaaga
     1981 aatcgagaca cgttttgtcc aagtggcaga agatactcgt atcaatgtta aaatcaaagc
     2041 agaccaagaa acagaaatca acggaacggg tccaactgtt gaaccggttc agctagaaga
     2101 attgaaagct attttatcta gtctgacagc agaagataca gttgtctttg caggttcaag
     2161 tgctaaaaat ctaggcaatg ttatctataa ggatttgatt tccttgacgc gccagactgg
     2221 tgcgcaagtg gtctgtgact ttgaaggaca gaccttaatt gatagtttgg actaccagcc
     2281 tcttcttgta aaaccaaaca atcatgaact tggagcgatt tttggggtta aactcgaaag
     2341 tttagatgaa attgagaaat acgctcgtga gttactggct aagggtgctc aaaatgttat
     2401 tatctctatg gctggtgatg gtgccctttt tgtcacatct gagggagctt acttcgctaa
     2461 accaatcaaa ggaacagtca aaaattcagt tggagctggt gattctatgg ttgctggatt
     2521 cacaggtgaa tttgtcaaat caaaagacgt agtagaagcc ttcaaatggg gagtggcttg
     2581 cggaacggca actaccttct cagatgactt ggcaacggcg gaatttatta aagaaacata
     2641 tggaaaagtt gaggtagaaa aacgatgaaa attcaagacc tattgagaaa agatgtcatg
     2701 ttgctagatt tgcaggcaac tgaaaaaaca gctgtcatcg acgagatgat taaaaatttg
     2761 acagaccacg gttatgtaac agattttgaa acatttaaag aaggaatttt ggcgcgtgaa
     2821 gctttgactt ctactggttt gggtgatgga atcgcaatgc ctcacagcaa aaacgctgct
     2881 gtcaaagaag cgacagttct atttgctaag tcaaataagg gtgttgacta cgagagcttg
     2941 gatggacaag caactgacct cttcttcatg attgcagctc cagaaggtgc caatgatact
     3001 cacttggcag ccttggcaga attgtctcaa tacttgatga aagacggttt tgcagacaaa
     3061 cttcgtcaag caacatctgc agaccaagtt atcgaacttt ttgaccaagc ttcagaaaaa
     3121 actgaggaac ttgttcaagc acctgctaat gactctggtg actttatcgt agctgttaca
     3181 gcttgtacaa caggtattgc ccacacttac atggcccaag aagcccttca aaaagtagct
     3241 gctgaaatgg gggttggtat caaggtcgaa accaacggtg ctagcggtgt tggaaatcaa
     3301 ctaactgcag aagatatccg taaggctaaa gctattatca ttgcagcaga caaggccgtt
     3361 gaaatggatc gatttgatgg aaaaccattg atcaatcgtc cagttgctga cggtatccgt
     3421 aagacagaag agctaattaa cttggctctt tcaggagata ctgaagtcta ccgtgccgct
     3481 aatggtgcca aagctgcaac agcctctaac gaaaaacaaa gccttggtgg tgccttgtac
     3541 aaacacttga tgagtggtgt atctcaaatg ttaccattcg ttatcggtgg tggtatcatg
     3601 attgcccttg ccttcttgat tgacggtgct ttgggtgttc caaatgaaaa ccttggcaat
     3661 cttggttctt accatgagtt agcttctatg ttcatgaaaa ttggtggagc tgcctttggt
     3721 ttgatgcttc cagtctttgc gggttatgtt gcctactcta ttgctgaaaa accgggtttg
     3781 gtagcaggtt tcgtggctgg tgctattgcc aaagaaggtt ttgcctttgg taaaattcct
     3841 tatgccgcag gtggtgaagc aacttcaact cttgcaggtg tctcatctgg tttcctaggt
     3901 gcccttgttg gtggatttat cgcaggtgcc ttggttcttg ccatcaagaa atacgttaaa
     3961 gttcctcgtt cactcgaagg tgctaaatca atccttctat tgccacttct tggaacaatc
     4021 ttgacaggat ttgttatgct agctgtgaat atcccaatgg ctgcaatcaa cactgctatg
     4081 aatgacttcc taggcggtct tggaggaggt tcagctgtcc ttcttggtat cgtccttggt
     4141 ggaatgatgg ctgttgacat gggtggacca gttaataaag cagcttatgt ctttggtaca
     4201 ggtacgcttg cagcaactgt ttcttcaggt ggttctgtag ccatggcagc agttatggct
     4261 ggaggaatgg tgccaccact tgcaatcttt gtcgcaactc ttcttttcaa agataaattt
     4321 actaaggaag aacgtaactc tggtttgaca aacatcatca tgggcttgtc atttatcact
     4381 gagggagcga ttccatttgg tgccgctgac ccagctcgtg cgattccaag cttcatcctt
     4441 ggttcagcag tagcaggtgg actcgttggt cttactggta tcaaactcat ggcgccacac
     4501 ggaggaatct tcgttatcgc ccttacttca aatgctctcc tttacctcgt ttctgtcttg
     4561 gtaggagcaa tcgtaagtgg tgtggtttat ggttacctac gcaaaccaca agcataaaaa
     4621 atagaaaaat gaaaagattg gaccgtttgg tgcagtcttt ttctcttccc gaaatgcctg
     4681 tgaaatatgg tataatagaa gaatggcaaa caagaataca agtacaacaa gacggagacc
     4741 gtctaaagca gaactggaaa gaaaagaagc gattcaacga atgttgattt cgttaggaat
     4801 tgcgatttta ttgattttcg cagccttcaa attaggggct gcaggtataa ccctttataa
     4861 tttaattcgc ttgctagtgg gtagcctagc ttatctggcg atattcggcc tattaatcta
     4921 tctcttcttt ttcaagtgga tacgaaaaca ggaaggactc ttatctggct ttttcaccat
     4981 atttgctggc ttactcttga tttttgaggc ctacttggtt tggaaatatg gtttggacaa
     5041 gtccgttcta aaagggacca tggctcaggt tgtgacagat ctgactggtt ttcgaacgac
     5101 tagctttgct ggagggggct tgatcggggt cgctctttat attccaacag cctttctctt
     5161 ttcaaatatc ggaacttact ttattggttc tatcttgatt ttagtgggtt ctctcctagt
     5221 cagcccttgg tctgtttacg atattgctga atttttcagt agaggctttg ccaaatggtg
     5281 ggaagggcac gagcgtcgaa aagaggaacg ctttgtcaaa caagaagaaa aagctcgcca
     5341 aaaggctgag aaagaggcta gattagaaca agaagagact gaaaaagcct tactcgattt
     5401 gcctcctgtt gatatggaaa cgggtgaaat tctgacagag gaagctgttc aaaatcttcc
     5461 acctattcca gaagaaaagt gggtggaacc agaaatcatc ctgcctcaag ctgaacttaa
     5521 attccctgaa caggaagatg actcagatga ccaagatgtt caggtcgatt tttcagccaa
     5581 agaagccctt gaatacaaac ttccaagctt acaactcttt gcaccagata aaccaaaaga
     5641 tcagtctaaa gagaagaaaa ttgtcagaga aaatatcaaa atcttagaag caacctttgc
     5701 tagctttggt attaaggtaa cagttgaacg ggccgaaatt gggccatcag tgaccaagta
     5761 tgaagtcaag ccggctgttg gtgtaagggt caaccgcatt tccaatctat cagatgacct
     5821 cgctctagcc ttggctgcca aagatgtccg gattgaagca ccaatccctg ggaaatccct
     5881 aatcggaatt gaagtgccca actccgatat tgccactgta tctttccgag aactatggga
     5941 acaatcgcaa acgaaagcag aaaatttctt ggaaattcct ttagggaagg ctgttaatgg
     6001 aaccgcaaga gcttttgacc tttctaaaat gccccacttg ctagttgcag gttcaacggg
     6061 ttcagggaag tcagtagcag ttaacggcat tattgctagc attctcatga aggcgagacc
     6121 agatcaagtt aaatttatga tggtcgatcc caagatggtt gagttatctg tttacaatga
     6181 tattccccac ctcttgattc cagtcgtgac caatccacgc aaagccagca aggctctgca
     6241 aaaggttgtg gatgaaatgg aaaaccgtta tgaactcttt gccaaggtgg gagttcggaa
     6301 tattgcaggt tttaatgcca aggtagaaga gttcaattcc cagtctgagt acaagcaaat
     6361 tccgctacca ttcattgtcg tgattgtgga tgagttggct gacctcatga tggtggccag
     6421 caaggaagtg gaagatgcta tcatccgtct tgggcagaag gcgcgtgctg caggtatcca
     6481 catgattctt gcaactcagc gtccatctgt tgatgtcatc tctggtttga ttaaggccaa
     6541 tgttccatct cgtgtagcat ttgcggtttc atcaggaaca gactcccgta cgattttgga
     6601 tgaaaatgga gcagaaaaac ttcttggtcg aggagacatg ctctttaaac cgattgatga
     6661 aaatcatcca gttcgtctcc aaggatcctt tatctcggat gacgatgttg agcgcattgt
     6721 gaacttcatc aagactcagg cagatgcaga ctacgatgag agttttgatc caggtgaggt
     6781 ttctgaaaat gaaggagaat tttcggatgg agatgctggt ggtgatccgc tttttgaaga
     6841 agctaagtct ttggttatcg aaacacagaa agccagtgcg tctatgattc agcgtcgttt
     6901 atcagttgga tttaaccgtg cgacccgtct catggaagaa ctggagatag caggtgtcat
     6961 cggtccagct gaaggtacca aacctcgaaa agtgttacaa caataaaaaa atagcttctt
     7021 tccaagtttg gagggaagct attttagtgg ctattgattg cttttatttt ctgaagttgg
     7081 cgcattggac tgtttttcgt tttcagtagc aggtttactt gaagcaggag tagaagagtc
     7141 ctgagttgct gttttctgat cttctttttt ctcttccttg acgctagatt ttggtgtttc
     7201 ctcttgctgt gttttttctt gactagtgtt agtctcttta gttggactgg tgttttcctt
     7261 aggggattcc ttttggattt ctttgacaat ggttgtcgtc tggcttgtcg taggttcttt
     7321 tttaatattt ttgttattat ccaaggcgtt cataatcgtg atacttgcgg taatcaagcc
     7381 aaggatacta gcgattgtag caacggtttt ttgaaagact gtaaagcctt ttttgatgtg
     7441 ttctgttttt ggttttgaag ttctacgata gttagacata cattctctcc tttgattatg
     7501 atttattata ctctgtttct ttaaaaaaat cttaaaaaaa gagaaatttc cctttcttgt
     7561 cgcaagctcc gtttcatgat acaatagagg aagtgatttt agaaagcgtg agaaacatga
     7621 tttactttga taattcagcg acgaccaagc cttatcctga agcccttgaa acctatatgc
     7681 aggtcgcttc aaaaatttta ggaaacccat ctagtctcca tcgtttggga gaccaagcaa
     7741 cacgaatctt agatgcttct cgccaacaga ttgcagattt aatcggtaag aaaagcgatg
     7801 aaatcttctt tacctcgggt ggaacagaag gggataactg gcttatcaag ggtgtggcct
     7861 ttgaaaaagc tcagtttggc aagcacatca ttgtttcagc cattgaacat ccagcagtca
     7921 aagagtcagc cctctggttg aaaagtcaag gatttgaagt ggattttgct ccagttgata
     7981 agaaaggctt ggtcgatgtt gaggcgttag caggtttgat acggcatgat acaatcctcg
     8041 tttccatcat ggctgtgaac aatgaaatcg gctctatcca acctattgag gctatttcag
     8101 aattcttggc agacaagccg actatttcct tccacgttga tgcggttcag gcgcttgcca
     8161 aaattccgac tgaaaagtat ctgacagaac gggtggattg cgcgactttc tctagtcaca
     8221 agttccacgg ggttcgaggt gttggctttg tctatatcaa atctggcaag aagattacac
     8281 ctcttcttac aggtggtggc caggagcgag attatcgttc gacaactgaa aatgtggcag
     8341 ggattgcagc gacagccaag gccctccgtt tgtctatgga aaagctagat atctttagga
     8401 gcaagactgg gcagatgaag gcagtgattc gccaagctct tctgaactat ccggatattt
     8461 ttgtcttttc agatgaggaa aactttgcac ctcatattct gacttttgga atcaaaggtg
     8521 ttcgaggtga agtcatcgtt cacgcctttg aagactatga tattttcatc tcaacaacct
     8581 cagcttgttc atctaaggca ggaaaaccag ccggtatctt gattgccatg ggagtggaca
     8641 aagataaggc caagtcagct gtgcgtctta gcctagactt ggaaaatgat atgagtcagg
     8701 tcgagcagtt tttgaccaag ttaaaattga tttacaatca aactagaaaa gtaagatagg
     8761 agcattcatg cagtattcag aaattatgat tcgctacgga gagttgtcaa ccaagggtaa
     8821 aaaccgtatg cgtttcatca ataaacttcg taataatatt tcggacgttt tgtctatcta
     8881 tacccaagtt aaggtaacag cagatcgcga ccgtgcccac gcttacctca atggagctga
     8941 ttacacagca gttgcagaat ctctcaaaca agtttttgga attcaaaact tttctcctgt
     9001 ttataaggtt gaaaaatctg tagaagtttt gaagtcttct gtccaagaga ttatgcggga
     9061 catctacaag gaaggtatga cctttaagat ttctagcaag cgtagcgacc acaactttga
     9121 acttgatagt cgtgaactca accaaacact tggaggggct gtattcgaag ccattccaaa
     9181 tgtgcaagtt caaatgaaaa gtcctgacat caatcttcag gtggagattc gtgaagaagc
     9241 agcctatctt tcttatgaaa ccattcgtgg ggctggtggt ttgccagttg gaacttcagg
     9301 taaagggatg ctcatgttgt caggagggat tgactcacct gtagcaggtt atcttgctct
     9361 taagcgtggg gtggatatcg aggcagttca ctttgctagt ccaccatata ctagtcctgg
     9421 tgccctcaag aaagcgcagg acttgacccg taaattgacc aagtttggcg gaaatatcca
     9481 gtttatcgag gtgcctttca cagagattca agaggaaatc aaagccaaag cgccagaagc
     9541 ttatttgatg actctaactc gtcgctttat gatgcggatt actgaccgta ttcgtgaggt
     9601 acgaaatggt ttggttatca tcaatgggga aagtctaggt caagtagcca gccaaaccct
     9661 tgaaagtatg aaggctatca atgctgttac caacactccc atcattcgtc ctgtggttac
     9721 catggacaag ttggaaatca ttgacatcgc ccaggaaatc gatacctttg acatttcaat
     9781 ccaaccgttt gaagactgtt gtaccatttt tgcaccagat cgtccaaaaa caaatcctaa
     9841 aattaagaat gcggagcagt acgaagcgcg tatggatgtt gaaggcttgg ttgagcgagc
     9901 agtggctgga atcatgatta ctgaaatcac acctcaagcc gaaaaagatg aagttgatga
     9961 cttgattgac aatctgctct aattcagaaa atccaaaaga atagtgaaaa tcagtaaaaa
    10021 aagttagttt tttctctaaa aacaggtaaa aa
//
LOCUS       DAVMHG010000066        10014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMHG010000066 DAVMHG010000000
VERSION     DAVMHG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.066"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            95..358
                     /locus_tag="VYR08_001294"
     CDS             95..358
                     /locus_tag="VYR08_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466815.1"
                     /translation="MVFAFHLPPDELITNVIFKEKINSMLKCYIDRLLYVFINPTHFT
                     EKVNLQFYGSFFSYEFICREVGNILKNKGVKCNLNFFEGEEYL"
     gene            466..1467
                     /locus_tag="VYR08_001295"
     CDS             466..1467
                     /locus_tag="VYR08_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3466816.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            2074..2151
                     /locus_tag="VYR08_001296"
                     /pseudo
     CDS             2074..2151
                     /locus_tag="VYR08_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2653..4014
                     /gene="trpE"
                     /locus_tag="VYR08_001297"
     CDS             2653..4014
                     /gene="trpE"
                     /locus_tag="VYR08_001297"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV3466817.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETACFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFLGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSSLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATSDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            4011..4577
                     /locus_tag="VYR08_001298"
     CDS             4011..4577
                     /locus_tag="VYR08_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV3466818.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4588..5592
                     /gene="trpD"
                     /locus_tag="VYR08_001299"
     CDS             4588..5592
                     /gene="trpD"
                     /locus_tag="VYR08_001299"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV3466819.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5589..6356
                     /gene="trpC"
                     /locus_tag="VYR08_001300"
     CDS             5589..6356
                     /gene="trpC"
                     /locus_tag="VYR08_001300"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV3466820.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            6343..6942
                     /locus_tag="VYR08_001301"
     CDS             6343..6942
                     /locus_tag="VYR08_001301"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV3466821.1"
                     /translation="MTKVKICGLSTKEAAETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPVDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6920..8143
                     /gene="trpB"
                     /locus_tag="VYR08_001302"
     CDS             6920..8143
                     /gene="trpB"
                     /locus_tag="VYR08_001302"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV3466822.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAVGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            8136..8912
                     /locus_tag="VYR08_001303"
     CDS             8136..8912
                     /locus_tag="VYR08_001303"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV3466823.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKDLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8884..9543)
                     /locus_tag="VYR08_001304"
     CDS             complement(8884..9543)
                     /locus_tag="VYR08_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV3466824.1"
                     /translation="MIDIYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLH
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFQLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9613..>10014
                     /locus_tag="VYR08_001305"
     CDS             9613..>10014
                     /locus_tag="VYR08_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466825.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQM"
BASE COUNT         3065 a         1830 c         2366 g         2753 t
ORIGIN      
        1 taaaatcaat tttgatttag gtaaaataga atattttgat aataattgtt tgatacaagt
       61 atacaaattt atttcttttt atgatatttg tgaaatggtg tttgcctttc atttacctcc
      121 tgatgagttg ataacaaatg ttatctttaa agaaaaaatt aactctatgt taaaatgtta
      181 cattgacagg ctgttatatg tttttataaa tcctacacat tttactgaaa aagttaatct
      241 acaattttat ggtagctttt tctcatatga atttatttgt cgtgaagttg gaaacattct
      301 taaaaataaa ggtgtgaaat gcaatttaaa cttttttgaa ggggaagaat atttgtaaaa
      361 ttaattttgt gaatttgtgg atcttaaata atttactaaa ttctcttgtt aaaaaaaatt
      421 aaatattgta tagtaatatt agaatgttcg taatggagga ctgaaatgaa aaatataaat
      481 gggaaaaagg taactattta tgatattgca cgtttatcag gtttttctcc aaaaacagtt
      541 tcacgagtta ttaatggtgg agtaaatgtt aaagaagaaa cttatcaagc gattcaaaaa
      601 gttatagagg aattgtcgta tattccaaat gcgtatgcaa aaaatttaac taaaaaagaa
      661 gctataaata tattgatttc tgtaaaaaaa atcgactctt ttcctttaat ttggtttcat
      721 acgctgttag ataaagtgtt acggacgtgt aaagaatttg gtgttaatgc tatagttgag
      781 tactttggag aggaggatac aattagtaat tcaattatat caagcacagg tagcctagta
      841 gatggtgtta ttgtttttta tgaaagtgta gatgatatca gaattcagta tttaaagaag
      901 aaccatatgc cttttcttgt tttcggtgaa tctcaaacgt ctggagtagt ctatgtatct
      961 aataataatt ttcaagctac ttatgatatg atgaaagctg taactgaaga aaaatttaaa
     1021 aatatgtggt tgcttatggg aggagaatcc catgttaata aggatcgtga aagaggtgtg
     1081 cgttcttttc tgaatgataa aaattacttt atggatttaa aagtcattta tggcttatct
     1141 acaattgatt cagtttattc ttatgcaatg cagcacttga ctacccaaaa ttatccggac
     1201 attatttttg tttcaggaga tgaaaaggtt cagggcttga ttcgtgcgtg ctatgagaaa
     1261 ggaattctta ttccagatga tatttctatc attggatttg ataatattcc tatttctcag
     1321 tattatactc cagctttgtc tacaattgct cccaattatg ttaaattggc taaagaaatg
     1381 atagaaggtg ttttagcaat tattaaaggg gaaagtgtca catctgttga agtttctcct
     1441 aaatttgtta ggagacagag tttctagtat tgtttaattc ttgaaaatcc tagtggaaaa
     1501 gatggagtgt tctaaaataa gttctgttta gcctccattc agtttctatt gagttggatc
     1561 caatttctaa caagtgggac atgttcatgt tcgatctaag gaagacatgg tcacattcga
     1621 atgatatttt caaaaaaatc gtaaaaaagt cttgacaaag aaaaaacaaa gtattaaaat
     1681 aagttcaaca aatcagaaaa gtaactattt ttgatcttca gggagcctgt ggtgattgtg
     1741 aataggtggt tggaagtagt gaaagtgggc tgattttgaa aatgaatttg aaacaatgaa
     1801 aattcggtgc gcacacctta cagtgcagct tgttgttaaa caaggcagag atgtaaaggg
     1861 atagtccctt tataattgag gtggcaccgc gttaccaacg ccctcacacg gaagtatatt
     1921 ctgtgtgtgg gcttttttct atccgtcgtt tggtttatct tttattaggg ccttaagtcg
     1981 ctttgatgaa cttgagttct atctacagat ccttgcctac tactaaaagc aaacaaaaag
     2041 ctggactaat acgaaaaaag ctagtgatac tcaatgaaaa tcaaagaaca aactaggaaa
     2101 ctagccgcag gttgctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc
     2161 tcaaaacact gttttgaggt tgcagataga actgacgaag tcagtaacca atacctacgg
     2221 caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataaaggtg taccgagatg
     2281 aacatctctt catcaatgtc tctggtcgtg gaggcaagga cctagctgcg attgcagatt
     2341 actagaagct aaaaaataat agatggaaaa attacagttc tttctctcac agagagcttg
     2401 tggttgctga aaacaagcag agaaagctgt aaagttgggt tcatctgaaa cgagagaaga
     2461 aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag atatctgaga
     2521 caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt tcgtcctcac
     2581 gcaagttatt ttgcgtgggg atttttcata cagtcaccac ggactagaag tagaactgaa
     2641 agggaaatta caatggaacg aatcattcat ggagatgtct tatcaccaat cttggcttat
     2701 atgcgcttaa aggggcaaca taaggttatt ttagagagta ttccgagaga caaggaaaca
     2761 gcttgttttt ctatcctagc ctataatcca gtttttgaga ttaagtttga aaatggggtt
     2821 ctctatcaaa atggtcaagt gattgatcgg gatcccttgg atttccttta tgaagtgatt
     2881 cataagagtc agcaccactc agagctccct tttggtggtg gagccattgg ttttttgggt
     2941 tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac aattgggacg
     3001 ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa gaaggagaag
     3061 atacatgtca tagaggatgc actttatagt gagcgcagtc aagaggcctt ggaaaaatcc
     3121 ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga agacttggat
     3181 ctatcttcgt tagacttcaa accgcatatc gctccacata agtttgaggg aatggtagaa
     3241 acagctcgtg acttgattcg taacggcgat atgttccaat gtgtactcag ccagcgtttc
     3301 tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt gactaatcct
     3361 tctaattacc tttatttcta tgattttggg gattatcaaa tcatcggagc tagtccagaa
     3421 agtttagttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg gacgcgacca
     3481 agaggggcta cggatgaaga agacaagacc ttggcgactg acctcctctc tgatgagaag
     3541 gaaacagcag aacatcgaat gttggtagac ttggggcgta acgatattgg ccgcatctct
     3601 gaaacggcca gtgtccaagt tactaagtat atgaaggtgg agctcttccg ctacgtcatg
     3661 catttgacca gcgtggtcaa ggggcgtttg cttccagaac tcacggccat ggatgccttg
     3721 aaagctacac ttcctgctgg aaccgtttct ggagcaccaa agattcgggc gatgagacgc
     3781 atctatgaac tggaaacgga aaaacggggc gtatatgcag gagcaatcgg ctacttgtct
     3841 gcgacgagtg atatggattt ggccattgcc atccgaacca tgattctcaa aaatcaaaga
     3901 gcctatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa cgaataccaa
     3961 gaaaccatta acaaagcaaa atctatgact agaattggag aactaagacc atgattttat
     4021 tgattgacaa ctatgattct tttacctata acttggccca atacattggg aattttgcag
     4081 aagttcaggt tctgagaaat gatgattcca agctgtatga agaagctgaa aaagcagatg
     4141 gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg gaagacatga
     4201 ttcgtgattt tgcaggcaag aagccgattc ttgggatttg tttgggccac caagccattg
     4261 cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg aaacagagca
     4321 atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggaagga cgtgcggtca
     4381 tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg acagctcgtt
     4441 cgactgatga ccaagccatc atggggattc aacataaaaa cctaccgatt tatggcttcc
     4501 agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg aattttatcg
     4561 agaaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact agcaaaattt
     4621 gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac tgggcgtgta
     4681 actgaagcgc agattgcttc tctcctctta gctcttaaga tgaaggggga aacacctgaa
     4741 gaacgcacag ccattgccca agtcatgaga ggacatgccc agcatattcc aactgagatt
     4801 catgatgcca tggacaactg tggtacaggt ggggacaagt ctttcagttt taatatttcc
     4861 acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaagca tggtaaccgc
     4921 tcgatttctt ctaaatctgg ttccgcagat gtcctcgaag ccttgggaat caatcttgac
     4981 ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt tctcttcgct
     5041 aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt gggaattcca
     5101 acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac acagcttctt
     5161 ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa tatgggtcgc
     5221 aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt gaacggaaca
     5281 accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac tccagaggat
     5341 ttgggaatgg aaggctatgc tatggaagat attcgtggtg ggaatgctca ggaaaatgca
     5401 gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac gacagtcttg
     5461 aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga aggagttgcc
     5521 ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact gttacaggag
     5581 taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggcg cgtgaggtgg
     5641 agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg gcagaatttt
     5701 tgaagaatca tcaggaccgc ttgcaggtaa tcgctgaggt caagaaagct agccctagtt
     5761 tgggagatat caatctcgat gtggatattg tgcaacaggc ccagacttat gaagaaaacg
     5821 gagcagtgat gatttcggtg ttgacagatg aggttttctt taaagggcat ttggattatc
     5881 tacgggaaat ttccagtcag gtagagattc cgacgctcaa caaagacttt atcatagatg
     5941 aaaagcaaat catccgcgct cgcaatgcag gtgcgacagt tatcttgctc attgtggcag
     6001 ccttgtccga agaacgcctc aaggaactgt atgactacgc gacagagctt ggtctggaag
     6061 tcttagtgga gactcacaat ctagctgaac tagaggtagc ccacagactt ggtgctgaga
     6121 ttatcggggt caacaaccgc aacttgacta cctttgaggt cgacttgcag accagtgtag
     6181 atttagcccc ttactttgag gaaggtcgct attacatttc tgaatctgcc attttcacag
     6241 ggcaggatgc ggaacgacta gccccatact ttaacggaat tttggtaggg acagctctta
     6301 tgcaggcaga aaatgtggcc cagagaatca aggagttgca gattgacaaa ggttaaaatt
     6361 tgtggactat cgaccaaaga agcggcggaa acagccgttt cagcaggagc cgactatatc
     6421 ggttttgtct ttgcacctag taaaagacag gtgactttag aagaggcagc tgagttggca
     6481 aagcttattc ctgtagatgt gaaaaaggtt ggagtatttg tttcaccaag tcgggtagaa
     6541 ctgctggaag cgattgacaa agttggcttg gacttggttc aagttcacgg tcaggtggca
     6601 gatgatttat ttgaaaattt gccttgtgcc agtattcagg ctgtgcaggt agatggaaat
     6661 gggcatgtcc ctaattctca ggcagattat ctactctttg atgcccctgt ggcaggaagt
     6721 ggccagccct ttgattgggg tcaactggat acgactggac tagcacagcc cttctttatc
     6781 gcaggtggcc ttaatgaaga taatgtagta aaagcaattc aacattttac tccctatgca
     6841 gtagatgtat cgagcggagt ggagacagat ggacaaaaag atcatgaaaa gattagaaga
     6901 tttatagaga gggtaaagca tggcatatca ggaaccaaat aaagatggat tttacggaaa
     6961 attcggcgga cgttttgtcc cagaaacatt gatgacagca gttttggagt tggagaaggc
     7021 ctaccgtgaa agtcaggcag acccaagttt ccaagaggaa ttaaaccagc ttttgcgcca
     7081 gtacgtagga cgtgaaactc ctctttatta cgcaaaaaac ttgacccagc atatcggcgg
     7141 agccaagatt tatctcaaac gggaagacct taaccataca ggagcccaca agattaacaa
     7201 tgccttagga caagtttggc ttgccaaacg catgggtaaa aagaaaatta tcgcagaaac
     7261 gggtgctggt cagcacggtg tggcaactgc aactgctgca gccctcttta acatggaatg
     7321 taccatctac atgggtgagg aagatgtcaa acgccaagcc ctcaatgtct tccgcatgga
     7381 gcttttggga gccaaggtcg aggccgtgac agatggttcg cgcgtgctca aggatgcggt
     7441 caatgcagcc cttcgttcat gggtggctaa tatcgatgat acccactata tccttggttc
     7501 tgccttgggt cctcatccat tcccagaaat cgttcgtgac ttccaaagtg ttattggtcg
     7561 agaagctaaa caacagtacc gtgacttgac aggtcgagat ctgccagatg ccctagtagc
     7621 ctgtgttggt ggtgggtcga atgctgtcgg gctcttccat ccctttgtag aagatgagtc
     7681 agtagctatg tatggagctg aagcagcagg gcttggtgtg gatacagagc accacgcagc
     7741 taccttgacc aagggtcgtc caggtgtcct tcacggttcc cttatggatg tgctccaaga
     7801 tgcccatggt caaattcttg aagccttctc tatctcagca ggtttggact atcctggtat
     7861 cggtccagag cattctcact accacgatat taaacgtgcc agctatgtcc ctgtgacaga
     7921 cgaagaagcc ttggaaggat ttcaactctt gtctcgtgtg gaagggatta tcccagcttt
     7981 ggaatctagc catgctatcg cttttgcggt gaaattggcc aaagaacttg gaccagaaaa
     8041 gtctatgatt gtctgcctat caggtcgtgg ggacaaggat gtggttcaag tcaaagaccg
     8101 cttggaagca gatgcagcaa agaagggaga agctcatgcc taagacacta acagaaaaat
     8161 tgaatgctat aaaagtaact ggaaaaggaa ttttcgttcc ttatatcatg gctggtgacc
     8221 acgaaaaaga tttggacggt cttgctgaaa caattcactt tttagaagat ttgggtgtct
     8281 ctgcaattga agtgggtatt cccttttcag accctgttgc agatggacct gttatcgaag
     8341 aagctggctt gcgcagttta gcccacggga cctctaccca ggctttagtt gaaaccttga
     8401 aaaccattga aacagagatt ccactggtca tcatgaccta cttcaacccc ctctttcagt
     8461 acggtgtgga gaactttgtc aaagatctgg ctgatacagc tgtgaagggc ttgattatcc
     8521 cagacctgcc tcatgaacat gccaactttg tagaaccatt tttggcagat acagatatcg
     8581 ccttgattcc tttagtaagc ttgaccacag gaattgagcg ccagaaagag ttgattgaag
     8641 gggcggaggg attcgtctat gccgttgcca tcaatggggt gacagggaaa tcaggcaatt
     8701 accgtgcaga tttggacaag cacttggcgc aacttcatca agtggccgac atcccagtct
     8761 tgacaggttt tggtgtatct agtcaagccg atctggaacg cttcaatgcg gtgtcagatg
     8821 gtgttatcgt cggttcaaaa atagtaaaag ctctccacca aggagagccg attcaggatt
     8881 ttatcagaca agcagtagct taccaaaaat aatcaaacaa gtagcaagta agaggaaagg
     8941 cacgaaagga agtctttcct ttttcttttg caggagaaag gccaggatac ccgtcgcaga
     9001 agcgaactga atcaagatca gtaactccgt tacgctaaag acgagagcac aagaagctaa
     9061 aaagaggaaa tcccctgcac ccatgcggat atcgataaaa tgagccaaaa ttccaagtat
     9121 gaggaaggag accatgacca gattccagcc agaggaagct attaggatta gctggaaagt
     9181 catccagacc agtaagggat attcctgatg gtgaaagtcg tagataccca aggtcaaacc
     9241 agcggtgatt aggacgactt gccccaagga aagcaattcc caagagtaaa gcagaaagag
     9301 gagtcctaag cctagttcaa agagggcata ccagacagga tagcgaactt tgcagtagcg
     9361 acagcgaaag cgattgaaga cctgtgagag aatcggaatc aaatctaagg gatgcaagcg
     9421 agtctgacag gaatcgcagt gactggctga actgataatg gattgctctg gaaaacggtc
     9481 aatgaccaaa ccaagaaagg aagcgagaat gctcccgaca agaaaaaaat aaatatcaat
     9541 catacttatc tattcgtaaa aaatgggaga agtagtataa tggagaaaca tagataagat
     9601 ggggaggaaa agatgacaat tcgttttgaa gaaaaggtga gtatagaaaa cgctcagctc
     9661 gtatgccagt ggtccaactc cttaggcaaa gtctttcaag aacaatggat gggaccaagg
     9721 attccttttc cactgaccct tcaagccttg gaaggagtct tttcaatctt tgatgaacaa
     9781 gagtttgtgg gacttatcca gaaaatcagg ctagaagaca gcaatcttca tatcgggaga
     9841 ttttttatca acccccagaa acaggggcaa ggcttagata gccaagcttt aaggaaattt
     9901 gttagtttgg cctttgaaaa tgaagatata gatagtattt ctctaaatgt cttcgaggca
     9961 aatcaaagag ttcagaatct ttaccaaaaa gaaggatttg aaatcgttca aatg
//
LOCUS       DAVMHG010000067         9824 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMHG010000067 DAVMHG010000000
VERSION     DAVMHG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.067"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(246..878)
                     /locus_tag="VYR08_001306"
     CDS             complement(246..878)
                     /locus_tag="VYR08_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466826.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(880..2895)
                     /locus_tag="VYR08_001307"
     CDS             complement(880..2895)
                     /locus_tag="VYR08_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV3466827.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWFIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGEGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEVGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3241..4104
                     /locus_tag="VYR08_001308"
     CDS             3241..4104
                     /locus_tag="VYR08_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3466828.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            4487..5107
                     /locus_tag="VYR08_001309"
     CDS             4487..5107
                     /locus_tag="VYR08_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466829.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            5193..5636
                     /gene="tsaE"
                     /locus_tag="VYR08_001310"
     CDS             5193..5636
                     /gene="tsaE"
                     /locus_tag="VYR08_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV3466830.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLSEELQYGV"
     gene            5626..6144
                     /locus_tag="VYR08_001311"
     CDS             5626..6144
                     /locus_tag="VYR08_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466831.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            6152..7168
                     /locus_tag="VYR08_001312"
     CDS             6152..7168
                     /locus_tag="VYR08_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV3466832.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            7253..8509
                     /locus_tag="VYR08_001313"
     CDS             7253..8509
                     /locus_tag="VYR08_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV3466833.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKTSSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            8564..9730
                     /gene="recA"
                     /locus_tag="VYR08_001314"
     CDS             8564..9730
                     /gene="recA"
                     /locus_tag="VYR08_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV3466834.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         3094 a         1727 c         2054 g         2949 t
ORIGIN      
        1 ttgcaaaata tcatctacca aattttcaat tattaagatt ttttgcagtt tatctgaatt
       61 agaatcgcaa ggcttctcaa taatataatt gtcgaatttt ttcattctaa cttcctctcc
      121 actatttcct cctttatcaa gaaaaagtta cctcaatatt aataaagtac aattctaaat
      181 taactccaat tattgattca tgataatatt tcagtaactc atcgtgcaat taaaaacaag
      241 gatttttatt tcagatgatc catcgtgaac acttcatcag ccatctccca aattgccgga
      301 ttatgtgttg cgataatgat tagcctatta tcatctcgaa gagatagcaa aatctccata
      361 atcaactgag aggttgctgg gtctattgaa gctgttggct catctgccag aataaagggt
      421 ggattcttta agataatttt tgccaaggca actcgttgcg attctccgcc cgataactca
      481 aagatgcgct tatctaggtc aagataagcc aggccgacct gttctaaagc ctgcttctgc
      541 ctcaaccgct gttccgaccg actcaacttt tgaccaatga gacctagctt aaggttttct
      601 tcaatacttt ggttttcaat taagccaaag ttctggaaga ggtagcccaa ttcgtgacgg
      661 aaaaaatcac tcgatttata attggccaag tctttacctc ggtaaaaaat cgtcccatca
      721 taaggttcta atttcccaat catgttcatc aaggttgttt ttccgctacc acttgaacca
      781 attaaggcat agacttttcc agcctcaaat gtcattgaaa gattcgaaaa taactctcgt
      841 tctccaaaag atttactcac ctgtttaagt tcaatcatat taacctccct tcaaaacaag
      901 catggacatc ttgttttctt tctgcatttg gacatgtaac tgtaaaagag atagaccagt
      961 aaagagtaac aagactaaga aagcaactcc tacctctacc tgaagaaata cactcgcaac
     1021 aaatcccagt aaaaacacac ccagttgagc aaagagataa gtgcgatgga tttctaagaa
     1081 cctgagacct gcaatgcgtt tgataaaaat ggcacgtcta aattcttcaa agtagagcct
     1141 attcatagtg ttaaacaaca agattgaagt tgcaatccca agcacagctc ctgctagcat
     1201 tacccaacgt tccctctgga tattatccaa taatgtgatg tagttgtggt aacctgtttg
     1261 catttctgag acccaatttt caatgccttg tctctggata agctcctggg catcagacaa
     1321 ttgattaaag agaacgtagt tctgtactgc gtctatccaa aacaaaatgg actgtggacc
     1381 agttgattgg ggtgttataa caatgatgat tggatctttc aaaaactgct ggtaagaaat
     1441 aggggtcgta ttatacacaa aacgatcctg acctgattct aaataaccta ccgtagcagt
     1501 catttgctgt cgttcatctt gactagacat gcgactggtt aagtcgtctt caaaaacaga
     1561 tttataatgt tcttcctctg aacggaggtg ttcaggcagc aataagacaa actcccctac
     1621 attaaggtga ttcatctttt gctcaatagt tgtatctaca ggaatgtttt gacgttccaa
     1681 gtattgcggt gtgacgataa ggacattgcc attcgggttg taatcgtgcc attctgtaga
     1741 ggttatcaag tttttggagg aagccatgcc attttgcaaa gtacggtcaa ttaactggtg
     1801 tctagataag aaagcctttt gttctgaaac agccagatcc atcaattgat accaagttct
     1861 gagtttcctt tgagcttgtt catcaaaacc aggactcgtt ccttcacggc tgattgatag
     1921 ggtaatcagt tgtctttcct gactccaagc ctcttgtcca atccgatgct gttgccaggc
     1981 ttgggaatac tttaagctac tccctattcc cagcgttaca ataataatcg ctaatagttg
     2041 accgattaaa atcaaactaa tgattcctct gacaggcact tgccctttta taatctgcat
     2101 caggtgtatc ttttttatcc caactgcgaa gagttgagcg aaaaacaggg agatcgacaa
     2161 caagatgaga ttataactga gcaagccttc tcctatggtc attagggatt gcgttggaag
     2221 cgacagaatt ttttgcataa gaatggcgag cacgccagct aaactgaaac caacagctat
     2281 cgctttcaaa tcctcaccaa ctggtctaag gaaaatgaac caccgtttct ctcctgaaat
     2341 gaggcgaatg cccgatgacc gtaattggct aatttgacta attaaagtca gtgctacaaa
     2401 ggttaagatg aaaattaata gactgattaa ctgaaatccg ttactaaaga tagccattaa
     2461 cgtagatagt ttagatggaa ttgtcaggtg catgttagtc aagccaagtt gacttagctc
     2521 ttcccttagc aagtgaatat ctaggtgtcc atcgaataca aaatagtttg tttcaacact
     2581 actactttga gcatcttcta gatttttttc ctgaagccca tcaggcaact ttccgtcccc
     2641 aaaggtcgta taagaaaaaa ctgtagtacc ttctgagtta ggatcttggt gttgaatcgc
     2701 aataaagcta tctgtttctt ctgctagttt ttccaagcgt gtagcaacat gctgaaaagt
     2761 tgtttcaggg gaagaatcac gtacaacaac gttggggtaa taatgggaga catatgtatc
     2821 agtccaaatg gtaaataccc aaacaaagaa caaactagca agcaggttgg atatgagtat
     2881 aaataatttt ttcatagtgc attccttatt ctaaaacaga gggcagaaat acccctgccc
     2941 tctataaact aacaagctta ctaaactatt gataaaagaa tcttcttcaa ttattcttta
     3001 ccatcccgca tcaaatgtaa ctaatttttt aattccacgt ttttttctca tgcaatgaaa
     3061 cccctttctt tttccttgtt tttattttaa tatatatata ttattcggtc aagcattttt
     3121 atgtaatttt tgactttttt tagtttatta ccataaagaa ttatttattt ctttaacttg
     3181 ctattttgaa ctaaaaattt tataatgcaa ttaagcaaat aggaaggttt attatcttta
     3241 atgagtacac tcgcagagaa attcagattg aaaagaaaag agttgagact ctcccaacaa
     3301 actcttgcag aaggaatttg tgaacaaagc cagattagta aaattgagag agggcatttc
     3361 attccctccg cagacctttt gttcaaactc tctcaacgac ttgaagtacc attagattat
     3421 ttttttaatg aacaaattga aattaaatct aacctctcta atttcaagca attgtctgct
     3481 cgactattag acgatagaaa ttatgacgat ttggaatata tttatagaat agagattgaa
     3541 cgaagtactt ttctaacact agaagaccga acttaccttg aatggattaa agctattatt
     3601 gacttctatc aatatgacag taagtgtgag gcaatttctt cattggaaaa tatattatta
     3661 aaagtctctt caaatactct gatttattta aaggcgttga atactctatc taatttctat
     3721 tccttagtgg gtcgtgaaca agaatatgag gcaaactact ctcatttaat agagttgtat
     3781 cagacaaaaa atttggacca tcaagagttt ttatttggct acatcagagt tcgttacaac
     3841 tacgctcact acctagtatc aaaggaaaaa tataatgaag ccatccaaga agctcttgaa
     3901 acgattgaac tctgtaaaca aagacagaca agctaccaat tggctcctct acttattctt
     3961 gtaggaaatg ctggagccaa atttctagac agagaacaag tcaaaaatta ttatatagaa
     4021 gcaagagagt tatgtaagat ttataacaat cctttaatgt tgatgaagat agaaaattat
     4081 ttgaaggaac tagatactgt ttagttaatc ttgacattat agtttttctg aaacgttaaa
     4141 taacctgtaa ggctgatagt tagattaata ctatcagcct tataattatg tgactctgag
     4201 tccgtctcgc tccgtgaggt gcgagacaaa acaaccaccc gtttgacgga ttttcatgat
     4261 ttcaaattta tattcttcga aaatctcttc aaatcacgtc agctctatct gcaatctcaa
     4321 aacagtgttt tgagcaacct gtggctagct acctagtttg atctttgatt ttcattgagt
     4381 attagtctct agtgtttgtt tagcgtctta aagagacatt tgagaagatt agaaacagtt
     4441 ctctcttttc tcctagtata atgattggta ggaagggaga gagaagatga aatcaatgag
     4501 aatcttattt ttgttagctt taattcaaat cagtttgagt agctgtttct tatggaagga
     4561 atgcatcttg tcctttaaac aaagtacagc ttttttcatc ggaagcatgg ttttcgtttc
     4621 aggaatctgt gctggagtaa attatcttta tacccgtaag caagaagtcc atagtgtcct
     4681 agccagtaag aagtcggtga agctttttta cagtatgtta ctcttaatta atttgttagg
     4741 agctgttctt gttttgtcag ataacttgtt catcaaaaat acgctgcagc aagaattagt
     4801 tgacttttta ttgccatcct tctttttcct atttgggcta gatttgctga tttttttacc
     4861 cttgaaaaaa tacgtgcgcg attttcttgc tatgctagac agaaaaaaga cagtgttggt
     4921 gactatttta gcaacacttc ttttcttaag aaatccaatg accattgtct cacttctgat
     4981 ttatattgga ctgggcttgt tttttgcagc ctatcttgtc ccaaattcgg ttaagaagga
     5041 agtttccttt tatggtcata ttttccgaga tcttgtattg gtcattgtta cgctcatttt
     5101 cttttagaga aagcctgttt tttatggatg ttatgatgat aagtttagta ggaaaataga
     5161 cacaaaagaa aagtttttgg tataataaga gtatgtacat aaaaaatgaa gaagagttgc
     5221 aagccttagg ggagcgtttg ggccatctat tagcaaagaa tgatgtttta atcttaattg
     5281 gagaactggg tgcaggtaaa acgaccttta ctaaaggact tgcaaaagga ttacagattt
     5341 ctcaaatgat taaaagtcct acctatacta tcgtgagaga gtatgaaggt cgacttccac
     5401 tttatcacct agatgtttat cgtattgaag gagatgctga ttctatcgac ttggatgagt
     5461 ttatctttgg tggcggcgtg actgttattg agtggggaaa tctcttagga gatgccttgc
     5521 cagatgctta tttagaattg gaaattctaa aagaagcaga tggacgccgt ttaaattttc
     5581 aggcaaaggg tttgcgtgct gagaaattgt cagaggagct tcaatatgga gtatgaattg
     5641 ctcattaggg aagcagagcc caaagatgca gctgaattag tggccttttt aaatcgtgtg
     5701 agtttggaga cagactttac cagcctagac ggagatggta ttctcttgac tagtgaggag
     5761 atggaaatat tcctcaacaa gcaagctagt tcggacaatc agataacctt acttgcattc
     5821 ttaaatggca aaattgctgg tattgtaaat attacagctg atcagcgtaa gagagtccgt
     5881 catattggag atctcttcat tgtgattgga aaaagatatt ggaataatgg cttgggaagt
     5941 ttgttgctag aagaagcgat agagtgggca caagcaagtg gcattctgcg tcgtctccaa
     6001 ctgactgtcc aaactcgtaa tcaagcagca gtccatcttt atcaaaagca tggctttgtc
     6061 attgaaggta gccaagagcg tggggcatat atagaagaag ggaaatttat cgatgtttac
     6121 ttgatgggta aactgatagg ttagtagaaa tatggttaaa aaaattattg gaatggtgct
     6181 agctttactt tctgtaactg tagtaggagt aggtgttttt gcttatacta tttatcaaca
     6241 agggacagaa accttagcta aaacctataa aaaaatcggc gaagaaacca aggttattga
     6301 agcgactgaa cctctaacca ttctgttaat gggagtggac accggaaatg ttgaacgaac
     6361 tgaaacttgg gtcggtagaa gtgatagcat gatcttgatg acagtgaatc ctaaaacgaa
     6421 aaaaacaaca atgatgagtt tagagcggga tattctgacg cgcattgaat cagggaatgg
     6481 tcaggctcat gaagcgaaac tgaactcagc atatgcagat ggtggagcag agcttgctat
     6541 agaaaccatt caaaaaatga tgaatatcca tattgatcgc tatgtgatgg tcaatatgag
     6601 agggttgcaa aaattagtgg atgcagtagg aggtattaca gtcaataata tcctaggttt
     6661 cccaatttct atcagtgacc aagaagaatt taataccatt tctatcggtg ttggggagca
     6721 acatattggg ggagaagaag ccctagtcta tgcacgaatg cgttaccaag atcctgaggg
     6781 ggattatggt cgtcaaaaac gtcaacgtga agttattcaa aaagtcatgg aaaaagctct
     6841 cagtttaaat agcattggtc attatcaaga gattctaaaa gctttgagtg acaatatgca
     6901 gaccaatatt gatttgtctg caaaaagtat ccctaacttg ctaggctata aagattcatt
     6961 taaaaccatt gaaactcagc agttgcaggg tgaaggagag atacttcaag gtgtttctta
     7021 ccagattgtt tcgagagcac atatgttgga aatgcaaaat atactccgac gttctttggg
     7081 acaagaagaa gttactcagc ttgaaaccaa tgcggtttta tttgaagatt tatttggcag
     7141 agcacctgtt ggtgatgaag ataattaact tttgatacaa ataaaaaaat caatcgtagg
     7201 aaaattcctg cgattttttc aaaaaaatac gaatagatag gtaggaggaa acatgaaagc
     7261 agaaatcatt gctgttggaa cagagatttt gacaggacag attgtcaaca ccaatgccca
     7321 gtttttgtcg gaaaaactag ctgagattgg ggtagatgta tattttcaga cggctgtagg
     7381 agacaatgaa gttcgtctct tgtctttgct tgagattgcc agtcaacgta gcagtctggt
     7441 gattttgaca ggcggtttgg ggccaactga ggacgaccta accaaacaaa ccctagctaa
     7501 atttttaggg aaagcattag tctttgatcc tcaggctcag gagaagttgg atatcttttt
     7561 tgccctgcga ccagactatg cccgaacacc gaataacgaa agacaagctc aaattgtaga
     7621 aggagcgatt ccactgccaa acgaaacagg actggctgtt ggaggaaaat tagaagtaga
     7681 cggagtgacc tatgtcgtcc ttccaggtcc gccaagtgaa ttgaaaccca tggtcttaaa
     7741 ccaacttcta cccaagttga tgacagggag caagctgtat tcccgagttc ttcgtttctt
     7801 tgggattggc gagagccagc tggttacgat tttggctgat ttaattgata atcagatcga
     7861 tcctaccttg gccccttatg ccaagacagg agaagtcact ctacgtctgt caacaaagac
     7921 tagcagtcaa gaagaggcga atcaagcgct ggatatcttg gaaaatcaaa tcttggactg
     7981 ccagactttc gaaggaattt ctttacgaga cttttgctat ggttatgggg aagaaactag
     8041 tttagccagc attgtggtag aagaactgaa aaggcaaggg aaaaccatcg cggctgcaga
     8101 gagtttgacg gcaggtcttt tccaagctac cgtggcgaat ttttctggag tttcaagtat
     8161 atttaagggt ggttttgtga cctatagctt ggaggaaaaa tcaaggatgt tggatattcc
     8221 tgccaagaat ttggaagaac atggtgtggt gtctgaattt acagctcaga agatggctga
     8281 gcaggcacga agcaagaccc agtctgattt tggaattagt ttgactggag tggcaggacc
     8341 agatagccta gaaggacacc cagttgggac agtcttcata ggcttggcgc aagatcaagg
     8401 aactgaggtt atcaaggtta atattggagg cagaagccga gcagatgtac gtcacattgc
     8461 ggttatgcat gcctttaacc tagttcgcaa ggctttatta agtgactaac ttttgatata
     8521 atagtagata ggtctgagga tcattagaat gtaggagaat agaatggcga aaaaaccaaa
     8581 aaaattagaa gaaatttcaa aaaaatttgg ggcagaacgt gaaaaggcct tgaatgacgc
     8641 tcttaaattg attgagaaag actttggtaa aggatcaatc atgcgtttgg gtgaacgtgc
     8701 ggagcaaaag gtgcaagtga tgagctcagg ttctttagct cttgacattg cccttggctc
     8761 aggtggttat cctaagggac gtatcatcga aatctatggc ccagagtcat ctggtaagac
     8821 aacggttgcc cttcatgcag ttgcacaagc gcaaaaagaa ggtgggattg ctgcctttat
     8881 cgatgcggaa catgcccttg atccagctta tgctgcggcc cttggtgtca atattgacga
     8941 attgctcttg tctcaaccag actcaggaga gcaaggtctt gagattgcgg gaaaattgat
     9001 tgactcaggt gcagttgatc ttgtcgtagt cgactcagtt gctgcccttg ttcctcgtgc
     9061 ggaaattgat ggagatatcg gagatagcca tgttggtttg caggctcgta tgatgagcca
     9121 ggccatgcgt aaacttggcg cctctatcaa taaaaccaaa acaattgcca tttttatcaa
     9181 ccaattgcgt gaaaaagttg gagtgatgtt tggaaatcca gaaacaacac cgggcggacg
     9241 tgctttgaaa ttctatgctt cagtccgctt ggatgttcgt ggtaatacac aaattaaggg
     9301 aactggtgac caaaaagaaa ccaatgtcgg taaagaaact aagattaagg ttgtaaaaaa
     9361 taaggtagct ccaccgttta aggaagccgt agttgaaatt atgtacggag aaggaatttc
     9421 taagactggt gagcttttga agattgcaag cgatttggat attatcaaaa aagcaggggc
     9481 ttggtattct tacaaagatg aaaaaattgg gcaaggttct gagaatgcta agaaatactt
     9541 ggcagagcac ccagaaatct ttgatgaaat tgataagcaa gtccgttcta aatttggctt
     9601 gattgatgga gaagaagttt cagaacaaga tactgaaaac aaaaaagatg agccaaagaa
     9661 agaagaagca gtgaatgaag aagttacgct tgacttaggc gatgaacttg aaatcgaaat
     9721 tgaagaataa gctgttaaag cagtggagaa atccgctact ttttcgattt ttgattcaag
     9781 tttttagatt atatatagta gcttgaaata agatatgaac aact
//
LOCUS       DAVMHG010000068         9780 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMHG010000068 DAVMHG010000000
VERSION     DAVMHG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.068"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VYR08_001315"
     CDS             complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VYR08_001315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV3466835.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1921..2619
                     /locus_tag="VYR08_001316"
     CDS             1921..2619
                     /locus_tag="VYR08_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3466836.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2662..3558)
                     /locus_tag="VYR08_001317"
     CDS             complement(2662..3558)
                     /locus_tag="VYR08_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV3466837.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3567..4502)
                     /locus_tag="VYR08_001318"
     CDS             complement(3567..4502)
                     /locus_tag="VYR08_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV3466838.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYSGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4499..5224)
                     /locus_tag="VYR08_001319"
     CDS             complement(4499..5224)
                     /locus_tag="VYR08_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV3466839.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5308..5943)
                     /locus_tag="VYR08_001320"
     CDS             complement(5308..5943)
                     /locus_tag="VYR08_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV3466840.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQDL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5945..6772)
                     /locus_tag="VYR08_001321"
     CDS             complement(5945..6772)
                     /locus_tag="VYR08_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3466841.1"
                     /translation="MMIKIIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6794..7129)
                     /locus_tag="VYR08_001322"
     CDS             complement(6794..7129)
                     /locus_tag="VYR08_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV3466842.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEGGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7130..7846)
                     /locus_tag="VYR08_001323"
     CDS             complement(7130..7846)
                     /locus_tag="VYR08_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV3466843.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8369..8980
                     /locus_tag="VYR08_001324"
     CDS             8369..8980
                     /locus_tag="VYR08_001324"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV3466844.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9025..9753
                     /gene="rlmB"
                     /locus_tag="VYR08_001325"
     CDS             9025..9753
                     /gene="rlmB"
                     /locus_tag="VYR08_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV3466845.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
BASE COUNT         2967 a         2186 c         1765 g         2862 t
ORIGIN      
        1 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
       61 gtttgaaaac tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa
      121 tttattcttt ttagcaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta
      181 accgttgatt ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg
      241 tggtttactt gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg
      301 tactgagatt ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc
      361 gatagaagtg atagcacgga ttgcaatcgt gtagtcatac gtacgaccgt cacccataac
      421 accgactgag cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc
      481 agctttagcg atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc
      541 ttcagtgatt tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca
      601 tacgatgtgg tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag
      661 agtattgagt ggttcaatca gttcaaactg catatcttct ggaagaccac ccacgttgtg
      721 gtgtgacttg atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt
      781 accttgagca aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac
      841 aaactcgtta ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc
      901 aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat
      961 gtccataact tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat
     1021 caattgatcg ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc
     1081 tgatagacca agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat
     1141 gtcaatgaaa ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt
     1201 acgaaggata tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta
     1261 aatgtgttta tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg
     1321 aacaaagtca gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc
     1381 atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc
     1441 acgatttcca gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg
     1501 cataccataa cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc
     1561 aaatgaacca tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt
     1621 gacttcacga acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat
     1681 ctcacggata cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat
     1741 tttttctaca tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa
     1801 atatcttttc ccaatattct atcacaaata agggcgaatt accaactaaa caaggcaaag
     1861 tttgactttt tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat
     1921 atgctacctg cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt
     1981 tgggctattg gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt
     2041 cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt
     2101 gtaggaagcg gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc
     2161 agttttactg aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac
     2221 aaaaaaacca ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat
     2281 attatccgaa tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct
     2341 tctattcctg aaaaattcat taaggacttt aaaaaagaag aaatcaccag tcatttcttc
     2401 caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc
     2461 cttgctaaag aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga
     2521 ttgacccagg tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat
     2581 gtaggcgaac gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct
     2641 gtcacggaca aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt
     2701 tacagcaaac aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat
     2761 tattaagaag gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt
     2821 gaccggtcct atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat
     2881 aaggataatg cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag
     2941 aacccctgta aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgaccaa
     3001 gctgctcccc cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa
     3061 ggcagaaaag agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc
     3121 atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag
     3181 tgtcggtgcc accctatcct tgatacagct ataaattaaa attccgacag gacaaacata
     3241 ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg
     3301 gttgagaaaa agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt
     3361 taaaaagacc agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc
     3421 agcgattaaa agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta
     3481 ttggccactc gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc
     3541 ttttaaacta tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga
     3601 ttgcggtcta atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa
     3661 atagtggctt cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg
     3721 tctagccaat ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa
     3781 aaattttgat ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt
     3841 aaatccttta tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc
     3901 ttgggatcat gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact
     3961 ggcccaaact tagtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca
     4021 tccaaatctt gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc
     4081 gaataaagaa aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca
     4141 ggggctgtta aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag
     4201 agctggcgaa tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta
     4261 tcaatatcta attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc
     4321 ttactgacac tagctaggtc ataaactagt cctgcctcag tctgcaagcc atgctctggg
     4381 tcactctggc ctgaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct
     4441 ccgggataaa tccctgcctc gatttgttct tctatttttt taataatctt ggtccacttc
     4501 atacttcttc aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag
     4561 acttaggaat aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt
     4621 cattgaccaa gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat
     4681 cctgcccctg agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac
     4741 tttcgatatc agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg
     4801 atgccaaatc tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat
     4861 cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat
     4921 tttgaccttt atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta
     4981 agggattttc caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag
     5041 tacgcatact gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg
     5101 acagaaaggc cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt
     5161 catttaattt tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat
     5221 tcataaattc ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta
     5281 tgctcaaatt tcacattatt tagctattta gtaaagatag tctttcaagg cttttgacaa
     5341 agcctgataa ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg
     5401 gcctgcttgg tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga
     5461 tgcaagatct tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac
     5521 tgcctgctga tactcctctc cctcattgac aggtaaaatg gaaagcaatt taatctctgt
     5581 caatggataa tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt
     5641 cacaggaaca tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc
     5701 tccaccatat agatgagcat caaggttctc tagtaacagt cctgtctgat agccacgaat
     5761 tcctcgattg acaatcattt ttgaagtccc aaatagctcc tgtagaggat aatactcgac
     5821 aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg
     5881 atacttggtt tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc
     5941 cacttcattc tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctat
     6001 tggctggtgc tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat
     6061 aggcaattcc agccatttca agcatttcaa catcattttc actatcacca aaagccatga
     6121 tttcttggga tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa
     6181 tcccagcttg gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat
     6241 caaagagagc attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc
     6301 tcatcttgag cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt
     6361 ggtagaattt ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac
     6421 cttccttgac aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac
     6481 ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca
     6541 attcattgtt ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca
     6601 agggactcag tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa
     6661 tgcccctttg atccaactga tctaaaatct tttccaatcg tgggagatca agctgacctc
     6721 tagcatccag caaggtccca tccatatctg tcgcaattat cttaatcata atttactttc
     6781 ctctggattc aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg
     6841 tatcaattcg ttccttagtc gcttcatcta actggtcacg atgactgcca ccctctataa
     6901 aatcttccaa aatataagtt tggtaatgat acaagggata tccttctggt gaaaaggttc
     6961 cctttggtgt tcgattgacc caagtaggat gagctttagc tgttccgata gttgtttttc
     7021 catccttctt cttgatggtg acatccatga gaacaccacg ttcagtccac ttagcattct
     7081 cttcatctcc catagattca atccgttgat tggaaatgaa gttccccatt taatagataa
     7141 tgagtttctt atctccatct ttttcaaccg tttcagatgg ttcaacaacg tgaggatgcc
     7201 ctccaaagat aatatccgct ccccaatcga tcatcttgtg ataaagagct ttttgttctt
     7261 cagttggttc caatcgatac tcaacaccca tctgaagcat gataatggtg atatctgctt
     7321 ccttctctgc ccgttcaact tcagccttta tcttatcttc gtttaaatct gaaagataac
     7381 gattatagtc ttcctgagaa atatactgct caattccatt gaaaccatag gaataggcta
     7441 acaatgcaac cttgatagca ttcacttcct taatgaccag cggagcctga gcacgtggtt
     7501 cgtgcgtata aactccgatt ggagtgattc cagctttctc aataatatcg gccattgaaa
     7561 taactccctc aatttgcgaa tccaaaatat gattatgagc taaatccagc acatgataac
     7621 ctgcctcctt aatagcatcc ataacttcaa caggagcatt aaagagaaga taacccgcta
     7681 aataatgatc cttattaatg gttccttcaa aatcaccaat agctaaatct gcttgcttga
     7741 gccaaggagt cacatactca aaattttcat gaaagtcata ggtaccgtct tcttttttag
     7801 ctgaaaagaa aagtccatcg tggtagagca aatccccgtg ggccataatt ctggcagttt
     7861 tttcctcttc ctactcctga gacttttgct tagtcccctc ttgagaaata gtatctcgtt
     7921 tttgactagt caaaggattc cttggaagct ttcaaacaac aagaccaagc ccattgataa
     7981 accaactgct agcaagagta tcgccacaaa tcccttattg ctccacttgc gataactcct
     8041 aaaaagttta ccaagccctt cataaaacga aaagctaaac caccctgatt tcgattttgt
     8101 cttctttgta tcttcattct ccctactttc ttatgcaagc cttttctttt tattatatca
     8161 cagataagta tttctttcac aattgaattg aacttcctat ctattttcta taaatcctaa
     8221 atgccataat gctttcaatc cttgtcattt tgtgatatca tgtagaggta atgaaaggaa
     8281 atgaactaat ccacagtggc ttattccaag tataccactt gggctttggc agtagctaac
     8341 tgcactaaac ataatataag gagagaaaat gtctgcaata gaacgtatta caaaagctgc
     8401 tcacttaatt gatatgaacg atattatccg tgaagggaat cctactctac gcgcgattgc
     8461 tgaggaagtc actttccccc tatctgacca ggaaatcatc ctaggcgaaa agatgatgca
     8521 attccttaaa cattcccaag atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt
     8581 tggactggct gctccccagt tagatatctc aaaacgcatt atcgctgttt tggtacctaa
     8641 tattgttgaa gaaggcgaaa ctccacagga agcctacgat ttggaagcca ttatgtacaa
     8701 tccaaaaatc gtctctcact ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct
     8761 gtctgttgac cgtaacgtgc ctggctatgt tgttcgccat gcccgcgtta ctgttgacta
     8821 ctttgacaaa gatggagaaa aacaccgtat caaactcaaa ggctacaact ccattgttgt
     8881 tcagcatgaa attgaccaca ttaacggtat catgttttac gatcgcatca atgaaaaaga
     8941 cccatttgca gttaaagatg gtttactgat tcttgaataa agaaaatccc gttgcaagac
     9001 ggggttttgt gttataatag aggcatgaaa acaaatgata ttgtctatgg cgtccacgcc
     9061 gttaccgaag ccctccttgc aaatacagga aacaaactct acctccaaga agatctccga
     9121 ggtaagaatg ttgagaaagt caaggaacta gctacagaaa agaaggtgtc catttcttgg
     9181 acatcaaaaa aatctctctc tgagattact gaaggtgctg ttcatcaagg ttttgttcta
     9241 cgagtgtctg aatttgccta tagcgagcta gattacatcc ttgcaaaaac acgccaagaa
     9301 gaaaatccac ttctattgat tctagatggt ctaaccgatc cccataatct gggttctatc
     9361 ttgcgaacag ccgatgcgac caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc
     9421 ggagtaactc ctgtcgttgc caaaacagcc acaggtgcta ttgaacacgt tccaattgcc
     9481 cgagtgacca acctcagtca aaccttagat aaactcaagg atgaaggttt ctggaccttt
     9541 ggaacggata tgaacggtac tccttgccac aagtggaata caaaagggaa aatcgccctc
     9601 atcattggaa atgaaggaaa aggtatctct agcaacatca aaaaacaagt cgatgagatg
     9661 attaccattc cgatgaatgg acatgttcaa agccttaatg ccagtgttgc tgcggccatt
     9721 ctcatgtacg aagttttccg aaatagacta taaaaaagtt tccagtcatc tgattggaaa
//
LOCUS       DAVMHG010000069         9743 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMHG010000069 DAVMHG010000000
VERSION     DAVMHG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.069"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            266..361
                     /locus_tag="VYR08_001326"
                     /pseudo
     CDS             266..361
                     /locus_tag="VYR08_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(614..1234)
                     /locus_tag="VYR08_001327"
     CDS             complement(614..1234)
                     /locus_tag="VYR08_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3466846.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1234..1716)
                     /locus_tag="VYR08_001328"
     CDS             complement(1234..1716)
                     /locus_tag="VYR08_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV3466847.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1716..2558)
                     /locus_tag="VYR08_001329"
     CDS             complement(1716..2558)
                     /locus_tag="VYR08_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV3466848.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSH
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2653..3441)
                     /locus_tag="VYR08_001330"
     CDS             complement(2653..3441)
                     /locus_tag="VYR08_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV3466849.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3585..4964
                     /gene="glmU"
                     /locus_tag="VYR08_001331"
     CDS             3585..4964
                     /gene="glmU"
                     /locus_tag="VYR08_001331"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV3466850.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4974..5519
                     /locus_tag="VYR08_001332"
     CDS             4974..5519
                     /locus_tag="VYR08_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV3466851.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5531..5845
                     /locus_tag="VYR08_001333"
     CDS             5531..5845
                     /locus_tag="VYR08_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV3466852.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5862..6554
                     /locus_tag="VYR08_001334"
     CDS             5862..6554
                     /locus_tag="VYR08_001334"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV3466853.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            6631..7122
                     /gene="rocS"
                     /locus_tag="VYR08_001335"
     CDS             6631..7122
                     /gene="rocS"
                     /locus_tag="VYR08_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV3466854.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7220..7810
                     /locus_tag="VYR08_001336"
     CDS             7220..7810
                     /locus_tag="VYR08_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV3466855.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVATFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7819..9582
                     /locus_tag="VYR08_001337"
     CDS             7819..9582
                     /locus_tag="VYR08_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV3466856.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2998 a         1696 c         2090 g         2959 t
ORIGIN      
        1 caaaagttag attttttctg tctaactttt ggggtgtagt tcatgtacac ctgatatgat
       61 gcgttttata attttaaaga ctttttgacc agcctcattt ttttaacttg atactcagtg
      121 aaaagcaaag attaaactag gaagctagct gtaggctgct caaagaacag ctttgaggtt
      181 gcagataaaa cttgtgaggt aaccaacata tataatgtga agctgacgtg gtttgaatag
      241 attttagaag ggtatgagtc tggaagtttt aatggataat gcaagattcc atagaatggg
      301 taagctagag ttcttatgtg aagagtttgg gcataaactt ttaccttttc ctccctactc
      361 atcttagtat agaaaagtga atctaaaata gtacataact gcttctaaaa cattcttata
      421 aattgattta aattctcaaa tcatattatt cagttcttat ttcattttgc tctacaatcc
      481 tgttgagaag acacgtgttc atatcaaaaa ggtattggca agttgcaata cctttttaca
      541 aggttctttt gtcttatttt tgtttcaact gactatatct cctatggttc tagttcagaa
      601 ggctaggcta taattatgat tgataagaag tatcattcca agtattgaga gtgaatgttt
      661 caaaatcatg ggtttctata atggtcaggc tggcatttgc tagaccgcca tctttacgaa
      721 gaagtggttc tttatagcct aggagagtac gaagactggc agtaagattg gcgccgtgtc
      781 cgacaattag aatacgctca gctggactat cttttaatga tttgataaat tggatggtcc
      841 gctgagttgt actatagagg gattcggctc cgaacattcg agtgtcaaat tgagcaagat
      901 ttgaacgaaa agcctggatt tgttgcgggt aaatagcttc cagggttgca attttcaaac
      961 cttctaactt cccaagttgc cattcacgga gattaggaac gatttctaaa gaacaggggg
     1021 tatggagttg actttggata atctcagcag atttgaccgc tcgaggtaaa tcacttgaat
     1081 aaatctgatc aaaaggaatt tccttgagat actggccaag tcgttttagg gtttcaatgg
     1141 attcaggaag aagaggagaa tcaccgctag caccttgaaa acgaccttct tggttccaga
     1201 gggtacgacc gtggcggaca aagtagagtt tcattacttg atgtcctcca aaatatctac
     1261 aaagtctgcc tttacaaagc tagccaagtc ttgtggcgcg acgataatgc tgtgtccgac
     1321 ttcgcctgca gagacaatca ttcgatccaa atctagagca attttatcga taaaaatggg
     1381 ataattgtgt ttctgacgaa ttccgacagg attattggct ccatgaatgt aaccagttgt
     1441 tttttctaag tccttttgtg gaatcatgct cactttttta ttgccagaaa ttttagctag
     1501 tttcttttca gacaagtgct gagtgatagg gacaattccg ataatcggtc cggtcttgtc
     1561 tcccaaaagc gccaaggttt tgaaaatctg atctcgttca taaccttgag gaagctctcc
     1621 ttctagggca ttgatttgaa tcccctgatg agggatagct gctttagata ggatttgttc
     1681 caccaatgtt tttttgattt taactttttt tgccattatt tatatttatc ctccaattga
     1741 ctcatccaaa taccaagcca gattcccagc gcaaagaaga aggcgatgat gacataaccg
     1801 acaagtgaaa gtcctgtgta ttggatactt tcagcgtttc ctgcatttgg aattaagatc
     1861 aaaagggtac ttgataggac gataccgatg atgaaatgat agacgtgtga gtggtagttg
     1921 tttaaggcat aatccatcaa ttttgaaaaa acgatgagag ttgcacctgc accaattcca
     1981 atcggaaaga aggttcccaa gagatcaaaa gttttaaaac cagtcaacat aggagcatag
     2041 agtcccaaaa tcaaaagtaa gttcgatggg ctgaggccag gaaccaagac gccaagggcc
     2101 aatagtgcgc ctgctaggac gaagttaaga aagctggcgc ttaaggttcc aacgacaaaa
     2161 tttaaggcat agagtcctaa tccagaaatg ataaaggttg tccataacca agctaaatca
     2221 atcttgtctc ggtcagattc tcgagttgat tctttgagga ggctaggaac tgtaccgata
     2281 atagctcccg caaagctcca taatacaaag acctgataat tttcaagcag gtattcaatc
     2341 gggtaggaaa ataagccgat tcccagaagc ataccgatgg caactggaat aaagtacaaa
     2401 acattttctt taaggtcttt aaagggatgg gccagaaagc caatcattcg ttcatagatt
     2461 cctaagattg ctgctagaac ccctccggaa attcccggta ggataaatcc aagagcaatt
     2521 acaatccctt taataacgcg tgctaaccat gagagcatat atttcctcca actatttcta
     2581 tttcaaaaaa tccttactat attgtatcac aatcagacac aaaaagaaaa agcaaatgat
     2641 aaacaaatgc ttttaaggtt ttaaaaaaag ttttcgaaag gtttcttctt tattttttaa
     2701 gggagagata acgttgatat ctaaatcgtg gtcaaagccg gcaatttttc ctttagatgt
     2761 gtattggtga atatcataat ctaaatcagt tttaggactg ctctccaaaa atcctgagtc
     2821 tgagccgtag gacggaatcc aaacagaggt aaacttgcct gtatcaatac tgtgttcttc
     2881 catgaagtag acaccaacgt agatgccgat gtttttagca cctagtgatg ctagttttgc
     2941 tcgaaagttt tcgacacctt cgttcatatt agacatggtt ttgtcttcca cgtcaagcca
     3001 atagtaacta gggctgtaag gagaggcagc attgtagaaa acttcggcag ccttttccat
     3061 ttcttggaca ctttttccag ctacataagc gtagacagca actgggacat tccgcttttg
     3121 aagttcagtg atatgactct tataggcctt gtctattcca ttgataaatg aagcatcatt
     3181 ttcttttgtt gtttgagcac cactgtgaac acgaacaata gcacctgaaa tattttgtga
     3241 gagggcatcg tagttgattt cctcaggacg ctgccagcca gagaggtcaa taatcggttt
     3301 gtctaagtgt ttcaaagcct gtgctatatt ggattttgtt ttaaacgatt ggctgtcatt
     3361 aagtgggcga ttgatgatta aaatgaacat cataatccca aaaaaactaa ataaaataag
     3421 tggatgaatt tgttttctca tatcttataa gtctacccta aaaatcaaaa aaaatcaaaa
     3481 aaatgggtta aggaagagac tttagagcat tttttcattc aagagtgcgg aatgatttga
     3541 aatatggtat aataaaaggg aatttctaca gaaaagagaa gattatgtca aattttgcca
     3601 ttattttagc agcgggtaaa gggactcgca tgaaatctga tttgccaaaa gttttgcaca
     3661 aggttgcggg tatttctatg ttggaacatg ttttccgtag tgtgggagct atccaacctg
     3721 aaaagacagt aacagttgta ggacacaagg cagaattggt tgaggaggtc ttggctggac
     3781 agacagaatt tgtgactcaa tctgaacagt tgggaaccgg tcatgcagtt atgatgacag
     3841 agcctatctt agaaggtttg tcaggacaca ccttggtcat tgcaggagat actcctttaa
     3901 tcactggtga aagcttgaaa aacttgattg atttccatat caatcataaa aatgtggcca
     3961 ctatcttgac tgctgaaacg gacaatcctt ttggctatgg acgaattgtt cgtaatgaca
     4021 atgctgaggt tcttcgtatt gttgagcaga aggatgctac agattttgaa aagcaaatca
     4081 aggaaatcaa cactggaaca tacgtctttg acaacgagcg tttgtttgag gctttgaaaa
     4141 atatcaatac caataacgct caaggcgaat actatattac agacgtcatt ggtattttcc
     4201 gtgaaactgg tgaaaaagtt ggcgcttata ctttgaaaga ttttgatgaa agtcttgggg
     4261 taaatgaccg tgtggcgctt gcgacagctg agtcagttat gcgtcgtcgc atcaatcata
     4321 aacacatggt caacggtgtt agctttgtca atccagaagc aacttatatc gatattgatg
     4381 ttgagattgc ttcggaagtt caaatcgaag ccaatgttac cttgaaaggg caaacgaaaa
     4441 ttggtgctga gactgttttg acaaacggta cttatgtagt ggacagcact atcggagcag
     4501 gagcggtcat taccaattct atgattgagg aaagtagtgt tgcagacggt gtgacagtcg
     4561 gtccttatgc tcacattcgt ccaaattcaa gtctgggtgc ccaagttcat attggtaact
     4621 ttgttgaggt gaaaggatct tcaatcggtg agaataccaa ggctggtcat ttgacttata
     4681 tcggaaactg tgaagtggga agcaacgtta atttcggtgc tggaactatt acagtcaact
     4741 atgacggcaa aaacaaatac aagacagtca ttggagacaa tgtctttgtt ggttcaaatt
     4801 caaccattat tgcaccagta gaacttggtg acaattccct cgttggtgct ggttcaacta
     4861 ttactaaaga cgtgccagca gatgctattg ctattggtcg cggtcgtcag atcaataaag
     4921 acgaatatgc aacacgtctt cctcatcatc ctaagaacca gtaggagcct atcatggagt
     4981 ttgaagaaaa aacgcttagc cgaaaagaaa tctatcaagg accaatattt aaactggtcc
     5041 aagatcaggt tgaattacca gaaggcaagg gaactgcccg acgggatttg attttccaca
     5101 atggggctgt ctgtgtttta gcagtaacgg atgaacaaaa acttatcttg gtcaagcagt
     5161 accgcaaagc tatcgaggct gtctcttacg aaattccagc cggaaaattg gaagtaggag
     5221 aaaacacagc ccctgtggca gctgcccttc gtgaattaga ggaagaaaca gcctatacag
     5281 ggaaattaga actcttgtac gatttttatt cagctattgg cttttgtaat gagaagttaa
     5341 aactatattt agcaagcgat ttgacaaaag tggaaaatcc gcgtccgcag gatgaggatg
     5401 aaaccttgga agtccttgaa gtgagcttag aagaagcgaa agaattaatc caatcaggtc
     5461 atatctgtga tgccaagaca attatggctg ttcagtattg ggagttgcag aaaaaataga
     5521 ggaggtcagt atgggtaaat ctttattaac ggatgaaatg attgaaagag ctaatagagg
     5581 cgaaaaaatt tcaggtcctc ctttgctaga tgataatgag gaaactaaga ttttaccaac
     5641 ctcttcttcc cgttttggtt atgccaatcc taaggatcat ggttttagcc aggaaacctt
     5701 gaagattcag gtcgaaccat ctattcataa aagccgtcgc attgaaaata ccaagagaaa
     5761 tgtcttcaat tctaagttga ataaaatctt attcgcggtc atctttctct tgattttgct
     5821 tgttttagca atgaaacttt tgtaatagaa aaggaattga aatgaaaata ggaattattg
     5881 ctgctatgcc agaagaactg gcttatctgg tccagcattt agacaatgcc caggagcaag
     5941 ttgttttggg gaatacctat catacaggaa ccattgcttc tcatgaagtc gttcttgtag
     6001 aaagtggaat tggtaaggtc atgtctgcta tgagtgtggc gattttggct gatcatttcc
     6061 aggtggatgc ccttattaat acgggttcag ctggggcagt agcagaaggt atcgctgttg
     6121 gggatgtcgt gattgctgac aaattagcct atcatgacgt ggatgtcaca gcttttggct
     6181 atgcttatgg acaaatggcg caacaaccgc tttatttcga atcagacaaa acctttgttg
     6241 ctcaaatcca agagagttta tctcaattgg accaaaactg gcatcttggt ttgattgcta
     6301 caggagatag ttttgttgca ggaaatgaca agatagaagc gattaagtcc catttcccag
     6361 aagttttagc cgtggagatg gagggggcag ctattgctca agcagcgcat gccctcaatc
     6421 tcccagtctt agtcatcaga gctatgagtg acaatgccaa ccatgaagca aacatctttt
     6481 ttgatgagtt tattatcgaa gctggacgtc gctctgccca agtcttgttg gcctttttga
     6541 aggctttaga ttaagcggaa atttgacagt ttttctagct tatgataaga tttaagtaaa
     6601 gaaaagctag aaaacgtttc agaggatatt atgagtattg aaatgaccgt cagtgagatt
     6661 gcagaggtct taggattatc tcgccaagca atcaataacc gtgtcaaaga attaccagaa
     6721 gaagacacag ataaaaatga caaaggggta acagttgtta ccagaagtgg cttgattaag
     6781 ctagaagaaa tctataaaaa aacgattttt gaagatgagc ctgtcagtga agatgtcaaa
     6841 caacgtgaac tgatggagat tctagtggat gagaagaatg cagaaattct tcgtctttat
     6901 gaacaattaa aagccaagga tcgtcagtta tcagaaaaag acgagcagat gcgtatcaaa
     6961 gaccgtcaga ttgctgagaa agacaaacaa ttagatcagc aacaacaatt gacccttcaa
     7021 gccatgaagg atcaagaaaa tcttaagcta gagctggacc aagcaaaaga agaagtccaa
     7081 tccactaaga aaggcttttt tgctcgttta tttggaggat aatcaaggag ctgtttaggt
     7141 taaatgctaa cctgatggct tcttttgttt gtgaatagta tagttgatta gaacagagac
     7201 agtagaggag aggtacagaa tggaacgatt agaagattac attgcttttg atttagaatt
     7261 taatcagcac gaaggacaaa cacacttgat tcaagtatcg gcagtgcgtt ttagagatgg
     7321 tcgggaaatc gatgcctatg attcctatgt tcataccagc gtccctctaa aaagctttat
     7381 caatggtttg accgggatta cagctgagac cttaaaagat gctcctccag ttgagaaagt
     7441 cttagaagaa ttccaagagt ttgtgggttc tttgctgatg ataggctaca atgctgctaa
     7501 aagtgattta cctattttag cagagcatgg tttagactat agccaacagt acaaggtgga
     7561 tttgtatgat gaagcttttg aacgtcggag ttcagattta catggtattg ccaatctcaa
     7621 attgcaaact gttgccacat ttcttggatt tcaaggtcag tctcataata gtttagagga
     7681 tgctcggatg acggcgcgtg tttacgaatc cttcttagag tcggatcaag ctagggaatt
     7741 gttagagaca gaaagtagct tatcaaacaa tccttttggt ggcttggact tgtctcaatt
     7801 atttgactag gagtgcctat gaaacctgaa aaacctaaaa aattgggtct taaacagatt
     7861 tacctcaatc ttgataaaat cctctttcta tttttcttga tttttatgat agtggagtat
     7921 ttgtgggtac cattcaattc atggttggct agcttcttat tagatcagac aggttatctt
     7981 tttatttcct ataacaatgt cttagccatc cttactcatt ctccattgat tagtctagct
     8041 tttttagcct tgattgtaat taaccttctg gttgcttatt ttcaaatagg actcctcttt
     8101 attggtgctc gtcatctcct ctataatgaa aagcgaaggc taattgagta tatccgcaag
     8161 gttttccatg aaagttttgg atttatgaaa aagctaactt gcgctaaagc cttgtttatc
     8221 tttttctata tagctatgct gtttcctttt atacggaaaa tgctgaagat ttattatctt
     8281 aataaaatcg tcattccaga gtttatccaa gcttatttag aagatagata ttggatgtgg
     8341 tggctgagca tcctacttct atctcttatc tttctctatg tctctgtcag attgatgttt
     8401 gcccttccga agattgtata tgatcagctg actgttcgag aagcagtgat gtttagcttg
     8461 gagaaaacca agaagagagt tactttttat gcttggaatt tattttacat cttgctcaaa
     8521 gcacatttat tattttatct tcctttgatc cctttattat tggctcaaac tttggtagat
     8581 gatatcactc agagagagtc tctgattctt ggtattttga attttgtcgt gattaaaaat
     8641 ctctattata tggctctgac ttattttttg gttaaatttg tttcattttt gacaggaaag
     8701 gagctggata tgcttcctag gagagaaaaa gatcacatcg tgcgatgggg tgtcatgact
     8761 tgtgccagcc ttttctttgc cttagaaggt tatatttatc tggaggctcc catggttcat
     8821 ctacctcaac ttatttctca ccgaggggtt tccaatgcaa atgggattca aaatacagta
     8881 gagtccttgg aaactacagc acaactcaaa ccagacttgg tggagacgga cgtgcaggaa
     8941 acaaaagatg ggcagtttgt catgatgcat gatgctaact tgaaaaatct agcaggtatc
     9001 aataaaagtc ctcaagactt aaacttggag gagcttaaag ggattgatat ttttgaaaat
     9061 ggctaccaga ctaaaatttc aagctttgaa gattatctca gtcgagccaa cgaacttggt
     9121 caaaaattac taattgaaat taaaaccagt aaaaaagata gtccagacat gatgaaccgc
     9181 tttttagccc gttatgctgc aaagctcaag atttatggac atcaaatcca gtctttagac
     9241 taccatgttg tcgaaaaagt aagacagtat gatgccgaac tgccagttta tttcatcatg
     9301 ccctacaatt ctgtctttcc taaaacaaga gcgacaggat atactatgga gtactcaacc
     9361 ttggatgaat attttgtaag caaactatgg acaacggatc agaaacttta tgtctggact
     9421 gtcaatgatt cagaagctat cagcaaatct cttcacttag gagtagatgg agtgattaca
     9481 gatgatttgg aaaaagttca gcgggaaata gaagtagccc aagaagatcc agaatatacg
     9541 gatttgctct tgaaaaaagc attggaattc tttgaatttt agtaataaaa aagaggaacg
     9601 gttgttcctc ccatgatagg taagtattct tgtgaatgct tgcttttttg tggttgcaat
     9661 ccgtttacga gaattgtttg tactgttctt gtttcaattt actttatagt gctttgaagt
     9721 tggaatgcta cactataact tct
//
LOCUS       DAVMHG010000070         9665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMHG010000070 DAVMHG010000000
VERSION     DAVMHG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.070"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(32..985)
                     /locus_tag="VYR08_001338"
     CDS             complement(32..985)
                     /locus_tag="VYR08_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV3466857.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(1053..2262)
                     /gene="sstT"
                     /locus_tag="VYR08_001339"
                     /pseudo
     CDS             complement(1053..2262)
                     /gene="sstT"
                     /locus_tag="VYR08_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            2401..2961
                     /locus_tag="VYR08_001340"
     CDS             2401..2961
                     /locus_tag="VYR08_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV3466858.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            3018..3926
                     /locus_tag="VYR08_001341"
     CDS             3018..3926
                     /locus_tag="VYR08_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV3466859.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            3878..4468
                     /locus_tag="VYR08_001342"
     CDS             3878..4468
                     /locus_tag="VYR08_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV3466860.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            4471..5577
                     /gene="yqeH"
                     /locus_tag="VYR08_001343"
     CDS             4471..5577
                     /gene="yqeH"
                     /locus_tag="VYR08_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV3466861.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTDDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAREEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            5792..6103
                     /gene="yhbY"
                     /locus_tag="VYR08_001344"
     CDS             5792..6103
                     /gene="yhbY"
                     /locus_tag="VYR08_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV3466862.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            6155..6784
                     /locus_tag="VYR08_001345"
     CDS             6155..6784
                     /locus_tag="VYR08_001345"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV3466863.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            6784..7377
                     /gene="yqeK"
                     /locus_tag="VYR08_001346"
     CDS             6784..7377
                     /gene="yqeK"
                     /locus_tag="VYR08_001346"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV3466864.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            7378..7878
                     /locus_tag="VYR08_001347"
     CDS             7378..7878
                     /locus_tag="VYR08_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3466865.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            7891..8244
                     /gene="rsfS"
                     /locus_tag="VYR08_001348"
     CDS             7891..8244
                     /gene="rsfS"
                     /locus_tag="VYR08_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV3466866.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            8564..9304
                     /locus_tag="VYR08_001349"
     CDS             8564..9304
                     /locus_tag="VYR08_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV3466867.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFSMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            9345..>9665
                     /locus_tag="VYR08_001350"
     CDS             9345..>9665
                     /locus_tag="VYR08_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEV3466868.1"
                     /translation="MNIPIPFGDILEKEVITNIPKIYQKLKQIYNDLQFKTEEELGVV
                     YQEYLQFTYKKYSTVKTLLYKNEGKFLYDFYEHVYLDDNNSDLIETSNTELIFEKTSN
                     VILTG"
BASE COUNT         2950 a         1856 c         2212 g         2647 t
ORIGIN      
        1 gcaaggcgac gctgacgtgg tttgaagagt attactgtct atatttttgg taaaaatcaa
       61 cttttacttg gatgaaggtt ttggcttcac gtaggagttg aagaagggtg gcgcgggttt
      121 caaattcttc tcttgtcttg ggcagactgc ggttccggaa gacttccaga taacgttcaa
      181 tttcatctag caaatcagaa gcaggattgg tctggctcag ttgacctgca atttttgaaa
      241 agagttgcgc taagatcagg ctttcactgg cggcaaggtg acaagtgtta atctgttggg
      301 ccatgtttct caggatacga ctttgtcgct gtctcatctc aaagtagtgg atatggtagt
      361 ctgtctggtg aaagaggtgg tcagagtgat ccaaatagac cagtctgagg gcttctttca
      421 aaagcgtgtc taattctgct accagctgtg ctcggttgcg tccgtctcct ctggataaat
      481 agtatttgaa gcgctggagg atatctttta acttttcttc caccagcgtg tggtagtgct
      541 ggatttcctc ttctcgtgaa ggcatataga gattaacaag caaggcaaat cctgtaccaa
      601 tagcaaagag aaggaattca ttgactagaa ggtctggaga ggttgactct tgaaccaaga
      661 gatggctaac caaaacagtg cttggtgtga tgccaatttc ccagcccatc ttgtaggcta
      721 aaggaacgta gaaggccaga tagaggccga gactccagat atgaaatccg ctcaagtgaa
      781 aagctagaac accgatagcc agagctagaa gcatagaaaa aagacgattg cgagccagtt
      841 ttaaagtact tctacgcgta tcagataggc tcaagagagc gataattcca gccgaaactg
      901 ctgacgaaag attgagaaaa taagcaagca ggcaggcaag acaggtagct aagatgagct
      961 tggtcgtacg ttggctaata gacataagaa tttcctaata agttagaata aaagcgtaaa
     1021 agacaagaca tgagcaggct tgccttgatg agttattttt tacgggttgc tgcgtattcg
     1081 gcaacggcgg taaagaggac atctgtagaa gagttaaggg ctgtttcaca tgagtcttgg
     1141 atgacaccaa tcacaaaacc aaccccaaca acttgtatgg caatatcgtt agaaataccg
     1201 aaaaggctac aagcaactgg gataagaagg agggaacctc cggcaatacc tgaagcacca
     1261 caggctgaga tagctgctac cacactgagg acaaaggctg tggcaaagtc aacaggaatt
     1321 ccaagagtgt taactgtaac aagggtcaaa aggttaatgg taatcgctac tccagccata
     1381 ttgatagtag aaccgagtgg gatagaaaca gaataggtat ctgggttgag tccaaggtca
     1441 tggcagagtt tcatgttgac aggaatgtta gtcgcagaac tacgagtgaa aaaggctgtc
     1501 acaccgctga cacggaggca gttccaaact agagggtaag gattgcgtct cataaagaag
     1561 aaggcaatca aagggttgac cacaggggca acaaaaagca tagtcgttac taatggaacc
     1621 aataaaatac catagttggc aaggcttccg actcccttgt cagaaatggt tttaaaaaca
     1681 agaccaagga ttccaaatgg agccagattg atgatccatt cgacaatttt agaagtcacg
     1741 tcagcgatag tttttagcaa ttctttacta tttttactgg cttctctcat agcgattcca
     1801 aaaatgactg cccaagataa gattccaata tagttagcag taaacagggc gttgactggg
     1861 ttgtcaacca gtttgagcaa gaggttgctg aggacctgcc caatcccatc tggtggtgca
     1921 atttcagtat tggcactatt tagggtaatt tcaataggga cgatgaaact tgctagtaca
     1981 gctacaagag cagcggcgaa agtccctatc ataggatata caagaaaaca acagttttca
     2041 tattgctatc ttgtcccttt tgatgttggg aaagggcatt ggcaacgagg gcaaagacta
     2101 ggataggagc aacagctttt agacctccaa cgaataaatc cccgagtagc ccaatccctg
     2161 agagattagg aagggtcagt cctaggattc cccataaagc ataccaatca agatacgctt
     2221 gacaaggctt gccttattcc aagcatgaat gattcttttc ataataatct cctttttgtg
     2281 tagtgattat gattatagta taaatgatag acaaaatcaa gaattttctg tctatttttt
     2341 gaatatttat ggagaatgag actgatgaaa atatggtata atgaaataaa ggagttttat
     2401 atgcaaaaat ttattcaggc ttatattgaa aagctagatg tgacaaccat tatcgagaat
     2461 attctaacca aggtcatttc tcttttactg cttttgattg tattttatat tgctaaaaaa
     2521 atgcttcata ccatggtgca gagaattgtc aaaccttctc taaaaatgtc tcgtcatgat
     2581 gtcggacgcc aaaaaaccat ctcacgttta ctagaaaatg tgtttaatta tacgctatat
     2641 ttctttttac tctactgcat tttgtcgatt ttaggtttgc cagtttctag tttgctggct
     2701 ggagctggta ttgctggggt agcgattggt atgggagccc aaggctttct atctgatgtc
     2761 atcaatggct ttttcatcct ctttgaacgt caactggatg tgggagatga ggtcgttctg
     2821 acaaatggac cgattactgt atcgggtaag gttgtcagtg tgggaattcg tacgacacag
     2881 cttcgtagcg aggagcaagc ccttcacttt gtccctaacc gaaatatcac agttgttagc
     2941 aatttctcac gcacagacta gacctgttat tttaagtaat ttgtggtaca atagagggag
     3001 tttaataagg agaaaagatg gttttagaaa agcagttggg caatggttgt acctggatag
     3061 acctagacct aggaaagttg aataaactag aagacctttc tgaaatttac ggtttggaca
     3121 aggaaaccat tgaatacgca ctggatagaa acgagcgcgc ccacatggac taccaccgtg
     3181 aaagtgggac ggttaccttt atctataatg tcctagacgt aaaaaaggac aaggcctact
     3241 atgagacttt tcccatgacc tttatcgtcg agcatcgtcg cctgattacc attagtaata
     3301 ccaagaacgc ctatgtcatt gaacagatga ctcgttatct ggagaaccat gacacgcttt
     3361 cgatttataa gtttctcttt gccagtctgg aaatcatcag caatgcctac tatcctgtca
     3421 ttgagcagat ggacaagagt agggatgagg tcaatgacct cttgcgccag cgaactacca
     3481 agaaaaacct ctttgccctg tctgatttgg agactggtat ggtttatctg acggcagctg
     3541 ccaaacaaaa tcggattttg ttagagcata ttcaaggtca tgccttgtat cgtagttttg
     3601 atgagattga gagagaacag tttgatgatg ccatgattga ggctcatcag ctggtatcca
     3661 tgacagacct aatctctcag attttacagc agctttcagc ctcttacaac aatattctaa
     3721 acaataatct gaatgacaat ttgacaacct tgactatcat ttcagtcttg ctagctgttt
     3781 tggcagtcgt gacaggcttt ttcggaatga atgttccctt acctttaaca gatgagcccc
     3841 atgcttggct ctatatcagt ttggctagtg caggtttgtg gattgtttta tccttgttac
     3901 taaggaaaat tgcgaaaaaa agttaagaaa aggagccaga atggcgattg aaaattatat
     3961 accagatttt gccgtggaag cagtctatga tctgacagtc ccaagcctgc aggcgcaggg
     4021 aatcaaggct gttttggtcg atttggataa taccctcatt gcttggaaca accctgatgg
     4081 aacgccagag atgaagcaat ggctacatga ccttcgggac gcgggtattg gcattatcgt
     4141 agtgtcaaat aacaccaaaa aacgcgttca acgagcagtt gagaaatttg ggattgatta
     4201 cgtttactgg gccttgaagc ccttcacatt tggtattgac cgtgctatga aggaattcca
     4261 ctatgacaaa aaggaagtgg tcatggttgg cgaccagctc atgacagata tacgagcagc
     4321 ccaccgtgca gggattcggt caattttagt caaacccttg gtccaacatg actcaatcaa
     4381 aacgcagatt aaccgaactc gtgagcgtcg tgttatgaga aaaatcactg aaaagtacgg
     4441 accgattaca tataaaaaag gaatttaact atggaagaaa ttctctgtat tggttgtgga
     4501 gcaaccattc agacgacaga taaggctggt cttggtttta ccccccagtc ggcacttgaa
     4561 aaaggtttgg agactggcga agtctattgc caacgctgtt tccgtctccg ccactacaat
     4621 gaaatcacag atgtccagtt gacggacgat gatttcctca agctcttgca cgaggtggga
     4681 gacagtgatg ccttagtggt caatgtcatt gatatctttg attttaatgg atctgtcatc
     4741 ccaggtttac cacgtttcgt ctcgggcaat gatgtcctct tggtaggaaa taaaaaagat
     4801 atccttccta agtcagttaa gtctggtaag attagccagt ggctcatgaa acgtgcccgt
     4861 gaagaaggtc ttcgtccagt cgatgtggtc ctaacttcag cacaaaataa acatgccatt
     4921 aaggaagtca ttgacaagat tgaacactac cgtaagggcc gcgatgtcta tgtggtcggt
     4981 gtgaccaacg ttggaaaatc aactctaatc aatgctatta tccaagaaat cacgggtgat
     5041 cagaatgtca tcactacttc acgcttccca gggacaacct tggacaaaat agagattccg
     5101 cttgacgacg gatcttatat ttacgatacg ccgggaatta tccaccgcca ccagatggct
     5161 cactacttga cggccaaaaa cctcaagtat gtcagtccta aaaaggaaat caagcctaag
     5221 acctatcagc ttaatcctga gcaaacccta tttttaggtg gtttgggacg ctttgacttt
     5281 atagcaggag aaaagcaagg atttactgct ttctttgata atgaactcaa actccatcgt
     5341 agcaagcttg aaggagctag tgctttctac gataagcacc tgggaactct tctgacacca
     5401 ccaaatagca aggaaaaaga agatttccca aggctagtcc agcatgtctt taccattaaa
     5461 gataagacag acctagtcat ttcaggccta ggctggattc gtgtaacagg cacagcaaaa
     5521 gtcgccgtct gggcaccaga aggcgtcgcc gtcgtcacac gaaaagcaat tatttaagca
     5581 cagaaaggaa agggttgtct gaatttgggc gagcaaggcg agccccatag agaatacttt
     5641 tcgctgtggt gtaagttggt acaagtgatt gtaccaactg cggaaaattt gagaccttag
     5701 gctcaaattt tagtcatgaa agtccgaagg actttgctga cgtccgtcac cacttcagaa
     5761 aagtataaaa agaaactctt ttaaagaaat tatgtcatta acatcaaaac aacgtgcctt
     5821 cctcaacagc caggcacaca ccctcaaacc tatcatccaa atcgggaaaa atggactcaa
     5881 cgaccaaatc aaaaccagcg tccgtcaagc tcttgatgcg cgtgaattaa tcaaggttac
     5941 tctcttacaa aacacagatg aaaacatcca cgaagtagct gaaattttgg aagaagaaat
     6001 cggtgtggat acagtccaaa aaataggacg catcttgatt ttgtttaaac aatctagcaa
     6061 gaaagaaaat cgcaagattt ctaagaaagt caaagaaatc taagaccgaa actccaaata
     6121 actgttttta tagagaaata aaggggacta gcctatgaca atcgaactat tgactccctt
     6181 taccaaggta gagttggagc cagaaatcaa ggagaaaaaa cgcaaacaag ttgggatttt
     6241 aggggggaat tttaaccctg ttcacaatgc ccatctcatt gttgcggatc aagtacggca
     6301 acagttggga ctggatcaag ttcttctcat gcctgaatac caacctcctc acgttgataa
     6361 aaaggaaacc atccctgaac accatcgtct caagatgctt gagttggcaa ttgagggaat
     6421 tgacggccta gtcattgaaa ccattgagtt ggagcgcaag ggtatttcct acacctacga
     6481 caccatgaag attttgacag agaagaatcc agatacggat tattacttta tcatcggtgc
     6541 cgacatggtt gactatctgc ctaagtggta ccgaattgat gaactggttg acatggttca
     6601 gtttgtgggg gttcagcgtc cacgctacaa ggtagggact tcctatccag ttatctgggt
     6661 ggacgtaccg ctcatggata tctcgtccag catggtgcgg gacttccttg cccaaggtcg
     6721 gaaacccaac tttctcctac ctcagcctgt gctagactat atcgagaagg aggggctcta
     6781 ctgatgtcct atcaagacta tatcaactgc tcccgtgagg ccttgttgga aaaaatggca
     6841 gagcttctac ctgagaaacg tctaacccat tgtctaggtg tggagcgtgc cgctatggaa
     6901 ttagctcagc gctttggtgt agatgttgag aaagcaagtc tagcaggtct tcttcatgac
     6961 tatgctaaaa agttgtcaga tcaggaattt ctagccttga ttgaccgtta ccagctagac
     7021 cctgacctca aaaactgggg caataatgtc tggcatggta tggtcggaat ttacaaaatt
     7081 caggaagatt tggatttgca tgattctgaa attctgcgag ccattgaaat tcatacagtc
     7141 ggagctggtc aaatgacaga cctagataag gtcatctacg tcgcagacta tatcgagcac
     7201 aatcgggcat ttcctggagt ggatgtggcg cgtgagattg caagtctatc gctcaataag
     7261 gcggtggcct acgaaacagc tcgtaccgtg gagtatctag ctcatcaggg attccccatc
     7321 tatccccaaa cccttgaaac ctataacgcc tttgtgcact atttgaaaga ggactaaatg
     7381 aagacggctt ttataatcat tgatgttcag aatgttttag tagaaactgg gttccagaca
     7441 aaaagtctat tggaaaaaat ttcttattta caaaaccagg ctagaagcaa gaatattgaa
     7501 attatctatg ttcaacatat tgagaactct gaagctcaaa catcagaaga ttggcagtta
     7561 tctgcgcttt taaatcgaaa acctgctgaa aaggtctttc agaagaagta taacagtatt
     7621 ttcaaagaaa ctggcttaaa agaatacttg gataaacagg ggattgaaaa attagtttta
     7681 tgtggtatgc agacagaata ttgtgtggat acctctgtca aggttgcctt tgaatatggc
     7741 tatcagctta ttgttccaga aggtgctgtc acaacctttg atggggatga cattccagca
     7801 gaaacgatta atgaatttta tgaggacatt tgggaggagc gctttgcaga tgtcctagat
     7861 tacaaacata ttttctaaaa agaggattaa atgaacgaaa aagaattact agaactagtc
     7921 gtgaaagcgg ctgatgagaa acgtgcggag gatatcctcg cacttgatgt acaagatttg
     7981 actagtgtga cggactactt tgtcatcact agctcaatga atagccgtca gttggacgct
     8041 atcgcagcta atatccgtga aaaagtagct caagcaggct ttaaaggtag ccatgtcgaa
     8101 ggcgatgcag ctggaggctg ggtcttactg gacctcggtg ctgttgtcgt gcatatcttt
     8161 tcagaagaaa tgcgtgccca ttataatcta gagaagctat ggcatgaggc gaattcagta
     8221 gatatttcag aagctcttgc ttagaagatg aactcagttg tagtcaactg ggtttctttg
     8281 cttattttag aaaaattgat agaaaggtaa agggcctcgt gtttgtttca tttcgaaact
     8341 gaacacgagc taaaagctga gataaaaagt aactttcttt gtatctgacg atagggaaga
     8401 aagtttccta tgtactcttt gcttttaagg ctctttgtat cttagaaagg atttgaaccc
     8461 gtcctgtaat tctcggaaaa tagatagcct tccttggagc tcggctccat cgtaaggctc
     8521 ctaattttct atcgaattac gggcggactt ggtatcatgt tttatggcga cttatgaaac
     8581 ctttgcggcg gtctatgatg cggtcatgga cgatagttta tacgacaaat ggacgaattt
     8641 ttctctgcgt catttgccta agaccaagga gagaaagaaa ctcttggaat tggcttgtgg
     8701 gacaggaatt caatcagtgc gcttctctca ggctggtttt gatgtgactg gacttgactt
     8761 gagtgcggat atgttgaaga ttgcggagaa gagagcaact tcagccaagc aaaagattgc
     8821 ttttatagaa ggtaatatgc tgaatttgtc caaggcaggg aaatacgatt ttgtcacgtg
     8881 ttattcggac tctatctgct atatgcagga tgaggtggaa gtaggggacg tctttaagga
     8941 cgtgtacaat gtgctgaatg aagagggagt tttcatcttt gacgtgcact cgacctacca
     9001 gacagatgaa gtattccctg gctattccta ccatgaaaat gcggaggatt tttccatgct
     9061 ttgggataca tatgaggacg aagctcctca ctccatcgtg catgagctga ctttctttat
     9121 caaggaggct gacggttcct ttagtcgcca cgatgaagta catgaggaga ggacctatga
     9181 gatcttgacc tatgatatct tgctggaaca ggctggattc aagtccttca aactctatgc
     9241 agactttgag gataaggagc caacagaaac tagcacccgt tggttttttg tggcgcagaa
     9301 gtaaatcaaa tattgttaat cttatgctta ggaggtaagg gaaaatgaat ataccaatac
     9361 catttggtga tattcttgaa aaagaagtaa ttaccaatat acctaaaatt tatcaaaagt
     9421 taaagcaaat ttataacgac ttacagttta aaacagaaga agagttaggt gttgtttatc
     9481 aagaatattt acaatttact tataaaaaat attccactgt aaaaacattg ttgtataaaa
     9541 atgaagggaa atttttatat gatttctatg agcatgttta tttagatgat aataattcag
     9601 atttaataga aacctctaac acagaattga tttttgaaaa aacatctaac gttattttga
     9661 cagga
//
LOCUS       DAVMHG010000071         9638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMHG010000071 DAVMHG010000000
VERSION     DAVMHG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.071"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..2227
                     /gene="blpA"
                     /locus_tag="VYR08_001351"
     CDS             74..2227
                     /gene="blpA"
                     /locus_tag="VYR08_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEV3466869.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2238..3599
                     /locus_tag="VYR08_001352"
     CDS             2238..3599
                     /locus_tag="VYR08_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV3466870.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            3656..3811
                     /gene="blpC"
                     /locus_tag="VYR08_001353"
     CDS             3656..3811
                     /gene="blpC"
                     /locus_tag="VYR08_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV3466871.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(3855..5195)
                     /locus_tag="VYR08_001354"
     CDS             complement(3855..5195)
                     /locus_tag="VYR08_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3466872.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(5209..5946)
                     /locus_tag="VYR08_001355"
     CDS             complement(5209..5946)
                     /locus_tag="VYR08_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466873.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRFLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(5951..6289)
                     /locus_tag="VYR08_001356"
     CDS             complement(5951..6289)
                     /locus_tag="VYR08_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466874.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6566..6895
                     /locus_tag="VYR08_001357"
     CDS             6566..6895
                     /locus_tag="VYR08_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV3466875.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7063..8112)
                     /locus_tag="VYR08_001358"
     CDS             complement(7063..8112)
                     /locus_tag="VYR08_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3466876.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LGWDYLISQESKRKQVLLRFFALFTQVKGISNSVNRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8109..8840)
                     /locus_tag="VYR08_001359"
     CDS             complement(8109..8840)
                     /locus_tag="VYR08_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3466877.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            8908..9318
                     /locus_tag="VYR08_001360"
     CDS             8908..9318
                     /locus_tag="VYR08_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV3466878.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9328..9615
                     /locus_tag="VYR08_001361"
     CDS             9328..9615
                     /locus_tag="VYR08_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466879.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         3179 a         2060 c         1702 g         2697 t
ORIGIN      
        1 ttcaagattt cataaaatat gaacttagtt ttatggcata atatacctat ccactatatg
       61 aaaggaattg ccaatgactt cttataaacg tacatttgtt cctcaaatag atgcgagaga
      121 ctgtggtgtc gctgccttag cctcgattgc taaattctat ggttcagatt tttctctagc
      181 tcacttgaga gaacttgcaa agaccaataa agaagggacg actgctcttg gcattgtaaa
      241 agccgctgat gaaatgggct ttgaaacaag gcctgttcaa gcagataaaa ctctctttga
      301 catgagtgat gtcccctatc catttatcgt tcatgttaac aaagaaggaa aactccaaca
      361 ttactatgtt gtctatcaaa caaagaaaga ctatctgatt attggtgatc ctgacccttc
      421 tgtaaaaatc actaaaatgt caaaagaacg ctttttctct gaatggactg gagtagctat
      481 ttttctagct cccaaaccca gctatcaacc ccataaagat aaaaagaatg gtctactaag
      541 cttccttcct ctgattttca aacaaaaatc tctcattgct tacattgttc tctcaagctt
      601 attggtcact attatcaata tcggtggttc ttattatctc caaggaatct tggatgaata
      661 catcccaaat cagatgaaat caactttagg aatcatctca gttggtctgg ttatcaccta
      721 tatcctccaa caagtcatga gcttttccag agattatctc ctaaccgttc tgagccagag
      781 attaagcatt gatgtgattt tatcctatat tcgccatatt tttgaacttc ccatgtcttt
      841 ctttgcgaca cgtcgtacag gagaaatcat ttcacggttc acagatgcta actctattat
      901 agatgccttg gcttctacca ttctttctct ttttctggat gtttctattc tgattcttgt
      961 agggggcgtc ttactggcac aaaaccctaa tctcttcctc ctttctcttc tttccattcc
     1021 tatatacatg ttcatcatct tttcttttat gaaacctttc gaaaaaatga accatgatgt
     1081 catgcaaagt aattctatgg ttagctctgc cattatcgaa gatatcaacg ggattgaaac
     1141 tataaagtcg ctcacgagtg aagaaaatcg ctatcaaaat atagacagcg aatttgtaga
     1201 ttatttggaa aaatccttta agctcagtaa atattctatt ttacaaacga gtttaaagca
     1261 gggaacaaaa ttagttctga atatccttat cctatggttt ggcgctcaat tagtcatgtc
     1321 gagtaaaatt tctatcggtc agctgattac ctttaacaca cttttttctt actttacaac
     1381 tcctatggaa aatattatca acctccaaac caaactccaa tctgcgaagg tcgctaataa
     1441 ccgtttgaac gaagtctatc tagtcgaatc tgaatttcaa gttcaagaaa accctgttca
     1501 ttcacatttt ttgatgggcg atattgaatt tgatgacctt tcttataagt atggttttgg
     1561 acgagatacc ttaacagata ttaatctcac gattaaacaa ggagataagg ttagcctagt
     1621 tggagttagt ggttctggta aaacaacttt agccaaaatg attgtcaatt tctttgaacc
     1681 ctacaaaggg catatttcca tcaattatca ggatattaaa aacattgata aaaaagtctt
     1741 gcgccgtcat attaattacc taccccaaca agcctatatc tttaatggct ctatcttgga
     1801 aaatttaacc ttgggcggta atcatatgat tagccaagaa gatattctaa gagcttgtga
     1861 attagctgaa atccgtcaag acattgaaag aatgcctatg ggctatcaaa ctcagctctc
     1921 tgatggagct ggtctatcag gaggacaaaa gcaacgaatc gctctcgctc gtgctctttt
     1981 aactaaagct cctgttttaa tactagatga agctactagc ggtcttgatg tcttgactga
     2041 gaaaaaggtt atagataatc ttatatccct aactgataaa accattctct ttgtagccca
     2101 tcgtctcagt atagccgaac gaactaaccg tgtcattgtt cttgaccagg ggaaaatcat
     2161 tgaagttggt agtcaccaag agttaatgca ggcgcaaggc ttctaccatc atctattcaa
     2221 taaataagga gaatgtcatg aatcctaatc tttttagaag tgtcgagttt tatcagagac
     2281 gttaccataa ctatgcgaca gtgttcatta tacctctttc attactattt actttcatct
     2341 tgattttctc ccttgttgcc acaaaagaaa ttactgttac ttcccaagga gaaatcgccc
     2401 ctacaagtgt cattgcatct attcagtcga ccagtgataa tcctatcctt gctaatcatt
     2461 tagtggcaaa tcaagtagtt gagaaaggtg acttactcat caaatactct gaaacaatgg
     2521 aagaaagtca gaaaactgcc ttagcaactc aattacaaag acttgagaag caaaaagaag
     2581 gacttggaat cttgaaacaa agcttagaaa aagcgactga tcttttttct agcgaggatg
     2641 agtttggcta ccataatacc tttatgaatt ttactaaaca atcccatgat attgaactgg
     2701 gtatctcaaa gactaacact gaagtttcaa atcaagctaa tcttaccaat agcagttcat
     2761 cagccatcga acaagaaatt acaaaagttc aacaacaaat tggagaatat caagagttga
     2821 gagatgctat cataaataac agagcacgct taccaactgg caatccgcac cagtcaattt
     2881 taaatcgtta tcttatagcc tctcaaggac aaacacaagg aacggcagag gagccatttt
     2941 tatctcaaat taatcaaagt attgcaggtc tggaatcatc tatcgcaagc ctcaaaattc
     3001 agcaagctgg tatcggaagt gtagcaactt atgataatag tttagcaacc aaaattgaag
     3061 tactccgcac tcagttttta caaacagcct cacagcaaca actaaccgtg gagaatcaat
     3121 taacagaatt aaaagtacaa ctagatcaag cgacacagcg cttagaaaat aataccttaa
     3181 ccgctccaag taaaggtatc gttcatctga acagcgaatt tgaaggtaaa aatagaattc
     3241 caactggtac agaaattgct caaatattcc ctgtcatcac agatacaaga gaagtactaa
     3301 tcacttacta cgtatcatct gactatctcc ccttactaga taaaggacaa actgtaagat
     3361 taaaactgga gaagattgga aatcacggca ccaccatcat cggccaactt cagagtattg
     3421 atcaaactcc taccagaaca gagcaaggca atctctttaa attaaccgct cttgcaaaac
     3481 tatctaacga ggatagtaaa ctcatccaat atggcttaca aggtcgcgtc actagtgtaa
     3541 ctacaaagaa aacatatttt gattatttca aagataaaat tttaacccat tctgattaat
     3601 tttcagataa cactctataa ctatttatta tcttatcaaa aaggagaatc ataacatgga
     3661 taagaaacaa aacctaactt catttcaaga actaacaact accgaactca accaaattat
     3721 aggtggagga tggtgggaag atttcttata tagatttaat ataattgaac aaaaaaatac
     3781 aaaaggattt tatcagccaa tacaactata aaaaaacaag accaagagaa cgcactctcg
     3841 gtcttatttt ttcttcattc tatatgtatt acagtaagta cctgacgaaa aacgtgatct
     3901 tggcaggtgg tattgagact ggtattggga tgactttcca caattttcat aacggtatag
     3961 agaccaactc ctctctcctc ccctttagaa cttgctccaa aggagaagat ttcagaaata
     4021 tcgattccct cttctttgat ggaattttca atgataaagg tctcctgtgc tccattttta
     4081 aaaaaggcaa ttgaaacatg aggttgacag gcttctacac tagcttcaat agcgttgtca
     4141 caaaggatag acacaacggt tagaaaatca agtagactca ccccctctac ctgaatctcc
     4201 tctggaactt cgacattaaa gacaatgttc ttatctctag cttttataaa ttttccagct
     4261 aggagacttt tgagggcacg atcccgaaca ttcaccaatc ggccaaggtc atatttattg
     4321 tcctgcaatt tttcactaga atcctttaag accgagtcgt agatctcttt tatctgctcc
     4381 atatcctcct cttcaatgcc cagacgtaag ctagtcaaga ggttggtgta gtcatggcga
     4441 aaactccgta cttccttata aagctcctct atatgccgac tataccgctc catctctctg
     4501 tagcgcaagt cctgctcttg gttcagtctc tcatggagtt tgtccttcaa ataggtatcc
     4561 aatttcttga taatccccat aaaaaagagc aggtaaaaga ctaggatgag atggcgaaca
     4621 gtcgttgatt gaatcccctg ttcatattca aagtaagaca gattttgtat caccaaatag
     4681 taagccccca ttatccagtt aatctgagtc agggactttt gaaaatcttt atcgagaatc
     4741 ccctttctca agctagtgaa atcatagtcc aaccatttca aaaaagctag agaaatgaag
     4801 aaattgaaaa ttattataca taaccaaata aatgagtagt catcatatac ttgcccttgt
     4861 cccaaaaatg gaagcacaaa ataggaaaca cctctataaa agagattcac taatatcatt
     4921 ggaaagagac cataaaagag aaggagtttt ttaggaagtt ctcttaacaa taagaaagat
     4981 aagcctatgc cgtacaaggg ttccataaaa taagataggt aaacatttcc taccatatag
     5041 ccaagcattc caaaaacaac ggccaacagt atcttaaaaa gaaaggcctt aaaaatcctc
     5101 tcaaaagtga gactaattcc atctacctta aagaatatga caatttttaa tccgtgagta
     5161 acaagtgtat acaacaatat ccaagcaaca ttcataaact ctcctagctc agtgtaagtt
     5221 attaatagcc tcagacactt ccctgacctt ataacgcgcg attagacaac ttccaccatt
     5281 gggaaagaaa agcagttttt ctttcttatc taaatgcacc acattggcag gattgatgag
     5341 aaaagagcga tggcactgca agaaacgagg ctcctgcttg agaacctcct ctagactcgc
     5401 tgtaaattcc agcctgtctg tcttggtata gagaataaca cgatggggtc ttgtggacgt
     5461 ttcgagatag taaacctctt taaaaggata ctggaattga gcaaattttg atttaaagta
     5521 aaagcaatct tccgctagac ttttactgtc ttgaccattg gcatagagga gggctgtctc
     5581 gatacgagat tcaaactcct ctgccgaaag ggccttatca atataatcca gagcagacac
     5641 ttggtagcga aaggacaggg gcataaactc cgagtgagtc gtcacaaaga caatcagggc
     5701 atacggatcc cgatcccgaa tctttctagc cacttccaga cccttcatct cctcatttcg
     5761 aatctcaata tccaaaaaga atagctgatg ggcccccttc tcatgcacct ctgccagcaa
     5821 ttgatctggc ttaccaaaaa cttcaaaaga actcagagtg atatgatgtt ccttcaagag
     5881 tttctcaatc gtcgtttcaa ttctagtctg ttgggaaaaa tcatcttcta aaacaaatat
     5941 tctcatcttc ttactctcct tctttcaacc atttttgacg aatttctcta tagcgacgtc
     6001 tggcatactt gacagcgtat tgaccttctt ctccaagaaa aaggattctt tcttgaaaat
     6061 caatcgattt tactttatca agattgacca aacaatttcg atgacatctc atcaaggttt
     6121 ccccatattg gttctcaaga ttacttaaat tttgaaccat attaaaacta gcctcttctg
     6181 taacaatctt tacagtatgg gctttagttg gatgtgtttg gatatagtag acatcgtcga
     6241 cgtttacttt atagactgtt ttctgtgtct gaataatcat atgtttcatt ggattctcct
     6301 cctaagaaaa gaataacaga aaaaatctaa cacaagcaca cccaatccta aacagtcttt
     6361 gaaagctcct aaaagaatta tttttctaca atatcatgat gagcttacca ctattttatc
     6421 ataaaattca ctcagcgcaa ttcaagacat ttcaatgaca attaaagatt taaaggcgaa
     6481 aaaggagata aaagtgatag actgacagta tcaaaacaca gttgctagaa tcaagactgg
     6541 cacccagatt agaggaggaa ttctcatgac agatacagac cctatcaaaa gagctcagac
     6601 tttgattact gacttaaaca aagcctatca agcatgcaaa caggcaaccg ctgacgacgt
     6661 ccgctttcag gagcaattaa actctattct tggttttcta gccaaggctg aaacagtgga
     6721 taatcgattc ttgattgaat tggaaaaatt ttaccagact tccagtcttc tcatgggact
     6781 cagcgctctt gatccagatg ctccaactcg cgccgcttgg cgggcctatg accgcttcca
     6841 ctttgaccaa gtcaagacca agttaatact caatgagaat caaagagcaa actaggaagc
     6901 tagccgcaag ctgtacttga gtacggtaag acgacgctga cgtggtttga agagattttc
     6961 gaagagtata agtctctacg gaccaaccat tatcctgtag taacaaaaaa gaagttgaga
     7021 cagactgaac tcaacttctt ttttagtgtc gtatcagcaa tgttagtcct gcatctgacg
     7081 ttttacctga taaggcaaat acaagactag taaaaccaaa ccagctccta gtaaggctag
     7141 aagggctagt ttttcttgga aggtaatcaa cccaacaact acttccacaa gtaaaacaaa
     7201 actggtcaat cctcgaacta cctcctgtaa gcctttttcc ttttgcccct tgtccagcgg
     7261 aaagagttgg gtcaaatact ggtagtcaaa ggcatgatag agggccagca actggaagag
     7321 caagaggtag ttaaagagaa ctaccactgc tgtcgcaatc caagcttgct cgataaaaac
     7381 ctgcgccagt acggaaagta agagaagacg aagactgaga gcaaagaggt cgccatttcg
     7441 cagataagaa cgcagataga gattttgcca aatcttccca ggcaccttct gaacagcctt
     7501 taagataaag tccagatagg cacgacgatt gacactgttt gaaattccct tgacctgcgt
     7561 aaagagggca aagaaacgaa gcaagacttg cttacgcttg ctttcttgag aaataagata
     7621 gtcccagccc agtccagttt cagtgaaaaa tttgctggcc ttttgacgaa agtggaaata
     7681 ttttcctacc cccaataaaa gcacatagag cagaaaaact ggcaagccat aacccattgc
     7741 taaaaacaag ggcgcaaata acagcaagaa aagggtctgt acaaagagcc aaaagactag
     7801 ggaaatgcca gtttgacgct tgagatggag cttgatttcc tcttctccaa ctaagagaaa
     7861 gagcttgtct ggagcctcca tataggtggc aattcctccc caaagtaaaa gtaaaacaga
     7921 cgtaattcct acaaacaaaa ggataggcca atgattttca ggaaaatgtt gtaagagttg
     7981 actgtactgg taggctagaa agcccaacag gacaagcagg aacaagacaa agtggtcatt
     8041 gagcacatag cgcagataac caagacactc cttacgaaag gcctgctttc tctttaaaaa
     8101 caagtctttc atagatcctc ctctttggtc agagccaagt aaatatcatt caaactagcc
     8161 tcaggcatat caaaggcttc acgtagttgc aggagattgc ctttggcacg cacctctccc
     8221 ttgtgaagaa tgacaaaggc atcacacatc ttctccgccg aatccagcac gtgggtactc
     8281 atgagaatag acttgccctt ttgcttctcc acttccaaaa gctgaatcaa atcagaaata
     8341 gccagcggat caagaccaag gaaaggctca tccacgatga aaagacttgg atccaccaca
     8401 aaagcacaga taatcatgac cttctgcttc atcccttttg aaaaatgaac agggaaccag
     8461 tctaatttct gttccaaacg gaacattttt aacaagggct ctactcgttc gaaagccact
     8521 ttttgctcaa taccgtaagc catagcaacc gtttcgatat gctctctgag ggtcaattcc
     8581 tcatacagac taggcgtctc aggaatgtag ccaatctgct tgcggtagct agtcgcatct
     8641 ccttgcagag tcaggccatt gatattgatg gagccactat aaggtgccaa cagaccgata
     8701 atctcattga tcgtcgttga tttcccagca ccattgagac caatcaaacc gaccaactgc
     8761 ccactttcaa cagtaaagga cacatctttc aaaacaggaa catgaacata gccacctgtc
     8821 aggtttttaa tttctaacat attttctcca aatctggtat aatgtagcta tattatatca
     8881 aaattcagta cagtagaggt agattttatg tcagattgca ttttttgtaa aatcatcgca
     8941 ggggaaattc ctgcttcaaa agtatatgaa gatgagcagg tccttgcctt tcttgatatc
     9001 tctcaagtaa cactaggaca caccttggtc gtgccaaaag aacactatcg caatcttttg
     9061 gagatggatg ctacgagcgc cagccaactc tttgcccaag taccaaaagt agctcaaaaa
     9121 gtcatgaaag tcactaaggc tgctggtatg aatatcattt ccaactgtga agaagtcgct
     9181 ggtcaaacag tttttcatac tcacgttcac cttgtgcctc gctacagtgc tgacgatgac
     9241 ctcaagattg attttatcgc ccacgaacca gactttgaca aacttgctca agtcgctgaa
     9301 accatcaaaa acgcttaagg agttgctatg aaattatcca acctactgct atttgcagga
     9361 gctgcagccg gaagttatct ggttacaaaa aatcgccaaa ccatcacaga tgaagtcttg
     9421 aataccactg accgcgttca agctatcaag gacgatgtgg atattatcca aaacagcctg
     9481 caaatcatta accagcaaaa agaacttatc aaggaatacc aagaagactt gacttacaag
     9541 tttaaggtct tggaaaagga tatccagact agactagctg tgataaaaga aatgcaggga
     9601 actgaagata agtaaaaaga gcccgacggc tctttttt
//
LOCUS       DAVMHG010000072         9455 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMHG010000072 DAVMHG010000000
VERSION     DAVMHG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.072"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            12..1940
                     /gene="glgB"
                     /locus_tag="VYR08_001362"
     CDS             12..1940
                     /gene="glgB"
                     /locus_tag="VYR08_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV3466880.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            1930..3072
                     /locus_tag="VYR08_001363"
     CDS             1930..3072
                     /locus_tag="VYR08_001363"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV3466881.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3062..4201
                     /gene="glgD"
                     /locus_tag="VYR08_001364"
     CDS             3062..4201
                     /gene="glgD"
                     /locus_tag="VYR08_001364"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV3466882.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKSAKVTEDIHS"
     gene            4198..5631
                     /gene="glgA"
                     /locus_tag="VYR08_001365"
     CDS             4198..5631
                     /gene="glgA"
                     /locus_tag="VYR08_001365"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV3466883.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGFPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5690..6338
                     /gene="serB"
                     /locus_tag="VYR08_001366"
                     /pseudo
     CDS             5690..6338
                     /gene="serB"
                     /locus_tag="VYR08_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            6347..7462
                     /locus_tag="VYR08_001367"
     CDS             6347..7462
                     /locus_tag="VYR08_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV3466884.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTYGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLDLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(7459..7905)
                     /locus_tag="VYR08_001368"
     CDS             complement(7459..7905)
                     /locus_tag="VYR08_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV3466885.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8069..9373
                     /gene="eno"
                     /locus_tag="VYR08_001369"
     CDS             8069..9373
                     /gene="eno"
                     /locus_tag="VYR08_001369"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV3466886.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
BASE COUNT         2811 a         1700 c         2108 g         2836 t
ORIGIN      
        1 aaagggagta tatggataat agagaagcat taaaaacctt tatgacgggt gaaaattttt
       61 atctccaaca ttatctagga gcacataggg aagaactaaa tggagagtat ggctatacct
      121 tccgtgtttg ggcacctaat gctcaggctg ttcacttggt tggtgatttt accaattgga
      181 ttgaaaatca gattccaatg gtaagaaatg actttggggt ctgggaagtc tttaccaata
      241 tggctcaaga agggcatatt tacaaatatc atgtcacacg tcaaaatggt catcaactga
      301 tgaagattga cccttttgct gtcaggtatg aggctcgtcc aggaacaggg gcaatcgtaa
      361 cagagcttcc tgataagaaa tggagggatg gactttggct ggcacgaaga aaacgttggg
      421 gctttgctga acgtcctgtc aatatttacg aggttcatgc tggctcttgg aagagaaatc
      481 ctgatggcag tccttatagt tttgcccaat tgaaagatga actcattcct tacctagttg
      541 agatgaacta tactcatatt gagtttatgc ccttgatgtc ccatcctttg ggcttgagtt
      601 gggggtatca gcttatgggt tacttcgctt tagagcatgc ttatggccga ccagaggagt
      661 ttcaagattt tgtcgaggag tgtcataccc ataatattgg ggttattgtg gactgggtac
      721 caggtcactt taccatcaac gatgatgcct tagcctatta tgatgggaca ccgacttttg
      781 aataccaaga ccataataag gctcataacc atggttgggg tgcccttaat tttgaccttg
      841 gaaaaaatga agtccagtcc ttcttaattt cttgcattaa gcattggatt gatgtctatc
      901 atttggatgg tattcgtgtg gatgctgtta gcaacatgct ctatttggac tatgatgatg
      961 ctccatggac acctaataaa gatggcggaa atctcaacta tgaaggttat tatttccttc
     1021 agcgcttgaa tgaggttatt aagttagaat atccagatgt gatgatgatt gcagaagaaa
     1081 gttcgtctgc gaccaagatt acgggaatga aagagattgg tggtctagga tttgactaca
     1141 aatggaacat gggctggatg aatgatatcc tccgtttcta cgaagaagat ccgatctatc
     1201 gtaaatatga ctttaacctg gtgactttca gctttatgta tgttttcaag gagaattatc
     1261 tcttgccatt ctcgcacgat gaagtggttc atggcaagaa gagtatgatg cataagatgt
     1321 ggggagatcg ttacaatcaa tttgcaggct tgcgcaatct ctatacgtac caaatttgtc
     1381 accctggtaa gaaattgctc ttcatgggta gcgaatacgg tcaattccta gaatggaaat
     1441 ctgaagaaca gttggaatgg tctaacctag aagacccaat gaatgctaag atgaagtatt
     1501 tcgcttctca gctaaaccag ttttacaaag atcatcgctg tctgtgggaa attgatacca
     1561 gctatgatgg tattgaaatc attgatgcgg ataatcgaga ccagagtgtt ctttccttta
     1621 ttcgtaaggg taaaaaggga gaaatgttag tctgtatctt taatatggta cctgttgagc
     1681 ggaaagattt tacaatcgga ctacccgttg caggaattta cgaagaagta tggaatactg
     1741 agttggaaga gtggggaggc gtttggaaag aacataatca aactgttcaa acgcaagaag
     1801 gactatggaa agattatgag cagaccttaa cctttaccct accggctatg ggagcaagtg
     1861 tatggaaaat caaacgtcgc ttgaaatcta ctaaaaccgt cacaaataaa aaccaaaaag
     1921 gagtagaaaa tgaaaaatga aatgttagct ttgattcttg ctggtgggca aggaactcgt
     1981 ctcggtaaac tcactcaaag catcgcaaaa ccagctgtgc aatttggtgg gcgctaccgt
     2041 atcattgact ttgccctatc aaactgtgcc aactcaggga ttcacaatgt tggggtcgtt
     2101 acacagtatc aaccacttgc tctcaacaac catattggga atggttcaag ctggggacta
     2161 gacggtatta attcaggtgt ctctattctt caaccttatt ctgcaagtga aggaaatcgt
     2221 tggtttgagg ggactagtca cgctatttac caaaatatcg actatatcga cagtgtcaat
     2281 cctgagtatg tcttgatttt gtctggggac cacatctaca aaatggacta tgatgatatg
     2341 ctccagtctc ataaggataa taatgccagc ttgacagtag cagttttaga cgtccctctt
     2401 aaagaagcaa gccgttttgg tatcatgaac acagatgcta acaatcgtat tgttgaattt
     2461 gaagaaaaac cagctcaacc taaatctaca aaagcttcta tgggaatcta catttttgat
     2521 tggcaacgcc ttcgtaatat gttagtcgct gctgaaaaga gcaaggttgg catgtcagac
     2581 tttggtaaaa atgtcattcc aaattacctt gagtcaggtg aaagtgttta tgcttacgaa
     2641 tttagtggtt attggaaaga tgttggtact attgagtcac tttgggaagc gaacatggag
     2701 tatatttctc cagaaaatgc cttggatagt cgtaaccgtc aatggaagat ttactcaaga
     2761 aacttgattt caccaccaaa cttcctcggg gcaaatgctc atgtggaaga ctcattagtt
     2821 gtagacggat gtttcgttga tggaactgtt aaacattcta tcctttcaac aggcgcgcaa
     2881 gttcgcgaag gagcggaagt ccttgattca gttatcatga gtggagctat cattggtcaa
     2941 ggagctaaga ttaaacgtgc cattattggt gaaggtgcga ttatttctga cggtgtcgaa
     3001 attgatggaa cagatgaagt acaagttgta ggatataatg aagtagtggg ggtagcaaca
     3061 gatgaagatt gataaatatt ctgccatttt aggaaataca gttggttttc acaatatgtc
     3121 gacattgacg gaccaccgtc cagtagcaag tttgccattt ggtgggaaat atcgtttgat
     3181 tgacttccca ctttcaagcc ttgctaatgc aggtgttcgt agtgtctttg gtattttcca
     3241 gcaggataat atcagctcag tatttgacca tattcgttca ggacgcgagt ggggcttatc
     3301 aacccttctt agccattact atctaggaat ttacaatacc cgtgtagaaa gtagtacagt
     3361 tggaaaagaa tactaccaac agcttcttac ttatttgaaa cgttctggct caaaccaaac
     3421 ggttgccctc aactgcgatg ttcttattaa cattgatttg aaccaagttt tccacctaca
     3481 tagtacaaca aaagagccta tcactgtagt ttataaaaaa ctagctaaga aagatatttc
     3541 agaagtaaat gcaatcttgg atgtagatga aacagaccat gttctttctc ataaactctt
     3601 tgatagcaag tcaacagctg aaacgttcaa tatgtctaca gatatctttg tcgttgatac
     3661 accttggttg attgaacact tggaagaaga agctaaaaaa gaacatccag agaaattgcg
     3721 ctatgtttta cgggatttgg ctgtaaaaga gggagctttc gcctacgagt acacgggcta
     3781 tctagccaat attcactctg ttaaatctta ttatcaagcg aatatcgata tgcttgaatc
     3841 acaaaaattc tattctcttt tctcaccaaa ccaaaagatt tatacaaagg tcaaaaacga
     3901 agagccaact tactatgcta atacatctaa ggtaagcact tctcagtttg cctctggtag
     3961 tattattgaa ggtcaagtag ctaattctgt tctatcacgt aatattcatg ttcataagga
     4021 tagcttggtt aaagatagcc tgctcttccc tcgtgttgtt attggagaag gggctcaggt
     4081 cgaatatgct atcttggaca aaggggttga agttgagcct ggtgttgtga ttcgaggaac
     4141 tgcagaacat ccagttgtgg ttaagaaaag tgctaaagta acagaggata ttcattcatg
     4201 aaaattttat ttgtagcagc agagggtgca cccttttcaa aaacaggtgg tttgggagac
     4261 gtcattggcg ctcttccaaa atcactggta aaagctgggc acgaagttgc agtgatttta
     4321 ccctactatg atatggtaga ggctaaattt ggaaatcaga ttgaagatgt gcttcatttt
     4381 gaggtgagcg ttggttggcg cagacagtat tgtggaatta agaaaacagt attaaatggt
     4441 gtaaccttct actttattga caatcaatat tatttcttcc gtggtcatgt ttacggtgat
     4501 tttgatgacg gagaacgctt tgcctttttc caactggctg ccattgaggc tatggaaagg
     4561 attgacttta ttcctgatct tctccatgtt catgactacc atacagctat gattcctttc
     4621 ttgttgaagg aaaaataccg ttggattcaa gcctatgagg acattgaaac agttttaacc
     4681 attcataatt tagaattcca aggacaattt tcagaaggaa tgttgggtga tttgtttgga
     4741 gttggctttg aacgttacgc tgatggcacc cttcgatgga acaactgtct gaactggatg
     4801 aaggcaggta ttctctatgc gaaccgtgtt tcaaccgttt cacctagcta tgctcatgaa
     4861 attatgacta gtcagtttgg atgtaatttg gatcagattc ttaaaatgga gtctggtaaa
     4921 gtatctggta tcgtgaatgg gattgatgct gatctttata atcctcagac ggatgctctt
     4981 ttagactatc atttcaatca ggaagatttg tctgggaaag ccaaaaataa ggcaaaattg
     5041 caagaaagag ttggctttcc tgttagagct gacgttccac tggtgggaat tgtttctcgt
     5101 ttgacacgtc aaaaaggttt tgatgtggtg gtcgaaagtc ttcaccatat cttgcaagaa
     5161 gatgttcaga ttgttctttt gggaactggc gatccagcct ttgaaggagc tttctcatgg
     5221 tttgctcaga tttacccaga caagctatca gcaaatatca cttttgatgt caaacttgct
     5281 caggaaatct acgctgcttg tgacctcttc ctcatgccaa gtcgttttga accgtgtggc
     5341 ttgtctcaaa tgatggctat gcgttatgga accttgccat tggtccatga agttggaggc
     5401 ttgcgagata cagttcgcgc tttcaatcca atcgaaggaa gcggtactgg ctttagcttt
     5461 gacaatctat ctccttattg gttaaattgg actttccaaa cagcattgga cttgtataga
     5521 aaccatccag acatttggag aaacctacaa aaacaagcta tggagagtga cttctcatgg
     5581 gatacagcct gcaagtcata ccttgacttg taccatagtt tagttaatta atagataaaa
     5641 tcgtatgatg acttcatacg atttttaggc tgtttagaga ggagaactga tgtctcaagt
     5701 aaaaggcttg tgtgttttgg atgtagatgg aaccttaatc ctagaagaag tgattgatct
     5761 tttagggaga gaggcaggtc atgaggcgga aatttcgcag attacaagtc gggcaatgcg
     5821 aggagagttg gtctttgaaa gcagtttaag aaaaagagtg tccttgttgg aaggtcttcc
     5881 tattttggtc tttgataacg tcttcaactc aattcatcta tcgctaaatg tcccagagtt
     5941 catctctatt ctccaaaaga atggcatcct agttggtctg gtgtgtggtg tctggtggat
     6001 accaatagtt ggagagatta gcaaaatccc ttggtattgc ctatttcact gccaaccagc
     6061 ttgaagtcaa agaaggtctt ttaacaggaa aattagttga acaaattata agtccccagg
     6121 tcaaaaaaga gactctggaa aaatggagaa agaaactaaa actttctaaa gaaagaacgg
     6181 tggcaatcgg tgatggggtc aataatctat taatgttgaa gtcggcggag ttaggaatcg
     6241 ccttttgtgc caaagaagtg ctcaaaaaag aaataccaca tcatgttgac aagagggatt
     6301 ttttagaagt tcttcctttg attgactgtt tagaatgagg gaaaatatga agattgtaat
     6361 tgcaccagat tcgtttaagg aaagcttgac agctcaacag gtagctgaag caataaaaag
     6421 aggcttccaa caatcgatag cagatgtaga atatctcctc tgccctgttg gtgatggggg
     6481 agaaggtact gtagatgcta tccgacattc tcttgaccta aaagaaaaat ggatccaagt
     6541 gacagaccct tttggacaaa aagaagccat gcgctatttt caaaaagggg aactggcact
     6601 atttgaagta gctgacttgg ttggtcttgg aaaaattccg ctagagaaac gaaatccact
     6661 tcaaatccaa acttatggta ttggagagtt gattctccat ctcatttcta aagggattaa
     6721 agatatttat atcggtgttg gtggcacggc cagtaatgat ggaggactgg ggattgctgc
     6781 tggtttaggt tatcaatttt atgataggga tggaaatgtc ttgcctgctt ccggtcaatc
     6841 cttattagac ttagcttctg tttcaacaga aaattgctat aaaattcctg aaggtgttca
     6901 aattcatatt ctagcagatg tcgtgagtcc cttatgtggt catcaaggtg cgacttacac
     6961 ttttggcaat caaaaaggtc tacatccgac tatgtttgca gtcgtagatc aggcgatcca
     7021 agatttttat gaaaaattct cacctgcaac attagaaatt aaaggagcag gagcaggtgg
     7081 aggccttgct ggtggtttgt gtgcctttgc tcaggcaagt atcgtgtctg gaattgatac
     7141 ctgcttggac ttaatcaact ttgataagaa agtttcagat gctgacttgg ttgttgttgg
     7201 agaaggaaga cttgatagtc aaagctttgc tgggaaagcg cctataggtg ttgcaaaaag
     7261 aacccctgtt ggagttcctg ttattgctat ttgcggtagt cttgctgaag atttacctcc
     7321 cctaccattt gaaaatatcc aagctgtctt ttctattttg gagaaaagtg aacctttaga
     7381 agatagtttg aaaaatgcca gtctctatct ggagcgcacg gctgccaata ttgggcactt
     7441 attaaatatg tgcaagattt agctaaacca tttcttccat aaggatgttt tggctggttc
     7501 ttccttttta acttcccaaa gttttggaaa agcaagtcga aggtcttttt cttctacttg
     7561 aactggtgca ttgctatgaa taaccaggcc aaaaggagaa gtaatatgct cttcagatac
     7621 tatggtggct tgactatcag tttcttttgc ttcttttaag tagaaaactt gcttatcaaa
     7681 ttcgatagtt ggtgaaatct tcacaaatag tggctctgct ttttcctgaa ggttttctaa
     7741 aataaataaa aagccttttt ctaactgagg gctatttgct gtgtcaatat ctacatatcc
     7801 aagaactctt tcctcaaaag taccaagata gcgtctttgc tcatccggat ttatttttaa
     7861 cccaccatga gctttttcaa gtaattgttt tgataaatct gtcatgaaaa cagtatatca
     7921 taaaagttag aataaaacag acaaaagaaa gcgattactt tataaagtta aggaaaagtt
     7981 gcacaaagat aacgtttttt cttgaaaaag gaggggtttt aaggtattat agaggtgtaa
     8041 aaaatttaac tcataaggag agtaaaaaat gtcaattatt actgatgttt acgctcgcga
     8101 agtcctagac tcacgcggta acccaacact tgaagtagaa gtttacactg aatcaggtgc
     8161 tttcggacgt ggtatggttc catcaggagc ttctactggt gaacacgaag cagttgaact
     8221 tcgcgacggt gacaaatctc gttacggtgg tcttggtaca caaaaagctg ttgacaacgt
     8281 aaacaacatc attgctgaag ctatcattgg ctacgatgta cgtgatcaac aagctattga
     8341 ccgtgctatg atcgcacttg acggtactcc taacaaaggt aaattgggtg cgaatgcaat
     8401 cctcggtgtg tctatcgctg tagctcgtgc tgctgctgac taccttgaaa tcccacttta
     8461 cagctacctt ggtggattca acactaaagt tcttccaact ccaatgatga acatcatcaa
     8521 cggtggttct cactctgacg ctccaatcgc tttccaagag ttcatgatct tgccagttgg
     8581 tgcgccaaca tttaaagaag cccttcgtta cggtgctgaa atcttccacg ctcttaagaa
     8641 aatccttaaa tcacgtggtt tggaaactgc cgtaggtgac gaaggtggat tcgctcctcg
     8701 tttcgaagga actgaagatg gtgttgaaac tatccttgct gcgattgaag ctgctggata
     8761 tgtaccaggt aaagacgtat ttattggatt tgactgtgct tcatcagaat tctacgataa
     8821 agaacgtaaa gtttacgact acactaaatt tgaaggtgaa ggtgctgctg ttcgtacatc
     8881 tgcagaacaa atcgactacc ttgaagaatt ggttaacaaa tacccaatca tcactattga
     8941 agatggtatg gatgaaaacg actgggatgg ttggaaagct cttactgaac gtcttggtaa
     9001 gaaagtacaa cttgttggtg acgacttctt cgtaacaaac actgactacc ttgcacgtgg
     9061 tatccaagaa ggtgctgcta actcaatcct tatcaaagtt aaccaaatcg gtactcttac
     9121 tgaaactttt gaagctatcg aaatggctaa agaagctggt tacactgctg ttgtatcaca
     9181 ccgttcaggt gaaactgaag attcaacaat cgctgatatc gcagttgcaa ctaacgcagg
     9241 acaaatcaag actggttcac tttcacgtac agaccgcatc gctaaataca accaattgct
     9301 tcgtatcgaa gaccaacttg gtgaagtagc tgaatatcgt ggattgaaat cattctacaa
     9361 ccttaaaaaa taaaatagtt cagtgaacta ttttatcccg aaccatgaaa ttcaaaagtt
     9421 cggccgttga tttaacaacg tttctagccc ctcgg
//
LOCUS       DAVMHG010000073         9452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMHG010000073 DAVMHG010000000
VERSION     DAVMHG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.073"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(286..2109)
                     /gene="dnaK"
                     /locus_tag="VYR08_001370"
     CDS             complement(286..2109)
                     /gene="dnaK"
                     /locus_tag="VYR08_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV3466887.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2589..3113)
                     /gene="grpE"
                     /locus_tag="VYR08_001371"
     CDS             complement(2589..3113)
                     /gene="grpE"
                     /locus_tag="VYR08_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV3466888.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3140..4174)
                     /gene="hrcA"
                     /locus_tag="VYR08_001372"
     CDS             complement(3140..4174)
                     /gene="hrcA"
                     /locus_tag="VYR08_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV3466889.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGFLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4338..4847)
                     /locus_tag="VYR08_001373"
     CDS             complement(4338..4847)
                     /locus_tag="VYR08_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV3466890.1"
                     /translation="MNSRVEFRIFTILDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQEFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREELSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            5454..7787
                     /locus_tag="VYR08_001374"
     CDS             5454..7787
                     /locus_tag="VYR08_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3466891.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7800..9263
                     /locus_tag="VYR08_001375"
     CDS             7800..9263
                     /locus_tag="VYR08_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3466892.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCDFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9263..>9452
                     /locus_tag="VYR08_001376"
     CDS             9263..>9452
                     /locus_tag="VYR08_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEV3466893.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         2924 a         1776 c         1846 g         2906 t
ORIGIN      
        1 cggtattaca aatttatatg tttgtatttc taatgatatc atagctggta ccatcgccga
       61 tacaaatgca gacaacaata ctgaggtaac aaaattcctt accgcttcat catttttgat
      121 acaactatca tgttcagcta atataattct ctatatctag ctaaatattc tattggaata
      181 ttatcaataa aatcttttga tgttctatag ttatcagctt gattacaaaa atcattataa
      241 acttttcgtg tattttttag atactcttca tccaatacac tcatcttact tttccgtaaa
      301 ctctccgtct acgacgtcat cgcctgcgtt ccctgttgct tgtgcgcctt ctgctccttc
      361 ttgagcttgt tgcgctgctg cggcttgttc gtagagttta acagcaagtc cttgagcttt
      421 ttcgttcaat gcttcaagtt ttgttttcat gtcgtccaag ttgttgtctt cttgagcttt
      481 cttaaggtca tcaagggcag cttgggcagc gtcacgttct gcgtcgaagc ctttaccttc
      541 agtttccttg attgtctttt cagtcgcaaa gattgcttgg tctacttcat tacgaaggtc
      601 tacttcttct ttacgtttct tatcggcttc agcgtttgct tctgcatctt tcatcatgcg
      661 gtcgatttct tcgtcagtca aacctgagtt cgattggatg acaatagttt gttctttttg
      721 agttccaagg tctttggcct taacagacac gataccgttc ttgtcgatgt caaatgttac
      781 ttcgatttga ggaattccac gaggtgcagc tgggatatca gtcaattgga agcgtccaag
      841 agtcttgtta tctgctgcca ttgggcgttc accttgaaga acgtggatat caacggctgg
      901 ttggttgtct gctgctgttg agaagacttg tgatttagat gttgggattg tagtgttgcg
      961 atcgataagt tttgtaaata ctccacccat tgtttcgata ccaagtgaca atggcgttac
     1021 atcaagaagg acaacgtcct tgacatcacc agtaatcaca ccaccttgga tagccgcacc
     1081 catagcaact acttcatcag ggtttactga tttgtttggt tctttaccag tttcagcttt
     1141 aacagcttca acaacggcag ggatacgagt tgaaccacca acaaggataa cttcgtcgat
     1201 ttctgacaag ctcaaacctg catctgaaag ggcttgacga actggaactt ttgtacgttc
     1261 aacaaggtca cgagtcaaat cgtcaaattt cgcacgagtc aaagtcattt ccaagtgaag
     1321 aggtccagcc tcacctgcag tgataaatgg caagctaatt tgtgttgaag ttacaccaga
     1381 aaggtctttc ttcgcttttt cagccgcatc tttcaaacgt tgcattgcca tcttgtcagt
     1441 agacaagtcg ataccgtttt ctttcttgaa ttctgctacc aagtggtcaa tgattttttg
     1501 gtcaaagtcg tcaccaccaa gtttgttgtc ccctgcagtt gacaatacgt cgaagacacc
     1561 gtcacccaat tcaaggatag agacgtcgaa tgtaccacca ccaaggtcaa ataccaagat
     1621 tttttcttct ttgtcagtct tgtccaaacc ataagcaaga gctgctgcag ttggttcgtt
     1681 aacaatacgt tctacttcaa gaccagcaat tttaccagcg tcttttgttg cttgacgttg
     1741 agcgtcgttg aagtaagccg gaactgtgat aacagctttg gttactttct caccaaggta
     1801 gtcttcagcg tagcctttca agtattgaag gatcatagct gagatttctt gtggagtgta
     1861 ttcttttcca tttgcagaaa ctttttcaga agttcccatc ttagatttga tagagataac
     1921 tgtatctggg tttgtaactg cttgacgttt tgcagcatca ccaacgatga tttctccgtt
     1981 tttgaatgag actacagatg gagttgtgcg gtttccttct gggtttgcga tgattttgct
     2041 ttcagttcct tcaagaactg caactgctga gtttgttgta cctaagtcaa taccgataat
     2101 tttagacatg tgtttttctc cttgttagtt aattttctat ttttcttttt gatactcttc
     2161 ttaagtggcg acgccgtcag agcttgactt cgtcaatctc tttgactaaa ctttgagcct
     2221 aaggtctcaa aatttgcgca aatagcgcca cggcgaagag tatcgtgttt ggttcgcttc
     2281 gctcactatt acaaagccaa acatatttta tctttcttca tagtttattg tcgtttcgga
     2341 caagttttct attatgtaaa ttgcgacacg aggagtcgaa atcgatttta tttcgacgac
     2401 gagttagtaa ggaagctagg caaacgccat agcgattgcc gttttctgac gagttgcttt
     2461 agctaccgtc agaattgccg aatcgaacac gccctaagct ctgagtaaaa aagatagatt
     2521 ttcttgtgtt catcgaacac ttcgtcaatc tcctattttt actgcgagct ttttacgggc
     2581 tttatatctt agttatacac cactaccatt gctgggcgta ggatgcggtc atggagtttg
     2641 tagccttttt gaaagacttg agcgatggta tctactgggt gttcatcgtc tgctgggaga
     2701 gtttggatgg ccatatggta gttatggtca aattcgccat ctgctgcgat ttcttcaatt
     2761 ccttcttctt tcaaagcgtg aatcaagctt tcttgcacca tccccaagcc cttcttcaca
     2821 tcatctgtca aaccttcaac tgcaagtgca cgctcaaggt tgtcaagaga tggtaagatt
     2881 gcttttgcca agtcctggct acgataacgt tgcaagtttt gacgttcttc attggcacgg
     2941 cgttggatat tttgcatttc tgcatgagcg cgaagatatt tgttttcgaa ctcatctgca
     3001 cgttcatttg ccaagtccaa ctcagacttt tcaggagttg tttcttcagc tgtttccaca
     3061 acttcctctt cttgaacttc ttctacttct tcatttttta tatcttgggc catgttcgcc
     3121 tcctttaatg atttcaatct taatgtactt cgtaatgatt actgctgagg tagcggtaaa
     3181 aatctgtcaa cttcatggtc aaaacacggt tgaccacatt gatttgattg attagctgtt
     3241 ggtaatccag attaactgga ccgataatgg ctagaattcc aactccccga taaggaatga
     3301 ggaacttact actaatcacc gctaggtcag ctaaacagga ctcttgaccg tctgcaacac
     3361 gaacattttg catctgatcc tcacgcaacc cctcacgaat ctccaaggcc accttttgcg
     3421 gttggtcaaa gaactgatag gctgctagat tggcaaaatt caagagattg accttgcccg
     3481 ccatcacaat gttttcgttg aacatttcct taaagatgtg ttcaaagaga tcgatgacat
     3541 tatccgttgt tgtaaagtaa cgctggataa tctgcggaat ctccgtccga atcttgtagt
     3601 gaatatctaa aacggtgtga ccgaggaaac gttcctgaat gatgctcttc agtttcagca
     3661 aatcctcctg caagaagttc cttggaatca gaaactgact agtaaccgtt cgcgactcgt
     3721 ctagggtaaa taccgccaag gctgtatgtt gccccaaaac aacgatatca aaggctgtca
     3781 aacgttgcct gctcggctca acatccagtg ccactaccgt acagccactc aggtctgtta
     3841 gcaagttagc agcctcttgc agaatatcct ccaatttgaa gaattcctga tcaaaggctt
     3901 tgacaatctc atatacctca ttttcagcca gtcggtcaaa atccagtgag tgtttcacat
     3961 agtactgaaa accagcaaca cttggcatcc gaccgcttga agtatgagcc ttctcaagaa
     4021 acccttgttt ttctagttcc gccatgtcat tacgaatggt tgcactgcta gagttaatag
     4081 actcttgcaa ggcttttgat ccgacaggtt cgtgcgtttt ggtaaagatg tcaataatca
     4141 gatttaaaat atcctgctga cgctctgtaa ccatctcatc actcctctct gtctgatcaa
     4201 ttagcactca atgcatccaa gtgctaactc atgattctat tatacaccct tagaagagaa
     4261 tgtcaagaca aaaactcaaa aaattagcac tcttttatca agagtgctaa aaatcaatct
     4321 gaagacctag aaccttgcta ttcatctact cggtatttcc tttttagtct gaaataacca
     4381 tgaattagat aagaaatcag aaaagcatat aaaacaaaaa taagaaagaa agacatagaa
     4441 agttcctctc taaacaaact catacaaaca acaagaccac ctaagaaagt agctgtacag
     4501 gaacgaagtc tagatcgcat aacttcccat ttgagcccct tactcatata gacttgatcc
     4561 tctggaagta aatcagaaga cgatttacga agaatcgaac aagaacctgc tcctatcaat
     4621 tcccaacctc tatctctaaa ttcttgcagt tcatgcttat attttttaag aaatctagaa
     4681 tcatagatac ggtagatgac atcgtctggt tgacattggt caaaatagaa caaaccaaaa
     4741 cgactcgttc tatacctcca acctttcaaa tgcatctcat gtaaatattc ttcttccttg
     4801 tccaaatcaa gaatggtgaa aatccgaaat tctactctgc tattcattgt cttaccccaa
     4861 aattagaaaa catgcctggc gttatttatt agataattct ttccactttt gactcaatct
     4921 ccaaaaaata taagaaatct gaatcgcaaa aactatcaat aaaacacaat ctattatgaa
     4981 aatcaaaaat actcctcaac tgaaagaact acctccagtg acaaactttg agaaaaccgg
     5041 tagtagagct aaaaagagaa gcaaaatagg aagcatccgc attgttaaaa tccgtttggc
     5101 ataaaaaatc tttatttaaa ctaaaatatt atagcaaaat ttacgccagt ttttgaacgg
     5161 ctgatgtaga tattttatac tttcaaaatg tttaaatgtg attatttatt tttgaaaaat
     5221 agctcaccag cccgactgaa agtgcttata gaatgataat aagtcgcctg ccgaaaacag
     5281 cggaaaatag cggtgttatg cggagataat ctgacgcgat gcgaaagtat attgcatact
     5341 tattttcaac aatttagcag agtattttta taagtgtgat ataatagaag tataatttgt
     5401 tctgatagtt tattttatgg agaagtagat ttttagaatg cggagggttc aatatggttg
     5461 agtttataaa gtctaagaaa gaaatgagtg aggaggatat taaagcaaat ttcatcactc
     5521 ctgctattgt atccaaagga tggaaaaatg gtgagcatat cgcttacgaa gaatacttca
     5581 ctgatggtcg aattgaggtt agaggagata aggctcgtcg taaagaagga aaaaaatcag
     5641 actattcact gtattaccaa tttggaactc gaattgcaat tgttgaggca aaggataata
     5701 aacacagcgt tcgagcagga ttacaacaag ctattgaata tggagagatt ttagatgttc
     5761 catttgttta ttcttcgaat ggtgatggct ttattgaaca cgaccgtatc acgagagaag
     5821 aacgtgagct ggagttagac gaattcccta ctcgtgaaga attattttct cgtatgacga
     5881 aggaaaaagg attgacgtac gaaattacag aagctatctc aactccatac tatacagacg
     5941 ccttctcaat gaaaacgcca cgctattatc agcaaatagc tatcaaccgt actattgaaa
     6001 cagttgccag aggacaaaaa cgagtaatgt ttgtgatggc aacaggaaca gggaaaacgt
     6061 tcatggcttt tcaaattatt catcgccttc gaaaagctgg tttggctaaa cgagttttat
     6121 tcttagcaga tagaaacatc ttagtagacc aaacgatggc tgaagacttt aagccattcg
     6181 aaaaggtaat gacgaaaatt acaccaaaac ttttgactgc tcctgaaaaa ttaaattctt
     6241 tcgaaattta tctagggctt tatcagcaac taactggtga agatggaact gaaacacatt
     6301 atcaaaaatt tgacaaagac ttctttgatt taatcgtaat tgatgaagcg caccgtggtt
     6361 cagctaagga aaacagtaac tggcgtaagg taattgatta tttcagttct gcgacacaga
     6421 ttgggatgac cgctactcct aaagaaacca agaatgcttc caatacggaa tactttggtg
     6481 agccaatcta tacttatagt ttaaaacagg gaatcgagga tggttttttg gctccatatc
     6541 gtgttatgag ggttaattta gatgtggatg tggatggtta tcgtccagaa actggaaaag
     6601 ttgatgctaa cggacaatta atagaagata ggtactacgg caggaaagat tttgataaaa
     6661 ccattgtcat tgatgataga acgcaaagag ttgccaagtt tgtttctgat tatatgaagc
     6721 aaaacaatgc acgatttgat aaaacaattg ttttttgtgt tgatattgac catgccgagc
     6781 gaatgcgtgc tgcacttgta aaagagaatc tagacttagt ccaagaagac tatcgttatg
     6841 tcatgcaagt aactggtgac aacgctgaag gaaaagctca actggataac tttatggatg
     6901 tcaattctaa ttttcccgct attgtaacaa cgtctaaatt attaacgaca ggagttaatg
     6961 ctaaaacatg tcgtttgatt gttttagact ctaatatcca atccatgact gaatttaaac
     7021 aaattattgg tcgtggcaca cgtctttatc ctcaaaaggg gaaagaattt tttacgatta
     7081 ttgattttcg aaatgttacc aatttgtttg ctgaccctga ttttgatggt gagccagtga
     7141 aggtgctgga aacaggtgcg aaaacagtca gtggttctac gcccggtttc gtagatgagg
     7201 aaggtgaccc agtagaaaaa tatatcgtta cagacaagca ggttaccatt cttaattcta
     7261 ctgttcaagt attggatgaa aacgggaaac tgattaccga aagcctgacc gactacactc
     7321 ggaagaatat cttaggtagc tacgccactt tgaacgattt tatcacagtt tggcatacgg
     7381 cggataaaaa gaagcttatc ttagacgaac tttataaaaa aggagtttat ctagatgcta
     7441 ttcgagagtc ggagggaata tcagaacaag aaatcgatga ttttgattta ctcctaaaac
     7501 ttgcctatgg tcaaaaagaa ttaaccaaaa cggaacgtat caataaactc aaacaaagcg
     7561 gatatttata taaatatagt gaggaagcgc gtgctgtttt ggaaatttta ctgaacaaat
     7621 acatggataa aggtattgga gaactcgaaa gcattgaaac attaaaactt ccagaatttc
     7681 agatatatgg tggaaccttc aaaatcatca atacttattt tggagataaa aaacgatatt
     7741 tacaagcaat taaagaattg gagcaagagc tatttacagt agcttaatga aaggaaagta
     7801 tgtcaattac atcatttgta aaaagaattc aagatatcac tcgaaacgat gctggtgtta
     7861 atggtgatgc tcaacgtatt gagcaaatgt cttggttatt attcttaaaa atttatgata
     7921 gccgtgaaat ggtttgggaa ttagaagaag acgagtatga gtcaattatc ccagaggaat
     7981 taaaatggcg aaattgggct catgctcaaa atggggaacg ggtattgaca ggcgatgaat
     8041 tacttgattt tgtcaataac aagttattca aagagttgaa agagcttgaa ataacttcaa
     8101 ttatgcctat tcgaaaaacg attgttaaat cagcttttga agatgcgaac aactatatga
     8161 aaaatggcgt cttgttacgc caagtcatca atgttattga tgaagttgat ttcaatagcc
     8221 ctgaagatcg tcattcgttt aatgatattt acgaaaaaat tcttaaagat attcaaaatg
     8281 ctgggaactc aggagaattt tatacgccac gtgcagcgac tgattttatt gccgaagttc
     8341 ttaacccaaa acttggagaa tcaatggcag accttgcttg cggaacagga ggcttcttga
     8401 cttcgactct gaaccgttta agtagtcaac gtaaaactag tgaagatacc aaaaaatata
     8461 atacagctgt ttttggtatt gaaaagaaag catttcctca tcttttagca gttacaaatc
     8521 tgtttcttca cgaaattgat gaccctaaaa ttgttcatgg aaatactttg gagaaaaatg
     8581 ttcgtgaata tacggatgat gaaaaatttg acattattat gatgaatcca ccttttggag
     8641 ggtcagaatt agaaacaata aaaaataact ttccagcaga attacggagt tctgaaacag
     8701 ctgatttatt tatggctgtc attatgtatc gtttgaaaga aaatggtcgt gttggagtta
     8761 ttttacctga tggttttcta tttggtgaag gtgtaaaaac tcgcttgaaa caaaaactgg
     8821 tagatgagtt caacttgcat acgattatta ggttgcctca tagtgtcttt gcaccgtata
     8881 caggaatcca tacgaacatt cttttctttg ataaaacaaa gaaaacagaa gaaacttggt
     8941 tttatcgttt agatatgcca gatggttata aaaatttctc gaaaactaag ccgatgaagt
     9001 cagaacactt caatcctgtt cgtgactggt gggaaaatcg tgaagagatt ctggaaggta
     9061 agttctacaa atctaaatca tttacaccta gtgaattggc tgagttgaat tataatttag
     9121 accagtgtga ctttccaaaa gaggaagagg aaatcttaaa tccctttgag ttgattcaga
     9181 attatcaagc ggaaagagca actttaaatc ataagattga taatgtatta gctgatattt
     9241 tgcagttgtt ggaggacaaa taatgacacc agaacaactt aaagcaagta ttctccaaag
     9301 agcgatggaa gggaaattag tgccgcaaaa tcccaatgac gaacctgcaa gtgaattatt
     9361 aaagagaatt aaagctgaaa aagaaaaact tatcagtgaa ggaaaaatca aacgagataa
     9421 aaaggaaact gagatatttc gtggtgatga tg
//
LOCUS       DAVMHG010000074         9194 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMHG010000074 DAVMHG010000000
VERSION     DAVMHG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.074"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(178..981)
                     /locus_tag="VYR08_001377"
     CDS             complement(178..981)
                     /locus_tag="VYR08_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV3466894.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1262..2017)
                     /locus_tag="VYR08_001378"
     CDS             complement(1262..2017)
                     /locus_tag="VYR08_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV3466895.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPCFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2087..4738)
                     /locus_tag="VYR08_001379"
     CDS             complement(2087..4738)
                     /locus_tag="VYR08_001379"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV3466896.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPVFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(4731..5309)
                     /locus_tag="VYR08_001380"
     CDS             complement(4731..5309)
                     /locus_tag="VYR08_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3466897.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5309..5869)
                     /locus_tag="VYR08_001381"
     CDS             complement(5309..5869)
                     /locus_tag="VYR08_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3466898.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5856..6305)
                     /locus_tag="VYR08_001382"
     CDS             complement(5856..6305)
                     /locus_tag="VYR08_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV3466899.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6717..7112)
                     /locus_tag="VYR08_001383"
     CDS             complement(6717..7112)
                     /locus_tag="VYR08_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV3466900.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(7208..7462)
                     /locus_tag="VYR08_001384"
     CDS             complement(7208..7462)
                     /locus_tag="VYR08_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV3466901.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(7472..8806)
                     /locus_tag="VYR08_001385"
     CDS             complement(7472..8806)
                     /locus_tag="VYR08_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV3466902.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8806..9039)
                     /locus_tag="VYR08_001386"
     CDS             complement(8806..9039)
                     /locus_tag="VYR08_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV3466903.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2609 a         2072 c         1757 g         2756 t
ORIGIN      
        1 cggattttta tcgctctgaa catcaaaaaa gaaaggacga aatttgtcct ttctcgaact
       61 tagcttttct tcaacccact acaattgaca aagagccaaa ctttgatgat atacgttttc
      121 ttgtgagaat atttacttca ttttttccta aaattcaatg tttactcagt atttggatta
      181 tgaaaaatcg aggtctaaat ctagatacat tttttctgaa gacaaatcat tttgaccacc
      241 gagcaagaga ttttcaaaaa aagctgttaa aaactcagga cgtcgctgta aaatctttgc
      301 attatctaat accaaggcat cacgaaaata cttggaatgt tgctgaaatg gtatattatc
      361 aatatcaaaa ccaaactcac gaagatactg aatcaaaaag accgttactg tccgagtgtt
      421 tccttcgcga aatggatgaa tctgccagat tcctgaaata aaatgctgga tttgtttaac
      481 cacatccgcc tgagttagtg tcgcatatgc aacttgtttt tcctgattaa aatcataatc
      541 taaggtcatt tgaatcatgg agtaatcaga gtacacaaca ctttcaccat tcaaaacagg
      601 ttcattcttt gtgatattgg tctgacgaaa ttgccccacc ggaatagagg gttcaaatat
      661 atcttgaaac aactccttat gaatagcaag taaggtcgca ggactaaagc taaagcctct
      721 tcgagacaat agttctacaa tacgtagaga aaccaagtct gcctcttccg tacttgcatc
      781 aatagtatgg tgataagccg ttgcatcctc ataaacctgc tcataagtca gttctccccg
      841 ggactgtttc tcagccaaag attccatata cgctgatggc actagattgt caactttctg
      901 cagaccaaaa cctatccgcc ataaatcacg cttcgcttca taagacaagt ttggattgtc
      961 aatgttgtaa gttggttgca tagaaatatc ctttcaattg tttttcttca ttaccagtaa
     1021 atcacgaaat tggttggaaa ctggcttaaa ctataaaaag tcctcacaat aggttctatg
     1081 atatatatat atatatactt catgtaaata ataaaagagt tataatcagc actactgata
     1141 aattcaatac cgatttcgcc atcttaaaac ttagtcaaac gaataccgtt gatttccagt
     1201 tcattaagtc gttgacctgt caagacttct cgttccttca actggaaaag ttaaggggga
     1261 actaatacaa atcctctacg cttctatatt caatgatttc atttttgata atattctttt
     1321 taaactcaaa atgttttaaa ggattatcga ttaacactaa ctttggaata tcatctagat
     1381 ttgtgaccaa ggataaaata aaatccgatt gaaactcctt gactttttca aattcttttg
     1441 cagttaaacg cacacgcccc ttagattttc ggataccttt tacttcggca aggtaagaat
     1501 actcttggac atctacctga aagtcatatc catcaccata aagacgggca tcggttaact
     1561 gtccaccttg gaatttttct tctttatcaa aatgaaggat aaaataattc tcagcctcca
     1621 ttcccgtttc ctgtagacgt ttgaatttag ttcgaactat tggtttttca acgatggtta
     1681 tgcctgtttt cttcccttca ctggcaagta acattttaac tatttcagca taactatgaa
     1741 catcttcatt tccaaacatc atatcaatta aatctttacg aaaacgataa acttcagctt
     1801 tctgccacca accttttcga ttattgtcaa aacaaggatc aaataaatcc attctatttt
     1861 ttacaactcc agttgtttca gcaattccca gagaaaccac atagcgataa aactccgact
     1921 tgctaaagca ttgaaattct tttataaaaa gcttgtcaaa cttggcaaga ccgtagccaa
     1981 ttaaatttaa caattcataa tgagggtgtt tggacatcac tttctccatc aatagatatt
     2041 gatttcatta taccaaaaaa cacggctcat caccgtgttt ctgtgtttat ttcaccaact
     2101 tcttcatctc atcaatacgt gctacggtca catcatactt ggcttggtag tcggcttgtt
     2161 tgtctttttc tttttggaca acttctggtt tagcgttggc gacgaagcgt tcgttagaga
     2221 gtttcttacc aaccatgtcc agttcttttt gccatttagc gagttctttg tcgagacgag
     2281 ctagttcttc ttcaacattg aggagatcgg ccagtggcaa gaagatttcg gctcctgtaa
     2341 taacgcttga catagccagt tcaggtgcag ggatggttga tgcgatttcc aagtgttctg
     2401 gatttgtaaa gcgtttgata tagttgacat tgctgttaaa gaaggcttcc aagtcactat
     2461 cgcttgtctt aacaaggatg gtgataggct tgcttggtgc tacatttact tccgcacgcg
     2521 cattccgaac ggcacggatc aagtctttga gactttccac accagtgtgg gcagcaaggt
     2581 cctcaaagac tgggttgaca gttgggtatg ctgctgtcac gatagagcct tcagcgtact
     2641 gaccaaagat ttcttctgtc acgaacggca ttattgagtg aaggagacga aggattttat
     2701 ccagcgtata gaggagaaca gatcgagtaa ttaccttatc gtcttcattg tcgctgtaaa
     2761 gaacttcctt ggtcaactca acataccagt tggcaaattc ttcccaaatg aagttgtaaa
     2821 ggatatgacc agccacacca aactcgaact tatcaaagtt ttcagtaact tttgcaatgg
     2881 tttcgttgag attgtggaga atccagcggt ccgtcacatt accagcctca cctgttgcaa
     2941 cttttgtgac attgtcatgc gccacatcca gcgtcaaacc tccattgttc atgaggatat
     3001 agcgagaaat gttccaaatc ttgttaataa agttccatga agcatccatt ttctcataag
     3061 agaaacgcac gtcttgtcct ggcgcagaac cgtttgaaag gaaccatcga agggcatcgg
     3121 caccgtattt ctcgataaca tccattgggt caatcccgtt accgagagac ttagacatct
     3181 tgcgtccttg ctcgtcacga ataagaccgt ggataaggac gttttggaat ggttgacggc
     3241 ctgtgaattc caatgactgg aagatcatac gagacaccca gaagaagatg atgtcgtaac
     3301 ctgttaccaa ggttgaagtt gggaaataac gtttaaagtc ttctgagtcg acttcaggcc
     3361 agcccatggt tgaaaatggc cagagggcag aactgaacca agtatccaag acgtcttcgt
     3421 cctgagtcca tccgtcacct tctggagctt cttcgccgac atacatttca ccatcagcat
     3481 tgtaccaggc agggatttgg tgaccccacc aaagctgacg agagataacc cagtcgtgga
     3541 cattttccat ccattgaagg aaggtatcgt tgaaacgagg tgggtagaat tcgaccttgt
     3601 cctctgtgtc ttggttggca atggcgttct tagccaattg gtccatcttg acgaaccatt
     3661 gagtagacaa gcgtggctca actacgacac ctgtacgttc tgagtgacca acactgtgga
     3721 cacgtttttc gattttgaca agggcaccga tttcttccaa cttagcaacg actgccttac
     3781 gagcttcaaa acgatccatg cctgaaaatt caaaggcaag ctcattcata gttccgtcgt
     3841 cgttcatgac gttgacttgt ggcaagttat gacgttggcc aaccaagaag tcatttggat
     3901 cgtgggcagg tgtgattttc acgacaccag taccaaactc aggatctgcg tgctcatctc
     3961 caacgattgg gatgagttta ttagcgattg gaaggatgac gtttttacca atcaagtcct
     4021 tgtagcgcgg gtcttctgga ttaaccgcaa ccgcaacgtc cccaaacatg gtctcaggac
     4081 gagttgtagc aacttcaagg gcgcgtgaac catcttccag catgtaattc atgtggtaga
     4141 aggcaccttc tacatccttg tgaatcacct caatatcaga aagggctgtg cgagctgctg
     4201 ggtcccagtt gatgataaac tcaccacggt agatccagcc tttcttgtaa aggtccacaa
     4261 agaccttacg aacagctttt gacaaacctt cgtcaagagt gaaacgctca cgagagtagt
     4321 ctacagagag ccccatcttg ccccattgtt ccttgatagt agtggcatat tcgtctttcc
     4381 attcccagac tttcgtcaag aaagactcac gaccaaggtc atagcgggaa atgccctcac
     4441 cacgcaagcg ctcctctacc ttagcctgag tggcaatccc tgcgtggtcc atcccaggaa
     4501 gccaaagggt atcaaaacct tgcatgcgtt tttgacggat gataatatct tgcaaagttg
     4561 tatcccaagc gtgaccaagg tgaagtttac ctgtaacgtt tggtggtgga ataacgattg
     4621 aataaggctt agccttttga tcgcctgaag gcttgaaaac atcagcatca agccattttt
     4681 ggtaacgacc agcctcaacc tcggctggat tgtatttaga tgaaagttct ttagacatgt
     4741 gtgtgtcctt tctctatttt gtttatttta ttttgaattt gcttagcagc ttcttctgca
     4801 gacaaattcg tattatttat tttaaagtag tggtgcaact cattcggttg atattgggaa
     4861 tttaattgaa gtgtttcagc ggtctctata atttctcttt cagatacctc aatatgtcgt
     4921 tttaagggtt tgtactttaa tcgattctcc gttcgatttc gacgtatgca ctcttcaaga
     4981 cttgtttcca actcaacaaa aagaatctct tgatgataat catccaacaa actctgaatt
     5041 tgctttaaat atatcagatg atactgattt gaaaaatcaa ttacatctgt taaaattact
     5101 gatcgctgat ttcttgcact cgctccaagg aatgaaaaag tgattccacg aacaaattcc
     5161 cacatttcct ctgtatagtc ctgatagatt tctagtgcaa aatcgatggc ttgatggtta
     5221 taaaataggg tagcatccgt cagtcgagat aattcttgac caatggtcat ttttcctgat
     5281 gctggagcac caatgatgaa aagatgcatc aaatcacctc ccattcactc ttcagcaagg
     5341 catatcttaa atcatcacag cagttgcctt gagcatcttt gcggtctctt atgcgagctt
     5401 cgagggtaaa gccaagcttt tccgcgactc gttgactttg aaggttatat ccaaagcaag
     5461 ttagttcaat cttgtgaaga cccaaatctt taaaggctaa gtcaatcaag gcacgcgcag
     5521 cttctggcac ataacctcgc ccccaatagt ctgagtgtaa ggtatagccg atttccatca
     5581 catcatcttc atggcgatgg ttgaaataga tagagccaac gatcttatcc gttcctttga
     5641 cgacaatccc gtagcctgct gggagatttt ccttttgatt acgctccgga agaatgtgct
     5701 ccagataata aatctcatct tccaagatct tgactggagg aaaacctgct ggataggcga
     5761 cctctggcaa actagcatag tcaaagatat cctcagcatc cgccacagta cggactcgta
     5821 aaacgagacg ttctgtttcg attttatctg gcagctcagt tcttgccatc cttcttcctc
     5881 gcttttttga tgaaactgcc cttcatatct acacgcttgt ccagatagcg ataaacgcgc
     5941 tgatatccat ctcccatgaa ataggttggg gcaaacagtt gatttttaaa atgtcccttt
     6001 tcatccagga gttctggggc aacaagtcgc tcaagaatct tggcaaagat gtggcaaata
     6061 ccgtcttcct caacaatcct atctacccga caatctaaaa caagtggaca ggcgtctaaa
     6121 ataggaatct gagttcgttc agaaatttca taatgcactc ccaaacgttc caatttctcc
     6181 tgatgactga taaaaccagc ctgctccatc gcaagcatag aagtttcatc agaaatattc
     6241 acagtaaatt tttgatactg tttgatctgc tctgcggcat tctctctcgc aacgactcca
     6301 atcacaaccc aatctcctag actataagag gaactacagg tcgtgatgtt atagccaaaa
     6361 ttctaatctt gatatcctaa aataaaaaca ggaaaaccat aatatagttt acttgtgtta
     6421 aaagattgct tcataacaac ctcctttgac taagacacaa aagaaaagcc ctgccatcta
     6481 catgacaggg acgaatgtgt ttatccgcgg taccacccaa tttcgggcca agcccgcatc
     6541 tttagtcttt aaaataaaaa gacagaaata tttcattttt tcagtcatta gcaaccagtt
     6601 ttgacacatt tgtggacttc tcagcgccgc cactttctgt aaaatgtggg attcaaaaca
     6661 cctctaatgg ctctattgta gcaagatttt ttcggaaatg caaggctcaa gaaaaattac
     6721 ttgaacttga tgccattttc tttcaatttc ttgatggtag ctggaccgat tcctttcaag
     6781 tcaaggagtt ctttttcagt ccagtttttg aaatctgaag cagacttgat tccttcatca
     6841 tagaaagtct tggcacgatc aagtggaagt ccacccaagt ttgctgcaaa atcttctaca
     6901 gaattagcta ctttttgagc ttgctctttg gtagcttcta ctgcttgttc aactttttta
     6961 gaaacagcct taccagctgc gcttgctgac tgttctgcac gcttcacgac tttttttgtc
     7021 tcatctacaa ccttagtcac cgtacttgaa aaggcacctg cgcgacggag gccatttcgt
     7081 aattgttttt tacgattgag tttctttgac atgataaaat cctttcaata aaaaatccct
     7141 gctctgacaa ggatttaaca taaacattct atcaagattt tagtgaaaaa tcaagaatct
     7201 ttctcgttta ataatttcgc tcaccaaaca agccttctgg caaatcatgg aatcccttgc
     7261 ctgctttgaa gttttcaaac ttaccaagca agaactgata agcatccaag cgactctcgt
     7321 agcgaatcat ggcatctttg gcacgctcgt cctggtcttc ttcgtaaact ttttgacttt
     7381 ccttgtgcgc catatcgtta atcatgattt gggtattgac ccaagcttca aattccttca
     7441 taaattcttg ttcgtaactc attttttctc cttattctaa tccacgaaaa tagtccgtat
     7501 caatctcacg gtagggctgg atatcctgaa catactccaa cactccttgg aattctccgt
     7561 cttcatcgtg tactgcagca taggtgatgt ggacaaactt acctcgcgac tcagacttga
     7621 accacatttc atacttgtct ttgcttccct cacgaagccc cttcatgata gttttgacct
     7681 tgtccaagta cttaggcgga tggcagagtt cgacattgcg cccgacttga gacggcgtcc
     7741 gtttgaaaat catctcatca gctggcgtat tgtcattgta atactggaaa atatcatctt
     7801 tattgacaaa ggtaatctcc atagggaggt gattgaggat gagattggcc tgctcgactg
     7861 aaagatagcc attaccaaaa gcctgttgac tatggcggtc cagcacagct tccttttcct
     7921 taggggtaaa ggtaatggta aaatggcctt ctggcgtatc tatgacttgt tgaacttgac
     7981 cttctgccgt atctagctgt acaggctcct ctgcaatctt ttcctcaata aagctctgtc
     8041 gttctggcac ccatttctct gacggacgga tgatggcata gccataggca tcgctctcct
     8101 ccgcaatctg aagccagtca tcctgagtaa aagactcaag gagaatcatg aggaggatgg
     8161 actcttcctt gaaaatcata ctttcaaact ctgtcgcaaa agcttcaaaa gcttccttta
     8221 cactgctaat tgacacttct ggtagtgact tggctgtcgt tagagctgtt tgaaagagtt
     8281 ccctaatctg atcatccact ccccacataa ctttgggagg tgaatcgtgt ccatagcgct
     8341 ccatgatagg aaagaagagt tcttccttac gttggtaatg gatgtcaaat tgacccacaa
     8401 gtcccatctg acgcaccaaa cccttacgca tctccgccag catttcctcg tcttccatag
     8461 actcataggt atctaacaat ctacgaatgc gaatcaaggc cgcacggaga gccagatttt
     8521 cttctttgaa gacacgaact gggtgacctg gatgctcagt atctgaaact tcgacacctt
     8581 tgatagcatt tttaaaaaga ttggcatgga catcacagag ttccataaca tcttcaaaag
     8641 tgacgcccga atccgagttc atcagctcgt gctccataag ggaaatctcg atggctgaca
     8701 cgcccgtaaa ggtcgcatca aagcgatctt gaaccgactc aggagaggcg ccattgtgca
     8761 attctaacaa aatatcccgt aggatatgaa tccgttcatc tgtcattagt ctaatccaat
     8821 cacttcgtag ccattcgctt ccagtgtgcg tacaatcttg tccataggag ttcctgctag
     8881 cttagaaccc tgtttaagtg atactttacg accaactgta ttgcgcatta agggattggc
     8941 aaggggttta aaacccaact ccactagaat ttccaagact tctggatgct tgtccaccac
     9001 ttctgcaaca ggaattgaca catcgatgat attgtccatg acgacctcca ttgtatgttc
     9061 taatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggttg ctcaaaacag
     9121 tgttttgagg ttgtagatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg
     9181 gatagaactg acga
//
LOCUS       DAVMHG010000075         9165 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMHG010000075 DAVMHG010000000
VERSION     DAVMHG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.075"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            269..1312
                     /locus_tag="VYR08_001387"
     CDS             269..1312
                     /locus_tag="VYR08_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV3466904.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1479..3566
                     /gene="topA"
                     /locus_tag="VYR08_001388"
     CDS             1479..3566
                     /gene="topA"
                     /locus_tag="VYR08_001388"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV3466905.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3787..4146
                     /locus_tag="VYR08_001389"
     CDS             3787..4146
                     /locus_tag="VYR08_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV3466906.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4239..4871
                     /locus_tag="VYR08_001390"
     CDS             4239..4871
                     /locus_tag="VYR08_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV3466907.1"
                     /translation="MIYEFCVENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            4915..5571
                     /locus_tag="VYR08_001391"
     CDS             4915..5571
                     /locus_tag="VYR08_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV3466908.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMTGSFVASVREACLEILYQIRKTCFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5590..6222
                     /locus_tag="VYR08_001392"
     CDS             5590..6222
                     /locus_tag="VYR08_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV3466909.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGIAVANSLAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6250..6674
                     /locus_tag="VYR08_001393"
                     /pseudo
     CDS             6250..6674
                     /locus_tag="VYR08_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
     gene            6686..7723
                     /gene="leuB"
                     /locus_tag="VYR08_001394"
     CDS             6686..7723
                     /gene="leuB"
                     /locus_tag="VYR08_001394"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV3466910.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEYQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7720..7989
                     /locus_tag="VYR08_001395"
     CDS             7720..7989
                     /locus_tag="VYR08_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV3466911.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            7993..8352
                     /gene="leuD"
                     /locus_tag="VYR08_001396"
     CDS             7993..8352
                     /gene="leuD"
                     /locus_tag="VYR08_001396"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV3466912.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8571..8714
                     /locus_tag="VYR08_001397"
     CDS             8571..8714
                     /locus_tag="VYR08_001397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466913.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            8975..9136
                     /locus_tag="VYR08_001398"
     CDS             8975..9136
                     /locus_tag="VYR08_001398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466914.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
BASE COUNT         2819 a         1618 c         2048 g         2680 t
ORIGIN      
        1 aatggtctaa aatagatgat gatacttttc gaaaatcttt ttacgtcagc tcagctttgc
       61 cttgctgtgt tttgagcaag ctacggttag cttccgagtt tgattttcat ttactagaaa
      121 tgaaactgat gagagatatc agtagacatt tgagtcagga tattatggaa aatgataaaa
      181 agagctcgtg agattggcat atcagactac taaagtattg agtttgttag gattttagcg
      241 actagttagc tgggaaagga agatatttgt gacaaataat aaactgtatt cgttgataga
      301 atttagaaat aagatatatg aagaattaga actttccaga agtgatttag cgattttact
      361 atgtgccatg cttatcgcct ctatcggatt aaatatggat tcgactcccg tgattattgg
      421 agccatgtta atctctcctt tgatgacacc tattctggga gtggggctct ctctagctat
      481 atttgatttt aaattgttaa gaaaatcttt taaaatatta gctattcaaa ttcttgccag
      541 tttaatagct tcaacacttt atttttatct ttctcccatt tcgtatgcta gttcggaaat
      601 tgttgctaga acctctccga ctatttggga tgttctcatt gcttttgtag gagggatagc
      661 aggtattatt ggtgctagga aaaaagagac caataatatt gttcctggtg ttgctattgc
      721 aaccgccttg atgcctcctc tttgtacagt aggttatgct attgcttctg ctaatctaaa
      781 atttatcata ggctcctctt acctattcct catcaattgt agctttattg tcattgcgac
      841 ttatataggt gttaggttga tgatggttaa gaaacattat tttaaagata atgaagaaga
      901 ctctaaaatg cgtaggattt tgcttctagt tgctgttttg ctgatgattc cgagtttcat
      961 ctctgcaacg actttagtga gagaaacgtt gaaaaaagag tcccttaaga aatttatatc
     1021 agagcagttt caggggtata atattttgaa aaaaacctat tctaaaaaga ctcataccct
     1081 aaagctaacc atttcaggaa attatttgac agaagaagaa ctcgatatga tttcaagtaa
     1141 gagaggtgac tatggtttaa gtgatgtttc tgttcaagtt tcacaattgt ctgattcaga
     1201 acaacttagt aaggaagagc tggtggagca tttcttccag tatatcaagg ataaggaagc
     1261 aaaagaaaag gagaaagcca ataaatttga tacagagtct gaggagcaat aatttcttga
     1321 gaatagctgg tttctctcgt gagtcttcta tgtatatcaa aggaagactg aggttttaag
     1381 tataaaactt ttcttctatt atagtagcta agtcttgaca gggttcaaaa aatggtttac
     1441 actttataaa gtttattact ttgaaaaggt gtgatactgt ggctacggca acaaaaaaga
     1501 aaaaatcaac agttaaaaaa aatctagtca tcgtggagtc gcctgctaag gccaagacga
     1561 ttgaaaaata tctaggcaga aactacaagg ttttagccag tgtcgggcat atccgtgatt
     1621 tgaagaaatc cagtatgtcc gtcgatattg aaaataatta tgaaccgcaa tatatcaata
     1681 tccgaggaaa aggccctctt atcaatgact tgaaaaaaga agctaaaaaa gctaataaag
     1741 tttttctcgc gagtgacccg gaccgtgaag gagaagcgat ttcttggcat ttggcccata
     1801 ttctcaactt ggatgaaaat gatgccaacc gtgtggtctt caatgaaatc accaaggatg
     1861 cagtcaaaaa tgcttttaaa gaacctcgta agatcgatat ggacttggtc gatgcccaac
     1921 aagctcgtcg gatcttggat cgcttggtag ggtattcgat ttcgcctatt ttgtggaaga
     1981 aggtcaagaa gggcttgtca gcaggtcgcg ttcagtccat tgcccttaaa ctcatcattg
     2041 accgtgaaaa tgaaatcaat gccttccagc cagaagaata ctggacagtt gatgctgtct
     2101 ttaaaaaggg aaccaaacaa tttcatgctt ccttctatgg agtagatggt aaaaagatga
     2161 aactgaccag caatgacgaa gtcaaggaag tcttgtctcg tctgacgagt aaagactttt
     2221 cagtagatca ggtggataag aaagagcgca agcgcaatgc tcctttaccc tataccactt
     2281 catctatgca gatggatgct gccaataaaa tcaatttccg tactcgaaaa accatgatgg
     2341 ttgcccaaca gctctatgaa ggaattaata tcggttctgg tgttcaaggt ttgattacct
     2401 atatgcgtac cgattcgact cgtatcagtc ctgtagcgca aaatgaggcg gcaagcttca
     2461 ttacggatcg ttttggtagc aagtattcta agcacggtag caaggtcaaa aacgcatcag
     2521 gtgctcagga tgcccatgag gctattcgtc cgtcaagtgt ctttaataca ccagaaagca
     2581 tcgctaagta tctggacaag gatcagctta agctatatac ccttatctgg aatcgttttg
     2641 tagctagcca gatgacagcg gccgtttttg ataccatggc tgttaaattg tctcaaaaag
     2701 gggttcaatt tgctgccaat ggtagtcagg ttaagtttga tggttatctt gccatttata
     2761 atgattctga caagaataaa atgctaccag atatggctgt tggagatgtg gtcaaacagg
     2821 ttaatagcaa accagagcaa catttcaccc aaccgcctgc ccgttattct gaagcaacac
     2881 tgattaaaac cttagaggaa aatggggttg gacgtccatc aacctatgcg ccaaccattg
     2941 aaaccattca gaaacgttat tatgttcgcc tggcagccaa acgttttgaa ccgacagagt
     3001 tgggagaaat tgtcaataag ctcatcgttg aatatttccc agatatcgta aacgtgacct
     3061 tcacagctga aatggaaggt aaactggatg atgtcgaagt tggaaaagag cagtggcgac
     3121 gggtcattga tgctttttac aaaccattct ctaaagaagt tgccaaggct gaagaagaaa
     3181 tggaaaaaat ccagattaag gatgaaccag ctggatttga ctgtgaagtg tgtggcagtc
     3241 caatggtcat taaacttggt cgttttggta aattctacgc ttgtagcaat ttcccagatt
     3301 gccgtcatac ccaagcaatc gtgaaagaga ttggtgttga gtgtccaagc tgtcatcagg
     3361 gacaaattat tgagcgaaaa accaagcgta atcgcctatt ctatggttgc aatcgctatc
     3421 cagaatgtga atttacctct tgggacaagc ctgttggtcg tgactgtcca aaatgtggca
     3481 acttcctcat ggagaaaaaa gtccgtggtg gtggcaagca ggttgtttgt agcaaaggcg
     3541 actacgagga agaaaagatt aaataagagg aggggcccga aagtaaattt caggctcctt
     3601 ttggttagaa cttgacaaaa tacatcattt tatgcgaaac tagaagaaga ttattttata
     3661 ctcaatgaga atcaaagagc aaattagaaa gctagtcgca ggttgctcaa agcactactt
     3721 tgaggttgaa gatggaagct gacctggttt gaagagattt tcgaagagta ttaactagga
     3781 gaagttatgc gtattatcta tctaattatt ggttttttat cgctgacctt ggctattgtt
     3841 ggggttgttt tacccttgtt gcctacaaca cctttccttt tgttgtctat tgcttgtttc
     3901 tccagaagtt ccaagcgatt cgaagattgg ctttatcata ccaagctcta tcaagcatat
     3961 gtagctgatt ttcgtgagac caagtctatt gcgcgtgaac gaaagaaaaa aatcatcgtc
     4021 tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc agtcaaaatc
     4081 ggtctgggtg ctttgaccat ctttattact tattatctct tcaaggtcat tccagacaaa
     4141 gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc ataacaatat
     4201 gatataataa aattgaagta atattcaagg agaatcaaat gatttacgaa ttttgtgttg
     4261 aaaatgtgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt gaactctgtg
     4321 ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca gcggttgaac
     4381 tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtggt ggtgactttg
     4441 tctataatga cctagaaatt gctatcatgc tagaagacat tcgtttgact gctcaggctg
     4501 gaagtcaagg ggttgtattt ggagctttaa ctgctgataa aaagttggat aagcctaatc
     4561 tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt ctttcacatg gcctttgatg
     4621 aactaagtga tgaagatcaa ccggaagcta ttgactggct cagtcaagcc ggtgtcactc
     4681 gtatcctaac tcgtgctggt gtgtctggcg actccttaga aaaacgtttt gttcactatc
     4741 acagaatttt ggagtacgct aaaggtaaaa ttgaaattct accaggtggg gggattgacc
     4801 ttgacaaccg tcaaaccttt atcgaccagg tgggggtaac acaattgcat ggtactaagg
     4861 ttgtttttta aaaaatagaa aggaactgct agctttgggt agcagttttc acttatgttt
     4921 gaaattttta aatcctatca atttaatcaa gaaaaggctc atgattatgg ttttatagaa
     4981 aatagcgaag tctggacata tagttgccag attttgcaag gtgactttgt catgactgtg
     5041 tccatcactg ctgataatgt gaactttcaa gtctttgacc aagagactgg tgacctctat
     5101 cctcacgttc atatggaaag tatgacagga agttttgttg caagtgtccg tgaggcttgt
     5161 ttggagattc tttaccagat acggaagact tgttttgatg tgcaagattt tatctgtcat
     5221 cagactaagc gtatcatgac tcaagttcag gaaaagtatg gaaaccagtt ggagtatctg
     5281 tgggaaaaat cgcctgatac agctgtattg cgccatgaag gcaatcaaaa gtggtatgcc
     5341 gtcttgatga aaatctcttg gaataagctg gaaaagggca gagaaggaca agtggaagca
     5401 gtcaacctca agcatgacca agtagctaat ttgctttcac aaaaggggat ttatccagcc
     5461 ttccatatga gcaagcgcta ctggattagt gtgtcccttg atgatacttt atcagatgaa
     5521 gaagtactgg aattgataga aaaaagttgg aacttaacct ctaaaaaatg aaatatttta
     5581 ataattttca tgaactttca attagctaaa tattctttac tgaagaaatt ttcagaaaat
     5641 ataggattta ccacactaga ggaatatggt gccatcttca aatacctgat tgagaatgtc
     5701 aagacggatc gtcagatcat ctattcgcct cactgtcatg atgacctcgg aatagcagtg
     5761 gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg ttgaagggac tatcaatggt
     5821 attagggagc gagctggaaa tgctgctttg gaagaaattg cagtggctct caatattcgc
     5881 caagattact accaagtaga aaccagtatt gtcctaaatg agaccatcaa tacgtcagaa
     5941 atggtttctc gcttctctgg tattccagtt cctaaaaaca aagccgtcgt tggtggcaat
     6001 accttctccc acgaatctgg tattcaccaa gatggagtcc ttaaaaatcc tctcacttat
     6061 gagatcatca cacctgaatt ggttggtgtt aagattccgc ttggaaaatt atctggtcgc
     6121 catgcttttg ttgagaaact gagagaattg gccctagatt ttacagaaga ggatatcaaa
     6181 ccactctttg ctaagttcaa ggcactggcc gataagaagt aagaaatcac agatgcagat
     6241 actcgagctt tggtagctgg aaccatggtt gaaaatccag aaggcttcca ctttgatgat
     6301 ttacaacttc aaactcatgc agataatgac attgaagcgc tcgttagcct agccaatatg
     6361 gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt ccgttgaagc aatctttaat
     6421 gctatcgata agttctttaa ccaatctgtt cgtttggtgt cctacactat cgatgcggta
     6481 acagatggaa tcgataccca ggatcgggtt ttggtcactg ttgaaaacag agatacagaa
     6541 accatcttta atgcagcagg gcttgatttt gatgtgttga aggcttctgc tattgcctat
     6601 ataaacgcta atacctttgt tcaaaaagag aatgcaggtg cagtgtttct taccacgata
     6661 tgcctagtgt gtaaaggaga aggctatggc aaagaaaata gtagctctag taggagacgg
     6721 aattggccca gaaatcatgg aggctggttt agaagttctg gaggctctag ctgaaaaaac
     6781 aggttttgac tatgagattg acagacgacc gttcggaggt gcagatattg atgcagcagg
     6841 acctccctta cctgatgaaa cccttaaggc aagtagggaa gcagatgcta tcctactagt
     6901 agctatcggt agtcctcagt atgatggagt agcggttcgc cctgaacaag gcctgatggc
     6961 tctccgtaag gaactcaatc tttacgctaa tattcgtcct gtaaaaatct ttgacagtct
     7021 caagtatttg tcaccactca aaccggaacg aatttctggt gtagactttg tcgtggtgcg
     7081 tgaattgaca ggcgagattt actttggaga tcatattctt gaagagcgca aagcgcgtga
     7141 tatcaacgac tatagctatg aggaagtgga gcggattatt cgcaaagcct ttgaaattgc
     7201 aagaaatcgc agaaaaatcg ttactagtat cgataagcaa aatgttctag cgacctcaaa
     7261 actctggcgg aaagtagctg aggaagtcgc acaggatttc ccagatgtaa ccttggaata
     7321 tcagctggta gactcagctg ctatgcttat gattaccaat cctgctaagt ttgatgttat
     7381 tgtaacggag aatctttttg gagatatttt atctgatgaa tcaagcgtct tatctggtac
     7441 acttggggtt atgccatcag ccagtcattc tgaaaatgga ccaagtctct atgaacctat
     7501 tcacggttca gcacctgata ttgcaggtca aggaattgcc aatcctattt ccatgatttt
     7561 atcagtttcc atgatgttga gagatagttt cggacgttat gaggatgcag agcgtatcaa
     7621 acgtgctgtt gagacaagtc tggcggcagg aattttaacg agagatatag gaggtcaggc
     7681 ttcaacaaag gaaatgacgg aagctattat tgcaaggtta tgaagttaga cgaaaaaatt
     7741 actctagtcc ttttgatttg gaatgtcatc attttcttga tttatggtat tgacaaatct
     7801 aaggcaagga gaagagtttg gcgcatccct gagaaaatct tacttatttt agcctttact
     7861 tttggtggtt ttggtgcctg gctagcagga atcatctttc accacaagac tcgaaaatgg
     7921 tactttaaaa tagtttggtt tcttgggatg gtgaccacac tagtagcctt atattttatt
     7981 tggaggtaat ggatggcagg gtcttcgagg gaatacgctg cttgggctct agcggactat
     8041 ggttttaagg tcgtgattgc aggatctttc ggtgacattc attacaataa tgaactcaat
     8101 aatggcatgt tgccaatcgt tcagcctaga gaggttagag agaaactagc ccagctaaaa
     8161 ccaaccgacc aggtaactgt ggacttggaa caacaaaaaa tcatctcacc agttgaagaa
     8221 ttcaccttcg agatagatag cgagtggaaa cataaactcc taaatagttt ggatgatatc
     8281 ggtattacct tgcagtatga agagttgatt gctgcttatg aaaaacaacg accagcctac
     8341 tggcaggatt agaaaaaata gaaaaggaga tatagtaaac tgaaataaga tgtaaacaaa
     8401 tgaattggag cttaacatcc atttccagca attttttaga aactacagtg gactattctg
     8461 gattcaacac attataaaat tatgacaaaa cacattcaca agaaggctac gacattttaa
     8521 aaggtgaggg cggatgtatc gtttgcccta ctaaagttgg ttacattatc atgaccagtg
     8581 acaaggcagg acttgagcgt aagttcgcag ccaaagaacg taagcgtaac aaaccaggtg
     8641 ttgttctctg cggtagcatg gatgaacttt gcgctttagc gcaactcaac ccagaaattg
     8701 aagcattcta ctaaaaacat tgggatgaag atattcttct tggttgtatc cttccttgga
     8761 aaccagaagc ctttgaaaaa ctcaaagcat acggggatgg ccgtgaagaa cttattactg
     8821 atgtacgtgg tactagctgt tttgttatca agtttggaaa agcaggtgaa caattggctg
     8881 ccaagctttg ggaagaaggt aaaatggtct acgcctcatc tgcttcaatg acaaaacgat
     8941 tgaaactcgc tatgagcaag gtgtaatggt gtctatggtc gataaggacg gcaaactcat
     9001 cccagaacaa ggaggagcac gttcaacttc accagctcca gttgtgatcc gtaaagggct
     9061 tgacattgat aaaatcatga tgcacctgtc agatactttt aactcatggg actaccgtca
     9121 ggttgagtat tattaggata gagaagaagt ctagtgttat gagat
//
LOCUS       DAVMHG010000076         9132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMHG010000076 DAVMHG010000000
VERSION     DAVMHG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.076"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..170)
                     /locus_tag="VYR08_001399"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="VYR08_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(216..449)
                     /locus_tag="VYR08_001400"
     CDS             complement(216..449)
                     /locus_tag="VYR08_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV3466915.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VYR08_001401"
     CDS             complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VYR08_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV3466916.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFVVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VYR08_001402"
     CDS             complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VYR08_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV3466917.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2211..3380)
                     /locus_tag="VYR08_001403"
     CDS             complement(2211..3380)
                     /locus_tag="VYR08_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3466918.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3529..4539
                     /locus_tag="VYR08_001404"
     CDS             3529..4539
                     /locus_tag="VYR08_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV3466919.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(4730..5167)
                     /locus_tag="VYR08_001405"
     CDS             complement(4730..5167)
                     /locus_tag="VYR08_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV3466920.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5252..7885)
                     /gene="polA"
                     /locus_tag="VYR08_001406"
     CDS             complement(5252..7885)
                     /gene="polA"
                     /locus_tag="VYR08_001406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV3466921.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8141..8491
                     /locus_tag="VYR08_001407"
     CDS             8141..8491
                     /locus_tag="VYR08_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV3466922.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREDDTH"
     gene            8706..8822
                     /locus_tag="VYR08_001408"
     CDS             8706..8822
                     /locus_tag="VYR08_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV3466923.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            8815..9048
                     /locus_tag="VYR08_001409"
     CDS             8815..9048
                     /locus_tag="VYR08_001409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466924.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLASEVASQQLGMSVSEFEALL"
BASE COUNT         2560 a         2271 c         1718 g         2583 t
ORIGIN      
        1 ttctcaacct tgcttctctc cttagatagc gcatgattac aggctttatc ttcaactgtt
       61 agcggcttga gtttgcttga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
      121 ccttgataac cactgtcagc caagattttt ccagcttgtc cgatatttct catttctaaa
      181 aaccatctac ttccgttgac ttggaactcc gattgctatt ttccttgaat gattttaacc
      241 acatctccta cgttttggag ttggtcaatt tcttcatcgc tgatttcgat actaaattca
      301 tcttccagag tcaagataaa ctccatcaag tcaacagaat ccgcatccaa atcgtctttc
      361 agactcaagg attctgtcac gacaaagtcc tctccctgtc gctcttggat aatggtcaca
      421 atacggtcaa aaatttcttt ttctgtcatc tcttttattc tcctgaaaat tcacgcgcag
      481 tctgggcaac cacgtctgtt tctagcatgg tacggatctg acgaatcgta ctataaacag
      541 ccttggcatc gcttgagcca tgagtcttga caacaggtgc cttaacacca aacaagaccg
      601 ctccaccaac atctgaataa ttgagctgtt ttttcaaacc tctgaggctg tccttgagaa
      661 ggagggcacc tagtttcgct cgaagaccac cacctgtaat agctgtcttg agcaagccca
      721 tgattcccat agctgtccct tcgatggatt tgagcacagc gtttcccgtg aaaccatctg
      781 ccacaacaac atctgcaacg ccattcatca aatcacgcgc ttccacgttt ccgataaagt
      841 tcaaactttc atcagccgcc agtaattcat aagtttcctt acgaagcggg tcgcccttgc
      901 tactctctgt tccgttgttg agcaaaccaa cgcgtggttg cgcaatgcca cggacatttt
      961 tagcatagaa ggaacctaga accgcatatt gatggaggtg ctgggctgta ttttctgcat
     1021 tggcaccaag gtctagcatg tcaaaacctt ttccatcaac ggtaggcaag gtagacatga
     1081 gtccaggacg gtcgatattc ttgatacgac ccacgacgaa gaatcctgct gccaacaagg
     1141 cacctgtatt cccagccgaa aggacagcgt ctgcttcacc atctttgaca gccttggctg
     1201 ccaataccat actggcattt ttcttattcc taatagctct cgtaggttca tcatccgaat
     1261 caatcttctc atccgtatgg ataatgctga cgcgctctgt cgctgtcaga tattgcttga
     1321 ttttagcttc atctccgtaa agttgaacct cgatatctga aaagtcagat agggcttgat
     1381 tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc catggcatct actgcgattt
     1441 ttttcatttc ttttcctctt ttagtaattg tccccagtct gctagggaat caataaattt
     1501 ctttgatttt aggtgaatcc caacgtactc ttcatataat tgatccataa attggcgtag
     1561 ctcttgcttg attccaggct tgagcgaaat ggtctccaaa gtctcaaaat caatagcttg
     1621 aaattgattg agcagatagg ggatattggg attgagatga caacgtctct tatcctcatg
     1681 ataatgctct ggacagaggc aggctccata tttgaaagaa aagtcaaaag cctgaccaac
     1741 ccgatggcag aagacacact cattaaaatt gaggctgatt ccaaatcgag tcaaaatttg
     1801 aatttcaaaa atattggtca aaacctgata atccaagcct gcttccatca actccaaagt
     1861 cttttgcaaa aaagcaaaca agggagcatc ctgctgattg tcctgcaaac tagcatctgc
     1921 aagagctgcc acataggtcg cataggccat gacaaagagg tcactattaa tcttgggaaa
     1981 agtcatgacc tcatgatagt cttcgatgta actgagtccg tcatcattga ttcgcaagag
     2041 aaatcgtgcc agcaccaagg gctgaataac aggcgccagc ttagactgac cagcgtgttt
     2101 gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt ttgacgagct tgtcatcctc
     2161 acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg atagactgaa tcatgcttct
     2221 ctcatgtact cctcaagtcg tttcatggct tctttgatag tctccatgct ggctgcataa
     2281 gataggcgga cgtagccttc cccgtaacgt ccaaaggctg caccagggat aaaggcaacg
     2341 gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt cttgattgta gcccgctgga
     2401 attttagcaa aaatatagaa ggcaccgtct ggtttgataa tctcaaaacc aagagcagtc
     2461 attttttcga tgatatagtc ccgacgctgg atatattcct tcttcatggg ctccgcatca
     2521 tttttaccag ccgtcaaggc ttctaccgca gcatgttgcg ccatggtatt tgcggcagtg
     2581 accaagtact ggtgactctt gattaactgg gctgtgaagg tcgcaggagc gaaaatcagc
     2641 cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac cattgataat aatagcctgg
     2701 tctctcaaca tcgttcctag agacacatgg gcttcgcctg tgtaggtcaa ttctgagtaa
     2761 acctcatcac agacaacaaa aacttcgtac ttgcgtaaaa cagctgccaa ggcctctaac
     2821 tgctctcgac tgtaggtaat tcctgtcgga ttggctggat agttgagaat aaccgccttg
     2881 agcttatcac cctgctccaa aatggccttc tccaacatct caggagtcaa gacaaaacca
     2941 ttttcagtcg tatcaatctc aacaatttct gccccaacta agttaacaat cggttcataa
     3001 cccggataag caggagctgg caaaagtacc ttgtctccct cttccaaaat agccgtcaaa
     3061 gtcgcagata aagcctctgt cgccccaatt gtaaccaaga tttcattttc aggagcatag
     3121 tccagttggt acttttcctt aacaaagtca ctggctgcct gacgtagagt cagcagacca
     3181 ctcatccctg tatagtagga ttggttctga tcaatcgctc gcttggccgc ctccttgaca
     3241 tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac gcaagacccc aggaatctcc
     3301 gaaataacct ggtcaaactg acgaatcaac gaaacttgaa ttttatctaa ctgtttatta
     3361 aagcgcttag ttaagtccat agatacctcc tactttcaaa acgtatctct attatactac
     3421 aaaagccccc cgaccgcaaa aatacattag agaatagctt tccactttct attttacttt
     3481 tcctgtttat aggtctataa tggtaacaga ttctatttgg aggtttttat gtcatttcta
     3541 tcaaaaaatg gagcaggtat cttggcctgc cttctcattt ccatcctatc ttggtactta
     3601 ggaggattct tccctgtgat tggcgcgccc gtttttgcca ttttcatagg catgctccta
     3661 catccctttc tctcgtccta taaacaactg gatgctggtt tgacctttag ttccaagaag
     3721 ttgctccaat atgccgttgt cttgcttggt tttggtctca atatctcgca ggtcttcgca
     3781 gttggccaat cttcactccc tgtcatcctg tccactatct caatagctct gattattgcc
     3841 tacctcttcc agcgtttctt tgcactggat acaaaactgg ctaccttggt tggagtaggt
     3901 tcttctatct gtgggggttc tgccattgca gcgacagcgc ctgttatcca tgctaaggaa
     3961 aaggaagtag cccaagccat ttccgttatc tttttcttca atgtcttggc tgcgctcatc
     4021 tttccaaccc tcggcacctg gcttcatcta tccaatgaag gcttcgccct ctttgcaggg
     4081 actgcggtca acgacacttc ctctgtaacg gctgccgcca gcgcttggga cagtctttac
     4141 caaagcaata ccctcgagtc tgcaaccatt gttaaactca cacgtacttt ggccattatc
     4201 cctatcacgc tctttctatc ctactggcaa agtcgccaac aagaaaacaa gcaaagccta
     4261 caactgaaaa aagtcttccc actttttatc ctttacttta tccttgcctc tctcctcact
     4321 acactactca cctctctagg tgtgtccagt agtttcttta cccctctcaa acaactctct
     4381 aaattcctta ttgtcatggc catgagtgct atcggtctca aaaccaatct ggtcgctatg
     4441 gtcaaatcca gtggaaaatc cattcttctt ggagccatct gctggattgc catcatcctc
     4501 accactcttg gtatgcagac ccttatcggc attttctaac actcttcgaa aatctcttca
     4561 aaccacgtca acgtcgcctt gccgtatata tgttactgac ttcgtcagtt ctatctgcaa
     4621 cctcaaaacg gtgttttgag cagcccgcgg ctagtttcct agtttgctag tgtgctcttt
     4681 gattttcatt gagtataaca caaaaggtag cccatcagct acctttttct tatgcttcct
     4741 caatcaagcg agtatgttct ctcttgatac agcgattcat cacgatatca tcacatccac
     4801 catcacgcaa aatctctttc gcttctaaac tttcaagtcc tagctgtgcc caaaaaatct
     4861 tggcatcagc tttgagaaaa tcacgcgcca catcgggcag aaattcactg cgacgataaa
     4921 cattgacaat atctacagga aaaggaattt cagcgaggct agcataagcc ttttcaccca
     4981 agatttcgcc acctgccgcc ttgggattga ctgggatgat tttatagccc cgagcctgca
     5041 tttcctttgt tactcgattg ctggttgttt cttcacggtc agacaaaccc accacagcaa
     5101 gggttttact cgttgcgaga tactgacgaa tcacaccatc acttggattg ataaattctt
     5161 gactcataga aaacctcctt tttcatcagt atagcacatt ttgaaaaggt ttgcagaatt
     5221 ctactacaaa aaaggaggac tagccccctt tttatttagc ctcgtaccag gttgcccctt
     5281 cattctcatc tgcgataaga ggaacactga gttgaatggc ttcttccatg gtttgtttca
     5341 ccaatttttt tatctctacc aattcagatt taggcacttc aaggacgatt tcatcgtgca
     5401 cttgtaacag catcttagtc tgataaccac ctgcaaccaa ggctttatcc agctgaatca
     5461 tggcaatctt gagaatatct gctgccgaac cctggatagg tgagttgata gcagttcgct
     5521 ccgcaaaacc acgaatattg aagttgcgcg aattgatatc tggcaactca cggcgacgct
     5581 taaagagggt ctctacatag cccttatcac gcgcctcccg caccacttca tccatgtagt
     5641 ttttaatacc tggaaaacgt tcaaagtagg tatcaatgta ggctttggct tccttacgac
     5701 taattcccaa attattagac aagccaaagt ctgaaatccc ataaaccact ccaaagttaa
     5761 ctgccttggc attgcgacgg tcatttgcag tcacattatc aggacgctca atgccaaaga
     5821 cccgcatggc tgtcgaagta tggatatctg ccccctcttg gaaggcctta atcaagtgct
     5881 catccttaga aatatgcgcc aaaacgcgca attcaatctg tgaatagtca gagctgagta
     5941 gcacactatc ctcccactct ggcacaaaag ccttccgaat caagcgccct tgttccaatc
     6001 gggcaggaat attttgcaag tttggatcca cactagacaa acgcccggtc tgggtcaaat
     6061 cctgcacata gcgagtatga atctttccat cagccaaaat ccagtcctgc aagccaatta
     6121 cataagtaga ttgaatctta gcaatttgac ggtaatccag gattttctta acaatcggag
     6181 caataggagc gagacgctcc aaaacatcca ctgctgtcga ataacctgtc ttggttttct
     6241 tagtgtattc tagaggaagt cccaatttct caaagagaag cacgcccaac tgcttaggcg
     6301 agttgacatt aaactcctca ccggccagct cgtaaatctc ttgagtcagt ttttcaatga
     6361 caagctcatt ttcagcctgc atctcaagca aggtctcttt cttgaccata atcccagcaa
     6421 tttccatctt ggcaaggaca aaagccagag gttgctccat atcataaaga agctctaatt
     6481 gcccattttc gctgagtttt tcaagtaaaa taggctctgt ttctaccaaa acagcaagtt
     6541 tacaagctaa gtgttccaag aatttctcac gttcaggaat ggccttttta acacccttac
     6601 cgtagaaagt ttcatcatca accaagtaag tctgaccata aagactagcg atggtcgcaa
     6661 tttcattgtc ctccacagtc gaaaggaggt atttagccaa acggatgtca aaagcaggcg
     6721 cctgcaaatc tacaccaaaa cgttgcaaaa gaactttaac cttcttaaag tcataaactc
     6781 tcagagatgt tttttctaag aaatccttga aaatcgggtc ttgcaacagc tcaagcttgt
     6841 ctgtggcata gagctgatcc ccacaagacc aggcaaatcc aaccaaatta tctgtatggt
     6901 aattctcacc aaaaagctca aagtggaaga tagactcttc actcagcata tcttgactga
     6961 tttggtcaac aatagtaaaa tccaaaccct cagccacatc agctgacgac acatttaaag
     7021 cctgcttgag ctgtttgaag cccatctcat cgtaaaattt cccaagattt tcaacatctg
     7081 gaccactata gaccaagtcc tctaaaccaa tcgcaatcgg tgccttggta tcaatggtcg
     7141 ctagtgtttt agacaaaaag gcctgttcct tgtcattgat gagattttcc ttcatcttag
     7201 aagtcttcat tccatcaata ttttcataaa tcccctcaag cgaaccatgc tccagcaaga
     7261 gcttaatacc cgtcttttca ccgactttgg tcaccccagg gatattatcc gacttatcac
     7321 ccatgagcgc cttgagatcg ataaactgag ctggtgtgag gcccatttct tccatgaggt
     7381 aatctggcgt aaaggcctca aactcagcca cacctttctt ggaaatttca accaccgtat
     7441 gctcatccgt cagctgaatc aaatccttgt ccccactgac aatggtaata tcaaaaccat
     7501 cctgctctgc tagcttatcc agcgtcccaa tgatgtcatc cgcctcatac tgagccagct
     7561 catagtgacg aatccccata tgatccagca actcacgaat gaaaggaaat tgctcacgaa
     7621 actcatcagg agtcttggcc cgaccaccct tatagtccgc atacatctct gtccggaagg
     7681 tcgtctttcc cgcatcaaaa gccaccaaaa tatgactcgg ctcaacccgc tccaacaaat
     7741 gactcaacat caactgaaaa ccataaatcg cattggtatg caaaccagcc gcattcttaa
     7801 aacggtccaa ctgctgatac agcgcaaaaa acgcccgaaa agctacagaa gacccatcaa
     7861 tcaataataa ttttttctta tccatacacc cattataaag gaaagaatca aaaaatacca
     7921 ttgggaagag ctagagcaag tatttttcaa actttttccg aataaataga tagagccaga
     7981 gaatttagta aacctagatt taaaaatgtg ctataatata gtatattgaa tctataatag
     8041 tacaccttga ctgctaaaat atttctataa attaatttga ctttcctgat agagttattc
     8101 acatcttatt tcaactcact atagaaggag gaataggagg attctcagac atccgggcat
     8161 cagcccaact aatgatttga ttgctaagaa aatattcagc aatccagaaa tcacttgtca
     8221 atttattcgc gatatgctgg acttgccagc aaaaaatgtg accattttgg agggaagcga
     8281 tattcacgta ttactctcca tgccttactc ggtgcaggat ttttatacca gtatagacgt
     8341 cttggcggag ttggataacg gtactcaagt aattattgag attcaagtcc atcatcagaa
     8401 ttttttcatc aatcacttgt gggcttacct gtgcagtcag gttaatcaaa atcttgaaaa
     8461 aattcgtcag cgagaagatg atactcacta gatctacaaa cacatcgctc ctgtttacgt
     8521 cattgctatc gtggatagta attatttctc agatgacctg gcttttcata gctttagtat
     8581 gcgcgaagac acaacaggtg aggtattggc gattaccaac aatggacagg aaaaccatct
     8641 ggttaagatg gcattcttgg aattaaaaaa tacagagaaa ccagcaaaga caaggttcgc
     8701 aagccatggt tggagttttt cggcaacaag ccctttaccc agcaaccgca acgagccatt
     8761 acccaagcaa atcaactgct ggactacaag agctggtccg aggaggacag gaaaatgttt
     8821 agtcaactac atatgcgaga agaacaagtc ttgttagcac aggactatgc cttggaaact
     8881 gctagggctg aaggccttga acaaggacta gagcgtggga aagttgaagg aagggcagaa
     8941 aggaaacttt ttgccttcct agacatagta cgccaaggtc ttctggcttc tgaggttgcc
     9001 agccagcaat taggtatgtc agtatctgaa tttgaggcac tgttgtaaaa tggctccata
     9061 atatccatag gggatttacc cactatggat attatggagc ctattttgtg taaaaaaaaa
     9121 gtcccatatg ac
//
LOCUS       DAVMHG010000077         9052 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMHG010000077 DAVMHG010000000
VERSION     DAVMHG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.077"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(370..999)
                     /locus_tag="VYR08_001410"
     CDS             complement(370..999)
                     /locus_tag="VYR08_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3466925.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1044..1283)
                     /locus_tag="VYR08_001411"
     CDS             complement(1044..1283)
                     /locus_tag="VYR08_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466926.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1433..1699)
                     /locus_tag="VYR08_001412"
                     /pseudo
     CDS             complement(1433..1699)
                     /locus_tag="VYR08_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1724..2035)
                     /locus_tag="VYR08_001413"
     CDS             complement(1724..2035)
                     /locus_tag="VYR08_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV3466927.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(1960..2201)
                     /locus_tag="VYR08_001414"
                     /pseudo
     CDS             complement(1960..2201)
                     /locus_tag="VYR08_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2202..2834)
                     /locus_tag="VYR08_001415"
     CDS             complement(2202..2834)
                     /locus_tag="VYR08_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3466928.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(2848..3843)
                     /locus_tag="VYR08_001416"
     CDS             complement(2848..3843)
                     /locus_tag="VYR08_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3466929.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3840..4538)
                     /locus_tag="VYR08_001417"
     CDS             complement(3840..4538)
                     /locus_tag="VYR08_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV3466930.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4615..5625)
                     /locus_tag="VYR08_001418"
     CDS             complement(4615..5625)
                     /locus_tag="VYR08_001418"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV3466931.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5609..6616)
                     /locus_tag="VYR08_001419"
     CDS             complement(5609..6616)
                     /locus_tag="VYR08_001419"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV3466932.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6603..7556)
                     /gene="mvaD"
                     /locus_tag="VYR08_001420"
     CDS             complement(6603..7556)
                     /gene="mvaD"
                     /locus_tag="VYR08_001420"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV3466933.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7538..8416)
                     /gene="mvk"
                     /locus_tag="VYR08_001421"
     CDS             complement(7538..8416)
                     /gene="mvk"
                     /locus_tag="VYR08_001421"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV3466934.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8538..8867)
                     /locus_tag="VYR08_001422"
     CDS             complement(8538..8867)
                     /locus_tag="VYR08_001422"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3466935.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFCKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
BASE COUNT         2662 a         2111 c         1601 g         2678 t
ORIGIN      
        1 tttaaaattt ctaacactct atcactaata ccaattactc tatatgaagc taaacttttc
       61 ggactatcca aaaatacttc gttattggac gattacatta tagtaaaacg aaataagaac
      121 aggacaaatc gatcaggaca gtcaaatcaa tttctaacaa tgttttagaa gtagaggtgt
      181 actattatag tttcaatcta ctatacaaga cgaaattgaa gaaatctttc cattataaaa
      241 cgcatattat caaggttttt aaacacctaa taatatgcgt tttttcctat tttaaagact
      301 tttatccacc tattatacaa agttaagtat ccatcacagt ttacaattag ccttcattac
      361 ctaacccatt caccatcctt caagagagta acctttaaga accgaataaa taaatggcaa
      421 atttcgttca tttaatttaa ctgcactgtt aatttgatta gctccatctc ctaaagtatg
      481 cactcctact acacggtgac tagcatcata aactgtagat ccactagacc cctctaaagt
      541 atcaacttgg taatccaaga acatgccatc atcacttaaa acttgttttt tatctgtata
      601 catttgatga attttaaaat tatatgatgg atagcctgtg atagtcacag ttattcctgt
      661 caaatttttt tgactagtag gaagacccaa agtccctaat tttgcaccaa tgggctcttc
      721 tagaattaat aaagccaagt catattccct tgcatcctta gaatttaaat ttctaaattc
      781 cttcaaataa cgaacttcct ttactttgat ctttccaaat agttcttgac ttggactaac
      841 agccggaaga acataaatat catccgcttc tttcccatag tcatgtctgt agtagttgtg
      901 agccgctgta attaacacat tatctttaat aaaattcgca cttcccctcc cttctacatt
      961 cattccatcg gctactccat aatacttaga taaaaccata gctgaagtcg aatacggata
     1021 ctgtaaagta ttatcaattt taatcaaatc atcattaccg ataatacttc tgattgcttt
     1081 tggtagtatg aaccatacgt tggtgaaatc tcagataatg aagaatcatt agactctgga
     1141 cctttttcta gtgtctcact tacctcatat tcttcacctg tactagaaat aacactcaaa
     1201 gcagatactg tcgataactg gctagccaat aaagtactcg caataattga aatacccaat
     1261 tttttataaa cagttttctt cattattgtg tcctcctaat gtaattatag cgtactattc
     1321 taaatttctt aatctactat agaatcaaga aatctaccac cttctttaaa taccctccat
     1381 tatcacataa acaggtaaac ttttcaatta atgactgcgc ttttcaatca cgctagaggt
     1441 acttacttgc ttctttgata ctaagttcag ccattctttc cttgtttttc tcaataaagc
     1501 atgttaccca agtgggattc gttttggagt agtctcgcag agtccagcca atggctttat
     1561 tgataaaaaa ttctgtttgg ttcaagttat gaaggagaat cttttccatt aattgagtat
     1621 tggtcttctc ttttcttaac aactggtggt caatagcgac acgtctcagc cagatattat
     1681 ctgataggct ccattttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
     1741 gattgctcaa aacactgttt tgaggttgca gatagagctg acgtggtttg aagagatttt
     1801 cgaagaatat taagattatt tcttctagtt cagggtgttt atacaccaaa ctccctacta
     1861 ctcgatctag gatatctacc gtgtcccacc aggattttgt cacgactaac tgctctagct
     1921 taggcaaatc ggtttccttt agataagact gcattgcttt caaatagtta gcagccacat
     1981 attggtattt tctaggctcc ttttcccagc aagtgtctgc aaaatcccaa tcgataatct
     2041 ttgttttttt cgcttctgga aaatattttt atagagttta tttctttcag gcaccgcaat
     2101 acctagaaaa gaaaattgat ggcgcatata ggcttccatg ggccttgctt tttagagtct
     2161 tttgctgctt ctagctcctc aagtaaatct gctaaactca tctaaaactc ctcttgcccc
     2221 accaaatggt gctgaaaggc atagacagcc gcctgagtac gatcgctgac ttcaagtttg
     2281 gcaagaatgt tggacacgtg ggtcttgacc gtcttgagag agataaagag gtcatctgcg
     2341 atgcgctgat tttcgtagcc cttggcgatg agttggagaa catctcgctc acgcgcagtc
     2401 aattcttcat gaagttccat atgattgcgg tggtattcaa ccttcttgct aacctcttgc
     2461 tcaatggcca gctcgccagc agctacctta ctgacggcat gaagcaattc atctgcacta
     2521 gaagtcttga gcatatagcc tttggcacca gcatctaaga ctggcatgat tttttcattg
     2581 tccaaataag aggtcacaat caaaatcttg gcttcaggcc attctttaag gattgctaag
     2641 gtcgcgtcaa tcccattcat ctcaggcatg acaatatcca tgacaatgac atctggacgc
     2701 agttccaagg ccaagtcaat cccttgagac ccgttggacg cctcacccac aacttctaca
     2761 tcgtcttgga ggtcaaagta gcttttcaag cccaaacgga ccatttcatg gtcatctact
     2821 agtaaaattt tcatctttac tcctttatca ttccttatct aacaggggaa tacggatatc
     2881 aactgccaac ccttgcttgg gagctgttaa taactgaacc gtccctgcca tatcttcaac
     2941 ccgctccttg atatttcgca gtccataact caagtcgtct aagctcccta actggaaacc
     3001 aatcccattg tccaccacct tcagttgcaa ttcaacatct gtctgataga ggtagacatc
     3061 taggcaagat gcctgggcat ggcggagcgt attgctaatc aactcttgca ggatacggaa
     3121 gatatgctcc tcgattttct taggcaattt cgtcatattc tgcttgagac taaccctaag
     3181 atcactcttg tcctcaagct cttttaagag aatttgaatc ccttctatca agctcttctg
     3241 ctccagttca actggtcgca aatgcaagag caaaacccgc aaatccttct gggcagtttc
     3301 taaaatagct gtgacactct gcaactggat ctgcatcttt tctctatcca atttcaaagc
     3361 ctgctgactg atacccgata aaatcatgtg ggccgcaaac aactcctgac tgactgtatc
     3421 gtgcaaatcc cgagcaattc gcttccgttc cttctcgatg atttcctctt cctgagcaag
     3481 gctatgattt tcagcttttt gaagagcttc tgtcaaaagg ttaagtttac ctgataagga
     3541 cttgaaactg gcatccaaat ctggatctgc aacctgaacc acttcttgcc ctgccaataa
     3601 acgcttgaga ttagcctgca tttttcttag agaaagctct tcgatccctc gccaaaacag
     3661 ggctaagaga caggttatgg acatgctgaa aaccaacaat aaaaagacaa atttttctgt
     3721 tttttcgaca tcgtgcaaaa agatagacca gtcaaaatca agtatttcca gcaagctgtg
     3781 ggagaaaaaa aagacaaaca ggaaggaggt gagagcaata atgacatagg cttgtttttt
     3841 catcctctaa ccacctccac atcaccaatc atagtggtca agaaaatctt gacactcttg
     3901 ttactcttga gatagtcttt tgtttcttga tgatagtgtt cattgcggag ggctcgcttg
     3961 ggctggttga aaaaagtcaa atccccatag agacagttaa cgctgagact gacttccaca
     4021 tctacaggta cgatgatttt ggtcgttcct accatctttc tgaggataat gacattgtca
     4081 tgattggtta agatgaccct ctccagatga atagtgtcct tgcccatgaa gcgaaagaga
     4141 ttgatatcat cgaattggca agtctggtag cttgaaaaat gatgaagatt tccaaaccaa
     4201 cgatttttct ccttcttaac cgtcacgacc tcttcaaaaa ccaaattggt ctgctctttt
     4261 tcctggttca tcatcgggta aagaagaaag aggctataga taaccgcaac aaaaatagct
     4321 agaatcacaa aaggattgag cataacgatg aaaaagaaga gaatggttgc cgctactaaa
     4381 agaagattat ttccctcttt accagtgtag tagcgaatca aaagcaaaaa gaggaatagt
     4441 atcagcagaa aacgcgaaaa atgctctgat accatcaaaa tcagagctcc tgtcagaaga
     4501 caggcttcga taaataaaaa gattttaaat tttctcatag gttcatcctc tcccttctat
     4561 tttatcacaa ttcaaaaaag tcacctcagt ctgaggatgg aaaaaaggcg ctggttacgc
     4621 ctttttcatc tgatcctttg cttcttttaa ttttccataa agaagatagt ctactttttg
     4681 tagatctgct atggtggcac agttaaggga acacataatc aagcgtaggt ctgctttcca
     4741 gccttggaca atgccaatca cttcttcaac tgtgtaggtt tcaaccaatt ccagaacggt
     4801 tcgtgacaat cccacagact tagcaccaaa aaccaagcac ttaatcatat ccagcggatt
     4861 ccgaacccct ccactaacca agagttcgac cttatctttc cattcttggg cattgagaag
     4921 ggcctgcatg gtagactgac cccattgatt gaggtaatca cgctggccac tacgacggtt
     4981 ttcgatatag gcaaagctgg tgccaccacg acccgatagg tccactgtac gaacaccgaa
     5041 ttcataggct ctttcgattg tcttggcatc cattccaaag cccacttcct tgaggacaat
     5101 aggaacggga atttgctcgc tataatctgc tagatgcgat tgccagcttc taaacttcct
     5161 ttctccctcg ggcatgagta attcctgcat gacattgaca tgcacttgca atagaacagg
     5221 attcatctct tctacagtct gaagtcctaa ctcgacaggc ttgtccaatc caatattggt
     5281 tccaaggagg agattgggat gactagactt gacagaaaaa gaatcatccg ttggattttt
     5341 gagggctgcg ctataagaac ccgttacaaa taaaatacca caggattccg ccacctgagc
     5401 cagcttttga ttgatttctc ttcccttatt acttccacca gtcatggcat tgatataaaa
     5461 aggaaagtcc cactttcgac cagcaaactc tgtcgaaaga tcgatttcat ccagattgta
     5521 aagaggcaag gaagaatgaa tcagctccac ctcatcaaag ctattatagg aacttttctg
     5581 ctcaagggca tagaggatat gctcgtcctt acgatttgtc gtcatgtcct atcctttctt
     5641 gatataagag ctcaatcccc agatcggccc aacgattttt taaggttttg gttgattgcg
     5701 catcaaaact caaggcgatg ccacagtcac caccaccagc accactactc ttggcaacgg
     5761 cctgcaaatc ttgactggct tctttcaact gtctaagcaa aggcgtgtaa atatctgtac
     5821 tcaagccttc taaaagcttg ctggctactt ctacttgctc gataactttt tcggctttcc
     5881 cctgctccaa ggcttcgacc aaagaaacca ccgtttcttt tgaggaactt aaaaaatttt
     5941 gattgatatt ttgcttgatt tgctggacca tgtgactcga tacagccact tccttggtcc
     6001 atcccactaa gaaatcacat tctaaagttg gtttcacttg tgagataaaa aatccccaat
     6061 cacgctccag aactgtcgcc aagttttctt cttctaacca agcagccacc ttctggcgat
     6121 caaatgactg gtagagaacc aaatcctctg ccacaataca ggcaaggtcg cccatggaac
     6181 cattgtctcc tcgcttgagc aagacagcgc tagtcagctt gaacaagaga ttctgatcaa
     6241 ccgaaagatt atagagagcc agtaaagcct tgacaaccaa gacaacgacg ctgccactag
     6301 aacctagacc aaactttttc ccttctcgtt ccattttgcc acagattttt agggaaaaag
     6361 gtcttaaatt ctgaccgcga acagcgagga agtctcccat caaagcaatc gtttcttgaa
     6421 tcaagctgta gtcaggatta ggccttaagt ccactgcgaa atcaaacata tctgaataga
     6481 tacggtagct gtcagaaaaa gcaatttcag ccctcatata gatgggaata tcctttatca
     6541 aagctaactg ccctggctct aaaatagcat attcacctgc ccaatagagt tttccgcaag
     6601 ttttaacagc aatcatcttg actcaaatcc tttgtttttg acacaatcaa gcgataacgc
     6661 tgaccgaaaa tttctgataa atgctccaag tctttctcct gacagaagac cttaacattg
     6721 ggaccagcat ccatggtaaa gtagcaggcc tctcctttct cacgaagctg gcgaacaaag
     6781 tccatagcct cataagaggc atccgtcaga taagaaaagg ctggactagc agtctttgtc
     6841 gtagcatgca tagccagggc atttttctcc gttaattctc caatcttggc aaaatcattt
     6901 tccttgagat aaatcagcat atcctgatag tccttctcag actgacgaac ccagtcgtcg
     6961 aaagtcgtcg aggtttccac acaaagtttc atcccgtcac ggctagagat tggttttttc
     7021 ttgtcctcta gcaccaacat aatcatagct agtttcaagt ctgtctctac agggtaaatt
     7081 tctccactat ccttatccca ggctcctagt ggtccataaa aactccgaga agaagagcct
     7141 gaggcaaact tggcttcctg cgctaactga cttctatcca atccaagctt gaaataagca
     7201 ttacaagcct tgaccagggc ggacaaacca ctagaacttg aggacaggcc cgctgccgta
     7261 ggcatattgt tttgagtatc gatacggaca aagccctcac cagctggacg ataacggtca
     7321 ataatcttac tcatcttggc atgctcgacc tcattttgta gctgaccatt gatgtaaaat
     7381 tcgtcagctg ttacattggc tggtaaaggc gacaaggtcg tctctgtata catattttcc
     7441 aaagttagag aaatactgct agtagcaggc accatctctt tttctttttt ctttccccaa
     7501 tatttgataa tagcaatatt tgcgtaggaa cgtactgtta caggctctct atccatgtct
     7561 gaacagctcc tttctcttct aatctttctg ctagttcttg tgcgtgtgtc aaattggtta
     7621 ccaaggctat gatacaacct cctagcccac caccgctcat cttggcaccc agagcaccat
     7681 ggctaagagt cgtttcaacc aaaaagtctg cctcagggct actgactcca atttctttta
     7741 aatgtaaatg cgcttgactg aggatttgtc ccagtccttc agcatctttt tgtgaaatcg
     7801 caacttctgc ttgctgggtt aattctccca aggcatgcaa aaacggtagg gcatccttgc
     7861 ccttattttg aaccacttgg atggcttcac gagtatgacc ataaacaccc gtatcggcaa
     7921 tcaccaaata ggcggataaa tccatctcaa gttctgtaaa tcctacgttc ttgataaagc
     7981 gaataggttg gtcactgaga caggtcttag catccaaacc actaggattc atatgggcaa
     8041 tcatttcagc tcgattgacc aagatttcta gtacatcatg aggcagatca gcctgatagt
     8101 agtcaaatac cgcacgaatg gccgctatgc tgatagccgc tgacgaaccc atcccccgtt
     8161 tctcagggat agccgagtca atctcacaac gaatgcaggc ttctgtgata tccaaatact
     8221 ccagtgaggc ataaaccgcc atggacaagg tatcctcctc ataaaggcgc caaggactct
     8281 ctgcaggaac taccttacag gtcacctcca cctccaaaag aggcagggaa atggcaggat
     8341 aaccgtaaac gaccgcatgt tcccctatta aaattatctt actatgtgcc tgaccgacac
     8401 caactttttt tgtcattttt tccttttact agacgaaaaa acgtcttatt tttcatacaa
     8461 gtattaattc tttcctatct attttattat attttcacaa aaaaagcgat tgtttccatt
     8521 cacaatcgct tctttcatta ccgaacccat tcaccattat aattgacaga atagccatct
     8581 acagtcgtat tcactgccaa ggcacctgag ctataagcgt agtaccatct gccattgacc
     8641 tggaaccaac ctgtcgtcat agaacgatga aagaaactcc ataccattaa gtaaagagga
     8701 aagtcatgag ggagcatgcg ccattgacaa cctgttttag tgacgtacaa agtttcatta
     8761 acaagtactc gtttcggcca tttataggtg cggtgtttgc agaaataggg ttcaatcttc
     8821 gcccattctt gatcgtttaa atcagtatca tatgctttgc gtgtcataac tctagtttaa
     8881 catttttttg tgaatacagg ttctaaataa tcgaccacga aaatttctta agtggaaaac
     8941 gccttatgaa gtatgctacg ggaaacttat gcacttaatt tgacaactca atatgtaaaa
     9001 atatatacta tattagattg aaactagaat agtacacctc tacttctaaa ac
//
LOCUS       DAVMHG010000078         8937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMHG010000078 DAVMHG010000000
VERSION     DAVMHG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.078"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            84..1355
                     /locus_tag="VYR08_001423"
     CDS             84..1355
                     /locus_tag="VYR08_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3466936.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1466..2758
                     /locus_tag="VYR08_001424"
     CDS             1466..2758
                     /locus_tag="VYR08_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3466937.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2760..3602
                     /locus_tag="VYR08_001425"
     CDS             2760..3602
                     /locus_tag="VYR08_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3466938.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3855..4655
                     /locus_tag="VYR08_001426"
     CDS             3855..4655
                     /locus_tag="VYR08_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV3466939.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4665..5651
                     /locus_tag="VYR08_001427"
     CDS             4665..5651
                     /locus_tag="VYR08_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3466940.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5787..5867
                     /locus_tag="VYR08_001428"
                     /pseudo
     CDS             5787..5867
                     /locus_tag="VYR08_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6077..7018)
                     /locus_tag="VYR08_001429"
     CDS             complement(6077..7018)
                     /locus_tag="VYR08_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3466941.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(6996..8759)
                     /gene="aspS"
                     /locus_tag="VYR08_001430"
     CDS             complement(6996..8759)
                     /gene="aspS"
                     /locus_tag="VYR08_001430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV3466942.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT         2601 a         2029 c         1686 g         2621 t
ORIGIN      
        1 atttgtgcta tactaagctc ataaaggaaa acgtttgcgt ttccttatca ataaaacttg
       61 ctattcttta ggaggaatac actatgtcat ctaaatttat gaagagcact gcggtgcttg
      121 gaactgttac acttgctagc ttgcttttgg tagcttgcgg aagcaaaact gctgataagc
      181 ctgctgattc tggttcatct gaagtcaaag aactcactgt atatgtagac gagggatata
      241 agagctatat tgaagaggtt gctaaagctt atgaaaaaga agctggagta aaagtcactc
      301 ttaaaactgg tgatgctcta ggaggtcttg ataaactttc tcttgacaac caatctggta
      361 atgtccctga tgttatgatg gctccatacg accgtgtagg tagccttggt tctgacggac
      421 aactttcaga agtgaaattg agcgatggtg ctaaaacaga cgacacaact aaatctcttg
      481 taacagctgc taatggtaaa gtttacggtg ctcctgccgt tatcgagtca cttgttatgt
      541 actacaacaa agacttggtg aaagatgctc caaaaacatt tgctgacttg gaaaaccttg
      601 ctaaagatag caaatatgca ttcgctggtg aagatggtaa aactactgcc ttcctagctg
      661 actggacaaa cttctactat acatatggac ttcttgccgg taacggtgct tacgtctttg
      721 gccaaaacgg taaagacgct aaagacatcg gtcttgcaaa cgacggttct atcgtaggta
      781 tcaactacgc taaatcttgg tacgaaaaat ggcctaaagg tatgcaagat acagaaggtg
      841 ctggaaactt aatccaaact caattccaag aaggtaaaac agctgctatc atcgacggac
      901 cttggaaagc tcaagccttt aaagatgcta aagtaaacta cggagttgca actatcccaa
      961 ctcttccaaa tggaaaagaa tatgctgcat tcggtggtgg taaagcttgg gtcattcctc
     1021 aagccgttaa gaaccttgaa gcttctcaaa aatttgtaga cttccttgtt gcaactgaac
     1081 aacaaaaagt attatatgat aagactaacg aaatcccagc taatactgag gctcgttcat
     1141 acgctgaagg taaaaacgat gagttgacaa cagctgttat caaacagttc aagaacactc
     1201 aaccactgcc aaacatctct caaatgtctg cagtttggga tccagcgaaa aatatgctct
     1261 ttgatgctgt aagtggtcaa aaagatgcta aaacagctgc taacgatgct gtaacattga
     1321 tcaaagaaac aatcaaacaa aaatttggtg aataaaaaat ttgttcaagg ggggtggaaa
     1381 tcaaatcccc ctttgaattt atcaatagag acacaaataa tttagctttc ttataaaaaa
     1441 gtagtatcct atgaaaggag ttaatatgga aaagcaacaa cctagtaaag cagccctgct
     1501 gtctatcatt cctgggttag gacagattta caataaacaa aaagccaaag gttttatctt
     1561 ccttggtgta accatcgtat ttgtccttta cttcctagca cttgcaaccc ctgaattgag
     1621 caacctcatc actcttggtg acaaaccagg tcgtgataat tccctcttta tgctgattcg
     1681 tggtgccttc catctaatct ttgtaatcgt ttatgtactc ttttatttct caaatatcaa
     1741 agatgcacat acgattgcaa aacgcattaa caatggaatt ccagttccac gcacactcaa
     1801 agacatgatc aaagggattt atgaaaatgg cttcccttac ctcttgatca ttccatctta
     1861 tgttgccatg accttcgcga ttatcttccc agttatcgta accttgatga tcgcctttac
     1921 caactacgac ttccaacact tgccaccaaa caagttgttg gactgggttg gtttgaccaa
     1981 ctttacaaac atttggagct tgagtacctt ccgttctgcc tttggttctg ttctttcttg
     2041 gactatcatt tgggctttgg cagcttctac tttacaaatc gtaattggta tcttcacagc
     2101 tatcattgcc aaccaaccat ttatcaaagg aaaacgtatc tttggtgtta ttttccttct
     2161 tccttgggct gtcccagcct tcatcactat cttgacattc tcaaacatgt ttaacgatag
     2221 tgtcggtgct atcaacactc aagtattgcc aatcttggct aaattccttc ctttccttga
     2281 tggagctctt attccttgga aaacagaccc aacttggact aagattgcct tgattatgat
     2341 gcaaggttgg ctcggattcc catacatcta cgttttgact ttgggtatct tgcaatctat
     2401 tcctaacgac ctttacgaag cagcttatat tgacggtgcc aacgcttggc aaaaattccg
     2461 caacatcact ttcccaatga ttttggctgt tgcggcacct actttgatta gccaatacac
     2521 cttcaacttt aacaacttct ctatcatgta cctcttcaat ggtggaggac ctggtagtgt
     2581 cggaggtgga gctggttcaa ccgatatctt gatctcatgg atctaccgtt tgacaacagg
     2641 tacatctcct caatactcaa tggcggcagc tgttaccttg attatctcta tcattgtcat
     2701 ctcaatctct atgatcgcat tcaagaaact acacgcattt gatatggagg acgtctaaga
     2761 tgaataactc aattaaactc aaacgtagac tgactcaaag ccttacttac ctttacctga
     2821 ttggtctatc aattgtaatt atctatccac tgttgattac cattatgtca gcctttaaag
     2881 caggtaacgt ctcagccttt aaactagata ctaatatcga cctcaatttt gataacttta
     2941 aaggcctctt cactgaaacc ttgtacggta cttggtacct caacactttg attatcgcct
     3001 taattaccat ggctgttcaa acaagtatca tcgtacttgc tggttatgcc tacagccgtt
     3061 acaacttctt ggctcgtaaa caaagtttgg tcttcttctt gatcatccaa atggtgccaa
     3121 ctatggccgc tttgacagcc ttcttcgtta tggcgcttat gttgaacgcc cttaaccaca
     3181 actggttcct catcttcctc tacgttggtg gtggtatccc gatgaatgct tggctcatga
     3241 aaggctactt cgatacagtg ccaatgtctt tagacgaatc tgcaaaacta gacggtgcag
     3301 gacacttccg ccgcttctgg caaattgttc taccacttgt tcgcccaatg gttgccgtac
     3361 aagctctctg ggccttcatg ggacctttcg gggactacat cctctctagt ttcttgcttc
     3421 gtgagaaaga atactttact gttgccgtag gtctccaaac cttcgttaac aatgcgaaaa
     3481 acttgaagat tgcctacttc tcagcaggtg ctatcctcat cgcccttcca atctgtattc
     3541 tcttcttttt cctacaaaag aactttgttt caggacttac aagtggtggc gacaagggat
     3601 aatttatccc cgccaccctt tttcatttta tactcttcga aaatctcttc aaaccacgtc
     3661 agctttatct ccaacctcaa agttgtgctt tgagcaacct gtggctagtt tgcactttga
     3721 ttttcattga ttattagcaa ttgtcactgt aaataatatc cttgtagcaa gcaatttttc
     3781 tcctagactt gaaataaagc gcatttctct atataataat actcatatag aaaacacctt
     3841 ttagaaagat acctatgctt ccatatccat tttcctattt ttcaagtatt tgggggtttc
     3901 gtaagcccct gtccaaacgt ttcgagctca actggtttca acttctcttt accagtatct
     3961 tccttatcag cttgtctatg gtacccattg ctatccaaaa cagctcccag gagacctatc
     4021 cgctagaaac ttttatcgat aatgtctatg aacctctgac agataaggtt gtccaggatc
     4081 tctctgaaca tgctacaatt gtcgatggca cattaactta tactggaaca gctagtcaag
     4141 ccccttctgt tgtgattggt ccaagtcaaa tcaaggaatt acctaaggac ttgcaactgc
     4201 atttcgatac aaatgagcta gtcatcagca aggaaagcaa ggaactgacc cgcatctctt
     4261 accgagccat tcagactgag agtttcaaaa gcaaagacag cttgacccaa gcaatttcta
     4321 aagactggta ccaacaaaat cgtgtctata tcagcctctt cctagttctc ggtgcgagct
     4381 tcctctttgg tttgaatttc tttatcgtct ctctaggagc tagtcttctc ctttatatca
     4441 ctaagaaatc acgcctcttt tcatttagaa cctttaaaga gtgctatcac tttatcttga
     4501 actgtttagg attgccgact ctgattacac ttattttggg attatttggc caaaatatga
     4561 caaccctgat tactgtacaa aatattcttt ttgttctgta tctggtcact atcttttata
     4621 aaacacattt ccgtgatcca aattaccata aataggagat ttttatgccc gttacgatta
     4681 aagacgtggc caaggctgct ggtgtttcgc cttcaaccgt aacccgtgtt attcaaaata
     4741 aatcaaccat tagcgacgaa acaaaaaaac gcgttcgcaa agctatgaag gaactcaact
     4801 accacccaaa cctcaacgct cgtagcttgg taagcagcta tactcaggtt atcggattag
     4861 ttcttcctga tgactcagac gccttctacc agaatccttt ctttccatcg gttctacgtg
     4921 gcatctctca agtcgcatct gaaaaccact atgccattca gatagcaaca gggaaagatg
     4981 agaaggagcg tctcaacgct atttcacaaa tggtctacgg caagcgtgta gatgggctaa
     5041 tttttctcta tgcccaagaa gaagaccctc tcgtaaaact cgtcgcagaa gaacagttcc
     5101 ccttccttat cttaggtaaa tctctatctc ctttcatccc acttgtcgac aacgacaatg
     5161 ttcaagctgg ttttgatgcg actgaatatt tcatcaaaaa aggctgcaaa cgcattgcct
     5221 ttatcggagg aagtaaaaag ctcttcgtga ccaaagaccg tttaacaggc tatgaacagg
     5281 cgcttaaaca ttacaaactt accactgaca acaatcgcat ctactttgcc gacgagtttc
     5341 tggaagaaaa gggctataaa tttagcaagc gattattcaa gcacgatcca caaattgatg
     5401 ctatcatcac aaccgatagc ctcctagctg aaggtgtttg taactatatt gccaaacacc
     5461 agctggatgt ccctgttctc agctttgact cggttaatcc caagctcaac ttggcagcct
     5521 atgtcgatat caatagttta gagcttggtc gtgtttccct tgaaactatt ctccagatta
     5581 ttaatgataa taaaaacaat aaacaaattt gttaccgtca attgatcgcc cacaaaatta
     5641 tcgaaaaata agagactggg caaaaagtcg ttaaaagcaa aaacgcatac tatcaggtat
     5701 tgaaaaaact tgatactatg cgttttattg tgggaagatt tacttccttt tctactgaaa
     5761 ttgagtcttt tcccaagatc tttttatact caatgaaaat caaagtgcaa actaggaagc
     5821 tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tgaaactgac gaagtcagct
     5881 caaaacatgg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa catggttttg
     5941 aggttgtaga tgaaactgac gaagtcagct caaaacatgg ttttgaggtt gcagatagaa
     6001 ctgacgaagt cagtaacata tatacggcaa ggtgaagctg acgtggtttg aagagatttt
     6061 cgaagagtat taatcactaa ttatctatct caacaaatct tcctagaata tgaacatttt
     6121 ccgagacaga gacaaaggag cttggatcca cttgtgtcat aatctgttta aattcattaa
     6181 actctgcacg tgtaatgaca gtgattaaaa ctgcctttct ctcgtgatta taggttcctt
     6241 ctgcatcgtg gatcatggtt gctccgcggt gcaatttttt atggattttt tcaattacct
     6301 tctctggatg atttgtcaca atcatggcct gcatacgctt ttgcttagta aagactgcgt
     6361 ctgtcacacg gctagagaca aagatggtaa tcatagaata aagagcgtat ttccaaccaa
     6421 aggtcaaacc tgctatcagc atgatagttc catttaccaa gaaagaaata ctaccgacat
     6481 tcttacccgt tttcttgcga atagtcaggc tgacgatatc cgtcccacca ctggagatat
     6541 tgtttcgaag agcaaaacca atccccaaac ccataacaac acccccaaaa agggaattga
     6601 taatgggatc ctccgtcaag gttgccacag ggacaaactg gataaagaag gaactcatag
     6661 ataccgtgat aaaggtaaag acggtgaact tatggccaat ctgataccaa gctaagacca
     6721 tcaaagggaa gttaatggcg tagaagctta gcgaaatcgg aatatgaaaa ccaaaccagt
     6781 gattactcaa ggcagagata atctgtgcca gacctgttgc accactcgaa tacacatgcc
     6841 ctggttggaa aaagaaatta actgctactg ctgataaaaa accatagacc agagaggccg
     6901 aaatcttctc atcatacttt tctcgagaga tactttgtaa gacacgtaaa atttttatct
     6961 gataagcaaa gcggcgcaga taatagcgcc accgcttaat tcgttttgtt tgtttcatct
     7021 tcttctactt gtaagctgag ttcctctagt tgtttgagag cgactgttga tggagcttgt
     7081 gtcattgggt cagttgcctt gttgttctta ggaaaggcaa tgacttcacg gatattttct
     7141 tctccagcaa gcaacatgac aaaacggtca agcccgatag ccaaaccacc gtgtggtggg
     7201 aaaccatagt ccatggcttc aagaaggaaa ccaaactggt cattggcttc ttcagctgag
     7261 aaaccaagag ccttgaacat gcgttcttga aggtcttttt ggttgatacg aaggctacca
     7321 ccaccaagct cataaccgtt caagacgata tcgtaagcaa tggcacgaac cttagccaaa
     7381 tcaccttcta attcgtgagc agtctcttcc tgtggaagtg tgaatggatg gtgggcgctc
     7441 atgtagcggc cttcttcttc agaccattca aacatcggcc agtcaaccac ccaaaggaag
     7501 ttgaacttat cattatcaat caagccaagc tctttagcaa tacgtccacg aagggcaccc
     7561 agtgttgcat tagccacttc aagcgtatcc gccacaaaga gaaccaagtc cttatcttca
     7621 agagcaagcg ctgttgtcaa ttcttcttgg ataccagtca agaacttggc aactggtccg
     7681 tttaattctc catcaaccac cttgacccaa gcaagacctt tggcaccata ctgtttggct
     7741 acttccgtca tcttgtcgat gtctttacgt gaatagttgt ccgcagctcc tttgaccaca
     7801 atcgctttta cagcaagtgc ttctgaaaag actttaaagt ctacaccttt gaccacttct
     7861 gtcaagtcct gaagcaacat gtcaaaacga gtatctggct tgtcagaacc gtaaagagcc
     7921 atagcatcat cgtatttcat acgagggaac ggtagcgtta cttcgatgcc ttttgtttcc
     7981 ttcatcacgc gcgcgatcaa gccttctgta atatcttgga tttcttgctc agtaaggaag
     8041 gacgtttcca agtcgacctg agtaaattca ggctggcggt ctccacgcaa gtcctcgtca
     8101 cggaaacatt taacgatttg gtagtaacgg tcaaaaccag cattcatcaa gagctgtttc
     8161 gtgatttgtg gactttgagg aagagcgtaa aaatgcccct tattaacacg agacggcact
     8221 aaataatcac gcgccccttc aggcgttgac ttagaaagga atggtgtctc cacgtcgata
     8281 aactccaact catccaagta gttgcggata gagtgggtca ccttggcacg aagtttaaga
     8341 ttttccaaca tttctggacg acgaaggtca aggtaacggt aacgcaaacg tgtatcgtca
     8401 tttgcctcaa tgccatcctt aatctcaaat ggtgttgtct tagctgtgtt aagcacaata
     8461 agagctgtca cgtttaactc aaccgcacca gttggcaact tatcattggc ttgctcacgc
     8521 gcagcgacct gaccagtcac ctcaataaca aattcgctac gaaggctttc agctgttgcc
     8581 ataacctctg cagatacttt ttcagggttg ataaccaact gcatgattcc ttcacggtca
     8641 cgaagatcga taaagatcaa accaccaagg tcacgacgac ggccaaccca tcctttcaag
     8701 gttatttctt gtccgatgtg ttcctcacga acacgaccag catacatact acgtttcatt
     8761 atttctctcc tcttttattc tgttactatt ttaccataaa agcgcaggct cttcatgaaa
     8821 atcatcagaa aagtttgcca gtctttaaaa gtcaggtgaa agccctaaaa attagcgcta
     8881 atgctcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtagg tatggtt
//
LOCUS       DAVMHG010000079         8907 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMHG010000079 DAVMHG010000000
VERSION     DAVMHG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.079"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..826
                     /locus_tag="VYR08_001431"
     CDS             17..826
                     /locus_tag="VYR08_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV3466943.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            831..1532
                     /locus_tag="VYR08_001432"
     CDS             831..1532
                     /locus_tag="VYR08_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV3466944.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1557..2474
                     /locus_tag="VYR08_001433"
     CDS             1557..2474
                     /locus_tag="VYR08_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV3466945.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2502..4043
                     /locus_tag="VYR08_001434"
     CDS             2502..4043
                     /locus_tag="VYR08_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV3466946.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4062..4520
                     /locus_tag="VYR08_001435"
     CDS             4062..4520
                     /locus_tag="VYR08_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV3466947.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPIRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4522..6744
                     /locus_tag="VYR08_001436"
     CDS             4522..6744
                     /locus_tag="VYR08_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV3466948.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6784..7893
                     /locus_tag="VYR08_001437"
     CDS             6784..7893
                     /locus_tag="VYR08_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV3466949.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8050..>8907
                     /locus_tag="VYR08_001438"
     CDS             8050..>8907
                     /locus_tag="VYR08_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3466950.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAV"
BASE COUNT         2987 a         1330 c         1786 g         2804 t
ORIGIN      
        1 caactaagga gctcatatgt tactgcaaaa agaactaatt ccaatgatag aagctaactt
       61 accaaatatg gcatatgctg aaaaagacat tgctaaattc ttcttaaaac agcaacctct
      121 gaatgattat tcatctaaag cattgtgcga ataccttaat gtatccaaag caacattgac
      181 tcgatttgcg aaaaaatgtg gttttaaagg ttttagacaa ttcattttca aataccaaga
      241 gatgattcat gagaaagaaa agttggcatt atatacagag gcaacagaaa aagttttatc
      301 cgactatgag gaaatgttga gaaaaactta cacggttctt gatgaagttc aacttgagcg
      361 tattgctgag atgatagaaa ctgctgagcg tgtatatctc tacggtaaag gaagttctgt
      421 tcttgcttta caagaaatga agatgagatt tatgcgtctc ggagtgattg gtgaagtatt
      481 atcagacgag gatatgattt tgtggagtag cttactactt aatgaaaatt gccttgtcat
      541 tggagcatcc atttcaggtc aaactgatat tgtactagaa ggtctacaaa aagctgcaga
      601 taaaggcgct aaaacagttt taatgactac aagaaaattt gacgaagaag attgtttctt
      661 tgatgaacta ttgttattag cttcgaccga tcatctctcg tatggcaatc gcatatcacc
      721 tcagtttcca atacttttaa ttacagactg cttattctct aattatctgg aaagtccaga
      781 gagacaatat tattacaatc aaactattat ccataaggag gaataataaa atgagccaaa
      841 tttggactaa agaaaaattt ataagccaag ttcaaggtgg agtcattgtt tcttgtcaag
      901 ctttacctgg tgaagccctt tataatgaag aatttagctt gatgcctttt atggctaaag
      961 cagctttaga ggcaggagca gtgggcattc gcgcaaattc tgtgcgtgat attaaagcaa
     1021 ttcagaaagt agtagattta ccaataattg gaattatcaa aagggattat ccacctcaag
     1081 aaccatatat tactgctacg atgaaagaag tagatgaact tgtagaatgc ggaacaacag
     1141 tcattgcatt tgatgcaact ttaagaccaa gatatgatgg cttagttgtc agtgaattta
     1201 tcaaaaaaat aaaagaaaaa tatccgaatc aattgctgat ggcggatgta agtaatttag
     1261 atgaaggtct ctatgcattt aaatcaggcg ttgattttgt tggtacaaca ttatcaggtt
     1321 acacaagtac aagtgtacaa tcagatgagc ctgattttga actaatgaaa aaattggctg
     1381 attttaatat tccggtaatt gccgaaggaa aaattcatta tccagaacaa ttaaaaaaag
     1441 cttatagttt aggtgttacc agtgtagtca ttggtggagc gattacacgt ccaaaagaaa
     1501 ttgctcagcg atttattaat gtcatcaaat aaaaaacaat ataaaggaga aatctgatga
     1561 aagatttaac taaatacaaa ggcgttatcc ctgcatttta tgcttgctat gatgaaaatg
     1621 gtgaaattag ccaagatcgt gtaaaatctc tggtacaata tttcattgac aaaggtgtaa
     1681 aaggtatcta tgtaaatggt tcttcaggtg aatgtattta ccaaagtgta gaagatcgta
     1741 aacaaattat tgaagctgtt atggaagttg ctaaaggtaa attaacagtt atcaaccata
     1801 ttgcatgtaa taacacgaaa gatagtatcg aattggcaaa acattcagaa agtgttggag
     1861 tcgatgctat tgcagctatc ccacctattt atttcaaatt gccagagtat tcaatcgcag
     1921 catattggaa tgcaatgagt gaagctgcgt caaatacaga ttttattatc tataatattc
     1981 cacaattggc aggggttgcg ttgactggta gtttgtatgc aacaatgcgt caaaatcctc
     2041 gtgtgattgg agttaaaaat tcttctatgc ctgtacaaga tattcaaatg tttgtagctg
     2101 caggtggaga agattacatt gtattcaatg gtccagatga acaatatctt ggtggtcgct
     2161 tgatgggagc agaagctggt attggtggta cttatggcgt tatgccagat ttgttcttga
     2221 aattggaaag tttgattcaa gaacgagatt tagatacagc taaaaaactt caatatgcta
     2281 tcaatgaagt tatctataag atgatatcag gtaaggcaaa tatgtatgct gtagcaaaag
     2341 aagttttgcg tctaaatgaa aaacttgatt taggttctgt tcgtcaacct ttagaagcat
     2401 tggcagaagg tgacttggaa gttgcaaaac aagcagcaga acttattcaa caagcacgaa
     2461 aagaattttt ataataaata aaatcaatat ggagatatgt tatgggcaca acaggattta
     2521 caataattga cttaattatc ttgattgttt atttacttgc ggtgttggtt gcaggtatct
     2581 atttctctaa aaaagagatg aaaggaaaag agttctttaa aggagatggt tcggttcctt
     2641 ggtatgttac ttcggtatcc atttttgcca caatgctcag tccgatttcc ttcttgggac
     2701 tcgctggtag ctcttatgca ggtagctgga ttttatggtt tgctcaatta gggatggtag
     2761 tagctattcc actgacaatt cgttttatct tacctatctt tgcacggata gacatcgata
     2821 cggcatatga ttacttggat aaacgtttta attctaaagc acttcgtatt atttcagcac
     2881 tcttgtttat tatttatcaa ttgggacgta tgtctatcat tatgtacctc ccatcagctg
     2941 gtttatcagt attgacagga attgacatca atattttgat tattttgatg ggtgtagttg
     3001 caattgttta ttcttatact ggtggtctaa aatccgtatt atggacagac tttattcaag
     3061 gtgtgattct gattagtggt gtcgttttag ctttatttgt actgattgct aatattaaag
     3121 gtggctttgg tgcagtagca gaaacattag caaacgggaa attccttgct gcaaatgaaa
     3181 aacttttcga tcctaacttg ctttcaaact ccatcttttt aattgtgatg ggttcaggct
     3241 ttacaatctt gtcttcctat gcttcatctc aagatttggt tcaacgtttt actacaacac
     3301 aaaatattaa gaaacttaat aagatgttgt tcacaaacgg tgttttgtca cttgcaactg
     3361 caacagtctt ttacttgatt ggtacaggct tgtacgtatt ctatcaagta caaaatgcag
     3421 atagtgcagc tagcaatatc cctcaagacc aaatctttat gtactttatt gcataccagt
     3481 taccagtagg tatcacaggt ttgatcttgg cagcgattta tgcagcatct caatcaacta
     3541 tttcaacagg tttgaactct gttgcaactt catggacatt ggatattcaa gatgtcattt
     3601 ctaaaaatat gtcagacaat cgtcgtacga aaattgcaca attcgtatct ctagcagtag
     3661 gtttattctc aattggtgtt tccattgtca tggctcactc agatattaaa tctgcatacg
     3721 aatggttcaa tagtttcatg ggacttgtac ttggtctact tggtggtgta tttattcttg
     3781 gatttgtttc taaaaaagca aataaacaag gtgcttatgc agcgctgatt gtatcaacca
     3841 tcgtcatggt atttattaaa tacttccttc ctccaacagc tgttagctac tgggcatatt
     3901 cattgatttc aatctctgta tcagtagttt caggttatat tgtatctgtt cttactggaa
     3961 ataaagtatc tgcacctaaa tatacaacga ttcatgatat tacagaaatt aaagcggatt
     4021 caagttggga agttcgtcac taatagaaaa gagaaaataa tatgattatt tctgaacaat
     4081 ctgattttaa acgatatgct tctgtcaata aatatttttc aaaagtctgt gattttttag
     4141 aaaatacaaa tttgacagat ttagttgatg gaaaaattga tattgacggg gaaaatgttt
     4201 ttgcaaattg tatgacttat ctagctgatg gagttccagg agatattttt gaaactcata
     4261 agaaatattt agatattcat attgttgttg aaaatactga aaaaatggca gtaacttccc
     4321 caattcgtgc ccagtcacgt gtacctttta gcgaagagaa ggatattgct ttttacgata
     4381 gcaaagacta tcaaattgtt gaattgcttc cagggaatat gttggtgact tttgaagaag
     4441 atcttcacca accaaagatt cattgcaatg atgaaactgt taaaaaactt gttatcaaag
     4501 ttttaaacga ggaaaaataa aatgaaaaaa aatattaaac aatatgtaac cttaggtact
     4561 gtagtggtat tatcagcatt tgttgctaac tcagttgcag ctcaggagac tgaaacttct
     4621 gaagtatcaa caccaaagtt ggtgcaacct gttgcaccaa cgactccgat ttcggaagta
     4681 caacctacat cggataactc ttcggaagtt actgtacaac ctcgaacagt tgaaactact
     4741 gttaaggatc catcttctac agcggaagaa actcctgtct tagaaaaaaa taatgttact
     4801 ttaacagggg gcggagaaaa tgttactaaa gagttaaagg ataaatttac tagcggtgac
     4861 tttactgtag tgattaagta caatcagtca agtgagaaag gcttacaagc tctgtttgga
     4921 atatctaatt ccaaacccgg tcaacaaaat agttatgtag atgtgttcct tagagacaat
     4981 ggtgagttgg ggatggaagc gcgtgatact tcttccaata aaaataacct agtatccaga
     5041 cctgcttcag tttggggtaa gtacaaacaa gaggctgtga ctaacactgt tgcagtagta
     5101 gcagattcag tcaaaaaaac atattcttta tacgcaaatg gtacaaaagt agtagaaaag
     5161 aaagtggata atttcctaaa catcaaggat attaaaggta ttgattacta tatgcttggg
     5221 ggagtgaaac gtgcaggaaa aacggcgttt ggttttaacg gaacactaga aaatatcaaa
     5281 ttctttaata gtgcattgga tgaagaaact gttaaaaaga tgacaacaaa cgctgttact
     5341 ggacatttaa tttatacggc taatgataca acaggttcta actatttccg tattccagtt
     5401 ctgtatactt ttagcaatgg tcgggtattt tcaagcattg acgctcgtta cggtggaact
     5461 catgatttct tgaataaaat taatattgct acaagttata gtgatgataa tggtaagaca
     5521 tggactaaac caaaattaac attggcattc gatgattttg cgccagtacc attagaatgg
     5581 cctcgtgaag ttggtggacg tgacttacaa atcagcggtg gtgcaaccta tattgactct
     5641 gttattgttg aaaaaaagaa caaacaagta ctcatgtttg ctgatgtgat gcctgctgga
     5701 gtaagtttta gagaagcaac tagaaaagat tcaggttata aacaaattga tggtaattat
     5761 taccttaaat taaggaaaca aggtgatact gattacaatt atactattcg tgagaatggt
     5821 actgtatacg acgatcgtac caacagacca actgaatttt cagtagataa aaatttcggt
     5881 attaaacaaa atggtaatta tttgacggta gagcaatatt cggtttcatt tgaaaataat
     5941 aaaaagactg aatatcgtaa tgggactaaa gtccatatga atatcttcta caaagatgca
     6001 ttgtttaaag tagtgccaac gaactatatt gcatatattt ctagcaatga tcatggcgaa
     6061 tcttggtctg caccaacttt attacctcct ataatgggac ttaatcggaa tgcgccatat
     6121 ttaggtcctg gacgtggaat cattgaaagc tcaactggac gtattcttat tccgtcttac
     6181 actggtaaag agtctgcgtt catttatagt gacgataatg gagcatcttg gaaagttaaa
     6241 gtagtgccac ttccttctag ttggtcagca gaagcacaat ttgtagaatt gagtccagga
     6301 gtaattcaag catatatgcg tacaaataat ggtaaaattg catatttaac aagtaaagac
     6361 gcaggtacta cttggagtgc accggaatat ttgaaatttg tttcaaatcc aagttatgga
     6421 acacaattat caatcatcaa ttatagccaa ttgattgatg gtaaaaaggc tgtcatttta
     6481 agtactccaa actccacaaa tggtcgtaaa cacggacaaa tttggattgg tctaattaat
     6541 gatgataata caattgattg gcgttatcat cacgacgttg attatagtaa ctatggatac
     6601 tcatattcaa cattgacaga gttaccaaat catgaaattg gattgatgtt tgaaaaattt
     6661 gattcatggt ctcgtaatga acttcatatg aaaaatgttg taccatatat aacatttaag
     6721 attgaagatc tgaaaaagaa ttaaagctga aatttgaaaa tatataaaaa gaggataaaa
     6781 attatggtaa attacggtat tgttggagct ggatattttg gagctgattt agctcgctca
     6841 atgaacaaaa ttgaagatgc aaaagtggtt gcggtatttg acccaaatca tggagaagaa
     6901 gttgctcaag agttgggatc agatgtttgt gcaagtttag atgaacttgt agcacgtgaa
     6961 gatattgatt gtgtgatcgt agcttcacct agctaccttc accgtgaacc agttgtgaaa
     7021 gctgctcaac atggcaaaca cgtattttgt gaaaagccaa ttgcattgtc ttatgaagat
     7081 tgtaaagcca tggttgacgc atgtaaagaa aataatgtca tctttatggc tggtcacatc
     7141 atgaacttct ttaacggtgt acaccatgct aaagaattga ttactcaagg taaaatcggt
     7201 aaagttcttt attgccatgc tgctcgtaca ggttgggaag aacaacaacc aactgtatca
     7261 tggaagaaac ttcgttctca atctggagga catttgtacc accatattca tgaattagat
     7321 tgcattcagt ttatcatggg aggacttcct gaaaaagcga caatggtagg aggcaatgta
     7381 tatcataaag gtgaaaactt tggtgatgaa gatgatatgc tcattgtaaa cttagaatac
     7441 tctgatgatc gttatgctgt tttggaatat ggtaatgctt tccgttgggg tgaacactac
     7501 gtcttgattc aaggaactga aggagctatc aaacttgact tgttcaatac tggcggtact
     7561 cttcgtgtta aaggtgaagg agaatcacac ttcttagttc atgaaactca agaggaagat
     7621 gatgatcgta cagctatcta taccggtcgt ggtatggatg gagcaattgc gtacggtaaa
     7681 ccaggagtac gttgcccatt atggttgcaa acatgtattg ataaagaaat ggaatatcta
     7741 catgacatca ttaaaggtgg agaaattaca gaagaatttg aaaaacttct caatggtgta
     7801 gctgctttag aatcaatcgc taccgctgat gcatgtactt tatcagttaa agaagatcga
     7861 aaagtaagtc tttcagaaat cacaaatgct taacttttgt aaaacagaat agtaaattct
     7921 tgtcattata taatttctaa agttctgtga tacaactcat tgaataaaga aatagagatg
     7981 ggactgggat aatgcccagt cccatttttt atcaaaaagt aatgagatca aaaatgtggg
     8041 agtgttgaaa tgaagattat aggtatcgat attggcggaa caacaattaa ggcagattta
     8101 tacgatgagt ttggaacgag tttgaatcat ttcaaagaga tagaaacaat tattgactat
     8161 gatttgggaa cgaatcagat attaaatcag gtctgtgatt taattggtga gtatacttta
     8221 aatcattcaa ttgatggtgt tgggatttcc actgctggag ttgttaatgc taatactgga
     8281 gaaatcatct atgcaggcta tacaatacca gggtatatcg gagtaaactt tactgccgaa
     8341 atagaaaaac gttttgggtt gtatactttt gttgaaaatg atgttaattg tgctgcatta
     8401 ggtgaattgt ggaagggaca agccaaagat aagaaaaatg tagtaatggt tactattgga
     8461 acaggtatag gaggcagtat tattgtcaac ggacaaattg ttaacggatt taactatact
     8521 gctggtgaag taggttatat tcctgtaggt aattcggatt ggcaaagtaa agcctcaaca
     8581 accgcattga ttcatttata tcaaaaaaag agcttgaaaa ctaatcaaac tggacgtact
     8641 ttcttcactg atttaagatc tggagataaa gttgctgaag aaacttttga aatttttgta
     8701 gaaaatctaa caaaaggttt attaacgatt tcttatctac ttaatccaga aattctcata
     8761 ttaggaggtg ggattctgga tagtaaggat attttgttac ctgaaattca aagttcttta
     8821 gctaaaaatg caatggataa taggttttta cctaaaaatc ttgtggcagc tacattagga
     8881 aatgaagctg gtcgtatagg agctgta
//
LOCUS       DAVMHG010000080         8905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMHG010000080 DAVMHG010000000
VERSION     DAVMHG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.080"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(297..1145)
                     /gene="dprA"
                     /locus_tag="VYR08_001439"
     CDS             complement(297..1145)
                     /gene="dprA"
                     /locus_tag="VYR08_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV3466951.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1241..1930)
                     /locus_tag="VYR08_001440"
     CDS             complement(1241..1930)
                     /locus_tag="VYR08_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV3466952.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1942..2820)
                     /locus_tag="VYR08_001441"
     CDS             complement(1942..2820)
                     /locus_tag="VYR08_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV3466953.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2810..3679)
                     /gene="licA"
                     /locus_tag="VYR08_001442"
     CDS             complement(2810..3679)
                     /gene="licA"
                     /locus_tag="VYR08_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV3466954.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDATSI
                     KTKFDKIAPILQTIHASGKELRGEFAPFEEIKKYESLIEEKIPYANYEAVREEVFSLE
                     KRLADLGVYRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3696..4718)
                     /locus_tag="VYR08_001443"
     CDS             complement(3696..4718)
                     /locus_tag="VYR08_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV3466955.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4723..5430)
                     /locus_tag="VYR08_001444"
     CDS             complement(4723..5430)
                     /locus_tag="VYR08_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV3466956.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5766..7253
                     /gene="tacF"
                     /locus_tag="VYR08_001445"
     CDS             5766..7253
                     /gene="tacF"
                     /locus_tag="VYR08_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV3466957.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7263..8066
                     /locus_tag="VYR08_001446"
     CDS             7263..8066
                     /locus_tag="VYR08_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3466958.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     INLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8068..8877
                     /locus_tag="VYR08_001447"
     CDS             8068..8877
                     /locus_tag="VYR08_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV3466959.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKS"
BASE COUNT         2790 a         1805 c         1502 g         2808 t
ORIGIN      
        1 cttatttcaa ttcactatac tttcccttat actcaatgaa aatcaaagcg caaactagga
       61 agcgagccac aggttgctca aaatactgtt ttgaggttgc agatggaagc tgacgtagtt
      121 tgaagagatt ttcgaagagt attatatgag aaaaatcctc tactctacta atccacttag
      181 aaaaaacgca tattatcaag tgttaaaaac ttgctaatat gcgttttatt atggaaatgt
      241 tgggttcatt ttctcttaaa attgattttt tcttagaatt ctagcttagg tcatttttaa
      301 aattcaaatt ccgcaagaac atcttgccca ctggtgacca attttgctcc ttcttgaatc
      361 aaatgatggc aaccgtctga tagtccatct aaaatgctag caggaatagc aaagacatcg
      421 cgtccttctt ccattgctcg ctcacacgta atgagactac ctgaacgcat cttagcctct
      481 gctacaatca caccacgaca aagtccagca atgatgcgat tacgggcagg aaaatgaaat
      541 ttcagaggtt gttcgccagg tccatattca cttaaaacca gatggtcatt gccgatgtag
      601 tcttgcaagc gtttattggc tttaggataa aacacatcca gtcctgttcc aatcactgca
      661 atggtttttc cgccattctg aagagctgcc atatgagctg ctgtgtcaat gcccttggcc
      721 agaccactga caataaccag ttcattttcc aagccttgaa tgactttttc aactgattta
      781 gctccctgtt tgctacaagc acgactgccc acgaccgcta ccttcgggaa tttcaggagg
      841 tcaagatttc ccttgtaaaa taaaagtaca ggcgcatcat atatttcact caaatcccaa
      901 ggataacagt catctaaaat agagaaagat ggaaattttt gaaactcttt cgacaaatgc
      961 gcatcgtcta tctgaaaata acgttccata aaaacggctg gattacggca acctgatata
     1021 tctgcaatat cacccaacaa aagctcctga tcaacatttt caccgtattc tagcactttc
     1081 aaaatctgtt gattggtcaa acctgatttt tttaacttat agatttcata gtttgtgatt
     1141 ttcataaata actccatttc tttttctact catctatcta ttcgtaaaag ttcgtaaaaa
     1201 atcaaaaaac atccgactat tttgtcagat gttggaatct ttaattttcg tttttaagaa
     1261 tttcttctaa tttacgatag tcttggacac tatcgatctc ataaatgcta ttgccttcta
     1321 attcttcaac atagacatct agctctttga tattatcctt aaccatattg tcccagtaga
     1381 gatcaacaaa ttcgccactt gcataagcct tatcgataaa gctgacaatc ttttctgcag
     1441 ttggagcatc ccagaaggat acaccactaa ggatgcgacc tgccttgcta tcaacaataa
     1501 tgtcttgaac cttgtagtca tctccataga ccaagaacca ttcgttggta caatcttcac
     1561 gataaacact aaaataagtc gaacgagtca aatcattgcg gaacatattt ttaaagagat
     1621 agttatctgc atcaataaca tagctgttgg ccaattcttc ttttacaaga tagagagagt
     1681 aaaagttatt gtagtcagcg tatttatcat tgaaaacgag acgaacaccg tatttctctt
     1741 tcaagtaatc gaattgttct ttaagataac caacaatgat gatgatgtca ttgattcctt
     1801 tttctttgag aaactcaatt tggtactcaa ttaagggttt ttgattaacc tgaaccaagg
     1861 ctttaggggt attttcagtc ataggacgca agcgagttcc caatcccgct gctaagataa
     1921 tggctttcac acgaatctcc tttattcttt aataataata taaactccag caatgacgac
     1981 aagtgacgtc ataatggtca gcatatctag cggtgcaccc aagaaaacaa ctgcaaacaa
     2041 gaccgtccat actacatagc tcacgttcaa gcctgtagcc ttggctggtt gcaagcgatt
     2101 gatagcgata taataagcca agtaggaaat catatcaaag gctgcaaaaa caatcatgag
     2161 acctagcaat tgtccattgg ctactgcagt aaatgactga tgagagaaga gcacaatcac
     2221 aagataggac aagaacgaag ttacttgacg gattaagagg gcttcgattt cactcaattc
     2281 actttccatg gcaaaagagc taagaacact ctcacttccc catgcaatag cacaaaccaa
     2341 agcacaaaga atcccaatgt agaaagaatt aacctgttca accttatagg tctgggcaat
     2401 aatccctcca ataatcaaga caatcccaaa tacagtattt ttcgaaatct tgtgcttcaa
     2461 aaagaagaaa gccaatagaa ctgaaatcgc agggtaaata gccgatacag atgaagctaa
     2521 agaacttccg atatacttaa ctgcataaag attggcctgc ataccgatag ggcctgctag
     2581 caaggctccg atgataacac tgacattgcg aatatttaag aaaattgaga ggcgaacttt
     2641 cccttctttt accaagagaa aagctagtaa gataaagatg ctcaaaaaat catgagctgc
     2701 agccaccaca aagggtgaca aatctgtaaa aatcgaaaag atataagcac taaccgttag
     2761 acccaagccc cagaaaatac ctgagagaag gccaaaagga actccgtttt tacttttcat
     2821 ctgaacctcc ataagaagcc aaacctttaa cagctctttg gtaacgattc acaccatagt
     2881 aaccaaaatc tgcaccttgc tcttccttat agacagtcca tagactccaa atagtatctt
     2941 gtaaaatttt ataaatagca atcttttcat gagaaaccgg tgtttggtca ctctcatagt
     3001 gagataagaa agtttcttcc tcttgggaag tgaattcaga ctctaaaaag agggcagcca
     3061 aatcccacat tggatcattc attgatgaat attcccagtc aatcaaataa agtcgtcctt
     3121 gaggtgattc gataaagttt tcaggcacca aatcgatatg acaagatttt ctgtaaacac
     3181 ctaagtcagc cagtcttttc tctaaggaga agacttcttc tcgaacagct tcatagttgg
     3241 cataagggat tttctcttca attaaggatt catatttttt gatttcttca aaaggagcaa
     3301 attctcctct taattccttg ccagaagcat gaatagtttg taatattgga gcaattttat
     3361 caaatttggt cttaattgaa gtcgcatcaa gcgtaatagc agattcgata tactcattta
     3421 ctttgatacc agcttcaata tcaaaaagat aattttttac atctaagcct aaatccttta
     3481 gtagttcaag attgtacttt tcatcttgtc gattgataag cttttctgtc cctttaccaa
     3541 agaatttaac aatgtattgc ttatttgttg ttttggccaa atagttttga ttggtcattc
     3601 cacccagttg ttcaacactg aggacttcct cttcttgact aagtaaggaa gaaatttttt
     3661 ctttaatgat tttctccaca attaacctcc agtacttata cttcccactt aaacactgtt
     3721 ttaaaggctg tgtttaaatc ggttgcaaag acacgatgaa tatctttaat ttctcttaca
     3781 ggttcttcta gataaaggat atttttaaga cgattggcaa atttcttgac ttccatcatt
     3841 tggatagcat tttcaaaatc aatgcgacca gaacgagatg acccaaccaa aatcaagccc
     3901 ttttctaagg catcgcgagt attgagattg actttatatt cgctaactcc catcatgaga
     3961 atcgttccct gaggacgaat gtagcgaatc aagtcattaa tagctggtcc agtaccatca
     4021 ccaccacaac attcaaaagc atggtcaaag gccaaatctt caggaatatt atccgtaata
     4081 tagcattctt tggcaaatga gaagagttcc aacttttccc aatgacgacc aataaccaca
     4141 atctctgatt ctggcaaagt atagttgata atattggcaa ccacaaaagc taaacttcca
     4201 tctccaataa cggcgatccg ctcccgcttg ctatgagcaa gagtcaatag acgattcata
     4261 gcgtgcatgc ccacactgac aaactctgta atggctgcaa ccgtatcttc aatagcatca
     4321 taagccacca cacgatcttt agggagagaa acaaactctc tcataaagcc atcaaatcca
     4381 ctagacaaga aatgggtccc tgtcatgtag ttttcataga attcttcatc actctgcata
     4441 ggagactgat tgggaatcat gacaactttt tgaccaacct cgtaggttcc ggtcgggtca
     4501 gaaatgacgg ttccacatga ctcgtgaatc attgccattg gaagcttttt attcaaaatc
     4561 ttgggatcac gttttccctg atagtaacgc tgatccgcat gacagacagc catgtagttg
     4621 ggacggataa ggatatgatt ctcttggtca atagcctctt cctgatattt gacattgata
     4681 aacttaggct tagttagttg ataaatttga ttaatcattt tactagtctt tctcaatcat
     4741 actttttgca atcttcaaat ctgttacggt tgtaatcttc agatttgagt attcaccttt
     4801 ggccaaggcc acatcttttc ctttgatcac aaagatttta catgcatctg tcaagatttc
     4861 cttctcttca tcagaaagag atccataaag gtccatgaag tccttgcaac ggaatgtttg
     4921 aggtgtttgt ccctgataga ggtgagcacg atttggaata cctgtaatga attgcccatt
     4981 ggtactttca acgatagtat ccactgcttc taccactgtg tccactgcgt catgattttg
     5041 ggcaagttgg atattgtcct gaatcatgcg aagtgtaata aatggacgaa cagaatcgtg
     5101 ggtaacaacg atatcctctg aagtaagcgg acgataagca tcaatggctt caatgatgtt
     5161 ctcaatactt gtattgcggt cagcaccacc ctttgtaatg atgatacgtt ccttataaag
     5221 aggaagatat ttatctacaa gatcttctgc atgagaaacc cagtctccat gaacaccaac
     5281 tacaattttt tcaatacttg gctccaagac aaatttttca attgtatgaa tcaaaatagg
     5341 tcgatcacct agctctaaaa attgttttgg caagttactg atccccatgc gtgtgccagt
     5401 tccaccggca agaattcctg cataaatcat ctattttctc ctttgtctta ctaaaaaaac
     5461 ttattctctc tattatacca caatctagtc ctatcataaa agaaatactg gtcctcccct
     5521 tctagaatag gagaattatg cagttctatt attcaaagaa actagtccat ttctagtaat
     5581 gactaggaat tctgtagcaa ttactaataa atgactgcga aattttagag tccttcagaa
     5641 taatatactt tccttaaaga aaacttaaaa tttaagattt gaaacttcaa agtgattgct
     5701 tggcttactg cccttcattt cttattagct aactttatga tataatgaaa aacgaggtaa
     5761 attgaatgaa aagtataaaa ttaaatgctc tatcttacat gggaattcgt gtcttgaata
     5821 ttatttttcc catcctaact ggaacctatg tcgcgcgtgt cttggaccga actgactatg
     5881 gttacttcaa ctcagtcgac actattttgt catttttctt gccctttgca acttatggtg
     5941 tctatagcta cggtttaagg gctatcagta atgtcaagga taacaaaaaa gatcttaaca
     6001 gaaccttttc tagtcttttt tatttgtgca tcgcttgtac gattttgacc actgctgtct
     6061 atatcctagc ctatcctctc ttctttactg ataatccaat cgtcaaaaag gtctaccttg
     6121 ttatggggat tcaactcatt gcccagattt tttcaatcga atgggtcaat gaagctctgg
     6181 aaaattacag ttttctcttt tacaaaactg ccttcatccg tatcctgatg ctggtctcta
     6241 ttttcttatt tgttaaaaat gaacacgata ttgttgtcta tacacttgtg atgagtttat
     6301 cgacgctgat taactacctg attagttatt tttggattaa aagagacatc aaacttgtta
     6361 agattcacct aagtgatttt aaaccactct ttctccctct gacagccatg ttagtctttg
     6421 ccaatgccaa tatgctcttc acttttttag atcgcctctt cctcgttaaa acagggattg
     6481 atgtcaacgt tagttactat accatagctc agcgaattgt gaccgttata gctggggttg
     6541 taacaggtgc aattggagtg agtgtgcctc gtctcagtta ctatctgggg aaaggagaca
     6601 aagaagccta tgtttctctg gttaatagag gtagtcgaat ctttaacttc tttatcattc
     6661 cactgagttt tggactcatg gttttaggac caaatgccat cctactttac ggtagtgaaa
     6721 aatatatcgg aggcggcatc ttgacctctc tcttcgcttt tcgtacgatt attctggcct
     6781 tagataccat tcttggttcc caaattctct ttaccaatgg ctatgaaaaa cgtatcacag
     6841 tctatacagt ctttgctggg ctactaaatt tgggcttgaa tagtctcctc tttttcaacc
     6901 atatcgtggc tcctgaatac tacttactga caactatgtt atcagagact tctctacttg
     6961 ttttctatat cattttcatc catagaaaac aactcatcca cttgggacat atctttagct
     7021 atactgttcg atactctctc ttttcacttt cctttgtagc aatttatttc ctgattaatt
     7081 tcatgtatcc tgtggatatg gtcattaatt tgccattttt gattaatact ggtttgattg
     7141 tcttgctatc agctatctct tatattagtc tacttgtctt cacaaaagat agcattttct
     7201 atgaattttt aaaccatgtc ctagccttaa aaaataaatt taaaaaatca taggagttta
     7261 aaatgaaaca actaaccgtt gaagatgcca aacaaattga attagaaatt ttggattata
     7321 ttgatactct ctgtaaaaag cacaatatca actatattat taactacggt actctgattg
     7381 gggcggttcg acatgagggc tttatccctt gggacgacga tattgatctg tccatgccta
     7441 gagaagacta ccaacgattt attaacattt ttcaaaagga aaaaagcaag tataagctcc
     7501 tatccttaga aactgataag aactacttta acaactttat caagataacc gacagtacga
     7561 ctaaaattat tgatactcga aatacaaaaa cctatgagtc tggtatcttt atcgatattt
     7621 tccctataga tcgctttgat gatcctaagg tcattgatac ttgttataaa ctggaaagct
     7681 tcaaactgct gtctttcagt aaacataaaa atattgtcta taaggatagc cttttaaaag
     7741 attggatacg aacagccttt tggttgctcc ttcgaccggt ttctcctcgt tattttgcaa
     7801 ataaaatcga gaaagaaatt caaaaatata gtcgtgaaaa tgggcagtat atggctttta
     7861 tcccttcaaa atttaaggaa aaggaagtct tcccaagtgg tacctttgat aaaacaatca
     7921 atttaccctt tgagaattta agccttcctg cacctgaaaa atttgatact attttgacac
     7981 aattttatgg agattatatg accctaccac cagaagaaaa acgcttctac agtcatgaat
     8041 ttcacgctta taaattggag gattaggatg caatatttag aaaaaaaaga aattaaagaa
     8101 attcaactag ccctgctgga ctatattgat gagacttgta agaaacatga tattccttat
     8161 tttctcagtt atggaaccat gcttggagcc atccgccaca aaggtatgat tccttgggat
     8221 gatgatattg atatttccct ttatcgtgag gattatgagc gtttactgaa gattattgaa
     8281 gaagaaaatc accctcgcta caaggttctt tcctacgata catcttcttg gtacttccat
     8341 aatttcgcat cgattttgga cacttctact gttatagaag accatgttaa gtacaagcgt
     8401 catgatacca gccttttcat cgatgtcttc ccaattgatc gatttacaga cttgagcatt
     8461 gtcgacaaga gctataagta tgtggctctt cgtcaactag cttatatcaa aaaatcacga
     8521 gcagttcacg gtgatagcaa actaaaagat tttcttagat tatgtagctg gtacgctctc
     8581 cgatttgtca atcctcgcta cttttacaag aaaattgatc aactagtcaa aaatgctgta
     8641 accaacactc ctcaatatga aggaggagtt gggatcggta aggaagggat gaaagaaatc
     8701 ttcccagttg atacctttaa agaactgatt ttaactgagt ttgagggccg tatgttgcct
     8761 gttcccaaaa aatatgacca atttttaacc cagatgtatg gcgattatat gacaccacca
     8821 tcaaaagaaa tgcaagaatg gtatagtcat agtattaaag cttatcgtaa aagctgattg
     8881 agggggatta tacaaactac taaga
//
LOCUS       DAVMHG010000081         8895 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMHG010000081 DAVMHG010000000
VERSION     DAVMHG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.081"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1284)
                     /locus_tag="VYR08_001448"
     CDS             complement(<1..1284)
                     /locus_tag="VYR08_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV3466960.1"
                     /translation="MKQRVFRGKRIRPSDKDLVFHFTVASLLPILLLVVGLFHVKTIQ
                     QVNWQDFNLSQADKIDIPYLSISFSVAILVCLLVAFLFKRYRYDTIKQLYHRQKLAKM
                     VLENKWYESEQVKTDGFFKDSPSRTKEKITYFPKIYYRLKNGLIQIQVEITLGKYQDQ
                     LLHLEKKLESGLYCELTDKELKDSYVEYTLLYDMIARRISIDEVQAHDGKLCLMKNMW
                     WEYDNLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVH
                     YRKEDLLSCIDTFYEEMIKRSEEMKQMENYKTGENYAYLGLPAHFLIFDEYVAFMEML
                     GTKENTAVINKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEM
                     GYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFY"
     gene            complement(1324..1710)
                     /locus_tag="VYR08_001449"
     CDS             complement(1324..1710)
                     /locus_tag="VYR08_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3466961.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGTVSEEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFEYNARVELINPIADTVATATFQGADVDWYIKA
                     DDIVLTKDSNSFRNQQPPKKEPATDK"
     gene            complement(1726..2040)
                     /locus_tag="VYR08_001450"
     CDS             complement(1726..2040)
                     /locus_tag="VYR08_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV3466962.1"
                     /translation="MELKFVIPKMEKTFGNLEFAGEDKVVQRRINGHLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMV
                     KE"
     gene            complement(2432..4690)
                     /locus_tag="VYR08_001451"
     CDS             complement(2432..4690)
                     /locus_tag="VYR08_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV3466963.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(4919..5149)
                     /locus_tag="VYR08_001452"
     CDS             complement(4919..5149)
                     /locus_tag="VYR08_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV3466964.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            5339..6022
                     /locus_tag="VYR08_001453"
     CDS             5339..6022
                     /locus_tag="VYR08_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3466965.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            6022..6756
                     /locus_tag="VYR08_001454"
     CDS             6022..6756
                     /locus_tag="VYR08_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3466966.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            6910..7767
                     /locus_tag="VYR08_001455"
     CDS             6910..7767
                     /locus_tag="VYR08_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV3466967.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            7909..8781
                     /locus_tag="VYR08_001456"
     CDS             7909..8781
                     /locus_tag="VYR08_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV3466968.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLFGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     ATLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
BASE COUNT         2479 a         1732 c         1893 g         2791 t
ORIGIN      
        1 ataaaactct gatatgacac ttgttcctac atcaacatag ccacgacctt tgattcgctt
       61 taagaagaaa tccttttgta cgtcactgcc aaacatcatg ccatagccca tttcagacat
      121 acgacctaaa gccactctga aattaaactg gtcacggatt ccgtcgccta gatattttgc
      181 gtctggacgc tgacaagcca gtattagaaa gaagccagct tgacgaccta acatgacaat
      241 ctgtttcaac ttatttataa ctgcggtgtt ttcttttgtt cccagcattt ccatgaaagc
      301 gacatattca tcaaagatta agaagtgtgc tggaagtcct aagtaagcat aattttcgcc
      361 agtcttataa ttttccatct gcttcatttc ctcactgcgt tttatcattt cttcatagaa
      421 tgtatcaatg caggaaagca agtcttcttt tctgtagtgg acatttgcca tcacagagcc
      481 taaatcggct aagtctgcat ttttcgggtc taagatatac agcttagaat ctgtatgaag
      541 caaggcttca atcagtgtca ggataaagta ggtcttaccg ccacctgtac caccagctat
      601 gagcatgtga ggcagattgt catattccca ccacatattt ttcattaagc aaagtttccc
      661 atcatgagct tgtacttcat caatagaaat acgacgggct atcatatcat agagcaaggt
      721 atattccacg taggaatcct ttaactcttt atccgtcagc tcacagtaca agccactttc
      781 taatttcttt tccaagtgta ggagctggtc ttgatatttc cccagagtga tttccacttg
      841 tatctgtatt aagccatttt taaggcgata atagatttta gggaagtagg ttatcttttc
      901 tttggtacga ctgggggaat ccttgaagaa gccatctgtt ttgacctgtt ctgattcata
      961 ccacttattt tcaagaacca tcttcgccag cttttgacgg tggtagagtt gtttaatcgt
     1021 atcatagcga taccgtttga atagaaacgc caccagcaag cagacaagaa ttgcgacact
     1081 gaaactgata cttaaatacg gaatgtcaat cttatctgct tgtgataggt taaagtcctg
     1141 ccagttgacc tgctggattg tcttcacatg aaacagtccg acaacaagca gtaaaatagg
     1201 cagtaaggac gctactgtaa aatgaaagac taaatctttg tcactcggac gaatcctttt
     1261 accacgaaag acacgctgtt tcatgtttga ttttctcctt tctctctaat ggaagatacg
     1321 tggctatttg tccgtagcag gttctttctt aggtggctgt tgatttctaa aggaattaga
     1381 atcctttgtc agcacaatat cgtctgcctt gatgtaccag tcaacatctg ctccttgaaa
     1441 ggtagcagta gcgacagtat ccgcaatggg attgataagt tccacccgtg cgttatattc
     1501 aaactctttc aaaggcacgc tggcaggaat acttacttga atcatgcgtc cttgcccttt
     1561 ggattttaag tcataggtac gttctttgat ttcctctgaa accgtaccgt cttcattttg
     1621 gattctcact tcacgacgta gagcagagaa ttttaattct ccaaaagtcg tgtctttatc
     1681 taatacgata ccatttgcta atctcatcat ttttcctctc tttctttatt cttttaccat
     1741 gtcgtcagca tgtaaaaggt aatttgtaaa accacgagtg ccgattttat agccctcggc
     1801 ggtaatacgt ggattgacta acttaacacg ttcctcaaag ccgaaatgtt tttcgccagc
     1861 ttcagcagga agtacgacca caatatcatc tgctctttga acgtctgaat agagattata
     1921 gcttcgagat aagacggtta gatgtccgtt gattcttcgc tgtacgactt tatcctcgcc
     1981 agcaaattct aaattgccga atgttttttc catcttggga atcacaaatt taagttccat
     2041 atttttacct atcctttctt ttttatggtt gaatgaatct cgtttgtttg atggtctttc
     2101 acggtgggga gcgaccttta ttctcttgtt tttctatttt cgtagtatca cgtcctttca
     2161 aatcgggtaa aaaaatagac acctcatttt tgaagtgtcc accttttgcc tattcaattt
     2221 ttattggaag tatctttgtt tcttcacttt tcaagggtaa atcgtcgtat caaagctcat
     2281 tcataagtag taaattagta gtaaattgat tggtttcgac cttgataaag tgtgataagt
     2341 ccagtgttta tacggataac tagattttca tactattttt agtaaaaaaa gaagcagaaa
     2401 aatttcattt tctactcctt ttctatagga tttatttttc tttttcagaa cttttaactt
     2461 cagctttttt ggcagattta atctgaatgt ttcccttgct agtcataact gctttgagat
     2521 ctttttcgct tggattttca aggtagtagt cagtgattgt gtcctcaata tagtcttgaa
     2581 tagtccgacg aagtgggcgt gctcccattt ttggatcata acctaggtca accaactttt
     2641 ccttgacctt atcagttaca tccaaacgaa tgttgttgct agagaggcgc ttgttaacat
     2701 ctgctagcat gagctcgaca atctgaagga ggttatcctt gctgagagcc ttaaattcga
     2761 taatgccatc aaaacggttc ataaactctg ggctaaagaa gttaccgagt tcaccgagga
     2821 cagaattggt acgtccttct ctagcagcac caaatccaac gctagcttcg gtctttcctg
     2881 tacctgcatt tgaggtcatg ataatgatgg catccttgaa gctaacggtg cgtccttgcc
     2941 cgtctgtcaa acgaccatcg tccaagactt gaagaaacat gtgcataaca tctgggtgag
     3001 ctttttccac ttcatcgaga aggatgagag aatatggatt gtggcgaact ttttcagtta
     3061 attgaccagc ctcatcatag ccaacataac ctggaggggc gccgaccaac ttagccacac
     3121 tatgtttttc catgtattca ctcatatcaa agcgaatcat actatcagca gaaccaaaaa
     3181 gttcgatagc cagttgtttg gaaagttctg tcttaccgac accagttggc ccaacgaaga
     3241 ggaagcttcc gattgggcgg ttaggggtac caagtccgac acgattacgg cgaatagcct
     3301 tggcaatctt atcgactgca tcatcttggc caataacatg agacttgaga tcttcggcta
     3361 gatggatgag ttgagattgt tctttctctt tcaaatcacc aacagggata ttggttttct
     3421 gctcgataat gtgctcaata gttttctcgc tgatgatagg agtatcctgg tctgtgatct
     3481 ttttcttttg catttcctta tacttggcaa tctggtcgcg gaagtaggcc gccttctcaa
     3541 aatcttcttc tcgtgtagct tgagacttga gattttcagc ctcaatcaag cgctgatcaa
     3601 ttactttagg atccacaaaa ttcaaggtca agttcatctt agaaccagct tcatctagga
     3661 ggtcaatggc cttgtcaggc aagaagcgat cttggatgta gcgattggaa agagttgcag
     3721 ctgcttcaat cgcagcatct gtatattgaa cgtggtggta atcttcgtat ttcttttgaa
     3781 tccctttgag aatagtgatt gtttcgtcca ccgttggttc atcgacttta acaggctgca
     3841 tacgacgctc gagggcagca tccttttcaa tgatacggta ttcattgagg gtagtagcac
     3901 cgactagttg cagttctcca cgagcaaggg ctggcttgag gatatttcct gcgtccatat
     3961 taccatcact cgcagaacca gcaccaacaa tttcatggat ttcatcgata aagaggatga
     4021 tgtcttcacg tttgcgaatt tcttccatga gtttttgcat gcgttcttca aattgtcctc
     4081 gaatccccgt tccttgaact aagctaacca catccagacg gatgacttgt ttaccttgga
     4141 gtttatgtgg cacatcgcca tcgacaattt tctgagctag accttcgaca acggccgttt
     4201 ttccgacacc aggttcaccg ataaggacag gattattctt ggttctacga ttgagaatct
     4261 cgatgacacg gataatctca tcgtcgcgcc caataacggg gtcaatgtct ccacgacggg
     4321 caatttcagt tacattaata ccaaattctt ccagcaggcc tttttcttgg ctaggtggcg
     4381 gagtttgagc agatccacga ttttgggaac cataaccgcc gtttccaccg taacctccac
     4441 ctgattgggt tgggggaata ggaggagtat tgctagaagg tctgaaattg tttagatcat
     4501 tgaagaaatc accaaaggga tcgaagtcac gattgttcag atccgtcata cctttgaaga
     4561 ggctattgtt aggatctgtt ttgataatct tatagcagtt ttgacagagg tcaatttgtt
     4621 tttgttttcc attgagattg gtgtaaagat gaattgttga gtcgttaatt ttacagtttt
     4681 gacaaagcat acatctacct catttctttc tttagcctga cttgtttaaa ggtcaaaaat
     4741 agtcaaattc tatactctca ttataacagg attggggtgt aaagcaagta aaaagattga
     4801 agctttgaga agttgttaca atgaaaattt ttgtgaattt ggactataaa aaaatcctat
     4861 ttgtgagaca aaaaggattt tacttagaca tgcagggaca atcccagctt gttttagatt
     4921 agtagagaag ttcgtaaatt tccattgcga ttaagtcaat gctatcaaac gtatacgttt
     4981 cgcgatcttc tacgtcacga agggtaaaga ttgggccgtt ttcttcgtcg tggctgtacg
     5041 caacttctgc aacaacaact ccttcgcgtt caaaattacg ttttaaattt ccgccatcgt
     5101 ttgccattgc ttcaaggcgg ttgatgattc taaccaaatg tgattccatt ttgattctcc
     5161 ttcatttctt atcgttacta ttttaccata aaggaggcca acttgactag aaaaaactaa
     5221 gatttctcgc taattctgct agtttaaaga tttttaaata aattggataa aaaatttaaa
     5281 ttttaattca aaacatgtta taatcataca gtattctatt ttagaaagca gtgtgactat
     5341 gaatttttct tttttaccta agtatttacc ttattttaac tatggggctg ttgtgacgat
     5401 tcttatttct atctgtgtta tctttttggg aactattttg ggtgttgtct tggcttttgg
     5461 gcaacgttca aagtttaaac cgcttgtttg gttggccaac ttgtacgttt ggattttccg
     5521 tgggacaccg atgatggttc aaattatgat tgcctttgct cttatgcata tcaatgctcc
     5581 gactattcag attggaattt taggtgttga tttttcgcgt ctgattccag ggattttgat
     5641 tatctctatg aatagtggtg cttatgtttc ggagactgtt cgtgccggaa tcaatgcggt
     5701 tccaaaaggt cagctagagg cggcttattc gctagggatt cgtcctaaaa atgcgatgcg
     5761 ttatgtgatt ttgccacaag caatcaaaaa tatcttgcca gcattgggga acgaatttat
     5821 caccattatc aaggacagct ccctcttatc agctattggg gtcatggagt tgtggaatgg
     5881 ggctacaaca gtttctacaa caacctatct acctttaaca ccgcttttat ttgcagcatt
     5941 ttattacttg attatgacct ctattctgac agtagccttg aaagcttttg aaaaacatat
     6001 gggacaagga gataagaaat aatgacagaa accttgataa aaattgaaaa tttacataaa
     6061 tcttttggaa agaatgaagt attgaagggc atcaacctcg agattaaaag aggagaagtt
     6121 gtcgttatca tcggtccttc agggagtggg aaatctacct tgcttcgctc tatgaatttg
     6181 ttggaggaag caaccaaggg gaagcttatc tttgagggag tcgatattac ggacaagaag
     6241 aatgacctgt ttgccatgcg tgagaagatg ggcatggttt tccaacaatt taacctcttt
     6301 cctaatatga ctgtgatgga aaatattacc ttgtcaccta tcaagactaa aggtgaaagc
     6361 agggaagttg ctgagaagag agcccaagag cttttggaaa aagttggttt gccagataag
     6421 gcagacgctt atccacagag tctgtcgggt gggcaacaac aacggattgc catcgcgcgt
     6481 gggttggcta tggaaccaga tgttttgctc tttgacgaac caacttcagc cttggatcct
     6541 gaaatggtag gtgaggtatt ggctgttatg caagaccttg ccaagtcagg gatgactatg
     6601 gttatcgtaa cacatgagat gggatttgcc cgtgaggtgg cagatcgtgt tatctttatg
     6661 gcagacggtg tggttgttga agatgggaca cctgagcaga tttttgaaca aacccaagaa
     6721 caacggacta aagatttctt gagtaaggtt ttataatcta ttttaaaatt tcaagacaag
     6781 attagtgaaa agttcaacca gagctttttc ttatagtttg aaactatagg ataacctagg
     6841 aaagaagtgt tagagggcac tagcagtttt tggtataatg gaagatattt gaaagaaaag
     6901 agaagtgata tgacacagat tattgatggg aaagctttag cggccaaatt gcaggggcag
     6961 ttggctgaaa agactgcaaa attaaaggaa gaaacaggtc tagtgcctgg tttggtagtg
     7021 attttggttg gggataatcc agccagccaa gtctacgttc gcaacaagga gaggtcagct
     7081 cttgcggctg gttcccgtag cgaagtagtg cgagttccag agaccattac tcaagaggaa
     7141 ttgttagacc tgattgctaa atataatcag gatccagctt ggcatgggat tttggtccag
     7201 ttgccattac caaaacatat cgatgaagag gcggttttat tagccattga tccagaaaag
     7261 gatgtggatg gtttccatcc cctaaacatg ggacgtcttt ggtctggaca tccagtcatg
     7321 attccctcga cacctgcagg aattatggaa atgtttcatg aatatgggat tgacttggaa
     7381 ggtaaaaatg cggtcgtcat cggtcgttca aatatcgttg gaaaacctat ggctcagctt
     7441 cttttggcta aaaatgcgac agtaaccttg acccactcac gaactcataa tcttgccaag
     7501 gtggctgcta aagcagatat tcttgtagtc gcaatcggcc gcgccaagtt tgtgactgct
     7561 gactttgtca aaccaggtgc ggttgtcatt gacgttggga tgaatcgaga tgaaaatggg
     7621 aagctctgtg gagatgttga ttatgaggca gttgcaccac ttgctagcca tatcacgcca
     7681 gtccctggag gtgtcggtcc tatgaccatt accatgctga tggagcaaac ttatcaagca
     7741 gcacttcgga cattggatag aaaataagag aaacattcga ttaatagagt gtattttcta
     7801 tagctatatc taaaatggca gaaatgaata ttaaatttta gatataagat tacaaaagga
     7861 ggtctgcgtc tcctttttat tgtataataa aagtgagagg aaaaaaggat gaaagtgatt
     7921 aatcaaacct tattagaaaa agttattatt gaacgttctc gttcaagtca taaaggagat
     7981 tatggtcgtc tgctgctgct ttttggtact tatccttatg gaggtgccat catcatggct
     8041 gctttagcag ctgtaaaaag cggtgcagga ttggtgaccg ttggaacgga cagggaaaat
     8101 atcccggctc tgcacagcca tttacctgag cctatggcct tttctcttca agaccagcaa
     8161 ttgttaaaag agcaattgga gaaggcagaa gttgtcttgc tggggcctgg tttacgagac
     8221 gatgcttctg gagaaaatct agtaaaacag gtctttgtta atttaagcca aaatcagatt
     8281 ttgattgtag acggaggtgc cttgaccatt cttgctagga caagtttgtc atttccctca
     8341 agccagctta tcctaactcc tcaccaaaaa gaatgggaaa aattatctgg aattacgatt
     8401 gaaaagcaaa aggaagatgc aacggctagt gttctgactt ccttccctca aggaacaatt
     8461 ttggtggaga aaggcccagc tactcgtatt tgggaagttg gccaatctga ttattaccag
     8521 ttacaggttg gtggtcctta tcaggcgact gggggaatgg gagctactct ggctgggatg
     8581 atcgcaggat ttgtaggcca attccgacag gccagtctct acgagcgtgt ggcagtagcg
     8641 acccatcttc attcagccat agcccaagaa ctatctcaag aaaattatgt ggtcttaccg
     8701 acggaaatca gcaggtatct tcccaaaata atgaaaatta tttgtcaaca agagagggta
     8761 agcaaagata aacttgttta aaatagacag ataaatctac ttccttctct taatttatga
     8821 ttcttgaaat gcgcctgctt taaggctaaa ctagacgtat caaagttaag agtttgtttt
     8881 tgtaactgga aagag
//
LOCUS       DAVMHG010000082         8823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMHG010000082 DAVMHG010000000
VERSION     DAVMHG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.082"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            153..1940
                     /locus_tag="VYR08_001457"
     CDS             153..1940
                     /locus_tag="VYR08_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV3466969.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVSRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1937..2413
                     /locus_tag="VYR08_001458"
     CDS             1937..2413
                     /locus_tag="VYR08_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV3466970.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2441..3346
                     /locus_tag="VYR08_001459"
     CDS             2441..3346
                     /locus_tag="VYR08_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV3466971.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVVVAAPSTPSESGEIEDDEF"
     gene            3333..4148
                     /locus_tag="VYR08_001460"
     CDS             3333..4148
                     /locus_tag="VYR08_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3466972.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4155..4559
                     /locus_tag="VYR08_001461"
     CDS             4155..4559
                     /locus_tag="VYR08_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3466973.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4854..6020
                     /locus_tag="VYR08_001462"
     CDS             4854..6020
                     /locus_tag="VYR08_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV3466974.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVSLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6136..7173
                     /locus_tag="VYR08_001463"
     CDS             6136..7173
                     /locus_tag="VYR08_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV3466975.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSITPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7380..8234
                     /locus_tag="VYR08_001464"
     CDS             7380..8234
                     /locus_tag="VYR08_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3466976.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEAFAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIVQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8384..8641
                     /locus_tag="VYR08_001465"
     CDS             8384..8641
                     /locus_tag="VYR08_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV3466977.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         2514 a         1660 c         2002 g         2647 t
ORIGIN      
        1 tctaaaaatg tttgaaacta attgaatgtg tgtataatag taaaaatgca gtaaatatcc
       61 cgacaatttc tattgacaat tcaaacagat tggtttataa ttaatataac aacaaatgaa
      121 agcgcaaact ttcgtggtcg gaaggtagtt ttatgacacg atttgagata cgagatgatt
      181 tctatctcga tggaaaatca tttaagattt tatctggtgc cattcattat tttagggttc
      241 ctccagagga ttggtatcat tcgctctata acttgaaggc tcttggtttt aatacggtag
      301 agacttatgt tgcttggaat ttacacgagc ctcgtgaagg tgaatttcat tttgacggtg
      361 atctggattt agagaaattt ctccaaatag cgcaggattt gggtctctac gcaattgtgc
      421 gtccgtctcc atttatctgt gcggaatggg aattcggtgg cttaccagct tggctcttga
      481 ccaagaacat gcgaattcgc tcatccgacc cagcatatat cgaggcagtt ggtcgctact
      541 atgatcagtt attgccaaga ctggtgtctc gtttgttgga caatggtggc aatattctca
      601 tgatgcaggt tgaaaatgag tatggttctt acggagaaga taaggcttac ctgagagcga
      661 ttcgacagct aatggaagag tgtggcgtaa cctgtcccct ctttacatca gatggtccat
      721 ggcgagctac tctgaaagct ggaaccttaa ttgaagagga cctctttgta acaggaaact
      781 ttggttctaa ggcaccttac aacttttcgc agatgcagga attctttgat gaacatggta
      841 agaaatggcc actcatgtgt atggagttct gggatggttg gttcaatcgc tggaaagagc
      901 cgattatcac acgggatcct aaggaattgg cagatgcagt tcgagaggtt ttggaacaag
      961 gctctatcaa tctttacatg ttccacggtg gtacaaactt tggtttcatg aatggttgct
     1021 cagctcgagg aactttggac ctgccacaag ttacatctta tgattacgat gcccttctgg
     1081 atgaagaagg aaatccaact gctaaatatc ttgcagtcaa gaagatgatg gcaacacatt
     1141 tttcagagta tccgcagttg gaaccactct acaaagagag tatggagttg gatgctattc
     1201 cactagttga aaaagtttct ttgtttgaaa ccttagatag cttgtcaagt cctgtagaaa
     1261 gtctctatcc tcaaaagatg gaggagctgg gacaaagtta tggctaccta ctttatcgaa
     1321 cagaaacaaa ctgggatgca gaagaagaaa gacttcgtat cattgatggt cgagataggg
     1381 cccagctgta tgtcgatggt cagtgggtta aaactcaata tcagacagag attggggaag
     1441 atatttttta tcaaggtaaa aagaaagggc tatctaggtt agatatcttg atagaaaata
     1501 tggggcgtgt caactatggg cataagttct tagcggatac gcaacgtaag ggaattcgga
     1561 caggggtctg taaggatctg catttcttac taaactggaa acactatcca ctcccactag
     1621 acaatcctga gaaaattgat ttttcaaaag gatggactca aggacaacca gccttttacg
     1681 cttatgactt tacagtcgaa gagccaaaag atacttacct agacttgtct gagtttggta
     1741 agggagttgc ctttgtcaat gggcagaatc taggacgttt ttggaacgtt ggcccaactc
     1801 tctcacttta tatccctcat agctatctca aggaaggtgc caaccgtatc attatctttg
     1861 aaacagaagg tcaatataaa gaagagattc atttaactcg taaacctaca ctaaaacata
     1921 taaaggggga aaacttatga caattgtagg atgccgtatt gatggacgtt tgatccacgg
     1981 acaagtagcc aatctttggg ctggaaaact aaatgtttca cgcattatgg ttgtagacga
     2041 cgaagttgtc aacaacgata ttgaaaagag tggtttgaaa cttgcgacac caccaggtgt
     2101 gaaattgagt attttgccag ttgagaaagc tgcagccaat attcttgctg gtaaatacga
     2161 tagccaacgt ctctttatcg tggctcgtaa accagaccgc ttccttggtt tggtagaagc
     2221 aggtgtacca cttgaaaccc ttaatgttgg gaatatgtct caaacaccag aaactcgttc
     2281 tattacacgt tctatcaacg tagtagacaa ggatgtggaa gacttccaca aactggcaga
     2341 aaaaggtgtt aaacttactg ctcagatggt tccaaatgat ccaatttcag actttttgag
     2401 cttattaaaa taggaaaaaa atttttagga ggtcattgtt atgatacaat ggtggcaaat
     2461 tttacttctc actttgtact cagcttatca aatctgtgat gagttgacga tcgtttcatc
     2521 tgcaggttct cctgtatttg ctggtttcat tactggttta atcatgggag atgtgactac
     2581 tggtttactt atcggtggta acttgcaact gttcgttctt ggggttggta ccttcggtgg
     2641 tgcttctcgt atcgacgcaa cttctggtgc ggttcttgcg acagccttct ctgtttcaca
     2701 aggaattgat gcaccgcttg ccattactac aatcgctgta ccagtagcag ctctcttgac
     2761 ttacttcgac gttcttggtc gtatgactac taccttcttc gctcaccgtg tggatgctgc
     2821 aatcgaacgc tttgactata aaggtattga acgcaactac ttgcttggtg cgattccgtg
     2881 ggctctatct cgtgcccttc cagtcttctt tgcccttgct tttggtggtg cctttgtaca
     2941 atcagtagta gacttcgttg aagcctacaa atgggttgca gatggcttga cactcgcagg
     3001 acgtatgctt ccaggtcttg gatttgcaat cttgcttcgt taccttccag ttaaacgtaa
     3061 ccttcactac cttgctatgg gatttggttt gacagctatg ttgactgttc tttactcata
     3121 tgtaacaggt cttggtggcg ctgttgctgg tatcgtaggt actcttcctg ctgaagttgc
     3181 tgaaaaaatt ggtttcgtga acaacttcaa aggtttgtct atgattggta tttctatcgt
     3241 aggtattttc cttgcagtgc ttcacttcaa aaatagccaa aaagtagttg tagcagcacc
     3301 ttctacacca tcagaaagtg gggaaatcga agatgacgaa ttctaattac aaacttacaa
     3361 aagaagattt taatcaaatc aacaaacgta gcttgtttac tttccaatta ggttggaact
     3421 acgaacgtat gcaagcttct ggttaccttt acatgatctt gcctcagttg cgtaaaatgt
     3481 atggtgatgg aactcctgaa ttgaaagaaa tgatgaaagt tcatactcaa ttcttcaata
     3541 cttcgccatt cttccatacc attatcgctg gttttgacct tgccatggaa gaaaaagatg
     3601 gtgtaggttc aaaagacgcc gttaacggta tcaagacagg tttgatggga ccattcgctc
     3661 ctcttgggga tacaatcttt ggttcacttg tacctgctat catggggtca gtcgcagcaa
     3721 ctatggctat cgctggccaa ccttggggga tcttcctttg gattgcagtt gcagtagcgt
     3781 atgacatctt ccgttggaaa cagttggaat ttgcttacaa agaaggggtt aaccttatca
     3841 acaacatgca aagtaccttg acagctttga ttgacgctgc atctgtactt ggtgtcttca
     3901 tgatgggtgc tcttgtagca acagtgatta actttgaaat ttcttacaag ttgccaatcg
     3961 gtgaaaagat gattgatttc caagacatct tgaaccaaat cttcccacgt ttgcttccag
     4021 caatctttac tgcctttatc ttctggttgc ttggtaagaa aggtatgaac tctactaaag
     4081 ctatcggtat tattatcgta cttgctttgg ctctttctgc ccttggtcac tttgcacttg
     4141 gaatgtaatt ccttatgact aaatcattaa ttttggtgag ccatggtcgc ttctgtgagg
     4201 agcttagagg tagcacagaa atgattatgg gcccacaaga caacatttac acagtagctc
     4261 ttcttccaga agatggccca gaagaattta ctgctaaatt tgaagctgtt attgaaggat
     4321 tggatgattt cctagttttt gcggatcttc tcggtgggac accttgtaat gtggtgagtc
     4381 gcttgatcat ggaaggtcgt gatattgacc tttacgcagg gatgaatctt ccaatggtga
     4441 ttgaatttat caatgcgagc cttacaggcg cagatgcgga ctacaagagc cgtgctgcag
     4501 aaagcattgt gaaagttaat gacctgttag cgggcttcga tgatgacgaa gatgaataat
     4561 actcttcgaa aatctcttca aactacgtca acgtcgcctt gccgtaggta tatgttactg
     4621 acttcgtcag tcttatccgg caacctcaaa acggtgtttt gagctgactt cgtcagtctt
     4681 atccggcaac ctcaaagcag tgctttgagc agcctgcggc tagtttccta ttttagttgg
     4741 aactcgattc aattcatgtg acaacgtgaa aatcgttaga gcattttata tagaatatac
     4801 atgggaatgg agcttactcc cattcccata tttaatagaa aaagaggaac tcaatgctac
     4861 attatacaaa agaagacttg ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc
     4921 aacagcctga tgtatggaga gaagcttttg aattttatca agcaaaacgt gaagaaattg
     4981 cagccttcct acaagaaatc gctgataaac atgactatat taaggttatc ttgacaggtg
     5041 ctgggacttc tgcttatgtg ggagatacct tgctacctta ttttaaggaa gtctatgacg
     5101 aacgcaaatg gaatttcaat gctattgcga caacagatat cgttgccaat ccagcaacct
     5161 atttgaaaaa agatgtggca actgtccttg tgtcttttgc tcgtagtggg aactcgcctg
     5221 aaagtgtggc gactgttgat ttggccaaat ccttggtgga tgagctttat caagtgacga
     5281 ttacttgtgc agcagatggt aaattggctc ttcaagctca cggtgatgac cgtaatctct
     5341 tgctcttgca accagctgtc tctaatgatg ctggatttgc catgacttct agctttacgt
     5401 ctatgatgtt gacaactctc ttggtctttg atcctacaga atttgctgtt aaatctgaac
     5461 gttttgaagt tgtatctagt cttgcccgta aagttttaga caaggcagaa gatgtcaaag
     5521 agctcgttga tttagacttt aaccgtgtca tctatctagg cgctggtcct ttctttggac
     5581 ttgctcatga agctcagctc aagattttgg aattaactgc tggtcaagtt gcgaccatgt
     5641 atgaaagccc agttggcttc cgtcacggtc caaaatctct tatcaacgac aatacagttg
     5701 ttttggtctt tggtacaacg acagactata ctcgtaagta cgacttggac ttggttcgtg
     5761 aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc
     5821 ttgaaaatgt caaagaagtg tcccttggtt gtggcggtgt cttgaatgat atttaccgtg
     5881 tcttccctta catcgtttat gcccaactct ttgccctatt gacttcactc aaggtagaaa
     5941 ataaaccaga tacaccgtct cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa
     6001 ttcacgaata tcaaaagtaa gacagtgttt atgaattctt gacaagagga tttgtaaatt
     6061 atcagataaa ccatagattg tcagtacgct ttctatggtt tgtttgcttg agagaaatag
     6121 taaaaggaga acagaatgaa agcatacaca gagcgtgtat ttggaaatgt tgagggcgag
     6181 gatgtcttgg cctatcgatt tgagacagac ggtggctacc aacttgaggt tatgacttat
     6241 ggcgcgacca tcttgcgcta tgtcgcacct gacaaggctg gaaattttgc caatgttatc
     6301 ttgggatttg atgactttga tagttatgta ggcaatagtc ccaagcatgg agcaagtgta
     6361 ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc tcaatggtaa gacctatgac
     6421 cttgaggtta ataatgctag caactgtaat cacagtggtt caactggttg ggattccagc
     6481 ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc tctacacaga acgtacagat
     6541 gggacaggag ggttccctgg aaatctcaag atttggatca gttatcactt ggaagaaact
     6601 ggtgcctatg aaattagcta caaggtaacg accgatcagg atacgctggt caatccaacc
     6661 aaccacagct atttcaactt gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc
     6721 caactaaaca cagagggcat ttactcaatc actcctgacg gtgttcctgc caaaactcca
     6781 gaagccaacc gtgatgtggt caaacacatc tacaatggta ccttgttgaa ggatatcttt
     6841 gcagaagaag atgagcaaat ccagctggca tcaggtttgg atcacccatt tgcccttcct
     6901 gtaggccatg acaatgctgg attcctttat gaccaaaatt caggtcgctt cctgcttttc
     6961 aagacagaag ctccttgctt tgtggtctac acagcaaact ttgtggatga aagtgtcatc
     7021 ataggaggtc agccaatgct acagcacaat gggattgctc ttgaagcgca agctttacca
     7081 gatgccattc acagtgacct taaaggccaa gtcattctta aagctggtca aaccttcacc
     7141 agtaagacac gttatgaact tgttgtgaaa taaaaaagct ggtcaaaaag tctttaaaat
     7201 taaaataacg catattgtca ggtgatgaaa agtttgatac tatgcgtttt attatgaaat
     7261 gatttacttt atcttatcct gaaatggtgt tatttttgag ttatctggct tgactttctt
     7321 gatggaaatt atataatagt tgtaataaca ataattacaa cgagaaaaga ggaccattta
     7381 tgacctatga atacaagagt cacatttatt tggcagagac agttttaaat gtaaaggatt
     7441 tggccagtca aacagccttt tatcagcaag ttattggttt agaaatctta tctcaaactg
     7501 agacagagtc cattctaggc cttggtggaa aagtcttggt acagctgatt caagcacaag
     7561 agagcggaga agtgagggaa cattatggtc tttaccattt ggccattctc ttgcccacac
     7621 gcaaggcttt ggctgatgtc ttgaaacacc tgacagattt acagattcct cttgtcggcg
     7681 gtgcagacca tggttacagt gaagctattt acttggaaga tttggaggga aatggcattg
     7741 aactctatcg agataagcca gtttccacat gggatattcg agaagatgga cgcattatcg
     7801 gagtgacgga ggcttttgcg gcgcaggaca tttatgagtt gggggaaaga gtagagccct
     7861 ttatcctggc agaaggtacg agaatggggc atattcatct ttccgtcaag gatagtcgaa
     7921 agtccagcca gttttatcaa aaggtgttag ggctagagga taaattcagt gtgcctagcg
     7981 ctagttggat tgcggctgga gattaccatc atcatttagc agttaacgaa tggggaggaa
     8041 aaggtctggc ttcgcgtaag caaggcttac caggtttagc ctactatgtc atcgaagtcg
     8101 cacataaaga agaactgtta acgattgtcc aacgagcaca agaagttgac gcaccaatca
     8161 aatggatgac atcgagccaa ttggaaatca cagactcaga tggcatcgtg acccgtattc
     8221 gtttagcaag gtaaaattag gaatttctaa taagttataa gtattgctag tttgcttttt
     8281 gaacggtctt tggtaacaga actgcgtgag ctggatgggt gagaattttg acaaagaagc
     8341 tgattagaga tataatgaag aaaaatcgag caaggaaaaa gaaatgacaa attcaaagta
     8401 tattacttgt ttgaaacgtt cagagggcca gttgtgtggg attcaaaaga tgattgaagg
     8461 agatcgtgac tgtgctgata ttgtgacgca actgacagca gtgagatcta gtgtagagcg
     8521 cgtgattgag atgataatta ccgaaaatct tactgaatgt atcaatcagc cactagatga
     8581 ttctgaagct caaaaggaac gcctagaaaa ggctatccga tacttgatta aacggaaata
     8641 aatgaatgtt ttgagacgat atgtgcaaag gtctattaac tattagaata gtttaatgtg
     8701 agtcaaaaga agttgtaaaa aatctttaaa attaaaaaaa cgcatagtat caggtgtttt
     8761 attatgtaga gacttatact cttcgaaaat ctcttcaaac cacatcagct tcaccttgcc
     8821 gta
//
LOCUS       DAVMHG010000083         8797 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMHG010000083 DAVMHG010000000
VERSION     DAVMHG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.083"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..771
                     /locus_tag="VYR08_001466"
     CDS             <1..771
                     /locus_tag="VYR08_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3466978.1"
                     /translation="ILISIFPIAWIFLSSLKADPMKNPGISLPTDFTLEGYINVFTKL
                     HVFTYFWNSFKVVSISVIISIVMISMSSYVIARMEFRGKKLVTSMLYSTLFIPATAMT
                     FPVYRLVNELGIYNTPVALILVYSCSGIAMSFFIIKNYFEIIPKELEEAAEIDGATYA
                     QTFWKVMLPIARPGILTAAVLAFINNWNEYYWASMLVIDKNELTVPALLGQFTTSFNT
                     NYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGAVKG"
     gene            768..2087
                     /locus_tag="VYR08_001467"
     CDS             768..2087
                     /locus_tag="VYR08_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV3466979.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            2093..3964
                     /locus_tag="VYR08_001468"
     CDS             2093..3964
                     /locus_tag="VYR08_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV3466980.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIENVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYFGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKYLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            3986..6202
                     /locus_tag="VYR08_001469"
     CDS             3986..6202
                     /locus_tag="VYR08_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV3466981.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLGTEKILDLAKAAKDLEIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADKIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSRRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            6213..>8797
                     /locus_tag="VYR08_001470"
     CDS             6213..>8797
                     /locus_tag="VYR08_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV3466982.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETV
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISDQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKV"
BASE COUNT         3039 a         1149 c         1777 g         2832 t
ORIGIN      
        1 attttaattt ctattttccc tattgcgtgg atttttttat catcattaaa agcagatcct
       61 atgaaaaatc caggtattag tttaccgact gactttactc ttgaaggtta tataaatgtt
      121 tttacaaaac ttcatgtttt cacttacttt tggaatagct ttaaagtagt gtctatatca
      181 gttattatta gcattgttat gatctccatg tcttcatatg ttattgctag gatggaattt
      241 agagggaaga agttggtgac ttctatgctg tattctactc tatttattcc agcaacagct
      301 atgacttttc cagtttatag attagtaaat gagttgggga tttataatac cccagttgct
      361 ctaattctgg tctactcttg tagtggaatt gctatgagtt tctttattat aaagaactat
      421 tttgaaatta ttccaaaaga attggaggag gcagcagaga ttgatggtgc gacatatgct
      481 caaacttttt ggaaagtgat gttgccaatt gctagaccag gaattttaac agcagcagta
      541 cttgccttta ttaataactg gaatgaatat tattgggcat ctatgttggt gattgataaa
      601 aatgaattaa cagtaccagc attactaggt caatttacta caagttttaa tactaattat
      661 aatggtctat tttcagctat tgttgtaatc gtattaccac caatcattct attcgcattt
      721 acaagtaaat acttcattga agctttagga ggaggagcag taaaaggatg aaacatacac
      781 ttgaaactat caatagtaga actcagtggt ttagagaagc aagatttggg atgttcattc
      841 attgggggtt atattctatt ccaggaaaag gggaatggat tcgtagtcat cagaaattat
      901 ctattgaaga ttatgaacca tattttcgag cttttgatcc taaggaatat aaccctagag
      961 aatgggctaa acaagcaaaa gctgcaggga tgaagtatat ggttttaacg gctaaacatc
     1021 atgatgggtt ttgtttgttt gattcaaaat ttacagatta taaagcaaca aatacacctg
     1081 caggtagaga tttagttaaa gaatttgtag atgctgttcg agctgaaggt ttaaaagtag
     1141 gcttatattt tagtttgatt gactggcacc atcctgattt ccctaaatat gctgatctaa
     1201 atcatccaat gagaggaaat gaagcctatc gagatgagaa aattaacttt gacagttatt
     1261 tggagtattt gcacaatcaa gttaaagaaa ttgtaactgg ttatggtcag attgacatat
     1321 tatggtttga ttattcttat gaagacatgg tgggtgaaaa atggggagct tcaaaattaa
     1381 tagacatggt tcgtcattat cagccaaacg ttatcgtaga taatagatta gaaacatcag
     1441 gtgaaggatt cggaagtatc gttacagatg aaattacctc ttacgctgga gattttgtta
     1501 gtccagagca aattgttcct cacgaaggta ttcgaaactt taaaggtgaa cctgttcctt
     1561 gggaactttg tctaactatg aataacaatt gggcttataa tcctacagat tatctatata
     1621 agtctagtca aactttgata agaaagttag tggaatgtgt tagtaaaaac ggaaatatga
     1681 ttcttaatgt aggcccagat gctttgggaa gaattaatga ttcaagtaag aaaatattag
     1741 ataattttca tcgatggatg tctcgaaatg gtgaggcaat ttatggatgt agtggagatg
     1801 aaaatttacc aaaaccagat tggggatact atactcgtaa tggaaatacg gtttatgctc
     1861 atgtatttga acaacctata ggtcctttag ctcttcttgg tattagtaaa gaaaacgtaa
     1921 aaagaatgag ctttcttcat gatggaagtg aggtgaaaat ttcagaaagc tggacgacca
     1981 atgcctacaa aggaatttgt tttgcacagt ttggagaagt tccacatttt acctatccat
     2041 taccagactt gattgatagc gtaatcaaaa ttgagttgag agagtagttt atatgaaaat
     2101 tgaaaataaa aatgtaagac gtaatttctt ttggggagat ggtcgatttt atactactga
     2161 tatcgtaaac aaacgggcag gagtaatgat agaaaatgtt tcgaaagaag aatttaccat
     2221 tacattggag aatggtataa gactttcttc aaatcatttt tcagcaatcg ttagggaaga
     2281 gggagatacg cgaattcagg tttcttttgt ttgtccttcc attcgtttac gtttaatctt
     2341 tgaatcgagg gatgatgttc tttcgaagca attagtttta gaatcatcta ctgaggttat
     2401 taaatctgta gaggtagaga gttttgagtt tgaaacagaa gacaatattt tctatccgaa
     2461 aagacaagat tgtattaagg aaatggcgaa tttttccggt tattatgtag aattgggaca
     2521 acctgtttat gcgaattctt tattcttagg aatggaattt ccaatgtctg aaaacaaggt
     2581 agatggtaga cattatgtat caagatatta ctttggaact gttgtaaatc aagaaaagag
     2641 tttgtggtct tgtattattg ggggagcatg ttcttataaa aaagaagaga ttcaagaggc
     2701 attttttgaa tatgttgaag gaatagctca acctagttat ttccgtaaac agtataattc
     2761 ctggtatgac catatgaccg atattacaga ggaaggtatt ttaaaaagtt tttctgagat
     2821 tcgagatgga tttgaaaatc atggagttca tttagatgct tatgttgttg atgatggttg
     2881 gacaaactat caatcagttt gggaattcaa tcataaattc ccaaatggtt tgagaaatat
     2941 taaatatctt gtaaatggat ttggttcaag cctaggattg tggattggtc cccgaggtgg
     3001 ttataatggg acagaaatca ttatgagtga ttggttagaa gcacatccag agttaaatat
     3061 tggatctaaa aatttgattt caaatgatgt aaacgtggct gattttaact atctcaatca
     3121 aatgaagaaa aagatgttgg aatatcaaaa agaattcgat atcagctatt ggaaaattga
     3181 tggttggtta cttcaacctg acaaacctga taagagtgga ccgcacggta tgtataccat
     3241 gacagcggtt tatgagttct taattcaact gttgatagat ctaagaaagg agagaggagg
     3301 aaaagattgt tggttaaact tgacttctta tgtaaatcct agtccatggt ttttacagtg
     3361 ggtcaatagt ttatggattc aaatatctca agatgtaggc tttacagaga atgcaggtaa
     3421 tgatatcaat cgtatgatta cttaccgaga tattcagtat caagaatttt tggaaaaacg
     3481 tgagatacag ttacctatgt ggtcgcttta taatcatgaa ccaatctatg ctgtcagtgc
     3541 aaatacctgg tacatggatc atcaaatgtt tgcatcaata ccagattttg aagcttatct
     3601 attatttatt tcaacaagag ggaatgcttt ttgggagttt cactattctt ttgatatgtt
     3661 tgatgaagaa cgctggaaag ccaatgctcg tgccgtcaaa tggatagagg agaattatca
     3721 aacactaaaa tatagtaaaa aaataggagg aagtcctgaa aaatttgaaa tttatggtta
     3781 taagtgtcac aatcagaaga cttctactga aattctctct ctgagaaatc ctgctcagat
     3841 taaacaaaaa ataaaaatag agaatctttc gatagagaat tttaccagag taattggaga
     3901 ttttactatt caagaagatg agatagaatt ggcaccgtac tctatcgtga tactgaagaa
     3961 gtaaagaaaa aattgtgagg tagagatgac gatttatatt aataaggacg agaccgtttt
     4021 tcatttggca atgaaagata gtagttatat ttttagaatt ttagaaaatg gagaacttca
     4081 acatctacat tttgggaaaa ggattcatgt caaggaaaat tataatcaat tgatggccta
     4141 tgaaaaaaga ggatttgaag tatctttttc tgaagaattt gaggatattc aacagtctat
     4201 gatacaaaat gaatattctt catatgggaa aggagatttt cggcatccag cctttcaagt
     4261 tcaaggaatg aatggtagta ggataacgac actaaaatat caaggttttg aacttgaaaa
     4321 agggaaaaat cgtcttaact ctctaccttc aacatttgat gatattggtc agtgtgcgga
     4381 aacattaacg attattttaa cagattccat attagattta actgttagac taaattacac
     4441 aatttttccg gaatacaatg tcttagttag aaatacggaa tttttaaata atagcaataa
     4501 taagttgact cttttgaaag caatgagctt acagctagat ctacctgata gtcaatatga
     4561 ctttattcaa ttttctggag catggctgag ggaacgtcag ttatatagaa cttcgcttag
     4621 accaggtatt caagcaatag atagcttgag atactcatca agtcctcagc aaaatccttt
     4681 ctttatgcta tcaaggaggg aaactacaga gcatagtggt gaggtttatg gttttaactt
     4741 tatctattct ggaaattttc aaaatatgat tgaagttgac cattttgaca ccgctagagt
     4801 aacggtagga ataaatccag tagaatttcg ttttttatta aatcctgccg aaagttttgt
     4861 gacaccagaa gcaattgtga tctattctga tcaagggatg aatcagatga gccaacaact
     4921 atcagatttt tatcgacatc atttagttaa tcctaatttt tctcaagcta gtcgtcctat
     4981 aatactcaat agttgggaaa cattttattt tgacttgggt acagaaaaaa ttttagattt
     5041 agcaaaggct gctaaagatt tagagataga attatttgta ctggatgatg gttggtttgg
     5101 tcataggaaa gatgacaaaa gttctctggg ggattgggta acagatagaa gtcgccttcc
     5161 tgaaggtatt ggatttcttg cagataaaat tcacaaaata ggtttacaat ttggtttgtg
     5221 gtttgagcct gaaatgattt ctattgatag tgatttgtac aagaatcatg ccgattggac
     5281 tatccatttg ttagacagag agaagtcagt aggaagaaat caatatgtgt tggatttgac
     5341 gagacaggaa gttgttgatt acctttttga ttctatttct aaaatcataa tcaaaacaaa
     5401 tctggattat atcaaatggg atatgaatcg tcatataaca gatatttata gtattgaact
     5461 tgattctgaa cagcagatgg aatttggtca tcgatatatc ttaggtcttt atcagctatt
     5521 agatcgttta ataactaagt tcccttcagt tctatttgaa tcttgttctt caggtggtgg
     5581 acgttttgat ttaggactta tgtattatgc accgcaagcg tggacgagtg atgatacgga
     5641 cccgatagaa agattgaaaa ttcagcatgg aacttcttat ggatattctc catcaatgat
     5701 gacagcccat gtttctattt ctccaaatga acaaagtaga agacaaacga gtttggacac
     5761 taggacaaat gtagcttatt ttagttcttt cggttatgaa ttagatgtta cgagattgtc
     5821 ggtagaagaa aaagaacaag tgagagaaca aattcagttt tataaaaaat atcgttcatt
     5881 gtttcaatat ggggatttct ataggataaa cagtcctttt agttgtgatt ctgctagttg
     5941 gcaagttgtt tcaaaagata aatgccaatc gattttattg tatgctcaat tgaatagtaa
     6001 gttgaatcca ggttatacaa gagtttattt tagtggttta gataaagata aatgctattc
     6061 cgtctctgga tttgatgagt tcttttatgg agatgaatta atgaatgctg gaataaaagt
     6121 cagtttaagt aatttagcac tttgtgttcc agaatatctt acaaaattat ttgttattga
     6181 ggaagttgta tgtaaatatt gaggaaataa aaatggaaag taaaaaaata gcaaaacaga
     6241 ttttaatagc tacggctgtt ttaacaagtt ttttaggaag taatttggtt tatgcagatg
     6301 tagttcaaag taattctaat aacagagcta gtactgaaac tgttagagta acagggaata
     6361 acttagaaaa acttattact aaagataaag aaattgataa agagatgaca tatctttcag
     6421 atatggattg gtcttctgca actcatggtg atatagataa aacaaaaaca gtacaaaaag
     6481 atgctccatt tacaacagga aataaaggag agcatactaa gatttcattg ctcacttctg
     6541 atgataaagt taaatatttt gataaaggaa taggaacagt cgcagattct ccatcagtaa
     6601 tcagctatga tattagcgat caagggtttg aaaaatttga aacttatata ggaattgatc
     6661 aaagtgctaa tagttctaga tcagaccatg cagtagtgga tagaatagaa atagaaattg
     6721 atggaaaggt tgtctattct agtagtgtaa ctaatccaga aggattcaga tataataccc
     6781 aggcacaatt tattagtgtg acaattccac aaaacgctaa aaaaatatcc ttaaaatcat
     6841 tcgcaggaga acatacatgg ggtgatgaag ttgtatttgc tgatgctaaa cttattaaaa
     6901 ctgttagtac tcaaactata actccagatc ttcttaataa aggaattaat ggaggagtat
     6961 atctttctga tttagaatgg gttgatgcta ctcatggtga tgacgataaa tcaaaaacag
     7021 ttcagaaaga taaaccgttt acacctggta ataacgggtc taataataaa ataaaattac
     7081 taattgacgg taaggaagtc gaatttaata agggattggg aactgtagca agtaacccgt
     7141 ctagtataaa atatgatgtt tcgggtgcta atgttacaag atttatttcc tatgtcggaa
     7201 ttgataggag tgcaaatcat ttaaattcag attacgcaga tattcaaaaa tttgaagttg
     7261 ttgcggatgg taaagtcatt tactcatcag attcaaaata tccaaaagga attaagtatg
     7321 atacatcggc atttctggtt gatgttgaaa ttcccaaaga tacacagaca atagaactta
     7381 aatcatattc agggaaacat acatgggctg acgaattggt tttaggtgga gccttgttta
     7441 tggcaaatgg taaatttaag aatcctaatg attggtcaga agttgataaa cgtcgtgaaa
     7501 ttaataatga acatccgtta cttatgatgc ctttatatgc taacggagaa gagtttaacc
     7561 aagggaaata tactttttgg ggaggagata cattaacagg taagtgggag aatataccag
     7621 atgacttaaa accatatact gttatacagt tacatccaga tgatcttcct aaaagagatg
     7681 gagctgctag agatttttat gaacatatgt tagaagaagc agcgaaatat gtaaatccta
     7741 agactggaaa aaatgaacca attccagtta ttctgacggt ttacactgct ggtaatatgc
     7801 cttattatac ttcggcacat tggttaagta ccagctggat tgataaaatg tatcaaaaat
     7861 atccaaattt gcatggtatt ttcagtacag agaattattg gatttgggca aatgatattg
     7921 aaaataaggc ggcagattac ttaaaagtat cagctaaaaa tggaggatat tttatatggg
     7981 ctgaacaaaa taatggttct gcaatagaaa aagcatttgg aaaaaacggt aagatagcat
     8041 ttcagaaatc tgttgataag tactggaaga accttatttt catgttcaaa aatacacctg
     8101 ctgctgaggg aaatgattca actacagaaa gttatatgaa gggactttgg ctttcaaatc
     8161 atacttatca atggggtggt ttgatggata cttggaagtg gtatgagaca ggtaaatgga
     8221 aactgtttgc aagtggtaat atcggaaaaa gtcaaggaga tagacaatgg ttgactgaac
     8281 cagagtctat gctaggagaa gaagctttag gggtttacct taatggaggt gtagtttata
     8341 attttgaaca cccagcttat acctatggtg taaataataa agaaagctta ctctttagcg
     8401 aagtaataaa agaatttttt agatatgtaa tagcgcatcc agcaccatcc aaagaaaagg
     8461 tacttgagga tacaaaagta ttcatacatg gagactattc aaataaagga aatggtaagt
     8521 tctttgtaaa cgtaaatact gatagagaac aaactccatt atacatgact ggtcgttata
     8581 atgttattcc agctattcca ggtgttttaa aaacagataa attgaaagag tctgtttctg
     8641 gtagccgtat tcagattaag gaaatcactt ctccagaatt tagctctact caggctagaa
     8701 aagaatactt aaataagctg tatcctatga attatgaagg agatatcttt gctcagaaat
     8761 tagataatag atggtttgtc tataattaca aggtaaa
//
LOCUS       DAVMHG010000084         8673 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMHG010000084 DAVMHG010000000
VERSION     DAVMHG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.084"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..334
                     /locus_tag="VYR08_001471"
     CDS             <1..334
                     /locus_tag="VYR08_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV3466983.1"
                     /translation="VNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQIPRK
                     SSKLKPLIAEDKAYNHALSKDRSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAG
                     IINHELGF"
     gene            371..847
                     /locus_tag="VYR08_001472"
     CDS             371..847
                     /locus_tag="VYR08_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV3466984.1"
                     /translation="MPYFIGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPHPFPETG
                     MLDFQDRSPWLEDQKELDLNYDLFSTDAVTLDELQSRTIALRSRKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFIAFEPWSGLSTSLEEGDHLEDKKNVRLLEANQVEEIGFEIEVL"
     gene            1004..1429
                     /gene="lacA"
                     /locus_tag="VYR08_001473"
     CDS             1004..1429
                     /gene="lacA"
                     /locus_tag="VYR08_001473"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV3466985.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHVVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            1460..1975
                     /gene="lacB"
                     /locus_tag="VYR08_001474"
     CDS             1460..1975
                     /gene="lacB"
                     /locus_tag="VYR08_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV3466986.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHVESHNAHQADANFFTEF
                     LEKWDRGEYHD"
     gene            1986..2915
                     /locus_tag="VYR08_001475"
     CDS             1986..2915
                     /locus_tag="VYR08_001475"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV3466987.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVENIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGAFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKITGHVNMANYQALYDQLIVKEV"
     gene            2917..3897
                     /gene="lacD"
                     /locus_tag="VYR08_001476"
     CDS             2917..3897
                     /gene="lacD"
                     /locus_tag="VYR08_001476"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV3466988.1"
                     /translation="MALTEQKRARLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4131..4967
                     /locus_tag="VYR08_001477"
     CDS             4131..4967
                     /locus_tag="VYR08_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3466989.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGITFGKKKGDLIAEHQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALAQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVEVVKSIDKRKEILRNVEEVLKDYEIQRTKENNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5003..5320
                     /locus_tag="VYR08_001478"
     CDS             5003..5320
                     /locus_tag="VYR08_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3466990.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFAKADALVE
                     EAGNCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5320..7011
                     /locus_tag="VYR08_001479"
     CDS             5320..7011
                     /locus_tag="VYR08_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV3466991.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGTSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            7226..8632
                     /gene="lacG"
                     /locus_tag="VYR08_001480"
     CDS             7226..8632
                     /gene="lacG"
                     /locus_tag="VYR08_001480"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV3466992.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKLAETQVID"
BASE COUNT         2734 a         1567 c         1806 g         2566 t
ORIGIN      
        1 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
       61 tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca
      121 aattccacgt aaatccagca aactcaagcc gctaatagct gaagataaag cctataacca
      181 tgcgctatct aaggatagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa
      241 aatgttttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgag
      301 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ataaagaaac
      361 tagcaaggtc atgccttact ttattggtgg ccatccagga tttaattgtc ctctactgga
      421 tgatgaggtc tatgaagatt attatctaga gtttgagaaa gaagagactt gctctgttcc
      481 acatcctttc ccagaaacag gtatgttgga tttccaagat agaagtccat ggctagagga
      541 tcaaaaagaa ttagatctta attatgatct tttcagcacg gatgcagtca ccttagatga
      601 attgcaatct cgaacaattg cgcttcgttc tcgtaaacat gataagggat tgaaagtgca
      661 ctttgcagag tttccaaatc ttatcatctg gtcaactttg aataaaggtc ctttcattgc
      721 ttttgaacca tggtctggct tgtcaacatc ccttgaagaa ggagatcatt tagaagataa
      781 gaagaatgtt cgtctcttag aagccaatca ggttgaagaa atagggtttg agatagaagt
      841 tttataaaaa agtaaaaaat aaataacaaa aacaaacaca aactgttgac tttttcaaac
      901 aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac aggaaataca
      961 ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta ttgttatcgg
     1021 tgcagatgct gcaggtttga gattgaaaga agtggtgaaa gacttcttgg aaaaagaaaa
     1081 cttccacgtt gtggatgtta cagctgaagg tcaagacttt gttgatgtga ctcttgctgt
     1141 tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg cttatggggc
     1201 tggtcctttc atggttgcaa caaaaatcaa aggaatggtt gctgcagaag tttctgatga
     1261 acgttcagct tatatgactc gtggccacaa caactcacgt atgattacta tgggtgcaca
     1321 acttgtcggt gatgaattgg ctaaaaatat cgctaaagga tttgttaacg gtaaatacga
     1381 cggtggccgt caccaaattc gggtcgatat gttgaacaaa atgtgctaat tagattacag
     1441 taagaaaggt aagttaaaaa tgagaattgc aattggatgt gaccacatcg taacagatga
     1501 aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg actttggtac
     1561 atatgaccat acacgtactc actacccaat ctttggtaaa aaagttggtg aagctgtaac
     1621 tagcggtcaa gctgatcttg gggtatgtat ctgtggtact ggtgttggta tcaacaacgc
     1681 tgtaaataaa gtcccaggtg tccgttctgc cttggttcgt gatatgacaa cagcgcttta
     1741 tgctaaagaa caattgaacg ctaacgttat cggttttggt ggtaagatta ctggtgaatt
     1801 gctcatgtgc gatatcatcg aagctttcat ccatgctgaa tacaaaccat ctgaagaaaa
     1861 caagaaattg attgcgaaaa ttgaacatgt ggaaagtcac aatgcacatc aagctgacgc
     1921 aaacttcttt acagaattcc tagaaaaatg ggatcgtgga gaataccacg actaagaggt
     1981 gacctatgat tttaacagtc acaatgaacc catctattga tatttcctat cccttggatg
     2041 agttaaagat tgatactgtc aatcgtgtgg tggatgtaac caaaacggct ggtggtaagg
     2101 gactcaatgt tacccgagta ctttcagaat ttggagattc tgttcttgct actggtttag
     2161 tgggtggcaa acttggtgag tttttagttg aaaatatcga tgatcaagtt aagaaagatt
     2221 tcttctcaat tcagggagaa actcgtaact gtattgctat tcttcatgga gataaccaaa
     2281 cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac tttttggaac
     2341 atttcaaaaa actcttggag tcagttgaag tagtcgctat ctcaggtagt ctgccagctg
     2401 gccttccagt tgattactat gcgagcttgg tagaacttgc taatcaagct ggcaagcctg
     2461 ttgtcttgga ctgctctggt gcagcacttc aggcggttct tgaatcacca cataaaccaa
     2521 cagtcatcaa accaaataat gaggaattgt ctcaacttct aggaagagaa gtttctgagg
     2581 atttggatga attaaaagaa gtccttcaag aacctttgtt tgcaggtatt gaatggatta
     2641 tcgtttcact tggtgccaat ggtgcctttg ccaaacatgg tgacactttc tacaaggtag
     2701 atattcctag aattcaggta gtcaatcctg ttggatctgg agattctact gtggcaggaa
     2761 tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcatcaag gcaaatgtcc
     2821 ttggtatgct caatgctcaa gaaaaaataa ctggtcatgt caacatggcc aactatcaag
     2881 ctctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga acaaaaacgt
     2941 gcacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc atttgaccaa
     3001 cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac agtggctcaa
     3061 atggaagaac tgaaagtttt ggtagcagat gaattgacta aatacgcttc atctatgctt
     3121 cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc tggtcttctc
     3181 cttgcttatg aaaaaacagg ttacgacaca acaagcacaa aacgcttgcc agactgcttg
     3241 gatgtttggt ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa attcttgctt
     3301 tactatgatg tagatagctc agacgaactc aaccaagaaa aacaagctta catcgaacgt
     3361 atcggttctg aatgtgtggc tgaagatatc ccattcttcc ttgaaatcct tgcttacgat
     3421 gaaaaaattg ctgacgcagg ttctgtagaa tacgctaaag taaaaccaca caaagttatc
     3481 ggtgctatga aagtcttctc agacccacgc tttaacattg atgtcttgaa agtagaagtt
     3541 cctgttaaca ttaaatatgt tgaaggcttc gctgaaggtg aagtggttta tacacgtgaa
     3601 gaagcagcag ccttcttcaa agcacaagat gaagcaacta acttgccata catctactta
     3661 agtgcaggtg tatcagctaa actcttccaa gatacccttg tatttgccca tgaatcaggt
     3721 gcaaacttta acggagttct ttgtggtcgt gctacatggg ctggatcagt tgaagcttac
     3781 atcaaagatg gtgaagcagc agctcgcgaa tggcttcgaa caactggatt tgaaaatatt
     3841 gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg cgtgtaagaa
     3901 agtcctccta ggttaggaac atgaatctaa aaatttttaa aaaaagttgt atgtaaaggt
     3961 ttacaaaaca acttacttgt gctatactta aatcacaagt taatacaagg tgagtgttac
     4021 taagtaagat taggcatgat cacagatgaa ttagaaatcg gctgattttc tagttcattt
     4081 gtggtcattt tttgtactca tataccttta agatataaaa ggaggttgac atgtatcgaa
     4141 ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaagga gaagaagtga
     4201 ttgtgattgg taaagggatt acatttggaa agaagaaggg ggatttgatt gctgaacatc
     4261 aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt atggctctcc
     4321 tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat aagctatcaa
     4381 agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat catatttact
     4441 gttcttacca agctctagca caaggaaggt acaaggatag taatctgcca gatatttctg
     4501 ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac cgtcagaagc
     4561 tagcagatca ttttcctgag gatgaaatta ttcggattgc ttatcatttc atcaatgccg
     4621 aaggcgagaa tgaagtagaa gtagttaagt caattgataa gaggaaagaa attctcagga
     4681 atgttgaaga agttttaaag gactatgaaa ttcaacgaac caaagagaat aaccatttct
     4741 atgatcgctt catgatccat ttgaattatt tcttggatta tttagacaga tctagggatg
     4801 ataaccaatc gcttcttgat atggaagatc atatcaaaca atcctatcca aaagccttcg
     4861 aaattggctc caaaatctat gatgtgatta cgcaacatac gggtcttgat ttgtataaaa
     4921 gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa tttaattaaa
     4981 attatataag gagaattcta tcatgaatag agaagaagta acattgttag gttttgaaat
     5041 cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttaaagg ctgctgaagc
     5101 tggtgatttt gccaaagcgg acgctctggt agaagaagct ggtaactgta tcgcagaggc
     5161 tcaccatgcc caaacaagtc tattgactaa ggaagcttct ggtgaggatt tagcatatag
     5221 tgtgaccatg atgcatggcc aagaccactt gatgacaacc atcttgttaa aagatttgat
     5281 gcaccattta atcgaacttt ataagagagg agttaaataa tgaacaaact aattgcattt
     5341 atcgagaaag gaaagccttt ctttgaaaaa ctatctcgta atatctatct tcgtgctatt
     5401 cgtgatggtt ttattgctgg tatgcctgtt attctcttct caagtatctt tatcttgatt
     5461 gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc ttttctgatg
     5521 aaaccttaca gctattcaat ggggattcta gctctcttgg tagctggtac aacagctaag
     5581 tcattgaccg actcagtaaa ccgtagcatg gagaaaacca atcaaatcaa ctatatgtca
     5641 acattgttgg cagcaattgt tggtttgttg atgttggcag ctgatcctat cgaaaatggt
     5701 ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc tgcctttgtt
     5761 accgtagcaa tctataaggt ttgtgttaag aacaacgtca ctattcgcat gcctgacgaa
     5821 gttccaccaa atatctcgca agtctttaaa gatgtgattc cattcactct ctcagttgtt
     5881 tcactttatg ctctcgattt actagctcgt cattttgttg gtacaagcgt agctgaatca
     5941 atcggtaaat tctttgcacc attattctct gctgctgatg gttatctagg tattaccatt
     6001 atctttggtg cctttgcctt cttctggttt gttggtattc atggtccatc tatcgttgaa
     6061 ccagcaatcg cagctattac ctatgcaaat gctgaagtca acttgaacct tctccaacaa
     6121 ggtatgcacg ctgacaagat tcttacttct ggtacacaaa tgtttatcgt taccatgggt
     6181 ggtactggtg cgacactggt tgttccattc atgttcatgt ggttaacaaa atcaaaacgt
     6241 aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa tgaaccaatc
     6301 ttgtttggtg caccacttgt attgaatcca atcttcttta ttccatttat ctttgctcca
     6361 attgcaaacg tttggatctt taaattcttt attgaaactc ttggaatgaa ttcattcact
     6421 gctaatctac catggacaac tccagctcca ctgggtctag ttcttggtac gaacttccaa
     6481 gtgctatcat tcattcttgc tgcccttcta atcgtggttg acgtagtcat ttactatcca
     6541 ttccttaaag tctatgatga acaaattctt gaagaagaac gttcaggtaa gtctaatgat
     6601 gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc tattcttgag
     6661 aaagcgggtg tagaagcagc acaaaataca atcactgaag aaacaaatgt cctcgttctc
     6721 tgtgcaggtg gagggacaag tggtctcctt gcaaatgctt tgaacaaggc agcagcagaa
     6781 tacaatgtcc ctgtgaaagc agcagcaggt ggctatggtg ctcaccgtga aatgttgcca
     6841 gagtttgatc ttgttatcct tgcccctcaa gttgcttcaa actttgaaga tatgaaggca
     6901 gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata catcaaatta
     6961 actcgtgatg gaaaaggtgc cctagcattc gtacaagagc aattcgatta aggctagaga
     7021 ctctgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta atcgccttat
     7081 taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattca gtgacttggc
     7141 acaagaagtg agatggagaa gcatggctca ctgactcctc tcctctcact tttactttat
     7201 ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact ttattttcgg
     7261 tggcgcaaca gctgcttatc aagcagaagg tgctacacat actgatggaa aaggaccagt
     7321 tgcttgggat aaatatcttg aggataacta ctggtacact gccgaaccag ctagtgattt
     7381 ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca atggtattcg
     7441 aatttctatt gcttggtcac gtattttccc gactggttac ggccaagtaa atgctaaagg
     7501 tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt
     7561 aactcttcat cactttgaca ctccagaagc tctccactca aatggagact tcttaaaccg
     7621 tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat ttccagaagt
     7681 aaactattgg acaaccttta atgaaattgg accaatcggt gatggtcaat atttggttgg
     7741 gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac accacaatat
     7801 gatggtgtct catgcacgcg cggtaaaatt gtacaaagag aaaggctata aaggggaaat
     7861 tggtgttgtt cacgccctgc caactaaata tcctctagat cctgaaaatc cagcagatgt
     7921 tcgtgcagct gagttggaag atatcatcca caataaattc atcttagacg caacttatct
     7981 aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag tcaatggtgg
     8041 tagtttggat cttcgtgaag aagattttac agcattagaa gctgcaaaag acttgaatga
     8101 tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg gagaaactga
     8161 aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca aaggtgttgg
     8221 tcgtcgtgta gctcctgact atgtaccacg cacggattgg gattggatta tctaccctca
     8281 aggtttgtat gaccaaatca tgcgcgtgaa gaaagattat cctaactata agaaaatcta
     8341 catcactgaa aatggtcttg gatataaaga tgagttcgtt gataacactg tttacgatga
     8401 tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga ttgcagatgg
     8461 agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat ggtcaaatgg
     8521 gtatgagaaa cgttacggtc tcttctatgt agactttgaa actcaagaac gttatccgaa
     8581 gaaatcagct cactggtaca agaaactagc ggaaactcaa gtgatagact agtagaatga
     8641 gtcattagat atagaatttt agtgaggcaa aaa
//
LOCUS       DAVMHG010000085         8672 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMHG010000085 DAVMHG010000000
VERSION     DAVMHG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.085"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            298..1170
                     /locus_tag="VYR08_001481"
     CDS             298..1170
                     /locus_tag="VYR08_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV3466993.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFNSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1316..1660)
                     /locus_tag="VYR08_001482"
                     /pseudo
     CDS             complement(1316..1660)
                     /locus_tag="VYR08_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1803..2114
                     /locus_tag="VYR08_001483"
     CDS             1803..2114
                     /locus_tag="VYR08_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3466994.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            2118..2834
                     /locus_tag="VYR08_001484"
     CDS             2118..2834
                     /locus_tag="VYR08_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3466995.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYSASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(2964..3779)
                     /locus_tag="VYR08_001485"
     CDS             complement(2964..3779)
                     /locus_tag="VYR08_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV3466996.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLIIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(3776..4681)
                     /locus_tag="VYR08_001486"
     CDS             complement(3776..4681)
                     /locus_tag="VYR08_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV3466997.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGWAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            5093..7222
                     /locus_tag="VYR08_001487"
     CDS             5093..7222
                     /locus_tag="VYR08_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV3466998.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            7209..7658
                     /locus_tag="VYR08_001488"
     CDS             7209..7658
                     /locus_tag="VYR08_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV3466999.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            7716..7988
                     /locus_tag="VYR08_001489"
     CDS             7716..7988
                     /locus_tag="VYR08_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV3467000.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(8342..8533)
                     /locus_tag="VYR08_001490"
     CDS             complement(8342..8533)
                     /locus_tag="VYR08_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467001.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         2734 a         1565 c         1830 g         2543 t
ORIGIN      
        1 tactatattt ttcgcctcta acaatcgcaa tctcttcttt atctacgaat gaaatcgctt
       61 tctatttttg taagtaaagc atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt
      121 ttcgtaaaaa atttgttgac agagcacgaa acgcctacta taattaaatc aagcttaagg
      181 aaataacaaa aaaacaaatc ggagggaaag acgatgaaca cattaaatga gaaagtaatc
      241 aatatctgta aagcagtagt taaagaaact ttaatccaag acatttagga gaaagaaatg
      301 aagaaatcaa gaaaattagc cacattagga atctgttcag ccttgttttt aggcttggct
      361 gcttgtcaac aacaacatgc tacttctgag gggacgaatc aaaggcaaag cagttcagcg
      421 aaagttccat ggaaagcttc atacaccaac ctaaacaacc aggtaagtac agaagaggtc
      481 aaatctctct tatcagctca cttggatcca aatagtgttg atgcattttt taatctcgtt
      541 aatgactata ataccattgt cggctcaact ggcttatcag gagatttcac ttcctttact
      601 cacaccgaat acgatgttga gaaaatcagt catctctgga atcaaaagaa gggcgatttc
      661 gttgggacca actgccgtat caatagttat tgtcttttga aaaattcagt caccattcca
      721 aagcttgaaa agaatgacca attgcttttc ctagataatg atgcgattga taaaggaaag
      781 gtctttaatt cacaagataa ggaagagttt gatattctat tttcgagagt tccaactgag
      841 gcaactacag atgtcaaggt tcacgctgaa aagatggaaa cattcttctc acaatttcaa
      901 ttcaatgaaa aagctcgaat gctgtctgta gtcttgcacg acaatttgga tggcgagtat
      961 ctgtttgtag gccacgttgg ggtcttagta cctgctgatg acggtttctt atttgtagag
     1021 aaattgactt tcgaagagcc ctaccaagcg attaaatttg ctagtaagga agattgctac
     1081 aagtatttgg gcaccaagta tgcggattat acaggcgagg gactggctaa gccttttatc
     1141 atggataatg ataagtgggt taaactttaa tagtgttatg atatgacagt aatcttagga
     1201 tttttatttc tatttttgat gacttggtgg gttatttcaa tcgttaatag ccagaaacca
     1261 ctcaaaaatg aaacatttat tggctatgtc caaagatacg atattgatgg gaatggctat
     1321 caatcattac cgtgtcctca gaactgagag gagttcttga aatcgtaaaa ccactttgaa
     1381 caagagttac ttcaacccat tggctctgat ggattaagtt gctttcgtga ataccaaaat
     1441 cagccgcaat ttgttcataa gtgcggtatt ctcgcacata ttgaagagtg gccataagaa
     1501 ggtcttctag gcttaattta ggttttcgtc caccttttgc gtgtttaagt tgataagctg
     1561 tttttaatac agctaatatc tcttcaaaag tcgtgcgctg aacaccaaca agacatttaa
     1621 atcgtgcatc agttagttgt ttacttgctt cataattcat agaactacta taccatattt
     1681 tgtttcgcag gaagtctaat gctaaaatct tatcagtgag aagatttctt aaagtttatt
     1741 ctttgtagtt tcatctgtat tagtacataa tttaacttag agaaaatagg aggactaatc
     1801 gaatggaatt aaaagatttt acagaaaaag aacaggaaat gattaagaaa aggcttacaa
     1861 tgtctaatat tagtgacaaa gaaactactg agaagattct ggcgctagta ccacaagact
     1921 tgattaagcg aatcccgttt tttgtcagaa aacatgctac aacacgtacg attaaacgca
     1981 tttcaattga acaccctgaa ctctacgctg tagctcaaac aagtggtgag attccggaaa
     2041 aagaacgcga agaattgcgt cagattatca cgactatctt tgaacaaaag atgaataagc
     2101 atagtattaa gtagagaatg aaaaaatatt ttattagcgg tttgggaagc aatgcctatc
     2161 atagcaagga tttttttcaa gaactagatt cgcaggtcta ttttctaaat ccatatgaaa
     2221 agcatcttcg agatgaaaca gaattgaaat catggtttaa aaatgagatt gtagaggaag
     2281 aatctatctg tctgataggt cattctcttg gaggagattt agctcgttat tccgcatcgg
     2341 aatttgaaga agtaaagaaa ctgattcttt tggatggtgg ctatctagat ttagataaga
     2401 ttttaccttt ggatacagag ttagaggaaa ctaaaaatta tatcaaatct caaattgttt
     2461 cggacttagc tcttcttact tctaaagaaa aatctgaagc aaagcattgg tcagaaaata
     2521 tggagaaagc tgtaagacag tcctatcact ggaatgttga gtataataga tatgagttgg
     2581 ctataaatta tgaaaatata gaagcgatac tccgcctacg gagaaaaata caagctttta
     2641 agagagaagt gggagatacc ttgtttatta gtcctcgcta tcctaatgaa gctacatgga
     2701 gagaggaagc cctaaaagaa ttgccagact attttgatac tattttttta gaaaactttg
     2761 gccatgagct ttatactcaa gcacctaaag aaatcgctag tctaatgaat gagtggctcg
     2821 cttattttct atgagctact tgtattgctg gaaatggtat aagatgaaaa tctacaagtc
     2881 actaattttc atataaccaa aaaacgaaca ggctcttttt ctgttattca gaagagaact
     2941 tgttcgtttt tatcttgatt ttcttaaaaa gctctatata catagggatt ttctggttta
     3001 tcgactttgg taaagctgtc gatttccaag gttggaaggt tttgtttgag ttctttatgg
     3061 tagtgattga ccagttttcc tttggctgaa atccaagtgt tgttttcata ctgccgggta
     3121 ttgcccttgg tcagcaatcc atagacacca gaatctgcga tacagtggat aataccgaat
     3181 cggaacagaa attgactatt ggcatgactg gggtcgttat agacaaagcc tgtaaactgg
     3241 attgtcttgc cctcaaactc atctggatag tcgtagatag cctccatgac ttccatatag
     3301 ttttcattag tgatctgaat actatcttgg gataagtatt tatccgccgc cgttcgcatt
     3361 tccttttcat aggctgattt tgaaaaataa gaactggtat ctggtttcaa atattggctt
     3421 gtcgtccctt cgcttgtctg aatggctaga tccgttcctt ctgatagggg gaaatgataa
     3481 cctttagcag aaacggtctg agaatccaag ctaacagttg ggaaagttaa gccgatgata
     3541 agtggaatag ccagaagaga aatactcgtc atcttggcca atcggctgtt cagatgactg
     3601 tgggttttga cttgcttcat ccagatatac aattgaacaa ttgccaagat aaaggaaagc
     3661 accatggaga tataggccag ataggaatag tgcatgttga tgtactggtt tagtttgccc
     3721 gacagatgga ggtaaatagt cagttcaaaa tagccagcta aaactaaaaa tcgaatcata
     3781 ggatgactcc aatcaagaga gaataggcta aaacgaccag agtcacaatc gtaatgaagt
     3841 gactaataaa tcgtgctttc aagtaatttt tcatcatgag aatatttttg atatccagca
     3901 ttgggccaat gacgagaaag gccagaactg gtgcccaacc gaaactcgag agtagagaag
     3961 cacctataaa ggcatccgcc tcactacaga gcgaaagaag aaaggccaaa agcatcaaga
     4021 gcaggatggc aaaaagagga gtcgcactga tagaggtcag aatccgagtc ggaacgtaga
     4081 cctgtattat agaagcaaag aggcagccaa ataccaaata acgccccgta tcaaaaaatt
     4141 catcaatggc ctgcacaaag acttgaaaaa ctttttttgc agaactcaag taagaaaaat
     4201 catgctcatg acaagccaga cgattttctt tctgaatcgg ttcttgccag aaaaatccta
     4261 gaaaaattcc tagtattaca gccacaagaa tggaacccag agctcgtaat agggcgacat
     4321 ggaaggagtt gccaaaggca gaataggtcg caaaaagaac aatgggattg ataacaggtg
     4381 ctgtcacaag aaaaggaacg gccgtgtaac ttggaacctt tttttccaga aaacgattga
     4441 tgatggggac gattccacat tcacaagaag ggaaaagtat accgacaaag gtcccaaaaa
     4501 agattctccc ccaacgattt cgagggagaa aatgataaac cttgtcaggt gtgatataaa
     4561 cttcaatcag ccctgagaca atgcttccta tcagaacgaa gggaagggct tcaatgataa
     4621 tggagagaaa aatggctcca gtttgaagta cactagatgg gagttgttga aaagcagtca
     4681 tctagttttt cttttctgct ttgacttttt tctctttgtc atcagggaaa gtaacttgtt
     4741 tcaaatcagg gagtttagca aactcttcaa acatcttgtc caagtcgtct cgttttgtaa
     4801 atttgacagc cataggtgta cctcgattct aaattctacc tctattatac tattattttg
     4861 cggaaaaggg agttttttaa tttgttttcg atagttgata cacttcgaaa atctcttcaa
     4921 accacgtcag cttcgcctta ccgtagtacg gttactgact tcgtcagttc tatccacaac
     4981 ctcaaaacag tattttgagc agcctacggc tagcttccta gtttgctttt tgattttcat
     5041 tgagtatgaa aaggattgga gaaagccttc cagcttgtgg tataatagat ttatggataa
     5101 aaaatatgaa aaaatctctc aggatttggg agtgacgtta aagcaaattg ataccgttct
     5161 aagtttgaca gctgaagggg cgactattcc ctttatcgcg cgttatcgca aggacatgac
     5221 tggtagtctg gatgaggtgg cgattaaggc tattattgat ttggataaaa gtctgacaaa
     5281 tctcaatgac cgtaaggaag ctgtcttagc taagattcaa gaacaaggta agttgaccaa
     5341 ggaattggaa gaagctatct tagttgccga aaaattagca gacgttgaag aactctatct
     5401 tccttataag gaaaagcgtc gtaccaaggc aaccattgcc cgtgaagctg gactctttcc
     5461 tcttgctcgc ttgattttgc agaatatagt tgacttagag aaagaagctg aaaagttcgt
     5521 ctgtgaagga tttgcgactg gcaaggaagc cttgaccggt gcagttgata ttttggtcga
     5581 agccttatcg gaagatgtga ccttgcgttc tatgacttat caggaagtgc tgagacactc
     5641 taaacttact tctcaagcca aggatgaaag tcttgatgaa aagcaggttt ttcagattta
     5701 ttatgatttt tcagagacag ttggaactat gcaaggctat cgtaccttgg ctctcaatcg
     5761 tggggagaaa cttggtgtct tgaagatcgg ttttgaacat gcgacggacc gtattcttgc
     5821 cttctttgct actcgtttca aggtgaaaaa tgcttatatt gatgaagttg ttcagcaatc
     5881 cgttaagaaa aaggtcttgc ctgctatcga gcgtcgtatt cggacagaat taactgagaa
     5941 agctgaagaa ggagctatcc aacttttttc tgacaatctg cgcaatctcc tcttggttgc
     6001 tccactgaaa gggcgcgtgg ttcttggatt tgacccagcc tttcgtacag gtgccaagct
     6061 agctgtcgtg gatgcaacag gaaaaatgct gacaactcag gttatttatc ctgttaaacc
     6121 agcatcagct cgtcaaatcg aagaagccaa gaaagattta gcagatttaa ttggtcaata
     6181 cggtgtagag attattgcca ttggaaatgg aacggccagt cgtgaaagtg aagcttttgt
     6241 agcggaagtt ctgaaagatt tccctgaagt cagctatgtt atcgttaatg aaagtggtgc
     6301 ttctgtctat tctgccagcg aacttgctcg tcaggagttt ccagacttga ccgttgaaaa
     6361 acgctctgcc atttctatcg cccgtcgttt gcaagatcct cttgcggaat tggtcaaaat
     6421 cgatcctaag tcaattggtg tcggtcaata ccaacacgat gtcagtcaga agaaactatc
     6481 tgagagtctg gactttgttg tcgatacagt ggttaaccaa gttggtgtca atgtcaatac
     6541 agctagccca gctcttcttt cacacgtagc tggactcaac aaaactatct ctgaaaatat
     6601 tgtcaaatac cgcgaggaag aaggaaaaat cacttcacgc gcccaaatca agaaagttcc
     6661 tcgtctggga gccaaggcct ttgagcaggc tgctggtttc cttcgtatcc ctgaaagtag
     6721 caatatcctt gataatacag gagttcaccc agagaattac actgcagtta aggaactctt
     6781 caaacgttta gatattaaag acctaaacga agaagcccaa agcaaactca agtcgctttc
     6841 agtcaaggaa atggcgcaag agttagacct tggtccagaa actcttaaag atatcattgc
     6901 agatcttctc aaaccaggtc gagatttccg tgattccttt gacgcacctg tgcttcgcca
     6961 agatgtccta gatatcaagg acttagtggt tggacaaaag ctagaaggag tggtgcgtaa
     7021 cgttgttgat ttcggtgctt ttgttgatat cggaattcat gaggacggtc tcatccatat
     7081 ttcccatatg agtcgcaagt ttatcaaaca ccctagtcaa gtggtggcag ttggagattt
     7141 ggtgagcgtt tgggttaaga aaattgatac tgaacgtgaa aaagttaatc tgtctctcct
     7201 agctccaaat gaaactgact gattacgtca agcaggtttc actagaagac ttcggcagac
     7261 cttttatcca tcatgtccag tggaataggc gtctacgttc gacaggtggg cgatttttcc
     7321 ccaaagatgg gcatttggat tttaatccca aggtttatca ggaactgggg ttggacgttt
     7381 ttaggaaaat tgtccgacat gaactctgtc attatcacct ctattttcaa gggaaaggct
     7441 atcaacacaa ggatcgggat tttaaggaac tgttgaaagc agtggatgga ttacggtttg
     7501 taccatcctt gcccaatagt aactccaagc caatcaagct ctatcgttgc caatcctgcc
     7561 agcaaaggta tcagcgtaag cgtaggattg atacccaacg ctatcgctgt ggactttgtc
     7621 gaggaaagtt gctattagta aatcagcctg aggactgatg aaagccgagc ctatccgtga
     7681 tatactagtc taacctagca gaaagaggaa atatcatgaa cagtaagttt tataaaatga
     7741 gacgaaatcg catgatttca ggagttttag caggtctgtc agacaagtgg aactttgatg
     7801 ttacccttgt acgttttctg tttgccattt ttacagtcgc aaactttgga cttggggtga
     7861 ttatctatat catcctagct tctatcatgc caaccaagga agaaatcgaa gcagaaatgt
     7921 acggaacagg cccacgtaaa cgcaaagaag ctcaagccat tgacgataat gaaggctggt
     7981 tttggtgagg ttttacaaaa gatttggaat acgcaaaaag ctttaaatca atagtctaaa
     8041 ctgcttgatt taaagctttt ttacttttat actcaataaa aatcaaagtg caaactagga
     8101 agccagccgc aggctgtaat ttagtcggca atgtgaagtt gatatagttt gcactttgat
     8161 ttttgaatag tattaataat atttcgccag cttcatccac aaactatagt ggattggaac
     8221 tagaatagga cacctatact tctaaaacat tgttagaaat cgatttgact gtcctgatcg
     8281 atttgtcctg ttcttatttc attttactat aaaagcagta atttaaacgc agttttttaa
     8341 tctatttttg ctgttgagac aatacatttt tggaaaaatg gtggatgctg ataacaactg
     8401 ataaccagca aataacataa gcagtgccgg taaattctgt actgataaag tgtgtgtgtt
     8461 caactaagag ctgaccgatg aatggagaga gcagatagag aagtccgatg agtatatgtt
     8521 ctttgtgttt cattaaagcg caccttcttt ccttataggt tgaacgactt gcaagtgtta
     8581 aattgaatgg tatgttatct aggtaagctt actaaaatgt tagttcccgc ttcctccttt
     8641 tcaaagtaag ccttttcaca cttatatttt ac
//
LOCUS       DAVMHG010000086         8664 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMHG010000086 DAVMHG010000000
VERSION     DAVMHG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.086"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..426
                     /locus_tag="VYR08_001491"
     CDS             <1..426
                     /locus_tag="VYR08_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV3467002.1"
                     /translation="RYINKKYLDLPETFENYNVFVPEANGSGALGEVLSTPLIGEPLI
                     GHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVTQHNSRKTWYYVPLQDFTV
                     NSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            591..3845
                     /locus_tag="VYR08_001492"
     CDS             591..3845
                     /locus_tag="VYR08_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3467003.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGNIQKLVDSYFENN"
     gene            complement(4231..5217)
                     /locus_tag="VYR08_001493"
     CDS             complement(4231..5217)
                     /locus_tag="VYR08_001493"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV3467004.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5411..7879
                     /gene="gyrA"
                     /locus_tag="VYR08_001494"
     CDS             5411..7879
                     /gene="gyrA"
                     /locus_tag="VYR08_001494"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV3467005.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            7879..8622
                     /gene="srtA"
                     /locus_tag="VYR08_001495"
     CDS             7879..8622
                     /gene="srtA"
                     /locus_tag="VYR08_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV3467006.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         2800 a         1490 c         1847 g         2527 t
ORIGIN      
        1 cgatatatta ataaaaaata tttagattta ccggaaacat tcgaaaatta taatgttttt
       61 gtaccagaag ctaatggaag tggtgcctta ggtgaagtct tatcaacacc cctaatcggg
      121 gaacccctaa tcgggcatac agatactttt ttatctattg gtaattttaa aacaaaattt
      181 gaagccgatg cttgtattaa atttattaaa actaaattcg ctagagtatt attaggtgtt
      241 ttgaaagtta ctcagcataa ttcacgcaaa acttggtatt acgtccccct ccaagacttt
      301 acggtcaatt cggacattga ttggacacaa tcagtgactg atattgaccg ccagcttgat
      361 caaaaatatg acctttcccc tgaagaaatt gcctttattg agaatcatgt aagggagatg
      421 gattagaaaa gtatttttat ttgacaaata gtgctcaatg atctaaaatg actatatagg
      481 attaggtcag gaagcatacg atgccctgac cctttttgta cttatgagat gagaaagtca
      541 tttgttagat aaattgactt cgttagcaaa cgttcaaaaa aggaaaactt atgccagtag
      601 aaattaaaac cactaaagaa attcatccta aaatctatgc ctacaccaca ccgacagtaa
      661 ccagtaatga aggctggatt aagattgggt atacagaacg tgatgtcaca caacgtatca
      721 aggagcaaac gcatacagct catatagcta cagatgtctt atggactggt gatgcagctt
      781 atacagaaga gcctgataag gggaaaactt tcaaggacca tgatttccac catttccttt
      841 ctttccatga tgtagaacgt cgtcccaaga cggaatggtt ctattttaat ggaactcctg
      901 aaaaatcaaa aaatcttttt gataagtttg ttcagcatga tttgtctggt tatcagcctg
      961 gaaaaggaca ggactatact ctgcgacaag agcaagaaga agcagttgct aagacattag
     1021 cttatttcca agaacatgct ggaggcaagt ttctctggaa tgccaagcca cgctttggta
     1081 aaaccttgtc tacctatgac ctagctcgac ggatggaagc tgtcaatgtc ctaattgtaa
     1141 caaaccgccc tgccattgct aactcatggt atgatgattt tgaaacattc atagcaggtc
     1201 aaacgactta caagtttgtt tctgaatcag atagccttaa gagtcgtcca atcttgtcac
     1261 gacaagaatt tcttggtatt ttagctgacg atgtaagaca acttgctttt atcagtctcc
     1321 aagacttgaa aggatctgtt tatttaggtg gagagcacga taaactcaaa tgggtaactg
     1381 atctgcattg ggacttgttg gttattgacg aggctcatga aggagttgat accttcaaga
     1441 ctgaccaagc ctttaataag attcgacgaa attttactct gcatttgtca ggtacatcat
     1501 ttaaagcatt ggctaaagga gattttacag aggaacaaat ctacaactgg tcttatgctg
     1561 atgagcaggc tgctaagtat tcgtggtctc ttgagcaaga agaggaaaat ccttatgaaa
     1621 gcttgcctca gttgaatctc tttacctatc aaatgtctca gatgattggc gaaaagttag
     1681 aaaaaggcgc tcagatcgat ggtgaaaata ttgactatgt ttttgactta agtgaatttt
     1741 tcgctacaga tgataaaggg aaatttattc atgagcatga tgtcagaaat tggttagata
     1801 ctctatcaag caatgaaaaa tatccatttt caaccaaaga actccgtaat gaactcaagc
     1861 atactttttg gcttttagaa cgtgtcgctt cggccaaagc attaaaagcc ctactagaag
     1921 aacacccaat ctatgaaaac tatgagatcg ttctagctgc tggtgacgga cgtatgtccg
     1981 aagaagacga taaagtcaaa ctcaaatcct tggacttggt tagaaaagcg atagcagaga
     2041 atgacaaaac cattacccta tccgttggtc agctgacgac aggtgtcact atccctgaat
     2101 ggacaggtgt attgatgtta tcaaatttga aatcaccagc tctttatatg caggccgcct
     2161 tccgtgctca aaatccttac tcatggagcg ataacaaagg aaatcacttt cgcaaagaaa
     2221 gagcctatgt atttgacttt gcgccggaaa gaaccttgat tctctttgat gagtttgcca
     2281 acaacttatc gcttgtaact gcagctggta gaggaacttc agctacacgc gaagaaaata
     2341 ttagagaatt attaaacttc tttccaatta ttgccgaaga ccgtgctggt aagatggttg
     2401 aaattgatgc aaaggcagtt ctaaccactc ctcgccagat aaaagctaga gaagttctta
     2461 aacgaggttt tatgtccaat ctcttatttg ataatattag tggtattttc caagcaagtc
     2521 aaacagtttt agatatttta aatgagctgc cagttgaaaa ggaagggaag gtacaagata
     2581 gttctgattt attagatttt tcagatgtta cagtcgatga tgagggaaat gcagtagtag
     2641 accatgaaat tgtagttaat cagcaaatgc gactttttgg tgaaaaagtt tatggacttg
     2701 gtgaatctgt tgctgagtta gtcacaaaag atgaggaacg aactcaaaaa cagctggtca
     2761 atgacttgag taagaccgtt tcttcagtga ttgtagagga attgaaagca gattattctc
     2821 taaaaacaag ggaaactgag caaattaaga aacaaattac agcaacactt gagaatgaaa
     2881 ttcgaaaaaa tgatatcgaa agaaaaattt ctgaagctca tatcaagcaa gagttgcaac
     2941 agcagctcaa agaagcaaat gataaagcgc aaaaagataa gattcaagaa gatttggaaa
     3001 aacgtttaga agaaaataaa ctcattcata aagaaaaact agaacaaaca ctcaaaaaag
     3061 aagtggaaaa aatgcctgag aaatttatcg aacaggttga gataaaacgt gtggaacagt
     3121 tgaaacaatc agctcaagat gaaattcgtg accatttacg agggtttgca agaacaattc
     3181 caagttttat tatggcttac ggtgatcaaa ctctaacact tgataatttt gatgcctttg
     3241 ttcctgaaca tgttttttat gaagtaacag ggattacgat tgatcagttt agatatttgc
     3301 gagatggtgg gcaggatttt gcagggcatc tctttgataa agcaacattt gacgaagcta
     3361 ttcaagaatt tcttcgcaag aaaaaggagt tggcggatta ttttaaagat caaaaagaag
     3421 acatttttga ctatattcca ccgcagaaga ccaaccaaat tttcactcct aaacgagtgg
     3481 tgaaaaggat ggtagatgat ttggaaaagg aaaatccagg gatttttgat gatccatcta
     3541 agacttttat tgatttatat atgaagtcag gcctctatat tgcagaactt gtgaagcggt
     3601 tatataatag caatggcttg aaagaggcct ttccaaatcc tgaagaacgc ttaaaacata
     3661 ttttggaaaa gcaagtttat ggatttgctc cgtctgagat tatctataac atttccacta
     3721 attttatatt tggtaatctt tctaaagata tcagtaggaa gaattttgtt ttagcagata
     3781 ccattccagc ggctaaagaa gggaacattc aaaagttggt tgattcctat tttgaaaata
     3841 attaaaaaga aggccgagtc aaaattcttt gaaatcagaa aaaacgcata atattgagtg
     3901 cttttgtact gccccccaaa agttagacag aaaaaatcta acttttgggg ggcagttcag
     3961 acaatccttg gtattatgcg ttttattgtg ggaagatgta taatggattg aaataagata
     4021 tgaacaaatc gattaggaaa ggaaaattga tttatagaaa tgttttagta gtcgatgcgt
     4081 actgttatag attcaacgaa ctataaaatc tgaagaaagc aattttaacc agatcttaga
     4141 agcagttgtg tactactcta acttcaatag actatatcat atagataaaa aacaactccc
     4201 tgatgattca aggagttgtc tatagttaaa ttagttttta gaagcttctt ggaattctgg
     4261 atttttccat gcttcgtcaa taatagcttg caattcttta gcagatgctt gcattttttg
     4321 agtttctgca tcgttcaatg gaatgtttac tggacgaacg ataccatgtg caccaacaac
     4381 agctggttga ccgataaaga cattctcaac tccgtattga ccttcttgga atactgaaag
     4441 tggaagtact gcgttttcat cgtcaaggat tgctttagtg atacgagcaa gggctactgc
     4501 gataccgtag tatgttgcac cttttttgtt gatgattgtg taggctgcat cacgaacacc
     4561 ttcgaacaat tcaatcaatt cagcttcttg aacattttga gtgtctttaa ggaattcttc
     4621 aaggtttaca ccagcgatgt tagcgtgtga ccaaacagcg aactcagagt caccgtgttc
     4681 acccatgatg taggcgtgca ctgaacgagc atccacatcc aatttttcag caagtgcttg
     4741 acggaaacga gctgagtcaa gtgaagtacc tgaaccgata acgcgttctt tagggaaacc
     4801 agagaatttc caagttgagt aagtcaaaac gtcaactgga ttagcagcaa caaggaagat
     4861 acctttgaaa ccagattcaa caacttgagt tacgattgat ttgttgatag caaggttttt
     4921 acctacaagg tcaagacgag tttcacctgg tttttgaggt gcacctgcag tgatcacaac
     4981 aaggtcagcg tctgcacagt cagagtattg agctgcatag atttttttag gtgaagtgaa
     5041 ggcaagggcg tgactaaggt caagcgcatc accaacagct ttttcatgca attgtggaat
     5101 ttcgataatt ccaagctctt gtgcaattcc ttggttaaca agtgcaaaag cgtaagatga
     5161 acctacagca ccatcaccga caaggataac ttttttgtgt tgtttagttg aagtcattgt
     5221 tttaaacatc tccttaattt tattagggga ttttccctag acaacttcat tctatcactt
     5281 ttaaaaaact ttgtcacgaa tatgccttat agttctcgat gtaaacgttt tagtggttta
     5341 gaggctgaaa tagatgggaa tttatggtat aatgttgtta cttactaatt gtgaaatgag
     5401 gcatttatta atgcaggata aaaatttagt gaatgtcaat ctgacaaagg agatgaaggc
     5461 aagttttatc gactacgcca tgagtgttat cgtagcgcga gctcttcctg atgttcgaga
     5521 tggcttaaaa cctgttcacc gtcgcattct ctacggaatg aatgaattgg gtgtgacccc
     5581 agacaaaccc cataaaaaat ctgctcgtat tacaggggat gtcatgggta aatatcaccc
     5641 acacggggat tcctctattt atgaagccat ggtccgtatg gctcaatggt ggagctaccg
     5701 ttacatgctt gtagatggtc atgggaattt tggttccatg gatggagata gtgctgccgc
     5761 tcaacgttat accgaggcac gtatgagcaa gattgctctg gaaatgcttc gtgatatcaa
     5821 caaaaataca gttgatttcg ttgataacta tgatgccaat gaacgggaac ccttggtctt
     5881 gccagcgcgt tttccaaacc ttttggttaa tggagcaact ggtatcgcgg ttgggatggc
     5941 aaccaatatt ccacctcata atctgggtga aaccattgat gcagtgaagt tggtcatgga
     6001 taatcctgaa gtgactacca aggacttgat ggaagtcttg cctggaccag attttccaac
     6061 tggtgctctt gtcatgggga aatcaggtat ccataaggct tatgaaacag gtaaaggttc
     6121 gattgtccta cgttctcgta cagagattga aacgactaag actggtcgtg agcgcatcgt
     6181 tgtaacagaa tttccttaca tggtcaataa aaccaaggtg catgagcata ttgttcgctt
     6241 ggttcaggaa aaacgcattg agggtatcac agcagtacgt gatgagtcaa accgtgaagg
     6301 tgttcgattt gttattgaag tcaagcgcga cgcctcagcc aatgttattc tcaataacct
     6361 cttcaaaatg acccaaatgc aaaccaattt tggtttcaat atgctcgcta tccaaaatgg
     6421 tataccgaaa attttgtctc ttcgtcagat tttggatgct tatatcgagc accaaaaaga
     6481 agtggttgtt cgtcgtacac gttttgataa ggaaaaagcg gaagcgcgcg ctcatatctt
     6541 agaaggtctc ttgattgcgc tagaccatat cgacgaagtg attcgtatca tccgtgctag
     6601 tgaaacggat gcggaagctc aagctgagtt gatgagcaag tttaagcttt ctgaacgtca
     6661 aagtcaagct atccttgata tgcgtcttcg tcgtttgaca ggtttggaac gcgataagat
     6721 tcaatctgag tatgatgacc tcttggctct gattgcggat ttagcagata ttcttgctaa
     6781 gcctgaacgt gtttctcaaa ttatcaaaga cgaattggat gaagttaaac gtaaattttc
     6841 tgataagcgc cgtacagagt tgatgattgg acaggtcttg agtctcgagg atgaggactt
     6901 gattgaagaa tcggatgtct tgattaccct ttctaacaga ggctacatta agcgtttgga
     6961 tcaggacgag ttcactgctc aaaaacgtgg gggtcgtggt gtccaaggaa cgggagtgaa
     7021 agatgatgac tttgttcgtg agttagtgtc aactagcacc catgatcatc tgctcttctt
     7081 cacaaacaag ggacgtgtct atcgtcttaa aggttatgaa attcctgagt atggtcggac
     7141 tgccaaaggg ctaccagtag tcaatctctt gaaattggat gaagacgaaa gtattcagac
     7201 ggttatcaat gttgagtctg atcgcagtga tgatgcttat ctcttcttta caacccgtca
     7261 cggtattgtg aagagaacca gtgttaagga gtttgccaat attcgtcaaa atggtctcaa
     7321 agcgctgaat ttaaaggatg aagatgagtt aatcaatgtc ttgttggcag aaggagatat
     7381 ggatattatc attggtacca agtttggtta tgcagttcgc tttaatcaat cagccgttcg
     7441 tggtatgagt cgtatcgcca ctggtgtgaa aggtgttaac cttcgtgaag gagacacagt
     7501 tgttggtgcc agcttgatta ctgatcaaga tgaggttctt attatcacag aaaaaggata
     7561 tggtaagcgt acagtcgcta ctgaataccc aacaaaaggt cgtggtggta agggaatgca
     7621 gacagctaaa attaccgaaa aaaatggctt gctggccggt cttatgactg ttcaagggga
     7681 tgaggatttg atgattatca ctgatacagg tgtcatgatt cgaaccaatc ttgccaatat
     7741 ttcacaaaca ggacgtgcaa ctatgggagt taaagtaatg cgcctggatc aagatgctca
     7801 gatagtgact ttcacaacgg ttgcggtggc agaaaaagaa gaagttggga cagaaaacga
     7861 aacagaaggt gaagcataat gtctcaaaaa aataataaaa agaaaaacaa gcgaaaaaat
     7921 ctgctgacaa atatcctagc aggatttctg atattactgt cactggcttt gatttttaat
     7981 actcaaattc gaaatatttt catagtctgg aataccaata agtatcaagt tagccaggta
     8041 tcaaaagaaa aattagaaga aaatcaggat acagaaggca attttgactt tgattctgtc
     8101 aaagctatct cttcggaagc tgttctaact tctcaatggg atgctcaaaa attaccagtt
     8161 attgggggaa ttgcaattcc tgaattggaa atgaatttgc cgatttttaa aggacttgat
     8221 aatgttaatc tcttctacgg agctggtaca atgaaacgcg agcaagtaat gggagaagga
     8281 aattatagtc tagctagtca ccatatcttt ggtgttgata atgctaataa aatgttattt
     8341 tctcctttag ataatgctaa aaatggcatg aagatttatc taaccgataa aaataaagtt
     8401 tatacttatg aaatacgtga agtcaaacgt gtgacaccgg atcgtgttga tgaagttgat
     8461 gatagagatg gggtcaatga aatcacatta gtaacctgtg aagaccttgc tgctacagaa
     8521 cgtattattg tcaaaggtga tttgaaagaa acaaaagatt attcacaaac atctaatgaa
     8581 atcctaacag ctttcaatca accatataaa caattttatt aatacaaatc agtgaaatcc
     8641 atgatttcac tgatttttta agat
//
LOCUS       DAVMHG010000087         8637 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMHG010000087 DAVMHG010000000
VERSION     DAVMHG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.087"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..659)
                     /locus_tag="VYR08_001496"
     CDS             complement(<1..659)
                     /locus_tag="VYR08_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3467007.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     L"
     gene            complement(715..1737)
                     /locus_tag="VYR08_001497"
     CDS             complement(715..1737)
                     /locus_tag="VYR08_001497"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV3467008.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(1869..4340)
                     /gene="parC"
                     /locus_tag="VYR08_001498"
     CDS             complement(1869..4340)
                     /gene="parC"
                     /locus_tag="VYR08_001498"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV3467009.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDVAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(4760..6703)
                     /gene="parE"
                     /locus_tag="VYR08_001499"
     CDS             complement(4760..6703)
                     /gene="parE"
                     /locus_tag="VYR08_001499"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV3467010.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLADKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            6839..7480
                     /gene="plsY"
                     /locus_tag="VYR08_001500"
     CDS             6839..7480
                     /gene="plsY"
                     /locus_tag="VYR08_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV3467011.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(7523..8637)
                     /locus_tag="VYR08_001501"
                     /pseudo
     CDS             complement(7523..8637)
                     /locus_tag="VYR08_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2327 a         1944 c         1625 g         2741 t
ORIGIN      
        1 tccaattttt ctttttgcca ttttttaaaa acctcgttat attcttttgt tgtaacaata
       61 tctttttgca atttcattcc tttaaagata tatggttccc ccttaatacc aacttgtgag
      121 tatggttttg agaatggtac tacgttactt acaactggag aaccaccaga tgaagctgtt
      181 ggcatcaata atgaactatc tgtcgaccaa gcttgagctt tggcatattt ttcatatctc
      241 ttctctaggt cagtggtctc agaaacagca tcttctaaca atttcttata tttatccaaa
      301 ccaggtttag ctacaacatc cttatctttt cctttcgtaa tgccaaggtg tttcatggca
      361 gaaccagatt ttggatccat aatattcaag taagacgctg gatcttgata gcttggagcc
      421 catcctgtac tgttcaaatc atagtctttt tgagaaggag caacattgcc gtatttatca
      481 ttttccatca aaccatcaat aacatttcca ataacgtctg tcctcgatgt tcgagtcgct
      541 atactgtagc ccaatgatgc tggatctact gcatagacat aagaaaatgt tgtcggtgca
      601 tctgcttctt tatcagtttt tccacaagcc actaaaatag ctgacgtgct caggaccact
      661 cctgctgtta agagccactt tttctatttc ataaaaaatc tcctttggtt tattttaatc
      721 tacttttaca atccaacctt ctggcgcttc aatatcgcca aactgaatac ccgtcaattc
      781 attatataat ttacgcgtca caggacctac ttctgtttca ctatagaata catggaaatc
      841 atcaccatgt tgaatacctc caattggaga aataaccgct gctgtaccac aggcacctgc
      901 ctctacaaaa cggtcaagat tatcaattgg aacatcaccc tcaataggag ttaatcccaa
      961 gcgatgttct gccaaataaa gcaaggaata cttggtaata gatggcaaga tagatggact
     1021 caatggtgtt acaaattcat tatcagctgt aattccaaag aagttagctg atccgacttc
     1081 ttcaatcttt gtatgagttg atgggtccag atagataaca tctgagaaat gacgtgactt
     1141 ggccattttt cctggtaaga gacttgcagc atagtttcca ccaaccttag ccgcacctgt
     1201 accatttggt gctgcacggt cgtactcatc ctgaatcaag aagttggttg ggaccaaacc
     1261 acctttaaag taatttccaa ctggcatagc aaagatggtg aaaatgtact cttctgccgg
     1321 ttttaccccg ataatatctc cgacaccaat caaaagaggg cgaagatata aggttccacc
     1381 tgttccgtat ggtggtacgt attcttcatt cgcacggaca actgctttac aagcttctac
     1441 aaacatgtct gtcggaactt gtggcatcaa gagacggtca catgtacgtt gcagacgttt
     1501 agcattttca tcaggacgga acagttgaac actgccatcc ttagtacgat aagctttcaa
     1561 accttcaaat gcttgttgtc catagtgaag acttggagaa gactctgaaa tatgcaaagt
     1621 tgcatcctct gtaagctctc cttgatccca ttgtccattt ttgaaatgag caagatagcg
     1681 ataaggtaat ttcatatagg aaaaaccgag gttttcccaa tcaatcgtta ctgtcatatt
     1741 ccactcctta ttctaaaaac ctatttctta tattctacac tatttttcta aaatagcaag
     1801 tatattttgt aattttcaga aaatttctcc aataaaaacc agctcttaga actgattctt
     1861 catttcactt atttatcttc agtaactact tcctgaagat aagcgtcaaa aacttcttca
     1921 tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc gttctgacaa gttcaagtct
     1981 tgcaatcggc tttcatagat tgttccttta ttggattgga caagcagagt ttggtcgttc
     2041 acatccactt ccgtactgaa gaaatcgcca acaaatcctt gctctgcaac tgctcctgcc
     2101 aagaagacac gatgcggttt gtttttcaac tcacgcaaga cttgtaatcc tcgtttggca
     2161 cggctggttg ctagaatttc ctcaatggaa acacgtttca agcttccacg ctgggtcaag
     2221 aggtagaagg acgaagtatt acagataaag ccagattgga ggacatcatc ttctttcaaa
     2281 ttcatagcct tgacacctgc tgccttagca ccgacaaccg gaacctcttc gatattgaaa
     2341 cgcagggcat aaccattttg actaaccaag acaacatcat ctagtttaat cggagccact
     2401 gctacaatct gatctgtatc gtctttgagc ttagcatact tgacagactt agatctatag
     2461 gtccgccatg gagtgaattc ttttcgctct acccgtttga tttgaccaag gcgagtcact
     2521 gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt ccacataaag gatttcttca
     2581 ttcgtttcaa agtttgtgat ggtttggctc agatgctctc cgatgtcctt ccaacgaata
     2641 tctgccaact catggattgg tctgtagatg acatttccaa gacttgtgaa catcaagagg
     2701 tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt catcatcacg cttgccaatt
     2761 tcttccaagg tggaagccgc aaaggaacgt ggactggtac gcttgatgta acctgccttg
     2821 gtcacgctga cgtaggtatc ttcctcagcg ataagactag ctgtatcaat ctcaattgct
     2881 ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa atttcttctt gacctcacga
     2941 agttctttct tcatgagatt gtacatagtc ctttcatcac cgataatagc cgccagcata
     3001 gcaatcttct cacgaagctc tgcttcttct tcctgcaaga caaccacatc ggtattggtc
     3061 aaacggtaca gttgcaaagt tacgatagcc tcagcttgtt cttccgtaaa atcatagcta
     3121 actttgaggt tttccttggc gtccgcctta ttctcagaag cacggataag agcaatgact
     3181 tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga tatggagacg tttctcagcc
     3241 ttttctttgt caaagcgtga acgcgccaaa atcacttctc gacggtgagc gatgtagcta
     3301 gacaggattg gaacaatccc aacctgacga ggtgtgaaat tgtcaatcgc caccatatta
     3361 aagttgtagt tgatttgtag gtcggtgtac ttaaataagt agttgagaac aagctcagta
     3421 ttagcgtctt tcttaagttc aatagcgata cgaagaccat cacggtcaga ctcatcacga
     3481 acctcagcaa tcccagctac cttgttatta acacgaacat catcgatttt cttgactaga
     3541 ttggccttat tgatttcata aggaatctca gtaataacga tttgttcctt accacctttt
     3601 agcttttcaa tttcagtctt ggaacgaaca accacgcgcc ctttcccagt ctcataagct
     3661 ttcttgattt catcacgacc ctgaataata gcccctgtag ggaagtctgg tccaggtaag
     3721 aattccatga gtttatcaat ctttgcagtt gggtggtcaa tcatgtaaac tgcaacatct
     3781 atgacctcag ctaaattatg gggaggaatg tctgtggcat aaccagccga aatcccagtc
     3841 gaaccattga ccaagaggtt tggaaaggct gctggcaaaa ccgttggttc tttctccgta
     3901 tcgtcaaagt tccatgcaaa aggaactgtc tttttctcga tatcctgaag aaggtagcct
     3961 gcaatttcag acaaacgtgc ctcagtataa cgcatagccg caggaggatc tccgtccata
     4021 gaaccgttat taccgtgcat ttcaactaga atctcacgat ttttccagtt ctgtgacata
     4081 cgaaccatgg catcatagat agaagaatcc ccgtgtgggt ggaaattccc catgatgttc
     4141 ccgactgact tggccgactt acggtagctc ttgtcaaaag tattgctatc cttattcata
     4201 gaataaagaa tacggcgctg aaccggcttc aacccatcac gaatatctgg caaagcccgg
     4261 tcttgaataa tgtacttgga gtagcgacca aagcgctctc ccatgatgtc ctccagggac
     4321 atgttttgaa tgttagacat aagatacaaa gcccataaaa taccaagtga aaatagaaaa
     4381 ttcttgaagt aagcaaactc acaagagaat ttatcttttt cacacagtat ctagggcgtg
     4441 ttcaactcct ttcaaagaat gtagagtagg tttttatgca gtaaaagata ttttacggga
     4501 attcgtcccg tgttcagtta cgataagtaa ccaaactatc ctgtttgtat ttttcaatat
     4561 gaaaatatgg ttttccaaaa ttagtcttag tttgtgtctt agccgctccc ttaaacgcct
     4621 ctttgagata agcactcata gcagattctt cattaataat cctgcaattt tttcaaacca
     4681 agattttcaa actgcttttt cacatagtca ttcacatccg actctaattt ccagtttact
     4741 aacatattat tttctttcat taaaacactg tcgcttcttc tagcgtaaac ttgacattat
     4801 cttcaatcca tttacggcgt ggttctacct tatctcccat gagaacattg acgcggcgtt
     4861 cggcgcgcgc taaatcttca attgtgacac ggatgagggt acgtgtttct gggttcatgg
     4921 ttgtttccca gagctggtcc gcattcatct caccaagtcc tttgtatcgt tggagggtag
     4981 cgcctttacc gaactgttta cggagttctt ctagttctcc gtccgtccaa gcgtaggcca
     5041 cttcttcttt cttgccttta cctttggaca tcttgtaaag aggtgggagg gcaatataga
     5101 catgacctgc ctcgactagc ggacgcatgt aacggtagaa aaatgtcaag agcaaggtct
     5161 ggatatgggc accgtcggta tccgcatcgg tcatgataat gatcttatca tagttggcat
     5221 cttcaataga gaagtctgct ccaacacccg caccaatggt ataaatcatg gtattgatct
     5281 cttcattttt gaggatatcc gccatcttgg ccttggctgt attgacaacc ttaccacgaa
     5341 gaggtagaat agcctggaac ttgcggtcac gaccttgttt ggcagaacca ccggcagagt
     5401 ccccctcaac tagatagagt tcattcttag caggattctt agattgggct ggggtcaatt
     5461 tcccagacaa caagccctta tctttcttgt ttttcttccc atttcggctc tcatcacgcg
     5521 ccttacgtgc tgcttcacga gcatcacggg ccttgatagc cttgcggatg aggttagaag
     5581 ctaattcccc attttccata aggaaaaagg tcaacttatc agccactatt ccatccacaa
     5641 ctgggcgagc tagggggctt cctagtttat ccttggtctg tccttcaaac tgcaagtgtt
     5701 cttcaggaac taagatagaa agaacggccg ctagtccctc acgatagtct gaaccttcaa
     5761 ggtttttatc tttttccttg agaagacctg ttttacgtgc atagtcattc atgaccttgg
     5821 taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc cttggtgcga acgttattga
     5881 caaaggatag aatgttatct gagaatccgt cattgtactg gagggctact tccacttgaa
     5941 aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa gatttcctta tcttcgttga
     6001 gataagaaac aaaatcttgt actccattct catagtggaa ctcaatcgct tcatctgttc
     6061 gcttgtccgc taaagacaag gtcacatttt tcaagagaaa ggctgattca ttaaggcgct
     6121 ctgaaatggt attgtacttg aaatctgtcg tagaaaagat agtcgcgtca ggcataaaag
     6181 taactttggt gcctgtttta gacttgagtg ctgtaccgat tttcttcaaa gtcgtgacag
     6241 gttttccacc attttcaaaa cgttgcttgt aaactgcgcc atcacgggta atttcaactt
     6301 ctaaccagct agaaagggcg ttaacaacgg aagaacccac tccgtgaagt ccacctgatg
     6361 tcttatagcc accttgaccg aatttccctc cggcatgaag aatggtaaag ataacctcaa
     6421 cagttggaat tcccatagcg tgcatacctg tcggcatccc acgtccatgg tcttgaaccg
     6481 ttagactacc gtctttattg atagttacat caatacgatc accaaaccca gacaaggctt
     6541 catcgactgc attatcaacg atttcccaaa ctaggtgatg aagaccagcg ccatcggtcg
     6601 atccaatata catccctgga cgttttcgga ccgcatccaa cccttctagc acctgaatag
     6661 catcatcatt ataattgtta atattgattt ccttttttga cacaaggaac ctcctattcg
     6721 ttcatcttta ctattctaca ggttttccaa ggattttgca aaatttttct ttctccgatg
     6781 tgacaatttc agcagagatt ctctgctttt ctttcccaat tcatgatata ataggagtat
     6841 gattacaata gttttattaa tcctagccta tctgctgggt tcgattccat ctggtctctg
     6901 gattggacaa gtattctttc aaatcaatct acgcgagcat ggttctggta acactggaac
     6961 gaccaacacc ttccgcattt taggtaagaa agctggtatg gcaacctttg tgattgactt
     7021 tttcaaagga accctagcaa cgctgcttcc gattattttt catctacaag gcgtttctcc
     7081 tctcatcttt ggacttttgg ctgttatcgg ccataccttc cctatctttg caggatttaa
     7141 aggtggtaag gctgtcgcaa ccagtgctgg agtgattttc ggatttgcgc ctatcttctg
     7201 tctctacctt gcgattatct tctttggagc tctctatctt ggcagtatga tttcactgtc
     7261 tagtgtcaca gcatcgatcg cggctgttat cggggttctg ctctttccac tttttggttt
     7321 tatcctgagt aactatgact ctctcttcat cgctattatc ttagcacttg ctagtttgat
     7381 tatcattcgt cataaggaca atatagctcg tatcaaaaat aaaactgaaa atttggtccc
     7441 ttggggattg aacctaaccc atcaagatcc taaaaaataa aacgccagtt ctgtactgcc
     7501 cccaaacagt tagacaaata atttatccaa aggatttagt tctgtattgc acagggctaa
     7561 gtccttttag ttttacctta attcgtttgt tgttgtagta atcaatatag tctataatgg
     7621 cttgttccaa ttggttaagt gatttaaatg ttttctcata gccataaaac atttcggatt
     7681 ttaaaatgcc aaagaaagat tccatcatac cgttgtcttg gctgtttccc ttgcgtgaca
     7741 tagatgcttg aattccctta ctctctagga accgatgata agaatcgtgt tgatattgcc
     7801 agccttggtc actatggaga atcgtattct cgtagtgctt ctctgtaaag gcctgttcta
     7861 acatactctt cacttgttct aagttgggcg aagttgaaag attataggcg ataatttcgc
     7921 tattaaagcc atctaaaact ggtgataagt aaagcttttg agtacttact ggaatggcaa
     7981 attcagtcac atctgtgtag cactgttcca ttgttttaga gccttcaaat tggccttgaa
     8041 tgagattctc tgctttcttg ccaacgtctc ctttatgaga agaatatttt cgtttctgtc
     8101 gcgttttagc ttgtaaattg agtacttcat caagtgttga actcttttat gatttaccag
     8161 ataaccacga tttcttagtt ctaaataaat ccgacgataa gcataatttc ccttgtgttc
     8221 gataaagatg gattgaattt cagctttaag ctcttggtcc ttatctgttt tgtctagctg
     8281 tttcaagtga tagtagtagg tcgaacgagc tagtttaatg gcttttagaa gaagatctaa
     8341 cgaaaactca gtcattaatt cttgaacaat ttctgtcttt cttctttctc tttttcctcc
     8401 ttcaatcgga gttctcttaa cttttttagg atggcattct ccgctctcag gtactcattt
     8461 tctgcttgaa gacgttctaa ttctgtcctc tcttcaggtc tcgtttttgg attacgtccc
     8521 attttaggta ctctccctct tggtttctca acaatagtat acccgttttt cctgtattgt
     8581 gctagccagt taagaagtat cgtacgactt gggagaccgt attcaagaga aactcta
//
LOCUS       DAVMHG010000088         8550 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMHG010000088 DAVMHG010000000
VERSION     DAVMHG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.088"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1473)
                     /locus_tag="VYR08_001502"
     CDS             complement(<1..1473)
                     /locus_tag="VYR08_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3467012.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QAAQKTENLTPDEVSKKEGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEERIARIIPLRYRSNHWVPDSRPEEPSPQPAPNPQPAPSNPIDEKLVKE
                     AVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTGLPSSD
                     REFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDV"
     gene            complement(1727..3460)
                     /gene="ptsP"
                     /locus_tag="VYR08_001503"
     CDS             complement(1727..3460)
                     /gene="ptsP"
                     /locus_tag="VYR08_001503"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV3467013.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(3466..3729)
                     /locus_tag="VYR08_001504"
     CDS             complement(3466..3729)
                     /locus_tag="VYR08_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV3467014.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4080..4298
                     /gene="nrdH"
                     /locus_tag="VYR08_001505"
     CDS             4080..4298
                     /gene="nrdH"
                     /locus_tag="VYR08_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV3467015.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            4379..6538
                     /gene="nrdE"
                     /locus_tag="VYR08_001506"
     CDS             4379..6538
                     /gene="nrdE"
                     /locus_tag="VYR08_001506"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV3467016.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            6726..7688
                     /gene="nrdF"
                     /locus_tag="VYR08_001507"
     CDS             6726..7688
                     /gene="nrdF"
                     /locus_tag="VYR08_001507"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV3467017.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            7975..>8550
                     /locus_tag="VYR08_001508"
     CDS             7975..>8550
                     /locus_tag="VYR08_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV3467018.1"
                     /translation="MLKQEKLTKILETVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIARFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGE"
BASE COUNT         2489 a         1792 c         1627 g         2642 t
ORIGIN      
        1 gacatccttg agtcgttcca acaggttatc caaagcctca aaatcaactt gtcgaccttt
       61 attatcaagt aaatcttggt gaattcttgc tagtaagtca taagccttat tgtaaaattc
      121 tcgatcacta gatgggaggc cagttttctt agctcctagc ttatgagata aactttcctg
      181 cttggccagt ttgctatcaa tgcctgctgc tgtttctgct gaaagatcct tggctgggat
      241 ataacgagaa actccattct cctcaaagac ataaccatcg cctacttttc gaacagcttc
      301 tttgaccaat ttctcatcaa ttggattgct tggagctggt tgaggatttg gtgcaggttg
      361 tggacttggt tcttctggtc ttgaatctgg tacccaatgg tttgaacgat aacgaagggg
      421 aataatacga gcaattcgtt cttccaattc agacatttgt tcataaggga taaagtggta
      481 atggttacca tgagggacag ctacacctct ggcggttcga cttgtgattt gcgctgggtc
      541 gaaaataagg ccatcagatt ctacatggcg ttgactcaaa ggcagtttgt agagctgttt
      601 caagagacta tcaatgtcat tactttgact tgcttgactg ttagtgttgc tgttgttgct
      661 tgtgttagta tttgtagttc ctggattgct tacagaaggt acccagtttg ttcttggagt
      721 gttatcgcta ttttgtcggc gataggttct taaatttgac agattttccc gaccagatag
      781 gaaggcttct gcagcagcca actcgctagc tgataactca ttcttaggaa tgtaatggta
      841 atggtcgccg tgaggaacga tataagcatc acccgtgtcc tcaatgatat cagatgcatt
      901 gaagatatac ccatcatctg tggtgtagcg tccctgtgaa cgtgcaaagg ctaccgcacc
      961 atcgtttgct gaagtccctc cttcacgatg ctgactatgt tcttgttttt gccgattgat
     1021 ttcttctttt gtacggacat tatccgcgtg ggcagcatcc ttaaggtaaa catagtattt
     1081 tccatctacc ttgataacat aaccaccctt gatttcactg ataatatcct cgtcttttag
     1141 ctggtagttt ggatccttca tcagcaattc ttcactgatg atagcgtcat aaggaacctt
     1201 cccattgtaa taatgatagt ggtccccatg tgaagtcaca tagccttggt ctgttatctt
     1261 gatgacgatt tgttcggcat tgataccttc ttttttactg acctcatcag gagtcagatt
     1321 ctccgttttt tgtgctgctt gtttaccatc tatataggaa acacgattat tttccttaac
     1381 cgttctagct tgatacagtc ccaactcgta agaacaaaca cttaaaatca aagctgccgc
     1441 agaaccaaca aggtatttct tattaatttt catcgaaact ctctttctat ttgatatttc
     1501 tagttgcaat tcatttctaa attgcagaaa ctcctctcta ttacttaact ggttaatagt
     1561 ttactcctaa atctccaact tgtcaagaat tttatgaacc atttaagtat tttttgttct
     1621 gaccactact ttttcaaggt tggactttct ataagaaaga ttttcttgaa aattcttttt
     1681 aaaatatcaa aaaaatccct gtaactgagt tgcagggact tttcgattaa tcaaaattaa
     1741 cgtattcttt ttgaagttca agaacttctt ccattgttga gcattctgta agggcacggt
     1801 ttgcgtactc ttccatctta gctgtgtcga gtttcttcat caagctgcgt gtacgaagta
     1861 cagatgttgc tgacatagag aactcatcca agcccattcc gacaagaaga ggaacagctt
     1921 gttggtcacc agccatctca ccacacatac cagcccattt accttcagcg tgagctgctt
     1981 tgatcacatt gttaatcaag cgtaggattg atgggttgta tggttggtaa aggtatgaaa
     2041 cttgttcgtt catacggtct gctgccattg tatattggat caagtcgttt gtaccaattg
     2101 agaagaagtc aacttcttta gcaaattggt ctgcaagcat agccgctgca ggaatctcga
     2161 tcatgatacc aacttggatg ttatccgcaa ctgcaacacc ttcagcaaga aggtttgctt
     2221 tttcttcatc aaagactgct ttcgctgcac ggaattcttt caagagcgca accattggga
     2281 acatgatacg caattgaccg tgaacagacg cacgaagaag agcacggatt tgtgtgcgga
     2341 acatagcatc tccagtctca gagatagaga tacgaagagc acggaatcca aggaatgggt
     2401 tcatttcgtg aggcatatcg aagtaaggaa gttccttatc tccaccgata tccattgtac
     2461 gaacgacaac aggtttaccg ttcattcctt caagaacagc cttgtatgct tcatactgct
     2521 catcttcagt tgggaagtct tgagaatcca tgtacaagaa ctctgtacgg taaagtccaa
     2581 cagcttctgc accgttgttg ttaacacctt caacgtcttt tggagtacca atattggcag
     2641 ccaactcgaa gtgtttaccg tcagcagtca ctgtttgagc atctttcaaa agtgcccatt
     2701 cagctttttg tttcgcatag gcttcaccag ctgctttaaa ttctgccgct tgttcatctg
     2761 ttgggttgat aatcacttct ccagtgatcc cgttaacagc aaggatgtca ccgtctttaa
     2821 cgatttcagt gatgttgttt gtacctaata cagcagcaat ttcaagtgta cgtgccatga
     2881 tagctgagtg gcttgtacgt ccaccgatgt tggttacaaa agcttttaca aagtttttgt
     2941 ccaattgagc tgtatcagat ggtgtcaagt catgtgcaat cacaatcact tcttcattga
     3001 tagaagctgg gtttggcaat tttttaccaa gaaggtttgc caatacacgt tttgtcacgt
     3061 cgcggatatc cgctgcgcgt tcttgcatgt atgggttgtc ttccatgcct tcaaagatag
     3121 tgataaacat atctgtaact tctttcagac ctgcttctgc attcactttc ttcgcacgga
     3181 tagtttcctt gatttggctg atcatttctg ggtcagcaag aaccattaag tgagcatcaa
     3241 aaacttgagc tgcttcttca ccgagcgtac ctactgcttt ctcgcgaata acagaaagct
     3301 cgtcttgtga tgcctgtaga gcggcatcaa ggcgagcttc ttctgcgttt gtatcttcga
     3361 ctgtaatagt ctcaaatgac aaatccggct gaacgagtag atatgctttt gcaactgcaa
     3421 caccgtcaga tgctgcgatt cctttaagca tttctgtcat ttcccttatg ccaatccttc
     3481 tttttccatt gtttctgaga ttgcagcgat agcgtcatct gcatctgcac cttcagctga
     3541 gatagttacg tcagcacctt gaccaacacc aagactcata acacccataa ttgatttaag
     3601 gttaactgat ttacctttgt actcaagagt gatatctgaa gcaaatttgc tagcagtttg
     3661 taccaacaat gttgctggac gtgcgtgaat acctgtttct gccactacgt ggaaatcttt
     3721 agaagccata gtttgactct ccttttgttc tttctttttt tgagttatat gtgataaccc
     3781 ttacaattta gtattatacc atcttctagg atttttttca agcattttct ggattttcct
     3841 tcaatttcct ttcagataac ttgctgaaaa tcttctatcc tagataacaa aacacaggat
     3901 atagtttctt caaaaacaat ttataggata atcattgcta tttcacaata caaacactat
     3961 atattgtgtt tgtatatgaa aacaacaaaa gaacaccact atatttagtt taaatcattg
     4021 acaaaatttt attttctgat atactaagat agtattaaat tttgaagagg agttacacaa
     4081 tggtaaccgt ttattctaaa aacaattgtg tccaatgtaa aatgaccaag cgtttcttgg
     4141 acagtaataa tgtctcttat cgtgaaatca atcttgatga gcaacctgag tacgtcgatc
     4201 aagttaaaga gctcggtttt agcgcagctc ctgttatcca aacaccaact gaagtctttt
     4261 caggtttcca accaggaaaa ctgaaacaat tagcataatc ttagtacatc atccagaaga
     4321 aactgctttt agggctaact tagaagcctt tcttttgtaa ttagataagg gaaattttat
     4381 gggattaaaa catcttgaag acgtgactta cttccgtctt aataacgaaa ttaaccgtcc
     4441 tgttaatgga caaatcatgc ttcataaaga caaggaagcc ttggatgctt tctttaaaga
     4501 aaatgtagtt ccaaacacta tggtttttga ttcaatcaaa gacaaaatca attacctcat
     4561 tgaacacaac tacatcgaaa cagcctttat caagaaatac cgtccagagt tcttggaaga
     4621 attagctcaa tttatcaaag accaaaactt ccaattcaag tcattcatgg cagcttataa
     4681 attttacaat caatatgcct taaagactaa tgacggtgaa tactatcttg aaaatatgga
     4741 agaccgtgtc ttcttcaacg ccctttattt cgctgatggg aatgaagctg ttgcaatcga
     4801 tattgccaat gaaatcatcc accaacgcta ccaacctgct actccttcct tcttgaatgc
     4861 tggacgtgct cgccgtggag agttggtatc ttgtttccta atccaagtga cggatgatat
     4921 gaactctatc ggacgttcta tcaactcagc tcttcaactt tcacgtattg gtggtggtgt
     4981 gggaattacc ctcagcaacc ttcgtgaagc tggtgcacct atcaaaggct atgaaggagc
     5041 tgcttcaggt gtcgttcctg ttatgaagct ttttgaagac agcttctctt actccaacca
     5101 attggggcaa cgtcaaggtg ccggtgttgt ctacctcaac gtctttcacc cagatatcat
     5161 cgctttcctt tcaactaaga aagaaaacgc tgatgaaaaa gtacgtgtca agactctatc
     5221 acttggtgtt gtagtacctg ataaattcta cgaattggct cgtaaaaatg aagaaatgta
     5281 cctcttcagc ccttactctg tagaaaaaga atacggtgtg ccattcaatt acatcgacat
     5341 tactgaaaaa tacgatgaat tggtcgcaaa tccaaatatt cgcaagacaa aaatcaaggc
     5401 gcgggatttg gaaactgaaa tctctaaatt gcaacaagag tctggctatc cttatgtagt
     5461 caacatcgat acggctaacc gtgcaaatcc tgttgatgga aagattatca tgagtaactt
     5521 gtgttctgag attcttcaag ttcaagaacc aagcttgatc aacgatgctc aagaattcct
     5581 tcaaatggga acagatgttt catgtaacct gggttcaacc aacgtggtta acatgatgac
     5641 ttcacctgat tttggtcgtt ctatccgtgc tatggttcgt gcccttactt tcgttacaga
     5701 tagttcacat atcgtagctg taccaacgat tgaccatgga aatagccaag cccacacctt
     5761 tggtctcggt gccatgggac tgcacagcta ccttgcccaa caactcattg aatatggttc
     5821 acctgagtct gttgaattta caagcatcta ctttatgctt atgaactact ggaccttggt
     5881 agagtcaaac aatattgcgc gtgaacgtgg tattaccttc cacaactttg aaaaatcaga
     5941 ctatgctaac ggaagttact ttgacaagta tgtaactggt gaatttgttc caacatcaga
     6001 ccgtgttaaa gaactcttca aaaatgtctt tatccctggt gttgctgatt gggctgaact
     6061 tcgcgacaag gttcaagaag atggtcttta ccaccaaaat cgccttgctg tagcgccaaa
     6121 tggttctatc agctatatca acgacgtttc tgcttctatc cacccgatta cgcaacgtat
     6181 cgaagaacgc caagagaaga aaattggtaa aatctactac cctgctgctg gcttgtctac
     6241 agaaaccatt ccttactaca cttctgccta cgatatggat atgcgtaaag tcatcgatgt
     6301 ttacgctgct gcgactgaac acgtggatca aggactttca ctcaccctct tcatgcgtag
     6361 tgacattcca aaaggccttt acgaatggaa gagggaaaat aaacaaacga cacgtgactt
     6421 gtccatcctt cgtaactatg cctttaacaa gggaatcaag tctatctact acgtccgtac
     6481 ctttacagat gacggtggag aagtcggcgc caaccaatgt gaaagctgtg tgatttagtt
     6541 tctagcatag aaacattatt tttccctaag cagcactgat aaaatcaaat ctactatgtc
     6601 ataacataat ttatgtaaca ttttaaaagt cgggcttccg actttttgtg cgatactcct
     6661 atagaattat ggtaaagtag aaataattgt gagttcgcaa tactaaacac agaaagagat
     6721 tttaaatgga aacttactac aaagccatta actggaatgc catcgaagat gtcatcgaca
     6781 aatcaacttg ggaaaaactg acggagcaat tttggctcga tacacgtatt cccttatcaa
     6841 acgacttgga tgactggaga aagctatcta acaaagagaa agacttggtc ggaaaagttt
     6901 ttggtggttt aacccttctt gacactatgc aatctgaaac tggggttcaa gcccttcgcg
     6961 cagacatccg tacaccacat gaggaagctg ttttcaataa catccaattt atggaatctg
     7021 tccacgctaa atcttactca tcaatctttt ctaccttgaa tactaaggct gagattgaag
     7081 aaattttcga atggaccaat accaatcctt acctacaaaa gaaggctgag attgtcaacg
     7141 aaatctacct aaacggcagc ccacttgaaa agaaagttgc cagcgtcttc ctcgaaacct
     7201 tcctcttcta ctctggtttc ttcactcctc tctactatct cggtaacaac aaactagcca
     7261 acgttgcaga aatcattaaa ttgattattc gtgacgagtc tgttcacgga acctacattg
     7321 gttacaaatt ccaacttggt ttcaatgaat tgcctgaaga agagcaagaa aaactcaaag
     7381 aatggatgta cgacctactc tatactcttt atgaaaatga agaaggctac acagagagtc
     7441 tttatgacgg tgttggttgg actgaagagg ttaaaacctt ccttcgctac aatgccaata
     7501 aagctctcat gaacatggga caagatccac ttttcccaga ttcagctgaa gatgtcaacc
     7561 caatcgttat gaacggtatt tcaacaggaa cttccaacca tgacttcttc tctcaagtcg
     7621 gaaatggtta ccttcttggc gaagttgaag ccatgcaaga tgatgactac aactacggtt
     7681 tggactaata ctcttcgaaa atcaaattca aaccacgtca gcttcgcctt gccgtactca
     7741 agtacagcct gcggctagct tcctagtttg ctctttgatt ttcattgagt ataattctct
     7801 accactctta accatcaaaa agtaaacttg aaacaacaaa ataaaacaaa aacaaaaata
     7861 tcgaagtttt gaaaacaaac ctcgatattt ttgtttttgt tttattttgt tgtttttgta
     7921 tttgaatgat gagctagttt atggtaagat agtagtagga aacgaggtga taatatgctc
     7981 aaacaagaaa aattaaccaa aattttagag acagtaaata gtaaaggaac cataacggtt
     8041 aaacagatta tggacgaaat agccgtttca gatatgactg caaggcgcta tttacaggaa
     8101 ttagctgata aagatttgct gattcgtgtg catggtggag ctgaaaaact tcgaaccaac
     8161 tcccttttga ctaatgagcg atcaaatatt gaaaaacaag ccctccaaac ggcagaaaaa
     8221 caagaaatag cccgttttgc aggcagtcta gtagaagaaa gagaaactat ttttattgga
     8281 ccaggaacaa cattagaatt ttttgcacgt gagttgccta ttgacaatat ccgcgtcgtg
     8341 accaacagtc tacctgtttt tctgatttta agcgaacgaa aattaacaga tttgattttg
     8401 ataggtggaa attatcgcga tattacaggt gcttttgttg gcacattgac cctacaaaat
     8461 ctctctaatc tccaattttc taaagctttc gttagctgta atggtattca aaacggagct
     8521 ctagctactt ttagcgagga agagggagag
//
LOCUS       DAVMHG010000089         8545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMHG010000089 DAVMHG010000000
VERSION     DAVMHG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.089"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            77..529
                     /locus_tag="VYR08_001509"
     CDS             77..529
                     /locus_tag="VYR08_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV3467019.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(564..1952)
                     /locus_tag="VYR08_001510"
     CDS             complement(564..1952)
                     /locus_tag="VYR08_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3467020.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2018..2980
                     /locus_tag="VYR08_001511"
     CDS             2018..2980
                     /locus_tag="VYR08_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV3467021.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3186..4196)
                     /gene="ccpA"
                     /locus_tag="VYR08_001512"
     CDS             complement(3186..4196)
                     /gene="ccpA"
                     /locus_tag="VYR08_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV3467022.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4621..5220)
                     /locus_tag="VYR08_001513"
     CDS             complement(4621..5220)
                     /locus_tag="VYR08_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467023.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5222..6319)
                     /locus_tag="VYR08_001514"
     CDS             complement(5222..6319)
                     /locus_tag="VYR08_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3467024.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6319..7056)
                     /locus_tag="VYR08_001515"
     CDS             complement(6319..7056)
                     /locus_tag="VYR08_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467025.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(7058..7942)
                     /locus_tag="VYR08_001516"
     CDS             complement(7058..7942)
                     /locus_tag="VYR08_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467026.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(7929..8123)
                     /locus_tag="VYR08_001517"
     CDS             complement(7929..8123)
                     /locus_tag="VYR08_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467027.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(8120..8326)
                     /locus_tag="VYR08_001518"
     CDS             complement(8120..8326)
                     /locus_tag="VYR08_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467028.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2602 a         1969 c         1520 g         2454 t
ORIGIN      
        1 aaaaatactt tttttgcttt acttctatag tgaaaaggtt tacaattata gtaagaaaat
       61 ttcaggaggt ttcattatgg cacaacgtta ccaaaatatc atggtcgcaa tcgatggttc
      121 taaggaagcg gacttggctt ttgtcaaggg agttcattct gctctacgaa acgacgctaa
      181 actcaccatc gcacatgtca ttgacacacg cgctctccaa agcgtatcca cctttgatgc
      241 tgaagtttac gaagaactcc aagtcgacgc tgaaagtctg atgaaagagt acgaaaaacg
      301 tgctaaagat gctggggtag cagatgttca tatcgtcatt gaaatgggaa atccaaagac
      361 cctgctagca cgtactattc cagatgccga ggaagtggac ctcatcctcg ttggcgcaac
      421 tggtctcaac gcctttgaac gcctcttggt cggctcttca tctgaataca tactccgcca
      481 tgctaaggtc gatttgctgg ttgtaagaga acaagaaaaa accttataat cacaaagaaa
      541 aggagcccct agctcctttt tgtttacgat ttatttctct ctttatggcg ttcgtaagcc
      601 ttgagctggc gctgcagttc ctttttaata gcaggttctg gagcatattt ttcttcccaa
      661 ttatctggtt ttaagatttt atgggtcact ggatcaaaat gagccttacc atctggaaaa
      721 attttcccca tattggcctg atggacaata tcaaaaatac gttctgggtc cacccccatc
      781 aagacaaaac tgccgtaggt gaagtaaagc atgtcaatca aggcatccac ttgccctatc
      841 aaatcttgct gagcaggtgt cttcttggct actttatctg ctgccttatc aagggcctga
      901 tgaagttgcg atacagcttg accaaaatct tcttcagaag gactggctgc tcgaacaaac
      961 tccaccaatt cttctatttt aaaaccagcc ctatgggttg caccctctaa atcccaagct
     1021 cgaggttctt cttgggttcg ttcatccatc atgtggtgga aggtcttgac cttattgaaa
     1081 tgatagtcac ggctgacaaa gactttttct gaagacaaaa caccaaaatg ttccaaggcc
     1141 ttgtggatcc catcttgttg attgctagtg gtaatgtgct tggcagcttc cttgacactg
     1201 ctactaccat ttcccatagc gaccgacata ccaacgccag ccagcatttc aaggtcatta
     1261 tctgagtcac caaaagccat gacttggttg aggtcaaagc catattcttt cccaactcgg
     1321 cgaatgcctt ctaatttaga atttccctga ttgatgacat ccgatgcaaa aggattgcta
     1381 cgtgtcaatt tcaagtcttc aaaatcagct gccgccttct cagattcttc tggtgtcatc
     1441 agcatcaaaa cttggtaaat aggttgattc atcaggtgaa gcaggtcctc ttccttttgg
     1501 ggaacaacct tgctgaccat gcgattaaaa gactgactca ccgtccgagt taaaacagag
     1561 ggaacgaagc gactaattcg ttgggaaaaa gaacccagac caaaggacat gattttagaa
     1621 cccaacatgg catccttggt ccctaaggca atctccgtgc cctctttttt agcatagcta
     1681 attagatggc gcaaatgtaa cttggaaata gggctcgtga acaagactct gtctttacta
     1741 aagatatact ggccattata ggttaccgca aaatccagat ccaaatcgtc catcaattcc
     1801 ttaacaaaaa aaggtcctcg ccctgtcgct acgccaacta gtaccccttg ttctttgaca
     1861 atcttaatcg catccttagt ggatttcaaa acactcttgc gattgttgac caaggttcca
     1921 tcgatatcaa aaaaaacagc tttgacttcc atcctatccc aatctcccct tttgtgatac
     1981 aatgattata ccacatttca gaaagagtga gtaaatcatg cctaagaaaa tccttgtttt
     2041 acatacgggt ggaactattt ccatgcaggc cgatgcttct ggcgctgttg tgacgagttc
     2101 agataatccc atgaaccatg tgtccaaccc acttgaagga atccaagtcc acgccttgga
     2161 cttttttaac cttccaagtc cccatatcaa acccaaacat atgctggtcc tctaccagaa
     2221 aattaaagag gaagcagata actacgatgg agtggtgatc acacacggaa ccgatacttt
     2281 agaggaaaca gcctatttcc ttgataccat ggaagttccc catatgccta tcgttctaac
     2341 aggagccatg cgtagctcca atgaactcgg tagtgatggt gtttataatt acctaagtgc
     2401 tttacgagtg gccagcgatg acagggctgc tgacaaagga gttttggtcg ttatgaacga
     2461 tgaaatccac gctgccaagt atgtcaccaa aacacatacg actaatgtca gcaccttcca
     2521 gactccaaca catggccccc ttggtctcat catgaaacag gaaatcctct acttcaaaac
     2581 agctgaacct cgtgttcgct ttgaccttga tcacatacaa ggtttagtcc ctatcatctc
     2641 ggcttatgct ggtatgacag atgagctgat tgatatgctg gatttagaac acttggacgg
     2701 tttgattatc caagccttcg gagctggtaa tattcccaaa gaaacggctc aaaaattaga
     2761 aagccttctg caaaaaggaa ttccagtcgc tctggtatca cgatgcttta acggtattgc
     2821 cgagcctgtt tatgcctacc agggtggggg cgtacagttg caaaaagcag gcgttttctt
     2881 tgttaaagaa ctcaacgccc aaaaagctcg cttgaaactc ctcatcgccc tcaatgccgg
     2941 actaacagga caggctttga aagactatat ggaaggctaa tactcttcga aaatctctgc
     3001 aaaccacgtc acgtcgcctt accgtatgta tggttactga cttcgtcagt ttcatctaca
     3061 acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc tatggtattc
     3121 attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg attccctgat
     3181 tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg taagagaact
     3241 tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat actaatggca
     3301 ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa gcgtgagatt
     3361 tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacacc cttatcagcc
     3421 aaaccgttca agactcctgc tgccaactca tcacctgtca caactgctgc agttgcattt
     3481 gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt agattcaaat
     3541 accaaaccct cactataagt gattcctgct tttttcaagg tttccttgta gccaactaaa
     3601 cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat acgctcattt
     3661 tctttagcaa ggtaactcac tgcatcaatt gttgcttgct tatagtcaat attgacactt
     3721 ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg tgaacgcgaa
     3781 aattctgagc gaattttatc tgtcaagtga taccccatat agataatgcc atctacctgc
     3841 tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc gctattagct
     3901 aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc caaactcgaa
     3961 aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt tttacttgca
     4021 agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc tagcactttt
     4081 ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac cgtcgccatg
     4141 gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc attcattcct
     4201 tttcctgtcc tttctatctc acacattctt ttacaagtag aggtactgat tgaagctcta
     4261 tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac ttattccatt
     4321 ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa aaatttcata
     4381 gaaaacctag gtttagctcc ttgctaccac cttagactaa acaaaaagga ggaaactaag
     4441 ccctcctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag gacagtcaaa
     4501 tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatag
     4561 gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa tattatatca
     4621 tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac cagcatccag
     4681 ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt aaagctgatc
     4741 tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa tctcttgttc
     4801 tgtcaacgga ttgggacggg tcatcaccat ttccatcaat tcaggcgaat actccttgcg
     4861 tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt cctttaatac
     4921 ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct tgaaggtcgt
     4981 caccacaacc acctttgttt caagcttttc tgatcgtatc cactccaaga cttcaagacc
     5041 tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct tttctaatag
     5101 ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg gttgaagcgt
     5161 gagcaattga cacatggcat ctcgcaacat actttgatct tctgcgacta atactttcat
     5221 cttagtttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
     5281 ttacacttac ttctcctgaa aatggaaaaa cacgatctcg gactgtatgg agctcatctc
     5341 cttttagaaa agcaaagccg cagccatcat cacttactgt taaaatgagt tccttctctg
     5401 tccgttctaa ttttaagtaa gctttagacg ctttggcatg tttgatgata ttggtcacca
     5461 actcaagcaa aatcatagaa gccattgact ccaattcttg agtaaggcta gcagtatcta
     5521 gttggttatc cgtttccacc tcaattccag caatttctaa catctttttc acagtctcta
     5581 gttcggatgt caaagttcta gacttaagat tttccacgat agttcgaact tcattcatgg
     5641 aatccttgct gatctggtga atttctttta attccttttc cacctgtgga taagcctcca
     5701 tctgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag gtatgcccca
     5761 gactatcatg caaatcttga ccgatacgat tgcgttcatt ttcagcaagc aatagattta
     5821 tctgggcatt ttgcttagcc tgtgcttctt tcaaatcctc cacaattcga atccgaacca
     5881 agccaaaagt cattaaatcg acaaaagcaa gaattacaag tagatagaat agaaactcaa
     5941 cttcgattct ctgaaaaatc aacagttgac ccacaacaag gacttgagca agaagaaaag
     6001 tccagacatg taaagacttt aaaccaccta cgctgaaatg atagcttaag agattggata
     6061 ggaaaaagaa aaaccagata taattaacag ccacaaaggc agtattccca actacataag
     6121 tcagcaggat gccccaatat agccaagata gacgctggct cttagttgtt aaaacaccca
     6181 aatacgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc cacccagtca
     6241 ctacagttag gatggggaaa accataaaaa ttaaactgat ccaaaacata taatgtatgc
     6301 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tggtcaaacc
     6361 aaacaaaact actgaaaaaa caagtaaata aactgtggct gatagattga tgccaccctc
     6421 atttaagaag gtcttgagca actccatcaa ctgataggtc gggagacact tacctactac
     6481 ttgcatccag tctggaaata aagagatagg catccagagt ccacctaaaa cagccaaccc
     6541 tagataaaga agattgccca cgacagacat caactgacta gttggtaaga gagtcaaggt
     6601 caaaccaagc gctacgaagg caatacttcc tactatcagc aaaagtgcag ccccaatcca
     6661 atttccaaga gacatgtcca cacctcttac aaaatgccca actgagaaaa ccaccaagat
     6721 tgaaaccaaa taatcaacca gcatacttgt tatctttgat agataatatt ctaccatatt
     6781 tacagggcta tgacgcaatg ttttctgcca gttgttgatc ttgtcggtat gtaaaacaac
     6841 tgggaatgag aagatagctg ttgacatcat ggaaaatgca gtcatggaga taagataatc
     6901 acgcataaaa ttcgcgagtt cacctggtgt gtcctgatag ataccagaaa aaaataaata
     6961 gaaagccgtc ggcatcccta ccgacaatag ataatagatt aattgtcgtt tggtcaataa
     7021 aaattctatc ttactaagtg ctatccatcg tttcatctta gttatctccc ttctgcgtct
     7081 cttcaaagat tgtattcaac aaactacgat tattaacttc gatttcttgc atcctacatc
     7141 ctgcttgggc taatagttcc cagaaagcat ctgcttctcg agtgactact tgtaaagaat
     7201 cttgctttag ggtccagttt tcaaccaaat ttgactgctc tacgacttcc ttgtaagcta
     7261 taggaaggat aaagtgcttt tctatttcct cgctacgcat agctagaggc gtcgtatctc
     7321 gaatcaactc tcccttattt aagagcaaaa tgcggtctgc agtgtgttcc acttcctcaa
     7381 tataatggga cgaatagaga atggtgactc cttgcgcttt taactcctgg acaatttccc
     7441 aaaaacgttg acgagttgag gtatccatgg acgcagtagg ctcatctaaa aaaacaatct
     7501 ttggtcgccc aattaaggtc aagatgaaag aaaagagacg cttttgaccg cctgataatt
     7561 tttctgccaa ctgttctttt tgttgcttga caaactgcaa tagttgatca atttcctgat
     7621 tgctcaaagg atttggatag atacgttgaa agaaagcaat caactcttta acttttaatt
     7681 tctgaacgat tatattttct tgagggaggt aagccctagt atagtctaac ttagaactcg
     7741 tcactggtaa accttgaata gatacttgac cgcttgtgac cagtttatct ccaagcaaac
     7801 aatctaagag tgttgtcttt ccggcaccat ttggaccaat taaggcaaca cattcaccat
     7861 cgttgatttc aaaggagatg ttccgcaaaa tttccttgtc ttttattttc ttactcaatt
     7921 tttcaacttt aaccacagtc atgagactct cctttcaacc actccattcc catagggaaa
     7981 acgacgaaaa tcataaatcc aaaccccaaa gcaccacaaa tgaattggcg aagcaaggct
     8041 tgatcaaacc aatcagtaaa catttccact aaccatacca agagtgacag gccgataaag
     8101 aaatagatga tccctctctt cattcctcaa actccttttt cacatctctg actaatttca
     8161 aaccttctct aacaagccaa gacatgattc caaagccagc aaagaccttc caaggaaaat
     8221 ggtagaaatc ctcatccaat cccgaaaaca tgagataggt cataactcct gctactacta
     8281 aactcattgc aataatcatt ttatttctca tctcttcttc ccccatttca tactacaatt
     8341 atagtctttt aaaatcagag gaggcagaag cttctgtcac tagaaaatat gacaaatgtc
     8401 ataaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa tcagaaaaat
     8461 ctaaggcaac ttcctcaaaa gagatatcaa acccaattca caccataatg taaactaata
     8521 cttatttaaa atcaaaaaga gtaga
//
LOCUS       DAVMHG010000090         8467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMHG010000090 DAVMHG010000000
VERSION     DAVMHG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.090"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..433
                     /gene="gpsB"
                     /locus_tag="VYR08_001519"
     CDS             104..433
                     /gene="gpsB"
                     /locus_tag="VYR08_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV3467029.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            454..834
                     /gene="rnpB"
                     /locus_tag="VYR08_001520"
     ncRNA           454..834
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYR08_001520"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            919..2076
                     /locus_tag="VYR08_001521"
     CDS             919..2076
                     /locus_tag="VYR08_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV3467030.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            2088..3482
                     /gene="mapZ"
                     /locus_tag="VYR08_001522"
     CDS             2088..3482
                     /gene="mapZ"
                     /locus_tag="VYR08_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV3467031.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            3558..4982
                     /gene="gndA"
                     /locus_tag="VYR08_001523"
     CDS             3558..4982
                     /gene="gndA"
                     /locus_tag="VYR08_001523"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV3467032.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            4994..5683
                     /locus_tag="VYR08_001524"
     CDS             4994..5683
                     /locus_tag="VYR08_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467033.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            5781..6800
                     /gene="cbpC"
                     /locus_tag="VYR08_001525"
                     /pseudo
     CDS             5781..6800
                     /gene="cbpC"
                     /locus_tag="VYR08_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
     gene            complement(6918..8150)
                     /locus_tag="VYR08_001526"
     CDS             complement(6918..8150)
                     /locus_tag="VYR08_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3467034.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
BASE COUNT         2643 a         1504 c         1967 g         2353 t
ORIGIN      
        1 aaatgaactt gctgaaaatt tttccgaaaa ttgaagcttt gaccttgatt tttgtttgcc
       61 tttttttata taataatact agcaagcgag aatggagaga gacatggcaa gtattatttt
      121 ttcagcgaaa gatatttttg aacaagagtt tggacgtgaa gtccgtggct ataataaagt
      181 agaagttgac gagtttttag acgatgtcat caaggactat gaaacctatg ctgccttggt
      241 caagtcactt cgtcaggaaa ttgcggattt gaaggaagaa ttaactcgta aaccgaaacc
      301 ttcaccagtt caagcagaac cccttgaagc ggcaattaca agttctatga cgaattttga
      361 tattttgaaa cgcctgaata gattggaaaa agaagttttt ggtaaacaaa ttttagataa
      421 ctcagatttt taagtagtta tttgagatgt gcaatttttg gataatcgcg tgaggagaat
      481 tgcttctcat gaggaaagtc catgctagca caggctgtga tgcctgtagt gtttgtgcta
      541 ggcgaaacca taagcctagg gacgagaaat cgttacggca gttgaaatgg ctaagtcctt
      601 ggataggcca gagtaggctt gaaagtgcca cagtgacgga gtctttctgg aaacagagag
      661 agtggaacgc ggtaaacccc tcaagctagc aacccaaatt ttggtcgggg catggagtac
      721 gcggaaacga acgtagtatt ctgactgcta tcagctagag ctgttagtgg tagacagatg
      781 attatcgaag gaagtggtcc tagtcacttc tggaacaaaa catggcttat agaaaattgc
      841 atataggttg gggctgagaa attttctcaa cctcattttt taaagtggac atatagaaag
      901 gtcttgcaag actgtaacat gaaaaaagaa tttaatttaa ttgcaactgt ggcagcaggg
      961 cttgaggctg tcgttggtcg tgaagtgcga gagttgggct acgattgtca gattgaaaat
     1021 ggacgtgttc gttttcaagg agacgtgaga gctattatcg aaaccaacct ttggctgcgg
     1081 gcagcagatc gtatcaaaat tatcgtagga acgttccctg ctaagacttt tgaagagcta
     1141 tttcagggag ttttcgcttt agattgggaa aattatttac cacttggagc tcggttcccg
     1201 atttcaaaag ctaaatgtgt taagtccaaa cttcacaatg agcccagtgt tcaggctatt
     1261 tctaagaaag ctgttgtcaa gaaattgcag aaacactatg ctcgcccaga aggggttcct
     1321 ctgatggaga atggcccaga gtttaagatt gaggtctcta ttctcaaaga tgtggcaact
     1381 gtcatgattg atacgaccgg gtctagcctc tttaaacgtg gttatcgtac cgaaaaaggt
     1441 ggcgctccta tcaaggaaaa tatggcagca gccattttac aactttctaa ctggtatcca
     1501 gacaagcctt tgattgatcc gacctgtggt tcggggactt tctgtattga ggcagttatg
     1561 attgctagaa agatggcgcc aggtcttcgt cgctcttttg catttgagga atggaactgg
     1621 atcagcgatc gcttgattca agaagtgcgc acagaagcgg ctaaaaaagt agaccgtgag
     1681 cttgagctgg atatcatggg ctgtgatatt gatgctcgca tggtggaaat tgctaaggcc
     1741 aatgctcagg tagctggtgt tgcaggagac attactttta agcagatgcg cgtgcaggat
     1801 ttacgttccg ataaaatcaa tggagtaatc atttccaatc cgccttatgg tgaacgtttg
     1861 tcagatgatg caggggtgac caagctctat gctgagatgg ggcaagtatt tgcaccgctg
     1921 aaaacttgga gcaaatttat cctgactagt gatgaagctt ttgaaagcaa gtatggtagc
     1981 caagcagata agaagcgtaa gttatacaac ggaaccttga aagtggatct atatcaatat
     2041 tttggtcagc gtgtcaaacg gcaagaggta aaatagaaag gatactcatg agtaaaaaaa
     2101 gacgaaatcg tcaaaaaaaa gaaggtcaag aaccgcaatt tgattttgat gaagcaaaag
     2161 agctaacagt tggtcaagct attcgtaaaa atgaagaagt ggaagcagga gtcttgcctg
     2221 aggattccat tttggacaag tatgttaagc aacacagaga tgaaattgag gcggataagt
     2281 ttgcgactcg tcaatacaaa aaagaggagt tcgttgaaac tcagagtctg gatgatttaa
     2341 ttcaagagat gcgtgaggct gtagagaagt cagaagcttc ttcggaggaa gttccatctt
     2401 ctgaagacat cttactaccc ttgcctctgg acgatgagga gcaaggcttg gatcctctat
     2461 tgctagatga tgaaaatcca acagaaatga ctgaagaagt ggaagaggag caaaaccttt
     2521 ctcgtctgga tcaagaggac tcagaaaaga aaagtaaaaa aggctttatt ttgaccgttt
     2581 tggcgcttgt atcagtaatt atttgtgtca gtgcttatta tgtctaccgt caagtggctc
     2641 gttcgaccaa ggaaattgaa acttctcaat caactacagc caatcaatcg gatgtggatg
     2701 attttaatac actttatgac gccttttaca cagatagcaa taaaacggct ttgaaaaata
     2761 gccagtttga taaactgaat caactcaaga ctttacttga taagctggaa ggtagtcgtg
     2821 aacatacgct tgccaaatct aaatatgata gtctagcaac gcaaatcaag gctattcaag
     2881 atgtcaatgc acaatttgaa aaatcagcta ttgtggatgg tgtgttggat accaatgcca
     2941 aagccaaatc ggatgctaaa tttacagata ttaaaactgg aaatacggag cttgataaag
     3001 tgctagataa ggctatcagt cttggtaaga gccagcaaac aagtacttct agctcaagtt
     3061 taagtcaaac tagcagctca agttcaagtc aagcaagttc aaatacgact agtgagccaa
     3121 aaccaagtag ttcaaatgag actagaagta gtcgcagtga agtcaatatg ggtctctcga
     3181 gtgcaggggt tgctgttcaa agaagtgcca gtcgtgttgc ctataatcag tctgctattg
     3241 atgatagtaa taactctgcc tgggattttg cggatggtgt cttggaacaa attctagcga
     3301 cttcacgttc acgtggctat atcactggag accaatatat ccttgaacgt gtcaatatcg
     3361 ttaacggcaa tggttattac aacctctata agccagatgg aacctatctc tttaccctta
     3421 actgtaagac aggctacttt gtcggaaatg gcgctggtca tgcggatgac ttggactact
     3481 aagcagtcgt tacaaaattc tttcttttca aaagtaaaaa tgataaaata aaacaaatga
     3541 aacaagagga gtgtcaaatg acaaaagcta actttggtgt cgtaggtatg gccgtaatgg
     3601 gtcgtaacct tgcccttaat attgaatcac gtggttacac agttgctatc tacaaccgta
     3661 gtaaagaaaa aacggaagat gtgattgctt gccatcctga aaagaatttt gtaccaagct
     3721 atgacgttga aagttttgta aactcaatcg aaaaacctcg tcgtatcatg ctgatggttc
     3781 aagctggacc tggtacagat gctactatcc aagcccttct tccacacctt gacaaaggtg
     3841 atatcttgat tgacggtgga aatactttct acaaagatac catccgtcgt aatgaagaat
     3901 tggcaaactc aggtatcaac tttatcggta ctggagtttc tggtggtgaa aaaggtgctc
     3961 ttgaaggtcc ttctatcatg cctggtggac aaaaagaggc ctacgaattg gttgcggatg
     4021 ttcttgaaga aatctcagct aaagcaccag aagatggcaa accatgtgtg acttatatcg
     4081 gtcctgatgg agctggtcac tatgtgaaaa tggttcacaa cggtatcgag tacggtgata
     4141 tgcaattgat cgcagaaagt tatgacttga tgcaacactt gctaggtctt tctgcagagg
     4201 atatggctga aatctttact gagtggaaca agggtgaatt ggatagctac ttgatcgaaa
     4261 tcacagctga tatcttgagc cgtaaagacg atgaaggcca agatggacca atcgtagact
     4321 acatccttga tgctgcaggt aacaagggaa ctggtaaatg gactagccaa tcatctcttg
     4381 accttggtgt accattgtca ctgattactg agtcagtgtt tgcacgctac atttcaactt
     4441 acaaagaaga acgtgtatat gctagcaagg tgcttccaaa accagctgcc ttcaactttg
     4501 aaggagacaa ggctgaattg attgaaaaga tccgtcaagc cctttacttc tcaaaaatca
     4561 tttcatacgc acaaggattt gctcaattgc gtgtagcctc taaagaaaac aactggaact
     4621 tgccatttgc agatatcgca tctatctggc gtgatggctg tatcatccgt tctcgttttt
     4681 tgcaaaagat tacagatgct tacaaccgcg atgcagatct tgccaacctt cttttggacg
     4741 agtacttctt ggatgttact gctaagtatc aacaagcagt gcgtgatatc gtagctcttg
     4801 cggttcaagc tggtgtgcca gtgccaactt tctcagcagc tattacttac tttgatagct
     4861 accgttcagc tgaccttccg gctaacttga tccaagcaca acgtgactac tttggtgctc
     4921 acacttacca acgtaaagac aaagaaggaa ccttccacta ctcttggtat gacgaaaaat
     4981 aagtaggtca gccatgggga aacggatttt attacttgag aaagaacgaa atctagctca
     5041 ttttttaagt ttggaactcc agaaagagca gtatcgggtt gatctggtag aggaggggca
     5101 aaaagccctt tccatggctc ttcagacaga ctatgatttg attttattga acgttaatct
     5161 gggagatatg atggctcagg attttgcaga aaaattgagt cgaactaaac ctgcctcagt
     5221 catcatgatt ttagatcatt gggaagactt gcaagaagag ctggaagttg ttcagcgttt
     5281 tgcagtttca tacatctata agccagtctt tatcgaaaat ctggtagcgc gtatttcggc
     5341 gatcttccga ggtcgggact tcattgatca acactgcagt ctgatgaaag ttccaaggac
     5401 ctaccgcaat cttaggatag atgttgaaca tcacacggtt tatcgtggtg aagagatgat
     5461 tgctctgacc cgccgtgagt atgacctttt ggcgacactt atgggaagca agaaagtatt
     5521 gactcgtgag caattgttgg aaagtgtttg gaagtatgaa agtgcgaccg agacaaatat
     5581 cgtagatgtc tatatccgct atctacggag caagcttgat gttaaaggtc aaaaaagcta
     5641 cattaaaacc gtgcgtggtg ttggttacac catgcaagaa tagaaaagca gttgcagttg
     5701 tgtaactgct tttttgagga gtttttatat attgacatgc agtcgggtct ttgctacaat
     5761 cagttatgga ggatatataa atgaaacttt tgaaaaaaat gatgcaagtt ctactagcag
     5821 tctttttctt tggtttgcta gctacaaata cggtatttgc aaataccaca ggtggccgat
     5881 ttgttgataa ggataataga aaatattatg taaaagatga tcataaagca atctattggc
     5941 ataaaataga cggtaaaact tactattttg gtgatattgg agagatggtt gtcggttggc
     6001 aatacttaga aattcatgga acaggttatc gtgataattt attcgataac caaccagtta
     6061 atgaaattgg ccttcaggag aagtggtact attttggaca agatggtgct ttgctagaac
     6121 aaacagataa acaagtacta gaggcaaaaa cgtctgaaaa tacaggaaaa gtatacggtg
     6181 aacaatatcc tctatctgct gaaaagagaa cttattattt tgataataat tatgctgtaa
     6241 agacaggctg gatttatgaa gacggcaatt ggtattattt aaataagcta ggaaattttg
     6301 gcgatgattc ttacaatcta ctaccaattg gtgaagttgc taagggttgg actcaagatt
     6361 ttcatgttac tattgacatt gatagaagca aacctgctcc atggtactac ctagacccag
     6421 aaactggcat tatgtgaaca ggatggcaat atctaggtaa taactggtac tacctccgtt
     6481 catcaggagc aatggccact ggctggtatc aggaaggtac cacttggtat tatttagacc
     6541 agccaaatgg cgatatgaaa acaggttggc aaaaccttgg gaacaaatgg tactatctcc
     6601 gttcatcagg agctatggca actggttggt atcaagatgg ttcaacttgg tactacctaa
     6661 atgcaggtaa tggagacatg aagacaggtt ggttccaggt caatggcaac tggtactatg
     6721 cttatagctc aggtgctttg gcagtgaata cgaccgtaga tggctattct gtcaactata
     6781 atggcgaatg ggttcggtaa tgacagggcg cttataatta tattagaaca ctcgcttaat
     6841 caaagaagtg actttgttgt acttgacctg acgattgggg agattaaaag catgtgaaat
     6901 ctacacatgc tttttcgtta gttatgagtg ttgaacttga ctaaccattg acaaaagata
     6961 agagcaatta aaataagata caaaaatatc aatgctaact gcaccgaaac gcccgatgct
     7021 atggataggg ctaataggga gagcagggac ggggcgagaa tgtccactgt gctcagagca
     7081 gagcccaccg tccccatctt gtcactgggg atataggcaa agacagctgc ttgtaggcga
     7141 gggctgagaa tgccgataat cgtagaactg atgaaactgg tcatgaaaat tacgatatag
     7201 gcttgacaaa ggatactagt tattagagcc aatagggaaa tctcacagaa aaccaagagg
     7261 tgcgtgaaac ggatattttt aaatagatta ctgctggtca tattgccaag gatagctcca
     7321 caagacatga tgatggagag cagggcgagg agttgcccaa tgctcagttg tgctagtgtc
     7381 tgttggatga agcggagggc aatcagccga ggggacacat ccaagatggc aatcatactg
     7441 gtcgtcagaa acagtagtat gacggtatct tttaacttca gtagcacagc cagcgactgt
     7501 ttaagatgtt gaaaatattt cttaaaactc agtgctgtat gtgacatttc aatccttttt
     7561 tcaacctcat acaaggtatg tcgaataaca tacaggccca aaaaggcaat gacaaaagtc
     7621 agagtgttga taagggaaat agtttgaata cttataacat tgataaggaa tgcgccagcc
     7681 agattggcga caatgcggac aaccctcatc agagactgcc tgaaccccat ggcatcatgc
     7741 aggtcgtcct taattacact gatgtagagg gcgttcatca tgtaggcaga caggtagctc
     7801 aaggtgtcag agatgaggtt gatgatgaca atcataatga ctgaaactaa tgaaaagggc
     7861 gacaagacga gaaatactgt caaaatagcg tagagcagga ttttgatttt ggcaatacag
     7921 agtgcgtgtt tgactctatt ttcttgaaaa tctcctagaa tcccgagaaa gagttgcgat
     7981 agtaggggaa ccatctctga aatggaaatc agtaaaatcg ctagcggagc gaaattgtac
     8041 tgagccacat aattgataat ggcgaggtag aaaatactgt cgccgaatga tgaaaaacag
     8101 ctgtttagca gcaactgtct atatatgttg tttgtccaaa ataatttcat acatttctcc
     8161 ttatgtctgc acatcgaatg ggaatttatt atttaataga caccaatata cccccatgct
     8221 tcctattccg aagtcaaaca aatctgcttt accactatct ccttgaaaca tcagtaaacc
     8281 gtgatcatct cgttcagaac atgctaaaat tacctgttgg attttttcat cagtaatttg
     8341 ttttgattgt agacagtggt ttgtagtaag ctagttattc cgtggcaata tcctgtcatc
     8401 atttttagat gatggttaaa aacaaattct tgatatttac taataatatc tttgtttccg
     8461 tcacaat
//
LOCUS       DAVMHG010000091         8457 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMHG010000091 DAVMHG010000000
VERSION     DAVMHG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.091"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            224..721
                     /locus_tag="VYR08_001527"
     CDS             224..721
                     /locus_tag="VYR08_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV3467035.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            808..1200
                     /locus_tag="VYR08_001528"
     CDS             808..1200
                     /locus_tag="VYR08_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3467036.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1184..3631
                     /locus_tag="VYR08_001529"
     CDS             1184..3631
                     /locus_tag="VYR08_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV3467037.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3634..5811
                     /locus_tag="VYR08_001530"
     CDS             3634..5811
                     /locus_tag="VYR08_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV3467038.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            5808..6809
                     /locus_tag="VYR08_001531"
     CDS             5808..6809
                     /locus_tag="VYR08_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV3467039.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            6806..7738
                     /locus_tag="VYR08_001532"
     CDS             6806..7738
                     /locus_tag="VYR08_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3467040.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            7983..8099
                     /locus_tag="VYR08_001533"
     CDS             7983..8099
                     /locus_tag="VYR08_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV3467041.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8115..>8457
                     /locus_tag="VYR08_001534"
     CDS             8115..>8457
                     /locus_tag="VYR08_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV3467042.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFH"
BASE COUNT         2668 a         1594 c         1787 g         2408 t
ORIGIN      
        1 attggctttc tcattattgc gattattgcg gtaggcttag tcttcaacgc tgctggagta
       61 aaagacattt tactagagct attcaatcgc attattggtg cttaaattaa accgttattt
      121 tgtggaatat aagtggtttt cttatgttcc gcaaaagaat ggtacaccaa acgaagtgcg
      181 gtagggattt ttgaatctct ataaagaaag gacgtgtgaa tacatggacg atatgcaagt
      241 ctacattgcc aatttaggca aatacaatga gggcgaatta gttggtgcat ggtttacctt
      301 tcccattgat tttgaggaag tcaaagagaa aatcggcttg aatgatgaat atgaggaata
      361 cgccattcat gattacgagt taccctttac ggttgacgaa tacacttcta ttagcgaact
      421 caatcgccta tgggaaatgg tatcggagtt gccagaagaa ctacaatcgg agctatctgc
      481 tctgctcact catttttcaa gtattgaaga attaagcgaa catcaagaag atattatcat
      541 tcattccgat tgtgatgata tgtctgacgt ggcacgctac tacattgaag aaacgggtgc
      601 tttaggcgaa gtgccagcca gtcttcaaaa ctatattgat tatgaatcct atggtcggga
      661 tttagacctt tcgggaacgt ttatctcaac caatcacggg atttttgaaa tcgtctatta
      721 aatctgtcgg tacattctta ctggcagatt ttcttgttta cggggtagtc aaaagactat
      781 ccccattttt atgaaaggat tgattacatg aagaaaatac gaagctatac cagtatctgg
      841 tctgttgaga aagtgttgta ctccatcaat gactttagac ttccgtttcc cataaccttt
      901 acgcaaatga catggtttgt cgtgtcactc tttgcagtta tgatacttgg caacttgccc
      961 cctctttcta tgatagaggg agcatttctc aaatactttg ggattcctgt ggctttcaca
     1021 tggtttatgt ctacaaaaac ctttgatggt aaaaagcctt atggattttt gaagtctgtc
     1081 attgcttatg cactgcgacg aaaactgacc tatgcaggaa aaaaagtaac gcttggcaga
     1141 aaccagccac aagaagccat tacagcagtt aggagtgaat tttatggcat atccaattaa
     1201 atatattgaa aacaatctgg tctggaataa agatggggaa tgttacgctt actatgagct
     1261 tgttccctac aattactcat ttctaagtcc ggaacagaaa atacaagtac atgattcttt
     1321 cagacagctt atcgcacaaa atcgtgatgg caagattcat gctttacaaa tcagtacaga
     1381 atccagcata ctttctgcac aagaacgttc caaaaatgaa gtcactggaa agctcaaaac
     1441 ggttgcctat gacaaaatcg accaacagac agacgcttta atatccatga ttggcgaaaa
     1501 tcaagtggac taccgtttct ttatcggttt taagttgctt ctcaacgatc aggagttttc
     1561 tatgaaaagt cttaccgttg aagcaaaaaa tgcttttact gattttgtct atgatgtgaa
     1621 ccataagctg atgggcgatt ttgttagtat gagtaatgat gaaatcctgc gttttcagaa
     1681 gatggaaaag cttttagaaa ataaaatctc ccgtcgtttc aaaatccgaa gattagataa
     1741 ggacgacttc ggctatctga ttgaacacct ttacggacag acaggcactg cctatgaaga
     1801 gtatgagtat cctctatcaa agaaaaagct ggaacatgaa acactgatta aaacctatga
     1861 cctcattaag cctactcgct gtctggtgga agaaaaacag cgatatttga aaatccagca
     1921 ggaagacgaa accgcctatg tagcttactt taccatcaac agtattgtcg gcgaactgga
     1981 cttcccgtcc tctgaaatct tctactacca gcaacagcaa tttacctttc cgattgatac
     2041 gtcaatgaat gtggaaattg tagcgaatcg taaagcccta tctactgtcc gcaataaaaa
     2101 gaaagaactg aaagacttgg ataaccacgc atggcaaagt gataatgaaa ccagctctaa
     2161 tgtggcggaa gctctggaaa gtgtgaacga gctggaaacc aatttagacc aaagcaagga
     2221 atctatgtat aagctgtcct atgttgtaag ggtatcagca aatgaccttg acgaacttaa
     2281 acgtcgttgt aatgaagtga aagatttcta tgatgatttg agcgtgaaac tggtacgacc
     2341 ttttggggat atgctgggct tacatgaaga atttttacct gccagcaaaa gatatatgaa
     2401 tgactatatt caatacgtga cctctgattt cctcgctggt ttaggttttg gtgctactca
     2461 aatgctgggt gaaaatgagg ggatttatgt tggctacagc ttagatactg gacgcaatgt
     2521 ctatctgaaa cctgctcttg ccagtcaagg ggttaagggt tcagtaacca atgcgttagc
     2581 gtctgccttt gtcggttcgc tgggtggtgg taaatccttt gcgaataacc ttatcgtcta
     2641 ttatgcagtg ctttatgggg cacaagcagt gattgtagac ccaaaagcag aacgtggcag
     2701 atggaaagaa accttgccag agatttccca tgaaatcaat atcgtcaatc tgacttctga
     2761 tgagaaaaac aaaggcttac ttgaccctta tgtaattatg aaaaatacca aagattctga
     2821 atcactggct attgatattt tgacattcct tacggggatt tcctctcgtg atggggaacg
     2881 cttcccaatc cttagaaaag ccattcgtgc agtaaccaat agtgaagtgc gagggttgat
     2941 gaaagtgatt gaagaattac gggttgagaa tacgccacta agtaccagta tagccgacca
     3001 tatcgaaagt tttacagact atgactttgc acatttatta ttcagtaatg gttatgtgga
     3061 gcagtctatc agcttagaaa aacaactgaa cattatacag gttgcggact tggtacttcc
     3121 cgacaaggaa acttcctttg aggaatatac cactatggag cttttatccg ttgctatgct
     3181 gattgtcatt agtacctttg ctttagactt tatccataca gaccgaagca ttttcaagat
     3241 tgtagattta gacgaagcat ggagcttttt acaggtagca caaggaaaaa cactatctat
     3301 gaagctggtt cgggctggtc gtgctatgaa cgctggggta tatttcgtga cccaaaatac
     3361 agacgacctc ttagatgaaa aactgaaaaa taacctcggc ttaaaatttg catttcgttc
     3421 cactgacctt aacgagatta aaaagacctt agcctttttt ggtgtagacc cagaggacga
     3481 aaacaatcag aagcgattgc gtgatttgga aaacgggcaa tgccttatca gtgatttata
     3541 tggtcgtgtc ggtgtgatac agttccaccc tgtatttgaa gaactgctcc atgcctttga
     3601 taccagacca cctgtgcgaa aagaggtgta aatgtgaaac catcaatagt aaacagaata
     3661 aaatcaaact ggacgctgaa acgtctaggt aaagtggcaa tgacagtggc tttcacactt
     3721 gtgattgcca tttttctttt agccatgctg ggaacggtgg ttcaagctgc gggcttggta
     3781 gatgatacgg tcaatgtggc aaatgaatac agccgatacc cacttgaaaa ctatcaactg
     3841 gatttttatg tggataatag ctggggctgg cttccgtgga actggtcgga cgggattgga
     3901 aaacaggtca tgtatggact atatgccatt accaatttta tttggacaat cagtttgtat
     3961 gtttccaatg cgacaggtta cttagtacag gaagcctatt ccttagactt catttccgct
     4021 acagcagatt ccattggtaa gaatatgcag accttagctg gtgtgagtgc aaacggattt
     4081 tcaacagagg gtttctatgt tggattcctc ttactcttga ttttggttct tggggtttat
     4141 gttgcctata cgggactgat aaagagagaa accacaaagg caattcatgc cattatgaat
     4201 tttgtgctgg tgtttatcct atcggcttcc tttattgcct acgctcccga ctacattaaa
     4261 aaaatcaatg acttttcatc agacatcagt aatgccagtt tatcacttgg cacgaagatt
     4321 gtcatgcccc attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg
     4381 ttttccatac aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa
     4441 agtatcggta ttgaccgtgt ggaaagcctg ctctccacca gcccagattc caacaatggc
     4501 gaagatagag aaaaaattgt tgcggaagaa attgaagaca gaagcaatac caatctaacc
     4561 attacaaaga ccattaaccg tttaggtaca gtcttcttcc tatttgtctt caatattggg
     4621 atttccatat ttgtattcct attaacagga atcatgattt tctcgcaggt actttttatc
     4681 atctatgcta tgtttctgcc tgtgagcttt attttaagca tgattccatc atttgatggt
     4741 atgtcaaaac gagccataac aaagctcttt aataccattt tgacacgagc tggaatcaca
     4801 ttgattatta cgacagcatt tagtatttca accatgctct ataccttatc ggctggttat
     4861 ccgttctttt tgattgcttt tctacagatt gtgacctttg caggaatcta cttcaagctg
     4921 ggcgatttaa tgagtatgtt ttctctacag agtaacgatt ctcaaagtgt gggaagtcgt
     4981 gtgatgagaa aacctcgtat gcttatgcac gctcacatgc accgtctaca gcggaaactt
     5041 ggacgttcca tgactactct aggggctggg tctgccattg ttacaggtaa aaaaggacag
     5101 tcgggttcgg ggagttctgc aaggacacaa gcagatcact cccgaccaga cggaaaggaa
     5161 aaatcaacac ttggaaaacg tatcggtcaa accatcggta cagtagctga taccaaagac
     5221 agaatggtag acactgctag tggtttgaaa gaacaggtta aagatttgcc gaccaatgca
     5281 agatatgcag tatatcaagg aaaatccaaa gtaaaagaga atgtccgtga tttaaccagt
     5341 agtatttctc aaaccaaagc ggacagagcc agtggacgca aggaacagca ggaacaaagg
     5401 cgaaaaacca ttgcgaagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcct
     5461 gcttcttctg ttcatgaaag accgactaca agacaagaac aatatcatga tgaacagacc
     5521 tcaaaacagt ctaatattca gacttcatat aaggaatctc aacaagccaa acaagagcgt
     5581 ccagcagtta agtccgattt ttcaagtcca aaagtggaac gccaaggcaa taccgttcaa
     5641 gaaaaaaccg ttcaaaagcc agcaacttca accactacag cagatagaac ttcacaacgt
     5701 ccaatcacaa aagaacgtcc gtctactgtt caaagagtac cactacaaaa tacaagaagt
     5761 agaccaccaa tcaaaaccgc caccattaag aaagtcggta agaaaccatg aagttgaaaa
     5821 ctttagtgat tggtggttct ggattattct tgatggtctt ctcactgctt ctgtttgttg
     5881 ccattttatt ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga
     5941 atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg
     6001 gcgttgaaga atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg
     6061 cggaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag
     6121 aagaatccat taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc
     6181 tcagtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt
     6241 atgtggctaa tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt
     6301 attcaggtgg cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct
     6361 ggcgatacaa ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa
     6421 cagagtttga tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct
     6481 ggcgatacgt ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac
     6541 aatggacgta tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg
     6601 tgacccagca tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta
     6661 cctataacgc tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt
     6721 ttcatgcagg cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt
     6781 tagtgggagc aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa
     6841 aaatcagaat aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa
     6901 tcctcataaa aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc
     6961 gatattcaag cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga
     7021 aaaggaatac gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta
     7081 ctattcatgg gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta
     7141 tctgacagat gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc
     7201 gtcttctgta agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa
     7261 tgtaacctac agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc
     7321 tgcttatata gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc
     7381 gaccattacc aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg
     7441 gacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
     7501 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
     7561 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa
     7621 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
     7681 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
     7741 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
     7801 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
     7861 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtag gaacaaataa
     7921 ttggatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc
     7981 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
     8041 aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa
     8101 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa
     8161 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
     8221 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
     8281 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
     8341 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
     8401 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatg
//
LOCUS       DAVMHG010000092         8387 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMHG010000092 DAVMHG010000000
VERSION     DAVMHG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.092"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(35..1015)
                     /gene="dusB"
                     /locus_tag="VYR08_001535"
     CDS             complement(35..1015)
                     /gene="dusB"
                     /locus_tag="VYR08_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV3467043.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1002..1874)
                     /gene="hslO"
                     /locus_tag="VYR08_001536"
     CDS             complement(1002..1874)
                     /gene="hslO"
                     /locus_tag="VYR08_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV3467044.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2039..3466
                     /locus_tag="VYR08_001537"
     CDS             2039..3466
                     /locus_tag="VYR08_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467045.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            3625..5133
                     /gene="glpK"
                     /locus_tag="VYR08_001538"
     CDS             3625..5133
                     /gene="glpK"
                     /locus_tag="VYR08_001538"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV3467046.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5175..7001
                     /gene="glpO"
                     /locus_tag="VYR08_001539"
     CDS             5175..7001
                     /gene="glpO"
                     /locus_tag="VYR08_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV3467047.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7071..7775
                     /locus_tag="VYR08_001540"
     CDS             7071..7775
                     /locus_tag="VYR08_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV3467048.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8072..8275
                     /locus_tag="VYR08_001541"
     CDS             8072..8275
                     /locus_tag="VYR08_001541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467049.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
BASE COUNT         2361 a         1680 c         1977 g         2369 t
ORIGIN      
        1 aatattatac ttttgtccta ggctcttttt tgcattatac tttttccaat tgcaagaggg
       61 cttcaatctc tgccagggtg ctagcttgcg aaatggctcc acggagtttg gcagcgccag
      121 atgttccccg gagatagtga ggagcgaggc cgcggaattc acgaactgcg acgttttctc
      181 ctttgaggtt aatcaatcgt ttcaagtgtt cgtaggcgat cttcatcttg tcttcaaagg
      241 tcaaatcagg taggatttct cctgtttcaa agtaatggtt gatttggttg aagaggtaag
      301 gatttcccat ggcagctcgg ccaatcatga ctgcgtcagc accaacttct tcgatgcgtt
      361 gcttggcttc ttggacagta cggatatcac cgttggcgat gaatggaatc ttggttagag
      421 cttgggcaac cttgtaaagg gtctcaaggt ctgcgtggcc agtatacatt tgttcacggg
      481 tacggccatg catggcgagg gcagaaacac ctgcagcctc agcagcgagg gcattttcta
      541 ctgcaagaga tgggtccgcc cagccggtac gcattttgac agtaagtggg atatcaagga
      601 cagactggac cttgttgatg atagagtaga tcttgtcagg atccttgagc cacatagctc
      661 cagcttcgtt cttcacgatt ttgttgacag ggcagcccat gttgatatcg acgatatcgg
      721 tcttggtgtt ttcttggatg aattctgctg cgcgtgctag gctgtcttca tcgctaccaa
      781 aaagttggat agagacaggg ttttcgccct catcgatatg aagcatgtgc agggtttttt
      841 cgttgttgta ttggattccc ttgtcagaga ccatttccat tacaacgagt ccagctccga
      901 gctcttttgc gatggtacga aaggctgagt tggtcacgcc agccataggc gctaaaacgg
      961 tacgattggg aatctcaaca ttgccaatca taaaaggtgt attaagattt gtcacgaatg
     1021 agttcctcca ggtccttttc atcaaagttg taagtagttt ggcagaattg acaagtgatt
     1081 tctaccccgt ggtcttcctc tttcatttcc tgtaagtctg agcttggaag gctggcaaga
     1141 gcgttcataa agcgttcatg gctacagtca cattggaaac ggatttcttc ttcagaaaga
     1201 cgcttgtagg cttcgtcccc gtagatagcc ttgaggaggg cttcgatatg gtcgtcgctt
     1261 tcgagaagag tagagatagc tggcatttct tggatgcgtt tttcaaagcg agcaatctct
     1321 tctttcttgg ctcctggcaa gacttgaact aggaaaccac ctgcaatctt gaccttgtct
     1381 tcctcgtcca aaaggacatt gaggccgacc gctgaaggcg tttgttggct ttcagtaagg
     1441 taaaaggcaa ggtcttcacc gatttctcca gagatgaggg gagttataga gttgtaagga
     1501 tttccagtac cgtagtctgt gataacgagg aattgaccat ttccaacaaa aggtccgact
     1561 aggacttcac cagtcgcagt ctttttgatg tcaacaccag gattttgaac gtagcctttg
     1621 acgttcccct tggtatcagc gacggtgata atagcaccta gagagctaga tcccaacacc
     1681 ttaactgtaa gtttggtatt tcctttttca ttggctgcga gaatctggct ggcgataaga
     1741 gttcgaccaa gcgctacagt tgagctagct tgggtttgat gtttttcttg agcagtgcgg
     1801 acggtttcag tgctatcaag gacaaaagca cgaaaggctc cgctttctga tatagtttta
     1861 ataattttat ccatagctac tattttagca taaaaatgcc caaaggggga gccgtgtgtt
     1921 tactgatttt caggataatg gaccaggaaa tcagcatgaa aataaaaaga gaaacagatt
     1981 atttcagcat ttgtaagatt tatgctatgc ttaaggtaga aaatgaaagg ggtaacaaat
     2041 gtatttagga gatttgatgg agaaagccga gtgtggtcaa ttttcaatcc tttcctttct
     2101 attacaagag tctcagacga ccgtcaaggc tgtaatggaa gaaacaggat tttcaaaagc
     2161 aaccctaacc aaatatgtca ccctgctcaa tgacaaggct ttggatagtg gcttagaact
     2221 gactattcac tcagaagatg aaaatctgcg tctgtctatc ggtgcagcta ccaaggggag
     2281 agatattcgg agcttgtttt tggagagtgc tgttaaatac cagattttgg tttatctttt
     2341 ctaccaccaa cagtttttag cccatcagct ggctcaagaa ttggtgatta gcgaggctac
     2401 gcttggtcgt cacttggctg gtttaaatca gattttgtca gaatttgatt tatccatcca
     2461 aaatggacgt tggcgaggtc cagagcatca gattcgctat ttctatttct gtcttttccg
     2521 aaaggtctgg tcgagtcagg aatgggaagg tcacatgcag aaaccagaga gaaaacagga
     2581 gattgccaat ttagaggaaa tctgcggtgc aagtttgtct gtggggcaga aattggactt
     2641 ggttctctgg gctcacatca gtcaacaacg tcttcgggtc aatgcttgtc agtttcaagt
     2701 catagaagag aaaatgcgag ggtattttga caatatcttc tatcttcgtt tgctgagaaa
     2761 ggttccgtcc ttttttgctg ggcaacatat tccactagga gttgaggatg gtgagatgat
     2821 gatattcttc tcttttctcc tatctcatcg cattcttcct cttcatacta tggagtatat
     2881 tcttggtttt ggagggcagt tggcagattt actgacgcaa ttgattcaag aaatgaagaa
     2941 ggaggaacta ttgggggatt atacagagga ccatgtcacc tatgaactca gtcagctttg
     3001 tgctcaagtc tatctctata agggctatat tttacaggat cgctacaagt accagttaga
     3061 gaatcgtcat ccatatttac tgatggaaca tgattttaaa gagacagcag aggagatttt
     3121 tcatgctcta cctgcttttc aacaggggac agatttagat aagaagattc tctgggaatg
     3181 gctccagtta atcgaatata tggctgaaaa cggtggtcag catatgcgga ttggtctgga
     3241 tttgacatct ggttttcttg tcttttcaag gatggcagcc attttgaaac ggtatttgga
     3301 atacaatcgt tttattacca ttgaagctta tgaccctagt cggcattatg atttgctggt
     3361 taccaataac ccgattcata agaaggaaca gacaccagtc tattatttaa aaaatgactt
     3421 ggatatggag gatttggtag cgattcgcca gttattattc acttaaaagg cttggttaat
     3481 ccaggtcttt tttgtgaaat tcacacaatc tcctcacatt tttttaaaaa ttaaaaaaag
     3541 ttgataaaca agaaagcgct ttattttgta tactagtaag tgtaaagagg aaacacctca
     3601 agatctttat caggaggaca gtacatgtca caagaaaaat acatcatggc cattgaccag
     3661 ggaactacaa gttctcgtgc catcattttc aacaaaaaag gggaaaaggt tagctcgagt
     3721 caaaaagagt ttacccagat tttccctcaa gcaggttggg ttgagcacaa tgccaatgaa
     3781 atttggaact ctgttcagtc agttattgcg ggtgctttca tcgaaagtgg tgtcaagcca
     3841 aatcaaatcg aggcaatcgg gattaccaac caacgtgaaa caacggttgt ctgggataag
     3901 aaaacaggac ttcctatcta caatgctatc gtttggcagt cacgccagac agcacctttg
     3961 gctgagcaac taaaaagcca aggttatgtg gaaaaattcc atgaaaagac tggtttgatt
     4021 attgatgctt acttctctgc taccaaggtt cgttggattt tggatcatgt agaaggtgct
     4081 caagagcgag cagaaaaagg ggaattgctc tttggtacta tcgatacttg gttggtttgg
     4141 aaattgactg acggtgcggc tcacgtgact gactactcaa atgcagctcg taccatgctt
     4201 tataacatta aagaactcaa atgggatgat gagattttgg aaatccttaa cattccgaag
     4261 gctatacttc cagaagttcg ttctaactcc gaaatctacg gcaagacagc tccattccat
     4321 ttctacggtg gagaggtgcc aatctcaggt atggctgggg accaacaagc agccctcttt
     4381 ggacagttgg cttttgagcc aggtatggtt aagaatactt atggaacagg ctctttcatc
     4441 atcatgaata ctggggaaga gatgcagttg tctgaaaaca acctcttgac aaccattggt
     4501 tacggaatca acggtaaggt ttattatgcc ttggaaggtt ctatcttcat cgcaggaagt
     4561 gctattcagt ggcttcgtga cggtcttcgc atggttgaaa attcaccaga atctgaaaaa
     4621 tacgctcgtg attctcacaa caacgatgaa gtttatgtcg ttccagcctt tacaggtcta
     4681 ggcgctccat actggaacca aaatgctcgt ggttccgtct ttggtttgac tcgtggaaca
     4741 agcaaagaag actttatcaa ggcgactttg caatctattg cttatcaagt gcgtgatatc
     4801 atcgacacca tgcaagtgga tactcagacc gccattcaag tactgaaggt ggatggtggt
     4861 gcagccatga acaacttcct catgcagttc caggcggata ttttaggcat tgacattgca
     4921 cgtgctaaaa acctggaaac aacagctcta ggagcggcct tcctagcagg tttgtcagta
     4981 gggtactgga aagacttgga cgagttgaaa ctcttgaacg agacaggaga actctttgag
     5041 ccatctatga acgaatctcg caaggaacaa ctctacaagg gctggaagaa ggctgtgaaa
     5101 gcaactcaag tctttgcgga agtagacgac taatactggc agaataaagc gatttattta
     5161 gaaagtgtgt aaatatggaa ttttcaaaga aaacacgtga attgtcaatt aaaaaaatgc
     5221 aggaacgtac cctggacctc ttgattatcg gtggaggaat cacaggagct ggtgtagcct
     5281 tgcaggcggc agctagcggt cttgagactg gtttgattga aatgcaagac tttgcagaag
     5341 gaacatctag tcgttcaaca aaattggttc acggaggact tcgttacctc aaacaattcg
     5401 acgtagaagt ggtctcagat acggtttctg aacgtgcagt ggttcaacaa atcgctccac
     5461 acattccaaa atcagatcca atgctcttac cagtttacga tgaagatgga gcaaccttta
     5521 gcctcttccg tcttaaagta gccatggact tgtacgacct cttggcaggt gttagcaaca
     5581 caccaactgc gaacaaggtt ttgagcaagg atcaagtctt ggaacgccag ccaaacttga
     5641 agaaggaagg cttggtagga ggtggagtgt atcttgactt ccgtaacaac gatgcgcgtc
     5701 tcgtgattga aaacatcaaa cgtgccaacc aagacggtgc cctcattgcc aaccacgtga
     5761 aggcagaagg cttccttttt gacgaaagtg gcaagattac aggtgttgta gctcgtgatc
     5821 tcttgacaga ccaagtattt gaaatcaagg cccgtctggt tattaataca acaggtcctt
     5881 ggagtgataa agtacgtaat ttgtctaata agggaacgca attctcacaa atgcgcccaa
     5941 ctaagggagt tcacttggta gtggattcaa gcaaaatcaa ggtttcacag ccagtttact
     6001 ttgacacagg tttgggtgac ggtcgtatgg tctttgttct cccacgtgaa aacaagactt
     6061 actttggtac aactgataca gactacacag gtgatttgga gcatccaaaa gtaactcaag
     6121 aagatgtaga ttatctactt ggcattgtca acaaccgctt tccagaatcc aacatcacca
     6181 ttgatgatat cgaaagcagc tgggcaggtc ttcgtccatt gattgcaggg aacagtgcct
     6241 ctgactataa tggtggaaat aacggtacca tcagtgatga aagctttgac aacttgattg
     6301 cgactgttga atcttatctc tccaaagaaa aaacacgtga agatgttgag tctgctgtca
     6361 gcaagcttga aagtagcaca tctgagaaat atttggatcc atctgcagtt tctcgtgggt
     6421 ctagcttgga ccgtgatgac aatggtcttt tgactcttgc tggcggtaaa atcacagact
     6481 accgtaagat ggctgaagga gctatggagc gcgtggttga catcctcaaa gcagaatttg
     6541 accgtagctt taaattaatc aattctaaaa cttaccctgt ttcaggtgga gaattgaacc
     6601 cagcaaatgt ggattcagaa atcgaagcct ttgcgcaact tggagtatca cgtggtttgg
     6661 atagcaagga agctcactat ctggcaaatc tttacggttc aaatgcaccg aaagtctttg
     6721 cacttgctca cagcttggaa caagcgccag gactcagctt ggcagatacc ttgtcccttc
     6781 actatgcaat gcgcaacgag ttggctctta gcccagttga cttccttctt cgtcgtacca
     6841 accatatgct ctttatgcgt gatagcttgg atagcatcgt tgagccagtt ttggatgaaa
     6901 tgggacgatt ctatgactgg acagaagaag aaaaagcaac ttaccgtgct gatgtcgaag
     6961 cagctctcgc taacaacgat ttagcagaat taaaaaatta agaaaaaata aaagaggtgg
     7021 agggcagcat tccttgtcgc ccgtcccttc tttttaatgg agacagaaag atgatgaatg
     7081 aattatttgg agaatttcta gggactttaa tcctgattct tctaggaaat ggtgttgttg
     7141 caggtgtggt tcttcctaaa accaagagca atagctcagg ttggattgtg attactatgg
     7201 gttgggggat tgcagttgcg gttgcagtct ttgtatctgg caagctcagt ccagctcatt
     7261 taaatccagc tgtgacagtt ggtgtagctt taaaaggtga cttgccttgg gcttccgttt
     7321 tgccttatat cttagcccag ttcgcagggg ccatgctggg tcagattttg gtttggttgc
     7381 aattcaaacc tcactatgag gcagaagaaa atgcaggcaa tatcctggca accttcagta
     7441 ctggaccagc catcaaggat actgtatcaa acttgattag cgaaatcctt ggaacctttg
     7501 ttttggtgtt gacaatcttt gctttgggtc tttacgattt tcaggcaggt atcggaacct
     7561 ttgcagtggg aactttgatt gtcggtatcg gtctatcact aggtgggaca acaggttatg
     7621 ccttgaaccc agctcgtgac cttggacctc gtatcatgca cagcatcttg ccaattccaa
     7681 acaagggaga cggagactgg tcttacgctt ggattcctgt tgtaggccct gttatcggag
     7741 cagccttggc agtgcttgta ttctcacttt tctagtttat actcttcgaa aatcaaattc
     7801 aaaccacgtc agcgtcgcct taccgtactc aagtacagct tgcggctagc ttcctagttt
     7861 gctctttgat tttcattgag tattagaaaa caattatttt gatagagctt gggcaagagc
     7921 ccaatttcag caaaaaatga agtaaatctt ctcataataa aacgcatcat atcaagcacg
     7981 aaaattccac gaggtcaact acagtcagaa agctgaacaa caagccaaaa cgcccaaaaa
     8041 aggcggcaaa aagcaagcac ctgcaagcaa cgtgccgaaa tggtcaaatc ctgattatgt
     8101 caacgaatta gacccaaaaa tcgttgatat gctagtagaa tttcacaagt cacaaggcac
     8161 tttggaaact cccgaggcgc aagcagaaat cgcccaaaaa cgtgaagaaa tcgagcaaag
     8221 gagagctgag cttgaggata aaaaacaaga gcttttgaac cgcttgaaca aatagagttt
     8281 cgcaagtatt atgcttacaa attacttgag caattaacta aaatataaac cctgcctaga
     8341 tataaaggca gggtttatat tttagaaatt cacgtaggtt gttacgg
//
LOCUS       DAVMHG010000093         8285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMHG010000093 DAVMHG010000000
VERSION     DAVMHG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.093"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            130..429
                     /locus_tag="VYR08_001542"
                     /pseudo
     CDS             130..429
                     /locus_tag="VYR08_001542"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(707..1477)
                     /locus_tag="VYR08_001543"
     CDS             complement(707..1477)
                     /locus_tag="VYR08_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV3467050.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(1492..2340)
                     /locus_tag="VYR08_001544"
     CDS             complement(1492..2340)
                     /locus_tag="VYR08_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV3467051.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGTPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(2337..3812)
                     /locus_tag="VYR08_001545"
     CDS             complement(2337..3812)
                     /locus_tag="VYR08_001545"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV3467052.1"
                     /translation="MVQTKQPNIILIVVDQMRADVLSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(4135..5490)
                     /locus_tag="VYR08_001546"
     CDS             complement(4135..5490)
                     /locus_tag="VYR08_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3467053.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(5500..5817)
                     /locus_tag="VYR08_001547"
     CDS             complement(5500..5817)
                     /locus_tag="VYR08_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3467054.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5834..6151)
                     /locus_tag="VYR08_001548"
     CDS             complement(5834..6151)
                     /locus_tag="VYR08_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3467055.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(6166..7062)
                     /locus_tag="VYR08_001549"
     CDS             complement(6166..7062)
                     /locus_tag="VYR08_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV3467056.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(7059..8255)
                     /locus_tag="VYR08_001550"
     CDS             complement(7059..8255)
                     /locus_tag="VYR08_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3467057.1"
                     /translation="MRDGITLTIPLLLIGSLLMVIASFPIPGWEKYLGDIGVADYLWK
                     GVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGIVSLSSFITVTPFIRGEAGAGMPTA
                     FMASKGLFVAMILGLINGYIYQWFINHNIQIKMPDGVPPAVSKSFSAIIPGAVTIVGW
                     LIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVIGLLILIFLNSSFWFVGLHGGNVV
                     NAVMKPLWLANLDANKVAYQTGETLPNIFTSVFMDNFVFIGGGGATIGLVLALGYLAH
                     KKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVLNILLLVPFILAPMFNLLVAWGAM
                     ASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQIVLIVLDVLLYLPFVIAIEKRFK
                     LLEDCE"
BASE COUNT         2854 a         1797 c         1196 g         2438 t
ORIGIN      
        1 attttcgtac aggtgcttca atagtttcta tagtaaaatg aaccaaaaat agtacacgtt
       61 gcggtataat gttcttgtgg catattcatt agattttcgt aaaaagttct ctcctatttg
      121 tgagcgaaca ggtatagtca attgattgtc agtttgaaga ccaagagtcg taaggcaaag
      181 gatatggcag aatccatcca agggtggctg gcacaatttc tagtgaacct ctttaaatct
      241 attacgtttg actgtggaaa agagttttca aaatggaaag atatttccaa tcatcacgat
      301 agtgagagct tttttgcgaa tctaggatgt ccaagacagc ggtgtctcaa cgaacattca
      361 aatagacttt taaggtgtca tgacttgcca aagcagacag atttcaacga agtcagtcaa
      421 gaattttagg ctgggtaaaa actcaatttc aggagaaaat gaaataaata ttcccacaat
      481 aaaacgcata gtatcaaagt ttttccaaca cctgatatta tacatttttt aattttaaag
      541 actttttccc agcctcaaga aatcacatcc cacctaaatc ctttggatac caaatacctc
      601 atcaggtatt ttctgagttg gtaagactgt ctagcttaat ttgacaaatg gagggcgtta
      661 aatccagttt ttacaggaag tctatttaaa tattttcaca ctgaccttac aaaatatgaa
      721 ataaattata taaagtcatt cccatgacaa agaataacat ccctttaaaa cagtatctta
      781 cccaattttc aggtaagact tttgatacaa ccgtccccaa tacccctccg catatagaag
      841 caagaaaaat aactggaaca ggtgctaaat gatactgatt taaacctgta accacaacaa
      901 ttgtagccaa atgagacaac tgggaaaaga aaataattgc aagagaatac atcgttgctt
      961 cttttatcga aatagaaaaa aacaccatca ataaagatac attgagcgga ccaccaccaa
     1021 tccctaaaaa actagaaatc aagcccaata gaagaccaca aatcatatag aatcctatct
     1081 tatcaaattg ataagttttt ttaaaatctg ttaaaacaaa tgatacaagt agcatcacaa
     1141 atagtaaaat catttgaaca atggatacta gccgtactga taactgagat aacaaaactt
     1201 gaaaaataag atgacctaga aatcctccta aaacagaaga aaaggatacc gtaaaaatca
     1261 atctccattt tatctcttta ctttgtgaaa atcttttgat tgttgaaata atcgccatta
     1321 taaaaacaga gaaactagaa taaaaagcaa tatcactgac cgaatggtat ccaaaactat
     1381 ccataagtgg cttaattata attccaccac ccatgcctga tatagaaccc aatccactag
     1441 ctattataat gattagaaaa taaataatct caaccacttt ttaagtccat cctattcttt
     1501 caaacatcta aaacctagat ggctactagt ggaagtagaa atacatccat ttctagcagc
     1561 tactcggtat cgattacaat aagaacaatg acataaaaag gaacctcctc tgatagcata
     1621 ctcctcatct tgtatcccat attttggtaa ctcatagtca tcttcattaa aactagctaa
     1681 taaggtatat ctcggattgc gacaccattc ccaaacattc ccaatcattt gatatagacc
     1741 aaaatcatta ggtaaaaatt catagacagg agccgttcct ataaatccat ctaaggcagt
     1801 attttcataa ggaaatcttc cttgccaagt attggcatga taatatccac cttctaaaag
     1861 agtatctccc caaggatatt cagattgtct tcctcctcta gcagcatatt cccactgtgc
     1921 ttcagtcggt aaagacattc cgctccaatt acagaaagcc aatgcatctt ctaaagcaac
     1981 atgaactact ggatggtctt ctaaacccac tagattacta ttctcaccgt agggatgttt
     2041 ccagcaagcg ttcggaactt gtaaccacca cggagtccca gcaggatgag gatatccctc
     2101 cctttctgcc tctggaacga acaatataaa aacaaaagac cactcttgtc gctcagctag
     2161 tgttttatag cctgtttctt tcacaaattg agcaaattct tgattcgtaa ctggagtatc
     2221 cgcaatggaa aaatccttta cactaacagc gacttgagga ccttcaaaat catctaaaaa
     2281 gccctcttca gaatttgttc ccatcataaa tgtcccaccc ttgatttttt tcattttcat
     2341 gattgactat cccccgtttt cgttagagtt ggcgttattt tagaaagttc aacaggaact
     2401 aactcttcat ccacaacaaa accttcttct ctatatctaa gaaagtctac taattttttc
     2461 ttcatctgac gaacaatagg ttggtatttt tcacttggat acaaatcatt catttcatgc
     2521 ggatcttttt tcatatcaaa taactgataa tgatttaaaa caggaaacca gataaatttc
     2581 cactggtctg tcaagatata ttgactagaa tcttttccta aagcatgttc accatgaaat
     2641 tctgttcgcc acccctcgta ctgtccaaat aataaattct taacactccg tccatctagt
     2701 tcatctgtag tagttcctcc tgccaaatcc acgagtgaag gaaaaatatc ttgaattttc
     2761 actaattgct tgattgttcc cctattacca gctatcaatc cagccggatc gtaaataaac
     2821 gaaggaatgt ggatacttcc ctgataagga tatccctttc taaacagata atgttcacca
     2881 agctgatctc catgatccga aacaaaccag ataatcgtat ctttatcatg acgaaattct
     2941 tttagggcag tcaaaaagcg actaatttga tggtctatat gagtaatcaa tccaaaataa
     3001 gcagcaacca ttcttctttg atcatcttcc tttaatttcc ctcttaaagc ataaatagac
     3061 ggaatttgct tttctaatac ttcccaattc ccgatatgca aatcgagaaa ttgaggcaag
     3121 cgttccatat acatatcaaa atagtatttt gggggattta atggagcatg tggtttttca
     3181 aaagacattt tgagaaaaaa aggaaccgtt ggatctcttc tttgtaaaaa tgaaatggac
     3241 tcactcacaa cccagtttgt tggatgtaac ttctcatctt tatcccaagg tctagcttcc
     3301 caagaattac aatccattcc gtcatcaatc aaatcaacat cataaccaac tttacccttt
     3361 aaaaaggcta gatagtcact agcataatca aattgacttc cataagcctt atcatacttt
     3421 ctatcaacat gtagataccc atcgtgaagt aagacatgat caaacccaag tctctgtctg
     3481 gaaggaaaaa catgcatctt accaatacac tctgtttgat aacccatatc tttaaagact
     3541 tttggaagag tattagtaaa attccaaggc acctcatctt gataaccaac tcgtccactt
     3601 ttatcttggt ctaagccagt caataaagct gccctagcag gaacacaaga aggaactgga
     3661 gaataggcat tttcaaaatt ataacctaca ctagccatca tatctaatgt tggagtgcta
     3721 actagcttat ccttactatt caaggataag acgtctgctc tcatttgatc tacaacaatc
     3781 aaaataatat ttggctgttt tgtctgaacc ataaaatctc ctttctaata tggcaaaaga
     3841 ggcacaagaa gatatctacc tttactgcac ccctttctat atcaatctct ctatataaaa
     3901 caataacatt cttgttatgt tttatagaaa aatgaactaa aatatgacta aatcgattag
     3961 gaaattcaaa tcattttcta gtactgtttt agaagttaca gtgtactatt ccaacttcaa
     4021 taaattataa acctttgtct aataacaatt ttagtggaga taagaaatcc tacacctaac
     4081 tcatcttaca cgtaatctat ttctatttta tcacaaaaaa cgcaagtaag accattaact
     4141 caattcagtt ttatctgcca ttttcacaaa tgggaaataa gtcaagacac taataatcaa
     4201 acaaacaact gataagatga tggcacgcca atcaaatgct gtagagaaga aaccatataa
     4261 aattggaggc attacccaag taacgttttg tgtaacaggt gaaacaagac cccagcttgt
     4321 tgcccagtaa gctaccgttg ccatgaaaac cgggctaagt acaaatggta taaatagcaa
     4381 aggattcaag acaactggta aaccataatt gaccggctca ccaatattaa acagaactgg
     4441 tgctagacca agtttagcaa cttttcgata atgactgttt cttgaaaaaa ttaaaatagc
     4501 aagtactaat cctaatcctc caaaccagac aaacgcccca aaagacccac ttgtccatat
     4561 ataaggaatc ggttcacctt tttggaaagc atccagattc gctaacatag caactccaaa
     4621 cagcccttcc atgatgggag ccaatacatt tcctccatgg agaccaaaaa accagaataa
     4681 cttattcaaa aagatcatca gaataactgc aaagaaactt tgagacaaac ctagtaatgg
     4741 cgtttgtaac accttgtaaa cccaatcaat caataagtca ttgctaagta aatggaaaac
     4801 ataagtcaag atggctacta tatacatcgc cataaatcct ggaatgatag aagtgaacgg
     4861 cttagcaatc gcagggggaa ctgaatctgg taacttgatt acccagttct ttttcattac
     4921 tttacagaaa ataatagagg ctaaaaatcc aatcatcatg gctgtaaagt agcctctggc
     4981 attaatatgg tttcctggaa tcacattccc aatagttacc atcagatttt taccatcaaa
     5041 tgctagatta tcaattcctt gtaagatttg atctaatttc acatctccta catttgccaa
     5101 agggaaactc tttgtaactg tacttccaat cgaaatgaca aacgaagcaa gcgataccaa
     5161 accagcagaa actgtatcaa ccttataaat cttagcgata ttcactccca agcaatagat
     5221 gaacaataag gaaacaattg gtatacttcc cttgaatacc aaattattga tgtcaacaag
     5281 ccactgaaag gttttcgtaa tactttctag gtgaaattgt tgtggtaaat ccactagaaa
     5341 agcatttaat aacaaagcaa tggaacctgt cataataaca ggcatagtcc ccacaaatga
     5401 atcacgtaaa gcaactaaaa acttctgatt cccaactttc attgcaattg gagaaagttt
     5461 ttcttgcatt ttatccaaca ttgtattcat agcaaattcc taattttttt caaatcgttc
     5521 gtatacttct accacttctt tcgccaagtc aacaaaggtc agactcgtca ttaaatgatc
     5581 ttgcgcgtgt accatcaaga ggctaattga tgtttcttcc ccctgtgcct cttttgtcaa
     5641 catttctgtt tgagccttat gcgcctgtaa taaggcagca ttcgcatcag ctaatcttcg
     5701 attggcttta gagaaatccc ctttttttgc tgcctgaata gcctccatag cattactttt
     5761 ggcttcccca ccatacataa ttagccccat tgcagattct aaattacttt cattcatagc
     5821 attctcctca atcttagtct aataaacttt cagctaaatc taagacttta gaaccattca
     5881 tcattccata atctgtcatc ggaataaccg ccacaggaat ctgtctatct tttagttttt
     5941 cttgaaagtc ccctagtaaa tagcgaactt gagggcctaa aagcaacaca tttacttctt
     6001 ttgttgctac aatttcttct gcttcaggag ctggaactgc aaaaatttct gcatccaacc
     6061 ccttatcttc tgctgccttt tgcatctttg tcactagcag acttgtactc atacctgctg
     6121 cacatgctaa cataattgtt actttagcca tagcttactc ctttatcacc tatttactga
     6181 cctagccaag cgagatactg tatgtctcac tcctctaatt gccttgctca atgcataaat
     6241 attatcgact gttgccagtc ccccatatcc tgcgtcacct atatgttgaa tatcaactcc
     6301 acaaatttta tttctaagtg caatttcctt aatagtatct gtatcagatg tttcttggct
     6361 agtaccaata gcactcaata caagaccacc cttactatga acgagatcaa cgacttcacg
     6421 caactcttgg tcatgaaagg ctggaacggt tccaactgct ggaacaagta tcacatccgc
     6481 acctgcttcc aacaattgct ctgcgacaga aagctctgcc acaggctcat tcactcctgc
     6541 cccgtgcatc ttacctgcaa taatcaaacc agaaaagttt tccttagcag tttgaacagc
     6601 cttaatgatt tctcgattgc taactcctgt tccaggattt ccagtcaaac agacaaagtc
     6661 aaaacctaac tcctctattc gcttcaatgt ttcaacacta gcaacacgac ctgcaacaat
     6721 ttcctgtgtt tcctctagca tctttgcaga tgggtcaatc ggttccaaat ttacaccaat
     6781 tggacaagca acaagctggt gtaagcgatg aataacatca tcacctgaac tatccaaagc
     6841 ataaatttta ggctcaaaaa catcaacaca attcaataaa atcatatcag ctccaaaagc
     6901 tctagcaatt tcagaattag ttatatcccc aacaaatgtc tcacgagtaa ctacattttc
     6961 agatagaaca actctacctt cacttgctaa aatactttgt tttaattctt cagcattcat
     7021 ctgtaatatc tcagatggat ttgcactaat taaacgtttc attcgcaatc ctccaataac
     7081 ttaaaccgtt tttcaatagc tattacaaat ggtaaataaa gtaagacatc caaaacaatc
     7141 aatacaattt gtaataatga accagaaata cttcctgtag caagtaaacc acttataaca
     7201 ggtggcatag tccaacctgg atctgtataa gttagcggaa ccaaacctga tgccattgct
     7261 ccccacgcta ctagtaagtt aaacattgga gctaagataa atggaactag taacaaaatg
     7321 ttcaaaacaa taggaacgcc aaacattgct ggttcattta tattaaataa tccaggtata
     7381 acagttatag gtgccaaagt ttttagttgt ttactagctt tcttcttatg tgctaaatat
     7441 cctaacgcaa gaactaaacc tatggttgca ccaccgccac ctatgaatac aaaattatcc
     7501 ataaatacgc tagtaaaaat atttggaaga gtttctccag tttgataagc tactttattt
     7561 gcatctagat tagctaacca taacggtttc ataactgcat taacaacatt tcctccgtgt
     7621 aatcctacaa accaaaaact actattaagg aaaattaaga taagaaggcc aataacatta
     7681 tttcctaaaa gtccaagtgg acctcccaaa gctacttgag caatttcatg aagattaggt
     7741 aaacttaatt tatctaaagt tgcataaaca atcaaccaac caacaatagt cactgcacca
     7801 ggaataatgg cactaaagct tttagatact gctggtggaa caccatccgg catttttatc
     7861 tgtatattat gatttataaa ccattggtag atatagccat taatcaatcc taaaatcata
     7921 gcaacaaata aaccttttga tgccatgaaa gcggtaggca ttcctgctcc agcctctcct
     7981 ctaatgaaag gcgtaactgt aataaaagat gataatgaca caataccagc aggaatacca
     8041 tcaactttgt attgtctagc cataaagtaa gcgataccaa aactagctac aagaccaagt
     8101 aatccaaaac tactatcaac acccttccat aaatagtcag caacaccaat atcacccaga
     8161 tatttttccc atcctggaat aggaaaactt gcaataacca ttagtaatga acctatcaac
     8221 aataaaggta tagttagagt aataccgtct cgaatagaaa ccaacgcttt attattaccc
     8281 aatct
//
LOCUS       DAVMHG010000094         8238 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMHG010000094 DAVMHG010000000
VERSION     DAVMHG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.094"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..840
                     /gene="sufC"
                     /locus_tag="VYR08_001551"
     CDS             70..840
                     /gene="sufC"
                     /locus_tag="VYR08_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV3467058.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            868..2130
                     /gene="sufD"
                     /locus_tag="VYR08_001552"
     CDS             868..2130
                     /gene="sufD"
                     /locus_tag="VYR08_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV3467059.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGDDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            2141..3367
                     /locus_tag="VYR08_001553"
     CDS             2141..3367
                     /locus_tag="VYR08_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV3467060.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            3354..3794
                     /locus_tag="VYR08_001554"
     CDS             3354..3794
                     /locus_tag="VYR08_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV3467061.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            3848..5260
                     /gene="sufB"
                     /locus_tag="VYR08_001555"
     CDS             3848..5260
                     /gene="sufB"
                     /locus_tag="VYR08_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV3467062.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(5491..6732)
                     /locus_tag="VYR08_001556"
     CDS             complement(5491..6732)
                     /locus_tag="VYR08_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV3467063.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            6835..7944
                     /locus_tag="VYR08_001557"
     CDS             6835..7944
                     /locus_tag="VYR08_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV3467064.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         2445 a         1504 c         1788 g         2501 t
ORIGIN      
        1 ctatttttgg ggaaatcaga cgtttttcta gtaagtactg taaaagtttt gaaaaagaaa
       61 ggaactatca tgtcagtatt agagatcaaa gatcttcatg ttgagattga aggaaaagaa
      121 attttaaaag gggttaacct gaccctgaaa acaggagaaa ttgccgctat catgggacca
      181 aatggtacag gtaaatcgac tctttctgcc gctatcatgg gaaatccaaa ctatgaagta
      241 actaaaggtg aagttttgtt tgatggcgta aacatccttg agttggaagt ggatgagcgt
      301 gcgcgtatgg gacttttcct tgctatgcaa tacccatcag aaatccctgg aattaccaat
      361 gctgagtttc ttcgtgccgc tatgaatgcg ggtaaagaag atgatgagaa gatttcagtt
      421 cgtgagttta ttactaagct agatgaaaaa atggaattgc tcaacatgaa agaagaaatg
      481 gcagagcgtt acctcaacga aggcttctct ggtggtgaga aaaaacgcaa tgaaattctt
      541 caacttttga tgttggagcc aacatttgct cttttggatg agattgactc aggtcttgat
      601 attgacgctc ttaaagttgt gtctaaaggt gtcaatgcca tgcgtggtga aggttttggt
      661 gctatgatca ttactcacta ccaacgtctt ttgaactata tcacacctga tgtggtacac
      721 gtgatgatgg aaggtcgtgt tgtcctttct ggtggtccag aattggctgc gcgtttggaa
      781 cgtgaaggat acgcaaaact agctgaagaa cttggctacg actacaagga agaattgtaa
      841 ttccctcgta tcttttagga gaagtaaatg actagagaaa atattaaact tttttcagaa
      901 atgcacgctg aaccaagctg gttggctgat ctccgtcaaa aagcttttga caagattgag
      961 actttggaat taccagttat tgagcgtgtc aaattccacc gttggaatct gggtgatgga
     1021 acgattacag aaaatgagcc atcagcaaat gttccagatt tcacagcttt agatcatcac
     1081 ttgaagttgg tgcaagtagg aactcaaact gttttcgaac aaactccagt tgagttagct
     1141 gaacagggtg ttgtcttcac agactttcac tcagctttag aagaaattcc agagctgatc
     1201 gaagaattct tcatgtcatc tgttaagtat gatgatgaca agttggcggc ttaccacaca
     1261 gcttacttta acagtggtgc tgtactctat attccagata acgtagaaat tacagagcca
     1321 attgaaggaa ttttctacca agatagcgat agcaatgtgc cgtttaacaa gcatattatg
     1381 attatcgctg gtaaaaattc taagattagt tatctggagc gtttagagtc acgcggtgaa
     1441 ggaagtgaca aagcaactgc caatatcaca gtggaagtga ttgcacgttc tggtgcgcaa
     1501 gtcaagtttg ctgctatcga ccgtctaggt gaaaacgtca ctgcctacat tagccgtcgt
     1561 ggtaaattag gcgacgatgc aagtattgac tgggctatcg gtgtcatgaa cgaaggaaat
     1621 gtcgttgctg attttgatag tgacttgatt ggtaatggta gccatgctga cctgaaggtt
     1681 gtagctcttt caagtggtcg tcaggtacaa gggattgata ctcgtgtaac taactatggc
     1741 tgcaactcta tcggaaatat cctacaacat ggggttatcc ttgaaaaagc aactttgact
     1801 ttcaatggta tcggccacat catcaagggt gctaagggag cagatgcgca acaagagagc
     1861 cgtgttctta tgctttcaga ccaagcgcgt tcagatgcta acccaattct tttgattgat
     1921 gaaaatgacg taactgcagg acatgcagcc tctattggtc aggtagatcc agaagacatg
     1981 tattacctca tgagtcgtgg cttggataag gcaactgcag agcgtttggt tgttcgtggt
     2041 ttccttggat ctgttatcgt ggagattcca gtcaaggaag ttcgtgatga aatgattgca
     2101 actatcgaag agaaattgtc aaaacgctaa ggggaagcct atgttagatg tagaagcgat
     2161 tcgcaaggat tttccaattt tagatcagat tgtcaatgat gaacctctgg tctatctgga
     2221 caatgctgcg acgacacaaa aaccactagt agttctgaaa gctattaaca gctactatga
     2281 gcaggacaat gccaatgttc accgtggtgt ccatacctta gcggaacgag cgacagcttc
     2341 ttatgaagct gctcgtgaaa ccattcgtaa gtttattaat gcaggctcta caaaggaagt
     2401 tctctttacc agaggaacga caaccagcct taactgggtg gcacgctttg ctgaggaaat
     2461 tctcgctgag ggagaccagg tcttgatttc agtaatggaa caccattcta atatcattcc
     2521 atggcaggaa gcttgtcgaa agactggagc agagcttgtc tatgtctatc ttaaagacgg
     2581 tgccttggat atggaggatt tgcgagctaa attgactgat aaggttaaat ttgtttccct
     2641 agctcatgcc tccaatgttc ttggtgtggt caatccgatc aaggaaatca ctcaattagc
     2701 ccaccaagtt ggggcaatta tggtagtgga tggtgctcaa tctacacctc atatgaagat
     2761 tgatgtccag gacttggatc tggacttttt cgccttttcg ggtcacaaga tggctggtcc
     2821 gactggtatc ggtgtccttt acggcaaaga aaagtatctt gagcaaatgt ctccagtaga
     2881 atttggcggc gagatgattg attttgtcta cgagcaattt gctagttgga aggaattgcc
     2941 ttggaaattt gaggctggaa cgccaaatat ggcaggagct attggacttg cgactgcagt
     3001 tgattatctg gaaaagattg gtatggatgc cgttgaagct catgaacagg aattgattgc
     3061 gtacgtctat ccaaaactgc aggcaattga gggattgacc atttacggtt ctcaggattt
     3121 ggctcaacgt tcgggtgtta ttgcctttaa cctaggtgat ctccatcctc acgatcttgc
     3181 gacggctctg gattatgaag gagtggctgt tcgtgctggt caccattgtg cgcaaccctt
     3241 gcttcagtat ttggaagtcc cagcaacagc tcgtgcaagt ttttatatct acaataccaa
     3301 ggcagattgc gacaaactag tcgatgccct acaaaagaca aaggagtttt tcaatggcac
     3361 tttctaaact agatagcctt tatatggcag tggtagcaga ccattcgaaa aatccacatc
     3421 accaagggaa gttagaagat gctgagcaaa ccagtctcaa caatccgact tgtggggatg
     3481 tcatcaacct ctctgtcaag tttgatgcag aggaccgttt ggaagatatt gcttttctaa
     3541 attcaggatg cacgatttca actgcttctg ctagtatgat gacagatgcc gttttaggaa
     3601 aaaccaaaca agaaatttta gaactggcga ctattttttc tgaaatggtt caggggcaaa
     3661 aagatgagcg tcaaggccaa cttggagacg cggcattctt gtcaggtgtt gccaaattcc
     3721 ctcaaagaat caagtgtgca accctagctt ggaatgccct taagaaaaca attgaaaatc
     3781 aagaaaaaca gtaagacaag tttcttttgt cttatgaatt attagaaatg aagaaagaaa
     3841 ggatactatg gctgaagaaa gagtagaacc aaaaccaatt gaccttggtg aatataaatt
     3901 tggtttccat gacgatgtag agcctgtctt atcgacagga aaaggactca acgaaggtgt
     3961 tattcgtgaa ttatctgctg ctaagggtga gcctgagtgg atgttggagt tccgtttgaa
     4021 gtcttatgaa accttcaaaa aaatgcccat gcaaacttgg ggagcagact tgtcagagat
     4081 tgactttgat gacttaatct actaccaaaa accatctgac aaaccagccc gttcttggga
     4141 tgatgtacct gaaaagatta aagaaacctt tgaacgtatc gggattccag aagctgaacg
     4201 tgcttattta gcaggggctt ctgcccagta cgagtcagaa gtggtttacc acaacatgaa
     4261 ggaagagttc caaaaattag gtattatctt tacagataca gattccgcac tcaaggaata
     4321 cccagactta tttaaacaat actttgcgaa gttggtaccg ccgacagata acaagttggc
     4381 agccctcaac tcagcagtat ggtcgggtgg aacttttatc tacgtgccaa aaggtgtcaa
     4441 ggtagatatt ccacttcaaa cttatttccg tatcaataac gaaaatatag gtcagttcga
     4501 acgtaccttg attatcgttg atgagggagc aagcgtccac tacgtagaag gatgtacagc
     4561 accaacatat tcaagcaata gcttacacgc tgccattgta gaaatttttg ctttggacgg
     4621 agcttatatg cgttatacaa ctatccaaaa ctggtctgat aacgtctata acttggtaac
     4681 aaagcgtgct aaggctcaaa aggatgccac tgttgagtgg attgatggaa acttgggtgc
     4741 caaaacgact atgaaatatc catctgttta ccttgatgga gaaggagcgc gtggtaccat
     4801 gctctctatc gcctttgcta atgcagggca acaccaagac acgggtgcta agatgattca
     4861 caatgctcca cataccagct cgtctattgt gtctaaatcc atcgctaaag gtggaggaaa
     4921 ggttgactac cgtggacaag tcacctttaa caagaactct aagaaatctg tttcccacat
     4981 tgaatgtgat accattatca tggatgactt gtcagcatca gatactattc catttaatga
     5041 aattcacaac tcgcaagtgg ctttggaaca cgaagccaaa gtatctaaga tttcagaaga
     5101 gcaattgtat tatctcatga gccgtggatt gtcagaatct gaggcaactg aaatgattgt
     5161 catgggattt gtagaaccct ttacaaaaga acttccaatg gaatacgcag ttgagctgaa
     5221 ccgcttgatt agctatgaaa tggagggatc agttggataa aatttgattt tatactcttc
     5281 gaaaatctct tcaaaccacg tcagcatcgc cttaccgtat gtatggttac tgacttcgtc
     5341 agtttcatct acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagttta
     5401 ttctttgatt ttgagtatta gatttactca aaatcaagga ttttgaagat gaacttgtat
     5461 caaaaaatcg cggtttaaaa tcgcgatttt ttataatttc tcgttaacaa agcggacaaa
     5521 ctgattccac caaactttta agaagaaggc tttttcaatt ttcttgtctg ctaccatttc
     5581 gaaactaggg cgctctgtgg tgatgtaacc ttgaccaatc aagttcttgt cttcataagt
     5641 caaatggcca accactgttc cagcttcaag tggtgctggg attgctttgg aatcaggtgt
     5701 gaattgaaca gattgggaag attgattccc aacacgttct attagataga tatcctctgg
     5761 agccactgca gttactgtat cttcttttcc atcttgtaca ggggctttgc tatcttgata
     5821 ggcatcgcct tgttgaacga ttttgcgaag tgtaaatgta gaagaaatat aatccattag
     5881 ggaagatgta gctgtaaatc gagcgtaagg attattgtct tgatgatctg catttaaaac
     5941 aactgtgatg actctcatgc ctttttcgac agtagtacca acaaaagact ctccagcctt
     6001 atctgttgtt cctgttttta gcccatcaaa accaccacgg taagcaggca taccttctaa
     6061 catgtagttg gttgaagtga ttgtcatccc agcaaaagta gaagaaggtt ttttggtgat
     6121 ttctaagact tgtgggtatt ttttgatgag gttgcgagca acgatagcga catcataagc
     6181 actaagctta ttttcctcat cttttttaga acctgggtaa atgttatccc ctagagtttc
     6241 attgttaaga cctgtcgtat tgacaacagt ggcatcctga attccccatt ccaagagttt
     6301 tgcccgcatc atatcgacga aatctttttc tgagccagca attttctcag ctagggcaat
     6361 agcggcgctg ttggcactag ataccagagt tgcttcaagc aactcttcga cagtataatt
     6421 acgggcctcc ataggaatat tactggcttc agaatttgtc gtcaattgat aaggataatc
     6481 agaaatatct acaggagtgg agagggtaat acttccgttt tccaaagctt catagaccag
     6541 ataaacagta atcaattttg ttatggaagc aatttcgaca ggttgcgttg catccttctc
     6601 atagagaatt ttaccagtat ttgcctcaac agcaatcgca tgtttagcgg caatggtaaa
     6661 atcttgagca acagcagtag aagcaccccc taaaagagag acagttaaca aagttaaaaa
     6721 tatttttttc atagtagttt tattctatca taaagaaaaa aaatattctt gctttaataa
     6781 ttcatctgtt aagctttttg aaaatatggt aaaataaagt gagggaggta actcatgttt
     6841 cgtagaaata aattattttt ttggaccaca gaaattttac tcttaaccat catcttttac
     6901 ctatggagac agatggggtc tttgattaac ccttttgtta gcgtgcttaa tacaattatg
     6961 atcccatttt tattaggggg ctttctttat tatttgacaa accctattgt tactttctta
     7021 aataaagtct gtaaactcaa tcgtttgctt ggtattttaa ttaccttgtg tactttggtc
     7081 tggggaatgg tcataggtgt tgtctatctc ttacctattt tgattaatca gttatctagt
     7141 ttgattatat ctagtcaaac tatttatagt cgagtacaag acttaatcat agacttatct
     7201 aattatcctg cgctccagaa tttggatgta gaagctacaa ttcagcagtt aaacttatcc
     7261 tatgttgata ttcttcaaaa tatcctaaat agcgtatcaa atagtgtggg gagcgtcttg
     7321 tcagctctta tcagtactgt tttgattttg attatgactc cagttttttt ggtttatttc
     7381 ttattagatg gacataaatt cttgcccatg cttgaaagaa cgattctaaa gagggatcgc
     7441 ttgcatattg caggcttatt aaagaattta aatgcgacga ttgctcgcta tattagtgga
     7501 gtttcgattg acgcaatcat tataggttgt ttagcttata ttggctatag tattattggt
     7561 ttaaaatatg ctttagtttt tgccattttt tctggtgtag ccaatttaat tccttatgtg
     7621 gggccaagta ttggtttgat tcctatgatc atcgcaaata tattcactga tccccataga
     7681 ctgctgattg cagtgattta tatgcttgtt gttcagcagg tagatggcaa tatcttatat
     7741 cctcgaatcg taggaagtgt tatgaaggtt catccaatca cgattttagt tttacttttg
     7801 ttgtcaagca atatctatgg tgtagttgga atgattgtcg cagtgccaac ctattctatc
     7861 ttgaaagaaa tttctaagtt cttatcccgt ttgtatgaaa atcataaaat aatgaaagaa
     7921 cgagaaagag aattagctaa gtaaaagtca ggagaaccct gatttttctt tactggaagt
     7981 ggcctttaga ttagaagact gaaaataagt taaagtctta aactaatttt cacagctaag
     8041 aatagtagaa gttaatctga taaaaatcga aaaaaccagt ggaattccgt gtcagggtaa
     8101 gttccactgg ttttcatagt ctattaaagt tcgaatgaaa cctatttata gtagattgaa
     8161 actagaatag tacacctcta attctaaaac attgttagaa atcgatttga ctgtcctgat
     8221 ctattcgtcc tattctta
//
LOCUS       DAVMHG010000095         8104 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMHG010000095 DAVMHG010000000
VERSION     DAVMHG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.095"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..1774
                     /locus_tag="VYR08_001558"
     CDS             104..1774
                     /locus_tag="VYR08_001558"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV3467065.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2102..3277
                     /gene="mutY"
                     /locus_tag="VYR08_001559"
     CDS             2102..3277
                     /gene="mutY"
                     /locus_tag="VYR08_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV3467066.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            3332..4036
                     /locus_tag="VYR08_001560"
     CDS             3332..4036
                     /locus_tag="VYR08_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467067.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            4029..5378
                     /gene="vicK"
                     /locus_tag="VYR08_001561"
     CDS             4029..5378
                     /gene="vicK"
                     /locus_tag="VYR08_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV3467068.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            5380..6189
                     /locus_tag="VYR08_001562"
     CDS             5380..6189
                     /locus_tag="VYR08_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV3467069.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            6280..6522
                     /locus_tag="VYR08_001563"
     CDS             6280..6522
                     /locus_tag="VYR08_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV3467070.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            6524..6778
                     /locus_tag="VYR08_001564"
     CDS             6524..6778
                     /locus_tag="VYR08_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV3467071.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            6853..>8104
                     /locus_tag="VYR08_001565"
     CDS             6853..>8104
                     /locus_tag="VYR08_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV3467072.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDL"
BASE COUNT         2503 a         1401 c         1832 g         2368 t
ORIGIN      
        1 ttaaaaacag agatatttac gaacgtttag agagttttaa gatgttaaaa atcttgtttt
       61 gtgatataat gaattatcat ataagaggtt agaggaattt tgaatgaaaa cagatattga
      121 aatcgcacag agtattgagt tgaagccaat tgttgatgtt gtagagaaac ttggtatttc
      181 ttacgacgat ttggagttgt acggaaagta caaggctaaa ctcagctttg ataaaattcg
      241 ggcagttgag agcaatccag tcggtaaatt gatcttggtt actgccatca acccaacacc
      301 tgcaggtgaa ggaaagtcga cgcttaccat tggtcttgcg gatgccttga acaagattgg
      361 caagaaaacc atgattgcta tccgcgaacc gtctcttggt ccagtcatgg ggatcaaggg
      421 tggtgctgct ggtggtgggt atgcacaagt tctgccaatg gaagacatca acctccactt
      481 tactggagat atgcatgcta ttacaactgc caacaatgcc ctttctgcct tgattgacaa
      541 ccacttgcac caagggaatg agctgggaat tgatcaacgt cgtatcctct ggaaacgcgt
      601 tgtggacttg aacgaccgtg cgctccgcca tgtgactgtt ggtcttggtg gtcctctaaa
      661 cggtattcca cgtgaggatg gttttgatat tacagttgct tcagaaatca tggcaattct
      721 ttgcttggca acggacatcg aggacttgaa acgtcgtttg gcgaatatcg ttattggtta
      781 tcgctatgac cgtacgcctg tttctgtagg tgatttgcag gttgagggtg ccttggcttt
      841 gattttgaag gatgctatta agccaaactt ggttcagaca atttacggta cacctgcctt
      901 tgtacacggt ggtccatttg ccaatatcgc tcatggctgt aactctgttt tggcgacgac
      961 aacagccctt cacttggctg attacactgt tactgaagct ggttttggtg cggaccttgg
     1021 tgctgagaaa ttccttgata tcaagacacc aaacttgcca acatctccag atgcagttgt
     1081 tattgtcgca acccttcgtg cccttaagat gaatggtggt gtggctaaag acgctctgac
     1141 tgaagaaaat gtagaggcag ttcgtgcagg ttttgctaac ttgaaacgcc acgttgaaaa
     1201 tatccgtaag ttcggtattc cagcagttgt agctattaac gaatttgtat ctgatacaga
     1261 agctgaaatt gcagtcttaa aagaactctg tgcctcaatc gatgtaccag ttgaattggc
     1321 tagtgtctgg gctgatggag cagaaggtgg agtagcactt gccgaaacag ttgttaagac
     1381 aattgctgaa aatccagcta actataaacg tttgtatgat aatgaccttt ctgtccaaga
     1441 aaagattgaa aaaattgtca ctgaaatcta tcgtggtagc aaagtgaact ttgagaagaa
     1501 atctcaaaca caaattgctc aaatcgttca aaacggttgg gacaaattgc caatctgtat
     1561 ggctaaaact caatacagtt tctcagacaa tccaaatgcg cttggagcac cagaaaactt
     1621 tgaaattacc attcgtgaat tggtaccaaa attaggtgca ggcttcatcg ttgccttaac
     1681 tggtgatgtt atgaccatgc caggtcttcc aaaacgtcca gcagctctca acatggatgt
     1741 tgaaagcgat ggaactgtac taggcttgtt ctagtggatt gaaattgact ttaagtgagt
     1801 ttggaaataa tatccaagct cattttttct cagactgcaa gcagttcatc aataagattt
     1861 atactcttcg aaaatctctt caaactacgt cagcttcacc ttgccgtagg tatatgatac
     1921 tgactctgtc agttctatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct
     1981 tcctagtttg ctctttgatt ttcattgagt ataagtagat agaaaagtta tatactatct
     2041 attgaaaata ttataaatca gatacaaaga caggtgaaac cttctgtgtt atactagaga
     2101 tatgttagat ttgaaagaat acggtatcgt catgtggccg gaggagaaag tcatttcttt
     2161 ccgtgagaaa cttctcgctt ggtatgatga aaacaaaaga gatttgcctt ggaggagaag
     2221 taaaaatcct tatcacatct gggtatctga aatcatgctt cagcagacca gggtggatac
     2281 agttatccct tactacgaaa gattcctgga ctggtttcca actgtcgaaa gtctggcaac
     2341 tgcgcctgag gagagtttac tgaaagcttg ggagggcttg ggctattatt ctcgagttcg
     2401 caatatgcag gctgcagccc agcagattat gactgacttt ggtggccaat ttccaaatac
     2461 ctatgaagga atttccagct tgaaagggat tggaccttac acagcaggag ccatttccag
     2521 tattgctttt aacttgcctg agccagctgt agatggtaat gtcatgcggg tcttggcgcg
     2581 tctgtttgaa gtcaaccacg atattgggat tccaagtaat cgaaaaattt ttcaggcaat
     2641 gatggagatc ttgattaatc cggatcgtcc gggtgatttt aatcaagcct tgatggactt
     2701 agggtctgat attgagtctc ctgtaaatcc cagacctgaa gaaagcccag tcaaggactt
     2761 tagtgcggca tatcagaatg gaaccatgga ccgttatcca attaaatctc ctaagaaaaa
     2821 gccggttcct atttatctta aagccttagt tgtgaaaaat tctcaaggac aatttttact
     2881 tgaaaaaaat gaaagcgaaa agctcttggc aggtttttgg catttcccct ttatagaagt
     2941 tgataacttt tcgcaagaag agcagtttga cctctttcat caggttgcgg aagaaagtgt
     3001 gaactttggc cccagtccag aagagagttt ccagcaggac tatgacctag atgttgattg
     3061 gcttgatgtt tgttttgata cagtccagca tgtttttagt catcgaaaat ggcatgtaca
     3121 gattgtagca ggtcaggtga gtgacttcca tgatttttca gatagggaag ttcgttggct
     3181 ttcaccagaa gaattcaaga attacccact tgccaaaccc caacaaaaaa tctggcaggc
     3241 ttatgcacaa gccaacttag acagtagcca agactagcta ttgtgtcttc tgactatttt
     3301 ttggtataat agtagagaaa aaggtgaaca tatgaaaaaa atactaattg tagatgatga
     3361 gaaaccaatc tcggatatta tcaagtttaa tatgaccaag gaaggttatg aagttgtaac
     3421 tgcttttaat ggtcgtgaag cgctagagca atttgaagca gagcaaccag atattattat
     3481 tctggatttg atgcttccag aaattgatgg tttagaagtt gctaagacca ttcgtaagac
     3541 aagcagtgtg cccattctta tgctttcagc caaagatagt gaatttgata aggttatcgg
     3601 tttggaactt ggggcagatg actatgtaac gaaacccttc tccaatcgtg agttgcaggc
     3661 gcgtgttaaa gctcttctgc gtcgttctca acctatgcca gtagatggtc aggaagcaga
     3721 tagtaaacct caacctatcc aaattgggga tttagaaatt gttccagacg cctacgtggc
     3781 taaaaaatat ggcgaagaac tagacttaac ccatcgtgaa tttgagcttt tgtatcattt
     3841 agcatcgcat acaggtcaag tcatcacgcg cgaacacttg cttgagactg tctggggtta
     3901 tgactatttt ggtgatgtcc gcacagttga tgtgactgta cgacgtctgc gtgagaagat
     3961 tgaagatacg cccagccgac cagagtatat cttgacgcgc cgtggtgtag ggtattacat
     4021 gagaaataat gcttgattta ctgaaacaaa ccatttttac cagagatttt atctttatcc
     4081 tgattttgtt aggtttcatc cttgttgtga ccctcttatt actggaaaat agacgtgata
     4141 atattcagtt gaagcaaatc aatcaaaagg ttaaagattt gattgcagga gattattcca
     4201 aggttcttga tatgcaaggt gggtctgaaa tcaccaatat taccaataat ttgaatgact
     4261 tgtcggaggt tattcgtctc actcaggaaa atctagaaca agagagtaag aggctaaata
     4321 gtattctgtt ttatatgaca gatggggttc ttgcgactaa ccgtcggggt cagattatca
     4381 tgattaacga tacagccaag aagcaactgg ggttggttaa ggaagatgtt ctgaatagaa
     4441 gcattttgga attgctcaag atagaagaaa actatgaatt gcgtgatttg attacccaaa
     4501 gtccagaatt gttgctagat tcccaagata tcaatggcga atatttgaac cttcgagttc
     4561 gctttgcctt gatacgtcga gagtctggct ttatttcagg tttggtggct gttttgcatg
     4621 atacgacgga gcaggagaag gaagaacgcg aacgaagact ctttgtttcc aatgttagcc
     4681 atgagttacg gactcctctg actagcgtaa aatcctatct tgaagccttg gatgaggggg
     4741 ctttgtgtga aactgtagca ccagacttta tcaaggtttc tcttgatgaa accaaccgta
     4801 tgatgcgcat ggtgacggat ctcctccatc tttcacgtat tgataatgct accagtcatc
     4861 tagatgtgga actgattaac ttcactgctt ttattacctt tatcctcaat cgttttgaca
     4921 agatgaaagg acaggaaaag gaaaaaaaat atgagttggt gagagattat cccatcaatt
     4981 ctatctggat ggaaattgat acagataaga tgacgcaggt tgtcgacaat attttaaata
     5041 atgctattaa gtattcgcca gatgggggta aaatcactgt cagaatgaag acaactgaag
     5101 accagatgat tttatccatt tctgaccacg gtttggggat tcctaagcag gatttaccac
     5161 gtatctttga ccgtttctat cgtgtggatc gtgctagaag tcgtgcacaa ggtggtacag
     5221 gtctaggact gtctatcgct aaagaaatta tcaaacaaca taagggcttt atttgggcca
     5281 agagtgaata cggcaagggt tcaaccttta ccattgtgct cccttatgat aaggatgcag
     5341 tgaaagaaga agtatgggag gatgaagtag aagactagaa tgagtgaaat aggctttaaa
     5401 tacagtattt tagcgtcggg ttccagtgga aattcttttt atctggaaac ctcaaaaaag
     5461 aagcttttag tagatgcagg cttgtctggc aagaaaatta ccagtctgct agctgaaatt
     5521 aaccgtaagc cagaagacct ggatgccatc ttgattaccc atgagcattc agatcatatc
     5581 catggagtag gcgttttggc tcgcaagtat ggtatggatc tttatgccaa tgaaaagacc
     5641 tggcaagcta tggaaaatag taaatatctt ggcaaggtgg attcttcgca aaagcatatt
     5701 tttgaaatgg gtaaaacaaa aacttttgga gatatcgaca tcgagagttt tggtgtaagc
     5761 catgatgcag tcgcaccgca gttttatcgc tttatgaagg atgacaagag ttttgtcctt
     5821 ttgacagata caggttatgt cagtgaccgt atggcgggca ttgtcgaaaa tgcggatggc
     5881 tatcttatcg aggccaacca tgatgtagag attttgcgat caggttctta cgcttggcga
     5941 ctcaaacaac gaatcctatc tgaccttggt cacctttcta acgaggacgg tgctgaagct
     6001 atgattcgga cgctaggaaa tcgtactaag aagatttacc ttgggcattt atctaaggaa
     6061 aacaatatca aggaactggc tcatatgacc atggtcaatc agctggctca agctgatctg
     6121 ggagtcggag tagactttaa ggtttatgat acctcaccag ataccgcaac accattgaca
     6181 gagatataga aagaacgctg agaaggtgtt ctttttatat tgactgaaca cctaaaaagt
     6241 aatacaatgg tgttaccatt aaaaaaggga gcacaaaaga tgactactat tacattaaaa
     6301 gtttctgaag ctgataaaac atttatgaaa gcaatggcta agtttgaagg agtttccctt
     6361 tcggaactta ttcgcaccaa aactcttgaa gccctagaag atgaatacga tgctcgtgtg
     6421 gcagatttag cctatcaaga gtatttagaa gacttggaaa aaggagttga acccattact
     6481 tgggaagaaa tgatgcatga tttaggcttg aaggatgaat aatttgtata aattagttcc
     6541 aacaagacgt tttatcaagc aattgaaaaa attggaccgt tatacgcaga agctaattac
     6601 aaactattta caaaccaatg ttttggaaga tccaagacga cacggaaagg ctttggttgg
     6661 taatcgcgtt ggtcaatggc gctatagaat tggtaattat cgagttatcg tacaaattgt
     6721 agatgatgaa ttagttgttg ctactctaga agttggtcat cggagagata tttattgaat
     6781 tacttttttc aagtaaattt tcattcttta aaatatgata taatagggct tacaatataa
     6841 gagaggttgc tgatgacaat agatattagt gaagaaagtt tggctaaaga atcggctgac
     6901 ttattaaaaa ttcttttaaa agatcgaacg acaaagaaat caattgtttg ggctacgcat
     6961 tcttatgaat tgttaggaaa gggatttgct ccaagtgatc gcatcaatcc aagtaaggtt
     7021 actggaaact ttgccaactt gattcagccc agatctgaaa aatccaaata tgaacaaaaa
     7081 gatcggacaa aaataagggc agaagtgttt acaccaacgt ggttggtcgc aaagcaaaat
     7141 ggatatgtag aatcgaaatt aggctcgtta agtcttgagg agtatgtgga cttacgatgg
     7201 ttagaggtaa cttgtggtga agcaccttat atggtaacga gatatgatac cgtaactggt
     7261 gaggaaattc cattatctga gcgagttggt tttgttgata gaaaactgca acggattagt
     7321 cgtgaggttt cagatgaagt cacattttat aaacttgtaa aagatgctta tcatgcttcg
     7381 tatggttatg agtatcaagg agattctctc cttttggcgc gtgagaatct tttggcaact
     7441 tttgaggact attatctagc taagactgga aatcaaccaa cattagagca aaagaaagaa
     7501 attgcgacta ttatctctta caatgtcttt cagatggatg gattgaaaaa aagtagtcct
     7561 tattcagcta aacaaaagca atctcaacaa ttgagtttgt ttgcggatga gctagaagtc
     7621 caagaagtag aagaatctaa aacccatatc aaagactgga aaaagaatag aatgataggt
     7681 tttgagcgcc tatctagcga ggaaagtgaa atgaaatttg atgtcgtgat aggaaatcct
     7741 ccgtatcagg aaacaggaga agccagagat gagccgatat atcactattt tattgatgaa
     7801 gcttataaaa tcgcagataa atctatacta atcaccccag caaggttttt atttaaagct
     7861 ggtcaaactc ctaaaaattg gatggaaaag atgcttgaag acaagcattt aaaagttcaa
     7921 ttctatgaac ttaaaagcgg taaagtattt acaggaacgg atattaaggg tggagtagca
     7981 ataacttatc gtgatgttga taaagatttg ggaccaatcg gagtatttac tatttttgaa
     8041 actttgaatg gtattgtaaa aaaaatcgaa aaacatccag aatttgaaac tttttctgat
     8101 ttag
//
LOCUS       DAVMHG010000096         8061 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMHG010000096 DAVMHG010000000
VERSION     DAVMHG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.096"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..349
                     /locus_tag="VYR08_001566"
     CDS             <1..349
                     /locus_tag="VYR08_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467073.1"
                     /translation="PEENVNIIWKEISNLSLKNDRVIEGYSKIDAYVVNNHEVKAYVE
                     TRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVWHFLCHLDPMFVEIED
                     VEGYVKEEVGEIQ"
     gene            671..859
                     /locus_tag="VYR08_001567"
     CDS             671..859
                     /locus_tag="VYR08_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV3467074.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            921..1679
                     /locus_tag="VYR08_001568"
     CDS             921..1679
                     /locus_tag="VYR08_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV3467075.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1737..2591
                     /locus_tag="VYR08_001569"
     CDS             1737..2591
                     /locus_tag="VYR08_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV3467076.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2703..3587
                     /locus_tag="VYR08_001570"
     CDS             2703..3587
                     /locus_tag="VYR08_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV3467077.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDDYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3750..4523
                     /locus_tag="VYR08_001571"
     CDS             3750..4523
                     /locus_tag="VYR08_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV3467078.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESASNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNYIGSQSLQ"
     gene            4590..5411
                     /locus_tag="VYR08_001572"
     CDS             4590..5411
                     /locus_tag="VYR08_001572"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467079.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAV
                     MNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTA
                     NEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFM
                     KRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEK"
     gene            5764..6219
                     /locus_tag="VYR08_001573"
     CDS             5764..6219
                     /locus_tag="VYR08_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467080.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            6664..7140
                     /locus_tag="VYR08_001574"
     CDS             6664..7140
                     /locus_tag="VYR08_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467081.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            7140..7901
                     /locus_tag="VYR08_001575"
     CDS             7140..7901
                     /locus_tag="VYR08_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEV3467082.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         2785 a         1153 c         1668 g         2455 t
ORIGIN      
        1 tcctgaggaa aatgtaaaca ttatttggaa agagatttca aatttatccc tcaaaaatga
       61 tagagtgatc gaaggatatt caaaaattga tgcatatgtc gttaacaacc atgaagttaa
      121 agcctatgtc gaaacaagag aaacgaacta tcgtaaagca aaagacttct tagaaagccg
      181 cggatatgaa atcgacagaa gctttttcgg aagtgaagac ggtgaagcga ttctttacag
      241 aaaaaaaggg atcgaagttt ggcatttctt gtgtcactta gacccaatgt ttgtagagat
      301 tgaagatgta gaaggatatg ttaaagaaga agttggagag attcaatagt caaccaaagt
      361 tccttgtgag tatatacctt tgctagtaaa agagtcaaaa cgttataacg aaggggggga
      421 agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct
      481 cataatttat actagatttg ataactgatt tgagacagat tttatttcta taaatattgt
      541 gaaaattttt taataccaaa gatttaaagt tactgtgtta aaaatcctct ccacttttaa
      601 actaatgtat tataaaagcc agttgacaca agtgttacac ctgtgataga attaacttga
      661 ggtgatgaaa atggcaaccg ataaaaatag aataatgatt agcttggatg ataaaaatct
      721 agagaaactt gaaaatttag tagaagatgc tagagataga aggggaatgc gactaacaaa
      781 gtcccaggtt attgaattac tcctaaatac cgttgattat ttcgatgata ttatgggggc
      841 tatttactca aaaaaataag agcaaaattg ctattatttt ttacaaattg gtgtaacact
      901 agtgatatag gagtgatgaa atggttgttt atattagaca aagtaaatta ccaagtgagg
      961 tttcaattaa taaatacaat gctcaagtag gtgcctactt acaaggagaa gaagctgttt
     1021 tatatcaatc tttttctgaa ataaaagagt taacaagtga agatatagtt gtagattata
     1081 taatggagac tagagcatta ctaaaaatga tgggcttaaa cgttccggtt catgactatc
     1141 ctattgagct taaagagttt tatggtcgaa agatttatgc tggtatttta ggagagattg
     1201 tgaatatacc tgataattgg ggaaaattta ttaagcctaa agctggttca aaagtcttta
     1261 ctggaagagt tgttaatgga acccatgatt taataggtat tggtctacct ttcgactatc
     1321 ctatatggat tagtgaggtt gtagaattca tagctgaatg gcgttgtttt gtgttagatg
     1381 gtcgcgtatt agatgttcga ccctatacag gtgattatca tgcacaattt gatgcaagtg
     1441 taattgatga agcgatatca tgttggaaag atgcgccaat agcttatgga ctagatatcg
     1501 gtgttactcg cgatggcaga acacttgttg ttgaagtaaa tgatggttat gcattgggaa
     1561 attatggcct atctccttta aaatcaatca attttcatag agctaggtgg aaagaaatgg
     1621 taaaacccta ttttgaaaaa aatgaaattt ttaaaatcca acaggatgtt attttctaaa
     1681 ataaatatgg tagataacca atataaaaag catcaaaatg aacaaggagg aatctgatgc
     1741 ttggagcaat tattggagat attgtcggtt ctgtttacga atggaacaat atcaaaacaa
     1801 aggactttcc tttatttcgg aaggactgct tttttacaga tgacacggtt atgacctgtg
     1861 ctgttgcaga agcaattatg aatggtggac aaaaagacga tttcattgac gcgatgaaga
     1921 aatatggcag aatgtatccg aatgctgatt acggtgctcg gtttaatgca tggctaaaca
     1981 gcgataaccg tgagccttat aatagctttg ggaatggatc agctatgcgt atttctccat
     2041 gtgcttggat catggactgt ggtttttatg cgaaaactgg tatgtggcca tcatctagag
     2101 gacttacgag tctttctgca gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta
     2161 tggctacagc tgatgctatc tttctatgtc gtttttactt tggtggttat tgtagagagt
     2221 acgagcaatc aattaacgac aatcctacag agtgtaaaag acgaattaag gattatatag
     2281 aaaaagaata cgattacaat ctatctcaaa ctttagatga aatccgtcct aactatcgtt
     2341 ttaacgaaac atgtcaggaa actgtccctc aagccattat cgcctttctt gaaagtagag
     2401 acttcgaaga tgcgataaga aatgccatct cacttggtgg cgacagtgat actctcgcag
     2461 caatcacttg tagcatagca gaggcagctt acggtattcc tgattggatc aaggataagg
     2521 cctattctta cttggatgaa cccttaaagg atgtagttag gcgatgggaa aatagaataa
     2581 aagcgtatta atctccagta cgtcagattt ctcttttctg tattttcatt gtataatagg
     2641 attacattta cttgcatacg acgaaatgta aacatttctg tttaaaatta aggataaaat
     2701 caatgaaaaa agcaatggta attatcaacc ctacttctgg tggcgagaag gctttggatt
     2761 acaaagaaaa gctggagaat aaagcaaaag aatactttga atatgttgaa actaaaatta
     2821 ccgaaaaagc gctggatgca acacattttg ctgaagaagc ttctcgtgag cagtatgatg
     2881 cagtggttgt gtttggtgga gatggaactg tcaatgaagt catttcaggt attgatgaga
     2941 gagactacat tcctaagtta gggattatcc caggcggtac gggtaacctc attacaaaac
     3001 ttttggaaat caatcaagac atcgatggcg caattgacga actggatttt gatttaacca
     3061 ataagattga tatcggtaaa gcaaatgaca actattttgg ttatatcttt agtatcggtt
     3121 ctctgcctga ggcgattcac aatgttgaaa tagaggacaa aacaaaattc ggtattttaa
     3181 cctatgctgt aaataccatg aagtctgtca tgacagatca ggtctttaac attaaggttg
     3241 agacagaaaa tggaaattat gttggtgaag ctagccatgt tttggtcctt ttgacaaatt
     3301 acttcgctga taagaaaatc tttgaagaaa acaaggacga ctatgccaac attttgattc
     3361 tgaaagatgc ctctatattc tccaaattat ccgtcattcc tgatttatta aaaggggatg
     3421 ttgtcgcaaa tgataatatc gagtatatca aagcgcgtaa tattaaaatc tcttcagata
     3481 gtgaattgga gtcagatgtt gacggagata aatcagataa cctacctgta gaaatcaaag
     3541 tcctagctca gcgagtagaa gtattttcaa aaccgaaaga ggattagtat atagagaaag
     3601 ccttttttaa ggctttttgt atactttaaa agatagttcc tttaacaacg gacattcctt
     3661 gcaaatagtt tacaaaaata gtatactgga ttcattgagt ttgaaaacgt ttgcgtaaaa
     3721 tttgaatgaa tactttagga gacaaattga tggaattgag tgctatttac cataggcctg
     3781 agtcggagta tgcctatctt tataaggata agaaactcca tattcgaatt cgaactaaga
     3841 aaggggacat tgaaagcata aacttgcact atggggaccc ttttatcttt atggaggagt
     3901 tttatcagga tacaaaagaa atggtcaaga taacttctgg taccttattt gaccattggc
     3961 aggttgaagt gtcagttgac tttgcacgta tccagtatct ctttgagctc agagatacag
     4021 aaggtcaaaa tattttgtat ggcgataaag ggtgtgtgga aaattctcta gaaaatcttc
     4081 atgcaattgg gaatggattt aagttgcctt atcttcatga gattgatgcc tgcaaggttc
     4141 ctgactgggt ttcaaatacg gtatggtatc agatatttcc tgaaagattt gccaatggca
     4201 atgctctatt aaacccagaa gggactttag actgggattc atctgtcaca cctaagagcg
     4261 atgatttctt tggtggtgat ttacagggga ttattgatca tatggattac ttgcaagact
     4321 tgggtattac tggactatat ctttgtccca tctttgaatc tgcaagcaat cacaagtaca
     4381 atacgacaga ttactttgaa attgaccgtc attttggaga caaggagacc tttcgggaac
     4441 tggtggatca agcgcatcat cgtggcatga aagtcatgct ggatgcggta tttaattata
     4501 ttggttcgca atctcttcaa tgaaaaaatg tcgtcaaaaa tggtgaacag tctgcttata
     4561 aggattggtt ccatattcaa caattcccag tgacaactga aaagctagtt aataagagag
     4621 acttacccta tcatgttttt ggtttcgagg attatatgcc taagctaaat acagccaatc
     4681 cagaggtcaa gaattatctt ttaaaggttg cgacttattg gattgaagag tttaatatcg
     4741 atgcttggcg tttggatgtg gctaatgaga ttgaccatca gttctggaag gattttcgta
     4801 aggcagtttt agctaaaaat cctgatcttt atatcctagg agaagtctgg catacatctc
     4861 agccttggct aaatggagat gagttccatg ccgtcatgaa ttatccttta tctgatagta
     4921 tcaaggacta tttcttacga ggaattaaga agacagacca gttcatcgat gaaatcaatg
     4981 gagagtctat gtattacaag cagcagattt cagaggtcat gtttaatctc ttggattcac
     5041 atgatacaga gcgaatcctg tggacggcca atgaagatgt tcaactggtt aaatcagcct
     5101 tagcctttct ctttttacaa aaaggaacac cgtgcattta ttacggaacc gagctagcct
     5161 tgactggagg accagatcca gattgtcgtc gttgtatgcc ttgggaacgt gtatcaagtg
     5221 acaatgatat gctgaacttt atgaagaggc tgattaagat tcggaaatac gcgtcagtaa
     5281 tcatttcgca tggcaagtat agccttcaag aaatcaaatc tgatctagta gctctggaat
     5341 ggaaatacga aggacggatc ctcaaagcaa tattcaacca atcaacagaa gattatcttt
     5401 tagagaaata agcagtagca ctagcaagca attgccaaga attggagaat cagcttgtca
     5461 tctctccaga tggatttgtg attttctaaa aactagttga tgaagattat ggtacatttc
     5521 atatcttata tagtataata aggctagtta ctaaacttgt aaaggagaac ttaaatgaat
     5581 tgtagaggac atgaaacaag acaaagaatt gttagagatt ttgaagttta gcctaaagca
     5641 catattaagc tgttagcaaa tcaacaaaaa catagtgatg caggagcaac tattgaagat
     5701 gaatattatg tatttatcgc tgagagtaaa attgatggca agaaggaagt tattcagtgt
     5761 tgcatgggtg cggcaaggga ttttttagaa ctaattaatc acaaagggct acctcttttt
     5821 aatccgcttg taggtgattc tcatgtaaat aatagacaag aatatgacaa tacagggagt
     5881 ggaaatttac aacctgaaaa gtggaatgaa actgcaaagc agctttataa tgttataatg
     5941 tggttgatta ttttatggaa tgctaagccg gatacacctt tatttaattt taaagacgaa
     6001 gtaattaagt ataaaacata tgagcctttt gaaagcagta taaaaagagt aaatactact
     6061 ataaagaatg gtagtaaagg gaaaactctg actgagatga ttaatggcta cagagcggat
     6121 aacgatatta gagatgaaat ttgtaacttt aatattctga aaaataaaat tcgtgatatg
     6181 aaagaccaac aaggaaatac aatggaatct tacttttagt tattgttgaa ttttgggtat
     6241 tctataaaat atcctaattg agatttaaat agtagactat acaatatagt taaaatatca
     6301 gtaaaaacaa cactttattg aggtattgga tacgctttgc taatagccta ataatcacat
     6361 gtggagtgtt gctacaacga aaaaggtgat aatccttgat ttcaagctat tttataagca
     6421 ttttgtcttt gtagataaag gcaattttga caataaaaat cctaaaaggt gaatcgttat
     6481 agatgtattt gtagatatcg tttgcgcatc gaaaaaatta atacaagaat aaatatttat
     6541 agctctttag gtgactttta tagaagtaaa gtttaggata gaaaaacaag aaataacgca
     6601 ccatttttgg tgcgttatgc ttttttatgc tataatggat ttataaaaat aaaggagttt
     6661 gctatgattg gaaagaacat aaaatcctta cgtaaaacac atgacttaac acaacccgaa
     6721 tttgcacgaa ttataggaat ttctcgaaat agcttgagtc gttatgaaaa tggaactagt
     6781 tcagtctcta cggaattaat tgacatcatt tgtcagaagt ttaatgtatc ttatgtcgat
     6841 attgtaggag aagataaaat gctcaatcct gttgaagatt atgaattgac tttgaaaatt
     6901 gaaattgtga aagaaagagg tgctaatcta ttatctcgac tctatcgtta tcaagatagt
     6961 cagggaatta gcattgatga tgaatctaat ccttggattt taatgagtga tgatctatct
     7021 gacttgattc atacgaatat ctatttagta gaaacttttg atgaaataga gagatatagt
     7081 ggctatttgg atggaattga acgtatgtta gagatatctg aaaagcggat ggtagcttaa
     7141 tggaaatcca agattatact gatagtgaat tcaaacatgc tttagcaagg aatcttcgtt
     7201 cactgacaag aggaaaaaag tccagtaagc aacctatagc gattttgctt ggagggcaaa
     7261 gtggtgccgg taagactaca attcatcgta ttaaacagaa agaatttcaa ggaaatattg
     7321 ttatcataga tggtgatagt tttcgttctc agcatccaca ctatttagaa ctgcagcaag
     7381 aatatggcaa agacagtgta gaatatacca aagattttgc aggaaaaatg gtagagtctt
     7441 tagtaacaaa attgagtagt ttgagataca atcttttgat agagggaact ttacgaacag
     7501 ttgatgttcc aaagaaaaca gcacaactct tgaaaaataa gggatatgaa gtacaattgg
     7561 ccttaattgc gacaaagcct gaattgtcgt atctaagtac tcttatccgt tatgaagaac
     7621 tgtacattat caatccaaat caagcacgcg caactccaaa agaacatcat gatttcattg
     7681 taaatcatct agttgataac acacgaaaat tggaagaact agctatcttt gaaagaattc
     7741 aaatttacca acgagataga agttgtgtat atgattcaaa agaaaataca acttcagcag
     7801 cagatgttct tcaagagtta ctctttgggg agtggagtca ggtagagaag gagatgttgc
     7861 aggtggggga aaagagactt aatgaattac ttgaaaaata aacaattgat atttttagga
     7921 gaatagaaat gagagggttt aataacaaga taaagtctgt ttatcaagaa ctaacaaatt
     7981 ccaaagagaa attcggtagc tttcacaaga ctttaattca tttgcataca cctgtttctt
     8041 atgattacaa gctattttct a
//
LOCUS       DAVMHG010000097         7898 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMHG010000097 DAVMHG010000000
VERSION     DAVMHG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.097"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(49..272)
                     /locus_tag="VYR08_001576"
                     /pseudo
     CDS             complement(49..272)
                     /locus_tag="VYR08_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(275..775)
                     /locus_tag="VYR08_001577"
     CDS             complement(275..775)
                     /locus_tag="VYR08_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467083.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            811..990
                     /locus_tag="VYR08_001578"
     CDS             811..990
                     /locus_tag="VYR08_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467084.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(968..1936)
                     /locus_tag="VYR08_001579"
     CDS             complement(968..1936)
                     /locus_tag="VYR08_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV3467085.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(2022..2237)
                     /locus_tag="VYR08_001580"
     CDS             complement(2022..2237)
                     /locus_tag="VYR08_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV3467086.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            complement(2246..3100)
                     /gene="cvfB"
                     /locus_tag="VYR08_001581"
     CDS             complement(2246..3100)
                     /gene="cvfB"
                     /locus_tag="VYR08_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV3467087.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(3160..3717)
                     /gene="frr"
                     /locus_tag="VYR08_001582"
     CDS             complement(3160..3717)
                     /gene="frr"
                     /locus_tag="VYR08_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV3467088.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(3726..4463)
                     /locus_tag="VYR08_001583"
     CDS             complement(3726..4463)
                     /locus_tag="VYR08_001583"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV3467089.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(4547..5881)
                     /gene="trmFO"
                     /locus_tag="VYR08_001584"
     CDS             complement(4547..5881)
                     /gene="trmFO"
                     /locus_tag="VYR08_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV3467090.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(6126..6380)
                     /locus_tag="VYR08_001585"
     CDS             complement(6126..6380)
                     /locus_tag="VYR08_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV3467091.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            6375..7178
                     /locus_tag="VYR08_001586"
     CDS             6375..7178
                     /locus_tag="VYR08_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV3467092.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDVTEVKINRPKKQLANYYGKKKCYAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILTDSDYQGPMKIYPQAQTPRKASKLKPLTAEDKACNHA
                     LSKDRSKVENILAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(7167..7811)
                     /locus_tag="VYR08_001587"
     CDS             complement(7167..7811)
                     /locus_tag="VYR08_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV3467093.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERARL
                     GLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYIE
                     NNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
BASE COUNT         2290 a         1771 c         1460 g         2377 t
ORIGIN      
        1 taaaatcagg tcttatagaa acatcttatt atcaagttct tccactcaaa tcatttcttt
       61 ggcacctttg tatggaaact caaaagaaga ttggtcaatc ttatctaaga ctgcttgcac
      121 gggtttaact aaaagcgatc gtcataaata ccgccaataa tcttgccgcg gaagtaaaga
      181 atatactccc ccatcatgga acggtaagtc acatcatcta atcctgataa ttgttccaaa
      241 acaaattcca aatagttctt acttgatgcc atttctaatc ttctaggctc tgttcaacga
      301 taacaaccgt atagagttct tgcttaacct cgcatccaat tgatttaaag ccctgctttt
      361 cccaaaaatg ctgagattgc ggatttccct taacataagc caaacgtgcc tttcgaaagt
      421 tcttagcaaa ataagctagt gcttctgtca caatatgact accaatccct ttcctctgat
      481 aggcttgatc aaccataaac aaaccaataa aaacagtctc ctcatcagga tatgcataga
      541 caaaatccat aacagccaca aggtcagatc cattccaaaa tccaacaaaa aacttatcag
      601 ccttagcttt accttcaggt agacaaagca tgtcctcttt tacagttgca aaatttggtt
      661 ctggtggaca atgctgaaaa tacagaggat tactttcata taaagataaa atacttggaa
      721 tatccttttc agttagtatc ctacaactgt aatacttaga tagttggtca atcatctttt
      781 caaattccta ctttcttgtg ccctgtgatt atgacacagg aagatgcact gatcgtcatc
      841 agccacataa aagttctttc catcgtgcct aatcgttgtc tcagaccttt ggataaaacc
      901 tttagcctat acaactggat tttcctctct caaaagtata ttcttttgca ggcgaacttc
      961 ctcaaaatca gtcgtgtgca acttcagtag aatattcata ggctcggata atctgagcga
     1021 caactggatg gcgaaccaca tccttggctg aaaaatgaac aaagtcaatc tgatggatgt
     1081 tcttgagttt ctcttgagca tcaatcaaac cggacttgac attacgtggc aggtcaatct
     1141 gactgatatc tccattgaca atcatcttag aatgaaagcc taaacgcgtc aagaacatct
     1201 tcatctgcat gatggtcgtg ttttgcgcct catcgagaat gacaaaggca tcatccaagg
     1261 tccggccacg catataggca aggggcgcaa tttcgataat ttcacgctcc atgagacgag
     1321 tcgtttggtc tttcccaaga atttgataca aggcatcgta aacaggacga aggtaaggat
     1381 ccaccttctc cttaagatca cccggaagaa atccaagact ctctcccgct tccaccgctg
     1441 gacgagttag gataattcgc ttgacttgcc cacgtttaag ggcagtcact gccaaggtca
     1501 ctgcaaggaa ggtcttccct gtacctgctg gcccaattcc aaaggtcaca tcatgctgtt
     1561 tgacactgtc cacataaagc ttttgcccta gggttttgac acggataggt ttcccagtat
     1621 tatctttgat aatttcttct tcgtaaaggg cgacaaactt gtcaatttca tcatttttga
     1681 ccatgctaat cgcagtgact acatctggcg taccaacggt catcccacga tttaccaaga
     1741 ccatcaaagc ctgaataact tgacgggctt cctcacaggc agactcttct cccaaaacct
     1801 ggacaatctc cgtacgagca tgaatcacaa catcaagctc ttcttccatc aaacgaagat
     1861 ggcgttcatt ggaaccaaaa agatgaaaca ggtcatctgg atgactcagt tgaatgtcta
     1921 ttgaatgttc cttcaaataa agaacctctc taatcctttt atttcttttt attatagcag
     1981 agggatggag aaattactag cccaaaccta gtgaataaat gctagtgttc taagtattct
     2041 tgccagatag cgtcaaagtc tcctaggtta aaagagaaac tggcatgctc ctccaaaaag
     2101 cgactgacct catcaaaatc atctgtgtgt tttggaaaag ctgcctcttc aaaagcgagg
     2161 tctgccaaaa ttgctttggg actgttactt ttaggattgc gctcggtcat gagccaagtg
     2221 taaaatgatt ttctcataag cctccctaaa tcaactctgt cccaaactgg tcctgcttga
     2281 ttttaccagc cttcataaga ccacctaaag ctttcttgaa ctgaccttta gaaatgccaa
     2341 aggttgcctt gatgtcgtct ggagatgact tatcatttaa ggtcatgaaa ccgccattac
     2401 tttccaaata agtcaaaatc atctgagcat cgttttccaa catttcaaag gagcgtggtt
     2461 tgagggagag gttcagagtg cggtccactt cacggaaacc aataacgcgc gcatctaata
     2521 cttgccccaa acgtggctct gcgtaacgct cgctaggatg aataaaacca agcatattat
     2581 tttctggtag gtaaacaaaa gttcctgaca gcttgagacg gtaaacaatg gctggccagt
     2641 tttggttctg catgttgttg taggcaggac gagcaagacg ttggaagtct tcttgataag
     2701 ccaagaggcc ccagatacgg tctttcttat ccacttcaag acggatgtag agttggtcgc
     2761 ccttcttagg ccagagttcc ttgagctcag ggagaatatc gagtgacaca acgatttcct
     2821 tgtcaggaag gcctgtatcc acaaagacac ccaagtcctt acgaacctct gtgacacgtc
     2881 cccaaccaaa ttggtcctga gtggcagtca cttctaaggt tgtcaggcgg agtttttgct
     2941 tcatatccgt gtatgcaaaa cctttgaccg tatcccctac tgtatgttgg ccttcttcct
     3001 tagcaagagc ataggtttga ccatcctttt gcacaaagta aaaacggtcg ttttcatcga
     3061 tgatcagtcc aacgataaaa cttgcaagat ttgtattcat atttccttct ttcgaataaa
     3121 actcagccag caatgccaac tgagtttttc tgtttatttt tagacttcca aaagttcttt
     3181 ctctttgtta gcagtcatgt cgtcgatgtg tttaacagca tcgtctgtta ctttttgaat
     3241 gtctttttca agagtcttca attcgtcttc agtgatttct tttgcttttt cttgtttctt
     3301 agcttcgtcc atagcatcgc gacggatatt gcggacagcc actttagcat tttcgccgac
     3361 cttcttcact tctttagcaa ggtcacgacg agtttcttct gtaagagctg ggataaccaa
     3421 gcgaatcaca gaaccgtcat tagccggtgt gataccaaga tcagaagcgt tcaaggcacg
     3481 ttcgatgtct ttcaatgaag acttgtcaaa tggtgttacc aacaaaacac gcgcttctgg
     3541 aatcgtaatt gaagcgattt ggttaagagg agtttcgact ccatagtatt ctacatgtac
     3601 acggtcaagc aagcttgcat tggcacgacc agcacggata ccaccaaatt cacgagcaag
     3661 tgattggtga gactgggtca ttctctcttt agctttttca ataattacgt tagccatatt
     3721 ctttcttatt ccttttcttc gatattattt gaaactgttg ttccgatatt ttcaccaaat
     3781 acgacacgtt tgatgttgcc tgattggttc atgttgaaga caaccaagtc aatgtcgttg
     3841 tccattgaga gggttgaggc tgttgagtcc atgatacgaa gacctttgtt gataacatca
     3901 cggtgggtca attcttcaaa cttaacggct gtcttgtcct tcttaggatc ggcattgtac
     3961 acaccatcga cgccattttt agccatgagg atggcatctg cttcgatttc agctgcacga
     4021 agggccgctg ttgtatctgt cgagaagtat ggtgaaccaa ttccagcacc aaagataacg
     4081 atacggcctt tttcaaggtg acgaagggca cgtccacgga cataaggctc tgccacttgt
     4141 tgcatagcaa tagctgtttg tacacgcgta tcaaccccaa cttgttgcaa tgaatctgcc
     4201 atcacaagag cattcataac agtcccaagc attccagtgt aatctgcctg aacacggtcc
     4261 atacctgctt ctgctgcagg ttctccacgc cagagatttc ctccaccaat aacaagggca
     4321 atttcgatac ctaagctatg aacttcttga atctcttttg cgattgtttg aactgtttgg
     4381 atatcaatcc ctacgccacg ttcaccggca agggcttcac ctgataactt gattaaaata
     4441 cgtttatact tgggattcgc cattttcact ctccttcttt catcctacct attttatcac
     4501 aatttctaag atttttatag tatcatgaac aattctttca aaaaaattag acagtcaaaa
     4561 attcctctaa gtcggcaagg gcacgctctg caattttttc ataacgagcc ttcttatcac
     4621 ggatacgctc gccttccaac tccttgatga tcccaaaatt gacattcatt ggttggaaat
     4681 gtttgctatc ggcatgggta atgtaatgag ctaagcttcc aatcgctgtc gtctcgggga
     4741 aaataacctc gctttcttcc ttgaagagac gagctgcgtt aattcccgca actaagcccg
     4801 aagccgccga ctcaacatag ccttccaccc ccgtcatctg accagcaaag aagagatttg
     4861 gttgtttctt ggaacggtaa gtctgctcaa gaagatttgg tgaatccatg taagaattgc
     4921 gatgcatgac accataacgg acaaactccg cattttcaag acccggaatc atttggaaga
     4981 cacgcttttg ttctccccat ttgaggtggg tctggaaacc aacaatattg tataggctac
     5041 cagctgcatt atcctgacga agttgcacaa ccgcataagg tgttttaaat tctccatcac
     5101 gaggtcctgt gtagtcgtcc ggatactcaa gaccgactgg cttcataggg ccataaagca
     5161 tagttttaat gccacgtttg gccataactt cgattggcat acatccttca aagtactttt
     5221 ctttttcaaa agaattgagc ggtgcttctt ccgcattgac caaggcttca tggaaatcca
     5281 taaactcttg cttggtcatt ggagcattga gataggctgc ttctccctta tcataacgag
     5341 atttgagata gaccttactc atatcgatag tgtttacatc gataataggc gctgctgcat
     5401 cgtagaaata gaagccatcg ccattattaa gggcatgaat cttttcagcc agggcatcgc
     5461 tagtcaaggg accagtcgcc acaactgtaa taacatctgt tggcaattct gtaatttcat
     5521 cacgcaccac ttcaatcaag gggtggttga ccactttttc ggtcaccatt tgagagaaac
     5581 catcacgatc caccgcaagg gctccaccgg caggaacacg tgtagcctca gcagattcca
     5641 agataacaga acccaagcga cgcatttctt ccttgagaag accaactgca tttgtcaaag
     5701 catccccacg caaagaattg gaacaaacca actcagcaaa attgtctgtt ttatgctggg
     5761 gtgtagactt gacaccacgc atttcatata gtttaactgg aataccacgc tctgcgattt
     5821 ggtaagctgc ttcagaacct gccaaaccag caccgataac attgatataa gattgagaca
     5881 cgacactaat acctctttgg gagtgtgaag ttaagattca cattgaaaaa gccaatcaga
     5941 cttacaagct ttcgagtttc ttggctcagg ctgaaaaagt ccacaggact ttttcactcc
     6001 cacaaatctt tctatttttt cttctactag tataacaaaa aaagggaaga aggcaaactt
     6061 ccctgtttag tcattttctt gatgtaaaga gatagtgagt attccagtta agaatcaatt
     6121 atatgctact ctataaaatc ttttccacat aacggatcga tagggactgt tattctatct
     6181 cttgctataa ccgtattatc taaaacagca taacattcaa catagtgatc tcctttaaac
     6241 tgtgaatctt cgtgatattt tattttacct gaaaaaatag caccacgctc acaatttctt
     6301 ctaatagcct cagcttcaat atttcttact ttccaataga cttcctgcga aacaaaatat
     6361 ggtatagtag ttctatgaat gatgaagcaa gtaaacaact aactgatgca cgatttaagc
     6421 gtcttgttgg tgttcagcgc accacttttg aagagatgtt agctgtgtta aaaacagctt
     6481 atcaacttaa acacgcaaaa ggtggacgaa aacctaaatt aagcctagaa gaccttctta
     6541 tggccactct tcaatatgtg cgagaatacc gcacttatga agaaattgcg gctgattttg
     6601 gtattcacga aagcaactta atccgtcgga gccaatgggt tgaagtaact cttgttcaaa
     6661 gtggtgttac gatttcaaga actcctctca gttctgagga cacggtaatg attgatgtga
     6721 cggaagtaaa aatcaatcgc cctaaaaaac aattagcgaa ttattatggt aaaaagaaat
     6781 gctacgctat gaaggctcag gcgattgtca caagtcaagg gagaattgtt tctttggata
     6841 tcgctgtgaa ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac
     6901 aagctggtaa aatcttgact gacagtgatt atcaagggcc catgaagata tatcctcaag
     6961 cacaaactcc acgtaaagcc agcaaactca aaccgctaac agctgaagat aaagcctgta
     7021 accatgcact atctaaggat agaagcaagg ttgagaacat ccttgccaaa gtaaaaacgt
     7081 ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt
     7141 tgagtgctgg tattatcaat catgaactag gattctagtt ttgcaggaag tctaatgtaa
     7201 ttctcaagct gtgagttcat tatgatttgt aaatccgata tatcttcagc agctaaaaac
     7261 acattttgaa atgttccaag tccgttttca ccaaaactat attctctctt actatactta
     7321 ctcttattat tctctatata gttagagtca aggttcttaa cctcttgaag ttcattttct
     7381 ttacttctga ggttaaaatc ttttacttct gaagttaagt tttttgactt ctgtaagtca
     7441 ttttctttta cctgaaatat actcataaag tctttaacat aaatgatgtt cggcttacca
     7501 agtccaagcc ttgctctttc gatcagtcct attccttttt ttacatcaag ctcatctaat
     7561 gtttttatgg cagttggctt tgagatattt cttcttttca taatttcttc gacagtaaag
     7621 ataaatactc tgccttcctt gtctatccag ttattcttaa atgacattcc tgtctgttta
     7681 agaagtatgg aataaaggat aattgcttct gctgataagc cccgaaattc ttctctatca
     7741 actaatatct ccggtacttt taagaagtta aatctttccg cttctctgtt ataaaaatat
     7801 tcaaagtcca tgcaagatca cctcccttct taaaaatttg caaagaaaaa gacgatagtt
     7861 tttcttctat cgcctttggt aaccattttt atgaaatt
//
LOCUS       DAVMHG010000098         7807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMHG010000098 DAVMHG010000000
VERSION     DAVMHG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.098"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1061..2287)
                     /locus_tag="VYR08_001588"
     CDS             complement(1061..2287)
                     /locus_tag="VYR08_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV3467094.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFVGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2330..3022)
                     /locus_tag="VYR08_001589"
     CDS             complement(2330..3022)
                     /locus_tag="VYR08_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV3467095.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(3195..4946)
                     /locus_tag="VYR08_001590"
     CDS             complement(3195..4946)
                     /locus_tag="VYR08_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467096.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEDLLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4936..6681)
                     /locus_tag="VYR08_001591"
     CDS             complement(4936..6681)
                     /locus_tag="VYR08_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467097.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6887..7183)
                     /locus_tag="VYR08_001592"
     CDS             complement(6887..7183)
                     /locus_tag="VYR08_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV3467098.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(7336..7791)
                     /locus_tag="VYR08_001593"
                     /pseudo
     CDS             complement(7336..7791)
                     /locus_tag="VYR08_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
BASE COUNT         2361 a         1694 c         1613 g         2139 t
ORIGIN      
        1 attgaattgt caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct
       61 tcaaactagg tcagtgccgc cttgccctaa gtctaggtta ctgacttggt cagttctata
      121 gtagactgaa actataatag tacacctcta cttctaaaac attgttagaa atcgatttaa
      181 ctgtcccgat caatttgtcc tattcttgtt tcattttact atatctacaa cctcaaagca
      241 gggttttgag ctatctatgg ccaacttcct agtttgctct ttagccttcc attcaaaagt
      301 gatgctatct ttcttagctt gaatataaaa tatcccattt actatattta aatttgttga
      361 tatgttgtaa attataaatg aaagaacaaa taccttgtca tacaagcatt cgccagggat
      421 atttacctga atatcgctca tatggcggat tgaaactaaa acactacaat atatttctta
      481 aatagacctt ctataaaagc tagatttaac ctcgataaaa atagatgcag tgcttctaaa
      541 atactggaat agggcatata ggtaatcata gacaagtgtt ttatagattg tctcgcagga
      601 gatctaataa tgtatagcaa gcatcactaa acatatagaa tatttgtatt attataatat
      661 caacatgcta taaattctat catgcaaaag aatgactaga acatgaaaga caggcacaaa
      721 attttgaaca atattctgaa aacaaaaaaa gacgcgagtt tccacaatct cactcatgga
      781 aactcacgtc tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct
      841 aaatgatgtc tagtaaggat tctaaatgct acactaaatt gctcacatct tatttcaaac
      901 cacgggactc ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg
      961 cctcaactac aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga
     1021 ctttttaggg acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta
     1081 gcactttttc agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta
     1141 atttagtatg aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt
     1201 tcgtcatatc aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa
     1261 cattacttgc aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc
     1321 ttgctccttc tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt
     1381 gtgccaaagg atggatgcga gaacctacaa aaccactatc atagcggtcc acaatgtcct
     1441 tacggcgttg caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga
     1501 tatcggtcat gttgcactta taggctggtg taacgatatc gtattcccat gaccccaatt
     1561 gcatcttggc aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt
     1621 acatctcttc gtcatcaatc actggattgg ctttccaagt cgcacttcca ccttctgccg
     1681 ttgtaaagtt cttaacggca tggaatgaga aggaagtaaa gtcagcgata gaaccagaag
     1741 gttgtccttt ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt
     1801 taaaggcctt ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt
     1861 ggaacaaacg gtcataatcg caaacaatcc ctgcgagctc tactgggata atcaccttag
     1921 ttttctcagt gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga
     1981 tatccaccat gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg
     2041 tcatggctgg aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact
     2101 ccagagcggc tgtcgcagag ttgagacaaa cagtcttagg tgtctgtgtg taaagagaca
     2161 agcggcgctc cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat
     2221 ccgctacttc agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat
     2281 ttggcattga tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta
     2341 cctcaaacac ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat
     2401 aggcgaggtt atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg
     2461 acagaccttt ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg
     2521 ctggagaggt aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct
     2581 ctgtataacg tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat
     2641 tgaccaactg aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa
     2701 tacggctatc gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg
     2761 tacggaactt ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa
     2821 tcactggccc cttgctatcc aacttgatcc aaatgctcaa gatgagaaag atgggagaag
     2881 ttagaaccag taagaccaag gccaaaaccc agtccaagca acgcttgaaa atcagcgaac
     2941 ccttcctttt agagacaagc tggtagtaag actcaacctc gcttgatttc atttccacag
     3001 gcaagtcttc ccatttcagc atgctgttct cctttgtttt tattatacta tttgtcaaca
     3061 tttttcatta taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt
     3121 ccttctagta ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg
     3181 gtttaaagag attttcaaag agtataggcc atggcccctg cctgcaaact atacatcttg
     3241 tgataggttc ctcccagagt caagaggtcc tcatgggttc cactctcgat aatgcgtccc
     3301 ttatccaaga catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca
     3361 atagttgttc ggccctgtct catcttcgcc agagaagctt gaaccaaact ttctgtttca
     3421 gagtcaatat tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt
     3481 ctagcaaagg caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca
     3541 ggggagtcgt acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc
     3601 gcagcctgaa cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt
     3661 ccatgataga ggaagggttc ctgtaaaacc aaaccgatgt tttttctcag ctcttcttga
     3721 ctgaaatccc tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag
     3781 cgcatgagga cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg
     3841 gtttcaccct tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatac
     3901 gagaaacaca catgttcaaa acggatattg ccttctttga ctttggcttg cccattttct
     3961 tgaaggggct cataggtcct ctcatcgatt aaggcaaaga cacggcctgc agaaaccata
     4021 gacgtttgca gtgttgaaaa gttttgcgtc acctcaatca aagggtcaaa gaggcggttg
     4081 atgtactgga taaaagcata catggtcccg gccgttatcc cgatagaaag accacggtag
     4141 ccaaaatagg tcatcaagac agcataacct agaagtttca acaaactcat ggcaggtctc
     4201 aaaaagaggg catccaaggc tacagaacgg ttggcatagg ccaagtgctc ttggttgatt
     4261 tcatcaaatt ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc
     4321 tcgatattct ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg
     4381 attttcactg acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc
     4441 aagactaatg ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag
     4501 ataaaaactg ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg
     4561 gtatcgttgg tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg
     4621 cccagtttct ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac
     4681 acgcgtgcaa agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca
     4741 tagtagacca gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata
     4801 aagtgggaag ccacgagggg tatgacactt ttaatgaccg tcgtcgctag gagaaagctg
     4861 agtgccaaaa aggtcaggag tctataaggc ttgagatagg acatcaagcg cttcaataca
     4921 gtccattgtt cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg
     4981 gtaggtttgg gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat
     5041 aatttgacca ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg
     5101 ggcagtgata atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata
     5161 ctctgtcttg gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa
     5221 aatcatagcc cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt
     5281 ttcaccaatc agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc
     5341 tagcttagta gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt
     5401 gtctaggatt gaagtcgcaa agagaaactg gtcctgagga acatagccca tgagactgcg
     5461 aaggtctgtc agacgatagt cccgaatatc gtgaccgttt agataaatgg cacccttatc
     5521 cacatcgtat tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc
     5581 aaccaagccc agggtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc
     5641 attttcaaaa gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag
     5701 aggaaactca gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta
     5761 agaaaccttc cctcgctgag tagtattaaa gaggaaaccg atggccagaa gaggccagac
     5821 cagcatatcc aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg
     5881 aaccatcaag gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac
     5941 catagggtca aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc
     6001 attgactgcc tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt
     6061 gatacctgat acggactcct gtaccttgtt attgagttca gaaaaagcag tttgggattc
     6121 gccaaaggcc ttatgagtct ttctccctag gcgactagtc gcataggcca tgaaaggtag
     6181 ggggagaatg gcaacaagag tcatctgcca tgagatgcta aagagcatgg tcaacaaagt
     6241 caccagagcc gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt
     6301 caaggcattg atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata
     6361 aaaggctgac gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa
     6421 gcaataagag gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa
     6481 ggctgcaagt agcaagtaaa ataggctaag aaggaggtcc tgctgggtta attgccccga
     6541 tgtgatggca tcaatgaccc gccccataac cataggagga atgagattga ggacggaaac
     6601 caagaccagg gccacaatcc cgactagata acggcgtttt tctaacttga aaaaccacca
     6661 aagtttttga ataatggaca taaaatccct ttctgattgc aaatggaagc ctgaggccaa
     6721 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc
     6781 tttgaggttg tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga
     6841 cgcggtttga agagattttc gaagagtaca agaccccagg tttttcttat tcataagtta
     6901 cgactgtaac agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc
     6961 cttgtttgct gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat
     7021 ctacttgaat cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgca
     7081 caacgatgag ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa
     7141 tcccttggct acgaagtttg agggctacat ccgatgcaga caaaccaact gaacgacagg
     7201 cttatgggtc aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt
     7261 tcataaagtg aacattgttg ttgaactatt tgagagtcaa gaccagtttg attaaaattt
     7321 cgatataatt aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa
     7381 ttcgactttc tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg
     7441 atctcccacc ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga
     7501 cttgtctact cccaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt
     7561 attgttagaa acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata
     7621 aggctagagc tgaaatcatg accaactcac gaaattcggt tcccttttta tcatggtatg
     7681 ccttaaaagc aaaaaaactg tcacagctaa tagaacaatc ccaaagccaa ccatgatacc
     7741 attttgctga ccgatttggc tgagtacata gtcataagag tagaatttca tgtattttca
     7801 ctccctt
//
LOCUS       DAVMHG010000099         7632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMHG010000099 DAVMHG010000000
VERSION     DAVMHG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.101"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..899
                     /gene="lytB"
                     /locus_tag="VYR08_001594"
     CDS             <1..899
                     /gene="lytB"
                     /locus_tag="VYR08_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV3467099.1"
                     /translation="KNETVDGYQLESDGKWLGGKATNENAAYYQVVPVTANVYDSDGE
                     KLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGH
                     RFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDDFKLENPFLFKDLTEATNYSAE
                     ELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNN
                     FFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASD
                     PYWGEKIASVMMKINEKLGGKD"
     gene            complement(960..2615)
                     /gene="pavA"
                     /locus_tag="VYR08_001595"
     CDS             complement(960..2615)
                     /gene="pavA"
                     /locus_tag="VYR08_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV3467100.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2720..3217
                     /gene="ybeY"
                     /locus_tag="VYR08_001596"
     CDS             2720..3217
                     /gene="ybeY"
                     /locus_tag="VYR08_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV3467101.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3198..3593
                     /locus_tag="VYR08_001597"
     CDS             3198..3593
                     /locus_tag="VYR08_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV3467102.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3610..4509
                     /gene="era"
                     /locus_tag="VYR08_001598"
     CDS             3610..4509
                     /gene="era"
                     /locus_tag="VYR08_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV3467103.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4557..5381
                     /gene="mutM"
                     /locus_tag="VYR08_001599"
     CDS             4557..5381
                     /gene="mutM"
                     /locus_tag="VYR08_001599"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV3467104.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5381..5986
                     /locus_tag="VYR08_001600"
     CDS             5381..5986
                     /locus_tag="VYR08_001600"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV3467105.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5973..7172
                     /locus_tag="VYR08_001601"
     CDS             5973..7172
                     /locus_tag="VYR08_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV3467106.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILAFYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7172..7321
                     /gene="rpmG"
                     /locus_tag="VYR08_001602"
     CDS             7172..7321
                     /gene="rpmG"
                     /locus_tag="VYR08_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3467107.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7361..7594
                     /gene="secG"
                     /locus_tag="VYR08_001603"
     CDS             7361..7594
                     /gene="secG"
                     /locus_tag="VYR08_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV3467108.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
BASE COUNT         2111 a         1445 c         1746 g         2330 t
ORIGIN      
        1 cgaaaaatga gacagtagat ggttatcagc ttgaaagcga tggtaaatgg cttggaggaa
       61 aagctacaaa tgaaaatgct gcttactatc aagtagtgcc tgttacagcc aatgtttatg
      121 attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg ctagataagg
      181 atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca ggctatatga
      241 aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat tatgagagtg
      301 atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta gcttctcatc
      361 tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgcat tttgatgatt
      421 ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac tacagtgctg
      481 aagaattgga taaggtattt agtttgctaa acattaacaa tagccttttg gagaacaagg
      541 gcgctacttt taaggaagcc gaagaacatt accatatcaa tgccctttat ctccttgccc
      601 atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag aataatttct
      661 ttggcattac agcctatgat acgacccctt acctttctgc caagacattt gatgatgtgg
      721 ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatagat aggggaagaa
      781 ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac ccttattggg
      841 gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc aaagattagt
      901 actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt caagtaaaat
      961 caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag ggtcttttgt
     1021 cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt tttgacttca
     1081 atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc agctaactct
     1141 gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac aacatggctt
     1201 ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt aaaggtcaat
     1261 tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc gcttgctaga
     1321 tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg gataaaacct
     1381 gtttgaatca attcttcacg gatttcagcg atttcttcca gtccagcttg gttgaggacg
     1441 gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa atcagtcaag
     1501 tatttgacag cttctttgag tttctgatac cgtttaaaat agcgttgggc attctggttg
     1561 ggagtcagag ccttatcaag cgcaatcatg ataggttggt tggtatagta gttgtctagg
     1621 ataacctggt cttggtcgtt aggcacttgg tggaggaagg ttgtcagcaa ttctcctttt
     1681 tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt tttgagtttg
     1741 tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc ctgctgtttg
     1801 acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga aagatttgca
     1861 aaaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc agtcaagcat
     1921 ggcttggttt cctgattgaa aaaatttcgg aaagcggaaa gtttttcact aaccagtatc
     1981 ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg aagatttttt
     2041 gctgttagtt cttgggtttg caggatttca aagagctttt catccttgat agtaaaagga
     2101 ttgagagatt ctgtacttgg cggagcgata taggtcgatc ctggaagtaa ggtgcggtag
     2161 ctattttgtg aaaaaccgac gtgtttgata acttcgagga ttttatgact gcttttatcg
     2221 accagtagaa tattactgtg tttccccata atttcgataa tcaaggtagc ctggatatgg
     2281 tctccaatct cgtttttatt ggaaactgtc atttccacaa tacggtcatt ttccacttgc
     2341 tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat aaaggtagaa
     2401 ggttgggctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg
     2461 gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac caactcttgt
     2521 tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg caattcctca
     2581 actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg attgtattcc
     2641 atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat aaagccaagt
     2701 aaagagaaac gagaagcaca tgtatattga aatggtagat gaaactggtc aagtttcaaa
     2761 agaaatgttg caacaaaccc aagaaatttt ggaatttgca gcccaaaaat taggaaaaga
     2821 agacaaggag atggcagtca cttttgtgac caatgagcgt agtcatgaac ttaatctgga
     2881 gtaccgtgac accgaccgtc cgacagatgt catcagcctt gagtataaac cagaattgga
     2941 aattgccttt gacgaagagg atttgcttga aaatccagaa ttggcagaga tgatgtctga
     3001 gtttgatgcc tatattgggg aattgttcat ctctatcgat aaggctcatg agcaggccga
     3061 agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg gctttttaca
     3121 tattaacggc tatgatcact acactccgga agaagaagcg gagatgttcg gtttacaaga
     3181 agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa aatcgtgacc
     3241 tgatatccag tttagaattt gccatcacag ggatttttac tgctatcaag gaagaacgaa
     3301 atatgcgaaa acatgcagtg acggctctag tggttatcct tgcaggtttt gtttttcagg
     3361 tgtcacgaat cgaatggctc tttctcctat tgagcatttt cttggtagta gcctttgaga
     3421 ttatcaactc tgctattgaa aatatggtgg atttggccag tcactatcac ttttccatgc
     3481 tggctaaaaa tgccaaggat atggcggctg gcgcggtatt agtggtttct cttttcgcag
     3541 ccttaacagg tgcattgatt tttctcccac gaatctggga tttattattt taaacagtaa
     3601 gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc caatgttggg
     3661 aagtcaacct ttttaaatca cgttatgggg caaaagattg ccatcatgag tgacaaggcg
     3721 cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca aattgtcttt
     3781 atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat ggttgagtct
     3841 gcctacagta cccttcgcga agtggacacc gttcttttca tggtgcctgc tgatgaagcg
     3901 cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt tcctgtgatt
     3961 ttggtggtga ataaaatcga taaggtccat ccagaccagc tcttgtctca gattgatgac
     4021 ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca gggaaataac
     4081 gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca atatttcccg
     4141 tctgatcaaa tcacagacca tccagaacgt ttcttggttt cagaaatggt tcgcgagaaa
     4201 gtcttgcatc taactcgtga agagattccg cattctgtag cagtagttgt tgactctatg
     4261 aaacgagacg aagagacaga caaggttcac atccgtgcaa ccatcatggt tgagcgcgat
     4321 agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg
     4381 gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac ctgggtcaag
     4441 gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta taatgaaaga
     4501 gaatactaag tagaggtagg ctcatgcctg cttcttgttt ttacagaagg agacttatgc
     4561 ctgaattacc tgaggttgaa accgtttgtc gtggcttaga aaaattgatt ataggaaaga
     4621 agatttcgag tatagaaatt cgctacccca agatgattaa gacggatttg gaagagtttc
     4681 aaagggaatt gcctagtcag attatcgagt caatgggacg tcgtggaaaa tatttgcttt
     4741 tttatctgac agacaaggtc ttgatttccc atttgcggat ggagggcaag tatttttact
     4801 atccagacca aggacctgaa cgcaagcatg cccatgtttt ctttcatttt gaagatggtg
     4861 gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat ggaactcttg gtgcctgacc
     4921 ttttagacgc ctactttatt tctaaaaaat taggtcctga accaagcgaa caagattttg
     4981 atttacaggt ctttcaatct gcccttgcca agtccaaaaa gcctatcaaa tcccatctcc
     5041 tagaccagac cttggtagct ggacttggca atatctatgt ggatgaggtt ctctggcgag
     5101 ctcaggttca tccagctaga ccttcccaga ctttgacagc agaagaagcg actgccattc
     5161 atgaccagac cattgctgtt ttgggccagg ctgttgaaaa aggtggctcc accattcgga
     5221 cttataccaa tgcctttggg gaagatggaa gcatgcagga ctttcatcag gtctatgata
     5281 agactggtca agaatgtgta cgctgtggta ccatcattga gaaaattcaa ctaggcggac
     5341 gtggaaccca cttttgtcca aactgtcaaa ggagggactg atgggaaaaa tcatcggaat
     5401 cactggggga attgcctcag gtaagtcaac tgtgacaaat tttctaagac agcaaggctt
     5461 tcaagcagtg gatgccgacg cagtcgttca ccaactacag aaacctggtg gtcgtctgtt
     5521 tgaggctata gtacagcact ttgggcaaga aatcattctt gaaaacggag aactcaatcg
     5581 ccctctccta gctagtctca tcttttcaaa tcctgaagag caaaaatggt ctaatcaaat
     5641 tcaaggggag attatccgtg aggaactggc tactttgaga gaacagttgg ctcagacaga
     5701 agagattttc ttcatggata ttcccctact ttttgagcag gactacagcg attggtttgc
     5761 tgagacttgg ttggtctatg tggaccgaga tgcccaagta gaacgcttaa tgaaaaggga
     5821 ccagttgtcc aaagatgaag ctgagtctcg tctggcagcc cagtggcctt tagaaaaaaa
     5881 gaaagatttg gccagccagg ttcttgataa taatggcaat cagaaccagc ttcttaatca
     5941 agtgcatatc cttcttgagg gaggtaggca agatgacaga gattaactgg aaggataatc
     6001 tgcgtattgc ctggtttggt aattttctga caggagccag tatttctttg gttgtacctt
     6061 ttatgcctat cttcgtggaa aatctaggtg tagggagtca gcaagtcgct ttttatgcag
     6121 gcttagcaat ttctgtctct gctatttccg cggcgctctt ttctcctatt tggggtattc
     6181 ttgctgacaa atacggccga aaacccatga tgattcgggc aggtcttgct atgactatca
     6241 ctatgggagg cttggccttt gtcccaaata tctattggtt aatctttctt cgtttactaa
     6301 acggtgtatt tgcaggtttt gttcctaatg caacggcact gatagccagt caggttccaa
     6361 aggagaaatc aggctctgcc ttaggtactt tgtctacagg cgtagttgca ggtactctaa
     6421 ctggtccctt tattggtggc tttatcgcag aattatttgg cattcgtaca gttttcttac
     6481 tggttggtag ttttctattt ttagctgcta ttttgactat ttgctttatc aaggaagatt
     6541 ttcaaccagt agccaaggaa aaggctattc caacaaagga attatttacc tcggttaaat
     6601 atccctatct tttgctcaat ctctttttaa ccagttttgt catccaattt tcagctcaat
     6661 cgatcggccc tattttggct ttttatgtac gcgacttagg gcagacagag aatcttcttt
     6721 ttgtctctgg tttgattgtg tccagtatgg gcttttccag tatgatgagt gcaggagtca
     6781 tgggcaagct aggtgacaag gtgggcaatc atcgtctctt ggttgtcgcc cagttttatt
     6841 cagtcatcat ctatctcctc tgtgccaatg cctctagccc ccttcaacta ggactctatc
     6901 gtttcctctt tggcttggga accggtgcct tgattcccgg ggttaatgcc ctactcagca
     6961 aaatgactcc caaagccggc atttcgaggg tctttgcctt caatcaggta ttcttttatc
     7021 tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc aggtcaattt ggctaccatg
     7081 ctgtctttta tgcgacaagc ctttgtgttg cctttagttg tctctttaac ctgattcaat
     7141 ttcgaacatt attaaaagta aaggaaatct agtgcgagta aaaattaatc tcaaatgctc
     7201 ctcttgtggc agtatcaatt acctaaccag taaaaattca aaaacccatc cagacaagat
     7261 tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc ctacatcttg aatctaagta
     7321 gattttatgg taaaataata gggattttaa ggagtttgat atgtataacc tattattaac
     7381 cattttatta gtattatctg ttgtgattgt gattgcaatt ttcatgcaac caaccaaaaa
     7441 ccaatccagc aatgtatttg atgccagttc aggtgatttg tttgaacgca gtaaagctcg
     7501 cggttttgaa gctgtaatgc agcgtttgac agggatttta gtctttttct ggctagccat
     7561 tgccttagca ttgacggtat tatcaagtag ataagaaaat aatgggcagg actaggtctt
     7621 tgcctctttt ta
//
LOCUS       DAVMHG010000100         7475 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMHG010000100 DAVMHG010000000
VERSION     DAVMHG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.102"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            120..275
                     /locus_tag="VYR08_001604"
                     /pseudo
     CDS             120..275
                     /locus_tag="VYR08_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            475..3750
                     /gene="rexB"
                     /locus_tag="VYR08_001605"
     CDS             475..3750
                     /gene="rexB"
                     /locus_tag="VYR08_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV3467109.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEVMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILTP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQTEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNHYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3747..7397
                     /gene="addA"
                     /locus_tag="VYR08_001606"
     CDS             3747..7397
                     /gene="addA"
                     /locus_tag="VYR08_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV3467110.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILNQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SKSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADAYNEERKTR
                     FAKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2280 a         1579 c         1584 g         2032 t
ORIGIN      
        1 gattgaaata agatacgaac agcttgatta gggaaatcaa attaatttct agaaatattt
       61 tagcatccac gatgtactat tatagtttca atttactata gttcaatcat tctatcaaga
      121 atattgaata tataaaggca cgtaatatta aaacttcttc tgatatcgaa ttagagtccg
      181 atgttgatgg cgataaatca gacaatctac ccgtcgacat taagatactg ggacaacaca
      241 ttgaagtgta ttccgtacct aagaaacaat cataaaactc aaagtcttgc taccgaggct
      301 tttttattat gtgattttca gtagccagac ttattctatc taatacaagt aaagctaagt
      361 atttaagatt tagcagatga tgaagaaaag aaaggatggc ctaaatattt taacagattt
      421 acgaggctaa tcatggtata atatagggtg atgaaaaatt ccaacgaggc tgagatgaaa
      481 ttactttata ctgatattcg aacttctttg acagaaattc taacaagaga ggcagaagag
      541 ctagttgcag ctggcaagcg ggtcttctac attgccccca actctctttc ttttgaaaag
      601 gaacgcgccg tgctggaatg cttgtcccag caggcttctt tttcgattac cgtcacgcgc
      661 tttgctcaaa tggctcgtta tctggtcttg aatgatttac cagttaaaac tactcttgat
      721 gatatcggtc ttgggttggc cttttacaaa tgccttgccg aactcgatcc caaggacttg
      781 cgtgtttatg gcgctattaa gcaggatcct caattgatcc agcagttaat tgagctttac
      841 catgagatga ccaaatctca gatgagtttt ttggacttgg agaatttaac agatgaggat
      901 aagagggcgg atttactctt gatttttgag aaagtaacag cctatcttaa tcaaggtcag
      961 ttagcccagg gaagtcagtt gtcccatttg attgaggcta ttgagaatga caaggtaagt
     1021 agtgatttta atcaaatcac cttggtcatt gacggcttta ctcgtttttc tgctgaggaa
     1081 gagcgggttg tggacttact tcacggcaaa ggtgttgaga ttgttatcgg ggcttatgct
     1141 agtaagaaag cctataccag tccttttagc gagggcaatc tctaccaagc cagcgtaaaa
     1201 tttctccatc atctggcttc taaataccaa acgcctgctc aggactgttc tcaaactcat
     1261 gagaagatgg atagttttga caaggcctct cgtttgttgg agtcttctta tgacttttca
     1321 gaactcgctt tggatgtcga tgagaaagac cgtgaaaatt tacaaatctg gtcttgtttg
     1381 acgcaaaagg aggagttgga gctagtagcc cgtagcattc gtcagaaatt acatgagaac
     1441 tcagacctga gctacaagca ttttcgtatt ctcttggggg atgtagcttc ttaccagtta
     1501 tctctcaaaa ccatttttga ccagtatcag attccttttt atcttggtag aagcgaagtc
     1561 atggctcatc atcccttgac tcagtttgtc gagtctattt tagctttaaa acgttaccgt
     1621 tttcgtcagg aggatttgat taatcttctt agaactgatt tgtatactga cctcagtcag
     1681 tctgatattg atgcttttga gcaatatatc cgctatcttg gtatcaatgg cttgccagcc
     1741 tttcagcaaa ccttcaccaa atcccaccat ggaaaattta atcttgagcg tttgaatgtc
     1801 ctccgcctga gaattttaac acctcttgaa accctctttg ccagccgaaa acaaaaggct
     1861 gaaaacctcc tacaaaaatg gagtgtcttt ctaaaagaag gagctgtgac taagcagtta
     1921 caagatttga caaccacttt ggaagctgta gaacaggaaa gacaaaccga agtttggaag
     1981 gctttctgcc atgttttaga acaatttgcg actgtttttg ctggttcaca ggttagtctg
     2041 gaagacttcc tagccttgct ccattctgga atgagtttgt cccaataccg taccattcca
     2101 gcaacagtgg acactgttct ggtgcagagt tacgatttga ttgcaccatt gactgctgac
     2161 tttgtctatg ctattggact aactcaggac aatttaccaa aaatttctca aaacaccagt
     2221 cttctgacag atgaagaaag gcaaaaccta aaccaaacga ccgaagaagg cgttcaatta
     2281 ctgattgcca gcagtgaaaa tctcaagaaa aatcactaca ctatgctttc cttggtcaat
     2341 tctgctcgta agcagttgtt cttgtcggct ccaagccttt ttaacgaaag tgaaagtaag
     2401 gaatctgcct atcttcaaga gttgatccat tttggattta ggcggagaga gaagaggatg
     2461 aatcacaaag gactgtctaa ggaggatatg gggtcctatc acagtctttt gtctagtctg
     2521 gttgcctatc accagcaggg tgagatgagc gatactgagc aagatttgac ttttgtcaag
     2581 gttctgtcgc gtgtcatagg taaaaaacta gatcagcaag gtctggaaaa tccagctatc
     2641 ccaaccagtc caagcagcaa gaccttagcc aaggacacct tgcaagctct ctatcctgcc
     2701 aaacaggagt tttacctgtc tacgtcgggt ttgacagagt tttatcgcaa tgaatacagt
     2761 tatttcctac gctacgtttt aggcttgcag gaggaattac gtttgcatcc tgatgcccgt
     2821 agtcacggga atttcttgca tcgtatcttt gaacgcgcct tacagttgcc taatgaagat
     2881 tcctttgacc aacgtctaga acaagctatt caagaaacca gtcaagaacg cgaatttgaa
     2941 gctatttatc aagaaagttt ggaagcccag tttaccaagg aagttttgct tgatgttgca
     3001 cggacgactg gacatattct ccgacacaat ccagccatcg aaaccatcaa agaagaagca
     3061 aattttggtg gaaaagacca agcctttatt caattagaca atggacgcag tgtctttgta
     3121 cgaggcaagg tggaccggat tgaccgtttg aaagctaatg gagcgatagg agtagtagac
     3181 tacaaatcca gtctgactca gttccagttt cctcatttct ttaatgggct caattctcag
     3241 ttaccaacct atcttgctgc cctaaaaaga gaaggggagc agaacttttt cggcgccatg
     3301 tacttggaaa tggctgaacc tgtccaatct ctgatggcgg taaaaagtct ggcaggagca
     3361 gtggtagaag ccagcaaatc tatgaaatac caagggctct tcttggaaaa agaaagcagt
     3421 tatttaggcg aattttataa caaaaacaag gctaatcaac tgacagatga ggaatttcag
     3481 ctcctactgg actacaatgc ctatctttac aagaaagctg ctgagaagat tttagcaggc
     3541 cggttcgcca tcaatcctta tactgaaaat ggcagaagca ttgccccata cgtccagcaa
     3601 catcaggcta ttacaggctt tgaagccaat taccatctgg gccaagcccg tttcctaaaa
     3661 aagttggacc tagctgatgg caagcgtctg gtcggagaaa aactcaagca agcttggttt
     3721 gaaaaaataa gagaggagtt aaatcgatga agcttattcc ctttttaagt gaggaggaga
     3781 ttcaaaaact gcaagaagca gaagcaaatt cgagcaagga acaaaagaaa actgccgagc
     3841 aaatcgaagc tatctacact tctggccaga atatcctggt ctcagcatcg gctggttctg
     3901 gaaagacctt tgtcatggca gagcgcattc tgaaccaatt ggcgcgtggt gtcgaaattt
     3961 ctcaactctt tatctcaacc tttaccgtca aggctgcaac tgaacttaaa gaacgtttag
     4021 agaaaaaaat cagcaagaaa atccaagaaa cagatgatgt cgagctcaaa caacacttgg
     4081 gtcgccagtt ggcagaccta cccaacgctg ccatcggaac catggactct ttcacacaaa
     4141 aattccttgg caaacatggt tatctgcttg atattgcacc taatttccgt attttacaaa
     4201 accaaagcga gcaacttctt ctcgaaaacg aagtctttca tgaggtcttt gaagcgcatt
     4261 accaaggtaa acagaaagag acctttagtc atttgctgaa aaactttgct ggacgtggca
     4321 aggacgaacg gggtctgcgc cagcaggtct ataaaatcta tgacttcctc caatccacca
     4381 gtaatcctca aaagtggctg agtaaatctt tcctcaaagg ttttgagaaa gctgatttta
     4441 ccagtgaaaa agaaaaactg actgagcaaa tcaaacaagc cctttgggat ttggaaagct
     4501 ttttccgtta ccatctggat aacgatgcca aggagtttgc aaaggctgcc tatttagaaa
     4561 atgttcagtt aattctggat gaaattggct ccctaaatca ggagtccgat agtcaggctt
     4621 atcaggcagt gcttgcgcat gttgtcgcca tctctaagga gaaaaacggt cgagctctga
     4681 ctaatgccag ccgtaaggct gatttgaagc ccctggctga tgcctacaac gaagagagaa
     4741 agacccggtt tgctaaacta ggacaattat cagaccagat aaccattctc gactatcaag
     4801 aacgttatca tggagacact tggaaactag ctaaaacctt ccaatctttc atgagcgatt
     4861 ttgtggaggc ttatcgtcag agaaaacgac aggaaaatgc ctttgaattc gctgatatca
     4921 gccattacac cattgagatt ttagagaatt tcccacaagt tcgtgagtct tatcaggagc
     4981 gcttccatga agtcatggtc gatgagtatc aggataccaa ccatattcaa gaacggatgc
     5041 tggaattgtt gtctaatggc cacaatcgct ttatggtggg agatatcaag caatccatct
     5101 atcgtttccg tcaggcagac ccgcagattt tcaatgagaa attccaacgc tatgcgcaaa
     5161 atccccaaga aggcaagctc attctcctca aggaaaattt ccgtagtagt tcagaagtgc
     5221 tgtcagcaac caatgatgtc tttgaacgtc tcatggacca agaggtcggc gaaatcaact
     5281 atgataacat gcaccagctt gtttttgcca ataccaaact gactcccaat ccagacaaca
     5341 aggccgaatt tctcctctac gacaaggacg atacaggtga ggaagaagag agtcaaacag
     5401 aaacgaaact aacaggtgaa atgcgcttag ttatcaagga gatccttaaa cttcatcagg
     5461 aaaaaggtgt tgcctttaag gaaattgccc ttctgacctc tagtcgtaat cgtaatgacc
     5521 aaattctact tgccctgtct gagtacggga ttcctgtcaa aactgacgga gagcaaaaca
     5581 attatctcca atccctagaa gtgcaagtca tgctagacac tcttcgtgtc attcacaatc
     5641 ccctgcaaga ctacgccttg gttgccctta tgaagtctcc aatgtttggt tttgatgagg
     5701 atgagctagc acgtttgtcc cttcagaaag cagaggataa agcccacgaa aatctctatg
     5761 agaaactggt caatgcacaa aaaaaggcaa gtagtcaaaa aggcttgatt cacacagctc
     5821 tagctgaaaa actaaagcaa ttcatggata tcctagcttc ttggcgcttg tatgccaaac
     5881 cccactctct ctatgacttg atttggaaga tttacaacga ccgtttttat tatgactatg
     5941 ttggggcttt gccgaatggt cctgctaggc aggccaatct ctatgcccta gcactgcgtg
     6001 ctgatcaatt tgaaaagagc aatttcaaag gtttgtcgcg ttttattcgt atgattgacc
     6061 aagtcttaga agcccagcac gatttggcaa gcgtggccgt cgcaccgcca aaagatgcag
     6121 tagagctcat gaccatccac aagagtaaag ggctggagtt tccttacgtc tttatcctca
     6181 atatggatca agatttcaac aagcaagact ctatgtcaga agtcattctc agtcgtcaga
     6241 atggtcttgg tgtcaaatat attgccaaga tggagacagg ggcagtagaa gaccactatc
     6301 ctaaaaccat caaactctcc attcctagtc tgacctatag gcagaacgaa gaggaattac
     6361 agctagcaag ctattctgag cagatgcgtt tgctgtatgt tgctatgacg cgggctgaga
     6421 aaaagctcta tcttgtcggc aagggttctc gtgaaaagct ggaagccaag caatacccag
     6481 cagccaaaaa tgggaaacta aatagcaata ctagactgca agcacggaat ttccaagatt
     6541 ggctttgggc tatcagtaaa gtgtttacta aggacaagct caactttagt tatcgtttta
     6601 ttgacgaaga tcagttgacc agagaagcta tcggagagtt ggaaaccaag agtcctctcc
     6661 aagatagctc ccaagcagac aatcgtcagt cagataccat caaagaagct ctggaaatgc
     6721 tgaaggaggt ggaagtttat aatactcttc accgcgcagc tattgaactt cctagtgttc
     6781 aaaccccaag tcaaatcaag aaattctacg aaccagttat ggatatggaa ggtgtcgaga
     6841 ttgctggtca aggtcagtca gtaggcaaga aaatcagctt cgatttgcca gatttttcaa
     6901 ccaaagaaaa ggtaactgga gctgagattg gtagtgctac tcacgaactc atgcagagaa
     6961 ttgacctcag ccagcaacta acccttgcta gcctaacaga aacactcaaa caagttcaaa
     7021 ctagccaagc tgtcagagac aagatcaatc ttgataaaat tcttgctttc tttgacacag
     7081 tactcggtca ggaaattctt gctaataccg accatctcta tcgcgagcaa cctttctcca
     7141 tgctcaaacg agaccaaaag agtcaggaag actttgttgt ccgtggtatc cttgatggct
     7201 atctgcttta cgaaaacaaa attgttctgt tcgactacaa gacagaccgc tatgatgaac
     7261 caagtcaact cgtagaccgc tatcgtggtc agttagctct atacgaagag gctttatcac
     7321 gagcctattc gattgaaaat attgaaaaat acttgatttt actcggtaaa gacgaggttc
     7381 aagttgtaaa agtataactt atactcttcg aaaatctctt caaaccacgt cagcttcgcc
     7441 ttgctgtata tatggttact gacttcgtca gttct
//
LOCUS       DAVMHG010000101         7382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMHG010000101 DAVMHG010000000
VERSION     DAVMHG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.103"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(85..846)
                     /locus_tag="VYR08_001607"
     CDS             complement(85..846)
                     /locus_tag="VYR08_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV3467111.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(888..1514)
                     /locus_tag="VYR08_001608"
     CDS             complement(888..1514)
                     /locus_tag="VYR08_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV3467112.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(1530..1847)
                     /locus_tag="VYR08_001609"
     CDS             complement(1530..1847)
                     /locus_tag="VYR08_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV3467113.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(1844..2143)
                     /locus_tag="VYR08_001610"
     CDS             complement(1844..2143)
                     /locus_tag="VYR08_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV3467114.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2428..2619)
                     /locus_tag="VYR08_001611"
     CDS             complement(2428..2619)
                     /locus_tag="VYR08_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV3467115.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(2780..3454)
                     /locus_tag="VYR08_001612"
     CDS             complement(2780..3454)
                     /locus_tag="VYR08_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467116.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGISSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(3460..3906)
                     /locus_tag="VYR08_001613"
     CDS             complement(3460..3906)
                     /locus_tag="VYR08_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467117.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(4020..4523)
                     /locus_tag="VYR08_001614"
     CDS             complement(4020..4523)
                     /locus_tag="VYR08_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV3467118.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(4520..4882)
                     /locus_tag="VYR08_001615"
     CDS             complement(4520..4882)
                     /locus_tag="VYR08_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV3467119.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(4948..6813)
                     /locus_tag="VYR08_001616"
                     /pseudo
     CDS             complement(4948..6813)
                     /locus_tag="VYR08_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(6845..7294)
                     /locus_tag="VYR08_001617"
     CDS             complement(6845..7294)
                     /locus_tag="VYR08_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3467120.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
BASE COUNT         2226 a         1748 c         1335 g         2073 t
ORIGIN      
        1 tatacatatt ttcttcctcc tacttatcta ttcgtaggaa atcaaaaaaa gttacagaaa
       61 tttgtaactt ttcgagaaaa ttttttattt tttatgaacc atgaaacctg tcgcctgttg
      121 attggccata atggtcatat ctgtaatctg aacacgacga ggttgactag tcacatagac
      181 tactgtatct gcaatatcct gagcttgcaa agcttctatt ccttggtaaa cggacgcagc
      241 tcgttcttta tcaccatgaa aacgcactgt agaaaaatct gtttcgacaa ttccaggctg
      301 aatggttgtc accttgatat ccgttgcgat ggtatcaatt cgcagtccat ccgaaaaggt
      361 cttaaccgca gccttggtag ctgagtaaac agcggcacca gcataggcgt agattcctgc
      421 ggttgacccc atattgataa tatgaccttg attgactttt atcattgctg gcaagaaaca
      481 gcgagtaacc gccatcagac ctttaacatt ggtatccaac atggtcaaca tatccaactc
      541 ctcatagtct tgatagggag ccaagtcaag agccagtccg gcgttgttga ccaagatatc
      601 aatcctccct atcgtttcta gaatatcaga gcaaacagtc ttcaccatga ccatatccgt
      661 cacatctagt ggaaaagtcc agacggtttg atttggaaag cttactgcaa actccgactt
      721 gaggatttct aatctgtcta tccgtcgtcc tgttagaacg acatcctcac cctgctccag
      781 ataagcacgc gcaatcgctt caccgattcc tgaggttgct cccgtaatca caacattttt
      841 tgccatctta tttccttcta gctggtctat cagatattaa caacttctta ggcagtccag
      901 tgtttagctg agtcaaatgg tgttccgaca acttggtctt ctgataattc aagcacacca
      961 cgtttttgtg gagcatttgg cagatgcaat tcacgaggac tgcacatcat accaaaactc
     1021 ttttcaccac gaagttcacc tgggaaaatg agattccctt ttggcatcat agctccagga
     1081 agcgcgacaa tggttttcaa ccccacacgc gcattgggag ctcctgcaac gatttgtaca
     1141 gtcttatcac ttgcgactgc aacttggcag atgttgaggt ggtcactatc tggatgggct
     1201 accatctcaa caatttcacc gacaacaaac ttaggttcct tatcattaac aatttcttct
     1261 gtaaaacctt ccgcctgcaa ctcttggttc aaacgagcga cttgctcatc tgtcaaaaag
     1321 acttgaccgc gctctgcagt ttcaaataaa cttgaaactt cgaaaatatt ccaagccact
     1381 gtttccccat tttctttgag gaaaacacgg gctaccttgc ctttgcgttc cacattcagt
     1441 ttggcatctc cgctattttt cacgatgacc ataaggacat caccgacatg ttctttgtta
     1501 tacgtaaaaa tcattgtttc ctttttctcc tatttcaatc ctgctaaaaa atcgttaatt
     1561 tgctccttac ttttacggtc gcgattgaca aaacgaccga tttccttgtc cttttctaga
     1621 acaacaaggc taggaattcc gtaaacatcc cagagtttgg ccaaatccat atactgatct
     1681 cggtccattc gaataaaggt gaactctgga ttggtctctt caatctctgg taaggcagga
     1741 taaatataac gacaatcgcc acaccagtct gccacaaaaa ggaagacctt cttgcccgct
     1801 ttttccacta aagatgctaa ttcttctaaa cttgctggct gtatcataag acttcctcct
     1861 catagactag gtcttcattt tcatagacaa aggtataatg acggccatcc tcaaaaatga
     1921 cgccaccaac caagctctcc agactgcttt cgtaaacttg aacataaagg gtcgcaattt
     1981 cccccatgtc ggaaaaatgg tctcgcacaa tctctgtcaa ctcttcctga gtcttcatga
     2041 gcttacggtc atctgcaact tttttcgtag caagagcaag gcttccgata cctagcagag
     2101 ccaagcctgc catccacatt tttttagctt tcataccatt cattttaaca caaaaaaggc
     2161 ttcaggacaa atgaggaagc agcagaaaag caagtaaaaa gcctcttcct ttaaggaaaa
     2221 ggacttctta tactaagagc aaactagaaa gctagccgca ggctgctcaa aacacggttt
     2281 tgaggttgta gataaggcga cgttgacgtg gtttgaagag attttcaaag agtattattc
     2341 ttattgccag gcacctaagt tgccaacgta gtaaccatca ggtgtgtagg tattgcgaag
     2401 catcttacct gatgaagcca gataatacta cttgccattg tctttgaccc aatcattcgc
     2461 aatcatggaa ccagaagaac ttacataata ccattctccc ttgtcataaa cccaagtact
     2521 gactttcatg gttcctgagc aattaaaggc aaaaaaactg tccaatatca ttcgtttttt
     2581 aaaagcattc gacactacat catctacgct gtagtacata gatacatcaa acgtaaaagc
     2641 caaaatatcg aaaaatatac aacttttaaa ggcagcattt cattttttaa agtctaacaa
     2701 ccatagaact gaaatcagag caaggcatca atttcctact ctatagtacg cctattttga
     2761 tggttgaagt gaagattttt tatttaacgt agacaatttc caacttccca aaagccacat
     2821 ctccacggat aatcaaggtt ttgctggttg gggctacagg agcgtcagcc ttagctgcac
     2881 caaaggaggt ttctactttc aaatctacac gccagtgttg tggaacatag acggttgcat
     2941 ttccgaaggc tacttcaata tttaaagttg caaaatcacc tagcatctct gcattatcat
     3001 agtagatttt agcatcccca aaagcgactt ccacttggtc atctacgaga tcttgatctt
     3061 gcttatagaa ggtcccgcta ccaaaagcga cttccttatc cgtgacgact gtttttttcc
     3121 cattgtacca ccattttttt tcattccaga acttacttga atgcgtcaga taaccaacac
     3181 caagtaccac caagatgcta gcccaaataa gagaatgatt ggtaactggt aacaagtcat
     3241 aagcgtaatt tgcaatgatg agcgccagta aacctgtaaa aactgccgaa gtgagatgac
     3301 gtctaaggat ggactcaatg gacttataag caaaaaaaac aatacctagt aaaggccata
     3361 ttttaccatc caaagaagaa attccaaaat tcccttgcag caagatccaa gctgctaaaa
     3421 ccaataaaac aataccaaat gctttctttt tcatgttttt tacctcatgt ttcttagatt
     3481 ttcttttagg agggattggt aatgccgtga gacatgaacc tccttgtgcg tctgataaaa
     3541 ggaaatggtg cccgttcctg aaaaggactt gtccactgag taaatctgac ggatgtttgc
     3601 gatcgttgac ttggaaactc gattaaaata gcgaggcaag atagactcca actcatagag
     3661 cttgaggcga acctcataag cttccttctg gttatgagcg tagatcttgc tcccttctgt
     3721 ttcaaagaag agaatttctg acaggtctag ataatattca cctgtcccct tgtaaaaagt
     3781 caacctcgga gcctttgact cttgcaagag ccgttgcaag tctgcaattt catctgtcaa
     3841 agtgggggtc ttgatgacaa tttcagtctc ctctaaattg ccgtcaatct caattcgtaa
     3901 cttcatcaca tctcctttct gactctacta tatcaaagct aagttaggaa tacaagggca
     3961 aaaaagcaag tggtcaattt gaagcagtaa gtggttgtag gactagctta acttacatgt
     4021 cagaatagga agaaaaggaa tccccacacc aaaccacaag catagccaac caagacatcc
     4081 ttgggataat gaacaccccc taaaaccctc accaacccca gcaaagctga aaagatcaag
     4141 catactaagc caataggtag actagcatgc agacaggcca tggagatgat agttgccgaa
     4201 aagacatggc gactgggcat cgaccgtccc gaactatcct tatcaagaag gggacagata
     4261 tcccaagttt cataaggtct cgggtgattg attttcttac gaaacaggga caaggtcaca
     4321 aaacctgatg caggaataag cacataaacc ccaacttgct gaccaagtcc tagttgcagg
     4381 taagtggtga ctagcaagat cagatagacc aggggcatgg cgactgtcat caaacgattg
     4441 aaactgcgta ataggaataa aagaattgga cgactagtta gtctggagct gatatttcga
     4501 taccattctt gataattttt catttatgct tctcatactt ttctagaact tgttgagatg
     4561 ttttcccctt ggcaagccaa tccactagct tgtccaagcg acgtatttcc tgcatcagag
     4621 gatcatcaat ttcctcaata cggacaccgc aaatcttccc tgtaattgca attcgttcag
     4681 ggacataata tggtgcttgt ctaaaaaaat ctccgtaagt aacatcccta tccagacaag
     4741 caagaacatc actcacttca taccctgtca accaactggt cacttggtga actgactctg
     4801 cctttccacc ctttcgctca acttttgcaa tcaaggctaa gtagacagca gcaaatttca
     4861 tattgtataa tttctgactc atacttctct ctttctatgt gtactagtat aaataagaaa
     4921 aagaaggccg tcaagccttc ttttgattta ttcttctgct tcatcttctg taaattgact
     4981 attgtacaag tcagcgtaga agccaccttg cgccatcagt tcctcatagt tgccttgctc
     5041 gatgatattt ccatctttca tgaccaagat caagtctgca tttcggatgg ttgacaagcg
     5101 gtgggcaatg acaaaggatg tgcgtcattc catcaaacgg tccatggctt tttggatcaa
     5161 ttcctctgtc cgtgtgtcaa cagaagaagt cgcctcatcc aaaatcaaaa gcggtgcatc
     5221 cttaagaagg gcacgagcaa tagtcaatag ttgtttttgt cctacagaca aggtcacggt
     5281 gtcatccaag atggtatcat agccatctgg caaggtcata ataaagtggt gaattcccac
     5341 agccttacta gcttccatca ttcgttcatc actaatccct atttgattat agatgagatt
     5401 gtctcgaata gttccttcaa agagccaggt atcctgcaag accattgaaa aggcatcatg
     5461 cacttctgaa cgcgtcatag ccttggtatc cacaccatca atgcgaatac ttcccttatc
     5521 aatctcatag aatttcatca aaagattgac aatggttgtc ttaccagccc cagtcggccc
     5581 aacaatggca accttttgac cagcatgagc tgtcgcagag aagtcatagt cttgaacatt
     5641 gacaccgtcc accagaattt ctcctgctga cacgtcgtag aaacgtggaa tcagattgac
     5701 cagagttgat ttaccagaac ctgttgaccc aataaaggcc actgtttgac cagtttctgc
     5761 tttaaagcta acatgttcaa taactgcctc cgaatttgcc gcatagcgga aggtcacatc
     5821 cttaaactcg acctgacctt tgaagttttc atcagtcagc tgcacttgaa cagggttttg
     5881 gatagaagaa tgcaaatcta aaacttgatt aatccgctta gcagagacca tagttcgggg
     5941 aagaacgatg aagagtgctc ccatgagaag gaagcccatg acaacctaca tggcataaga
     6001 catgaaaaca atcatgtcac taaagagagg cagacgcgct atcggagcag cgtcgttaat
     6061 cacataggcc ccaatccagt aaatcgctac actcaaacca cttgaaatcc ccatcatgat
     6121 aggattcaaa atagccataa gacggttgac aaacaaattc aaacgggtca attcatcatt
     6181 tactgctgca aatttttcat tttgataatc ctctgcattg taggcacgaa cgacacgaat
     6241 acctgttaaa ctctcacgag tgatactgtt cagtttatct atcagcccct gaatcaagga
     6301 ctgttttgga aagtctagcg tcatcaaaac ggtcgtcatc aggacgttga caatcactgc
     6361 cacaagtacg gcccaaagcc agtattctga atgacctaaa atcttcccaa tagcccaaat
     6421 agccataatt gaaccacgcg ttaccacttg caagcccata gtaatcaaca tttgaacttg
     6481 agtaatgtca ttggtagtac gcgtcaagag gctaggaatt gaaaatttct taatctctgt
     6541 ctgcgagtaa tccaaaactc ggttaaaaat atcacttctc agcctactag tataagaagc
     6601 cgccactcgg gatgcaaaaa atccaactgc aactacggac aagaaggcaa gaaagagcat
     6661 tcccatcatc atgcttgccg gctgccacaa ctcatctaaa ttagttcctt gactacctag
     6721 caaatccgta attttcgaga tataggtcgg cacttccaac tctagataga ccgaaaagca
     6781 agtaaagaga atggctagta aaatcatccc cattcttttc tactaattct tttggctaat
     6841 ttctttattc tctcctccta ttcccttgat attttgcctg tagttgaccg agaaccttct
     6901 caaaaatcag taattcatct tcatcaatgt cttccatcaa ctgcttgtct atgcgttcaa
     6961 aaaaagcctt aacctgttgc atctgagaac gtgctttgtc cgtcagacga acaaacttag
     7021 cccgcttatc aacaggactc gcctccaatt ccaccaaacc attttgcact atacgcttaa
     7081 ccagattact agcaacagac ttggtaatat tgagttcctg atcgatatct ttaatcaaga
     7141 ccaagtcttg gtttttctcg cgattatcca aaaaatgcac aacctgacct tgcggcccac
     7201 ccataaattc aatgccgcaa cgtttggctt ccttttgcac catcaggtga atttgatgac
     7261 caaaacgctt aaagactaac atcggtttac ccataatctc ccccttctaa ataaaaatag
     7321 ttctctggag aataattaaa tttctatgag aactattttc ttgattaaaa aaatcccaag
     7381 tg
//
LOCUS       DAVMHG010000102         7363 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMHG010000102 DAVMHG010000000
VERSION     DAVMHG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.104"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..109
                     /locus_tag="VYR08_001618"
                     /pseudo
     CDS             1..109
                     /locus_tag="VYR08_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(246..1214)
                     /locus_tag="VYR08_001619"
     CDS             complement(246..1214)
                     /locus_tag="VYR08_001619"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV3467121.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALSEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1359..1901)
                     /locus_tag="VYR08_001620"
     CDS             complement(1359..1901)
                     /locus_tag="VYR08_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV3467122.1"
                     /translation="MDQLLGVLFPDSTFLNAFESAIVAPLVEEPLKLLPLVFVLALIP
                     VRKLKSLFLLGIASGLGFQMIEDIGYIRTDLPEGFDFTISRILERIISGIASHWTFSG
                     LAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAIPVVTAI
                     ALYGFYHAYCFVEKHNELMT"
     gene            complement(1905..2204)
                     /locus_tag="VYR08_001621"
     CDS             complement(1905..2204)
                     /locus_tag="VYR08_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467123.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFHIGKKWELPKKVLILSLLGAICSFTSLLLFGIYNHRRKSSKV"
     gene            complement(2229..2726)
                     /locus_tag="VYR08_001622"
     CDS             complement(2229..2726)
                     /locus_tag="VYR08_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV3467124.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(2799..4160)
                     /gene="radA"
                     /locus_tag="VYR08_001623"
     CDS             complement(2799..4160)
                     /gene="radA"
                     /locus_tag="VYR08_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV3467125.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4174..4689)
                     /locus_tag="VYR08_001624"
     CDS             complement(4174..4689)
                     /locus_tag="VYR08_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV3467126.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGEMLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4691..5134)
                     /locus_tag="VYR08_001625"
     CDS             complement(4691..5134)
                     /locus_tag="VYR08_001625"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV3467127.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(5194..5287)
                     /gene="ffs"
                     /locus_tag="VYR08_001626"
     ncRNA           complement(5194..5287)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYR08_001626"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5320..5787)
                     /locus_tag="VYR08_001627"
     CDS             complement(5320..5787)
                     /locus_tag="VYR08_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV3467128.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5988..7274)
                     /locus_tag="VYR08_001628"
     CDS             complement(5988..7274)
                     /locus_tag="VYR08_001628"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV3467129.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         2284 a         1831 c         1221 g         2027 t
ORIGIN      
        1 accttctaaa attccttatc ttgaaacgac tggtatacct actagaattc tccttagaaa
       61 gcgtcgattc aagtgctatc actgttcaaa aatgatggtc gctgaaactt ctgatgacgt
      121 acagtcatat ttcttctctt tttattatat cacagtttta aatctagctt tactagattc
      181 accgctacta tctatttatt cggaaaaaag acgaaaaaac ctgagaatca tctcaggctt
      241 ggtcattaaa tttttttctc aatatcgaaa agtggagaaa gtggtcgttt ttcatgaata
      301 cgtacgatag catcccctag gagatgagcg attgaaatct gctcaatctt atcaatcaaa
      361 cgctcttctg gcagatagat ggtatccaaa acaaccaatt tcttaatagc tgatttttgg
      421 atattgtccg tagcaggacc agaaagaact gggtgcgtac agcttgcata gacttcaaca
      481 gcaccagctt ccgaaagagc atctgccgca tgacaaatcg ttccagcggt atcaatcata
      541 tcatcaatca agatacaagt cttgccttca accttaccga tgatgttcat aacttcacta
      601 gtattcatct tatcaacgct acgacgttta tcaataatag cgatagatgt tttcaaaaat
      661 tctgccaact tacgagcacg agtcacccct ccatggtccg ggctgacaac cacatagtca
      721 gaaccaacca taccacgacg ctcaaaataa tctgcaatca gaggagcacc catcaaatga
      781 tccacaggaa tatcaaagaa tccttgaatt tgcgcagcat gcaagtcgat ggtcaataaa
      841 cgatccactc cagctacttc aagcatattt gcgacaagtt ttgaagtgat tggctcacgc
      901 gctctcgcct ttctatcctg acgtgcatac ccatagtaag gcatgacaac attgacagat
      961 tctgcactcg cacgcttcaa agcatctacc ataatcaaaa tttcaagcag attgtcattt
     1021 acaggcgaac tagttgattg taggataaag acgtgtttcc cacggattga ttcttcaatg
     1081 ttgacctgaa tctctccatc tgaaaattgg cgaacacttg atttccccaa ctctatccca
     1141 atctcctgcg ctacacgttc tgccaattct ttattagaag aaagggcaaa cagctttaaa
     1201 tcagaaaaag acatgatttc ctccggtata tatgtataac ttgtgctttt cacaagattt
     1261 tccatctacc attgtagcgc tttttgcact atttttcaat caaaaataaa agaagggcac
     1321 catatttgta cccttgcatc attcttttga aaaatattct aggtcatcaa ctcattgtgt
     1381 ttctcaacaa agcaataagc atgataaaaa ccatagagag caatagccgt aaccactgga
     1441 atcgctaaag gcaactctgt ttccaactcc acaaaaggag agttaaacaa gaagtgagtt
     1501 cccaaggcta aacctagaaa aataaggccc tgtttcttgc caaccttctg tcctttatag
     1561 gctctgtaaa gcaagtaaac acctactaca gctagacctg aaaaagtcca gtgagaggca
     1621 attcctgaga tgatacgctc taaaattcgc gaaatagtaa agtcaaagcc ctctggcaaa
     1681 tccgtacgaa tataaccaat atcctcaatc atttggaatc ccaaaccgga agcaattcca
     1741 agtaaaaaca aagattttaa ttttcgcaca ggaatcaaag ccaaaacaaa aacaagtggc
     1801 aataatttca agggttcttc taccaaagga gccacaatag cactttcaaa ggcatttaaa
     1861 aatgtactat ctgggaaaag aacccccagt aactggtcga ttttttatac cttagatgac
     1921 tttcgtctgt gattatagat tccaaatagt aagagagaag taaaggaaca gattgctccc
     1981 aataaagaca gaatcaaaac cttctttggc aattcccatt ttttcccaat atggaagaga
     2041 aaataaagag ccggaatcat gtaaaagaga gctagaaaga tagaaactcc cattagtcca
     2101 tattccgcac ctgacctcga accgtccgta tagtagatgg tttcatactg taaaccaata
     2161 catagcaata aaataaaaat aaataaaata ctgcttttct tcatacactt tctttctaaa
     2221 tgaagtattt ataattctac gactgccata cttcctgtat caacattgta aatggcacca
     2281 gagataatga catcgtctgg tattagggga gactcgataa gcagttgcat atcctcgcgt
     2341 acactctctt ctatatcttg gaagggcaag aagtcctggt ctgacacatc tacacctaat
     2401 tcctctttta aatactcctg aaaaggttca ttttcaaagg tctgagcacc acagtctgta
     2461 tggtgcaata ccacaatctc tcttgtcccc atttgttgct gggaaataac tagcgaacga
     2521 atcatgtctt cagtcactcg accacctgca ttccgcaaga tatgagcatc cccaagtgcc
     2581 aagcccagag cttgcgcaac gtgcagacga gagtccatac aggtcacaat agctactctt
     2641 gttttgggtt taagtggcag atttaactgc ccatgtaggg caacataagc ctgattagct
     2701 tgcataaact gttcaaaata cgacacgatt ccctccttga aaatttgata gtcaaatatt
     2761 tctcctatct tatcattttt aagagaattt gtcacggatt atgcaaagac ctttttcaaa
     2821 acttcctgaa tcgttgtcac gccaatgacc tgaatttcct taggcagagt gattcctgtc
     2881 aaggaattct taggtacata aatcttagta aagcccagtt tagcagcttc gttgatgcgt
     2941 tgctcaatac gattcacgcg ccgaatctct cctgtcaagc ccagttctcc gacaaaacat
     3001 tcctgaggat tagttggctt gtctttgtag ctcgaagcaa tagcaactgc aacagccaag
     3061 tcaatcgcag gttcatccaa tttaacacca ccagcagatt tgagataggc atcctgattt
     3121 tgcaagagaa gccctgcccg tttttccaaa acagccataa tcaagctagc acggttaaaa
     3181 tcaagtcctg tcgtagtacg cttggcattt ccaaacatgg tcggtgttac caaagcctga
     3241 acctccgcca aaatcggacg cgtcccttcc atggttacaa cgatggagga accagtcgcc
     3301 ccatccaaac gctcttctag gaaaacttga ctcggattga gtacctcaac caagccgccc
     3361 gactgcatct caaaaatccc aatctcatta gtggaaccaa aacgattttt gaccgctctc
     3421 aaaatacgaa aggtgtggtg acgctcccct tcaaagtaaa gcaccgtatc caccatatgc
     3481 tccaacatac gaggcccagc caaggttcct tctttggtca catgacctac gataaagatg
     3541 gcaatgttat tggtcttggc caactgcatg agttcagcgg tcacttcacg cacctgagaa
     3601 acagacccct gcacccctga aatctcagga gacatgatgg tctggatgga atcaataatg
     3661 agaaagtctg gctggatacg ctccacttct gcacgaacac tctgcatatt ggtctctgca
     3721 tagagataaa actcactatc aatatcacct aagcgctctg cacgtagttt aatctgctgg
     3781 gcagactcct ccccactgac atagagaact gtccccactt gggacaactg ggttgagact
     3841 tgtaggagaa gagttgattt cccaatccca ggatccccac cgatgaggac gagacttcct
     3901 ggtaccactc cgcctccaag cacacggttg aattcctcca tctccgtctt agttcgattg
     3961 acattgatgg aagtcacctc agctagtttc atgggcttgg ttttctcacc tgtcaaggac
     4021 acacgcgcat tcttgacctc ggcaacctca acctcttcca caaaagaaga ccaagaccca
     4081 cagttggggc aacgtcccag atatttagga gaattatacc cacaattttg acatacaaat
     4141 gtcgcttttt tctttgcgat gacaaacctc tttctatatc tctaactcac actcaatcac
     4201 ttggcaaaaa tcaatcttct catttggcac aaactggcgc atgagcattc gatgagcaac
     4261 aactaccaca gtctgatgtt ctcgatactt agacatacat tctagaaacc gagacttcat
     4321 ttccgtagct gtctcatatt gaataggact attaggaagc atctccccct tgttttctaa
     4381 aaacagtctt ctagctgttt caaagttttc tattcctgtt ttatagacct gccattcatg
     4441 taataaaggc tctactctta aaggaagacc cgtagcacag accacatacg aagccgtttc
     4501 taaagctctt gtgactgcag aagatacgat tatttcagct gacgagagta aaggattttt
     4561 gctcaatttc tggacttgct gccgtcccat ctcagacaag ggtgccaaat ctatcccaaa
     4621 tcctatataa gaacgctcct ctaactcacg gtaatctggc tccccatgac gtacaaagat
     4681 aatcttcatt ctagtgccct gtcgatccaa atccaccagt tcgaacgcca ccagctgcat
     4741 ctccatctgc aattaagaaa gtagcaaaaa cagcctggac aatacgctcc ccaacttcaa
     4801 gaacaacctc ttggtctgtg atattcttca tctgcgcaaa aatatgccct tcatttccag
     4861 gatttccata ataatcccca tcaatgaccc caactgagtt aattaaaacc aagcccttct
     4921 tacgaggatt tgaagaacga tcatagaggt agagaacctc agtcggctgc atataagcct
     4981 taacccctgt cggaaccaag acaatctctc ctggcgcaac aactgtacgc acagcaacct
     5041 ttaagtcgta accagccgca tgcgctgtct cacgcttggg caataaattt tcatctgtaa
     5101 aactcgaaac caattcaaaa ccacgaattt tcataatttt ctcttttcta ttatcattta
     5161 ttctagatta ttctatctta tttattcgga aaaagcacga aaaaagagca cacaattcaa
     5221 atcgctcagg gctgctggat tcctcccctg acccgcttca cgcagaactg ttgctccact
     5281 atttattata tcacattcct attcatttta aaagcaaaat tattttttcc gtctatttct
     5341 aaaaaaatcc tgcataatag ctgcgcattc atcttccaaa attcctattt caacctccac
     5401 acgatggtta agacgctcat ctgtcaagat atcgtacaaa ctcccagcag cgccaaattt
     5461 ctggttttta gccccataga ccacatttgg aatgcgggca agcccaatcg ccccactaca
     5521 catgacacaa ggttcaatgg tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc
     5581 actcaagttc gcatcctcta tagccataat ttccgcatgc ataaccgctc gctgtaattc
     5641 ctcacgcgca ttatgcccac gaccaatgat ttccccatct ttgacaatca cacaaccaat
     5701 tggaatttca tcgtgttcaa gagcaatctc agcctctctc aaagcctccc tcataaagac
     5761 ttctttttct tcaagcgtat aatacatctc ttttctcttt tcctacttat cgattttatt
     5821 attatatcat gaatcccaac acaaaaaaag ccaccgaatg cggtgactct atagggagat
     5881 tattatgaaa aagaaaagtt taggatattt gttacaacaa gttaggaggt cttcttgtaa
     5941 ccgtctatag tatacccgac ctatcttaaa caaatcttaa aaatctctta ggaccaaaca
     6001 ctttctaaaa tatttgtttg ttcacgacca ggacctactg agaaagtaga aatacgaacg
     6061 ccaaccaatt cactcacacg acgaacatag ttacgcgcat tctcaggaag atcttccaaa
     6121 ttgcgaactc cggtaatatc ttctgaccaa cctggcaact cttcatagat aggcttgcaa
     6181 cgtttcaatt gctcaagact agctggatag tagtcaatac gttgaccgtc aagatcatag
     6241 gccacacaga ttttcacagt atccaaaccg ctcaaaacat caatagagtt caaagaaagg
     6301 ttagtaatac cagaaacacg acggctatga cgcatcacaa ctgagtcaaa ccaacctaca
     6361 cgacgtggac gaccagttgt tgtaccatac tcatgaccca cttcacggat acgttctccc
     6421 acttcatcaa acaactcagt tgggaaagga ccatctccta cacgactcgt ataagcttta
     6481 catacaccta caaccttgtc aatcttgctt ggaccgacac cagaaccaat tgtcacacca
     6541 ccagctacag ggtttgatga cgtaacaaat ggataagtac cttggtcgat atctagcata
     6601 acaccttgtg caccttcaaa aagcacacgt ttgccattat caagcgcatc attcaagata
     6661 acagatgtat ctatcacgta tttcttgatt tgttgaccat attcgtaata ttcttcaaaa
     6721 atatcatcga aaacaatcgc tttactgtca tacaattttt caaaaagacg attcttttca
     6781 gcaaggttac gttctaaacg ctcacggaaa atatctttat ctaaaagatc tgcaatacga
     6841 attccaacac gagcagcctt gtccatataa gctggaccaa ttcccttaat tgtcgtacca
     6901 atcttattgt cgcccttagc ttcttcttgc aagcgatcca actcgatatg ataaggcaaa
     6961 ataacatgcg cacgatcaga aatacgcaag ttatcagttg ttacaccttc ctcatgaaga
     7021 tagctcaact cttttacaag agatttagga tttacaacca taccattccc aatgacagat
     7081 attttttcag ggaagaaaat cccagatgga atcaagtgca acttaaattt cttaccgtca
     7141 atcacaatcg tgtgaccagc attatcacca ccttggtaac gtgcaatcac ttctgcattc
     7201 gctgaaagga agtctgtaat cttcccttta ccttcatcac cccattgggt acctacaaca
     7261 acaactgaag tcataatctt gtctgagccc tcaggctctt cctttctcac atacatggca
     7321 ggactctcac ctgcaattat atcttacaat ttattataat aaa
//
LOCUS       DAVMHG010000103         7343 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMHG010000103 DAVMHG010000000
VERSION     DAVMHG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.105"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            508..733
                     /locus_tag="VYR08_001629"
                     /pseudo
     CDS             508..733
                     /locus_tag="VYR08_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1477..2982)
                     /gene="pyk"
                     /locus_tag="VYR08_001630"
     CDS             complement(1477..2982)
                     /gene="pyk"
                     /locus_tag="VYR08_001630"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV3467130.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(3041..4048)
                     /gene="pfkA"
                     /locus_tag="VYR08_001631"
     CDS             complement(3041..4048)
                     /gene="pfkA"
                     /locus_tag="VYR08_001631"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV3467131.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4131..7232)
                     /locus_tag="VYR08_001632"
     CDS             complement(4131..7232)
                     /locus_tag="VYR08_001632"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV3467132.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLDITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVELLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVAPLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2244 a         1618 c         1278 g         2203 t
ORIGIN      
        1 aattcctaga aatgttttag cagctacagc gtactattcc aaactcaatc aactataaaa
       61 caatatagac tagtaatggt tggactcaat aagaattggg tactaagata ggtataagta
      121 aaaacaccat tagaaattat gaaaaaaggg ttaggtctac taagaaaaat actatatttg
      181 acttagttaa agtattcagt agcttgattg atgccctctt ttctccagtt caaaaggatt
      241 ctcctagcga tatccaatct atctacgacc aacgggcacc acccagacaa ggtaaagttc
      301 tgacctatgc cgagaggcaa ctgtatgatc agaaaaacga agtatcgtaa aaatcatcag
      361 actggacgac tacagacaga ctacttactc tcgtgttagg tagtttatct ctgcaggtag
      421 ccgctcgatg caggacaacg atttagatat ggagtttcca ttatcccaga cgactacgac
      481 ccaatcgctt atgtcgtcag caagtccatg gagccaaaga taagaaacag cgactaccta
      541 tttatcaaga ataccccaca gattgactat aataccatcg gcatcttcca agtagacggc
      601 gctaactatg tcaagaaact ccgccaaggc taccttaaaa aaactcaatc ccaaataccc
      661 cgacatccac ctagacgaaa gcaaccatat tcgtaccatc gggaaagttg tcaggctata
      721 tagggagaat taaaaaaggg ggagaatggc gatataaaac aaacaaattt taaaagattc
      781 gaaaaacttt gaaaaagttc attctacatt tcaaaaaaag aataagtgac ctaaaataga
      841 ttagattttg ttaaacaata gtgatatgta aatttagaga aaggcattta acatcacttt
      901 gcaaaaggca aatactaaac tagagaaacc gataaaccag ccccttagtt cccatatttt
      961 caggcacaca ctccttagta cactagctga gaagaatgct ataatggtta gagtggaaca
     1021 caaaaacgct aaaacgctaa aacagtaaat aacatctaca ctcacgtaag taaaagaatg
     1081 gagcaagcta ctttggaggt tttaaatacc atatcattaa atcgaaaata tatcagatcg
     1141 aatctagaca agtacattac gattgctaaa acatttgtag aaattcattt gactttctaa
     1201 tcaatttgtg catatcttat ttcatactca atgaaaatca aagagcaaac taggaagcta
     1261 gccgcaggtt gctcaaagca ctgctttgag gttgtagata gaactgacga agtcagtaac
     1321 atatatacgg taaggcgacg ctgacgtggt ttgaatttga ttttcgaaga gtatcaatcc
     1381 attatgaaag ttttgccccc ctttttgccc cctggacaaa aaaagagccc atcatacagg
     1441 ctagaaagct ggatatgata ggtttttata tttttcttaa cgtactgtgc ggatacgcat
     1501 tgtgtttgtg cgaacagctt ctcctactgg cacaccagca acgataacga tatcatcgcc
     1561 tgactcaacg agacctgctt ctaccgcttt acgttcagcg atttcgaaca tatcgtcagt
     1621 tgaagatgga gcatctgtca acattgggat aacaccccag ttcaacatca agccacgttc
     1681 tgtcaattcg tcaaatgtca atgctaagat gtcagcattt ggacggtatt tagaaatcaa
     1741 acgtgcagta tgacctgtct tagtaagagt tacaaccaat ttgatatcca ttgagctagt
     1801 agcatcttta acagcagaag ccattacttc tgtcttagag ttacgctcaa atgaatctga
     1861 atcaagacgt ccgtattcat taagaagagc ttgagcgttc ttgtcgattg tagccattgt
     1921 agttactgac tcgagtgggt atttaccgtt tgcagactcg cctgacagca ttgtagcgtc
     1981 agttccgtcg ataacagcgt tgaatacgtc tgatacttct gaacgagttg cacgtggttt
     2041 ttcagtcatt gtttcaagca tgtttgttgc agtgataaca actttacctg cagcattgac
     2101 tttcttgata atcatttttt gataaactgg aaccatttcg aacggtactt cgatacccat
     2161 atcaccacga gcaatcataa taccatcagc tgcttcgata atttcgtcta agttatcgat
     2221 accttgttgg ttttcgattt tagcgaacaa ttgaacatgt ccgtttccag tttcttcaca
     2281 gattgcacga acttcgttca catcttttgc agtacgtacg aatgaaattg cgatgaagtt
     2341 gataccttgt tcaagaccga aacggatatc gtcgttatcg cgttcagcaa gagctgggaa
     2401 aggaatttta gtgttaggga tgttcacacc tttttgttta gcgatgatac catcgttttc
     2461 aacttcaact tcaaattcac gagttgcatc atctttagca accacacgaa gaccaagttt
     2521 accatcgtca accaaaactt gacgaccaac ttcaacatca tcatagatat caagagcacc
     2581 agcaacgttc aacgcaatca cttcacgagt tgatttgatt ccttgtttag ttgcaacacg
     2641 aattttttca ccagttttgt atgaatattc tttagcttca ccttcgaaca attctgtacg
     2701 gatttcaggt ccttttgtat caagaaggaa accaactttt ttacctgcaa ttttttccgc
     2761 aagtttaaca gttgccatac gctcaccttg ttcttggtgg tcgccgtgtg agaagttgaa
     2821 tcggaatgtg ttagcaccag cttcaatcaa tttagcaatg tttttagctg aagcttcaac
     2881 atcaagtttt tcaccccagt atccgtcctc accgaatttt ttaccaccac ggatttctac
     2941 cgcaggaccc aaagttgcaa cgatttttac acgtttgttc atgatttttg tgactccttt
     3001 atatatatga cctttttggt cttattaact aaattgtaaa ttatgacaag ctcttattca
     3061 agctagatag ctcaatatcg gctttgtgtg ggttgttaac cacaatctta ccttctgcag
     3121 taaggctaaa caatgcccct tcttctgcag taccaagaat tggattttca accatttttt
     3181 cgttacgaat accaaccgca acaccaccga taccttcttt aagaagttta acagcatgtg
     3241 cacccatacg tgacgccaaa acacggtcac gcgcagttgg agaaccacca cgttgaatat
     3301 gtccaagttc tgttacacga aggtcgcttg tatctccagc ttctttaagt ttttgaccaa
     3361 attcagccgc tgacatcaca ccttcagcta agacgatgat attgtgtttt ttaccacatt
     3421 cataaccagc tttgatgctt gctacgatat cttccatctt gaagcctgct tcagggatga
     3481 tgatttcatc agcaccagtt gcaataccag cccaaagagc gatatcacca gcgttacgtc
     3541 ccataacttc gattacaaaa gtacgacggt gacttgatga tgtatcacga atcttatcga
     3601 tagcgtccat ggcagtagta accgctgtgt caaaaccaat tgtaaagtca gtaccaacga
     3661 tatcgttatc gattgtacct ggaagaccaa tagctgggaa gccatgttca gtcaaacgca
     3721 tagcgccgtg gtaagatccg tcaccaccga taacaactac accttcaatt ccgtgttttt
     3781 tcaattgctc aatcccttta agttgccctt caagttgagc gaactctgga taacgagctg
     3841 agtgaagaaa agtaccacca cgagaaatga tgtctcctac tgaagctgca tctaggggat
     3901 gaatttcacc ggcaaccata ccagcatatc cgtcatagat accaaacact tccattcctt
     3961 ctgaaattgc ttgacgaaca actgcacgga tagcagcgtt cataccaggt gcgtctccgc
     4021 cactagtcaa aacagcaata cgtttcatat tggtttatgc tcctttttct tttaacattc
     4081 ttcttgatta tatcacattt gattttaaaa ttcttctatt ttccgtattt ttagcgataa
     4141 atcgttttca taacgatttc attcaatttc tcctctaatt cattggattt agctacaaaa
     4201 tgatggggag aaacgatggt tttctgttcc tcttcatacc ggatgatgac tgggattggg
     4261 cctttaaatt gttctaaaat acgtgaaatt tcttgatccg attcatgatt tttcacctgt
     4321 atccaaaagc gttcagcaac tgcttctctt atttcttgtg caatcatttg cagacggcca
     4381 tcacgtgatt gtatttttcc ttttacatag tagaaggctc cctcttttat ttcctgtcca
     4441 acctgacgat ataagtctga aaagagagtg acatccaatt ttttcttact atcatctgcc
     4501 tgtaagaagg ccatattttc accctttttg gtacgaatca cttttatttt ctgaacttca
     4561 accaaaataa tagcatagct attttctgac aaatttccga ttggggtaat cgggtaaata
     4621 gccttacttg caatagcttg tagtggatgt ttgctgacac ctatccctaa aagctcttgt
     4681 tccatataaa atttttcttg ttccgtccaa tcttccgatt cctgccaact ataaatagca
     4741 tctccaaaca aacttcccaa ctctttcaca aattcaaata gattagctaa gttattaaat
     4801 actttttgac gatttttttc aaatgaatcg aaaagaccaa cttttaccaa aggttctagc
     4861 agaggaagtt tcagataatt ctcaggtaat ttagctataa aatcttcaat gttagaataa
     4921 ggtctatttt caataatcca gagagctaaa tcattactga ctcctttaat ggatttcaaa
     4981 cctagataga tggccttgtt ggcaatttta tcgtgatagg gaatcgtgtt gatggacaga
     5041 ggcgccactt caaaacctgc ttcaagtgca tctattaagt aatcactgtt ggcagaattc
     5101 aacataatct gataaaatat ggctggataa tgtgttttaa aataagccaa ctggaaggcc
     5161 aatgctgagt aggcataggc gtgtgacctg ttaaaaccat agcctgcaaa cttctccata
     5221 acatcaaaga cctgctctgc tttttccaca gtatgaccag cttctaatga accttgaata
     5281 aaggaagccc tcatctcatg catggcagag gcatcctttt tccccatagc ccgacgcaaa
     5341 atatcggctt tcccaagact aaatccggca aatcgctggg caacctgcat aacctgctcc
     5401 tgatagagca ttatgccgta ggttggagcc aaaatatcct ccagtactgg atccagaaca
     5461 gtcacttctt cctgcccatg ctttcttgcc acaaaattat tgatatagtc actagcaccc
     5521 ggtcgattta gagaagtagt cgcgacgaca tcttcaaaac agactggttg cacacgctta
     5581 agcagacgaa tggcacctgg ttgctcaaat tgaaagatac cttttgtatt accagaggca
     5641 aataaagcta acgtttcttt gtcttctaaa tcgatttctt caattttcag atgaatacct
     5701 tctgtttcag caagcaactc ttgcatcttc tggacaaagg tcaaatttcg tagtcccaga
     5761 aagtccatct tcaaaagtcc gctagcctca actccatgag catcatactg agtcagtgga
     5821 atttcatcac catactttag aggaatgtag ttggttaaat cttggtcact aattacaaca
     5881 ccagccgcat ggacagaggt ttgccttgga tagccctcta tcttgcaagc aatctcaaaa
     5941 gctttttggt attctaactt actattgatt tgctgacgaa actggagatt gccctcatag
     6001 gccgacttaa gattgtcacg aaaactgatt ttcttagtaa ttgcagataa ttcatactct
     6061 ggcacaccaa agcgtttcaa gacatctcga agagcttgct tggctccaaa ggttgaaaaa
     6121 gtaacgattt gtgccgcatg tttactacca tatttattac caacatatct gataaaatct
     6181 ggacgataaa tatctgggat atcaatatca atatcaggca tggtatagcg ttcacgatta
     6241 agaaagcgtt caaaaatcag atttttctct actgggtcaa tccccgtgat gtctaaggca
     6301 taagaaacca aactgcctac tgcagaaccc cttcccattc ccatataata gccattcgat
     6361 cgtccaaaac gcaacaaatc ccaaacaacc aagaaataat catcaaagcc catatcatga
     6421 ataacagaca attcttggtc tagtctatct tgatattctt tactagtcaa ccccttctga
     6481 acaagcccca gttcagcacg ctctctcaac tcctctactg ctggtctagc tggattaaaa
     6541 cgaggcagtt tcagactagt atccaagtcg taagaaatgc ctgaaataag cttttctaaa
     6601 ttgtccaaag cttgcggaaa acactcttgg aatagtttct ctaaagaact tgctgatata
     6661 aagacatctt gtctcgaacg caagggaact tctctgagcg gtagattttc tttaatcgct
     6721 gttaaaactt gaagaacttc tctatccctg ctttcaaaag cgttgacccg ataaagaggt
     6781 aagataggat gatgaaattc gcttgctagt gtttctggat aaacccctat atagtaatca
     6841 cagcctagtt ctaacaactc aactctatca aaataaggca caatgaccgc gatatcctcc
     6901 aggtactggg acaggactga ccaagttttc tccccctgca tcttggctgt cgaaagcttc
     6961 atcaactgct gatagcccac actagataga gctaaaaagc gcaaattcac ttcctgatca
     7021 tctacaaaca ctgtcatttc aagccctagc aaaggatgaa tgccgtattt ttttgtaata
     7081 tctagaaagt cgaaagcgcc ataaagattg tcaatatcca tcatagccaa atgagtgtag
     7141 ccgtattctt tagctgctct cacatacttt tcgatcgaaa tgacgctttc cataaaacta
     7201 tagactgttt ttgtatctag ttgtgcgatc aatttacact tctcctctat ccttctcact
     7261 atattatacc attttcacct ataaatggct tctcttgaga aaaatttcat cagcttacta
     7321 atttattttc tattcatttg tat
//
LOCUS       DAVMHG010000104         7249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMHG010000104 DAVMHG010000000
VERSION     DAVMHG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.106"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            97..1164
                     /locus_tag="VYR08_001633"
     CDS             97..1164
                     /locus_tag="VYR08_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467133.1"
                     /translation="MTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVLLLF
                     LFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIRVGI
                     NLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFPLLR
                     RTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFPMSV
                     YEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIVVLL
                     ALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGLYLY
                     LKHEDIKTYLAYFKASKKTSN"
     gene            1229..2317
                     /gene="wecB"
                     /locus_tag="VYR08_001634"
     CDS             1229..2317
                     /gene="wecB"
                     /locus_tag="VYR08_001634"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV3467134.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVTAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            2480..3349
                     /gene="rfbA"
                     /locus_tag="VYR08_001635"
     CDS             2480..3349
                     /gene="rfbA"
                     /locus_tag="VYR08_001635"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV3467135.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            3350..3943
                     /locus_tag="VYR08_001636"
     CDS             3350..3943
                     /locus_tag="VYR08_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV3467136.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            3955..5004
                     /gene="rfbB"
                     /locus_tag="VYR08_001637"
     CDS             3955..5004
                     /gene="rfbB"
                     /locus_tag="VYR08_001637"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV3467137.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            5070..5921
                     /gene="rfbD"
                     /locus_tag="VYR08_001638"
     CDS             5070..5921
                     /gene="rfbD"
                     /locus_tag="VYR08_001638"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV3467138.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            5984..6318
                     /locus_tag="VYR08_001639"
                     /pseudo
     CDS             5984..6318
                     /locus_tag="VYR08_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            6585..7170
                     /locus_tag="VYR08_001640"
                     /pseudo
     CDS             6585..7170
                     /locus_tag="VYR08_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         2283 a         1254 c         1554 g         2158 t
ORIGIN      
        1 attctatttt ctagcttctt aatgattatc tccttgctat tgtttggttt aaatgtatta
       61 gctgtaacag attcgaatgc acgatatatt tataatatga ctattataag tatgataatc
      121 acaaatttaa gaacgctcta cgtgtatgtt ttgcagatga caaaccgctt gaaggatagt
      181 tccatcattc tactcagtga tcgcgtttta tatgtgctcc ttttattcct gtttattgta
      241 tttggttggc atgaatacaa ggttatgatt ttggcagata ttctaggtcg atcattttct
      301 ctcatacttt ccttctggat ttgtaaagat attgtgtttc agcctttgtc aaaattcatc
      361 tttaatataa aagagtcttt tgataatatc cgagttggta tcaatttaat gttatctaac
      421 atcgcaagta gtttgattat cggtattgtt cgtctaggaa tccaatggaa ctggaatatc
      481 gaaacattcg ggaaagtatc tctgaccttg agtgtttcca atctattgat gactttcatt
      541 aatgccattg gactagtcgt ctttccgcta ttaagacgaa caaaagcaga aaacttacct
      601 aagatttatt ctaatttaag aaacgttttg atgcttatca tgttcgcgat tttgctcatt
      661 tactatcctt taaaaattgt attagacctc tggttgccag cctatcaaga tgccttgatt
      721 ttcatgaccc ttattttccc tatgtcagtc tatgaaggga aaatggcatt ggtcattaat
      781 acttacttaa aggcattaag aatggaaaga gatatcctta gagtcaatgc cttagtaatg
      841 ttgatcagta tgggagtgac cctggttaca acatacctgt taaatagttt ggagctgact
      901 gttgtatcga tagttgtttt gctagctttg agaagtatca tagccgaatt aattctgtct
      961 aaaaaactgg atgtttcggt taagaaggat attgtattag aatttctttt gacgcttgtc
     1021 tttatttctt caagttggta tttgccgatt gggctggcag taattgtcta cacaatagcc
     1081 tacggtttat atctctattt gaaacacgaa gatatcaaaa cctatttagc ttactttaaa
     1141 gctagtaaaa aaacatcaaa ttaaaaaatt atataatgat taagtggtag attctatttc
     1201 taccgtttta gatattcggg aggtaatgat gaaaaaaatt atgttagtat tcggtacacg
     1261 tccggaagcc atcaaaatgt gtccattagt caatgagttg aaaaaacacg aagatatgga
     1321 aacaattgtg tgtgttactg gacaacacaa agagatggtt agtcctgttt tagatttatt
     1381 tggtgttgta ccagattatg atttagaaat tatgaaggct aaccaaacct tgttctctat
     1441 cacaactagt atcttggaaa agataaaacc agttttagag aaggaacaac cagatattgt
     1501 cctagttcac ggtgacacta cgacaactta tgcagcagcc ttggcagcat tctatttggg
     1561 aattaaagta ggacatgttg aagctggttt gcgaacgtac aatttacaaa gtccatttcc
     1621 tgaagaattt aacaggcaat cgacatcaat cattgcaact taccattttg ctccaactga
     1681 gttggctaaa gaaaatctct taaaagaagg tagagagaat gtttatgtga ctggaaatac
     1741 tgtcattgat gctcttacaa ctactgttca agaggattat acacacactc atttagattt
     1801 aaacgctaac aatcgtctca tcttattgac tgctcataga cgcgaaaatc tcggcgaacc
     1861 gatgagacat atgtttagag cagttaaacg agtattgaat gaatacgaag atgttaaagt
     1921 catttatcca attcataaga atcctttggt acgtgaaaca gctgcagaga tctttggaga
     1981 tacagaacgg attcaaatta ttgaaccttt ggatgttctt gatttccaca acttcatgaa
     2041 ccaaagttat atgattttaa cagattctgg cggagttcag gaagaagcac cttctttagg
     2101 aaaacctgta ttggttatgc gcgatactac tgaaagacct gaaggagtaa cggcaggaac
     2161 cttgaaatta gtaggaactg acgaagaaac aatttatcaa aacttcaaac tacttctaga
     2221 tgattctgga gaatacaaaa aaatgagtca ggctagcaat ccatacggta atggtgatgc
     2281 tagtcaacag attgttcaga ttttgcgtgg gatttaagaa gattctctaa aagagtctaa
     2341 gaggaatcca ctccacaaac ataaaactct tatgctatca tttatgattg agatagactc
     2401 ataacttaat atttttatcc taagaaaatt cgttcatatt caatatgaat gaaaaacaac
     2461 gaagaaaggt tatttcatta tgaaaggtat tattcttgca ggcggctcag gtacccgcct
     2521 gtacccactt actcgggctg cgtcaaaaca gctgatgccg gtttatgata aacctatgat
     2581 ttattatccg ttgtcgacat taatgttggc tggaattaaa gatattttga ttatctcaac
     2641 tcctcaagat ttgccccgtt ttaaggactt gctcttggat ggttccgaat ttgggatcaa
     2701 gctttcctat gcggaacaac ctagtcccga tggacttgct caggcttttc ttatcggtga
     2761 agaatttatc ggtgacgata gcgttgcctt gattttaggc gacaatatct accatggtcc
     2821 tggtctgagc acaatgcttc aaaaagcagc caagaaagag aaaggtgcga ctgtttttgg
     2881 ctaccaagtg aaggatccag agcgttttgg tgtggttgag tttgatacag acatgaatgc
     2941 tatttctata gaagaaaaac cggagtatcc tcgctccaac tatgcagtga caggactgta
     3001 tttctatgat aatgatgttg tggagattgc taaacagatc aaacctagtg ctcgtggcga
     3061 gttagaaatt acagacgtta acaaggctta cctaaatcgt ggtgaccttt ctgttgagct
     3121 gatggggcgt ggttttgcct ggttggatac gggaacccat gaaagcttgc tagaagcttc
     3181 tcagtatatt gaaacggttc agcgtatgca gaacgttcaa gtggcaaatc tagaagaaat
     3241 ttcctatcgt atgggctaca ttagtcgtga agacgtgctg gaattggctc agcctcttaa
     3301 gaaaaatgaa tacggacaat atttgctccg tttgattgga gaagcataga tgtcagataa
     3361 tttttttgga aagacacttg tggtacgcaa gattgatgct ataccaggac tgctagagtt
     3421 tgatattccc gttcatggag acaatcgtgg ttggtttaag gaaaatttcc agaaggaaaa
     3481 gatgctaccg cttggttttc ctgaaagctt ctttgctgca gggaaactgc aaaataacgt
     3541 cagcttttct cgcaaaaatg ttcttcgagg actccatgct gaaccttggg acaagtatat
     3601 ctctgttgca gacgatggga aggttttagg atcttgggta gatttgcgtg aaggcgagac
     3661 ctttggaaat gtttaccaga cagagattga tgctagcaag ggaatttttg tccctcgtgg
     3721 cgtagccaac ggttttcaag tcctctcaga tacggtttcc tatagctatc tggtcaatga
     3781 ctactgggct cttgaactca aacccaaata tgcctttgtt aactatgctg acccagcatt
     3841 gggaattgag tgggaaaacc tagcggaagc tgaggtttca gaggcagaca aacaccatcc
     3901 tctattaagg gatgtcaaac cacttacgaa agacgagttg taaaaggaaa cattatgact
     3961 gaatacaaaa aaattatcgt gacaggtgga gctggtttta tcggttctaa ctttgttcac
     4021 tatgtttaca ataactttac agatgtccat gtgacagtgc tggacaagct gacttatgca
     4081 ggtaatcgtg ccaatattga agaaatttta ggcgaccgtg ttgagttagt cgttggagat
     4141 attgctgatg cagccttggt agataagtta gcagctgaag cggatgccat tgtacactat
     4201 gcggcagaaa gccacaatga caattcgctt aatgacccct cgccatttat ccataccaac
     4261 ttcatcggaa cttatacact cttggaagcg gctcgtaaat acgaccttcg tttccaccat
     4321 gtgtcgactg acgaagtcta tggggatctg ccgctgcgtg aagatttacc aggtcatggg
     4381 gaaggaccag gtgagaaatt tacggctgaa accaagtaca atccaagctc gccttactca
     4441 tcgactaagg cggcttcaga cttgattgtc aaagcttggg tgcgctcatt tggtgttaaa
     4501 gcaacgattt ctaactgttc aaacaactat ggtccttacc agcacatcga gaagttcatt
     4561 ccgcgccaga ttactaatat cttgagcggt atcaagccaa aactctacgg agaaggtaag
     4621 aatgtgcgtg actggattca tactaatgac cattcatcag gtgtttggac gattctgacc
     4681 aagggtcaaa tcggtgaaac ctacctgatt ggtgcggatg gtgagaagaa caataaggaa
     4741 gtgttggaac tcatcctcaa agaaatggga cagccagctg atgcttatga ccacgtaaca
     4801 gatcgagctg gtcacgacct ccgctatgcc atcgatgcaa gcaagctccg tgatgaacta
     4861 ggatggaagc cagagtttac caactttgaa gcaggtctca aagcgactat caagtggtac
     4921 acagataacc aagactggtg gaaagcagaa aaagaagctg ttgaagccaa ttatgctaag
     4981 actcaggaga ttattacagt ataaaaagca ggaaatcgct gctttttatt gctatattgg
     5041 gaagagttac atattagaaa ggtctagaga tgattttaat tacaggggca aatggccaat
     5101 taggaacgga acttcgctat ttattggatg aacgtaatga agaatacgtg gcagtagatg
     5161 tggctgagat ggacattacc aatgaagaaa tggttgagaa agtttttgaa gaggtgaaac
     5221 caactttagt ctaccactgt gcagcctata ctgctgttga tgcagcagag gatgaaggaa
     5281 aagagttgga cttcgccatc aatgtgacgg ggacagaaaa tgtcgccaaa gcatctgaaa
     5341 agcatggtgc aactctagtt tatatttcta cggactatgt ctttgacggt aagaaaccag
     5401 ttggacaaga gtgggaagtt gatgaccgac cagatccaca gacagaatat ggacgcacta
     5461 agcgtatggg ggaagagtta gttgagaagc atgtttctaa tttctatatt atccgtactg
     5521 cctgggtatt tggaaattat ggcaaaaact tcgtttttac catgcaaaat cttgcgaaaa
     5581 ctcataagac tttaacagtt gtaaatgacc agtacggtcg tccgacttgg actcgtacct
     5641 tggctgagtt catgacctac ctagctgaaa atcgtaagga atttggttat tatcatttgt
     5701 caaatgatgc gacagaagac acaacatggt atgattttgc agttgaaatt ttgaaagata
     5761 cagatgtcga agtcaagcca gtagattcca gtcaatttcc agccaaagct aaacgtccgc
     5821 taaactcaac gatgagcctg gccaaagcca aagctactgg atttgttatt ccaacttggc
     5881 aagatgcatt gcaagaattt tacaaacaag aagtgagata agtagtagaa tgattttcta
     5941 gtctaataaa agaggcagat aatgaactca aaaggagctt aaggtgtacg attatcttgt
     6001 tgttggtgct ggtctctttg gtgcagtctt tgcccatgaa gtagccttaa aaggaaaaaa
     6061 gtaaaagtca ttgaaaaacg gaatcatatc gtgggtaata tctatactcg tgaagaggaa
     6121 ggaattcaag ttcatcaata tggtgctcat atcttccata cttctgataa ggagagctgg
     6181 gattatgtaa atcagtttgc agggtttaac cgttacacca attctcctgt tgcaaactat
     6241 aagggtgaga tttataacct tccttttaat atgaatactt tcaataaact ctggggagtt
     6301 gtaacgccag cagaagcaca agctaagatt gaggaacaac gtgctatttt aaatggtaaa
     6361 actcctgaaa atttggaaga acaggcgatt tctcttgtag gtacagacat ctacgaaaaa
     6421 ttaatcaaag actatacaga gaaacagtgg ggcaaaccaa ctactgaact tccatccttt
     6481 attattcgcc gtttaccagt acacctgacc tatgataaca actattttaa cgatacctat
     6541 caagggattc caattggtgg atacactcaa atagttgaaa aatgttggat tatgaaaata
     6601 ttgatgtaga aacaaatgtt gatttctttg tgaacaaaga gcaatatctg aaagattttc
     6661 ctaagattgt ctttactggt atgattgatg aattctttga ctataagttg ggcgaactag
     6721 agtaccgtag tcttcgtttt gaaaatgaga ccttggatat ggaaaattac caaggaaatg
     6781 cagttgtgaa ctatacggat gcagaaaccc catatactcg cattattgaa cacaaacatt
     6841 ttgagtttgg gagtcaagca aagactatca ttactaatag aacattctaa aacatgggaa
     6901 aaaggtgatg agccttatta tccagttaat aatgatcgta ataatcattt gtataaatca
     6961 tataaaaaac ttgctgatga gcaagggaat gttatctttg gtggccgctt aggacactat
     7021 cgttattacg atatgcacca agtaattgga gcagctttgc agtgcgtgag aaatgagtta
     7081 gattcatact caatgaaaat caaagagcaa actaggaagc tagccacagg ttgctcaaaa
     7141 tactgttttg aggttgcaga tggaagctga cgcggtttga agagattttc gaagagtata
     7201 aacaagtaaa actgactacc agttattatt tagaaatagt attaaaaat
//
LOCUS       DAVMHG010000105         7205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMHG010000105 DAVMHG010000000
VERSION     DAVMHG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.107"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            9..2321
                     /gene="rnr"
                     /locus_tag="VYR08_001641"
     CDS             9..2321
                     /gene="rnr"
                     /locus_tag="VYR08_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV3467139.1"
                     /translation="MTVNDLAQALGKDSSKDFRELIKTLSLMERKHQIRFEEDGSLTL
                     EIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAIDGDTVEVVIKKVAD
                     RNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQKISQPIYVKKPALK
                     LEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVLESMDIVSEFPEAVV
                     KEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIKALKNGNLELGVHIA
                     DVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSLNPQVDRLTQSAIME
                     IDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHKIVPSIELMAKLHET
                     LENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIESFMLMANETVAEHF
                     SKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQEALQDIMRAVEGEPY
                     ADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPDLLVHRMIRDYGRSK
                     EIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYVGEEYDAVVSSIVKF
                     GLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVGQQIRIRVERADKMT
                     GEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKSGRSNDKRKHSQKDK
                     KKKGKKSFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2284..2751
                     /gene="smpB"
                     /locus_tag="VYR08_001642"
     CDS             2284..2751
                     /gene="smpB"
                     /locus_tag="VYR08_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV3467140.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            2767..3627
                     /gene="tehB"
                     /locus_tag="VYR08_001643"
     CDS             2767..3627
                     /gene="tehB"
                     /locus_tag="VYR08_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV3467141.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            3700..4653
                     /gene="coiA"
                     /locus_tag="VYR08_001644"
     CDS             3700..4653
                     /gene="coiA"
                     /locus_tag="VYR08_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV3467142.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            4672..6474
                     /gene="pepF"
                     /locus_tag="VYR08_001645"
     CDS             4672..6474
                     /gene="pepF"
                     /locus_tag="VYR08_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV3467143.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6476..7189
                     /locus_tag="VYR08_001646"
     CDS             6476..7189
                     /locus_tag="VYR08_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV3467144.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
BASE COUNT         2235 a         1362 c         1638 g         1970 t
ORIGIN      
        1 agggaaaggt gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt
       61 ttcgtgagtt gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag
      121 aagatggtag tctgacatta gaaattaaga aaaaacatga gattaccctc aaggggattt
      181 ttcatgccca taaaaatggc tttggctttg ttagtctgga aggcgaggag gacgaccttt
      241 ttgtagggaa aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta
      301 agaaagtcgc tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag
      361 aacacagttt gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg
      421 ctggctatat tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag
      481 ccctaaagtt agaaggaaca gaagttctca aagtctttat cgataaatac ccaagcaaga
      541 aacatgattt ctttgtcgcg agtgttctcg atgtagtggg gcactcaacg gatgtcggaa
      601 ttgatgttct tgaggtcttg gaatcaatgg acattgtatc tgagtttcca gaagctgttg
      661 tcaaggaagc agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg
      721 atctaagaga tgaaattacc tttaccattg acggtgcgga tgccaaggac ttggacgatg
      781 cagtgcatat caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg
      841 tttcctatta tgtgaccgag ggttctgccc ttgacaagga agcccttaac cgtgcgactt
      901 ctgtttacgt gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct
      961 gctctctcaa tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac
     1021 atggtcgtgt ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga
     1081 cctatagcga tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaataccata
     1141 aaattgtacc aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg
     1201 tgaaacgtgg agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag
     1261 gtaagcctgt tgatatcgtt cttcggcagc gtggcattgc cgagcggatg attgagtctt
     1321 ttatgttgat ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta
     1381 tctaccgaat tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt
     1441 cgagttttgg cttgcgtatt tatgggactg ccagtgagat tagtcaggag gcacttcaag
     1501 acatcatgcg tgctgttgag ggagaacctt atgcagatgt attgtccatg atgcttcttc
     1561 gctctatgca gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg
     1621 actattatac tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta
     1681 tgattcggga ttacggccgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag
     1741 agattgcgac ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg
     1801 aagccatgaa aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg
     1861 tatcaagtat tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga
     1921 ttcacatcac taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg
     1981 gagaaaaatc aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag
     2041 cggataaaat gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg
     2101 aaaaaggctt gaaacagtct agtcgtagtg gcagagggcg tggttcaaat cgtcgttcgg
     2161 ataagaagga agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa
     2221 aagacaagaa gaaaaaagga aagaaatctt tttacaagga agtagctaag aaaggagcca
     2281 agcatggcaa agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat
     2341 acaatcgtag atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta
     2401 cgagctgctc gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg
     2461 ctgagcaatg ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa
     2521 cgtcgtcgta aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa
     2581 gggacaggaa tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt
     2641 cttttaggac ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt
     2701 gagcaaaatc gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa
     2761 ttgaaaatgg aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg
     2821 cctgaagctg ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc
     2881 ttgaagggag ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac
     2941 ctctttgaag caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa
     3001 gctgccacag atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt
     3061 gctaaaaaat acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg
     3121 aaacaaggga aagctttgga tttaggttgt ggtcaggggc gtaattctct ttttctagcc
     3181 cagcaagatt ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa
     3241 agcattgtgg agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct
     3301 agcattgaac aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg
     3361 gaccgcattc cagctattat tcaaaatatg caggagaaaa ccagtgttgg tggttacaac
     3421 cttatcgttt gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc
     3481 tttaaagaag gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa
     3541 aatccaggcc atttgcatcg tcgcgatgag aatggcaatc gtattcaact acgctttgcg
     3601 accttactag ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt
     3661 ttttcttttt tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct
     3721 aggggagaat tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca
     3781 gcttgtggag gccagatcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat
     3841 aaatccttaa aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat
     3901 aaggaatccc tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt
     3961 tcagaactta aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag
     4021 tgtagtccct tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt
     4081 taccaagtac tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta
     4141 cagcaaggtt ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag
     4201 gaaaaacaag ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat
     4261 tatcaaatca aggaattttc ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc
     4321 tataagagac aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat
     4381 atccggcaac aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat
     4441 caaaagggag aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca
     4501 atagtgggca aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat
     4561 acctattacc aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat
     4621 cagcaatatt tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta
     4681 caaagaaatg aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac
     4741 caggcttggg aagaagcctt aaaagattta acagaacaat tggagacagt agcccagtat
     4801 gaaggccatc tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa
     4861 atggaacgcc agatggagaa gctttacgtt tatgctcata tgaagaatga ccaggataca
     4921 cgtgaagcta agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac
     4981 caagcctttt cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac
     5041 tttttagaag ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa
     5101 ggcaaggatc acgttctttc acaacgtgaa gaagaattat tggctggagc tggagaaatc
     5161 tttggttcag caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat
     5221 gtactagacg atgatggtaa agaagttcag ctatctcatg ggacttacac acgtttgatg
     5281 gagtctaaaa aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc gacttacgaa
     5341 caattccaac acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt
     5401 gctaaagttc gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca
     5461 gaaagtgttt atgacaattt ggtagcagca gttcgcaagc atttgccact tttacatcgc
     5521 tatcttgagc ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac
     5581 acaccgcttt catctgttga atacagtttt acctaccaag aagccttgaa aaaagcagaa
     5641 gatgctttgg cagtcttggg tgaggattac ttgagccgtg tcaaacgtgc cttcagcgag
     5701 cgttggattg atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct
     5761 tacgatacca atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact
     5821 cttgttcatg aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat
     5881 gtttacggag attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc
     5941 ttgacggaga aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat
     6001 aacttcctag atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa
     6061 cacgccattc accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa
     6121 ctctacgcag acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc
     6181 caatacgagt gggctcgcat tccacacttc tactataact actatgtata tcaatattca
     6241 actggctttg cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac
     6301 cgtgaccgct atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg
     6361 agaaaagctg gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt
     6421 gaacgccgtt taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt
     6481 tgaatcgtat agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat
     6541 tgtagacttg atgcgtcagc gtcaaaagca ggtcacaggt ttcttgaaag aattggaaga
     6601 ctttgcccgc aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt
     6661 tcttatggaa accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc
     6721 agctctcttg atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc
     6781 agaaatgatt ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac
     6841 tctcctagag ggagatgcgg tggatgtctt atctacactg acagagtctt atgatttcgt
     6901 ctttatggat tctgccaagt ctaaatacat cgtctttctg ccagaaatcc tcaaacattt
     6961 ggaagttggt ggtgtggttg tcttggatga tatttttcaa ggtggtgatg ttgccaagga
     7021 tattatggaa gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa aattatttga
     7081 tgcaacctta gacaatccag aactcaccgc aacattagtg cctttaggag atggtattct
     7141 catgcttcgt aaaaatgtag cagatgttca actgtctgaa agcgaatgat tttcagaaaa
     7201 attta
//
LOCUS       DAVMHG010000106         7004 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMHG010000106 DAVMHG010000000
VERSION     DAVMHG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.108"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..949)
                     /gene="lytB"
                     /locus_tag="VYR08_001647"
     CDS             complement(<1..949)
                     /gene="lytB"
                     /locus_tag="VYR08_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV3467145.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGVFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEW"
     gene            complement(991..1929)
                     /locus_tag="VYR08_001648"
     CDS             complement(991..1929)
                     /locus_tag="VYR08_001648"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV3467146.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1940..2740)
                     /locus_tag="VYR08_001649"
     CDS             complement(1940..2740)
                     /locus_tag="VYR08_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV3467147.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2990..3370)
                     /locus_tag="VYR08_001650"
     CDS             complement(2990..3370)
                     /locus_tag="VYR08_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3467148.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3428..3787)
                     /gene="rplT"
                     /locus_tag="VYR08_001651"
     CDS             complement(3428..3787)
                     /gene="rplT"
                     /locus_tag="VYR08_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV3467149.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3839..4039)
                     /gene="rpmI"
                     /locus_tag="VYR08_001652"
     CDS             complement(3839..4039)
                     /gene="rpmI"
                     /locus_tag="VYR08_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV3467150.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(4072..4602)
                     /gene="infC"
                     /locus_tag="VYR08_001653"
     CDS             complement(4072..4602)
                     /gene="infC"
                     /locus_tag="VYR08_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV3467151.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4618..4749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4909..6090)
                     /locus_tag="VYR08_001654"
     CDS             complement(4909..6090)
                     /locus_tag="VYR08_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467152.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     gene            complement(6094..6681)
                     /locus_tag="VYR08_001655"
     CDS             complement(6094..6681)
                     /locus_tag="VYR08_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467153.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6714..6902)
                     /locus_tag="VYR08_001656"
     CDS             complement(6714..6902)
                     /locus_tag="VYR08_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467154.1"
                     /translation="MTATKSYKYDWNTVLEYSINYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYSGGY"
BASE COUNT         2063 a         1564 c         1227 g         2150 t
ORIGIN      
        1 cccattcttt ttcagccatt ttgccatcag acttgaggta aaaccaagat tccttatccc
       61 aaatccattc attggctgtc atgtaaccac cggatttgaa gtagtaccaa gcttgactat
      121 gagaatcgta gacccattct ttttcagcta ttttcccatc aaacttgagg taaaaccaag
      181 attccttatc ccaaatccat tcattggctg ccatgtagcc accggatttt agataataat
      241 agtgaccatt ctcgaaaatc cattctttat cagcatagtt tccattttct ttgatgtaaa
      301 accaagattg gtattgtttg tcaaaaagcc aaccttgctg tactttggca ccactagcat
      361 tcacataagc ttgattaatc cactgacttg tcagtagata accaccggat ttgaaataat
      421 agtccttccc tttaatttgg agccattctt tctctgcgtg ctgtccatct gctttgatat
      481 aaaaccaagc atcgtattga gaatcataga cccagtcttc tattactttg gcacctgttg
      541 caccaacata ggaagttcct acccaagcat ttcttttcat ctttccatct tggtcaagat
      601 aataaaagac tcccttgtct tctacccatt ctgatttggc catatagcca ccagatttga
      661 ggtaaaaata gtcgtcacct tgctttagcc attcattatc agcatagtta gcatctgctt
      721 ttatatagaa ccaagattga taatgagtat caaaaaccca ctcattcgct gcttgactgc
      781 catcttcttt cagatactgt tttccttgcc aagtaccatc actagccatt gcaccctctg
      841 gactagctaa gaaaaatagc agagctaaaa aaataaatct tactttcttc attaaccttc
      901 ttcctctgtt cttatttatt ttattgtacc aaaattctcc taaattcata ttacaaacta
      961 ataaaaaatc aagaacagat tgattgcaat ttacctcaga gactctttta cttcctgacg
     1021 gagttcttcc agactgctaa taccgtattt atccatgact tttggtaaat tttcgatgat
     1081 gtcagggcag gcataaggat tggtaaagtt agctgttcca actccgatag cagatgcccc
     1141 agccagatac atttctaggg cagcttcagc cgaatccact ccccccattc caatgatagg
     1201 caggtctgtt gtttgggcta cttggcggat gagtttgagg gctactggaa agactgctgg
     1261 acctgacatt ccacctgttc cattggccaa gattggtttt ctggttttga ggtcaaagcg
     1321 cattccaacc agagtattga tcatagtcaa gccacttgct cccgcatctt ctgcagcttt
     1381 tgcgacagta acgatatcgg tcacactcgg ggttaatttg acataaactg gcacttctga
     1441 ggcttccaca gctgctttca ccacatcata agccaaatct ggatcttgac caatcaaaag
     1501 tccatgatta cagtggtcaa cattgggaca agaaatattg agctcgatag cttttacatt
     1561 agttaccttg gaaatcccat gagaaacagc tgcatactct tgttttgaaa aaccagccac
     1621 attggcaata ataggaagat ttggatattc tctttccagc caaggtagct tttcagccaa
     1681 aacaacctct aaaccaggat tttgcaagcc aattgcattg agcataccag caggcgtctc
     1741 tgccactctt ggagttggat ttccaaaacg tggttcaagg gttgtcgcct tgatcataat
     1801 agaacctaaa aggtctaaat catagtactt ggcatactct tgtccaaagc caaaacagcc
     1861 tgatgctgga ataatcggat ttttcaaatc caaaccaggt agagaaactt gtaatcgatt
     1921 tgtagtcata attttctcct tataatacaa ctgttcctgt gcggaaaaca ggaccatctt
     1981 cacagacgcg ttggctgacc gtctcgcttt ctggtacttt tagaacacag gcatagcaag
     2041 ctcccatccc acaagccata cgagattcca gagataaata ggctcttggg tgatcatcaa
     2101 aggtttgatt gatatacttc atcattcctg gagccccaca cgagtaaaca gcatcaaact
     2161 gactgtctaa atcattgata acaacggaaa catttccctt gatgccataa gaaccatcat
     2221 ctgtcgttac aaagacctga ccatactgag ccaattccgt tttcaaaata acagcatcct
     2281 tattagcaaa accgaggact gtcactactt tcactccacg ttcatgcaat tccttggcca
     2341 cctcaagcaa gggtggaaca ccaatcccac caccaacaag gagaacctga ttctgctcat
     2401 caaggtcaga caagtcaaaa ccatttccct gaggccccat cacatcaaga gtgtctccct
     2461 gacttaaggt tgaaaaaatt gcagtcccag ctccctcaat ccgataaatg aggtgacact
     2521 gcttgtttgc cttgtcaata gacgaaattg aaataggacg acgtaagaga tgggcatcgt
     2581 ctggtacacg cagatgaaga aattggcctg ctcgcatggc ttcaaccatt tctccttcta
     2641 ggactaattc aaagattgct ggcgcgattt cctcttgtgc aaccaccttc atggtttcca
     2701 accgaatgac acccaaacgc ttcttacatg tgagattcat aacttttcct ccttaaattt
     2761 taaggggacc agataaagaa aagaccttgc tagtgcaagg cctcagttaa aatcgtacaa
     2821 cacaagagag cacactcaaa aggtctcctc tagaaaattg cactattctt ttatctgaca
     2881 ccttgtgagt ctctctggac tccccttaaa ggttgaattt ttattagtat actctttcag
     2941 ctaaaaaaag tcaagtagaa aacgaatatt ctacttaact tgcacgaaat tatttttcac
     3001 gaatgacttc gaccttatat ccatcagggt ctttaacaaa gtaatagttt ggtgtagttc
     3061 ctggtagacc atttggctca gtcacttcat agccttttgc actgtgttct tgatgaagtg
     3121 cctcaagatc aggtgtactg agggcgatat gggcaaaccc atcaccaacc acatacggac
     3181 cgtgatcgta gttataagtc aactccaact cataatcatc accctcaaga cctagataga
     3241 caatcgtgaa ggcatggtct ggaaaatctc tgcgacgcaa ttctttaaaa ccaaaagcat
     3301 cttgataaaa tgcgattgat ttttcaagat tttctactcg taagcaagtg tgtagcattt
     3361 ttgaagccat atctttctcc tttattttta aaaagactgg acaatcctgt tccagtctca
     3421 tcagttgtta tttaccaagt tttgctttag ctgcatctgc aagagctgtg aaagctactg
     3481 catcgttaac agccaagtca gcaagcattt tacggttaac ttcgatctca gccaatttca
     3541 aaccatgcat caattgtgag tatgaaagtc cgttcatacg agctgccgca ttgatacgag
     3601 taatccacaa tttgcggaag tcacgttttt tctgacgacg gtcacggtat gcatagtagt
     3661 aagagttcat tacttgttct tttgcagtac ggaacaagat gtgtttagct ccatagtaac
     3721 cttttgctaa tttaagaata cgtttacgac gtttgcgtga tacaacgcca cctttaacac
     3781 gtgccatatt ttatttcctc caaatatttc ctagaattgt ttacttacag tcaagctatt
     3841 atttcaagcg agtaagcatt gctttgatac gtttgtaatc tcctgaatgc accatagatg
     3901 ctttacgaag atgacgacgt tgtttcttgg tttttccgtg gaaacggtga gaagtgtaag
     3961 cacggaaacg tttaagtcca ccagaacctg tacgtttgaa acgtttagct gatgcgcggt
     4021 gtgttttttg ttttggcatg attttttctc ctttatttaa ctttctgaca attatttttt
     4081 gtcagtcgct ggcgccaatt gcatgaacat ttgacgtcca tccattttag cacgttgttc
     4141 gatgatggca atatcttgag ttgcttcagc aaactcggct aaaacttttg caccaatctc
     4201 tttatgggta atcatacgac ccttaaagcg aatagatacc ttaactttat ttcctttttc
     4261 aaggaactta cgtgcattac gaagttttgt atcaaagtca cccttgtcaa tagttggact
     4321 tagacgaact tctttcacag taacaacgct ttgtttttta cgttgttctt tttgcttctt
     4381 ctggtactca aatttgaact taccgtagtc cataattttt gcaacaggcg gtttggcttg
     4441 gggttgaatc aatactaggt caacattagc gttatcagcc aaagcttgcg cttcactgag
     4501 tggcttgatg cctagctgtt ctccctcaag accaatcaag cgaacttcac gtacacgaat
     4561 ctcatcattg atgaataagt cttgctttgc tatggttttc acctcttttg ttttattaga
     4621 gaaaaacaat agcggactcg taaatataca agcccgcacg ttatgatagc gtttcttcga
     4681 aacttttcat ccgtagggcc aggcaactta atgtcacaag gcgagaagct ctcacttctg
     4741 cttttctcaa cttttatatg ataccaagtt ttctagctct tgtcaagata aaaagttatt
     4801 ttttgaaaag tttgtgttta tattttggtt tggttttcga atcaaaaaga gagaaccaag
     4861 ttgattctct ccctatgtta gtttttcttt agcttcccta taggaaaact aaactttaaa
     4921 aacttccttt ttacgtgaac ttccccatct ttcgataaat agaatcccca ctaacaaaag
     4981 gataatcagg caaggaagta agaccatccc catgctccaa tttagatcga cattatcaat
     5041 ctgcttagat aatcttccag ataaaatctc cactgaacgc aagtaagtaa agggaatcag
     5101 atgtgcaatc ctttgaagag gctgaattgt ttggatgcca aacaataagc caacaatccc
     5161 aatgagtgaa agaaagagga caggcatttt ttgcttgaaa aagtaagcaa tcaagtacac
     5221 aacttccaca atgacgataa aggctaagaa agctaagagc aagccaggaa ataatacatc
     5281 ttgtattttc ccaatagtta cttcttgatt cactaagcta taaattgggt agggataatc
     5341 taactgtcca aaaccactta tcagacttcc cactagaaaa gaaaagccac agattccgat
     5401 aaacagcaca gttacatatc ccactccaac tccaagagag gatattgcaa atgtcacttt
     5461 tgaaacagga tataagtgag ctgtgtccag atgattttga tatctttctg caaatagttg
     5521 tgttagcata aaaataattg caaccacaaa caagcttggg atgataacct ctaaaatcca
     5581 gacaatctga tcaatcccgt gggtcggaaa ctccaaagta tgtgctttta tgttcaaggg
     5641 atacagggct tggtaaatct tccgttcgcg gtcaaccccc atttttaagc cagagctagc
     5701 agtcgggtca tttgatacaa attcataatt cttctcttca tcttgccact gcagatagta
     5761 ggcctctttc cagcgccctt cttttaataa agtcagaatt tctgtctttc gcgtcaaaag
     5821 atttttttgc acgtctaaat tatttttagc aaactggtat tcctccgagc tggtatcaga
     5881 catttgggag agtttctctt cattttcatt gatagccctc tcgttggctg caatgcgact
     5941 ttccaacctg ctctccaagc tgtgtgagtt tgcagtctga ctatttaaat aaaaggtaac
     6001 accgagtaca gatgcaaata aaagtaagat aatccagttt aaacgacttt tgaaaacttt
     6061 tttcaataaa aatagactaa catctttcat aaactaaacc tcttctatct gcccctgatg
     6121 aatggttact actctatcgc agatatcaac caactcttcc ttatagtggg aacttaaaag
     6181 aaccagctgt tcttgtctat cgatttgtgc tagcctatca aaaaacttct gtcgataata
     6241 ctcgtctaag ccatttgtaa tctcatccat gagccagcat ttggcctgac tgagaaaata
     6301 catagcaatc accaagcgtt gcttcatgcc taaggaatac ttgcggatgg gaagactgat
     6361 atagtcagcc atttcccagt aggcgatttc atctctcaag tttaggtctg acttccagat
     6421 gttttttatg agacgaaggt agtccatccc acttaagttt ccatccagcc attcaacgct
     6481 ctcataataa aacaaagaag gaggaactgc gatatttcca ctacttatgg gaattaaatt
     6541 gctaatggca cggaacaggg tcgtctttcc agagccattg atagcaagaa gaccataaaa
     6601 cctacccttt ttaaaggtga aatccgcatc ttgcaagatg acttgtcgcg tttttaaggt
     6661 cacatgagta agctgcaaca tatctagccc tcctttttct cactctttaa gaattaatag
     6721 cctccgctgt aatagtttat gacctcataa cgagcgtagt tccagcctcc gccaacttta
     6781 tactcagaat agctgtaata acgagaccat tccggaatcc aagcatactg atggtcgtga
     6841 tagttgatac tatattccaa aaccgtattc caatcatact tgtaactttt agtggctgtc
     6901 acagcagata cactggactg aagaagacca atagattata aactaactaa taaaacaact
     6961 tttgctgatt tttaatgatt ttatatcctc aatattgaat acgt
//
LOCUS       DAVMHG010000107         6927 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMHG010000107 DAVMHG010000000
VERSION     DAVMHG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.109"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(117..392)
                     /locus_tag="VYR08_001657"
     CDS             complement(117..392)
                     /locus_tag="VYR08_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV3467155.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(498..1337)
                     /locus_tag="VYR08_001658"
     CDS             complement(498..1337)
                     /locus_tag="VYR08_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV3467156.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1479..2327
                     /locus_tag="VYR08_001659"
     CDS             1479..2327
                     /locus_tag="VYR08_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV3467157.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2420..3337)
                     /locus_tag="VYR08_001660"
     CDS             complement(2420..3337)
                     /locus_tag="VYR08_001660"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV3467158.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3775..3980
                     /locus_tag="VYR08_001661"
                     /pseudo
     CDS             3775..3980
                     /locus_tag="VYR08_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4291..4584)
                     /gene="rpmA"
                     /locus_tag="VYR08_001662"
     CDS             complement(4291..4584)
                     /gene="rpmA"
                     /locus_tag="VYR08_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV3467159.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4601..4945)
                     /locus_tag="VYR08_001663"
     CDS             complement(4601..4945)
                     /locus_tag="VYR08_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV3467160.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4961..5275)
                     /gene="rplU"
                     /locus_tag="VYR08_001664"
     CDS             complement(4961..5275)
                     /gene="rplU"
                     /locus_tag="VYR08_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV3467161.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5311..5398)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5463..6161)
                     /locus_tag="VYR08_001665"
     CDS             complement(5463..6161)
                     /locus_tag="VYR08_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV3467162.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     gene            6563..6862
                     /locus_tag="VYR08_001666"
     CDS             6563..6862
                     /locus_tag="VYR08_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV3467163.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2184 a         1402 c         1154 g         2187 t
ORIGIN      
        1 tgtacaatat tttcggaaca ttcaactttt taactctatt tattactaga tttcataatt
       61 aaaaagccta ctgaccaagc tagaaagctt gatacaatag gctttttaaa gactgattat
      121 ttaacagcgt ctttaagagc tttaccagct ttgaatgctg gtactttaga agctgcaatt
      181 gtcatttctt taccagtttg tgggttgcga cctttacgtt ctgcacgctc acgaacttca
      241 aagttaccaa aaccgatcaa ttgaactttt tcaccagctg caagatagtc agctactgct
      301 gcaaatacag cttcaactgc tgctgctgag tctttcttag tcaattctgt agcttctgct
      361 actttagcga tcaaatcttg tttgtttgcc atgttaatga ttcctccaga taatttctaa
      421 ttaacaaata taatcatatc ctaaaatccc ttttaggtca agttaaaaac gctatttctt
      481 ccaattttat ttatttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc
      541 gtgtgagttt gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct
      601 tgtaagcttt ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc
      661 tctgctacag cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt
      721 ttagttccac gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca
      781 cggatattga gaagggagct caacaagcca gttacacggc caattcgtcc acctttgaca
      841 agattttcca aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca
      901 tgagataaaa ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact
      961 tggaatttca gggcttggtc agtgaaggaa ctatcaacaa caatcacatc tgcagtagat
     1021 aggctagcac cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata
     1081 gcaagaatct ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca
     1141 ggtggctgac ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct
     1201 tcttctttca aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca
     1261 attgtaatat ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca
     1321 atcttaattt ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac
     1381 ttttaattat atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa
     1441 aatttgatat aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca
     1501 tttttaccat tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc
     1561 cccatcatct ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta
     1621 aaatccctgt ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga
     1681 agatctttgg agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccgcct
     1741 ggttagcttt ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca
     1801 cagcccttat agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag
     1861 gtacaactgg cggaactgat attctagctc gtattctcaa caaatacact catatatcca
     1921 taggaaaact gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca
     1981 aggatttgag attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg
     2041 atttgattgg tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg
     2101 accaacttgc taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc
     2161 aaggctacta tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa
     2221 ttgtgaaaac gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag
     2281 aagcccatga aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtaatgt
     2341 cgtgacctca aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt
     2401 gttttattta ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact
     2461 gatccactaa ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat
     2521 aaaccatgac ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac
     2581 gtgcttcttc tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct
     2641 gtctttgaat ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta
     2701 aaagcactaa attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacta
     2761 ccatacctct tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat
     2821 ctaaaatagc ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg
     2881 gaacaatgat aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag
     2941 aaccaaatgt ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag
     3001 ttgcaaagaa ttcttgtgca gtgagactag cgaattgact actaaaatca aggagatata
     3061 attcttctac accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca
     3121 aaaacaaatc tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa
     3181 cgacgatagg caataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat
     3241 gccccttatg tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa
     3301 tatctttttg gttttttata ggaatagtaa taatcataaa ataattatat catagcgata
     3361 gctatttctg gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt
     3421 caaaaagcac tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa
     3481 cttgtaggac atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aactataaag
     3541 tgtcatacta tgcttatgta tgaaaaagca atgcaactaa ctcctgagaa ctttaaatta
     3601 ctaattggtg ccgaaaaggt agaatttaga atagaggtac acctatggct gtaaaattta
     3661 caaaatgaga caacttgggc aagatgtttg aagaatttcc taaactccct gatttgaagc
     3721 aagtcacttt ccctgatgac aaagaaaaaa gccaaagcaa taaagaaaaa ctagatgact
     3781 gctttccaac aactcccatc tagtgtgctt cagactgggc tatttttctc tccatctgtt
     3841 agcttggatt ctcagaccgt ttcagctaaa gaatatcttt tcctttatca gaaggaacgg
     3901 ctcaagccat tcagacaagt gaagggacga caagccaata tttgaaacca gatagcagtt
     3961 cttatagtca attgaaataa aatctgaaga aatcgagtag gaaactcaaa tcaattttta
     4021 gcagtgttct attccagatt catactcaat gaaaattaaa gtgcaaacta ggaagttagc
     4081 cgcaggctgt acttgagtac ggcaaggtga aactgacgtg gtttgaattt gattttcgaa
     4141 gagtatcaat ccactataca tattaaaaag tatgggtatc aaaataaaaa aacaggctct
     4201 ccgaaaactc agaaagcctt attttaagct gtttctagtt tcctcacctt tacatttcaa
     4261 ggttcgggat aaaaggttca atggaccttt ttatttagcg attgggtaaa cagaaacttg
     4321 ttttttatcg cgtcctttac gttcaaagcg tactacgcct tcaactttag cgaacaaagt
     4381 atcgtctcca ccacgaccaa cgtttacacc tggatagatg tgtgtaccac gttgacggta
     4441 aaggattgat ccacctgtta cagtttgtcc gtcagctgct ttagctccaa gacgttttgc
     4501 ttgtgaatca cgtccgtttg atgtagaacc tccacctttt ttgtgggcga aaagttgcaa
     4561 gttgttaaga gtcattttta acataatgtt ttcctccgtg ttagttttct gtgataactc
     4621 tggtttggac gaactcagaa gagttctccg ataagtttgc catacctaag aaaaatgatt
     4681 caaagaataa ctgggtcatt tctctctggt gtgaaggaag atcttttggt atttcaacca
     4741 tcagatagcc accttcatct tcgtttaatt ctaggattgg ttcatagcct gcaaatttct
     4801 caatagaatt gataaagtta atggcaagcg tagaaaccga tgcacacacg acatctaagc
     4861 cgtattcgcc actctcggcg tgtccagtaa tttccgcact cctcagctcg ccatcttcgg
     4921 ctctctcaaa gactgcctgt atcatgtgtt ctccttaaaa ttaagcgttg attgcgttga
     4981 tgacaacttt tgtatatggt tgacggtgac cttgtttacg gtggctacct tttttaggtt
     5041 tgtacttgta agtaaccact ttcttttgtt ttccttgttt ttcaacagtt ccaactacag
     5101 tagctccagc aacaagtgga gttccgacaa cagtgttttc accaccaaca agaacaactt
     5161 cgttaaaagt aacttcttga ccagcttcaa cgttcaattt ttcaacgtaa actgcttgac
     5221 caacttcaac tttaacttgt tttccgccag ttttgataat tgcgtatgtg ctcattatgc
     5281 acctcctatg atttttatgg gtttccccga ttttttgtga agactcgcct agcatcgtgg
     5341 gacgaaccac ttaaacttga ataatcaagc aacgatgttc gtgcggttgc acaggatcgt
     5401 gcatagtcaa ctcttcaagt atagcatatc tcctattttc ttacaagtaa taacacctaa
     5461 aatcaagctt tttcttttac ttttttctgc caagaggcaa aaagcatgct gaggtaaaaa
     5521 acgctcatca taataggaac accaagaatg gtcttttcat gatagaaaat cgtcaaatag
     5581 gctgaaaaga caacgccaag gacaaaacta ctaagcaggc taacaaatat gaatccttca
     5641 cgcaaaaaag gagtgtgctt ggttcggaaa taatctccaa aagccagcat ggtccgtttg
     5701 atattccctg tcataaaagc gttattatag gcaatacccg acacttctcc aaaagcagtt
     5761 gtcaccagtc ccatacagaa ggccaagggc ggcactagat agatattatc cacagtttgc
     5821 ggcacaaaag caataatgat tgataagatt gccaagggaa tcaaggacag aataggtttt
     5881 ttcacaattc tcaatttttc cttataaatc gttaataaaa agactcccat cataaacgct
     5941 agcaaggtga gaaccttgtc cctaacatcc gaaacattat ttttaattaa ttctactgaa
     6001 agaaagacaa catttccagt ttgtccagct acaagggtat tcccgcgaac aataaaagtg
     6061 taagcatcca catatccagc acaaaacgtc aaaaaaagtg ctaacctttt agactgacgt
     6121 gatatttttc ttataggtaa taacctcatt ttacctccca ttgtattttc tcttagaaat
     6181 attgtaccat tttctttcta aaaaatcgta ggctaccatt tagattttac tattagcata
     6241 aaaataataa tagacaacta tttatccaaa aatagataga tgtaacatgt ttgcaaacaa
     6301 agcatacgaa cctttagtaa aatcatttcc atgaaactag aatagagccc tcttagcaaa
     6361 aatcattatt ttaatttatt tctaatcact ccttgacata aataactctc accaataaaa
     6421 gactatgtct taaaaaaatg gtataataaa atcaatactt gggcttgatg gctctgctac
     6481 taataacaat taggagagaa aatcaggcac ttgttaacaa caaggattat ccccttgaga
     6541 tgaaaggaac tttagaaatc ttatgatgaa catgcaaaac atgatgcgtc aagcacaaaa
     6601 acttcaaaaa caaatggaac aaagccaagc tgaacttgct gctatgcaat ttgttggcaa
     6661 atctgctcaa gatcttgtcc aagcgacctt aactggcgat aagaaagttg tcagcattga
     6721 tttcaatcca gctgtcgttg acccagagga ccttgagact ctttctgata tgaccgttca
     6781 agccatcaac tctgctcttg aacaaatcga tgaaactacc aagaaaaaac tgggtgcttt
     6841 cgctgggaaa ttacctttct aaaaacaagg agctagaaca atgcttgtcg ataacaaagg
     6901 ctaagaaagg tgcaaaaatt ataaaaa
//
LOCUS       DAVMHG010000108         6737 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMHG010000108 DAVMHG010000000
VERSION     DAVMHG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.110"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            48..731
                     /gene="rpiA"
                     /locus_tag="VYR08_001667"
     CDS             48..731
                     /gene="rpiA"
                     /locus_tag="VYR08_001667"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV3467164.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            745..1956
                     /locus_tag="VYR08_001668"
     CDS             745..1956
                     /locus_tag="VYR08_001668"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV3467165.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIYDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            1958..2500
                     /locus_tag="VYR08_001669"
     CDS             1958..2500
                     /locus_tag="VYR08_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV3467166.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            2516..3325
                     /locus_tag="VYR08_001670"
     CDS             2516..3325
                     /locus_tag="VYR08_001670"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3467167.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3822..3959
                     /locus_tag="VYR08_001671"
     CDS             3822..3959
                     /locus_tag="VYR08_001671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467168.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     T"
     gene            3978..4780
                     /locus_tag="VYR08_001672"
                     /pseudo
     CDS             3978..4780
                     /locus_tag="VYR08_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            4805..5317
                     /locus_tag="VYR08_001673"
     CDS             4805..5317
                     /locus_tag="VYR08_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV3467169.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFCFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGSGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            5971..6681
                     /gene="deoD"
                     /locus_tag="VYR08_001674"
     CDS             5971..6681
                     /gene="deoD"
                     /locus_tag="VYR08_001674"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV3467170.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
BASE COUNT         2058 a         1210 c         1558 g         1911 t
ORIGIN      
        1 agcggtttca tagtaaaata agaactgaga atgatggagg taaatcggtg gaaaatctga
       61 agaaaatggc aggtatcaag gctgctgagt tcgtgagcga tggaatggtc gttggacttg
      121 gaacaggctc gactgcctat tattttgtcg aagaaatcgg tcgtcgtatc aaggaagaag
      181 gattgcagat tacagctgta acaacttcta gtgtgaccac taaacaggca gaaggcctta
      241 atatcccgct caagtctatt gaccaagtag attttgtcga tgtgacggtt gatggcgcgg
      301 atgaagtgga tagtcagttt aatggaatca aaggcggtgg tggtgccctt ctcatggaaa
      361 aggtggtcgc aacaccatca aaagaataca tttgggtggt ggatgaaagc aagctggtcg
      421 aaaaactagg tgcttttaaa ttgccagtag aagtggttca gtatggtgca gagcaggtct
      481 ttcgtcattt tgaacgagct ggctacaaac caagtttccg tgaaaaagac ggccaacgtt
      541 ttgtgacgga tatgcagaat tttatcattg acctcgcctt ggatgtcatt gaaaatccaa
      601 ttgcttttgg acaagaattg gaccatgtcg ttggtgttgt ggagcatggt ttattcaacc
      661 aaatggtgga taaggtaatc gttgctggac gagatggagt tcagatttca acttcaaaaa
      721 aaggaaaata gaagggggca taagatgtct aaatttaatc gtattcattt ggtggtactg
      781 gattctgtag gaatcggtgc agcaccagat gctaataact ttgtcaatgc aggggttcca
      841 gatggagctt ctgacacact gggacacatt tcaaaaacag ttggtttgaa tgtcccaaac
      901 atggctaaaa taggtcttgg aaatattcct cgtgaaactc ctcttaagac tgtagcagct
      961 gaaagcaatc caactggata tgcaacaaaa ttagaggaag tatctcttgg taaggatact
     1021 atgactggac actgggaaat catgggactc aacattactg agcctttcga tactttctgg
     1081 aacggattcc cagaagaaat cctgacaaaa atcgaagaat tctcaggacg caaggttatt
     1141 cgtgaagcca acaaacctta ttcaggaacg gctgttatct atgattttgg accacgtcag
     1201 atggaaactg gagagttgat tatctatact tcagctgacc ctgttttgca gattgctgcc
     1261 cacgaagaca ttattccttt ggatgaattg taccgtatct gtgaatacgc tcgttcgatt
     1321 acccttgagc gtcctgccct tcttggtcgc atcattgctc gcccttatgt aggtgaacca
     1381 ggtaacttca ctcgtacggc aaaccgtcgt gacttggctg tatctccatt ttccccaact
     1441 gttttggata aattgaatga ggctggtatc gatacttatg ctgtgggtaa aatcaacgat
     1501 atctttaacg gtgctggtat caaccatgac atgggtcaca acaagtcaaa tagtcatgga
     1561 attgatacac tattgaagac tatgggactt gctgagtttg aaaaaggatt ctcattcaca
     1621 aacctagttg actttgatgc cctttacggc catcgtcgta atgctcacgg ttaccgtgat
     1681 tgcttgcatg agtttgatga acgcttacct gaaattatcg cagctatgag agagaatgac
     1741 cttctcttga ttactgcgga ccatggaaat gacccaacgt atgcaggaac ggatcacact
     1801 cgggaatata ttccattgtt ggcctatagc cctgccttta aaggaaatgg tctcattcca
     1861 gtaggacatt ttgcagatat ttcagcgact gttgccgata actttggtgt ggaaactgct
     1921 atgattgggg aaagtttctt agataaattg gtataagatg acgcgctatg ctttgctggt
     1981 gagaggtatc aatgttggtg gtaagaataa ggtcgtcatg gcggagcttc gtcaagaatt
     2041 gacaaacttg ggactggaaa aggttgagag ctacatcaat agtggcaata ttttctttac
     2101 ttcgatagat tccaaagccc aattggttga aaagctagag actttctttg cagtccatta
     2161 tccatttatt cagagctttt ctttactgag tctagaggac tttgaggcgg aacttgaaaa
     2221 tctaccagct tggtggagca gagacttggc acgaaaagat tttctctttt acactgaggg
     2281 tttggatgtg gaccaagtca tcgcgacagt tgaaagttta gagctgaaag atgaagtgct
     2341 ttattttgga aaacttggga ttttctgggg gaaattttct gaagaatcct attctaagac
     2401 tgcctatcat aagtacttgc tgaaggtgcc tttctaccgc cacattacta ttcgtaatgc
     2461 taaaaccttt gacaaaattg gtcaaatgct aaaaaaataa taaaggagac acacaatgac
     2521 atttttaaac aaaatccatg aaactgctac tttcctgaaa gaaaagggaa ttgcagcccc
     2581 tgagttcggt ctaatccttg gatcaggact tggagaattg gcagaagaaa tcgaaaatcc
     2641 agttgtagta gactatgctg agattccaaa ctggggccgt tcaacagtag tcggtcatgc
     2701 tggtaaattg gtatatggtg aactggcagg tcgcaaggtc ttggctcttc aagggcgttt
     2761 ccatttctat gaagggaatc ctctggaagt ggtgactttc ccagttcgtg tgatgaaagt
     2821 tcttggatgt gaaggtgtta ttgtaaccaa tgcagctggc ggtatcggat ttggtcctgg
     2881 taccttgatg gctatctcag accatatcaa catgacgggg caaaatccat tgatgggtga
     2941 aaacttggat gactttggcc cacgtttccc agatatgtct agggcctaca caccagaata
     3001 ccgtgccact gcccatgaag tggctaaaaa acttaatatc aagcttgatg aaggtgtcta
     3061 tatcggagtt actggtccga cttatgaaac accagcagaa attcgttcct ataagacact
     3121 gggagcagat gcagttggta tgtctacggt tcctgaagtt atcgtggcag cccactctgg
     3181 cttgaaagtt ctgggaattt catgtatcac taactttgcg gccggtttcc aagaagaact
     3241 caatcacgaa gaagttgtag aagtgactga acgtgttaaa ggtgatttca aaggcttgct
     3301 taaagcgatt cttgctgaat tgtaagaaaa aagatttaaa agggggagtg cctctgtttt
     3361 ttcaggattg actgcctatc cggattaaag aagaaacaga ggaatactat gagcttcttc
     3421 ctgctcttat aactgaaaga agcggaagaa taggtatgtc tgatctgata gccagcattg
     3481 tgaaagacaa gattctagga tactagcatt agcttcctag ccaagcagac tagtatgata
     3541 aggagagatg agaatgaatt gactttctga atttctcagt cttatcatat atagcacaat
     3601 gagatttcgc ttgagtctgc ttgtaaataa acgaaaagaa agataagaaa taatgaaaat
     3661 tggtcaacga attatgcgct ttggcataaa aaattaagta tcggagttgt atctgttgta
     3721 gtcggctttg atttctagct ccagctggaa tttcagccaa tgaagtaaag caagatgtaa
     3781 catctgaagt ggtaataggt gtgctagatt ctaaggagga attgaaagag tcagaaaatg
     3841 atgctccaaa actagaaact cctcttagag aggagccaag actagctcct caaacgcttc
     3901 cggaagcaag tgaagttctt gaaaacaaaa gggaagagtc aaaagtagag ataacataac
     3961 cagctcaagc ggatgatatc cgcaaggttg ttggggaatt agccaaggat ataagtatta
     4021 ctaagttgta tatgacaggt cattctcttg gatgttacct agctcagatt gcagcggttg
     4081 aagcttacca aaaatatcct gatttttata accatgtatt gaggaaagtg acaactttca
     4141 gtgctcctaa agtgattact tccagaactg tttggaatgc taagaatggt ttctgggatg
     4201 ttggtttgga aagtcgtaaa ttagctgtta gtggaaaaat taagcattat gtggttgata
     4261 atgacaatgt tgtgactccc ttgattcata ataatcgtga tattgttaca tttacaggta
     4321 attcacgctt taaacaccgt tctcgtggct attttgaaag tccaatgaat gatattccta
     4381 actttaatat tggtaaacaa gctaccttgg ataaacatgg ttatcgtgat ccgaaattgg
     4441 ataaagtgcg attctttaag aaacaggctc tgcctcgatc ttctagtcaa ccaagcgctg
     4501 aaccaatgga aaatattgcc tcaggaaaac aggttactca aagttcgaca gctttcggag
     4561 gagatgctag aagagctgtg gatggcaaag tcgatggtaa ctatggtcac aattctgtca
     4621 ctcatacaaa cttccaatct aagccttggt ggcaagtaga tttggtaaag aagaaaccat
     4681 tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg caaactttga
     4741 tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa catctcctaa
     4801 agatgtgtca gcacaaatta cgattaacca taaaaaagcg cgctatgttc ggattgagct
     4861 agaaggctat aatgccctca gtcttgcaga agttgaagtt ttctgcttta tagctacgaa
     4921 tgctgaaacg gcgacacaag tttctaagcc agttcaacca atcagtcaga ctcctgtgaa
     4981 ggataaaaca ttgacaattc aacacagtgg agcttacatt gcccgctact ccataacttg
     5041 ggaagaagtt ccagtagata aagatggaaa ccaagttgtt cgtagtcatt cttgggaagg
     5101 aagcggtcgc aaccagactg caggttttgt cctcaacctc ccaatcaaag aaaatatgag
     5161 aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat ggcaaacaat
     5221 ctatgaaaac agaccaattt tagctcaacc ccaccgtaaa attacccatt ggggtacgac
     5281 attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtagaa tgacagttag
     5341 tttgtctagt ttataagaaa gtactacctg agcttgaata ggactcaggt agctctctat
     5401 gaaagaacaa aattaatact caatgaaaat caaagagcaa actaagaaac tagccgcagg
     5461 ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc acatatataa
     5521 tccaaggcga cgttgacgtg gtttgaagag attttcgaag agtataaaca gaaaggtaga
     5581 gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaaacaaaaa cgaaaggatg
     5641 ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa aattaagaaa
     5701 ggaattgacc ccaccctaaa agtagtggga aaaagatagt tgatctagcg agcatcgctc
     5761 actgcgccca actcctattt tcccttcgct ttttgatggg tttggtatct ttctcaatat
     5821 aaaatataaa ataaagaaag gtagagcgtg tgttttgatt tgaacacgag cggaaaactc
     5881 ggaaaataga taatctgact gaaaaatcag gatttctcgt caggttccta attttcagtc
     5941 gttttcttct cgctctttgt atcataaatt atgtctatcc atattgctgc tcagcagggt
     6001 gaaattgctg ataaaattct tcttcctggg gatcctcttc gtgctaagtt tattgcggag
     6061 aatttccttg atgatgctgt ttgttttaac gaagtgcgta acatgtttgg ttacactggt
     6121 acttacaagg gtcactgtgt atctgtcatg ggaactggga tgggaatgcc atctatttcg
     6181 atttatgcgc gtgagttaat cgtagactac ggtgtgaaga aattgattcg tgtgggaact
     6241 gcaggttctt tgaatgaaga ggttcatgtt cgtgaattag ttttggcgca ggcggctgca
     6301 accaactcaa acatcgttcg taatgactgg ccacagtacg attttccaca aattgctagc
     6361 tttgatttgc ttgataaagc ctaccatatc gccaaaaaac ttggtatgac tactcacgtt
     6421 gggaacgttt tgtcatctga tgtcttttac tcaaattact ttgaaaagaa tatcgagctt
     6481 ggtaaatggg gagtcaaggc tgtggaaatg gaagcagcag ctctttacta tcttgctgcc
     6541 caataccatg ttgatgcgct agctatcatg accatctctg atagcttggt caatccagac
     6601 gaagacacaa ctgcagaaga acgtcaaaat accttcactg atatgatgaa ggttggtttg
     6661 gaaacattga ttgcagaata attatagcca aaaaggggct ctttgtcaac tgtagtgggt
     6721 tgaaaaaaag ctaagct
//
LOCUS       DAVMHG010000109         6731 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMHG010000109 DAVMHG010000000
VERSION     DAVMHG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.111"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            46..119
                     /locus_tag="VYR08_001675"
     tRNA            46..119
                     /locus_tag="VYR08_001675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Asn,seq:gtt)
     gene            complement(160..702)
                     /locus_tag="VYR08_001676"
     CDS             complement(160..702)
                     /locus_tag="VYR08_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV3467171.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            860..1627
                     /locus_tag="VYR08_001677"
     CDS             860..1627
                     /locus_tag="VYR08_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEV3467172.1"
                     /translation="MIGISLIPDLYNIIFLSLMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            1712..1987
                     /locus_tag="VYR08_001678"
     CDS             1712..1987
                     /locus_tag="VYR08_001678"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467173.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            2077..2391
                     /locus_tag="VYR08_001679"
     CDS             2077..2391
                     /locus_tag="VYR08_001679"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV3467174.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(2492..5044)
                     /locus_tag="VYR08_001680"
     CDS             complement(2492..5044)
                     /locus_tag="VYR08_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV3467175.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5106..6728)
                     /locus_tag="VYR08_001681"
     CDS             complement(5106..6728)
                     /locus_tag="VYR08_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467176.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2224 a         1109 c         1243 g         2155 t
ORIGIN      
        1 tttcacggcg gtaacacggg ttcgaatccc gtacggacta ttttatccgc catagctcag
       61 ttggtagtag cgcatgactg ttaatcatga tgtcgtaggt tcgagtccta ctggcggagt
      121 atagataaaa gggaacacag ctgtgttcct ctttttgtat caatttgtat caccaagcat
      181 tttcataagg aagtctgtta tttcttgaga actttctttt tttccatgtg caatccaagt
      241 ttggcagaca ccaaaaagtg catgagttag atagatgcta ctatattcta attcggtggt
      301 atttagattc agttgcataa atcgcttttg taaatctgta ctaagcatga tatgaagttt
      361 atttcgtaag aaattttgga tttctttagt cccattttca gaaagaaggg cagccagaag
      421 tggttctgac tctagatatt caaaaacttc taaaatagcg tctcttttgt gatgagcatg
      481 tttttgaaaa atatattcaa atgtatggaa tagcttgctt tgatagtgct caatcatatc
      541 atacttatcc ttatagtgag tatagaagct ggaacgacta attccggctt tttctactaa
      601 tttgacagta gaaattttat caaatggctg ttccatcagt aattgtacca tagcattttc
      661 aatagttcgc tttgttttta agcgtttgtt actttcttgc atatttcctc cttgtaaaca
      721 aattagacta tatgtctaaa aatagatttt ttaatgtata atctattata tcaaaatttt
      781 agacaatatg tttaaaaaag gagaaactaa gtttaaagaa tggaaagcaa tttaaaaaaa
      841 accaaccttt attattgtca tgatcgggat ttctcttatt ccagatctgt acaatatcat
      901 atttttgtca ttaatgtggg atccatatgg gcaattgtct gacttacctg tggcagttgt
      961 aaataatgat aaagaggctt cctataatgg taatactatg gcaataggaa aagacatggt
     1021 gtccaattta aaagaaaata aaaccttgga ttttcatttt gtagatgaag aggaaggaaa
     1081 gaagggattg gaagatggcg attactatat ggtagtgact ttaccaagtg atttatctga
     1141 aaaaacaact acattatcca atattcaatc gacagcagct tatcaatcat tgacaagtga
     1201 gcaacaaact gagataagtg attctgtatc tcaaaattca actgatagta ttcaatcggc
     1261 tcagtcaatt gtagctttag tacaagattt acagggaagt ttagaaaact tacaaaatca
     1321 atcttctaat ctttcgactt taaaaaatca atctaatcaa gtatcaccta ttacttctac
     1381 ttctttgata ggattgtcaa gtggattaac agagatacaa ggagatgtta ctagcaaatt
     1441 agttcctgcc agtcagtcga ttgcatcagg tgtaaacgca tatactacag gtgttgataa
     1501 agtttctcag ggcgcaagtc aactaagtga aaaaaatgcc accttgacag gtagtttgga
     1561 taaactagtt tcaggctcaa acaccttgac acaaaaatct tctagattga cagcaggagt
     1621 tggttaatta caatcaggat ctgggcaatt agcagacaaa tccagtcagt tactttcagg
     1681 tgcttctcca ttagagaata gagctaataa attggcagat ggatctggga aactagcaga
     1741 aggtggaaca aagttaactt ctggattgga agatttacag acaggacttg cttctttagg
     1801 acaaggacta ggtaatgcta gtgatcaact caaatcagta tcaacagaat ctaaaaatgc
     1861 agagattttg tcaaatccac tcaatctttc aaaaacagac aatgatcaag ttcctgtaaa
     1921 tggaatcgca atagctcctt atatgatatc agttgctctt tttttgcagc aatatcaaca
     1981 aatatgatat ttgcgaaatt gccttcagga cgtcatccag agagccgttg ggcttggttg
     2041 aaatcttgag ctgaaataaa tggtattata gctgttttgg caggaatttt ggtatatgga
     2101 ggagttcagc ttattggttt aactgctaat catgagatga gaatatttat tctcatcatc
     2161 ctaacaagtt tagtattcat gtctatggtg accactttag caacgtggaa tagccgtata
     2221 ggagcttttt tctcacttat tttgctttta ctacagttag catcaagtgc aggtacttat
     2281 ccacttgctt tgacaaatga tttctttaga tctattaatc cctggttacc aatgagctat
     2341 tcagtttcgg gattacgaca aacaatctct atcaacaagt cattttccta gctgtcatac
     2401 tagttctatt tactagttta ggtatgctag cctatcaaca taagaaaatg gaagaagatt
     2461 aaaaaaatcg accgattaac tggtcgattt tttatgcctt agatgacttt cgtctgtgat
     2521 tatagattcc aaatagtaag agagaagtaa aggaacagat tgctccagta ataaaaccat
     2581 tgggaatgaa ggaaagtgta atagttcctt tccccttggg aatgtcaact ttcataaatc
     2641 cagtttgagc ttgtttaatt tctattttct taccatcttg gtaggcagac caacctttgt
     2701 cataaggaat ggtgaagaaa atagatgtat cttgttggac atcatatgta gcaaaaacct
     2761 tgtttttaga agttgatact gtgacaggtt gttctttaat tttttgaatt gcctcggtga
     2821 aagttttggt atctaaacga tagaaggtag gagattcaaa tgatacttgt gaatttccag
     2881 ggaaactaac attgatattg aaagtttttt tctctttagt atatcctaga ttaaagaagg
     2941 agaagacatt atcagttgta aaagtctttt tttcaccatt tacaaggatg tcaaccttct
     3001 tttgtttatc gttagaaaag tgaaggttta tgaaagagag ataaacttgg ctgttttctg
     3061 gaacttcaat ttgatactgg attgctgcat cttcatttga agaacttgtg acactaatca
     3121 aatcattagt attttctatt ttttctgttt tttcataagg tattggagaa aaataatcaa
     3181 aattgacgtt agcaagttga tttaaaaatg aggcctgatt atccaaggta tgttcattga
     3241 acttgacatc attgtaaaca gattgactcg caactgcaat cggaagagag tattgatttt
     3301 catatagggt aagattatct ttttgataga tatctttaaa gccatactta tcaataggac
     3361 tgtctgagat attgtactgg ataccaaata aactatcagc caaaatacta ttatttgcat
     3421 atcggagatt gagattagtc ccagaggatt taaaaccaag tttatctaaa gtagagcttg
     3481 atgaacgatt tcgaacagat gaaaattgag agattccatt gtagttgaat ttcatactgt
     3541 catttcctgt ctgagtttgt agtttttcag tacgagtaaa ttgatttcca atatatgttg
     3601 agaaagattc catagctggg atatctcgac tataagcact tcgagaagca aatccccatt
     3661 ccttagcaat tccgtccatt tgagatgaag catttaaact catttcaacc agtataaata
     3721 aagagattag aatggcaaat agattcacag atataaactt tttgataact gcaaggagta
     3781 aaagagaata gacaaccaaa aattcaagag taagcagaat attcaaatct gttaaaaaag
     3841 aataatgcga ttttagatag atggtagcta aaaatcctgc tactacaaga aaaagcgaaa
     3901 ctaaaaaatt ccagacttta agttctttca gacgctttaa gacttctgct gctgtgtaaa
     3961 ttaacaaggt agagaaaatc caagcatagc gatgtaaaaa catgtttgga gtatgcatgc
     4021 cttgccaaaa taagtcaaga gcttctatgt aaaagcttgc aattagaaat gcaaagaata
     4081 ttacatatat gagtttcacg tgaaacttaa tagatttcag cgtaaaaaat aaaatggtca
     4141 aaataaaggg aaatagtcca acaaaaatca ttgggatggc cccatacttt gttgtgtcaa
     4201 aggaaccaat gaattgctta gcaaagagat caagatacca gctactttca gtttgaaact
     4261 ttgtaacttc agtcaatttt tccccatgtg tctgtaaatc aaatagagtg ggaagagtca
     4321 taatcaaact agccatacca gctaaaaagg agataactat gaaatcaaga acagatgatt
     4381 ttcgagtctt aaagtcccac gaaatttgac agagatacca gaaaataaga aacaatactg
     4441 tcatatatcc aaaataataa ttttgaataa ataagattga cagacttgta aagtacaata
     4501 ggagtttctt ttcagttatc agtagatgta aaccagttat aattaaagga atcaagataa
     4561 aaacatctag ccaggttttt atctctaatt gactgacagt gaaactcatc agagcatagg
     4621 aagtagataa ggctagtttt aaaatctgag ggatagattg aaacaattta ttcaaactaa
     4681 aaaaggttga cagaccaatc aatccaaatt ttaagagagt tgtcagatag atagcatctg
     4741 gcatattcgt tagatcaaaa aagtaaacca gaggcgcgag aaaactaccc aagtaataac
     4801 tagatagggc atagaagttt agccctagac cacttgtaaa ggtgtaaaac agattactat
     4861 ttccatgtag gatatttcgt aaggctacat caaaaataac gtattgatga aagccatctc
     4921 ctaatagagg agagttgtcg ctattccagt agataccttg agatagatat actccagaca
     4981 taatcactac aggaatgatg aaagaaataa aataggttcg atatgttttt aaaaatgatt
     5041 tcatgttacc tcgtagaatg atagaaaact cagttggtta acccaactga gttttgaagt
     5101 tttatttagt ctttccaaag ttctttaact tttgcttgta cttctgcatt ttctaggaat
     5161 tcatcgtagg tttcatcgat acggtcaatg acgccatttt tagataagac aatgatatgg
     5221 ttagccaaag tttgaataaa ttcgtggtca tggctggcaa agatgattga ttctttaaag
     5281 tttttcaatc catcattcaa gcttgagata gattccaagt ccaagtgatt tgttggatca
     5341 tcaagtacaa ggacatttga ttttaagagc atgagttttg aaagcatgac acgaactttt
     5401 tctccccctg acaagacatt tacaggtttg ttaacttcat ctccagagaa gagcatacgg
     5461 ccgaggaagc cacgtaggaa agtattgtca tcttcttctt tacttgcgaa ttgacgcaac
     5521 cagtcaagaa ttgattctcc tcctgcaaaa tcagctgagt tatcttttgg taggtaagat
     5581 tgactagttg taactcccca cttgacagtt ccttcatagt caatatctcc catgattgca
     5641 cgaattaatg cagtcgtttg aatatcattt tgtccaataa gtgctgtctt atcatctgga
     5701 cgcaagatga aactaatatt atccaagata gtttcaccat caatctttac agttaaattt
     5761 tctactgtca agagatcatt accaatctca cgttccgctt taaagttgat aaatggatat
     5821 ttacgactag atggcacaat ctcttctagc tcaatcttat caagcatttt cttacgtgat
     5881 gttgcctgcc ttgacttaga agcattggca gagaaacgag caacaaattc ttgcaattgt
     5941 ttaatttttt cttctgcttt agcattacgg tctgctagca atttagcagc aagctcagaa
     6001 gattccttcc agaagtcgta gtttccgaca tagagtttga tttttccaaa gtcaaggtcg
     6061 gccatgtgag tacaaacttt gtttaagaag tgacggtcgt gggatactac gataactgtg
     6121 ttatcaaagt caatcaagaa gtcttctaac caagtaatcg attggatatc caaaccgtta
     6181 gtaggctcgt ccaagagaag aacatctggt ttaccaaaaa gtgctttggc gaggagaacc
     6241 tttacttttt caccgttggc caattcgctc atgttttggt agtgtaattc ttctggaatg
     6301 tttaggtttt gaagtagttg agaggcttca ctctctgctt cccaacctcc aagttcggca
     6361 aactctcctt cgagttcggc agcacgaacc ccgtcctcgt ctgagaaatc ttccttcatg
     6421 tagatagcat ctttctcttt catgatgcta taaagttttt catttcccat gataacgaca
     6481 tcaatggcac gttcatcttc gtagtcaaag tgattttgac gaagaacaga gagacgttca
     6541 tctggaccaa gagagatgtg accagtagta ggttcgatat ctccagctaa aatttttaaa
     6601 aaggttgatt ttccggcacc attagcaccg attaatccgt aagtatttcc ttctgtaaat
     6661 ttgatattga catcatcaaa aagtttgcga tcactaaaac gtagtgaaac atcagatact
     6721 gtaagcaatg t
//
LOCUS       DAVMHG010000110         6708 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMHG010000110 DAVMHG010000000
VERSION     DAVMHG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.112"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      13..111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            195..1001
                     /gene="thiD"
                     /locus_tag="VYR08_001682"
     CDS             195..1001
                     /gene="thiD"
                     /locus_tag="VYR08_001682"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV3467177.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIHPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCT
                     FAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1211..1843)
                     /locus_tag="VYR08_001683"
     CDS             complement(1211..1843)
                     /locus_tag="VYR08_001683"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3467178.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMDTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1836..2639)
                     /locus_tag="VYR08_001684"
     CDS             complement(1836..2639)
                     /locus_tag="VYR08_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3467179.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2639..3163)
                     /gene="thiW"
                     /locus_tag="VYR08_001685"
     CDS             complement(2639..3163)
                     /gene="thiW"
                     /locus_tag="VYR08_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV3467180.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(3168..3860)
                     /gene="tenA"
                     /locus_tag="VYR08_001686"
     CDS             complement(3168..3860)
                     /gene="tenA"
                     /locus_tag="VYR08_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV3467181.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKVFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3871..4521)
                     /locus_tag="VYR08_001687"
     CDS             complement(3871..4521)
                     /locus_tag="VYR08_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV3467182.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4523..5908)
                     /locus_tag="VYR08_001688"
     CDS             complement(4523..5908)
                     /locus_tag="VYR08_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467183.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5909..6469)
                     /locus_tag="VYR08_001689"
     CDS             complement(5909..6469)
                     /locus_tag="VYR08_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV3467184.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2085 a         1453 c         1316 g         1854 t
ORIGIN      
        1 aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc
       61 ctctgactca atctagataa tgctagctga tggaagtgga aatgataatg gggactagca
      121 gtcttctatt gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg
      181 ttgggaggtt ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta
      241 gtggtggtgc tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa
      301 tggctgttgt aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg
      361 tttctcctca aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg
      421 ctgtaaaaac tggaatgttg gctactactg aaatcatgga aatcatccac ccctatctta
      481 aaaaactgga ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct
      541 tgattgactc aagtgctaga gactatctca aaacaaactt actacctcta gcaactatta
      601 ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag
      661 acatgcagcg tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca
      721 aaggcggaca tctcaaaggc ggacatctca aaggtggtgc taaagatttc ctctttacca
      781 agaatgaaca atttgtctgg gaaagcccac gaattcaaac ctgtcacacc catggtactg
      841 gatgtacctt tgctgcagtg attactgctg aactagccaa gggcaagagt ctttaccagg
      901 cagttgataa ggccaaggcc tttatcacaa aagctattca agatgcccct caactcggtc
      961 atggttctgg tccagtcaac catacaactt ttaaagatta agaaaaaaaa ctctctagtt
     1021 cccactttaa gggaattaga gagtttttat actcttcgaa aatctcttca aactacgtca
     1081 gcttccatct gcaacttcaa aacactgttt tgagctgact tcgtcagtct tatctaaaac
     1141 ctcaaggcag tactttgagc aacctgcgac tagctttcta gtttactctt tgattttcat
     1201 tgagtattaa ttaggaaaga atgtcatgca actttgttaa aaaggcttgc gtttttgcct
     1261 caatatcttc tgcttgcatc aaatcacgta caacagctac accagctatg ccagtgtcca
     1321 taagctgatc aatattctct gatgtcaagc cgccaatagc aactactgga atggcaaccg
     1381 tttggcaaat tgttttcaag gtcgatatca gagtaatggg cgcattttcc ttggtggtgg
     1441 ttgggaaaat ggctcctgta cccaagtaat ctgcacctga tttctccgct tccagagctc
     1501 ttttaaccgt tttagcggtg acaccgagga ttttttcagg acccaagact ttgcgagcta
     1561 ccgaaactgg taattcatca tctccgatat gcagacctgc tgcatcaacc gcaagacaaa
     1621 catccaaccg atcatcgatt atcaagggta cctgataagc atctgttatt tccttgactt
     1681 gttttgccag ttgataatat tgattggttg tgagattttt ttctcgcaat tggactatgg
     1741 taacccctga acggcaggcc gtctcaactt ttgcaagaaa gctttccacg gaatcttgat
     1801 agcgattggt taccagatat agtctaagtg cttctctatt cataaacctc tcctttgatg
     1861 gtatctagcc aattttcatc tcttcttagg agcgaaagct gattgagtac ttggtaacga
     1921 aattcttcca atcccattcc ttgaacaact attttctcag cagcgatatt gagataagag
     1981 actgctaagc aagaagcttc aaaaccagtc tttccttggc tgagaaaaac ggctgttaag
     2041 gctccaacca aatctcctgt ccctgttatc cagtctaatt ctgcacagcc attctcaagt
     2101 acagcaactt gattctccga aacaataagg tccttgggac ctgtgactaa gaatgacata
     2161 ccaggatagg tctgacacca gtctttcaag acttgaagca aatcctccgt ttcttgatct
     2221 ttagcactcg catcgacccc aacgccgtga tgctttaatc caacaagact tcgaatttct
     2281 gacatgtttc ctttaaggac cgtaggtcta tagtctaaaa ggtctttaac taagctctta
     2341 cgaatggatg aagtcgttac gccaaccgca tctactacca tcgggagaga agattggttt
     2401 gcatacaaag ctgccatgcg gattgctttt tccttctcag ctgacaaatg ccccaaattg
     2461 atgaagagag cctggctttg cttagtaaaa tcaagaactt cacggggatc atctgccatg
     2521 acaggtttgc atcccagagc caaaatccca tttgccagca tctcacaaga aatctcattg
     2581 gtcatacagt gaatgaggga actagagcct ataggaaaag gatttgtcaa tgcctgcatc
     2641 attctatcct ttcagcaaag aaatatcctt gcactttttt aaagaattcc tgcttgatta
     2701 aaaatctaaa tgcaataaag gaaatcgctg taccaatcaa ggttgctccg aaaaatcgag
     2761 gagtgtagat aaaccaacta agcttagcag ccgatcctgt aaagagcacc ataacaggat
     2821 aggaaacaat agaaccaata atacctgttc ccacaatttc tcccaaggca gaaaagtaaa
     2881 attttcgacc gtacttataa aagagacctg ctagaagggc tccaaaagtc gctcctgtga
     2941 gagctaaagg cggaatccct tgagtcgtca tacggataaa ggctgtcact gtagccatag
     3001 ccaaggcata aacaggtccc atcatgattc ccgctagaat attgactaca ctggacatcg
     3061 gtgccattcc ctcaatccga aagataggtg taaggactac atcaagggca atcatcatag
     3121 ataaaatggt caatttgtga acttgtagtt ggtgctttct catgtttcta ttcttctcct
     3181 ttttctaaag actgtaaatc gctcttccat gtctggtgtt ggtaagccat ctcccaaaac
     3241 ttggcttcca tatgaacact gatgtggaag gcatctagca ttttttgctt atctgtctca
     3301 tcactttctc gatagagctg attgaccaat gctccctcct ctctgatctg ttgctctaac
     3361 tcatccgtaa tataagtttc aatccattgt tgatagagag gatttggtga tggtttaaga
     3421 ttaagtgatt tgcctatatc atggtataac caaggacaag gaagcaagct tgcaaaagct
     3481 atggctaagt tcggttctgc aaattgccta taaatatgag aaatgtaatg ataacaggtt
     3541 ggagcgattg gatgttgctc catttcctgg tcgttgattt ccaattcctt gaaaaattgt
     3601 tggcgaataa ataactcacc ctccactaaa ccctgagcat tttgtttcaa gagtcttttc
     3661 atctcttggt ttgaagtctt atcagccaaa agatgataga tttctgagaa aaccttcaga
     3721 tagtaggcat cctgaatcag gtaatagcgg aaaatggcag gttctaaatt cccctcttgt
     3781 aattgtaaaa taaagggatg atgaaaggaa gcctgccaag ctttcttgga taattccatc
     3841 gcaatatctg taaattccat aataactcct ttataaaaat agactggttt gaagcaataa
     3901 aaagaaaagc aggtagatta attttgtttt tttaggaata taaaaagtcc gatagctatt
     3961 cttcaactgt gcatgttcgt catatccgtg agcagataga gctctcaggt aaagatggcg
     4021 ccacctaaag actgtcatca gaaccttact gtaaatcaag ggcgaccaaa aatgtagttc
     4081 ttgaccacgt aataggcaag cttctttgag ggacttgatt tcttgctgaa tgagaggaaa
     4141 agaattgaat accacaatca aggcatagga ccaagagcgt gatagcccct tttgagccaa
     4201 gtacaagaga agctctttta gtgaaataga ggaaacaaag acaaggccga tacaaactgt
     4261 cacaaaggcc ctcgttccaa gcatgactgc ctgtgaagca tctccgtgta actgaactgc
     4321 ccagtagttg gcaaaagatg gtaaaatggc aagtatgatc atccaagcta acattttaaa
     4381 tcgacggtaa tagagcataa agagaataca aaatgcgact accgaaagat tcagagcaat
     4441 cgaaggaatg aaagatgttt ccaaggataa aatcagcaag aagagactga taatcggtgt
     4501 ctgggttgct actttgacca tactatctca cctccccttg ggtattgcta ctctgagatg
     4561 taagtggttt ggtaatggtc acttctttca catgccgaag accctgacta gtcatctcaa
     4621 tccaataatc aaccacagaa atcaaagggt ctaaacgatg actaatgagc agaaaacttc
     4681 ttccttgatt cctctcctcc acaatccact tgcaaaaata atggcaggct ctatcatcca
     4741 aacctgcaaa aggttcatct agcaagatca cggaagcctt actggtcaag atggtcagga
     4801 gctgaagaat tttttgctga ccaccactta attgataggg actcttatcg actgcctgct
     4861 ccaaatcaaa atatcgtaaa gcttgaaaaa tccgctgatt tctttcagaa tcaggtccat
     4921 ctaattgaag ctcctctcgc agactgactc ggataaactg cttctcagct tcctgaacaa
     4981 caccagtcag atcacgatac aaactctttt tctttttcag gaccgaaccc ttccatgcaa
     5041 tgcgcccctt atacttttga aattgaagaa tagaccgaaa gagggttgat ttcccgacac
     5101 cattgtcacc caggatacag gaaatccctt gatagaatgt gaaatcagca attgaaaaga
     5161 gggggcgatt tccaaactca caagtcgttc tatccatatg gaatagttcc gggctagaag
     5221 caacttcctt tgaagcaacc tgtgtcatct cataggaagg gatttggaac acttccctta
     5281 gttttccgtc tcttagctca accatatggt cgatataggc tttatagtca gataaatcat
     5341 ggtcgcacaa aataactgtc tttccatcaa gagccaactc ttttagaatc tccaatatct
     5401 cgattctgct cttgcggtca atggaagcaa aaggttcatc caagagatag accctaggat
     5461 tcatggcaaa gaggacagcc agcgctgctt tttgcttttc cccacctgat aggtgatgaa
     5521 tgggacggtg taagattgcc tcgcaacgac attgctggac aacctctgct attttagaat
     5581 caatttcctg aaggtgatag ccgatattct ccaaggtgaa aatcagctcc tcaaacaagc
     5641 tctccatggt aaattgatga ttgggatttt gaaagagaat gccaaccttc tggacacgtg
     5701 cgatgataga aagttgactg acctcgctcc catctatcag gacttgacca ctatagggaa
     5761 gagaactgac ttgagcaatg acttgaaaaa ggctggattt tcctgaacca ctgctcccaa
     5821 ctaacaaggt aaaggcttgc gcatgaaaag taaaatcaaa cggctcagag aagattgggg
     5881 actgaatcgc tcgtagttcc agacccatct atgcttttcc tccagttgca aactgatgat
     5941 agagtttgac aatggcacga accaagatgg tacagaagaa ataaacagaa ataaaacgta
     6001 ccacaagtaa ggaaaggaca aacggaaggg aaaaggcgta gtaacctaac ttaatgtatt
     6061 catagacaaa gctaacaagc gtaatcccaa tactattagc agttagagag agccaacttt
     6121 catagcgatt cttagttacg ataaaaccaa attcacttcc caaaccttga accaagccag
     6181 acaaaagagc tcctagacca aattggctac cataaaggac ttcagcaagc gcagctagca
     6241 cttctccaat cgttgcactt ccgactctcg gaacaaagat ggcagcaatg ggcgcagcca
     6301 tacaccagag accgaagagg atttcattgg caaaggcctg caaaccaaga ggtgttaaga
     6361 gtagactgag aatattatac acatatcctg aaccaacgaa aaccccacca aaaaagatag
     6421 acaagaaagc aagcaagata acatctttta actgccattt tttcaacata aaaaactcct
     6481 ttttttaaag aaaagtgggg cactcaagaa gaccgaccta aatactttgt atagcagact
     6541 gaatttagaa cagtacacaa gaatgctaaa atatttctag aaattaattt gaattttcta
     6601 attgattcgt tcgcatctta tttcaatcta ctatagaaaa aagtctttaa aattataaaa
     6661 cgcatcatat caaggttttt caaaaacctt gatatgatgc gttttatt
//
LOCUS       DAVMHG010000111         6655 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMHG010000111 DAVMHG010000000
VERSION     DAVMHG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.113"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      complement(84..172)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(381..1010)
                     /locus_tag="VYR08_001690"
     CDS             complement(381..1010)
                     /locus_tag="VYR08_001690"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV3467185.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALRGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1012..1794)
                     /gene="thiM"
                     /locus_tag="VYR08_001691"
     CDS             complement(1012..1794)
                     /gene="thiM"
                     /locus_tag="VYR08_001691"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV3467186.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            1878..1970
                     /locus_tag="VYR08_001692"
                     /pseudo
     CDS             1878..1970
                     /locus_tag="VYR08_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2120..2788)
                     /locus_tag="VYR08_001693"
     CDS             complement(2120..2788)
                     /locus_tag="VYR08_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV3467187.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(3003..3091)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3345..4481)
                     /gene="lctO"
                     /locus_tag="VYR08_001694"
     CDS             complement(3345..4481)
                     /gene="lctO"
                     /locus_tag="VYR08_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV3467188.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(5105..6595)
                     /gene="lysS"
                     /locus_tag="VYR08_001695"
     CDS             complement(5105..6595)
                     /gene="lysS"
                     /locus_tag="VYR08_001695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV3467189.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT         1981 a         1438 c         1130 g         2106 t
ORIGIN      
        1 agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc ttacaagatt
       61 agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc agtcttaact
      121 gtatcaggtt caatgggtat catctcagcc taaagcaccc caaatgcctt tattatttaa
      181 ttatgtgatt attataacac acattttata ctagttcaag aaattgaact ggaaatacag
      241 ccttgcactc acaaagacag cagatctttc ttttgcaaaa aacaaatgac ctgtttgatg
      301 aattagctat tcaagctgaa tctagacata gctttttaaa aaaggaaaat cctacttact
      361 tagaatccaa ggatagatat ctattgttca ctcatttccc gaacagtttt ttctatattt
      421 tttgcatacg atattgccga aatgattgaa acgccgtcaa cattggtctt cataatgtct
      481 ttaatatgtt tcgtctgtat cccaccaatt gcaactaagg gcatttgtgg caatagtttt
      541 ctcatcaatt taacaccttc ataacctata gcaccaccag catcatcctt tgactgggta
      601 tcaaatacag gaccaacacc aacataatct acatattcaa cttttgattg ttgaaattct
      661 tcctcgtttt ttatagaaag accaattatt ttatctggca tcaattttct aatttcatca
      721 acaccaatat catcttgacc tacatgtacg ccatcagcgt caatttccat tgccaaatcg
      781 atatcatcat taacgataaa cggcacattg tattttttac aaagttcttt aatttggata
      841 gctagctcaa gtttttctaa gccttctaaa gcaccctcac ctttttctcg aaattgaaat
      901 aaggttatac cacctcttaa ggcttcctca acgactgtat atagattttt tccttggcaa
      961 gtagtcgttc cacaaataaa atatagtttt agtaattctt tatgaaacat cttacttcac
     1021 tcttttgaat tcctttacat cttcatctgt aatctcgtat aaggcattta taaattcaac
     1081 tttaaatgtc ccaggaagat gtccatttgg acgtttttct gccatttctc cagcgatatt
     1141 gtaaaccaac attgctgttt ctaatgattt caattcttga cctttttcta gtccgataaa
     1201 gcttgctacc actgccccta ataaacatcc agttccaata acttttggca tcatggcact
     1261 accattatga atcgttacca cttctccatt taccgcaata gcatccactt cacctgttac
     1321 tactattgga atattgaact tctcatttgc tgctagagca atctcgtcaa tattatctac
     1381 tcccgcacta tctactcctt tagatgccac atctattcct actaaagagg caatctctcc
     1441 agcattcccc ctaatcgcag ctagtttgta attgttgatt aaatcatctg ctactttttt
     1501 tctatattct cctgctccac aggctacagg atctaaaact actgggacat tatatttctc
     1561 tgcaattttc agagcagctt ggtataattt ccaattttca tctgtcaatg ttcctatgtt
     1621 tattaataaa ccaccagcat actttaacaa atcctctaaa tctgctggaa actcactcat
     1681 ggctggtgag gcgcccagtg ctactaatcc atttgctgtg aaatttttta ctacatcatt
     1741 ggttatacaa atgaccaatg gtgctttttc ttttaataat tttaaacttg tcatattgaa
     1801 atccttcctt ttcactttat acgatctact gatttcgatt tatttctatc tttcgttaag
     1861 aatttttttc atttacattg aatgatttat actcaatgaa aatcaaagag caaactagga
     1921 agctaaccgc agattgctca aaacactgct ttgaacctgt agataagact gacgaagtca
     1981 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac catacctacg
     2041 gcaaggtgaa actgacgtgg tttgaagaga ttttcgaaga gtcttacctc atcaaatttg
     2101 taaatatcat gagccttctc tagacatcgt aaccaatatc aaaaaaagct aattctaaag
     2161 cgactgcttg attccatcgt tgctgaagtt ctgtcaaatc ttctcgattt ttaccgacac
     2221 gattgagttc gtcaaccaga aattgaaccc actctgcaaa gaaaggacct ctgtggagat
     2281 tgatccattc cgaatgaata tagacttcag gtagagccaa atctttagaa ccccagtcta
     2341 aatagagacc ttctgcaatg accagcataa ccaaaagatg ggcatagtct gatgaagcca
     2401 ctgcccaata cattagatcc tgaaaggctt ttgttacagg gtgcaaagtc acttctagat
     2461 agtcattctc tgctactttt aactctttaa aagccttttg gaaataacca tcttcatctg
     2521 cttcaagaaa gcctagttgc ttggcaaaac gaagcttgga ttcaagttta tctgcgtgag
     2581 ctacgcaggc acccagcatg gataagaagg catcaaagaa gtgataatct tgaatcagat
     2641 agtcctttaa gaccttattc tcaattgtcc ccgcaaaaag ttccttaaca aaacgatgat
     2701 tgattgcagc ctgccaatcc ttctgactgc tttttaataa ttctccaaca gtcaaacctg
     2761 gctgaaatgc atagtcttgt gtttccatat ttacttctcc tctctttact tgttagtaat
     2821 taataaaaca ccaagaaata tcaagcaaaa tcgtaattcc acttgatcct tttaaaacac
     2881 atcgagagta tttgcagaga gctaactaaa caagcctatc cagtttatat aaacaaaaaa
     2941 ctccaattac aatcaagaat tagagttgac ttacaagatt agaccgttca tttcaccata
     3001 cgaaaaaact gttcacattt cccttcgcca gtcttaactg tatcaggttc aatgggtatt
     3061 atctcagcct aaagcacccc aaatgtctct attatttaat tactgaacca gtatagcaaa
     3121 aaatgaaagc cttagcaaga tatttaaccg aaaaatatct ttatatatag tagattgaaa
     3181 ctagaatagt acccctctac ttctaaaaca ttgttagaaa tcgatttaaa tatcctgatt
     3241 gatttgtcct gtttttattc cattttacta tggttttcga tagcgattta ttcttccaat
     3301 acacgaagaa aaacctccac attcagtgga ggcaatctgt tttatcaata caattttaag
     3361 tcacgagggt caactgggaa ggttgggttg tatggattgt gacggagctt aaagtgtttg
     3421 acatcttcaa tggtctgagc tccagacaat tgcataactg tcttcaattc cgcattcaag
     3481 tgttcaaaga cttgacgcac accgacacta ccaccgagag ccaagccata aataaccggg
     3541 cgaccaatag ctaccaagtc tgctcctgaa gccaaggctt taaagacgtg ttgaccacga
     3601 cgaacaccag agtcaaagac aatcggcaca cgtctatcaa ctgcttctgc cacttcttga
     3661 agcgagtcaa aggcagctgg tccaccgtcg atttgacgac caccgtggtt ggttacccag
     3721 ataccagaag ctcctgcagc aagcgaacgt tcaacgtcct cacggcattg tggtcccttg
     3781 acatacacag gaagaccaga gtattcagcg ataaattcta catcgcgtgg agacaagcgt
     3841 tgtttagctg atttgtaaac aaagtccatt gatttaccag caccttctgg caggtattct
     3901 tcaacaatcg gcatgccaac tgggaagaca aaaccattac gcttatccac ttcacgattc
     3961 ccccctacag tagcatctgc cgtcaagaca atcgctttat aaccttcatc cttcacacgg
     4021 tccatgatgt ggcggttgat accgtcatcc ttactaaagt aaaattgaaa ccaatgaggt
     4081 gtcccttgaa gggcttcaga aatctctgga aggtcaacag tagagtaaga actggttgta
     4141 taaagagaac caaactcatg cacaccacgc gcagtcgcca cttccccctg ttcatttgcc
     4201 aatttatgag ccgcaacagg tgccataatg attggcgaag atagtttttc acctgcaaat
     4261 tcaatctctg tacttggatt ttcaacatcg caaagtgtat gaggaacgat gagtttgtgg
     4321 ttaaaggcgc ggatattctc tcttaaagtg aaagtatctt ccgccccact agcgatatag
     4381 ccaaatgctg ctttaggaat aacttgttgc gccattggct ccaaatcata ggtattgatg
     4441 aagtctacat gaccttctgc attgcttgtt ttgtatgaca taaactgtcc tcctcgatta
     4501 agtaagcgtt tgctttttac tatacaacta taataactct tttttcaatc attttcaaga
     4561 gttttgctta aaaaccatct tattttttca tacaatatac tagccctact ttccttaaac
     4621 tctaaacctt agtctatatt aagtgaatcg ttgagatttt ttgctgccag gcatttgaac
     4681 tactttttta tttgctgatt ctatctacta atactataaa aaacaagtga ataagacacc
     4741 agaacatcct ctaataggac ctatggactt atatcagtcc catatatact gtttaagcaa
     4801 gatactatcc tagttgacgc taacttccta tcccacttat taatttttta tttatagtga
     4861 atctgaacaa atcaattggg gaatccaagt caatttctag tactttttga aaagtttcag
     4921 cgtactatcg taaaatgaaa taaaacatgc acaaattgat tagggaattt aaaccaattt
     4981 ctaacaatgt cttagaaatc aaagtgtact atttcaattt caatacacta cacttcattt
     5041 tctccttaaa ttcagtctct ttttgaataa aaaaatcctc tggtaagagc cagaggataa
     5101 gaatttattt cattgttggg aagagcaata catcacggat agttgttgta tcagtgagga
     5161 gcatgcagag acggtcgata ccgattccca aaccacctgt tggtggcata ccgtattcaa
     5221 gagcttcaat gtagtcatag tcgattcctg tcgcttcatc atcaccaagt tctttggctt
     5281 tagcttgggc ttcaaaacgg ctaagttggt cgattgggtc gttgagctca gtaaaggcat
     5341 taccgtactc cttagtcatg ataaagagct cgaaacggtc agtaaagcgt tggtcttcag
     5401 gatttttctt agcgagtgga gatacagcta ctggatgtcc atagacaaag gttggttgga
     5461 ttaaagtttc ttcaacaaac tcttcaaaga aggcattgat gatgtgacca acctcagtgt
     5521 agtgtttctc aactggaact ttcttctcag cagcgatagc tttagcttct tccaaagtca
     5581 tgtcttgcca gaaatcgaca ccagtaattt ctctgatagc atccaccata tgaacacgct
     5641 taaatggttc gttaatcttg atttcagtac cttgatagtt gactgggcca tcgcccttaa
     5701 ctgatttagc agcatgttgg ataatgcctt ccgtcaagtc catgatatct tggaagtctg
     5761 cataagcttg gtaaacttcg atagaagtga actcagggtt atgagtagca tccattcctt
     5821 cgttacggaa gatacgccca atttcataga cacgttccat accacccacg ataaggcgtt
     5881 ttaagtgaag ctcagtcgcg atacgaagca ccatgtcaat gttttgggca ttgtggtggg
     5941 tgataaatgg acgggcagca gcaccaccgg cttcgttgtg aagaacaggt gtttccactt
     6001 caaggaatcc tttttggtca aggtaacgac ggatttcaga gatgattttt gaacgagtga
     6061 caaaacgttc aaagctttca cggttagaaa tcaagtcaag gtaacgttta cggtaaattg
     6121 tttcaacgtc tgtcaaacca tggaatttct caggaagagg acgaagagcc ttagacaagt
     6181 gtgtgatgtg ggttgccttg atagagagtt ctcccatatc cgtacgcatc acttcacctt
     6241 cgacaccaag gaagtcacca aggtctgctt ttttgaagat ttcgtagttt tcttcaccga
     6301 cagcatcctt acgaacatag atctgaatct ggccttcgcg gtcttgaagg tgggcaaacc
     6361 ccactttacc tttaccacgt ttggttacca agcgtcctgc gatagtagct gtttcgtttt
     6421 tatcgtgtaa ttgttcttta tcgaggttgg catatttatc ttttaattct tgtgaatttg
     6481 cagtacgttc aaaacgtttt ccgaaaggat cgattccttg ttcgcggagc gcagccattt
     6541 tttcacggcg aacgatctgc tggtcattta gttcttccat atgttctgta gacatgggat
     6601 cctcctagtt ttactcaaaa ttgaatacga tgagtcattt tttgcgatac tccca
//
LOCUS       DAVMHG010000112         6653 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMHG010000112 DAVMHG010000000
VERSION     DAVMHG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.114"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            121..2100
                     /locus_tag="VYR08_001696"
     CDS             121..2100
                     /locus_tag="VYR08_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3467190.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2167..3663
                     /locus_tag="VYR08_001697"
     CDS             2167..3663
                     /locus_tag="VYR08_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467191.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3663..4589
                     /locus_tag="VYR08_001698"
     CDS             3663..4589
                     /locus_tag="VYR08_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467192.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4598..5665
                     /locus_tag="VYR08_001699"
     CDS             4598..5665
                     /locus_tag="VYR08_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467193.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5676..6602
                     /locus_tag="VYR08_001700"
     CDS             5676..6602
                     /locus_tag="VYR08_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467194.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         1980 a         1247 c         1384 g         2042 t
ORIGIN      
        1 aaaaaaaatg aaaaatttgt gccaacaagc ctaaatattc tgaaaatttg tttgattttt
       61 aatggataat gtgatataat ggttgtcaat ctataattca cataaacaga ggagtagaaa
      121 atgaaaaaaa atcgtgtatt tgctacagca ggtcttgttt tattagcagc aggtgtactt
      181 gcagcatgca gttcttcaaa atcatctgat tcatcagccc ctaaagctta tggctatgtt
      241 tatacagcag acccagaaac cttggactac ctgatttcaa gtaaaaatag tacaacagta
      301 gtgacttcaa atgggattga tggtttattc actaacgata attacggtaa tcttgctcct
      361 gcagttgcag aggattggga agtctctaag gatggtttga cctacactta taagattcgt
      421 aaaggggtta aatggtttac ctctgatgga gaagaatatg cagaggtgac ggctaaagat
      481 ttcgtgaacg gtttaaaaca cgcagcagat aaaaaatcag aagctatgta tttagctgaa
      541 aattcggtta aaggcttggc agattatcta tcaggaactt caacagattt ttcaacagtt
      601 ggtgtcaagg cggttgatga ttatacgtta caatacactt tgaaccagcc tgaaccgttc
      661 tggaactcta agttgaccta ttctattttc tggcctctga atgaagaatt cgaaacatca
      721 aaaggaagcg attttgctaa accaacagat ccgacatcct tgctttataa tggtccattc
      781 ttgttgaaag ggttgactgc aaaatcttct gtagagtttg taaaaaatga gcaatattgg
      841 gataaagaaa atgtccacct agatactatc aatctagctt actatgatgg atcagatcag
      901 gagtcgctag agcgtaactt cactagtgga gcttatagtt atgcccgtct ttaccctacc
      961 agctccaact attctaaggt tgcagaagaa tacaaggaca atatctatta cacacaatca
     1021 ggctctggga ttgctggtct gggtgtgaat attgatcgcc aaagttacaa ctatacttct
     1081 aaaactacag attcagagaa agtagctact aagaaggcat tgcttaacaa agatttccgt
     1141 caagccttga attttgctct tgatcgctca gcttactcag ctcaaatcaa tggtaaagat
     1201 ggagcagctt tagcagttcg taatttattt gtaaaaccag actttgtttc agctggtgag
     1261 aagacctttg gtgatttagt cgctgctcaa cttcctgctt atggtgatga gtggaaaggt
     1321 gtgaatttag ctgatgggca ggatggttta ttcaatgctg acaaggccaa ggcagagttt
     1381 gcgaaagcta agaaagcttt agaagcagac ggcgttcagt ttcctattca tctggacgtt
     1441 ccagtagacc aagcatcaaa aaactacata tctcgtattc agtcctttaa acaatctgta
     1501 gaaacagttc ttggtgttga aaatgtcgtt gttgatattc aacaaatgac aagtgatgaa
     1561 ttccttaata ttacttacta tgctgccaat gcttcatctg aggattggga tgtatcagga
     1621 ggagtttcat gggggccaga ctatcaagac ccatctactt acctggatat tttaaaaaca
     1681 actagcagtg aaactacaaa aacatattta ggatttgata atccaaatag cccttcagta
     1741 gttcaagttg gtttgaaaga atacgataaa ttagttgatg aagctgccaa agagacaagc
     1801 gacttgaatg tccgttatga aaaatatgca gcggctcaag catggttgac agatagttca
     1861 ctctttattc ctgctatggc ttcttctggt gcagcaccag tgctttcacg aattgttcca
     1921 tttactggag cttctgcgca aacaggctct aaggggtcag atgtttactt caaatatttg
     1981 aaattacaag ataaagcggt gactaaggaa gagtatgaaa aagctcgtga aaaatggttg
     2041 aaagaaaaag ctgaatcaaa tgagaaagct caaaaagaat tggcaagtca tgtgaagtaa
     2101 ataatttcta atagaacttt ctctccatga ggagaaggtt cttttgggat ttttaaagga
     2161 aataatatga aaaaatatat ttttatgcgt gttttgcggt cattggtttc gattttctta
     2221 gtaacgactt tgacctacac gattatctat accttggttc ctcgaaaatt gattttcaag
     2281 caggatccta actataataa aattgcgaca acggctgata aacgtgataa ctatgaaaat
     2341 actgtgtttg agcgtatggg ctacattgag tattacgata ctaaagagtt gcaagaaaag
     2401 gcaagtagca tggattcttc tgtaacagta gaagcaaatg cgaccaataa agctatttat
     2461 gaaaagtaca tcaatcaatt aggtcatggt tggactttgg gagaatttac tgaaagtggt
     2521 caattctatg ctactcgtga aattccaatt tttgaacgtg tttttcactt ctatgctaac
     2581 ttgattgaca ttgaccatac aaataaaatc caagaccctg aaaatccaga cttgaaacgc
     2641 taccttcgtt ttgaaaatga tccagctatc ggatggtcat tggtcggttc aggaactaaa
     2701 cataaatatc tcttgtactt taacagtcag ttcccatttg ttcatcaaaa ctttgtgaac
     2761 ttgaatttag gtgactctta cccaacctat gctaatacac cagttcttca ggttattact
     2821 caaggtcaag gacaaaccaa aactgcccaa gttcagttcc caacaggtaa gaaaacgtct
     2881 tctgtaaata tttactcaag aacctacaag tcacctagtc aggctgactc tcgtgaagta
     2941 gctagctatg ggaaagatga tccttataca gcgactgaaa gtaattacca atatccatct
     3001 atgattgtca gctctgctat tactggtttg attggtttgg ttcttgccta tgctcttgcc
     3061 gtgccacttg gttcagccat ggctcgtttc aagaacactt ggattgatag cctctcaaca
     3121 ggggctttga ccttcttgct tgctcttcca acgattgcct tggtttacat cgttcgattg
     3181 attggatcat ctattgccct tccagattca ttccctatct tgggagctgg agattggcgt
     3241 tcttacgttt taccagcagt catccttggt ttgttgggtg ctcctggtac agccatttgg
     3301 attcgtcgtt acatgattga cttgcaatct caagactttg ttcgtttcgc tcgtgcaaaa
     3361 ggtttgtctg aaaaagaaat ttcaaacaaa cacatcttta aaaatgccat ggttccgctg
     3421 gtttcaggaa ttcctgctgc cattattggg gttatcggtg gtgcaaccct tactgaaaca
     3481 gtcttcgcct tcccaggtat gggtaaaatg ttgattgact ctgtaaaagc atctaataac
     3541 tctatggtcg ttggtcttgt cttcatcttt acatgtattt ctatcttctc acgtcttttg
     3601 ggagatattt ggatgactat tattgaccca cgtattaaat tgactgagaa aggaggcaaa
     3661 taatgtctac aatcgataaa gaaaaatttc agtttgtaaa acgtgacgat tttgcctctg
     3721 aaactattga tgcgccagca tattcttact ggaaatcagt gtttaaacaa tttatgaaga
     3781 aaaaatcaac tgtagtcatg ttgggaatct tggtagccat cattttgata agtttcatct
     3841 acccaatgtt ttctaagttt gatttcaatg atgtcagcaa ggtaaacgac tttagtgttc
     3901 gttatatcaa gccaaatgcg gagcattggt tcggtactga cagtaacggt aaatcgctct
     3961 ttgacggtgt ctggttcgga gctcgtaact ccatcctcat ttctgtgatt gcgacagtga
     4021 ttaacttggt tatcggtgtt tttgtcggtg gtatttgggg tatttcaaaa tcagttgacc
     4081 gtgtcatgat ggaagtttac aacgtcatct caaacatccc acctcttttg attgttattg
     4141 tcttgactta ctcaatcgga gctggattct ggaatctgat ttttgccatg agcgtaacaa
     4201 catggattgg tattgccttc atgatccgtg tgcaaatctt gcgctatcgt gacttggaat
     4261 acaacttggc gtcacgtact ttgggaacac caaccttgaa gattgttgcc aaaaatatca
     4321 tgcctcaatt ggtatctgtt attgtgacaa ccatgactca aatgcttcca agctttatct
     4381 catacgaagc cttcttgtct ttcttcggtc ttggattacc gattacagtg ccaagtttgg
     4441 gtcgtttgat ttcggattat tcacaaaacg taacaaccaa tgcttacttg ttctggattc
     4501 cattgacaac ccttgtcttg gtatccttgt cccttttcgt agttggtcaa aacttagcgg
     4561 atgctagtga tccacgtaca catagatagg agtagaaatg acaaaagaaa aaaatgtaat
     4621 tttgactgct cgcgatattg tcgtggaatt tgacgttcgt gacaaagtat tgacagccat
     4681 tcgcggcgtt tcccttgaac tagtcgaagg agaagtatta gccttggtag gtgagtcagg
     4741 atcaggtaaa tctgttttga caaagacctt cacaggtatg ctcgaagaaa atggtcgtat
     4801 tgcccaaggt agtattgact accgtggtca ggacttgaca gctttatctt ctcacaagga
     4861 ttgggaacaa attcgtggtg ctaagattgc gactatcttc caggacccaa tgactagttt
     4921 ggaccccatt aaaacaattg gtagtcagat tacagaagtt attgtaaaac accaaggaaa
     4981 aacagctaaa gaagcgaaag aattggccat tgactacatg aataaggttg gcattccaga
     5041 cgcagataga cgttttaatg aatacccatt ccaatattct ggaggaatgc gtcaacgtat
     5101 cgttattgcg attgcccttg cctgccgacc tgatgtcttg atctgtgatg agccaacaac
     5161 tgccttggat gtaactattc aagctcagat tattgatttg ctaaaatctt tacaaaacga
     5221 gtatcatttc acaacaatct ttattaccca cgaccttggt gtggtggcaa gtattgcgga
     5281 taaggtagcg gttatgtatg caggagaaat cgttgagtat ggaacggttg aggaagtctt
     5341 ctatgaccct cgccatccat atacatggag tctcttgtct agcttgcctc agcttgctga
     5401 tgataaaggg gatctttact caatcccagg aacacctccg tcactttata ctgacctgaa
     5461 aggggatgct tttgccttgc gttctgacta cgcaatgcag attgacttcg aacaaaaagc
     5521 tcctcaattc tcagtatcag agacacattg ggctaaaact tggcttcttc atgaggatgc
     5581 tccaaaagta gaaaaaccag ctgtgattgc aaatctccat gataagatcc gtgaaaaaat
     5641 gggatttgcc catctggctg actaggagga aggaaatgtc tgaaaaatta gtagaaatca
     5701 aagatttaga aatttccttc ggtgaaggaa gtaagaagtt tgtcgcggtt aaaaatgcta
     5761 acttctttat caacaaggga gaaactttct cgcttgtagg tgagtccggt agtgggaaaa
     5821 caactattgg tcgtgctatc atcggtctaa atgatacaag taatggagat atcatttttg
     5881 atggtcaaaa gattaatggt aagaaatcgc gtgaacaagc tgcggaattg attcgtcgaa
     5941 tccagatgat tttccaagac cctgccgcaa gtttgaatga acgtgcgact gttgattata
     6001 ttatttctga aggtctttac aatcaccgtt tatttaagga tgaagaagaa cgtaaagaga
     6061 aagttcaaag tattatccgt gaagtaggtc ttcttgctga gcacttgact cgttaccctc
     6121 atgaattctc aggcggtcaa cgtcaacgta tcggtattgc ccgtgccttg gtcatgcaac
     6181 cagactttgt tattgcagat gagccaattt cagccttgga cgtttctgta cgtgcccaag
     6241 tcttgaactt gctcaaaaaa ttccaaaaag agctcggctt gacctatctc ttcatcgccc
     6301 atgacttgtc ggttgttcgc tttatttcag atcgtatcgc agttatttac aagggtgtta
     6361 ttgtagaggt tgcagaaaca gaagaattgt ttaacaatcc aattcaccca tatactcaag
     6421 ccttgctttc agcggtacca atcccagatc caatcttgga acgtaagaag gtcttgaagg
     6481 tttacgaccc aagtcaacac gactatgaga ctgataagcc atctatggta gaaatccgtc
     6541 caggtcacta tgtttgggcg aaccaagccg aattggcacg ttatcaaaaa ggactaaact
     6601 aataatggtt ttataatttc catgtcaatt tttatgggaa ttataaaact ttt
//
LOCUS       DAVMHG010000113         6611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMHG010000113 DAVMHG010000000
VERSION     DAVMHG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.115"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            170..370
                     /locus_tag="VYR08_001701"
     CDS             170..370
                     /locus_tag="VYR08_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467195.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            395..811
                     /locus_tag="VYR08_001702"
     CDS             395..811
                     /locus_tag="VYR08_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467196.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1042..1749
                     /gene="ccdA2"
                     /locus_tag="VYR08_001703"
     CDS             1042..1749
                     /gene="ccdA2"
                     /locus_tag="VYR08_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV3467197.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1760..2317
                     /gene="sdbB"
                     /locus_tag="VYR08_001704"
     CDS             1760..2317
                     /gene="sdbB"
                     /locus_tag="VYR08_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV3467198.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2692..4083
                     /locus_tag="VYR08_001705"
     CDS             2692..4083
                     /locus_tag="VYR08_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV3467199.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4226..5143
                     /gene="adcAII"
                     /locus_tag="VYR08_001706"
     CDS             4226..5143
                     /gene="adcAII"
                     /locus_tag="VYR08_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV3467200.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5151..>6611
                     /gene="phtD"
                     /locus_tag="VYR08_001707"
     CDS             5151..>6611
                     /gene="phtD"
                     /locus_tag="VYR08_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV3467201.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     NQAGQKAENLTPDEVSKREGISAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDV"
BASE COUNT         2030 a         1225 c         1397 g         1959 t
ORIGIN      
        1 ttaaaacgaa ataagaatag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagt ccatatgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc
      121 tgcaagtcct gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagtaag
      181 cgcttcggct agctcaacga gcccacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagttag accaacactt gaaagctttg gcaacagtat ctgatccgaa
      301 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa
      421 agatgtgaaa gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca
      481 aggaacggaa gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg
      541 agtgattgtg gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg
      601 taagaaagag tcagaaatcc agcaattaag cacggaatta atcaaggttc taggacagct
      661 agatgcagaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac
      721 ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga
      781 gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacttaaa
      841 agacattttc gttcacaaaa gcagcgacga gtaatccttg tcgttgcttt ttggctattt
      901 tgggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac
      961 gattttgcgt agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt
     1021 attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca
     1081 ggtgttttat cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt
     1141 ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg
     1201 tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta
     1261 ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg atatggcatg
     1321 ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta
     1381 gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt
     1441 ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct
     1501 gttttagcac ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat ttatactctc
     1561 atttacactc tgggcatggc ccttcctttc ttggtattgg cactagcttc aggtctagtc
     1621 atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc
     1681 ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt
     1741 tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt
     1801 agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc
     1861 aaaacaacaa acgactgtac aacaaattgc tgttggaaaa gatgctccag actttacatt
     1921 gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt
     1981 gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact
     2041 agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg
     2101 tgaaaaaact gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc
     2161 agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac
     2221 agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa
     2281 tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag
     2341 atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa cctagctttt
     2401 agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt
     2461 tctttttctt gatatataag ggttcaaaag cgaaaagact cagaaaaaag tgcacgacaa
     2521 atagccctaa aacagagtcg ttttagagta acttccagtt ttgggagaga gatggaagtt
     2581 actttgagaa gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata
     2641 tctcctatat ttatggtaaa atagaatcat cagtttattt tggagtcaaa gatgaatata
     2701 tttagaacaa agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg
     2761 gatttgattt tgctgggtat cggagccatg gtagggacag gcgtctttac aatcacaggt
     2821 actgcagctg caacacttgc tggcccagcc ctagtgattt caatcgttat ttctgccttg
     2881 tgtgtgggat tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga
     2941 ggtgcctata gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg
     3001 ttaaccatga tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat
     3061 tttaaaggct tgctctctca atatgggata gcccttcctc aggctttaaa tggtaccttt
     3121 aatcctcaag cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact
     3181 tcgcttgttt tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg
     3241 aaattttccg ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat
     3301 tggagcaatt ttgctcctta cggctttgga caaatctatg gagctagtac tggtattatg
     3361 gctggtgcgt ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta
     3421 gatgaagtca agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc
     3481 gtaaccattc tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat
     3541 ttaaatgtcg atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc
     3601 aactatgtgt cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat
     3661 gccctatcgc gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag
     3721 gaactgacga agactagcaa ggtaccaaag aatgctacta tcttgacagg tctagcttca
     3781 gcagtagcag caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc
     3841 ttagcctact tgatcatgct ggcttatggt ctgattcgct tacggaaaga aaaaggaatg
     3901 cccaaagctg gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc
     3961 atttgtttat ccttcatgtt acagtataat atgaacacct ggattgcttt cctagttgcg
     4021 ttgttagtag gaagtattat ttacttcact tatggctata agcattctac catagaagaa
     4081 taaagtgaaa gtctattgag ttgttgacac tcagcagatt tttatatgtg aaagaaattt
     4141 caaatttttt ctaaaaatat cttgacagat taaattttca ggagtagaat atttactagt
     4201 taattaaatg gttaaggagt tgttcatgaa gaaacaaaat ttatttttag tcctgttaag
     4261 tgtctttctt ttgtgcttgg gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat
     4321 gaaaattgtg accagttttt atcctatcta cgctatggtt aaggaagtat ctggtgactt
     4381 gaatgatgtt cggatgattc agtcaagtag tgggattcac tcctttgaac cttcggcaaa
     4441 tgacatcgcg gccatctatg atgcagatgt ctttgtttac cattctcata cgctagaatc
     4501 ttgggcagga agtttagatc ccaatctaaa aaaatctaaa gtgaaggttt tagaggcttc
     4561 agagggaatg actttagacc gcgtcccagg actagaggat gtggaagcag gggatggagt
     4621 tgatgaaaaa acgctctatg accctcacac ttggctagat cctgaaaaag ctggagaaga
     4681 agcccaaatt atcgctgata aactttcaga ggtggatagt gagcataaag agacttatca
     4741 aaaaaatgcg caagccttta tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa
     4801 atttgaaaaa gcgactcaga aaacatttgt aacacaacat acagcctttt cttatctagc
     4861 gaagagattt gggcttaatc aacttggtat tgcaggtatc tctcctgaac aagaaccaag
     4921 tccacgacaa ctaacagaaa ttcaggaatt tgttaagacc tataaggtta aaacgatttt
     4981 tacagaaagt aacgcttctt caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg
     5041 tcttaaaact ctgaatcctt tagagtcaga cccacaaaat gacaagacct atttagaaaa
     5101 tcttgaagaa aatatgagta ttctagcaga agaattaaag tgaggaaaga atgaaaatca
     5161 ataaaaaata tctagcaggt tcagtggcag tccttgccct aagtgtttgt tcctatgaac
     5221 ttggtcgtca ccaagctggt caggttaaga aagagtctaa tcgagtttct tatatagatg
     5281 gtaatcaggc tggtcaaaag gcagaaaatt tgacaccaga tgaagtcagt aagagggagg
     5341 ggatcagcgc cgaacaaatc gtcatcaaga ttacggatca aggttatgtg acctctcatg
     5401 gagaccatta tcattactat aatggcaagg tcccttatga tgccatcatc agtgaagagc
     5461 tcctcatgaa agatccgaat tatcagttga aggattcaga cattgtcaat gaaatcaagg
     5521 gtggttatgt cattaaggta aacggtaaat actatgttta ccttaaggat gcggcccatg
     5581 cggacaatat tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc agtcataatc
     5641 acaactcaag agcagataat gctgttgctg cagccagagc ccaaggacgt tatacaacgg
     5701 atgatgggta tatcttcaat gcatctgata tcattgaaga tacgggcgat gcctatatcg
     5761 ttcctcacgg cgaccattac cattacattc ctaagaatga gttatcagct agcgagttag
     5821 ctgctgcaga agcctattgg aatgggaagc agggatctcg tccttcttca agttctagtt
     5881 ataatgcaaa tacagctcaa ccaagattgt cagagaacca caatctgact gtcactccaa
     5941 cttatcatca aaatcaaggg gaaaacattt caagcctttt acgtgaattg tatgctaaac
     6001 ccttatcaga acgccatgtg gaatctgatg gccttatttt cgacccagcg caaatcacaa
     6061 gtcgaaccgc cagaggtgta gctgtccctc atggtaacca ttaccacttt atcccttatg
     6121 aacaaatgtc tgaattggaa gaacgaattg ctcgtattat tccccttcgt tatcgttcaa
     6181 accattgggt accagattca agaccagaac aaccaagtcc acaatcgact ccagaaccta
     6241 gtccaagtcc gcaacctgca ccaaatcctc aaccagctcc aagcaatcca attgatgaga
     6301 aattggtcaa agaagctgtt cgaaaagtag gcgatggtta tgtctttgag aagaatggag
     6361 tttctcgtta tatcccagcc aagaatcttt cagcagaaac agcagcaggc attgatagca
     6421 aactggccaa gcaggaaagt ttatctcata agctaggagc taagaaaact gacctcccat
     6481 ctagtgatcg agaattttac aataaggctt atgacttact agcaagaatt caccaagatt
     6541 tacttgataa taaaggtcga caagttgatt ttgaggcttt ggataacctg ttggaacgac
     6601 tcaaggatgt c
//
LOCUS       DAVMHG010000114         6565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMHG010000114 DAVMHG010000000
VERSION     DAVMHG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.116"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(261..341)
                     /locus_tag="VYR08_001708"
                     /pseudo
     CDS             complement(261..341)
                     /locus_tag="VYR08_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(332..1621)
                     /locus_tag="VYR08_001709"
     CDS             complement(332..1621)
                     /locus_tag="VYR08_001709"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV3467202.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1701..2948)
                     /locus_tag="VYR08_001710"
     CDS             complement(1701..2948)
                     /locus_tag="VYR08_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV3467203.1"
                     /translation="MKLLFRNPAYRILTLSCFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGDLGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(3141..3992)
                     /gene="rgg"
                     /locus_tag="VYR08_001711"
     CDS             complement(3141..3992)
                     /gene="rgg"
                     /locus_tag="VYR08_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV3467204.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSAFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            4116..4457
                     /locus_tag="VYR08_001712"
     CDS             4116..4457
                     /locus_tag="VYR08_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV3467205.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4515..4595
                     /locus_tag="VYR08_001713"
                     /pseudo
     CDS             4515..4595
                     /locus_tag="VYR08_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4711..6414)
                     /gene="ilvD"
                     /locus_tag="VYR08_001714"
     CDS             complement(4711..6414)
                     /gene="ilvD"
                     /locus_tag="VYR08_001714"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV3467206.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT         2005 a         1449 c         1298 g         1813 t
ORIGIN      
        1 tttctgcttc ctatttctta acttgaattc attgtaacat atctttttct ttttgacaat
       61 tatagttgtc aaaaagttta tgatttttgt cattttgcaa aagaaaaagg tcaggagtag
      121 gttcctgacc actttatcta tcattaatac tcttctaaaa tctcttcaaa ccacgtcagc
      181 ttcaccttac cgtaggtatg gttactgact tcgtcagttt catctacaac ctcaaaacca
      241 tgttttgagc tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagcaacct
      301 gcggctagct tcctagtttg ctctttgatt tttattgagt ataaaatcct agtttttcaa
      361 agatttctga gaagttttgg ctgattgtct caagtgacac ttgcacttct tctcgagttt
      421 ggttgttctt gaccgtcact tgtccgcttt cgacttcgct ctctcctagg gtgatgaggg
      481 tcttagccac aaagacatcg gctgacttga actgagcttt tagtttacgg ttgaggtaat
      541 cacgctctgc tttgaaacct tgttggcgaa gagcctgtac caattccaag gccttgatat
      601 ttgccccttc gcccaagact gcaatataga catctagggc gttttcgata gggagggtca
      661 cgccttgttt ttcaaggatg agaagcaggc gctctacacc aagtccaaaa ccaaatccag
      721 cagtttcagg gcctccaaag taagcaacca aaccatcgta gcgaccaccc gcacagactg
      781 tcaagtcatt gccctcaatc tctgtgataa actcgaaaat ggtgtggttg tagtagtcca
      841 gaccacgcac catattggta tcgatgatgt aatctactcc aagattttcc aacatctgac
      901 gcacagcatc aaaatgagtt tggctttctt catcaagaaa gtccaagata gacggcgcat
      961 tctctactgc caccttgtct tctttttcct tagagtccaa gacacgaaga ggattttcct
     1021 ccaagcgacg ttggctatcc ttagacaagg tctccttgag cggtgtcaaa tagtcaatca
     1081 aggcttggcg gtaggctgca cggctctcag gatttccaag agtgttgagg tgcaatttga
     1141 caccttgaat accgatttcc ttcaaaaaat gggctgccat agcgattgtt tccacatcgg
     1201 tagctggatt gctagagcca aaacactcaa caccaatctg gtggaattgg cgcaagcgcc
     1261 ctgcctgtgg acgctcataa cggaacatag gtcccatata gtagaacttg cttggctttt
     1321 gcacttctgg ggcgaagagt ttattttcca cataggaacg gacaacgggt gcagttcctt
     1381 ctggacggag ggtaatatga cggtcaccct tgtcataaaa atcgtacatt tccttggtta
     1441 cgatatccgt tgtatctccg acagagcgac tgataacctc gtaatgctca aaaataggcg
     1501 tgcgcacttc cgcgtagttg tagcgtttga aaatttcacg ggcaaagccc tcaacgtact
     1561 gccacttggc agattcagca ggtaaaatat cctgcgttcc ttttggtttt tgtaatttca
     1621 tagggaatcc tctttaaact taatagtctt attttaccac aaataaaggg attaaaacag
     1681 tgagaaaaaa aaattaggat ttagatatca tttttgagat taagaattgt taaaaaaata
     1741 gctagcaagg aaagaccaac aaatagcatc caagtcaact gtatattcca tacggctact
     1801 agtgaaaaac aagctgttcc cacaggtatg gataaggtaa acaatagacc taaaaaatta
     1861 ctagtacgag ctagaacctc tggagctaga tttttcatga gcatggcact aatctttggt
     1921 tgaactttac cagacacata cagagtaaag aagagaaata gcaaaccaag cacgacttga
     1981 ttgaataaat tagccaaacc aactagacta agccctacgg tcgcccacat catcaatcta
     2041 ggcaaggact gcttcccaaa ataatcattg cccgtaaggc tactgatgat gactgctact
     2101 aaaacacaga attgattgat aaatagtgcc tctgtataag aaaaattcaa gagagaatgg
     2161 ctcaaaaaga agatattata aattccaccc aaatcgccac ccaaggaatt aataagcaag
     2221 acagcaaaga gcataaaacc aaagtttttc tgcccacttt taagaaaaac gagacgtaaa
     2281 tttcggtaaa ttgttaggaa ctggtctttg atagaaagct tctcattttt taagttttca
     2341 ccatcagcag atgacattga caggctcaat ttgctttttc ctaaaaagag gatagtggct
     2401 gatactagga aaaagcaggc attgattccc gcaacgagag aaaaattgtt gaccgataga
     2461 gctaagagcc agactccgaa agcttgacca ccaatagctg aaatataggt gatgaactgt
     2521 gaaaaagaat aagcctccat caaatcatct tcagctactt tttccttaat aagaggcata
     2581 cgcaggccac ctgcaaaatc actgatgata tcactaatga cattgatcaa acacaggcta
     2641 gaaaaggcaa agagactagc ttgctgaaca actagggctg ctagaaaaaa tagaaccgcc
     2701 tgaaacaaac cgctatagac catccatttg accttgtccc tcgtgtaatc tgcccgaatc
     2761 cctacaaaaa ctgtaaagag ggtcggaaga atcatgacaa tattcgccat agcaacagca
     2821 aaagatgctt gtgacaaggt cgatgcatag acgataaaga ccaggttgaa aatcgaagca
     2881 ccaaaagcat tgaagaagca tgataaagtc aaaattctat aagctggatt tctaaacaat
     2941 agtttcatag ataatctccc ttcttgttcg aacctctata tgaagtatga ctatagtata
     3001 gcacttagaa atttgagagg gagaactctt gaatatatgc aacaaataaa atgagcaaaa
     3061 agaaacgata gaacttttgt aatgacctga gagagggcaa tagttctatc gcttcaaatt
     3121 ttttcttggt ttgcagatat tcaacattcg gtcgtatttc tatagtattc ggcagattta
     3181 ttacagccaa gcatctcaaa aatacggaca gcatcctcca tctttttctg gccttccttg
     3241 actctacctt gcttgctatc aaggagacct tctgcccaca gataaacaat tcggaaatag
     3301 gtctcatttt ccttgtagaa atgctcttcg ataacacgtt taaaataata ggcattggta
     3361 aattcttcac actcaatact agctaaaaag ccattcaata gtatagtatg aaaaaagttt
     3421 cgattaccag acatttccat tagaaaatca gatttacgta ccatttctcg tacatatcta
     3481 gtaaaaagag aaacagataa aaaggcagaa ctgactgaaa ataaattgag ttcatagatt
     3541 ccccagatct cggtagaaaa caaataatca tgaaggactt ttccttcctc tgctgttaac
     3601 tctacccttt catccatgct cttcatataa gacttgatca taatggcatt tagaatatgt
     3661 ttctgtttgt tgtgagaatg ggcatgcttt tgatactccc tgcgatataa gtcctcaaga
     3721 ggtgctatat tctttggttc caagacatct gtaatttctt ttctcaactc agaatctgta
     3781 tcatactgga aacctctcgc cagaaagagg atctcctcca cactggcaga tatattttcc
     3841 agagcaaata gaaacttttc caccgaaagc tcactctgac ctgtttcaaa acgggacaac
     3901 atagacggcg aaaattgtcc tccggttgct tgtctcagtg agatatttct tgattctcgt
     3961 aattgtctaa agacttttcc aatctgctcc atagacttcc ccttgattcc gtattttctt
     4021 cattttatca tatttttcag aaaattcatc aaaaacttgc caaattgtca gaattatgag
     4081 aaaatagagg atatttatca cgtggaggga ctgctatgag agacgatatc aaaatcaatg
     4141 accgtgcttt ggccttgcaa gaccaaatta tcgaaaaact agagaaagtt tttgatacag
     4201 atgtggaatt ggatgtttac aatctaggtc tgatttatga aatcaatctg gatgaaacgg
     4261 ggctctgcaa gattgtcatg accttcaccg atactgcctg tgattgcgcc gaaagcctgc
     4321 ctattgaaat cgtggcaggt ctgaaacaaa tcgagggtat caaagatatc aaggttgaag
     4381 ttacctggtc gcctgcctgg aaaatcacac gaatcagtcg ctatggccgt attgcccttg
     4441 gactaccacc tcgttaagca ggccaatcac ttttaaagat gaaaaaagca agccgaaaat
     4501 ggcttgcttt ttgatactca atgaaaatca aagggcaaac tagaaaacta gccgcaggtt
     4561 gctcaaaaca ctgttttgaa gttatggata gaactgacga agtcagctca aaacactgtt
     4621 ttgaggttgt agatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga
     4681 cgtgatttga agagattttc gagtatgagt ttatttttta cctgacttgt ccatattcca
     4741 gaagtctgtc acggctccgc gtgaagcaga tgatacgatg tgggcatatt taccgaggac
     4801 accacggctg taaagtggtg gcaaggttgt ttctgccttg cgtttttcaa gttcttcttc
     4861 ggatacggcc atggaaattt ctttggtatc ttggtcaacc gtaacgatat cgccggtacg
     4921 gagataggca attggtccac catcctgagc ttcaggagcg atatgtccaa caaccagacc
     4981 ataagtacca ccagagaaac gtccgtccgt caagagggcc accttatctc cctgaccttt
     5041 accaacaatc attgatgaaa gtgacagcat ctcaggcata ccaggaccac ctttaggtcc
     5101 aacaaaacga acaacgacta catcgccatc aacgatttca tctgtcagaa cggcctgaat
     5161 cgcatcttct tctgagtcaa agaccttagc tggcccaacg tgacgacgca ctttaacacc
     5221 tgataccttg gcaactgcac cgtcaggagc aaggttcccg ttcaagatga taagcggacc
     5281 atccgcacgt tttggatttt caagtggcat gataactttt tggcctggag tcaagtctgc
     5341 aaagtcagcc aagttttcag ctacagtctt accagtacat gtgatgcgat ctccgtgaag
     5401 gaaaccattt gccaacaagt acttcataac cgcagggaca ccaccgactt cgtagaggtc
     5461 ttggaagaca tactgaccag atggtttcaa gtcggccaag tgaggcacac gttcttgaat
     5521 cgtattgaag tcctcaagtg acaagtcaac atttgcggca tgggcaatgg cgagcaagtg
     5581 aagagtggcg tttgtagaac caccgagagc catcgttaca gtgatagcat cttcaaaggc
     5641 ttcacgagtc aagatatctg atggtttgag accaagttcc aacatcttaa caacagcacg
     5701 tcctgctgct tcgatatctt ctttcttatc agctgattca gctgggtgag aggatgaccc
     5761 tggcaaactc atccctagaa cttcgatagc agttgccatg gtattagcag tatacatacc
     5821 accacaacca ccagggccag ggcaggcatt acattcaaga cgtttcacgt cctcagctgt
     5881 catgtcaccg tggttccatt ttccgatacc ttcaaagaca gaaaccaagt cgatatcttt
     5941 accatcaaga tttcccggtg caatagttcc accataggcg aaaatagctg ggatatccat
     6001 attagcaata gcaatcatag atccaggcat gttcttgtca cagccaccga tagcgacgaa
     6061 ggcatccacg ttgtgaccac tcatagccgc ctcgatggag tccgcgatga tgtcacgaga
     6121 tgttagagag aaacgcatac caggcgttcc catagcgatc ccgtccgcta cggtaatggt
     6181 tccaaactgt acaggccaag cgcctgcaga tttgacacct tctttagcca gtttcccgaa
     6241 atcatgcaag tgaatgttac atggtgtatt ttccgcccaa gtcgaaatca ctcccacaat
     6301 cgatgtttca aagtccttat ctgtcatacc agtcgcacga agcatagcac ggttaggtga
     6361 tttaaccatg ctgtcataga tgctactgcg gtgacgttta tctaattcag tcatcttatc
     6421 cctcccattt cagtttttac tattatagca caatttaaag gcaatgaaca gaataaaatt
     6481 cttgaatttt cagaaaattc tatacgcatg tgaaatattt aaaattaaaa acaacaaagc
     6541 ggattagtgc actttctgat gacca
//
LOCUS       DAVMHG010000115         6451 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMHG010000115 DAVMHG010000000
VERSION     DAVMHG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.117"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            113..430
                     /locus_tag="VYR08_001715"
     CDS             113..430
                     /locus_tag="VYR08_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV3467207.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(475..1287)
                     /gene="folK"
                     /locus_tag="VYR08_001716"
     CDS             complement(475..1287)
                     /gene="folK"
                     /locus_tag="VYR08_001716"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV3467208.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLET
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(1330..1884)
                     /gene="folE"
                     /locus_tag="VYR08_001717"
     CDS             complement(1330..1884)
                     /gene="folE"
                     /locus_tag="VYR08_001717"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV3467209.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1865..3187)
                     /locus_tag="VYR08_001718"
     CDS             complement(1865..3187)
                     /locus_tag="VYR08_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV3467210.1"
                     /translation="MKEIENNQWIANYWTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYFIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(3189..4163)
                     /gene="folP"
                     /locus_tag="VYR08_001719"
     CDS             complement(3189..4163)
                     /gene="folP"
                     /locus_tag="VYR08_001719"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV3467211.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(4258..4965)
                     /locus_tag="VYR08_001720"
     CDS             complement(4258..4965)
                     /locus_tag="VYR08_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3467212.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLMVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLAFVVIMSRTLGISV"
     gene            complement(5018..6436)
                     /locus_tag="VYR08_001721"
     CDS             complement(5018..6436)
                     /locus_tag="VYR08_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV3467213.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
BASE COUNT         1918 a         1554 c         1211 g         1768 t
ORIGIN      
        1 ggaatttttt ccctatttct ccctgtaact aaaatccttt tcaaaaaatc accttttctt
       61 gatttttaat cctatttgct gtatgataag ggaaaagaaa ggggacagag atatggcttt
      121 taccaatacc cacatgcgat ctgctagttt tggcattgtt accagcttgc ctgatgacat
      181 cattgactct ttttggtata tcatcgacca tttcttaaaa aatgtctttg aattggaaga
      241 agaactcgag tttcaattgc ttaataacca aggaaagatt accttccact tttcaagtca
      301 acacctccct acagccattg attttgactt taaccatcct ttcgaccctc gttatccccc
      361 aagagtactg gttttagaca tggacggtag agaaactatc ctcctcccag aagaaaatga
      421 cctattttaa aaactctagc cttcagttgc aagtaactga aaactagagt ttttctattt
      481 tttcaaagca tcatacaagt tgcggatcgg ttgttttaat atcggatgga taaaatgagg
      541 cgcaatttcc tgtaaggact caaggacaaa aaggcgttcc gctatgtaag gatgaggcaa
      601 tatgaggtcg tctgtataaa ggatctggtc ctccacaaag agcaagtcca aatcaatcaa
      661 acgaggtccc caatgcactt ctctcacccg tcccagctct gactcaatgg ctaacaaggt
      721 ttctaacaag tcttgtgctg gtagccaggt ttccacctca accacttgat tggcaaagct
      781 atcctgctcc actccacccc aaggctccgt ctctaagaca ctggactctt tgagaatatg
      841 gatgccacga gctcgcagtt tgtcaatggc ttgcttcaag tttacttgtt tatctcccat
      901 attgcttcct agggcgataa aggctcgttg cttgcggcga tgaatggtta ccgagcaagt
      961 atctagtgac aaatgcaccg gtgcccaagg ttttttcagt tccaacttca tttcttggac
     1021 aagaggataa gactcaaagg tacgttccac cagtttatag gctaccgttt caatcaaatc
     1081 ttcactcgtt tcctgaaacc aagtcgtcca ctgctgacac aattctccgt aatggacaga
     1141 ggctgttaaa tccaagtctg tagctgcctt ggtcatatca taggatagga tggctgaaac
     1201 gacaaatttc tgccccaatt ctttctcact aggaaaaaga ccatgatagg caaacatttc
     1261 caaatcctta atctgcagtt gatccataat gattcctttc tagaaaaatc cgcttgaagc
     1321 ggattctttt tatagcccca ttaaacgata agcttggtcg cggaggtcct tatctgtttc
     1381 aaatagacca cgagctactg tcgtcaaggt tgcagtgcct ggttttctaa caccacgcat
     1441 actcatacac atatgttccg cctcaatgac aacaaaggct cctttagcac ctagatagtc
     1501 catcaaggca tcggccactt cgatattcaa acgttcttga atttgtggtt ttttcgaata
     1561 aacttcaacc gtacgggcta gcttagacaa gcctgccaca cgaccatctg gaatgtaggc
     1621 aatgtgcgct ctaccataaa atggcaagaa gtggtgttca cacatggtat ggaaaaagat
     1681 atccttttct accaccatat tatcgtcaat aatttcaaag gattttgaca aatgttcctc
     1741 tgctgtttga ccaagacctg aaaaaatttc ttgatacata cgggctacac gagcaggtgt
     1801 ttcctgcaag ccctcgcgat tagcgtcctc tcctacagcc tcgataatca tttttacagc
     1861 tgcttcaatc ttttgtgtat ccattctcgt tcttcctctc catcagatag gccctcactt
     1921 ggctcaagaa atacaaggaa cctgtgacaa tcctaactgt ttgtttctct tcttttttat
     1981 ctgtcaaatt ctgctctaga aaatcatgcc aatcttggta gctgagattt ctagacttag
     2041 ctgcctcttt cagcacgctt tcatccgtcg cccgactatc cgcaaaatgt gttagagtaa
     2101 gctcggtatc tggcatggct cccagcaagt ccaacatatc ctccaaggcc ttggttttga
     2161 tacaagtgaa gaggatttcc ttatgataat ccgcaaaacg ttcttgcaag gttaccaaca
     2221 aggccttgat agcatgggga ttgtgggctc catccaaaat catcaaggga tctcttgaca
     2281 cgatttccaa acgccctggc caacttgttt cttccaaggc ttgaccaaga aaatcattgc
     2341 ttgctagctc tcgaccatct tcttgacaaa aagtatcaag taaagctatg gccatcccag
     2401 cattctctat ttggtacaaa ccaagcaggc tagtctggaa gcgaccttgt ctgacagcac
     2461 ttgtatagtc aaagacttcc cctgtcacca cactttcttg atgacgaacc tgataatctg
     2521 tcccgtaggc aagtctcggc gcatctttcc cttccgcaat gcggtcaatc acagccaagg
     2581 cttctggagc aatacgccct gttaccaagg gtatcccttg tttgataata cctgccttct
     2641 gctctgctat ggcctccaag gtgtcaccaa gtagagccac atgatccaag ccaatagttg
     2701 taattcctgt caaaatgggc tgacagacat tggtactatc caaaagtcca cccatgccaa
     2761 cttccatgat ggccacatct acttgctctg aggcaaagta gtcataggcc agggctgtga
     2821 taatctcaaa ctcggttgtg ccctgtaaat tggcgaccgc ttctccctcc agcaaagact
     2881 gatagtctgc catgagagct tctagcctcg cttctgagat cgattcccca ttgatgctaa
     2941 tctggtctgt gtaatgaatg aaatagggcg agctaaacac gccaactctc agccctagct
     3001 tttctagcat ctttttcaaa aaagcaatag tcgagccctt gccgttagtc cctccgatat
     3061 ggaggacctt gagtttgaga tggggattgc cacgcaaagc taacagttcc accattcgtt
     3121 ccaatccaaa atgcggttga tccgtccagt agttagcaat ccactgattg ttttcaattt
     3181 ctttcatctt atttatattg ttttaaatct aaattttccg cttcatcagc cagacgaatg
     3241 gcagaggcaa tttcaactgc catcctgtga ctagctacgt catgcacgcg caccacttct
     3301 acaccctgtc tcgcagcgat actggttaca tgagccgaag ccgtgtcccg atttcggaaa
     3361 ccaagctctg tctcaggatt gacttcaaac ccactttctt caaggatatt gatgacaaat
     3421 cgcttgcgcg acactccgag aaagattgga tagcccttct gatgtagttt atccaggtcc
     3481 cgtaaaagga ggagattttc tttcttggtc agaccaaagc caattcctgg atccaacagg
     3541 atattttctg gtgcaatacc agcttctgcc gctctcgcta gtgctcgttc aaagaaagcc
     3601 accatcaagt cttcgattgg caatgtttca aagtcagcta actctttttc tgtaaaggtt
     3661 tgaccaaaac caaaatgagg gaagataagc gaactaggat gctgaggtcg agccataact
     3721 gggttaaaca tgatgaccac tttcgctcca gctttagcaa ccacatgggc cattttttca
     3781 tcacccatga gaccagtgat atcattaact agattggcac ccgcagccaa agcagcctct
     3841 gccacctgac ttttccaagt atcgatagaa ataaggacat cgctttcctt gcgaatcgct
     3901 ttgatcactg gaacaacacg ctggatttcc tcttctatct caacatagct acttcccggc
     3961 cgagtcgatt ctccgccgat atcgagcata ctggctcctt ctgctatcaa tttacgagcc
     4021 tgctggagcg cctgctcaag agcaaaaaat tgaccaccgt ccgaaaagga gtctggggtt
     4081 acattgataa ttccgcaaat aactgtcttt gcatgattgg ctttacttga catatcggtc
     4141 actccctcaa ggattttcat catattattt ttctatttta ccataaaaag aaaaagatgg
     4201 acacgattgc attcatcttt ccctagtaga aagaggtcgg ttcctacata aaaactttta
     4261 aacagaaatt cctaatgtcc gactcataat caccacaaaa gccaacaaac agaaagcaat
     4321 cccattaaca atcatgtgaa gtaagatcga catttccaaa cgttgggtct tgtaggctgt
     4381 ccaagataga actgtcgaca tacctccata aatcaataaa gaaggtaaat tacttggttg
     4441 atgcaataaa gcaaacacaa tcgtaccgac tacaaatccc aagttctcct tgcctcggaa
     4501 aatcttttta ggaacaatcc cacgacacaa gatttcctca caaatcggag caagcaaggc
     4561 tagcaagaag aaactggaaa tcaacgaact attttgaacc atatcattaa tctgagactg
     4621 gttagctgtt gtcgtctcat ttgacagttg caacaagata gaaccaagta tatttgaccc
     4681 gacaataact aaataactca aacccaaacg tgccaaatct ttagctctaa aaaaagaaaa
     4741 attaaaacta gctaacttgg ttttacgagc tcccataata aatagagcca gaaccacaat
     4801 tgaaaggcca gcaaccatca gccctgactg tagcagggct acttctttta aagtcaaaat
     4861 agaggtaacc actaggggaa cctgatataa aaggacggca agtaaaaaga ccaaaatcca
     4921 gcctgcacga ttcaacaatt ctttccacat atttttctct ttcatcacct atctcctttg
     4981 tattaaaaaa ggggggaaat ccccctgggt cattctatta taaggccatg ctaatgtagt
     5041 taaggataaa caaggcatcc aaaatccaaa tcatggcatg aacatcttta acttgacctt
     5101 taacaagctt agtcaaagtg taagtcaaga aaccaactgc aatcccttga gtgatagagt
     5161 agctgaatcc cataaagata gatgtgaaga aggcaggaac tgcttcagac atatcatccc
     5221 aatggatatt tttcaagcta ccaagcatca taatcccaac gataatcaag attggagctg
     5281 tagccgctgt tggtacgatc gctagaagcg ggctaaagaa gcttgaaatc gcaaaacaga
     5341 tagctacaac caaggctgtc aaaccagtac gtccacctgc accgatacca gcagcagact
     5401 caacataagt cgttacgttt gaagtacctg caatggcacc gactgtcgtt ccaatcaaat
     5461 cagagtaaag agccttatcc aatttggctg attggtgatt ttcaccattt gtcgctacaa
     5521 taccaacttt ttcacctgta ccgatcaagg taccaattgt gtcaaaaata tctgtcaatg
     5581 agaaggcaag aatggccatc agagtttcag gcaagcgagc tgtatctgaa accaaagctc
     5641 ccaaaccttc tgaaccaaga gctgcaccaa agattgtctt caaatcttca aaagctgcac
     5701 caacatggtt attagcaaaa tcgatactag acaaatcaac caaaccaact gcaatagcaa
     5761 gaacagttgt tgtcaagata gagagaataa ttcccccttt aatccctttg atgacaaaga
     5821 agatagtaat ggcaagtcct gcaagagcca ctaaaacagc tggattatta aagctgacca
     5881 atcctggaac tgctgaagag tttgctgcaa tcgttgcttg agctttgtca gccccttctc
     5941 ctacaacagt atagttgcct ggatcaatcg tgaatttcaa aagtccagca ttcttaatcc
     6001 ctacataggc aaggaagaca ccgataccag ctgaaatagc tgagcgaaga gcattgggaa
     6061 tcgattcaat gatcatttta cgaacatttg tcaaggtaat aatcaatgaa ataatcccac
     6121 agatgaagac catagctagg gcttcttgcc aagaataacc aagcccgaat acaactgtaa
     6181 aggtaaagaa ggcattgagt cccatacctg gcgcttgggc ataaggtaag ttagcataaa
     6241 aagccatcat caaggtaccc gctactgcac caataatcgt cgctaggaag acgccctgag
     6301 caggcattcc tgtttgtgaa agtatttgtg ggtttacaaa gagaatatag ctcattgcaa
     6361 agaaagttgt taaaccagcg agaacctctg tacgaacgtc tgtaccgttc tcttttagtt
     6421 taaataattt gtccatcatc aatctccttt t
//
LOCUS       DAVMHG010000116         6433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMHG010000116 DAVMHG010000000
VERSION     DAVMHG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.118"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(123..419)
                     /locus_tag="VYR08_001722"
                     /pseudo
     CDS             complement(123..419)
                     /locus_tag="VYR08_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(409..570)
                     /locus_tag="VYR08_001723"
     CDS             complement(409..570)
                     /locus_tag="VYR08_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV3467214.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            640..1404
                     /locus_tag="VYR08_001724"
     CDS             640..1404
                     /locus_tag="VYR08_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV3467215.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            1969..3348
                     /locus_tag="VYR08_001725"
     CDS             1969..3348
                     /locus_tag="VYR08_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV3467216.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3362..4027
                     /locus_tag="VYR08_001726"
     CDS             3362..4027
                     /locus_tag="VYR08_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV3467217.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4162..4320
                     /locus_tag="VYR08_001727"
     CDS             4162..4320
                     /locus_tag="VYR08_001727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467218.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            4382..4921
                     /locus_tag="VYR08_001728"
     CDS             4382..4921
                     /locus_tag="VYR08_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3467219.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            4988..5398
                     /locus_tag="VYR08_001729"
     CDS             4988..5398
                     /locus_tag="VYR08_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV3467220.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5514..>6433
                     /gene="pavB"
                     /locus_tag="VYR08_001730"
     CDS             5514..>6433
                     /gene="pavB"
                     /locus_tag="VYR08_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV3467221.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVK"
BASE COUNT         1749 a         1219 c         1487 g         1978 t
ORIGIN      
        1 atccacaacc ttaaaacagt gttttgagca gcctgcggct aacttcctag tttgcacttt
       61 aattttcatt gagtattact tcatcttctc atgaaattga gtttttgccc agctcctctt
      121 gattattcta cttgaccggg ccaagtattc ataccacctt ctacgttggt aacggtgagg
      181 ccttgggagc tgagaaattg gcaggtagat gcagagcgga ctcctccttg acaaataaca
      241 tagtattcat ggtcaggttt gagttctttg tagccttgct ctaaggtact taatggaaga
      301 tttttggcac ctggtgcatg tcctgcttgg aattcatgtg cttcacggac atcgataagt
      361 tctagatttt cattttagta ttttccataa aagtcagcca tgctgatact agtttccata
      421 ttctactcct tctgggttag ccattttgta tagtgaataa gcgccgtcaa ggttttggac
      481 ggtaaatcct gcttgcttga ggataggctc tgcgatatag ctgcgcaaac cactgtggca
      541 gctgacgatg taggcttgtt ttttgtccag ttcatcagct tcctccttga cactttgtca
      601 gtttttgata caatagtaca aaattagagg aggtgggcta tgattcagaa acatgcgatt
      661 cccattttag agtttgatga taatcctcag gcggttatca tgcccaatca cgaggggttg
      721 gatttgcagt tgccaaagaa gtgtgtctat gcttttttag gtgaggagat tgaccgctat
      781 gcgagggaag taggggcgaa ctgtgttggc gaattcgttt ctgctaccaa gacctatcca
      841 gtctatgtca tcaactacaa gggtgaggag gtctgtctgg ctcaggctcc tgttggctcc
      901 gctccagcag cccagtttat ggattggttg attggctatg gtgtggagca gattatctct
      961 actgggacct gtggtgtcct agctgatata gaggaaaatg cctttctagt ccctgttcgc
     1021 gctttgcgag atgagggagc cagttaccac tatgtggcac cttgtcgtta tatggaaatg
     1081 cagccagagg ctattgctgc tattgaggaa gttttggaag acagagggat tccttatgaa
     1141 gaagtcatga cctggacgac agacggtttt taccgagaaa cggctgaaaa ggtggcttat
     1201 cgtaaggaag aaggctgtgc tgttgtggag atggagtgtt ctgctcttgc ggcagtagct
     1261 caattgcgtg gggttctctg gggtgaattg ttgttcacag cagattctct agcggacttg
     1321 gaccagtacg acagtcgtga ctggggctcg gaagctttta ataaggcgct agaactgagt
     1381 ttagcaagtg ttcaccacct ttagttgtac aggcaaagga tttgttttat cataaaatgt
     1441 ctagctcata cttttcaaaa atatgtttaa acgaggtcac cttcctcttg tcctaggcat
     1501 gttgaggttg ggaaaaatct ttaaaatcag aaaaacgtat catatcaggt gatgaaaatt
     1561 ttgatactat gcgttttatg ttggtaagat ttagagtgag atgaaatgat actcttcgaa
     1621 aatctcttca aaccaggtca gcttcacctt gccgtataga tgttactgac ttcgtcagtt
     1681 ctatctgcaa cctcaaaaca gtgttttgag caacctgtga ctagctttct aagcgatgcc
     1741 ttggttctca ttgcctataa tcaaaaagag aaattttctc ctgaaaagca tatagagtag
     1801 ctggcgttaa aagctcctgt cttgcttttt tgacctatag tcacatctat caagtattgt
     1861 tcttgcctaa gctatcaata aaaaggtggc attttttagg cttggtgtta gtgaattttg
     1921 ccttatccta tctaagtcat ttcgagcttt ttatggtaca atggaaacat gttattcaaa
     1981 ttatctaagg aaaaaataga gctaggctta tctcgtttat cgccagcccg tcgtattttt
     2041 ttgagttttg ccttggtcat tttactaggc tctcttcttt tgagcttgcc ctttgtccaa
     2101 gttgaaagct cacgagcgac ttattttgat catcttttca ctgctgtctc tgcagtctgt
     2161 gtgacaggtc tctcaaccct tccagtagct cacacctata atatctgggg ccaaataatc
     2221 tgtttgctct tgattcagat cggtggtcta gggctcatga cttttattgg ggttttctat
     2281 atccagagca agcaaaagct tagtcttcgt agccgtgcaa ctattcagga tagttttagt
     2341 tatggagaaa ctcgatcttt gagaaagttt gtctattcta tttttctcac gacctttttg
     2401 gttgagagct tgggagctat tttgcttagt tttcgcctta ttcctcaact tggctgggga
     2461 cgtggtcttt ttagttccat ttttctagcg atctcagcct tctgtaatgc cggttttgat
     2521 aatttaggga gcaccagttt atttgctttt cagaccgatt tactggtcaa tctggtgatt
     2581 gcaggcttga ttattacagg cggccttggt tttatggtct ggtttgattt ggctggtcat
     2641 gtaggaagaa agaaaaaagg acgtctgcac tttcatacga agcttgtact attattgact
     2701 ataggtttgt tgttatttgg aacagcaact actctctttc ttgagtggaa caatgctgga
     2761 acgattggca atctccctgt tgccgataag gttttagtta gcttttttca aacagtgacg
     2821 atgcgaacag ctggcttttc tacgatagat tatactcagg ctcatcctgt gactcttttg
     2881 atttatatct tacagatgtt tctaggtggg gcacctggag gaacagctgg gggactcaag
     2941 attacgacat tttttgtcct cttggtcttt gcacgaagtg agcttctagg cttgcctcat
     3001 gccaatgttg cgagacgaac gatcgcgccg cgaacggttc aaaaatcctt tagtgtcttt
     3061 attatctttt tgatgagctt cttgatagga ttgattctgc tagggataac agccaaaggc
     3121 aatcctccct ttatccacct cgtatttgaa accatttcag ctcttagtac agttggtgta
     3181 acggcaaatc tgactcctga ccttgggaaa ttggctctca gtgttatcat gccacttatg
     3241 tttatgggac gaattggtcc cttgaccttg tttgttagct tggcagatta ccatccagaa
     3301 aagaaagata tgattcacta tatgaaagca gatattagta ttggttaaga aaggaaagag
     3361 catgtcagat cgtacgattg gaattttggg cttgggaatt tttgggagca gtgtcctagc
     3421 tgccctagcc aagcaggata tgaatattat cgctattgat gaccacgcag agcgcatcaa
     3481 tcagtttgag ccagttttgg cgcgtggagt gattggtgac atcacagatg aagaattatt
     3541 gagatcagca gggattgata cctgcgatac cgttgtagtc gcgacaggtg aaaatctgga
     3601 gtcgagtgtg cttgcggtta tgcactgtaa gagtttgggg gtaccgactg ttattgctaa
     3661 ggtcaaaagt cagaccgcta agaaagtgct agaaaagatt ggagctgact cggttatctc
     3721 gccagagtat gaaatggggc agtctctagc acagaccatt cttttccata atagtgttga
     3781 tgtctttcag ttggataaaa atgtgtctat cgtggagatg aaaattcctc agtcttgggc
     3841 aggtcaaagt ctgagtaaat tagacctccg tggcaaatac aatctgaata ttttgggttt
     3901 ccgagagcag gaaaattccc cattggatgt tgaatttgga ccagatgacc tcttgaaagc
     3961 agatacctat attttggcag tcatcaacaa ccagtatttg gataccctag tagcattgaa
     4021 ttcgtaaaga gggatgaccc ctcttttttg atgcctaaga tggcaaatag agacagaagc
     4081 cccttgtctt ctagtaaaag ttcttcaaag gctggacttt atggtaaaat agaaagaagt
     4141 gacaagagag agtaatactc aatgaaaatc aaagatcaaa ctaggaaact agctacgggc
     4201 tgctcaaaac actgttttga ggttgtggat agaactgacg aagtcagctc aaaacactgt
     4261 tttgaggttg cagatagaac tgacgaagtc agtaacatct atacggcaag gcgacgttga
     4321 cgcggtttga agagattttc gaagagtata agaaaaaatc agtcccctaa aggagtagat
     4381 tatgaagtta ttgtctatcg caatttctag ctataatgca gcagcctatc ttcattactg
     4441 tgtggagtcg ctagtgattg gtggtgagca agttgggatt ttgattatca atgacgggtc
     4501 tcaggatcag actcaggaaa tcgctgagtg tttagctagc aagtatccta atatcgttag
     4561 agccatctat caggaaaata aatgccatgg cggtgcggtc aatcgtggct tggcagaggc
     4621 ttctgggcgc tattttaaag tagttgacag tgatgactgg gtggatcctc gtgcctactt
     4681 gaaaattctt gaaaccttgc aggaacttga gagcaaaggt caagaggtgg atgtctttgt
     4741 gaccaatttt gtctatgaaa aggaagggca gtctcgtaag aagagtatga gttacgattc
     4801 agtcttgcct gttcggcaga tttttggctg ggaccaggtc ggaaatttct ccaaaggcca
     4861 gtataccatg atgcactcgc tgatttatcg gacagatttg ttgcgtgcta gccagttcta
     4921 actgcctgaa catacttttt atgtcgataa tctctttgtc tttacgcccc ttcagcaggt
     4981 caagaccatg tactatctgc ctgtcgattt ctatcgttat ttgattgggc gtgaggacca
     5041 gtctgtcaat gagcaagtga tgattaagtg cattgaccag caactcaagg tcaatcgact
     5101 cttggtagac caacttgatt tgtcccaagt gagtcatccc aaaatgcgag aatatctgct
     5161 gaatcatatt gaaatcacga cggtgatttc cagtaccctg ctcaaccgat ctggaacagc
     5221 ggagcatctg gcaaaaaaac gccaattgtg gacctatatt cagcagaaaa atccagaagt
     5281 ctttcaggct attcgtaaga ccatgttgag ccgtttgacc aaacattctg tcttgccaga
     5341 tcgcaaactg tccaatgtcg tctatcaaat caccaaatct gtttatggat ttaattaata
     5401 taagtgtttt ataagaggga tttaagaaaa attttaactt tttcttagtc ctttttaatt
     5461 tcaggagatt atactagagt catcaaataa agaaagactc taaggagaat cctatgaaat
     5521 tcaatccaaa tcaaagatat actcgttggt ctattcgccg tctcagtgtc ggtgttgcct
     5581 cagttgttgt ggctagtggc ttctttgtcc tagttggtca gccaagttct gtacgtgccg
     5641 atgtggtcaa tccgacccca ggtcaagtct tacctgaaga gacatcggga acgaaagagg
     5701 gtgacttatc agaaaaacca ggagacaccg ttctcactca agcgaaacct gagggcgtta
     5761 ctggaaatac gaattcactt ccgacaccta cagaaagaac tgaagtgagc gaggaaacaa
     5821 actcttctag tctggataca ctttttgaaa aagatgaaga agctcaaaaa aatccagagc
     5881 taacagatgt cttaaaagaa actgtagata cagctgatgt ggatgggaca caagcaagtc
     5941 cagcagaaac tactcctgaa caagtaaaag gtggagtgaa agaaaataca aaagacagca
     6001 tcgatgttcc tgctgcttat cttgaaaaag ctgaagggaa aggtcctttc actgccggtg
     6061 taaaccaagt aattccttat gaactattcg ctggtgatgg tatgttaact cgtctattac
     6121 taaaagcttc ggataatgct ccttggtctg acaatgggac tgctaaaaat cctgctttac
     6181 ctcctcttga aggattaaca aaagggaaat acttctatga agtagactta aatggcaata
     6241 ctgttggtaa acaaggtcaa gctttaattg atcaacttcg cgctaatggt actcaaactt
     6301 ataaagctac tgttaaagtt tacggaaata aagacggtaa agctgacttg actaatctag
     6361 ttgctactaa aaatgtagac atcaacatca atggattagt tgctaaagaa actgtcgaaa
     6421 aagctgttaa aga
//
LOCUS       DAVMHG010000117         6393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMHG010000117 DAVMHG010000000
VERSION     DAVMHG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.119"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            38..433
                     /locus_tag="VYR08_001731"
     CDS             38..433
                     /locus_tag="VYR08_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV3467222.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            444..815
                     /locus_tag="VYR08_001732"
     CDS             444..815
                     /locus_tag="VYR08_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV3467223.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            825..3068
                     /locus_tag="VYR08_001733"
     CDS             825..3068
                     /locus_tag="VYR08_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV3467224.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3275..5050
                     /gene="spxB"
                     /locus_tag="VYR08_001734"
     CDS             3275..5050
                     /gene="spxB"
                     /locus_tag="VYR08_001734"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV3467225.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5161..5508
                     /locus_tag="VYR08_001735"
     CDS             5161..5508
                     /locus_tag="VYR08_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV3467226.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5625..6393)
                     /locus_tag="VYR08_001736"
                     /pseudo
     CDS             complement(5625..6393)
                     /locus_tag="VYR08_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1893 a         1219 c         1433 g         1848 t
ORIGIN      
        1 agtgtaattg acaaatgtag attttggagg tatggtaatg cagatttcag atgcagaatg
       61 gcaggtcatg aagattattt ggatgcaggg ggagcagacc agtacagatt tgattagggt
      121 tttggcagag cggtttgact ggtccaagtc cacggttcag acacttttgg ctcgtctggt
      181 tgagaaagag tgtttgactc ggaaaaaaga aggcaagtcc tttgtttatt cagccctttt
      241 aactctagac caaagtcggg atttacttgt ccaagatatc aaagacaagg tttgttcccg
      301 taggattagg aatttgttgg ctgacttgat tgtagaatgt gaatttactc agactgactt
      361 ggaagacttg gaagctgtga tttcagagaa gaaatcaagt gctgtaacag aagtaagatg
      421 taattgtatg taaaggagac gtcatgttaa atagtattgt aaccattatt tgtattgccc
      481 ttatcgcatt tatcttgttt tggtttttca aaaagcctga aaaatctgga caaaaagccc
      541 agcaaaaaaa cggataccaa gagattcgag tggaagtcat gggaggctat actcctgagt
      601 tgattgtcct aaagaaatca gtgccagcac gcattgtctt tgaccgcaag gatccttcac
      661 catgtctgga tcaaattgtt tttccagatt ttggtgtaca tgcgaacctg ccaatggggg
      721 aagagtatgt agtggaaatc acgcctgaac aggctggaga gtttggcttt gcttgtggta
      781 tgaacatgat gcacggcaag atgattgtag agtaggtgga gactatgaca gaaattgtga
      841 aagcaagctt agaaaatggc attcaaaaaa tccgtatccg agctgaaaaa ggctatcatc
      901 cagcccatat ccagcttcaa aagggaattc cagctgagat tacctttcat cgtgctactc
      961 cttcaaactg ttataaggaa attctgtttg aagaagaagg tatcttggaa ccaatcggcg
     1021 tagatgagga gaaagtcatt cgttttacgc ctcaagaatt agggcgacat gaattttctt
     1081 gtggcatgaa gatgcaaaag ggaagctata cagtcgttga gaagactcga aaatctctat
     1141 ctctcctgca acgtttttgg attactagta tctttactgt gcctcttgtg attctcatga
     1201 ttgggatgtt ggcaggtagc attagtcatc aagtcatgca ttggggaacc tttttagcaa
     1261 caacgcctat tatgttagtt gcaggtaagc catatatcca gagtgcttgg gccagtttta
     1321 aaaagcacaa tgccaacatg gataccttgg ttgcgctggg aactctagtg gcttatttct
     1381 atagcctagt tgctctcttt gctggtctcc ctgtttactt cgaaagtgct ggatttatcc
     1441 tctttttcgt tcttttggga gcagtttttg aggaaaaaat gagaaaaaat acgtcccaag
     1501 ctgtggagaa attactggac ttgcaagcta aaaccgcaga agtcttgagt gatgatagtt
     1561 atgtccaagt tcctttggaa caagtcaagg taggcgacct gattcgagtg cgtcccggtg
     1621 aaaagattgc tgttgatggt gtcgtagtag aaggtgtctc tagtattgac gaatccatgg
     1681 tgacaggtga gagtctgcct gtggacaaga cagttggaga tactgtcatt ggctcaacca
     1741 tcaatcatag tggaacgctt gtctttagag cagaaaaagt tggctcagag actgttttgg
     1801 ctcagattgt ggattttgtg aagaaagctc agacaagtcg tgcgccgatt caggacttga
     1861 cggataagat ttcagggatt tttgtcccag tagttgtcat tttaggaatc atgacctttt
     1921 gggtttggtt cgtcttgctc agggatagtg tggtcgtgct tggagctagc tttgtgtcct
     1981 ctcttctcta cggagtggcg gttttgatta tcgcctgtcc ttgtgccttg ggacttgcaa
     2041 caccgacagc ccttatggtg gggacaggac gtagtgccaa gatgggggtt ctcctcaaaa
     2101 atggaactgt cttacaggaa atccagaaag ttcaaactct tgtctttgat aagaccggga
     2161 ctttgacgga agggaaacct gtggtaacag atatcatcgg tgacgaagta gaagtgtttg
     2221 gattggcagc ctccttggaa gatgcttctc aacacccact ggctgaggct atcgttaagc
     2281 gagcgagtga agctggactt gagtttcaaa ctgttgaaaa ttttcaggcc ttgcacggga
     2341 aaggtgtttc agggcgaatc aatggaaaac aagttttact tggaaatgct aaaatgctgg
     2401 atggcatgga tatttctaat acttatcaag ataaactaga agaactagaa aaagaagcta
     2461 agacagttgt gtttttagct gttgacaatg aaatcaaagg cttgcttgct ttgcaagata
     2521 ttcctaagga aaatgctaag ctagccatca gtcagctaaa aaaacgtggt ctccgaacag
     2581 tcatgctgac aggagacaat gctggtgtgg cgcgtgctat tgcagatcaa atcggaattg
     2641 aagaggtcat tgcaggcgtc ttgccagaag aaaaagccca tgaaatccat aaactgcaac
     2701 agtcagggaa agtagccttt gttggggacg gtatcaatga cgctcctgcc cttagtgtag
     2761 cagatgtggg gattgctatg ggagctggaa cagatatcgc catcgagtca gcagatttgg
     2821 tgttgacaac caataatctt ttaggagtgg ttcgtgcctt tgatatgagt aagaaaacct
     2881 ttcatcgaat tctactcaat cttttctggg cttttatcta caatgttgtc ggaattccga
     2941 ttgcagcagg agtcttttca ggtgttggac tggctctcaa cccagaattg gcaggtctag
     3001 ccatggcctt tagttctgta tctgttctga ccagttccct tctgttaaat tttagtaaaa
     3061 tagactaaaa gtaatgacta gatttctttg ttataaaaca gaaatgacag aactttaaaa
     3121 actgtattag aatagagaat agagagtttt gagcagattt ttagaaaagt cagcataaat
     3181 atgatacagt ggaatagtaa aaatttggag aacgtttcca attctatgta atcgtattct
     3241 ccaaatctaa aaaaattgaa ggagagttat cattatgact caagggaaaa ttactgcatc
     3301 tgcagcaatg cttaacgtat tgaaaacatg gggcgtagat acaatctacg gtatcccatc
     3361 aggaacactc agctcattga tggacgcttt ggctgaagac aaagatatcc gcttcttaca
     3421 agttcgccac gaagagacag gtgctcttgc agcggttatg caagctaaat tcggcggctc
     3481 aatcggggtt gcagttggtt caggtggtcc aggtgcgact cacttgatta acggtgttta
     3541 cgatgcagct atggataaca ctccattcct agcgatcctt ggatcacgtc cagttaacga
     3601 attgaacatg gatgctttcc aagagcttaa ccaaaaccca atgtacaacg gtatcgctgt
     3661 ttacaacaaa cgtgtagctt acgctgagca attgccaaaa gtaattgacg aagcctgccg
     3721 tgctgcaatt tctaaaaaag gtccagctgt tgttgaaatt ccagtaaact tcggtttcca
     3781 agaaatcgac gaaaactcat actacggttc aggttcatac gaacgctcat tcatcgctcc
     3841 tgctttgaac gaagttgaaa tcgacaaagc tgttgaaatc ttgaacaatg ctgaacgccc
     3901 agttatctat gctggatttg gtggtgttaa agctggtgaa gtgattactg aattgtcacg
     3961 taaaatcaaa gcaccaatca tcacaactgg taaaaacttt gaagctttcg aatggaacta
     4021 tgaaggtttg acaggttctg cttatcgtgt tggttggaaa ccagccaacg aagtggtctt
     4081 tgaagcagac acagttcttt tccttggttc aaacttccca tttgctgaag tttacgaagc
     4141 attcaagaac actgaaaaat tcatccaagt tgatatcgac ccttacaaac ttggtaaacg
     4201 tcatgccctt gacgcttcaa tccttggtga tgctggtcaa gcagctaaag ctatccttga
     4261 caaagtaaac ccagttgaat caactccatg gtggcgtgca aacgttaaga acaaccaaaa
     4321 ctggcgtgat tacatgaaca aactcgaagg taaaactgag ggtgaattgc aattgtatca
     4381 agtttacaat gcaatcaaca aacatgctga tcaagacgct atctactcaa tcgacgtagg
     4441 taacactact caaacatcta ctcgtcacct tcacatgaca cctaagaaca tgtggcgtac
     4501 atctccactc tttgcgacaa tgggtattgc ccttcctggt ggtatcgctg ctaagaaaga
     4561 caatccagat cgccaagtat ggaacatcat gggtgacgga gcattcaaca tgtgctaccc
     4621 agacgttatc acaaacgttc aatacgacct tccagttatc aaccttgtct tctcaaatgc
     4681 tgagtacggc ttcatcaaga acaaatacga agatacaaac aaacacttgt ttggtgtaga
     4741 cttcacaaac gctgactacg ctaaaattgc ggaagctcaa ggagctgttg gattcacagt
     4801 tgaccgtatc gaagacatcg atgcagttgt tgcagaagct gttaaattga acaaagaagg
     4861 taaaactgtt gtcatcgatg ctcgcatcac tcaacaccgt ccacttccag tagaagtact
     4921 tgaattggat ccaaaacttc actcagaaga agctatcaaa gccttcaagg aaaaatacga
     4981 agcagaagaa ctcgtaccat tccgtctctt cttggaagaa gaaggattgc aatcacgcgc
     5041 aattaaataa ttcctctcgc cgaaaatcaa atatgaaact tgtagacttt attctttgat
     5101 tttcatagag cagaacttta aaagatcgga gcaatccggt ctttttatgg aggagagtaa
     5161 atgaatttaa atcaattaga tattatcgtt tcaaatgttc cccaagtctg tgctgacttg
     5221 gagcatattt tggataaaaa ggcagattat gctgatgatg gttttgctca gtttacgatt
     5281 ggtagtcact gccttatgtt gtcacaaaat catttggttc ctttggaaaa ctttcagtca
     5341 ggaatcatta ttcatatcga ggttgaggat gtagaccaga actacaaacg gttgaacgag
     5401 cttggtataa aggttttaca cggaccaact gtaaccgatt ggggaacaga gtccttatta
     5461 gttcaaggac ctgctggtct agtgcttgat ttttatcgta tgaaataggt cgcgtagtca
     5521 tttgacgtta accttagcta tttttaaaat agaaattgag aatatgaact attgaaagat
     5581 cggagcaatc cggtcttttt atataaagaa attgcatata atactcatat gctttcaaaa
     5641 agcattctag tccatctccg attaacgatg gactttatca cctccttctc cagtccttgt
     5701 atgacatctt gaagttgatt catgacatct tccaaagttc gaaaggcctt attcttaaat
     5761 ccacgtttac gaatctcttt ccacacttgt tcaatgggtt catctctggt gtgtatggag
     5821 gaataaaggt aaaaccaata ttagtcggaa tctttaaggt acttgattta tgccatatag
     5881 cattgtccat aacgagtaaa agataatcat ctggataagc ttgtgaaagc tcttctaaaa
     5941 aggcgttcat ccactcagta ttacatccac cagctattaa gaaaaatgat tcgcctgtat
     6001 gggcatcaac agctccataa caatagtgaa attctcgtat atagtgacta tggacatgtg
     6061 gacctactcc tattggagcc caacaagatc ccagtttact gattctaccg aaaccagcct
     6121 catcttggta catcaagcga accttacgaa aacgtctact gcttttaaag cgctttcttt
     6181 tcttcttgaa ttgagatttt atttttagac gcgacaatgg tttgagcgtc tgctttccta
     6241 ggatgttctg gacgtggcat aatatttcgc caaccatggc gcttcaacag ttgatagaag
     6301 gcatcacgtg tgtaggaacg acctaactcc tttttataag cctgaaataa ggcatcaatt
     6361 gtaacaaatt ctcctgcctc tgtagccttc aaa
//
LOCUS       DAVMHG010000118         6271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMHG010000118 DAVMHG010000000
VERSION     DAVMHG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.120"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            11..1711
                     /locus_tag="VYR08_001737"
     CDS             11..1711
                     /locus_tag="VYR08_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV3467227.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1704..2180
                     /gene="ilvN"
                     /locus_tag="VYR08_001738"
     CDS             1704..2180
                     /gene="ilvN"
                     /locus_tag="VYR08_001738"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV3467228.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2246..3268
                     /gene="ilvC"
                     /locus_tag="VYR08_001739"
     CDS             2246..3268
                     /gene="ilvC"
                     /locus_tag="VYR08_001739"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV3467229.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3375..3611
                     /locus_tag="VYR08_001740"
     CDS             3375..3611
                     /locus_tag="VYR08_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467230.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3849..4204
                     /locus_tag="VYR08_001741"
                     /pseudo
     CDS             3849..4204
                     /locus_tag="VYR08_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            4254..5504
                     /gene="ilvA"
                     /locus_tag="VYR08_001742"
     CDS             4254..5504
                     /gene="ilvA"
                     /locus_tag="VYR08_001742"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV3467231.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLIGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1937 a         1050 c         1428 g         1856 t
ORIGIN      
        1 aaggggaaca atggagaaaa tcagtttaga atctcctaag acggggtcgg acctagtttt
       61 ggaaacactt cgtgatttag gagttgatac catctttggt tatcctggtg gtgcggtttt
      121 gcctttttat gatgcgatat ataattttaa aggcattcgc cacattctag ggcgccatga
      181 gcaaggttgt ttgcatgaag ctgaaggtta tgccaaatca actggaaagt tgggtgttgc
      241 cgtcgtcact agtggaccag gagcaacaaa tgccattaca gggattgcgg atgccatgag
      301 cgatagcgtt ccccttttgg tctttacagg tcaggtggcg cgagcaggga ttgggaagga
      361 tgcctttcag gaggcagaca tcgtgggaat taccatgcca atcactaagt acaattacca
      421 agttcgtgag acagctgata ttccgcgtat cattacggaa gctgtccata tcgcaactac
      481 aggccgtcca gggccagttg taattgacct accaaaagac atatctgctt tagaaacaga
      541 cttcatttat tcaccagaag tgaatttacc aagttatcag ccgactcttg agccgaatga
      601 tatgcaaatc aagaaaatct tgaagcaatt gtccaaggct aaaaagccag tcttgttagc
      661 tggtggtgga attagttatg ctgaggctgc tacggaacta aatgaatttg cagaacgcta
      721 tcaaattcca gtggtaacca gtcttttggg acaaggaacg attgcaacga gtcacccact
      781 ctttcttgga atgggaggca tgcacgggtc attcgcagca aatattgcca tgacggaagc
      841 ggactttatg attagtattg gttctcgttt cgatgaccgt ttgacgggga atcctaagac
      901 tttcgctaag aatgctaagg ttgcccacat tgatattgac ccagctgaga ttggcaagat
      961 tatcagtgca gacattcctg tagttggaga tgctaagaag gccttgcaaa tgttgctagc
     1021 agaaccaaca gttcacaaca atactgaaaa gtggattgag aaagtcacta aagacaagaa
     1081 tcgtgttcgt tcttatgaca agaaagagcg tgtggttcaa ccgcaagcag ttattgaacg
     1141 aattggtgaa ttgacgaatg gagatgccat tgtggtaaca gacgttggtc aacaccaaat
     1201 gtggacagct cagtattatc cctaccaaaa tgaacgtcag ttagtgactt caggtggttt
     1261 gggaacaatg ggctttggaa ttccagcagc aatcggtgct aaaattgcta acccagataa
     1321 ggaagtagtc ttgtttgttg gggatggtgg tttccaaatg accaaccagg agttggctat
     1381 tttgaatatt tacaaggtgc caatcaaggt ggttatgctg aacaatcatt cactcggaat
     1441 ggttcgccag tggcaggaat ccttctatga aggcagaaca tcagagtcgg tctttgatac
     1501 ccttcctgat ttccaattga tggcgcaggc ttatggtatt aaaaactata agtttgacaa
     1561 tcctgagacc ttggctcaag accttgaagt catcactgag gatgttccta tgctaattga
     1621 ggtagatatt tctcgtaagg aacaggtgtt accaatggta ccggctggta agagtaatca
     1681 tgagatgttg ggggtgcagt tccatgcgta gaatgttaac agcaaaacta caaaatcgtt
     1741 caggagtcct caatcgcttt acaggtgtcc tatctcgtcg tcaggttaat attgaaagca
     1801 tctctgttgg agcaacagaa gatccgaatg tatcgcgtat cactattatt attgatgttg
     1861 cttctcatga tgaagtggag caaatcatca aacagctcaa tcgtcagatt gatgtgattc
     1921 gcattcgaga tattacagac aagcctcatt tggagcgcga ggtgattttg gttaagatgt
     1981 cagcgccagc tgagaagaga gctgagattt tagcgattat tcaacctttc cgtgcaacag
     2041 tggtagacgt agcgccgagc tcgattacca ttcagatgac gggaaatgca gaaaagagcg
     2101 aagccctatt gcgagtcatt cgcccatacg gtattcgcaa tattgctcga acgggtgcaa
     2161 ctggatttac ccgcgattaa aaatccaact taaatttatt aaaccagcct aaaaggcaat
     2221 aaataataga aaagagagaa aagctatgac agttcaaatg gaatatgaaa aagatgttaa
     2281 agtagcagca cttgacggta aaaaaatcgc cgttatcggt tatggttcac aagggcatgc
     2341 gcatgctcaa aacttgcgtg attcaggtcg tgacgttatt atcggtgtac gtccaggtaa
     2401 atcttttgat aaagcaaaag aagatggatt tgatacttac acagtagcag aagctactaa
     2461 gttggctgat gttatcatga tcttggcgcc agacgaaatt caacaagaat tgtacgaagc
     2521 agaaatcgct ccaagcttgg aagctggaaa cgcagttgga tttgcccatg gtttcaacat
     2581 ccactttgaa tttatcaaag ttcctgcgga tgtagatgtc ttcatgtgtg ctcctaaagg
     2641 accaggacac ttggtacgtc gtacttacga agaaggattt ggtgttccag ctctttatgc
     2701 agtataccaa gatgcaacag gaaatgctaa aaacattgct atggactggt gtaaaggtgt
     2761 tggagcggct cgtgtaggtc ttcttgaaac aacttacaaa gaagaaactg aagaagattt
     2821 gtttggtgaa caagctgtac tttgtggtgg tttgactgcc cttatcgaag caggtttcga
     2881 agtcttgaca gaagcaggtt acgctccaga attggcttac tttgaagttc ttcacgaaat
     2941 gaaattgatc gttgacttga tctacgaagg tggattcaag aaaatgcgtc aatctatttc
     3001 aaacactgct gaatacggtg actatgtatc aggtccacgt gtaatcactg aacaagttaa
     3061 agaaaatatg aaggctgtct tggcagacat ccaaaatggt aaatttgcaa atgactttgt
     3121 aaatgactat aaagctggac gtccaaaatt gactgcttac cgtgaacaag cagctaacct
     3181 tgaaattgaa aaagttggtg cagaattgcg taaagcaatg ccattcgttg gtaaaaacga
     3241 cgatgatgca ttcaaaatct ataactaatt agaaatatat agcgctggag atgattttat
     3301 gaaaaagatt atgagaaaaa ctgcatcgtt attattggtt ctagttgtat aatgtaatta
     3361 caccgtcggt aatagtgcta gcagaccaaa ataaagcaga ttggtcgtat gatgaaaatg
     3421 ctgtaattaa catttatgat gatgctaatt ttgaagatgg taggttgcat atgacctttg
     3481 aacaattctt caaattggca caaatagcta gagaagaagg tcttgaaatt cattctccgt
     3541 ttgagagagc tggtgcgact aaatctgctc gttatatagc gaaatggatt ttgagaaata
     3601 aaaaacatta acaaatatag ttggtaaatc attaggacct aaatcagctg ttagattcgg
     3661 agaagcttta tcctatattg aaggtcctct tcgcagaata aatgagacga tagatggcgg
     3721 tttatatcaa atagagcaaa ttattgcatc tggattgaaa gaatcgggtt taaatgactg
     3781 gactgcgaaa actttagctt cagctattcg tgggatatta gatgtactta tttaggggtt
     3841 gaaatcatat gaatattacc aatttgtttt ctatcaagac aggatgtgat aaactgatag
     3901 gcaactgcaa aaactatttt ttcagttgga tttacaattg ggagaattga cagatcaact
     3961 aagaaaatta gattctaatt ttgttcctcg tagtcaattt gtagacacgt tggatttgaa
     4021 tgatgtagaa tataaagaaa ttttaaacta ttttatcttc catcgtaatg atagtgaaga
     4081 aagtttggta gaatggttat atgattggat ttccacaaat cgttatgaac ttcctaaaga
     4141 gttttcgatt cgtatggctc ataaatacca tgaaagtgtt actgaagttt tcggagatga
     4201 ataactaaaa aacagtcatt agtgactgtt ttttatagaa aaagaggttt tatatgttaa
     4261 gttcaaaaga tataatcaag gctcacaagg tcttgaacgg tgtggttgtg aatactccac
     4321 tggattacga tcattattta tcggagaagt atggtgctaa gatttatttg aaaaaagaaa
     4381 atgcccagcg tgttcgctcc tttaaaattc gtggtgccta ttatgccatt tcccagctca
     4441 gcaaggaaga acgtgaacgt ggggtagtct gcgcttctgc gggaaatcat gcgcagggag
     4501 tagcctatac ttgtaatgaa atgaaaattc ctgctactat ctttatgcct attactacgc
     4561 cacaacaaaa gattggtcag gttcgctttt ttggtgggga ttttgtaact attaaactaa
     4621 ttggagatac ctttgatgcc tcagccaaag cagctcaaga atttacagtc tctgaaaatc
     4681 gtacctttat tgatcctttt gatgatgctc atgttcaagc aggtcaagga acagttgctt
     4741 atgagatttt agaagaagct cgaaaagaat cgattgattt tgatgctgtc ttggttcctg
     4801 ttggtggtgg cggtctcatt gccggggttt ctacctatat caaggaaaca agtccagaga
     4861 ttgaggttat cggagtagag gcgaatggag cgcgttccat gaaagctgcc tttgaggctg
     4921 gaggtccagt caaactcaag gaaattgata aatttgcgga tgggattgct gtgcaaaagg
     4981 taggtcagtt gacctatgaa gcaactcgtc aacatattca aactttggta ggtgtcgatg
     5041 agggattgat ttctgaaacc ttgattgacc tttactctaa gcaagggata gtcgcagaac
     5101 ctgctggagc agctagtatc gcctctttag aggttttagc tgaatatatt aaggggaaaa
     5161 ccatttgttg tatcatttct ggaggaaata atgatatcaa ccgtatgcca gaaatggaag
     5221 agcgtgcctt gatttatgat ggaatcaaac attattttgt ggtcaatttc ccacaacgtc
     5281 caggagcttt gcgtgagttt gtaaatgata tcctggggcc aaatgatgat atcacacgtt
     5341 ttgagtatat caaacgagct agcaagggaa caggcccagt attgattggg atcgctttag
     5401 cagataagca tgattatgca ggtttgattc gtagaatgga aggttttgat ccagcttata
     5461 ttaacttaaa tggtaatgaa acgctttata atatgcttgt ctgaggacta ataaaaaaat
     5521 atcatattat ttgtacaact gatgtatatg tgctataatg aagatgaaga aaggggacga
     5581 ttcctatggt tttagaaaac ttatcgtgca attcttactt ctgttcatta tagcacttat
     5641 gatgtttggc tcagatttcc atccttcatt ttgatttcct atctattgac aagcatagtc
     5701 acactgtctt taatactctt cgaaaatctc ttcaaaccac gttagctcta tctgcaacct
     5761 caaaacagtg ttttgagcaa cttgcggcta gcttcctagt ttgctctttg attttcattg
     5821 agtataaggt atgatttgat ttctttttgt tgacaaatat accatattaa aaagatatat
     5881 aagtaattaa ctgagcttat ctgtcttgtc atctctatta aggatggttt agataatcgg
     5941 gtgtctgctt ctaggctagc acctcaatat ccaaaggagt gatgaatttg aaggatataa
     6001 ggaataccta tctctcagat gatttattga ggaagaaaga taggagtttt tgagctagtg
     6061 aaggcttgga tttctaaagg ttagaactat catcttcagt tcttaaatcg aagaaataag
     6121 ctatcttacg gaaatagaga agcatttttt aagaacttga ataatttcgc accttaagag
     6181 ggtaataata cagtagtttt attagcaaat atttatggtg tagaggctag caaaacctat
     6241 atattatcgg atttaaaaag gaagtaagaa a
//
LOCUS       DAVMHG010000119         6208 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMHG010000119 DAVMHG010000000
VERSION     DAVMHG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.121"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(35..529)
                     /locus_tag="VYR08_001743"
     CDS             complement(35..529)
                     /locus_tag="VYR08_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467232.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(526..990)
                     /locus_tag="VYR08_001744"
     CDS             complement(526..990)
                     /locus_tag="VYR08_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467233.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(1000..2334)
                     /locus_tag="VYR08_001745"
     CDS             complement(1000..2334)
                     /locus_tag="VYR08_001745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV3467234.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGSDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(2345..2962)
                     /locus_tag="VYR08_001746"
     CDS             complement(2345..2962)
                     /locus_tag="VYR08_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3467235.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSTPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(3010..6108)
                     /locus_tag="VYR08_001747"
     CDS             complement(3010..6108)
                     /locus_tag="VYR08_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3467236.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFAVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
BASE COUNT         1732 a         1438 c         1198 g         1840 t
ORIGIN      
        1 tccaaaaaaa gcaatctatg atacttttcc taacttattt tttcatttcc cagaccatat
       61 cataccatgt ttcccctgca aaggttgact gggactggcc ttcgttgaca aatccatgtt
      121 tttcataata ggcgatgaga tagtcatgac aggttagatt aatgccatct ctctcatctt
      181 caagagcaac ttctttcaag gctgtcagca atttctgacc cagtccgagt ccctgtgcct
      241 ccttggcaat agacagacag gtcacagaga tataaccacc aggctcatga ctatagtctt
      301 ttatttcttc tgtaaaagac tggtcttgca gatggcggtg caggccaact ggtccttcga
      361 tataacccat gattcttcct tctttttcag caaccagaaa agaggtctga atttctctca
      421 aatgtgcttc aaagacagaa ggaggaatgg cttcttcgac cgaaaaattt tcaaattcaa
      481 gttcaacaat ccgatccaaa tcttctaatc ttgcttgtct gattttcatt gttcctccag
      541 ataaaaggga ttaaaccaaa tcatactata gccctggcta gttgcataga gcaaagttgc
      601 ttcttcatca acaaaaccgt tcatttcaaa ataggaaagc agctcatcag gactctccaa
      661 acgaatcact ttgtaatcca gctcaactgc cacctctttc aaggctgcaa gaagaagtgt
      721 tcccaggccc tgtctctgat ggtcagactc gatgactaaa gaatgtactt ttagacattg
      781 cggattgtct gactggggac ttgataaaat atagcctaaa agttgatttt catccctagc
      841 tagaagaaag gtatccgcac acttacggat actttcttct aaaatatggg aaagttgctg
      901 cttttcagct ggaaaagacg aggtctgaag tgcccctatc tcaggcaaat cagacttgct
      961 tgcctgaatg atcttaattg gaatttccat gggaacatcc tattgaacat tgcttgtcaa
     1021 gttagacaag agacgctcaa atgagtattc ataggtttgg atgtctcctg ctcccataaa
     1081 gacgtaaaca gcattgtcat ggtctaggag tggagaaaca ttttcaacag taatcacttg
     1141 gtgttttttg ttgattttgt tggctaggtc ttctacctta acgtcaccat gatctacttc
     1201 acgagccgag ccataaattt gcgctagata aacagcatct gcttggttta aagcatgggc
     1261 aaagtcgtcc aacaaggcaa tggttcttgt aaaggtatgc ggttgaaaga ctgctacaat
     1321 ttccttgctt gggtatttct gacgagccgc atccaaggtc gcaataattt ctgttggatg
     1381 atgggcaaag tcatcaataa tcactgtatc attgacaatt ttctcagtga aacgacgttt
     1441 aacaccggca aatgttttca agtgctcacg caccaagttc aaatcaaatc ctgctgtgta
     1501 aagaagacca ataacggctg tcgcattcat gatattgtga cgaccaaagg ttggaatgtg
     1561 gaattgcccc aagttttgtc cacggaaatg aacggtgaag gttgaaccag ttgttgaacg
     1621 aagaagatca ctagctacaa agtcactgcc ttcagcttca aaaccataat aataaattgg
     1681 tgcatcagac gtaatcttac gcaattcagc atcttcacca tagacaaaaa gacccttggt
     1741 aatttgtttg gcatagtcgt taaaggcatt gaaaacatcc tcgagacttg tgaaataatc
     1801 tggatggtca aagtcaatgt tggtgataat agagtattct gggtggtaag gcatgaagtg
     1861 acgctcatat tcgtcagatt caaagacaaa atatttggca ttagccgaac cacgacctgt
     1921 cccatctcca atcaagaagc tggtatctgt aatgtgagac aagacatgag acaacatacc
     1981 tgtcgttgaa gtttttccat gtgctcctgc tactcccatg ctaacaaagt cacgcataaa
     2041 gctacctaga aactcatggt aacgtttgta gctgatacca ttttggtccg cataggcaat
     2101 ttcgacgttg ttatctggac gaaaggcatt tccagcgata atttccatat caccgtctag
     2161 attcttttca tcaaaaggaa gaatggtaat tcctgcctgc tcaagaccgc gttgggtaaa
     2221 gtagtacttt tcaacatctg atccctgaac cttgtgcccc atctggtgca acatcaaggc
     2281 caaggcactc atccctgatc ccttaattcc gataaaatga tatgtctttg acatgttttc
     2341 tcccctattc tgtcattctg gtcagattca actcttgggc aacccgacgt tcttgttctg
     2401 tttgtttact ttttttattg tagatttggc tcttctttag aaaatcataa ttgtttttct
     2461 ttggagcagg tgttgacact tcttcattct tggtagggat agaatgaact tcttccgcca
     2521 agatataatg agactgggtc aatttttggc tatatttgac aaattcacca ggattttcct
     2581 tttggaaagg agttgtcggt tgattgccct gtctaactag actgggctga gaatgacgtc
     2641 tcgcaaggct gaaatcctga gttaggtagt tagcagagcg tttctttttc aagtccgcac
     2701 gcgcttcttc acgcgccacc tccgcatagc tctttccttc ttttttaacc cctaaaggag
     2761 cctttttagg tttttcgact tgcttttcaa tcggttttac tggtttttct tcagtaatag
     2821 gagcccattc taaataattt ttatctcgat actcaccctt gatattactg atcagatcag
     2881 actcatcata gagattcatg actggcattt cagtcaacat gacctcgtca tctgacacca
     2941 atggaaatcg ttcttgtttc attttctatt tcctttcaac acttcattat agcgtattgt
     3001 cttgattttt caagtgctgg cttcagaaat tcccaaaatt tctctaattt ctgctagggt
     3061 cagactacca cgtgactctg tgccatccaa tacttgtgac accagatgtt tcttttgttc
     3121 ttggagttcc tgaatttttt cttcaatggt tcccttggtc accaagcgat agacctcaac
     3181 cgtttcttcc tgacccatcc gatgggcacg gccaatggct tgcgcttcca ccgcaggatt
     3241 ccaccaaagg tcaaccaaga tcactgtatc tgcacctgtc aggttcagac cgaccccacc
     3301 agccttgagg gaaatcagaa aggcatctct ttctccttgg ttaaaggcct tggtcatgtc
     3361 ttgtctttcc ttggctgggg ttgaacccgt aattttaaag gatgtcaggc ccaagtctgg
     3421 cagttcttgt tcaatttttt ccaacattcc cttgaactga gagaaaatca agacacggtg
     3481 tccgccgtct gccacctgta ccagtaggtc tcggagacta tctagtttgc cgctggctcc
     3541 ctgataatct tccataaaca gggcaggagt gtcacatatt tgacgcaagc gcatcaaacc
     3601 agataaaatt tccacacgac ttcgctgaaa ttcctgttct gacacttgag ccagatggtc
     3661 tcgcatctgt tgtaactggg caaggtaaat agccttttgc tggtcttcca gttcattttt
     3721 ataaaccacc tcaatcaagt ctggcaattc agtcagaact tcttctttct tgcgtcgcat
     3781 cacgaaaggc ttgataaact gagccactcg ctctgctggc aatttcataa attctttctt
     3841 gcttggcaaa agtccgggca tgacgatttg gaaaatagac cacaactcac ccagatggtt
     3901 ttcaatcgga gttcctgaca aggcaaagac cgacggcacc acaaattgtc tcaaggtctg
     3961 ggcaatcttg gtctgggcat ttttcatgac ctgagcctca tctaagaaaa ggaagtcaaa
     4021 ggccatcccc tgataaaact cactgtcctg acggaaggtg gcatagctag tcacatagat
     4081 ttgatggctc tcggcaagaa tctcctcacg acttgctttc aaaccatgaa caacagccac
     4141 atccaactgt ggggcaaatt tctgaaactc atctgcccag ttgtaaatca aacctgacgg
     4201 agcgagaatc aaaacccgac tttcttttgt cacttgacta gtcaaaaaag caatggtctg
     4261 aagggttttc ccaagtccca tatcatcagc caaaatccca ccaaaaccat aatgatggag
     4321 catctgcaac cagccaattc ccttttcctg ataatctcgc aagtcagcct gaacctgagt
     4381 tgcctgtcga ggaaagtcct ctggatgtgt caaatcctgg gccagattct ggaattcttg
     4441 tgaaaaagaa acacggtctc gcccttcaaa gagatgagct aaactgtagg ccaaggattt
     4501 ccgagcctgc aaggtcccat cttttaattc aaattgcccc agttcctgta gattttggcg
     4561 aattttcttg gtttcttcat cgaaaaagta aacttgatta gacgaatcaa tataaaaatc
     4621 ctgattggca accaaggcct gcatggcttg gtcgatttcc tcctgggcaa tatcttgaaa
     4681 atcaaactgg atttccaaga gacctccctt ggaggcaatc tgcacctgag gactcgctag
     4741 gctataaagc tcttctagtt tatctgatag gtcaacatgc ccgagttttt caaagactgg
     4801 aatgatatca tgaaaaaaat gatagacaga ctccgctttt aaggcctgac gccaagattg
     4861 aaaatcggct tcaaagcccg cagccaaaca gacttggaaa attctttctt ctaagtccgc
     4921 gtcacttgaa aatggtaatt cttctagctc ttgtcggcta gatacctgtc tatttccata
     4981 atcaaactga atttctaaac gaatccgatt atcttcttcc ctgtcaaagt aaaaagaggg
     5041 cgcaaaagtt ttgatttgta gacgttctgg agctgaaacg gtgcccatct ggataaaaag
     5101 agtcagacag gaggccaatt tgtctcgatc actgctatca aattgcaggt atttctttcc
     5161 ttgttgaccc acaggtaacg ctttaatttc cttgagaaga cgcatctgct ggtctgttaa
     5221 aaaataaacc tgacctttat ggaaaagtac tgctccctga taaaagacat tgaccctagg
     5281 actctcactg atttccattt caaaatagtc cgagtattct gttactgcaa aggcaaatag
     5341 attggcatca gcatgcatat cctgaaaaag cagggtttgg tagctatcca cttgatggtc
     5401 aaattgaaaa tggggcaagg ccatcagtaa attcacaccc tgctcaaaaa aggtcagagg
     5461 gaaaaagagg tgccgacctt ggttttggaa aaagaggtct gaaaccagcc cttcctccgt
     5521 tagtccgtgc aagaaagtca aaagttcttg gctggcatca tcaaaggctt cccaagaaag
     5581 agactcctca taaatcttgc caatcatata cgactttctc tgctcgacaa tccttaaaaa
     5641 aagtggaata tcccgaatga catagtattt ttggctattg atttggccga ttctcagagt
     5701 ccacaagata tgattggttc ctgcttccac ctgacccaca gctgataact cataggcgca
     5761 ttctgatttt ggagataaaa ttcgatccaa aaacttgcca cccaaggtca ccttggtttc
     5821 aacagcctct ttttcttcat gaccttcttc cagactccac aagatttcct gaccacgctc
     5881 atcatttttc agaaaatgct ctagcgctgc caaatgcaca cagtaacccc tcttttgaaa
     5941 aaaatcacag gcacaaaaaa ccaaatcatc ctctaaacta tagcgcagtt cttcttctgc
     6001 aacgcgagcg tagagccgat tgttcttttc cttgatgata tcaaccttac cagtttcata
     6061 aagggcaaca ccttcgatac gaattttccc cggaatcaat ttagccatat tttcaccttt
     6121 accttatctt tttattatac catattttcg cctatgaaaa tagccttcta ggaagacttt
     6181 tctcctagaa ggctggattt ttaacgtt
//
LOCUS       DAVMHG010000120         6208 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMHG010000120 DAVMHG010000000
VERSION     DAVMHG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.122"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(105..1061)
                     /locus_tag="VYR08_001748"
     CDS             complement(105..1061)
                     /locus_tag="VYR08_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV3467237.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLEGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1061..1396)
                     /locus_tag="VYR08_001749"
     CDS             complement(1061..1396)
                     /locus_tag="VYR08_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV3467238.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(1400..1816)
                     /locus_tag="VYR08_001750"
     CDS             complement(1400..1816)
                     /locus_tag="VYR08_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV3467239.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(1826..2176)
                     /locus_tag="VYR08_001751"
     CDS             complement(1826..2176)
                     /locus_tag="VYR08_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467240.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(2179..2382)
                     /locus_tag="VYR08_001752"
     CDS             complement(2179..2382)
                     /locus_tag="VYR08_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467241.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2363..3744)
                     /locus_tag="VYR08_001753"
                     /pseudo
     CDS             complement(2363..3744)
                     /locus_tag="VYR08_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         1582 a         1444 c         1140 g         2042 t
ORIGIN      
        1 tccctattct tcaagaaaac gcttttttga agaatcagag caaacaaaaa aaccgcaagc
       61 ctgagcctgc ggtgaaagaa caatttagaa agtttccttt ctatttattt aactgtaatc
      121 aagccatctg gctctactgt gaactctggc ttgtctgcca gtgttccgtc tggtttgagg
      181 tagtaccagc ctgttccgtc tgcggactgg acgaaggcgt ttgataccat agcgccttct
      241 ttagcgtcta agtagtacca tgtgtccttg tacttgaccc agcctgtctt catggcacct
      301 tcttcgttga aatagtacca cttctcagca attttcttcc agcctgtagc catttcgcct
      361 gagttgtcaa accagtacca gttgccgtct gtgtacttct tccagcggtc tgcaagcata
      421 tagcctgagc catcgaaata ataccaggta ccgttgattt tctcaaactt atcttttgga
      481 taagagccgt ctgagtgtac gtaccagtag ccagtgccat ttttctgcca gcctgttgca
      541 gcgctcaagc cgttttcgat gtcttgctta aactgctcac ggctaatgcc ccaacttgca
      601 agatatggat atggatccac atggtctgag tggttgtttg gttggttatt ggtacagtat
      661 tcatgcgtct tgataccttc caagtcgtct gtatcaagag tcttcggcaa acctgcttca
      721 tctgctagat tgcgtaagag ttcgatataa aggcggtagt ccgtcatgaa ctcttcttta
      781 gttgaatggc tttcaatcag ttcaaccgct gcgtaactct cagtattcca accgccccca
      841 acatcccaac ttccgttgtt cacaggacct acttgcatga cacggccatt tccgacaaca
      901 tgtgaaaaga accctagttc agggtccttt ctatagtggt aatcagcctc attttgagct
      961 gttgagttac ggttgcctgt tgagtgggcg tgtacttgtc gataaggctg caccccaacc
     1021 tgtggcaagc ctgttcgtag tctgtttcta tcgatatcca ttactcttgt cctttccaag
     1081 cgtcgttcat ctgcttcacg gctgactcca caaaggtgtc taagtccttg tccgtcatac
     1141 tgatattgta ttttgtaagc tcagcacgga ctttagcacg agcctgtgcc agtttttcat
     1201 ctcccttgta gccagtttca gcagctacct gctccacggc atttactgca tttttggcca
     1261 agatttcaac gattttgatg gtcttttctc cacctttttg aaccaggtag tccttgactg
     1321 ccctaactac aacccctgcc aaaacgacta ggatgctgat tgctccatta gtaatgattt
     1381 cagtaatttg ttgcatttgt tattctcctt ttttcgtatc atcatctttt tcaagtaatc
     1441 gctgaaatac ttttacaatc ggctgaaaaa gagtaacatt tccttttaat ttgcggtaat
     1501 tttcaatgag agattgaaaa gtaaatgcga tgtacccgag atagattgag tacaagaatg
     1561 caaaacctgt cttttcaggc aacaaaacgg acgccggaat gaggatcatc agtaagagaa
     1621 cccctgaaat cttacgaagg agcccgttaa tgccgatttt gctcttgtac tcgatgtcag
     1681 gattgataat cgccgcaatc gtccctgtca caaaatcaat gatttccatt gagacaatca
     1741 gtgctagagc gtacaagacc aaaccatctt cagtctgtac gacacttctt agaaaattga
     1801 aaaattcaat ttgcatacac acctcctatt ctttaggttt taccgttgga tccgtccagt
     1861 caggattgcc ctctgcatca aatttcatga tatagaattc atgattcaac agaacggcta
     1921 cgttgattgt tgcgattgta ccaccccact gattgaacgc ccaaacggtt tcaacatcct
     1981 tgaattggcg acggccattt acaatcacag gacgtttttg aacgtcacga tacatataga
     2041 agtcatcgct tacattcttg caacgaatga actctccatt ttctttcatg tagcgcaaag
     2101 cactcgcaag atcaaatggt tctgtgattt ttgtaaggtc tagtaagtta tctgtgtttt
     2161 gaattgtttc tgccatgtct attctccttt gtctgctggt ttagtttgtt catcaagcag
     2221 agcttccagc tcatccactc gtgcttgaag tctttgattt tcttcccttt gctcatccaa
     2281 ctgaatactc aagatattac ttgtaatcat cgaatttgtt gaagttgttg atatttcact
     2341 aattgtcatt cgtaaggctt ggttaagctg ttctgtgttc attttctaag ttctccaatc
     2401 tgtgtgttcg ttttctattt tcaagagcaa gctcctaaat tgctttaagt gcgatattgg
     2461 tcaacatgtt gttattcatg ctattgtttt ctccattttt tctatttttt gatttaattc
     2521 ttgaatagcc ttgattaagt aaggaactaa agcggtatag tcgatatgta gatagccatc
     2581 tggattctca ggatctcgtg agacaattct tggaacgatg gtttcagcct cttgagctat
     2641 tagaccaatc tcctcatgtt tcttattttc gatgaaatca aatgcaacca ttcttaatct
     2701 gttgatttta tccaaggctt tcacagctgt atctgtgatg ttctctttta agcgtctatc
     2761 tgatttttgt tccatccaat acttcaagct accgctaccg acctgattcc accaaacaac
     2821 cgcattcctt ccgcctttgg gattccaacc atcaccaagc acatctttac ttccaagttc
     2881 gataccattt gaaaacacag gagaacgaga aaaagtagta ttcccataga agtttgctct
     2941 cgatgaattc gaaaaatcca cttgatcata aaaaccgact tcattcctac agtacatttt
     3001 cccatcagta ttgacgttcc atgctttagg tccggcatag ttccaattat ttccccagtt
     3061 cgcccagaag gctgtccgga ctccataccc ggcaccattc cccataccaa cagagaactg
     3121 attgacacct gaaatccagc gaccgccacc ctggtcaaat tgaccaagtg tgaatccacc
     3181 aattcggcct tgataggctt ctaggaaggt tgagctagaa atgacggact caaccttagt
     3241 agagaagata cgtttagata tcagttggtc aataaaagca tcagttgcag ttaatttttt
     3301 aataagcgca ttgtcaactt tcaacttctc agcagttacc gcttcagctt ctaatatcgt
     3361 agtcgtgacc gaaccagctt caaaattggc cgttttgagc ttatcaacca tggcagactt
     3421 gatgactgct ctgtcaatca gggtctctcc agtgatgtgg gtcaatttcc caactaagcg
     3481 gttatgtcca ttggcgccaa gattgattcc agagatgata tctccagccg agttgatgtt
     3541 ttcaactacc catgagccag ctagttgagt catttttgtt tgcgttgctt caagcttctt
     3601 attcgcatct gcgactgcat cttctggatg tggttgccat gttctaggtt tataaccttt
     3661 gtacaagtca acttctgtaa tatacaaatc agctgttcct gatgatgagc cattgttatc
     3721 aaaacgaatg taagcattat ccatttctcc ggaattaaaa gttactgaga catcttcgca
     3781 tctagaggta gatagtttct tgctgctaac aactttctta acgattgtga atccatcgct
     3841 ctcgcctgct cttcgtccca aaatataaac atcatagctt gcgagagcac tgttgttaaa
     3901 tcctctaaaa ttcagtacat agtcagtatt tcgttcaaga ttaaaacggt gactatacaa
     3961 aaagttttcg tttttagttg cattacttaa acgcataagg tctttctgcc cgttgtgata
     4021 aaagctatgc ttaaccaatc ttcctaaatt ttgagttgag ccccattcat tcgtagcatt
     4081 tttaaaatca ctattcttaa tgaggttagg gccgcttaca ctatatttcc caacctcaac
     4141 ctgaaacagt tgattagtca gagccatgcg agcaacctta tccgcaattc cattttcagt
     4201 attgcccaaa atccgctcgt aaagtttact ggtttcctta acacgctgga agtcagtagt
     4261 ctctactttt cgcgctagtt gattggtcac attcgcaaat tgactatcag cattcgcttt
     4321 gtttgcagaa acctgatcag atattctacc catttgtcgt tcagcattat ccttgtttgt
     4381 agcgacctga gtctttaaat ttgaaatctg attatctgtg ccttgtttat tactgtttat
     4441 ccgatttgaa agatttgaaa tctgagtagt ggttccttgc tcactgcttg taagtctatt
     4501 tgatagacca ctgatttgac cgcccacatc ttgcttataa gtagttatct gacttgaaat
     4561 atccgtgaac ttaccatcta cagattgacg atagctagcg atttgactag cgatgtcttt
     4621 attcgcacta gttttaacag cttcaatcct ctgattgata cccttaacat cttcttgata
     4681 agtagcctta ccaacgaaat cacgattgac cagctcacgg actgctgtcg cttgtctcgc
     4741 gctctcctca cgagtatagc gctgtagggc ttcctgtcgc tgaccgtctt tatttacata
     4801 ttcctgaata gctgataagt cggttcgcaa gccctgagct gtccgctcaa aggtagcctt
     4861 agcctcagtg atgagaccat cagcgtcctc aggcgcagga ctccagtccg tcgccacact
     4921 accgatttca accttgattc ctgttaccca agctgtaccg cttgtagcac cttcaagatt
     4981 gaatcgcaat gatgtcttca attgatcaaa atttgttttt tcagagtagt cataagtgaa
     5041 tgtaatatat ttccaatctg ccgaaccttt atacatacca agcgtagcat aatctggacc
     5101 actctgtact ccggtctcac tattttttct aaaaagataa tgtttgaagc aattaaatac
     5161 attccaaaaa tttcgacctt ggactacatt ttcgtacttg atccaagcgc taaaagtaac
     5221 tttttggtac aaccttgagc tgaaatctgg ttcaaggttg aacgttaaag tagagttgtt
     5281 ctctagccta tagcattctt tttgacctgt gacgtggttt tcaggtaatt tttcaattac
     5341 agctccaacc gtcttggatt ttatccatag attccggcct cccaccttca tttttgaaaa
     5401 ttcttcacgc aatttcccgg cttcagatac aactaaagtc ttatctgctt tatccttggt
     5461 tgcgttcagg atttcctgac ggatagagcc agctcgcacc tcaaattcag cctgactcaa
     5521 cttctgattt agcttgttct gcgtgtctgt ctcaagactc ttcaccgact gcctaatatt
     5581 ctcagcagtc acgttgagtg cgctgatatc cactttagtt ctaaggcctt cagtcagacg
     5641 gcttacacca gcgtcgagtg agttggctcg ttgtttgaag gtcgattcaa ctgttgaaat
     5701 ctgaccttct atatcttcag gagcttcgct gtaatcagtc gctaacgttc ctgattcgat
     5761 tttcggagcg catatttcga taattcctgc gccagattgt ccaaattgaa ttgaattttc
     5821 aattgcatca gcagtaaatg taaatgaata tttttgccaa tttttatgag agatggattt
     5881 ctgaaatttg cgatttaaat cggtattatt tgcccacgaa cgaaatagca agttcacgtt
     5941 tgcatttggg ctatcgctag caatccttgc ataacaagaa atcgtgtact tttcaccaat
     6001 tcgcaatcta acaagttgag ttaaatcttt atttccacca ttcgtatttt caataacacg
     6061 aatcatgttt tttatcattt ttttgggagg atccaaaact tctacgctaa tcgctcgccc
     6121 atttcctccg cttgcactca tccaacatcc ttttgaacga tcgccaatca acaaactcgc
     6181 agtattacgc aagaggttga ttcctccg
//
LOCUS       DAVMHG010000121         6143 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMHG010000121 DAVMHG010000000
VERSION     DAVMHG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.123"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(28..1986)
                     /gene="ligA"
                     /locus_tag="VYR08_001754"
     CDS             complement(28..1986)
                     /gene="ligA"
                     /locus_tag="VYR08_001754"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV3467242.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLNYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(2079..3257)
                     /locus_tag="VYR08_001755"
     CDS             complement(2079..3257)
                     /locus_tag="VYR08_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3467243.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSIIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(3337..4200)
                     /locus_tag="VYR08_001756"
     CDS             complement(3337..4200)
                     /locus_tag="VYR08_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3467244.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(4203..6104)
                     /locus_tag="VYR08_001757"
     CDS             complement(4203..6104)
                     /locus_tag="VYR08_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV3467245.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKFSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         1863 a         1397 c         1096 g         1787 t
ORIGIN      
        1 ctctaaactc tgtttttaaa cgatccatta caaactttct agccatgcct catctctgac
       61 ctggatacca agttcttgtg ctttttgcag tttacttcca gcgtctgcac ctaccacgac
      121 gaggtcggtc tttttagaaa cactacctgt cactttggca cccagacttt cgagtttact
      181 tttagcttct gagcgcttga gtcgttccaa ttttcctgtc aataccacgg tcaaacctga
      241 caaggccgca tccgctacta ccgtctgtcc tttatagttc agattgaccc cagtttcttt
      301 caattctctg agcagaattt cagagccttc tgtcgcaaaa taagtctgaa gacttttggc
      361 aatcacgcca cctagacttt caatactagc cacttcctct gaatctgcct gatacagatt
      421 ttcaattgaa tggaaatatt gaagtaaaag ctgactagcc ttgcttccaa catgacgaat
      481 tcccaaacca aataagagct tctcggcaga attttccttt gatgcttgga tagcctgata
      541 cagtttagca gcggactttt ccttaacccc ctctaaaagg aggaaatcct cttcttgcaa
      601 acgataaata tccgccacat ccttgactaa attagcagca aaaagcttct caacaataga
      661 tggaccaagg cctgtaatat tcatagcatc acgagaagca aagtgaatca agccttccat
      721 gatttgagca gggcaacgcg gattgataca acgtagggcc acttcatctt caaagtgcaa
      781 caagtcagag ttacaacttg gacagtttgt agggatatct agtttttctt cagaaacccg
      841 tttggactct accacacgta aaacggcagg gatgatgtca ccagccttat atacaatgac
      901 cgtatcgtct tttcggatat ctttttcagc aatataatct acattgtgca gggtcgcacg
      961 gctaacagtc gtaccggcaa gttgtactgg tgttagatta gcagttggag ttacaacacc
     1021 ggtacggcca actgtccagt caactgataa gagttgagct tctttttctt cagcagggaa
     1081 cttgtaggct actgcccact ttggagcctt aacggtaaaa ccaagttctt cttgacttgc
     1141 taggtcgttg accttgatta ccactccatc aatatcgtaa ggcagatttt cccgttcctg
     1201 tcctacttct tggataaaat tccagatttc atctatgttt tcagccaaga ttcgcttagg
     1261 attgaccaca aaacctagtt gttctaggta cttcaaaccc ttttcttggc tatcacgagt
     1321 tgaagggctg gcttcttgat agagaaacgt tgcaagatta cgcttggcaa ctactgctgt
     1381 atccaactga cgcagagttc ctgctgccgc attacgagga ttagcaaatt caggctctcc
     1441 attttcttgg cgcacttggt taacttggtc aaaggaagcg cgtggcatgt aacattcccc
     1501 acgaactgtg atatctagtt cttctggcaa agtcaaaggg atgtccttaa cacgcttgag
     1561 gttttctgtg atattttcac caattgaacc atctccacgt gttaccccag caaccaaaat
     1621 ccccttttca taagtcagcg agatagataa gccatcgatt ttcagctcac aaatataggt
     1681 cggatgagcc acttccttac gaacacgcgc atcaaaagca tctagctcct cacgtgaaaa
     1741 agcatcctgc aaactataaa gaggatactg atgactgtat ttttcaaaac catctaaaac
     1801 cttgccacca acacgatgag tcggactgtc tgctagcact tgctctggat aagcagtttc
     1861 taactcgacc aactcacggt aaaggcggtc atactcactg tctgaaaccg agggattatc
     1921 gctggtatag tactcagtcg catagcgatt gagcaaagcg actaactcat tcattctttt
     1981 attcataaga ccattttacc ataaaacaag ctctcctcac aaacgagaag ggcggaaaaa
     2041 acacttagtt tgaaattatt tttgaaactc aagcaacctt atatcaattt ttcaaaatga
     2101 gttcgaacat aaataaacga tatacaagac aagatgataa caccacttcc aattatcagg
     2161 aaagaagaga gatgtacact tggcaagact gtcataaatc cttttgcaat aggcataaat
     2221 agaatagcta aggtaaaaat tatactcagt actcttccaa gaaattcgct ctcaaccttg
     2281 gtttgtactt gagtaaaaaa gtgaatatta aaaatcgtca taaacaattc acaaactaaa
     2341 tttccagaaa aggaaagaaa agttggaagt ggtaatccca tcataaaaac tccgacacct
     2401 gtcaaagcca gtaaaatcaa aagattataa atattagctt taattttact agctagaaga
     2461 gccccaatga tggaaccaat agcccccata gttaaaatac ttgcataggc tccttctgac
     2521 ccgtaaagct gattcgaaaa gggaagtaga aattcaaaag ctgcaaaaaa gaaattaacg
     2581 ctggaagcta ccagcaaaag gaagaaaatt tcttgctgat gccagatata gtgtaaccca
     2641 tccttgatat ctacaaaaat atctctccca gtaaaagcct ttttctcttg aacttttgct
     2701 tcctcttttg gaaggaaagc cactagaaca aaagcaatga aaaaagtcag cgagtctagc
     2761 agtagcgtca tatggagact tgcaaactgt aaaacaagga aggaaagaac aggagagcta
     2821 acacctacaa cctgcaaaac cagctctaag cgagaattat agatcacaat ctcatctttc
     2881 tccaccactt cagttatgat agctttattg gctgtgcgag aaaaggcaaa agcaatagcc
     2941 tgcacaatgt tagcaacaat caaagcgcca atcatccagc tatcattcct tatgaaagaa
     3001 atagccagac aaagaatccc acaaacaaga tctgccgtca ttaaaatctt acgacgagaa
     3061 aaacggtctg aaataactcc gccaaaggga ttgacgagaa tagatgtgac gagctcagaa
     3121 atctgataca ttcctaaaac tgtctgtcct atagtcccca tagaagccaa ccagacacta
     3181 tttccataat catagagcat atttcccatt ttattgatag ccccacggct aatcaactgc
     3241 actgcatagc gattcatatt aaagctcctc tcaaattttg aaactattgt atcaaaaccg
     3301 aaaggagctt tttatttttt cccttatttg ggaaaattaa cttttgacaa atttttcgta
     3361 gtgttcctga taataggcta cttgctctgg aagacctaac acatcaaaaa tatgcatggc
     3421 ctcttgcatc tgcttacagc cttctttaca ctgtcctttt tgatataagg caaaaccttt
     3481 taaataatgg aaaacattac gctcataaag cttaatacct ttgtcaataa tcttctctgt
     3541 ataagcctca aaatagttgg cattataaaa agaagaatgc tctaaacaat gctggtaaca
     3601 attgagggcc aaaattaaca ctaatctctt atggcgacta atctcttggt aaaattcctc
     3661 cctctccata acttctctac caatccgagt gacatagtct acatcgtaga aactatagag
     3721 gttaccgaaa agaatcaact catacatggt ccattcttct gttttgaaga gataatctgc
     3781 taccttatcc aaatcatcct gcttcatatc ataactcgca tctctttgac aaatcagacc
     3841 ttgtagcaaa atccagttca gctcaaaata aaggggagtc gtcgaactct tagacttttc
     3901 aagttgttct ctttgaagct tttgaaaacc tgcaatatcg tttgaatagt aaagtgggat
     3961 aatctgtgcc atcatagaca catgttcatg attatgaaaa ttccttgcct tatccatgaa
     4021 attttcgatt gttacatgaa tgttatccaa aatctcaaag aaacgggaga ctgccaggtc
     4081 agactcccca agctcaaagc gagataactg agaggtagag caggattcgc ctgctgcttc
     4141 ctttaaagaa taatttccac ttgttcgaaa ttcacgaaat acttttccaa gatgttccat
     4201 ctttacacct gctctgataa ttcttcccac tcaagcatag cttcttcctg acgatggctg
     4261 attttgtcca gctcagcctg taattccatg agtttgtcgg catcgtttgt ttccaacatt
     4321 tgttcagaaa tggcttggct ttgactttct agctcttcaa tttcagcttc tagactttcg
     4381 atttgtcgca tgagtttgcg aacttctttt tgactttctt tctgggcctg atagtcattg
     4441 actggacttg cttcctttgc ttgattgcta gttgaagctt cctcagtctg actcatttct
     4501 gctgttgctt tcttctcaac atagtagtcg taatctccaa ggtagagagt tgaaccattc
     4561 tcagacaatt ccaaaacatg agttgccaca cgattgataa agtaacgatc atgactgaca
     4621 aaaagcaagg taccatcaaa gtcaatcaag gcattttcta gcacttcctt actatcaata
     4681 tccaagtgat tggtcggctc atccagaatc aaaaagttat tgttttccat agaaaattta
     4741 gctaaaagca aacgagcttt ttcgccacca gatagcatgc cgactgattt tttaacatca
     4801 tctcctgaga aaaggaaggc tccaagacgg ttgcggattt caacttctgg tgtcagtttg
     4861 aaatcattcc agagttcatc cagcaccgta ttacttggtg tcagcttgct ttgggtttgg
     4921 tcatagtaac caacctcaac attagcgcca aagcgctttt ctcccttgat aaaaggaatc
     4981 tggtccacaa tagacttgat aaaggttgac ttgccgatac catttggacc aacgatagcg
     5041 acagcattca tcttacgaag atctaggttg ataggttgtg acaagacttc cccgtcataa
     5101 ccaatagcag tattttcaac agtcaaaaca acattgcccg acgttttttc agactggaag
     5161 gtcatgttgg ctgctttctt gccagcttca ggcttgtcca aacgctccat tttttctagt
     5221 tgtttacggc gagattgagc acgtttagtc gttgaagcac gaactagatt gcgattgaca
     5281 aagtcttcca gagcagcgat ttccttctgt tgcttttcat agttttttgc ctcagtagct
     5341 agcttttgct ccttcaattc gacaaaacga gagtaattcc ccacatagcg atccaaggaa
     5401 tgcttggtca aatctagcgt aattgtcgca accttgtcca agaaataacg gtcgtggctg
     5461 acgataatga gggcaccgct atagtttacc aagtaattct ctagccaggc gatggtttca
     5521 atatccaagt ggttagttgg ctcgtccaag accaagagat tgggcttttc aaggagcatt
     5581 ttggcaagtg ccaaacgagt attttgacca ccagaaagct cagcaatttt catctgccac
     5641 atagactcgt caaacttgaa tccattcaaa atcgctcgaa tatcagcttc ataggtaaag
     5701 ccgcctgctt ggcgaaaatt ctcagataag tggtcgtaat ctgacatcag tttatccaat
     5761 tcatcgccag acttttcacc catctccaac tccatctgac gcagttgtct ctccgtccga
     5821 cgcaaattat taaagacatg aagcatttca tcgtagatgg tattttcaga ctcaaaacgg
     5881 ctatcttggg ctaggtaaga cagagaaata tcttttttct tattgatttc tccgctagtt
     5941 ggctcctctt ctccaactaa aatcttcaaa agagtagact tacctgcacc atttttccca
     6001 acaagagcaa tccgatctcg ttcatcaacc tgcaggttga tattatcgaa aagaacctct
     6061 cctgcaaaag aacgttcaat tttattagct tgtaaaataa tcataagagt agtatagcat
     6121 gtttccctaa ggcattcaag ata
//
LOCUS       DAVMHG010000122         6106 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMHG010000122 DAVMHG010000000
VERSION     DAVMHG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.124"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            316..888
                     /locus_tag="VYR08_001758"
     CDS             316..888
                     /locus_tag="VYR08_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467246.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGIYLAQLWDKNDAIPNNGRINF"
     gene            complement(1933..4191)
                     /locus_tag="VYR08_001759"
     CDS             complement(1933..4191)
                     /locus_tag="VYR08_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEV3467247.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(4217..5734)
                     /gene="malQ"
                     /locus_tag="VYR08_001760"
     CDS             complement(4217..5734)
                     /gene="malQ"
                     /locus_tag="VYR08_001760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV3467248.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
BASE COUNT         1901 a         1183 c         1172 g         1850 t
ORIGIN      
        1 atcttcctcc aaaatcaata ttaaaagtag ccagtcaatc atagttctaa tctttatttt
       61 cgattcgaaa ttacaaactt atcaatgaac aataagtgat ataaggccag taccagaccc
      121 caaacgctac cacgaaaaga gaattcttga tgattgagag taaacaaatc atggttacca
      181 atatagaggc aggtcaaaat aacaaataaa atccaaaata aatccgtcca tttatatttt
      241 gaaaaatatt tttttgacta taataacagt atatgataaa atgtaagcag atacaaataa
      301 aaaggagaaa agctcatgaa caaattaatg aaatttattt cggttttttt gacgtcaatt
      361 gtgttaattg tatcagcgat tccaagtgtt tcagctgtat acgcttctga acaagtatca
      421 caaattgaaa caaatatgga acttcaacct gtcacttctc taacagaaga acaaatcaat
      481 acacttgcaa acgaaatcca atcttttcat ccagacgtct cacaacaatg gatcaaagaa
      541 gtaattaacc gacaattaca aggcgattat acaatcccac ctacatactc tccatttaga
      601 gcagcttggc aaggtattac agttaatcaa atgggtgctc tattagatac tgcaatagct
      661 ttagcattag gaggaactac tgcaggcctt gcaaatctaa ttaaagtaaa aggaaaacat
      721 gcagcaaaaa gtgctattcg ttcagcaatt tctagatatc taggtagttg gtttgtaaat
      781 gatgttgctt tagaattcgc tatgaattta ttatcaccgg ggatttattt agcacaatta
      841 tgggataaaa atgatgccat tcctaacaac ggaaggatta acttttaatc atgagatata
      901 caataaaatt aattgggtat aacgtatttt cgttgtttat attctttatc ttatcaatgt
      961 tgtttaaagt tccctttagg tggtggatca gcattgcaat gacgacttcg tcgtacattg
     1021 tatataatta cttaaagaaa aagagagaaa aggaatgatc ttataccaga tcattccttt
     1081 tctgctttct gtaaatccct cggagtgtgt ttggcgcttt gatacgaagt gtcaaagctg
     1141 tataaacgtt actatttcca aaaaagtaac agaaaattcc tgctttgtag gactgctttg
     1201 ggtgagtaat agacatgtag actcacccta tctcctgatg gcgaaaagaa tttagcaatg
     1261 atgcaagttg ttctgaactc gagaaaaaac atgccgaaat cgaagaaaag cgtgcagaac
     1321 ttgaagcgaa gaagcaagaa atgttgaagc tcttggataa atagaattta gaaaacagaa
     1381 aaaactaaaa aaaatgtagg ggttatttcc ctacattctt aaaataactt agtacataaa
     1441 tgacaatgct gccactgccg tcaatattgc aaaaactatt ttccaaattt ttttattttt
     1501 tccaaaaatc ttatcaggta acaagcttat tacaagaaag gtaataaaca acaccaattt
     1561 aagtgtgtat atcataaaaa caccttcttt cctacaatta tattataata ctttatgtaa
     1621 gcgaatacaa tagatgtgag gtaaccaact acgaaaaaat ataaggctga agacagtaaa
     1681 aaccagcatt taaagccttc actgtatcta ggatttccct atttttagac aagattgacc
     1741 caaatccaca gcatttaaaa aagcgaattt caattgaaac tcgctttttt acaagatgta
     1801 ttagtataaa tttgtatctt atactcaatg aaaatcaaag agcaaactag gaagctagcc
     1861 gcaggttgct caaagcaccg ctttgaggtt gcagataaag ctgacgtggt ttgaagagat
     1921 tttcgaagag tattagttca agtgccagat gtcttcgtta tactgagcga ttgtacggtc
     1981 agatgagaag aatcctgctt tagaaatgtt aacgatgact ttatccaacc atgcgtcacg
     2041 gtcttcgtag tcagcaagca tttgctcttt gactttgatg tagtcttcca aatcaagaag
     2101 agtcatgaac cagtctttgt tgatcaattc attgtaaaga cgttccaagc gctctttgtt
     2161 tccagctgca agaactgcat cactaacgat gaagtcaacc aatggtttga tagcttcacg
     2221 agcgtagaat tcgcttgatt tgtaagctgc ttttgcgtaa aggtcgataa cagtttctga
     2281 atcttcaccg aagatgtaga tgttttcttc tccaaccaac tcagcgattt ccacgttagc
     2341 accgtccata gtaccaagtg tcaaagctcc gttcaacatg aatttcatgt taccagtacc
     2401 tgaagcttct ttagaagcaa gtgagatttg ttctgagata tcacatgctg ggataaggaa
     2461 acttgctgca gtaacgttgt agttttcaac cataactact tgcaagtgtg gagctactgc
     2521 tggatcgtta gcaataactt ctgacatgca aaggattaaa tggataatgt cttgagcgat
     2581 tgtgtaggct ggagctgctt taccaccaaa gaagattgtg attggacgag cagggatgtt
     2641 accagctttg atgtcaaggt atttgtggat cacgtacaaa gcgttcattt gttggcgttt
     2701 gtactcgtga agacgtttga tttggatatc aaagatagaa tttggattga tttccacacc
     2761 ttggtgttct ttcaagtgac gagccaattt acgtttgttg tgagccttga tgctttccaa
     2821 tttttctttg acagctgctt tgtcttcata agacaacagt ttttcaagct catctgcttc
     2881 atggtgccaa ccatctccaa gaatctcatc caagtagtga gacaatcttg ggttagcatg
     2941 cataagccaa cgacggaaag tgataccgtt tgttttgttg ttgaactttt ctgggtaaag
     3001 gtcgtagaag gctttcaact cagaattttt caagatttca gtatggagtg ctgcaacccc
     3061 gttaacactg tatccgtagt ggatatccat gtgagccatg tgaacacgtc cgctctcatc
     3121 gatgatttga acagctggat ctttgtactc tgccttcaca cgacggtcca attcttcgat
     3181 gattggtacc aagtgaggaa ccacttcttg caagaattca agaggccatt tttcaagcgc
     3241 ttcagcaagg attgtgtggt tagtgtaggc agtcatgcta cgaacaattg agattgcttc
     3301 gtcaagatcg ataccacgtg cagtcaaaag acgaatcaat tcaggaatca ccattgatgg
     3361 gtgagtatcg ttgatttgga caactgcgta gtcagcaagg tcatgcaagt tgcttccttt
     3421 ttcgattgct tcgtcgatga tcaattgcgc accgtttgaa accatgaagt attgttggaa
     3481 gatacggagc aattcacctt gacggtcact atcatctggg taaaggaaga gagttaagtt
     3541 gcgagcgata tctgtcttgt caaagttgat accatcttta ataatagaag aatcaactga
     3601 atccaagtca aacaaacgca agcggttttt agtcgctgtt tcataaccag taacatcaat
     3661 atcgtaaaga gttgatgtca aagtaaagtc tgcaaatggt acttggtagc tacggcttga
     3721 gcgaaccaac cagttttgct ctgtcaacca tgcatttgga attgtttctt gttggttgtt
     3781 tttaagaact tgttggaaaa gaccaaagtg gtagttaaga ccaacaccgt caccattcaa
     3841 accaagagta gcaattgagt cgataaagca ggcagccaaa cgtcccaaac caccattacc
     3901 aagagatggt tccaattcaa cttcttcaac ttcgatcaag tctttacctg cagctgcaag
     3961 ttctttttta acatcgtcgt aaagaccaag gttaatcaag ttgtttgaca agagtttacc
     4021 aatcaagaac tcagctgaga tgtagtaaac ttttttctta ccagtgttga ctggtttttg
     4081 gctgcttgca agcttgctgt agttaagaag agcaaggtaa agctcttcat tgctacattc
     4141 tgcaatggtt ttattgtaac gattttgtac aaattcttgt agtgataaca tgtttagttg
     4201 tctcctgatt attgtcttat ttctttaaat ctaccaaatt ttcattaatt cggcgataaa
     4261 ttgttgtcaa gtcaagcaaa ccttcctcga cagctggtgt caattgatct tcagtcatac
     4321 gccaagacca gtttccacca agggtagatg ggaagttcat acgagctgcc tcatccaatt
     4381 ctagtaaatc ttgcatagtt gcaattgcca taaagctaac tgatgaaaat actgtacgaa
     4441 gcatagcgtg taccactgtt tcgtattctt tacggttcgt gtaacgagcc atgtactcac
     4501 gagtcgcatc atcaatctca ttacggtacc aaccaagaac cgtattgtta tcgtgtgttc
     4561 ctgtgtacat aactgagtta gcaggtgcca agtgtgggct atcaatgctt tcgtcttctg
     4621 ggttgaaggc aaattgaaga atcttcattc ctgggaagcc agtacgttca cgcaattcga
     4681 tcacttcatc tgtcatgaag ccaaggtctt ctgcgatgat gtttagctca ccaagttctt
     4741 ccttaacggc tgcaaaaagc ttgtagcctg gacctttcac ccactcacca ggtgctgctg
     4801 tatcggaacc agcagggatt tcccaataag attcgaagcc acggaagtgg tcgatacgaa
     4861 cgatatcgta gattttgaag ctttcacgca agcgttcaat ccaccatttg tagccgtctt
     4921 tgtccattgc ttcccagtca tagattgggt taccccaaag ctgaccagtt acagaaaact
     4981 catctggtgg acatcctgcg atacaagtag ccttaccatt gacatctgtt ttgaagagat
     5041 gtggatttgc ccacatatca cttgaatctt ccgctacgta gattggcatg tccccaacga
     5101 tttcgatgtg gttgtcgtta gcgtaagctt tcaatttcaa ccattgttgg aagaagaagt
     5161 attgagtcac acggtggtaa accaacttgt ctgccaattg ctcacgatag ctttcaagtg
     5221 ctgaagcttt acgagcacga gcatctgcat ctggccattc agtccaagca agattgtcaa
     5281 aatgctcttt gatagccata tactcagcaa agagctcaag ccatgattgg ttgtcttgag
     5341 caaatttctc aaaatcttta acatctccga cttcaaagaa acgcttcacc gctttttcta
     5401 aaagaggacg acgtgcatag tagattttag catagtcaac ttcagacgca tcgctaccaa
     5461 agtcaactcc ttcaaggtca cttgcttcca acaaaccttg ctccaccaag atatctaaat
     5521 cgataaaatg agtgtttcct gcgaaggctg agaaagattg gtaaggagaa tccccgtaac
     5581 tagttgctcc taatggaagg atttgccagt aacgttgttt tgtacggacc aagaaatcaa
     5641 cgaagtcgta agcactttga ccaaatgatc cgattccgta agctcctgga agagaagaga
     5701 tgtgcatcaa cacaccactt tgacgttttt tcataatagc acctcgtgtg ttaaaataat
     5761 ggaacgttgc gtatttttca gacgcaaacg tttgcgttac ttataagtat actccttttc
     5821 aagcataatt gcaagcgttt ttaaaaaact tttttgatta tttttaagga gactaactca
     5881 ttttagcaaa gataacttaa aactatctaa tcctaaggtt tgactctcac gagacagtct
     5941 acaaagtaca aaacctcctt agggagcttg aatttatatg taacaaagca caaacgtcta
     6001 tagaaaatag atagggatta gaaacaggga ggtagcccct cctgatttcc ctctttagac
     6061 agattccata tatttttatt aaaaatattg tattcttatc tatttc
//
LOCUS       DAVMHG010000123         5855 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMHG010000123 DAVMHG010000000
VERSION     DAVMHG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.125"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            14..1375
                     /gene="dnaA"
                     /locus_tag="VYR08_001761"
     CDS             14..1375
                     /gene="dnaA"
                     /locus_tag="VYR08_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV3467249.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            1534..2670
                     /gene="dnaN"
                     /locus_tag="VYR08_001762"
     CDS             1534..2670
                     /gene="dnaN"
                     /locus_tag="VYR08_001762"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV3467250.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            2735..2929
                     /locus_tag="VYR08_001763"
     CDS             2735..2929
                     /locus_tag="VYR08_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV3467251.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            3013..4128
                     /gene="ychF"
                     /locus_tag="VYR08_001764"
     CDS             3013..4128
                     /gene="ychF"
                     /locus_tag="VYR08_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV3467252.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPKAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            4199..4768
                     /locus_tag="VYR08_001765"
     CDS             4199..4768
                     /locus_tag="VYR08_001765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV3467253.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            4769..>5855
                     /locus_tag="VYR08_001766"
     CDS             4769..>5855
                     /locus_tag="VYR08_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV3467254.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGN"
BASE COUNT         2016 a          939 c         1127 g         1773 t
ORIGIN      
        1 ggaggagaaa ggattgaaag aaaaacaatt ttggaatcgt atattagaat ttgcacaaga
       61 aagactgact cgatccatgt atgatttcta tgctattcaa gctgaactca tcaaggtaga
      121 ggaaaatgtt gccactatat ttctacctcg ctctgaaatg gaaatggtct gggaaaaaca
      181 actaaaagat attattgtag tagctggttt tgaaatttat gacgctgaaa taactcccca
      241 ctatattttc accaaacctc aagatacgac tagctcacaa gttgaagaag ctacaaattt
      301 aactctttat gactatagtc caaagttagt atctattcct tattcagata cgggattaaa
      361 agaaaagtat acctttgata actttattca aggggatgga aatgtttggg ctgtatcagc
      421 cgctttagct gtctctgaag atttggctct gacctataac cctcttttta tctatggagg
      481 accaggcctt ggtaagactc acttattaaa cgctattgga aatgaaattc taaaaaatat
      541 tcctaatgcg cgtgttaaat atatccctgc cgaaagcttt attaatgact ttcttgatca
      601 cctaagactt ggggaaatgg aaaagtttaa aaagacctat cgtagtcttg atcttttgtt
      661 aatcgatgat atacagtcac tcagcggaaa aaaagtcgca actcaggaag aatttttcaa
      721 tacctttaac gcccttcatg acaagcaaaa acagattgtc ctaacgagtg atcgtagtcc
      781 aaaacatcta gaagggctcg aggagaggct tgtcacgcgt tttagttggg gattgacaca
      841 aactatcaca ccccctgact ttgaaacacg tattgccatt ttacaaagta agacggaaca
      901 tttaggctac aatttccaaa gtgatactct agaataccta gctgggcaat ttgattcaaa
      961 tgttcgagat cttgagggag ccatcaacga catcacttta attgccagag taaaaaaaat
     1021 caaggatatc actattgata ttgctgcaga agccattaga gcccgcaaac aagatgttag
     1081 ccaaatgctc gtcatcccaa ttgataaaat ccaaactgaa gttggtaact tttatggtgt
     1141 tagtatcaaa gaaatgaagg gaagtagacg ccttcaaaat attgttttgg cccgtcaagt
     1201 agccatgtat ttatctagag aactaacaga taatagtctt ccaaaaattg ggaaggaatt
     1261 tgggggaaaa gatcatacca cagtcattca tgcccatgcc aaaataaaat ctttgattga
     1321 tcaagacgat aatttacgtt tagaaattga atcaatcaaa aagaaaatca aataatttgt
     1381 ggataacttt tagtttttta tcttttttat ccacattttt taaacaagct aaaaaacttg
     1441 atatgacttg tttaaaggct gttttccaca gatttcacag actctattat tactattatc
     1501 tttctaatac taaaaataaa taaaggagaa tccatgattc atttttcaat taataaaaat
     1561 ttatttctac aagcattaaa tactactaag agagctatta gttctaaaaa tgccattcct
     1621 attttatcaa cagtaaaaat tgacgtgacc aatgaaggta ttactttaat tggttcaaat
     1681 ggtcaaattt caattgaaaa ttttatttct caaaaaaatg aagatgctgg tttgttaatt
     1741 acttctttag gttcgatcct tcttgaagct tctttcttta tcaatgtagt atctagttta
     1801 cctgatgtaa ctcttgattt taaagaaatt gaacaaaatc aaattgtttt aaccagtggc
     1861 aaatcagaaa ttaccctaaa aggaaaagat agcgaacaat atccacgaat ccaagaaatt
     1921 tcagcaagca ctcctttaat acttgaaaca aaattactca agaaaattat taatgaaaca
     1981 gcctttgctg caagtacaca agagagtcgt ccgattttaa caggtgtcca cttcgtattg
     2041 agtcaacaca aagagttaaa aacagttgca acagactctc atcgcctaag ccagaaaaaa
     2101 ttgactcttg aaaaaaatag tgatgatttt gatgtcgtaa ttcctagccg ttctctacgc
     2161 gaattttcag cggtatttac agatgatatc gaaactgtag agattttctt tgccaataac
     2221 caaatcctct ttagaagcga aaatattagc ttctatactc gtctcctaga aggaaactat
     2281 cctgatacag atcgcttgat tccaacagac tttaacacta ctattacttt taatgtggta
     2341 aacttacgcc agtcaatgga gcgtgcccgt cttttatcaa gtgcgactca aaatggtact
     2401 gtgaaacttg aaattaagga tggggttgtt agcgcccatg ttcactctcc agaagttggt
     2461 aaagtaaacg aagaaatcga tactgatcag gttactggtg aagatttgac cattagtttc
     2521 aacccaactt acttgattga ttctcttaaa gctttaaata gcgaaaaggt gactattagc
     2581 tttatctcag ctgttcgtcc atttactctt gtgccagcag atactgacga agacttcatg
     2641 cagctcatta caccagttcg tacaaattaa gtgaaagagg ttgagcctgg ctcgcctctt
     2701 ttatgatata atcgaaaaag aaaaggagag tagtatgtat caagttggaa attttgttga
     2761 gatgaaaaaa tcacacgctt gtacaatcaa gtcgactggt aaaaaggcta atcgttggga
     2821 aattacacgt gtaggagcag atatcaaaat aaaatgtagt aattgtgagc atgttgtcat
     2881 gatggggcga tatgattttg agcgaaaaat gaataaaatt attgactgag aacccttagt
     2941 tagagggtta gcactttatc cctttttgtg ttataatatt agggattgaa atgaaaacgg
     3001 agaatgagaa atatggcttt gacagcaggt atcgttggtt tgccaaacgt tggtaaatca
     3061 acactattta atgcaattac aaaagcagga gcagaggcag caaactaccc atttgcgacg
     3121 attgatccaa atgttggaat ggtggaagtt ccagatgaac gcctacaaaa actaactgaa
     3181 atgataactc ctaaaaagac agttcccaca acatttgaat ttacagatat tgcagggatt
     3241 gtaaaaggag cttcaaaagg agaggggcta gggaataaat tcttggccaa tattcgtgaa
     3301 gtagatgcga ttgttcacgt agttcgtgct tttgatgatg aaaatgtaat gcgcgagcaa
     3361 ggacgtgaag acgcctttgt agatccactt gcagatattg ataccattaa tctggaattg
     3421 attcttgctg acttagaatc agtgaacaaa cgatatgcgc gtgtagaaaa gatggcacgt
     3481 acgcaaaaag ataaagaatc agtagcagaa ttcaatgttc ttcaaaagat taaaccagtc
     3541 ctagaagacg ggaaatcagc tcgtaccatt gaatttacag atgaggaaca aaaggttgtc
     3601 aaaggtcttt tccttttgac gactaaacca gttctttatg tagctaatgt ggacgaggat
     3661 gtggtttcag aacctgactc tatcgactat gtcaaacaaa ttcgtgaatt tgcagcgaca
     3721 gaaaatgctg aagtagtcgt tatttctgcg cgtgctgagg aagaaatttc tgaattgaat
     3781 gatgaagata aaaaagagtt tcttgaagcc attggtttga cagaatcagg tgtagataag
     3841 ttgacgcgtg cagcttacca cttgcttgga ttgggaactt acttcacagc tggtgaaaaa
     3901 gaagttcgcg cttggacttt caaacgtggt atgaaggctc ctaaagcagc tggtattatc
     3961 cattcagact ttgaaaaagg ctttattcgt gcagtaacca tgtcatatga agatctagtg
     4021 aaatacggat ctgaaaaggc cgtaaaagaa gctggacgct tgcgtgaaga aggaaaagaa
     4081 tatatcgttc aagatggcga tatcatggaa ttccgcttta atgtctaaaa attaataaat
     4141 ggtgttaatt aggttggaaa aaaattccaa cccttttggc ttttgaaagg aaaaataaat
     4201 gaccaaatta cttgtaggct tgggaaatcc aggggataaa tattttgaaa caaaacacaa
     4261 tgttggtttc atgttgattg atcaactagc gaagaaacag aatgtcactt ttacacacga
     4321 taagatattt caagctgacc tagcatcctt tttcctaaat ggagaaaaaa tttatctggt
     4381 taaaccaacg acctttatga atgaaagtgg aaaagcagtt catgctttat taacttacta
     4441 tggtttggat attgacgatt tacttatcat ttacgatgat cttgacatgg aagttgggaa
     4501 aattcgttta agagcaaaag gctcagcagg tggtcataat ggtatcaagt ctattattca
     4561 acatatagga actcaggtct ttaaccgtgt taagattgga attggaagac ctaaaaatgg
     4621 tatgtcagtt gttcatcatg ttttgagtaa gtttgacagg gatgattata tcggtatttt
     4681 acagtctgtt gacaaagttg acgattctgt aaactactat ttacaagaga aaaattttga
     4741 gaaaacaatg cagaggtata acggataaat ggtgacctta ttagatttat tctcagaaaa
     4801 tgatcagatt aaaaaatggc atcaaaattt aacagataag aaaagacaac taatacttgg
     4861 tttatcaaca tctactaagg ctcttgcaat tgcaagcagt ttagaaaaag aagataggat
     4921 tgtgttattg acgtcaactt atggagaagc agaaggactt gttagtgatc ttatttctat
     4981 cttgggtgag gaactcgtct atccattttt ggtagatgat gctcctatgg tggagttttt
     5041 gatgtcttca caggaaaaaa ttatttcacg ggttgaagcc ttgcgttttt tgactgattc
     5101 atctaagaaa gggattttag tttgtaatat cgtagcaagt cgattgattt taccgtctcc
     5161 caatgcattc aaagatagta ttgtaaaaat ctcagttggt gaagaatatg atcaacacgc
     5221 gtttatccat cagttaaagg aaaatggcta tcgaaaagtt actcaagtac aaactcaggg
     5281 cgaatttagt cttcgaggag atattttaga tatttttgaa atatcccagt tagaaccttg
     5341 tcgaattgag ttttttggtg atgaaattga tggtatcagg tcatttgaag tagaaacaca
     5401 attatcgaaa gaaaataaga cagaactcac tatctttcca gctagtgata tgcttttgag
     5461 agaaaaggat tatcaacgag gacagtcagc tttagaaaaa caaatttcaa aaactttatc
     5521 acctattttg aaatcatacc tagaagaaat tctttcaagt tttcaccaaa aacaaagtca
     5581 tgcagactct cggaagtttt tatctttgtg ctatgataag acatggactg tctttgatta
     5641 tattgaaaaa gatactccaa tattctttga tgattatcaa aaattgatga atcagtatga
     5701 agtctttgaa agagacttag cgcagtactt tacagaagaa ttacagaata gtaaagcatt
     5761 ttctgatatg cagtattttt ctgatattga acaaatctat aaaaaacaaa gtccagtgac
     5821 ctttttctct aatcttcaaa agggtttagg aaatc
//
LOCUS       DAVMHG010000124         5513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMHG010000124 DAVMHG010000000
VERSION     DAVMHG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.126"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..930)
                     /locus_tag="VYR08_001767"
     CDS             complement(<1..930)
                     /locus_tag="VYR08_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV3467255.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSSSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(927..1784)
                     /locus_tag="VYR08_001768"
     CDS             complement(927..1784)
                     /locus_tag="VYR08_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV3467256.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(1788..3134)
                     /locus_tag="VYR08_001769"
     CDS             complement(1788..3134)
                     /locus_tag="VYR08_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3467257.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(3147..3431)
                     /locus_tag="VYR08_001770"
     CDS             complement(3147..3431)
                     /locus_tag="VYR08_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3467258.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(3435..5465)
                     /locus_tag="VYR08_001771"
     CDS             complement(3435..5465)
                     /locus_tag="VYR08_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV3467259.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIEKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         1855 a         1161 c          744 g         1753 t
ORIGIN      
        1 tcttgcatta taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc
       61 attaagaaga taatccattt tccctacttg accaaatctt tcttgaacac ccatccgatg
      121 aatttttgtt attccatcat cagagaataa ttcacataaa gcactgccaa ttccacctat
      181 ctgattgtgg ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc
      241 tggtatcggt ttgattctaa ataaatctat cacacctact gaataaccta atttagacag
      301 ttcatctgca actcgaatac ttggagcaac cattatgcca gaagcaacga ttacaagatc
      361 ttcaccatgt cttaactcaa tgtagccttt agaaaaatct tctccacctt gatatacagc
      421 ctctggagct tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctgatt
      481 caatatttca cgaaattgga tatcatcagt tgcttcgaaa atgattgatt taggaattaa
      541 acgtaacaag ccaatttctt caaatggcat atgtgtccca ccattcattt ctgcagttac
      601 tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa
      661 ttgatcaaat acccttcgtg aagaaaaagg accaaatgta tgaagataag gtctaaaccc
      721 ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc caacattcac
      781 ataacggtct ccaaagtcct tttcaagatt atgagtagcc atcgaacttg acaaatcggc
      841 ttctaagact actatatcag aagaactttg attagcctct aaaaggaagt ctctatatac
      901 atgccgtaat tctttcgtac ttctcatcat tctgtttcct ccaattcctg acttaatctt
      961 tctacaactg aagttaacat ttgtttctcc tctacagtag ggcgaagatg atgattggat
     1021 ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta
     1081 ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct
     1141 gaacccttga ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct
     1201 ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca
     1261 attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca
     1321 tttaactcac catctccaac aatagcataa gtataaaagg gactctttct tattctctga
     1381 ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct
     1441 atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa
     1501 gaatatagga attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt
     1561 cctccatgac cttttgataa tatgaaataa tctctatctc gtgctgcaaa tatttctgga
     1621 gtcattggca ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct
     1681 ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagtc
     1741 gcaaattttc ttaactcatc ttctctattt ttacttaaaa tcatccctta ttcctccttt
     1801 gcagatggct ttttaataaa ggatactcca aacataactg ctagaataag aacaagacca
     1861 atcacaatgc ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga
     1921 ccaaaatctg catctgagaa agttgatcct tggaaaccaa gtcctcccaa aactggcatt
     1981 aaaaagactg gaagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca
     2041 cgaacaccac cagatgcatt cccaatgaca cctgcagtcg ctccacagaa gaaatgaggc
     2101 acaacacctg gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa
     2161 ccaccaataa aactagagat aaatccaatt agaactgcat tgggtgcata agtataaaca
     2221 atcggacaat ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag
     2281 gctggaacaa tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct
     2341 gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct
     2401 ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatagat
     2461 aaagtgatac taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc
     2521 tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc
     2581 aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt
     2641 tgcacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagatg
     2701 agaggcaagc tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat
     2761 agagtgtggt gccctgttaa aaaaatatat ttaaatcgag taaaacgagc gattaagata
     2821 ttgaacacca tgcctgcaaa cataatcatt gcagtagctg agccatatgt tgttaaagct
     2881 acagctacaa ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac
     2941 atggtaccaa atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag
     3001 aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga
     3061 actaatccta aaattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat
     3121 atgaacttca tcatgacgct agcctcctat ataagtcctt tttcttcaca aagtttagta
     3181 attaattctc gtagttcatc catatcaata atactattta agatacgaac atctccaaga
     3241 tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct
     3301 gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca
     3361 gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca
     3421 atttttaaca ttatctaatc ctcctgttta attatcattt taatgtcatc atagtttttt
     3481 gatgaaatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa
     3541 gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt
     3601 tctggattat ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca
     3661 ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa
     3721 ggtccaaact cttctacttt ttcaatcatt gcctcagggt agttctccgt tatcttatct
     3781 tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa
     3841 ctaacctgat aggtttcttt ggtaataagt tgttctagca ctggtacaat ttcctttcta
     3901 tcattttttt gataaagata attctttaac gccaatctta attccaattc ttgtgtaata
     3961 attccatatc ttttgacaat attcaggatt tgttcaatct caaaatctcc atactctaaa
     4021 ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata
     4081 accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca
     4141 tagtcactac tagctttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt
     4201 tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac accattagga
     4261 caaatgattg ctgctttata gcatttttga ggcaaagtat ctgctttctt taaataacct
     4321 ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc
     4381 atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca
     4441 tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta agccggtata
     4501 aggtggcggc gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata
     4561 tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct
     4621 acttcacctt caaagcaact tgataataaa actgtgatat agcgataatc atcctcagaa
     4681 aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct
     4741 tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac
     4801 ctacacagaa tgaatactaa accgaaaagg taaactttta attgattaac aataggtact
     4861 agctgtagct tctcataata atctttaact acctgatcaa tcaaatcata agttaatgaa
     4921 tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac
     4981 tcactaacca tttgaaaagc taaacggtgc ttattccact ctgaaccgtg taaagtataa
     5041 ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat
     5101 tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc
     5161 gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt
     5221 ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat
     5281 aaactatagc gaccttgttt tttatccagc actatccctt tattagctag ataaggcact
     5341 aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca
     5401 tataatgata gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca
     5461 aacatttctc ttcctttcta actacaatta tagtgtaaaa attataagaa taa
//
LOCUS       DAVMHG010000125         5403 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMHG010000125 DAVMHG010000000
VERSION     DAVMHG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.127"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            95..343
                     /locus_tag="VYR08_001772"
     CDS             95..343
                     /locus_tag="VYR08_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467260.1"
                     /translation="MVSSSSSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            474..1646
                     /locus_tag="VYR08_001773"
     CDS             474..1646
                     /locus_tag="VYR08_001773"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV3467261.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1646..2920
                     /locus_tag="VYR08_001774"
     CDS             1646..2920
                     /locus_tag="VYR08_001774"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV3467262.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMAILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2956..3921)
                     /locus_tag="VYR08_001775"
     CDS             complement(2956..3921)
                     /locus_tag="VYR08_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV3467263.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTQEKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3902..5356)
                     /locus_tag="VYR08_001776"
     CDS             complement(3902..5356)
                     /locus_tag="VYR08_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV3467264.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWVQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAINNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRVLAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNSQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
BASE COUNT         1514 a         1102 c         1236 g         1551 t
ORIGIN      
        1 attatcaatc atgaactagg attctagttt tgcaggaagt ctactacttt ccttattgtc
       61 tgtaaatcta ctgaccttgt tgtttatccc agtcatggtt tctagttcga gctcagagtt
      121 tcaaagtgga tggcaagagc atcaattgat tgctgagaag gttagtaaaa cacttgataa
      181 gacatttgat aaggatgtca gagaaattcc gaccagtcag ttttatcaaa aatttgtaga
      241 tgagatggga aggacttact caggaaattt gatcctccag gagctgataa ctgtgaatgg
      301 agcttataaa gctacttata tcggtgaact ctctagcaac taatcattct ttaaaatgat
      361 gaccaataag aggcttgatt tggtgaatca agtctttttt ctctctttta ctgccatttg
      421 tgataaaata gtatagaacg attttttaca tgaatgataa aacagaggta aatatgacaa
      481 tcggtattga taagattggt tttgcgacca gtcaatatgt cttgaaatta caagacttag
      541 cagaagcgag gggaattgac cctgaaaaat taagtaaagg actcttactc aaggaattga
      601 gtattgcgcc cctaactgag gacattgtga ccttggcggc cagtgctagt gactctattt
      661 taactgagca agaaagacaa gaagttgaca tggtcattgt ggctaccgag tcaggaattg
      721 accagagtaa ggctgcggcc gtctttgtgc atggcttgct gggcatccag ccctttgctc
      781 gtagtttcga gattaaagaa gcctgttatg gagcgactgc tgcccttcat tatgccaaat
      841 tgcatgtgga aaattctccg gagtccaagg tcttggtcat tgccagtgat attgccaaat
      901 acggtattga aactccagga gaaccaactc aaggtgctgg aagtgtagct atgttgatta
      961 cacaaaatcc acgcatgatg gcctttaata atgacaatgt agctcagacc cgtgacatca
     1021 tggatttctg gcgaccaaat tactcgacaa ctccttatgt aaatggtgtt tattctaccc
     1081 aacaatactt ggatagtttg aaaacgactt ggcttgaata tcaaaaacgc taccagctta
     1141 ctctggatga ttttgcggct gtttgtttcc acttgcctta tcctaaatta gcgctaaaag
     1201 gcttgaaaaa aatcatggat aagagcctgc ctcaagagaa aaaagacctc ttacaaaagc
     1261 attttgacca gtctattctc tacagtcaaa aggtggggaa tatctacaca ggttcacttt
     1321 tccttggact tttgtctctc ttggaaaata cagatagctt gaaagctggg gataaaatcg
     1381 ccctttatag ttacggaagt ggagctgtgg ctgagttctt cagtggtgaa ttggttgaag
     1441 gatatgaagc ttatttggat aaagaccgct tgaacaagct caaccaacga actgccctat
     1501 ccgttgcaga ctatgaaaag gtcttttttg aggaagtaaa cttggatgaa acaaactctg
     1561 cccagtttgc tggctatgaa aatcaagatt ttgccttggt tgaaattctt gaccaccaac
     1621 gccgttatag caaggttgaa aaataatgaa gataagttgg aatggatttt ctaaaaaatc
     1681 ataccaagag cgcctcgagc tgctaaaagc tcaggcgctc cttagtcctg agagacaagc
     1741 tagtctggag aaggatgaac agatgagtgt gactgtggca gaccagctga gtgagaatgt
     1801 ggtgggaact ttttctctgc cttattcgct ggttccggag gtacttgtca acggtcagga
     1861 atacaccgtt ccctatgtga cagaagaacc ctctgtggtt gcggcggcca gctatgccag
     1921 caaaatcatc aagcgtgcag gtggttttac tgcacaagtc catcagcgcc agatgattgg
     1981 gcaggtagcc ctttatcaaa ttgctaatcc taaactagcg caagagaaga ttgccagcaa
     2041 gaaagcggag ctcttggagc ttgccaatca agcctatcct tctatcgtta aacgtggagg
     2101 tggggcgcgt gatctgcatg tcgagcagat aaaaggtgaa ccagactttc tcgttgttta
     2161 tattcatgtc gatacccagg aagccatggg tgccaatatg ctcaacacca tgctggaagc
     2221 cttgaaacca gtcttagaag aactcagtca gggacagagt ctcatggcaa tcctgtccaa
     2281 ctacgcgacc gattctctgg tgactgcaag ctgtcgcatc gcctttcgct acttgagccg
     2341 ccaaaaggat caaggacgag agattgcgga gaaaattgcg ttggctagtc agtttgcgca
     2401 ggctgatcct taccgagctg ctactcataa taaaggaatt tttaatggta ttgatgcgat
     2461 tttgattgcc actggtaatg actggcgtgc catcgaagct ggggcccatg cctttgccag
     2521 tcgagatgga cgctatcaag gtcttagctg ctggacgctg gaccttgaaa gagaagaatt
     2581 ggtcggtgag atgaccctgc ccatgcctgt agcgactaag ggtggctcta tcggcctcaa
     2641 cccacgtgta gctctcagtc atgatctact aggaaatcct tctgccagag aattagccca
     2701 gattatcgtg tccatcggtc ttgcccaaaa ttttgcagcc ctcaaagcct tggtaagtac
     2761 gggcatccag caaggccata tgaaactaca ggccaaatcc ctagctctcc tagctggggc
     2821 tagtgaatct gaagttgctc ccctagtaga gcgcctcatc tcagataaaa cctttaacct
     2881 agagacagcc cagcgctatc tcgaaaattt aagatcataa aaaactcaga cgaatcggtc
     2941 tgagttttct tgtgtttaaa tactttttcc tggtaatagt gtaactggta agaagtaacc
     3001 agttgtggcg acttccttgc cttcaatctt ttgcaaaaga agatcaatag tgagacaagc
     3061 aatctcttcc aaaggttgct tgatagtagc caattgaggg tagtaatttt cgataaagta
     3121 ggtcccatca tagccgatga ccttgagctc ttttgggaca gaaatgccca attcttgagc
     3181 gattttaatg accagaatag ctgtcaaatc atccgaagca aaaatggcat ctggtttttc
     3241 ctgggtcaag atattcttga tttccatctc ttttctgacg ggagaaaagt cactggaaac
     3301 attgataata ggagcttttg ggagtacgga tgcaaaacca gcgtggcgca gtccggttgg
     3361 cgaattggaa ttgtcattcc ctgtaatcat gatgatagac tgggcacctg tcttgaccaa
     3421 ggtttgggca gcaagaaccc caccagcata gttgtcagag gagacgacag ggatgtctgg
     3481 cgataggttt cggtcaaagg aaataatcgg cgctgtcaca cgattgtagt cttcgattcc
     3541 taggttgtga ctaccagaaa tgatgccgtc cacctgattg gcttccaaca tttcgatgta
     3601 ttcgcgttcc ttctcagaat catgttcact gttgcagatg atggtcttgt aaccattttt
     3661 gaagagttgg tgttccaatt tatcaatcaa ttctgcatag aaaacattgg aaatattggg
     3721 gaaaatcaag ccgattaact tagctgattt tccttgcaga ctacgagcca ggttgttggg
     3781 tttatagccc aattctcgca tggcttcatt gactttttgg atggttttct cagatagata
     3841 ccctttttta ttgataaccc gagaaacggt agtaggactg acgcctgcaa gtttggcgac
     3901 atcagttagt tttgcgacca taatctaatt catagtaagt tccagttggg tttccagatt
     3961 tgatcaggat accattttgg tccgcatgtg ggaagacacg accagaaaat actttttctc
     4021 ctttattgat gaaaatttca aagacagagt tatcgatgaa gattgtagca gtagtagcct
     4081 gattctcgat agggcaagaa cgagttgtcc caaattcttg ggcatactgt tctccagcct
     4141 ggctacgatc cactgtcact tgactgttta caaggtcaaa gttgattgaa agtcccttac
     4201 cttctttatc agcaagtaag acaatctcgc tctggctatt agcttccaag ttgagttcaa
     4261 gttcgtaagt gttcttggtt tgggaacggt ttgagaaggc ttcttcagaa gcacgaaggt
     4321 ccttaatagc agcgactgga tactggtaga gcttgtcgtc tttgatagtg agttccttga
     4381 ccaaagagaa ggttccttgg tggtcaaaac ggtcagatgg gtaagaaaca tctggcaaac
     4441 caagccagct cactgctaga acacgcccat caggagcgtt gaaggcttga gttgcatagg
     4501 cttcgaaacc gtaatccatg ttttgaagtt gagacacatc taccattttg gcatttttag
     4561 ggtcaaagga agccccaatc ttatacatat ttggaaagat attatcgtag tctagaactt
     4621 tcttatccaa tccttgtgga cagtagagaa ggacaggctg ttcctctaca aagaccagat
     4681 taggacattc catcatgtag gcagtacggt cgttagcaaa gtcaaggtcg ccaactgctt
     4741 gccagtttgt gtagtcgtta ttgatagcct tgtagagacg gacgaagcct tttttctcca
     4801 agtcctgtcc accgacgaca gcatagtatt gacctttaaa gttaaaaatt tgtggatcgc
     4861 ggaagtggtc agtagagtct gctggctggt caatcaagat cttgtcaatc tttgtaatct
     4921 taccctcctt gtccatcaaa gcaccgatct ggtatgggtg acggatccag ttttcatcgc
     4981 gaacatttcc tgtataaaat aggaataagt tatcgccaaa ttgcatggca gaaccagagt
     5041 aggcaccgtg gctatctaat ggagtatctg gtaaaacttt gattccagtt tctttaaagt
     5101 gaaccaaatc atcactttct agctgtaccc aagatttcaa accgtgggct gcaccaaaag
     5161 gaaaattctg gtaaaagagg atccacttgc catcaaagta agaaaagcca tttgggtcgt
     5221 tgagaagtcc tgtttttggc tcaacatggt aatgagtatg ccatggagat tgtgccatat
     5281 tttcctttat atgttgaatt tcttcttgcg tccaatcttg ataaagtctg taacgacgct
     5341 cagttgtcca ttccattcat tcctccaaaa atcttatcac ttttaatagt aactgttttc
     5401 gat
//
LOCUS       DAVMHG010000126         5370 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMHG010000126 DAVMHG010000000
VERSION     DAVMHG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.128"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            161..1168
                     /gene="gap"
                     /locus_tag="VYR08_001777"
     CDS             161..1168
                     /gene="gap"
                     /locus_tag="VYR08_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV3467265.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(1236..2072)
                     /locus_tag="VYR08_001778"
     CDS             complement(1236..2072)
                     /locus_tag="VYR08_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV3467266.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            2198..4393
                     /locus_tag="VYR08_001779"
     CDS             2198..4393
                     /locus_tag="VYR08_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV3467267.1"
                     /translation="MKLDELFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            4446..4598
                     /gene="rpmG"
                     /locus_tag="VYR08_001780"
     CDS             4446..4598
                     /gene="rpmG"
                     /locus_tag="VYR08_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3467268.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            4608..4784
                     /gene="secE"
                     /locus_tag="VYR08_001781"
     CDS             4608..4784
                     /gene="secE"
                     /locus_tag="VYR08_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV3467269.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            4839..>5370
                     /gene="nusG"
                     /locus_tag="VYR08_001782"
     CDS             4839..>5370
                     /gene="nusG"
                     /locus_tag="VYR08_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV3467270.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         1672 a          953 c         1167 g         1578 t
ORIGIN      
        1 ttacagtctt aaaaagaaac taacatagaa aaacaatggc catacttgca attcaactca
       61 aatagttgta taataggttt gttgaaaggt gatttgtagt gattcaagtt actcttttca
      121 caataaaaat ttcatgattt tcataaggag gaaatcacta atggtagtta aagttggtat
      181 taacggtttc ggacgtatcg gtcgtcttgc tttccgtcgt atccaaaacg tagaaggtgt
      241 tgaagttaca cgcatcaacg accttacaga tccagttatg cttgcacact tgttgaaata
      301 cgacacaact caaggtcgtt tcgacggtac tgttgaagtt aaagaaggtg gatttgaagt
      361 taacggtaaa ttcatcaaag tttctgctga acgtgatcca gaacaaatcg actgggctac
      421 tgacggtgta gaaatcgttc ttgaagctac tggtttcttt gctaagaaag aagcagctga
      481 aaaacacctt aaaggtggag ctaaaaaagt tgttatcact gctcctggtg gaaacgacgt
      541 taaaacagtt gtattcaaca ctaaccacga cgttcttgac ggtactgaaa cagttatctc
      601 aggtgcttca tgtactacaa actgcttggc tccaatggct aaagctcttc aagacaactt
      661 tggtgttgtt gaaggattga tgactactat ccacgcttac actggtgacc aaatgatcct
      721 tgacggacca caccgtggtg gtgaccttcg ccgtgctcgc gctggtgctg caaacatcgt
      781 tcctaactca actggtgctg caaaagctat cggtcttgta atcccagaat tgaatggtaa
      841 acttgatgga tctgcacaac gcgttccaac tccaactgga tcagttactg aattggtagc
      901 agttcttgaa aagaacgtta ctgttgatga agtgaacgca gctatgaaag cagcttcaaa
      961 cgaatcatac ggttacacag aagatccaat cgtatcttca gatatcgtag gtatgtctta
     1021 cggttcattg tttgacgcaa ctcaaactaa agttcttgac gttgacggta aacaattggt
     1081 taaagttgta tcatggtacg acaacgaaat gtcatacact gcacaacttg ttcgtactct
     1141 tgaatacttc gcaaagattg ctaaataatt cttgagttga tagaaagcaa ggctttgtgg
     1201 tcttgctttt ttatatggaa aaatggatga cacgatcatc cattcttttt taattctttt
     1261 tcaaatgtat ttgaaagggt agtgaaagtt agcctctcta aagtaagtgg gtgggtaaag
     1321 gaaagtcgga aggcatgaag cataagccgg cttgtctttg atttactatt atagagaggg
     1381 tctcccagga taggaagatt atgatgcaaa aggtgcacac gaatctgatg ggttcgccct
     1441 gtctttagct tgcaatgagc caaggaagtc ttgtttgaga attgctttaa tctgcttaca
     1501 tgcgtttcag catattgccc attttttgca tcaactattc tttttctacg atcatggcga
     1561 tcacgtccaa ttttgtctct gaaaacaagt tcttttctgt tgatatttcc atcaactaga
     1621 gcccaatatt ctctagaaat ctcttttttc tccaataagc gattgagaat gggcaggata
     1681 aaaggatttt tggcaaagag aactaagcca ctggtttcca tgtccagacg atgaacgaca
     1741 tagcaggttt ggccaacata ggtactgaca tggttaagaa gggcaatttc gtttggttga
     1801 ttaccatgcg ttttcatccc ctctggtttg tttacaataa tcaagtgttg atcttgataa
     1861 acttcctgca ctaagtctgg gttgccccaa gggatcgtct tttgggaata atcttcctcg
     1921 tcaaaagtca actggcaaac atctccagga tttacgattt cgttccagtg gacttcttct
     1981 tgatttatca aaatatgttt cttgattctc aaaaaatgac ggatttttct agggatgagg
     2041 agttgttcct caagtaattg ctttaccgtc atttgaggta gagaggcggg taatgtaaat
     2101 gtgaattgca tacagatatt gtaacaaaaa aagccctatt tggataggaa atagctaaat
     2161 tcttgtcttc ctatgatgaa gatgataaaa taaacgcatg aaattagatg aattatttga
     2221 gaaatttctt tctcttttta aaaaagaaac aagtgaacta gaggactctg attctactat
     2281 cttacgtcgc tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga ttcgaaaatt
     2341 ctggcgtcgt tatcatctaa caaagattat ccttatacta ggtttgagtg caggattgct
     2401 agttggaatc tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt tgcaaaatgc
     2461 cttgaaaact cggactctta tttttgaccg tgaagaaaaa gaggctggtg ccttgtctgg
     2521 tcaaaaggga acctatgttg agctgactga catcagtaaa aacttgcaga atgctgttat
     2581 tgcgacagaa gaccgttctt tctataaaaa tgacgggatt aactatggcc gtttcttctt
     2641 ggctattgtc actgctggac gttcaggtgg tggctctacc attacccaac agctggctaa
     2701 aaacgcctat ttatcgcagg atcaaactgt tgagagaaaa gcgaaagaat ttttccttgc
     2761 cttagaatta agcaaaaaat atagtaagga gcaaattcta accatgtacc ttaacaacgc
     2821 ttattttgga aatggtgtgt ggggtgtaga agatgcgagt aagaaatact ttggagtttc
     2881 tgcatcagaa gtgagtctgg atcaagctgc gactctggca gggatgctca aggggccgga
     2941 actgtataat cccttgaatt ccgtagaaga ttctactaat cggcgcgata ctgtcttgaa
     3001 gaatatggtt gcagcaggat atattgataa aaaccaagaa accgaagctg ctgaagttga
     3061 tatgacttcg caattgcacg ataagtatga aggaaaaatc tcagattacc gttacccctc
     3121 ttattttgat gcggtggtta atgaagctgt ttccaagtat aatctaacag aggaagagat
     3181 tgtcaataat ggctaccgca tttacacaga gctggaccaa aactaccaag caaatatgca
     3241 gattgtttat gaaaacacat cgctatttcc gagggcagag gatggaacgt ttgctcaatc
     3301 aggaagtgta gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg gtcaagttgc
     3361 tgacaatgat aaaactggat tccggaattt caactatgca acccaatcaa agcgtagtcc
     3421 tggttctaca attaagcctt tagttgttta tacaccagca gttgaagcag gctgggcttt
     3481 gaataagcag ttggataacc ataccatgca gtatgatagc tataaggttg ataactatgc
     3541 agggatcaaa acaagtcgag aagttcctat gtatcaatcc ttggcagaat cgcttaatct
     3601 acctgctgtt gccactgtta atgatttggg tgttgacaag gcttttgagg caggcgaaaa
     3661 attcggactc aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg gaagcggtgt
     3721 tgaaaccaac cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg aaggtttaat
     3781 gcctgaagct cattttatta gtagaattga aaatgctagt ggacaagtta ttgcgagtca
     3841 taaaaattca caaaaacggg tgattgataa gtctgtagct gacaagatga ccagtatgat
     3901 gttggggact ttcaccaacg gtaccggtat tagttcatcg cctgcagact atgtcatggc
     3961 agggaaaact ggaacaactg aagcagtttt caatccggag tacacaagtg accagtgggt
     4021 aattggttat actccggatg tagtgattag ccactggctt ggctttccga ccactgatga
     4081 aaatcactat ctagctggct ctacttcaaa cggtgcagct catgtcttta gaaacattgc
     4141 caataccatt ttaccttata cgccaggaag tacctttacg gttgaaaatg cttataagca
     4201 aaatggaatt gcaccagcca atacaaaaag acaagtacaa accaatgata atagccagac
     4261 agatgataat ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg aggctagccg
     4321 ggctatctca gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt cgatagtcaa
     4381 tctatttcgc taagatgctt gtcaaagcct agctttcttg ttataatgga taagatggag
     4441 gcgttatggc actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg agaaactatt
     4501 caatcaagat cagcggaaac cccaagccta cacgactaga agtaaataaa ttttgtaagc
     4561 attgtggcaa gtacactaca cacagagaaa cgagatagga gagagcgatg cgttttattg
     4621 gagatatttt tagacttctt aaagacacaa catggccaac tcgcaaggaa agctggagag
     4681 attttcgttc tatcatggaa tacacagctt tctttgtagt aattatttac atttttgacc
     4741 agttgattgt ttcaggtttg attcgattta ttaacatttt ttagaagatt agtggagtta
     4801 attacactag aaatcttcta tttatgaaag gaaatatcat ggatagtttt gataaaggat
     4861 ggtttgtttt acaaacttat tctggttatg aaaataaggt gaaagaaaat ctattacaac
     4921 gtgcacaaac ctacaatatg ttggataata ttctacgcgt tgaaattcca acacaaacag
     4981 tgcaagttga aaaaaatgga aagagaaaag aagtagaaga aaatcgcttt ccaggttatg
     5041 ttcttgtaga aatggtcatg acagatgaag cttggtttgt tgttcgaaac acaccaaatg
     5101 ttacaggatt tgtcggatca cacgggaaca gatcaaaacc aactccatta ttggaacaag
     5161 aaattcgtga cattttggta tctatgggac aaactgttca agaatttgat ttcgatgttg
     5221 agattggtca aactgtacgt attattgatg gtgcttttgc agactacact ggtaagatta
     5281 cagaaattga taataacaaa gtgaaaatga ttatctctat gtttggtaat gacacagttg
     5341 cagaagtaaa cctaaaccaa atcgcagaat
//
LOCUS       DAVMHG010000127         5287 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMHG010000127 DAVMHG010000000
VERSION     DAVMHG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.129"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..825
                     /locus_tag="VYR08_001783"
     CDS             1..825
                     /locus_tag="VYR08_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV3467271.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            847..1586
                     /locus_tag="VYR08_001784"
                     /pseudo
     CDS             847..1586
                     /locus_tag="VYR08_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1599..2765
                     /locus_tag="VYR08_001785"
     CDS             1599..2765
                     /locus_tag="VYR08_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV3467272.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            3273..4766
                     /locus_tag="VYR08_001786"
     CDS             3273..4766
                     /locus_tag="VYR08_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV3467273.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4779..>5287
                     /locus_tag="VYR08_001787"
     CDS             4779..>5287
                     /locus_tag="VYR08_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467274.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLV"
BASE COUNT         1686 a          938 c         1118 g         1545 t
ORIGIN      
        1 atgaatcaat cctactttta tctaaaaatg aaagaacaca aactcaaggt tccttataca
       61 ggtaaggagc gccgtgtacg tattcttctt cctaaagatt atgagaaaga tacagaccgt
      121 tcctatcctg ttgtatactt tcatgacggg caaaatgttt ttaatagcaa agagtctttc
      181 attggacatt catggaagat tatcccagct atcaaacgaa atccggatat cagtcgcatg
      241 attgtcgttg ctattgacaa tgatggtatg gggcggatga atgagtatgc ggcttggaag
      301 ttccaagaat ctcctatccc agggcagcag tttggtggta agggtgtgga gtatgctgag
      361 tttgtcatgg aggtggtcaa gccttttatc gatgagacct atcgtacaaa agcagactgc
      421 cagcatacgg ctatgattgg ttcctcacta ggaggcaata ttacccagtt tatcggtttg
      481 gaataccaag accaaattgg ttgcttgggc gttttttcat ctgcaaactg gctccaccaa
      541 gaagccttta accgctattt cgagtgccag aaactatcgc ctgaccagcg catcttcatc
      601 tatgtaggaa cagaagaagc agatgataca gacaagacct tgatggatgg caatatcaaa
      661 caagcctata tcgactcgtc gctttgctat taccatgatt tgatagcagg gggagtacat
      721 ctggataatc ttgtgctaaa agttcagtct ggtgccatcc atagtgaaat cccttggtca
      781 gaaaatctac cagattgtct gagatttttt gcagaaaaat ggtaagttaa gaaaggaaaa
      841 aacgaaatgc atattgaaca tcttagccac tggagtggtc atcttaaccg tgaaatgtac
      901 cttaaccgtt atggacatgg tgggattcca gttgtggtct ttgcttcatc aggtggtagt
      961 cacaacgaat actatgattt tggcatgatt gatgcctgtg cttcctttat cgaggaaggc
     1021 cttgtccagt tctttaccct atctagtttg gatagtgaga gctggttggc tacttggaaa
     1081 aatgctcatg accaagcgga aatgcaccgt gcctacgaac gttatgtgat tgaggaggcc
     1141 attcttttat caagcacaag acaggttggt ttgatggcat gatgacgaca ggttgctcta
     1201 tgggagccta tcatgcactc aatttcttcc tccagcatcc agatgtcttt accaaagtga
     1261 ttgctctcag tggtgtttac gacgcacgtt tctttgtcgg tgattactac aacgatgatg
     1321 ctatttacca aaactcgcca gtagattata tttggaacca aaacgacggc tggtttattg
     1381 accgttaccg tcaggcagag attgtgctgt gtacggggct tggagcctgg gaacaagatg
     1441 gtttgccatc cttttacaag ctcaaagaag cctttgacaa gaaacaaatt ccagcctggt
     1501 ttgctgaatg gggacatgat gtcgcccatg actgggaatg gtggcgtaaa caaatgcctt
     1561 atttcctcgg taatctctat ttataaaagg agttacctat gaattacctt gttatttctc
     1621 cctactatcc acaaaacttt caacagttta ccatcgaact agctaataaa ggcatcacag
     1681 tcttgggaat tggtcaagag tcttacgagc aattggatga gcccttgcgc aatagcttga
     1741 ccgagtattt tcgtgttgat aatcttgaga acatagatga agtcaaacgt gcagttgctt
     1801 ttctctttta taaacatggt ccaattggcc gcatcgagtc tcacaatgaa tactggcttg
     1861 agctagacgc aacactcaga gaacaattca atgtttttgg tgccaaacca gaggatctca
     1921 aaaagacgaa atataagtct gaaatgaaga aacttttcaa aaaagcaggt gttcctgtgg
     1981 tacctggagc tgttatcaag acggaagcag atgttgatca agcagtgaaa gaaatcggtc
     2041 ttccaatgat tgccaaacct gataatggag tgggagcagc cgcaaccttt aaacttgaga
     2101 cagaagacga tatcaatcac ttcaagcaag aatgggacca ttcaaccctt tatttctttg
     2161 aaaaatttgt cacttccagc gaaatctgta cctttgacgg gctcgtggac aaggatggaa
     2221 agattgtctt ctcaacaacc tttgactacg cctatacacc gcttgacctc atgatttata
     2281 agatggacaa ttcttattat gtgctcaagg atatggatcc taaactgcgc aagtatgggg
     2341 aagcaattgt caaagaattt ggtatgaaag aacggttttt ccatattgag ttcttccgtg
     2401 agggggacga ttatattacc atcgagtaca ataaccgccc tgcaggtggt tttaccattg
     2461 atgtttataa ctttgctcat tccttggacc tttatcgtgg ctatgcagct attgtcgcag
     2521 gagaagagtt cccagcgtca gactttgaaa ctcagtattg tttggctact tctcgccgtg
     2581 caaatgctca ctatgtttat tcagaagagg atttgcttgc caaatatagc cagcagttca
     2641 aggttaaaaa agtcatgcca gctgccttcg cggaacttca aggagattac ctgtatatgc
     2701 tgaccactcc gagtcgacaa gaaatggagc agatgattgc agatttcgga caacgtcaag
     2761 aataagaact atcggattaa ggaaattaac tcccttaatc cttttgtttt gtctgataaa
     2821 aaataagagc atcccaacaa ggtagctatc ataaaacttg ttcgataact atttgaagca
     2881 ggattaggtg gtcagaaatt aaattttaat atttcaattg agtcatagta ttgtgtttgc
     2941 gtatccttaa atcagctaaa aggatccatg acgacaccta tacgatatag ttttcaagat
     3001 accaaacaag tctattaata ttcaatgaaa atcaaagagc aaactaggaa gctagccgca
     3061 ggtttctcaa aacactgttt tgaggttgtg gatagaactg acagagtcag tatcatatac
     3121 ctacggcaag gtgaagctga cgtggtttga agagattttc gaagagtata aaatattcag
     3181 gtgacgcata gatatagtta attgaagctt tgtttgaaat ctgataaaat aatgatatta
     3241 ctaagtttta aaaactaaag aaaagggaag atatgattac aggcgaatta aaaaataaaa
     3301 tcgatcagct gtgggaaatt ctttggacag aaggaaacgc aaatccttta acaaatattg
     3361 aacagttgac ttatctctta tttatgaaag atttggatag tgtcgagctt ggacgtgaaa
     3421 gtgatgctga atttctaggg attccttatg agggagtttt tccaaaagat aaacctgaat
     3481 accgttggtc aacttttaaa aatataggag atgctcagga agtttatcgt ttaatgactc
     3541 aggagatttt tccgtttatt aaaaatctca agggggatac agatgataca gccttttcac
     3601 gatatatgcg agaagctatt tttcaaataa ataaacctgc tacgcttcaa aaggcaattt
     3661 ctatcttaga tgtttttcca actaggggat tagatgtaga ttttgataat gacaaacaaa
     3721 gtattactga tatcggagat atctatgaat atctgttatc aaaattgtcg accgcaggta
     3781 aaaatggaca gttccgtaca cctcgtcaca tcatcgatat gatggttgag ttgatgcaac
     3841 cgactatcaa agatatcatc tcagatcccg ctatgggttc tgctggcttc ttagtatctg
     3901 ctagccgtta cttaaagcgt aagaaagatg aatgggaaac caatacagat aatatcaatc
     3961 attttcataa tcagatgttt catggaaatg atacggatac gactatgttg agacttgggg
     4021 cgatgaacat gatgctacat ggagtagaaa atccacaaat cagttacctt gactcgctgt
     4081 ctcaagataa tgaagaagcc gataaatata ctttggtttt agcaaatcct ccttttaagg
     4141 gctcacttga ctacaattca acctctaatg accttcttgc aaccgtaaaa accaaaaaaa
     4201 cagaattact ctttctttct cttttcttgc gaactttaaa accaggtgga cgagcagcag
     4261 ttatcgtacc tgatggtgtc ctttttggtt cgtctaaagc tcataaagga attcgtcagg
     4321 aaattgtaga gaatcataag cttgatgctg taatctcaat gcctagtggt gtgttcaagc
     4381 cttatgctgg agtttcaact gccattctca tctttacaaa aactggtaat ggtggtactg
     4441 acaaagtctg gttttacgat atgaaagcgg atggtttaag tttggatgat aagcgacaac
     4501 cgattagcga caatgatatt ccagatatta tcgaacgctt tcatcatctt gaaaaagaag
     4561 cagaacgtca gagaacggat caatctttct ttgttccagt tgctgagata aaggaaaatg
     4621 attatgattt gtctatcaat aaatataaag agattgagta tgaaaaagtt gagtatgaac
     4681 caacagaggt catcttaaag aaaatcaatg atttagaaaa agaaattcaa gctggcttgg
     4741 ctgaattaga aaaattactc aagtagagag gtggctgtat gaaaaaagtg aagttggggg
     4801 aagtcttatc tctaaaaaaa ggcaagaaag ccactgtact tgctgaacaa acaactctaa
     4861 gccaacgtta tattcaaata gatgatttaa gaaataataa taatttaaaa ttcactgaaa
     4921 gtttaaatat gactgaagca ctcccagatg atattctgat agcatgggat ggagctaatg
     4981 caggaacagt tggttatgga ttatcgggag ctgttggtag tacaattacg gtcttaaaaa
     5041 agaatgagcg atacaaagaa aaaattatat cagattactt gggagtcttt ttggaaagta
     5101 aatcgcagta tttacgagaa cattcaacag gtgcaacaat tcctcattta aacaagaata
     5161 tattacttga tttacaatta gaattgctag gtatcgaaga acaagagaac attatctgta
     5221 ttcttaatac gattaaaagg cttattacta aaagaaaatt gcagttagat gaactaaact
     5281 tgctcgt
//
LOCUS       DAVMHG010000128         5243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMHG010000128 DAVMHG010000000
VERSION     DAVMHG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.130"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..488
                     /locus_tag="VYR08_001788"
                     /pseudo
     CDS             1..488
                     /locus_tag="VYR08_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            416..868
                     /locus_tag="VYR08_001789"
     CDS             416..868
                     /locus_tag="VYR08_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467275.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNTTIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1700..2056
                     /locus_tag="VYR08_001790"
     CDS             1700..2056
                     /locus_tag="VYR08_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467276.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            2061..2426
                     /locus_tag="VYR08_001791"
     CDS             2061..2426
                     /locus_tag="VYR08_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV3467277.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            2413..4236
                     /locus_tag="VYR08_001792"
     CDS             2413..4236
                     /locus_tag="VYR08_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV3467278.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            4350..5216
                     /locus_tag="VYR08_001793"
     CDS             4350..5216
                     /locus_tag="VYR08_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467279.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
BASE COUNT         1871 a          787 c         1066 g         1519 t
ORIGIN      
        1 agcagaggca atcataaaaa atggaataga aatagttgta gtaactgatc ataatactac
       61 caaaggtatt aaaaagttac aaatggcagt ctcaatcata atgaaaaatt atccgattta
      121 tgatatacat cctcatattt tacatggagt agaaattagt gcagcagata aattgcatat
      181 tgtatgtata tatgattatg aacaagaatc atgggttaat caatggttaa gtgaaaatat
      241 tataagtgag aaagatggaa gttatcaaca ttcactgact ataatgaagg atttcaataa
      301 tcaaaaaata gttaactata ttgctcattt caatagttat gacattttga aaaaaggttc
      361 tcacttatta ggtgcatata aacgaaaaat tttttctaaa gaaaatacac gatttttgga
      421 gtttaatatt aactcgaaag aatcttcgca acaacttgat attctctata aagaagttgg
      481 tgtattaagt ttgggacaaa aagttgtagc catgcttgat tttttattag catatagtga
      541 ttattctaaa gacttcagac cattgattat tgatcagcct gaagacaatc tagacaatcg
      601 ttatatttac aggcatttag ttcagcagtt tagagatgtg aaagctcaac gtcaaattat
      661 tttagcaaca cataatacta caattgtaac aaattctatg acagatcaag ttgttattat
      721 ggagtcagat ggagttaacg gatggattga atcacaggga tatgttagtg aaaaatatat
      781 aaaaaatcat atcatcaatc aattagaggg aggaaaagat tccttcaagc ataaaatgtc
      841 tatatatgag acggctttat cagagtagag tcagaaaaag taggttagaa atttagccta
      901 cttttttctt tgtccgacag gcatagtgta catctgaggt ccaagtcctc tgtggatatt
      961 tgctgcagat gaaaccaata gcgactccta agcctgaata tcgtgaggta ggggggatag
     1021 gaaggaatta gcgaaatcaa ggttctacaa acagaatcgt gacttgaagc catatatagc
     1081 ggatgaggaa ctctaaaatc caaataggtg tcgtaaccta tatacgtaaa ttacgagagt
     1141 aaactaggaa agatgtacgg cttattccgt gagcgtttag gacgtagtac aacgaatcat
     1201 gggagtcagc tgaacacata gtattgaaga aatttctgta atggaaatgg agcgaagaag
     1261 tgaacaatta aatgaatacc tctctaatta aatttgtcaa ttctaattcc tggtatgaaa
     1321 agacagtgac ctgaaaatgt aaacgatggg agctgatcat aaatatagga cggtacatgc
     1381 agtggtgtta gagattagtc cttacttgat ttgtgataac ttccccaaat ttcttctgct
     1441 atacttttct caacttttaa aaatccaact aagaatttta cctgggggtt tgggggcgga
     1501 gccactaagt tatcttatcg ttagctgtca aaactggtag gttttgatag gctggcgata
     1561 tgatttttgg gatattgtgg acacaatatc tgagctcgca aagccttaca agaatgaaaa
     1621 tcagttgttg gaaaagtgta ctgacattgt atggtagctc acattgtcag tacaagtatt
     1681 ttggaaagga agtagcagta tgaaacgaga tgtgcgtgat attcggaaac aatttcgttt
     1741 aacagaagca gaagaaaagc aaattctagc tttgatgaga gagcggggag agactaattt
     1801 ctctgatttt cttcgtaaaa gtttactttc ctctgattta caaaaacaga tggagacatg
     1861 gtttgccctc tggcaatccc aaaaactaga acaaatcagt cgtgacgttc atgaagtttt
     1921 aatcttggca cagtcagaac gtcaagtcac ccaagagcat gtatctattc tcttaacgtg
     1981 cgtgcaggaa ttgattcaag aggttgcaaa caccataccc ctcagtaaag aatttcgtga
     2041 gaagtacatg aggtaagcac atggaacatc gttaccgaac caatctcaag aaagtgtttt
     2101 tgtctgatag tgagttgaac caactaaata taaatatcga tcaaagtggt tgtaaatcct
     2161 tttctgaata tgcgagacga actctactcg atcctggtat gaattttatc acgattgaca
     2221 caaacggtta ccaagattta gtgtttgagt taaagaggat tggcaataat atcaaccaga
     2281 ttgctcgaag tgttaatcaa tctcagttaa tttctggtga agaattgcag gagttgaaaa
     2341 aaggaattgg tgaattgata aaagaagttg ataaggaatt taatctgcaa gcgcagaagc
     2401 taaaggagtt ccatggtcat cactaaacac tttgccattc acggaaagag ttaccgcaga
     2461 aagcttatca agtacattct caatcctgag aaaaccaata atcttgcctt ggtgtcggac
     2521 tatggcatga agaattttct ggactttcct agctatgagg aaatggtgca gatgtatcat
     2581 gaaaatttca tcagcaacga tacgctttac gattttcgcc acgacaggat ggaagaaaat
     2641 caacgaaaaa tacacgctca ccacatcatt cagtctttct caccagagga tcatctcact
     2701 cctgaacaaa tcaatcggat aggttatgag actgtgaagg aattaactgg tggcaaattt
     2761 cgttttatcg ttgcgaccca tgttgataaa gaccacctgc acaatcacat cattatcaat
     2821 tcagtagata gcaattctga caaaaagctc aagtgggact acaaggtgga gcgaaatctt
     2881 cgcatgattt ctgaccgttt ttctaaaatc gcaggtgcta aaatcattga gaaccgctat
     2941 tctcaccagc ggtatgaagt ctatcgtaag actaatcaca agtatgaact caagcagcga
     3001 ctctattttt tgatggaaca ttctagggac tttgaggatt tcaaaaagaa tgctccgcta
     3061 ctacatgtgg agatggattt ccgtcacaag catgccacct tttttattac ggactcaact
     3121 atgaaacagg tggtgcgtgg caagcaactc aatcgcaagc agccttacac agaagaattt
     3181 tttaagaact actttgccaa aagagaaata gaaagtctca tggaattttt attgctgaaa
     3241 gttgagaata tggatgattt acttcagaaa gcaaaacttt ttggactaac tatcaatcct
     3301 aaacaaaagc atgtttcttt tcaatttgca ggagtggagg taaaggagac agagctagac
     3361 cagaaaaatc tttatgatgt agagtttttc caagattatt ttaaaaatag aaaagattgg
     3421 caagctccag aaactgagga tttcgttcaa ctttatcaag aagaaaagtt atccaaagaa
     3481 aaagaacttc caagcgatga gaagttctgg gagtcctatc aagagttcaa gagtaacaga
     3541 gatgccgttc atgaatttga ggtggagttg tcactcaatc aaattgaaaa agtagtggat
     3601 gatggaattt acgtcaaggt caagtttggt attcgtcagg agggacttat ctttgtgccg
     3661 aacatgcagc ttgatatgga agaggataag gtgaaggttt tcatcaggga aaccagctcc
     3721 tactatgtct accacaaaga cgctgccgag aaaaattgtt atatgaaagg tcgaacctta
     3781 attagacagt tcagctatga aaatcaaacc attccattac gcagaaaagc gacagtcgat
     3841 atgattaaag agaagattgc ggaagtggat gctttgattg aactggaagt agaaaatcaa
     3901 tcttatgtca cgattaaaga tgagttagtg catgaactag cagcgtctga attgagaatc
     3961 aatgagttgc aagaacgaat gtcaaccttg aatcaagtag cagaatatct actggcttca
     4021 gttgaaagta agcaagaaat gaaattaaat ctttcaaaac tgaatataac tgagaatatc
     4081 agtgctaata ttgttgagaa aaaattgaag agcctgggga atcaactgga attggaaagg
     4141 ggcaggtatg aaaagatggt agtcaggcta gataaattta taaatcgctt aaatacagga
     4201 ctttcaaaag gagatgggat agattttcaa aaataggaat ctagccaata attatataca
     4261 atgtgtatct tttgtaaaat atatttgaaa tttagattta atcatgttac aattaacatt
     4321 gaaatcaaat agaaaaggaa gatgcaaacg tgggtacact attagcaaca agattaaaaa
     4381 atagacgaaa agaattaaaa atgtctcagc gagaattagc tgagggtatc tgtaaacagg
     4441 ggcaaattag tcgattagag agtggagagt tcacccctgg agcggatttt ttacatgccc
     4501 ttgctaagaa attaaaagtt agtatggact acttttttga tgagcaggtt gtagaggaag
     4561 tcaatgagtt atcagagttt aagaagttag cacagacatt tatcacaaat cgtaattatg
     4621 aatctttaaa atatatatat gaattggaaa atgtaaaagc ccatcgctta tctctagcag
     4681 ataaatttta catggaatgg ataaagtctc ttatagattt ttatttttat ggacagaaag
     4741 aagaggcaat tacacgatta gagaatatgc tacctcagtt aaatgtatct gatttgaact
     4801 accttcaagt ttcaaacact ctattcaatt tttattatga tattgaagat ttagaacgtt
     4861 ttaatgagat tagagacaag ctagagcatc aagtgaatca gcttaattta aacacaattg
     4921 aagagttaga actatcaatt aagttcaatt ataacgtttg tcgataccta tggttacaga
     4981 aaaatattga agaagctatt acgaaaatca cagctacgat aaagcaatgt aaggagtaca
     5041 ggacaactta tctattagct gatttgtatt tattgatggg aagtgtaagt gaaaattttt
     5101 cttctaaaag ttcagtaaaa gaatattttg agactgctca ttttctctac aaacttgagg
     5161 aaaacatgtc aatggctttg aaagtcgaac attattttgc agatataaca gaataaggaa
     5221 tacggtgttc tagtgagcat cgt
//
LOCUS       DAVMHG010000129         5180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMHG010000129 DAVMHG010000000
VERSION     DAVMHG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.131"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..644)
                     /locus_tag="VYR08_001794"
     CDS             complement(<1..644)
                     /locus_tag="VYR08_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467280.1"
                     /translation="MVEIKSLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKG"
     gene            complement(632..1189)
                     /locus_tag="VYR08_001795"
     CDS             complement(632..1189)
                     /locus_tag="VYR08_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV3467281.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSNVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            complement(1090..1719)
                     /locus_tag="VYR08_001796"
     CDS             complement(1090..1719)
                     /locus_tag="VYR08_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV3467282.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            complement(1730..3451)
                     /locus_tag="VYR08_001797"
     CDS             complement(1730..3451)
                     /locus_tag="VYR08_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467283.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQYLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            complement(3435..>5180)
                     /locus_tag="VYR08_001798"
     CDS             complement(3435..>5180)
                     /locus_tag="VYR08_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467284.1"
                     /translation="YKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLDKLI
                     INSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKASFR
                     FFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILL
                     ALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFY
                     KAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIM
                     ILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIE
                     FENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSIKIG
                     GIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLI
                     LDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQ
                     KAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEM
                     FNSANEWRVSNERVL"
BASE COUNT         1667 a          981 c          676 g         1856 t
ORIGIN      
        1 cctttatcta ttaaaaatac acggtcaact atgtccatca aataataaat tctatgctct
       61 gcaacaatta tgcttatacc tttctctttt aatatcttta gcatctttgc caaaacactt
      121 atgcttttaa tatctaaatt cgatgaaggc tcatccataa ctataatctt tgtacctgct
      181 atataagaag ctgcaatgca aagaatttgt ttttcaccgc cggatagatt aaatatattt
      241 ctgttcaaaa gattttttat cgggaatatc ttaagcatat ccttcaaacg cctgtccatc
      301 tcttctcttg caagaccgat attttccaaa taaaataata attctaatgt cgtattgaca
      361 ttaaaaaaat atgtcttagg attttgaaaa acagttgaaa caagcattga gatttgataa
      421 agttccaaat tttttatatt cttaccatca ataatgattt cgccctttgt ctttgcgttg
      481 tcatacctta cagctagtcc attgattgaa tttattaaag atgacttacc atttccactt
      541 tttcctgtaa atagcacgca ctctccctcg gctatggata gtgatatatc atctaatata
      601 tgctcttcac cataatcaag acttaaactt tttatttcaa ccatattaag cctcccacaa
      661 taagtccagc aaccgctaaa acataaacaa aatcaattag ctgtatcttt acgcgaatgt
      721 atctggtctt ggcaattgga ttttctattg cctttgtttc tgccgctttt gcaatgtcat
      781 ctgatatatt agatgatata atgagtagtg gcacagaaac atattcaaga tatttgactg
      841 ggtttttaaa atttatccct cttactctca tagccatttt gatgtttttc ttctcctcct
      901 taaaagatgg gaagaatcta aacataaccg caataggtat ggatacattc tttgaaatct
      961 taagcttatc catcgatgaa attattgcgc ctacattcga ggtctttatc ataaagcttg
     1021 ctgccataaa tggtaaaata aacattctaa taacaatagg tatactcaaa aacatcttaa
     1081 ctatagggtt taattcagat aacaccataa aattaggcaa ggagtaaagt atcccacaca
     1141 atataatcca tttgaaaagt gttttttcaa tagagccttg cctatatatg ctccaagaat
     1201 tcctcctaag aaagcaccta aggctactaa agccatgtga ggataagtta ttaacttttc
     1261 taatacatca acataatctt ttcccatagt catcaaagac cactccatat atttttcttt
     1321 tgctaaaagc atttgcatta aagagctaca tatccatgtg ctgaagattg cataagaaag
     1381 catattgtat ttaaatgaat tataattacc aatctttcta attaattctg ctattatcat
     1441 tactataagt gataaaacca caactacgta ggtatgacca gctgcaaaca tcacaagtgg
     1501 tgatatcata ccaaatataa atagtgccca tggcttttga accttagcca taaataacat
     1561 aacaacagtt cctgcaacta tggctaatac tgtcgggtat attaagaaca atattggaat
     1621 caagcccatc ataccaactc caaacatgaa ggcaaaataa attacgccaa aaacaccgat
     1681 gcttactaaa tcttttactt ttaatttttt attatccatt gtagtcctcc taataaataa
     1741 attcttctgc catttttgtc ttttctatta aatttttgta aatttttgat ttttgtaaaa
     1801 gctcttcatg cttaccttcg ctttctactc ttccgttttg aagaactact atcttgtctg
     1861 cattttctat ggatttcatt ctatgtgaaa tgattacaac agttttatct ttaactaaat
     1921 tatttaaaga ctcttgaatc tttttctcgt tgttaacatc aaggcttgct gttatctcat
     1981 ctaagatcaa tatcggcgca tcttttaaga aggctctggc tattgataat ctttgtcttt
     2041 ctcctcctga tagctcagct ccgttctcac caataactgt atcgaaacct ttatccattt
     2101 tttctataaa atctgtgcaa tttgcaagtt ttgctgctct tttaacctct tcgtcacttg
     2161 catcttgctt tccgattcta atattttcca taacgctttg attaaagaga accacatctt
     2221 ggaaaactat agagacctta tcaaaaagag attctgttga tatttctttt atatctttgc
     2281 catcgattaa gatttgtccc ttgtcataat cataaagtct tgatataagt ttcaagatag
     2341 ttgttttacc gcagccactt gcacctacca aagcagtgac ctctccctgc ttagctttaa
     2401 aacttacacc atttaaaact tttgcgtctt tattgtaggc aaactcaaca tcttttagat
     2461 caatatcaaa tttttttaag ctatagtcat cgccttcttg taaatattga ttttgaattt
     2521 cttttaatct ttctattttg ggcgataaat aaaatatttc catcaagccc tctttagatg
     2581 catctaaaga gtcttttatc ttcatagcag ctagtaaata tcctataagg tagagagaat
     2641 ttatctcttt attaataatt agatttacgc cgacaaatat cacaacagca agagatataa
     2701 agctaaatat tgaagatata gacaaagtta aaattgtagt tacttccgcc tttaagtgta
     2761 ctctctcact atcttccatt tttttatata agtcatcttt aatatccttc gataaattat
     2821 atgctttaat ctccatttgc atttcgatat tttcttgaaa gctttctgag ttttttctta
     2881 agacatcata atatctattc tgtccattaa cctgatattt tttagataaa ggtataaata
     2941 taaagcttaa aatagatgga ataattacag ctaaacccat cttgacattg cccgctagca
     3001 tcattacaga tattaatggg aaaaacagta ccatgccgcc cacctttggt attgagtggc
     3061 tcattgcatg ctctatgcct tcaatatcag ccatgattgt ttgtgaaatg tcggaaatgt
     3121 catgtttaga aaagtaagat agaggtaatt ttgataaatt ctccgctgtc cttattctta
     3181 aatctgcact ttcttgatag gttgtgttat ataatttatc gtattcgata gaaagcaaaa
     3241 tatacattgc tatcaaagtc aaaactgaga atactatata aaagccattg cttttgccca
     3301 tattttccaa aacttcctga gcaaaaatca taagtaatat ggcaggaagc atgtttatac
     3361 aataaacgaa aaatgaagcc agtgttgctt tactcaaatt tcttgctcct ccatctgtaa
     3421 gagcaaatct ttttttataa aactctttca tttgaaaccc tccattcatt cgcactgtta
     3481 aacatctctt gcaggctctt ataccttgta tcttttgaca ttaattcttt gtcagaccct
     3541 ctttctataa tttttccact atccatgaca ataatttcat caaggtcttt aattgtagat
     3601 agcctgtgtg caatcatgat aactgtttta tccttcataa gatttttaaa agctttttgc
     3661 aattcaaact cattatctgg gtcaatagat gctgatgctt catccataat aataattttg
     3721 gaatccttta aaattgctct agcaattgca attctttgtt tttctccacc ggataaataa
     3781 acaccttttg agcctatgat tgtattttct ctttctggga atttgtctaa tattaaatcg
     3841 catcctgcta attttaaggc tctcataacg tcatctttcg tcgcatcttt attagctaaa
     3901 gctacattat cataaatgct cttcttgaat aattttgaat cttgaaaaac aaaggaaatg
     3961 gctttaatta aggcttcgtc agaatattca cttattgcta tcccgcctat ctttatgctt
     4021 ccttcattaa cattgtaaaa acctgatata agttttgcta ctgttgattt gcctgatcca
     4081 gatgaaccga caagtgcgta ggactttcct tcttctaaat taaaggataa attttcaatg
     4141 acagctttat cattataagc aaagctaaca ttctcaaatt ctatattata gtttttaaaa
     4201 ttattgacat taccatgcac taatttgtct ttttgcatat cttcgtaaag cgcctctaaa
     4261 gtatctactg cataatttcc ttgagaaata tacatggagt accacatcat tctcatgaat
     4321 gaaacaaaga gaactcctga taaaaataaa atcatgataa gctcaagtaa aatcaccttt
     4381 gcgctagcta agctagtcat aaaataaact ataggaataa ttaaaattgc aatcagtcca
     4441 aaaaataacc attgatacaa aacataaggc cttttacaag atagggaata atcataagca
     4501 tactttgagt aatcttttat agccttgtaa aagcttttaa atgactctac atttgcttta
     4561 aatattttta caacttgcat tcctctcacg tactcaacag tttcagcact tagtttagat
     4621 agggattctt ggtatatctt cataaattct tgctcgccca tcatcgcccc taaaattaag
     4681 ccaccaatta tagtaagagc aagcaaaatt atgccaactc ttatacttac tataaagcca
     4741 agtgcaagta caagtacggg tgtgattatt gcctgagaac tatcgggaat catgtgtgct
     4801 accacctgat gagtttgtgc tgcattatca tctatgatct ttcttatttg accagatgga
     4861 tttaagtcaa agaacctaaa acttgctttt tccagaccat cgattcccct ttttcttaaa
     4921 tttgtttcaa gcctgaatcc caagatatgt gaaaacattc ctgagacaaa ataaaatatc
     4981 gctccacttg ttagtgtaat aacagatttt aatgctatac tctctgcacc ggataaattt
     5041 gaattaatta ttaacttatc tagaaatttg tagattaaat aatatccata tactgtaagt
     5101 agagcagata tagcagaaaa aattatggcc aaaaccccaa gatatttctt atcttggaca
     5161 taagcaaata gttttttgta
//
LOCUS       DAVMHG010000130         5180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMHG010000130 DAVMHG010000000
VERSION     DAVMHG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.132"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(32..2272)
                     /locus_tag="VYR08_001799"
     CDS             complement(32..2272)
                     /locus_tag="VYR08_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV3467285.1"
                     /translation="MLQWIKNFPIPLIYMSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFALSFLYPVIQLNFIFEWLEGMIRLVSKVASRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDIGQGESIFLRDMTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQVTQTKVRNVTAGENLPIFGSQLEVLSPRK
                     IGDGDHEDSLVLYGKLLDKYFLFTGNLEEKGERDLLKHYPDLKVNVLKASQQGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(2256..2897)
                     /locus_tag="VYR08_001800"
     CDS             complement(2256..2897)
                     /locus_tag="VYR08_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV3467286.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVNKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQKAGG
                     LTEQADSKSLNLAQKVSDEALVYVPTKGEESASQQAGSGAPSSTSKDKKVNLNKASLE
                     ELKQVKGLGGKRAQDIIDHRETNGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(2965..3534)
                     /locus_tag="VYR08_001801"
     CDS             complement(2965..3534)
                     /locus_tag="VYR08_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467287.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     IFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            3711..4136
                     /locus_tag="VYR08_001802"
     CDS             3711..4136
                     /locus_tag="VYR08_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV3467288.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            4137..4550
                     /locus_tag="VYR08_001803"
     CDS             4137..4550
                     /locus_tag="VYR08_001803"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV3467289.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            4496..4822
                     /locus_tag="VYR08_001804"
     CDS             4496..4822
                     /locus_tag="VYR08_001804"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467290.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
BASE COUNT         1459 a         1166 c         1009 g         1546 t
ORIGIN      
        1 ttatttcact aatctacaac atttatcctt cctatcgaac actttcgatt ttccaactat
       61 ccaacccctt aaaacgtata gctccttgct ggtcagttcg ataaactttg ctattgatac
      121 cttccagtcg tgtcaatgtt tcctgatggg ggagtttcat tcgattgctc tttccaactg
      181 agataagagt aagctctggt ttgagttttt ctagaaaggc tggacttgat gattttttat
      241 ttccttgttg gctagctttc aaaacattta ctttcaagtc tggatagtgc ttcagcaagt
      301 ccctctctcc tttctcctcc aaatttcctg tgaagagaaa gtatttatcc aagagtttcc
      361 cgtatagaac aagggaatct tcatgatctc catctccaat tttccttgga gataggactt
      421 ctaactgact tccaaaaatt ggcaagttct ctcctgctgt cacattgcgc accttggttt
      481 gagttacctg tagttctgcc acaaattcct tctgtttcag actgtctttt gatactagaa
      541 tctcccctac atggaaagcc ttggtcacct ctgacaaatc tccaacatgc tccttgtccg
      601 tgttagtcaa aattagctgg tcaatcttag ctactcctcg acttttgaga tagggaatca
      661 aggttcgctg ggcattgctg gtcgtcatct tttcttgcca tttttcgatt ttcttataag
      721 attctgcctt gccaccgaca tctatgagaa tggttttacc agtcatatcc cgtaggaaaa
      781 tactttctcc ttgcccaata tccagcatgg tgatttcatt ttccagtgga tgcttggtca
      841 ggagaaagag ccctgtaatc aataagcaca ataccgttag ctttttaata ttttttctca
      901 aatcatatac taaagccaag gaaattaaca acaagattaa aagccatgca ttgggttgtc
      961 caaagactag aggcctactt gccacctttg ataccaagcg aatcattccc tccaaccatt
     1021 caaagataaa gttcagctga atgactggat atagaaagga aagggcaaat aagatagaca
     1081 agagcggtaa gaagaccaag tcaaaaagaa aggaaaagac aaaggtcaag aggatggacc
     1141 aaggttgaaa ttccgcaaaa tagaaggaca gaatgggcaa tatgcccaag gagatgacta
     1201 gactttcact agcaacagcc ttgagcccct ccccttcttt gctggtcatg gtcaggataa
     1261 aagcataagc acaggacaag actcctcctg ctgtcaagaa aaagtttggc atgacaataa
     1321 agaggacaag caccgtcaag gcaaaattat ccaagccctt aaccccatgt tgagccagta
     1381 gcttttgcaa gagactgcga ataaccgatg ctgaaaatcc agttagtccc gcatagataa
     1441 gggaaaaggg ataagtcagc cacttcaact tttcttgtgt caagcccaat cgtaaaagta
     1501 gtttcttaaa tccgtccatg aaaaacccta cctgcatacc tgacaaggca aaaaggtgaa
     1561 taattcctaa actggaataa agctcattca tctcctcaaa gtcggtgtcc agatgtccta
     1621 gcaagagtcc tgtcatgtaa ttgcgcatag ggtctgggaa gtgcatctta atccaaacca
     1681 cagcctttcg acgtaaactg gacaggtttt cacctatatc ccaactgcca accttttgga
     1741 gtgactggat tcttttaata ttgagtgtct ggtaaattcc ctgagtcttc agatagcctt
     1801 ggtagtcaaa gccaccaaaa tttctctgcc cttctggctc cgaaagcttc ccttctagtc
     1861 ctatctcatg aaggtcagtt aaagcttgaa aggcttcttt ctcctcctcg gactggagtt
     1921 tataatagac ttggaaagca cgaccatcag acttaccacg aaaggacaga ctgtcaccgt
     1981 taaccttaat agtgtctggt aaaatccgta ccctttcaac agaatccacc aaattttgac
     2041 tcacttgact ctgttgccaa gtttgaaaca gaaaccagaa gccaaagact ccacaaatca
     2101 ctagaacttt acctgctgat ttccaaggaa attggataaa gaggcagact agcagaaaaa
     2161 caaaacccaa caaagcaaaa taggatactg agaaaatggc gtagtaaagc caaagtaaca
     2221 gaaagctcat gtaaatcagg ggaatgggga aatttttaat ccactgtaac ataatcttta
     2281 agcttttcta tggtctttgc accaattcca gataccttct tgagttcatc aaccgactta
     2341 aatttcccat ttgtttcacg atggtcgata atgtcttgag ctcgttttcc tcccagaccc
     2401 ttgacctgct tgagttcttc cagactggcc ttgttgagat tgaccttctt gtccttgctt
     2461 gttgaagaag gcgctccaga acctgcctgt tgactagctg attcttctcc cttagtggga
     2521 acgtaaacca gagcctcgtc actaactttc tgagctagat tgagcgactt actatctgct
     2581 tgttctgtca agccacctgc tttctgcacg gcatcattga ctctactacc taccggcaaa
     2641 tcgtaaatac ctggagattt gacagcacct ttgacatcta ctgtgattag atcttgttca
     2701 aggggttctt ccttgttcac ttccttttcg gtcgatgaat ccttggaaac agctgcaact
     2761 tctgcctgca aattcgtttc cttgacaggt gtttgtggag ctggctttag caggaaaaat
     2821 ccgcctacaa gcaagcccag accagtacag atgacgatga ttttatactc tttgattttc
     2881 tcgataattg cttccatatt ttctcctctc ttagattatt cgtaagagga agaaaaaaca
     2941 gtcgaaaaat ttcttttcaa ctgcttattt atttgcaaaa tagtcttcct tggtcaagac
     3001 ataatgaact cttgtcacga ttcggccttt ttcatgctgg tccatacaag catatggttc
     3061 tgcatgggaa aaacgcatgc ctgatttctc catgaccttt cctgacgcag gattgtcctt
     3121 atcgtgaagg gcagtcaact tattcatccc tatcttctca aaagctagct caatcacagc
     3181 acgattggct tctgtcgtta atccttgatt ccaatacttt ttattgataa tgtagccaat
     3241 agctgccttc ttaagaacag aatcaatctt gtgcaagtca atggttccaa taaactgacc
     3301 attgcttttt agttctattc cccaacgtcc caagggatta gccaaataga actgagcaat
     3361 gttattcttg gtttcttcca agctttgatt ggttggaaaa atgtaacgtg tattaccctt
     3421 gtccgaggca tagtcaaaca ttgcttccgc atcatccaaa gttacaggtc taagcaataa
     3481 acgctccgtt tctatagacg gatactgggc aagttttaca aaaattgatt ccatactttt
     3541 ccaaccaatc aaagcttgat tttcactagt ctagcataaa aaatttggat gacaagtttt
     3601 tcttgatttt tgaagcggtt tcatatacaa tgaaaccgtc acatttttga tttatgaact
     3661 ggaatgtgac aaccaaaaca agaccgtcaa ttgaaaggta ggttatctca atgttaatcg
     3721 gaatcccaaa agaaattaaa aataacgaaa accgtgtcgc cctcacacct gcaggtgttc
     3781 atagcttagt tagtcgtggt catcgtgtcc ttatcgaaac aaatgctggt ctcggttctg
     3841 gctttactga tgctgactat caaaagcaag gagctgagat tgtcgctact gctggtgaag
     3901 cttgggcagc agagttggtt gtgaaagtaa aagaaccttt aagttctgaa tacggttact
     3961 tgcgcgacga tcttcttctc ttcacctact tgcacatggc cgctgctcca gaattagcag
     4021 atgctatgtt agcagcaaaa acaacaggaa ttgcctatga aactgttcgt gacaatcaag
     4081 gacaactacc gctcctcgtt cctatgagtg aggttgcagg tcgtatggct gtttaaatcg
     4141 gagctcactt ccttactaag caagctggtg gctctggtgt tctacctggt ggtgtaccag
     4201 gtgttccaaa aggaaaagta actatcatcg gtgatggtgt cgtcggtaca catgctgccc
     4261 gcatcgccct tggtcttggt gctcaagtga ctattttaga tattagtgcc aagcgtctct
     4321 cagttctaga agaagtcttt ggaagtcaaa ttcaaactct tatatctaat tcattcaaca
     4381 ttgaagcaag tgtgagagat gctgatgtgg tgattggagc cattctcatc cctggtgcaa
     4441 aagcaccgga attggtgaca gatgagatgg tcaaacaaat gcgtccaggc tctgtatcgt
     4501 tgacgttgct gttgaccaag gtggccttat cgaaacagct gaccgtgtga caacgcacga
     4561 tgaacccgtc tatgaaaaac acggtgttct ccactatgcc gttgccaata tccctggtgc
     4621 ggttgcccgc acttcaacca tcgccctaac caatgtcact cttccttata tcgaagcttt
     4681 ggctggcaaa ggattcgcac aagcaatctc tgaagatgaa ggcttgcgtc aaggtgtgac
     4741 cacttatcaa ggttacttga ctagcctacc agttgctcaa ggacttaatc gtgactacac
     4801 tgatatcaat gatttagtat aaattttgaa aagaaaaggg tgacttcgca agtcctaccc
     4861 actgtgcttt gtgtcatcag ctattacatc gttggtttat tgtactggag actttgcaac
     4921 tggagacttt cgttccagac tcaattgtgc ataagaaact ctaaattgaa taaagtcaac
     4981 agagtttcgc ccaaccactt cgtcttgtaa tgacaattat ataaaccaaa ggaagctgag
     5041 ctctttagtc ccagcttctt tttatatata aaattttacc cgtgcaaaga cagggcctta
     5101 gcagacttct tttttacttc gttcaccctt gctttttctt tttaggtttg ggcgttggca
     5161 gttggttata catagctaaa
//
LOCUS       DAVMHG010000131         4988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMHG010000131 DAVMHG010000000
VERSION     DAVMHG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.133"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..566
                     /locus_tag="VYR08_001805"
     CDS             <1..566
                     /locus_tag="VYR08_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV3467291.1"
                     /translation="IWNEYETFNNIRVLDVGADLAYFKHLKLLSNELDEVLSQVIVEM
                     IDFYNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLD
                     SIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKN
                     LRMIISALQNTIPFESIKERIRPHYGS"
     gene            556..876
                     /locus_tag="VYR08_001806"
     CDS             556..876
                     /locus_tag="VYR08_001806"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV3467292.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            939..2714
                     /locus_tag="VYR08_001807"
     CDS             939..2714
                     /locus_tag="VYR08_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV3467293.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVSAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            2715..4100
                     /locus_tag="VYR08_001808"
     CDS             2715..4100
                     /locus_tag="VYR08_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV3467294.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4123..4734
                     /locus_tag="VYR08_001809"
     CDS             4123..4734
                     /locus_tag="VYR08_001809"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV3467295.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1641 a          714 c         1046 g         1587 t
ORIGIN      
        1 caatttggaa tgagtatgaa acttttaata atattcgtgt acttgatgtc ggagctgatc
       61 tagcatattt taaacatctg aaacttttat ctaatgagtt agatgaggta ctgtctcagg
      121 ttattgtcga aatgattgac ttttataata ttattactgt aaaacgtggt ttatctcaaa
      181 ataagagtca tggggatatt ttacaattac tttcagatga aggaagtatt tctgctaaag
      241 aatttatata cattgtagaa aatcaagaaa tatttgtgtg gttcaataaa ataaatccaa
      301 gcttagattc aatcttttca acttatgaat tgaagatgca ggacgcaaca atttcatctt
      361 ctgagttaga atttttatgt gatttactat tgtataaaac tttagatcaa ggaaggtaca
      421 atgtagaggg gccgttagtt cttgctagat atttattggg atgtgagttt gaagtaaaga
      481 atctcagaat gatcatatca gctcttcaaa atacaattcc ctttgaatca ataaaagaaa
      541 ggatacgccc acattatgga agctaataag tataaaattg gcataattgg tagccgtgat
      601 attattttac catttagcat gattgggttt gatatatttc ctgcctacca agaacaagaa
      661 gctataaata cactaagaaa attagctcaa tctgattatg gtgtcattta tatcactgaa
      721 gacattgctt caatgatatt agatacaatt cgccattatg attcccaagt tgtgcctgct
      781 attattttat taccgactca taaacaaggt ttaaatttag gattaaaacg tatagaggat
      841 aatgtagaga aagcagtagg acacaatatt ttataataat gtacaaaatt gtctgtaata
      901 ttattctata atttttggac ttagtaagga gaataacttt gactcaaggg aagattataa
      961 aagtatcggg acctctagtt attgcatcag gtatgcagga ggctaatatt caagatattt
     1021 gccgtgtagg taagctaggg ttaatcggtg aaattattga aatgagaaga gatcaggcat
     1081 ctatccaagt ctatgaagaa acatctggtc ttggtccggg agaacctgtt gttacaactg
     1141 gagaacctct ctcggttgaa ttagggccag gattgatttc tcaaatgttt gatggcatac
     1201 aacgcccatt agatcgattt aaattggcta ctcataatga ttttctagtt cgtggggtag
     1261 aagttccaag tttggataga gatattaagt ggcattttga ttccactata gcaattggtc
     1321 aaaaagtgag tacgggtgat attcttggaa ctgtcaagga aaccgaggta gttaatcata
     1381 aaattatggt tccttatgga gtatctggag aagtcgtttc tattgcatct ggcgatttta
     1441 caattgatga agttgtatat gaaataaaaa aattggacgg tagtttctat aaaggaacgc
     1501 ttatgcaaaa atggcctgtc cgcaaggcgc gtcctgtttc taaacgttta attccagaag
     1561 aaccattaat cacaggtcaa cgagttattg atgcattctt tccagtaacc aaagggggag
     1621 ctgcagcagt tcctggaccg tttggagcag gaaagacagt tgtacaacac caagtagcta
     1681 aatttgccaa tgttgatatt gttatttatg tcggttgtgg agaacgtgga aatgaaatga
     1741 cggatgtact gaatgagttt cctgagttga ttgaccctaa taccggacaa tcaattatgc
     1801 aacggacagt tctgattgct aatacttcaa atatgcctgt ttctgctcgt gaggcttcaa
     1861 tttatacagg aattaccatg gctgagtatt ttcgtgatat gggctactct gtcgccatta
     1921 tggctgattc aacttcacgt tgggcagaag cgctacgtga aatgtcagga cgtctagaag
     1981 aaatgcctgg tgatgagggt tatcctgctt atctgggaag tcgtatcgct gaatattatg
     2041 aaagagcagg acgttctcag gttctagggc ttccagaacg tgaaggaacg attactgcta
     2101 ttggagctgt atcgccacct ggtggagata tttcagaacc agttactcaa aacactttac
     2161 ggattgtgaa agttttttgg gggcttgatg ctccgttggc acagcgacgt cattttcctg
     2221 caattaactg gcttacatct tattcactat ataaagacag tgtgggcact tatatagatg
     2281 gtaaagagaa gacagattgg aatagtaaaa taactcgtgc gatgaactac ttacaacggg
     2341 aatctagttt agaggaaatt gttcgtcttg ttggaattga ttctctgtct gataatgaac
     2401 gactaacgat ggaaattgct aaacaaattc gagaagatta tttgcaacag aacgcttttg
     2461 attcggtaga tacattcact tcgtttgcaa aacaagaagc aatgctaagt aatattctca
     2521 cttttgctga tcaggcaaat catgctttag agttgggttc ttactttaca gagattatgg
     2581 aaggtaccgt ggcagttcga gaccgtatgg cgagaagtaa atatgtttca gaagatagat
     2641 tagatgaaat caaaattata tcaaatgaga ttacacatca aattcatttg atattagaaa
     2701 caggaggtct ataaatgagt gttataaaag aatacagaac tgctagtgaa gttgttgggc
     2761 ctcttatgat tgttgaacaa gtaaataatg tgtcttacaa tgagttagtt gaaattcaac
     2821 ttcataatgg agaaattcgt cgtggacaag ttttagagat ccacgaagat aaagcaatgg
     2881 ttcagctttt tgaaggatct agtggaataa atttagaaaa gtctaaaatt cgttttgctg
     2941 gtcatgcatt agaattggct gtatctgagg atatggttgg tcgtattttt aatgggatgg
     3001 gaaaaccaat tgatggtgga ccagatttaa ttccagagaa atatttagat attgatggtc
     3061 aagctattaa tcctgtatct agagattatc cagatgaatt tattcagaca gggatctcct
     3121 ctattgatca tttgaatact cttgtacgtg gtcaaaaatt accagtattt tcaggttcgg
     3181 gcttacctca taatgaatta gctgctcaga tagcaagaca agcgactgtt ttaaattctg
     3241 atgaaaattt tgcggttgta tttgcagcaa tgggtattac ttttgaagaa gctgagtttt
     3301 ttatggaaga actcagaaaa acaggagcga tcgatcgttc ggttttattt atgaacttgg
     3361 caaatgatcc tgcaattgag cgtattgcaa ctccccgcat tgctttaact gcggcagagt
     3421 atctagcttt tgaaaaagat atgcacgttc tagttatcat gacggatatg actaactatt
     3481 gtgaagcgtt acgtgaagtc tcggcagctc gccgtgaagt tccagggaga cgaggctatc
     3541 cgggatattt atatacaaat ttatcaactc tatacgaaag ggctggtcgc ttagttggta
     3601 aaaaaggttc ggtgacacag attcctattt taacaatgcc agaagatgac ataacacatc
     3661 caattcctga tttaactgga tacattactg aagggcaaat tattttgtcg catgagttgt
     3721 ataatcaagg ttatcgtcca ccaatcaatg ttttaccttc tctctctcga ttaaaagata
     3781 agggatctgg agaaggtaaa actcgtggag atcatgctcc aactatgaat caactgtttg
     3841 cagcctatgc ccaagggaaa aaggttgaag agttagcagt agtattagga gaatcggctt
     3901 tatctgatgt agataaattg tatgtgaggt ttacaaagcg ttttgaagaa gagtacataa
     3961 accaaggatt ttataaaaat cgaaatatag aagatacgtt gaatcttggg tgggaattac
     4021 tatcaattct tcctagaaca gagttaaaac gtatcaaaga tgatttgctt gataaatact
     4081 tacctttggt agaagtttaa tccggaaatg gagtgattat ctatggtacg tttgaatgta
     4141 aaaccaactc gtatggaatt gaataactta aaggaacgtt tgacaacagc tgaacgtgga
     4201 cataagttat taaaggataa aagagatgaa ttgatgaggc gatttatttc tttgattcgt
     4261 gagaataatc aacttcggaa agaagtggaa agttatctaa ttgataatct aaaatccttt
     4321 gcagttgcta aatcattaaa gaattctcaa atggtggagg aattattttc aattccatcg
     4381 aaagaaattg aattatttgt tgagaaagaa aatatcatga gtgtaacagt tcctagaatg
     4441 catatgaata ttacttctca aaatgagaac agtgaataca gctatttatc ttctaatagt
     4501 gaaatggatg atgtatttgc tacaatgaat agtttaattt ataaattact aagactggca
     4561 gaagttgaaa aaacgtgtca gttaatggct gatgaaatag aaaaaacacg tagacgtgta
     4621 aatggtttag aatactcgat tattccaaac ttgtcggaaa ctattcatta tatagaattg
     4681 aaactagagg aggcagaaag agccaattta gttcgtatta tgaaagtgaa gtagatcctt
     4741 tatttagatt attaattaga tgaacaaata tcagcttgga taaggcttta agcctttcta
     4801 agcttttttt attgacagta tcaggatatc tttttcaaaa ttttggtttg ttagataatg
     4861 aaaatgtttc tactaatcta gatttaggat tagtaaatcg taaatgtaat tatatagaaa
     4921 gtaagcgcgt cataacaagg tatctatcat tcatggagct cctcctgtat actattagta
     4981 aagtaaaa
//
LOCUS       DAVMHG010000132         4825 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMHG010000132 DAVMHG010000000
VERSION     DAVMHG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.134"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..2274
                     /locus_tag="VYR08_001810"
     CDS             1..2274
                     /locus_tag="VYR08_001810"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV3467296.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2291..2761
                     /locus_tag="VYR08_001811"
     CDS             2291..2761
                     /locus_tag="VYR08_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV3467297.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3267..>4825)
                     /locus_tag="VYR08_001812"
     CDS             complement(3267..>4825)
                     /locus_tag="VYR08_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV3467298.1"
                     /translation="ELMTRGIQTASGDEIGKTIIFAKNHDHAEYIRGIFNNRYPEKGS
                     DYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGIDVPEVVNLVFFKKVRSKT
                     KFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRADPRDGEGRHIVSLTQRLF
                     NIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLNELDFRVRMVLDTVYSYRK
                     LESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWLLHIQLGQLTAKSSTVHIS
                     QVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDK
                     TDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKK
                     LTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDE
                     NLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIE
                     LINNRAGEAA"
BASE COUNT         1497 a         1062 c          823 g         1443 t
ORIGIN      
        1 atgcgtttta atcaatatag ttatatcaat tttccaaaag aaaatgtctt atctgaacta
       61 aaaaaatgtg gctttgattt acaaaataca gccaatcaca aagactcact tgaaaccttt
      121 ttacgtcgtt tctttttcac ttatcaagac accaactacc cactttctat cctagcagct
      181 gataaaaaaa cagacctgct gacatttttt caatcagaag atgaactgac agcagatatt
      241 ttttatactg ttgcttttca acttttaggc ttttcttatt tggttgactt cgaagatagt
      301 gacgtttttc gtaaagaaac tggatttccc attatatatg gtgacttgat tgaaaatctc
      361 tatcagttac tcaatactcg caccaaaaaa ggaaatactc ttatcgacca acttgttagt
      421 gatggtctta ttccggagga taatgactac cactacttta acggcaagag tttggctact
      481 ttttctaacc aggatgtcat tcgtgaagtc gtttacgttg agtctcgtgt cgatactgac
      541 caaaaagggc tatcagacct agtaaaggtt agcattattc gccctcgttt tgatggaaaa
      601 atccctgcca tcatgacagc cagcccttat catcagggaa ccaatgacaa ggctagtgac
      661 aaggctctct acaagatgga gggcgagctt gaggttaagc ttcctcacaa gattgagcta
      721 gagaaacctc aactaaatct cgtccaaccc caaggtaaag ctgagcttat agcagaggct
      781 gaggaaaagc taactcacat caactctagc tatacactaa acgactactt ccttccgaga
      841 ggctttgcca atctctatgt ctcaggtgtt ggtaccaaag actcgacagg tttcatgact
      901 aatggagact accagcaaat cgaggcttat aaaaatgtca tcgattggct taacggtcgt
      961 tgccgtgcct ttactgatca cacgcgccag cgtcaagtca aggctgattg gtcaaacgga
     1021 aaagttgcta caacaggact ttcctatcta ggtaccatgt ccaatggtct tgcgactaca
     1081 ggagtcgatg gtttagaagt tatcattgcc gaggcaggaa tttcatcatg gtacaactac
     1141 taccgtgaaa acggtctggt gactagccca ggtggttatc caggtgagga ctttgactcc
     1201 cttgctgagt taacctattc tcgtaatctc ttagctggcg actatatccg tggcaatgaa
     1261 gctcaccaag ctgacttaga aaaagtgaaa gctcagctag accgaaaaac tggggactac
     1321 aatcagtttt ggcatgaccg caattatctg ctcaatgccc ataaagtaaa ggcagaggtt
     1381 gtctttactc atggttctca ggattggaat gtcaaaccac ttcatgttta ccagatgttc
     1441 catgctcttc ctactcatat acacaagcat ctctttttcc ataatggtgc ccatgtttac
     1501 atgaacaatt ggcaatcaat tgacttccgt gagtccataa atgccttatt aaccaagaaa
     1561 ttactaggac aggaaacaga ctttcaactt cctactgtta tctggcagga caatacagct
     1621 ccacagactt ggttatcact tgataacttc ggtgggcaag aaaactgtga aaccttctca
     1681 cttggtcaag aagagcaagc tattcaaaac cagtacccag ataaggattt tgagcgctat
     1741 ggtaagacat accaaacctt caatacagag ctctatcaag ggaaagccaa tcagattact
     1801 attaaccttc ctgtgactaa agatctccac ctaaacggtc gcgctcagct caatcttcgt
     1861 atcaaatcca gtacaaacaa ggggctctta tctgcccaac tgctagaatt tgggcaaaag
     1921 aaatacctac aaccttatcc agctatttta agtgctagaa ccattgacaa cggtcgctat
     1981 cacatgttgg aaaatctctg tgaattgcca tttagaccag aggcacaacg agtcgtgaca
     2041 aaaggttacc ttaatttaca aaatagaaat gatttactgt tagtagagga tattactgca
     2101 gatgaatgga tggacgttca atttgaactg cagccaacta tttacaagct aaaagaagga
     2161 gacactctcc gtttagtcct ctatactact gactttgaaa tcaccatccg tgacaataca
     2221 gactaccact tgactgtcga cctcgctcag tccatgctta ccttaccttg ctaaaaggag
     2281 ttacattatt atgaataaat cagaacaccg ccaccaactt atacgcgctc ttatcacaaa
     2341 aaacaagatt catacacagg ctgagttgca agcccttctt gctgagaacg acattcaagt
     2401 aacccaggca accctctcac gcgacatcaa aaatatgaac ctatcaaaag tccgcgaaga
     2461 agatagcgct tattatgttc ttaacaatgg ttccatctca aaatgggaaa aacgtctcga
     2521 actctacatg gaagacgccc ttgtctggat gcgcccagtt caacaccaag tcctactaaa
     2581 aacccttcct ggactggctc aatcctttgg ttctatcatt gatactttga gcttccctga
     2641 cgctatcgct accctttgtg gtaatgatgt ctgtcttatc atctgtgaag atgcagatac
     2701 tgctcaaaag tgctttgaag aactgaaaaa attcgcccca ccatttttct ttgaagaata
     2761 ataaatgact acaactaaca attatgcttt taaaaaacct ggtagaaagt ctttaacatc
     2821 agaaaacgca tagtatcaag tgttgaaaac ccttgatact atgcgttttg ctttatccta
     2881 ggaagattta tactcttcga aaatctcttc aatccgcgtc agcttccatc tgcaacctca
     2941 aaacagtgtt ttgagcagcc tgcagctagt ttcctagttt gctctttgat tttcattgag
     3001 tattatttca ttttctcctg caattgaatt cttgctcggc tttttgtttt ctatttcttt
     3061 aaaatcaaag tagctctttt gttaataact cgatcaacaa acatcgtggt acaagtatct
     3121 actttgaaat ttatcaacca cttaacaact gatactgtat tctaggaaaa cgatgacatt
     3181 cttcctaata aaacttctca tatatagcat aaatttctac tctttttaat tttaaatgag
     3241 tctcagcatg gcaatcactt tagaatttaa gccgcttctc cagctcgatt attgataagt
     3301 tcaatgattt gaacaatatt acgaagtact ggtagttggt gttggaagag tagttgaaca
     3361 gaaccataag atttaaacgg ctcttgcgtt aacactttcg tctctaaaaa accattttct
     3421 acaatgtagt ctacaatcaa ttttacaaat gaaatctgcc tggcattaag attctcatcc
     3481 gacaaaaatt tagaaaaaat acgattggca gactctctat ctaagccaat aatctcacga
     3541 accaatctcg gaattgcctt attttcataa tgactttggt agtctgcttt actacctaat
     3601 ttttcccaaa gcaatttttc aagtgcaagc atatcatcag atgtcaactt tttattatgg
     3661 tatagcttag aaatggactc ctcatccaga tgagttttca aataatgctc aactttttca
     3721 ttgtaagacc gaagatcgtt gacctgcaaa aatgctgtgg tctcgtgaac agtggagagt
     3781 atacgatctt caaagttaac atagtagggt ttacggtctg ttttatccaa aaactgtaat
     3841 aaatctcgaa tagcaagacg aattttttcc aaatcagaca agttaacttc tttccaaaat
     3901 tcaggctcct gtactttcct gataatttca gcctgctcaa aaacctgcgg gatattgcca
     3961 atagcagaaa gagctctagc cgtcttcatc acttgggaaa tatgaacagt ggaagattta
     4021 gctgtcagtt gccccaactg aatatgaagc aaccacaaat caaatctcct ggccatctca
     4081 tcttctttat cttcatcaaa taaaagcgga gagagatttt tttgaatggt ttcacttgta
     4141 acagcagtta gattctgcca actttccaat ttcctatagc tataaactgt atctaaaacc
     4201 atacgaaccc tgaagtccaa ctcatttaag ctctctatac gaccttgaag ttccgagaca
     4261 agctgctgac ggtatgctct cgcaaactga tcttcttggt attggagtcc ctgaagttct
     4321 cgaatcaagt ccactttgat attaaataaa cgctgagtca gcgaaacaat gtgacgaccc
     4381 tctccatctc ttggatctgc acgaaaataa tcaaaattgt ccccataatc aaataccaag
     4441 aagttttcct tatcctgctc aggtccaaat aaatctttac atagacgggt tcctcgacca
     4501 atcatctgcc aaaacttagt tttagagcgt actttcttga agaagactaa attaacaacc
     4561 tctggtacat caatacctgt atctaacata tcgacagaaa tcgcaatttg aggatacttc
     4621 tccttaattt taaaatcatc aatcaaggtc tgataatgct taatactata atcaatcacc
     4681 tgagcatagt cgctcccttt ttcaggatag cggttgttaa aaatacctct gatatattcc
     4741 gcatgatcat gatttttagc aaaaataata gttttaccaa tttcatcacc cgaggctgtc
     4801 tgaattcctc ttgtcatgag ttcat
//
LOCUS       DAVMHG010000133         4801 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMHG010000133 DAVMHG010000000
VERSION     DAVMHG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.135"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..453)
                     /locus_tag="VYR08_001813"
     CDS             complement(<1..453)
                     /locus_tag="VYR08_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEV3467299.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNL"
     gene            686..760
                     /locus_tag="VYR08_001814"
                     /pseudo
     CDS             686..760
                     /locus_tag="VYR08_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1222..1383)
                     /locus_tag="VYR08_001815"
     CDS             complement(1222..1383)
                     /locus_tag="VYR08_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV3467300.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(1368..1586)
                     /locus_tag="VYR08_001816"
     CDS             complement(1368..1586)
                     /locus_tag="VYR08_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV3467301.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(1684..2469)
                     /locus_tag="VYR08_001817"
     CDS             complement(1684..2469)
                     /locus_tag="VYR08_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467302.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(2471..3289)
                     /locus_tag="VYR08_001818"
     CDS             complement(2471..3289)
                     /locus_tag="VYR08_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV3467303.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(3291..4283)
                     /locus_tag="VYR08_001819"
     CDS             complement(3291..4283)
                     /locus_tag="VYR08_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467304.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1571 a         1005 c          814 g         1411 t
ORIGIN      
        1 aagattggat agttgcttga ttgctttttc tccagattct ttatctttaa attcagcaat
       61 atagacaagt ctatcttctt ttttcggatt ttctgtgcct ttatttgcag acgagtgtgc
      121 attatctcct tgggattgat tggaatcttc tttgattctt gcttcttcat tgctagtttt
      181 agtgtctatc acaggatttt tactaacaac ttctttttcc tcaataactg ttgttttctt
      241 ttcttcagta tcctttgaag tttctatagc attatgaata tcttcatgtt tctctttatt
      301 ttctgtttcc ttatctaata ctactttttc tttatcagag atgtttaaag catcttcaga
      361 gctagatgtg tctgctaaga ctacctcatt aggggcatag gctgctaaaa taactgcagc
      421 tgtggttaat gacaatactg tacttttttt cattttaatt ccttacatat ttatttaact
      481 tccaatagat aataaacttt aactttgcta gcctttgtta taaaaagttt tactaagtat
      541 tatctaggaa atagagtagt acatttatat ataattgtta tctctccata aaaacagtat
      601 atcatttaaa aaaatttaat tcaagaaaaa ttaacatgca ttaatttatt ttttagcaca
      661 cgttaaaaaa taagattagt actcaatgaa aatcaaagag caaactagga aactagccgc
      721 aggttgctca aaacactgtt ttgaggttgt agatggaact gacgtagtca gctcaaaaca
      781 ctgttttgag gttgtggata gaactgacga agtcagtaac catacgcacg gcaaggcgac
      841 gttgacgtgg tttgaagaga ttttctatga gtataagtcc aaaactttcg ttaaccggga
      901 tacattataa gcacattcat accatcaaaa atagtagcaa taaacttgaa caactaccag
      961 aaacacgaaa acaggcataa ttgatgaaat atcagcgcta ttaaacaata taaataaccg
     1021 ctaagaataa ggcttaacgg ttatcttcta cctatctatt ttttcaactc ctcagtcgtt
     1081 tatctaccta taacctaggc tttacgagtg ccttgctcta ccaactgagc taaatcggcg
     1141 attacactta cagtatggca ctctgagaat agatgtcaag taatattatt caagcttttt
     1201 aatcaaaatc tgacaaggat tttacggatt ccatagctga gaaaaaatct ctaacttttt
     1261 aaagccaaga cctcgataaa agtcatttgt tcgatcataa tctttattag aaccttctgc
     1321 cactgttttg acctgcagat aaccaacttt tttacgagct tcactctcta aagtagcaag
     1381 caattggctc ccaatttttc taccttgata agctttttta cgccgagaca atcaatctct
     1441 gcacaatctt cactcgaata ggataacctt acaaatccag tcaaatcact ctcctgatag
     1501 gcggtccaaa cttgcagtgt cgtggttcct tctatatagg cttgtgtgct ttctgggatt
     1561 ccaaaccatt ctggcaaatc cttcaaaacc tcaacgacaa ctgccttttt tgattttcat
     1621 ccttgacttc ttcaattaca aacatcataa gttcctttct tgattttagt cttactttag
     1681 cttttacgaa cccgcacttt cgtaggaatc taagccctta tcccaaagtt taagggaaat
     1741 aacaaagaaa accagagaaa tcaacatcaa acctcctacg ttaaagaaca catccttttc
     1801 ctgtaagaaa tagctagctg gatagtaggc tgtaaaggcg aaaggcacga taaagctaat
     1861 caaccaacga agaagagaat tgtaaataga aatcggatac ttagcaaagt cattgaacat
     1921 atagaagatg taaatcatgg cgcctgactg cttagtccaa aaggcgatac tggctgttgc
     1981 gattttaaga gaagtataaa tcaaggtcgc aaaaggaata caaactagga aaagcaggaa
     2041 ttttggaaga gtccaaacaa tgctggtcac tgttgttccc aataaaatac caccgactaa
     2101 gagttcaccc aaggcatcaa tctgaaaggt ttcaactagg atgtgaaaga gaggattgat
     2161 gggacgagtc agatacttgt caaactctcc ttttcggact aggcgttgcc ctagtgccca
     2221 gagattgtca aaaaagagat ggtccattcc cttgggaatc aaggaaaatc cataaatgaa
     2281 agctatctct tgaaaggtcc agccttctag gaatggaata tgttgaaaga tgacattgag
     2341 aaacaagaga ttcaagcctt gagccagaaa gactcccaag acaccaacca caaaatccac
     2401 cttatattcc atgatttgtt tgatgtattg tctgataaaa atcagatgca ttcgttgata
     2461 ttttttcata ctaacctcct tgaatggtga taaaggactg gacccgtttc caaattaact
     2521 gagacaatcc caccatcact aagagccaga agaactgcaa aaggagtgcc tgaagaatct
     2581 gactggcatc gtattttcca acaatgatca taactggagt ataaatcaag gacgaaaaag
     2641 gcaaaaagga gagaatatct gaaacaacct ttggaaagaa tgccaaggga atcaaactcc
     2701 ccgacataaa agccactatg gaagtcttaa gtaggttgga accccaaaga tttttaaaca
     2761 caaaggctga aaatccaaag caaatattaa agaaaaagtt aatcagatag gcgagcgtta
     2821 agctaaaaag ataaaggaca gttaatccta gcacctctac aataccttga cccgatatga
     2881 ttttcatcaa gacaatgaca cttaaaaatg gaaggccaac gctgataaaa atcaaccact
     2941 tggaaccaag ctcggtgaaa agataggagg ccgcaaaatg cactggtcgc aacaaacgca
     3001 tgataatgga gccatccttg acctcctccc caatcataaa ggacgaatcg gatctagtca
     3061 gaagattggt cacaaaactc atgatgatgt agagggtgat atccgccata ctgaagccct
     3121 gaatcaaaga ctcttgcgaa gaatcaaaga cagccttcca gagataaaag gccacaaaag
     3181 cccccatgac atcgccaatc cgatagagaa taaagttgac tcggtaagta atcaactcct
     3241 gaacccctgc attgataaag ggtttataac gtctccacaa tttgatcatc ctagagctcc
     3301 tttcggtaga agcgacggat aatatcctca atatccgtat ccaccatctt caaatcgcgg
     3361 atttcaaaat cagatagggt ttgcttgata atgtcagctg actggtagcg agaactatca
     3421 aattcaatgt tgaggctgtt tccttgtcta tcaatggtca tatcagacag accttcatag
     3481 tgagagacga gatgactttg acctggtagc agttcaaaag agagagtctt catcttacca
     3541 aaggtctcct tgagttggct caccgttcca tcaaaaatct tttgcccctt gtcaatcatg
     3601 aaaatccgat cacaaagttg ctcaatatca ctcaaatcgt gagtggtcaa aagaatggta
     3661 gtttcttcct cttgattgat ctgagtaatt gcccgacgaa tattatcctt aaccgaaacg
     3721 tccaaaccaa tggtcggctc atctaaaaaa agaaccttgg gattgtggag caaggaggcc
     3781 gcaatatccg ctcgcatccg ttgtcccagt gaaagagtcc gcacgggatc cttgataaag
     3841 tccttcaaat ccaagacttc attcaaaaag tccatacgct tatgaaagag cgagtttggc
     3901 acatcataaa tctcttttaa gacagtgtag gtctcttgca gagccaaatc ccaccatagc
     3961 tgggtgcgtt gtccaaagac tacgccaata tctttgacat aatcttgccg attgtcctgg
     4021 ggaatcttgc cgttaatccg acaaaaacca gatgttggtt tcaaaattcc tgtcagcatt
     4081 ttaatggttg tcgacttccc agcaccattt gccccgataa atcctaaaat ctgccctttt
     4141 ggaacctcaa aggtcaaatc cttgaccgct tcaaaggtct gcttttcagg atgaataaag
     4201 gagcgcaaag cccccttcaa gcccggttcc ttaacaatct tcacaaaatg tttctgaaga
     4261 tgttccactt ctatcattgc catatctatc tccctatcgt aaggaatagc aacattgtgt
     4321 ttttaactac aaatattcta aatccttact ttctacacct tctacatttt attactacag
     4381 aaggctttat accaacctat tatagtccaa taaaaaacga aatgcaagcg tttgcctata
     4441 gattgtgaaa ttaggatttc tctcatgaaa taatactttt aatcaaaaat tctgtttaat
     4501 agtttaaatt accaacctac tcatcttctc accagagggc ggtattaact ttgatcataa
     4561 aatcggatac tccctctatc cagaaaatat gctcagaatg attgtctcta aacaatacct
     4621 gctcttttac ctcattcatg aaaaagaagt ccataccctg agaattatca actcacgcac
     4681 cgactaccta aaccaactcg accacctctt tcgtacatgt aggaaactat tttcctcaca
     4741 aataatctca ttataaaatc caataaattt ttttagctca cttgaatatt ttctttgtat
     4801 c
//
LOCUS       DAVMHG010000134         4708 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMHG010000134 DAVMHG010000000
VERSION     DAVMHG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.136"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..222
                     /locus_tag="VYR08_001820"
     CDS             7..222
                     /locus_tag="VYR08_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467305.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(331..1071)
                     /locus_tag="VYR08_001821"
     CDS             complement(331..1071)
                     /locus_tag="VYR08_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV3467306.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(1071..2636)
                     /locus_tag="VYR08_001822"
     CDS             complement(1071..2636)
                     /locus_tag="VYR08_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV3467307.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            2774..4663
                     /locus_tag="VYR08_001823"
     CDS             2774..4663
                     /locus_tag="VYR08_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV3467308.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT         1294 a          863 c         1101 g         1450 t
ORIGIN      
        1 aaaaacatgg ctaagtcaaa ctttgaaaaa gtagaatcag ttgttggctg ggttcgtgat
       61 aagaaaatca caggctaccg tatctctaaa gaaacgaatg cgcgtgaaat gtctatcatt
      121 gctctggcgc agggtcgtgc aaaagtaaaa aatatttcat ttgaaacagc cctaggccta
      181 attgatttct atgaaaaaaa ttatgaaaaa tttgaagatt aatctttgga taacggcgga
      241 ttcttgacct tcaagtagta gagatagaga atctgccttt tcattttgag gacagcaaaa
      301 agactgcacg gttgatgcag ccttttcttt ttatttgaga tagcgttgaa ggaactcttt
      361 tgttcggtct tctttaggat tggtgaagag gtcttctggt ttaccttctt cagcgatcac
      421 gcccttatcc ataaagataa cacggtgaga gacatcacgg gcaaattcca tttcatgggt
      481 tacgacaatc atggtcaagc cttcctgagc caggtcctgc atgattttga ggacttctcc
      541 aaccatttct ggatcgagag ctgatgttgg ttcatcaaag agaatggcgt ccggattcat
      601 ggagagggca cgagcgatgg ccacacgttg tttttgacca cctgagagtt gttttggttt
      661 ggcttgccag tagcgttctc ccatgccgac cttttccagg ttttctttgg caatcttttc
      721 agcttctgtg cgttcgcgtt ttaggacagt tgtctgagcg acgattgtgt tttcaagaac
      781 attgagattt tcaaagaggt taaaggattg gaaaaccatc cccaactttt cacggtattg
      841 cgtgaggtca tagccttttt cgaggacgtt ttgtccatga taaaggattt gtccatcagt
      901 tggtgtttca agtaggttaa tggagcgtag gaaggtcgat tttccgcttc cagagcttcc
      961 gatgatagag atgacctctc ccttgtggac agtgagtgaa atgtctttta gcacttcgtt
     1021 ttgtccatag gattttttga ggtgtttaat ttcaaggatt gcttgtgtca ttatttcaaa
     1081 tcctccgttt gcatttggtt agcacctgta gtgtaggtat ccatgtccat tctgcgctcg
     1141 ataaagcgta ggatacgtgt tacggtgaag gtgaggacaa agtaaatcac ggcgatgatt
     1201 gtaaatgtct ggaagtattg ataggtttgt gttgccacgg tatttcctga gaaataaagt
     1261 tcgacaacag agataacgtt caatacagat gtatctttga tattgatgac aaattcatta
     1321 ccagttgcag gtaggatgtt acggactacc tgaggtagga caatcttacg catggtctgg
     1381 ttatgggtca taccaagagc agtcgcagct tcaaattgtc ccttgtcaac tgctaggata
     1441 ccaccacgga cgatttcagt catgtaggca ccggtattga ttgaaacgat gaagatagca
     1501 gctagtgtac ggtcaaggtt gatcccgaaa gcttgggcag ttccatagta gataaccatc
     1561 gattgaacaa tcattggcgt accacggaaa atttcaatgt agacattgag aacccagccg
     1621 actagttttt gaaggccata aatggctttg ttttcagaga gaggagcagt acggaagaca
     1681 ccaatggcaa gtccaataat gagacctatg atggttccga cgatagagat taaaagagtg
     1741 ataccagcac cacgcaagag ttgttgccag ttttcagaaa gaattttagc gacttggcta
     1801 aagaaactac tgctagtctc ttcagttgtt gtagcttcgg caggttgttc cttgatcata
     1861 cgatccatca aggcaacttg gtcatctttt gaaatggttt caatgctggc attgatttgg
     1921 ctaatacgat tgtcattttt acgaagcccg atagcgatag ctgtatcttc ttccccagtt
     1981 ttgaaaccag gttctacttg aatcatcttg aacttagagt tcgcagcttc agcagtcagt
     2041 gcttctggac gttcagaaac ataagcatca atgacaccag cctcaagagc ttggcgcatt
     2101 tgagcgaagt ctcccatggc tgtttctttt ttagcacctg ggatttgtgc aatcaagtcg
     2161 tagaggtaga ccccttgttg agaagtgatt tttgcaccgt taaagtcatc caaagattta
     2221 gcacttgcgt aggcagaatc ttttttgaca agcaaaactg gttcgctagt gtagtaactg
     2281 ctcgaaaagg caatttcttg tttgcgttct gcagttggac tcatacctgc gataatcatg
     2341 tcaatcttac cagaagtaag ggcagggact agaccttccc acttggtttt aacaaccaaa
     2401 ggttctttac ctaagtcctt agcgattttc ttggcgattt gaacatcgta tccgttggca
     2461 tactgattgg tcccatcgat tttgacagct ccgttgctat catcatcctg ggtccagtta
     2521 aagggagcat atgctgcttc catcccgatg cgtaaatatt catcggcttg agcaacattg
     2581 acaagtccta gcatcagcaa gagacttgtg aaaatagata agtatatttt tctcatgatt
     2641 tctcctattc tgatctatta aaaaataact gtctcctatt ttatcgaaaa atgcgtaatt
     2701 tttcaacata agtaagcctt tacttacgaa aaaatgctat aatgataaga aagataaaaa
     2761 gggggcttag ttgatgaaaa aaactttttt cttactggtg ttaggcttgt tttgccttct
     2821 tccactctct gtttttgcca ttgatttcaa gataaactct tatcaagggg atttgtatat
     2881 tcatgcagat aatacggcag agtttagaca gaagatagtt taccagtttg aggaggactt
     2941 taagggacaa atcgtgggac ttggacgtgc tggtaagatg cctagcgggt ttgacattga
     3001 ccctcatcca aaggttcagg ccgcgaaaaa cggtgcagaa ctagcagatg tgactagcga
     3061 agtaacagaa gaagcggatg gttatactgt gagagtctat aatccaggtc aggagggcga
     3121 catagttgaa gttgacctcg tctggaactt aaaaaattta cttttccttt atgatgatat
     3181 cgctgaatta aattggcaac ctctgacaga tagttcagag tctattgaaa agtttgaatt
     3241 tcatgtaagg ggagacaagg gggctgaaaa actctttttc catacaggga aactttttag
     3301 agagggaacg attgaaaaga gtaaccttga ttatactatc cgtttagaca atcttccggc
     3361 taagcgtgga gttgagttgc atgcctactg gcctcggacc gattttgcta gcgctaggga
     3421 tcagggattg aaagggaatc gtttagaaga gtttaataag atagaagact cgattgttag
     3481 agaaaaagat cagagtaaac aacttgttac ttgggtcctc ccttcgatcc tttctatctc
     3541 cttgttattg agtgtctgct tctattttat ttatagaaga aagaccactc cttcagtcaa
     3601 atatgccaaa aatcatcgtc tctatgaacc accaatggaa ttagagccta tggttttatc
     3661 agaggctgtc tactcgacct ccttggagga agtgagtccc ttagtcaagg gggctggcaa
     3721 attcactttt gaccaactta ttcaagctac cttgctagat gtgatggacc gtgggaatgt
     3781 ttctattatt tcagaaggag atgcagttgg cttgagattg gtgaaagaag atggtttgtc
     3841 aagatttgag agagactgtc taaatctagc cttttcaggc aaaaaagaag ccactctttc
     3901 caatttgttt gcggattaca aggtatctga cagcctttat cgcggagcta aagctgctga
     3961 tgagaaacgg attcaagcaa gagggattca gctcaaatct tctttcgaag cagtattaaa
     4021 agagatgcaa gaaggagtga gaaagcgagt tgtcttctgg ggactcccag attactatcg
     4081 tcctttaact ggtttggaaa aggctttgca agtgggtatg ggtgtcttga ctatcttgcc
     4141 cctatttatc ggatttggtt tgttcttgta cagtttagac gttcatggct atctttacct
     4201 ccctttgcca atacttggtt ttctagggtt agttttgtct gttttctatt attggaagct
     4261 tcgactagat aatcgtgatg gtgttctaaa tgaagcggga gctgaggtct actatctctg
     4321 gaccagtttt gaaaatatgt tacgtgagat tgcacgactg gataaggctg aattggaaag
     4381 tattgttgtt tggaatcgtc tcttggtcta tgcaacctta tttggctatg cggacaaggt
     4441 tagtcatttg atgaaggttc atcagattca agttgaaaat ccagatatca atctctatgt
     4501 agcttatggc tggcacagta tgttttatca ttcaagcgcg caaatgagcc attatgctag
     4561 tgtcgcaaat acagcaagta cttactctgt atcttctgga agtggaagtt ctggtggtgg
     4621 cttctctgga ggcggaggtg gcggcagtat cggagccttt taaagagagc taccatagac
     4681 tgaaaaagta tgatataatg gaagatag
//
LOCUS       DAVMHG010000135         4434 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMHG010000135 DAVMHG010000000
VERSION     DAVMHG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.137"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(132..1334)
                     /gene="rpsA"
                     /locus_tag="VYR08_001824"
     CDS             complement(132..1334)
                     /gene="rpsA"
                     /locus_tag="VYR08_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV3467309.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1496..1567)
                     /locus_tag="VYR08_001825"
     tRNA            complement(1496..1567)
                     /locus_tag="VYR08_001825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1533..1535),aa:Gln,seq:ttg)
     gene            complement(1577..1657)
                     /locus_tag="VYR08_001826"
     tRNA            complement(1577..1657)
                     /locus_tag="VYR08_001826"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1621..1623),aa:Tyr,seq:gta)
     gene            complement(1707..1937)
                     /locus_tag="VYR08_001827"
     CDS             complement(1707..1937)
                     /locus_tag="VYR08_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV3467310.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2105..2749)
                     /gene="pcp"
                     /locus_tag="VYR08_001828"
     CDS             complement(2105..2749)
                     /gene="pcp"
                     /locus_tag="VYR08_001828"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV3467311.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIETIVDYKDKDLQLVGGETH"
     gene            complement(2764..3687)
                     /locus_tag="VYR08_001829"
     CDS             complement(2764..3687)
                     /locus_tag="VYR08_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV3467312.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3684..4373)
                     /locus_tag="VYR08_001830"
     CDS             complement(3684..4373)
                     /locus_tag="VYR08_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV3467313.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT         1406 a          995 c          680 g         1353 t
ORIGIN      
        1 tgaacatcaa aaaagaaagg acgaaatttg tcctttctca agcttagctg acttcaaccc
       61 actacagttg acaaagagcc agaaaaatcc cttgtttagt aaacaaagga ttttgtgaat
      121 tttcaatttg attaaagttc gatatcacca aacaaatcag ccattgaaaa tcctgtttgt
      181 gtttctggaa gttcgaaatc acgcttttct tgacgtcttg gacgacgtgg acgagcagca
      241 cgtttttctt ctttttgtcc ttcttcttgg gctggacgtt cttcaagagc tttaatagaa
      301 agtgacacgc gttctgcatc tgcgttaact tcaagaactt taacttgaac ttcttgacca
      361 actttaagag cttcttttgg attttcaatc cgtttgtgtg aaatttgtga tacgtgaaca
      421 agtccatcga tacctggcaa tacttcaaca aatgcaccga agtcagtcaa acgtttaact
      481 gttccttcta ctacatcacc tttagccaat ttttgctcaa cgccatccca tggtcctggt
      541 actgttgctt taagtgaaag tgatacacgt ccttcttctt cgttaagatc aaggattttc
      601 acttcaattt cttcaccaac agttacaact gattttggtg atacgttacg ttcatgtgac
      661 aattcagtca agtgaaccaa tccgtcaaca ccaccaaggt cgatgaaagc gccgaagctt
      721 gtgatacgag caactttacc agttacaaca tcaccaacag ccaatttacc gaatacttca
      781 gcgcgagctg ctgcagtagc tgcttcaaca acttcacgac gtgaaaggat gaagcggttt
      841 tctttagcgt taacttcttt gattttagta tcaaattctt gacctacaaa acgctcagcg
      901 ttacgtacga aacgagtatc caacattgaa gctgggataa atccacgaac accttcaaat
      961 tctactgaaa gtccaccttt aacggcacgc gttcctttaa cagtaacaac ttcttcttcg
     1021 cgaccaacaa gtttgtccca tgctttgcga gcttcaaggc gttttttaga tacaaggtat
     1081 gtaactgtat cagtatcttt accaactact tgacgaagta caagaacatc caatacttct
     1141 cctactttaa caaagtcatt gatatctgca tcacgatcgt ttgtcaattc gcgaagagtc
     1201 aagacacctt caacaccagt tccagagatt gcaacgttag cttgagtcgc atcaactgtc
     1261 aatacttcag cactaacaac atcaccagtc tcaacttgac taacgctatt tagcaaatct
     1321 tcaaattcgt tcatctaaaa aatcctccaa caatcaagtt tttcttaaac ttgacatact
     1381 tataattttt ttcctaagca cccgcaagga catgttcata tcattcacgt ctttcaatta
     1441 taaaaataaa ataccctaag atattttgct ttctatcttg attattacct aagaactggg
     1501 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     1561 taccccatta atgaaatgga gagagaggga ttcgaacccc cgaacccgaa ggagcggatt
     1621 tacagtccgc cgcgtttagc ctcttcgcta tctctcctac aatcaacatg gactattata
     1681 tcatgaaaat ttcaaaaaaa caagacttat tttgctaaat tataattttc aatcaaaatt
     1741 tccacagctt tttccaattt tttataaaaa ctatcgacat tcccattctt tataatggca
     1801 aattgtccgt ctaattcggc aatttctccg ataacttttt taccgatagt caatgtataa
     1861 ccttcaaaac tgtctttacc aacattaact ttggcatccg ctacttgaat ttcgattttt
     1921 ttatctttct tgctcatttc atacctctct tcactttttc tattttacaa gaaaatccta
     1981 aaactagcaa gaaaaaactc tatatcaggc ttctctgata tagagtttta tgtaaacaac
     2041 ctcaatcgat ttagcatagt taatagttag taaacaagca taattgacta tcctttttag
     2101 gttatcagtg agtctcaccg cctactaact gcaaatcctt atctttgtag tctacaatag
     2161 tttcaatagc tacctctatg cctcttacaa tatcacatag actcattcct gcagtattcg
     2221 gcttatttac tacttgttcc atcatatatg gaatatgcat gaaacctgct ctcatattag
     2281 ggaatttttt atccactaaa taaagagctt ggtacatcaa atgattgcaa acaaaggttc
     2341 ctgcactatt ggatacaact gccggaagtc cctgtttttt gatagcttgt accatcgctt
     2401 tgataggtaa actactaaaa taggccgatg ctccatcaat acgaatcggt gtatcaattg
     2461 gttgattgcc ttcgttatca ggtatgcgag catcatcttg attaatagcc actcgttcag
     2521 gtgttaagcc ggtcctgccg cctgcttgtc caatacaaag tacagcatct ggttgatatc
     2581 gtaatatttc tgcctctaaa acttctgacg acttataaaa aaccgttgga atttctaccc
     2641 agcgaacttc agccccatta atctcagatg gtaataattt tacagcctcc aaagctggat
     2701 taatcttttc acctccaaaa ggattaaaac ctgtaaccaa tattttcatt ttattttcct
     2761 ttactaaaat gcgagaaagt acattaagaa tatgtgaata acaatcatta ctagagcaac
     2821 acctgcttga gcctttataa cgccattctg atctttcata tccatcaatg ctgctggtag
     2881 agcgttaaaa ttagcagcca ttggggtcaa taaggtccca caataacctg ctgtcatggc
     2941 aagagcacca gccacaattg gattagctcc cagagcaaat acaaagggaa ctccaacacc
     3001 tgctgtaata acggtgaatg ctgcaaaagc atttcccata atcattgtga atagaaccat
     3061 tccaagaaca taagccaaaa ctcctataaa gcgactatct gaaggaacaa taccgctaat
     3121 cagatgagag ataacatcac caacacctgc tacagtaaaa atagccccca aagcccctaa
     3181 taattgagga acaatcccac ttgttgaaac ttgctgagtc attcgattat tttctgataa
     3241 cagactctta gggtgactat tggtaatcac aagaacagaa attgtagcaa acaaggcggc
     3301 aaggctaatc gaaatcttgc taaattctgg aatcatttgc gctaagacca acgcaagtat
     3361 tgccatcagc ataactggaa taaaaatttt atttttcaac ctgttagatt caatattggc
     3421 tttcatttca tctaaggatg gcaaggttcc gatacggact tgcttaaaca atgttaacag
     3481 cgataatagg attacaataa taccaatact catatttggc atataggaac cacctataaa
     3541 cgtaatagac aatagagtcc aaaatgcaga tgtcccaagt cgaactgggt ttgttttatc
     3601 tttataacta caataggctg tatggagaaa ttgacaacca atcacaatat aggtcaactc
     3661 taatagttgc tttgccaact ctgtcatttt tgttctcctc ccctagtctt ttttgatatc
     3721 aattttttat caaataaata attgtaaatc cccactacaa taagtgttat aacagcaaca
     3781 ataatagatg tagaagcaat ccctgcataa ttgctttcat agcctaactg atctaatgtt
     3841 ccccctatca agaggactcc cccagcacct acaaacgtat tttgagcaaa gaaatttcca
     3901 aaattttcat tcgctgccgc acgcgctttt attgtctcat cttcaacctc tgttaacttt
     3961 ctacctaatt gagactctgc agctgcttct cccataggtt gaaccaaagg tctgacaaac
     4021 tgagggtgtc ctcctagacg aattgaaaag aaaccagcta actctcgaat aaagaaataa
     4081 actgtataga agtttccaac tgtcagacct ttaatctttc gaatcaaatc gattgatcgt
     4141 tgcttgagtc caaaggtttc tgacagcccc acaagaggca aggtaaccat aaaaatcgtg
     4201 agcactcgct gattgctaaa ttcttttccc aaaatctcca aaaattcaac gagagaaaca
     4261 cctgaaacta aagctgtaac caaaccagct aagactactg ttgcaattgt atcaaatttt
     4321 aaaataaaac ccacaacaat gattgctatt cctattaatc taatccactc catatcaaac
     4381 tcctttatat tcaaaatgac agtattttaa aaattttatc aagatcaata ccat
//
LOCUS       DAVMHG010000136         4423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMHG010000136 DAVMHG010000000
VERSION     DAVMHG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.138"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            361..1407
                     /gene="pheS"
                     /locus_tag="VYR08_001831"
     CDS             361..1407
                     /gene="pheS"
                     /locus_tag="VYR08_001831"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV3467314.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKGMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1407..1916
                     /locus_tag="VYR08_001832"
     CDS             1407..1916
                     /locus_tag="VYR08_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467315.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1993..4395
                     /locus_tag="VYR08_001833"
     CDS             1993..4395
                     /locus_tag="VYR08_001833"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV3467316.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
BASE COUNT         1270 a          891 c         1053 g         1209 t
ORIGIN      
        1 attaatttta ctttcccaat cgatttgttt tcatcttatt tcaatccgct ataaagttag
       61 ataagaataa gctaacaagc agaagtaaga taggtcttgc aagaatggta aaaatctgat
      121 aaactaagta gtaattgaat aggaatctga aagactagta gcccaaggga agtatatcag
      181 ggaggagagc cgtgactgca agctctctat atgaaagctg ggtgaattca cttgcgcatg
      241 gatttagaaa tgaaggtctg acttgtcaga tgaaaacgga tggtaccgcg tgtcaacgct
      301 ccgagtggag tttttggcat gtggttttct ttttatctac gagagactga tggaggaaat
      361 atgtcaacta ttgaagaaca attaaaagcg cttcgcgaag aaacgctggc tagcttgaag
      421 cagattactg ctggaaatga aaaagggatg caagatttgc gtgtctctgt ccttggtaaa
      481 aagggttcgc tcactgaaat cctcaaaggg atgaaagatg tttctgctga gatgcgtcca
      541 atcatcggga aacacgtcaa tgaagctcgt gatgtcttga ctgctgcttt tgaagaaaca
      601 gctaagctct tggaagaaaa gaaagtcgcg gctcaactgg ctagcgagag tatcgatgtg
      661 acgcttccag gtcgtccagt tgcgactggt caccgtcacg ttttgacaca aaccagtgaa
      721 gaaatcgaag atatcttcat cggtatgggt tatcaagtcg tggatggttt tgaagtggag
      781 caagactact ataactttga acgtatgaac cttccaaaag accacccagc tcgtgatatg
      841 caggatactt tctatatcac tgaagaaatc ttgctccgta cccacacgtc tccagttcag
      901 gcgcgtgcta tggatgccca tgatttttct aaaggtcctt tgaagatgat ctcgccaggg
      961 cgtgtcttcc gtcgcgatac ggacgatgcg acccacagtc accaattcca ccaaatcgaa
     1021 ggcttggtag ttgggaaaaa tatctctatg gctgatcttc aaggaacgct tcagttgatt
     1081 gtccaaaaaa tgtttggtga agagcgtcaa attcgtttgc gtccatctta cttcccattc
     1141 acagagccat ctgttgaggt ggatgtttct tgcttcaagt gtggtggaga aggctgtaac
     1201 gtatgtaaga aaacaggttg gatcgaaatt atgggagccg gtatggttca cccacgtgtc
     1261 cttgaaatga gtggtatcga tgcgactgta tactctggtt ttgcctttgg tcttggacaa
     1321 gagcgtgtag ctatgctccg ttatggaatc aacgatatcc gtggattcta ccaaggagat
     1381 gtccgcttct cagaacagtt taaataatga ttagaaaagt agaaatggca gatgttgagg
     1441 tgttggctaa aattgccaaa caagcctttc gtgaaacctt tgcgtatgat aatacggaag
     1501 agcagttaca ggaatacttt gaagaggctt atagtctgaa aactttgtca actgagttgg
     1561 gaaatcctga ctctgaaacc tatttcatta tgcatgagga ggagatagct ggttttctca
     1621 aagtcaactg gggaagtgct caaactgaga gagaattaga ggacgctttt gaaattcaac
     1681 gcctctatgt gctacaaaaa ttccaaggat ttggactagg taagcaactg tttgaattcg
     1741 cacttgaact tgctacaaaa aatagttttt cttgggcttg gctaggtgtt tgggagcata
     1801 atacaaaagc tcaagcgttt tataatcgat atggttttga aaaatttagc caacatcatt
     1861 ttatggttgg tcaaaaagta gatacggatt ggttactgag aaagaaatta aggtaagaag
     1921 aggattcttc tattgaaatg ataggaaagg aaaagaacca gtgtggcaat ggtcattctg
     1981 gagaactaaa ttatgcttat atcttataaa tggttaaaag aattggtgga cattgatgtg
     2041 ccatcacaag agttggctga aaaaatgtca actacaggaa tcgaggtaga gggtgtcgaa
     2101 tcaccagctg ctggtctctc aaaaattgtc gtcggtgagg tcttgtcttg cgaagatgtg
     2161 ccagagactc acctccatgt ttgtcaggtt aacgttggcg aagaagagcg tcagatcgtt
     2221 tgtggtgccc caaatgtgcg tgctgggatc aaggtcatgg tggctcttcc aggagctcgt
     2281 atcgctgata actacaaaat caaaaaagga aaaatccgtg gtttggagtc acttggaatg
     2341 atctgttcac ttggtgaatt gggaatttct gactcagttg tgcctaagga attcgcagat
     2401 ggcatccaaa tcttgcctga agatgccgtg ccaggtgagg aagtcttttc ttacctagac
     2461 ttggatgatg aaatcatcga actttccatc acaccaaacc gtgcagatgc cctttctatg
     2521 tgtggagtgg ctcacgaagt ggcagccatc tatgacaagg cagtcaactt taaagaattt
     2581 actctaacag aaactaatga agctgcggca gatgcccttt ctgtcagcat tgagacagac
     2641 aaggcgcctt actatgcagc tcgtatcttg gacaatgtga ccatcgcacc aagtccacaa
     2701 tggttgcaaa atcttctcat gaacgaagga atccgtccca tcaataacgt agtggacgtg
     2761 accaactaca tcctgctcta ttttggtcaa ccaatgcatg cctttgactt ggataacttt
     2821 gaagggactg acatccgtgt gcgtgaagcg cgtgctggtg aaaaattggt gaccttggac
     2881 ggtgaagaac gtgacttgga cgtgaatgac ctagtcatca ctgtcgcaga caagccagta
     2941 gcccttgcag gtgtcatggg tggtcaagca acagaaatct ctgaaaaatc tagtcgtgtt
     3001 gtccttgaag ctgctgtttt caatggcaaa tctatccgta agacaagtgg tcgcctgaac
     3061 cttcgttctg agtcatcttc tcgctttgaa aaaggaatta atgtggcaac agttaatgaa
     3121 gcccttgatg cggcagctag cctgattgcg gaacttgcag gtgcgacggt gcgtaagggc
     3181 atcgtttcag cgggtgagct tgatacttca gatgtagaag tttcttcaac ccttgctgat
     3241 gttaatcgtg tcctcggaac tgagctgtct tatgctgatg tagaagacgt cttccgtcgt
     3301 cttggctttg gtctttctgg aaatgcagac agctttacag tcagagtccc acgtcgtcgt
     3361 tgggatatca caatcgaagc tgacctcttt gaagaaattg ctcgtatcta tggttatgac
     3421 cgcttgccaa ctagtctacc aaaagacgat ggtacagcag gtgaattgac agccacacaa
     3481 aaactccgcc gtcaagttcg tactattgct gaaggagcag gtttgacaga aatcatcacc
     3541 tatactctaa caactcctga aaaagcagtt gagtttacgg ctcaaccaag taaccttacg
     3601 gaactcatgt ggccaatgac tgtggatcgt tcagtcctcc gtcaaaatat gatttcaggt
     3661 atccttgata ccgttgccta caacgtggct cgtaagaata aaaacttggc cctttacgag
     3721 attggaaaag tctttgaaca aacaggtaat ccaaaagaag aacttccaaa tgaaatcaac
     3781 agttttgcct ttgccttgac aggcttggtt gctgaaaaag atttccaaac agcagcagtt
     3841 ccagttgatt tcttctatgc taagggaatc cttgaagccc tatttactcg tttgggactc
     3901 caagtaacct atacagcaac atctgaaatc gctagccttc atccaggtcg tacagccgtg
     3961 atttcattcg gtgaccaagt tcttggtttc cttggccaag tgcatccagt cactgccaag
     4021 gcttacgata ttccagaaac gtatgtggct gagcttaacc tttcagctat cgaagctgcg
     4081 cttcagccag cgactccatt tgtagaaatc accaaattcc cggcagtcag ccgtgacgtt
     4141 gcccttctcc tcaaggcaga agtgactcat caagaagttg tagatgctat ccaagctgcc
     4201 ggcgtgaaac gtttgacaga tatcaaactc tttgacgtct tctcaggtga gaaattggga
     4261 cttggtatga agtcaatggc ttatagcttg accttccaaa atccagaaga tagcttaacg
     4321 gacgaagaag tcgcacgcta tatggaaaaa atccaagcat cgctcgaaga aaaagtcaat
     4381 gcagaagtgc gttaaacgat agtatgatac aaattccccc tct
//
LOCUS       DAVMHG010000137         4400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMHG010000137 DAVMHG010000000
VERSION     DAVMHG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.139"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            356..973
                     /locus_tag="VYR08_001834"
     CDS             356..973
                     /locus_tag="VYR08_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV3467317.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(1293..2180)
                     /locus_tag="VYR08_001835"
     CDS             complement(1293..2180)
                     /locus_tag="VYR08_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV3467318.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(2329..4212)
                     /locus_tag="VYR08_001836"
     CDS             complement(2329..4212)
                     /locus_tag="VYR08_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3467319.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         1270 a          975 c          867 g         1288 t
ORIGIN      
        1 acaattcaaa ggattcatac tagccataaa ttacgtccat cagagagaga ctcttactac
       61 ttttagattt tagtctttct agcttcagaa tacatctaaa ctttagggaa aatgactatt
      121 cgaaagcgcg aatgcctcaa aattatctca gataagctat tcgaaactta gaatgctttt
      181 aaatttatgg aattgcgatt attcgaaacc tagaatgcat ataaccttta gttgacagac
      241 ctattctaag tctcgaaggg ctatttactt tctattcctt atcaaaaaag actcattccc
      301 cctttctcct ccaaaatatg gtatagtaga aatatactat ctatgaggag tttacatgtc
      361 acaggataaa caaatgaaag ctgtttctcc ccttttgcag cgagttatca atatctcatc
      421 gattgtcggt ggggttggga gtttgatttt ctgtatttgg gcttatcagg ctgggatttt
      481 acaatccaag gaaaccctct ctgcctttat ccagcaggca ggcatctggg gtccacctct
      541 ctttatcttt ttacagattt tacagactgt cgtccctatc attccagggg ccttgacctc
      601 ggtggctggg gtctttatct acgggcacat catcgggact atctacaact atatcggcat
      661 cgtgattggc tgtgccatta tcttttatct agtgcgccta tacggagctg cctttgtcca
      721 gtctgtcgtc agcaagcgca cctacgacaa gtacatcggc tggctagata agggcaatcg
      781 ttttgaccgc ttctttattt ttatgatgat ttggcccatt agcccagctg actttctctg
      841 tatgctggct gccctgacca agatgagttt caagcgctac atgaccatca tcattctgac
      901 caaacccttt accctcgtgg tttataccta cggtctgacc tatattattg actttttctg
      961 gcaaatgctt tgacacgtaa aaaatccgtt tggtttccca agtggatttt taaagcgtag
     1021 attaactata gcttgatact aaatatactt tggtatggaa atcatgcata tttttcgata
     1081 gtgaagcgag gacttaccta gcctttccac cgtgatagaa acacctgaaa tctaatggtt
     1141 tcaggtgttc ggaaactttg agcctagtgt ctcaaagttt aggtatggaa tttcgaagaa
     1201 agtcgctacc gtccgtaatc acttaaggaa aggctcaaaa atattgtttt caaccacaaa
     1261 atccgtttgg tttcccaagc ggattttgtg ctttattttg aaacttcttt tgcaagaaca
     1321 aagttcccaa gtgtggcaga accatttcct gcgactgctg gcgtcacgat atagtcacgc
     1381 acatctggta ctggtaggta accattaaga agagatgtaa atttctcacg gacacggtcc
     1441 agcatatgtt gttgagccat gacccctcca ccaaagacaa tcacgtctgg gcggaaagtc
     1501 actgtcgcat taaccgcagc ttgagcgata tagtaggctt gaacatccca aacagggttg
     1561 ttgagttcaa tagtttcccc acgtacacct gtacgagctt ccaaacttgg accagctgca
     1621 tagccttcca gacatccctt atggaaagga caaacaccct taaactcttt ttcaatatcc
     1681 attgggtgtc tagcaacata ataatgaccc atttcagggt gacccacacc accgataaac
     1741 tcaccacgtt ggatgacgcc tgcaccgata cctgtaccga ttgtgtagta aaccaagttt
     1801 tcgatacgac caccagcatt gttacgggca accacttcac cgtaagcaga gctgtttacg
     1861 tctgttgtga agtacattgg cacgtttagg gcgcgacgaa gggcaccaag caagtccaca
     1921 tttgcccagt ttggttttgg agtcgtcgtg ataaagccat aagtttttga gtttttgtca
     1981 atatcaatcg gcccaaatga accaactgca agaccagcaa ggttatcgaa ttttgagaag
     2041 aactcaatgg ttttatcgat tgtttcgatt ggagttgttg ttggaaattg tgttttttct
     2101 acaacgttaa agttttcatc accgacagca cagacaaact ttgtaccgcc agcttccaag
     2161 cttccatata attttgtcat gataaacctc ttgtttttat tttctttatt atagcatact
     2221 tcgaaagtct aaatgtctct attttttaga ttttcctctg taaatcttac tatctaataa
     2281 aaacgaacaa acatgtcatt tgttcgtttt cacattagag aggattgatt agattttcac
     2341 ttcgatcaca gcatccccct tcgcaactga acctgttgcg actggagcta ctgaagcgta
     2401 gtcagctgta tttgtaacga taaccattgt tgtatcatca agtccagctg tagcgatttt
     2461 atttgagtca aatgttccaa gaacatcgcc aactttcacc ttatcacctt gagcaacttt
     2521 tgcttcaaaa ccgtcaccgt tcatagatac agtgtcaata ccaacatgaa tcaaaacttc
     2581 agcaccattt cttgttttca aaccaaaagc gtgccctgtt ggaaaggcaa ttgaaacttc
     2641 agcatcagct ggtgcataga ccacgccttg gcttggtttc acagcgatac cttgtcccat
     2701 agctccactt gagaagactg ggtcattgac atcagcaaga gcgacaacat caccgacgat
     2761 aggagttaca agtgtttcat tttgaagagc tgctggcgca acttcttctt tttcttcagc
     2821 cacttcagct tgttttgcag ctgcagttgc gtctacttca tcttcgtaac caaacatgta
     2881 agtaagagca aaaccaaggg caaatgatac agctaccata agaaggtatt gtggaagttg
     2941 tccgttacca acataaagca ttgtaccagg gatgatggtg ataccattac cagtaccagc
     3001 aagtccaagg atagaagcca atccaccacc gattgcacca gcaatcaatg aaaggaagaa
     3061 tggtttacgg aagcgcaagt tcaccccgaa gatagcaggc tctgtaatac ctaggaaggc
     3121 agaaagagca gccgggaaag caagtgtttt cagttttgga ttttttgttt taacaccaac
     3181 cgcaacagta gcagcacctt gagctgtcat agcagctgtg atgatagcgt tgaatgggtt
     3241 agcatggtca gcagcaagta attgcacttc aagcaagttg aagatgtggt gcacacctga
     3301 cacgacgatc aattggtgaa ccccaccaat caagaaacca ccaagaccaa atggcatgct
     3361 aagaatcgct tttgtagcaa taaggatgta gttttcaaca acgtggaaaa ctggtccaat
     3421 gacaaagagt ccaaggatag acatgaccaa aagtgtcacg aatggtgtta ccaagaggtc
     3481 aatgacatct ggaacaacct tgcggacagc tttttcaaat ttagctccga caaccccgat
     3541 gatgaaggct ggaagaacgg aaccttgcaa accaacaaca gggatgaaac caaagaagtt
     3601 catcgctgtt acttcaccac cttgagcaac tgcccaagcg tttggaagtg agccagagac
     3661 aagcatcata ccaagaacga taccaacggc aggatttcca ccaaatacac ggaaggttga
     3721 ccacacaacc aaacctggca agatgatgaa ggctgtatct gtcaagattt gtgtgtaagt
     3781 tgcaaagtca cctggaagtg gcatttcaag agcgttgaaa agaccacgca cacccatgaa
     3841 gagacctgtc gctacgataa ctgggatgat tggaacgaaa acatcaccaa aagtacggat
     3901 agcacgttgg aaccagttcc cttgtttagc aacttctgct ttcatgtcat ccttagatga
     3961 tgttggtaat ccaagtacaa caacttcatc gtacattttg ttaactgtac ctgtaccaaa
     4021 gataatttgg tattgccctg agttaaagaa agcaccttga actttttcca agttctcaat
     4081 cacttcttta ttgattttct cttcatcttt gaccatgaca cgtagacgtg tcgcacagtg
     4141 ggcaacacta ttgacatttt cacgtccgcc caaggcatcg atgacttttt ttgcaatttc
     4201 ctgattgttc atttgcaaaa atctccttat ataacatttt gttcttgttt gaaagcgatt
     4261 ttattcgccc ttacgactat tattttatca tgttttaaaa atatgtcaag cgttttgcag
     4321 aaaaaaatat tctatctact tagtgggcaa gctttagatt gattgttttt gactgtttca
     4381 tcaggaattt cttgaaaaat
//
LOCUS       DAVMHG010000138         4377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMHG010000138 DAVMHG010000000
VERSION     DAVMHG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.140"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(310..861)
                     /locus_tag="VYR08_001837"
     CDS             complement(310..861)
                     /locus_tag="VYR08_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467320.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1211..2035)
                     /gene="nadE"
                     /locus_tag="VYR08_001838"
     CDS             complement(1211..2035)
                     /gene="nadE"
                     /locus_tag="VYR08_001838"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV3467321.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2032..3492)
                     /locus_tag="VYR08_001839"
     CDS             complement(2032..3492)
                     /locus_tag="VYR08_001839"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV3467322.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDGDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGNQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3608..4096)
                     /locus_tag="VYR08_001840"
     CDS             complement(3608..4096)
                     /locus_tag="VYR08_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467323.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4086..4295)
                     /locus_tag="VYR08_001841"
     CDS             complement(4086..4295)
                     /locus_tag="VYR08_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467324.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1252 a         1031 c          817 g         1277 t
ORIGIN      
        1 atgaataaaa ttggttgaat ttaattccct aactcgatag aggattctat tttaaattta
       61 acaacagcta ggattacact ataaaaagta cattaccacc tgttcggctg gagaatctag
      121 tcgattagct ccctcttagt tacaagctta aagcgatttt ttaactaata ctaaaccaga
      181 ttaaagaagg aactagaaca tctgtcttgt actaatcaat atgctatttt aaagtttcaa
      241 ataaacttgg aatgctaatt attatattta caataaatag ttgacttcga tagaaacaga
      301 aaatagaatt caacattctt ccctacttat accataataa ataagatccg caagatagcc
      361 ttttctctct acaccattaa caatggtttg tagataggac attccagcct tttccatgac
      421 acgacctgaa gctggattga gactggcata acgtgctctg accttttgaa aacctgcttg
      481 agtaaaacaa aagtccaaga cagctttcaa ggcctctgtc atcataccat gtccccagta
      541 aaccttgcct agcacatagc cgatttcaca gcttaaatca gcctcgtcta tcttaacgat
      601 gctgatatct cctatcactt gctctgggtt ttcttttaga caaatggccc atttgtaata
      661 gttgagatta gtataggaag caacccaatt gcgaatcgag tttcgagtga tttcgacatc
      721 aggatgggga tcccaggtaa cataggtcaa attctcagcg gatgaagccc aattttgaaa
      781 catagcttct gcatcactct ccacaaatct tcttaaaatc aaacgttctg tctgtaatat
      841 ttgcgtaccg atagatttca tgatgctacc tcacttcctt atttctatgc tcgtagtaaa
      901 aagactcaac tcagtaacag ctagaaaacg agtttgtctt ctaatttctt gacgcagtcg
      961 ctgcctggtt tgaaatgcgc tctagcaatc tttttcaaac ctagtcatcg ttgcaggggt
     1021 aagacgatga aatcgaactc ttcgagttac cgaatctgcc tcattttctt atttctaaac
     1081 tcggggttca agatcccccg gaccttctct cactttctta tttcaagatg agaggctgaa
     1141 aacctccagt ggaggttttt cttgcttttt aatttcaggg caagaagcta aaaaggtccc
     1201 ccggaccttt ttactcccaa aagtcatcaa atacggtgat gggtaagtgg cgtttgtgtt
     1261 ggcctttgtg ccaccagttt tcaatggtcg cttgagcttc tgggctgatt gttttgcctt
     1321 ctaggtagtc gtcaatctct gcgtaggtga ctccaagtgc gacttcgtca gctaggcctg
     1381 gtttatcttc ttctaggtct gccgttggga ttttttcata aagggctggc tctgcgccaa
     1441 gtttctgcaa gagctgtttt ccttggcgtt tattgaggcg gtaaagaggg agaatatccg
     1501 caccgccgtc accaaacttg gtaaagaaac ctgtgatatt ttccgcggcg tggtctgttc
     1561 caatgaccgc tccgctatgg gaaccagcaa gggcatactg agcaatcata cggcaacgtg
     1621 ccttgatatt ccccttgttg aagtctgaaa caggactacc tgtcgcttca actgcagctg
     1681 tcatggcatc agctgattcc ttgatattca caaccaagct gacatctggc tggatgaagg
     1741 ctagggcttt ttgagcatct gcttcatcag cttgcactcc gtatggcagg cggacagcga
     1801 taaatttgta gctgtcgtct cccgtttcag ctcgcagttc ttccatagct aattgcgcca
     1861 aacgccctgc caaggttgag tcttgtcccc cagaaatccc tagtacaaag gtttttagga
     1921 agggatgttt tttcagatat ctttttaaga aatcaataga acgacggatt tcttcctggg
     1981 catcaatcac tggtttgaca cccagctctt ggataatcgt ttcttgcaaa ctcattcttc
     2041 ttctccttca ccaagggctt ccttgcgcat cttatcaatc aagtccatct tatcttgcca
     2101 tacatcacgc gccaaatcca ctggatagtg ctgcggattg agcacacgct tatactcatc
     2161 ccacaacttg tcaaattctt tacgggcata atcctgaatg tcagtcaaac taggcaagtt
     2221 gtaaactaat attccttctt tgaagatatc caccaagaga ggaacggcat caaaattacg
     2281 aaccgtcttc ttgatgtatg tataggtcgg atggaacatc ttgatttctg tcatgtcgct
     2341 aatatccaca ccatcataag tgatgtagtc accttctgac ttgccttttt cacgactggt
     2401 aatgcgccac acctgcttct tacctggcgt cgacactttt tccgcattat tagacagctt
     2461 aatcgtattg cgcatctgac cagtttcatc ttcgattgca acaatcttgt aaaccgcccc
     2521 aagagctggc tggtcatagg ctgtaatcag cttggtaccc acgccccaga catcaatctt
     2581 ggccttttgc atcttgaggt tgaggatagt attttcgtcc aaatcattag aagcataaat
     2641 cttagcctct gtaaatccag cctcatccag ttgctgacgg actttcttag aaatgtaggc
     2701 aatatcccca gagtcaatcc gcacacccat aaagttaatc tgattaccca gctcacgcgc
     2761 cacctgaatg gcagctggta caccgatgcg aagggtgtca taggtatcca caagaaagac
     2821 acaatttttg tgggtcgcag cgtaagcctt gaaagcttca tagtcattgc cataaacctg
     2881 taccaaggca tgggcatggg ttcccaaaac aggaatgtca aagagcttac ccgcacgcac
     2941 attgctggtt ccattggcgc caccaatcac agctgcgcgt gttccccaga tggccgcatc
     3001 catttcttga gcccgacgtg tcccaaactc catcaagggt tcatcttcga taaccgaacg
     3061 aatacgagct gccttcgtcg ccaccaaggt ctggtagttg acgatgttca aaagagccgt
     3121 ttcgaccaac tgacattggg ctagaggtcc ttccacctgc acaatcggtt cattagcaaa
     3181 aaccaaatcc ccttcttggg cagaacgaac ggtcaactcc aacttgaaat tgcgaaggta
     3241 atccaagaac gccccatgat aaccaagcga ctccaaatag gctatatcac catctgaaaa
     3301 acgcaagtct tcaagataat tcacaattct ttccaaacct gcaaaaaccg catagccgtt
     3361 cttaaaaggc tgttggcgga aatacacctc aaagaccgcc ttcttattgt gaatcccttg
     3421 gtcaaagtaa acctgcatca tgttgatctg gtacaagtcc gtgtgcaatg tcaaactatc
     3481 atctggatac atacttttcc tacttcctta gctagaaacc catgaaaatt ttcaagaact
     3541 ttcatgtatt ccaataaatt agtactatta tatcacattt tagctggatt gagaaaagag
     3601 taacaagcta ttctccactc tccaattcat ccatatcttg ttcaaatttt ttctgagccc
     3661 attcgccata gctcttaaga ccaagattgc caataaagac ccacggaagg taaatgacat
     3721 aagtaatgac ccaagcagac aggtatttaa aattcaaagg attgtgctga taaatttcta
     3781 tgttgaattg ataattctgc aacatcaaaa gagccgtaat agccaaggtt aggaaaaaac
     3841 aacccaaaat cgtaaaatga aaacgactat agtaggtcac tcccagataa cgggcacgat
     3901 tgaaaaagta aaatgtccct atgatgataa cgattagcag catattagaa ttaaaaaggc
     3961 ttggtgctaa tactgaaatg atataagata ggagcgacaa agcaatgcag atatagaaac
     4021 tttcagagcc cgctttattg aacagttgtt cttctctttc gtctagtaat tgataataat
     4081 aaaatctatt tttcatcttc ttcctcccaa aatagttggt ctagggtttt ccctaaacat
     4141 ctgcaaatag actggcagag cgagagactg ggattgtatt ttcccgcctc tatcaaacca
     4201 atagtctggc gtgtcacccc gacagcctct gccagttgac cttgtgttaa atcacgctct
     4261 acccgagcta attttaattt taaattttta gccaccttcg tcctccttat agttttaata
     4321 ctcatctact cttaaaaaat ccaaaaccaa cacaagctat cagctattta ttataac
//
LOCUS       DAVMHG010000139         4236 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMHG010000139 DAVMHG010000000
VERSION     DAVMHG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.141"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..942)
                     /locus_tag="VYR08_001842"
                     /pseudo
     CDS             complement(1..942)
                     /locus_tag="VYR08_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1490..4047
                     /locus_tag="VYR08_001843"
                     /pseudo
     CDS             1490..4047
                     /locus_tag="VYR08_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1296 a          650 c          915 g         1375 t
ORIGIN      
        1 ttcgaaaaat ttctccggtt ccttattctg aaagtgaaac agcaagagtt gatagagctg
       61 atagtgatgt ttcaagtctt gtgaatagct caaaagcttg tctaaaatct ctttattggt
      121 taaatgcata cgaaaagtag ggcgataaaa atgtttatcg ctgagtttac ggctatcctg
      181 ttgaatgagt ttccagtagc gcttgatagc cttgtattca tgggattttc gatgaaactg
      241 attcatgatt tggacacgca cacgactcat agcacggcta agatgttgta caatatgaaa
      301 gcgatcaaga acgattttag cattcgggag tgaaacagtc tgggagactg tttcagcctg
      361 agcctagaaa tttgaaagcg aagctgttta gctaagtcat agtaaggact aaacatatcc
      421 atagtaataa ttttgacgcg acatcggacg gctctatcat atttaagaaa gtgatctcgg
      481 atgacagctt gtgttctacc ctcaagaaca gtgatgatat cgagcttttc aaaatcttgc
      541 gcaataaagc tcatctccat ctcccgattg aaacagtcac tccccggact gtttcaacgt
      601 cccaagacat aatcttaggc agatttctaa aattacactc aaagtggaag tcattgagct
      661 ttcgaatgac agttgaagtt gaaatggcca gctgatgggc aatatcggtc atagaaatct
      721 tttcaattaa cttttgcgca atcttttggt tgataatacg aggaatttgg tgatttttct
      781 tgacgataga agtttcagcg accatcattt tcaagcaatg atagcactta aaacgacgtt
      841 ttctaaggag aattctagta ggcataccag tcgtttcaag gtaaggaatt ttatagggtc
      901 tttaatgtct agtaattttg tgataaaatg taattgttcc atatgattct ttctaatgag
      961 ttgttttgtc gcttttcatt atagatctta tgggactttt tttctaccca aaataggctc
     1021 cataatatcc atagggaatt tacccactac aaatattata gagcccaaag ttttaggtcg
     1081 cttgataata tgcgtttttt gaattttata gactgctcgt ttaaactcta tttacttcgt
     1141 accttctgga gcgagacgga atattagtca catacaaaat gagtactatt aggattttat
     1201 tttcatgtac aatttcagcc agtcttgtta taatcagcct ataggaatca aggaggtgac
     1261 tcttatggct gtttttgtgt ctttggatgg aattgtggta gaagtccttg atgtcttttc
     1321 ttcttttaat ggggatagtg agtttttctt gtgtatagca ttttgaatct ggaataggac
     1381 gccatgactg ctaaaagatt tctataaatt aatttgattt ttctaatcaa tttgttcata
     1441 tcttatttca ttccactata aacgtcttaa agacaagagt cagtttgtta tggaacgctc
     1501 tcagttcgag gagatgttcc aacttcaaag tagtcgcttg acgacgcaag aaaaattaca
     1561 attgtttacc tctgtgtttg ctggccgtta tgatgtttat gctaagaatt ttattaatga
     1621 acaagggaaa attcagtatt ttccttccta tgattatggt tggaagcagt tgccacctga
     1681 aaaacggagt ttccagacat tgacgaactc cgttttgaaa tctcattttc gtggggaggc
     1741 agctatcggt atctttccta tgcacttaga tgatagctgt tattttttgg tactggattt
     1801 ggatgaagga gattggaaag aagctggttt aaccattcga agaatagcca gggaacgcca
     1861 gatggaagcc catttagaga tttctcgttc gggttacgga ctccatattt ggttcttctt
     1921 tgaggaagcg attctgagtc gaaaggctcg cctgtttgga aagaaactat tagaattggt
     1981 tatgcaggaa agtatgcaac tgtcctttga ttcttttgat cgcatgtttc caaatcagga
     2041 tgttcttcct aaaggtggat ttgataattt aatttccctt ccttttcaag gagaagctta
     2101 tcatcaaggg cgaacggtct ttgtggatga acattttcag ccttatgaag atcaatggag
     2161 gtatctacaa gaaattcaga agatttcaac tgctaaagtg gcactgttaa tccaagagga
     2221 gttaggcaag caagaattgg ataaggagtt gaaggtcgtt ttatccaata tgatccaact
     2281 taaaaaatcg tctgtgacac ccaagacact gtttttcttg aaaaatatgg cttcattttc
     2341 taatcccgaa ttttatttaa agcaggctat gcgacagcca acctatcaaa ttcctgagcg
     2401 aatgtattta tttggagaat ccgattatta tttatggctg ccaagaggct tgttagatcc
     2461 attgcaagat aaatttaagc aggtagttgt ggaggatagg agaaaagtac aaaggtctat
     2521 tagagtagca tttaagggag agcttacttt ggagcaagaa ttagccctgt cagatatgaa
     2581 ttctaaagaa aatggtttac ttcatgcggg acaggttttg ggaagaccgt tttaggtgct
     2641 gctcttatct ctgaacggaa aacaaaaaca attattctag tccataataa gcaactctta
     2701 gaccaatggc tagatcgctt aaactgcttt ttgactttcg aagaggagga ggctatccgt
     2761 tatacggcat caggtcgtga aaaggtaatc ggctatgttg gacagtacgg tgggactaag
     2821 aaatggctga gtaaactggt tgatgtcgtt atgattcaat ctctatttaa gttggaaaat
     2881 agtcaaagtc ttttggatga gtatgagatg atgattgtgg atgagtgtca tcatgtctct
     2941 gccttgatgt ttgaaaaagt tgttgctcag tttagaggga agtatcttta cggtttgacg
     3001 gctacgcctg agcgtaagaa tggtcatgag cctattgttt ttcagagaat tggtgagata
     3061 ctccatactg ctgataagag ggaaacggat tttaaacggc aattgcaatt aagattcact
     3121 tcttttggtc atttggagat tgaaaagacc aaagcaagta attttataca gcttagtgat
     3181 tggattgcta ctgactcagc gaggaatcag ttgattctca aggatattct agcccaagtg
     3241 gcagaaggac ggaatatctt ggttttagtt aatcgaattc aacagataga tgtctttgaa
     3301 aagttattga aagagaaaga ggttgatgac tgttacatta ttagcggaaa aaccaaagtc
     3361 cgagaaagaa cgagtttact ggagacgtta gaacagttag ataaagggtt tgttttgttg
     3421 tctactggaa aatacattgg cgaaggtttt gacttacctc agttggacac gcttatcttg
     3481 gcagcaccct tttcttggaa aaataattta attcagtatg caggtcggat tcatagaaac
     3541 tacaaggata agtctttggt gcgtattttc gattatgtgg atattcatgt tccttattta
     3601 gaaaagatgt ttcagaaacg acaagtagct tatcgaaaga tggattatcg tgtcatcgag
     3661 ggtgaggaga aacaattcgt ttatgttgat agtagatatg agaaggtgtt gagagaggac
     3721 ttagcagggg aaagacagga atgtctgctt attttacctt atgtgcacca gacaaaactg
     3781 atgaattttc taaaagaatt taggattagt caaattgaga tatgtatacc agagacggtt
     3841 gcaaataaag catggctaga ccagttgaag agccagaaaa ttaaagtgtc ttttactcaa
     3901 tcaaaaatag taacgcctat tcttttggtg aataagacta ttgtttggta tggtgcaatg
     3961 ccattattag ggaaggtaga tgagatgacc atattacgtt tggaatcagc tagtatagtt
     4021 tctgaactag tggcaggttt acgatagaga aaatttttaa aaatttctat gtatgatttt
     4081 catttcttta gtgagactgt tgccattatc acattcgaat cacacaaaat aaaaaaattt
     4141 ttataagtac ttgacaaata gattgaaata tcataaaata aaaacggtta cagagttatt
     4201 aattatttaa gcttcatgtc accattaaaa attgaa
//
LOCUS       DAVMHG010000140         4170 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMHG010000140 DAVMHG010000000
VERSION     DAVMHG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.142"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            68..613
                     /locus_tag="VYR08_001844"
     CDS             68..613
                     /locus_tag="VYR08_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467325.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            622..1161
                     /locus_tag="VYR08_001845"
     CDS             622..1161
                     /locus_tag="VYR08_001845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467326.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            1197..1742
                     /locus_tag="VYR08_001846"
     CDS             1197..1742
                     /locus_tag="VYR08_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467327.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            1899..2993
                     /locus_tag="VYR08_001847"
     CDS             1899..2993
                     /locus_tag="VYR08_001847"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV3467328.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            3001..4167
                     /locus_tag="VYR08_001848"
     CDS             3001..4167
                     /locus_tag="VYR08_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV3467329.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHVRINIAMPKSLLQEVCQRIVATFAKR"
BASE COUNT         1223 a          721 c          929 g         1297 t
ORIGIN      
        1 tattagattt tatcctcaaa ttgagttatt cccgacttta tccgttgaaa tattataagg
       61 agtatttatg aaattaaaat tattaagagt agatactaag gtgattatgg ggagtttctt
      121 acttgttctg tctagtctac ttgctttgtt gcttcccctt atcttaaagg gtttaataga
      181 tgggagttct attgaaaata taggttccaa agtatttcaa tcgtttttga tttttattgg
      241 tcaagccttg ttttcttcta ttggttacta tctgtttagt caatcgggtg aaaaaaagat
      301 agcaaaaatc aggaaaaaag tgatagaggg gttgatttat gcagagaaat ccttctttga
      361 taagagccaa agtggggagt tgacttctgc cattgtcaat gacatgagtg tcattcgtga
      421 gtttttaatt acgactttcc caaatattat tctgagttta gttatggtac ttggttccat
      481 tgtagtctta tttagtcttg attggaatct ttctctactt ttattcatca ctcttccttg
      541 tatgatgttt attatcttgc ccctttccaa tatcagtgaa aagtatagtc gtcgtttaca
      601 ggaggaaatt taacagggca attgactgaa aagattcaag aacatgaatt aattaagact
      661 aaccaagcag agaaaagtgt acaggatgtt ttggataatt gtattgaaag ggtacaaaac
      721 aattcactga aatcagatag ggttacttct tttgagaccc cgtttgctct cttatttatc
      781 tttgcgacta tagctgtgat gctaacctat gggggttatc gggtcagcgc aggatatata
      841 tctgtgggaa ccttggtttc gtttttgatt tacctctttc aattacttaa tcctattagt
      901 aatatagcta attttgtaac tgtttattct aggagcaagg gatcttcagt tgcactggat
      961 aacttgcttg cagttcctaa agaaaaattt gagggaggaa aatcggtatc aggacaaggg
     1021 ttgaatttta accatgtcta ttttggttat gatgaaaatc gacctgtctt aaaggatatt
     1081 acttgttcaa ttttcaaggg gcaaaaattg cttttgttgg accatctgga tcaggaaaat
     1141 caacgattgt gcgtttgtta gagcggtttt ataaaccgct ttcaggagat attctaatgg
     1201 agcaatcaag tatatatgat tttaacttaa aagaatggag aagtaaaatc gcttgggttt
     1261 cacaaaataa tgcagtctta tctggcagta ttcgtgacaa tctttgtctc ggtttgaatc
     1321 gcttagtaac tgatgatgaa ttgatgaaag tgctagactt agtatcacta ggtgatgaga
     1381 ttcgctccat gaaagaggga ctagatactg aagttggtga acgcggacga ttcttgtcag
     1441 gggggcaaag ccaaagactt caaatagcta gaacctactt aaaagatgct gaaattctta
     1501 tatttgatga agctactgct aatcttgatg cggattctga gtatgcgatt atcagtagcc
     1561 tctattctgt attaaaggag aagacggttg tgattatagc gcatagtttg tcaacggtaa
     1621 aagatgtgga ttgtattttc ttcttagagg aggggaaaat cactggctca ggaactcata
     1681 aggaactact ggaaaatcat gagcgttatg ctcgttttgt gcaggagcaa atgatagagt
     1741 gaagtgtctt ttgagattca ccattttata gtctattaaa gggagcagga aaaactccct
     1801 ttttatatag tttgaaacta taactagctc ttgaaaagaa gaaaatgagt tgatgaaaat
     1861 aagtggtaca atagttacta tagatttgga ggtattgtat gagcaaggaa ttacacatta
     1921 acacaatttt ggcccaggcg ggtattaagt cagatgaagc gacaggtgca ttggtgacac
     1981 cgcttcattt ttcaacgacc tatcagcatc cagagtttgg tcgatctact gggtttgact
     2041 atacgcgcac taaaaatcca actcgtagta aggctgagga agtcttggcg actattgagt
     2101 cagcagacta tgccttagcg actagctcag ggatgtcagc tattgtactg gcctttagcg
     2161 gctttccagt aggaagtaag gtcttggcag tgcgtgatct ttacggcggt tcttttcgct
     2221 ggtttaacca agtggagcag gaaggtcgtt tccattttac ctatgccaat acagaagaag
     2281 agttgattgc cgagttagaa aaggatgtgg atgttctcta tatcgaaacc ccaaccaatc
     2341 ccttgatgtt ggaatttgat atcgaaaaac tagcaaaatt ggctcatgct aagggtgcca
     2401 aagtggtggt ggacaatacc ttctatagcc ctatctacca acgtccgatt gaagatggag
     2461 cagatatcgt tctccattca gcaaccaagt atctagcagg ccacaatgat gtcttggctg
     2521 gagtggttgt gaccaatagt ttagaactat acgagaagct tttttacaat cttaatacaa
     2581 caggggcagt cttgtctcca tttgacagct accagttgct tcgtggtctc aagaccttgt
     2641 ctcttcgtat ggagcgctca acagctaacg cccaagaagt ggttgccttt ttgaaggatt
     2701 ctccagcagt taaggaagtt ctctacactg gtcgtggagg catgatttcc tttaaagtag
     2761 ccgatgaaac acgcattcct catattttga acagtctcaa ggtcttctct tttgcggaaa
     2821 gtttgggtgg agtggaaagt cttattactt atccaacgac tcaaactcat gctgatattc
     2881 cagcagaggt acgccattct tatggtttga cagatgacct cttgcgtttg tctattggga
     2941 ttgaggatgc tagagatttg attgcagatt tgcgccaagc catagaagga taagacaaag
     3001 atgggaaaat atgattttac aagcctgccc aaccgtttag ggcaccatac ctataaatgg
     3061 aaagaaacag aaacggatag tgaagttcta ccagcttgga tagcggatat ggactttgtg
     3121 gtcttgcctg aaatccgcca agccgtgcaa acttacgcag accaactggt ttatggttat
     3181 acctatgcca gtgaagactt aattaaggaa gttcaaaagt gggaagctac acaatacggt
     3241 tacaactttg acaaagaggc tcttgtcttt atcgagggtg tggtaccagc catctcaaca
     3301 gctattcaaa cctttacaaa agaaggcgag gcggttttaa ttaacacgcc tgtctaccca
     3361 ccctttgctc gcagtgtcaa gttgaataat cgtagattga ttactaattc cttagtggaa
     3421 aaggatggtc tgtttgagat tgactttgac caacttgaaa aggatttggt ggaagaggag
     3481 gttaaactct atattctttg caaccctcac aatcctggtg gacgtgtttg ggaaaaagaa
     3541 gtgttggaga agattggcca actctgccaa aaacacggtg ttttgttagt ttcggatgag
     3601 attcaccaag atttgaccct ctttggtcac aaacaccagt ctttcaatac catcaatcct
     3661 gccttcaaaa attttgctat cgtcttgagc agtgccacta aaacatttaa tattgctgga
     3721 acaaaaaatt cctatgcagt cattgaaaat cctaagttga gactagcttt ccagaaacgc
     3781 ctgttggcca ataatcagca tgaaatttca ggcttgggtt atttggcgac agaagctgcc
     3841 tatcgatacg gtaaagattg gctagaggaa ctcaagcaag tctttgaaga ccacatcaat
     3901 tatgtggtgg atctatttgg aaaagagact aaaatcaagg tcatgaaacc gcaaggtacc
     3961 tacttgattt ggcttgactt ttcagcttat gacctgactg atgaaacatt gcaagagttg
     4021 ttgagaaatg aagccaaggt tatcctcaac cgtggtttgg attttggaga ggaaggaagt
     4081 ctccatgtcc gcatcaatat agctatgccc aaatctctgt tgcaagaagt ctgtcagcgg
     4141 attgtggcta cttttgccaa acgttaaaaa
//
LOCUS       DAVMHG010000141         4118 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMHG010000141 DAVMHG010000000
VERSION     DAVMHG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.143"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..117
                     /locus_tag="VYR08_001849"
     CDS             <1..117
                     /locus_tag="VYR08_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV3467330.1"
                     /translation="QKILDLRKKYHLQMQVERYIEDDSGMPESDKSFIDTGN"
     gene            307..1743
                     /locus_tag="VYR08_001850"
     CDS             307..1743
                     /locus_tag="VYR08_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV3467331.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLCDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            1795..2034
                     /locus_tag="VYR08_001851"
     CDS             1795..2034
                     /locus_tag="VYR08_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV3467332.1"
                     /translation="MIGSIAAILTTFAFLPQVFRIVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2164..2478
                     /locus_tag="VYR08_001852"
     CDS             2164..2478
                     /locus_tag="VYR08_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV3467333.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2596..>4118
                     /locus_tag="VYR08_001853"
     CDS             2596..>4118
                     /locus_tag="VYR08_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV3467334.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDATERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKIN"
BASE COUNT         1411 a          515 c          830 g         1362 t
ORIGIN      
        1 cagaaaatct tagatttaag aaaaaaatat cacttgcaga tgcaagttga acgttatata
       61 gaagatgatt ctgggatgcc agaaagcgat aagtcattta tagacacagg gaattagaat
      121 tattgtgaga gtttttgtag tttgaaagta ttgaaaatcg taaagatatc attacgagaa
      181 tacaagaaga tattgttcta tttccattaa aaattacttc tttccttaac aataaggaaa
      241 aagaatgcgt tttcatcttt tggtttaagg tttataataa gagtatcaaa gtcaggagga
      301 aaaaagatga ctacttttaa agatagattt ttatggggtg gtgctgttgc tgctcatcaa
      361 cttgaaggcg gatggcaaga aggtggcaaa ggaattagtg ttgctgatgt tatgactgct
      421 ggtcgtcatg gagtagctcg tgaaattact tcgggagttt tagagggtaa atattatcca
      481 aatcatgagg cgatagattt ttatcatcgt tataaagaag acatagcact ttttgctgaa
      541 atgggattca agtgcttccg tacctctctt gcatggacac gtatctttcc aaaaggtgat
      601 gagttagagc cgaatgaaga aggattacag ttttatgata atctttgtga tgaatgctta
      661 aagaatggta ttgaacctgt catcactcta tctcattttg aaatgcctta tcacttagtg
      721 accgaatatg gtggttggaa aaataggaaa ttgattgatt tctttgctcg ttttgcagaa
      781 gtcgtattta aacgttacaa agataaggtt aaatattgga tgactttcaa tgaaatcaat
      841 aatcaagcga attatcagga agattttgca ccatttacta actcaggtat tgtatatgag
      901 gaaggtgata atagagaagc aattatgtat caagcagcac attacgaatt agttgcttct
      961 gcacgagctg taaaaattgg tcatgagatt aatccagatt ttcaaatagg ttgtatgatt
     1021 gcgatgtgtc caatttatcc agttacttgc aatcctaagg atatcttaat ggcaatgaaa
     1081 gctatgcaga agcgttatta ttttgctgat gtgcatgttt taggtaaata tcctgagcat
     1141 attttcaagt attgggaacg aaaaggtatt tcagttgatt ttactgacca ggataaagaa
     1201 gatttacttg gtgggactgt agattacatt ggtttcagtt actatatgtc ctttgctata
     1261 gactctcatc gtgaaaataa tccttatttt gattatcttg aaacagaaga tttagtgaaa
     1321 aataattatg ttaaggcttc tgaatgggag tggcaaattg atccagaagg tttacgttat
     1381 gcgttaaatt ggtttacaga ccactatcac ttaccactct ttattgttga aaatggtttt
     1441 ggagctatag atcaagttgc agcagatggt atggtacatg atgattatag aattgaatat
     1501 ctaggtgccc atattcgtga aatgaaaaag gctgtagttg aagatggtgt tgatttaatg
     1561 ggttatactc catggggatg tattgatttg gtttcagctg gtaccggtga aatgcggaaa
     1621 cgttatggtt ttatttatgt agataaagat gacaatggga agggaagtta taatcgttcc
     1681 ccgaaaaaat cttttggctg gtataaggaa gttatttcat ctaacggtga atcagtagaa
     1741 tagtataaac aaactgagat tattctcagt ttgtttagca tattgggatg acatatgatt
     1801 ggaagtattg cagcaatttt aacaacattt gcatttttac cacaagtttt tcgtatagtt
     1861 aagacaaagg acacaggatc tattgctcta ggtatgtatg ttatgcaagt tattggtatt
     1921 gcattgtggc ttgctcatgg tattcgtata ggagacttgc ctttgatttt agcaaatagt
     1981 gtttcatttc tactctcagg aataatttta ttttataaat tgaagtataa ataatttttt
     2041 tccatatcat ttaggaaaat gagggaattg tcattggaaa cgctttattt tataataaaa
     2101 atagataaca aataagccaa cttagcacag ctttgattgg atttaagaaa aggagaaaat
     2161 attatggcaa aagctttaat tatttgtgca gcaggaatgt cttcatcact tatggcacag
     2221 aaaacaacag agtttctaaa aggaaaaggg aaagatattg tagttgatgc tattagtgct
     2281 actgaaggag ggaaagctat cgcagcagca gcttttgatt tgtatcttgt cagccctcaa
     2341 acaaaaatgt actttaagca atttgaagag gctggttcta aagcaggaaa accagttgta
     2401 caaatacctc ctcaagcata tattcctatt ccaatgggaa ttgaaaaaat ggctaatttg
     2461 attcttgaga atatttgatg aacacatgaa atgtcaataa atatttaggt taagataaat
     2521 tgttttgagt ttgatataaa ctgatttaag tgtataatat gcttcaatat atgaaaacat
     2581 acgttttagg agggaatgaa tgtgttaaac attaaagaga aagatatcct tcaattttta
     2641 ataaaaaata aggaacgttt tgttacaagc aaagaactgg cagaatattt atcctgttca
     2701 gatagaactg tccgtaatgt actgaaatta attgagaaga caatgattat tcaaggagtc
     2761 cggttgattt caaaacaagg tcaagggtat caaatatttt ttgagaatca aggcgcttac
     2821 caggagttta gacaaactta tgaactggaa gaagactata cgaaaactgc ggtttcaaaa
     2881 ggtgatgatc gtttagtatt tatcttaaat aaattattat ttgagcaagt tccagttctt
     2941 tttgatgatt tagctgatga actttacgtt agccgttcaa cattatctca tgatttcaga
     3001 aagatacgag tgatgttatc agagtataat ttgtccattg agagtagggc gaataaagga
     3061 gtttatgtct caggcgagga acgtgataaa cgacgtttta tcataaatta tttcctagag
     3121 aatcaatttt ttaaaacaat acattgctat gtaaaattta acttttttga tcaaactgtt
     3181 ccattagaag aatttgctag aattgttttg gatgaatgcc aagaggccaa cctaaaacta
     3241 tctgattttg tactacagaa cttagtagtt cacatagcat tgtctgtgat tcgtttaaaa
     3301 tccggatttg aaataaaaaa tatagactgt cagatgactg atgatgcaac agagagaaag
     3361 gttgcccaaa gaattctttc taaagtaaga gaagtaacaa atcaggaatt tccagttcag
     3421 gaaatagact acattacttt gcatttgtta gctaagagtc aacaatgtca aaaaaatcag
     3481 aagaatattt ctgaagaagt tttaaaaaaa tctttattta aaacatttca gaatttagga
     3541 ttagatgata tgtataactt ttcttcagat ttccaactaa ttgaaggatt gataactcat
     3601 ctaatgacac tacaagtacg tttagaaagc cggattacct taaataaccc tttagtagat
     3661 gaaattaaac agaattatag tgatatattc tttatgacta gagaaattct tgcaaatatg
     3721 gacaagttct tggagtggac aatatcagat gatgaagtag catatgtaag tcttcacttt
     3781 ttagctgcta tggaacgtag caaagagagt actaagttta atattcttgc tatttgtgca
     3841 acaggatttg gtgcagccca aatgttaaga aatcgtttgg aaacggagtt tgggaagcga
     3901 gtagaagttg ttgatgtaat aggctattat gaattgaatc aagaaaaact taaaggaata
     3961 gattttatag tgtcagctgt tgatttatca aatctatatt ttcaaatccc agtttttaaa
     4021 gttagtgtgt ttcttaagag tgatgagatg gaaatgatta gaaaagctat ggatcagatg
     4081 caagtttcta gttatgtaca atcatcaaag attaataa
//
LOCUS       DAVMHG010000142         3913 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMHG010000142 DAVMHG010000000
VERSION     DAVMHG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.144"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1228)
                     /locus_tag="VYR08_001854"
     CDS             complement(<1..1228)
                     /locus_tag="VYR08_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV3467335.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAI"
     gene            complement(1228..1809)
                     /locus_tag="VYR08_001855"
     CDS             complement(1228..1809)
                     /locus_tag="VYR08_001855"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV3467336.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(2209..2304)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2687..2926)
                     /locus_tag="VYR08_001856"
     CDS             complement(2687..2926)
                     /locus_tag="VYR08_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3467337.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(2926..3069)
                     /locus_tag="VYR08_001857"
     CDS             complement(2926..3069)
                     /locus_tag="VYR08_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV3467338.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(3081..3908)
                     /gene="xth"
                     /locus_tag="VYR08_001858"
     CDS             complement(3081..3908)
                     /gene="xth"
                     /locus_tag="VYR08_001858"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV3467339.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTITFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
BASE COUNT         1188 a          777 c          882 g         1066 t
ORIGIN      
        1 caatagcgag taggctggct acgacgattc cgtttgagaa gaacatttgg aaggctgttg
       61 gcatgctgac aaagagatta ctgttgttga gaccgacacc tgcagcgatt gaaacagctg
      121 cgataaggaa gttgtgttca ttgttagcaa agtcaactcg ggcgaggatt tgcatccctt
      181 gaatagatac aaaaccaaac atcaccagca tggcaccgcc gaggacggag cttggaatga
      241 tttgggcaag ggcaccaaac ttaggaagca gtccaaggag aaccaggaaa ccagctgcgt
      301 agtagattgg caggcgtttt ttgatgcctg acaatttaac caaaccaacg ttttgtgaaa
      361 atccggtgta agggaaggtg ttaaagattc ctccgagaag tacggccaaa ccttctgcgc
      421 ggtatccgtt gcgaaggcgc gtgctgtcga ttggatcctt tgtgatatca gacaaggcta
      481 gataaacacc agttgactca accatagaca ccgttgcgat gatacacatc atgacaatag
      541 atgagatttc aaaggttggc atcccaaagt agagtggaat tgggacatgg acaagtggag
      601 ctaccgcaac aggagagaag tccaccaagc ccatagtagc agcaatggca gttccaacaa
      661 ccagaccaat caaaatagag atagacttga taaatccttt ggtaaagata ttgatcaaga
      721 ggataatcag aacagtaata gctgcaagca agagactttg accagttggc tctggaacgt
      781 tatttcccat atttccaata gcgacaggga tcaaggttaa accaatcgtg gtaataacag
      841 atcctgttac gatagatggg aagagattgg ctacttttga gaagatgcct gaaacaagaa
      901 ccacgtaaat cccagatgcg ataagggcac caaacatagc gccactacca tggctttgcc
      961 caatcataat caagggagcg accgactgga atgcaactcc aagaacgact gggagtccaa
     1021 tcccaaagta tttgttgagt tggagttgga ggaaggttgc caccccacac atgaagatat
     1081 ctgtagaaat caggtaggtc aactgctcag ctgaatagcc aagggctgtc gcaatcatga
     1141 tgggaaccag gatagatcct gagtacatgg ctagtaagtg ctgcaagccc agaacggctg
     1201 cttgcgagtg tttttcttga gtttgcatta gagatctgcc tccttaaata cgacctgacc
     1261 attttcaaaa cgatccaaac gagcaagtga taggacaggg tagcctgctt tttcaagcaa
     1321 atcacgacca tcttggaagg atttctcaat cacgataccg atagcttgga ctgtggcacc
     1381 ggcctgttcg atgatttgaa tcaagccttt agcagcttgg ccattagcaa ggaaatcgtc
     1441 gataatcaaa accttgtcct ctggtgagag gaattttcca gcgatagaaa cagtgctggt
     1501 cacctgcttg gtaaaggagt agacttgagc agttaagatg ccttcgttca tggtgatgtt
     1561 cttagctttt ttggcgaaaa tcatgggaac gtttaaggct tcagctgtaa aaacggctgg
     1621 ggcaataccc gacgcttcaa tggttacgac cttggtaatg ccagcagcag caaatttttc
     1681 cgcaaaaacc ttaccaatct ctcgcatcaa gctaaagtca acttggtggg ttaaaaagga
     1741 atctaccttg aggatgttat cacccaagat atgcccatcc ttgaggatgc gctcttctaa
     1801 taatttcata agacctccta aagtctaaaa gttaatttac ttgttgttta aatgtttcta
     1861 tagtgatccc ttttgctaat attatatatt tgataaaact attacgagcg aagcgagtct
     1921 tatcaaatat ttcccgttgt agtggtatca tagacaataa tcttgttatt gtctatgacg
     1981 ggatttttga gagtaaaata gttcggggaa ctattttagc ctaagcctag aaatgaaaga
     2041 gctaggggct caaaaattag ggatgaaatt ccctggattc ctgaaattat tcacaggata
     2101 atttcacctc ccgtccgcac taattaaggg aaatattaaa aatattcaaa aaagacctac
     2161 ttaatctcta agtaagtccc ccaaataggc atggcaaaaa cggccatatc tcactgctga
     2221 cttacttatt gttaggtgtt ccggcacctt gtagaaacgt cgtgccaatt cacgacataa
     2281 acaagtaaaa cgatattcaa ttttaaatag gcttgagcca atgtttttat tttacactaa
     2341 ataactttaa aaatcaacta ttttgttagt gtttcggttt aaaaaacgaa caaacagaag
     2401 agagggtgaa taaaaactcc attgtaagct aacatttata ctaaatgaaa atcaaagagc
     2461 aaactaggaa gctatccaca acctcaaaac actgttttga ggttgtggat agaattgaca
     2521 gagtcagtat catataccta cggtaaggcg acgttgacgt ggcttgaaga gattttcgaa
     2581 gagtattaga agatttttcc atcataaaag gcatactatc aagtttttag acacctgaca
     2641 atatgccttt ttctaacttt aaagactttt cccaattttt attattctac tcgctaaatc
     2701 ttaaaaaata gccatctgga tccaaaactg caaatttatg aggatagata tagggatcac
     2761 tgacacgaaa ctttcttttg gtcaagggac gataaatagg atagtttgcc tccatcactt
     2821 tttgatagag ttttggaaca tcctttatgc caaaggagag attgactcca cgaccaaagg
     2881 ggtaggtcag ttcagctagt tgatcctttg ttccctcctc taacattagt tgacactctt
     2941 caagagaaag agaaagtttt cttctggacg ttggtattca atcctaaaac ccagtaaacc
     3001 acagtagaag gaccgggact gttcgatatt cgatacaaga aactcgggaa tgaccgcatt
     3061 gtagtccata tagaaaatcc ttacaagtca atttccaaga caatcggtgt atggtcttgg
     3121 cgagcacctg agtcaatcat atcagattta gtgaccttgt cagcgatacg gttacttgtg
     3181 agccagtagt cgattctcca gcctgtattg ttgattttag aagttttgct gcgttgtgcc
     3241 caccaagtgt agcgttcagg aacatcgcca tgaacatggc ggaaggtatc agtaaatcca
     3301 gttgccaata ggttggtaaa tccagcacgt tcctcgtcag taaatccagg tgaacggcgg
     3361 ttgctagcag gatttgcaag gtcgatttca ttgtgggcta cgttgtagtc accggtcgca
     3421 aggactggtt tttctttgtc tagttcagcc aaatactccg catatttgac atcccagact
     3481 tggcgttctt ccaagcgttt aagaccatcg ccagcatttg gtgtgtaaac ttgggtcacg
     3541 aaaaatgcat caaattctag agtgatgatg cggccttcca agtccatggt agaaggggca
     3601 ccgatttctg ggaaagtgat agtaggtgta agttctttct tataaaggaa catggttcca
     3661 gcatagcctt tacgggcagg ctcttgggaa gagcgccacg tgttttcgta gcctgggaag
     3721 agttcttcta aaatttccac gtgtttcttt gtaggtcctt tggcagaaag cttggtttct
     3781 tggatagcaa tgatatcagc attttcagcg accaaggttt gtaggacttc ttgggacaat
     3841 ttggcacgag ctgagtcact agttagggca gcgtttaggg aatcaatatt ccatgagata
     3901 agtttcataa agt
//
LOCUS       DAVMHG010000143         3883 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVMHG010000143 DAVMHG010000000
VERSION     DAVMHG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.145"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..429
                     /locus_tag="VYR08_001859"
     CDS             <1..429
                     /locus_tag="VYR08_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3467340.1"
                     /translation="DHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSA
                     DNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAM
                     ETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            651..3770
                     /gene="phtE"
                     /locus_tag="VYR08_001860"
     CDS             651..3770
                     /gene="phtE"
                     /locus_tag="VYR08_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV3467341.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDLNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNSSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSIGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1452 a          629 c          757 g         1045 t
ORIGIN      
        1 gaccatgttc gtaaaaataa ggcagaccaa gatagtaaac ctgatgaaga taagggacat
       61 gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca cgctggttta
      121 aatccttcag cagataatct ttataaacca agcactgata cggaagagac agaggaagaa
      181 gctgaagata ctacagatga ggctgaaatt cctcaagtag agcattctgt tattaacgct
      241 aagatagcag atgcggaggc cttgctagaa aaagtaacag atcctagtat tagacaaaat
      301 gctatggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa agataataac
      361 actatttcag cagaagtaga tagtctcttg gctttgttaa aaaaaagtca accggctcct
      421 atacagtagt aaaatgaatg gagcatattt tatggagaag taacctttcg tgttacttct
      481 cttttttaga aaaacgtaac agaaacttga caaaagtaat tttaaatagt aaactattta
      541 ctggttaatt aaatggttaa ttaaatggtt aaataaccgg tttagaaaac tatttaataa
      601 agtaaaagaa gttgagaaaa aacttcatca tttattgaaa tgagggattt atgaaattta
      661 gtaaaaaata tatagcagct ggatcagctg ttatcgtatc cttgagtcta tgtgcctatg
      721 cactaaacca gcatcgttcg caggaaaata aggacaataa tcgtgtctct tatgtggatg
      781 gcagccagtc aagtcagaaa agtgaaaact tgacaccaga ccaggttagc cagaaagaag
      841 gaattcaggc tgagcaaatt gtaatcaaaa ttacagatca gggctatgta acgtcacacg
      901 gtgaccacta tcattactat aatgggaaag ttccttatga tgccctcttt agtgaagaac
      961 tcttgatgaa ggatctaaac tatcaactta aagacgctga tattgtcaat gaagtcaagg
     1021 gtggttatat catcaaggtc gatggaaaat attatgtcta cctgaaagat gcagctcatg
     1081 ctgataatgt tcgaactaaa gatgaaatca atcgtcaaaa acaagaacat gtcaaagata
     1141 atgagaaggt taactctaat gttgctgtag caaggtctca gggacgatat acgacaaatg
     1201 atggttatgt ctttaatcca gctgatatta tcgaagatac gggtaatgct tatatcgttc
     1261 ctcatggagg tcactatcac tacattccca aaagcgattt atctgctagt gaattagcag
     1321 cagctaaagc acatctggct ggaaaaaata tgcaaccgag tcagttaagc tattcttcaa
     1381 cagctagtga caataacacg caatctgtag caaaaggatc aactagcaag ccagcaaata
     1441 aatctgaaaa tctccagagt cttttgaagg aactctatga ttcacctagc gcccaacgtt
     1501 acagtgaatc agatggcctg gtctttgacc ctgctaagat tatcagtcgt acaccaaatg
     1561 gagttgcgat tccgcatggc gaccattacc actttattcc ttacagcaag ctttctgcct
     1621 tagaagaaaa gattgccaga atggtgccta tcagtggaac tggttctaca gtttctacaa
     1681 atgcaaaacc taatgaagta gtgtctagtc taggcagtct ttcaagcaat tcttcttctt
     1741 taacgacaag taaggagctc tcttcagcat ctgatggtta tatttttaat ccaaaagata
     1801 tcgttgaaga aacggctaca gcttatattg taagacatgg tgatcatttc cattacattc
     1861 caaaatcaaa tcaaattggg caaccgactc ttccaaacaa tagtctagca acaccttctc
     1921 catctcttcc aatcaatcca ggaacttcac atgagaaaca tgaagaagat ggatacggat
     1981 ttgatgctaa tcgtattatc gctgaagatg aatcaggttt tgtcatgagt cacggagacc
     2041 acaatcatta tttcttcaag aaggacttga cagaagagca aattaaggct gcgcaaaaac
     2101 atttagagga agttaaaact agtcataatg gattagattc tttgtcatct catgaacagg
     2161 attatccaag taatgccaaa gaaatgaaag atttagataa aaaaatcgaa gaaaaaattg
     2221 ctggcattat gaaacaatat ggtgtcaaac gtgaaagtat tgtcgtgaat aaagaaaaaa
     2281 atgcgattat ttatccgcat ggagatcacc atcatgcaga tccgattgat gaacataaac
     2341 cggttggaat tggtcattct cacagtaact atgaactgtt taaacccgaa gaaggagttg
     2401 ctaaaaaaga agggaataaa gtttatactg gagaagaatt aacgaatgtt gttaatttgt
     2461 taaaaaatag tacgtttaat aatcaaaact ttactctagc caatggtcaa aaacgcgttt
     2521 cttttagttt tccgcctgaa ttggagaaaa aattaggtat caatatgcta gtaaaattaa
     2581 taacaccaga tggaaaagta ttggagaaag tatctggtaa agtatttgga gaaggagtag
     2641 ggaatattgc aaactttgaa ttagatcaac cttatttacc aggacaaaca tttaagtata
     2701 ctatcgcttc aaaagattat ccagaagtaa gttatgatgg tacatttaca gttccaacct
     2761 ctttagctta caaaatggcc agtcaaacga ttttctatcc tttccatgca ggggatactt
     2821 atttaagagt gaaccctcaa tttgcagtgc ctaaaggaac tgatgcttta gtcagagtgt
     2881 ttgatgaatt tcatggaaat gcttatttag aaaataacta taaagttggt gaaatcaaat
     2941 taccgattcc gaaattaaac caaggaacaa ccagaacggc cggaaataaa attcctgtaa
     3001 ccttcatggc aaatgcttat ttggacaatc aatcgactta tattgtggaa gtacctatct
     3061 tggaaaaaga aaatcaaact gataaaccaa gtattctacc acaatttaaa aggaataaag
     3121 cacaagaaaa ctcaaaactt gatgaaaagg tagaagaacc aaagactagt gagaaggtag
     3181 aaaaagaaaa actttctgaa actgggaata gtactagtaa ttcaacgtta gaagaagttc
     3241 ctacagtgga tcctgtacaa gaaaaagtag caaaatttgc tgaaagttat gggatgaagc
     3301 tagaaaatgt cttgtttaat atggacggaa caattgaatt atatttacca tcgggagaag
     3361 tcattaaaaa gaatatggca gattttacag gagaagcacc tcaaggaaat ggtgaaaata
     3421 aaccatctga aaatggaaaa gtatctattg gaacagttga gaaccaacca acagaaaata
     3481 aaccagcaga ttctttacca gaggcaccaa acgaaaaacc tgtaaaacca gaaaactcaa
     3541 cggataatgg aatgttgaat ccagaaggga atgtggggag tgaccctatg ttagatccag
     3601 cattagagga agctccagca gtagatcctg tacaagaaaa attagaaaaa tttacagcta
     3661 gttacggatt aggcttagat agtgttatat tcaatatgga tggaacgatt gaattaagat
     3721 tgccaagtgg agaagtgata aaaaagaatt tatctgatct catagcgtaa ggaatagcag
     3781 tagaaaaagt ctgaatcaaa aatgaagttc tctcaaaagt tagaaataaa actctgactt
     3841 tgggagaatt tcattttatt attaatatat aaaatttctt gac
//
LOCUS       DAVMHG010000144         3833 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVMHG010000144 DAVMHG010000000
VERSION     DAVMHG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.146"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..283
                     /locus_tag="VYR08_001861"
     CDS             17..283
                     /locus_tag="VYR08_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV3467342.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            276..644
                     /locus_tag="VYR08_001862"
     CDS             276..644
                     /locus_tag="VYR08_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV3467343.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            764..2032
                     /locus_tag="VYR08_001863"
     CDS             764..2032
                     /locus_tag="VYR08_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV3467344.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2029..3306
                     /gene="tilS"
                     /locus_tag="VYR08_001864"
     CDS             2029..3306
                     /gene="tilS"
                     /locus_tag="VYR08_001864"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV3467345.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3310..>3833
                     /gene="hpt"
                     /locus_tag="VYR08_001865"
     CDS             3310..>3833
                     /gene="hpt"
                     /locus_tag="VYR08_001865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV3467346.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLK"
BASE COUNT         1379 a          534 c          744 g         1176 t
ORIGIN      
        1 aattgaggta ataaggatga gattagataa atatttaaaa gtatcgcgaa ttatcaagcg
       61 tcgtacagtc gcaaaggaag tagcagataa aggtagaatc aaggttaatg gaatcttggc
      121 caaaagttca acggacttga aagttaatga ccaagttgaa attcgctttg gcaataagtt
      181 gctgcttgta aaagtactag agatgaaaga tagtacaaaa aaagaagatg cagcaggaat
      241 gtatgaaatt atcagtgaaa cacgggtaga agaaaatgtc taaaaatatt gtacaattga
      301 ataattcttt tattcaaaat gaataccaac gtcgtcgcta cctgatgaaa gaacgacaaa
      361 aacggaatcg ttttatggga ggggtattga ttttgattat gctattattt atcttgccaa
      421 cttttaattt agcgcagagt tatcagcaat tactccaaag acgtcagcaa ttagcagact
      481 tgcaaactca gtatcaaact ttgagtgatg aaaaggataa ggagacagca tttgctacca
      541 agttgaaaga tgaagattat gctgctaaat atacacgagc gaagtactat tattctaagt
      601 cgagggaaaa agtttatacg attcctgact tgcttcaaag gtgataaaat ggaaaattta
      661 ttagacgtaa tagagcaatt tttgagtttg tcagatgaaa agctggaaga attggctgat
      721 aaaaatcaat tattgcgttt acaagaagaa aaggaaagga agaatgcgta aattcttaat
      781 tattttgttg ctaccaagtt ttttgaccat ttcaaaagtc gttagcacag aaaaagaagt
      841 cgtctatact tcgaaagaaa tttattacct ttcacaatct gactttggta tttattttag
      901 agaaaaatta agttctccca tggtttatgg agaggttcct gtttatgcga atgaagattt
      961 agtagtggaa tctgggaaat tgactcccaa aacaagtttt caaataaccg agtggcgctt
     1021 aaataaacaa ggaattccag tatttaagct atcaaatcat caatttatag ctgcggacaa
     1081 acgattttta tatgatcaat cagaggtaac tccaacaata aaaaaagtat ggttagaatc
     1141 tgactttaaa ctgtacaata gtccttatga tttaaaagaa gtgaaatcat ccttatcagc
     1201 ttattcgcaa gtatcaatcg acaagaccat gtttgtagaa ggaagagaat ttctacatat
     1261 tgatcaggct ggatgggtag ctaaagaatc aacttctgaa gaagataatc ggatgagtaa
     1321 agttcaagaa atgttatctg aaaaatatca gaaagattct ttctctattt atgttaagca
     1381 actgactact ggaaaagaag ctggtatcaa tcaagatgaa aagatgtatg cagccagcgt
     1441 tttgaaactc tcttatctct attatacgca agaaaaaata aatgagggtc tttatcagtt
     1501 agatacgact gtaaaatacg tatctgcagt caatgatttt ccaggttctt ataaaccaga
     1561 gggaagtggt agtcttccta aaaaagaaga taataaagaa tattctttaa aggatttaat
     1621 tacgaaagta tcaaaagaat ctgataatgt agctcataat ctattgggat attacatttc
     1681 aaaccaatct gatgccacat tcaaatccaa gatgtctgcc attatgggag atgattggga
     1741 tccaaaagaa aaattgattt cttctaagat ggccgggaag tttatggaag ctatttataa
     1801 tcaaaatgga tttgtgctag agtctttgac taaaacagat tttgatagtc agcgaattgc
     1861 caaaggtgtt tctgttaaag tagctcataa aattggagat gcggatgaat ttaagcatga
     1921 tacgggtgtt gtctatgcag attctccatt tattctttct attttcacta agaattctga
     1981 ttatgatacg atttctaaga tagccaagga tgtttatgag gttctaaaat gagggaacca
     2041 gattttttaa atcattttct caagaaggga tatttcaaaa agcatgctaa ggcggttcta
     2101 gctctttctg gtggattaga ttctatgttt ctatttaagg tattgtctac ttatcaaaaa
     2161 gagttagaga ttgaattgat tctagctcat gtgaatcata agcagagaat tgaatcagat
     2221 tgggaagaaa aggaattaag gaagttggct gctgaagcag agcttcctat ttatatcagc
     2281 aatttttcag gagaattttc agaagcgcgt gcacgaaatt ttcgttatga tttttttcaa
     2341 gaggtcatga aaaagacagg tgcgacagct ttagtcactg cccaccatgc tgatgatcag
     2401 gtggaaacga tttttatgcg cttgattcga ggaactcgct tgcgctatct atcaggaatt
     2461 aaggagaagc aagtagtcgg agagatagaa atcattcgtc ccttcttgca ttttcagaaa
     2521 aaagactttc catcaatttt tcactttgaa gatacatcaa atcaggagaa tcattatttt
     2581 cgaaatcgta ttcgaaattc ttacttacca gaattggaaa aagaaaatcc tcgatttagg
     2641 gatgcaatct taggcattgg caatgaaatt ttagattatg atttggcaat agctgaatta
     2701 tctaacaata ttaatgtgga agatttacag cagttatttt cttactctga gtctacacaa
     2761 agagttttac ttcaaactta tctgaatcgt tttccagatt tgaatcttac aaaagctcag
     2821 tttgctgaag ttcagcagat tttaaaattt aaaagccagt atcgtcatcc gattaaaaat
     2881 ggctatgaat tgataaaaga gtaccaacag tttcagattt gtaaaatcag tccgcaggct
     2941 gatgaaaagg aagatgaact tgtgttacac tatcaaaatc aggtagctta tcaaggatat
     3001 ttattttctt ttggacttcc attagaaggt gaattaattc aacaaatacc tgtttcacgt
     3061 gaaacatcca tacacattcg tcatcgaaaa acaggagatg ttttgattaa aaatgggcat
     3121 agaaaaaaac tcagacgttt atttattgat ttgaaaatcc ctatggaaaa gagaaactct
     3181 gctcttatta ttgagcaatt tggtgaaatt gtctcaattt tgggaattgc gaccaataat
     3241 ttgagtaaaa aaacgaaaaa tgatataatg aacactgtac tttatataga aaaaatagat
     3301 aggtaaaaaa tgttagaaaa cgatattaaa aaagtcctcg tttcacacga tgaaattaca
     3361 gaagcagcta aaaaactagg tgctcaatta actaaagact atgcaggaaa aaatccaatc
     3421 ttagttggga ttttaaaagg atctattcct tttatggctg aattggtcaa acatattgat
     3481 acacatattg aaatggactt catgatggtt tctagctacc atggtggaac agcaagtagt
     3541 ggtgttatca atattaaaca agatgtgact caagatatca aaggaagaca tgttctattt
     3601 gtagaagata tcattgatac aggtcaaact ttgaagaatt tgcgagatat gtttaaagaa
     3661 agagaagcag cttctgttaa aattgcaacc ttgttggata aaccagaagg acgtgttgta
     3721 gaaattgagg cagactatac ttgctttact atcccaaatg agtttgtagt aggttatggt
     3781 ttagactaca aagaaaatta tcgtaatctt ccttatattg gagtattgaa aga
//
LOCUS       DAVMHG010000145         3758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVMHG010000145 DAVMHG010000000
VERSION     DAVMHG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.147"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            60..272
                     /locus_tag="VYR08_001866"
     CDS             60..272
                     /locus_tag="VYR08_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467347.1"
                     /translation="MRKVIFEDYSYLTNQDVEKLLDSKELTRFQKISLKYAFQEHTPT
                     HKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            265..513
                     /locus_tag="VYR08_001867"
     CDS             265..513
                     /locus_tag="VYR08_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467348.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            609..827
                     /locus_tag="VYR08_001868"
     CDS             609..827
                     /locus_tag="VYR08_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467349.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(1195..3519)
                     /gene="pflB"
                     /locus_tag="VYR08_001869"
     CDS             complement(1195..3519)
                     /gene="pflB"
                     /locus_tag="VYR08_001869"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV3467350.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLNWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1141 a          695 c          732 g         1190 t
ORIGIN      
        1 attatatgat acaggcatcc aaacaatcac aattcaacgc aagccattgg tttcgctatt
       61 tgcgaaaagt tatttttgaa gactattctt atttaacaaa ccaagatgta gaaaagttgc
      121 tagactccaa agaactaacc cgttttcaaa aaattagctt gaagtatgcc tttcaagagc
      181 atactccaac tcataaatat gtgatttcat taaataaacc tgctaagtta accaatgttc
      241 aaaaattgat ggagaaatac aaacatggat aaaatgaaac cggtcttcca agccctaaat
      301 aaggaattaa ttcaggaaaa tctgacttta acaattatct gtgtcgatgg ttatgtctta
      361 gaatatcatg gtttacgtgc cacacaagat gttgatgctt tttatgacca aaatcagaaa
      421 atcaatgaaa ttattgctcg tgtaggaaaa cagtttaatc ttaacatcca cgaagagtta
      481 tggctcaaca atcatgttgc aaagcaaata tgaacaaaca acctccctta agtttatgcg
      541 aatccttgta ctcatttgag aatctgaccg ttttagtagt ccctattgag tatgtgttag
      601 gaatgaagat gatgagtatc agagagcagg atttgcaaga tattggtgca attattaaat
      661 acaaaaactt tcattcacca ttcgatacct ttaaatattt aaaagacatg ggatttgata
      721 ctattgacct ctctgttcta ttagaaggtt ttagttatgc ttatggtatg gattggttag
      781 aaaaattttt taaagaaaat caagataaac ttcgcgaatt ttattaacag tatcaaacaa
      841 gatgattgtc aattagccag tcgtcttgtt ttttatgatg aaatgaaata aaatctgaac
      901 aaatcgctta gagaattcca gtcaatttct agcgctgttt gagacgtttc agtggactat
      961 agtaaaatga aataagaata ggacaaatcg atcaggacag tcaaatcgat ttctaagaat
     1021 gttttagaaa taaaggtgta ctattctagt ttcaatctat tatatttcag attcggtcca
     1081 ctattccttc ccaaaaacat tgcatattgc gtcaaaggaa ttgctcacac tatcccaagc
     1141 acaaaaccct cagtcgattg accgagggtt cctttttatt attcaagaac ttgattagct
     1201 caatgcatcc aaggcgtcat ccattgaaag aacttcgtgg aagacacgtt gtgtcaattc
     1261 agttttttgt tctggagtga ggtatttagt gtttacacag tatccagaga tacgtacgat
     1321 aacgtcttcg cctgacatga tcttttcgta aacatcgttc aagtccataa cgttcaagtt
     1381 aacgtgttgt ccaccgtttt cgaagtaacc atcaaggatt gttaccaagt tatcaacttg
     1441 ttcatcacga gtcttaccaa gagcgcgagg tgatacttgt gtagtcaatg agataccgtc
     1501 agctgcataa ctaaagtcaa ggctagaaag tgagttcaag ttttgcaacc aaccaccttt
     1561 agctttgtta gatgggttag cacctggtga gaagaattca agtttagaca agttcacaga
     1621 accatcttcg ttgaggtata cacctttgtg aactggtgag ttaccagttt gtttagagta
     1681 agcaacgtta gatgtgattg tcaaaagtga tactgtagct tctgcgtctt tgtatagttt
     1741 gtggctacgt agacgagttg tgtaagcttc gatcaaccat tctgccaatt cgtttgaacg
     1801 tgggtcatct tcaccccagc gtgggtagtc accgattgtt tcgtaatcgt agatgtagcc
     1861 atcttcgtca cggattggtt taactgtagc gtatttgata gctgacaatg tatcaacagt
     1921 gttagcaaat ccacagatac cgaatcccat gttggcacgt tgtttagttg gcaagaaggc
     1981 catttgaaca gcttcgtagt tgtacctatc agtcatgtag tggatgatgt tcaaggcatc
     2041 tacgtaagtg tcagtcaacc agttaagaga tttttcaaag ttcgctttaa ctgattcgaa
     2101 ttcaagaact tcgtcacgga ttggttcgat atcaaatact ttgtagtctt tgtgaacatc
     2161 gtcgtaacca ccattcaaac cagtaagaag ggctttaaga acgtttacac gagcaccgaa
     2221 gtactggatg ttgtggcgtt gttcttcatt ttctggatca agtggagaca cacagcatga
     2281 gatacagctc atttcaccat atccgtcttt agccattgtt gttacacctt cgtattggat
     2341 agaagagtgt ttgtggctca tgtgcataca gtagcgacgg aagttgtatg gcaatttgtc
     2401 agtccaaaga actgtcaagt ttggttctgg tgagttaccg atgttgtcaa gagtgttcaa
     2461 gaaacggtag tccatcttag taacacggtg acgaccgtcg ttacccatac cagccataga
     2521 agttgtgata aaggttgggt cacctgagta caattggtca taagcttttg tacgagcaaa
     2581 tttaactgta cgaagtttca taacgaaatc atcaacgaat tcttggattt ctgattcagt
     2641 aaatgtacca cgagcaaggt cacgttctgc aaagatgtcc aatacgattg gtacacgacc
     2701 tagagatgta gcagcaccgt taatcacacg gcagacagcc atgaaagcaa tgttaaccca
     2761 ttggattgct tctttcacgt tcatcgctgg tttgcgaaca tcaaccccgt aaaggtcacc
     2821 caggcgaaca acttgttgca atgcttggta ttgaaggttt acttcttcac gaagacggat
     2881 tgtttcttca tcgatttctt tgattgcatt ccagtcgttt actttttctt gcatcaagta
     2941 gtctgcaccg taaagagcaa gacgtgcgta aacaccgatg atacgtccgc gtgagtatgc
     3001 atctggaaga ccagttacag tgtgtgcatg acgagcgcga cgaatatttg aagtgtaggc
     3061 acggaaaata ccgtcgttaa ctgttgttac atatttagtg aagatttcgt gaacagctgg
     3121 gtctggttcg tatccatttt cttttaaagt agtttcagcc atacggatac caccttttgg
     3181 catgaagttc aatttgaaga gttcatcatt ttggatacca aagataactt cattttcttt
     3241 gtcgataaat ccagcaggga tatcagcgat agatgttgga cgagtgtcca ttgggaaacg
     3301 agtttcttcg tagtgtgctt tagtttcttc tacaattttc ttgatgtgaa gtgaacgctc
     3361 tgttggtcct gcaaggaagc tttcgtctcc atcataaggt gtgtagttag cttgtacaaa
     3421 gcgtgataca cttgcttttt ctttccaatc tacgcctttg aagccttccc aagctttgtc
     3481 aaaaatatct tgtgcttcaa caactgtctt aacaaccata ttaatatcct cttttttcta
     3541 gtaacagtca tctgttacat tcataagaca agtataccat aaagtaaccg atttcaacaa
     3601 gtgcaaaatc cctattttta cactttcttt tctaaaacag tctatatttt atttcaaact
     3661 gtattatatt tttgaaaaaa ataaagtcct tttttctttt tttcagaaaa aagggtataa
     3721 taaaagaaaa taagcagtaa cactcaatgg aaatcgaa
//
LOCUS       DAVMHG010000146         3683 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVMHG010000146 DAVMHG010000000
VERSION     DAVMHG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.148"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..331
                     /locus_tag="VYR08_001870"
     CDS             104..331
                     /locus_tag="VYR08_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467351.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            619..3516
                     /gene="nanA"
                     /locus_tag="VYR08_001871"
     CDS             619..3516
                     /gene="nanA"
                     /locus_tag="VYR08_001871"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV3467352.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNDQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1256 a          619 c          837 g          971 t
ORIGIN      
        1 ctatctttat gtatatttaa agatagattg ctgtaaaaat agtagtagct atgcgaaata
       61 acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat ttaaggcatt
      121 caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt ttttcgacag
      181 ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc tggataagat
      241 tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg cttatgaaaa
      301 gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtgtaaa tagcataagc
      361 tgatgtccat catttgctta taaagagata ttttagttta attgcagcgg tgtcctggta
      421 gataaactag attggcagga gtctgattgg agaaaggaga ggggaaattt ggcaccaatt
      481 tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa agaaagttaa
      541 taaaagacaa actaagtgca ttttctggaa taaatgtctt atttcagaaa tcgggatata
      601 gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg ttatagcatt
      661 aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt tggaacgtct
      721 cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac tcaactttcg
      781 ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga gactgaactt
      841 tctggcaata agcaagaaca agaaaggaaa gataagcaag aagaaaaaat tccaagagat
      901 tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga tgttgaaacc
      961 aatgcttcaa atgatcagag agttgattta tcaagtgaac tagataaact aaagaaactt
     1021 gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggccccagc attctataat
     1081 ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc agtttacaat
     1141 aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacaa taattacaac
     1201 gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac agttgaaaaa
     1261 ccgacagcag aattgccaaa tggtcgagtt cgcctctacg taaacggggt attatctcga
     1321 acaagtctga aatctggcaa tttcattaaa gatatgccag atgtaacgca tgtgcaaatc
     1381 ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat tcggaatctc
     1441 actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca actttttaaa
     1501 cgctcagatt tagaaaaaaa actacctgaa ggagcggctt taacagagaa aacggacata
     1561 ttcgaaagcg ggcgtaacgg taaaccaaat aaagatggaa tcaagagtta tcgtattcca
     1621 gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg ccgtctccat
     1681 tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa tggtaaaact
     1741 tggggtgacc gagtaaccat taccaactta cgtgacaatc caaaagcttc tgacccatcg
     1801 atcggttcac cagtgaatat cgatatggtg ttggttcaag atcctgaaac caaacgaatc
     1861 ttttctatct atgacatgtt cccagaaggg aagggaatct ttggaatgtc ttcacaaaaa
     1921 gaagaagcct acaaaaaaat cgatggaaaa acctatcaaa tcctctatcg tgaaggagaa
     1981 aagggagctt ataccattcg agaaaatggt actgtctata caccagatgg taaggcgaca
     2041 gactatcgcg ttgttgtaga tcctgttaaa ccagcctata gcgacaaggg tgatctatac
     2101 aagggtgacc aattactagg aaatatctac ttcacaacaa acaaaacttc tccatttaga
     2161 attgccaagg atagctatct atggatgtcc tacagtgatg acgacgggaa gacatggtca
     2221 gcgcctcaag atattactcc gatggtcaaa gccgattgga tgaaattctt gggtgtaggt
     2281 cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt gataccggtt
     2341 tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt catctattca
     2401 gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg tcaggtagac
     2461 ggtcaaaaga tccactcttc tacgatgaac aatgaacgtg cgcaaaatac agaatcaacg
     2521 gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac tggagatctt
     2581 caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa acgttatcca
     2641 caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcacga aggaaaagaa
     2701 tacatcatcc tcagtaatgc aggtggaccg aaacgtgaaa atgggatggt ccacttggca
     2761 cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca aaaaggagag
     2821 tttgcctata attcgctcca agaattagga aatggggagt atggcatctt gtatgaacat
     2881 actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg ggaatttttg
     2941 agcaaagatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga gatgggcaaa
     3001 ggagagatgg gcaaaggagt tattggcttg gagttcgact cagaagtatt ggtcaacaag
     3061 gctccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca gtatgatagc
     3121 aagaccttgt tgtttgcagt agataaggaa gatatcggac aggaaattat tggtatagct
     3181 aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc cagagttcct
     3241 ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac agggggagtt
     3301 aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga ttcgcttgta
     3361 actcttacta caaaagaaga ttatacttac aaagctcctc ttgctcagca ggcacttcct
     3421 gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc tttcttcctt
     3481 ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa acatttgatt
     3541 ttgtaaaaat agaaggagat agcaggtttt caagcctgct atcttttttt gatgacattc
     3601 aagctgatac gaaatcataa gaggtctgaa actactttca gagtagtctg ttctataaaa
     3661 tatagtagat tgaaataaga tgt
//
LOCUS       DAVMHG010000147         3654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVMHG010000147 DAVMHG010000000
VERSION     DAVMHG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.149"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..750
                     /locus_tag="VYR08_001872"
     CDS             70..750
                     /locus_tag="VYR08_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3467353.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            710..1090
                     /locus_tag="VYR08_001873"
     CDS             710..1090
                     /locus_tag="VYR08_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV3467354.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            1100..1387
                     /locus_tag="VYR08_001874"
     CDS             1100..1387
                     /locus_tag="VYR08_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV3467355.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            1388..2145
                     /locus_tag="VYR08_001875"
                     /pseudo
     CDS             1388..2145
                     /locus_tag="VYR08_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            2157..3022
                     /locus_tag="VYR08_001876"
                     /pseudo
     CDS             2157..3022
                     /locus_tag="VYR08_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3102..3647)
                     /locus_tag="VYR08_001877"
     CDS             complement(3102..3647)
                     /locus_tag="VYR08_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467356.1"
                     /translation="MTGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIPANL
                     GALIEEPGFINYYSGFKNLQYLASIRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGM
                     RKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHSEED
                     IRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1053 a          815 c          681 g         1105 t
ORIGIN      
        1 tagatattat ccaaagaaga ttttaagtgc tacaataact gtattatttc tagatgggag
       61 gttctatttt tggattggtc cattgttgaa caatatctac cactatatca aaaggcattc
      121 tttctgacct tgcatattgc agtttgggga attttgggat cctttctgct cggtttaatc
      181 gttagtatca tccgacatta tcgaatccct gttttggcgc aagtagcgac agcctacatt
      241 gaattgtcac gtaatacgcc ccttttgatt caactcttct ttctctactt cggtcttccc
      301 cgaatcggga ttgtcctatc ttcagaagtc tgtgcaacgc ttgggcttgt ctttttagga
      361 ggctcctata tggcagaatc tttccgaagt gggctggaag ccatcagtca aacccagcag
      421 gagattggcc tcgctattgg tctgacacct ctacaggtct ttcgctatgt ggttcttccg
      481 caagcaacag cggtggcact cccctccttt agtgccaatg tcattttcct tatcaaggaa
      541 acctctgttt tctcagcagt ggctttggcc gacctcatgt acgtcgccaa ggatttgatt
      601 ggtctctact atgagacaga cattgcgcta gctatgttgg tagttgctta tctgatcatg
      661 ctgctaccca tctcactggt ctttagctgg atagaaagaa ggacccgcca tgcaggattc
      721 gggaatccaa gtactctttc aaggaaataa tctcctgaga atcttacagg ggttgggcgt
      781 tacgattggg atatccatcc tgtctgtcct cttatccatg atgttcggaa cagtcatggg
      841 aatcatcatg acctcccatt ctagaatcat acgattttta acacgattgt atctggaatt
      901 tatccgtatc atgccccagc tggtgctact cttcatcgtt tactttggct tggctcgaaa
      961 ctttaatatc aatatctcag gtgagacttc agctattatc atttttaccc tctggggaac
     1021 agctgaaatg ggagacttgg tacgtggagc tatcacttct ctccctaaac atcagtttga
     1081 aagtggatag gcactcggct tgactaatgt tcaactttac taccacatca tcatcccaca
     1141 agtcttaaga agactgctac cgcaggctat caatcttgtc actcggatga ttaaaaccac
     1201 ttcattagtt gttttgattg gggttgtgga agtgaccaaa gttggacaac aaatcatcga
     1261 tagcaatcgc ctgaccatcc caactgcttc attttggatt tatggaacca ttctagtctt
     1321 atatttcgca gtttgctacc ctatttccaa actatccact cacttagaaa aacattggag
     1381 aaactaaatg tctgaaacta tcttagaaat caaggaacta aaaaaatcct tcggagacaa
     1441 tcccatcctc caaggacttt ctctagaaat caaaaaaggg aagttgttgt catcctaggg
     1501 ccatctggtt gtgggaaaag taccctcctt cgttgcctca acggcttaga aagtattcaa
     1561 ggtggagata tttttctgga tggtcagtct atcgttgaaa ataaaaaaga ttttcaccta
     1621 gttcgccaaa agattggcat ggtctttcaa agttatgaac tctttcccca tctggatgtc
     1681 ttacaaaacc tcatcctagg ccctatcaaa gctcaaggaa gggacaagaa agaagtaacg
     1741 gaagaagctt tgcaattact agagcgtgtc ggtttgctgg ataaacaaca tagctttgcc
     1801 cgtcaattat ctggtggaca gaagcaacgt gttgcaattg tccgtgccct cctaatgcat
     1861 ccagaaatca tcctttttga cgaggtgact gcttcgctgg atccagaaat ggtgcgtgag
     1921 gtgctggaac ttatcaatga tttggcccaa gaaggccgta ccatgatttt agtaacccac
     1981 gaaatgcagt ttgcccaagc cattgctgac cagattatct tcctcgacca agggaaaatc
     2041 gctgaagaag gaacagctca agccttcttt accaatccgc aaaccaaacg agcccaggaa
     2101 tttttaaacg tctttgactt tagccaattc ggctcatatc tataaaggag attcttatga
     2161 aactattcaa accactctta actgttttag cacttgcctt tgcccttatc tttatcactg
     2221 cttgtagctc aggtggaaac gctggttcat cctctggaaa aacaactgcc aaagctcgca
     2281 ctatcgatga aatcaaaaaa agcggtgaac tgcgaatcgc cgtgtttgga gataaaaaac
     2341 cgtttggcta cgttgacaat gatggttctt accaaggcta cgctacgata ttgaactagg
     2401 gaaccaacta gctcaagacc ttggtgtcaa ggttaaatac atttcagtcg atgctgacaa
     2461 ccgtgcggaa tacttgattt caaacaaggt agatattact cttgctaact ttacagtaac
     2521 tgacgaacgt aagaaacaag ttgattttgc ccttccatat atgaaagttt ctctgggtgt
     2581 cgtatcacct aagactggtc tcattacaga cgtcaaacaa cttgaaggta aaaccttaat
     2641 tgtcacaaaa ggaacgactg ctgagactta ttttgaaaag aatcatccag aaatcaaact
     2701 ccaaaaatac gaccaataca gtgactctta ccaagctctt cttgacggac gtggagatgc
     2761 cttttcaact gacaatacgg aagttctagc ttgggcactt gaaaataaag gatttgaagt
     2821 aggaattact tccctcggtg atcccgatac cattgcggca gcagttcaaa aaggcaacca
     2881 agaattgcta gacttcatca ataaagatat tgaaaaatta ggcaaggaaa acttcttcca
     2941 caaggcctat gaaaaaacac ttcacccaac ctacggtgac gctgctaaag cagatgacct
     3001 ggttgttgaa ggtggccatt aactcttaaa agggactgga ttttaagctc caatcccttt
     3061 ttaagatttt acctataaca tcctgagtct atctaagatg ttcaatctga acacagtgta
     3121 catactttat cttctattgc atatacttta tcacataaga tacgaatatc ctcttcacta
     3181 tgactagcaa tcaaaattgt tgtccctttt tcactagaga gctttctaaa caatgttctc
     3241 atattttcta cacttgattt atccaaggca ttcataggtt catctagtaa aagaatagag
     3301 ggattctcca taattgcttg agcaatccct agctttttcc tcatacctag cgaataagtt
     3361 ttaactttct ggtctttttg ctcatataga ccaactattt tcagtgtatc attgatttcc
     3421 tgattaccaa ctactcctcg tatgcttgcc aaatattgta aattcttaaa gccactataa
     3481 taatttataa aaccaggttc ttcaatcaaa gctcccaaat tagctggaat ttttctctca
     3541 ggaacaatat tttccccatt gattaacact tctccataag acggactata taaaccagct
     3601 attaatttaa acaatacact tttccctgag ccattcgcac cagtaattcc tata
//
LOCUS       DAVMHG010000148         3607 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVMHG010000148 DAVMHG010000000
VERSION     DAVMHG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.150"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>3607
                     /locus_tag="VYR08_001878"
     CDS             <1..>3607
                     /locus_tag="VYR08_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV3467357.1"
                     /translation="TPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLK
                     SINAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLS
                     DKIPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAP
                     NAQIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAI
                     RALRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAK
                     NQTVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIG
                     LDLRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEAD
                     EGTKSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKP
                     NVGDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLN
                     VINGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKI
                     ALQNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNS
                     YGSISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSP
                     DGKQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHF
                     ESVEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDD
                     DGKPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPS
                     SSGSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLK
                     VISREHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDP
                     ATKIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIK
                     LITKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEV
                     TYAGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQAD
                     EKGMISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENE"
BASE COUNT         1374 a          531 c          732 g          970 t
ORIGIN      
        1 aactccagat aacttggaca aaattaaaca aatagaaggt attacatcgg ttgaaagggc
       61 acaaaaagtc caacccatga tgaatcatgc cagaaaggaa attggagttg aggaagctat
      121 tgattaccta aagtctatca atgctccgtt tgggaaaaat tttgatggta gaggtatggt
      181 catttcaaat atcgatactg gaacagatta tagacataag gctatgagaa tcgatgatga
      241 tgccaaagcc tcaatgagat ttaaaaaaga agacttaaaa ggaactgata aaaattattg
      301 gttgagtgat aaaatccctc atgcgttcaa ttattataat ggtggcaaaa tcactgtaga
      361 aaaatatgat gatggaaggg attattttga cccacatggg atgcatattg cagggattct
      421 tgctggaaat gatactgaac aagatatcaa aaactttaac ggcatagatg gaattgcacc
      481 taatgcacaa attttctctt acaaaatgta ttctgacgca ggatctgggt ttgcgggtga
      541 tgaaacaatg tttcatgcta ttgaagattc tatcaaacac aacgttgatg ttgtttcggt
      601 atcatctggt tttacaggaa caggtcttgt aggtgagaaa tattggcaag ctattcgggc
      661 attaagaaaa gcaggcattc caatggttgt cgctacgggt aactatgcga cttctgcttc
      721 aagttcttca tgggatttag tagcaaataa tcatctgaaa atgaccgaca ctggaaatgt
      781 aacacgaact gcagcacatg aggatgcgat agcggtcgct tctgctaaaa atcaaacagt
      841 tgagtttgat aaagttaaca taggtggaga aagttttaaa tacagaaata taggggcctt
      901 tttcgataag aataaaatca caacaaatga agatggaaca aaagctccta gtaaattaaa
      961 atttgtatat ataggcaagg ggcaagacca agatttgata ggtttggatc ttaggggcaa
     1021 aattgcagta atggatagaa tttatacaaa ggatttaaaa aatgctttta aaaaagctat
     1081 ggataagggt gcacgcgcca ttatggttgt aaatactgta aattactaca atagagataa
     1141 ttggacagag cttccagcta tgggatatga agcggatgaa ggtactaaaa gtcaagtgtt
     1201 ttcaatttca ggagatgatg gtgtaaagct atggaacatg attaatccta ataaaaaaac
     1261 tgaagtcaaa agaaataata aagaagattt taaagataaa ttggagcaat actatccaat
     1321 tgatatggaa agttttaatt ccaacaaacc gaatgtaggt gacgaaaaag agattgactt
     1381 taagtttgca cctgacacag acaaagaact ctataaagaa gatatcatcg ttccagcagg
     1441 atctacatct tgggggccaa gaatagattt acttttaaaa cccgatgttt cagcacctgg
     1501 taaaaatatt aaatccacgc ttaatgttat taatggcaaa tcaacttatg gctatatgtc
     1561 aggaactagt atggcgactc caatcgtggc agcttctact gttttgatta gaccgaaatt
     1621 aaaggaaatg cttgaaagac ctgtattgaa aaatcttaag ggagatgaca aaatagatct
     1681 tacaagtctt acaaaaattg ccctacaaaa tactgcgcga cctatgatgg atgcaacttc
     1741 ttggaaagaa aaaagtcaat actttgcatc acctagacaa cagggagcag gcctaattaa
     1801 tgtggccaat gctttgagaa atgaagttgt agcaactttc aaaaacacgg attctaaagg
     1861 tttggtaaac tcatatggtt ccatttctct taaagaaata aaaggtgata aaaaatactt
     1921 tacaatcaag cttcacaata catcaaacag acctttgact tttaaagttt cagcatcagc
     1981 gataactaca gattctctaa ctgacagatt aaaacttgat gaaacatata aagatgaaaa
     2041 atctccagat ggtaagcaaa ttgttccaga aattcaccca gaaaaagtca aaggagcaaa
     2101 tatcacattt gagcatgata ctttcactat aggcgcaaat tctagctttg atttgaatgc
     2161 ggttataaat gttggagagg ccaaaaacaa aaataaattt gtagaatcat ttattcattt
     2221 tgagtcagtg gaagaaatgg aagctctaaa ctccaacggg aagaaaataa acttccaacc
     2281 ttctttgtcg atgcctctaa tgggatttgc tgggaattgg aaccacgaac caatccttga
     2341 taaatgggct tgggaagaag ggtcaagatc aaaaacactg ggaggttatg atgatgatgg
     2401 taaaccgaaa attccaggaa ccttaaataa gggaattggt ggagaacatg gtatagataa
     2461 atttaatcca gcaggagtta tacaaaatag aaaagataaa aatacaacat ccctggatca
     2521 aaatccagaa ttatttgctt tcaataacca agggatcaac gctccatcat caagtggttc
     2581 taagattgct aacatttatc ctttagattc aaatggaaat cctcaagatg ctcaacttga
     2641 aagaggatta acaccttctc cacttgtatt aagaagtgca gaagaaggat tgatttcaat
     2701 agtaaataca aataaagagg gagaaaatca aagagactta aaagtcattt cgagagaaca
     2761 ctttattaga ggaattttaa attctaaaag caatgatgca aagggaatca aatcatctaa
     2821 actaaaagtt tggggtgact tgaagtggga tggactcatc tataatccta gaggtagaga
     2881 agaaaatgca ccagaaagta aggataatca agatcctgct actaagataa gaggtcaatt
     2941 tgaaccgatt gcagaaggtc aatatttcta taaatttaaa tatagattaa ctaaagatta
     3001 cccatggcag gtttcctata ttcctgtaaa aattgataac accgccccta agattgtttc
     3061 ggttgatttt tcaaatcctg aaaaaattaa gttgattaca aaggatactt atcataaggt
     3121 aaaagatcag tataagaatg aaacgctatt cgcgagagat caaaaagaac atcctgaaaa
     3181 atttgacgag attgcgaacg aagtttggta tgctggcgcc gctcttgtta atgaagatgg
     3241 agaggttgaa aaaaatcttg aagtaactta cgcaggtgag ggtcaaggaa gaaatagaaa
     3301 acttgataaa gacggaaata ccatttatga aattaaaggt gcgggagatt taaggggaaa
     3361 aatcattgaa gtcattgcat tagatggttc tagcaatttc acaaagattc atagaattaa
     3421 atttgctaat caggctgatg aaaaggggat gatttcctat tatctagtag atcctgatca
     3481 agattcatct aaatatcaaa agcttggcga gattgcagaa tctaaattta aaaatttagg
     3541 aaatggaaaa gagggtagtc taaaaaaaga tacaactggg gtagaacatc atcatcaaga
     3601 aaatgaa
//
LOCUS       DAVMHG010000149         3585 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVMHG010000149 DAVMHG010000000
VERSION     DAVMHG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.151"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(242..853)
                     /gene="rpsD"
                     /locus_tag="VYR08_001879"
     CDS             complement(242..853)
                     /gene="rpsD"
                     /locus_tag="VYR08_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV3467358.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1048..2100)
                     /locus_tag="VYR08_001880"
     CDS             complement(1048..2100)
                     /locus_tag="VYR08_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV3467359.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2097..2795)
                     /locus_tag="VYR08_001881"
     CDS             complement(2097..2795)
                     /locus_tag="VYR08_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467360.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2962..>3585)
                     /locus_tag="VYR08_001882"
     CDS             complement(2962..>3585)
                     /locus_tag="VYR08_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV3467361.1"
                     /translation="MLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVA
                     LDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKE
                     TSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMS
                     DTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          987 a          804 c          696 g         1098 t
ORIGIN      
        1 acctgttcgc tcacaataag agagaacttt tttacgaaaa tctattgaat atgccataaa
       61 agattatacc acattgtgta ctatttttgg ttcattttac tatatcactt tggactatat
      121 cggtctagtt atgtctcaac gattatagct ggatactaag agaaatcaaa aaagcactaa
      181 gggagaacgt cccaaagtgc ttaatatcaa ggcctcaaag cctgtcttat tcaataaaat
      241 attacaacat cttgttgtag aattcaacga caagtgcttc gttgatttct gggttgattt
      301 cgtcgcgttc tggcaagcga gtcaatgaac cttccaactt ttcagcgtcg aatgatacga
      361 atgctggacg tccaagagta gcttctactg cttcaaggat tgctggaact ttcaatgatt
      421 tttcacgaac tgagatcact tgacctggag ttacgcggta tgatgggata tcaacgcgtt
      481 tcccgtcaac aaggatgtga ccgtggttta cgaattgacg agcttgacga cgagtagtcg
      541 cgagaccaag acggtaaaca acgttatcca aacgacgctc caaaagaagc ataaagttga
      601 aacctaggat tccgcctttg atttttgtag cttgtacgaa caagttacgg aattgttttt
      661 cacctacacc gtaagtgaaa cgaagttttt gtttttcagc caattgcaaa ccgtattctg
      721 acaatttaga acggttgttt gggccgtgtt gtcctggtac gtagttacga cgtgccaatt
      781 ctttacctgt acctgtaagt gaaaggccaa ggcgacgagc ttgtttccaa gatggtcctg
      841 tataacgtga catatgaatg tcctcctgat ataaataata tttcggtgga aatagtcact
      901 tagaaagccc tgattcgtgc agatgccctt cgcctaaaca gccaaggtta cttgtcataa
      961 gacacctgtt gacgagcttc atgctttcct gctgatattt cacacaaaat ccattttacc
     1021 atgaatagct ggatttgtaa aggggtttta ggctttattt tcactaccag agaggttgag
     1081 aacgagagta aaggtacttc ctagaccgta ctggctgctg actgtgattt ccccacccaa
     1141 ttgatgggcc aattcacgcg caatcgcaag tcctaatcca tgaccacctg tcttcatgtt
     1201 acgcgaagtt tcgacacgat aaaggcgttt gaaaatattt tccaaatcct ctggggcaat
     1261 accctgccct tcatcggtca cactgattga aagctggtcc ttctccagct tagccaccac
     1321 ttccagcttg gttcctggag cagaatattt aaaagcgtta tcgaccagat tcaccaagat
     1381 acgagaaagc ttagcataat ctccctcaat ccgggcagac tctgggatta cctgcaagtg
     1441 gacatctctt ctctcctgct caatcaaaaa ctgaaattca ctcatgcact caattaagag
     1501 cttgtccaga aaaatactgt ctttactggt agtttccacc tgatttctag ctgtgtttag
     1561 ggtcaaaaaa ttcaactcct caaccagttt attgagcctc tccgtctggc gtccaatggt
     1621 tgctagataa tgagcttgct ccgactcctt gataatccca tccaaaatcc cttctaccgt
     1681 cgcttggatc gaagtgatag gagtcttaat atcatgcgac aactgagcaa tcatcaagcc
     1741 cttttctcgt tcgctttctt ccaaggaatc aaagcttacc tgcaaatcat gggacatctc
     1801 attaaaagtt tgccctaatt gctgaaattc tacaggacct tgaacctcca aatttgaagg
     1861 aaaatccttg gccgctaccc gcttggcatg ctccttgagt ttgcccaacg acgtaaagac
     1921 tggcaatagg agaaagagac taatcccagc accgacaagg ctggcaacga tggtcatccc
     1981 aagcaaaaag taaatctcgc ctttcgcaat cagcattttt tgaacagccc aaaaaacaac
     2041 caaaatggtt aagagggttg aaataatata tccaaccaaa atataacttt ttagtttcat
     2101 gtttgtcctc tcggtttctc tatcttatat cccaaccccc aaactgtctt aatagtgggc
     2161 gtttggtcac tactatattt tgccagctcc tgtcgaagag catggatatg cacattcaag
     2221 gtattggtgt catccacgta gtcttctttc cagatctttt catagaggtc tgtcttggag
     2281 aaaactcgct ctggattgct agctaaaatc cacagcaatt caaatgattt aacagttaaa
     2341 tccagcattt cttctcctat ttgaacttca tgactactat gattcatttt tagattgcca
     2401 agggaaatca gctctgtttc gcccccacga tgaaggcggc gcaaaatatt gtggacacgc
     2461 aaaactagct cacgtgggct aaaaggctta gcaataaaat catctgctcc caagctcagg
     2521 ccgtaaatct tgtcctgttc actggtctta gcagtaataa ataggaaagg ctgctctggt
     2581 gataagtatt gaacctcact gattaaatca taaccatcca tccgaggcat catgacatct
     2641 gtgataatca aatcaatcgg ttttttcttg aacagctcta gcgcttccag tccatcatgg
     2701 gctaccaaga cctgataacc tgcctgaatt aagtatctct gatgaatatc tgtgatttct
     2761 acctcgtcgt caacgagtaa aattgtcttt cccatctgtc tctcctttga taaaaacagt
     2821 gctatactgc tttaagtata acactatttt tattgtttta cgatgatttt aatctgctat
     2881 tgaaagaaag tcctgattgc tgattgagtt gttaaactaa tcttagtcag cccactatcc
     2941 atcatttatt tccttagctg attagttttc ttcttttttg tttttcaaac ctagaccacc
     3001 gagtaaacct gcaagcgcaa gcccaaggaa accaatactt gccattgatg tttgagtctc
     3061 accagtattt ggtagcatag ctttatcctc tgacatcatc gtatcagaca tcttgttagc
     3121 agaagcagcc atgttttcac ctgccatcgt gttggtagaa cttgtcatgg tgtcagcagg
     3181 catgctatct gtagtacctg tagcatgatt gtgattcatc ggagtcacgc cagaaccaga
     3241 gttagaaggt gataatgaac catttgctgt gtctgatgtt tctttaacat ttatcttaat
     3301 agtgactttt ttagttgcta cgatgttgtc caagtctggt ttaccatctt tgttaccata
     3361 gacattgact gtagcgctgt aagtttgagt accatttgct cggaactggt caatgagcgc
     3421 ttgtttttct ttgccagcta catttccgtc caaggctact tgatagaagt attgaccttt
     3481 ggtcttcacg ttttcaccta gtggagatag ggctgggttt ttagcgtcac cgttatctga
     3541 ccatggtgcc ttgtcagatg ccttgagcaa gagacgagtc aacat
//
LOCUS       DAVMHG010000150         3516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVMHG010000150 DAVMHG010000000
VERSION     DAVMHG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.152"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..1436
                     /locus_tag="VYR08_001883"
     CDS             27..1436
                     /locus_tag="VYR08_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3467362.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1454..2749
                     /locus_tag="VYR08_001884"
     CDS             1454..2749
                     /locus_tag="VYR08_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV3467363.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2754..3062
                     /locus_tag="VYR08_001885"
     CDS             2754..3062
                     /locus_tag="VYR08_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV3467364.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3059..3367
                     /locus_tag="VYR08_001886"
     CDS             3059..3367
                     /locus_tag="VYR08_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV3467365.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1183 a          600 c          685 g         1048 t
ORIGIN      
        1 atagaataat agaaaggagg ttgagcatga aaaaaaagtt agtatttcct aatctgtttt
       61 ggtggggagc agcttctagc ggacctcaga cagaaggtca atatggaaaa gtacatgaaa
      121 atgtgatgga ctactggttc aaaacgcatc cagaagattt tttcgataat gtcggacctc
      181 ttgtagccag taactttttt catacttaca ccgaagattt ccacttgatg aaggaaattg
      241 gagttaattc tttccgcact tccatccaat ggagtcgact catcaagaat ttagagacag
      301 gtgagcctga tccaaaaggt attgctttct acaatgccat cattgaagaa gctaaaaaga
      361 accagatgga tcttgtgatg aatttacatc attttgattt accagtggaa cttcttcaaa
      421 aatacggtgg ttgggaaagc aaacatgtag tggagttatt cgtgaagttt gccaagactg
      481 ctttcacatg ctttggagat aaggttcatt actggacaac tttcaatgag ccaatggtca
      541 ttccagaagc aggatactta tatgctttcc attatccaaa tctaaaagga aagggaaaag
      601 aggccgtaca agtcatctat aatctaaacc ttgctagtgc aaaagtgatt caactatatc
      661 gctcattaga acttgatgga aagattggga ttattttaaa cttgacacct gcttatccaa
      721 gaagtaattc tccagaagac ttagaagcaa gtcgatttac agatgacttc tttaacaaag
      781 tcttcttgaa tccagctgtt aaaggaactt tcccagaaag attggtaaaa cagctagaga
      841 gagatggcgt gttatggagt cataccgaaa aagagcttca actgatgaaa tcaaatacgg
      901 ttgattttct tggagtaaac tactaccatc caaaacgtgt tcaagcacaa gcaaatcctg
      961 aggaatatca gacgccctgg atgccagacc aatacttcaa agagtatgaa tggccggagc
     1021 gtcgcatgaa tccatatcgt ggttgggaaa tttttccgaa atccatttat gatattgcta
     1081 tgattgtgaa ggaagaatat ggtaatatcc catggtttat cagtgaaaac ggaatgggtg
     1141 ttgaaaacga agcacggttt atcgatgaaa atggagttat cgatgacgtg tatcgtattg
     1201 aattttatga agaacattta agatggctac ataaagccat tgaagaggga agtcactgtt
     1261 ttggatacca cgcttggacc gcatttgatt gctggtcttg gaataatgca tataagaatc
     1321 gttacggatt tatctccgtt gatttagaaa cgcaaaagag aaccatcaag agctcaggaa
     1381 gatggtatcg caaagtaagt gacaataacg gttttgaagt agaaattgag gagtaaagag
     1441 aggagatatt catatgaaaa actcaaaatt tatagaccaa tttgccacct ttgctggtaa
     1501 actagggaac caaattcatt taaaaaccct aagagatgca ttcgtaacag taatgccatt
     1561 atatattttg gcaggtttaa tcgttctttt gaacaacacg gtatttaagt ggattttcca
     1621 aggggataca ttaacaagat tccaatattg gggaataaca attgcaaacg gtactttaag
     1681 tatttcaggt atgattattg ctgtaatggt tggttatttc ttagctaaaa acagagattt
     1741 cgaaaacccg ttagcagcat caatgctatc attagtttct ttaattgtga tgatgccaaa
     1801 tacagtttct gtagttcctg acggagcaaa agatgctgta aacatttcag gtgttctttc
     1861 attcaacaac acaggtacag gcgcaatgtt cgccggggtt atcgtagcga ttattgcaac
     1921 agaattattc attgaattat caaatgttaa agctttacaa atgaaccttg gtgaaaatat
     1981 tccaccagct gttagtagat catttagcgt attacttcca gtcatgaccg tcatctcctt
     2041 atttggggtt gtttcagcat tattattcaa tataactgga atgaacttaa tctcaatcat
     2101 tacaatcttt attcaagaac caattcgtca tattggtaca agcttaatcg gggtcattat
     2161 tatttactct ttaggaaata tgttatggct atttggtatt caccaagcag ttatttacag
     2221 tgccatccta gaaccattac tattaattaa cattactgaa aacatcactg cagcaaataa
     2281 tggacaagcc attccacaca tcatcaacct atcacaaata caaacattcg ctttaatggg
     2341 tggtagtgga tctacattat gtttattaat agcaacattc ttagtgagtc gcaatgctgt
     2401 ctctaaaaac gtggctaaat tatcttttgg acctggtatc ttcaatatca atgaaccagt
     2461 attattcggt tacccaatcg tttataacat ttcattagct attccattta tcacagttcc
     2521 agtccttggt attttaatca gctacttagc aacagttaca ggattcatga gtcctgcatt
     2581 tatacaagtt ccttggacta caccagtatt cttaaatgca tggttagcaa cagcagggga
     2641 cgtgagagca gttctagttc aattcatcat ctttgcactt ggagttcttc tatacattcc
     2701 atttatcaaa gttaatgaca aagttgttga acaagaaatg gaaggttaag attatgaaac
     2761 aaattttatt agtatgtaat gcgggaatgt cgacaagtat gcttgttaaa aaaatgcaac
     2821 aaagtgcgac tgaacgtggg attgaaatct ctatccaagc aaaatcaatg acagaagcta
     2881 aaaagaatat ccatgaagca gatgtcattc taattgggcc acaaattcgt tatgagttac
     2941 ttgctgtgaa agaaattgca ggaaatatcc ctgtagatac cattgatatg cgggattacg
     3001 gaatgatgaa tggagcaaaa gttctggaac aagcacttgc atggatagga gaaataagat
     3061 gacagaagaa atggaatata tctgttttca gctcattgcc aatagtggag cagccaaatc
     3121 atcctttatt gaagccatcc aactagctaa ggcaggaaat ttaaaagaag cgaaaattaa
     3181 agttgaagaa gcggaagact ccttagtaga agcgcacaag attcactcta atctcataca
     3241 aaaagaagct actggagaaa aaataggatt ctccttgcta tttatgcatg cagaagacca
     3301 aatggcttca acagagatta ttcagttgct atctaaagaa ttcatcgatc tctatcaaaa
     3361 taaataggaa cactgtcatt cccatctgtc tttattaatg acgactacat tgtaggaacc
     3421 tccaaaagtt ggacaaaatg attgaaagga ttggatcctg cattacacaa gatccaatcc
     3481 ttttctttta ctctagtttt tataatatgt tgaatc
//
LOCUS       DAVMHG010000151         3501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVMHG010000151 DAVMHG010000000
VERSION     DAVMHG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.153"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..179
                     /locus_tag="VYR08_001887"
     CDS             <1..179
                     /locus_tag="VYR08_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV3467366.1"
                     /translation="ENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVT
                     LITGKNGTGKSTLL"
     gene            complement(545..1855)
                     /locus_tag="VYR08_001888"
     CDS             complement(545..1855)
                     /locus_tag="VYR08_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV3467367.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2092..2964
                     /locus_tag="VYR08_001889"
     CDS             2092..2964
                     /locus_tag="VYR08_001889"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV3467368.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3095..3492
                     /locus_tag="VYR08_001890"
                     /pseudo
     CDS             3095..3492
                     /locus_tag="VYR08_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1108 a          591 c          770 g         1032 t
ORIGIN      
        1 atgaaaacag aactgtttct tttgctatta gttcaaaagg agaaaaaatg aaagtagaaa
       61 atatttcgta tagggtggat catcgtatat tgtttgataa tatttctttt gatacttcga
      121 gttcaggcgt gacattaatt actggtaaaa atggtacagg aaagtcaact ttactatagt
      181 agattgaaac tagaatagta cacatctact tctaaaatat tgttagaaat cgatttgact
      241 atcctgatct atttgtcctg ttcttatttc atttcactat atctcaaatt gagtatgacg
      301 aagtgcgctc ccatgtcctg ggaacgcact ttcttcatat ttttcatatt cttgaatcca
      361 tcgataaaga ctattgggat gaatttttaa agttgaacta atcattttta caggatgaga
      421 tttacagcag agagtttgaa ggctttatca aaggtttttc ttggcataat gacttttcct
      481 cgtttccact taattttgtg tctactttat tataccaagt ccacccttaa gttagataat
      541 aaatctaact taaggaagct agaaggatga gaatccaggt ggtcaagagt cccaaactta
      601 agctgatggg gacacccaga ataatttgct ttttgaaggc aaggccacgt tcctctatat
      661 tgggaagtga gagttgaatg agagaaccag ctgatgaaaa gggtgagata ttagtagata
      721 gagcgccaat aacggtggct gttgtgagta agtgaatatc aatctgagga ttttgagcac
      781 tgatgatagc aatgatggga aagagggctg gagctacaac ggatagggtg gaactaaaga
      841 gtgacatcac tccggctatc acacaaaaga acagaggtaa ccagaaatga ggaatggttg
      901 ttgtcatgag gtgccctatc agtgtgacta aacctgactt gaccgctaga gacattagta
      961 agctcatgcc gcagagcatg ataattgtag cccagggaac cttagctaaa atggcttctt
     1021 gcttccctaa tttgagcctt aaggcgaggc agaccatgag tattgagaca aagccaatat
     1081 caaatgtttt ttgataagta gctatccagg cgatgtttgg gaaaatgaga tgcaacaagg
     1141 gaaaaagcca aaccaaaacc atgctgctga tcatgagcaa ggtggtttgt ctttgaacct
     1201 tgctgaggag tggtggttgg tcaatagtca aggatgagtt tgttcttccc ttactatagt
     1261 ggctgtaaca ggataataaa agcaagacga tgagtgggta gataatgctg acgataaaga
     1321 tatgattgcc aagtgaaaaa gcttgctctt cccatcccat ttgcttaaac aggccttgaa
     1381 agacaatgcc tgagctactg gttatcaaat tagcccctcc tgaagctccc caattgacgg
     1441 cttgagctcc aatcaaaggg tgtttgtccg ctttttgaca gagggtaatc gctagaggac
     1501 agcaaacggc catagtagtg aaaaatccag cacctaaagc agacaaaagg gttgccatca
     1561 ggtataaaat catgtagagg gcgttagggt gggtgcgtgt gcggtagaga atgtgttgag
     1621 ccaaaacatc aagagtaccg ttagttgttg caacgttata aaagagagag acgctaaaaa
     1681 tggtaaaaaa gagtgaggtt ggccaaaaat gaagaagttc tttggggctt aatcccatga
     1741 gagtggttgc gatgaggtaa gaaaaagcaa tagccagcag gccaatattg attttggtgc
     1801 ggtaaccaat tccaatggct agagcaatgg cgctaatcat tattaaatga atcattgaat
     1861 tgtcctttca gttagaatat ggaaatagat tggcaatttt ggcgctcaaa agaccacctg
     1921 tttcctattg gtacttgact attatacaca aaaaaaatac aattgtcttg acaattgtat
     1981 tgacacttgt tagaattaaa aatgaggtat tcaggcttgt gatttcctat ggaagttaat
     2041 agtaattgcc tctagtgctt acaagtgata ttaaaaatag aggacttagt gatgtcaatc
     2101 atttcaactg atttaacccc ttttcaaata gatgatacat tgaaagcagc cttgcgagaa
     2161 gatgttcatt ccgaagatta cagtaccaat gccatttttg atcatcatgg ccaagccaag
     2221 gtgtcgcttt ttgccaagga agctggtgtt ttagcgggat taaccgtttt tcaaagggtt
     2281 tttaccctat ttgatgccga ggtgaccttc cagaatcctc atcaatttaa ggatggggat
     2341 cgtttgacta gtggcgattt ggttttagaa atcataggct cggtgagaag tctcttaaca
     2401 tgtgaacgcg ttgccttgaa ttttttacaa catttatcag ggattgcttc gatgacagct
     2461 gcttatgtag aagccttagg cgatgattgc attaaggtat ttgatactcg aaaaactact
     2521 cctaatttac gtctttttga gaaatatgcc gtgagagttg gcggtggcta taatcatcgc
     2581 tttaatttat cagatgctat catgctaaaa gacaatcaca ttgcggcagt aggtagtgtt
     2641 caaaaggcaa ttgctcaagc gcgtgcctat gccccttttg tgaaaatggt cgaggtggaa
     2701 gtggaaagcc ttgctgctgc cgaagaagct gcggcggcgg gtgctgatat tatcatgttg
     2761 gataatatgt cattggaaca gattgaacag gctattaccc taattgcagg acgttctcgg
     2821 attgaatgtt ctggaaatat tgatatgacc actattagcc gttttcgtgg tttagcgatt
     2881 gattacgtct ccagtggtag tttaacccat agtgctaaga gtcttgattt ttccatgaag
     2941 gggttaacct accttgatgt ctaagttgta aaataaacta actttttaaa ggatgttttt
     3001 cctctagaac gagttttatg tcagatagtt taaacgcctc ttcaaatata gtaaaatgaa
     3061 ccaaaaatag tacacaatgt ggtataatct ttttatggca tattcaatag attttcgtaa
     3121 aaaagttctc tcttattgtg agcaaacagg tagtgtaaca gaagcataac acgttttcca
     3181 aatctcacgt aataccattt atggctggtt aaagctaaaa gagaaaacag gagagctaaa
     3241 ccaccaagta aaaggaacaa aaccaagaaa agttgataga gatagactta aaaactatct
     3301 tactgacaat ccagatgctt atttgattga aatagcttct gaatttggct gtcatccaac
     3361 taccatccac tatgcgctca aagctatggg ctacactcga aaaaaaagaa ccacacctac
     3421 tatgaacaag agccagaaaa agtagcctta tttcttaaga attttaatag tttaaagcac
     3481 ctagcacctg tttagattga t
//
LOCUS       DAVMHG010000152         3498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVMHG010000152 DAVMHG010000000
VERSION     DAVMHG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.154"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..985
                     /locus_tag="VYR08_001891"
     CDS             <1..985
                     /locus_tag="VYR08_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467369.1"
                     /translation="KYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTLRALVGFLDP
                     SSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKKVAPDVIKAK
                     VSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPLSNLDAKLRV
                     DLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPVEIYHNSQTE
                     FVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQDFILKGTII
                     DVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVLQF"
     gene            997..2688
                     /locus_tag="VYR08_001892"
     CDS             997..2688
                     /locus_tag="VYR08_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3467370.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            2691..3401
                     /locus_tag="VYR08_001893"
     CDS             2691..3401
                     /locus_tag="VYR08_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV3467371.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1064 a          512 c          667 g         1255 t
ORIGIN      
        1 taaatatggt gattttgttg caattgataa tctgaattta gatatacatg aaggggaatt
       61 ctttacattt cttgggcctt caggatgtgg taaatcaact actttgagag cattggtagg
      121 ttttttagat ccatcatcag gaagtattga agttaatgga acagatgtca ctcatttgga
      181 acctgaaaag cgtggaattg gtattgtatt tcaatcttat gcgctatttc caactatgac
      241 tgtttttgat aatattgcat ttggtttaaa agttaagaag gtagctccag atgttattaa
      301 agctaaagta tcagcagtgg cagcaaaaat taagatctct gatcaacagt tacagcgtaa
      361 tgtatcagaa ttatctgggg gtcaacaaca aagggtagca ttggctcgtg ctctggttct
      421 tgaacctaaa attctttgtc tagatgaacc attgtcaaac cttgacgcaa aattacgtgt
      481 agatttgaga aaagagttga aaagacttca aaaagagtta ggtattacta ctttatatgt
      541 tactcatgat caagaggaag ccttgacttt atctgataga attgcagtct ttaacaatgg
      601 atacatcgaa caggtcggta caccagtaga gatttatcat aattctcaaa ctgaatttgt
      661 atgtgatttc attggagata ttaatgtttt gaccgatgaa acagtccacg aagtattatt
      721 gaaaaataca agcgttttct tagaggataa aaaaggatac attcgattag agaaagttcg
      781 attcaatcgt gaaactgaac aagattttat tctaaaaggg acaattattg atgttgagtt
      841 ttctggagtt acaattcact atacaataaa agtttctgaa agtcagattc ttaatgtaac
      901 aagtattgat agtcaggctg ctattagatc tgtcggagaa agtgtggaat tatttatcac
      961 accatcagac gttctgcaat tttaaggagg tgcagtatgc gtcataaatt aaatttaaaa
     1021 gattggctta ttcgtttagg gttaatctgg ttcttagtaa catttattat ttatccaaac
     1081 tttgatctag tagtgaatgt atttgtaaaa ggaggagaat tttcccttga tgctgtacat
     1141 cgtgttctaa aatctcagag ggcacttcag agtattatga acagttttaa gttagcattt
     1201 tcacttatta ttacagttaa tgtcgtaggt attctttgtg ttctatttac agagtacttt
     1261 gatattaaag gtgctaaaat tttaaaatta ggttatatga cctctttaat ttatggagga
     1321 gtggttttag cgactggata taaatttgtc tatggtcctt atggattgat tacaaaattt
     1381 ttacaaaatg ttatcccttc tttagactct aactggttta ttgggtatgg tgcagtctta
     1441 ttcattatga cattttcagg aactgctaat catacattgt ttttaacaaa tacaattcga
     1501 agcgttgact atcacactat tgaggctgct cgaaatatgg gagcaaaacc atttactgtt
     1561 ttccgaaaag tagtgttacc aaccttaatt ccaactctat ttgcacttac tattatggtt
     1621 tttcttagtg gtttatctgc agtagcagca cccatgattg ttggtggtaa agaatttcaa
     1681 actataaatc caatgattat tacatttgca gggatgggga attctcgtga tttagctgcc
     1741 ttacttgcaa ttattttagg tattgcaact acaattttgc ttactatcat gaataagata
     1801 gaaaaaggtg gaaattatat ttctatctct aagactaaag cgcctcttaa aaaacaaaaa
     1861 attgcgtcta agccttggaa tatcattgct cacattgtag catatggatt gttcacagtt
     1921 ttcatgcttc cactaatttt tatagtatta tactcattta cagatccagt tgcaattcaa
     1981 acaggtaact taacattatc aaactttact ttagaaaatt atcgcttatt ctttagtaat
     2041 agtgcggcat tctctccatt cttggtcagc tttatttatt ctattattgc tgcgacaaca
     2101 gcaacaattc tcgcagttgt atttgctcgt gttgtcagaa aacataaatc tcgttttgat
     2161 ttcttatttg aatatggtgc tctacttcct tggttactac caagtacact tttagcagta
     2221 agtttattat ttacttttaa tcagccacaa tttcttgtct tgaatcagat tttggtaggt
     2281 agtttggtaa ttctacttat tgcatatata gttgtaaaaa tcccattttc ttatagaatg
     2341 gtacgtgcta ttttatttag tgttgatgat gagatggaag atgcagcaag aagtatgggt
     2401 gcttcacctt tttatactat gatgaaggtt atcattccat ttattttacc ggttgttctc
     2461 tctgttattg ctttaaactt taactcttta ttaactgact tcgacttatc tgtattcctt
     2521 taccatcccc tagctcaacc attaggtatt acgattcgat ctgcaggtga tgaaacagca
     2581 acatctaatg cacaagctct ggtatttgtt tatacaattg ttctgatgat tatttctgga
     2641 acggtattat acttcacaca aagaccgggg cgtaaagtaa ggaaataatc atgacagcca
     2701 ctagtcttgg gttatcaaat attgaaatag ttgtcaggat tgttttatca gtagtcattg
     2761 gtagtataat tggtttagag agagggagca aatcccagcc tgcaggcatc cgaacttata
     2821 gtattgtttg tctagctgca tgtttgatta tgatgacgaa tgaatacgta tcttataaat
     2881 ttgggacagg agatcctaca cgattaggag ctcaagttat atcaggtgtg ggttttctag
     2941 gcgctggaac gattcttatt acagataaaa agaaaattac aggtctgaca actgcagcag
     3001 gcatttgggc ttcggcagga attggattag ctattggagt aggtttttat gagggagctc
     3061 ttttagtagc catttctgtt tggggtgtga tatccatgtt ccaaccacta aaaaaatatc
     3121 tgcaaaatcg ttctaaaatg attgaattgt atatagtggt taaatctaca gaagcctata
     3181 atcgtgtatt ggtttattgt gcagaaaatg gtatcagaat gaccgattca agaacagcgt
     3241 ttggagatgt taattctgac agaattgaat actttgatgt tccggacaaa aaaatagcgt
     3301 catttattac tctggaatta tcaggtagat ttgaacatct tcgactgatg gaagaaattg
     3361 ctaatattgt aggtgttatt tatgtcgaag aaattagctg aaatttagta aacaaaggga
     3421 cagatgtttt tgtccttttg ttttttagat gttttaatca tctaattttt gagtagattt
     3481 ttgctcagag gtttttat
//
LOCUS       DAVMHG010000153         3465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVMHG010000153 DAVMHG010000000
VERSION     DAVMHG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.155"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..1283
                     /locus_tag="VYR08_001894"
     CDS             105..1283
                     /locus_tag="VYR08_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV3467372.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            1507..2286
                     /gene="rpsB"
                     /locus_tag="VYR08_001895"
     CDS             1507..2286
                     /gene="rpsB"
                     /locus_tag="VYR08_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV3467373.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            2365..3405
                     /locus_tag="VYR08_001896"
     CDS             2365..3405
                     /locus_tag="VYR08_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV3467374.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         1200 a          628 c          749 g          888 t
ORIGIN      
        1 attatctata agaacgacat ataaatgtaa caaaggcgta atatttatta ggcctttttt
       61 tggtatacta gtattgtctt taaaagaagg agtatctacg taatatgaag aaaaaaatct
      121 tagcgtcact tttattaagt acagtaatgg tttctcaagt agctgtttta acaactgcgc
      181 atgcagaaac gactgatgac aaaattgctg ctcaagataa taaaattagt aacttaacag
      241 cacaacaaca agaagcccaa aaacaagttg accaaattca ggagcaagta tcagctattc
      301 aagctgagca gtctaacttg caagctgaaa atgatagatt acaagcagaa tctaagaaac
      361 tcgagggtga gattacagaa ctttctaaaa acattgtttc tcgtaaccaa tcgttggaaa
      421 aacaagctcg tagtgctcaa acaaatggag ccgtaactag ctatatcaat accattgtaa
      481 actcaaaatc aattacagaa gctatttcac gtgttgctgc aatgagtgaa atcgtatctg
      541 caaacaacaa aatgttagaa caacaaaagg cagataaaaa agctatttct gaaaaacaag
      601 tagcaaataa tgatgctatc aatactgtaa ttgctaatca acaaaaattg gctgatgatg
      661 ctcaagcatt gactacgaaa caggcagaac taaaagctgc tgaattaagt cttgctgctg
      721 agaaagcgac agctgaaggg gaaaaagcaa gtctattaga gcaaaaagca gcagctgagg
      781 cagaggctcg tgcagctgcg gtagcagaag cagcttataa agaaaaacga gctagccaac
      841 aacaatcagt acttgcttca gcaaacacta acttaacagc tcaagtgcaa gcagtatctg
      901 aatctgcagc agcacctgtc cgtgcaaaag ttcgtccaac atacagtaca aacgcttcaa
      961 gttatccaat tggagaatgt acatggggag taaaaacatt ggcaccttgg gctggagact
     1021 actggggtaa tggagcacag tgggctacaa gtgcagcagc agcaggtttc cgtacaggtt
     1081 caacacctca agttggagca attgcatgtt ggaatgatgg tggatatggt cacgtagcgg
     1141 ttgttacagc tgttgaatca acaacacgta tccaagtatc agaatcaaat tatgcaggta
     1201 atcgtacaat tggaaatcac cgtggatggt tcaatccaac aacaacttct gaaggttttg
     1261 ttacatatat ttatgcagat taatttacag agggactcga atagagccct cttttcaggt
     1321 tttaccgtga caatccctat taaaaattat atcaaaatag cttgaaaata ttggaaaagt
     1381 atggtaaaat gaaaattgtc gtgtgaacga taatactcat tcttgatgaa ttgtgaagca
     1441 gttgcccttg ggtcgttttg cgagttgaag tcaagaagag gaaaaaaaca aaaaggagaa
     1501 atactcatgg cagtaatttc aatgaaacaa cttcttgagg ctggtgtaca ctttggtcac
     1561 caaactcgtc gctggaatcc taagatggct aagtacatct ttactgaacg taacggaatc
     1621 cacgttatcg acttgcaaca aactgtaaaa tacgctgacc aagcatacga cttcatgcgt
     1681 gatgcagcag ctaacgatgc agttgtattg ttcgttggta ctaagaaaca agcagctgat
     1741 gcagttgctg aagaagcagt acgttcaggt caatacttca tcaaccaccg ttggttgggt
     1801 ggaactctta caaactgggg aacaatccaa aaacgtatcg ctcgtttgaa agaaattaaa
     1861 cgtatggaag aagatggaac tttcgaagtt cttcctaaga aagaagttgc acttcttaac
     1921 aaacaacgtg cgcgtcttga aaaattcttg ggtggtatcg aagatatgcc tcgtatccca
     1981 gatgtgatgt acgtagttga cccacataaa gagcaaatcg ctgttaaaga agctaaaaaa
     2041 ttgggaatcc cagttgtagc gatggttgac accaatactg atccagatga tatcgatgta
     2101 atcatcccag ctaacgatga cgctatccgt gctgttaaat tgatcacagc taaattggct
     2161 gacgctatta tcgaaggacg tcaaggtgag gatgcagtag cagttgaagc agaatttgca
     2221 gcttcagaaa ctcaagcaga ttcaattgaa gaaatcgttg aagttgtaga aggtgacaac
     2281 gcttaattta tacaaatagt aattacctag gagggcgggg cttagcccgg ctctcctatt
     2341 ttcaaaaaat ataggagaat taaaatggca gaaattacag ctaaacttgt aaaagagttg
     2401 cgtgaaaaat ctggtgccgg tgttatggac gctaaaaaag cgcttgtaga aacagacggt
     2461 gacatcgaaa aagcgattga attgcttcgt gaaaaaggta tggctaaggc agctaagaaa
     2521 gctgaccgtg ttgctgcaga aggtttgact ggtgtttatg ttaacggtaa tgttgcagca
     2581 gttattgaag taaacgctga aactgacttc gttgcaaaaa acgctcaatt cgttgaattg
     2641 gtaaatacta cagctaaagt cattgctgaa ggaaaacctg ctaacaacga agaagctctt
     2701 gctttgataa tgccttcagg tgaaactctt gaagctgcat acgtatctgc aacagcaact
     2761 atcggagaga aaatctcatt ccgtcgcttt gcattgattg aaaaaacaga cgcacaacac
     2821 tttggagcat accaacataa cggtggacgt atcggtgtta tttcagttgt tgaaggtgga
     2881 gacgaagcac ttgctaaaca attgtcaatg cacatcgcag cgatgaaacc aacagttctt
     2941 tcttacaaag aattggatga gcaattcgtt aaagatgagt tggcacaatt gaatcacgtt
     3001 atcgaccaag acaacgaaag ccgtgcaatg gttaataaac cagctcttcc acacttgaag
     3061 tatggatcaa aagctcaatt aactgatgat gttattgctc aagctgaagc tgacatcaaa
     3121 gctgaattgg ctgcagaagg caaaccagaa aaaatctggg acaaaattat tccaggtaaa
     3181 atggatcgct tcatgcttga taacactaaa gttgaccaag cttacacact tcttgcacaa
     3241 gtttacatca tggatgacag caagacagtt gaagcatacc ttgaatcagt taacgcttcg
     3301 gtagttgagt ttgctcgctt tgaagttggt gaaggtatcg agaaagctgc aaacgacttc
     3361 gaagctgaag ttgcagctac aatggcagca gccttgaata actaataata gaaaggaagc
     3421 aaattgcttc cttttttgta tagggcttag atttttcctg aaatt
//
LOCUS       DAVMHG010000154         3423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVMHG010000154 DAVMHG010000000
VERSION     DAVMHG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.156"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            89..1150
                     /gene="dinB"
                     /locus_tag="VYR08_001897"
     CDS             89..1150
                     /gene="dinB"
                     /locus_tag="VYR08_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV3467375.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSN
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1297..2142)
                     /locus_tag="VYR08_001898"
     CDS             complement(1297..2142)
                     /locus_tag="VYR08_001898"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV3467376.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2398..>3423
                     /locus_tag="VYR08_001899"
     CDS             2398..>3423
                     /locus_tag="VYR08_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV3467377.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNI
                     HAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFK
                     AYLKTKLE"
BASE COUNT         1101 a          704 c          676 g          942 t
ORIGIN      
        1 gcagattgct caaaacactg ttttgaggtt gcagatagag ctgacgtggt ttgaagagat
       61 ttttgaaaag tataaaaagg tgctaggcat gttgattttt cctttgttaa atgatttgtc
      121 aagaaaaatc atccatattg acatggatgc cttttttgct gcagtggaaa tcagggataa
      181 tcctaaactc agaggaaaac ctgtcattat tggaagcaac cctcggcaaa caggtggacg
      241 gggagtcgtt tctacctgta gttatgaggc aagagctttt ggtgtccatt ctgccatgag
      301 ttccaaggaa gcttatgaac gttgtcccca ggctgtcttt atctcaggga attatgagaa
      361 atacaagtct gtgggactcc agattcgagc tatttttaag cgctatacag atttgattga
      421 acccatgagt attgacgaag cctatttgga tgtgacagaa aataaactcg gtatcaagtc
      481 agcggtcaaa attgctcgcc tcattcaaaa agatatctgg caagaactcc atctaactgc
      541 ttctgccggt atttcctata acaagttctt agctaaaatg gcgagtgatt atcaaaaacc
      601 tcatggtttg acagtgattc tacctgaaca ggctgaggat tttctcaaac aaatggatat
      661 ttccaaattt catggagtag gaaaaaagac agtagaacgt cttcatcaaa tgggcgtttt
      721 tactggtgct gatttacttg aagttcctga ggtgacccta atagaccgtt ttggtagact
      781 aggctatgat ctgtatcgaa aggctcgtgg cattcacaac tctccagtca aatccaatcg
      841 catccgtaaa tcaatcggca aggagaaaac ctacgggaag attctccgtg ctgaggaaga
      901 tatcaaaaaa gagctgactc ttctatcaga aaaagtcgct ctcaatctac atcaacaaga
      961 aaaagctgga aaaattgtca ttttgaaaat ccgctacgag gacttttcaa ctcttaccaa
     1021 acgaaaaagt cttgctcaaa aaacacaaga tgctagtcag ataagccaaa tagccctgca
     1081 actctatgaa gaattaagtg agaaagaaag aggtgtccgc ctattgggga ttaccatgac
     1141 tggattttaa agctcaatga aaatcaaaga gcaaactagg aggctagccg caggctgctc
     1201 aaaacactgt tttgaggttg cagataaagc tgacgcggtt tgaagagatt ttctaagagt
     1261 ataaaacctt gaagagttgc cccttcaagg tttttcttat acaaataaac ggacaaaact
     1321 gtaaagtagc aaaacactac caagcacgat acggtattta ccaaaaaggg tgaagtcgtg
     1381 ttttttcaca tagctggtca agaagcgaat agccaccatg ctgaccgcaa aagctactcc
     1441 catcgcgacc aagagcaaaa acaattgccc aaagctcaag agttctccgg ctttcacaaa
     1501 tttgaaaatc tttaaggcac tagctccaaa cataacagga atcccaagat agaaggtaaa
     1561 ttctgtcaca actgaacgac tggttccatt taacaaacca ccgacaatcg ttgcacctga
     1621 acggctagtc cctggtaaaa gagcaagaac ttggaagagt ccgatataga aagcggtcgt
     1681 ataaggaagc ttgtccaact ctgttacact tggctcgata gcacgcgctt tattgcgctt
     1741 ttccaaatag atgaaggcaa tcccgtagat aatcaacatg agagcaactg aaaccatgtt
     1801 atggaagtgg gtatcaaacc aatcatcaaa tttaaagaca ccaagtaaag gtaaagtagc
     1861 aatcaagacc ttcaaccata gtctccaagt cttacgaact tcctgtttgt ccttagtcgg
     1921 tttaaaagga ttgagcttgt taaaataaat caccataact gctaaaatag caccaagctg
     1981 aatcacgaca ttaaacatgg acataaaggc ttcattttga ttttggtatt ggataaactc
     2041 ctctgctaaa atcaagtgac ctgtactgga aatcggcaac cattccgtaa ttccttcaac
     2101 aatcccgaag aagatagatt ttaaaatttc aataagatac atatatttct ccttatatta
     2161 tatttaacgc agttagctac tgccaaagcc caagtggtat acttggaata agccactgtg
     2221 gattagttca tttcctccaa tcgcttgacg atttccgaaa gggtctaacc acagcttttt
     2281 gtttcaattg gtaatgagtt tgataacgca tagacgtttt atctcgtcta aggtaacaag
     2341 gcaaaaagtt cgtggataac caagtagact tgaaatacta tatctggagg tactcccatg
     2401 ctttatgtgg gcattgatgt cgctaaaaat aaacacgatg ttacagcctt gaatgttcca
     2461 ggaaaaactg ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta
     2521 gatctgtctt ttcgacagct caaccaagac tgtctcatcg ctcttgaaga tactggacat
     2581 tatgccttta atctactgaa cttcctgcat gaacaaggat ataaagttta cacctacact
     2641 cctttgctca tcaaggaatt tgccaaatca ttatcacttc gcaagactaa aacggataag
     2701 aaagacgctc atggcatcgc tcttaaactt ctttctgacc ccaatcgtga acaatttcaa
     2761 cacgataatc ggcaagtaga actaaaaata ctggctagac atattcatcg tctcaagaaa
     2821 aaacagtctg attggaaagt acaatacact cgttgtcttg atatcatctt tcctgagttg
     2881 gataaaatcg ttggaaagca ttcagaatat acctaccaac tcttgacatg ctaccctaat
     2941 cctcagaaaa ggcttgaggc aggatttgat aagctgatag aaattaagcg attgaccgct
     3001 tctaaaattc aggatatcct ctcagtcgca cctcgttcta tcggaacaac atctcctgct
     3061 cgtgaattcg aaatcatcga aatcatcaaa cattacaaga ggctcattga caaggcggaa
     3121 acatgtgtca atgacttgat ggctgagttc aactcggtca tcacgacggt tactgggatt
     3181 gggggtcgtt taggggcggt cattttagcc gagattcgaa atattcatgc ctttgataat
     3241 cctgctcaat tacaagcttt cgctggactg gattcttcta tttatcagtc aggtcagatt
     3301 gatttagctg gaagaatgat caaacggggt tcccctcatc tgcggtgggc actcatacaa
     3361 gctgccaaag catgcgctcg cttttcacct gcttttaagg cctatcttaa gactaagtta
     3421 gaa
//
LOCUS       DAVMHG010000155         3420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVMHG010000155 DAVMHG010000000
VERSION     DAVMHG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.157"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(131..385)
                     /locus_tag="VYR08_001900"
     CDS             complement(131..385)
                     /locus_tag="VYR08_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV3467378.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            complement(405..2246)
                     /gene="typA"
                     /locus_tag="VYR08_001901"
     CDS             complement(405..2246)
                     /gene="typA"
                     /locus_tag="VYR08_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV3467379.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2408..3133
                     /locus_tag="VYR08_001902"
     CDS             2408..3133
                     /locus_tag="VYR08_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV3467380.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(3173..3400)
                     /locus_tag="VYR08_001903"
                     /pseudo
     CDS             complement(3173..3400)
                     /locus_tag="VYR08_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1022 a          750 c          637 g         1011 t
ORIGIN      
        1 gatgccatta acgaatttta ttcaagtttt tcccattcat attatccaaa caaaagagat
       61 ggtgataact caaaaataaa ttgcaatctt gtaaaattcc tagcaaaaaa gagagccaaa
      121 actctctttt ttatcttctt ttactttttt tgactggcat gagtgtaatg tctctaacac
      181 taaagtaagc taggagcaac atggctattg ctaggaatat ttctgttggt aattgaaaaa
      241 ttttcagaaa agatagaacc aataaaatca agagtgccac taaaatacat accatagcga
      301 cgatattgac tgtcccttta atgctttctg gtgtcgcaaa tacatagagt aggagcagta
      361 aaattcctag gactaaatag accatctttc tctctttcta gctcttattc agctgatttt
      421 ttcttcttgt tagctttctc acgctctgct ttgttaagga tttgtttacg caaacggata
      481 gactcaggcg ttacttccat gtactcatcg tcgttcaaga actcaagaga ctcttcaagt
      541 gtcaagatac gaggcgtctt gataacagct gtttggtcct tagtagctga acgaacgttg
      601 gtcatttgtt ttgccttcgt gatgttaact gtcaagtcat tttcacgaga gttttcaccg
      661 atgatcattc cttcataaac ctcagtacct gggttgacaa agatcgtacc acgttcttcg
      721 atagacatga ttgagtaagt tgtagcctta ccagcatcga tagaaacaag ggcaccacgg
      781 tgacgtccac caatttcccc tggaatcaat ggcaagtatt ggtcgaaggt atggttcatg
      841 ataccgtaac cacgagtcat tgacaagaac tcagttgagt atccaatcaa accacgcgct
      901 ggaacaagga agaccaaacg agtttgacca ttaccagttg aaatcatatc caacatttca
      961 cctttacgtt cagaaaggct ttggataaca gacccttggt attcttctgg agtgtcgatt
     1021 tgtacacgtt caaatggttc acatttaaca ccgtcgattt cttttacgat aacttctgga
     1081 cgagatactt gaagttcata gccctcacga cgcattgttt cgataaggat tgacaagtgc
     1141 aattctccac gtcctgaaac agtccattta tctggtgaat cagttgggtc aacacgaagg
     1201 gaaacgtctg tttgcaattc tgcctgcaag cgttcttcca ccttacgaga agttacccat
     1261 ttaccttctt taccagcaaa tggtgagttg ttgaccaaga aagtcatttg aagagttggc
     1321 tcatcgatgt gtaggattgg aagagcttct actgcatctg tcggagtgat ggtttcaccg
     1381 acaaagatgt cttccatacc tgaaacggca atcaagtcac ccgctttggc ttcttggatt
     1441 tcacgacgtt ccaaaccaaa gaaaccgaag agttttgtaa cacggaagtt tttagttgta
     1501 ccgtcaagtt tagaaagggt aacttggtcc ccaaccttaa ctgtaccacg gaagacacga
     1561 ccgataccga tacgtccaac gaagtcattg tagtccaaaa gtgacacttg gaactgcaaa
     1621 ggctcatctg agttatctac tggagctggg atatggtcga taatcgtgtc aaagattggt
     1681 gccatagtcg cttcttggtc agctggatca tctgacaatg aagaagttcc gttgatcgct
     1741 gaagcataaa ccactgggaa atcaagctgg tcgtcatctg caccaagctc gatgaaaagt
     1801 tccaagactt catccactac ttctgctgga cgagctgatg gcttatcgat tttgttaaca
     1861 accacgattg ggacaaggtc ttgttccaag gcttttttca atacgaaacg agtttgtggc
     1921 atggttcctt cataggcatc tacgaccaag acaacaccgt caaccatttt catgatacgc
     1981 tcaacttctc caccgaagtc cgcgtgtcct ggtgtgtcca taatgttgat acgagttccg
     2041 ttgtaagcaa cggcagtatt tttagcaagg atggtgattc cacgctcttt ttcgatatcg
     2101 tttgagtcca tagcacgctc tgccaattca gtacgtgcat caagcgtttc tgattgtttc
     2161 aataattcgt caaccagggt tgttttaccg tggtcaacgt gggcgataat cgcaatgtta
     2221 cggatatctt ctcttaattt tgtcatgatt tcctctataa tattcaaaat ttattttcta
     2281 actgaacgat tataccataa tttcaaataa ataacataac tcaagcaagt gtaaatgttt
     2341 tcactccgct tttcttttca cgtcaagcct tttcaaagca agcgacttat gataagatag
     2401 gcacagtatg cgtttagata atttattagc ccaagaaaaa atcagccgaa aggccatgaa
     2461 gcaagcactc ctcagaggga aaattctagt cgatggttgc ccagcccgct ccctagctca
     2521 aaatatcgat acaggactac aagaactcct ttttcaggat cgaatcattc aaggctatga
     2581 acacacctat cttatgcttc ataaacctgc tggtgtcgtt acagccaaca aagacaagga
     2641 acttccgacc gtcatggacc tgcttccatc tgacatccag tctgacaagc tctatgccgt
     2701 tggccgactg gaccgagata caacgggact cctcctcttg accgataacg gtcccttggg
     2761 cttccaactc ctccatcccc aatatcatgt cgataagact tatcaagttg aggttaatgg
     2821 acttctaaca cctgaccata tccaagcctt tcaaaaggga attgtctttt tagatggtac
     2881 tgtctgtaaa cccgcaaaac tagagattct atctgcaagt ccctccatca gtcaagcctc
     2941 tatcaccatt tcagaaggaa aatttcatca aatcaagaaa atgttcctct cggttggtgt
     3001 taaggtgact atcctcaaga gaatccaatt tggggacttc acattgaacc cagatttagc
     3061 agaaagtaac taccgccctt tgaaccaaaa agagttacaa atcattaaaa actatttaga
     3121 gatgagtcga taaaacaaaa aaagctttaa aactaaagct tttttctttt atttaccgaa
     3181 aaattaaggc gattgctaca atccagttaa ctacagaaat cacaattcct aagatattaa
     3241 gaatcttttc tattttatag tctaattatg actctttttg gtatgaaata gccaagacca
     3301 atcctatgat acccaaaatc aggcctacaa ttggaaataa caaaccaaga ataatcgaca
     3361 agatacccac aaaaagtgga tttttcttct tttcttttat gttctaagaa ctccttaaat
//
LOCUS       DAVMHG010000156         3183 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVMHG010000156 DAVMHG010000000
VERSION     DAVMHG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.158"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(105..1520)
                     /locus_tag="VYR08_001904"
     CDS             complement(105..1520)
                     /locus_tag="VYR08_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV3467381.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPQGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1533..>3183)
                     /locus_tag="VYR08_001905"
     CDS             complement(1533..>3183)
                     /locus_tag="VYR08_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV3467382.1"
                     /translation="ILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEYIAGSM
                     TPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAYSASQK
                     LKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMVWLMKY
                     IEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMYGVAGW
                     LTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAASMAVAL
                     KSKNNNTKQIAFAASFSALLAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFAGLTSV
                     KAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEESSSNLE
                     VKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFDGKITN
                     IFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEMDLNLI
                     KTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
BASE COUNT         1080 a          639 c          439 g         1025 t
ORIGIN      
        1 tataaaataa agaggtttag aatttgatgt cttccaaacc tctttatttc atattcaatg
       61 agatgttgcc ttaaccgtga taatagctag tcatcaataa aaaactattt gaataaggat
      121 tctccatttg attcaatcac ttctttatac caagtaaaag acattttctt atatcgattt
      181 aatgtaccac ttccatcatc atttcgatca acataaatga gaccgtacct tttagaaagt
      241 tgtgcagtgg acatagaaac acagtcaata catccccaag acgtatagcc cataatttcc
      301 acaccatctt gtagagcttc agctacttgc aataaatgtt ctttcatata ctgaattcta
      361 taatcatctt ggacggttaa gttattaagt tcatctttta ttagttgatc tttagcacct
      421 aatccatttt ctactataaa taatgggatt tgataacggt cataatatct atttaaaatt
      481 atacgtagtc caattggatc aatttgccat ccccactctg aagactctaa ataaggattt
      541 actaaaccac caataatatt cccttctcct gaattatact gtgttggaag agcagattga
      601 gtcacactca tataatagct aaaggataaa aaatctacag tataattttt taataactct
      661 gcatcttcag ctgcaaactc tatgttaatg tcattttcct taaaatatct ttttgcataa
      721 ttcggataat aacctctaac atgcacatct gaaaatagat aatttagatt ctcatactca
      781 tgagtcgccc atacatcttt tggatttgga gtcattggat aagctggcat agctaatacc
      841 atacatccca ccttaaactc tgaattaatc tcacgagcaa tttttgtaac caaacttgag
      901 gcgactaatt catgatgtat agcttgatat aattcttgtt tcgaaagatt ctccttaggt
      961 atatctattc ctccactagt aaatggtaat tccaaaacag agtttacttc gttaaatgta
     1021 agccaatatt taactttatc tttatacctt tctaaaactg ttcgagcaaa tttttcataa
     1081 aaatgaatca ttctcctatc aacccatcca tgatattttc ttgctaaata taatggagtc
     1141 tcatagtgtg aaagagttac aagtggctct atcccgtgag catgtagttc atcaaacaat
     1201 tcatcataat atttcaaacc agcttcgtta ggttcttctt catctccttt tggaaaaatt
     1261 ctactccatg caatagaagt acgaaaaaca ttaaagccca tttcagaaaa caaggatata
     1321 tcttccttat atttatgata aaaatcaata cctatcaatt ttaagttatc ttctgtagga
     1381 ttttctgttg cttctcctaa tccaccttgg ggtaacacat cctgaactga taagccctta
     1441 ccatcttcat tatatgctcc ctctacttga ttagctgcaa cagctccacc ccaaagaaaa
     1501 tcatctggaa aaatggtcat aactttcctc cattataata ttaccagtaa ttccttagaa
     1561 tgctcgattg tctgattatt aggtaatact aatacatcta gaaaatcatt ggtattcgtt
     1621 acaattactg gtgtaactgt ttcgtagcct ttagtcttga ttaaattcaa gtccatttca
     1681 aaaatcaact gatttttgaa aactctgtct ccttcttcta catgactaat aaaaccttga
     1741 ccttttagct caacagtatc taatccaata tgaattagta actcaacacc ctcatcactc
     1801 ttcaatccaa ttgcgtgctt agtcggaaaa atatttgtaa ttttcccatc aaatggtgca
     1861 taaaccttac cttcacttgg gataatcgct actccgtctc caattagttt atctgaaaat
     1921 gttttatcct ggacatcgct taacggaatg atttctcctg atataggaga aaatatcatt
     1981 tttttatttg aaactccagc tttaacttct aaattgctag aactctcttc ttcatcgatt
     2041 ccaaatatat aagctaatac aaaggtaata acaaccgaaa tgaccgccac aattaaagca
     2101 tttacaatat ttgatggcac atcagaataa ataaattgag gcaacgctat caaagatggg
     2161 acagcaaata gatacgcttt aacactagta agacctgcaa ataatcccgc taatccacca
     2221 ccaatcatag ctgcataaag cggtttttta tattttaaag taacaccata tactgcaggt
     2281 tcggtaatcc ctgcaagtaa ggctgagaaa cttgctgcaa aagcaatttg ttttgtatta
     2341 ttatttttac tctttaatgc aacagccatc gaagcagccc cttgagctaa gtttgaccct
     2401 aacattgctg gaagaattaa tacgtctgga gtagcaatag atgccgccaa aaaaataggt
     2461 gcaaaagccc aatgcattcc agtcataaca ataaatggca taatagcacc aagaatagct
     2521 aatgtaagcc atccagctac accatacatt tgcccaacta gatttgataa tccttcacca
     2581 acaattactc caataggtcc gactacaact aaggcaatac agcttgatac taataatact
     2641 agcgtaggtt gcaaaaaact cttagtaata gctggtgtta atttagcaat tattttttca
     2701 atatatttca tcaaccaaac cataataaga attggaacga ctgatgaacc ataactagct
     2761 ggtgtcacag gtgcaccaaa taaactaaga ggattccctg attgcaccat ttgaacaaaa
     2821 tttggatgga gaagtacacc tgctacagac atagctaatg tagatgttac ttttaatttt
     2881 tgtgatgcag aataagctaa taacagcggt aagaaataat atggagcatc cccaaaaaat
     2941 gtcaaaaaag caatagtctg agaatctgat tgcaatatac caagcattgg taaaataatt
     3001 accaagactt tcagcatacc tccccctaac attgctggaa tgattggagt catggaacca
     3061 gcgatatact caatgattct ttctaaaata ttccctttgt gcccttgaac aactgaatcg
     3121 gattcaaaat tgccaagttt aacgaattct ttataataat tagctacatc attaccaagt
     3181 ata
//
LOCUS       DAVMHG010000157         3162 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVMHG010000157 DAVMHG010000000
VERSION     DAVMHG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.159"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..314
                     /locus_tag="VYR08_001906"
     CDS             <1..314
                     /locus_tag="VYR08_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467383.1"
                     /translation="KKGQFDELVSNLNLSEQEQLDLLQQYKEEHPRRAKRGIKSAIIK
                     KVARFLAAKVGQKSVVEITDYLFEWQDNLEAGAENYLVQYGWDRNIAHWTIKTVSFIF
                     L"
     gene            801..1763
                     /locus_tag="VYR08_001907"
     CDS             801..1763
                     /locus_tag="VYR08_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEV3467384.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRE
                     TLIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTSQIVDEA
                     IKEQPKGRFLFLTLTVKNVPGKELNATISQLTQSFDRLFRRAKVKKNLIGFLRSVEVT
                     HNQEEETYHPHIHVLMMVKSSYFSGAGDNYVSQEEWGRMWEQSLKVDYVPIVDIRSVK
                     EIGKGLKGAILETAKYPIKPIKLDVENKQVVGDLYNGLYRKRQLGYGGLFKEIRKRLQ
                     LSNVENGDLVYISDDNDEMSKGTKIVAIWNATKQNYFVKNKGWN"
     gene            2268..2537
                     /locus_tag="VYR08_001908"
     CDS             2268..2537
                     /locus_tag="VYR08_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467385.1"
                     /translation="MSDEEFKEEAHQFRDKEIKILEEKLKELNISCKVVGINSIKDMN
                     EYKEIMENVNQAKEELKRIDELIAVRSSRIDFLEDKTLRITGNQR"
     gene            3000..>3162
                     /locus_tag="VYR08_001909"
     CDS             3000..>3162
                     /locus_tag="VYR08_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467386.1"
                     /translation="MNKKFLKCGTLFLISCSILGSTIPAVTVFSDEVTITYNSENNSE
                     KNELYNQLSA"
BASE COUNT         1115 a          365 c          651 g         1031 t
ORIGIN      
        1 aaaaaaaagg acagtttgat gagttggtaa gtaatttgaa tttgagcgaa caagaacaat
       61 tagacttgtt gcagcagtat aaagaggaac atcctcggcg agctaaaagg gggataaaat
      121 cagctataat aaaaaaagtc gctcgtttcc ttgctgctaa agtaggacaa aaaagtgtag
      181 tggagataac tgactattta tttgaatggc aagacaattt agaagctgga gcggagaatt
      241 atcttgtaca atatggttgg gatagaaata ttgctcactg gacaataaag acagttagct
      301 ttattttctt ataagtcatt atgaataaag cttggttatt ttggagtgtc atagtcttat
      361 atttttttat aaagtttttt gataaagttt tagatataaa gctttttggt tctattcaaa
      421 tatggctaga taatctccct attccgatga aaatactgtt aacaatagca ttagttttgt
      481 ttttattcat tgtatttcca tatagaggaa aacggtaaaa acggtaatta gatatataaa
      541 aggagctgaa agctccgtaa aaaacctact cgaagagtag gttttttctt atcttgatac
      601 tagtaggaaa taactctcat gctattttta gggtttcgac agggtctaaa cccttgatac
      661 tactgacttt ttaggtaaaa aaatgccctt gacttttttc aacggatctt ttataattta
      721 gttgttcaga aaaaattata aaaagagaaa aaaggcaaag gtatttttgt atttctttgt
      781 aattacaggt aaattataac atggagaagt gggaaaatca agataaaatt cttttagata
      841 aaaataaaag aggcaaggat agaaactggc gaggacgaaa attattaagt ttgaaattgg
      901 cagatatttt taaagagtta ggttataggg aaaccttgat agaacgcgtt gagacttgtg
      961 gagatactct tcgatttatt cgtcgagaag atgggagttt gcgtttatat caagcctatt
     1021 tttgtaagaa taaattgtgt cctatgtgta attggagacg gtcaatgaaa tattcttatc
     1081 aaacatctca gatagttgat gaagcaatta aagaacaacc aaaagggcgt tttcttttct
     1141 tgactttgac tgtgaaaaat gttccaggta aagaattgaa cgcgacaatt agccaactaa
     1201 ctcagtcctt tgaccgtctt tttagacggg caaaggtaaa gaaaaatcta atcgggtttt
     1261 tacgttcggt tgaggtgact cataatcaag aagaagagac ctatcaccct catatccatg
     1321 ttttgatgat ggttaagtct agctatttta gtggggctgg agataattat gtatctcaag
     1381 aagaatgggg gcggatgtgg gaacagtcgc ttaaggttga ctatgtgcca atagttgata
     1441 ttcggtcagt taaagaaata ggaaaaggtc ttaaaggggc aattcttgaa acggcaaaat
     1501 atccaattaa gccgattaag ttagatgttg aaaacaagca agttgttggt gatttgtata
     1561 atgggttata tcgtaaacgg caattgggtt acggtggatt gtttaaagaa attcggaaga
     1621 gattgcaact gtctaatgtc gaaaatggag atctggttta tatttctgat gacaatgatg
     1681 aaatgtcaaa aggtacaaaa atagtcgcga tttggaatgc gactaaacaa aactattttg
     1741 taaaaaataa gggttggaat taaagtatca actcttttat tataacataa aaaaagagta
     1801 aaatagactt gatttttttg agtttagatg tatattgaag gtgtgaaaat taaaaagata
     1861 gctatgtttt tttctgggta tacttgttgg ggtatttatt gttctatttt ttagaaatta
     1921 ttttgtttta cttttggaat atatttttgg ttaaaaagca ccctctaaaa agggtgcttt
     1981 ttgtgcggtt attcgcacga aagttctctg caagagttcc tcggaagagc gtttgtattt
     2041 tgattgtacg tcaaaactat aaaaacattt actctttgtc aaagagtagc agaaaagcta
     2101 gaataaaatt tttaatttta ttctagctaa tagtgcaagt aatagctttt gtagcttgcc
     2161 tcttgtccgt tattcagaca agaggcagga gtttgtattt gttctaaatg agtatttcat
     2221 ttagaacaaa tacatcgatg atgaacattg cttacaagaa tagagatttg agtgatgaag
     2281 aatttaaaga agaagctcat cagtttagag ataaagagat aaaaatactc gaagaaaaat
     2341 taaaggaact taatatttct tgtaaggttg ttggaataaa tagtataaaa gatatgaacg
     2401 agtataaaga gattatggaa aatgttaatc aagcaaaaga ggaattaaag agaatagatg
     2461 aattaatagc cgtcaggagc tccagaattg attttttaga ggataaaacc cttagaatta
     2521 caggaaacca aagatgagct taaaaacgaa atggagagcc taaaaagaga tttatctttt
     2581 tttagaaacg aaaatactcg actgaaaaat gtagtttcag attgtatcaa ggtagtattt
     2641 ttggggtatt tttagtagaa agtatattgt aatttctgag cgatttatgc cgtgaaagct
     2701 acttgaccgt aagctagatc aaagtacgct agtcagggac gagccgaaag gctgtagcgt
     2761 ttcggagcgg cacggacaaa gtacgtcgac ctaggttact tgcggtcaag tagaccatgg
     2821 aataaaacaa gcgggtatga tataaaaaag ttatcggcaa accgaaatat agacttgttt
     2881 ttttgtttat aaaaatgtat tattatattg agaataagat tgtttaattt atttagagag
     2941 gagtgtaagt tgatcagtaa acgattttaa ttctgccttt aaataacaag gagtttgtta
     3001 tgaataaaaa atttttgaaa tgtggtactt tatttttgat ttcttgttct attttaggaa
     3061 gtactattcc tgcagttaca gtattttctg atgaagtaac aattacttat aattcagaga
     3121 ataattcaga aaaaaatgaa ttatataatc aactttctgc tg
//
LOCUS       DAVMHG010000158         3140 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVMHG010000158 DAVMHG010000000
VERSION     DAVMHG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.160"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(313..846)
                     /locus_tag="VYR08_001910"
     CDS             complement(313..846)
                     /locus_tag="VYR08_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV3467387.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(935..1711)
                     /gene="recX"
                     /locus_tag="VYR08_001911"
     CDS             complement(935..1711)
                     /gene="recX"
                     /locus_tag="VYR08_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV3467388.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1749..3104
                     /gene="rlmD"
                     /locus_tag="VYR08_001912"
     CDS             1749..3104
                     /gene="rlmD"
                     /locus_tag="VYR08_001912"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3467389.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1004 a          549 c          565 g         1022 t
ORIGIN      
        1 aatgattgat gctatgtgga aatacaaaaa aatgtttttg atacgaagtt gacctgtatt
       61 ttttatacta atcattttcg tattttttgt attaaacgat ataagtttgt tgtaaactta
      121 ctaggaataa agacattaaa aaataatagt atatctattt gttttatata ttttacgaat
      181 tctgcataaa tctctttcta gtaatgtgtt gtaactctgc tataatggat ttattccttt
      241 ttgtgtttac acaatttatt ttatactacc aaaaaaacaa ggtcaggatt ttgttcctga
      301 cctttgacaa ctttaccgat tctttagttc tacatagcgc ttgtaccaaa tgtttacata
      361 ggcttctgag aaaggaccac gtccattgtt aatccaatca acaagaattt tgacatgttc
      421 ttttaaaata tagtccaagt catcagaata attcattttg cgtttgtgac gctcgtactc
      481 ttcaacgtcc aagagacgtt tttccccatc tgtaaaaatt ttaacatcca aatcgtaatc
      541 aatatacttc agtgcttctt catccagata gtaggggcta gccatattgc aatagtaaga
      601 agttccatta tcacgaatca tggcaatgat attaaaccaa tatttcttgt gaaagtaaac
      661 aatagccggt tctcgagtga cccaacgacg accatcactt tcggtaacaa gtgtatgatc
      721 gttgacacca ataatggcgt tttctgttgt ttttagtacc atggtgtccc gccaagttcg
      781 gtggagactc ccatcatgct tataactttg aattgtaata aagtcgcctt cttttggaag
      841 cttcataact aaccaacttt ctacaattta taagtttatc atttactatt gtaccataaa
      901 attacctaaa atctgtgaat ttcacttgga aatattaaag atattctcta agagcgcttg
      961 ctatatccga aaaatcgtag ccctttcgtg ctaaaacttg agttaaacgc tgcttcagtt
     1021 cgtatccttc atactttcga gcatacttag tatattgctt atcaagttcc ttgaagatga
     1081 gttcttgagt cgtttcttgg tcaacttggc tatctaagtc gtcaaaggca attttagcat
     1141 cagaataaga gaagcccttg ttagtcaagt tctggataat cttatcttgc agggcacgag
     1201 ctggaagttt tccctcatat tttttcaata gtttattggc tacacgttga gcaacttccg
     1261 aaaaatcaaa atcattcaag atttcttcta tagtagattt tgaaattcct ttttgtgcta
     1321 atttctgagt cagtacataa ggtcccttgt ctcctgaaag ttgattggca ttgatgatag
     1381 cataagcgta ctgaccatca ttaatccact tatcttcttt aagattagca ataacttgag
     1441 aaacgatgtt tttatcaata tcgtattttt tcagatattc tctgacttct ttttcagtgc
     1501 gtgctttaaa ggataagtgg tagagggcca gattcttacc ataagaaaat tgagcaaagt
     1561 cttgaatctc tttcaattcc tcttcgctta tcaccttatc tctcgataac ataaaacgaa
     1621 caattgtatc ttcggtgata tagcatttgt cgccattatc aagctccatc agatagagtc
     1681 tttttttctt ttcaagtttt gtgattttca tagctctatt ataactcaaa atgtgataag
     1741 ataggggtat gaatctgaaa gtgaaacaaa aaataccatt aaaaatcaag cgcatgggaa
     1801 ttaacggtga gggaatcggc ttttaccaaa aaacattagt ctttgtacca ggagctctca
     1861 aaggcgaaga tatctattgt cagattactt ctattagacg caactttgtt gaagcaaaat
     1921 tactgaaggt caacaagaag tctaaatttc gaattgtgcc atcttgtact atttataatg
     1981 aatgcggagg ctgccaaatc atgcacctgc attatgataa gcagctggag ttcaagacgg
     2041 acttacttca tcaagcgctg aaaaaatttg ctcctgcagg atatgaaaat tatgaaattc
     2101 gtccaactat tggaatgcag gaaccaaaat attacagagc taagttacaa tttcagaccc
     2161 gaaaatttaa aaatcaggtc aaggcgggct tatatgcaca aaactctcac tatttagtag
     2221 agttgaaaga ctgcctggta caagataagg aaacccaagt gattgctaat cgcttagcag
     2281 aattacttac ttatcaccag attccaatca cggatgagag aaaagttcta ggtgtccgta
     2341 ctattatggt ccgacgcgcg agaaagaccg gacaggttca gattattatt gttacaaacc
     2401 gccagcttaa tttaactcaa ttggtaaaag agttggttaa agatttccca gaagttgtga
     2461 cagtagctgt taatacaaat acagctaaaa ccagtgagat atatggtgaa aagacagaga
     2521 ttatctgggg gcaagagagt attcaagaag gtgtactcaa ttatgaattt tcactatccc
     2581 ctcgagcttt ttatcaacta aatcctgagc aaacagaagt cctctatagc gaagcagtaa
     2641 aagcgctgga tgttgataaa gaagaccatt tgattgacgc ttattgtgga gttggaacga
     2701 ttggatttgc ctttgcaaag aaagtaaaaa cactcagagg tatggatatt attccagaag
     2761 ctattgaaga tgccaagcga aatgctaaaa gaatgggatt tgacaatact cattatgaag
     2821 ctggaacggc agaagagatt attcctcgtt ggtacaagga aggctaccga gcagatgctt
     2881 tgattgttga cccaccacgt acaggtctgg atgataagtt attagatact attcttactt
     2941 atgtaccaga aaaaatggtt tatatttctt gtaatgtttc gaccttggct cgtgatttgg
     3001 tacgcttagt agaagtctat gatcttcatt atatccagtc ggtcgatatg ttcccacata
     3061 cagctcgaac tgaagctgtt gtaaaattaa taaaaaaagt ttaaaaaagg tgttgacaaa
     3121 acataaaaag gctgtataat
//
LOCUS       DAVMHG010000159         3107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVMHG010000159 DAVMHG010000000
VERSION     DAVMHG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.161"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            77..1423
                     /locus_tag="VYR08_001913"
     CDS             77..1423
                     /locus_tag="VYR08_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV3467390.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
     gene            1605..2828
                     /gene="dcm"
                     /locus_tag="VYR08_001914"
     CDS             1605..2828
                     /gene="dcm"
                     /locus_tag="VYR08_001914"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV3467391.1"
                     /translation="MIWVLDLFAGIGGFRMGMEAQGHECLGFCEIDKFARKSYKSIFQ
                     TEGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIA
                     RAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPKNR
                     ERVFIIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSRSGMSGKVYYSEGLAPT
                     LVRGKGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRYGIVVVGDLPTSF
                     KETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINL
                     ERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIR
                     RITPKECFRLHGFPD"
     gene            2856..2966
                     /locus_tag="VYR08_001915"
     CDS             2856..2966
                     /locus_tag="VYR08_001915"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV3467392.1"
                     /translation="MNSQLYKQVGNSVTVPVIAAIAKKLKEIEEKDKSIK"
BASE COUNT          988 a          568 c          667 g          884 t
ORIGIN      
        1 ttttttacta gacaaaaaag agtttcccct ttatggtata agtgtagaaa aaaacacaaa
       61 aagaaaggaa actcacatga acagtttacc aaatcatcac ttccaaaaca agtcttttta
      121 ccaactatct ttcgatggag gtcatttaac ccagtatggt ggtcttatct tttttcagga
      181 acttttttcc cagctgaaac taaaagagcg gatttctaag tatttagtaa cgaatgacca
      241 acgccgctac tgtcgttatt cggattcaga tatccttgtc cagttcctct ttcaactgtt
      301 aacaggttat ggaacggact atgcttgtaa agaattgtca gctgatgcct actttccaaa
      361 attattggaa ggagggcagc ttgcttcaca gccaacctta tcccgttttc tttccagaac
      421 tgacgaggaa acagtccata gtttgcgatg cctcaacctt gaattggtcg aattcttttt
      481 acagtttcac cagctaaacc aactcattgt agatatcgat tctacccatt tcacaactta
      541 tggcaagcaa gaaggtgttg cttataacgc ccactatcgt gctcatggct atcatcctct
      601 ttatgctttc gaggggaaga caggttattg tttcaatgcc cagcttcgtc ctggtaatcg
      661 ttattgttct gaagaggcag acagctttat cacacctgtt ttagaacggt ttaatcaact
      721 tctctttcga atggatagtg gctttgcgac cccaaaatta tacgatttaa ttgaaaaaac
      781 agggcaatac tacctcataa aactcaagaa aaatactgtt ctgagccgtc ttggagacct
      841 ttccctccct tgcccacagg atgaggactt aaccatcttg ccccactccg cctactcaga
      901 aactctctat caagcaggat cttggtcgca caagcgtcgt gtctgccagt tctctgaacg
      961 aaaagaagga aacttgttct acgatgttat ttctctcgtt acaaatatga cgagtggaac
     1021 aagccaagac cagtttcagc tttatcgtgg acgtggtcaa gccgagaatt tcatcaagga
     1081 gatgaaggag ggattttttg gcgataaaac ggatagttca accttaatca aaaacgaagt
     1141 tcgtatgatg atgagctgta tcgcctacaa tctctatctt tttctcaaac atctagctgg
     1201 aggtgacttc caaactttaa caatcaaacg cttccgccat ctttttcttc acgtggtagg
     1261 aaaatgtgtt cgaacaggac gcaagcagct cctcaaattg tctagtctct atgcctattc
     1321 cgaattgttt tcagcacttt attctaggat tagaaaagtc aacctgaatc ttcctgttcc
     1381 ttatgaacca cctagaagaa aagcgtcgtt aatgatgcat taaagaacag tcgagatgaa
     1441 aaaatcgtgt gacgaaccaa gggaggagtc tgcccttttg aggaaatcta gcgaggaaaa
     1501 acgatactgg aacagcagaa agtaaaactg acctcatgag gaggaagaaa gtggctcatg
     1561 aggtcagggg ttttgtaagt tacatctagt tgagagaggt atgaatgatt tgggttttag
     1621 atttatttgc tgggatagga ggctttagga tgggaatgga agctcaaggg catgagtgct
     1681 tgggattttg tgagattgat aaatttgcta ggaaatctta taagtccatt tttcaaacgg
     1741 aaggagaaat agaatttcat gacatacgag atgtttcaga tgacgaattt aaaaaactta
     1801 gagggaaagt cgatgtcatc tgtgggggat tcccttgtca agcattttca atcgcaggaa
     1861 gacgattggg atttgaagat actagaggaa ctttgttctt tgaaattgct cgggcggcca
     1921 aacaaatcca accacgtttt ctttttcttg aaaatgttaa aggcctactc aatcacgata
     1981 agggacggac gttcaccaca atacttacca cacttgatga gttggggttt gatgttgagt
     2041 ggcaggtgct taacagtaag gattttggcg ttcccaaaaa cagagagagg gtgtttatta
     2101 tcggacattc tagaaagaaa ggtaccagac tcttatttcc tttcagacga gaaggtcaag
     2161 caactaactc tgagactttg aaagcactag gaaatttgaa tccatcaaga agtggaatga
     2221 gcggtaaagt ctattattca gaaggtcttg cgccaacctt agttcgtggt aaaggagaag
     2281 gatttaaagt tgcgattcct tgtatgacac cagatagatt agacaagaga caaaatggga
     2341 gacgctttaa ggaaaatcaa gagccgatgt ttactttaaa tactcaggat cgttatggca
     2401 ttgtcgttgt tggagattta ccgactagct ttaaggagac aggtcgtgtc tatggaagtg
     2461 agggcttatc tccaacactg actacgatgc aaggaggaga taaaatcccc aaaatactaa
     2521 ttccagaacc aatccaattt ctaaaagtac gggaagcaac gaaaaaagga tatgctcaag
     2581 cagagattgg ggattcaatc aatttagaaa gaccaagttc tcagtatcgt cgtggtagag
     2641 ttggaaaagg tatagctaat acgttaacaa ctagtggtca aatgggagta gtagtggcta
     2701 gctatgaagg agaagataaa caagtttacc aagtagctgg tgttttaata gatggacaat
     2761 tttaccgttt gagaatacga cgaatcactc ctaaagagtg ttttcgattg catggctttc
     2821 ctgattaggc ttttgaagct gctagaaaag tctctattaa tagtcagctc tataaacaag
     2881 ttggtaatag tgtaaccgtt cctgtgattg ccgcaatcgc aaagaaatta aaagaaatag
     2941 aggaaaaaga taaaagcatt aaataaagaa tcaatactag attgtgatga attagaaaca
     3001 gaattacatg atgcagaaat caaacagctg gatgaacaat tatttttgat gcccaattat
     3061 ccatgtgagt ttgaagtaac atttttagat gattaccata aaaaaca
//
LOCUS       DAVMHG010000160         3097 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVMHG010000160 DAVMHG010000000
VERSION     DAVMHG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.162"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(153..1076)
                     /locus_tag="VYR08_001916"
     CDS             complement(153..1076)
                     /locus_tag="VYR08_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV3467393.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(1090..2019)
                     /locus_tag="VYR08_001917"
     CDS             complement(1090..2019)
                     /locus_tag="VYR08_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3467394.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(2338..2559)
                     /locus_tag="VYR08_001918"
     CDS             complement(2338..2559)
                     /locus_tag="VYR08_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467395.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSRKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(2513..2773)
                     /locus_tag="VYR08_001919"
     CDS             complement(2513..2773)
                     /locus_tag="VYR08_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467396.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT          993 a          603 c          608 g          893 t
ORIGIN      
        1 gcactgcttt gaggttgtag ataagactga cgaagtcagt tacatatatc tacggcaagg
       61 cgacgttgac gcggtttgaa tttgattttc gaagagtatt aacttcacac aagggaagtt
      121 gggaactgag aaatgttatt tctcaataag cactattctt tcacaccacc gatagtcaaa
      181 ccttttacaa agtagcgttg gaagaatgga tacaaaatcg cgattggaag ggttgcaacc
      241 acaaccatgg ccatacgacc tgtttctttc ggtagagcaa ctcccagttg accagtcaag
      301 ccgaccgctt tggcaatgta gtccatattt tgttggattt gcatgagcaa atattgcaat
      361 ggatacaagt tgtcactctt gatgtaaaga agggcgttga accagtcatt ccagaaacca
      421 agagctgtca aaagcgtgat ggttgcgata cctggtagtg acaatggcaa acagatttgg
      481 aagaaaatcc gggcctcact ggcaccatcg atacgagccg attcgagaat ggcttctgga
      541 atggtcttct tgaagaagga acgcatcaag atgatattga atggtgagag aagcattgga
      601 acaatcaagg cccaaacagt gtcaccaagc tgaagtacac gggtcaccat gatataacct
      661 ggtaccaaac cagcgttgaa caacatactg agaaggacga agatggtaaa aaatctgcga
      721 tacttaaagg ttgtccgtga aatagcgtag gcataggttg ttgtgataaa gacatttgtc
      781 aatgtcccaa ctacggttac aaagacagag atgaagaggg cttgtaggat tttatcctta
      841 aactgtgcca aaaactcaaa accgtctaag ccaaattggg atgggaagaa gctatagccg
      901 tattggagga ggcttttctc gtctgtcact gaaataatga taacgaatac aaaaggtagg
      961 atacaagaga gggcaatcaa acccgaaatg atactgaaga agatatctgc tttcttactg
     1021 aaggagtgaa tgccgacatt atcaattttt tcttttttaa ttttcttttt tgccatattc
     1081 tcctcctttc tagaacaaag ctgagtttgg atcgactcgt cttgcaagca agtttgatag
     1141 gataaccaga atcaaaccaa caacggattg gtaaagaccg gctgctgcag ccataccgat
     1201 atctgctgtc tgagtcaaac cattaaaaac atatacgtcc aaaacgttgg ttacattgta
     1261 aagctgacca gcattgtgtg ggatttgata gaagagaccg aagtctgcgc ggaagatatt
     1321 tccgactgca aggatggtca atacagttac aagcggagtc aactgaggaa tggttacgtt
     1381 gcgaatacgt tgccacttgc tagctccgtc cactgtcgct gcttcgtagt aggttggatc
     1441 aattcccatg atcgtcgcat agtacatgac actgctatat ccaaagcctt tccaaatacc
     1501 taggaaaagt aggagatagg gccagatgcc caggtcagcg tagaaattga cttctttgag
     1561 accaagactt tccaatagat gattgaacac ccctttatca atatttagga aggcatctgt
     1621 aaagaaacta atgataaccc aagacaagaa gtaagggaac aacatagaag tttgaaaaat
     1681 cttcaccatt ctcttagaac ggagctcgct gaggataatg gcaatcccta cagatacaac
     1741 taaacctaga aagataaagc caagattgta gaggacagta tttcgtgtga taataaaggc
     1801 gtctcttgaa ctaaataaga atctgaaatt atcgagtccg acccatttac tatttatgat
     1861 actatctatg aaaccattac tggtcatgtg gtagtctttg aaggcaacca cgttcccaaa
     1921 tactggaatg taaaagaata gaatcaacca gagtgcccct ggcaaaacca tcaagagaaa
     1981 gatccagttg tctctcaatg tttttgaaaa ctttttcata atttcctccc tttttatttt
     2041 gatatccatc taaaaattct tttttagact tttgataacg attacattat tagtatactc
     2101 ctatttgcag gttaggttaa actcctaatt atagaaaaaa ctccacaaat tatgtagcag
     2161 atttaaaact ttatcaccac tatcaaacaa atgtcctaaa tcaattgttt attttatctc
     2221 tattagccca gtgatggcgt cactctgtta taagcatcca acaacggggt atactgaaaa
     2281 atctccagac tagggaactc agagatagtt cctaatctgg agatttttaa tatgttatta
     2341 ggcgtttgct ttcaacttag caataacctc tttaagatta tcaatcacct ctgcttcagt
     2401 atgctcagag cctttttcat ctgccaagaa caaaactgct ttttgaagtt cttttcgaga
     2461 gttttcaagg acatccttat ctactgtttc aaggtttgag tctttaagaa gtttacttaa
     2521 ttccttggct aatttcttga gtttgatttg cagactcatc ttctcctgct gtttctttgc
     2581 ccgctgtttg tcctccatcc ttagttgctg actggctttc cttaatggac tctagggaag
     2641 caatggcatc tttgactgtt tgcaagatat cacgtaaacc ttgctctgtc aaactatcat
     2701 ctgcaagagc tttcttagcc tctgccaaaa ccagacgtgc tgaatctgtg gtaggattcg
     2761 atacacctgt caatgatctc aaaagatttt ctaaggtttg agtctgctta ctaatactag
     2821 actaaaatca aaaagcatta cataacagtg atatgaaatc aactaaagaa gaaatccaaa
     2881 tcatcaaaac acttttaaaa gactctcgta cagctaaata tcataaacgc cttcaaatcg
     2941 ttctatagta aaatgaaaca agaacaggac aaatcgatca ggacagtcaa atcgatttct
     3001 aacaatgttt tagaagtaga tgtgtactat tctagtttca atccattata tttcgtctga
     3061 tgggcaaatc ttaataaaga gattatagag cttttat
//
LOCUS       DAVMHG010000161         2926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVMHG010000161 DAVMHG010000000
VERSION     DAVMHG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.163"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(48..1004)
                     /gene="lytA"
                     /locus_tag="VYR08_001920"
     CDS             complement(48..1004)
                     /gene="lytA"
                     /locus_tag="VYR08_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV3467397.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1380..2750)
                     /locus_tag="VYR08_001921"
     CDS             complement(1380..2750)
                     /locus_tag="VYR08_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV3467398.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIAQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT          920 a          644 c          565 g          797 t
ORIGIN      
        1 caagacctaa taatatgcgc tgttctgatt tgaaagacat tccattatta ttttactgta
       61 atcaagccat ctggctctac tgtgaattct ggcttgtctg ccagtgttcc gtctggtttg
      121 aggtagtacc agcctgttcc gtccgctgac tggataaagg catttgatac catggcgcct
      181 tctttagcgt ctaagtagta ccaagtgtcc ttgtacttga cccagcctgt cttcatggca
      241 ccttcttcgt tgaaatagta ccacttatca gcgattttct tccagcctgt agccatttcg
      301 cctgagttgt cgaaccagta ccagttgccg tctgtgtgct tcctccagcg gtctgcaagc
      361 atatagcctg aactgtcaaa gtagtaccaa gtgccattga ttttctcaaa cttgtctttt
      421 ggataagagc cgtctgaatg tacgtaccag tagccagtgt cattcttctg ccagcctgtt
      481 tcaatcgtca agccgttctc aatatcatac ttaaactgct cacggctaat gccccatttt
      541 gccaagtaag ggtatggatc cacatggtct gagtggttgt ttggttggtt attcgtgcaa
      601 tactcgtgcg ttttaattcc agctaaactc cctgtatcaa gcgttttcgg caaacctgct
      661 tcatctgcta gattgcgtaa gagttcgata taaaggcggt agtccgtcat gaactcttct
      721 ttagttgaat ggctttcaat cagttcaacc gctgcatagg tctcagcatt ccaaccgccc
      781 ccaacgtccc aggcaccatt atcaacaggt cctacctgca tgatgcaacc gttcccaaca
      841 atgtgcgaga aaaaacctaa ttctgggtct ttccgccagt gataatccgc ttcattctgt
      901 acggttgaat gcggattccc agttgagtgt gcgtgtactt gcctatatgg ttgcacgccg
      961 acttgaggca aatctgttct taatttactc acattaattt ccatattcta ctccttatca
     1021 attaaaacaa ctcatttttt acaatccaga accagaaact cctttatttc taccttacaa
     1081 agaagacaat cttagtcacg attaggcttg tagatagaac ctcaaaacgc actattttga
     1141 cactgtaaat aggactgaca aggtctgcat tctatctaca ataacacccc agactaaaaa
     1201 gcttttcaaa agtatatttt tacagtctct atatgtcctt ttcataaata ctatacttta
     1261 ttatatcata taaaagaagt caaaagtctg ttaaactatt ttcaacacca aactaaagaa
     1321 gagaacacaa gttttttcga tgttcactag aggaaatgga ttttattcag taaatccaac
     1381 taggattgca ctttggttgc caagattgcc tttccttctt ttatcaaggg atgacggaat
     1441 agtgagaagt acagttgaac tgtcatggca acccagataa gaggttcaca aaggataaca
     1501 cccttatatc ctgcccaagg aataatcaaa accacaaaaa cgatttttcc gattagttca
     1561 ataaagctag aaactagagg aaggatcttc tgccccaagc cctgcaagca attgcgataa
     1621 atcaacaaga ggctcaaaat gggataaaag gctgaactga tttgcagata gagacttcca
     1681 ttttctatca agtaaccatc tgtcgaacta gccaagaagg aaaccaaggc tggactggca
     1741 aaaaagagga aaatacaaac aaaaactgcc caggatatac ttaaacgact gccgattcga
     1801 agaccttgag caatgcggtc aggtcgctta gctcctagac tctgagaagc aaaggtcgtc
     1861 attgatgcag aaatagcggt cataggaaga agggcaaagg tcataatgcg tcgagctgcc
     1921 gtctgagcac taataatcac tgcaccaaat gtattaacag aagactgtaa aatcacactg
     1981 ccgatagata caattgaact catcaagccc atagccaaac cctgctccaa gagatccgcg
     2041 tacaagcttt tgtcccattt gaaatgttta aactgtggca agagttctgg cacactttta
     2101 cgaatataat aaaagcagag aaccgctgat aaaccttgcg aaataatggt agcaagtcct
     2161 gcggattgaa ctcccagatg caattgcgta ataaaataga gatccagaac cacattaacc
     2221 aaggcagaga aaatcagaaa tcccagggct gctagactgt caccaataga ccgcaacaag
     2281 cctgcaaaaa gattataagc aaagctgaca cctacacagg tcacaatcat agaaatatat
     2341 tgataagatt gaggaagaat ttctgcagga gtatctaagt attgcaagag aggatacaag
     2401 ccaagaaagc ccagcaacat aactagaatg ctcaaaagag cacctaaaat ccaggtggct
     2461 gctactgctt ccttgatttt agtgaaattc cgagccccat aataacgagc aatgacaatc
     2521 cccatgccat tgccaacacc aagtgtaaaa cctacaatca ggtcaaaaat cgctgtcgtc
     2581 gctcctactg cagccaagga ttcttgacca agaaatcgtc caacaatcaa gacatcagca
     2641 gtgttataga gctgttgaaa aatatttgat agcaagattg ggaaggcgaa gcttaagagc
     2701 gagagaagaa tcggaccatg tatcaggtct actgttcgtt ttttattcat aaagctatta
     2761 tatcagcttt ccagaggagc gacaagaaac agcaaactat atatagcgga atgaaataag
     2821 gacagtcaaa tcgattttta acaatgtttt agaagtagat gtgtactatt ctagtttcga
     2881 tctattataa ttggttgagt ttggaatagt acgctgtagc tgctaa
//
LOCUS       DAVMHG010000162         2923 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVMHG010000162 DAVMHG010000000
VERSION     DAVMHG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.164"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(162..623)
                     /locus_tag="VYR08_001922"
     CDS             complement(162..623)
                     /locus_tag="VYR08_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467399.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     DRYLYRPYAGAPRGEKVYGKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(590..691)
                     /locus_tag="VYR08_001923"
     CDS             complement(590..691)
                     /locus_tag="VYR08_001923"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467400.1"
                     /translation="MKGGHRHVEAAKVFDVGVRTLFTWEKKDVNKGT"
     gene            1002..1382
                     /locus_tag="VYR08_001924"
     CDS             1002..1382
                     /locus_tag="VYR08_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV3467401.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1481..1702
                     /locus_tag="VYR08_001925"
     CDS             1481..1702
                     /locus_tag="VYR08_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV3467402.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1719..2033
                     /gene="trxA"
                     /locus_tag="VYR08_001926"
     CDS             1719..2033
                     /gene="trxA"
                     /locus_tag="VYR08_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV3467403.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2338..2871
                     /gene="queF"
                     /locus_tag="VYR08_001927"
     CDS             2338..2871
                     /gene="queF"
                     /locus_tag="VYR08_001927"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV3467404.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          926 a          603 c          533 g          861 t
ORIGIN      
        1 atagcaagaa acttgaggta tttttgctaa aaaaaaaagc attttactat tttgtatata
       61 tatatatata ttataagata agttttttat aaagacacgc aaatgagtat aaatcagcta
      121 atttttggtt tatattccta tttttatgca taattattct ttcattctct aatttgtgaa
      181 tcaatatatc tgattagaaa actaccattg agtttttgct ccacctcatt agtccgctca
      241 aaacgccgtc cgctaatctt gccatagact ttctcccctc taggagcccc tgcataagga
      301 cgatagaggt agcggtcgat tcccgtttcg tcaatatata ggactggtaa atcttttagg
      361 ttatccaaaa tatcaagaaa ctcagaaact ttctcaggct tttgttccct aaaactggtc
      421 gtctttttta aaatgacctt aatctgcttt aaaactgccc atacggaggg caaagcacaa
      481 tcaaaacggg ccgcaatttc ccgtaaaaaa gcgtctggat gagcctctac aaaggctttc
      541 aattcttcta aagggatctt acgctttttg acgactcgct ttttccgctc taagttccct
      601 tgtttacgtc tttcttttcc cacgtgaaga gagttctgac gccgacatca aaaactttgg
      661 ccgcctcgac atgtctgtgc cctcctttaa tggaatctaa tgctccttgt ttaaaatcag
      721 tactatatgc atagctttat tagaccatgc ttattttaaa ctaagcaact atagatagag
      781 aaatgaaata aaatctgaat tcgaatgtga taaggagtta gaaattgaaa caataggatt
      841 cccccattat tcacacaaaa ataaaaccac taaaaaacag agcttgccag cattcttgaa
      901 aagtagtata attattgcat gcgcaatcat cttatgacgc aaagactgcc cgttaatgga
      961 ctttcttcta taattatgac tctaaaaaga aaggagacac tatgacctat ttggaaaaat
     1021 ggtttgactt caatcggcgt cagaaagaaa ttgaaagtct cttggaagag accattgccc
     1081 aacagagcga gcaaagtctg accttgaaag agttctacct gctctactat ctggacttgg
     1141 ctgaagaaaa atctctacgc cagattgatc tgccagataa gcttcatctg agtccaagcg
     1201 cagtttctag gatggtggct cgcttggaag ctaaaaattg cggtctactc agtcgcatgt
     1261 gttgccatca agatagacgc tctagtttta tctgcctgac aaatgatggg caaaagacac
     1321 tggcctcgct acaaaagact gtcgaagaaa gcttggaagc aggtttggat ttcttgattt
     1381 aagatgattt taaaaaaagt tgcgtgcgca atcatttttc ttgacattct cttttataag
     1441 gagtaaaata aagttagcat taaacaaagg agttttaaac atgattgaaa ttacctatat
     1501 agatgccagc aagaacgaaa gaactgtgac tttcgagtct tatgaagact ttgaacgttc
     1561 gcaacaagct tgccttatcg gcgtcgcaga ctactaccct gtccaaaaat taacttacaa
     1621 gggtcataat ttggactacc atgggactta tggagatatc ttcttctatc tcatgaaaca
     1681 agatttaagc caatataact aaaaaaagga gaaatacaat ggcaaaagca attacagatg
     1741 caacattcga acaagaaaca aaagacggtt tggtcttagt agacttctgg gcaacttggt
     1801 gtggtccatg tcgtatgcaa ggtccaatct tggacaaatt gtctgaagaa ctttcagaag
     1861 atgtcttgaa aatcgttaaa atggacgttg atgaaaatcc aaatacagct cgtgcttttg
     1921 gaatcatgtc tattccaact cttctcttca aaaaagacgg ccaagttgtc aaacaagttg
     1981 caggtgttca cacagcagaa caaatcaagg ccatcattgc tgaattgagc taatcaaacg
     2041 agagaccaag tactttcttt ggtctctttt ttcttgccct ttgccatttt tcaaaaaata
     2101 tgctagacta tagtagattg aaactagaat agtacacctc tacttctaaa acattgttag
     2161 aaatcgattt aactgtcctg atcgatttgt cctgttctta tttcatttta ctatataagt
     2221 agaatattac gacgtttggg atatgccatc gctaaaaaaa ctcgatttgg tattttttag
     2281 ctccctaatg ggagcttttt gcgtgctctg aacatttccc attttggaag gagtactatg
     2341 aaacatcaat cagccttggt cgtctttagt ggtggtcaag attcaacaac ctgccttttc
     2401 tgggccatgc aacactatga aacagtcgaa gctgtcacct ttgcctacgg ccaacgtcat
     2461 cacctcgaaa ttcaaattac tagagaaatc gctaaggaac agggcattcg tcaccatatc
     2521 ctcgatatgt ctctgctggg acaaatcact gctcagccag actttgcgac gattcatatt
     2581 tcctacattc ctgacaagct ctgtgtcgag tcaaaatccc tcaaactata tctatttagc
     2641 taccgaaacc acggagattt ccacgaaaac tgtatcaaca ccatcgggaa agacttggtc
     2701 aacttgctag accctcgcta tttagaagtc tggggaaaat tcactccgcg cggtggcatt
     2761 tcaatcgacc cctactacaa ctacggtaag caaggaacta agtatgaggg cttggcagaa
     2821 caacgcctct tccaacacga cctttatcca gagaaaattg acaaccgcta aagtcatact
     2881 caatgaaaat caaagagcaa actaggaagt tagccgcagg ttg
//
LOCUS       DAVMHG010000163         2905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVMHG010000163 DAVMHG010000000
VERSION     DAVMHG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.165"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(41..952)
                     /locus_tag="VYR08_001928"
     CDS             complement(41..952)
                     /locus_tag="VYR08_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV3467405.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(976..1779)
                     /locus_tag="VYR08_001929"
     CDS             complement(976..1779)
                     /locus_tag="VYR08_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV3467406.1"
                     /translation="MSIISMVLVVGVAFLAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPATLL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(1807..2796)
                     /gene="manX"
                     /locus_tag="VYR08_001930"
     CDS             complement(1807..2796)
                     /gene="manX"
                     /locus_tag="VYR08_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV3467407.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVANAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT          877 a          625 c          605 g          798 t
ORIGIN      
        1 gaatcagatt ttagaacctg gttccttttt agttgcttga ttacatgatg tgaagaacat
       61 gtgccacaat acccactgcg aagagagcaa ggataatagt gattggagat acttttttct
      121 taagtaagta catgcaaagt aaagtaagga gtagtcctga taatccagga atcaacatat
      181 ccaagttttg ttggaaagta gtaacttttt caggagtttg agacaatcct tgtcctactt
      241 gtgcgaatgc ttcttggata cctttagacc cttctggcaa tttatcccaa tggatataag
      301 ccttttcatc tagttgaact ttagaaacat cgaaagcaaa tttaatattt acccagcgtt
      361 gaacaaggac agcaagaatg aacatcccaa ggatagaagc tcctttagtg atatcttgaa
      421 ggataccacc agacatatct ttagtgattt ctgatccagc cttgtatcca atctcttgaa
      481 cataccacaa gaatgacata cgaatcaagt tccatgcaac aaagaagagg agtggcccca
      541 agatattgcc agtaagggca agtgaagcac cgagagatcc aaggattggg cgtactgtaa
      601 accagaatac tgggtcaccg ataccagcaa gaggtcccat cataccgatt ttaacccctt
      661 ggatagcagc gtcatcgatt tccacaccgt tagcacgttc ttcttcaagc gcaagagtaa
      721 cccccatgac tggagcagct acgtatggat gagtgttgaa gaactcaagg tgacgctcaa
      781 gagcagcgat ttgatcttct tttttagtat agagtttttt gatagctgga atgagtgtat
      841 aagcccagcc caagttttgc atccgttcaa agttccaaga cccttgtaag aaggttgaac
      901 gccaccaaac ttttttacga tctgatttag ttagttgaag tttttcagtc atgatgtttt
      961 cagtcctttc ttatcttagt agtcttctag gatatcgccg attgggtcgt tagaagttgc
     1021 ggctcctccg ccaccatttc caccagtttt agaaagatga aggtagataa gagcgatagc
     1081 aacaccgata gcaccgaatc cgattagagt aatatctgac acagcagcga gaacgaaacc
     1141 aagagcgaag aatggccata cttcacgagt tgccatcatg ttgataacca tggcgtaacc
     1201 aacggcaacg accataccac caccgatagc cataccatct ttgagccagt ctggcatggc
     1261 actaaggata ctttgtacag tttcagttgg taccataagg agaagagttg caggaagcgc
     1321 gatacgaagt ccttggaaaa gtagcgcaat aaaatgcgca cgctccacag cgccgaagtc
     1381 acctttttta gcggcagcat cagcagtgtg aaccaatcca actgaaattg tacgtacaat
     1441 catagttaag aatagaccag caacggcaag agggatagca accgcttggg caacaccgat
     1501 acctgtcttg gtaaagtcac caccaagaac catgataatg gcagcagcga cagaagcaag
     1561 tgcagcatca ggagcgatag cagcaccgat atttgaccaa ccaagggcaa tcatttgaag
     1621 cgatccaccg aggataatcc ctgcttccaa gtgacctgtt acaagcccaa taagggtaca
     1681 ggctacaagt ggttggtgaa attggaactg gtcgaggatg ccttcaagac ctgcaaggaa
     1741 ggctacaccg actactaaaa ccatagaaat aatagacata tttaaaatcc tttcataaat
     1801 aatggcttat ttgacattgg ctttgttaat caagtcaaac aaatcttttt tagaatcatt
     1861 tggtacttta cggacatcaa attcaacacc caagtcacgc attttttcaa atgtagcaac
     1921 gtcttctttg tccatagaca aaacggtatt gaccaatgtt ttacctgttg agtgagccat
     1981 agaaccaacg ttaagagtct tgattggcac gccgccttcg atggcacgaa gggcatcttg
     2041 aggtgtttca aacaagataa gggcatgtgt ttctccaaaa cgtgggtctt ttgaaatctc
     2101 aatcagtttt tgaattggaa ccacgttagc cttgacatta cctggagctg cttgtttaat
     2161 caattcttta cgaaggtcgt ctttagccac gttatctgaa gcaacgatga tacgatttgc
     2221 ttttgaatct ggagtccaag cagttgcaac ctgaccgtga agtagacgtg tgtcaagacg
     2281 ggcaagattg attttcaatt taccgtctcc gataacagtt ccttctggga tagcagtttg
     2341 ggcaactgga gcagctgcag cgtttgcaac ttcttcaact ggatttagct cttctggaag
     2401 agctttgatg ccatctttgg cttctttaat gatattagca gcgacttttt ctacacctgc
     2461 agcagcgtcc atgaggcgct ctgtgtaggc ttgaatcaac atcggtaagt taagtcctgt
     2521 gatgatggca aacttacgct caggattttc tcccatcacg cgactagctt ggttaaatgg
     2581 agaaccactc caaaggtcag ccaaaactag aacctcatct tctgcgtcaa atgcagcaac
     2641 agcgttatta aacttagcgt atagatcatc aggaccttca tttggcataa aggtcacaac
     2701 ttgaaccttt tcttgttcac caaagatcat agatcctgac tgatgaatac ccgcagcaaa
     2761 ttcgccgtgg ctcgcaataa tgattccgat actcattatt gtcattcctc cttttttgtt
     2821 ttagttttca tttaagaaaa ctttaagact gtttaagtat aaaccgtttt cataaaaaaa
     2881 gcaactattt atcataaaaa attaa
//
LOCUS       DAVMHG010000164         2891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVMHG010000164 DAVMHG010000000
VERSION     DAVMHG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.166"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            5..763
                     /locus_tag="VYR08_001931"
     CDS             5..763
                     /locus_tag="VYR08_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV3467408.1"
                     /translation="MTSDVYSKIMNVANDSSLQLYSSAKIQKLGDKSPLEIKIETPEN
                     ITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSLEANGAKVPQANKDL
                     AQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESKGNLTEEDVRKIVED
                     TLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDLKQSIVSQAGDSFKN
                     INLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            837..2468
                     /gene="rlmD"
                     /locus_tag="VYR08_001932"
     CDS             837..2468
                     /gene="rlmD"
                     /locus_tag="VYR08_001932"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV3467409.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLIRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSQKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            2622..2771
                     /locus_tag="VYR08_001933"
                     /pseudo
     CDS             2622..2771
                     /locus_tag="VYR08_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT          961 a          522 c          612 g          796 t
ORIGIN      
        1 gaccatgaca tctgatgttt attctaaaat catgaatgtg gccaatgact ctagcttaca
       61 gttgtattca tcagccaaga ttcaaaagct aggtgacaaa tcgccacttg agatcaagat
      121 tgaaacacct gaaaacatca ctaaggtaac tcaggatatg taccgaaatg cagccgtaac
      181 tttgggtgtg gaacacgcca aaatcactgt agcagcccct attccggtta caggagaaag
      241 tgccttagca gggatttact attcgctaga ggctaatgga gccaaggtgc cacaagctaa
      301 taaagatttg gctcaagaag agttgaaggc tttgtcagat atcaatgctg aaaacaagga
      361 caaatcaggc tatgatgcta ataaattaaa cgttgcccta gctgatatca agtcaggact
      421 tgccaaagct aaagaaagca agggaaatct gacagaagaa gatgtccgca agattgttga
      481 agatacctta aaaaattaca aacttgatca ggtcataaca ggaaaccaga tcaatatcat
      541 catcaatttt gccttgaatc tctcaaagag tgatatcctc agcaatgcag atttcactaa
      601 aaccctaaat gaccttaaac aaagcatcgt atcacaagct ggcgacagtt ttaaaaatat
      661 caaccttaac tttgatgcgg ataaggcgct agaggacggt ggtaacttct taagctccct
      721 ctggcaagcc cttgtcaact tcttcaagag ttttggttct taagaaattt catggtataa
      781 tggatggtaa ccgtaacgtt gccgtctttt ttgtcggcca atagaaagag aaaagaatgt
      841 taaagaaaaa tgatattgta gaagttgaaa ttgttgattt gacccatgaa ggggcaggag
      901 ttgccaaggt agatggtttg gtcttttttg tagagaatgc tttaccgagt gaaaaaattc
      961 tcatgcgtgt cctcaaggtc aataaaaaga ttggctttgg aaaagttgaa aaataccttg
     1021 tccagtcacc acaccgtaat caagatctag atttggctta cctgcgttca ggaatcgcgg
     1081 atttaggaca cctttcttat ccagaacagc tcaagtttaa aaccaagcaa gtcaaggaca
     1141 gtctctacaa gattgctgga attgcagatg tagaagttgc tgaaacgctt ggtatggaac
     1201 atccagtcaa gtatcgcaat aaggcgcagg tgcccgttcg tcgagtgaat ggtgtcttgg
     1261 aaacaggatt tttccgtaag aattcgcacg acctcatgcc ccttgaagat ttctttatcc
     1321 aggatcctgt cattgaccaa gtcgtagtag ctcttcgaga cctgatccgt cgttttgatt
     1381 taaaacctta tgacgaaaag gaacagtctg gattaattcg gaatcttgtg gtgcgtcgtg
     1441 gccactattc agggcaaatc atggtcgttt tggtgacaac tcgtccaaaa gtttttcgtg
     1501 ttgaccaatt gattgaacaa gttatcaagc agttcccaga gattgtgtct gtcatgcaaa
     1561 atatcaacga ccagaatacc aatgcgattt ttggtaagga gtggcgcact ctttatggtc
     1621 aagactatat tacggaccag atgttgggaa atgacttcca aatcgctggc ccagcctttt
     1681 accaagtcaa tactgaaatg gcggagaaac tctatcaaac agccattgac tttgcagagt
     1741 taaaaaaaga tgatgtgatt attgatgcct attctggtat tggaaccatt ggtttatcag
     1801 tcgccaagca tgtcaaagaa gtctacggtg ttgaactgat tccagaagca gtagagaata
     1861 gccagaagaa tgcttctttg aacaagatta ctaatgccca ctatgtctgt gacacggctg
     1921 aaaatgccat gaagaaatgg ctcaaggaag gtattcaacc aaccgttatc ttggttgatc
     1981 ctccacgcaa gggcttgaca gaaagcttta tcaaagcaag cgcccaaaca ggagccgatc
     2041 gcatcgccta tatctcctgc aatgtcgcaa ccatggcgcg tgatattaaa ctataccaag
     2101 agttgggata tgaattgaag aaagtccagc cggtggatct atttcctcaa acgcatcacg
     2161 tcgagacggt agcacttttg tccaaactcg atgtcgataa gcacataagt gttgaaattg
     2221 agctggatga gatggatttg acaagtgcgg agagcaaagc aacatatgct caaatcaaag
     2281 aatatgtttg gaataaattt gaattaaaag tttcgacatt atatattgca cagataaaaa
     2341 agaaatgtgg aatagaatta cgagaacatt acaacaagtc tcaaaaggat aaacaaatta
     2401 ttccacagtg tacacctgaa aaagaagaag ccatcatgga tgctttgaga cacttcaaaa
     2461 tgatttaata gaaaagaatg acagtatatg actttctgca tttattacat tcctacttgg
     2521 tatagggaca gctattattc ctttcttgca aggtatcaat tagaaaatag gctcaatata
     2581 aagattgata ggatcatttt tatatttaaa ggagcgctga aatgattgat aaaggcaaca
     2641 aaaaatttta ggataaattt gctaagttgt atgccccttt tatgaaaaaa gataaagagg
     2701 tttatgataa agtttgtgaa tatcttagtc ctcatttgaa taaagatatg gaggtgcttg
     2761 aacttgcttg ttggtttcgt gtcataatag ttatagaggc aaatagttat gtaaatataa
     2821 ggagttcaag acttctacca aagtttaaaa ctcaaaaaat aaatagttgg tgtgctgctt
     2881 acaatatcca t
//
LOCUS       DAVMHG010000165         2871 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVMHG010000165 DAVMHG010000000
VERSION     DAVMHG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.167"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            14..568
                     /locus_tag="VYR08_001934"
                     /pseudo
     CDS             14..568
                     /locus_tag="VYR08_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            571..750
                     /locus_tag="VYR08_001935"
                     /pseudo
     CDS             571..750
                     /locus_tag="VYR08_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            843..1637
                     /locus_tag="VYR08_001936"
                     /note="aph(3')-IIIa"
     CDS             843..1637
                     /locus_tag="VYR08_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEV3467410.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1913..2486
                     /locus_tag="VYR08_001937"
                     /pseudo
     CDS             1913..2486
                     /locus_tag="VYR08_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            2815..>2871
                     /locus_tag="VYR08_001938"
     CDS             2815..>2871
                     /locus_tag="VYR08_001938"
                     /inference="COORDINATES: protein motif:HMM:NF019384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEV3467411.1"
                     /translation="MIVGNLGAQKEKRNDTPIS"
BASE COUNT         1008 a          470 c          607 g          786 t
ORIGIN      
        1 caggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
       61 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
      121 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
      181 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
      241 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
      301 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
      361 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
      421 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattaagc aattgcttaa
      481 gctgccagct gaatgctttc atcctaaacc aaaagtaaac agtgccttaa taaaacttac
      541 ccgccatacc acagatgttc cagataaata atagaatggg caaagcataa aaacttgcat
      601 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
      661 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
      721 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
      781 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
      841 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
      901 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
      961 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     1021 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     1081 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
     1141 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     1201 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     1261 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     1321 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     1381 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     1441 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
     1501 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc
     1561 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
     1621 tggatgaatt gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt
     1681 tagacatcta atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc
     1741 ttttctaaaa gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga
     1801 ggtttgtgag tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct
     1861 taagaacgca taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag
     1921 gattaacaga gtaacctcta ttcttattca gctgcaatca aaaaaaaata attcctgcca
     1981 aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat atccggacac
     2041 ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg
     2101 gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag
     2161 ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt
     2221 ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca
     2281 gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag
     2341 caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag
     2401 ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat gaacagaact
     2461 ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg tggatcggat
     2521 taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt tggaacaaat
     2581 attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt gtaaaaattg
     2641 gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag ccctttaaag
     2701 agggctttta tatattaatc acaaatcact tatcacaaat cacttatcac aaatcacaag
     2761 tgatttgtga ttgttgatga taaaataaga ataagaagaa atagaaagaa gtgagtgatt
     2821 gtgggaaatt taggcgcaca aaaagaaaaa cgaaatgata caccaatcag t
//
LOCUS       DAVMHG010000166         2835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVMHG010000166 DAVMHG010000000
VERSION     DAVMHG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.168"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            6..248
                     /locus_tag="VYR08_001939"
     CDS             6..248
                     /locus_tag="VYR08_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467412.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYNVPILKNEYTVRLLLLFG
                     FEIEDPEVIEYLVNAWKYRELVLHSVRMYDMEREAS"
     gene            275..652
                     /locus_tag="VYR08_001940"
     CDS             275..652
                     /locus_tag="VYR08_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV3467413.1"
                     /translation="MIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREVDFVN
                     MVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLLESRAQR
                     AMRENNAGQDLADLVLEEEELPF"
     gene            808..1092
                     /gene="groES"
                     /locus_tag="VYR08_001941"
     CDS             808..1092
                     /gene="groES"
                     /locus_tag="VYR08_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV3467414.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            1108..2730
                     /gene="groL"
                     /locus_tag="VYR08_001942"
     CDS             1108..2730
                     /gene="groL"
                     /locus_tag="VYR08_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV3467415.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
BASE COUNT          883 a          525 c          673 g          754 t
ORIGIN      
        1 taaaaatgaa gattttaaat attgaactag caaatgtaga gcagacagat ttaggttttg
       61 agcattgggt agatgtgact tacaacgtcc caattttgaa aaacgaatac acggttagat
      121 tgttgctgct ctttggtttt gaaattgaag atcctgaagt gattgaatac ctggtcaacg
      181 cttggaaata tcgtgagctg gtcttacatt ctgtgaggat gtatgatatg gagagagaag
      241 caagttaaaa tactgttact aactctaact tatcttgatt ggacgtttaa cgtctacacc
      301 agaattgcac aaaaccaaca atgacaagtc agtagcgcga gcaactatcg ctgtcaaccg
      361 tcgttacaaa gaccaaaacg gtgaacgtga agttgatttt gtcaatatgg tcctatgggg
      421 cagactagca gaaactttgg caagctacgc aaccaaaggt agtctcattt ccgttgatgg
      481 agaattgcgt acccgtcgct ttgagaaaaa tggtcagatg aattatgtaa ccgaagtcct
      541 tgtgacagga ttccaactct tggaaagtcg tgcccaacgt gctatgcgtg aaaataatgc
      601 aggccaagat ttggcagatt tggtcttgga agaggaagaa ttgccatttt aagaattaaa
      661 aagtctgagt tggtctcagg ctttttatct tgagaaagtc agactttttt cttgattatt
      721 tctgactaag tgatacaata gaactatgaa ttagcactca ggtataaaga gtgctaataa
      781 tatctatctc attatggagg aaatcagatg ttgaaaccat taggggaccg tttggtctta
      841 aaagtagaag aaaaagaaca aactgttgga ggctttgttc tcgcaggttc agcccaagaa
      901 aaaaccaaaa cagcccaagt tgtggctact ggacaaggtg ttcgtacctt gaacggtgac
      961 ttggttgctc caagtgttaa aactggagac cgtgtcttag ttgaagccca cgcaggtctt
     1021 gatgtcaaag atggcgatga aaagtatatc atcgtaggcg aagctaacat cttggcaatc
     1081 attgaagaat agaaggagaa agtaagtatg tcaaaagaaa ttaaattttc atcagatgcc
     1141 cgttcagcta tggtccgtgg tgtcgatatc cttgcagata ctgttaaagt aaccttggga
     1201 ccaaaaggtc gcaatgtcgt tcttgaaaaa tcatttggct caccattgat taccaatgac
     1261 ggtgtgacta ttgccaaaga aattgaatta gaagaccatt ttgaaaatat gggtgccaaa
     1321 ttggtatcag aagtagcttc taaaaccaat gatatcgcag gtgacgggac tacgactgca
     1381 acagtcttga cccaagctat cgtccgtgaa ggaatcaaaa acgtcacagc aggtgcaaat
     1441 ccaatcggta ttcgtcgtgg gattgaaaca gcagttgccg cagcagttga agctttgaaa
     1501 aacaacgcca tccctgttgc caataaagaa gctatcgctc aagttgcagc cgtatcttct
     1561 cgttctgaaa aagttggtga gtacatctct gaagcaatgg aaaaagttgg caaagacggt
     1621 gtcatcacca tcgaagagtc acgtggtatg gaaacagagc ttgaagtcgt agaaggaatg
     1681 cagtttgacc gtggttacct ttcacagtac atggtgacag atagcgaaaa aatggtggct
     1741 gaccttgaaa atccgtacat tttgattaca gacaagaaaa tttccaatat ccaagagatc
     1801 ttgccacttt tggaaagcat tctccaaagc aatcgtccac tcttgattat tgcggatgat
     1861 gtggatggcg aggctcttcc aactcttgtt ttgaacaaga ttcgtggaac cttcaacgta
     1921 gtagcagtca aggcacctgg ttttggtgac cgtcgcaaag ccatgcttga agatatcgcc
     1981 atcttaacag gcggaacagt tatcacagaa gaccttggtc ttgagttgaa agatgcgaca
     2041 attgaagctc ttggtcaagc agcgagagtg accgtggaca aagatagcac ggttattgta
     2101 gaaggtgcag gaaatcctga agcgatttct caccgtgttg cggttatcaa gtctcaaatc
     2161 gaaactacaa cttctgaatt tgaccgtgaa aaattgcaag aacgcttggc caaattgtca
     2221 ggtggtgtag cggttattaa ggtcggagcc gcaactgaaa ctgagttgaa agaaatgaaa
     2281 ctccgcattg aagatgccct caacgctact cgtgcagctg ttgaagaagg tattgttgca
     2341 ggcggtggaa cagctcttgc caatgtgatt ccagctgttg ctaccttgga attgacagga
     2401 gatgaagcaa caggacgtaa tattgttctc cgtgctttgg aagaacccgt tcgtcaaatt
     2461 gctcacaatg caggatttga aggatctatc gttatcgatc gtttgaaaaa tgctgagctt
     2521 ggtataggat ttaacgcagc aactggcgag tgggttaaca tgattgatca aggtatcatt
     2581 gatccagtta aagtgagtcg ttcagcccta caaaatgcag catctgtagc cagcttgatt
     2641 ttgacaacag aagcagtcgt agccaataaa ccagaaccag tagccccagc tccagcaatg
     2701 gatccaagta tgatgggcgg gatgatgtaa gctttctata gaaaacaact tataaaaaac
     2761 acaaaaggag ggaatgacta acccttcttt ttataggctc tttgtcaact gtagtgggtt
     2821 gaagtcagct aagct
//
LOCUS       DAVMHG010000167         2832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVMHG010000167 DAVMHG010000000
VERSION     DAVMHG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.169"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            98..1105
                     /locus_tag="VYR08_001943"
     CDS             98..1105
                     /locus_tag="VYR08_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV3467416.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1108..1308
                     /locus_tag="VYR08_001944"
     CDS             1108..1308
                     /locus_tag="VYR08_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV3467417.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1460..1642
                     /gene="rpmF"
                     /locus_tag="VYR08_001945"
     CDS             1460..1642
                     /gene="rpmF"
                     /locus_tag="VYR08_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV3467418.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1658..1807
                     /gene="rpmG"
                     /locus_tag="VYR08_001946"
     CDS             1658..1807
                     /gene="rpmG"
                     /locus_tag="VYR08_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV3467419.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(1899..2459)
                     /locus_tag="VYR08_001947"
     CDS             complement(1899..2459)
                     /locus_tag="VYR08_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3467420.1"
                     /translation="MNISGERAVEGLRDEKERYRAILKTKESVRTIPMSPKVEQILLK
                     YIERNKFQARFNPTYHDMGYIFTRVYIKGKNQQGSPLYHTEISMFLRGGTSQSVKYNK
                     KSGKSYKDIDDLVDFGRPVHIVPHMFRHSFVSVMADKNVSLNVIREFVGHSEDSREIE
                     KIYLHVMQKGKHKIEQAMIDLSKIIS"
     gene            complement(2535..2825)
                     /locus_tag="VYR08_001948"
     CDS             complement(2535..2825)
                     /locus_tag="VYR08_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467421.1"
                     /translation="MEGMVFPNSHGVKRVQRFNPDESPCFLLDDESRELLMRKLPFDK
                     INEPTLKKFAENIIVLNRQKHRVSDKSRMVLMNEANYSYSGESFYTTIVEYY"
BASE COUNT          913 a          564 c          480 g          875 t
ORIGIN      
        1 ttgggctgaa actttttagt gattattttc aactttctat tgacttttaa aaacaaagtg
       61 atatcattta gatatcaaat aacaggaggt cattcttatg actgaaaaac taatcaattc
      121 aaaaccaaat ggtgtattcg cattgattct cattgaattg acgctcgtac ttggtgtctt
      181 tatatttata atgggtgttg gttcggaaaa catttttgga attattatcg gacctttact
      241 aatcgtaatt gcaggtctag ctcatgctgg tttaaaagtt gtcaaacctc aggaagctct
      301 ggttctgaca ctctttggta attatacagg taccatcaaa gaacctggtt tttactttgt
      361 caatcccttc agcgtagcag tcaaccctgc aaaccacact cgacttggac aaagtggtga
      421 tgttagcaca aaatctcctt ttttaggagc taaatcatca aatgacaatg atgtaaatct
      481 tgaaattggc aagaaacaca tttccctcaa agtcatgacc ttgagcaatt ctcgtcaaaa
      541 aatcaatgat tgcttaggaa accctgtaga aatcggtatc gcggtaactt ggagagttgt
      601 cgataccgct aaggcagtct tcaacgttga taactacaaa gaatatcttt cattgcaatg
      661 tgatagcgcc ctccgtaata ttgtccgcat ctatccttac gatgtgtctc ctaatgtgga
      721 tactacgggt gacgggcaag cagatgaagg tagtctccgt ggctctagcg aaattgttgc
      781 taaccgtatt cgtgaagaaa tccaaagtcg tgttgaggat gctggcttgg aaatccttga
      841 agcacgtatc acttacctag cttatgctcc agaaattgct gccgttatgc ttcaacgcca
      901 acaagcatct gccattattg atgcacggaa gatgattgta gatggtgctg taggaatggt
      961 tgaaatggca ctagaacggc tcaatgaagg ggaattggta gaacttgacg aagaacgaaa
     1021 agctgccatg gtttcaaatc tccttgtcgt tctttgtggc aatcatgatg cacaaccaat
     1081 tgtcaacaca ggaagtcttt actaaagatg gctgaagcta aaaaaaagca gatccccctt
     1141 cgactctcaa caaagttata cgctgcactc gcatcatggg cagaagacga ctttcgttca
     1201 gtcaatgggc aaatcgaata tctcctcaca gagtgtgtca agcaacggaa gaaagacgga
     1261 aaatacgtat cagaaaccat tgacgaacca tttgagattg atatttaaca tcttctcctg
     1321 tctgacaagt cggacaggag aattttttca tctttttttg tcaagtaact ttactttaca
     1381 aaaaaaatgt gttatcctag tatggttgat gaaaatcagt agattgaatc gaatataaat
     1441 acctaaagga gaaatcaaaa tggcagtacc tgcacgtcgc acttcaaaag cgaagaaaaa
     1501 caaacgtcgt acacactaca aagtaacagc tccatctgta aactttgacg aaactactgg
     1561 agattactca cgttctcacc gtgtatcact taaaggatac tacaaaggac gtaaaatcgc
     1621 taaagctgca tcagctgaat aatagaaggg agataccatg cgcgtaaata ttacacttga
     1681 acacaaagaa tctggtgaac gcttgtacct tacttctaaa aacaaacgta acactccaga
     1741 ccgccttcaa ttgaagaaat actcaccaaa acttcgcaaa cacgttgtgt ttacagaagt
     1801 taaataatac aacatacaga aagcctatga aatcaacatt tctaaggctt tttcttttaa
     1861 gtttagcaaa ataactgaat tttaactgat tataataatc aagagattat tttagacaaa
     1921 tctatcatag cttgttcaat cttatgtttt cctttttgca taacatgaag atagattttt
     1981 tctatttctc tactatcttc tgaatgtccc acaaactctc gaataacgtt taagcttaca
     2041 ttcttatcag ccatgactga aacaaaacta tgtcggaaca tatggggaac tatgtgaaca
     2101 ggcctaccaa aatcaactag gtcatcaata tccttatagg attttccact tttcttatta
     2161 tacttaacgg actgactagt tccacctctt aagaacattg aaatttctgt atgatacaat
     2221 ggactgccct gctggttctt cccttttatg tataccctag taaaaatata tcccatatca
     2281 tgataagtcg gattaaatct cgcctgaaac ttatttcttt caatatattt aagtaaaatt
     2341 tgttctactt ttggtgacat cggaatagtt ctaacgcttt ctttagtttt tagaattgct
     2401 ctatatcgtt ccttctcatc acgcaagcct tcgacagctc gttctccaga aatattcatt
     2461 aactttcatt tttatatttt tcctttcaat tttcaaaaac aaagggaagg attaacctaa
     2521 cctcccctac caagttaata atattcgaca atagtagtat agaatgattc accactatag
     2581 ctgtagttag cctcattcat caataccatt ctagatttat cagatacacg atgcttttga
     2641 cgatttaata caataatatt ctcagcaaat tttttcaaag ttggttcatt aattttatca
     2701 aatggtaatt ttctcataag taactcccta gattcatcgt ctaaaagaaa gcatggtgac
     2761 tcgtctggat taaacctctg tacacgtttc accccatgag aattaggaaa gaccattccc
     2821 tcaatatcaa cc
//
LOCUS       DAVMHG010000168         2807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVMHG010000168 DAVMHG010000000
VERSION     DAVMHG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.170"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..383)
                     /locus_tag="VYR08_001949"
     CDS             complement(<1..383)
                     /locus_tag="VYR08_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467422.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCIGDFPHDFF
                     DTYLDHVAKYAYEQKVNNIKEYYPL"
     gene            complement(441..1154)
                     /locus_tag="VYR08_001950"
     CDS             complement(441..1154)
                     /locus_tag="VYR08_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV3467423.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLTQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1613..1993)
                     /locus_tag="VYR08_001951"
     CDS             complement(1613..1993)
                     /locus_tag="VYR08_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV3467424.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2415..>2807)
                     /locus_tag="VYR08_001952"
     CDS             complement(2415..>2807)
                     /locus_tag="VYR08_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV3467425.1"
                     /translation="DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLK
                     NNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          853 a          544 c          447 g          963 t
ORIGIN      
        1 tttaaaggat aatactcttt aatattatta actttttgtt cgtaggcata ttttgctaca
       61 tgatctaggt atgtatcaaa aaagtcatga ggaaaatctc ctatacagcc attacgattc
      121 atattgaggc tatattttcg attagtaaga taattcggaa atatatttcc tattactcta
      181 agattatcta cttgtgaatc ccttctccca atataatccc cttctttgac taatgatgga
      241 gatttccaag taaaaattaa atcgccagag aaatttttct tatcaaaaaa cttaaaaaga
      301 gattgataaa tactatttgc taattgacaa gtgtcagatg taaattcata attaacagct
      361 tcattaaaat caacttcaga catttcaatg tactcttcaa tagtaatatt agcttttttg
      421 tagtcattag ctcgtctcct ttaaaatgtg atcttttcaa gaatattttt atctaaacaa
      481 tccagcaagt cttggtaaga atagacttcg taagtcggct gggcttgtgt gtgattttcg
      541 aggtgatgag gattatacca gatagtgtca atccccgcat tattgccacc ttgaatgtcg
      601 gcggttagag aatctccaat catcagcgtc ttttctttac taaatccagc aatttgctgg
      661 ccaatctttt cataaaaaag agcatccggc ttttgagttt gcaactgttc tgagataaag
      721 acttgattga aataaggtgc tagaccagat tgagtcaaac gtcctgtctg aatggcagta
      781 atgccatttg tcgcagcata caagttataa tcacgctcaa tgaggctgtc caagagatca
      841 tgagcgcccg atagtgtttg tccctgctgg gcgaggtaaa attggtaacg ctgggcaaga
      901 aaactaccgt ctttttcctg tccaaaatga gcaaataaac gagaaaatcg cgtgttaacc
      961 agctcttgtt tactgatttt cttcagctcc aagtctttcc agagagcctt gttcatagga
     1021 acgtaataat ctttataagc ctgaatatcc gcaactcctt cttcttttag aagttgggtc
     1081 aaagccacat cctcagcagc atcaaaatca agaagagtgt ggtcgaggtc gaagagtaga
     1141 aatttgtaga acaatttgag gttttccttt ctgaaaattc attaagaaca ttatatcata
     1201 aagcacctca tacaattaac taatttaatc acttaaaaaa aattcgaaca ctttctatac
     1261 aactgacagc tcaaatcttt cagaatagaa caatactaac tatcgagcac cccgtcttca
     1321 taaatatatg tgtaattcta gacctagaat tcctataaac taaatgcttt catactcttc
     1381 caagtaattg attgtcttaa attttaattt ttgaaggatt ctaaagctag aatagtccca
     1441 tcacaatcag ttttgattga ttcacaattt agaaacacta tagtttcact cctgttaaaa
     1501 taaaaaggaa ctgcataaag caatcccttt ctgattttga aatcatttac ttaacatttt
     1561 atagttgaga taatcaatag cttacctata aaaagagtta tagtaaaatt ccttatttat
     1621 tgatttcaag ctccgctaac tgtgtttgaa taactgacag ttctgcacca gcctgaagaa
     1681 gagcagctgc attataggca ccttctacaa ttggaaccct gttgatgatg atacttttat
     1741 cactgaaatc agtcaccatt tttaagttca ttttagcaga acctaggtca aaaaaggcaa
     1801 gtaaagtatc tgctggattt tcggaaacaa ccctatctac ttgatcaaaa ctcgttccaa
     1861 ttcctccgcc ctcggttcct cctacataag taatcggaac atctttagct actttactaa
     1921 tcagttcaac aacaccttct gcaatgtgtt tggaatgtga aacgataaca agaccaatac
     1981 caatactttc catcaaacca ccccagtttc taaaatagca gtaaagagta atcctgatga
     2041 gaatgatcca ggatcaatat gtccaagaaa ccacatgctc ctaagacaag agctaacaga
     2101 ctggccatca ataatagtat tgttcttttt ttcatcatta ctccttaact agtgtttaac
     2161 tgatcaatta gccagtaaat agtttatctt tatttacact atctgttaag atatagtaaa
     2221 atgaaataag aacaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtata
     2281 ctattctagt ttcaatctac tatattttgc acattttcat gaaaaaaatg agaactagaa
     2341 ctcacattct gctctcattt ttcgttttcc cgttctccta tcctgttttt aggagttaga
     2401 aaatgctgct acctttactt actctccttt aataaagcca atagtttttc agcttctgcc
     2461 ataatagtat tgttgtcctg ggtgccaaat agtaaattat tttttaatcc tgtgagagtc
     2521 tctttggcat tggacttgat aattggatcc tggatttttc caagtaaatc ttcagcctct
     2581 ctcagttttt cttcaacctt ttcagtctcg acctgaggtt cttctgattc ctctggtgat
     2641 tcttcttctg gttcctctgt tggttttgga gactctggtt tctcgctttg cggtttctct
     2701 tctcgagggg tttcttcctc aggtttttct gtctgaggtt tctcctcgct tggtttttcc
     2761 gtttgattgg tatcagcttg accatttttg tttctttgaa catggtc
//
LOCUS       DAVMHG010000169         2749 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVMHG010000169 DAVMHG010000000
VERSION     DAVMHG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.171"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(242..1252)
                     /gene="galE"
                     /locus_tag="VYR08_001953"
     CDS             complement(242..1252)
                     /gene="galE"
                     /locus_tag="VYR08_001953"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV3467426.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIEAAIHFAGYKAVGESVQKPVMYYENNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGVHNHSPLIETMPTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPFIAQVAVGKRPELSVFG
                     DDYDTVDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKVPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            complement(1265..2740)
                     /gene="galT"
                     /locus_tag="VYR08_001954"
     CDS             complement(1265..2740)
                     /gene="galT"
                     /locus_tag="VYR08_001954"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV3467427.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
BASE COUNT          818 a          572 c          379 g          980 t
ORIGIN      
        1 ctggaaaatg attaaattct ccattttctt tgaacttaat ttttgttagg tttttcatat
       61 aaagaccctc ttttttatat ttgacatcgg tgtcattatg tttacattat acaatgaaac
      121 cgttcacttt gtcaagcgct ttcaaacatt tttttaaaaa aatttatctt tcattaaata
      181 tgcatttcat taaaaggtta agtaattttt ccaaaataca gcactcatat agacagtatc
      241 atcaattata gccattggga ttttttgatt gccaattcca tgtatctcta cacatgtctt
      301 caatcgattt tgttgtcctc caatttaatt ccttatatgc tttgtctgca tttgcataac
      361 aagtcgcaac gtctcctgat cgtcttggta caattttata cggaattgga accttattaa
      421 cactttcaaa tgtatttaca agttgtaata cacttgtgcc ttctcctgaa ccaaggttat
      481 aaatataaac atctgttttt tcagatactt tttctaaagc ttttatatgc cctatcgcta
      541 aatccactac atggatataa tcgcgaacac cagtaccatc taccgtatca taatcatctc
      601 caaaaacact tagctctggt cgcttaccta ccgctacttg tgcaataaaa ggcattaagt
      661 tgttaggaat gcctgatgga tcttctccaa tcaaccccga ctcatgagca ccaatcggat
      721 tgaaataacg aagcaacgca atactccact ctgaatctgc tacatgaaca tcttttaaaa
      781 tttgttcaag catcactttt gtgtacccat acggattcgt tgcacttgtc ggcatcgtct
      841 caataagggg agaatgattg tgaacgccgt atacagtcgc actcgatgaa aatacaatct
      901 ttttaacatt aaactctgac atcacttcaa caagagctaa tgtgctcata atattatttt
      961 cgtaatacat tacaggcttt tgcacggatt cgccgacagc tttgtatcct gcaaaatgaa
     1021 ttgcagcctc aatagattct tgttcaaaaa cttttctcaa tgctggttta tcacaaacat
     1081 ctaattcgta aaacacggga cgtattcctg taattgcttc aatacggtct agcaccaaga
     1141 tgctagagtt cgaaaggttg tcgacaatga tgacttcctt tcctaaattt agtaattcta
     1201 ctacggtatg gctaccaata taaccagctc cgcctgttac caatattgcc atttttgttt
     1261 cctcctaatt aattccaacc gacttaacaa atctcataaa cgcttcatgc ccagacggtg
     1321 tattcttata aactcctgca tcttccagaa ctctcgcaaa cacttgtcct gcttcgtgtt
     1381 gaactacgct attaacctct tctttattaa tgcgaggata tttttctttc aattggtcgg
     1441 cccattctaa atgataatcc gcaattgcat tatcctctcc taaaagatat tttccaactt
     1501 cttctaactc tggtttcaaa cgaggtggta atatcgcaag tcccatcact tcgattaacc
     1561 cgatattttc ctttttaata tgttgtacat cttgatgagg atggaaaaca ccatctgggt
     1621 attgttcagt agtatgatta tctcttagaa caatatctaa ttcgtatctc ccgtccactt
     1681 tacgagcaat aggagtcacc gtatggtgtg ggacatcttc agtcatagca atgatgtcta
     1741 cttctaaatc tgaatattct ctccacttat ttagaatttt agtagctaaa tctaacaagc
     1801 gatttttatt ttcactttgt aacctaatta ctgacattgg ccattttaca ataccagcat
     1861 taacatcctc aaagtcttta aaacaaaatt cactctcaaa ttttgctttt tccattggga
     1921 aaatatgttt ccctccctgg tagtggttat gactaagaat ggagcctcct gagataggaa
     1981 gatcagaatt tgaaccagca aaatatcctg gcaaaatatc aacaatctcc aataattgtt
     2041 caaatgtttt agaggtaata gccattggta catgttgact attcaaaaat atcgcatgct
     2101 cattaaagta tgagtaggga gaatactgga atccccatac ttcgtcacca agtttcaacc
     2161 gaataattct atgattcgaa cgtgctggat aatttattcg cccctgatat ccttcatttt
     2221 ccatacatag taaacatttg ggataattag ttgcttttac taatttttca gcagcaattg
     2281 tttttggatc tttttcgggt tttgacaaat ttatcgtaat ctctagctct ccgtatttag
     2341 ttgatgctcg aaactcaata ttcttagcaa tagcagaagt tttaatataa tcactatctt
     2401 tacttaactt ataaaactct tcaactgctt cttgaggtga tatatcatat gaactccaaa
     2461 aaatatcatt taatcgacta ggtaaaggaa ctatgaaatt cattaactct gctcctaaac
     2521 attccttttc ctcgattaaa tctttaattt taccgttttt taaggcgatt tccactaagt
     2581 aatcttttat ttgtttcagg tcattttcat cggaaatgcg atcaattccc tcctcaccta
     2641 ttaacgctag tactctattt ttcacatata ttttgtcaat ttcattatac attccgtatt
     2701 caattactct atcaacaaaa ttatcaataa ttgttttcat atatttttc
//
LOCUS       DAVMHG010000170         2743 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVMHG010000170 DAVMHG010000000
VERSION     DAVMHG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.172"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..853
                     /locus_tag="VYR08_001955"
     CDS             26..853
                     /locus_tag="VYR08_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3467428.1"
                     /translation="MPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLS
                     VVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMAL
                     SSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNAL
                     AMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDI
                     RTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
     gene            853..1860
                     /locus_tag="VYR08_001956"
     CDS             853..1860
                     /locus_tag="VYR08_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3467429.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            1860..2654
                     /locus_tag="VYR08_001957"
     CDS             1860..2654
                     /locus_tag="VYR08_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467430.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          915 a          422 c          475 g          931 t
ORIGIN      
        1 tattaaatca attatatata aaagaatacc tagaactatt tttgcaattt tagcaggttc
       61 tagtcttgcc ataagcggtg tattgatgca atcagttact agaaacccaa tagctgatcc
      121 aggtatactc ggtataaaca caggagcaag tcttagtgta gtaattggtc tttctttttt
      181 aggaatttca tcaagcataa gccatataag ttttgcaatc attggtggct tagtaagtgc
      241 aatttttgta tacgcgattg ctgtaagcgg aaaagcaggc cttaccccta taaaacttgc
      301 cttatcagga acttgtgtta gtatggcttt aagcagtttt gtaagttttt taattttacc
      361 gaataataac gtcttagaca aatttagatt ttggcaaata ggtagccttg gagcagctac
      421 attatcttct atatctacac tactaccttt tataatttta ggtcacttga tagctatatt
      481 tatttcatca gatttaaacg ctttagctat gggtgatgaa atggctgttg gtcttggagt
      541 taatgttaat aggataagat cacttgcaat aattgcaagt gtgcttttat gttcaagtat
      601 tactgcaatt ggtggaccta ttggcttcgt aggtcttata gttcctcact tttgtggctt
      661 atttataagc aaagatatac gcacaatgac catttcttca tcttttatag gtgcagagct
      721 cttgcttata tgtgatataa tcggccgtat gttaggtaaa ccaggtgaaa ttgaagtagg
      781 gataattact gcaataatcg ggggtccagt acttatttat gtaactatga aaaatagagg
      841 ggttaataac taatgcaaaa tttaattata ggtattcaaa aaagaaaaaa tagaataaca
      901 ctattttcct cactattttt attaataata atcagtctat catttttcat tttacttatc
      961 ggagatgaaa gttattcttt ttcaactttg attaaagtct taaatagtga aactgttcct
     1021 ggagctagtt tttcgattat ggaaattaga ttaccaaaat tattagcagg aattatagct
     1081 ggctggtctt ttggattggc aggatttatc tttcaaacta tgttaagaaa tcctcttgca
     1141 agtcctgata taatcggtgt cacaagttct tcatctattg cagcggtctt ttgcatattg
     1201 gtattaaaaa caaatagttt aactactgga attatttcaa taacttgtgg actaacatca
     1261 tctttaatat tatttttact agctaaaaaa gatggttttt cagcagcaag actgataata
     1321 ttaggtattg gttttcaagc tgtcacaaga gcaggcacct catttttatt gttgaaagta
     1381 gcaagatatg aattacaaga agttatgaga tggctcagtg gctctttatc ttttacaaag
     1441 ttagatgaca tacctcttgt tctaatagta agtattattg ctactatatt agttttattt
     1501 tttaataaaa gactagaaat tattgaactt ggtgaagaaa tagcaatcgg acttggagca
     1561 aatcccgagc tttcaaggct tgttttaatt ttttgcgctg tatctttaac tgctttttct
     1621 acttcaatta caggaccaat agcttgtata tcttttttag ctggtcccat agccttaaat
     1681 attggcaaga aaagaagccc aatattagct ggattggttg gaattttact agttttgtta
     1741 tcagacatat tctctcaaaa tattttacca gctagatatc cagtaggtgt tgtaactggc
     1801 ttgttaggtt caccatactt aatatactta ctaataaaaa tgaacaggag gaatatataa
     1861 tgaaaggtct gtggtcaaat aatcttacct gcggttatga tgaaaaaata atcttggaaa
     1921 atataaatat aaaaatacct gaagaaaaaa tatcagttat tattgggtca aatggttgtg
     1981 ggaaatcaac actcattaaa accttgtctc gacttataaa gccattagag ggagaagtat
     2041 tgcttgataa taaatcaatt aattcttata aagaaaaaga tttagcaaaa cacatagcta
     2101 tattacctca atctccaata atccctgaat caataacagt agctgatctt gtaagccgtg
     2161 gtcgtttccc ctacagaaag ccttttaaga gtcttggaaa agatgacctt gaaataataa
     2221 acagatcaat ggttaaggcc aatgttgaag atctagcaaa taacctagtt gaagaacttt
     2281 ctgggggtca aaggcaaaga gtatggatag ctctagccct agcccaagat acaagtatcc
     2341 tacttttaga tgagccaact acttacttgg atatctcata tcaaatagaa ctattagacc
     2401 tcttgactga tctaaaccaa aaatataaga caaccatttg catgattttg cacgatataa
     2461 atctaacagc aagatacgct gattacctat ttgcaattaa agaaggtaaa cttgttgcag
     2521 agggaaagcc tgaagatata ctaaatgata aactagttaa agatatcttt aatcttgaag
     2581 caaaaattat acgtgaccct atttccaatt cgcctctaat gattcctatt ggcaagcacc
     2641 atgttagctc ttaataattg aatatcggaa ttcatttaaa agtcgcaaat tttgaactga
     2701 ccctcaaaag ttaggccaaa aaactaacta aaggaggtca gtt
//
LOCUS       DAVMHG010000171         2667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVMHG010000171 DAVMHG010000000
VERSION     DAVMHG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.173"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..582
                     /locus_tag="VYR08_001958"
     CDS             <1..582
                     /locus_tag="VYR08_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467431.1"
                     /translation="NKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKNVTK
                     NGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGM
                     VILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFA
                     DFVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            642..878
                     /locus_tag="VYR08_001959"
     CDS             642..878
                     /locus_tag="VYR08_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV3467432.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            875..1288
                     /locus_tag="VYR08_001960"
     CDS             875..1288
                     /locus_tag="VYR08_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV3467433.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1406..2371
                     /locus_tag="VYR08_001961"
     CDS             1406..2371
                     /locus_tag="VYR08_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3467434.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            2405..>2667
                     /locus_tag="VYR08_001962"
     CDS             2405..>2667
                     /locus_tag="VYR08_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467435.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWRGRSAVLNQHIFKV"
BASE COUNT          969 a          396 c          516 g          786 t
ORIGIN      
        1 aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga taggggcaaa
       61 aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt aaatacaaag
      121 aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa aacaaaggat
      181 aaattacttc gaaaaggcaa acttgagcgt tatgatatag tcttgacaac aagaggtact
      241 gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg tataaattca
      301 ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat tatccatgtt
      361 ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca gttaccaatt
      421 acaaaattaa aaaaaatact tctccccctt cccccactag ccctccaaaa tgagttcgca
      481 gactttgtag ccctggtcga caaatcacaa ttggcaatcc aaaaatctct ggaagaactt
      541 gaaactttga agaaatctct gatgcaggag tattttggct gatattctgc cattgtaatt
      601 acggtaatga tttgttataa tacttcaaag gaggaaatca gatggtagta aaaacaagaa
      661 aacaaggaaa ttcaatcacc attacgattc caagtgaatt taatattcca agtggtgtta
      721 aatacgaagc gaaattgtta ccaagtggtg agattatctt tactcctgaa gaattggggc
      781 agcaggtttc ttatgtatct gatgatgcct ttgacttaaa tttagataaa atatttgacg
      841 aatacgacga tgttttcaaa gctttggtgg aaaaatgaca atctatctga cagaaaagca
      901 aattgaaaaa ataaatgctt tagcaattca acggtattct ccaaatgaga aaattcaaac
      961 agttagttct tctgccttaa atatgattgt gaacttacca gaacaatttg tctttgggaa
     1021 gtctctttat ccaacaattt ttgataaagc aacgatacta tttgtccaat tgataaagaa
     1081 gcatgttttt gctaatgcta ataaaagaac tgctttcttc gttttggtca aatttttaca
     1141 attaaacggc tatcgttttt ctgtaacggt agaagaagca gtaaaaatgt gtgtaaccat
     1201 cgcagtagaa gctttaactg atgaaaaaat gacaagctac tccaaatggg tttctgaaca
     1261 ttctgttaga gaaaaggtca aaaagtaacc tagtatgctg gatttgaatg agcacaagaa
     1321 aataaatgaa cagacaatat tagaattctg taatgcagaa actgatattg tctcttttta
     1381 ttgatgaata agaaagtgag aaattatgga atcaaaagtt acaattatca tgcaagaaat
     1441 gttacctctt ttaaataatg aacaattact agcgttgaga gagagtttag aacatcatct
     1501 agtagacgga aaaaagcagc agaagtattc gaataataac ctgttgcaac tatttattac
     1561 cgccaagcag gtagagggct gtagctcaaa aacaattcgt tattaccaga ggacgattga
     1621 aaacttgttt aatgctatta aagagtctgt gacacaactc acaacagatg atttaaggag
     1681 ttatttagca aattaccagt ctgaaaagga ttgtagtaag gcaaatttag acaatattag
     1741 gcgtatattg tcttcttttt ttgcttggct tgagcaagag gaatatatca ttaaaaatcc
     1801 cattcgacgg ataaagaaaa ttaagactga gcaaaatgtg aaggaaactt atactgatga
     1861 acatttggaa attatgcgtg ataactgtga aaatttgaga gatttggcaa taatagacct
     1921 actagcatcg acaggtatgc gtgtagggga gcttgtacag ttgaatcgtt cagatattga
     1981 ttttgaaaac agagagtgtg ttgtctttgg taaaggaaag aaggagagac cagtatattt
     2041 tgatgctcgg acgaaaattc atttaagaaa ttatcttaac gacagaaaag atagtcaccc
     2101 tgctcttttt gtaacgctag ttggaaaagc ccagagactt ggaattgctg gtgtagagat
     2161 tcgcttaaga aagttaggag acaaactcgg catacaaaag gttcacccac ataagttcag
     2221 aagaacttta gcgactaagg caattgataa aggtatgcct atcgaacaag tccaaaaact
     2281 gctaggtcat agcaagattg acacaaccct ggcctatgcc atggtcaatc aaaataatgt
     2341 caagcattca caccaaaaat tcatctctta aaagcaaatc ccgatattcc gaggaggtgg
     2401 ttgcatgaag aaggtgaaat taggtgaagt ggctactttt atcaatggct atgcttttaa
     2461 acctcaagat tggtcatctg aagggaaaga gattatcaga atacagaatc tgactaaaac
     2521 ttcaacagag ataaattatt attcgggtac gattgataaa aaatacattg ttgaagctgg
     2581 tgatattctt atttcgtggt cgggtacatt aggggttttt cagtggcgtg gaagatccgc
     2641 agtgttaaat cagcacattt ttaaagt
//
LOCUS       DAVMHG010000172         2660 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVMHG010000172 DAVMHG010000000
VERSION     DAVMHG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.174"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            68..391
                     /gene="tnpB"
                     /locus_tag="VYR08_001963"
     CDS             68..391
                     /gene="tnpB"
                     /locus_tag="VYR08_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV3467436.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(546..854)
                     /locus_tag="VYR08_001964"
                     /pseudo
     CDS             complement(546..854)
                     /locus_tag="VYR08_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            845..1285
                     /locus_tag="VYR08_001965"
     CDS             845..1285
                     /locus_tag="VYR08_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467437.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1380..1809
                     /locus_tag="VYR08_001966"
                     /pseudo
     CDS             1380..1809
                     /locus_tag="VYR08_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1880..2038
                     /locus_tag="VYR08_001967"
     CDS             1880..2038
                     /locus_tag="VYR08_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV3467438.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            2131..2660
                     /locus_tag="VYR08_001968"
                     /pseudo
     CDS             2131..2660
                     /locus_tag="VYR08_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          810 a          497 c          555 g          798 t
ORIGIN      
        1 gaagtaagtt tttttcaatc tctcaatctc gaaatggtag aacagctttt ggataaggtg
       61 ttgctctatg acaattcatc tatctagcct aggggaggtc tatctcgtgt gtgggaaaac
      121 tgatatgaga caaggaatcg attcactggc ttatctggtt aaaacccact ttgaattgga
      181 tcctttctcc ggtcaagtct ttctcttttg tggtggacgt aaagaccgct ttaaagtcct
      241 ttactgggat ggtcaaggat tttggctact atataaacgc tttgagaacg gcagattgat
      301 ttggctaagt acagaaaagg atgtcaaagc tctcacacca gaacaagtag actggcttat
      361 gaagggcttt tctatcactc caaaaatata gtagattgaa actagaatag tacacctctg
      421 cttctaaaac attgttagaa atcgatttta ctgtcctgat cgatttgtcc tgttcttatt
      481 tcattttact ataaatccat cagaaagtcg tgatttctat tgaaatgagg actttctttt
      541 tatactcatc tgctttcaaa aagcattcta gtccatctcc gattaacgat ggactttatc
      601 acctccttct ccagtccttg tataacatct tggagttgat tcatgacatc ttccaaagtt
      661 taaaaggctt tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggg
      721 ttcatctctg gtgtgtatgg aggaataaat gcaaagccaa tattagtcgg aatctttaag
      781 gtacttgatt tatgccatat agcattgtcc ataacgagta aaagataatc atctggataa
      841 gcttgtgaaa tctcctattc ctaaagcccc tttagcgcat aactttggct cagcttctat
      901 tatcgctcac accatccatc agaagtttaa tctgaaggta cccaattatc gccaagaaga
      961 agattgggct aggatgggtt taccaatcac acgtaaggaa atctctaatt ggcatatcaa
     1021 ggcgagtcaa tactatttgg agccccttta taacctcttg cgagagagac tattgactca
     1081 gcccttactt catgcggatg aaacttctta tagggtgcta gagagtgata gtcagctgac
     1141 ttactattgg acttttttgt caggtaaagc agagaaacaa gggattacgc tttaccacca
     1201 tgatcagtgt cgaagtggtt cagtagtaca agaattccta ggagattatt ctggctatgt
     1261 gcattgtgat attttgcggc agtaacttag gactttagtc ctctagttct gcctatgcga
     1321 tagcagtcca aggtttagga gcaaggcgac gctaagcttg gtaaacttcg aaccgctcgt
     1381 ctgcttatcg tcaactggaa gaagctgaac ttgttggatg ttgggcgcat gtgagaagga
     1441 agttttttga agcgcccccc caagcaagcg gataaatcat ccttaggagc taaaggttta
     1501 gcttattgtg atcagttatt ttccttggaa agagactggg aggctttgcc agctgatgaa
     1561 cgactacaga aacgtcaaga acatctccag cccttaatgg aagacttctt tgcttagtgc
     1621 cggcgtcagt cagttttagc aggttcaaaa ctaggaaggg caattgaata cagcctcaag
     1681 tatgaagaaa cctttaagac cattttgaaa gacggacatc tggtcctttc caataatcta
     1741 gctgaacgcg ccattaaatc attggttatg ggacggagta aaagagtcca gtggactctt
     1801 ttagcctaag ctcagtttaa aaaagcgagg gtggttattt tctcaaagtt ttgaaggagc
     1861 taaagcaaga gctattatta tgagtttgtt ggaaacagct aaacgtcatc aattaaatag
     1921 cgagaaatat ctatcctatc ttctagaatg tcttccaaac gaggaaactc tcgtaaacaa
     1981 agaggtttta gaggcttatt taccatggac taaagttgta caagaaaagt gcaaataaga
     2041 aatctccaga ttaggaacta tccgtgagtt ctccagtctg gagatttttc aatagacttc
     2101 ctgcgaaaca aaacatggta taatagttct atgaatgatg aagcaagtaa acaactaacc
     2161 gatgcacgat ttaagcgtct tgttggtgtt caacgcacga cttttgaaga gatgttagct
     2221 gtattaaaaa cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagt
     2281 ctagaagacc ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaacaa
     2341 attgcggctg tttttggtat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa
     2401 gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc tgaggacacg
     2461 gtaatgattg atgcgacgga agtaaaaatc aatcgcccta aaaaaagaat tagcgaatta
     2521 ttctggtaaa aagaaatttc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
     2581 aattgtttct ttggatatca ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag
     2641 tcgcagaaat atcagacaag
//
LOCUS       DAVMHG010000173         2639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVMHG010000173 DAVMHG010000000
VERSION     DAVMHG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.175"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            14..805
                     /locus_tag="VYR08_001969"
     CDS             14..805
                     /locus_tag="VYR08_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEV3467439.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFITNNFMVNDSSSNTPQIQTIY
                     DELEPPRQGKVLNYAKRQLKEQKNEEETKINEVSEAIRLYSYDYYDHPASAGTGQYLN
                     DVRVERIELPVDVDADFVIPIKGDSMEPDYHDGDLVFIQTSVDLNDGVIGVFNYNGDA
                     YIKQLVIDEDQAYLHSLNPEYKDMPITPDTDFRIIGEVVDLYREK"
     gene            807..1007
                     /locus_tag="VYR08_001970"
     CDS             807..1007
                     /locus_tag="VYR08_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467440.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            1175..2602
                     /locus_tag="VYR08_001971"
     CDS             1175..2602
                     /locus_tag="VYR08_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV3467441.1"
                     /translation="MKTTNKVAIYVRVSTTSQVEEGYSIDEQKDKLEAYCKIKDWKIY
                     DVYVDGGFSGANTQRPELERLISDVKRKKVDIVLVYKLDRLSRSQKDTLFLIEDVFAK
                     NDVAFISLQENFDTSTPFGKASIGMLSVFAQLEREQIKERMMLGKEGRAKNGKSMSWT
                     TIPFGYDYSKETGVLSVNPTQALIVNRIFTEYLNGKPVVKIIRDLNAEGHVGRKRPWG
                     ETITKYLLKNETYLGKVKYKDKVYEGQHEPIITQELFDLVQLEVERRQISAYEKYNNP
                     RPFRAKYMLSGLMKCGYCGASLGLRYTRKDKNGISHHKYQCRNRHSKDLEKRCESGWY
                     SKEELERVVIKELERIKFDPKYKNETLAKKEETIKVEEIKKQLERINNQVSKLTELYL
                     DEIITRKELDEKNDKIKTERQFLEEQLENQKSNVLSIRKRKLTRLLKDFDVEKLSYED
                     ASKIVKNIIKEIIVTKDGMSITLDF"
BASE COUNT          961 a          477 c          563 g          638 t
ORIGIN      
        1 aggaggtaaa aatatggcaa gaggacgagg gaaattaact cctcaagata aagaggatat
       61 gaaagtcttt tctgcaaatc ttaactcaat tttatctgat agaaattgta aacaagctga
      121 gctgtctcga gcaacaggaa taccgcctag tacattgaca gggtatgtaa aaggaacttc
      181 tttgccaatc cctggtaatg ttcaaaaaat tgcagatttt tttggagttc ctaaatctgt
      241 attagatcct agatttataa ctaataattt catggtcaat gactcttctt ctaatactcc
      301 ccaaattcaa accatctacg acgaactaga accacctaga cagggcaaag tcctgaatta
      361 tgcaaagagg caactgaaag agcaaaaaaa cgaagaagaa acgaagataa acgaagtatc
      421 agaagctatt cggctctaca gttacgacta ctacgaccac ccagcttccg caggtacagg
      481 ccagtatttg aatgatgtac gagtggaacg gattgagtta ccagtagatg ttgatgctga
      541 ctttgtcatc cccatcaaag gtgactccat ggagcctgac taccacgatg gtgacctggt
      601 attcattcag acaagtgtag atttgaatga cggagttatc ggagtattca actacaacgg
      661 cgatgcttat atcaagcagc ttgttattga tgaagaccag gcttacctac atagcctaaa
      721 ccctgaatat aaagatatgc caattacacc agacaccgac ttccgaatta tcggcgaagt
      781 cgtggatttg tatagggaga aataacatga gtaacgaaag tagaccgatg gaagtgatta
      841 aacacaacct agattgcaaa tgccacagac gaagagagtg gattagagta aatgataaat
      901 ggcatgctat cgagttttcg gtagacgatc caaacgaacc tcctatgaca gaggaagaga
      961 aagccaacgt ggccttaatt cttcaacaac acttatcgaa agaataaaac caactgtttc
     1021 caaaatggaa ataattgcaa acaaaaaaag cctcacgctc tcaaagtttg gcgactccga
     1081 gcgtgaggct agtggcaaga aaaacttttc aaaagatatt accttttgag acgttttctt
     1141 gtacctattt tatcaaaaaa ggggtacaaa ttcaatgaaa acaacgaata aagtcgcaat
     1201 ctacgtcagg gtgtccacta cttcgcaggt ggaagagggt tactctatcg atgagcagaa
     1261 agacaagctt gaagcctact gtaaaatcaa agactggaaa atctacgatg tctacgttga
     1321 tggtggtttc tcaggtgcta acactcaaag acctgaattg gagcgtttaa tctcagacgt
     1381 gaagcgcaag aaagtcgata tagtgctagt ctacaagcta gaccgcctca gtcgtagcca
     1441 gaaagatact ctgtttctga ttgaagatgt gtttgctaaa aacgatgtgg catttatcag
     1501 cttgcaagaa aactttgaca cttctacgcc tttcggcaag gcttcaatcg gtatgctatc
     1561 agtattcgct cagctggagc gtgagcagat taaagagcgt atgatgttag gaaaagaagg
     1621 acgggcaaag aatggaaaat ccatgtcatg gacgacaata ccattcggct atgattactc
     1681 aaaagaaact ggcgtcttat cagtgaatcc gacccaagcg ctcattgtca accgtatttt
     1741 cacggaatac ttgaacggta agccagtagt caagatcatc agagacttaa atgctgaagg
     1801 tcacgttgga agaaaaagac cttggggcga aacaatcacg aaatacttgc tgaaaaatga
     1861 aacctatttg ggaaaagtaa agtacaaaga taaggtatac gaggggcagc atgaaccaat
     1921 tatcactcaa gaattgtttg acttagtcca gcttgaagtc gaaagaaggc agatttcagc
     1981 ctatgaaaaa tataacaatc caaggccgtt cagggcaaag tatatgcttt ctggattaat
     2041 gaaatgcgga tattgtggtg cttcacttgg tctgagatat acaagaaaag acaagaatgg
     2101 aatctctcat cataaatacc aatgcaggaa ccgacacagc aaagatttag aaaaaagatg
     2161 cgaatcaggg tggtactcta aagaggaact tgaaagggtt gtaatcaagg aacttgaaag
     2221 aattaaattt gatccaaaat ataaaaatga gacactcgct aagaaagaag agactatcaa
     2281 ggtcgaggag ataaagaaac aactcgaacg gataaataat caagtctcaa aattaacaga
     2341 attgtacttg gacgaaatca tcactcggaa agagttggac gagaaaaatg ataaaatcaa
     2401 aacagaaagg caattcctgg aagaacagct cgaaaatcaa aaatcgaatg ttctgagcat
     2461 ccgaaagaga aagctaacac ggctactaaa ggacttcgat gttgaaaaat taagctacga
     2521 agatgcttca aaaattgtca aaaatatcat aaaagaaatt attgttacaa aagacggcat
     2581 gtcgataacg ctagattttt aaagggtcta gcgtatttct gtattttagt caaagtaat
//
LOCUS       DAVMHG010000174         2634 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVMHG010000174 DAVMHG010000000
VERSION     DAVMHG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.176"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            984..1385
                     /locus_tag="VYR08_001972"
     CDS             984..1385
                     /locus_tag="VYR08_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase"
                     /protein_id="HEV3467442.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPILKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYE"
     gene            1389..1700
                     /locus_tag="VYR08_001973"
     CDS             1389..1700
                     /locus_tag="VYR08_001973"
                     /inference="COORDINATES: protein motif:HMM:NF012307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467443.1"
                     /translation="MDIDLEKFFDTVPQDRLMSLVHNIIEDGDTESLIRKYLHSGVII
                     NGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLRFVRYADDCVIRSEARQPLS
                     V"
     gene            1751..2260
                     /locus_tag="VYR08_001974"
     CDS             1751..2260
                     /locus_tag="VYR08_001974"
                     /inference="COORDINATES: protein motif:HMM:NF019984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467444.1"
                     /translation="MTKTKITRPRELKYLGFGFWKSSDGWKSRPHQDSVRRFKFKLKK
                     LTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMKSIVASIDERLRTRLRVIIWKQWKK
                     KSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQKSVLKRAISKPVLEKRGLVSCLDYY
                     LERHALKVS"
BASE COUNT          859 a          449 c          582 g          744 t
ORIGIN      
        1 tttttatgtt tgatcgttat cgaaactaca ggcttgttgt tgttgaaaag aggtctcgaa
       61 atgggttatt tagacacaga agctattatc ctcgcagttt tttcatttgc tttttacaac
      121 ctatgttcat tcgcttgggt ctgctctaca ataaaaaaca ataaaaaata aatagacgta
      181 ttttcaaaaa aaaaaatgca tatttatatt agcaaaatga cgatttaaat cgtcgttttt
      241 ttgtagtacg acgggcatgt cgtatatctg aggtgtaagt cctcggtggg cacctgctac
      301 cggtgaaccc aatagcgatt cccaagcctg actatcgtga ggtagcggat taaaacggtc
      361 tggggataga ccgttttaag tctgacgcag gaaataagaa tcgtcagagg aagggatagc
      421 gaaatcgtgg ctctacgaac aggaacgtga tagtaaggcg tatatagcgg ataaggaggc
      481 ttcaaactct aaagtccaaa aaggtagtcg taacctatat gtgtaaatca cgagagtaat
      541 tgaattcgga ctaaggtttg tgtgaaaaag ataaatcttt ctagagtcta aagactctgc
      601 gtcagatttc ctattttcac tgtaaccttt taacgtcctc atatcttgta taaacgagga
      661 aagatgtacg acttatcccg tgaggtttca tgagcgctga aagcgtagta acaacgaatc
      721 atgagaagtc agccgagccc atagtagtga ggaaacttct gtagtggaag tggagcgaag
      781 gggtgaatac tcaaacagtc tggggagaga ctgtttgagg tctgtcgcta gaaagagaaa
      841 acgacagatc gaagtaatcc tacttcactt gtgtctgtaa aatgagtggt ctgatagaac
      901 tggactttga cacgtaatga ctagatgaag gttcaccaat ataagatgtc cctcaggcac
      961 caaaacaaga aaggaatacg cacatgtcaa aactgctaga taagatatta tcacgcgaaa
     1021 atatgctgga agcctacaat caagtaaaat ccaataaagg ctcagctggg attgatggaa
     1081 tgactatcga agagatggat aattatctca gacaaaactg gcgcttgact aaggaactga
     1141 taaaacagag aaaatataag cctcaaccaa ttcttaaagt tgagatacct aaaccagacg
     1201 gaggcatccg tcaactagga atttcaacag ttatggatag aatgattcaa caggccattg
     1261 tccaagtcat gagccccatt tgtgaacccc atttctcaga tacgagttat ggtttcagac
     1321 caaataggtc atgtgaaaaa gccatcatga agctcttaga atacttaaat gacggctatg
     1381 agtagatagt ggatatagac ctagagaaat ttttcgatac agttcctcaa gatagattga
     1441 tgtccttagt acataacatt atcgaagacg gagatacgga atccttgatt cgtaagtatc
     1501 ttcattcagg tgttatcatt aatggtcaac gttataaaac actagttggt acaccacagg
     1561 gaggaaattt atctcctctc ttatccaata tcatgcttaa tgaattggac aaggaattag
     1621 aaaagagggg acttcgattt gtgcgctacg cagatgattg tgtgatacgg tcggaagcga
     1681 ggcagccgct aagcgtgtga tgtattcagt cagtcgtttt attgagaaac ggctaggttt
     1741 gaaagtaaac atgaccaaga ctaagattac cagaccaaga gagttgaaat atctaggttt
     1801 tgggttctgg aaatcatcag atggttggaa aagccgtcca catcaagata gtgttcggag
     1861 atttaagttt aaattgaaga aactaacaca gaggaaatgg agtatagacc taacaagacg
     1921 tattgagcaa ctgaatttgt ctattcgagg atggataaac tatttctcat tgggaaatat
     1981 gaaaagtata gtcgccagca tagatgagcg cttgcgtact cgcctacgag tgattatctg
     2041 gaagcaatgg aagaagaaat cgagacgatt atggggattg cttaagttag gagttcctaa
     2101 atggatagca gataaggtat ctggctgggg cgaccattat caattagtag ctcagaagtc
     2161 ggtacttaaa cgtgctatat caaaaccagt ccttgaaaaa cgtggactgg tttcgtgttt
     2221 ggattattac cttgaacgac atgcgttaaa agttagttga accgccgtat gccgaacggc
     2281 acgtacggtg gtgtgagagg ggctagagat tatcccctac tcgattaact cccctgaaat
     2341 ttattttaat tatgcaaatt tcacgtattt ttgatgctga gacgacgatc ctgggaactt
     2401 ttcagatatt tttttgacta tctaaatcta tcattagaaa agcttagagc gccaaaggat
     2461 ttgagcgttt ttctgatttt taagactttt tcccagtctc tttttcgatt gaagatgtaa
     2521 ttattctact aactaactaa cttcttagta ctagccaaca acgataatca taattcctcc
     2581 taaaattagg aataataaag gcaatagttt ttgttttttc atgtaaaaaa cctc
//
LOCUS       DAVMHG010000175         2539 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVMHG010000175 DAVMHG010000000
VERSION     DAVMHG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.177"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..2322
                     /gene="pulA"
                     /locus_tag="VYR08_001975"
     CDS             43..2322
                     /gene="pulA"
                     /locus_tag="VYR08_001975"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV3467445.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGCNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
BASE COUNT          795 a          475 c          535 g          734 t
ORIGIN      
        1 ttattagtga aatagaaaca aagtaaaaag gaaaaataaa aaatgtataa ctaccctatg
       61 cgcattcatt accatcgtaa gaatggagaa tatgacactt gctcctttgt aaaaagtcag
      121 gatcaacgaa ttgatttgct aacctacaaa gaagattatt ttggagcttt atttagcttt
      181 gaacacccaa gttcacatgt tatagaaagc ttgaattttg tggttcatac aggtcaaact
      241 agtaaagaat attctatccg ttttaatcac tatcccctat taacagaggt ctggattttg
      301 gaaggcgatg ataggattta ttactctgaa aatcctgcta ttgccagtcc tttctataaa
      361 aatcaaaatc cttttgcctt tgataaggcc attaacagtg ctagttttga ccatcattgg
      421 ggttaccaag gagaattggg ttgccgtgta gaggacaatc aggctcattt ttccctctgg
      481 tcacctacag cgacagaagt gcaagttgtc gtttatgaat cagctgctaa tgatgcaccc
      541 gtttggaaga cttttgagat gaaaagaggc aatagctact cttataatca taaggacaat
      601 acaatcggtg tctggagttt ggatgttgag gaagatttgg taggtaaggc ttatcagtat
      661 caagtccaat tccctcatca ccaaacactg acacgtgatc cttatacaat cgcgaccagc
      721 cctgatggca aacgttcagc tattctgagc catgtagaaa agcaagttga aaacttcgag
      781 gttaagcacg gttcggaggc tacttggcgc ttggaaaatc catgtaaggc agttatctgt
      841 gaaatgcata ttcgtgattt gactaaatca cctacatcgg gtgtagatga acatcttcga
      901 ggaactttct tgggtgctgc tcaggctgga acagttaacc aatacggcca gtcaactgct
      961 tttgattaca tcaagaagct gggctgcaat tatgttcaat tgcaaccaat tgcagaccgt
     1021 cataaagaat acgatgagga tggaaatgta acctacaact ggggttatga tccacaaaac
     1081 tataacgcgc cagaaactag tttttcaact aatccagatg atccagctca ggtcattcgt
     1141 gatttgaagg tgatggttca agcttatcac gatgcgggta ttggagtcat tatggatgta
     1201 gtctataacc ataccttctc agttgttgat gcaccattcc aaacaacagt ccctgattac
     1261 tattatcgta tgaatccaga tggtaccttc cagaatggaa cgggtgttgg aaatgaaaca
     1321 gccagtgaac acgaaatgtt tcgcaagtat atgattgatt ctcttctata ctgggtgcag
     1381 gaatataata ttgacggctt ccgttttgac ttgatgggga ttcatgatgt caagaccatg
     1441 cagatgattc gtcaaagctt ggatgaaatc gactccaaca ttatcctcta tggagaagga
     1501 tgggatatgg gaacaggtct tgccccttat gataaggcca agaaggacaa tgcctaccag
     1561 atgccaaata ttggtttctt taatgacaat cagcgcgatg ctgtcaaagg gggagaagtt
     1621 tatggtgcta tcaagtcagg ttttgtcagt ggtgctgcga cagagccaat tctagctaaa
     1681 gcaatcctag gaagtcgtga attaggaagc tatacacatc caaatcaggt gcttaactat
     1741 gtagaagccc atgacaatta caatcttcac gatttattgg caacccttca tccagaccaa
     1801 agttcagagc aaatcatgcg caaggtcgaa actgccacag ccatgaatct gctcatgcag
     1861 gggatggcct ttatggaaat cggtcaagaa tttggtcgta ccaaactggt tgcgactggt
     1921 gaaaatggtg agttgaccca tgatgataga gagcgtgcga tgaatagcta taatgctcct
     1981 gacagtgtga accaagtgaa ctggaacttg attaatgagc gtcaagacag tattgagttt
     2041 atccgtcaag tcatccgatt gaagacaaaa actggtgcct tttcttactc tagctatgat
     2101 gaaatttacc atcatgtctt tgtgcattct gcgattgaac atagcggcta ccttatctat
     2161 gaagttcacg gcaaagaaca cctcttggtg gttgtgaatg ctaaatcaga accctatcaa
     2221 tttgaaaatg caggaaattt agctatgttg gtaaccaaca gtcgctcaaa agaagataat
     2281 gttttaaatg atattagtct agctgtcttg agtgttttat aaataccatc tatctcgcta
     2341 tttttacaca gacaaattcc cagttatgag tattagtaaa aaatccattc attgaaattg
     2401 aaagaataga aagaacctgg ctaacagctc acttgtatag aaaatttgat aaatattttc
     2461 acaaaacaag acctcctatc aaagtttttg aacacttgat aggaggtctt tttattatga
     2521 agacttttac ctagacttt
//
LOCUS       DAVMHG010000176         2507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVMHG010000176 DAVMHG010000000
VERSION     DAVMHG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.178"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..2492
                     /locus_tag="VYR08_001976"
     rRNA            <1..2492
                     /locus_tag="VYR08_001976"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          701 a          515 c          743 g          548 t
ORIGIN      
        1 gggacacgtg aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct
       61 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
      121 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
      181 tgccttttgt agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg
      241 gagccgtagg gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac
      301 catgtgacct acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca
      361 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
      421 gagatagctg gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg
      481 aggtagagca ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg
      541 aatgccaatg aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg
      601 gaaacagccc agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg
      661 ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa
      721 tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga
      781 agctgtggat acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc
      841 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg
      901 agaatcctgt ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
      961 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     1021 ctagagtatg tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt
     1081 ctgtctaagc agtgaggtgt gaattgagtc aaatgcttaa ttctataaca ttgagctgtg
     1141 atggggagcg aagtttagta gcgaagttag tgacgtcaca ctgccaagaa aagcttctag
     1201 cgtttaaaca tactctaccc gtaccgcaaa ccgacacagg tagtcgaggc gagtagcctc
     1261 aggtgagcga gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga
     1321 aggggtgctg acttaaagtc agccgcagtg aataggccca agcaactgtt tatcaaaaac
     1381 acagctctct gctaaatcgt aagatgatgt atagggggtg acgcctgccc ggtgctggaa
     1441 ggttaagagg agtgcttagc gtaagcgaag gtatgaattg aagccccagt aaacggcggc
     1501 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg
     1561 aaaggcgtaa tgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt
     1621 gaagatgcag gttacccgcg acaggacgga aagaccccat ggagctttac tgcagtttga
     1681 tattgagtgt ctgtaccaca tgtacaggat aggtaggagt ctaagagatc gggacgccag
     1741 tttcgaagga gacgctgttg ggatactacc cttgtgttat ggccactcta acccagatag
     1801 gtgatcccta tcggagacag tgtctgacgg gcagtttgac tggggcggtc gcctcctaaa
     1861 aggtaacgga ggcgcccaaa ggttccctca gaatggttgg aaatcattcg cagagtgtaa
     1921 aggtataagg gagcttgact gcgagagcta caactcgagc agggacgaaa gtcgggctta
     1981 gtgatccggt ggttccgtat ggaagggcca tcgctcaacg gataaaagct accctgggga
     2041 taacaggctt atctccccca agagttcaca tcgacgggga ggtttggcac ctcgatgtcg
     2101 gctcgtcgca tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc
     2161 ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt
     2221 aggaaatttg agaggatctg ctcctagtac gagaggacca gagtggactt accgctggtg
     2281 taccagttgt cttgccaaag gcatcgctgg gtagctatgt agggaaggga taaacgctga
     2341 aagcatctaa gtgtgaaacc cacctcaaga tgagatttcc catgattata tatcagtaag
     2401 agccctgaga gatgatcagg tagataggtt agaagtggaa gtgtggcgac acatgtagcg
     2461 gactaatact aatagctcga ggacttatcc aaagtaactg agaatat
//
LOCUS       DAVMHG010000177         2494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVMHG010000177 DAVMHG010000000
VERSION     DAVMHG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.179"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(117..1343)
                     /locus_tag="VYR08_001977"
     CDS             complement(117..1343)
                     /locus_tag="VYR08_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV3467446.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1415..2080)
                     /locus_tag="VYR08_001978"
     CDS             complement(1415..2080)
                     /locus_tag="VYR08_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3467447.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2148..2345)
                     /locus_tag="VYR08_001979"
     CDS             complement(2148..2345)
                     /locus_tag="VYR08_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV3467448.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
BASE COUNT          928 a          480 c          439 g          647 t
ORIGIN      
        1 tattttagat tcagtctatt ataacacatt cagaaaagag aaaaaagtct gttgattttg
       61 accatcataa aaagactggc aatccagtct caaacatata ttatagaaat tctccactaa
      121 atactttcac gaatattcag aagcataaca aaggcaacta gaagaaatag caataaaaca
      181 aagctaactg ccagagttcc aaagctagta gcaatggtta ccaaagctat tgtaaataag
      241 ctaggtaaaa caaccgtaat ggcaccgata gaggattgaa ctgctcccat tgactcctca
      301 ggtatttgtt taaagacgag ttcttgcaat ctaggagaga gaacacctgc gaaaaaggca
      361 tccaaggtac taaagatgag aatccagtca aaacgaactg tggcaaatcc tactagaaga
      421 agcaactgga tgacaagtga ggcatagaga gctgttttta tggaaatggt atgttgcaga
      481 tagccactta caaggcttcc gacaatcagg gctgacagtt caaaagtgga tagcaaggca
      541 agagattgac ctgtttgtaa attcaaaaag ggttgattcc tcaaaaatag agtagagaca
      601 ggaactgtaa catttatcac tgcttgacta gtagagataa taaacaaaac caagagcacc
      661 ttattcatat tccatatcaa tttcgatgat tggagcaaat gctggcaaaa ggattttaca
      721 gagagtcctt cttgatagtt aatcgttttt tctactttca agaggtcagt ttttatgaag
      781 aggataccta aaaatgcgat taaaaaggta agagcgttca gcaaggaaat gaactggatg
      841 gagagaatcc ctagcaagac tcctcctaga atattactga ttgttttcac taaactaaca
      901 gttgactgtt taaatccaat agcttctgcc agatggtctt gcccaataat tctaatgaaa
      961 atcggagtga gcatggctcc tgaaaagtaa ctcaatgtgt cagacaagag gttaagcaga
     1021 caaataaatg ctactagcaa caaggagaaa gactgccctg aaagtgataa agaaacgata
     1081 gagtaaagca aaaattttgc aaaactaatg actgtgtatt tcaagacacg atgatgttga
     1141 aaatctgcca aaactcccag aaagatttgt aaaatttggg gaagggtttc tgaaatcgta
     1201 atgagtaaaa tcgccaaagg tgcaaaagac gcatctgcca cataattcag aaaggccaga
     1261 taaaaaatcg tatccccaag cgttgaaatc cactggttga tagttaattg cctaaaatct
     1321 ctattttgaa gaaatacttt catcacaact ccttcttaag ttcaaatggg aatctttccc
     1381 caaggataga ccgcgatact actaacaacc aaaattacag taacatcaaa agctgaccaa
     1441 tgccattgta gactatatgc agtccaatag gccaataaat tgactttgtc attctaaata
     1501 agactgcaaa tataagacct ccacccatat agaagacaaa gtctgtcaag acccaaccgt
     1561 gattactaat gtgcgagacc ccaaataaaa cagcggaacc aagtacatct agcccccatt
     1621 tctttccttt ttccagagca gtcatcacta atccacgata aatcatgtct tcaaaaatgg
     1681 gacctgcaat cacaggataa aaaaaataca tcaaaaatgc cgtagctcct gtaaaagtag
     1741 gagcagcatg ttgataagaa atttcatttc gagtaggtgg gaaaagaaaa aaggtgacaa
     1801 aattccaagc aacaaaagca agcagagcta ggaaggaata gaaaagatag gatcctttaa
     1861 actttctact attgattttc tgccatttcc ccgaccaaat catagcaata agagcaaata
     1921 aaaccacaag aaaattcaac atcatatccg acagataata ggcaaagtca gatagcccag
     1981 caacaaggtc gctgcgtaaa actaaaacac tgaacttctg gtcagcaata actagtagaa
     2041 aaactataat aaagtagcgg tgtgagatta tctttttcat tctattttct cctatactat
     2101 gaaataaaga cagtccctac ccaagaaaaa ggcaaggact ctgaatatca ataatggcga
     2161 cgattaacag tggaagaatc atggttaaaa tatttcttcc agaaattagt gattgaccaa
     2221 gacgttccta aacctcccca agcaccggct gggataacag cattattccc aaattcccct
     2281 ccataaactg tctccaactc agcttctgtc aattccattg tttctgcaaa ttgtaaattt
     2341 aacatctttt acactccttc aattatcttc atttgtaaac cacttctgcg acctaggatt
     2401 cgcttcaagt gctttacaag tacagtataa cacgaaaatt ggcttatttt aaaaaatcgc
     2461 atatttgata ttttttctta tagaaatttc ttat
//
LOCUS       DAVMHG010000178         2460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVMHG010000178 DAVMHG010000000
VERSION     DAVMHG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.180"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..1672
                     /gene="mltG"
                     /locus_tag="VYR08_001980"
     CDS             17..1672
                     /gene="mltG"
                     /locus_tag="VYR08_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV3467449.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            1743..2225
                     /gene="greA"
                     /locus_tag="VYR08_001981"
     CDS             1743..2225
                     /gene="greA"
                     /locus_tag="VYR08_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV3467450.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT          822 a          460 c          540 g          638 t
ORIGIN      
        1 caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa gctttaaaga
       61 gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga aagaagatga
      121 ggcagtagtt cgcactcctg caaatgaacc ttcagctgaa gaactcatgg ctgattcctt
      181 gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc caagtcaagg
      241 tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc caagtcatcc
      301 aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag gtccaggtcc
      361 tgttagacct aagaaacttg aaagagaata caatgaaacc ccaacaaggg tagctgtttc
      421 ctatacgacg gcagagaaaa aagcagaaca agcaggtcca gaaacaccta cgcctgctac
      481 agaaacagtg gatatcatca gagatacatc acgtcgtagc cgtagagaag gagcaaaacc
      541 cgttaagcct aagaaagaga agaagtcaca tgtgaaagct tttgtgattt cattccttgt
      601 attccttgcc ttgctctcag caggtggtta ctttggttac cagtacgtgc tagattcctt
      661 attacctatc gatgctaatt ctaagaaata tgtgacggtt ggaattccag aaggttcaaa
      721 cgttcaagaa atcggtacga cgcttgaaaa agctggtttg gtaaagcatg gtctgatttt
      781 tagtttttat gccaagtata aaaattatac cgacttgaaa gcaggttact acaatttgca
      841 aaagagtatg agtacagaag acttactcaa agagttgcaa aaaggtggaa cagatgaacc
      901 gcaagaacct gtacttgcga ctttgacaat tccagaaggt tataccttgg atcagattgc
      961 tcaaactgtg ggtcaattgc aaggtgactt caaagagtct ttgacagcgg aggctttctt
     1021 ggctaaagtt caagatgaga cgtttatcag tcaagcagta gcgaaatatc ctactttact
     1081 ggaaagtttg cctgtaaaag acagcggtgc gcgttatcgt ttggaaggat accttttccc
     1141 agctacatac tctatcaagg aaagcacaac tattgagagc ttgattgatg agatgttagc
     1201 tgctatggat aagaacctat ctccttacta tagtactatc aaatctaaaa acttgactgt
     1261 caatgagttg ttgaccattg cttccttggt cgaaaaagaa ggtgccaaga cagaagatcg
     1321 taagctcatt gcaggtgtat tctacaatcg tttgaatcgt gatatgccac ttcaaagtaa
     1381 tattgcaatc ttgtatgccc aaggaaaact ggggcaaaat atcagtctag ctgaggatgt
     1441 tgcgattgat accaacattg attcacctta taatgtttat aaaaatgtag gtctcatgcc
     1501 tggtccagtc gatagtccaa gtctggatgc gattgagtca agcatcaatc aaactaagag
     1561 cgataacctc tactttgtag cagatgtcac agaaggcaag gtctactatg ctaacaatca
     1621 agaagaccac gaccgcaatg tcgctgaaca tgtcaacagc aaattaaact aaacaaacta
     1681 aaattatgtg atacttcaca taattttctt tagaaaatat tatcagaaga aagttgagaa
     1741 aaatggcaga aaaaacatat cctatgaccc ttgaggaaaa ggaaaaactt gaaaaagaat
     1801 tagaagaatt gaaattggtt cgtcgaccag aagtggtaga acgcattaag attgcccgtt
     1861 catacggtga cctttcagaa aacagtgagt acgaagcagc taaggatgaa caagcctttg
     1921 tcgaaggaca aatctctagc ttagaaacaa aaatccgcta tgctgaaatc gtcaatagcg
     1981 acgcagttgc ccaggacgaa gtagcgattg gtaaaacagt caccatccaa gaaattggtg
     2041 aggacgaaga agaagtttat attatcgtag gttcagctgg tgcggatgcc tttgcaggta
     2101 aggtttcaaa tgaaagccca attgggcagg ccttgattgg caagaaaaca ggtgatacag
     2161 caaccattga aacgcctgtt ggtagctatg atgtaaaaat cttgaaggtt gaaaaaacag
     2221 cctaaaaaca gaaaaaggag tggggaggcg atgtgcttca ctcactcctt tttccatttt
     2281 gctactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttgccgtat gtatggttac
     2341 tgacttcgtc agtttcatct acaacctcaa aacagtgttt tgagcaacct gcggctagtt
     2401 tcctagtttg ctctttgatt tttattgagt attagatgta tagtgcattg aagttaaaat
//
LOCUS       DAVMHG010000179         2432 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVMHG010000179 DAVMHG010000000
VERSION     DAVMHG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.181"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            37..534
                     /locus_tag="VYR08_001982"
     CDS             37..534
                     /locus_tag="VYR08_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV3467451.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            534..2189
                     /gene="dnaX"
                     /locus_tag="VYR08_001983"
     CDS             534..2189
                     /gene="dnaX"
                     /locus_tag="VYR08_001983"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV3467452.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2217..2411
                     /locus_tag="VYR08_001984"
     CDS             2217..2411
                     /locus_tag="VYR08_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV3467453.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
BASE COUNT          714 a          451 c          539 g          728 t
ORIGIN      
        1 cttttgtctc tgctcctttt ttgatataat agttctatgt taaaatcaga aaaacaatca
       61 cgttatcaaa tgttaaatga agaattgtcc ttcctattgg aaggcgaaac caatgttttg
      121 gctaatcttt ccaacgccag tgctctcata aaatcacgtt ttcctaatac cgtatttgca
      181 ggcttttatt tgttcgatgg aaaggaattg gttttaggcc ccttccaagg aggtgtttcc
      241 tgcatccgta ttgcactagg caagggtgtt tgtggtgagg cagctcactt tcaggaaact
      301 gttattgttg gagatgtgac gacctatctc aactatattt cttgtgatag tctagctaaa
      361 agtgaaattg tggtgccgat gatgaagaat ggtcagttac ttggagttct ggatctggat
      421 tcttcagaga ttgaggatta cgatgctatg gatcgagatt atttggaaca atttgtcgct
      481 attttgcttg aaaagacagc atgggacttt acgatgtttg aggaaaaatc ttaatgtatc
      541 aagcacttta tcgaaaatat agaagtcaaa acttctccca gttagttggt caagaagttg
      601 tggctaagac tcttaaacaa gcggtggagc aagagaaaat aagtcacgct tatctttttt
      661 ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat ctttgccaag gctatgaact
      721 gtcccaatca agtgggtggc gaaccttgca ataactgcta tatttgtcaa gcagtgacgg
      781 acggtagttt agaagatgtc attgaaatgg atgcagcttc taataatggg gtagatgaaa
      841 ttcgcgaaat tcgtgataaa tctacctatg cgcctagcct tgctcgttat aaggtttata
      901 tcatagatga ggttcacatg ctgtctacag gggcttttaa tgccctccta aagacgctgg
      961 aagaaccaac acagaatgta gtctttattt tggccactac tgaattgcac aagattcctg
     1021 ctactattct atcccgtgtg caacgttttg agtttaaatc aattaagaca caggatatta
     1081 aggaacatat tcactatatc ttagaaaaag aaaatatcag ttctgaacca gaggctgtgg
     1141 aaatcattgc cagacgggcg gaaggtggaa tgcgggacgc cttgtctatt ttggatcaag
     1201 ccctgagttt gacacaggga aatgagctga cgactgctat ctctgaagaa attactggca
     1261 ccattagcct atcagccttg gatgattatg tggcggcctt gtctcaacag gatgttccca
     1321 aagctttgtc ttgcttgaat cttctttttg acaatggtaa gagcatgact cgttttgtga
     1381 ccgatctttt gcactattta agagacttgt taattgttca aacaggggga gaaaatactc
     1441 atcatagttc agtctttgta gaaaatttgg cacttcctca aaaaaatctg tttgaaatga
     1501 ttcgcttagc aacagtgaat ttagcagata ttaagtctag tttgcagccc aagatttatg
     1561 ctgaaatgat gaccgtccgt ttggcggaaa tcaagcccga accagctcta tcaggagcgg
     1621 ttgaaaatga aattgctacg ctgagacagg aagttgcccg tctcaaacaa gagctttcta
     1681 atgcaggtgc ggttcctaaa caagttgcac cagctcctag tcgaccagct acgggcaaaa
     1741 cagtctatcg tgtcgatcgc aataaagtgc aatctatctt acaagaggcc gtcgaaaatc
     1801 ctgatttagc acgtcaaaat ctaattcgtt tgcagaatgc ctggggagag gtaattgaaa
     1861 gtctaggtgg gccggacaag gctctgctag ttggttctca accggttgct gccaatgaac
     1921 accatgctat tcttgctttt gagtctaact tcaatgctgg tcaaactatg aaacgagaca
     1981 atctcaatac catgtttggt aatatcctca gtcaggcggc aggtttttca cctgagattt
     2041 tagctatttc catggaggaa tggaaagaag ttcgcgcagc cttttcagcc aaagccaaat
     2101 cttctcaaac tgaaaaagaa gtagaagaaa gcctgattcc agaaggattt gaatttttgg
     2161 ctgataaagt gaaggtagag gaagactaaa gaaagatttc atgatacaat aagtttatga
     2221 ataaacaaca atttattatt atggcgctat ttacagctgc tgagacctat tttttcaatg
     2281 aagcctggat gactggccgc tatattatgg cagccttttg ggcaatttta ctctttagaa
     2341 atttccgagt cagttatgtg atgggcaaaa tcgttgatgt catcgatcag cattttaata
     2401 ggaaagacta gccctcagct tccagacaaa at
//
LOCUS       DAVMHG010000180         2409 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVMHG010000180 DAVMHG010000000
VERSION     DAVMHG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.182"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..252
                     /locus_tag="VYR08_001985"
     CDS             <1..252
                     /locus_tag="VYR08_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467454.1"
                     /translation="KKLENKIKKLERKEKGNAEMSRLLQEMIGKEPIITGVYIGPDNW
                     EVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            262..468
                     /locus_tag="VYR08_001986"
     CDS             262..468
                     /locus_tag="VYR08_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467455.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            468..665
                     /locus_tag="VYR08_001987"
     CDS             468..665
                     /locus_tag="VYR08_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467456.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            668..1402
                     /locus_tag="VYR08_001988"
     CDS             668..1402
                     /locus_tag="VYR08_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV3467457.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1414..2055
                     /locus_tag="VYR08_001989"
     CDS             1414..2055
                     /locus_tag="VYR08_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3467458.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2073..2303
                     /locus_tag="VYR08_001990"
     CDS             2073..2303
                     /locus_tag="VYR08_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467459.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          686 a          411 c          501 g          811 t
ORIGIN      
        1 aagaaactag agaataaaat caaaaaactt gagcgaaaag agaaaggaaa cgcagaaatg
       61 tcgagattat tgcaagaaat gattggaaag gaaccaatta taacgggagt gtatattggg
      121 ccagataact gggaagttgt ggatgttgat gaggaatggg taaagctacg acgtgtagat
      181 aatacgggaa aagaaaaatt caagttgcaa cgtattgaag atatccaaac cgttgaattt
      241 gacggagagt aggtgtgtaa cgtgcaacta aaaaatcgtc taaaagagct tcgagctcgc
      301 gatggtctca atcaaaccga cctagccaaa ctggcagggg tttccagaca gaccattagc
      361 ctactagaac gggatgagta caccccatcc attatcattg ccctgaaaat atctcaaatt
      421 ttcaacgaaa cagtcgaatc ggtatttcgt ttggaggagg acgagtgatg aacaagtata
      481 aagtgattta ttatgtagtg gtcatagctt tattagtcag cgtatttcta ctgattggga
      541 tggacctaag ctggtttaat ccctatcaaa gcgaccaatt tgtttgggtc tactttgctc
      601 tcatcccagt aattgaatgg attgaaaaga aatccaaaaa tctagcaagt gaaaaaggag
      661 aatgaatatg aaaaagaaac gtagattgtt atttttgatg tctattgtct tgggggggtt
      721 cttgggcatg tttgtaggga tgtttaaggc acgtgtcgaa tcccacgaaa ttattttaga
      781 tgtaaaagcc ttgatgccat ggatatcagc tatttgttta ctgataggtt tcattagtat
      841 gtttttgact ttcaatttct taaagaaaag cagaaaattt cattccttgt atcaagagga
      901 aatggatgac gatctgaatg aaacctatta tgtgcaaatg tatcggaatc ttgagtttgg
      961 aaccattgct tttaatatta caggtgtagc gattccattg gctattttta tttcattaag
     1021 tgaggtgatt atattgcata caaaccctca aacatttttc ctttctttct tactctttgt
     1081 ggtattctta gtcgctcaaa aatctctttt taaaaccatt gcgattgttc gtcagtttga
     1141 tttggaattt ttcgctacac caaaggatgt cttgaactat ataaattctt atgatgaagg
     1201 ggagcgtcag gctaatttgg aacagagttt tcgaatttta ttccaattac accaatatgt
     1261 cttaccagcc ttatatattt ttcttattat catttctttc ttgacaggag agattcagtt
     1321 actagctttc ttgcttgtag gagccatcca tgtttatatc aatgtgatgc agttacctat
     1381 ggtaaaacgt tatttcaaat aaaggagtta tctatgaaac tacttaaaaa ccttggctgg
     1441 tttcttctag cccttctatc ctttttcttt atctatggct ttattcaggg gctcgcgact
     1501 gcgtcgcttg ctttaggcgc ttcaccctat gctgttactc tactctatgt ggtcttggct
     1561 ggagtatatg tgtacggcat ttacaaatgg tatcagaaag ctcctgttta tattgagaag
     1621 agcggcttta accgatttat ttggcttcct gccttggttt ggttcttatc tctggtcgtt
     1681 caattcttct tgccagatga tccttcagta aatcaacaaa tagcgacaga cttgaccttg
     1741 tctcaaccac ttttctcatt ctttgctgtg gttatttttg ctcctttgac ggaagagatt
     1801 gtttttagag ggatgttagc acgctatctc tttcctaagc aggacaatag taaacgaacc
     1861 ctgatttttc ttctggtatc tagtcttcta tttgccttga ttcattttcc aggtgatgtg
     1921 caacaatttt ttgtctattt tagccttggt tttagtttgg gtttggctta cattagcaga
     1981 aaaggtctgg tctacagtat ttctcttcac gctttgaata atttagtcgg ctttttgatg
     2041 attctcatgc tataatagag tcaggaggtc acatgaaacg agtaatttta ttagcagtga
     2101 ttcaggcagt cgttctattt ttcatcattg gagcgctagc ttatgccttc aaaggcgatt
     2161 tcttctataa ctatctagca gttgtctttg ctcctattgc aggtgtactg cgttttggga
     2221 cggcttacat aacggaaatt gtcttgcctc gaaaggcagc cgaaatcgct gaaaagcgta
     2281 aagcaggcaa aaattcaaaa taaaagagtc cggtgaagtt catcggattt ttgtattata
     2341 ctcaatgaaa atcaaagagc aaactaggaa gctaaccgca ggttgctcaa aacactgttt
     2401 tgaggttgt
//
LOCUS       DAVMHG010000181         2336 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVMHG010000181 DAVMHG010000000
VERSION     DAVMHG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.183"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>2336
                     /gene="mfd"
                     /locus_tag="VYR08_001991"
     CDS             <1..>2336
                     /gene="mfd"
                     /locus_tag="VYR08_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV3467460.1"
                     /translation="YPMQEFFNQFSFLKEEIERYKKMDYTIILQSSNSMGSKTLEDML
                     EEYQIKLDSRDKTSICKESVNLIEGNLRHGFHFVDEKILLITEHEIFQKKLKRRFRRQ
                     HVSNAERLKDYNELEKGDYVVHHIHGIGQYLGIETIEIKGIHRDYVSVQYQNGDQISI
                     PVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAKQKVKNQVEDIADDLIKLYSERSQLK
                     GFAFSADDDDQDAFDDAFPYVETDDQLRSIEEIKRDMQASQPMDRLLVGDVGFGKTEV
                     AMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFKERFQNFAVNVDVLSRFRSKKEQTAT
                     LEKLKNGQVDILIGTHRVLSKDVVFADLGLMIIDEEQRFGVKHKETLKELKKQVDVLT
                     LTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQTYVLEKNDSVIRDAVLREMERGGQ
                     VYYLYNKVDTIVQKVSELQELIPEASIGYVHGRMSEVQLENTLLDFIEGQYDILVTTT
                     IIETGVDIPNANTLFIENADHMGLSTLYQLRGRVGRSNRIAYAYLMYRPEKSISEVSE
                     KRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLGKSQSGFIDSVGFELYSQLLEEAIAK
                     RNGNANANTRTKGNAELILQIDAYLPDTYISDQRHKIEIYKKIRQIDNRVNYEELQEE
                     LIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRVERKDNKITIQFEKVTQRLFLAQDYF
                     KALSVTNLKAGIAENKGLMELVFDVQNKKDYEILEGLL"
BASE COUNT          765 a          350 c          505 g          716 t
ORIGIN      
        1 tatcctatgc aggaattttt caatcagttt tcttttctaa aagaagaaat tgaacgatat
       61 aaaaaaatgg attacaccat tattctgcag tctagcaatt caatgggaag taaaacattg
      121 gaggatatgt tagaggaata tcagattaaa ttggattcta gagataagac aagtatctgt
      181 aaagaatctg taaacttaat agagggtaat ctcagacatg gttttcattt tgtagatgaa
      241 aagattttat tgataactga acatgagatt tttcaaaaga aattaaagcg tcgttttcga
      301 agacaacatg tttcaaatgc agagagatta aaagattaca atgaacttga aaaaggggac
      361 tatgttgtcc atcatatcca tgggattggt caatatctag gaattgaaac cattgaaatc
      421 aagggaattc atcgcgatta tgtcagtgtc cagtaccaaa atggtgatca aatttctatt
      481 cccgtggaac agattcatct actgtccaaa tatatttcaa gtgatggtaa agctccaaaa
      541 ctcaataaat taaatgacgg tcattttaaa aaggccaagc aaaaggttaa gaaccaggta
      601 gaggatatag ctgatgattt aatcaaactc tactctgaac gtagtcagtt gaagggtttt
      661 gctttctcag ctgatgatga tgatcaagat gcctttgatg atgctttccc ttatgttgaa
      721 acggatgatc aacttcgtag tattgaggaa atcaagaggg atatgcaggc ttctcagcca
      781 atggatcgac ttttagttgg ggatgttggt tttggaaaga ctgaagttgc tatgcgtgca
      841 gcctttaaag cagtcaatga tcacaaacag gttgtcattc tagttccgac gacggtttta
      901 gcgcaacagc actatacgaa ttttaaggaa cgattccaaa attttgcagt taatgttgat
      961 gtgttgagtc gctttagaag taaaaaagag cagactgcaa cacttgaaaa attgaaaaac
     1021 ggtcaagtcg atattttgat tggaacacat cgtgttttgt caaaagatgt tgtgtttgct
     1081 gatttgggct tgatgattat tgatgaggaa cagcgatttg gtgtcaagca taaggaaact
     1141 ttgaaagaac tgaagaaaca agtggatgtc ctaaccttga ccgctacgcc aatccctcgt
     1201 accctccata tgtctatgct gggaatcaga gatttgtctg ttattgaaac tccgccgact
     1261 aatcgctatc ctgttcagac ctatgttttg gaaaagaatg atagtgtcat tcgtgatgct
     1321 gtcttgcgtg aaatggagcg tggaggtcaa gtttactatc tttacaacaa agttgacaca
     1381 attgttcaga aggtttcaga attacaggag ttgattccgg aggcttcgat tggatatgtt
     1441 catggtcgaa tgagtgaagt ccagttggaa aatactctat tagactttat tgagggacaa
     1501 tacgatatct tggtgacgac tactattatt gagacagggg tggacattcc aaatgctaat
     1561 actttattta ttgaaaatgc ggaccatatg ggcttgtcaa ccttatatca gttaagagga
     1621 agagtcggtc gtagtaatcg tattgcttat gcttatctca tgtatcgtcc agaaaaatca
     1681 atcagtgaag tctctgaaaa gagattagaa gcgattaaag gatttacaga attgggctct
     1741 ggctttaaga ttgcaatgcg agatctttcg attcgtggag caggaaatct tttaggaaaa
     1801 tcccagtctg gtttcattga ttctgttggt tttgaattgt attcgcagtt attagaggaa
     1861 gctattgcta aacgaaacgg taatgctaac gctaacacaa gaaccaaagg gaatgctgag
     1921 ttgattttgc aaattgatgc ctatcttcct gatacttata tttctgatca acgacataag
     1981 attgaaattt acaagaaaat tcgtcaaatt gacaaccgtg tcaattatga agagttacaa
     2041 gaggagttga tagaccgttt tggagaatac ccagatgtag tagcctatct tttagagatt
     2101 ggtttggtca aatcatactt ggacaaggtc tttgttcaac gtgtggaaag aaaagataat
     2161 aaaattacaa ttcaatttga aaaagtcact caacgactgt ttttagctca agattatttt
     2221 aaagctttat ccgtaacgaa cttaaaagca ggcatcgctg agaataaggg attaatggag
     2281 cttgtatttg atgtccaaaa taagaaagat tatgaaattt tagaaggtct gctgat
//
LOCUS       DAVMHG010000182         2285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVMHG010000182 DAVMHG010000000
VERSION     DAVMHG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.184"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..916
                     /locus_tag="VYR08_001992"
     CDS             104..916
                     /locus_tag="VYR08_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV3467461.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            1255..2274
                     /gene="adhP"
                     /locus_tag="VYR08_001993"
     CDS             1255..2274
                     /gene="adhP"
                     /locus_tag="VYR08_001993"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV3467462.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT          678 a          489 c          485 g          633 t
ORIGIN      
        1 aagaaattaa tccaatttca ttttttgttt ggaatctgct tttctgcctg ctttctgtta
       61 taatagaaga catcttatct gaaaacacac tagaaaggtc catatgacga aaaaaattat
      121 tgcagttgac ctagatggaa ccctgctcaa ctcagacagt caaatttctg actttaccaa
      181 aaaaaccatc aaaaaagttg ctgaaaaagg ccatcaggtt attattacga caggtcgccc
      241 ttaccgtatg tcaaaagatt tttaccgtga actgggctta gacactccta tgattaactt
      301 caacggatcc cttactcatt taccagacca agtttgggat tttgaaaagt gtttgactgt
      361 agacaaaaaa tatctgctag atatggttca acgttcagag gacattcaag ccgattttat
      421 cgctggagaa tatcgtaaaa aattctacat tacaaatccc aatgaagaaa ttgccaatcc
      481 caaactattt ggtgtagaag ctttccagcc tgaagatcaa ttccagcctg aattggtgac
      541 caaggaccct aactgtatcc tcttgcagac cagagccagt gacaaatatg ccttggcaaa
      601 agacatgaac gccttctacc agcatcaact ttctatcaat acctggggag gtccgctcaa
      661 tatccttgaa tgtaccccaa aaggtgtcaa caaggccttt gctttggact acttgctcaa
      721 gataatgaat cgtgacaaaa aagatttgat tgcctttggg gatgaacaca atgataccga
      781 aatgctcgct tttgctggga agggttatgc catgaaaaat gccaatccag agctactccc
      841 ttatgcagat gagcaaattt cccttaccaa cgaccaagat ggggttgcca aaaccctaca
      901 agacttattc ttataaccta tactgatact caatgagaat caaagagcaa actaggaagc
      961 tagccgtagg ttgctcaaag cactgctttg aggttgtaga tgaagctgac gtggtttgaa
     1021 gagattttcg aagagtatga ccagcttgca agctggtctt tgtgctcttt tcacagtctc
     1081 atcaaccagt taatcgattg ttctactttt tgtctccaaa accttggaaa aggctttctt
     1141 ttgtgatata cttcacatat aaatacaaga gagctcgtca cactcgactc tgttgacaaa
     1201 aaatcaatcc agtcccgttg tccctgttct cggtcaatat caaggaggat agctatgaaa
     1261 gctgttgttg taaatccaga aagcactggt gttgctattg aagaaaaagt actccgtcca
     1321 cttgaaactg gggaagcact tgtagaagtt gaatactgtg gcgtttgcca caccgacctc
     1381 cacgttgctc atggtgactt tggtcaagtc ccaggacgtg ttcttgggca cgaaggtatc
     1441 ggtatcgtta aagagattgc tccagatgtg aaaagcctta aagtcggtga ccgcgtcagc
     1501 gttgcttggt tctttgaagg atgtggcact tgcgaatact gtacaactgg tcgcgaaacc
     1561 ctttgccgta cagtgaaaaa tgctggctac tcagtagacg gtggtatggc tgaacaatgt
     1621 atcgtaactg ctgactatgc tgtcaaagtt cctgacggac ttgatccagc ccaagcttct
     1681 tctatcacat gtgctggagt aacaacctat aaagctatca aagaagcaaa agttgaacca
     1741 ggccaatggg ttgttcttta cggtgctggt ggacttggta acctcgctgt tcaatacgct
     1801 aaaaaagtat tcaatgctca tgttatcgca gtcgatatca acaatgacaa acttgccctt
     1861 gcaaaagaag taggcgctga cattgtgatt aacggcctcg aagttgaaga tgtagctgga
     1921 ctcattaaag aaaaaactga tggaggagct cattcagctg tcgtaactgc tgtgtctaaa
     1981 gttgccttca accaggctgt tgactccatt cgtgctggtg gtcgcgtcgt cgctgttggt
     2041 cttccttctg aaatgatgga actcagcatc gttaaaacag tcctcgatgg aatccaagtc
     2101 atcggttctc ttgtcggaac tcgtaaagac ttagaagaag ccttccaatt tggtgcagaa
     2161 ggtctggtag tcccagttgt tcaaaaacgt ccagtagaag atgctgttgc cattttcgac
     2221 gaaatggaaa aaggccaaat ccaaggacgt atggtactcg acttcaccca ctaatctaat
     2281 agaaa
//
LOCUS       DAVMHG010000183         2280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVMHG010000183 DAVMHG010000000
VERSION     DAVMHG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.185"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..935
                     /locus_tag="VYR08_001994"
     CDS             <1..935
                     /locus_tag="VYR08_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV3467463.1"
                     /translation="LPTDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFS
                     NKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHST
                     ANIRNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPR
                     IGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERK
                     PKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIFEKNFE
                     LFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            1331..2194
                     /locus_tag="VYR08_001995"
     CDS             1331..2194
                     /locus_tag="VYR08_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV3467464.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLIALFE
                     LRLQQYKKLID"
BASE COUNT          779 a          352 c          431 g          718 t
ORIGIN      
        1 cgcttccgac agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa
       61 cggtatcgaa gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc
      121 cgatcggctt ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc
      181 ctgaattttt gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg
      241 ttggagatga gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc
      301 atagtactgc caatattaga aatgttatgt tagtttctcc tactgaagca gaagcaatta
      361 agcttttttc taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt
      421 ttgctgagag gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa
      481 gaattggaaa tttttataat aatccttctt ttggatttgg aggatattgt cttcccaaag
      541 atactaaaca attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg
      601 atatttcaaa cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa
      661 agatagtagg aatatataaa ttggggatga aatataattc agataattac aaagagtcag
      721 ctattttaag tataattaat gaactattga ttgtcggtat aaaaatttta gtatatgaac
      781 caaatttaaa cgttagtata gataacgtaa tttttgaaaa gaattttgaa ctatttacaa
      841 aacaaagtga tctaattgtt gcaaatagat gggatagggg ccttgaagct tataaagata
      901 aagtttacac aagggggatc tggataagag attaactatt gatattaaaa aggcaataat
      961 ttataaaatt tgtacaaaaa aatgttaaat tttcacattt tcatagtata ctgtttaata
     1021 gaaagtaggt tctttgtaac cgctccatta taaaatcctt tcactcttcc ggtatactat
     1081 taggaaaaac ataactggag gatattttga gctcacacct catcgttcca gtggctgttc
     1141 cccaagctcg tgtactgccc aaaaaagtta gacattctat ttagaacgag gattgagttc
     1201 tgtattgtac aaggctcggt ccttttagag tcagcttaag gctggctttt ttattttttc
     1261 taactatcag aatattttga caaatgaaca caaataatga tataatatgc aaaagctagg
     1321 aggtggtagg atgattgaaa aaatggaatt gggggaattt tacaaggaat tacgtttggc
     1381 gagaaagctc aagcagtcag atgtggcttg tgacgggcta acagcttctc agttgtccaa
     1441 gtttgagcta gggcagtcca tgttgtctgc tgataagctc atattagcca tccaagggat
     1501 caatgtgacc tttgatgagt ttgggcacaa gctcaacaac tatcaagaat ctccacatat
     1561 gcagattggt agaaaggttg tggatcgctt tgctcatcaa gatatagctg gcttagagca
     1621 actgttggag gaagtcgagc aagaacagat ggcagagact tatcgtcgtt tgaatgctat
     1681 tgtaatcaaa gatgccatcc actcactaga taaaagctac ccactagcag aggaggatag
     1741 cgagtttttg accacctatc tctatgctat cgagtcttgg acctggtttg aactctatct
     1801 cttttgcaat actctgccct tcttgagcaa tcaagatctg atctttttat caacctcctt
     1861 actcgaaaaa tccaaagaat ttaaagagtt agtacacaat cgattgtata tgaagcaagg
     1921 actcttaaat atcttatcag aactcatgga gcgaaaactt ttcttataca tcccaatctt
     1981 cgaagccgag ttggagagca tgttgcgtcc gtacgatgtt tttgaaaaag tatcgtggca
     2041 atttttaaag aagatgagtg tctttcttca aacaaaggga agcaatcaaa aagagattga
     2101 acgctttacc caatctctgc aagtattaga aaatccacaa ttaatagccc tttttgaatt
     2161 gcgtttgcag caatacaaaa agcttatcga ttagttagaa atggcaaaat aataataatc
     2221 tcagcaacga tcgtcaataa tgttaaaact tgtttcttca tattacttcc ctccttgtta
//
LOCUS       DAVMHG010000184         2203 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVMHG010000184 DAVMHG010000000
VERSION     DAVMHG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.187"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..151
                     /gene="comC"
                     /locus_tag="VYR08_001996"
     CDS             26..151
                     /gene="comC"
                     /locus_tag="VYR08_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV3467465.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            172..1497
                     /locus_tag="VYR08_001997"
     CDS             172..1497
                     /locus_tag="VYR08_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV3467466.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            1494..>2203
                     /locus_tag="VYR08_001998"
     CDS             1494..>2203
                     /locus_tag="VYR08_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV3467467.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLK"
BASE COUNT          756 a          287 c          410 g          750 t
ORIGIN      
        1 aaaaaacatt ttaggagatt ttattatgaa aaacacagtt aaattggaac agtttgtagc
       61 tttgaaggaa aaagacttac aaaagattaa aggtggggag atgaggttgt caaaattctt
      121 ccgtgatttt attttacaaa gaaaaaagta atgaaataag gggaaagagt aatggattta
      181 tttggatttg ggacggttat tgttcatttt ttaattatta gtcacagtta ccattttatt
      241 tgtaaaggtc aaataaatag aaaagaatta ttcgtttttg gtgcttatac attactaaca
      301 gaaatagtat ttgattttcc cttatatatt ctatatttag atgggttagg gattgaaaga
      361 tttttatttc ctttgggatt atattcctat tttcgatgga tgaaacagta tgagagggat
      421 agaggactat tcctaagttt actactatct cttttatatg agagcactca taactttctg
      481 tccgtaactt tctcctctat aacaggagat aattttgttt tacaatatca tttcccattc
      541 tttttcgttg tgacggtgtt aacctatttt gttacattaa aaatcattta ctatttccat
      601 ttggaactag cctattttga cgaggactac ctttatcctt tcttgaaaaa agtatttttt
      661 gctttactat tgctacatat tgtatctttc gtttcagata tggtaagtac gattaaacat
      721 ttgaatagtt ttggaagtat tttgtcatct attgtcttta tctctctcct tttgaccttc
      781 tttgcaatga attctcataa agttcaaatg gagaaagaga ttgctttgaa gcagaagaaa
      841 tttgaacaga aacatttaca gaattacaca gatgaaattg ttggtctgta taatgaaatc
      901 cgtggttttc gacatgatta tgctggaatg cttgtcagca tgcagatggc aattgacagt
      961 ggtaatttac aggaaattga cagaatttac aatgaagttt tagtcaaagc aaatcataaa
     1021 ttgcgttcag ataagtacac ttactttgat ttgaacaaca tagaagactc agctttacga
     1081 agtttggttg ctcagtcaat tgtctatgct cgaaataatg gtgtagagtt tacactggaa
     1141 gtaaaagata cgattaccaa gcttccaatt gaactattgg atttggttcg tatcatgagc
     1201 gttttattga ataatgctgt cgaaggatcg gctgatagct ataaaaagca gatggaagta
     1261 gcagttatta agatggaaac tgaaacagtt attgtgattc agaattcatg taaaatgacg
     1321 atgactcctt caggagatct atttgcctta ggattctcca ctaagggaag aaatcgcgga
     1381 gtcggattaa ataatgtgaa agaactacta gataagtaca acaatattat tttagaaaca
     1441 gagatggaag gcagtacatt tagacaaatc attagattta agagggaatt tgaatgaaag
     1501 ttttaatttt agaagatgtt attgaacatc aagtgagact agagagaata ttggatgaaa
     1561 tttcgaaaga atcgaatatt ccaatatcat acaagacaac gggaaaagtc cgtgaatttg
     1621 aagaatacat tgaaaatgat gaagtaaatc agctttattt cctagatatc gatattcatg
     1681 gaattgagaa aaagggattt gaagtggctc agctcattcg tcattacaat ccttacgcta
     1741 ttatcgtctt tatcactagt cgatcagagt ttgcgactct aacctataaa tatcaggtat
     1801 cagccctaga ttttgttgat aaggatatca atgatgagat gtttaagaag agaattgagc
     1861 aaaatatctt ctacacgaag agtatgttac ttgaaaatga agatgttgta gattatttcg
     1921 actacaatta caagggaaat gatttaaaaa ttccttacca tgatattttg tatattgaaa
     1981 caacaggggt atctcataaa ttgcgcatta ttggtaagaa ttttgcaaaa gagttttatg
     2041 gtaccatgac agatattcag gaaaaggaca aacatactca gcgattttat tctcctcaca
     2101 agtcattttt ggtaaatata ggcaatatca gagaaattga tcgaaaaaac ttagaaattg
     2161 ttttctatga agaccatcgt tgtcctattt caagattaaa aat
//
LOCUS       DAVMHG010000185         2157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVMHG010000185 DAVMHG010000000
VERSION     DAVMHG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.188"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1347
                     /locus_tag="VYR08_001999"
     CDS             <1..1347
                     /locus_tag="VYR08_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV3467468.1"
                     /translation="VFYQSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITE
                     KENIIASLQDSKKYLIDLQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILY
                     FIKVMDEHFGHSIYIYESETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDP
                     TPVLAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAI
                     WGGIYGSFFGYELPFHLISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAE
                     AYNSGFAWCVILLGLLFIAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSG
                     IGAGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVL
                     FILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            1363..1839
                     /locus_tag="VYR08_002000"
     CDS             1363..1839
                     /locus_tag="VYR08_002000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV3467469.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1873..>2157
                     /locus_tag="VYR08_002001"
     CDS             1873..>2157
                     /locus_tag="VYR08_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467470.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEME"
BASE COUNT          692 a          288 c          422 g          755 t
ORIGIN      
        1 gttttctatc agtctagtta ctctatagat tttgatgaat acttatttga accatttgat
       61 tattctagaa aggaattacc gaagcagcga gtagtagatt tagatcaaga aaacatgcag
      121 ttaataactg aaaaagagaa tattatcgca tcgttgcaag attcaaagaa atatttgata
      181 gatttacaat ggcaaataga ctatatttta tctatctatg ctcgtcaaat ctctaagaat
      241 aactttttgt gcactccgca tctagttgca ttagaaggat ggatagaaga aactcgtatt
      301 ttatatttta taaaagttat ggatgagcat tttggacatt ctatttatat ttatgaatcg
      361 gaaacattga cggataatca agatgaaata cctatcaaat taacgaatca ttctttaatt
      421 gaaccatttg aattattgac agaaatgtat gctctgccca aatattatga gaaagatcct
      481 acacctgtat tagcaccatt ttactttaca ttttttggaa tgatggttgc tgatttaggc
      541 tatggtttac tattgttttt aggaacaatg ttagcattaa aaatttttca tctaccttca
      601 gcaactaaga gatttttaaa attctttaat atattagggg tagccgttgc aatttggggt
      661 ggaatctatg gctcattttt tggatatgag ttgccatttc atctgatatc tacaacctct
      721 gatgtcatga ctatattagt agtgtcagtt gtgtttgggt ttattacagt atttgcaggt
      781 ttgttagctt caggactaca aaaagtaaga atgaagaaat atgcagaagc atataattca
      841 ggatttgcgt ggtgtgttat tctgcttggc ttgttattta ttgctgttgg aatgttgatg
      901 cctgatatga gaccgttatt tgtattaggg aaatgggtat ctatttttaa tgctgtgggg
      961 attttgattg tttctattat tcaaaccaaa agcttgtcag gtattggagc aggattgttt
     1021 aatctatata acatttcatc ttatataggt gatttagtta gtttcactcg attgatggca
     1081 ttaggattat ctggagcaag tatagcatca gctttcaatt taattgttgg tttgtttccg
     1141 ggaatattgg ctaaactgac aattggatta gtattattca ttcttttaca tgcgatcaat
     1201 atttttctat cgttactatc aggatatgtt catggagcac gtctgatatt tgttgaattt
     1261 tttggtaagt tttatgaggg tggaggaaaa ccatttcaac ctttgaaggc ttctgagaaa
     1321 tatattaagg ttattacaaa gaattaatgg aggatatata taatggaaca tttagcaact
     1381 tatttttcaa cctatggagg agctttcttc gctgcattgg gaattgtatt ggcggttgga
     1441 ttaagcggta tggggtctgc ttatggagtt ggtaaggctg ggcaatctgc cgcagcttta
     1501 ctgaaagaac agcctgaaaa gtttgcctca gctttgatat tgcaattatt gcccggaaca
     1561 caaggattat atggttttgt tattggaatt ttaatttggt tgcaattaac tccagaactt
     1621 cctttagaaa aaggcgttgc ttatttcttt gtagctcttc caattgctat tgtaggatac
     1681 ttttcagcta agcatcaagg aaatgtagca gtagcgggaa tgcaaatctt ggctaaaaga
     1741 ccaaaagaat tcatgaaggg agcaatttta gctgccatgg tagaaaccta tgcaattctt
     1801 gcttttgtcg tatcattcat tttgaccctt cgtgtataag aaataaattt gcaattcaaa
     1861 ggaggtgtct aaatgagcaa tttagaaaac ttacgagagt ctgttattga acaagctcat
     1921 gaaaaagggc gtatgaaatt attggattcc aaaaagaaga ttgatgatga atttgaaatg
     1981 caaaagtcgc tcattataaa gaaaaaagaa gctgaacatg aacgaaagtt aaaagaattg
     2041 caacagaaat atcaaataat ttttcaacaa ttaaaaaata aggaacgcca atcaacgtta
     2101 gtatcaaaac agaaaatatt aaaagaactt tttcaatctg ctttactaga aatggaa
//
LOCUS       DAVMHG010000186         2105 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVMHG010000186 DAVMHG010000000
VERSION     DAVMHG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.189"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            148..804
                     /locus_tag="VYR08_002002"
     CDS             148..804
                     /locus_tag="VYR08_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV3467471.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            794..1117
                     /locus_tag="VYR08_002003"
     CDS             794..1117
                     /locus_tag="VYR08_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV3467472.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1221..2051
                     /locus_tag="VYR08_002004"
     CDS             1221..2051
                     /locus_tag="VYR08_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV3467473.1"
                     /translation="MKKIVKYSSLVALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
BASE COUNT          551 a          408 c          484 g          662 t
ORIGIN      
        1 tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
       61 tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat gaaagaaaaa
      121 ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt cagtatcggc
      181 ctggcctgtg ggactatcgg tgcgccctat gtgacacctg ttgagatggg cttgatgagt
      241 ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt ggttcaagct
      301 cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt tttgttgagt
      361 ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg tatgtctagt
      421 atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac agacaaggta
      481 aatcctatgt ggatgcacgg aaacaatctt aacagctatg tggcttggtt tgtggggaca
      541 gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg cttggatttt
      601 gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat gcaaagacgg
      661 attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt ctttttgctc
      721 ttgacagtgg tgtctcagtc actagctgtt ctgtttgcga cgctacttgg ttgtagcatg
      781 ggggtggttt tagatggtca gtaagtatct tttattagca gttattttct ctggcttggt
      841 gacttggatt ctccgtatga ttcccttcat cttagtcaag tataagggct tgcctgcaat
      901 cgttgagcgt tttttgaagt tcttgcccgt ttccattatc tttgccttga ttctttcaag
      961 cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa tggctagact tcttagccgt
     1021 ctttccaaca gcttgggtag cctttcgcta ccgcaatcta gtcggaacag ttctctttgg
     1081 agtggtcttg attgccatct tgcgtttggt cttttaaatt acccataaaa aaaacttatc
     1141 acagagatag atatcatata atggcgtaaa tgctcttttt ctgttaagat tataaggtat
     1201 tctattttgg aggaaatgac atgaaaaaaa tcgttaaata ctcatctctt gttgccctag
     1261 gacttgttgc tgcaggtgtg cttgcggctt gctcaggggg tgctaagaaa gaaggagaag
     1321 cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc accaaggcca tttatctatg
     1381 aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt tcgcgctatc tttaaagatt
     1441 ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc aggtgtcttt gctggtcttg
     1501 acgctgaccg ttacaatatg gctgtcaaca atcttagcta cactaaagaa cgtgcggaga
     1561 aatacctcta tgccgcacca attgcccaaa atcctaatgt ccttgtcgtg aagaaagatg
     1621 actctagtat caagtctctc gatgatatcg gtggaaaatc gacggaagtc gttcaagcca
     1681 ctacatcagc taagcagtta gaagcataca atgctgaaca cacggacaac ccaactatcc
     1741 ttaactatac taaggcagac ttccaacaaa tcatggtacg tttgagcgat ggacaatttg
     1801 actataagat ttttgataaa atcggtgttg aaacagtgat caagaaccaa ggtttggaca
     1861 acttgaaagt tatcgaactt ccaagcgacc aacaaccgta cgtttaccca cttcttgctc
     1921 agggtcaaga tgagttgaaa tcgtttgtag acaaacgcat caaagaactt tataaagatg
     1981 gaactcttga aaaattgtct aaacaattct tcggagacac ttatctaccg gcagaagctg
     2041 atattaaata atttcttgaa atcatccatt ctgagtaagg tgggtggttt ctcagttttt
     2101 aggga
//
LOCUS       DAVMHG010000187         2094 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVMHG010000187 DAVMHG010000000
VERSION     DAVMHG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.190"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            47..1591
                     /locus_tag="VYR08_002005"
     CDS             47..1591
                     /locus_tag="VYR08_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467474.1"
                     /translation="MLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGIIFFDWV
                     VKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQ
                     LFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQN
                     EAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFL
                     NIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFL
                     KLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLI
                     LGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGT
                     IRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLAR
                     GLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQ
                     FPKDFI"
     gene            1611..>2094
                     /locus_tag="VYR08_002006"
     CDS             1611..>2094
                     /locus_tag="VYR08_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467475.1"
                     /translation="MRFYCEAYEVITEEKKVYFNDIELEVKYSSVEELFIICEKLLDK
                     KKVSFFYVDEKPLRYLLFDYIFLLVLAKKNIPILDGVSNKQVDPTLLHHFSLEIEKNF
                     IDFCYKNMDLILKTQSISLCHREELIIVDVSDDSKFGVFYKRFRTLVDKSNGKYVCVY
                     N"
BASE COUNT          732 a          242 c          385 g          735 t
ORIGIN      
        1 aaaaaaattt agtttattaa cgatatttat tgttgctggt caattattgc taatttatgc
       61 agcaactatt aatgctctgg tgttgaatga attaattgcg atgaatttag agcggttttt
      121 gaaattgtca atctaccaaa tgattgtctg gtgtgggata atattctttg actgggtagt
      181 gaaaaattat caggttgaag tgatccaaga gtttaatcta gagattcgaa atagagttgc
      241 cacagacatc tctaactcta cctatcaaga atttcatagt aaatcatcag gaacatatct
      301 ttcgtggcta aataatgatg ttcagacttt aaatgatcag gcttttaaac aacttttttt
      361 agtaataaaa ggaatttctg gtactatatt tgcagttgtg actcttaatc actatcattg
      421 gtcattgact gtagccacct tgttttcatt aatgattatg ctacttgtac caaaaatctt
      481 tgcatcgaaa atgcgagaag ttagtctaaa tttaactaac caaaatgaag cttttttaaa
      541 atctagtgag actatattga atggatttga tgtgttagcg tccttgaatc ttttatatgt
      601 attgcctaag aaaattaaag aagcaggaat tttattaaag atggttatac aaagaaagac
      661 aactgtagaa acgttagcag gcgctattag cttctttctc aatatttttt ttcagatatc
      721 tctcgttttt ttaacaggct atcttgcaat aaaaggaata gtgaaaattg gtactattga
      781 agcaatagga gcactaacag gtgttatttt tacagcgcta ggtgaattag gaggtcaatt
      841 atcctctatt attggtacga agcctatttt tttaaaattg tattcaatta atccaattga
      901 gtcaaataaa atgaatgata tcgaaccaaa tgaggtgaat agagattttc cgttatatga
      961 agcaaaaaat atttgctata agtatggaga taaagaaata ttaaaaaact taaatttttg
     1021 ttttcaacgt aatgaaaagt atttaatttt aggtgaaagt ggaagcggaa aatctacatt
     1081 attaaaatta ttgaatggct ttttgagaga ttatagtgga gaattgcgat tctgcgggga
     1141 tgatataaaa aaaacctcct atttaaatat ggtttcgaat gttctatatg tagatcaaaa
     1201 agcttatttg tttgaaggta cgattagaga taatatttta ttggaagaaa attatactga
     1261 tgaagaaata ctacagtctt tagagcaagt tggtttgagt gtaaaagatt ttcctaataa
     1321 cattttagat tattatgttg gtgatgatgg gagattactg tcaggagggc agaaacaaaa
     1381 aattacttta gctagagggc taattagaaa taagaaaata gtattaattg acgagggaac
     1441 ttctgctatc gataggagaa cttcgttagc gattgaacgt aagatattag atagagagga
     1501 tttgactgtc attattgtta cccatgctcc gcatccggaa cttaaacaat attttactaa
     1561 gatatatcaa tttccaaagg attttattta agaatatagg ggagggaaac atgagattct
     1621 attgtgaagc ttatgaggta attactgaag agaaaaaagt atactttaat gatattgagt
     1681 tagaagttaa gtattcctct gtcgaagaat tgtttataat atgtgagaaa ttattagata
     1741 agaaaaaagt atcatttttt tatgtagatg agaaaccctt aaggtatctg ttatttgact
     1801 atatatttct actagttcta gctaaaaaaa atatcccaat cttagatgga gtttccaata
     1861 agcaagttga tccaacgttg ctgcatcact tttctttgga aattgaaaag aattttatcg
     1921 atttttgcta taaaaatatg gatctcatcc taaaaactca aagtatatct ttgtgtcata
     1981 gagaagagct tattattgta gatgtttcag atgactctaa atttggtgtt ttttacaagc
     2041 gttttagaac actggttgat aaaagtaacg gaaaatatgt ttgtgtttat aatt
//
LOCUS       DAVMHG010000188         2067 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVMHG010000188 DAVMHG010000000
VERSION     DAVMHG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.191"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1668
                     /locus_tag="VYR08_002007"
     CDS             <1..1668
                     /locus_tag="VYR08_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV3467476.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYG
                     VNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNGGFAP
                     YGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPL
                     FDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDE
                     VWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKY
                     KDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMK
                     VDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDK
                     IDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEI
                     QVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
BASE COUNT          774 a          260 c          465 g          568 t
ORIGIN      
        1 aaaataaaag tatacctcta tacacgagta tctacgtcaa tacagataga ggggtattcg
       61 ttagaggcac aaaaatcacg aatgaaagct tttgctattt acaatgatta tgaaattgtt
      121 ggagaatatg aagatgccgg gaagtctggc aagtctattg aaggtagaat acagttcaat
      181 cgaatgatgg aagatataaa atctggaaaa gatggagtat cttttgttct tgtgtttaag
      241 ttatcaagat ttgcaagaaa tgccgctgat gttctttcta cacttcagat aatgcaggat
      301 tatggagtta atcttatctg cgttgaagat ggcattgatt catctaaaga tgctgggaaa
      361 ctaatgattt ctgttttatc agctgtggct gaaatagaaa gagaaaacat ccgtattcaa
      421 acaatggaag gtcgcattca aaaagcaagg gaaggaaaat ggaatggtgg ctttgctccg
      481 tatggatata aacttgaaga cggcaagctg tttataaatg aggaagaggc agttgcaata
      541 agaacgattt tcgaccagta tgtaaatact acgataggag ccaatgggat atctaaatac
      601 ttagagaatc atggaattag aaaaatccca agacagaatg gtaagaatcc tttgtttgat
      661 gcaggtctta taagaaagat attaaagaat cctgtatata atgggaaaat agcatttgga
      721 agaagaactt tagaaaaagt tcatggtaca agaaatgaat ataagcaggt tgaacaagat
      781 gaatatctaa tatctgaagg gatacatgaa gctatagttt ccgatgaagt ttggcaagct
      841 gctcaggtta agctaaaatc tcaagcaaag aaatatgagc atgtgaataa aggaaaagat
      901 acacgcacac acttgctttc tggaattgta aaatgcccga tatgtggagt gggaatgttt
      961 gggaacaagt gtatcaagaa aaagaaagat ggcacaaagt ataaagattt ttattactat
     1021 ggttataaac ataggcagat gataagaggt cataagtgta cattcagtaa gcaaattaga
     1081 gaagaattgt tagatgatgc tgttgcagag gtgattgtca agatagtaag taatccgaaa
     1141 tttgcttcta tgatgcaaga aaaaatcaac atgaaggtgg atacctctga aatagaaaaa
     1201 gaaatagata attaccaaaa ggaattgaga aagagtcatt ctacaaagtt taagctaatt
     1261 gaggaaatag ataatttaga tgttgaagat aagcattata agagaaggaa acaggattta
     1321 gatgatagac tatatcgtat gtatgacaaa atagatgaat tagaatcatc actaattgat
     1381 gcgaaagcaa agaaacagac tattgaagct gaaaagctta caggagataa catatataag
     1441 gttctgatct attttgataa gctctataaa gtcatgaatg atgtagagcg taggcagtta
     1501 atttcagctt tgatttctga aattcaagtt tatgaagaaa aacaatctaa cggacaatgg
     1561 ctaaaatcca ttacttttaa gctaccaatt atagaagaaa atctcaacat agatttggac
     1621 aatgatgaac aagttgaatg tgtaagttta ttagagaaac gtagttaatc ctcaaaaggt
     1681 tccccaaact ttttgagtcc cactgacgca tcacgctgag gcggtatcac tgcttgtacg
     1741 agttgaagca tcagcgaagt aaaagtagat gttcagccaa tgcgacgagg tgagaccgaa
     1801 ggtcgataag gtgtgttctg ggtataggac tcgtaaagta agtaggaaat atgatgagat
     1861 gatacagaga gcctgaattg gtcaggtgac gtgcttattt acattagcct gataatgaaa
     1921 atggagagat tatcgatggt tatattggaa ttgtaccaaa atgattattc taaagattta
     1981 gtagcatttg attccataga agatgggaaa gcctttgtgg ctcaaatccc gggatacact
     2041 ctggaaacag aagatggttt tgaagtg
//
LOCUS       DAVMHG010000189         2051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVMHG010000189 DAVMHG010000000
VERSION     DAVMHG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.192"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            95..532
                     /locus_tag="VYR08_002008"
     CDS             95..532
                     /locus_tag="VYR08_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467477.1"
                     /translation="MDIKRLEIYHTPTEKSVICLCLNTDQFTVDCEQYIIDRYRHFTV
                     NPKVRFYSRIDKLIDEIIREVKKPPFTFRMRLEKRHSITSKNILYEFQLFDRNSFKTD
                     GLSEVIEQEYLRPEVDLVVIYTGIQGESKADTDDKGRFYGLKP"
     gene            561..674
                     /locus_tag="VYR08_002009"
     CDS             561..674
                     /locus_tag="VYR08_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3467478.1"
                     /translation="MPINPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQRM"
     gene            709..1914
                     /locus_tag="VYR08_002010"
     CDS             709..1914
                     /locus_tag="VYR08_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV3467479.1"
                     /translation="MEGFLLNEQTWLQQLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLFEALERFNPDAPLEMLFDYVRIRFPTTDVQHVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDALVAGGVMKRLDLAINDKTGILNIPVLTEKCRQEECISVFRSFKSYRSGELVRKDE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEKTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHEQILKQQSAK
                     DKDVITTKK"
BASE COUNT          682 a          377 c          432 g          560 t
ORIGIN      
        1 atgacttttt agaggaaatt aaaaagttat ccaacagcag acaggacaca aaaaagccat
       61 catcagaaca acaagaaatg gagaaataaa agtaatggat attaaaagat tggagatata
      121 tcatacaccg actgaaaaat ctgtcatttg tctttgcttg aatacagacc aattcacggt
      181 agattgtgaa caatacatca ttgaccgata caggcatttt accgtcaatc caaaagtacg
      241 attctattcc agaatagata agctgattga tgaaattatc cgtgaagtga agaaaccacc
      301 gttcacattc agaatgaggt tagagaagcg acatagtatt accagcaaaa atattctata
      361 tgaatttcag ctttttgata gaaattcatt caaaacggac ggtctatcag aagtgattga
      421 acaggaatat ttaagaccag aagttgattt ggtagtgatt tacactggca tacagggaga
      481 atcaaaggca gatacagacg acaaaggcag gttttacggt cttaaaccgt aagttctggc
      541 tttgtcgggc agggcttgcc ctgcccatta accccccgta tctaacaggg gggtacaaat
      601 cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg ttacaaggga tttacaaaat
      661 ttcagcgtat gtaaaatggg ctttaaaagt tgacatacgc ctttttgatt ggagggattt
      721 ttactgaatg aacaaacttg gttacagcag ttaaaagaaa aacgcttggc ttatggacta
      781 tctcaaaatc gtttagcggt tgcgactggt attacaaggc agtatctaag cgatattgaa
      841 acaggaaaag tcaagccatc agaggattta cagcaatctc tttttgaagc tctggaacgc
      901 ttcaatcccg acgctcccct tgaaatgctc tttgattatg taaggattcg ctttcccaca
      961 acggacgtac agcatgtggt cgaaaacatc ttacaactga aactgtccta ttttcttcat
     1021 gaggactatg gtttctattc ttattcagag cattatgctt taggcgatat attcgttctc
     1081 tgctcccacg aactggacaa aggagttctg gtggaattga aaggtcgtgg gtgtcggcaa
     1141 tttgaaagct atcttctggc tcaacaaaga agctggtatg agttctttat ggacgctttg
     1201 gtggctggcg gtgtgatgaa acgccttgac cttgccatta acgataagac agggattttg
     1261 aatatccctg tactcactga aaaatgcaga caggaagaat gtatatccgt cttccgcagt
     1321 ttcaaaagct atcgcagtgg cgaactggta cgcaaagatg aaaaggaatg tatgggaaat
     1381 accctctata tcggttcatt acagagtgaa gtttatttct gtatctatga aaaggactat
     1441 gaacagtaca agaaaaatga tattcccatt gaagacgcag aagtaaaaaa ccgttttgag
     1501 attcggctga aaaatgagcg tgcctattat gcagtccgtg atttactcgt ctatgacaat
     1561 ccagaaaaga ctgcttttaa aattatcaat cggtatatcc gttttgtaga taaagacgat
     1621 tccaaacctc gttctgattg gaagctgaat gaagaatggg cttggtttat tgggaacaac
     1681 cgtgaacgat taaaactaac cacaaaacca gagccttact ctttccagcg gacgctgaac
     1741 tggctttccc atcaagtcgc acccacttta aaggttgcga ttaaacttga tgaaatcaac
     1801 cagacgcagg ttgtgaaaga cattctcgac catgcgaaac tgacagaccg gcatgagcag
     1861 attttgaagc aacagtcggc aaaagataag gacgtgataa cgacaaagaa ataacctaaa
     1921 cacaaattca ttgaatatag agaggagaac atttttatga attttggaca aaacctttat
     1981 aactggtttc tatcaaacgc tcaatcactg gtacttttag caatcgttgt gattggcttg
     2041 tatcttggct t
//
LOCUS       DAVMHG010000190         2026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVMHG010000190 DAVMHG010000000
VERSION     DAVMHG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.193"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            196..>2026
                     /locus_tag="VYR08_002011"
     CDS             196..>2026
                     /locus_tag="VYR08_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV3467480.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSEKLASECKALKILNEWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQ
                     LAKKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVE
                     LFTTHFIEIFENSQNKNLYSGQAGILLVGLYFLSKGEIDNKLGE"
BASE COUNT          721 a          255 c          407 g          643 t
ORIGIN      
        1 ccaaaggatt ctcccttttt atttaaataa gtctgtctag ctatagatat attaagtttg
       61 ataaaatttc ttggagattt tgttatggat tataatttca atttggagca tcctttcttt
      121 ttcactaata atgattatag tacagatact agcataaagt accaagtaag tctgcctttt
      181 aattggcacg aagtaatgaa taatgatgag tgggtttatc aataccctat tggaaaattt
      241 gttgaaagac aagggtggaa aatacacatt agttcagagt ataacagttc acatgaattg
      301 ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc catttaaaca tcttagtaca
      361 gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc gaggattttc tggtaaattt
      421 attacatgtt atccaaatca aaatgaatta gagtcggttt tgcaaaggtt agaaagtgcc
      481 ttaaagcaat ataatgggcc atatatttta agcgataaac gttgggatga agcaccaata
      541 tatctgaggt atggtgtgtt taggccgtca agggatgatg aaaagaaggt tgctattgat
      601 gaattaattg ttggcgatga agttgttaaa gatgaacgtt taccagtatt taaaattcca
      661 aaaggtatag tgccccctga ctttttgaat aaatggcttg acaagaaaga taaaaaacag
      721 ggagactttc cctttattat agataatgca attagatttt caaacagtgg tgggatatat
      781 aatgctaggc taaaagaaga tgggaaaaaa ataattttaa aagaggctag accgtataca
      841 ggtctaggat ttgatggtac ttattcttca gagaaattag cttcggagtg caaagcacta
      901 aaaattctaa atgaatggag tgagacgcct aaaatatact ggcacggtaa gatctgggag
      961 cacaccttct taggaattga acatatgaag ggcgttccac taaatagatg ggttaccaat
     1021 aattttcctt tatatgaagt tgttgataag accaaagatt atctattgag agtttcaaag
     1081 atagtagaaa aactcatcga tttaacgaat aagtttcatt ctgaaaatgt atatcatcaa
     1141 gatttacatt taggaaatat cttggttaag gatgaagatg agattagtat aatagactgg
     1201 gaacaggctg tcttttcaaa tgatgaaaaa gttgtacata aagttgcagc ccctggtttt
     1261 agagcatggc gagaaacact tcccagtgag atagattggt atggtattag acaaatagct
     1321 cattatttat acatgccact agtaactaca tcggatttga catacaacta tgtaagtcaa
     1381 accaggatag aaggaaaaaa actatttgag tctttgggtt atacaagaga acatatagat
     1441 tatgtagaaa gtttattgtc atatctagat tctaaatgtc cacagattga gaatatttcc
     1501 aggaaaaaag ttttaaaacc catgcatgag attagaacta tcgagagtga gcaagatata
     1561 caagatttta ttataaaatt attgagaggg tttacgctaa cttatggaca gtggcgtaaa
     1621 gaatttcaat caagattctt cccagttcat tattatggtc tgaattttaa tcaaggaatt
     1681 gcgttttcag atttagctat cctttggagt tatcaacaac tagctaagaa agtaaaaaat
     1741 ttcaagtttg atgactatta tgagattcga acacaggtta ttaatgaggc agtaaataat
     1801 tttaagaaat cgtccctttc tggtcttttt gatggtaaaa ttggtacaat ctggctcatt
     1861 tatgaatttg gggagataga tcgggcagtt gaacttttta caactcattt tatagagata
     1921 tttgagaatt cacaaaataa aaatttatat agtgggcagg ctggaatttt actggttgga
     1981 ctgtattttc ttagtaaagg ggaaattgat aataaactag gagaag
//
LOCUS       DAVMHG010000191         2008 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVMHG010000191 DAVMHG010000000
VERSION     DAVMHG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.194"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            129..1310
                     /locus_tag="VYR08_002012"
     CDS             129..1310
                     /locus_tag="VYR08_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3467481.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1368..>2008
                     /locus_tag="VYR08_002013"
     CDS             1368..>2008
                     /locus_tag="VYR08_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV3467482.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQ"
BASE COUNT          727 a          352 c          369 g          560 t
ORIGIN      
        1 atagatttca attaagaaaa tcacagtttc aaaagagtta tccacagttt gtgtaaaact
       61 tttgtgttta agttataatt aagctagtca gtttatactt tcagtaattc aaacatatgg
      121 aggcaaatat gaaacatcta aaaacatttt acaaaaaatg gtttcaatta ttagtcgtta
      181 tcgtcattag cttttttagt ggagccttgg gtagtttttc aataactcaa ctaactcaaa
      241 aaagtagtgt aaacaactct aacaacaata gtactattac acaaactgcc tataagaacg
      301 aaaattcaac aacacaggct gttaacaaag taaaagatgc tgttgtttct gttattactt
      361 attcggcaaa cagacaaaat agcgtatttg gcaatgatga tactgacaca gattctcagc
      421 gaatctctag tgaaggatct ggagttattt ataaaaagaa tgataaagaa gcttacatcg
      481 tcaccaacaa tcacgttata aatggcgcca gcaaagtaga tattagattg tcagatggga
      541 ctaaagtacc tggagaaatt gtcggagctg acactttctc tgatattgct gtcgtcaaaa
      601 tctcttcaga aaaagtgaca acagtagctg agtttggtga ttctagtaag ttaactgtag
      661 gagaaactgc tattgccatc ggtagcccgt taggttctga atatgcaaat actgtcactc
      721 aaggtatcgt atccagtctc aatagaaatg tatccttaag atcggaagat ggacaagcta
      781 tttctacaaa agccatccaa actgatactg ctattaaccc aggtaactct ggcggcccac
      841 tgatcaatat tcaagggcag gttatcggaa ttacctcaag taaaattgct acaaatggag
      901 gaacatctgt agaaggtctt ggtttcgcaa ttcctgcaaa tgatgctatc aatattattg
      961 aacagttaga aaaaaacgga aaagtgacgc gtccagcttt gggaatccag atggttaatt
     1021 tatctaatgt gagtacaagc gacatcagaa gactcaatat tccaagtaat gttacatctg
     1081 gtgtagttgt tcgttcggta caaagtaata tgcctgccaa tggtcacctt gaaaaatacg
     1141 atgtaattac aaaagtagat gacaaagaga ttgcttcatc aacagactta caaagtgctc
     1201 tttacaacca ttctatcgga gacaccatta agataaccta ctatcgtaac gggaaagaag
     1261 aaactacctc tatcaaactt aacaagagtt caggtgattt agaatcttga ttgacatcta
     1321 tgtaaagaaa gctttacata agagaaaaga tgtgttagtg tagaatcatg gaaaaatttg
     1381 aaatgatttc tatcacagat atacaaaaaa atccctatca accccgaaaa gaatttgata
     1441 gagaaaaact agatgaacta gcacagtcta tcaaagaaaa tggggtcatt caaccgatta
     1501 ttgttcgtca atctcctgtt attggttatg aaatccttgc aggagagaga cgctatcggg
     1561 cttcactttt agctggtcta cggtctatcc cagctgttgt taaacagatt tcagaccaag
     1621 agatgatggt ccagtccatt attgaaaatt tacagagaga aaatttaaac ccaatagaag
     1681 aagcacgcgc ctatgaatct ctcgtagaga aaggattcac ccatgctgaa attgcagata
     1741 agatgggcaa gtctcgtcca tatatcagca actccattcg tttactttcc ttgccagaac
     1801 agattctttc agaagtagaa aatggcaaac tatcacaagc ccatgcgcgt tccctagttg
     1861 ggttaaataa ggaacaacaa gactatttct ttcaacggat tatagaagaa gatatttctg
     1921 taaggaaatt agaagctctt ctgacagaga aaaaacaaaa gaaacagcaa aaaactaatc
     1981 atttcataca aaatgaagaa aaacagtt
//
LOCUS       DAVMHG010000192         1988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVMHG010000192 DAVMHG010000000
VERSION     DAVMHG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.195"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(164..460)
                     /locus_tag="VYR08_002014"
     CDS             complement(164..460)
                     /locus_tag="VYR08_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV3467483.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(990..1883)
                     /locus_tag="VYR08_002015"
     CDS             complement(990..1883)
                     /locus_tag="VYR08_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467484.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          685 a          390 c          223 g          690 t
ORIGIN      
        1 ctactaccaa aaaaagtaaa aataatttcc cttgaaaagg aagatagctt ttaaaataga
       61 ttgacaaact tatgataaca aaaaacaaaa gggttatccc aataaaatta agataaccta
      121 aaatactttc acattctttt ttagaaagta ttttcatcta gttctaccat agagctgctt
      181 tagcagcacc tttctttttc aatatataaa gaactgttgc tgcaataccc gaaccaatag
      241 aacctactcc tgtgattgca cctacaattg aaattactgc tgtaactgta ctataagcac
      301 taatcaaatt gactacagta gcagcagctt ttgtcgaaat tcctaatgtc ccagccaaca
      361 taggaaaaga aacaacacct aagcttaacc aactaatgac tgctaacatt gctgaaacaa
      421 taagaaattt attatgaaat tccattcttt tagatttcat tttagtgtac tccttttgta
      481 tattatgtat gttttattga gagttaattc gcacatgttg tattcctaac tctcttcaaa
      541 tgttcgtcta tattttatac tacttttatt aacatatcta taataaataa attactacta
      601 cattttccta gattatcaaa ttacttttat atcactaaga ctcaataaaa gataatgtct
      661 atttaacttt tatacgatta ctacctcttt ttagtttaca cttcctacat cataaataaa
      721 catactcaac cctcacaatc tccaaataac tcttctatat cattgaaaat tgagtttcat
      781 cctctatact aatgtaggac attcctttaa tccaatataa gaataccttg ttccattaat
      841 ctctacccga tattatagat agaaccaaat tcctatactt aaaaggacaa ataacgttcc
      901 aaccataata aacctccttt ctttttgaga acgttttctt tataggttta gtatagcaaa
      961 aacatgaaaa aaacaacatg acttagaagt caggttgttg ggaaatctat ctgtttttct
     1021 gctaaaattc tctgaactaa tacctgatcg tctagcaatt ctgctagaga aatcgccttt
     1081 tcatagaaac tttctgcctc taaaatattc ttgtctacaa ataaagcata ttttgccttt
     1141 agcaaaaata gatgaggtcg gtaaaaaaca tgtcttgttt tagcaataat tttctcaaat
     1201 gtttgaatat atatcagact atcttctgtc tgaatttgga taaataggct cagaagaact
     1261 ttttccattt gaactaggct agccttatct tcatattctg aacatagcaa tactttttga
     1321 gctaactcct caaaatgttc tttatcttct aatcccatag cacaacaaaa gaaatacaat
     1381 tcaataatca acaaatcatt tacagaaaaa tatttctttt ttaataactg ctggaaatat
     1441 tcttccacta accctaaacc gtaggtcaca tctgaactac cataaatatc aaaacgagct
     1501 tgtgtcacat ccacaattaa ctgctcttcc tcaggcaaat tctcataata gttttcatat
     1561 atttcctcaa gtatagcttc ttttcgttcg attcttccct tatcagcgta ggtttgagaa
     1621 tgaatcagct catctttgag ttttaggaat ccttttggaa tctctataac cttaacatcc
     1681 acaatattct gtaatgatac tccaagttta tccgcaatca ataataattt aggaagtgtt
     1741 gctaaagctt gcccattttc aatacgtgct agctgacgaa tcgtcaactc tgattcatca
     1801 ccacaaatat ctatctgagt ttttccacaa gatcgcctaa tagttttgat tttataacca
     1861 atttcacgtt tcaactcatc catagtaata ctccttaagt tgattacaaa gatttctttt
     1921 tcttcctaat tcttttgtca atcatatata aaacgataag cgaaaacagc ggtagaacaa
     1981 tataactc
//
LOCUS       DAVMHG010000193         1982 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVMHG010000193 DAVMHG010000000
VERSION     DAVMHG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.196"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(493..1629)
                     /gene="dnaJ"
                     /locus_tag="VYR08_002016"
     CDS             complement(493..1629)
                     /gene="dnaJ"
                     /locus_tag="VYR08_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV3467485.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT          568 a          471 c          397 g          546 t
ORIGIN      
        1 atcaaaaagc aaactagaaa gctaggcaca gactgctcaa aacaccgttt taaggttgtg
       61 gatagaactg acgaagtcag ctcaaacact gttttgaggt tgtggataga actgacgaag
      121 tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt tttgaagagt
      181 atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa cacctgaaac
      241 agcttggttt cagctgtccg gaaactttaa aacttaggtt caaagtttag gtatggaact
      301 tcgaaggagg tcactaccgt ccgtcattac ttaggaaagt cttaaaaaat ctataaagta
      361 aaaagcaaac taggaaacta gccacgggct gctcaaaaca ctgttttgag gttgtagata
      421 cgactgacaa agtcagtcac atatatacgg caaggcaacg ctgacgtggt ttgaagagat
      481 tttcgaagag tattattctc catcaaaggc atctttaata tggtcaaaga agcctttttt
      541 ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta ctttttggcg
      601 gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac caactgcacc
      661 gccacgaagg ctcggtgccc ccttactacg taggcggaac ttcttaccag tctgagttcc
      721 ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg tatcaccaag
      781 agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt cacgttcaaa
      841 cttgtcgcta gcttccacag aaactactac atacaagtca ccataaggtc caccgttaaa
      901 gcctgcttca ccttgaccag cgaggcgaat ttgttgacct gtttccacac cagcagggat
      961 tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg ttgtacatgg
     1021 atatttgatt tcttttcctc gaccgtgaca gacatcacag gttacttggc gacgcatcat
     1081 accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc gtccacaagt
     1141 gactggactt gtccctggct tagcaccaga tccattacat gtacgacagc cagcttcacg
     1201 atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca aattgacacg
     1261 atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac cgcctccgcc
     1321 gaagaaactt gagaaaatat cctcaaaacc accgaagcca cctgccccat tgaaaccgcc
     1381 gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact ggtcataggc
     1441 agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct tgtacttgtc
     1501 ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct tacgataagc
     1561 ctttttgatt tcgtctgccg aagcgttttt ggataccccc agacgatcat aaaattcagt
     1621 attgttcata attcaagata caaagggcgt caaacggaca cagccatcta ggagttttga
     1681 cgacggacgc ttacgtccta gaaaaaacta tctttttggc acagtccata gcccgtgttc
     1741 agttacgaac acccttattc ctttctattg atgttctgaa tcgaaccacc atttaggtcg
     1801 ggttcaattc ctttctttta tatttagtaa gaaattctga aacccgtgtt cagttacgaa
     1861 cactctttgt tcctttctat tgatgagaca aaacccaaac aaatgagatt ccgtcccttt
     1921 ttatcgaaaa acagaggctg ggttgcaacc tctgcaaata agtaaattaa ctttattttg
     1981 at
//
LOCUS       DAVMHG010000194         1947 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVMHG010000194 DAVMHG010000000
VERSION     DAVMHG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.197"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            143..1774
                     /locus_tag="VYR08_002017"
     CDS             143..1774
                     /locus_tag="VYR08_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV3467486.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          574 a          405 c          370 g          598 t
ORIGIN      
        1 aaggttaaaa actcttaaaa ataatacact tatcaattat aattttcttt tacaaacttg
       61 acattaacac ttgttagatt tacttttatc ctttaaaata gaataggaga tattccgcga
      121 ttatttttcg gaggaaaaag aaatgtccat taattggcag gaaattttat ttcacttttt
      181 aggtggtctg gggctattct tatatagcat caagaccatg ggagacggtt tacaacaagc
      241 tgctggagat cgccttcgtt tttacattga caaatatact agtaatcctt tctttggagt
      301 tctggttggt attgggatga ctgctctaat tcagtctagt tctggtgtaa cagttatcac
      361 agtcggcctg gtcagtgccg gtctcttaac cttacgtcag gctatcggga ttgtcatggg
      421 tgctaatatt gggacaactg tcacatcctt tctcatcggt tttaaattag gtaactatgc
      481 cctacctatg ctctttatcg gtgccgtctg tctctttttt acaaaaaatc ggacagtcaa
      541 taatatcgga cgcatcctct ttggtatcgg tggtatcttt tttgccctca atctcatgag
      601 cggcgcaatg gctccactca aggatttaca ggtctttaag gactatatga ttgagctaag
      661 taagaatcct gttttgggtg tctttgtcgg tactggcttg accttgctaa ttcaagcttc
      721 ttcggctacc attgggattt tacaaaacct ctacgccggc aatctaattg acctacaggg
      781 agctttgcca gttctatttg gtgacaatat cgggacaacc attacagcca tcattgcctc
      841 tttaggggct aatattgcag ctaaacgggt agcaggagct catgttgcct tcaacattat
      901 cggaacagtc gtctgcgtta tttttctagt tccttttact gtcctgattc attggtttga
      961 agctacgcta aatctagcac cggaaatgac catcgccttt gctcacggaa cctttaatat
     1021 taccaacacc attgtccaat ttccatttat cggagctctg gcttactttg taaccaagat
     1081 tattcctgga gaggacgagg ttgtcaaata cgaaccctta tatcttgatg aacatttcat
     1141 caaacaggcc ccatctatcg ctctaggaaa tgctaagaaa gagctcttgc acttaggaaa
     1201 ctacgctgct aaagcctttg acctttccta taagtacatc attgacttgg atgaaaaagt
     1261 tgctgaaaaa gggcataaaa ccgaagaagc aattaacacc atcgatgagc aattaacacg
     1321 ttatctcatt gccctttcaa gcgaagctct cagccaaaaa gaaagtgaag tgcttaccaa
     1381 tatccttgat tcctcccgtg atttggaacg gattggagac cacacggagg ctctactcaa
     1441 tctgactgac tatcttcaac ggaaaaatgt tgaattttct gatgccgcct tgaaagaatt
     1501 agaggaagtt taccgccaaa ctagtgactt tatcaaagat gctctggata gtgtggaaaa
     1561 caatgatatt gaaaaagcac gcagtcttgt agaacgtcat gaagcaatca ataagataga
     1621 acgtgttctc agaaaaaccc acatcaaacg cctcaacaaa ggcgaatgtt caacacaagc
     1681 tggagtcaac tttatcgaca tcatctcaca ctacactcgt gtatcagacc acgctatgaa
     1741 ccttgctgaa aaggtttttg cagaacaaat ctaataacca agaagctatc catcataatt
     1801 ggatggcttc ttactttttc ctaagcaaga ctaggctgaa tgaaactgaa agagtattct
     1861 gcagatatat aatatattga aactagaata gtacacctct atttctaaaa cattgttaga
     1921 aatcgatttg actgtcctga acgattt
//
LOCUS       DAVMHG010000195         1920 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVMHG010000195 DAVMHG010000000
VERSION     DAVMHG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.198"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            15..218
                     /locus_tag="VYR08_002018"
     CDS             15..218
                     /locus_tag="VYR08_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV3467487.1"
                     /translation="MNTKMMEQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
     gene            536..739
                     /locus_tag="VYR08_002019"
     CDS             536..739
                     /locus_tag="VYR08_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pncT"
                     /protein_id="HEV3467488.1"
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGCIGWGLLKSIGGMIK
                     PAPYVPPVCIPKSSWNPAPPVPC"
     gene            1742..>1920
                     /locus_tag="VYR08_002020"
     CDS             1742..>1920
                     /locus_tag="VYR08_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV3467489.1"
                     /translation="MNYEGSKQLTDARFKCLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATL"
BASE COUNT          637 a          258 c          354 g          671 t
ORIGIN      
        1 aaggagtatt tgtcatgaat acaaaaatga tggaacaatt ttctgttatg gataatgaaa
       61 tgcttgatag gatagaggga ggaatatttg gagttgacga tgctttgttt tgggcagggt
      121 taggctatgt tgcaggtagc attgttgata cagcaattga tgattttacg aatcaatgcc
      181 ggaaaaatcc tcatcagtgg ttctgtgttc gtgtatagat atagtagtgt tgtattgtat
      241 aagaatttat ataacttaat gacctatgca gtgatagaaa ctattctttt gcaaacgagt
      301 cgcttattct gttataagtt tacgtgctcc tagtcacagt tcttgtgctt gatagaaaga
      361 atgacaccta taaaaatgcg agatattggc tatatcactc acttattcac ctccccgtga
      421 tttgtagtag tgataggttt tctcactatt attataaaca ttataaaata aagagtacaa
      481 cactttttca ttctgtgttg tgccttgagt aaaacgaaag gaatgaatta taacaatgga
      541 aaaaattgat tatattactt tgaatgaagt agaattagag acgattagtg gtggagatga
      601 ctgttttatt ggagatatcg gatgtatcgg ctgggggctt ttgaagagta tcggagggat
      661 gataaaacct gctccatatg ttcctccagt atgcattcca aaaagttcgt ggaatcctgc
      721 acctccagtt ccatgttaaa aagttattgt aaacttatat aaaaaatgaa taatcgactt
      781 gttcgctatt ttgttgaatc ttcgcaacta taatatacag gtgttgttca ggattatttt
      841 agcttattgc aagagtcgaa aactagaaac taatggctgg ttggaacgtt aaaaattcac
      901 tttctaacca gctaatactg cttcaaataa tttctgaaca agtctaataa ataaaacgaa
      961 taagatagat tattaataca gtcttgttcg ttttttctca tatattattt aattaataat
     1021 attataaaat tggaaagcag tagggatggg aagtactacc acagatttat tattaatttt
     1081 atcagtattg tttgaaatcc aaaaaaagta tgttaagcta tatttatttg gttctttagt
     1141 ttgattgttg ttgagtataa ctatttttgt atagatgaaa atcttgttat tgttcaagat
     1201 gttttgatga ccattcatga tttgagtaat ccaaggtcaa aatgagtgct atactagcag
     1261 tgtaaagatt cttgttcaac taagataaat cacacattta ggaggaaatt ttatgaagaa
     1321 aaaaatactt atcattttca tcttgtatct aatcatgtcc atctttcttt atccgcttag
     1381 ggaaagtatt tggtatcagc tattttatac catagcctat gtgattgcag ttatgatcta
     1441 ttttgcttta actaaaaaga aaggagcaaa gaaatgaaaa cttttcttgc taaaaaacgg
     1501 aacatcttcc tttcgtttgg gggttgaagt attataaaaa gtagaagtta atgaagtata
     1561 aaagggtaag tgtttttgca atacttaccc ttttataata gattccctgc gaaacaaaat
     1621 atggtatagt atagtagatt gaaactagaa tagtacgcct ctacttctaa aacattgtta
     1681 gaaatcgatt tgactgtcct gattgattta tcctgttctt atttcatttt actatagttc
     1741 tatgaattat gaaggaagta aacaactaac tgatgcacga tttaaatgtc ttgttggtgt
     1801 tcaacgcacg acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
     1861 cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca
//
LOCUS       DAVMHG010000196         1899 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVMHG010000196 DAVMHG010000000
VERSION     DAVMHG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.199"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1899
                     /locus_tag="VYR08_002021"
     CDS             <1..>1899
                     /locus_tag="VYR08_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV3467490.1"
                     /translation="ENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAYD
                     DGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIK
                     ATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKETK
                     AEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYA
                     LKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQA
                     VLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSN
                     VEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWEL
                     RVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGN
                     VYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNN
                     GFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGE
                     VSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDA
                     GYVINLSK"
BASE COUNT          767 a          225 c          368 g          539 t
ORIGIN      
        1 tgaaaataaa gacttaaaga aactcattaa aaagaaattt agagaagttg atgattttac
       61 aagtgaaact ggtaagagaa tagaggaata cgattataaa tacgatgata agggaaatat
      121 tattgcttat gacgatggta gtgccttaca atatgaaact gaaaaacttg acgaaataaa
      181 atcaaaaatt tatggcgttc taagcccatc taaagatgga cactttgaaa ttcttggaaa
      241 gataagcaat gtttctaaaa atgccaaggt atattatgga aatagctata aatcgataga
      301 aatcaaagcg actaagtatg actcacattc aaaaacgatg acatttgatt tatacgctaa
      361 tattaatgat attgtggatg gattagcttt tgctggagat atgcggtttt ttgttaaaga
      421 tgataatcga ataaaagctg aaactaaaat tagaatgcct gaaaaaaata aggaaactaa
      481 agcagaatat ccctatgtat caagttatgg gaatgtaatc gaattaggag aaggagatct
      541 ttcaaagaac aaaccagaca atttaactaa aatggaatct ggtaaaatct attctgattc
      601 agaaaaacaa caatatctgt taaaggataa catcattcta agaaaaggct atgcactaaa
      661 agtgactacc tataatcctg gaaaaacgga tatgttagaa ggaaatggag tctatagcaa
      721 ggaagatata gcaaaaatac aaaaggccaa tcctaatcta agagtccttt cagaaacaac
      781 aatttatgct gatagtagaa atgttgaaga tggaagaagt acccaagctg tattaatgtc
      841 ggctttggac ggctttaaca ttataaggta tcaagtgttt acatttaaaa tgaacgataa
      901 aggggaagct atcgataaag acggaaatct tgtgacagat tcttctaaac ttgtattatt
      961 tggtaaggat gataaagaat acactggaga ggataagtcc aatgtagaag ctataaaaga
     1021 agatggctcc atgttattta ttgataccaa accagtaaac ctttcaatgg ataagaacta
     1081 ctttaatcca tctaaatcta ataaaattta tgtacgaaat ccagaatttt atttaagagg
     1141 taagatttct gataagggtg gttttaactg ggaattgaga gttaatgaat cggttgtaga
     1201 taattattta atctacggag atttacacat tgataacact agagatttta atattaagct
     1261 gaatgttaaa gacggtgaca tcatggactg gggaatgaaa gactataaag caaacggatt
     1321 tccagataag gtaacagata tggatggaaa tgtttatctt caaactggct atagcgattt
     1381 gaatgctaaa gcagttggag tccactatca gtttttatat gataatgtta aacccgaagt
     1441 aaacattgat cctaagggaa atactagtat cgaatatgct gatggaaaat ctgtagtctt
     1501 taacatcaat gataaaagaa ataatggatt cgatggtgag attcaagaac aacatattta
     1561 tgtaaatgga aaagaatata catcatttga tgatattaaa caaataacag acaagacact
     1621 aaacattaag attgttgtaa aagattttgc aagaaataca accgtaaaag aattcatttt
     1681 aaataaagat acgggagagg taagtgaatt aaaacctcat agggtaactg tgaccattca
     1741 aaatggaaaa gaaatgagtt caacgatagt gtcggaagaa gattttattt tacctgttta
     1801 taagggtgaa ttagaaaaag gataccaatt tgatggttgg gaaatttctg gtttcgaagg
     1861 taaaaaagac gctggctatg ttattaatct atcaaaaga
//
LOCUS       DAVMHG010000197         1897 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVMHG010000197 DAVMHG010000000
VERSION     DAVMHG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.200"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1744)
                     /locus_tag="VYR08_002022"
     CDS             complement(<1..1744)
                     /locus_tag="VYR08_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEV3467491.1"
                     /translation="MFKVLRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASINAAVFSGNMIVNGAPAINASLNIA
                     KSETKVYTGTGKDSFYNIPIYYQLTVTNDGSKLTFTYTVTYVNPTTKILGNLSNMRPG
                     YSIYNTGTSTQTMLTLGSGLGIPSGVKNSITDKNGRQVRYYNTSTMTKRGSGYTWVNG
                     AQMNGWYAKKEYGLTSSWTVPITGTDTSFTFTPYAAKTDRIGINYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SASASTSASVSASTSASASASTSASASASTSASASASTSASASASTSASESASTSASV
                     SASTSASASASTSASASASTSASESASTSASASASTSASESASTSALASASASTSASA
                     SASTSASASASTSAS"
     gene            complement(1742..1855)
                     /locus_tag="VYR08_002023"
                     /pseudo
     CDS             complement(1742..1855)
                     /locus_tag="VYR08_002023"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          487 a          411 c          425 g          574 t
ORIGIN      
        1 tacttgcgct agtgcttgct gaagccgaag cactggtact tgctgaggct gaggcacttg
       61 ttgacgctga cgctgaggct aaggcagacg ttgatgccga ttcagaggca cttgttgacg
      121 ctgaggctga ggcagacgtt gatgctgatt cagacgcact tgttgaggcc gaagctgatg
      181 cacttgttga tgctgaagct gaagcacttg ttgatgctga gactgacgca ctcgttgatg
      241 ccgattcaga cgctgaggtg cttgctgagg ctgacgcact cgttgatgct gaagctgacg
      301 cacttgtcga cgctgaagcc gacgcacttg tgcttgctga agctgaagca cttgtgcttg
      361 ctgagactga tgcactggtt gacgctgaag ccgaggcact ggtacttgct gaagctgaag
      421 cactcgttga tgcggattca gacgcacttg ttgatgctga agccgacgca ctcgttgatg
      481 ctgaagccga cgcacttgtt gatgctgaag ctgaggcgct ttgactagca cttactgaga
      541 gtgacttaga ctgtgaaagt gactgactgg tcgtgctaga ttcaactacc tttcctctac
      601 ccttgaagta gttaattcca attctatctg ttttagcagc gtaaggggta aatgtaaagg
      661 atgtatccgt tccagtaatt ggtacagtcc aagatgatgt taatccatat tctttcttag
      721 cataccaacc attcatttgg gcaccattta cccaagtata cccactaccc cgcttcgtca
      781 ttgtagatgt attatagtat cgaacctgtc taccattttt gtcagtaatg gagttcttta
      841 cacctgaagg gataccaaga ccactgccaa gggttaacat tgtttgtgtt gaagtacctg
      901 tattatagat agaataccca ggacgcatat ttgaaagatt accaagaatc tttgttgtag
      961 gattcacata cgtaaccgta taggtaaagg tcaattttga accatcattt gtcactgtca
     1021 attgatagta aattggaata ttataaaatg aatctttacc tgtacccgtg taaacttttg
     1081 tctcactttt agcaatgttt agacttgcat taattgcagg agcgccattt acaatcatat
     1141 tgccagaaaa aacagcagca tttatagaag ccagcaattg ctcgatggaa tccactgaac
     1201 gcttgcgtcg cttagcataa gattggagat tgacatttgt aactgatgct acataatcac
     1261 tagctggttt cttacgatct tcttcgacct tcttagcagt tgcttctgta gcggcagctg
     1321 tttgtgaacc taccacagta gatgatgcag agatacttgt cgaagccgat gtacttgctg
     1381 attctgatgc acttgtactc gctgaggtcg aagcgctggt tgaggctgac tcagatgcag
     1441 atgtacttgc cgactctgaa gctgacaaac tagttgaact cgctgaatta cttgtagata
     1501 tcgtacctag aactactgta ccatttgttg ccaaagcatc cgttttctct acagtttttt
     1561 caagtactgc tgactcattt gtaaatacct tcgtttgagt tgcaacggtt ccacttatga
     1621 cagttcccgc agcaactatc cccttgagga tatcaagccc agtaattgaa tgacttactt
     1681 tatcttctac cgtttcggtc atgacctgag tagtatcaac accacctcgc aagaccttaa
     1741 acaagccaaa aagagaagtc gaggcccgta gccaatgctt ccctgatttg attaatttga
     1801 accgagttac tcggtctgtt tctctatatt cacccttctg acgtctaaaa aatattttga
     1861 aatttccttt cgagtattat tttcatgtat ggatata
//
LOCUS       DAVMHG010000198         1884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVMHG010000198 DAVMHG010000000
VERSION     DAVMHG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.201"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..103
                     /locus_tag="VYR08_002024"
     CDS             <1..103
                     /locus_tag="VYR08_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEV3467492.1"
                     /translation="NEFADFVALVDKSQFACEIAIKVWRNSLKFSII"
     gene            133..>1884
                     /locus_tag="VYR08_002025"
     CDS             133..>1884
                     /locus_tag="VYR08_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV3467493.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMINLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFN"
BASE COUNT          585 a          319 c          399 g          581 t
ORIGIN      
        1 aaatgagttc gcagactttg tagccctggt cgacaaatca caatttgctt gtgagatagc
       61 tataaaagtg tggagaaata gcttgaaatt tagtataata tagctaaact atttgtttaa
      121 agtgagaaaa aaatgggaaa ttttagcttt cttttaaaaa atgacgaata tgaatctttt
      181 tcaaaacctt gcattgaagc tgaaaatatg attgctacat caactgtggc tactgccttt
      241 atggcgcgtc gtgctttgga gcaggctgtc cattggatat atagtcacga ttcatattta
      301 gaagctccct atcgtgctac tctatcttct ttagtatggg atgatgattt tagggatatc
      361 gtagattctg aactccacaa gcagatagtt ctgttgattc ggtggggaaa ccatgctgct
      421 catggtggtg aaattaagga acgagaagcg attttagctt tacatcattt gtatcagttt
      481 gttaatttta tcgattattg ttacagcaat gaatttgtgg agcgttattt tgatgagaag
      541 tgcttaccac tttcagcaaa catcaaatac cgagaaactc cacaatctat gataaactta
      601 caagacagtt taccagaact gcctgatttt catgaacaga tggctgctca gtccgtagaa
      661 gttcaagaga cttatactga aaaacgtgag actgcagcgc aacggcaaga tgtgcctttc
      721 catattgatc aattatctga ggcagagaca agaaagctct ttattgatat cgatctccgt
      781 ttagcaggat ggatatttga agaaaactgt cgtgttgaga tagccgttga tggtctcaag
      841 cacggttcag gaattggtta ctgtgactat gtactttatg gtaaaaatgg gaaaatttta
      901 gcgattgtgg aggctaaaaa agcctctgtc aatccagaag taggggaagt acaggtcaaa
      961 gaatatgctg aagctcttga gaaacatatc ggctatcagc caatttgctt tattacaaat
     1021 gggttgaagc actatatact tgatggtccg aaccgccgcc agattgcagg cttttactct
     1081 caagaagaat tgcaattagt gatggataga cgtcatcttc aaaaaccgct tgaggatatt
     1141 tctagtaaaa ttagggacga tatttccggg cgtcactacc aaaaacatgc cattgcaagc
     1201 gtttgtgaag ctttctctaa tcatcgtaga caggcacttt tggttatggc aactggggcg
     1261 gggaaaactc gtacagcagt ttctctagtt gatatcttat cacgtcataa ctgggtaaaa
     1321 aacgttctct tcttagccga tagaacttcc ttggttaagc aagcctatga ttcgtttaga
     1381 aaattactcc cagatctttc cgtttgtaac ttcttagaag ataaagaagg agctcaatca
     1441 agtcgtatgg tcttttcaac ttatccgacc atgattggag cgattagtgg tcaagaagaa
     1501 gtaaatcaac gcccttttac tgttgggcat tttgacctta tcataattga cgaatctcac
     1561 cgttctattt atcagaaata caagtccatt tttgattatt ttgatgcaag aattgtaggc
     1621 ttaacagcta ctccgcgtca agatttagat aaaaacacct atggattctt taatttggag
     1681 aatggggttc caacatatgc atatgatttg gaagaggctg ttaaagacgg atatttagta
     1741 gcctatcatt ctatcgaaac caaactgaaa ctacctacgg atggtctaca ttatgatgat
     1801 ttgtccgaag aagaaaagga acattttgat agcaaatttg aagacaatag ctgtgaaaaa
     1861 gatattgatg ggagtgtatt taat
//
LOCUS       DAVMHG010000199         1879 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVMHG010000199 DAVMHG010000000
VERSION     DAVMHG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.202"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1879
                     /gene="ftsH"
                     /locus_tag="VYR08_002026"
     CDS             <1..>1879
                     /gene="ftsH"
                     /locus_tag="VYR08_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV3467494.1"
                     /translation="GFQYFYSGNNSGGSQQINYTELVQEITDGNVKELTYQPNGSVIE
                     VSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTILPADTTVSELQKLATDHKAEVTVK
                     HESSSGIWINLLVSIVPFGILFFFLFSMMGNMGGGNGRNPMSFGRSKAKAANKEDIKV
                     RFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGARIPAGVLLEGPPGTGKTLLAKAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAAPAIIFIDEIDAVGRQRGVGLG
                     GGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNRSDVLDPALLRPGRFDRKVLVGRPD
                     VKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFVGADLENVLNEAALVAARRNKSIID
                     ASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHEAGHTIVGLVLSNARVVHKVTIVPR
                     GRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGRVAEEIIFNVQTTGASNDFEQATQM
                     ARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKSISEQTAYEIDEEVRSLLNEARNKA
                     AEIIQSNRETHKLIAEALLKYETLDSTQIKALYETGKMPEAVEEESHALSYDEVKSKM
                     ND"
BASE COUNT          607 a          318 c          435 g          519 t
ORIGIN      
        1 ggattccagt atttctattc tgggaataac tcaggaggaa gtcagcaaat caactatact
       61 gagttggtac aagaaattac cgatggtaat gtaaaagaat taacttacca accaaatggt
      121 agtgttatcg aagtttctgg tgtctataaa aatcctaaaa caagtaaaga aggaacaggt
      181 attcagtttt tcacgccatc tgttactaag gtagagaaat ttaccagcac tattcttcct
      241 gcagatacta ccgtatcaga attgcaaaaa cttgctactg accataaagc agaagtaact
      301 gttaagcatg aaagttcaag tggtatatgg attaatctac tcgtatccat tgtgccattt
      361 ggaattctat tcttcttcct attctctatg atgggaaata tgggaggagg caatggccgt
      421 aatccaatga gttttggacg tagtaaggct aaagcagcaa ataaagaaga tattaaagta
      481 agattttcag atgttgctgg agctgaggaa gaaaaacaag aactagttga agttgttgag
      541 ttcttaaaag atccaaaacg attcacaaaa cttggagccc gtattccagc aggtgttctt
      601 ttggagggac ctccggggac aggtaagact ttgcttgcta aggcagtcgc tggagaagca
      661 ggtgttccat tctttagtat ctcaggttct gactttgtag aaatgtttgt cggagttgga
      721 gctagtcgtg ttcgctctct ttttgaggat gccaaaaaag cagcaccagc tatcatcttt
      781 atcgatgaaa ttgatgctgt tggacgtcaa cgtggagtcg gtctcggcgg aggtaatgac
      841 gaacgtgaac aaaccttgaa ccaacttttg attgagatgg atggttttga gggaaatgaa
      901 gggattatcg tcatcgctgc gacaaaccgt tcagatgtac ttgatcctgc ccttttgcgt
      961 ccaggacgtt ttgatagaaa agtattggtt ggccgtcctg atgttaaagg tcgtgaagca
     1021 atcttgaaag ttcacgctaa gaacaagcct ttagcagaag atgttgattt gaaattagtg
     1081 gctcaacaaa ctccaggctt tgttggtgct gatttagaga atgtcttgaa tgaagcagct
     1141 ttagttgctg ctcgtcgcaa taaatcgata attgatgctt cagatattga tgaagcagaa
     1201 gatagagtta ttgctggacc ttctaagaaa gataagacag tttcacaaaa agaacgagaa
     1261 ttggttgctt accatgaggc aggacatacc attgttggtc tagtcttgtc gaatgctcgc
     1321 gttgtccata aggttacaat tgtaccacgc ggccgtgcag gcggatacat gattgcactt
     1381 cctaaagagg atcaaatgct tctatctaaa gaagatatga aagagcaatt ggctggctta
     1441 atgggtggac gtgtagctga agaaattatc tttaatgtcc aaactacagg agcttcaaac
     1501 gactttgaac aagcgacaca aatggcacgt gcaatggtta cagagtacgg tatgagtgaa
     1561 aaacttggcc cagtacaata tgaaggaaac catgctatgc ttggtgcaca gagtcctcaa
     1621 aaatcaattt cagaacaaac agcttatgaa attgatgaag aggttcgttc attattaaat
     1681 gaggcacgaa ataaagctgc tgaaattatt cagtcaaatc gtgaaactca caagttaatt
     1741 gcagaagcat tattgaaata cgaaacattg gatagtacac aaattaaagc tctttacgaa
     1801 acaggaaaga tgcctgaagc agtagaagag gaatctcatg cactatccta tgatgaagta
     1861 aagtcaaaaa tgaatgacg
//
LOCUS       DAVMHG010000200         1838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVMHG010000200 DAVMHG010000000
VERSION     DAVMHG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.203"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            92..244
                     /locus_tag="VYR08_002027"
     CDS             92..244
                     /locus_tag="VYR08_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV3467495.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            267..1687
                     /locus_tag="VYR08_002028"
                     /pseudo
     CDS             267..1687
                     /locus_tag="VYR08_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1736..1819
                     /locus_tag="VYR08_002029"
     CDS             1736..1819
                     /locus_tag="VYR08_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEV3467496.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT          617 a          335 c          401 g          485 t
ORIGIN      
        1 atgatttctt ggaggaaatt aaaaagttat ccaacagcag acagtccacg caggcgacgt
       61 gcgaagcgga agtcgcaggt gtggactgat cttgctggct ggtgtggcaa tagccacgcc
      121 agcacttaac cccccgtatc taacaggggg gtacaaatcg acaggaaaca gtcaaaaaaa
      181 cattagaaaa tcctttggtt acaagggatt tacaaaattt cagcgtatgt caaatgggct
      241 ttaaaagttg acatacgcct ttttgattgg agggattttt actgaatgaa caaacttggt
      301 tacagcattt aaaagaaaaa cgcttggctt atggactatc tcaaaaccgt ttagctgttg
      361 cgactggtat tacaaggcag tatctaagcg atattgaaac aggaaaagtc aagccatcag
      421 aggatttaca gcagtccctt tgggaagctc tggaacgctt caatcccgac gctccccttg
      481 aaatgctgtt tgattatgta aggattcgct ttccgacaac agacgtacag caggtggtcg
      541 aaaacatctt acaactgaaa ctgtcctatt ttcttcatga ggactatggt ttctattctt
      601 attcagagca ttatgcttta ggcgacatat tcgtcctttg ctcccatgaa ctggacaaag
      661 gagttctggt ggaattgaaa ggtcgtgggt gcagacaatt tgaaagctat cttctggcac
      721 aacaaagaag ctggtatgag ttctttatgg acgttttggt ggctggcggt gtgatgaaac
      781 gccttgacct tgccattaac gataagacag ggattttaaa tatccctgta ctcactgaaa
      841 agtgccaaca ggaagaatgt atctccgtct tccgcagttt taaaagctat cgcagtggcg
      901 aactggtacg caaagaggaa aaggaatgta tgggaaacac cctctatatc ggttcattac
      961 aaagtgaagt ttatttctgt atctatgaaa aggactacga gcagtacaag aaaaatgata
     1021 ttcccattga agacgcagaa gtaaaaaacc gttttgagat tcgattgaaa aatgagcgtg
     1081 cctattatgc agtccgtgat ttactcgtct atgacaatcc agagcatacc gcctttaaaa
     1141 ttatcaatcg gtatatccgt tttgtagata aagacgattc caaacctcgt tctgattgga
     1201 aactgaatga agaatgggct tggtttattg ggaacaatcg tgaacgatta aaactaacca
     1261 caaaaccaga gccttactcc ttccaaagga cgctgaactg gctatctcat caagttgccc
     1321 cgaccttaaa ggttgcgatt aaacttgatg aaatcaacca gacgcaggtt gtaaaagaca
     1381 ttctcgacca tgcgaaactg acagaccgac acaagcagat tttgaagcaa cagtcagtaa
     1441 aagaacagga cgtgataaca acaaaaaaag gatatctgtc aaccatacca gttgacagat
     1501 atccaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
     1561 tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac
     1621 ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg tgtataatag
     1681 gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt
     1741 ggtattccaa atgcgtaatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag
     1801 tgattacgca gataaataaa tacgttagat taattcct
//
LOCUS       DAVMHG010000201         1808 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVMHG010000201 DAVMHG010000000
VERSION     DAVMHG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.204"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..903)
                     /locus_tag="VYR08_002030"
                     /pseudo
     CDS             complement(1..903)
                     /locus_tag="VYR08_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1348..1746)
                     /locus_tag="VYR08_002031"
     CDS             complement(1348..1746)
                     /locus_tag="VYR08_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV3467497.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          488 a          362 c          314 g          644 t
ORIGIN      
        1 aggatagttg ttaatccact tttcgatgaa tgcgacttct ttgggagtca ttttcttggt
       61 tcccttaggt aaccatctac gaatgagcct gttgtgattc tcattagttc ccctttccca
      121 agaggcatag ggatgtgcat aataaatgtg ctcctcagaa aatacattag acaagcgatt
      181 gaattccgtt ccattatctg ccgtgatgga aagaatcttg tgttgtttta agatgagttt
      241 tagagcctga ttgaccacct cagcactttt atttggaatc aatcggatga tctgatgtct
      301 actttttcga tccgtcaaga caagcaagca gtagtttttc gctctcgtaa gtagaaccgt
      361 atcaatctca taatgcccat tctccaagcg aaaattgata gcttcaggcc gctgttcgat
      421 ggattgacca gcaggtttaa agttggtgct ggcctgtttc ttaagcgctt ttccttttct
      481 agggtaaagc agatcctgct tgctttaccc caattttcca tgatgaatcc aatagtaaat
      541 ggttgaaatt cccacgttaa cccctttagc cataaccatc atttcaggca aaaatttttg
      601 gttatgatag tggagaatct tttcctttag ttccttggtc acgcttgatc tcttgaccga
      661 gtgcttgcga ttattttcat aagactgttg agcatagtcg gcagaataaa cctctttgaa
      721 gcgccctttt ccaagacatt ttcggactgt cctacgcttg attttagtgt ggatagtttg
      781 aggagctttt ccaagtagag aggcaatttc tctatttgat tttccttctt ttttccatct
      841 ttcgattaag cgacggctat cgattgtcaa atgtttacct tttgtagtat aattgtcttg
      901 catttctgtg cctttcttgt gtttgtggtt gaacaacaag tataacacag aggtgttttc
      961 ttatgcctac aagagctttc attatttcca ttttcttttg gatttcactc tattctgaaa
     1021 aacttgtgta tatttactga agctagcaag tcttacctgt aaatttaatg aaagcaacac
     1081 aaaatccgag aggggaatct cggattaata gatagagagt ttttagttta aataaattgt
     1141 ttaaaatatc aacaacatca cttcttttct taacctgata agtcttgatt cctaattttg
     1201 gggctacgat tatattgtcc tcaatatcgt ctagaaagac acaatttcta ggttataact
     1261 ggtatttatc aatagttact catatacatc agtccacctc catacttatg tgcgagcctc
     1321 tctttgtatt atacctccat actcacctta cagattcttt tggtaataat atctttgcct
     1381 aatgtagaga tagtcttgca aagaaaaaac ttccttgtag ccatgtttct gataaaagtc
     1441 cggtgcctgg aactggtaag tattgacaaa ggcaaaacaa caatttcgat tcttagcttc
     1501 actttctgcc tgttgcaata gttttgaacc gattccttgc cctcgcagtt cctcttttac
     1561 aaacaaatac tcgatttcta gccaatttcc aaaagtctct gctatcaaac ctgccaggag
     1621 attgcccttt tcatcttcga cataaagatt aagtggctca ctttcagcct cttctctttt
     1681 tgaacggtta taaacacgaa tcagattccc tatttcttgc gatttatgtg attccttatt
     1741 ttccaatcta aagtatagtg aaatgaaata aaacatgcgc aaatcgatta aggaatttaa
     1801 tctaattt
//
LOCUS       DAVMHG010000202         1803 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVMHG010000202 DAVMHG010000000
VERSION     DAVMHG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.205"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            192..980
                     /gene="codY"
                     /locus_tag="VYR08_002032"
     CDS             192..980
                     /gene="codY"
                     /locus_tag="VYR08_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV3467498.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            980..1555
                     /locus_tag="VYR08_002033"
     CDS             980..1555
                     /locus_tag="VYR08_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV3467499.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            1637..1722
                     /locus_tag="VYR08_002034"
     tRNA            1637..1722
                     /locus_tag="VYR08_002034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1671..1673,aa:Leu,seq:aag)
BASE COUNT          557 a          295 c          397 g          554 t
ORIGIN      
        1 tattatcttt tataatgtta tgatacaaaa aaataggcaa cttgtcaata gaaaaggaac
       61 aatttaatta aaaatattct ggtttttaat taaattgtaa gtcaatgcaa gtcaataagg
      121 aattttcaga taattattga aaaagagacg tttttatgtt ataatgatag cgattaaatt
      181 cgaggtgaaa aatggcacat ttattagaaa aaactagaaa aattacatca attttgaaac
      241 gctcagagga gcagttgcag gatgagcttc cttacaacgc tattacgcgt cagttagcgg
      301 atattattca ttgcaatgcc tgcattatca atagtaaggg acgtctgctt ggctatttta
      361 tgcgttataa aacaaataca gatcgcgtag agcaattctt ccaaactaag attttcccag
      421 atgactacgt tcaaggggct aatatgattt acgaaacaga agcaaacttg cctgttgagc
      481 atgatatgag tattttccct gttgagagta gagatgattt tccagatggc ttgacgacta
      541 ttgcaccgat tcatgtatcg gggattcgcc ttggttcttt gattatttgg cgtaatgata
      601 aaaaattcga agatgaggac ttggttcttg ttgagattgc cagtaccgtt gttgggattc
      661 agcttcttaa cttccaacgt gaagaagatg agaaaaatat tcgtcgtcgt actgctgtca
      721 ccatggcggt caataccctt tcttactccg aactccgtgc tgtttcagca attttagggg
      781 aattaaatgg aaatgaaggg aagttgactg cgtcagtgat tgcagatcgt atcggaatca
      841 ctcgctctgt gattgtcaat gctcttcgta aacttgagtc tgcggggatt attgaaagtc
      901 gctcacttgg aatgaaggga acctatctta aggtcttgat ttcagatatt tttgaagaag
      961 tgaagaaaag agattactaa tgacaaaggc tttaatttcg attgattata cagaagattt
     1021 tgttgctgat agtgggaaac taacagcagg tgctccagct caggcgattt cggatgccat
     1081 cagcaaggta actcgattag cttttgaacg aggagattat atcttcttta ctattgatgc
     1141 tcatgaagaa aacgattgtt tccatccgga aagtaagtta tttcctcctc ataatctgat
     1201 tgggacgagt ggacggaatt tatatggaga tttggggatc ttttatcaag agcatggttc
     1261 agacagtcgt gtcttttgga tggataaacg ccattactca gctttttcag ggactgacct
     1321 agatattcgt ttgagagagc gtagagtgtc taccgttatc ttaacaggtg tcttgacgga
     1381 tatctgtgtc ctacatacag ctatagatgc ctataatcta ggatatgaca tcgagattgt
     1441 taaaccagct gttgcttcca tctggcctga aaatcatcaa tttgccctag gtcatttcaa
     1501 aaatacactc ggagctaagt tagtagatga aaatctaaat gaactttttg agtaacctgg
     1561 ttggtgaaaa tgaaaaaatt tgaaaaaaag tgttgacaag catcccaaaa gttgatatac
     1621 tagtaaagta atcgacgcgg ggatggcgga attggcagac gcgcaggact aaggatcctg
     1681 tgaccgcttt aggtcgtgag ggttcaagtc cctctctccg cataggaaaa gagctagcta
     1741 aagctagctc ttttgtattt ctctaaaaaa ttagaatcca gtcaaatgta gtggagaatc
     1801 agc
//
LOCUS       DAVMHG010000203         1744 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVMHG010000203 DAVMHG010000000
VERSION     DAVMHG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.206"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..94
                     /locus_tag="VYR08_002035"
     CDS             <1..94
                     /locus_tag="VYR08_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV3467500.1"
                     /translation="SKNKDKTKEKGKRKVKEKVKPNKKDMTREI"
     gene            221..955
                     /locus_tag="VYR08_002036"
     CDS             221..955
                     /locus_tag="VYR08_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV3467501.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEIDKEIEIDKEREGEIDK
                     KSTPIFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILK
                     WYEEDKDKLRQKGLNKKMNYDVGESL"
     gene            1555..1716
                     /locus_tag="VYR08_002037"
     CDS             1555..1716
                     /locus_tag="VYR08_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467502.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          782 a          227 c          323 g          412 t
ORIGIN      
        1 atccaaaaac aaagataaga caaaagaaaa aggaaaaagg aaagtaaaag aaaaagtaaa
       61 accaaacaaa aaagatatga ccagagaaat ctaaagggag taacgaaaag ttacaccctt
      121 aaataaccat aataacaaga cttccgaaaa acagaagtcc agaattccga aaatcggaaa
      181 tcaagaattt cgattatcga aaatcaagaa aggagcagat atggcaacaa acaaaagata
      241 ctattggata aaattaaaag aagaattttt cacagacaaa aggataaaaa ggttaaggag
      301 aatatctgga ggagatacct atacgataat ctacctaaaa cttctactac taagcctaaa
      361 agatgagggc aaactctatt atgacggagt agaaagtgat tttataaaag agttagcact
      421 aaccatagat gaaacagacg atgatgtaat ggtaacagta aattatttaa tcaatcaagg
      481 cttattagaa gttgtaacag aaaatgatga atactaccta actgaaatac caaacttaat
      541 cggatcagaa acagcctggg cagagaaaaa aagaagatac agacaaaata aacagaggac
      601 attgtcctta atgagtccaa cccatgtccg acaagagata gagatagaga tagataaaga
      661 gatagagata gataaagaga gagagggaga gatagataaa aagtccaccc ccatttttta
      721 tggagaattc aaaaatgtaa ggttaagcaa agaagaatat aaaaatctaa aagaaaaatt
      781 aaactcacac acagatataa tgataaataa actatccaga tacatggaaa gcagtggtaa
      841 gacctatcaa aaccactatg tgacaatctt aaaatggtat gaagaagata aagacaaact
      901 aagacagaaa ggtttaaata aaaaaatgaa ttatgatgta ggagaatctt tatagataaa
      961 aagaggtgga aaggcactat taaagacttt aagtctaaaa aggtatcaaa gtatgccaga
     1021 gataaaaaaa tataaatatg acactttaaa ggcgattaga aaattaaaga aatgctagaa
     1081 gacttttaga tgacagttac caagaaagac ttcttttcac tgttaagtat aagagggtaa
     1141 tttatagaat tttgttacat caagataaat attttctgat ggtcaaattt taaaaagatt
     1201 aataactctc gataaatttt gaaccttaac aattgaatag accaagacaa gacgttaatt
     1261 atttttggag cgtaataacc tatccgtcaa gatctttcgg ctgaataatt cggcaaaaaa
     1321 agttctgcta agcaaaaaga aagtgagcga taagtaagag tttgaatata gggagggata
     1381 ccctatccgc tatgaagaaa atgaggataa tgatacttct aaggttgaag ccggctcatc
     1441 cggaatagag gcggaggtga gattcctatg ttgctaatcc aaagcacttg tcaatgtcaa
     1501 aaaacttaat taaacaaata attattagat ataaaacttt agaggaggta tatattggac
     1561 aatgactgga atggattggc tgatttaata gcaaatctga ttgcaaaata tgctggtgct
     1621 ttagatttag ataatctacc cgatccaacg ccagcaaaaa atcaagagat gaaaaatagt
     1681 tttgacatgg caaaaacaca aattgagacg gactaataaa agtgttataa tatacttgaa
     1741 atga
//
LOCUS       DAVMHG010000204         1725 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVMHG010000204 DAVMHG010000000
VERSION     DAVMHG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.207"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..578
                     /locus_tag="VYR08_002038"
     CDS             <1..578
                     /locus_tag="VYR08_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV3467503.1"
                     /translation="TKQDLMEVVDIIQSRYRELPFIDKFTLTPRNSIHMTVIELLCHE
                     NRETEFWSSNLPLDTPLQEIHDYFAKQLEIFPLLDEEIHMRVTEMGKQNILVEPADEA
                     SAKRLEEIRTYVSEKAGVRFPNHDRYQFHISIGYLRIPLTEEEEEEFTKVRAELTEIL
                     LEKIPTITVNRIDYTVFEDMRQFVPYHEKFK"
     gene            603..1670
                     /locus_tag="VYR08_002039"
     CDS             603..1670
                     /locus_tag="VYR08_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV3467504.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLSGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
BASE COUNT          629 a          232 c          339 g          525 t
ORIGIN      
        1 atactaaaca agatttgatg gaagttgttg atattattca atcacgttat agagaattac
       61 catttatcga taagtttact ttaactccaa ggaattctat tcacatgact gtaattgaat
      121 tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag taatcttcct cttgatacac
      181 ctttacagga aatacatgat tactttgcta aacaacttga aatttttcca ttgttggatg
      241 aagaaattca tatgcgtgtg actgaaatgg gaaaacaaaa catactagtt gaacctgcag
      301 atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta tgtctcagaa aaagcaggtg
      361 ttcgtttccc taatcatgat agatatcaat tccatatttc aattgggtat cttcggattc
      421 ctctaaccga agaggaggaa gaagagttta ctaaagtcag agcagaatta actgaaattt
      481 tattagagaa gattccaact attactgtaa accgtattga ctatactgta tttgaagata
      541 tgagacaatt tgttccatat catgaaaaat ttaaataaaa taataaaaaa ggagaactta
      601 tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac gggagttgct cttactacac
      661 ttgctgcttg ctctggaggt tcaaattcta cgactgcttc ttcatctgaa gaaaaagctg
      721 ataaaagtca agaattagta atctattcga actcagtctc aaatggtcgt ggtgattggt
      781 taactgctaa agcaaaagaa gctggtttta atataaaaat ggttgatatc cctggcgctc
      841 aattagcaga ccgtgttatt gccgaaaaga ataatgcagt tgcagatatg gtatttggaa
      901 ttggtgctgt tgattcaaat aaaattagag atcaaaaatt actagtacag tacaagccta
      961 aatggctaga taaaattgat caatctttat cagataaaga taattattat aatcctgtga
     1021 ttgttcaacc attagtttta attggggcgc ctgatgtaaa agaaatgcct aaagattgga
     1081 ctgaattagg tagtaagtat aaaggtaaat attcaatttc tgggctctca ggtggaacag
     1141 gacgggcaat tctagcaagt atcttagttc gataccttga tgataaaggt gaattaggtg
     1201 tttccgaaaa aggttgggaa gtagcaaaag aatatttgaa aaatgcatac actcttcaaa
     1261 agggagaaag ttcaattgtt aagatgttag acaaagaaga tccaatacaa tatggaatga
     1321 tgtggggttc tggagcgtta atcggacaaa aagaacaaaa tgttgaattc aaagttatga
     1381 ctcctgagat tggtgtacca tttgtaactg aacaaactat ggttttaagc actagtaaaa
     1441 aacaagcgtt agctaaagaa tttattgatt ggtttggtca atcagaaatt caagtagaat
     1501 atagtaaaaa atttggatct attcctgcaa ataaagatgc cctaaaagag ttacctgaag
     1561 atacgaaaaa atttgttgat caagtgaaac cacaaaatat tgactgggaa gctgttggaa
     1621 agcatttgga tgaatgggta gaaaaagctg aattagaata cgtaaaataa gaaataagag
     1681 ttatttttta tgtccaagtc tctctattat ttctaaattt aagga
//
LOCUS       DAVMHG010000205         1688 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVMHG010000205 DAVMHG010000000
VERSION     DAVMHG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.208"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..63
                     /locus_tag="VYR08_002040"
     CDS             <1..63
                     /locus_tag="VYR08_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HEV3467505.1"
                     /translation="VVDHFELTIEKWLSKSQQLL"
     gene            complement(578..1570)
                     /locus_tag="VYR08_002041"
     CDS             complement(578..1570)
                     /locus_tag="VYR08_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467506.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          590 a          274 c          214 g          610 t
ORIGIN      
        1 gtagttgacc attttgaact tacaatcgaa aaatggttaa gtaaaagtca acagttacta
       61 tagtttgcaa attatttaca gaaagaacaa aaatgctctg aaacactgat ttcaaagcat
      121 ttttttgtta gtttaaaatt actcccattc ttcttttcca aacgtacaat atatccaaaa
      181 ccattcaaaa tactagattc tattttttat aatatcacta aatccaccta attataggac
      241 gttttcagat ttttagtccc gtcccagtcc cagtaccgga gaaatattgt tttaatataa
      301 tatctctttt tgtcttctaa gctcttaaaa gcaaaagaac aagtaaagag tcaagacaag
      361 gataaaaagt ccatattagg gcaaataaaa agctttaaga cagatgacaa atctaagtca
      421 aataagaaag accatagcaa aggtgcagag agataaatat tggcggtctt cggactgcct
      481 ttattttttt atccattttt caaatcaaat ttattcagac tatatatgca catatacact
      541 taaattcata taaaaacatg gcttgtaaaa aattacttta atcacaataa tcgcatttaa
      601 aattgtgatg tttgcaagct aaattacgga cttcacttgg aagttttccc ttgtatcttt
      661 tataatagat agaaaatttg ctggcagatg aatatccaac agattctgct atctctttta
      721 taggtagttc agtgtttaaa agaagagttt cagctacatt cattcttttt ctttgagtgt
      781 actctgtaat gctttgacaa tatttttcct taaataaatt ttttaattta gtaccactca
      841 ttttagatat tttttcaagc gtgccttgat ttacattcgt tgcaaaatga tcatctaaga
      901 atcttgctac atcttcaagt gctttatcat catcaatttc aattttatat ttttttctat
      961 ttaagtatgt gtcaattact atacttatcc attcatttgc ctttgcttta aagaaaaaat
     1021 cagcggcagg agcgtccatc ttacaattta atatttccat tgccactctt tctaaggcct
     1081 ttgtaagtat tatttgattc ggttgaagca aggttgaata aaaagattct ggattaatgt
     1141 taatagatgc taaatgtttt tctattagct cttttttaaa accaatggaa acagcaagat
     1201 aacaacaatt ctcgtgtaat aaaaaaacaa aatcatcttt tatattatca aaatcaaaag
     1261 tacatagaga gtttgcggta atagtttgat acggattaaa cttttctccg tttgcactga
     1321 caatgtaact tgaataaatt gaaacatagt ctgacatact ataagtgcta ttttgaacta
     1381 cttcctcttt gatataaaaa tcatgtatat cgataatgaa gctgcctcct tcataaaacc
     1441 aatataggcc ttctgcataa gttgaattct cttttttcca acaaaatgta tgacctgcac
     1501 ttgaatactt ctcattgttt ttacattcat ctactttcat ataatctttt acaaaatcat
     1561 aatatgacat aacacactat cccttttaga caatattcca attagcctta ttaattcaaa
     1621 actattgtat tagtaattat aacagatgta taatagaaaa gcaatgatag atattatcaa
     1681 ttaagcga
//
LOCUS       DAVMHG010000206         1644 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVMHG010000206 DAVMHG010000000
VERSION     DAVMHG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.209"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1601)
                     /gene="pfbA"
                     /locus_tag="VYR08_002042"
     CDS             complement(<1..1601)
                     /gene="pfbA"
                     /locus_tag="VYR08_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV3467507.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPIEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNI
                     ELLRVSDNLVVSENSIFGGKEGIVI"
BASE COUNT          503 a          316 c          212 g          613 t
ORIGIN      
        1 tcaataacaa ttccttcttt accaccgaat atactattct ctgagactac taaattatca
       61 ctaactcgta ataattcaat atttggttgt tctgtttcct tagaattatt attaattaca
      121 ttttttgtta cagtaatatc tgatactttt cctaaatatt ctgcactatc ttttgcaact
      181 cgtatcgctt ttgttttagg atcggcaata ttaaatatat tttcggcgat aaccacctgt
      241 ttatttaaat ctaataggtc tttagtgttt ttatagctat aagcatttac taaagctgct
      301 ccgttttctc gataatgtac actctctcct ttaactttct tatcaaagcg atttcctttg
      361 attaatacat cagtgaatcc tgtaaaacgt acacctgcat acatcatgtt atcaaaatga
      421 ttatttaaaa ttttaatatt agaggggttc tgtgtcgaca atgtttgata gtgtgtacca
      481 attgctgtta ctaattcccc agatttatca cttttgccaa aataggaatt ttgaatagtc
      541 acattttcag attttttccc atcatcattc aaggcataag gaaaaccttt tctagttaat
      601 ggttcaatct gaatgctctc cttacttatg atttgcccat ccttcatcgt tttgggtaag
      661 gcttgcccaa gaaaacgaga attatcaact aatacatttt tcgaacctgc aatttgaata
      721 gcatgccctt gataactatc cttgaatgtt acatttttta tagttacgtt atttgaattc
      781 ccaatagcaa atgcacctga agaatttata agtggtagat tttttgcttt agttccttct
      841 tcattcaaag caccgttcat atcaatcgta ccaccagaat aactaatatc ttctattggg
      901 ccccattcta cttgcgcacc atcatccgta aataaacctg tcataaaaac aatggaaggg
      961 tgattcttaa tatttatacc atttagaatt gtagctttct cattcaattc taagtgtgta
     1021 tgacttttta aaaaaacaat ttcttttact aaataagttc cttcaggaaa atatacattt
     1081 cctccaccta gcccttgagc tgcagcatct attgcatctt gaattgcttg tcgatcatca
     1141 tgaatcccat cacctactgc accataatct tttacactta aagcagtatc ttcaaattct
     1201 tttttgataa ttctgtcact tataacatct tccttataac tcttattttc attagtagat
     1261 gttactgtag attcaactgt tctgttgtct aattgtgaat tggaacgaat agtaccatta
     1321 gagctgatac tattaacact taacgaatta tctaaaccat tagtggttga attagaggca
     1381 atcatttcag cagaacgatt ctgattaaca gttggtgaat tatctaaatc attagtaatt
     1441 gcattagaag actcttttgt agccatcggt gaagaactag taacaacttc atccgataaa
     1501 ataccttggc ttcccaaaat tgaaattgcc aatagtaccg agcaagtccc cacctttaat
     1561 ttacgaatac tgaatacctc attaggaaca aaatatttca tcagaacctc caattttttt
     1621 actattttta acaattgatt atta
//
LOCUS       DAVMHG010000207         1572 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVMHG010000207 DAVMHG010000000
VERSION     DAVMHG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.210"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            147..505
                     /locus_tag="VYR08_002043"
                     /pseudo
     CDS             147..505
                     /locus_tag="VYR08_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            562..1572
                     /locus_tag="VYR08_002044"
                     /pseudo
     CDS             562..1572
                     /locus_tag="VYR08_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          577 a          261 c          306 g          428 t
ORIGIN      
        1 ttcgtctgat gggcaaatct tataaagaga ttatagaact tttatagtgg tttgaaataa
       61 gatgtgaaca actctatcag gaaagtcaaa ctaatttata gaaatatttt agcagccaag
      121 gtgtactgtt atagattcaa tacactttag actgtaatca aacaacgatt tggcgaaatg
      181 taaaaaatat gaggagttcg gactcgactc tctccttcaa gaaacacgtg gtggtcgtaa
      241 ccatgcttat atgacggttg agcaagagaa agtctttctt gcccgccatt tgaaggctac
      301 agaggcagga gaatttgtta caattgatgc cttatttcag gcttataaaa aggagttagg
      361 tcgttcctac acacgtgatg ccttctatca actgttgaag cgccatggtt ggcgaaatat
      421 tacgccacgt ccagaacatc ctaggaaagc agatgctcaa accattgtcg cgtctaaaaa
      481 taaagtctca attcaagaag acaagtgaac tgcaccccaa aagttagaca gaaaaaatct
      541 aacttttggg gtgtttttat tatgaaatta acttatgatg ataaagttca gatctatgaa
      601 cttagaaaac aaggatatag cttagagaag ctttcaaata aatttgggat aaacaattct
      661 aatcttaggt acatgattaa attgattgat cgttacggaa tagagttcgt caaaaaagga
      721 aaaaatcgtt actattctcc tgatttaaaa caagaaatga ttcataaagt ctgacatgaa
      781 ggctggacta aagatagagt ttctcttgaa tacggtctcc caagtcgtac gatacttctt
      841 aactggctag cacaatacag gaaaaacggg tatactattg ttgagaaaac aagagggaga
      901 gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat tgaaggagga
      961 aaaagagaaa gaagaaagac agaaattatt caagaattaa tgactgagtt ttcgttagat
     1021 attcttctaa aagccattaa actagctcgt tcgacctact actatcactt gaaacagcta
     1081 gataaaccag ataaggacca agagcttaaa gctgaaattc aatccatttt tatcgaacac
     1141 aagggaaatt atgcttatcg tcggatttat ttagaactaa gaaatcgtgg ttatctggta
     1201 aatcataaaa gagttcaagg cttgataaaa gtactcaatt tacaagctaa aatgcgacag
     1261 aaacgaaaat attcttctca taaaggagac gttggcaaga aggcagagaa tctcattcaa
     1321 ggacaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac agaatttgcc
     1381 attccagcaa gtactcaaaa gctttactta tcaccagttt tagatggctt taacagtgaa
     1441 attattgctt ttaatctttc ttgttcgcct aatttagaac aagtacaaac aatgttggaa
     1501 caggcattca aagagaagca ctacgagaat acgattctcc atagtgacca aggctggcaa
     1561 tatcaacacg at
//
LOCUS       DAVMHG010000208         1552 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVMHG010000208 DAVMHG010000000
VERSION     DAVMHG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.211"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..1133
                     /gene="ugpC"
                     /locus_tag="VYR08_002045"
     CDS             3..1133
                     /gene="ugpC"
                     /locus_tag="VYR08_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV3467508.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
BASE COUNT          487 a          298 c          313 g          454 t
ORIGIN      
        1 tcatggtaga attgaatctt aaaaatattt acaaaaaata tccaaacagc gaacactatt
       61 cagttgaaga tttcaacttg aacatcaaag ataaagaatt tatcgttttc gtaggacctt
      121 caggatgtgg taaatcaact acactccgta tgattgctgg tcttgaagac attacagaag
      181 gtactgcatc tatcgatggc gtagttgtca acgacgtagc tccaaaagac cgtgatatcg
      241 ccatggtatt ccaaaactac gctctttacc cacacatgac tgtttatgac aacatggctt
      301 tcggtttgaa attgcgtaaa tacagcaaag aagacattaa caaacgtgtt caagaagcag
      361 ctgaaatact tggattgaaa gaattcttgg aacgtaaacc agctgacctt tcaggtggtc
      421 aacgtcaacg tgttgccatg gggcgtgcga ttgtccgtga tgcgaaagta ttcttgatgg
      481 acgaaccttt gtcaaacttg gatgccaaac ttcgtgtatc aatgcgtgct gaaatcgcta
      541 aaattcaccg tcgtatcgga gctacaacta tctatgtaac tcacgaccaa acagaagcga
      601 tgacacttgc agaccgtatc gttattatgt cagctactaa gaaccctgct ggtacaggta
      661 ctatcggacg tgtagaacaa atcggtactc ctcaagaagt ttacaaaaat ccagttaaca
      721 aattcgttgc aggattcatc ggaagcccag ctatgaactt catcaccgtg aaattggttg
      781 gtagcgaaat tgtttctgac ggtttccgtt tgaaagtgcc agaaggagca ttgaaagttc
      841 ttcgtgaaaa aggctacgaa ggaaaagaat tgatctttgg tatccgtcca gaagacgtga
      901 atgcagaacc tgctttcctt gaaacattcc cagactgtgt tgtaaaagcg actatctctg
      961 tatcagaact gcttggttca gaatctcacc tttactgcca agttggtaaa gacgagtttg
     1021 ttgcaaaagt tgatgctcgt gactacttgc aaacaggtgc aacagttgag cttggatttg
     1081 acttgaacaa agcacacttc ttcgatgtag aaactgaaaa aacaatctac taaaataaat
     1141 aaaattcaaa gcactacaag aaaagatatc tctttatcaa ttgtagtgga gagatatcag
     1201 ttaatctagg gagagaaaca aaatgcttct ctcctttttg ctagagaagt catattatgc
     1261 atctatattg tgatgctctt taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg
     1321 ccttgccgta cgtatgatta ctgatttcgt cagttttatc tgcaacctca aagatgtact
     1381 ttgagcagct tacggctagt ttcctagttt gctctttgat ttccattgag tattatttgt
     1441 gggtaccatc tacaagtgaa gctatatgcg taaactacgt gagcaattga attcgaacta
     1501 gagaggtaat aataaattta tgctatagtt atggtgactt gtatgctttt ga
//
LOCUS       DAVMHG010000209         1524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVMHG010000209 DAVMHG010000000
VERSION     DAVMHG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.212"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..512)
                     /locus_tag="VYR08_002046"
                     /pseudo
     CDS             complement(1..512)
                     /locus_tag="VYR08_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(588..900)
                     /locus_tag="VYR08_002047"
                     /pseudo
     CDS             complement(588..900)
                     /locus_tag="VYR08_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(919..1305)
                     /locus_tag="VYR08_002048"
     CDS             complement(919..1305)
                     /locus_tag="VYR08_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV3467509.1"
                     /translation="MTYPVEREEITYKRKKAKGKRQAILAQFDSEEVHHRLENCICPD
                     CQGELKEIGASLQRQELVFILAQLKRVNHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL
                     AHSLGSASIIAHTIHQKFILKVPNYR"
     gene            complement(1289..1483)
                     /locus_tag="VYR08_002049"
     CDS             complement(1289..1483)
                     /locus_tag="VYR08_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467510.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
BASE COUNT          407 a          341 c          291 g          485 t
ORIGIN      
        1 actcataata atagctcttg ctttagctcc ttcaaaactt tgagaaaata accaccctcg
       61 cttttttaaa ctgagctcag gctaaaagag tccactggac tcttttactc cgtcccataa
      121 ccaatgattt aatggcgcgt tcagctagat tattggaaag gaccagatgt ccgtctttta
      181 aaatggtctt aaaggtttct ttatacttga ggctgtattc aattgccctt cctagttttg
      241 aacctgctaa aactgactga cggcggcacc aagcaaagaa gtcttccatt aggggctgga
      301 cgtttctgta gtcgttcatc agctggcaaa gcctcccagt ctctttccaa ggcaaataac
      361 tggtcacgat aggctaaacc tttagctcct aaggatgatt tatctgcttg cttgggggtc
      421 gcttcaaaaa atttccttct cacatgcgcc caacatccaa caagttcagc ttcttccagt
      481 tgacgataag cttctagcgg ttcgcagttt accaagctta gcgtcgcctt gctcctaaac
      541 cttagactgc tatcgcatag gcagaactag aggactaaag ttctaagtta ctgccgcaac
      601 atatcacaat gcatatagcc agaataattt cctaggaatt cttgcactac cgaaccactc
      661 cgacgctgat catggtggta cagcgtaatc ccttgtttct cagctttccc agacaaaaaa
      721 gtccaatagt aagtcagctg actatcactt tctaagaccc gataagaggt ttcatctgca
      781 tgaagaagag cttgttctaa caacttttct cgtaaaaggt tataaaggga ctctaaatag
      841 tattgactcg ccttgataat gccaattagc aatttcctta cgtgtgattg gtaaacccat
      901 cttagcccaa tcttcttctt agcgataatt gggtaccttc agaataaact tctgatggat
      961 ggtgtgagcg ataatagaag ctgagccaag gctatgcgcc aaaggggctt taggaatagg
     1021 agctttcacg attttatcac ttggattatt cttactgcat gtttggcatt tgtaagcgtg
     1081 ttggatatga ttcactcttt ttaattgcgc aagaataaag accaattctt gtcgttgaag
     1141 gctcgctcca atctctttta gctctccctg acagtcaggg caaatgcagt tctctaagcg
     1201 atgatgaact tcttctgaat caaattgggc aagaatagct tgacgtttcc ctttagcttt
     1261 cttacgttta taggtgattt cttctctttc aactgggtaa gtcagagtct tcttccatat
     1321 tttgttcctc ttcaaaaaga ctgagttgtc cagatgggca aacacttttc tcagaggatt
     1381 ttccatagag cttttgcgtt agataagcca cttgttcacg aagaagggca agttcattgg
     1441 tgagactatc aattgtagca ctctgttgtt gaatgatttt caatcttctc ccatagactt
     1501 tcctccttat ttcctaactt tatt
//
LOCUS       DAVMHG010000210         1517 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVMHG010000210 DAVMHG010000000
VERSION     DAVMHG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.213"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(86..1510)
                     /locus_tag="VYR08_002050"
     CDS             complement(86..1510)
                     /locus_tag="VYR08_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV3467511.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          438 a          341 c          273 g          465 t
ORIGIN      
        1 accaaaaagt tcgtattttt ctataattat tattatttta tagcaaaaaa ataaaaccag
       61 gaaaacaatt tcctgatttt acacgttatt tcacatcaaa aacaatggat ttgacatttg
      121 tcatcgcttc aatgctatat ttaattcctt gcactccagc tccagaacct ttgacaccaa
      181 ggaatgggaa attatctgga ccacgctggg ttttattatt aatgtggact gtacctactt
      241 caagtttttc agcaatttca aaggcttttt tgaaatcatt tgtaaagact gatgattgaa
      301 ggccgaattc agattcgttg gcaaaggcaa tagcttcctc tacactagcc acacgaatga
      361 ttggtaaaac aggaccaaat ggctcttccc atgccacttt catatctttt gtaacttggt
      421 caaaaagcac tggccagaga agattgccct cacgtttgat tggtgtaaga gcctgagctt
      481 ctttttcttg tgcatcctca atcaagctcc aaatgaagtc ggctgaagca ttgtcaataa
      541 caggtgtaat atcagcattg tcaaatggat caccaactgt taatttagaa acttcttcct
      601 gaagcaaagt agctaattta tctgctacac tttcgagaac aatgacacgt ttaatggccg
      661 tgcaacgttg tcctgagtag ctaaaggctc ccgcaacaat ttgcttggca gcatgttcca
      721 aatctgcatc ttctagtaca agagctgcat ctttcccacc aagttccaac atgataggac
      781 gcataccagc taaacgacca atacgttctc caataggagt tgaacctgta aagttgatga
      841 agttgacttc tttgtgctca atgatataat ccccaatttc tgaaccacga cctgtaatgg
      901 tgttgaaaac acctgccgga atccctgctt cttcaaatgc tttagccaac aagagtccag
      961 aaatggaacc ttgtgttggt ggcttaaaca tgaccacatt ccctgcaatc aaggcaggtg
     1021 caattttaga agcagataaa ttaactggat aattaaaggg agcaatcgct agcacgatac
     1081 caactggttc acgacggaca acagccagtt tgtttttact tgttgcctca aaaccaccac
     1141 cttccattgc ttgtccagtg atacggagac cttcctcagc agcataacga atcaagtctg
     1201 ctgtacgcac tacttctcca attgctgctt taatcccttt tgctacttct ttggcaagga
     1261 tagtaccaat ttcttcctta tcgcgttcta aaatagctgc tgttttatgc aaataagccg
     1321 cacgttcaac tgctgataaa gctcgccatg ctggcagggc tgcacgcgca gcttgcatag
     1381 cctcatcagc ttcagtctga gtcatggctg gaactgtacc caattcttct tgattgattg
     1441 gtgaataaat cgtaatttct tgttcagatg atttccattt tccatttact aaattctgat
     1501 atcttgtcaa atttttg
//
LOCUS       DAVMHG010000211         1472 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVMHG010000211 DAVMHG010000000
VERSION     DAVMHG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.214"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..>1472
                     /locus_tag="VYR08_002051"
     CDS             43..>1472
                     /locus_tag="VYR08_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV3467512.1"
                     /translation="MGKFGVMYTRSKEYGRPSKLFYKSFDSFTEEELFEENECSFRLK
                     IVHIDSNNCFVKSVDFQKGRIFLYSFDRTGFVRHSYTETVAPTPRDIALFSTEKAQYF
                     LGLSSTEEKKDQLILRETSSGDRVSITIPYQDRARRVHCIGRYILLDCSNAANSVFYL
                     ATFKNNSLRELSVNKITFDEKTTLYENSFSDRVLLFLERRTFYEKILSFDLIENTLKT
                     EFERPIIKSNNTKYFSKVIWTKKDSYDVSIPISLFWKSEDTDELPRRKKCILSVYGAY
                     GKNDNSDLDEIMLSIIDAGFIYAIVHVRGGGYLGGEWYRSGKALNKWNSIRDFIEGVN
                     YLRENDVIDSKRLGLITSSAGGIIAGAVLNEEKNLLQSILLFSPFINPYDTLQNPNDP
                     LSKTEIAEWGDIRDPEVKAYIKSYSPMQNIEKARDSNTVIVNILGEKDPYINNNEVIE
                     WSKKLNSIGVKSLLYLNKAAGHGGFTP"
BASE COUNT          508 a          195 c          295 g          474 t
ORIGIN      
        1 ttatttatca ttgaagatat ttcagtagaa agttcgttct atttgggtaa attcggtgtt
       61 atgtatactc gctccaaaga gtacgggagg ccaagtaagt tattctataa atcttttgat
      121 agctttacag aagaggaact gtttgaagaa aacgaatgct cttttcgatt aaaaatagtc
      181 cacattgata gcaataattg ttttgtaaag tcagtagact ttcagaaagg caggattttt
      241 ctatacagtt tcgaccggac gggttttgtt agacactctt acacagaaac ggtagcccca
      301 acacctagag atatcgctct atttagtaca gaaaaagcac agtactttct tgggctctct
      361 tcaactgaag aaaagaaaga tcaactaatt ctaagggaaa cttcttcggg agacagagtt
      421 tctattacta taccatatca agatagggct agaagagttc actgtatagg acgttatatc
      481 cttttagact gttcaaacgc agcgaattcc gtcttttatc ttgctacatt taaaaataat
      541 agtttgaggg agttgagtgt taataagata acatttgatg aaaagacaac actttatgaa
      601 aattcatttt ctgatcgtgt tcttctgttt ttagagagga gaacttttta cgaaaaaata
      661 cttagttttg atttaataga aaatacatta aaaactgaat ttgaaagacc catcataaaa
      721 tcgaataata ctaaatattt ttctaaggtg atttggacta aaaaagacag ttatgatgta
      781 agcataccga tttctttatt ttggaaaagt gaagatacgg atgagctccc aagaagaaaa
      841 aaatgtattc taagtgtcta tggggcttat ggtaagaatg ataattctga tttagatgaa
      901 attatgttat ctataataga tgcaggtttt atttatgcta tagttcatgt aagaggtgga
      961 ggctatttgg gtggtgaatg gtatcgctca ggaaaggcgt taaacaaatg gaattctatc
     1021 agagatttta ttgagggagt aaattattta agggaaaatg atgtaattga cagtaagcga
     1081 ttaggtttaa taacttctag tgcaggtgga ataattgctg gtgcggtgtt gaacgaggaa
     1141 aaaaatttat tgcaaagcat tctcttattt tcgccattta taaatcctta tgatacactt
     1201 cagaatccaa atgatcctct ttctaagacc gaaatagcag agtggggaga tattagagac
     1261 cctgaagtaa aggcttatat aaagtcatat tcgcctatgc aaaatattga aaaggcacga
     1321 gactcaaata ctgttatagt taatattttg ggtgagaaag acccatatat taataataac
     1381 gaagtgatag agtggtcaaa gaaattaaat tctattggag ttaaaagttt gttgtattta
     1441 aacaaagcag ctggtcatgg aggttttact cc
//
LOCUS       DAVMHG010000212         1470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVMHG010000212 DAVMHG010000000
VERSION     DAVMHG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.215"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            254..>1470
                     /locus_tag="VYR08_002052"
     CDS             254..>1470
                     /locus_tag="VYR08_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEV3467513.1"
                     /translation="METRNLISYSLTDIFETDKIRIELLGEIYYKNIKLELHEFAGLY
                     KIYGISLIKNITGMFLIIIFDTKTKELKIFQDITTSYFNLYYTVYGGVFYYSTSLKKV
                     MKLSHVPVTLNNKKIQEFMRNGFILDSNTLVTEINKLEYFSYISVNNTLRICGIDYND
                     SNNYTKEQVLKNWDSMLRESILRVYSEAGEANITLSSGFDSNYILYILANYTNSSINA
                     FCIGGEKGINEIPEVTKIAKFYGINLLVDTIMSQDLEYFPDIVWRLEGSLFECGVILQ
                     YYLGRLLFQKGNTSILCGESADEIMTFKYHSVNYNQFCNDKQKSVYFSYSDYPFYVTN
                     SIVLKKNSLLLHSFGIHPRYPYKMSEIVEMSKKISNLNDKKEFHKKNCELRFQDSVLD
                     NINSVPGTTHLFS"
BASE COUNT          545 a          152 c          243 g          530 t
ORIGIN      
        1 agcgatctta ctaatataga atacttttgt attttttggt gggatagtat gtttgggaaa
       61 gattctattg gaaaatacgt atatgtagaa gagattagta gatgtcattt tttagataga
      121 aattattctt tttgttatgg tttacaagat tataaaagta aacttattaa atatttttta
      181 gaatagttaa tctattcgag agagataaag aaatatggaa aatactttat tgaatatcac
      241 aggaagcttc gatatggaaa ctaggaatct aattagttat tctttaactg atatctttga
      301 aacagataaa ataagaattg aacttcttgg agaaatttat tataaaaata taaagttgga
      361 attgcatgaa ttcgctggat tgtataagat ttatggaatt agcttaataa agaatattac
      421 cggaatgttt ttaattataa tttttgatac gaagacaaaa gaattaaaaa tttttcaaga
      481 tataacgact tcatacttta atttatacta tactgtttat ggaggtgtgt tctattatag
      541 tacatcttta aagaaagtga tgaagttatc tcatgttccc gttaccttaa ataacaaaaa
      601 aatacaagaa tttatgagaa atggatttat tttagattca aatactttag taaccgaaat
      661 taataaatta gaatatttct cttacatttc tgtaaataat actcttcgta tatgtggtat
      721 tgattataat gattcaaaca attatactaa agaacaagta ttaaagaatt gggactctat
      781 gctacgtgaa tctatattaa gagtgtatag tgaagcagga gaggctaata ttacactatc
      841 atctggtttt gattcaaatt atatattata tatattagcg aattatacga attcttcaat
      901 taatgcattt tgcataggag gagaaaaagg aattaatgag attcctgaag taactaaaat
      961 tgcaaaattt tatggtataa atttattagt tgatactata atgagccaag atttagaata
     1021 ttttcccgat atagtatgga gattggaagg aagtctcttt gaatgtggtg tcatactgca
     1081 gtactattta ggcaggttgt tgttccaaaa agggaacaca agtattcttt gtggcgagtc
     1141 agcggatgaa ataatgactt ttaagtatca ttctgtgaac tacaatcaat tttgtaatga
     1201 taaacagaag tcagtatatt ttagttattc cgactatcca ttttatgtta caaattcaat
     1261 tgttttaaag aaaaatagcc tacttttgca tagttttggc attcatccta gatatccata
     1321 taaaatgtct gagattgttg aaatgtctaa aaaaatatca aatctaaacg ataaaaaaga
     1381 atttcacaaa aagaactgtg agttgaggtt tcaagatagt gttcttgata acataaatag
     1441 tgtcccgggt acaactcatt tatttagttg
//
LOCUS       DAVMHG010000213         1466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVMHG010000213 DAVMHG010000000
VERSION     DAVMHG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.216"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..139
                     /locus_tag="VYR08_002053"
     CDS             <1..139
                     /locus_tag="VYR08_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467514.1"
                     /translation="IDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKDEI
                     T"
     gene            453..1466
                     /locus_tag="VYR08_002054"
                     /pseudo
     CDS             453..1466
                     /locus_tag="VYR08_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836677.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
BASE COUNT          687 a          185 c          247 g          347 t
ORIGIN      
        1 tattgattta acaaaagatg aattttataa gttaataaaa acgttaaatg atgaagaaat
       61 aagattaaac ataatggaaa ttttagaaga aagaatagat gataatgtag aaaaatcatc
      121 taaagatgaa ataacataat tgtcccttgg ttacaagggc gcaattatac accctaaagg
      181 gtgcttgcgt cctgcggagt caaacccttg cagggagcaa caaccattcg ctttttaaaa
      241 gtcaagggta aataaactcg ctaaggctcg cccttgactt ttaaaatttg aaatgaaagt
      301 aacaattaag ccgacatact gaaacaacaa aaattaattc agtagtcggc ttttttaatt
      361 ttatcatttc aaaacgctca ttcacaaaga ggatataaaa aaactattaa gcctccacct
      421 aaaacaacaa agaaagaaag gaagtgataa aaatggcaga cagttttcat ttttcagtaa
      481 acataatatc aagaggaaaa ggcaaaagtg cagtagcaag tgcagcatat ataagtggag
      541 aaaaaataaa aaatgaatgg gacggagtaa cccatgacta cacaagaaaa gaaaaaatat
      601 tagtaaaaaa tataatattg cctgatcata taccaaaaga atttaatgat aggtctacct
      661 tatggaataa agtagaaatg gcagaaaaaa attctaatgc acaactagca agacaattca
      721 taataggact accaaaagag ttatctttaa gtgaaaataa aaacctggtt gaaagatata
      781 taaaagaaaa tttaacatca caaggaatga tagtagatta tgcaattcat gatgagagtc
      841 aagataaaaa tggaaatatc cattgtcata taatgaccat aatgcgacca ataaacgaaa
      901 aaggagagtt cttagccaag tcaaaaaaag aatatatcct agatgaaaaa ggagaaaagt
      961 attaaacaaa aatggaaaac caaagacaag aaaagtagaa ctaacaacct ggaacgatac
     1021 aggcaatgta gaaaaatgga gagaaaattt ttcagatctt tgtaacaaat atttagaaag
     1081 agcaggagca gaaaaaagag tagaccatag gagttttaaa agacaaaatt cagattatct
     1141 accgacaatc catttaggat cagcagcaag tgcaatggaa agaaagggaa tagaaactga
     1201 caagggaaac tataatagag aaataagaaa atataatcaa cttgtaaaaa caataaaaga
     1261 agagataaaa acattaaagg gttggatagg aaatttatta gataacttat ctactgctta
     1321 tgaaaaattt aaggatatcg aaagagataa ggtaatagac aaccctaaac tttttaatct
     1381 aacaaattat ctattaactt attcagaaat tcaaaaagaa aaaagtaaat atctaaaagg
     1441 atatgcaaaa actaataaag aaaaat
//
LOCUS       DAVMHG010000214         1459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVMHG010000214 DAVMHG010000000
VERSION     DAVMHG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.217"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            39..779
                     /locus_tag="VYR08_002055"
     CDS             39..779
                     /locus_tag="VYR08_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467515.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            782..>1459
                     /locus_tag="VYR08_002056"
     CDS             782..>1459
                     /locus_tag="VYR08_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467516.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQII"
BASE COUNT          475 a          190 c          291 g          503 t
ORIGIN      
        1 ggaacacttt tcaaaattaa gtgattggag gcaagaatat ggataaagat tatatattaa
       61 aagtgaaagg gctgtatcat caatttttac taggaaataa taaaacgttg caagtgctga
      121 aaaatgtttc tctttctgct tcgagaggag aatttataag tattctagga attagtggtt
      181 ctggaaagtc aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag
      241 taattttaaa tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac
      301 gtaacgaagt atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag
      361 aaaatatagc acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa
      421 acttactcaa aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg
      481 gtggagaaca acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat
      541 ttgctgatga gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat
      601 tgagagagtt agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg
      661 cctcgaaaac tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata
      721 gacctagtgg ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga
      781 gatgctcaga ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc
      841 accagtaatt tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt
      901 tgagaataat tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc
      961 gatagtattc ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt
     1021 tgaaattttt agagatgatt acgaacttct ggaggtgttg ggagcaagtc tccttcaact
     1081 atcatttctt gtggggggac agatttttat catttcctcg attattagtt ttattgcata
     1141 tctatgctct atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga
     1201 aaatatatta cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt
     1261 gttgatttca tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa
     1321 taggaagagt tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc
     1381 tggttttagt gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt
     1441 agcgaaggcc cagataata
//
LOCUS       DAVMHG010000215         1455 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVMHG010000215 DAVMHG010000000
VERSION     DAVMHG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.218"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..559
                     /locus_tag="VYR08_002057"
     CDS             <1..559
                     /locus_tag="VYR08_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467517.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VYR08_002058"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VYR08_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV3467518.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          505 a          208 c          266 g          476 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtga
//
LOCUS       DAVMHG010000216         1453 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVMHG010000216 DAVMHG010000000
VERSION     DAVMHG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.219"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            203..649
                     /gene="rplM"
                     /locus_tag="VYR08_002059"
     CDS             203..649
                     /gene="rplM"
                     /locus_tag="VYR08_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV3467519.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            668..1060
                     /gene="rpsI"
                     /locus_tag="VYR08_002060"
     CDS             668..1060
                     /gene="rpsI"
                     /locus_tag="VYR08_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV3467520.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
BASE COUNT          474 a          286 c          278 g          415 t
ORIGIN      
        1 ttgattatat aactttcagt tactttgaga agttaccgaa aaatattttt catatctatt
       61 gacttttagg ggtaaaattt ggtatgatag taggcggtat tgtttacccc atttgaaagg
      121 ccccggaacc ttccaaatac ttttcgatgg gaaggaacac ccatcaccgt aaacaaaaat
      181 cgaactatat ataggagaaa tcatgaacaa aacaacattt atggctaaac caggccaagt
      241 tgaacgtaaa tggtacgtag ttgacgcaac tgatgtacca cttggacgtc tttctgcagt
      301 agttgctagc gtacttcgcg gaaaaaacaa accaacattt acaccacaca ctgatacagg
      361 tgacttcgtg attgttatca atgctgaaaa agttaaattg actggtaaaa aagcaactga
      421 taaaatctac tacactcact caaaccaccc aggtggattg aaacaaatct ctgcaggtga
      481 acttcgttct aaaaatgcag tacgtttgat tgagaaatca gttaaaggta tgcttccaca
      541 caatacactt ggacgcgctc aaggtatgaa gttgaaagta tttgttggag ctgagcacac
      601 tcacgctgca caacaaccag aagttcttga catttcagga cttatctaag gaaaggaaca
      661 ataaagtatg tcacaagcac aatatgcagg tactggacgt cgtaaaaacg ctgttgcacg
      721 cgttcgcctt gttccaggaa ctggtaaaat cactgttaac aaaaaagatg ttgaagagta
      781 catcccacac gctgaccttc gtcttgtcat caaccaacca ttcgcagtta cttcaactgt
      841 aggttcatac gacgttttcg ttaacgttat aggtggtgga tacgctggtc aatcaggagc
      901 tatccgtcac ggtatcgctc gtgcccttct tcaagtagac ccagacttcc gcgattcatt
      961 gaaacgcgca ggacttctta cacgtgactc acgtaaagtt gaacgtaaga aaccaggtct
     1021 taagaaagct cgtaaagcat cacaatttag taaacgttaa ttcgaaagaa ttactatact
     1081 tatacagagc acctttcggg gtgttctttt ttatactttc ttactaaatt ggtgcaattg
     1141 acacagttgt tgcgacttta gtcgcttaca aatgtggctg caacctgaca aggtcagttg
     1201 cctcaaaacg ttaatcaata cgattatatc aacgtttcaa agcactcaaa agtttacctt
     1261 atttttatag taatgtgaaa taagaatagg acaaaccgag gataatcaaa tcgaattcta
     1321 acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatat tatggataat
     1381 gcaagattcc atagaatggg taagttagaa ctcttgtgtg aagagtttgg gtataaactt
     1441 taacctcttc ctc
//
LOCUS       DAVMHG010000217         1425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVMHG010000217 DAVMHG010000000
VERSION     DAVMHG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.220"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            39..>1425
                     /gene="pcpA"
                     /locus_tag="VYR08_002061"
     CDS             39..>1425
                     /gene="pcpA"
                     /locus_tag="VYR08_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV3467521.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDEKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGV"
BASE COUNT          562 a          196 c          226 g          441 t
ORIGIN      
        1 ttatatctat tagaaaataa atatgtaagg aaatttaaat gaaaaaaact acaatattat
       61 cattaactac agctgcggtt attttagcag catatgtccc taatgaacca atcctagcag
      121 atactcctag ttcggaagta atcaaagaga ctaaagttgg aagtattatt caacaaaata
      181 atatcaaata taaggttcta actgtagaag gtaacatagg aactgttcaa gtgggtaatg
      241 gagttactcc tgtagagttt gaagctggtc aagatgaaaa accattcacg attcctacaa
      301 aaatcacagt aggtgataaa gtatttaccg ttactgaagt agctagtcaa gcttttagtt
      361 attatccaga tgaaacaggt agaattgtct actatcctag ctctattact atcccatcaa
      421 gcataaaaaa aatacaaaaa aaaggcttcc atggaagtaa agctaaaact attatttttg
      481 acaaaggcag tcagctggag aaaattgaag atagagcttt tgatttttct gaattagaag
      541 agattgaatt gcctgcatct ctagaatata ttggaacaag tgcattttct tttagtcaaa
      601 aattgaaaaa gctaaccttt tcctcaagtt caaaattaga attaatatca catgaggctt
      661 ttgctaattt atcaaattta gagaaactaa cattaccaaa atcggttaaa acattaggaa
      721 gtaatctatt tagactcact actagcttaa aacatgttga tgttgaagaa ggaaatgaat
      781 cgtttgcctc agttgatggt gttttgtttt caaaagataa aactcaatta atttattatc
      841 caagtcaaaa aaatgacgaa agttataaaa cgcctaagga gacaaaagaa cttgcatcat
      901 attcgtttaa taaaaattct tacttgaaaa aactcgaatt gaatgaaggt ttagaaaaaa
      961 tcggtacttt tgcatttgcg gatgcgatta aacttgaaga aattagctta ccaaatagtt
     1021 tagaaactat tgaacgttta gccttttacg gtaatttaga attaaaagaa cttatattac
     1081 cagataatgt taaaaatttt ggtaaacacg ttatgaacgg tttaccaaaa ttaaaaagtt
     1141 taacaattgg taataatatc aactcattgc cgtccttctt cctaagtggc gtcttagatt
     1201 cattaaagga aattcatatt aagaataaaa gtacagagtt ttctgtgaaa aaagatacat
     1261 ttgcaattcc tgaaactgtt aagttctatg taacatcaga acatataaaa gatgttctta
     1321 aatcaaattt atctactagt aatgatatca ttgttgaaaa agtagataat ataaaacaag
     1381 aaactgatgt agctaaacct aaaaagaatt ctaatcaggg agtag
//
LOCUS       DAVMHG010000218         1403 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVMHG010000218 DAVMHG010000000
VERSION     DAVMHG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.221"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            77..1255
                     /locus_tag="VYR08_002062"
     CDS             77..1255
                     /locus_tag="VYR08_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV3467522.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          514 a          257 c          202 g          430 t
ORIGIN      
        1 aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga atctgacaac
       61 taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag ctaaaatact
      121 ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta aaacaggaat
      181 aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata ttctcttaga
      241 acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc tagacattca
      301 agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca cttttgcttt
      361 aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta aacagttaat
      421 ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa ataactgctc
      481 tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag ataattataa
      541 aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc aatcccactt
      601 tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta aacgcctatt
      661 tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag gtatgcactg
      721 ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg gagaaatagg
      781 gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg gatgcttaca
      841 aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc atcaatctcc
      901 ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc aagttatttt
      961 aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag ccttactcta
     1021 tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa tctacctaca
     1081 tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa tgaactacaa
     1141 acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca atgattttac
     1201 agccgcacac tcagctattg cactttgttt gtatcataca attctacatt cttaattttc
     1261 tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg aataaacctg
     1321 ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat tatactccta
     1381 tctccccttt ttcataatgt ata
//
LOCUS       DAVMHG010000219         1371 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVMHG010000219 DAVMHG010000000
VERSION     DAVMHG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.222"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..1368
                     /gene="gdhA"
                     /locus_tag="VYR08_002063"
     CDS             22..1368
                     /gene="gdhA"
                     /locus_tag="VYR08_002063"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV3467523.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          403 a          278 c          306 g          384 t
ORIGIN      
        1 tgacaataaa aggagaacga tatgacatct gctaaagaat atatccaaag cgtgtttgaa
       61 actgtgaaag ctcgtaacgg gcacgaggct gaattcctcc aagctgttga agaatttttc
      121 aacactttgg aacctgtatt tgaaaaacac cctgagtata tcgaagaaaa tatcttggca
      181 cgtattactg agcctgagcg cgtggtttct ttccgtgttc cttgggttga ccgtgatgga
      241 aaaattcaag taaaccgtgg ttaccgtgtt caattcaact cagctgtcgg accatacaaa
      301 ggtggacttc gtttccaccc aactgtaaac caagggatct tgaaattcct cggatttgaa
      361 caaatcttta aaaatgtctt gactggactt cctatcggtg gaggtaaagg tggatcagac
      421 ttcgatccta aaggtaaaac agatgctgaa gtgatgcgct tctgccaaag cttcatgact
      481 gaattgcaaa aacacatcgg accatcactt gacgtacctg ctggtgatat cggtgttggt
      541 ggacgtgaaa ttggttacct ttacggtcaa tacaaacgtc ttaaccaatt tgatgctggt
      601 gtcttgactg gtaaacctct tggatttggt ggtagcttga ttcgtccaga agcaactggt
      661 tacggtttgg tttactatac tgaagaaatg ctcaaagcta acggtaacag ctttgctggt
      721 aagaaagtcg ttatttcagg ttctggtaac gttgctcaat atgctcttca aaaagcaact
      781 gaactcggtg caactgttat ttctgtttct gactcaaatg gttatgtcat cgatgaaaat
      841 ggtatcgact tcgatctttt ggttgatgtt aaagaaaaac gtcgtgctcg tttgactgag
      901 tatgcagctg aaaaagcaac tgcaacctac cacgaaggta ctgtatggac ttacgctgga
      961 aactatgaca ttgctcttcc atgtgccact caaaacgaaa tcaacggtga agcagctaaa
     1021 cgtttggttg ctcaaggcgt tatctgtgta tctgaaggtg ccaacatgcc gagcgacctt
     1081 gatgccatca aagtttacaa agaaaatggt atcttctacg gacctgcaaa agctgccaac
     1141 gctggtggtg tagccgtttc agctcttgaa atgagccaaa acagtcttcg cctctcatgg
     1201 actcgtgaag aagttgatgg ccgtctcaaa gacatcatga caaacatctt caacacagct
     1261 aaaacgactt cagaaacata cggtcttgat aaagactacc ttgcaggagc taacattgct
     1321 gcctttgaaa atgtagcaaa tgccatgatt gcacaaggta ttgtttaaga t
//
LOCUS       DAVMHG010000220         1358 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVMHG010000220 DAVMHG010000000
VERSION     DAVMHG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.223"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            5..1228
                     /locus_tag="VYR08_002064"
     CDS             5..1228
                     /locus_tag="VYR08_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV3467524.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
BASE COUNT          483 a          260 c          196 g          419 t
ORIGIN      
        1 acaaatgatt acaaaacaat cagccttttt acaaaataga gccactatac tggagtttct
       61 atatcgaaat ccagcaacat cccgtacaga tattgttaat gaaacaggac ttactccagc
      121 aacaacgact aacatcataa aagagctaag cgaacagtcc ctcatatacg aaactggtga
      181 tgaatttagc gagttttccg gatctggaag acgtagaaaa actatttcta tcacagataa
      241 tattccgtat gttgttggag gtattgaaat aaacgtccta ggtatctttc tcagtctatg
      301 tgacttacaa gggaaaactc ttttcgagac agaaattttg aatgaagatt atcctatttc
      361 agaaatcaat tccaccatta ccaatatgat aaaaacagct atagagtacg tccctttgga
      421 aacaaaatta cttggatttg gcttatcaat acctggacat tataacaaag actccggaag
      481 tatcattaca aacaacccca tatgggaatc ttttaattta ttaaatgtaa ttaaaagatt
      541 caattttcct tttattgtaa aaaataatat cgattgtatg gctataggac aatacctttt
      601 taatccacac aatacccccg ataactttat tttcctacac gctggattag gtatttacac
      661 ttcctttttc acaaaagaaa aaataggagc ctctaaaaat ccttatatcg gagaaattgg
      721 acacaccatt gtcgaattga atgggcaata ttgtgaatgc ggaaaaaaag gttgtttaca
      781 aacatatatt tcggatgctt ggttaatcaa acacgcccaa ttattattta aaaattccca
      841 actaactgta ctaaaaagcc ttgtaaagac tgaaaaagac attcatttag acaccctttt
      901 aacggcttat aatttaggcg actccgcttt acgtcaacaa attgataaag gagtcaattt
      961 attagccact tctattgcaa atctcctcct catcaatcct gctgataaaa tctatatcaa
     1021 cagtcaattg cttaattatc aacctttcac tcatgaagtc agggataaaa tccaagacca
     1081 gctccacttc gttcccttta ctcgtaatat agaaattgaa attttacctt acaacaaaca
     1141 tcgtggaagt ataggagctt gtgcattagc tatcgtcgct tttttcatag aacatagcaa
     1201 tgtattacaa gatattattt caccttaata tattagaaat ctatagacct gtttaaatca
     1261 actataacct gtagtagata tctcgtattt agacaatatg aaaacaagac gacttccata
     1321 taggaaaccg ccttctcgct atgttgagtg atttatat
//
LOCUS       DAVMHG010000221         1356 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVMHG010000221 DAVMHG010000000
VERSION     DAVMHG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.224"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            96..1352
                     /locus_tag="VYR08_002065"
     CDS             96..1352
                     /locus_tag="VYR08_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV3467525.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGL
                     IEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRN
                     FENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT          453 a          291 c          210 g          402 t
ORIGIN      
        1 ccctatggat attatggagc ctatttttgt gtagaaaaaa agtcccataa gatctataat
       61 gaaaagcgat caaacaactc attagaaaga ttcatatgga acaattacat tttatcacaa
      121 aactactcga tatcaaagac acaaatatcc aaattataga tgtcgtcaat agggattcac
      181 acaaagaaat catcgccaaa ctggactacg acgcaccatc ttgccctgat tgtggaagtc
      241 taatgaagaa atatgacttt caaaaaccgt ctaagatccc ttacctcgaa acaactggta
      301 tgccttctag aattctcctt agaaaacgcc gtttcaagtg ctatcactgt tcaaaaatga
      361 tggttgctga gactcccctg gtaaagaaaa atcaccaaat ccctcgtatc atcaaccaaa
      421 aaattgctca aaagttaatt gaaaaaattt ctatgactga tattgcccat cagctggcca
      481 tttcaacttc aactgtcatt cgcaagctca atgactttca ctttgagcat gatttttctc
      541 ggcttccaaa gattatgtct tgggatgagt atgccttcac taagggaaag atgagtttca
      601 ttgcgcaaga ttttgataat cttaatatta tcactgttct tgaaggcaga acacaagctg
      661 tcatccgaaa tcactttctt cgctacgata gagccgttcg ttgtcaagtg aaaatcatta
      721 cgatggatat gtttagtcct tactatgact tggctaaaca gctttttccg tgtgctaaaa
      781 tcgttctaga tcgtttccat attatccaac atctcagccg tgccatgagt cgttttcgtg
      841 ttcaaattat gaatcagttt gaacgaaaat ctcatgaata caaggctatc aagcgttact
      901 ggaaactcat ccaacaggat agtcgtaaac tcagcgataa acgtttttat cgccctactt
      961 ttcgcatgca cttaacaaat aaagaaattc ttgacaagat tttaagctat tcagaagact
     1021 tgaaacacca ctatcagatc tatcaactct tactttttca ctttcagaac aaagaccctg
     1081 agaaattttt cggactcatt gaggacaatc tgaagcaggt tcatcctctt tttcagactg
     1141 tctttaaaac ctttctaaag aacaaagaga aaatcgtcaa cgcccttcaa ctaccctatt
     1201 caaacgccaa attggaagcg accaataatc tcatcaaact tatcaaacgc aatgcctttg
     1261 gttttcgaaa ctttgaaaac ttcaaaaaac ggatttttat cgctctgaac atcaaaaaag
     1321 aaaggacgaa atttgtcctt tctcaagctt agcttt
//
LOCUS       DAVMHG010000222         1353 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVMHG010000222 DAVMHG010000000
VERSION     DAVMHG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.225"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..492
                     /locus_tag="VYR08_002066"
     CDS             <1..492
                     /locus_tag="VYR08_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3467526.1"
                     /translation="ILQILYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLI
                     ISLCFSLIHAQGSTNQANLIGLQHQIPWFSFLLFLINASMVEEFLYREILWNLVRKLD
                     IRVALTSVLFALAHHTGTILAWCLYVSLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSL
                     LLF"
     gene            694..831
                     /locus_tag="VYR08_002067"
     CDS             694..831
                     /locus_tag="VYR08_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV3467527.1"
                     /translation="MAAEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT          394 a          189 c          288 g          482 t
ORIGIN      
        1 atactacaga ttctctatgg ttcgtcactc atttcttttt tatggatgta tggccaacgc
       61 aaacaagcgc ataaggtcaa tatgaaatct aggatgaaat ggcttggtgt tgaatttgtt
      121 agcctactga ttataagtct atgttttagt ctgattcatg ctcaaggaag cacgaatcaa
      181 gcaaacttaa ttggccttca acatcaaatt ccttggtttt catttttatt gttcttaatc
      241 aatgcgagta tggttgaaga atttctgtat cgagaaattt tatggaattt ggtcagaaaa
      301 ttagatatca gagttgcttt gacaagtgtt ttatttgcct tagcacatca tacaggaacc
      361 attctagctt ggtgtttgta tgtttcactt gggatgtttt taggtatggt gcgctataaa
      421 tctgacttat ggggcagtat gggactacat ttggtgtgga acctactagt ttatagtttg
      481 ctgctttttt aaaataggtg acctgatttt taggtaactt ctaaaactaa cttccagttt
      541 tgggagagag atggaagtta ctttgaaaag ttacggaaac attattttct atgtgacaac
      601 attgtataat tgcttgcatt ttactggtgg agagagtata atgagggtgg ataaagtact
      661 taatcccaaa aatttaaata ggaggagcta gctatggcag cagaagtatt aaatcttcaa
      721 ttagtatctg ttcaagttga cgaaacagat gaagttgatg gaatgcgatt tagtacattc
      781 agtacaaacc gttgcggtaa ctggagtgct tttagttggg aaaattgtta attcatttaa
      841 attgtaaact ttagagattc tctggagtta tggatgtcgt tccaatatgt gcacgttgga
      901 atgttagtgc ttatatgggg ggaacagaat cctctcttga ttgaagacaa gctagtcatt
      961 aggctggttt gtctttttgt caactgtagt gggttgatat aatagtatta gtgagtggga
     1021 taaaagtttc atttagttta ttcagtacaa atttaacggg tcaagattta tatactagtg
     1081 gtgtttttgg ggctgagaga agtatcttga ttttatgtgt ggtttttata cttacagttg
     1141 ttctgctcca aagagcttgt agagaagaat tagctcataa aggagattga ttattttgat
     1201 atcaaaaaaa tgcacaggat aacctgatgc atttttttag cgacaatgct tgctacttcc
     1261 ttctgtcgaa tttagacaat tttaaacccc aattattcac cccaaatcta aaaaccatcc
     1321 agaatccttg ccttagctta gatcctggat ggt
//
LOCUS       DAVMHG010000223         1277 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVMHG010000223 DAVMHG010000000
VERSION     DAVMHG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.226"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..595
                     /locus_tag="VYR08_002068"
     CDS             <1..595
                     /locus_tag="VYR08_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV3467528.1"
                     /translation="NTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLENVSGLL
                     GIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSEIREIV
                     SSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQFEKAK
                     SGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            626..829
                     /locus_tag="VYR08_002069"
     CDS             626..829
                     /locus_tag="VYR08_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV3467529.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(940..1212)
                     /locus_tag="VYR08_002070"
                     /pseudo
     CDS             complement(940..1212)
                     /locus_tag="VYR08_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          449 a          211 c          248 g          369 t
ORIGIN      
        1 aaacacaaac actaacgtag aaaagaaaga tgctactgtt gtagctcacg aaatcaaagg
       61 ggaacttact tacgaagata aagttatcca aaaaatcatt ggtctttcac tagaaaacgt
      121 ttcaggtctt ttgggaatcg atggtggttt cttctcaaat cttaaagaaa aaatcgttaa
      181 cagcgatgac gtaacaagtg gtgttaacgt agaagttggt aaaacacaag ttgcagttga
      241 cttaaacgtt attgttgagt accaaaaaaa tgttccagct ttatattcag aaatcagaga
      301 aatcgtatct tcagaagttg ctaaaatgac tgacttggaa attgttgaaa tcaacgtaaa
      361 cgttgtcgac atcaaaacta aagaacagca tgaagcagac tcagtaagcc ttcaagatcg
      421 cgtatctgac gttgctgaat caacaggaga attcacttca gaacaattcg aaaaagctaa
      481 atctggtctt ggatctggtt tctcaactgt tcaagaaaaa gttagcgaag gtgtagaagc
      541 tgttaaaggt gcagcaaatg gtgtagtatc tcacgaaaac actcgtgtaa actaagataa
      601 aataaatata acaggagaaa ttatcatgtc agtagaagaa aaattaaatc aagctaaagg
      661 ttctattaaa gaaggtgttg ggaaagccat cggtgatgaa aaaatggaaa aagaaggagc
      721 agctgaaaaa gttgtttcta aagtaaaaga agttgccgaa gacgctaaag acgctgtaga
      781 aggtgctgta gaaggtgtta aaaacatgtt gagtggcgac gataaataag gttaaaagtt
      841 actttatctt ttttggtaac attagtcaaa agagtctgag tcaagatgat tctcagaaaa
      901 caaaaagcta gagattccca attgcggaac tctagctttt taattttgcc tctttctctt
      961 attatatttc agcaggttgt tggccatgag tatgaatccc atgtcaattc tcacttgacg
     1021 cttacctctc agatgacatc tcttataacc caaacaaacc tttatctgcc caaagacaga
     1081 tttcatatca atcttacgtt tagcgaaaat ttgtctaccc ttggaagata aaagtgcctg
     1141 atattcttta gtttttaaac actggtaacg ttcattcata tacagtccct tttgaggggc
     1201 tgattcaggt tcataatcgc agtcaacatt gatttcaagg ctgtttgctt tctatctccc
     1261 ctttttcata atgtata
//
LOCUS       DAVMHG010000224         1271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVMHG010000224 DAVMHG010000000
VERSION     DAVMHG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.227"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(286..468)
                     /locus_tag="VYR08_002071"
     CDS             complement(286..468)
                     /locus_tag="VYR08_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467530.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(470..1249)
                     /locus_tag="VYR08_002072"
     CDS             complement(470..1249)
                     /locus_tag="VYR08_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467531.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          409 a          278 c          199 g          385 t
ORIGIN      
        1 gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg attttactgc
       61 ttatattata ccatatggaa agagaataaa aaactagcag tggagttcct gctagttctc
      121 tgcaacttgg attaccatct agataagttt aaagagccaa tccaatatct taaacactat
      181 cttgtaaaac agcaattgga ctgcaaaaga gatgatcatc caaaagaatt tcacaatccc
      241 aataatcggt ttgataaaaa gaatagcaag aagaccaaaa aaatttcatt ctctctcctc
      301 tggcttaatg agccaccgag ccgtatccat taacttgtcg gcaataaaaa gtttccccag
      361 accaacaacg acatggtcag ctttctttat cgttttcatc acttttacac cttgcacggt
      421 caaaaagtaa ttaccataaa ttttagcaaa agctttgata ggattcatat tactctcctt
      481 tgatgatttc agcctctttt cggattgttt caacatcttc ttgatattga acttcactat
      541 agttgactag cttggataat tctttctgca agctttcccc cattggtttc atagttgcaa
      601 aggttaaacc acctgaaatg ataccaccca agataggaat aaatttccct attcctttgg
      661 ccagccctcc cttggtaaga ttcacaccaa atattcttaa gacttttttc aaaatagggt
      721 accaaagcgt ctttgttaaa gctttattag gcactgtttt cattacctgt ttggcaattg
      781 ttataccacc agcacgtagc aaagcagcgg ttccattcac ccctaacatt acgcctagat
      841 aaagcaagag ggtattttga gcatcttcac tcaactcctc gcgtgaagcc caaagatcct
      901 cataaccata aatataacct aattcttgag ccaatttcag agagaaagca taaaattgag
      961 ccacatcagc tggaatggta attgccatag caagccctcc agctaatcct gccaaaacag
     1021 aagtcccact cgctaataat acattgtccc gaatacaagc attagctaca cgatctaata
     1081 tttcttgaga tagaagagtc gttgtacctt gttccaataa ggtaggaata tcttttggat
     1141 ccaattcttt ggaaaactta tccactaaaa atttcgaacg atcaacctta acaacaggta
     1201 gtttcaccac ttgttgcaat acttgcatag ctaagtcttg ttcagccata tacaaactcc
     1261 ttatttcttt t
//
LOCUS       DAVMHG010000225         1271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVMHG010000225 DAVMHG010000000
VERSION     DAVMHG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.228"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..722)
                     /locus_tag="VYR08_002073"
     CDS             complement(<1..722)
                     /locus_tag="VYR08_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467532.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     SGIAVFYKESQTTNNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKI"
     gene            complement(1036..>1271)
                     /locus_tag="VYR08_002074"
     CDS             complement(1036..>1271)
                     /locus_tag="VYR08_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467533.1"
                     /translation="DNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEF
                     IACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          459 a          218 c          175 g          419 t
ORIGIN      
        1 tttatttttt taaatcgctt aacttctaat aaatcaattt gttcacgcac gctctctgaa
       61 agatatggct tgatcaaact gatataagat aaagccacgc atggcaactc agattttgat
      121 tgcaatttcc aaatttcatg ataatcctga agattaaact cacaatccct cttagattcc
      181 agtattgtta gtacctttga ttttaacaga aataattgtt gattctcaat tctataaagt
      241 ttttgtacta atgctatgat aatagaaaac ggaataagag gcaaaagcaa cggtttgtca
      301 tacacaaaag taaagaaaaa atagtatgaa attagtgttg taaagtacaa gaagtagtaa
      361 aatttaaaca gattgttagt tgtttgactc tccttataaa acactgctat tcctgaacta
      421 aatcccatta gttggtgaaa gagaattcga tagtatttcc tagctcctat aatgatcaca
      481 aatataaaat aaaaataaat tggtcttaat ttacaaaaga ttgaggaaac tagtaatagc
      541 aaagtatgta agcattgaga caaaatatag aataacggta tcgccaatac aaaggttaag
      601 atggcatgag agggagttcc taagatgtcc aattttataa atactgggct gacacaaaat
      661 atcgtgctta taagcagtaa aattattaaa attgtgtcaa aaaaataacg cttatttttc
      721 attagtctac tttccattgt taaataatac tactgctaac accaagccaa cttagcacat
      781 tgcatcacct aatcctgcgc tacttgtcca taatccttta atgtttctac taaaattatt
      841 ccaatctccc ccatcaagat agcttattta tcctagacaa cgcctactgc aacagtataa
      901 ataagattca tcagccatct tttctggcaa tcatatgggt agcctaatga tatttctagt
      961 tactcaaaaa tatctagcta tactagagtt atttctaatc cctaggacac ttgttaatcc
     1021 aaatgtaaca aattttcacc ccttaatagg actctttctg gaaaaaatat aaggtctatc
     1081 tttataaaaa aatctatcat attgggatgg cacgcatctg gaatttaaac aagctataaa
     1141 ttcatttata actactattt gtttagaagc aacagcatct tgtataccgc cctcgaaaga
     1201 accatcttgt aattccgaaa tatttttgtt taaatattgt tttgacaaat ctcttaatgc
     1261 ttcattatcg t
//
LOCUS       DAVMHG010000226         1259 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVMHG010000226 DAVMHG010000000
VERSION     DAVMHG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.229"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..833
                     /locus_tag="VYR08_002075"
     CDS             105..833
                     /locus_tag="VYR08_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV3467534.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
BASE COUNT          422 a          190 c          210 g          437 t
ORIGIN      
        1 ttgtctagac atttttaaaa attgttgata catatcttgt gtttgcctca tttcttgaac
       61 tttgttatac tactattaga atattataga gagaaccaga ttaaatgagt acaaaatatt
      121 tatttattta caatgagatt cgtgaaaaga ttctttgtaa taaatatacc atgaacgaac
      181 aattacctga tgaaatgaca ttagctaaac agtttgcctg tagtcgaatg acgatcaaaa
      241 aagctttaga cttgttagtt tctgagggct taatttttag aaaacgtggg cagggaacct
      301 ttgttctctc tcgtggcagc tcaaaaagaa aattaatcgt tccagaaaga gatatccggg
      361 gactgacaaa aatatctgaa gatgctcatt ctacaattga ctcgaggatt attcacttca
      421 aattagaatt tgcaaatgaa tttttagcag aaaaactaca ggtcgctttg cagagtccag
      481 tttataatat ttaccgcctg cgtattattg acggtaaacc ttatgttctg gaacaaactt
      541 atatgagtac cgatgttatt ccaggtatta ctgaagatat tttacaaaaa tcgatttaca
      601 attacattga aggaaagtta ggattgcata ttgccagtgc tacaaaaatc ttacgagctt
      661 cttctagttc agaaaatgag caacattact tgcagctcct tccaacggaa ccggtatttg
      721 aagtagaaca agtggcttat ttggataacg gaactccgtt tgagtactcg attagtcgtc
      781 atcgctatga tttatttgaa tttaattctt ttgcattacg acattcctcc taggagaaaa
      841 tgtgaaaatg aagccaatct tttacagact ctagtttaag aaaaatttaa aacagggcaa
      901 gaaggtccca tctatgctta aatggtttct cttttctaaa taagatggct ttaaaagagt
      961 gatcgtttta tccatcatgt tgaaaaatat cttcgtatag cttatagagt aggtactgaa
     1021 attgttcacc tgatctactt cttatagtta tttagtttta aatagtgttt caaatattct
     1081 tacactgacg agaagttttt gagtcttttc ttgtaacaca tatagtatac tgaggttaga
     1141 atagtagact gtgacttcta acaaattgct agaaatgaat ttcaatctcc caatttattt
     1201 gttcaaatat tgttttaata tattgaataa attctgaatc ataatcattt aaaaaaatt
//
LOCUS       DAVMHG010000227         1229 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVMHG010000227 DAVMHG010000000
VERSION     DAVMHG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.230"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..177
                     /locus_tag="VYR08_002076"
     CDS             <1..177
                     /locus_tag="VYR08_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467535.1"
                     /translation="KLEFLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSC
                     NTFYEALLSCSCFN"
     gene            238..1201
                     /locus_tag="VYR08_002077"
                     /pseudo
     CDS             238..1201
                     /locus_tag="VYR08_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
BASE COUNT          378 a          204 c          256 g          391 t
ORIGIN      
        1 aagctagaat ttttatgcga ggagtttggg cataaacttt tacctcttcc tccctactcg
       61 cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct caaaaaggta
      121 ttaccaagtt gcaatacctt ttacgaggct cttttgtctt gttcttgttt caattgacta
      181 tatatcacgg atacagttgg gaaagaagct attaattatt tggagagtcc ttttgggatg
      241 aacatgtcaa atattactat cagatgggca agaaatggat ctgagtcaaa cgatgccttc
      301 ttatgctcaa gtagtgagtc tttatgatga tacaagtatc tctgttagta ataatgagac
      361 ggacaaagtg ttggctggat ccctctatac tgatacaaat gaacagggat taacgattcc
      421 aagcagttta ctaaaaaatt ggaatgaaca gacaggaaaa aatttgacag ctaatgatct
      481 tattggcaaa tcagtctcag ccagcattgt agaaagtgct gccgaaacta gtaagattgc
      541 tcaatttcaa acgaagattg tacgtgtaat caatgatgaa gatgacatgg aggacagcaa
      601 cagtttcatg ctgtctcatc aaatggaaac gattttgaaa gaggctggat ttacgaaagc
      661 tgtatcttat tttatcttgg aactcaaaga tccatcacag acaaaagtag taacagaaga
      721 attacagaaa aataagaagt atactgtgct ttctcaacag agagttcttg atattgtgat
      781 tacctttatt cgtgttattc agggattatt gattgtgctt tcatcacaag ctattgtggt
      841 agcagcggtt atgattggta tcattattta catcaatatc atgcaacgtt ccaaggaaat
      901 aggtgtcatg aaagcagttg gttatcagaa tcgtgatgtc aaaggaattt ttatttacga
      961 ggctatctgg attgtaggca tcgccttgct gctggcattt ttggttgcac aaggggtggg
     1021 aagtttggcg aatgcgattg taagtcactt ttacccatcc atcactaagg tttttgaatt
     1081 aaatctttta tctgttttag gaactctagt tttcgctcta ttacttggtt atgtctcagc
     1141 ctacttcccg gcgcgtaaga ttagtaaaat ggatcctgta gaaccgttac gctatgaata
     1201 gtaagggatt ccttgattta cagtcttaa
//
LOCUS       DAVMHG010000228         1213 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVMHG010000228 DAVMHG010000000
VERSION     DAVMHG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.231"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            128..418
                     /locus_tag="VYR08_002078"
     CDS             128..418
                     /locus_tag="VYR08_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV3467536.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            430..900
                     /gene="ssbA"
                     /locus_tag="VYR08_002079"
     CDS             430..900
                     /gene="ssbA"
                     /locus_tag="VYR08_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV3467537.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            932..1171
                     /locus_tag="VYR08_002080"
     CDS             932..1171
                     /locus_tag="VYR08_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV3467538.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
BASE COUNT          388 a          239 c          251 g          335 t
ORIGIN      
        1 tttttcgaat tctagtgttt ttacttgcgt aaaaaaattt tttctagtat aatagtttat
       61 tgtgagcgaa cctcacttac cccttgcaaa gtcttggggt cattagacca aaaggaggaa
      121 catatcaatg gctaaatacg aaattcttta tatcattcgt ccaaacattg aagaagaagc
      181 taaaaacgct ttggtagcac gttttgactc tattttgact gacaacggtg caactgttgt
      241 tgaatcaaaa acttgggaaa aacgtcgtct tgcatacgaa atccaagatt tccgtgaagg
      301 actttaccac atcgttaacg ttgaagcaaa tgacgatgca gctcttaaag agtttgaccg
      361 tctttcaaaa atcaacgctg acattcttcg tcacatgatc gtcaaaattg acgcgtaaga
      421 aggtaaacta tgattaacaa tgttgtactt gtagggcgta tgacacgtga cgctgagttg
      481 cgttataccc catcaaatgt agcagttgcg acttttactc ttgcagtaaa ccgtacattt
      541 aagagtcaaa atggtgaacg tgaggctgat tttatcaatg tcgttatgtg gcgccaacag
      601 gctgaaaatc ttgctaactg ggctaaaaaa ggctcactta tcggggtgac aggtcgtatc
      661 cagactcgta gttacgataa ccagcaagga caacgtgtct acgtgacaga ggtcgtggct
      721 gagaatttcc aaatgttgga aagccgtagt gtgcgtgagg gtcacacagg tggagcttac
      781 tctgcaccaa ctgcaaacta ttcagcacct acaaattcag taccagactt ttcacgtaat
      841 gaaaatccat ttggagcaac aaacccattg gatatttcag atgatgattt accattctaa
      901 tggacaacta aacttataaa ggagaaaaaa catggctcaa caacgtcgtg gcggattcaa
      961 acgccgtaaa aaagttgatt acatcgcagc aaacaaaatt gaatatgttg attacaaaga
     1021 tactgagctt cttagccgtt tcgtttcaga acgtgggaaa atccttcctc gtcgtgtaac
     1081 aggaacttca gctaaaaacc aacgtaaagt aacaacagct atcaaacgcg ctcgcgtaat
     1141 ggctttgatg cctttcgtaa acgaagatta aagaaaagac ccggttgggt ctctttttca
     1201 tgagcttgaa aac
//
LOCUS       DAVMHG010000229         1158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVMHG010000229 DAVMHG010000000
VERSION     DAVMHG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.232"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            425..607
                     /locus_tag="VYR08_002081"
                     /pseudo
     CDS             425..607
                     /locus_tag="VYR08_002081"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          442 a          205 c          150 g          361 t
ORIGIN      
        1 agatacatac tcctagaata aactttatat gaaattctca tttttgtttt tacaattctc
       61 cttagttaaa tcttgtttaa tatatgtttt acatatagta tttagcgcca catagtactg
      121 aactctctcc aaaaacggtt attcctcttt gaatagggcg ttatcacaag aaaagcatct
      181 ccacgtttca acttcatatg gctcaaaaac aatcaattga tgctaaaacc tgtacctaga
      241 tgtttcggtt cataaaacca tgaaactgta aaagtggatg aaattgatag cgatagtcaa
      301 atcaagaggc atcataactc taaaaagtca caatatataa gttcatcctc ggaaaaatat
      361 cattctaatt gttgaaatgc ctacatgaaa agaaacgtca aatgctcatg aaacaacgaa
      421 tacaggtatc aaaactatga caaaacaagt ccctaaattt actaaagaca ctgctcaact
      481 ttacacctgt aaatggttgt tgtataataa agttacaaag atgtacgacc acactgttgt
      541 aaatcatagt gttcgcgaat atattactga tagcatttct acaaatacaa ttgaagagac
      601 tggatagact ttaaacgaaa tatgtcagtg ttttggcatt cctagccttc atatcattta
      661 aagaattcta tagacaaaat ttttcccaat acagacactc ctaacaactg cttcattttt
      721 ctaccaacat atttaggaac aggataagat acaagaggat taatccatag atcagttcta
      781 taccaatcta agacaaataa gctaaaaaaa cgattgataa taagcaaata gattccaaat
      841 tttctctatc tgctcatttt aataaacaat actagtgtaa ctatccttcc agtcagaagc
      901 ttgtcaaatc acaccgaaaa ttcttctaaa atttatctcg ttaggcaatc aagcaaaaac
      961 tcgacgatag tacaaacatt atcatacaga attgacttcc taaattatat actttagtaa
     1021 ggttttcgta taagaaaaaa gattcatttt acatttctaa acattctttt ctaagatgaa
     1081 aaacagaatt tttcgattgt gatttaaagc aacaagaaga ttttcagtat catcctatag
     1141 atacgagcta attaagaa
//
LOCUS       DAVMHG010000230         1130 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVMHG010000230 DAVMHG010000000
VERSION     DAVMHG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.233"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1130
                     /locus_tag="VYR08_002082"
     CDS             <1..>1130
                     /locus_tag="VYR08_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV3467539.1"
                     /translation="ITLITATLSYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYE
                     LELRKQRYKCKSCHTTFGAITNLTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCS
                     PSSVRRTILERMEPHYRVAKLPKHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLS
                     PTIVDYFESRYSKAERECVQSVVIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRAL
                     DNCRISILKQLDKQSQEYKIMKSHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDL
                     ITSKFSKFAEVYQTYQDITKALNERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYV
                     LNSAKFEYSNGPLEGINRKIKTLKRTCYGFANQKFFFLRIDC"
BASE COUNT          368 a          231 c          183 g          348 t
ORIGIN      
        1 tataacgcta ataacagcta ctctttctta tccgattaat cgttgtcgta actgtggctt
       61 tcccacagtt aataaggatg gctttcgcaa aactcatgta cgactggcaa gtttaaatgg
      121 gagaagatat gaactagagc ttcgtaaaca acgctataaa tgtaaatcat gccatactac
      181 ttttggtgct attactaatt taaccaaaga aaatcaaacc ttatccagtg atctcaaaaa
      241 tcaaatcatg cttttagctc gtaaaggctt atctggtcag cttattgctg aaatgtgtca
      301 ctgctctcct agcagtgttc gtcgaacaat cttagagcgc atggaaccac actatcgtgt
      361 ggctaagttg cctaagcatc tatgttttga cgagtttcgt tcaattaagt ctgtgatgtc
      421 ctttatctgt tgtgacgctg aaacccacca aattgtcaca aagttacagg atcgtctatc
      481 acctaccatt gttgattatt ttgaaagtcg ttattcaaaa gccgaacgcg aatgcgttca
      541 atcagttgta attgatttaa atgctcaata tcaaagtttt atctatcgcc ttttccctaa
      601 tgccaatatc attattgatc gcttccacct tgtacaatta gctggtcgcg ctttggacaa
      661 ttgtcgtatc tctatcctaa agcaacttga taaacagagc caagaatata aaattatgaa
      721 gtcacattgg aagctattcc ataaaaaagc tgaagatctt caccctgaag aagtagtttt
      781 tcttcgcggc gttaaacaat atatgactcg ccaaaatgct gttgatctca ttactagtaa
      841 attttccaag ttcgctgaag tataccaaac ttaccaagat atcacgaaag ccctaaacga
      901 gcgcaatagt gaattactag agtcaaccat cttagactac caaaaaacca atacagaaat
      961 ggatactgct attcaaaccc ttcgtcaaaa cagaaaatat gtcttaaata gcgctaaatt
     1021 tgaatactct aatggtcctt tagaaggcat caatcgcaaa atcaaaaccc taaaacgaac
     1081 ttgttatggt tttgccaatc aaaaattttt ctttttaaga atcgattgta
//
LOCUS       DAVMHG010000231         1112 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVMHG010000231 DAVMHG010000000
VERSION     DAVMHG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.234"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            8..949
                     /gene="prsA"
                     /locus_tag="VYR08_002083"
     CDS             8..949
                     /gene="prsA"
                     /locus_tag="VYR08_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV3467540.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT          432 a          197 c          212 g          271 t
ORIGIN      
        1 agtagacatg aagaaaaaat tattggcagg tgccatcaca ctattatcag tagcaacttt
       61 agcagcttgt tcgaaagggt cagaaggagc agaccttatc agcatgaaag gggatgtcat
      121 cacagaacat caattttatg agcaagtgaa aaacaaccct tcagcccaac aagtcttgtt
      181 aaatatgacc atccaaaaag tttttgaaaa acaatatggc tcagagcttg atgataaaga
      241 ggttgatgat actattgccg aagaaaaaaa acaatatggc gaaaactacc aacgtgtctt
      301 gtcacaagca ggtatgactc ttgaaacacg taaagctcaa attcgtacaa gtaaattagt
      361 tgagttggca gttaagaagg tagcagaagc tgaattgaca gatgaagcct ataagaaagc
      421 ctttgatgag tacactccag atgtaacggc tcaaatcatc cgtcttaata atgaagataa
      481 ggccaaagaa gttctcgaaa aagccaaggc agaaggtgct gattttgctc aattagccaa
      541 agataattca actgatgaaa aaacaaaaga aaatggtgga gaaattacct ttgattctgc
      601 ttcaacagaa gtacctgagc aagtcaaaaa agccgctttc gctttagatg tggatggtgt
      661 ttctgatgtg attacagcaa ctggcacaca agcctacagt agccaatatt acattgtaaa
      721 actcactaag aaaacagaaa aatcatctaa tattgatgac tacaaagaaa aattaaaaac
      781 tgttatcttg actcaaaaac aaaatgattc aacatttgtt caaagcatta tcggaaaaga
      841 attgcaagca gccaatatca aggttaagga ccaagccttc caaaatatct ttacccaata
      901 tatcggtggt ggagattcaa gctcaagcag tagtacatca aacgaatagt ccaaatcaat
      961 gagtcaggga aaaaaactcg acttcaggaa aaaatgaagc aaacattccc acaataaaac
     1021 gcatagtaca aggtttgtac tgaccccaaa agttagacaa ttaatttatc tgaaggattt
     1081 agttctgtat tgcacagggc taagtccttt ta
//
LOCUS       DAVMHG010000232         1103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVMHG010000232 DAVMHG010000000
VERSION     DAVMHG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.235"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..576)
                     /locus_tag="VYR08_002084"
     CDS             complement(<1..576)
                     /locus_tag="VYR08_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467541.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFL"
     gene            complement(573..926)
                     /locus_tag="VYR08_002085"
     CDS             complement(573..926)
                     /locus_tag="VYR08_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV3467542.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGFGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT          401 a          203 c          137 g          362 t
ORIGIN      
        1 aagaaaaaat attctcctct tcattaaagt acttgtgtca aataatctga tgaataaaaa
       61 ttttatcgta gtatctacac aaatttttat caaaaagata agagtcagta catgattatc
      121 aactgtacca taaagaatag atggaaatat agttaatata aaactagaaa tctctaaata
      181 ttttatccaa ggagattgag acaccttgct tctaaactga ctaattttct tatattcgtt
      241 agtcaattta atatcaaaat ctttcctaat taaatgaaag attgctaact ctaggataaa
      301 aacacttatt aatacggtat aagtcaaaaa actttctggg aaaaacttat tgattaaaat
      361 ggggaaactc attaacagaa tcgaatttat ggctagacag atagatacta ttttattttt
      421 cacagaatat agaggtagtg atactattga gtagataaaa gctaaaaaaa taagcaaaaa
      481 atactccgtg tcatttataa tcgccctgaa tgataaaaat atatacatcc aacatacgat
      541 aatgtgtatt tttcgaatct tatctacatt actcatctta ctgtcgcaga tatgaatcca
      601 tcccaaggat aagggttccc gctaatatca caacctctat taagagcacc atatccaata
      661 cagtaagcta ttcttcctgc cgccccagcc aatacacctg ctgctgcacc aataagccag
      721 cctccaaagc ctccaacctt aatatagttt attagttttt tggttaaaac tgcagctcca
      781 gctaatgcta taaaagttcc tggactcata gctaaagctg cgcaaattcc ttggcaagca
      841 cgcttgctta aataagctcc cccatcaaga tacatcattt cttcttcatc aatcacaaca
      901 taattatttg gtaatacaag ttccatttgt tttctcctcc ttagttgaaa ttgtttcatt
      961 tgttttacaa ataggacgaa cttaaactat ctttttatta ctcagtaata attttcgaga
     1021 tttcatttta tgatactctc tattcataca gaaatcaaaa ttttgcctta accaacttgt
     1081 ataatagaaa aaatctaatt gat
//
LOCUS       DAVMHG010000233         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVMHG010000233 DAVMHG010000000
VERSION     DAVMHG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.236"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..402
                     /locus_tag="VYR08_002086"
     CDS             22..402
                     /locus_tag="VYR08_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467543.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            415..558
                     /locus_tag="VYR08_002087"
     CDS             415..558
                     /locus_tag="VYR08_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467544.1"
                     /translation="MAERFWKNLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            651..>1095
                     /locus_tag="VYR08_002088"
     CDS             651..>1095
                     /locus_tag="VYR08_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV3467545.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAH"
BASE COUNT          351 a          165 c          207 g          372 t
ORIGIN      
        1 cgaatctttg ggaggtctcc tatgattgat ctctatctaa gtaaaaatag ccgaagaaat
       61 caacttcttt tagacttctt ccaaaactat ggcatcgagg tatcttgtca ttcagtttct
      121 gaaatgacaa aggacaaatt aattgagatg atgagctatt cttcagattg ttttgagttt
      181 ttatctccaa atttattacg ttttaagaat cgagataacc taaaattaac agatttcatc
      241 gaaatgatat taaaaaatcc tgaactaacc atcagacttc ctcttgcggt ttcaaataag
      301 cgagtttatc caagtctgaa tttggaagag gctagagctc tattgccaag agatacgaaa
      361 caattgattt acatggcgca aacacattat ttatctaact aaaggagaag caatatggct
      421 gaacgatttt ggaagaactt gtccattatt ttggctgaaa gaaatatcag ctggattgag
      481 ttaaccagaa aaatgtttgc gggagagttt cactatccaa gtgaattgaa tcgtttgtat
      541 caaaagattc gccactagaa gatggaacaa cgaatgcctc aaagttcatg ggtagaaagg
      601 attgtgcagg tattagattt agattatgaa gatttatttc ggaggtaact atgaaaagaa
      661 taattccagt ttatatattc caacaagtaa atgtcctatt ggtatctcta tacttattga
      721 aatttctttg tatcggtgag ttaactatac tacaggttct ctatggtgcg tctctttttt
      781 cttttttatg gatgtatggt caacgaaaac aagtggtcaa ggtcaatatg aaaactagga
      841 tgaaatgcct tggtattgga ctagctagtc tactgattat aagtctatgt tttagcctga
      901 ttcatgctca aggaagcacg aatcaaacaa atttaattgg ccttcaacat caagttcctt
      961 ggttttcatt tttattgttc ttaatcaatg cgagtatggt tgaagaattt ctgtatcgtg
     1021 aaattttatg gaacttggtt agaaaattag atattcgagt tgctttgaca agtgttttat
     1081 ttgccttagc acatc
//
LOCUS       DAVMHG010000234         1089 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVMHG010000234 DAVMHG010000000
VERSION     DAVMHG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.237"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..980
                     /locus_tag="VYR08_002089"
     CDS             <1..980
                     /locus_tag="VYR08_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV3467546.1"
                     /translation="FSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVAT
                     IAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNF
                     EAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGME
                     KEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANY
                     LTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLG
                     KINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            970..>1089
                     /locus_tag="VYR08_002090"
     CDS             970..>1089
                     /locus_tag="VYR08_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV3467547.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFL"
BASE COUNT          427 a          153 c          182 g          327 t
ORIGIN      
        1 tttttagcgc ttgttcttct aattctgtta aaaatgaaga aaatacttct aaagagcatg
       61 cgcctgataa aatagtttta gatcatgctt tcggtcaaac tatattagat aaaaaacctg
      121 aaagagttgc aactattgct tggggaaatc atgatgtagc attagcttta ggaatagttc
      181 ctgttggatt ttcaaaagca aattacggtg taagtgctga taaaggagtt ttaccatgga
      241 cagaagaaaa aatcaaagaa ctaaatggta aagctaacct atttgacgat ttggatggac
      301 ttaactttga agcaatatca aattctaaac cagatgttat cttagcaggt tattctggta
      361 taactaaaga agattatgac actctatcaa aaattgctcc tgtagcagca tacaaatcta
      421 aaccttggca aactttatgg agagatatga ttaaaattga ttcaaaagcc ttaggtatgg
      481 aaaaagaagg tgatgagtta atcaaaaata ctgaagctcg tatatccaaa gaattagaaa
      541 aacatccaga aatcaaagga aaaatcaaag gaaaaaaagt attatttact atgattaatg
      601 ctgcagatac atcaaaattc tggatttata ctagcaaaga tccaagagca aattatttaa
      661 cagatttagg tctagttttc cctgaatcat taaaagaatt tgagagtgaa gatagttttg
      721 caaaggaaat ttctgcagaa gaagcaaata agataaatga tgctgatgta atcataactt
      781 atggtgatga taaaactctt gaagctttac aaaaagatcc tcttttaggt aaaataaatg
      841 caattaaaaa tggtgccgtt gctgtaattc cagataatac accgttagca gcctcatgca
      901 ctccaacacc actttcaata aactatacta ttgaagaata tctaaatctt ttaggaaatg
      961 catgcaaaaa tgcgaaataa aaaacaaata aacctaggca taatttttgt aatctgccta
     1021 ggtcttctta ttacaatatt tttgtcatta aagcttggaa caaaagaaat taatatcaga
     1081 gatttttta
//
LOCUS       DAVMHG010000235         1085 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVMHG010000235 DAVMHG010000000
VERSION     DAVMHG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.238"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(170..>1085)
                     /locus_tag="VYR08_002091"
     CDS             complement(170..>1085)
                     /locus_tag="VYR08_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV3467548.1"
                     /translation="RLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEK
                     PAEEPNPAPAPKPEKPADQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKPEQPAP
                     APKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQYNGSWYYLNA
                     NGDMATGWFQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYL
                     NSNGAMVTGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKW
                     YYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT          237 a          230 c          229 g          389 t
ORIGIN      
        1 aaaaaaactc cgtagtctct agcgctataa atccggctag agattacaga gttatttttt
       61 aatctaaact tcctacaagg gtatattatc tactgaaagg atatggtatt tgtagggaat
      121 actttaaaga gttcttacag gaagtcagta ttaactagtt atattaagtt tagtttaccc
      181 attcaccatt ggcattgact ctatagctat ctacagttgt gttgactgca agggcacctg
      241 agccattgac atagtaccat ttatctgata ctttgaacca ttggcttgct ttcatagcac
      301 ctgatgcttc aagatagtac caggtatctc catctttcac ccaatctgtt gccattgaac
      361 cgttagcgtt taggtagtac catgagccat tgttttggag ccatcctgtt accatagcac
      421 cattgctgtt taggtagtac catgaaccat tgtattggag ccatcctgtc gccatatcac
      481 cattagcgtt gaggtagtac catgaaccat tgtattggaa ccatcctgtc gccatatcac
      541 cattagcgtt gaggtagtac catgaaccat tgtattggaa ccatcctgtc gccatatcac
      601 cattagcgtt gaggtagtac catgaaccat tgtattggag ccatcctgtc gccatagcgc
      661 cattgctgtt gaggtagtac catgagccat tgttttggag ccatcctgtc gccattgaac
      721 catcagtatt gtagaagtac cacataccgt tttcttgttt ccagcctgtt tttggtgcag
      781 gagctggttg ctctggtttt ggcggttgct gttgagtcaa gcgattatat tcttcttctg
      841 atctacgagc atagtcttct tcagcttgtt gatcagctgg tttttctgct tttggttgat
      901 cagctggctt ctctggtttt ggagctggag ctggattagg ctcttcagct ggtttttctg
      961 gttgaggagc cggcgctggt tgtggttgtt cagctggttt ttctttaact ttatcttctt
     1021 ctgctgcttt tcgtttagct tcttcttctg cttttttacg atctgtcttg attttttcta
     1081 acctt
//
LOCUS       DAVMHG010000236         1079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVMHG010000236 DAVMHG010000000
VERSION     DAVMHG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.239"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(496..>1079)
                     /locus_tag="VYR08_002092"
     CDS             complement(496..>1079)
                     /locus_tag="VYR08_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467549.1"
                     /translation="NLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIG
                     LEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAK
                     IILKDLGLDEFIERHPMGLSGGQKQRLAIASVMCKTSPFVFFDEPSSGMDYSNMIKIS
                     ELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          363 a          182 c          129 g          405 t
ORIGIN      
        1 atttaccgtg gactaaagtt gtacaagaaa agtgcaaata agaaatctcc agattaggaa
       61 ctatccgtga gttctctagt ctggagattt ttcaatatac ttcgttattg gacggttaca
      121 atttattata tgaaaatccc atatcattct ccaattctat attttacctt tctaaatgta
      181 tagattaacc acctaattat agcatataac gcagattcct ttcaatcgta tgatttactg
      241 cattaaatta agtaaaaaaa taaaggcagt ccgaagactg ccgatattta tctctcatct
      301 ctttaattat ggtaagtaaa taaagaattt ccctaaagat atggaaatta ttaatactat
      361 aaatacatat tataaagttt ataaatactg taaaaatcct gaagttaatt ttctaataaa
      421 tatcaatatg tgttagtatc ttttaaattt ttagacaatt tactagttct atagacatgt
      481 ttaacagact ctattttaca attcaaaaat ttcatctgcc acttcattta aaaattctat
      541 atcatgggaa acaataaaaa ttattttatc catggtttta tacttattaa tcagttcaga
      601 tatttttatc atattggaat aatccatacc acttgaaggt tcgtcaaaaa agacaaatgg
      661 agaagtcttg cacataacag atgctattgc aagcctttgc ttttgccctc ctgataaacc
      721 catcggatgc ctttcaataa attcgtccag gcctaaatct tttaaaatga ttttcgcctt
      781 ttcttcatca aaattcttta ctcctaatct aagctcgttg aatacttcat ctgtgaataa
      841 ttgatgattt acatcttgca taacaagtga agagtttttg agtctttctt ttttagatag
      901 cttctctccc ttaaaataaa tttcttcttt tgattttttc tcaagaccta ttaaacatct
      961 taaaagcgtt gattttcctc gtccgttgga tcctattatg ccataaattt tcccaagctt
     1021 gaatgaaata tcttttaagc ttaaacactc atcatcagta aatttgtagc taagatttt
//
LOCUS       DAVMHG010000237         1062 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVMHG010000237 DAVMHG010000000
VERSION     DAVMHG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.241"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            131..>1062
                     /gene="pspC"
                     /locus_tag="VYR08_002093"
     CDS             131..>1062
                     /gene="pspC"
                     /locus_tag="VYR08_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV3467550.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPSPDTKPSPQPEGKKPSVPDINQEKEKAKLAVVTYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQP"
BASE COUNT          415 a          194 c          208 g          245 t
ORIGIN      
        1 aggctccata atatccatag gggatttacc cactacaaat attatagagc caaataatgt
       61 agcaaatttc atatacaatt catattagca ggatatactg tagttaatta aaattatgga
      121 gaatatagac atgtttaaat caaatcatga gcgaagaatg cgttattcca ttcgtaaatt
      181 tagtgtagga gtagctagcg tagctgttgc cagtcttttt atgggaagtg ttgtacatgc
      241 gacagagaaa gagggaagta cccaagcagc cacttctttt aataggggaa atggaagtca
      301 ggcagaacaa cgtggagaac tcgatttaga acgagataag gcaatgaaag cggtcagtga
      361 atatgtagga aaaatggtga gagatgccta tgtaaaatca gatagaaaac gacataaaaa
      421 tactgtagct ctagttaacc agttgggaaa cattaagaac aggtatttga atgaaatagt
      481 tcattcaacc tcaaaaagcc aactacagga actgatgatg aagagtcaat cagaagtaga
      541 tgaagctgtg tctaaatttg aaaaggactc attttcttcg tcaagttcag gatcctccac
      601 taaaccagaa actccgcagc cggaaaatcc agagcatcaa aaaccaacaa ctccatctcc
      661 ggataccaaa ccaagccctc aaccagaagg caagaaacca agcgtaccag acattaatca
      721 ggaaaaagaa aaagctaagc ttgctgtagt aacctacatg agcaagattt tagatgatat
      781 acaaaaacat catctgcaga aagaaaaaca tcgtcagatt gttgctctta ttaaggagct
      841 tgatgagctt aaaaagcaag ctctttctga aattgataat gtaaatacca aagtagaaat
      901 tgaaaataca gtccacaaga tatttgcaga catggatgca gttgtgacta aattcaaaaa
      961 aggcttaact caggacacac caaaagaacc aggtaacaaa aaaccatctg ctccaaaacc
     1021 aggtatgcaa ccaagtcctc aaccagaggt taaaccgcag cc
//
LOCUS       DAVMHG010000238         1056 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVMHG010000238 DAVMHG010000000
VERSION     DAVMHG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.242"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..140)
                     /locus_tag="VYR08_002094"
     CDS             complement(<1..140)
                     /locus_tag="VYR08_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV3467551.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AG"
     gene            complement(152..718)
                     /gene="amaP"
                     /locus_tag="VYR08_002095"
     CDS             complement(152..718)
                     /gene="amaP"
                     /locus_tag="VYR08_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV3467552.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(802..966)
                     /locus_tag="VYR08_002096"
     CDS             complement(802..966)
                     /locus_tag="VYR08_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV3467553.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          355 a          213 c          152 g          336 t
ORIGIN      
        1 aatccagctg caactcccag tgctcctaaa atcaatacaa atattgtttt gaagaagcca
       61 aaggagacaa tcaaacaagc cagaaatacg cctatgagac cagcgataat tggatatcga
      121 tattgtttaa gccattccat tttttacttc cttacttcac acgactaaca gtctttttgt
      181 tttgaggttt tggttggtaa ttttttactg caacttcaag ttttacttga cgctcaatac
      241 caaaaaactg cttcaatcca ttagttattt cattttgaat tatttggcat ctgtcagcga
      301 tgttgtctga aggaagaatt ttaccttcta catgaacgaa acatttattt tttcgtaaat
      361 ttacatgaac agttgggttc ttgatcaatc tatgatcact caccaaactt ctaacaaaac
      421 cttcgattgc cgaattcttt aattttaatg tatcgttttt agtttcaagt tggatttcca
      481 agtaacgttt aggataaaac atcacaacta aaatggataa taaaactaaa gttgatagaa
      541 ccactgtccc ccaaaagaca tatctagcaa gatagaattc aactacggag ttctgtctcc
      601 agctaagtag atgaatacct agatcactaa cttgatgata atctatcaaa acaggaagga
      661 aaattgtcaa gattaaaata cagaaaataa tgaaacatat tttctttgct tttgacatat
      721 acaatccttt cgatttaaca tttaaactat ttttcccgtc attgatttaa aaatagtttg
      781 ctaactttac tggcgaaaag tttactttct acctgtaaag aatgacacta cagtaatcac
      841 aatcgctgca cctacaatag atgggagcaa agccattcca gcgattgatg gaccccaact
      901 acctaaaaga gattgtcctg catatgcccc gactaaacca gcgaatacat ttgcgatgat
      961 tcccatagaa ctactttttt agtgatttta cctgccaaga gaccaataag acctccagca
     1021 ataattgatc acaacactgc ctaccctcct ttttta
//
LOCUS       DAVMHG010000239         1054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVMHG010000239 DAVMHG010000000
VERSION     DAVMHG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.243"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            98..280
                     /locus_tag="VYR08_002097"
     CDS             98..280
                     /locus_tag="VYR08_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV3467554.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            290..589
                     /locus_tag="VYR08_002098"
                     /pseudo
     CDS             290..589
                     /locus_tag="VYR08_002098"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            653..>1054
                     /locus_tag="VYR08_002099"
     CDS             653..>1054
                     /locus_tag="VYR08_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV3467555.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKS"
BASE COUNT          437 a          130 c          173 g          314 t
ORIGIN      
        1 aggatagaaa tgaccgaatt agaaagaaaa aatcgaaaaa ttagctaaga aatattctga
       61 taacttaaac atcaaagttc aagagagagt tcgtgaaatg gcaaatgata ataagagcca
      121 ttatttgata tacagagttt taggtatttc atttgaagaa ggagaaaata tcgatttgta
      181 tcaaaataaa ggtcgttttt tatacaaata tgctggttca tttttagaag aagctgcagt
      241 actatgcttt aacgaaaaat ttggtacaga aaatacttaa aaagttaaca ttcctaattc
      301 tgaaagtaca aaacctaaga cttttgaaat tgattgttta gtcggaaaaa aacacgcata
      361 cgaaataaaa tggtgggatg caactacaga tggagaccat ataactaaaa aacacactag
      421 aataaaagtt attcataaca aaggatatat accaattcgg ttaatgttct actatccaaa
      481 tagaactcaa gctataaaaa ttcagcaaac tttagaaaca ttgtataacg gtattggagg
      541 gaaatattat tatggagatt ctgcctggga acatttaaga gcagtgaccg gtattgattt
      601 acttagtatt ctaacagata ttgcaaataa aaaaaacagg ggtaaaatca aaatgacagt
      661 attaaaagga gataacttag aaatattaaa aactattgaa tcctcaagta ttgatttaat
      721 ctatatggac cctcctttct ttacacagaa aacccaaaaa ttatctaata acaaaaatat
      781 tatgtattca ttcgaagata cgtggacttc gattgaggat tacaaagaat ttttgtctgt
      841 aagattagaa gaatgcaaaa gagtgctaaa aaatagtggc agtattttcg ttcattgtga
      901 taaaattgca aatcatcata ttagattaat tttagataat atctttggag cagatatgtt
      961 tcaaagcgaa attatatgga actataaacg gtggtctaat tcaaaaaagg gattattgaa
     1021 caatcatcaa aacatttact tttattcaaa gtca
//
LOCUS       DAVMHG010000240         1042 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVMHG010000240 DAVMHG010000000
VERSION     DAVMHG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.244"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..574
                     /locus_tag="VYR08_002100"
     CDS             <1..574
                     /locus_tag="VYR08_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467556.1"
                     /translation="KIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFV
                     MKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            587..757
                     /locus_tag="VYR08_002101"
     CDS             587..757
                     /locus_tag="VYR08_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467557.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          378 a          128 c          200 g          336 t
ORIGIN      
        1 aaaaatacca tcaagattat ataagattga tacaggaaaa gttttatttg atggtgtaaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagcacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc aaaggcaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa gtcagggctg
      421 tactcaaata attgtagctc atagattgtc aacgattaag gatgcggatg ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg atcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa cagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aaaacaataa gaattaagca taattttttg ctgtaaacta
      781 aggagtagag atggctatag ttgaaattat aaatctaaca aaaagcttta aagatattga
      841 agttattcat aacacttaaa taatagagca actacagtag tagcttaaaa acatgattaa
      901 atcgctattc ttaggagtag cggtttttct ttttgtttaa tactctttga aaatctcttc
      961 aaaccacgtc agctttgctt taccgtactc aagtacagcc tgcggctcgc ttcctagttt
     1021 gctctttgat tttcattgag ta
//
LOCUS       DAVMHG010000241         1040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVMHG010000241 DAVMHG010000000
VERSION     DAVMHG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.245"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            623..997
                     /locus_tag="VYR08_002102"
     CDS             623..997
                     /locus_tag="VYR08_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV3467558.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          348 a          129 c          211 g          352 t
ORIGIN      
        1 tagaggtaag accagacttt gttttaatcg agcagaatat tgatgatagt ttaaatagct
       61 cgcaaccatt atctttatct ttagccattt cagaaatcca acttttgctt gaagtgtata
      121 tggggatcaa acatgtttca attaggagat aaaactagaa taacatttgc tattataggg
      181 gcaagttgca tattagtcag tatgtatctt attccactta ttaaaactga gaataaattt
      241 tgggaaatac ttttatttat attaggtgta ttgctaattg gaattgcgtt actaagtcaa
      301 aaaaaataaa ttttgtgtta gtgaatacgt aattattact attgactgtt ttatggtgga
      361 aagcatccat agctttttgt aaaataatct gattggctaa tatttgcagt aggtgttgtc
      421 tatgataaag aaacctatct tgattggatt ttagaactgt taagtttcaa gcaataattg
      481 gttttaaacc ttaggtaacc tataaaaaca aataaggtag agaaaggata tcttatttag
      541 gtaactaagt actataaaca cagtcgacaa tcacttgtta ggaaaccatc ctaactatgc
      601 ttaataaaaa aggagaaaat aaatggaact cgtattacca aataattatg ttgttcttga
      661 gcaagaagag atgatgtatc ttgatggggg attttctatt ccgagatggc ctgttgcaac
      721 agccattaat atagctttta atggtgtttt aggtggagga gcaatcagtc tagttagaaa
      781 ttatattcgt aattatggtt tgcggcgagt tacaagcgca attgctggag cagctgcaag
      841 atatgttggg gtacgagttg caaatagagt ggcaggattt gcactgtctg ctattaatgg
      901 atttgcagct tggatgtcaa ttggcgatgc tattacaaca atctgggcca ataatgatgt
      961 aaataggaga gacccaaatt taaacgcctt gtggtaacta gatatgggta ttaaaatgga
     1021 aaaaattttt gttattattt
//
LOCUS       DAVMHG010000242         1024 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVMHG010000242 DAVMHG010000000
VERSION     DAVMHG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.246"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            161..625
                     /locus_tag="VYR08_002103"
     CDS             161..625
                     /locus_tag="VYR08_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic synthetase"
                     /protein_id="HEV3467559.1"
                     /translation="MAVDKELVAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRN
                     KEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVANNIYSDIDDNFSGQKKLIE
                     QLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLVSIMDNKLTILPQI"
BASE COUNT          344 a          121 c          207 g          352 t
ORIGIN      
        1 aaatccagaa actttttgta aagttggcgc tagtgatgtt cagagtaatg atccttatga
       61 gaattttggt ggattactat atggtcacgc aggggtagct tggctcttcg gtgaagctta
      121 taaattaact ggtgaaagta tctataaaaa tggtctagaa ttggctgtag ataaagaatt
      181 agttgcttat aaagtagata gtaataattc attgcagtac agccaaggtc atagattact
      241 accatatcta gcaactggaa gtgctggcct gcttttactt attaatagaa ataaagaaat
      301 cttgtcaagc aaatacttaa aatatttaac ttctttagag agggcaactg atgtagtttt
      361 ttgtgtatta ccaggtttat ttaacggatt ttgtggtctt gaagttgcta ataatatata
      421 tagtgatatc gatgataact tttcaggaca aaagaaatta attgaacaac tttatagata
      481 tttgtgtgta attgaagaag gatttgttat tgcgggagat aatggtttaa agataacaac
      541 agatattgct tcgggatttg cgggtgttgc aattggattg gtatcaataa tggataataa
      601 attgacgatt cttcctcaaa tataatattt agataccatt attaaggggt catacttgtg
      661 aatcagagta atgaaatatt tttaaataca atacaggaaa aaacacacta taagaggtct
      721 atctcttcat catttcagga attaaaaaaa tggaattctt tgatatttag gagagggcga
      781 tattggtacc aatttgataa ttggagtata cccaatactg gtgtaataaa ggaatggtat
      841 gtcgaagata atgactggat tcctaaggtt agttcttcca gtttagacaa ttcaaattca
      901 cttaaaattg tctttgatgt ggaaaaatat actttgccag atgatagtat cttacaggat
      961 gttaggattt catctgatga gagttatata gttttggttg tatcctcact gcgtagcact
     1021 aatt
//
LOCUS       DAVMHG010000243         1021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVMHG010000243 DAVMHG010000000
VERSION     DAVMHG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.247"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            459..830
                     /locus_tag="VYR08_002104"
     CDS             459..830
                     /locus_tag="VYR08_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV3467560.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            838..>1021
                     /locus_tag="VYR08_002105"
     CDS             838..>1021
                     /locus_tag="VYR08_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467561.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLL"
BASE COUNT          329 a          116 c          212 g          364 t
ORIGIN      
        1 aaagtatgaa taaaaaaata ttttgtattc tagtatgtat tatattattg attagtctgg
       61 ctataatctt tccatggggc tggtcgatat aatgtaaggg agcttgagtt gttagtagag
      121 gataaaatgt taagagtaat agtagttatt caatctttag taataatact gcttgctgtt
      181 gctttattat tatctcggaa aaactttgat tcgttaaatg ttgttcttat aatgatgatc
      241 gtatcacaat tgttgttatt agtatatttg ataaaatcag ggaatcagtg attagctgtt
      301 atttgaatta agtaaactgt taagcttcaa gcagtaactg gttttaaata tttaaaataa
      361 gataaaggaa ggaagtctaa tttaggtaac taaacaaagc ctgcgatcac ttgttaggaa
      421 accatcctaa gcatgcttaa taaaaaagga gaaaataaat ggaacttgta ttaccaaata
      481 attatgttgt gattgatgaa gaagagatga tgtaccttga tgggggggct atatatatac
      541 ccaggtgggc aattacagga gccattactg gtgcagcata tgcagcatta gcagcagcag
      601 gaggtggagg ccttcaacta gttcttgcat cttatggatt acgctccgca ctggtagctg
      661 ggattgttaa aggtttagga gtattaggaa ttcatattgg aaatgctttt gcaaatactg
      721 ttattagaag tattgcatct gctggaattg gtgctggagc tgattggatt tttaccaata
      781 ttattgatgg ctgggatggg cgacgtgata atcaattgag aataggataa taatcttatg
      841 ttgattgttt taatttcagt tattgttgca ttcctgtatc ataaaattac agagtatgtc
      901 aacaacagga aaattgatat tgttattttt ctatcattgc tgatttctca ttttatttgg
      961 aattactatt ttttaccaat tgaaatattg atagcaatca gtggagcaaa tttattatta
     1021 a
//
LOCUS       DAVMHG010000244         1018 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVMHG010000244 DAVMHG010000000
VERSION     DAVMHG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.248"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(244..555)
                     /locus_tag="VYR08_002106"
     CDS             complement(244..555)
                     /locus_tag="VYR08_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467562.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(570..977)
                     /locus_tag="VYR08_002107"
     CDS             complement(570..977)
                     /locus_tag="VYR08_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV3467563.1"
                     /translation="MWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYD
                     DAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIK
                     VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          310 a          198 c          176 g          334 t
ORIGIN      
        1 agacggattg ttttgacaat tctaaccttt tagactttgc ataataactg ttaaacagtc
       61 gaaaattacc ccagttttaa caagtagtca aaataccaga taaaggaaaa aagtctttaa
      121 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      181 ggaagattta cttcattttc tcttggaatt gagttttctc ctacttcaaa actagatgat
      241 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      301 aaaggggcaa cttaaacttt ctttatattg cttatatgtt agtagtttta gacaaacacc
      361 acataaaata ggcttatcct gtatcaaaga taagggatac ggctcaaaag ggtgattttc
      421 taatgaatca tgacaccgat aacaagcata gtacttttta caatcatagc attgtaaagc
      481 aataatatcc aacttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      541 ctgtgcctct atcatttcct tttcctttct taagctcgaa ctgtgacgct ggttccatct
      601 tccttataaa gattgatgag tccttctttt aatgcacgaa ccatgtctcc tgattgaacg
      661 ggttgtggga ctttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      721 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      781 aactcaactt ggtccccttc gttaatgacg tttcgttgac gaatggttgc tgtttgtacc
      841 gcatcatcat aagaaaccac ttcagcgacg aacttgtact caggaatttt acggcgagca
      901 ccaaacaact gctcattttc agatggtata ccgtagtaga aacctgttgc caattcacgt
      961 tgggcaacct tccacatctc gtccaccaag tcttgtttga tagcttcaaa cttttcag
//
LOCUS       DAVMHG010000245         1018 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVMHG010000245 DAVMHG010000000
VERSION     DAVMHG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.249"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..156
                     /locus_tag="VYR08_002108"
     CDS             <1..156
                     /locus_tag="VYR08_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467564.1"
                     /translation="LWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVN
                     ANGEWVG"
BASE COUNT          347 a          125 c          179 g          367 t
ORIGIN      
        1 ttatggtatt acttaaatga atcaggttca atggctactg gttggtttac agtttctggt
       61 aaatggtact atacctataa ttcaggagat ttattagtaa acacgactac acccgatggc
      121 tatcgagtca atgctaacgg tgagtgggta ggataggtat ccattagcta aatctaatca
      181 ttctgaacta aaaaatcttc ttacatatgt aggaagattt tttggattct caaaactagt
      241 ttaaaagact acgctattta aaccaatttt aagaatggat atataaggat attcagaaat
      301 ctagttggtc aacttttgta ttttgaatat accttagatg gtgacgatgt cttggtatat
      361 ctggtactca cttacatatt ctaacttatt ttaactaaga tacaactgta gttggtgaat
      421 ttgctacaaa tagtgtagag gcgttatttg aaaaatatac aaaaaaggaa ccccaactta
      481 catttgagtt cctcttgctt ttaattgtcc gaatccgtat tcttatttca aaaaaggtag
      541 tcattcgaat gatttccctt gcaatatagt gaaattaaca aaaagaaaaa acgctgatca
      601 agcgtttttc ttctgtattg attcgtattg aatgcttact tcacttctgt gaagaagatg
      661 ttgttttaaa ggtatgtaat gatatttgaa aaccttgatt tgatagcttt tctacctaaa
      721 ataaaataaa tggtaaatag agaaatatga atttattgaa atttaatcag ttaggaatca
      781 gttacgataa attaatattc tttggaaatc agaatatagc acttattagt attgctttat
      841 cataggttag atattttctt aattagataa gggcgttgta gtttcaattt atggagtaca
      901 ttagtttgta gaagtattaa acaaaattac tcgttatgat agttcatcaa aggtattata
      961 tcagatgtac aaagtgtgta gagaagtttt tggataggaa ttacttgaaa agtatgtg
//
LOCUS       DAVMHG010000246         1012 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVMHG010000246 DAVMHG010000000
VERSION     DAVMHG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.250"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>1012)
                     /locus_tag="VYR08_002109"
     rRNA            complement(<1..>1012)
                     /locus_tag="VYR08_002109"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          221 a          320 c          210 g          261 t
ORIGIN      
        1 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
       61 gcaccacctg tcacctctgt cccgaaggaa aactctatct ctagagcggt cagagggatg
      121 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      181 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      241 cttaatgcgt tagctacggc actaaacccc ggaaagggtc taacacctag cactcatcgt
      301 ttacagcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      361 cgtcagttac aagccagaga gccgctttcg ccaccggtgt tcctccatat atctacgcat
      421 ttcaccgcta cacatggaat tccactctcc cctcttgcac tcaagttaaa cagtttccaa
      481 agcctactat ggttaagcca cagcctttaa cttcagactt atctaaccgc ctgcgctcgc
      541 tttacgccca ataaatccgg acaacgctcg ggacctacgt attaccgcgg ctgctggcac
      601 gtagttagcc gtccctttct ggtaagatac cgtcacagtg tgaactttcc actctcacac
      661 tcgttcttct cttacaacag agctttacga tccgaaaacc ttcttcactc acgcggcgtt
      721 gctcggtcag acttccgtcc attgccgaag attccctact gctgcctccc gtaggagtct
      781 gggccgtgtc tcagtcccag tgtggccgat caccctctca ggtcggctat gtatcgtcgc
      841 cttggtgagc cgttacccca ccaactagct aatacaacgc aggtccatct ggtagtgatg
      901 caagtgcacc ttttaagcaa atgtcatgca acatctactc ttatgcggta ttagctatcg
      961 tttccaatag ttatcccccg ctaccaggca ggttacctac gcgttactca cc
//
LOCUS       DAVMHG010000247          999 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVMHG010000247 DAVMHG010000000
VERSION     DAVMHG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.251"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..384
                     /locus_tag="VYR08_002110"
     rRNA            <1..384
                     /locus_tag="VYR08_002110"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            436..508
                     /locus_tag="VYR08_002111"
     tRNA            436..508
                     /locus_tag="VYR08_002111"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Ala,seq:tgc)
     gene            633..>999
                     /locus_tag="VYR08_002112"
     rRNA            633..>999
                     /locus_tag="VYR08_002112"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          206 c          288 g          212 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc
      301 cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga
      361 aggtgcggct ggatcacctc ctttctaagg ataaggaact gcgcattggt cttgtttagt
      421 cttgagaggt cttgtggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
      481 gtcagcggtt cgatcccgct aggctccatt ggtgagagat caccaagtaa tgcacattga
      541 aaattgaata tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa
      601 taagagttta tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga
      661 tgccttggca ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg
      721 taagtaagcg atgatccagg gatttccgaa tgggggaacc caacaggtaa tacctgttac
      781 ccacatctgt taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc
      841 aggaagagaa agcaaaagcg attgccttag tagcggcgag cgaaacggca gaagggcaaa
      901 ccgaagagtt tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga
      961 atgatttggg aagatcagcc aaagagagta atagcctcg
//
LOCUS       DAVMHG010000248          979 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVMHG010000248 DAVMHG010000000
VERSION     DAVMHG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.252"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          383 a          187 c          104 g          305 t
ORIGIN      
        1 taaatataaa agtaaaaccg ccatcaatat acttacaaaa atccccttgg actcaatcaa
       61 tggaaaataa aatattgcaa ctagaagcga ataaagtccc gctaataaat atttctgatc
      121 atctttcatt caaaacacca tattatttac taagaataca tcaatacaat agagcaactc
      181 tgtaagtcaa tatttgataa agcaaagctt taaccccttc cccagacgtt agcttttctt
      241 caacccacta cagttgacaa ggagcccaaa ttattgataa aaactttagt catcatcgtc
      301 tctataaaaa tattttctat acactcttga taataccaaa attaatacca atatcatacc
      361 aagagctata ataatagaat ttattagatt tattttagta tatccaatca ctagtccaat
      421 aattggacta gcagacatgc ttagatataa taaaaataac aaaaaatgaa tttcaccaat
      481 tcctgcaaaa aaatgtttca tagctataat tttacgccca taaataccca tcaaatttac
      541 cgtctacgat atctaaacca tatgcaatgg ctcctcctaa tgagaagact tcagcaacag
      601 tagccgcaat attaagagca ttagtaatag ttgccatact aactccacca atatatcttg
      661 ttatagcact tagttaccta gataagatat cctttagctt gcttccaaaa tttaagtctg
      721 cacctataat atgagttcat attagtactg ctaaatccta tctatagtaa tgtcaaacac
      781 ctaaataaag tcatctctcc gattcattca agaaatgaag aaaggcaaaa aatctttaaa
      841 ataagaaaag tgcatattat caggtgttca aaaatattga tgatatacgt tatatagata
      901 gaaagattta ctcaatctca ctttcaaatt gagttttttc ccaacctcgt ttttaattta
      961 tatcttcaat aaaatctaa
//
LOCUS       DAVMHG010000249          978 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVMHG010000249 DAVMHG010000000
VERSION     DAVMHG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.253"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..737
                     /locus_tag="VYR08_002113"
                     /pseudo
     CDS             1..737
                     /locus_tag="VYR08_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(772..>978)
                     /locus_tag="VYR08_002114"
     CDS             complement(772..>978)
                     /locus_tag="VYR08_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467565.1"
                     /translation="MDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIK
                     KNLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT          320 a          208 c          160 g          290 t
ORIGIN      
        1 gctgagactt ctatcgtcaa gaagaatcat caaattcctt gtatcatcaa ccaaaagatt
       61 gctcagaagc taattgaaaa gacttctatg accgacattg atcatcagtt gtctatttca
      121 acttcaactg tcattcgcaa gatcaataat tttcactttg agcatgattt ttcgcgtctt
      181 cctgagatta tgtcctagga cgttgaaaca gtccggggag tgactgtttc aatcggggat
      241 ggagatgagc tttattgctc aggactttga taaactcaat atcataactg ttcttgagag
      301 cagaacacaa gccatcatcc gaaatcccat gaatacaagg ctatcaagcg ctactggaag
      361 ctcattcaac aagatagtca gaaactgagt gataaacgat tttatcgccc tacttttcgt
      421 atgcacttga ctaataaaga aattctagat aaacttttga gctattccga agacttgaaa
      481 caccactata atctctatca gctcttgctt ttccacctct gtttcaaact ttgtttaaaa
      541 ccttcctcaa agataaagag aaaatcgtca acgcccttca attaccttat tccaacgcca
      601 aactggaagc caccaataat ctcatcaaac ttatcaagca caatgccttt ggttttagga
      661 actttgaaaa cttcaaaaaa gaaaggacga aatttgtcct ttctagatct tcgctttctt
      721 caacccacta cagttgacaa agaaccgaaa aatgtctcgc ctctaatata gtcaattgaa
      781 acaagaacaa gagaaaagag cctcgtaaaa ggtattgcaa cttggtaata cctttttgag
      841 gttctttttg atataagccc atgttttctc aataggattg tactcaggtg agtagggagg
      901 aagaggtaaa agtttatgcc caaactcctc gcataaaagt tctaacttac ccattctatg
      961 gaatcttaca ttatccat
//
LOCUS       DAVMHG010000250          974 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVMHG010000250 DAVMHG010000000
VERSION     DAVMHG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.254"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..101)
                     /locus_tag="VYR08_002115"
     CDS             complement(<1..101)
                     /locus_tag="VYR08_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV3467566.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSV"
     gene            complement(114..862)
                     /locus_tag="VYR08_002116"
                     /pseudo
     CDS             complement(114..862)
                     /locus_tag="VYR08_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
BASE COUNT          341 a          190 c          109 g          334 t
ORIGIN      
        1 tttacagaat ctatatcata tgcaacaact ttatatttag atgcaaatag taacgcgtag
       61 gccagcccaa catgccccaa accaattact gctatattca taaaactact tccttatttc
      121 ttaatccaaa atctaataga ataagctgcc ccattcctta aatacaactc tttaatattg
      181 tttaaaagtt tttcaactga tttccagatt atcaaaatct gagatttata gcacaatatt
      241 gatgatattc tatcaatata attttttcat caagttcctc ttgatacatt tttaattctt
      301 tagtttttcc catataacta accatactac tatcacttac atatgggaag tcctcataat
      361 atattacttt ataacgcata aattcaagcg cccttccaat actattcaca aaaacatgag
      421 caacatggtc accaagtgaa agcggacaat atacgacaca tttgtcgtct aaatgcatta
      481 acagctcttt tatgatatca ttctttaatg tgtcctcatt ttttaattca ctatagatat
      541 gacggtatag aaaattgcca tttctatctt tcctatagag acattcatag tacgataagt
      601 gtctaaaatc acattgtaga cgttcacaag ctaacctgtc ttctttcttc ctttcttcaa
      661 tcggatattt cccaaggtta cacaacttat gaaattgctt agcagagggc tgtagctgtt
      721 ggctcaaagg gtaaccagaa aatatagtaa taacaagtac aatttctcct tctgaagtta
      781 attttgaaat ataatcacca caggaaaaaa ttgcgtcatc taaatgtgga gataaaaaga
      841 tatacttagt attgttactc ataaccattc cctctacaat ttatctaaaa actcactaag
      901 tgtctgatta aattccacat catcaaaaaa attcacctta ttcttaataa tgaatatttc
      961 gttaaataaa cata
//
LOCUS       DAVMHG010000251          885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVMHG010000251 DAVMHG010000000
VERSION     DAVMHG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.255"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            16..315
                     /locus_tag="VYR08_002117"
     CDS             16..315
                     /locus_tag="VYR08_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467567.1"
                     /translation="MQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKSYDEKSHEE
                     LVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVVEKYGH"
     gene            305..>885
                     /locus_tag="VYR08_002118"
     CDS             305..>885
                     /locus_tag="VYR08_002118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV3467568.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYN"
BASE COUNT          357 a          110 c          163 g          255 t
ORIGIN      
        1 tctgtatttt cgacaatgca agatattgag aatgttgcaa ccgatattat aaaatcatat
       61 gataatgaga tttatactta taaagctgtt tcccaagaag aattggaaaa actagaaaaa
      121 agttatgatg aaaaaagtca cgaagaatta gtttcaatag aaagcaattt agaaatgaaa
      181 caacagaacc ttattgatga ggttaataaa acaatcaagg aaaatgatgc aaatattcag
      241 tatatttcat caagtaggag aggagaattt gtagaaaaaa ttattggtag ggtggtagaa
      301 aaatatggcc attagtcaga tgaaaagaat ctctctacta ttttctaaaa gtagtcttga
      361 tgatgtttta aaaactattc aagaactaga gtcagtgcag ttccgtgatt taaaggttca
      421 ggataactgg tcagaagctc tagaaaaaga tgaagttgta tttccaacta ttcaaatttt
      481 tcatacttct aattccaatc atggggttat tgagggaaat gatgccttga cttatttgat
      541 gaatcaacaa caacatttag aagcaactgt agagaaatta caagaatacc taccgaaaga
      601 aaacacgttt aaattattgc agcaacctcc gataactacc tcttatgaag aattagagaa
      661 atttggtaaa gctaatgttg ctgagggtgt tcttaaaaaa gtgaatcatc aaattaacag
      721 agttcatgaa ttagaaagac acattcaaag taataatgag gaaatagagc gattaataaa
      781 gtgggaaaaa ttagaaattg ttcctgcgaa tttagaacaa ttttctttct gtaaaggaaa
      841 agtcggaaca attccaagga ctgaagataa tcgcttatac aatag
//
LOCUS       DAVMHG010000252          879 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVMHG010000252 DAVMHG010000000
VERSION     DAVMHG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.256"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..264
                     /locus_tag="VYR08_002119"
     CDS             <1..264
                     /locus_tag="VYR08_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467569.1"
                     /translation="IDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAKDNAGNW
                     TSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            261..407
                     /locus_tag="VYR08_002120"
     CDS             261..407
                     /locus_tag="VYR08_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467570.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            550..720
                     /locus_tag="VYR08_002121"
                     /pseudo
     CDS             550..720
                     /locus_tag="VYR08_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          329 a          133 c          171 g          246 t
ORIGIN      
        1 attgataaaa cgtctgatgc aacagctctt ttgaaattaa agttgaataa tgttgataat
       61 caacctgcta ctgaagttcc tagttcagga attactgtaa aattaaatgc taaagataat
      121 gctggaaact ggacaagtgc ttcgaataaa aaagaaataa cagtaaaaat tgtttctgct
      181 aaaccgacat atccagacaa aatcttagtg aaaaatcctg ataatataaa agatacagaa
      241 aaaatgccat tattgaaaaa ttgaaagagg caaataaaaa tcatccagca ggagctccaa
      301 cctttgctaa aggtgaagga gagcatgcaa atgatattgt agcaacttat tcagatggta
      361 caacttatta tgtaccgtta aatgatgtga caaaatatgc gaggtagtgg ctgtaccact
      421 cacttattca cctccccgtg atttgtagta gtgataggct ttctcactat tattataaaa
      481 caaaataaag atcacaacac tttttcattc tgtgttgtgc cttgagtgaa acgaaaggaa
      541 tgaattataa atatgaaaag tatagtcact agcatagatg agcgcttgcg tactcgccta
      601 cgagtgatta tctggaagca atggaagaag aaatcgagac gattatgggg attgcttaag
      661 ttaggggttc ctaaatggat agcagataag gtatctggct ggggcgacca ctatcaatta
      721 tattaaaatg aaataagaat aggacgaatc gatcagggaa gtcaaatcga tttctaacaa
      781 tattttagaa gtagaggtat attattctag tttcaatcta ctatagtagc tcagaagtcg
      841 gtacttaaac gtgctatatc aaaaccagtc cttggaaaa
//
LOCUS       DAVMHG010000253          873 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVMHG010000253 DAVMHG010000000
VERSION     DAVMHG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.257"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            94..609
                     /locus_tag="VYR08_002122"
     CDS             94..609
                     /locus_tag="VYR08_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467571.1"
                     /translation="MNRLKELRQEKKLSQKELAENIGVHYRTLQNWENGESQIKPEKA
                     QQLADYFGVSVGYLLGYSEYRELEKALDKTIFSNYPDVETFLTQEIKELIGERTKDFY
                     EYIDKQFYESYKNTTVPPEIVVKHREDFYSSFLFLPARLQKFIALWSILTETEQENIG
                     KTIELLAMRGR"
BASE COUNT          336 a          141 c          138 g          258 t
ORIGIN      
        1 tctattgtca actatttttt taatttttgc acaaaaaaaa tgtacttttt ctaagatata
       61 gtatataatc atctgtgaaa ggtgtgataa aacatgaata gattgaaaga attaagacaa
      121 gaaaaaaagc tatctcaaaa agagttagct gaaaatattg gggttcatta tcgtactctt
      181 caaaattggg aaaacggaga aagccaaatc aaaccagaaa aagcccaaca actagctgac
      241 tacttcggag taagcgttgg atatctgttg gggtatagtg agtatagaga attagaaaaa
      301 gcattggata aaacaatttt ttcaaactat cctgatgttg aaacattcct cactcaagag
      361 ataaaagagc taatcggaga aagaacaaaa gatttttatg agtatattga taaacaattt
      421 tatgaaagct ataaaaatac taccgtacct cccgaaatag ttgtaaagca ccgtgaagac
      481 ttttatagct cgtttctatt tctccctgct agattacaaa aatttatagc tttatggagc
      541 atactaacag aaactgaaca agaaaatata ggtaaaacta ttgaattatt agctatgaga
      601 gggagataaa aatcccccca tattcgccaa tagcaaacct tagcactaat atttttcttt
      661 tagggtagcc gaaagtgccg aaaatgcgac ctatagcata ccacgcgcca caacgttcaa
      721 caatcatgaa gcgcgtgttt tctcagggtt cactcttgat tcgttattag attaaatatg
      781 taagcattag ttagccctta tccaataaaa accaacattt aataaaaaga tataactcat
      841 taactttttt aatttttatc tttcaaacaa aac
//
LOCUS       DAVMHG010000254          864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVMHG010000254 DAVMHG010000000
VERSION     DAVMHG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.258"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(7..858)
                     /locus_tag="VYR08_002123"
     CDS             complement(7..858)
                     /locus_tag="VYR08_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467572.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
BASE COUNT          265 a          128 c          143 g          328 t
ORIGIN      
        1 tacaaattat aggtttgaaa aatcattaat ttctttttcg agtattttaa ctaatgcctc
       61 ttctttggtt agtcgtaata aagttattcc tttatcaatc aaatcggagt tatgttgtag
      121 tattcccaag cgtacttgag agaatccaag aatatcgtat atttttaatt cttctgctaa
      181 taccaaaagt tgtagggtga ttgtttcaca ttctttgaat aaatgatgtt gaaaatatac
      241 agtggagaga ttggctaaaa aagatgataa aaatgctttt gtttctcgga agtatttata
      301 tttctcgatg gttttcataa ttctatcaat aagaagggga agattttcag aaggaaagaa
      361 aaagagaatg gttccaagca atttcaaatc actaatgtac catgtatcca taggttcaag
      421 atagtcccaa atttcttcat acaaatcttt caagacgtgg ttgtttttga atcctttagt
      481 tcgtaactct gtgacaattt ttgtatgacg atagatattt tgaataggaa catcgtgatg
      541 tgtcttaaga tatttttgac atttctcagt gagttcaacc atttttctag tatcttgaaa
      601 agtagacgga ttttggctct ctacaataat atctcgcctt ttactaggat ggtattcgtt
      661 gcatatatac ttgaattctg ccaagctcat atcaatttgt tcaagaagaa ataccatgtt
      721 ttcaaaactg ggaataactt taccgtgttc aatatttgtt agagttgttc gatgtatgac
      781 ttgtccacat acatcttgtt gggtcaatcc ttttgacttt cgaatttctt taaaaatttg
      841 tccataatcc catctcatat tgtt
//
LOCUS       DAVMHG010000255          862 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVMHG010000255 DAVMHG010000000
VERSION     DAVMHG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.259"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            275..750
                     /locus_tag="VYR08_002124"
                     /pseudo
     CDS             275..750
                     /locus_tag="VYR08_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT          321 a          142 c          119 g          280 t
ORIGIN      
        1 atttgacaag ttatcctaaa agtattatta tgttgttgtg ttatagattg attgaatcta
       61 actaaaggat cctattcaat tactagaact atcacatact caaggtcagc tcacagatga
      121 gcaactattt tggttacaat gtctactaaa tttaagtcaa acaaataatt tagtcaaaat
      181 taaaaaaata gaggaacata aatatgatta caaaacagaa tgtaatagtg ttctacgatt
      241 tttactagat aaaactgtaa attctgaagg aaggatcact tcttcaacag aatttggaaa
      301 tttcgtaagt aatttatcat tccaacacgg aatagctgga ctactgtttc ctctaaataa
      361 attgtacccc ccagaactgg attctaaaat actctctatc atcaagaagg cagtgacaat
      421 tagaacgaca cacacatata aatatcaata ctcactgcta tttggtgatg caggctatct
      481 atggttactc ctacatttat tttctatcag taaaaatcaa tactatctac aattagcaaa
      541 cgtctccgct aagaaattaa tagagaatta tgatactcta gaggaaatag actttgcatt
      601 gggaaaatct ggtgtcctat tatcattaat aaaatactat caatttacca atgacaatac
      661 tcttaaaatt ttcatccaca atagtatagg ggaaatttag cattatttcc tacaaagaga
      721 tatagccaaa gaaagcattt tagactatag ctttgctcat ggatattgtg gaattgcata
      781 ggctttattt gcctattcta aagtcttaga accttctatg ttctataatg atctccatac
      841 attccatact gaattaaaaa aa
//
LOCUS       DAVMHG010000256          848 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVMHG010000256 DAVMHG010000000
VERSION     DAVMHG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.260"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            83..784
                     /locus_tag="VYR08_002125"
     CDS             83..784
                     /locus_tag="VYR08_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV3467573.1"
                     /translation="MFAGAFLTFSTAAGAVGADLINKIAPGSGRFLFPFVFAWGLAYI
                     VFLNAELVTSNMMFLTAGSFLKKISWRKTAEILLYCTLFNLIGALIAGWGFAHSAAYA
                     NLTHDSFISGVVEMKLGRSNELVLLEAILANIFVNIAILSFILVKDGGAKLWLVLSAI
                     YMFVFLTNEHIAANFASFAIVKFSVAADSIANFGVGNMLRHWGVTFIGNFIGGGLLMG
                     LPYAFLNKNEDTYVD"
BASE COUNT          212 a          148 c          190 g          298 t
ORIGIN      
        1 atttatctca aagattgaat ttgcttgcaa taagaaagaa agtctttata gtcaaagcaa
       61 atttaagtat gcgattcgtt cgatgttcgc aggtgcattt ttaaccttca gtactgctgc
      121 aggtgcagtt ggggctgact tgattaataa aattgcacca ggtagtggac gcttcctctt
      181 tccattcgtt tttgcttggg gcttggccta cattgttttt ttgaatgccg agttggtcac
      241 ttcaaacatg atgttcttga ctgctggtag tttcttaaaa aaaatctctt ggagaaaaac
      301 agctgagatt ttactatact gtaccttgtt caaccttatc ggagccttga tagcagggtg
      361 gggctttgct cattcggcag cctatgcgaa tctgacacac gatagtttca tctcaggtgt
      421 tgttgagatg aagttaggcc gctcaaatga attggtcttg cttgaggcga ttttggcaaa
      481 tatttttgta aatattgcga ttctgtcatt tattttggtc aaagatggtg gtgccaaact
      541 ttggcttgtg ttgtcagcta tttacatgtt tgtattctta acaaacgagc acattgcggc
      601 gaactttgct tctttcgcga ttgtgaaatt cagtgttgct gcggattcaa ttgccaactt
      661 cggtgttgga aatatgcttc gccactgggg tgtgactttc atcggaaact ttatcggagg
      721 aggcctcttg atgggtcttc catatgcctt cctcaataaa aacgaagata cttatgtaga
      781 ttaagaaaat gagcacgatt gagtcgtgct tttttcattt tcaaaataag gtaatagcta
      841 tttcttat
//
LOCUS       DAVMHG010000257          830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVMHG010000257 DAVMHG010000000
VERSION     DAVMHG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.261"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            275..>830
                     /locus_tag="VYR08_002126"
     CDS             275..>830
                     /locus_tag="VYR08_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV3467574.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNH"
BASE COUNT          237 a          147 c          191 g          255 t
ORIGIN      
        1 tttccattat aggagcaaca gagtgggagt agtcatctaa ggactaattt atgtattttt
       61 acgagtcagt atctttggat actggttttt acttttctag actttttgac tacttgttaa
      121 aactgggata attttcgact gtttaacagt tattatgcaa agtctaaaag attagaattg
      181 tcaaaacaat ccgtctaggc ttgattttat cctttattta ctataaaatg agaaggaaaa
      241 atgtcaaact tttatattgc aaataggaga aatcatgaca aaaacattaa aacgtcctga
      301 ggttttatca cctgcaggga ctttagagaa gctaaaggta gctgttcagt atggagcaga
      361 tgctgtcttt atcggtggtc aggcctatgg tcttcgtagc cgtgcgggaa actttacttt
      421 cgaacagatg gaagaaggcg tgcagtttgc gaccaagtat ggtgccaagg tctatgtagc
      481 ggctaatatg gttatgcacg aaggaaatga agctggtgct ggtgagtggt tccgtaaact
      541 gcgtgatatc gggattgcag cagttatcgt atctgaccca gccttgatta tgattgcagc
      601 gactgaagca ccaggccttg aaatccacct ttctacccaa gccagtgcca ctaactatga
      661 aacccttgag ttctggaaag agctaggctt gactcgtgtc gttttagcgc gtgaggtttc
      721 aatggaagaa ttagctgaaa tccgcaaacg tacagatgtt gaaattgaag cctttgtcca
      781 tggagctatg tgtatttcat actctggacg ttgtactctt tcaaaccaca
//
LOCUS       DAVMHG010000258          810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVMHG010000258 DAVMHG010000000
VERSION     DAVMHG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.262"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>810)
                     /locus_tag="VYR08_002127"
     CDS             complement(<1..>810)
                     /locus_tag="VYR08_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV3467575.1"
                     /translation="EALSRTKNELAGVKSAQATYKETTTRRLSELTNLANGKANKSEL
                     TQTAEELASKIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVSDSPA
                     TGFDKAIRLTSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNS
                     GISPIFTLKDENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLAT
                     SSKEAPEDIEGQISTVESTFKQRADSLAAGVNRLTEGLRTKADISALNVTA"
BASE COUNT          184 a          216 c          163 g          247 t
ORIGIN      
        1 tcagcagtca cgttgagtgc gctgatatcg gctttagttc taaggccttc agtcagacga
       61 ttcacaccag cggcgagtga atcagcacgc tgtttgaagg tcgattcaac tgttgaaatc
      121 tgaccttcta tatcttcagg agcttctttt gagcttgttg ccaaacttcc gtcttccagc
      181 tggggcgcaa gaacatctaa atattctccg acctcagcat ttacgagata cacatagcca
      241 attgatgcga ctccagccct atttctagcg ctagtaaacg atagctttgt ccaattttca
      301 tcctttaatg taaagatggg cgaaataccc gaattatcat tgacttgcca ataagtttgt
      361 agcttgacct tttgacctct tctgcctttg acccaacaag acatcgtgta tgtgccttgc
      421 gagatataaa atccatcttg agcaatacct atttggtctc tagcattgct tgaggtcaat
      481 cgtatcgctt tatcaaagcc agttgctgga ctgtctgata catcaatcgt ctttgccgtc
      541 ccagcgcctg acggtctaaa agtacctgat gcccacaatc ctctggctag catcatgcgc
      601 tttgtacccc ggatataatt cctacccccg acctgcacac tcgctatctt actagctagc
      661 tcctcagctg tctgcgtgag ttctgacttg ttggctttac cattggccaa gttggtcagt
      721 tctgacagtc tacgagtcgt cgtctcctta tacgtcgctt gcgctgactt cacgccagcc
      781 agttcattct tagtccggct aagtgcttca
//
LOCUS       DAVMHG010000259          805 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVMHG010000259 DAVMHG010000000
VERSION     DAVMHG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.263"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..784)
                     /locus_tag="VYR08_002128"
     CDS             complement(<1..784)
                     /locus_tag="VYR08_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467576.1"
                     /translation="MKNVSKHYGHLIILKDINFALNKGEIVGQVGRNGVGKSTLMKIL
                     VQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQE
                     LDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKN
                     LALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDR
                     DIFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVV"
BASE COUNT          271 a          151 c           88 g          295 t
ORIGIN      
        1 caactacttt aatagagttt tcgttaaaaa atttaaaaag ctcactactt tgaatattcc
       61 cagacatagt aattctcagt ccctcttcct gaacaatatc cccaaattct tgtttggtaa
      121 tgaaaatgtc tctatctgta gctgatgaaa aagctatctc aaacaagaaa ttatgactat
      181 cttttcctac tttttgaaat gtcaaaagcc cgttctccaa gaaaagaacc ctctcacaaa
      241 tttcttcaat atcttctaat ttatgactcg atattaaaat tcccacattt tcatgtaaag
      301 ctaaattttt tagaaccgat aaaactattt gtgatgattc aatatctaaa ccattagtcg
      361 gttcatctaa aatcaatata tcaggttccg taacgagagt tagaagcaaa gctaattttt
      421 gttttgtacc caaagaatag gtctttactt ttttattaat agactgagtc aaatctaact
      481 cttggatcaa acttctaaat ctttcttgat tgtagtcaac accatataaa tttgacaaat
      541 attttaaatt ctctaaacct gttttagata aaaataattt tggttcttcg attaaatacc
      601 caacattatc actgcttata atattacctg aagtcggttg attattctga acaagaattt
      661 tcatcaacgt actcttacca actccatttc tccctacttg accaacaatt tcacccttgt
      721 taagtgcaaa atttatgtct ttgagaatga ttaaatgacc ataatgctta cttacatttt
      781 ttattactcc attaatatta ttggt
//
LOCUS       DAVMHG010000260          790 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVMHG010000260 DAVMHG010000000
VERSION     DAVMHG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.264"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..297)
                     /locus_tag="VYR08_002129"
                     /pseudo
     CDS             complement(1..297)
                     /locus_tag="VYR08_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(284..535)
                     /locus_tag="VYR08_002130"
                     /pseudo
     CDS             complement(284..535)
                     /locus_tag="VYR08_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(535..705)
                     /locus_tag="VYR08_002131"
     CDS             complement(535..705)
                     /locus_tag="VYR08_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467577.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
BASE COUNT          257 a          166 c          114 g          253 t
ORIGIN      
        1 gattaaggaa tctaataaat ttgcgaagtt aatcggtttc ttgtcttcat cataagcttt
       61 tacagttact tgggttgtaa gtattccctc ttttccctcg gctcgatagc cttgtccata
      121 taaaacaaaa acgagatttt gatgatcatc tacaaaggca tcaaccccat tctttatgtc
      181 ttgactttca aggaattcca taacgttttg aagataggat tcgtaaaata gtgggtagtt
      241 gtgtttttta tggtaatcat ctaaaaatgt tacttcaaac tcacatggag agtaattttg
      301 actttgaaca gcctaaaagt gccatcaaat ttgaattgga ataaatcaaa taaatagccc
      361 catcctcatc aatccaacct ttgctcaaag acaactccaa ccgatctttt aaaactgagt
      421 aaaccacctt aacctccagt ttcatattct tataccgttc actctcaaat aaaagtttgg
      481 ggagcttata ataacgctct gatgtctgat attgattagc ggtaatacgc ttcattattg
      541 tccctccaag actaaaattc caacatttcc aaattcatca aatcggatta aacctacttg
      601 ttccatttca tcaactaact gagttgcttt tacaatatca actcccatga tagtcatgag
      661 atgactcttc acgaattgac gtgatgactg tccttccttt tgcataatta cctccgaaac
      721 acaaaaaaag gggtagacaa tctagtgtct acccccgaaa gtttattaaa acaaaaatcc
      781 tgccaaagaa
//
LOCUS       DAVMHG010000261          789 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVMHG010000261 DAVMHG010000000
VERSION     DAVMHG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.265"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..789)
                     /locus_tag="VYR08_002132"
                     /pseudo
     CDS             complement(1..789)
                     /locus_tag="VYR08_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          244 a          145 c          175 g          225 t
ORIGIN      
        1 aatcttttct ttatctttga ggaaggtttt aaagacagtc tgaaaaatag gatgagcctg
       61 cttaagattg tcctcaataa gtccgaaaaa tttctctggt tccttattct ggaagtgaaa
      121 cagcaagagc tgatagagct gatagtggtg tttcaagtct tctgaatagc tcaaaagctt
      181 gtctaaaatc tctttattgg ttaaatgcat acgaaaagta ggacgataaa atcgcttatc
      241 actcagttta cggctatcct gttgtatgag cttccagtag cgcttgatag ccttgtattc
      301 atgggatttt cgatgaaact gattcatgat ttgaacacgc acacgactca tagcacggct
      361 aagatgttgc acaatgtgaa agcgatcaag aacgatttta gcattcggga gtgaaacagt
      421 ctgggagact gtttcagcct gagcctagaa atttgaaagc gaagctgttt agccaagtca
      481 tagtaagggc taaacatatc catagtaata attttgacgc gacatcggac agctctatcg
      541 tagcgaagaa agtgatttcg aatgatagct tgtgttcttc cctcaagaac agtgatgata
      601 ttgagattgt taaaatcttg cgcaatgaag ctcatctcca tctcccgatt gaaacagtca
      661 ctccccggac tgtttcaacg tcccaggaca taatctcagg aagacgcgaa aaatcatgct
      721 caaagtgaga atcattgagc ttgcgaatga cagttgaagt tgagatggaa agctgatggg
      781 caatatcag
//
LOCUS       DAVMHG010000262          787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVMHG010000262 DAVMHG010000000
VERSION     DAVMHG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.266"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(43..189)
                     /locus_tag="VYR08_002133"
     CDS             complement(43..189)
                     /locus_tag="VYR08_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467578.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            347..565
                     /locus_tag="VYR08_002134"
     CDS             347..565
                     /locus_tag="VYR08_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV3467579.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          251 a          116 c          146 g          274 t
ORIGIN      
        1 cgaaatctag tggaatgaat ctggaatagt ccatcgagct ttctaatact ctttgaaaat
       61 ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
      181 ggttttcatt gagtataaca cattgttaga agttggttta aatttcctaa tcagtttgtt
      241 cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag
      301 ccatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga aaaaaatatc
      361 tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata
      421 cgtaaatggt actgctattc tttatcctta tttacgttct attgtttcaa tagtttcggc
      481 aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa
      541 atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt
      601 aaaagcagta agaaaaatgt cttgcattat acagcaactt tttgggaatg agtagatgga
      661 ttgaataaaa tttgattaag agtggatgat ttatctgtag attattattg gacagttagt
      721 cttgaagtag tctaagaatt aggttataat cagtagaagc cttgctaata atgaggaggt
      781 tagttta
//
LOCUS       DAVMHG010000263          776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVMHG010000263 DAVMHG010000000
VERSION     DAVMHG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.267"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..518
                     /locus_tag="VYR08_002135"
     CDS             <1..518
                     /locus_tag="VYR08_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467580.1"
                     /translation="DIFSINTGLSYKKGDLSINKGVRIIRGGNIKPLEFSLLDNDYYI
                     DTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSDI
                     ISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVE
                     KLFEKVNQLWK"
     gene            complement(466..>776)
                     /locus_tag="VYR08_002136"
     CDS             complement(466..>776)
                     /locus_tag="VYR08_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV3467581.1"
                     /translation="TLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLM
                     IFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE
                     "
BASE COUNT          271 a          114 c          116 g          275 t
ORIGIN      
        1 aagatatttt ttcaataaat acaggtcttt cttacaagaa gggcgattta agcattaata
       61 aaggtgttag aattatacgt ggtggtaata ttaagccttt agaattttct ctgttggata
      121 atgattacta cattgacaca caattcatct cctctgagca agtttattta aaacataatc
      181 agctaataac acctgtatca acctctttag aacatattgg aaagtttgca agaatcgata
      241 aagactatga tggtgttgtg gctggtggat ttattttcca attaacacca ttcgaaagtt
      301 cagatattat ttcaaaattt ctattattta acttgtcctc tccgttattt tataaacaat
      361 tgaaagcaat aactaaacta tcaggtcaag ctttatataa tattcctaaa actacactaa
      421 gcgagctatt aattccgtta gctccttttg aggaacagga acttattact caaaaagttg
      481 agaaactttt tgaaaaagta aatcaacttt ggaaatgatt cttttcatct cttcatgatt
      541 agaaataggg attaataatt cggagatact ggtactattt aatgttttcc cttttatagc
      601 atcttttgaa tcacctaaag tagagataag tggcaaaaat atcattaagt aatctctgat
      661 aatattttct ttattagcat aggggaatat cgatataatg gcttcattat gagtggcagg
      721 aatatccaat atggcaactt ttccaataga taatttaaaa ctcattaata aagttc
//
LOCUS       DAVMHG010000264          764 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVMHG010000264 DAVMHG010000000
VERSION     DAVMHG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.268"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            168..354
                     /locus_tag="VYR08_002137"
                     /pseudo
     CDS             168..354
                     /locus_tag="VYR08_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT          282 a          109 c          138 g          235 t
ORIGIN      
        1 aagaaaataa acctactttt gatgtatcga aaaagaaaga taacccacaa gtaaaccata
       61 gtcaattaaa tgaaagtcac agaaaagagg atttacaaag agaagatcat tcacaaaaat
      121 ctgattcaac taaggatgtt acagctacag ttcttgataa aaacaatatc agtagtaaat
      181 caactactaa caatcctaat aagttgccaa aaactggaac agcaagcgga gcccagacac
      241 tattagctgc cggaataatg tttatagtag gaatttttct tggattgaag aaaaaaaatc
      301 aagattaaga caaaagctat agaaaaaaat ggtttatgta ctgagattag atagtgaggt
      361 gatgacatag ttttgtgaaa acggccattt ataactcaat tatttagttt acttcacttc
      421 actattgata ctatttggag ttattaatgg acttagttta gataactaat gaattgattg
      481 aaagggttag tattgacaat attggtcata ttgattagaa aatagagtct atcaaaattt
      541 aaaggctaat agaggtgatg agacaatttc aaggaattaa gtagcgtttg gttgcttaat
      601 tcctttttat ttggaaggaa gtttttcgta accgcccaat aacgaagtct attggacggt
      661 tacgatatga tcactgactt cgtcagtctt atctacaacc tcaaaactgt gttttgagca
      721 acctgcggct agcttcctag tttactcttt gattttcatt gagt
//
LOCUS       DAVMHG010000265          761 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVMHG010000265 DAVMHG010000000
VERSION     DAVMHG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.269"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(269..>761)
                     /locus_tag="VYR08_002138"
     CDS             complement(269..>761)
                     /locus_tag="VYR08_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV3467582.1"
                     /translation="DILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIV
                     EEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPD
                     KYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFN
                     GRK"
BASE COUNT          228 a          142 c          135 g          256 t
ORIGIN      
        1 attttttcgt aaaaaaatac cccctacatt ttcgtaggaa gtatttttag tcaaccatac
       61 cagttgacag atatcccact taggacattt tcctacaagg ggtccctagc gcttagtggg
      121 aatttgtacc ccttatcgat acaaattccc cgtaggcgct agggacctct ttagctcctt
      181 ggaagctgtc agtagtatac ctaataattt atctacattc cctttagtaa cgtgtaactt
      241 tccaaattta caaaagcgac tcatagaatt atttcctccc gttaaataat agataactat
      301 taaaaataga caatacttgc tcataagtaa cggtacttaa attgtttact ttggcgtgtt
      361 tcattgcttg atgaaactga tttttagtaa acagttgacg atattctcga ttgacccatt
      421 ttgaaacaaa gtacgtatat agcttccaat atttatctgg aacatctgtg gtatggcggg
      481 taagttttat taagacactg tttacttttg gtttaggatg aaagcattcc gctggcagct
      541 taagcaattg ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt cggtgaatat
      601 ccaaggtacg cttgtagaat ccttcttcaa caatcagata gatgtcagac gcatggcttt
      661 caaaaaccac ttttttaata atttgtgtgc ttaaatggta aggaatattc ccaacaattt
      721 tatacctctg tttgttaggg aattgaaact gtagaatatc t
//
LOCUS       DAVMHG010000266          735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVMHG010000266 DAVMHG010000000
VERSION     DAVMHG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.270"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            40..270
                     /gene="blpU"
                     /locus_tag="VYR08_002139"
     CDS             40..270
                     /gene="blpU"
                     /locus_tag="VYR08_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV3467583.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          254 a           83 c          171 g          227 t
ORIGIN      
        1 aacaagacaa atattaaaaa taaaaaagag gtattcgtta tgaatacaaa aaagatgtca
       61 caatttgaaa ttatggatac tgagatgctt gcttgcgttg aaggtggcgg atgcaattgg
      121 ggagattttg ccaaagcagg tgttggagga ggagcagtac gaggtcttca gctaggaatt
      181 aaaacaagaa catggcaagg tgcagcaact ggtgctgtgg gaggagctat acttggaggt
      241 gtggcctatg cagcgacatg ttggtggtaa ttatggattt taaaagtttt attattggtt
      301 tagtagttgg tatatttggt ccttatatgg atgatttaat tagaaaaaaa tttttaaagt
      361 cttcggagaa gaaaacagaa aaatctgtta aaaaataatc aaaactataa atgatgaatc
      421 tgaatcaaaa ttattttgcg catgtaaata ggagtcttat agtaacgagt caaaaaagga
      481 gtaactatga atcgtaattt agaacggtgt tatctattct gactaggaat agatcatacc
      541 agaggtggct tagaaatagc ggggacatta gaaattgaag gaactttaaa taggatgtcg
      601 taaatgttgc tatcaatgat ttatttgtcc caagcttgcc tagggtgtca gtaaaaaatc
      661 attttccttt catatcatat ttttagtagg taggatgttt gttctgtcta ttttttgcca
      721 aaaaaagtgc agtcg
//
LOCUS       DAVMHG010000267          728 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVMHG010000267 DAVMHG010000000
VERSION     DAVMHG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.271"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(42..>728)
                     /locus_tag="VYR08_002140"
     CDS             complement(42..>728)
                     /locus_tag="VYR08_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV3467584.1"
                     /translation="ALINSVRWAVIGVIIQVPLAVSVAITLSKKLKGWKISRNLYIVP
                     SIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFKDSVLLTPGLNIVAMTGAYIFFAG
                     ASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYITIPMIKGTIKTVSIMAATSGFLL
                     YNEVFFLTNGAAGTKSISFVIRELAVASSRTQYARANTIGVIQILGGMLIIVCINILF
                     RERKRLKGGK"
BASE COUNT          254 a          141 c          101 g          232 t
ORIGIN      
        1 tttgcctttt ttactaatac catttatatg tgtattcata atcatttccc acctttcagt
       61 ctttttcttt ctctgaataa aatattaata caaacgataa tcaacattcc acctaagatt
      121 tgtataactc caattgtatt tgcacgagca tactgagttc gtgagctagc cactgctaat
      181 tctcgaataa caaaactgat actttttgtt cctgcagcac catttgtcaa aaagaatact
      241 tcgttataga gcaaaaatcc tgaagttgct gccataattg aaacagtttt aattgtcccc
      301 ttaatcatcg gaatcgtaat ataccactct ttcctccaac cagtaatatt gtctaaaata
      361 gcagcttctt gaacttcttc tggaatagca aaaatttgcc caagaatcat aatggttgat
      421 gctcctgcaa agaagatata agcgccagtc atagctacta tatttaatcc tggagtcaac
      481 agtactgaat ctttaaacga tggattaaat agatgaataa tttggttaac aacaccatag
      541 tttggattat agatttgaag aaaaatcaag cccatagcag cactagaaat gatacttggt
      601 acaatataaa gattcctaga tatcttccag ccttttaatt tcttagataa tgtaattgca
      661 acagatacag ctaaaggaac ttgaataata acaccaatta cagcccaacg aacagaatta
      721 attagagc
//
LOCUS       DAVMHG010000268          695 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVMHG010000268 DAVMHG010000000
VERSION     DAVMHG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.272"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..482
                     /gene="rlmH"
                     /locus_tag="VYR08_002141"
     CDS             3..482
                     /gene="rlmH"
                     /locus_tag="VYR08_002141"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV3467585.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            526..599
                     /locus_tag="VYR08_002142"
     tRNA            526..599
                     /locus_tag="VYR08_002142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Arg,seq:tct)
BASE COUNT          239 a          102 c          136 g          218 t
ORIGIN      
        1 tcatgaaaat taaagttgta acagttggga aactgaaaga aaagtattta aaagatggta
       61 tcgcagagta ttcaaaacga atttctagat ttgctaagtt tgaaatgatt gagttatcag
      121 atgaaaaaac accagataag gccagtgaat cagaaaatca aaagatttta gaaatagaag
      181 gtcagagaat tttatcaaaa attgctgacc gtgatttcgt tattgtgtta gccattgaag
      241 ggaaaacttt cttctcagaa gaatttagta agcagttaga agaaacttct ataaaaggat
      301 tttctactct tacttttatt attgggggaa gtttaggatt gtcatcatct gtaaaaaata
      361 gagccaatct ttctgtcagt tttggtcgcc taaccttacc ccatcagtta atgagactag
      421 ttcttgttga acaaatctat cgcgctttta cgattcagca gggattcccc taccataaat
      481 agaaaattga cttttaattg aatttttggt agaataattg tattaggtct catagctcag
      541 ctggatagag cattcgcctt ctaagcgaac ggtcgcaggt tcgaatcctg ctgggatcaa
      601 tataatagca aagctgggaa ttttcccggc ttttttctta aaaaagtaca ctttgggaga
      661 aaaaaatgac agttgagaga attttatcta aaacg
//
LOCUS       DAVMHG010000269          689 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVMHG010000269 DAVMHG010000000
VERSION     DAVMHG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.273"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            42..449
                     /locus_tag="VYR08_002143"
     CDS             42..449
                     /locus_tag="VYR08_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV3467586.1"
                     /translation="MWKVAQRELVTGFYYGTPSENEQLFGARRKIPEYKFVAEVVSYD
                     DATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIK
                     VPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          224 a          115 c          145 g          205 t
ORIGIN      
        1 ctgaaaagtt tgaagctatc aaacaagact tggtggacga gatgtggaag gttgcccaac
       61 gtgaactggt tacaggattt tactatggta caccatctga aaatgagcag ttgtttggtg
      121 ctcgtcgtaa aattcctgag tacaagtttg tcgctgaagt ggtttcttat gatgatgcga
      181 cacaaacagc aaccattcgt caacggaacg tcattaacga aggggaccaa gttgagtttt
      241 atggtccagg tttccgtcat tttgaaacct atattgaaga tttgcatgat gccaaaggaa
      301 ataaaatcga ccgcgctcca aatccaatgg aactattgac tattaaagtc ccacaacctg
      361 ttcaagctgg agacatggtt cgagctctta aagaggggct tatcaatctt tataaggaag
      421 atggaaccag cgtcacagtt cgtgcttaat gtagttgttt agttttaaaa aattatgcaa
      481 agctccatat acaacactta aacgagatta aagaatggcg aaatcccctg atgcgcaaga
      541 gattagctgt cttttttatt ttttaagtga taaagtcgga gcttaggtat caaagcctat
      601 caaattaaac aaagaagcga tgtcttagat attttgaaaa aaattaataa gcagaaaact
      661 ctctattatt ttgttgtata gagtttttt
//
LOCUS       DAVMHG010000270          684 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVMHG010000270 DAVMHG010000000
VERSION     DAVMHG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.274"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..671
                     /locus_tag="VYR08_002144"
     CDS             <1..671
                     /locus_tag="VYR08_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV3467587.1"
                     /translation="ILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRILKLMA
                     CFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEV
                     LELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSKENGITGVTQKTINA
                     DEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKIFN
                     EFLF"
BASE COUNT          263 a           68 c          131 g          222 t
ORIGIN      
        1 taattttaag taatgaagat ttaggtgcaa ctaatgccag aaacataggt atcaaaaatt
       61 ctagaggtaa gtatatatca tttttagacg atgatgatga atatatgcca gatcgaattt
      121 taaagttgat ggcttgtttt aaaaagagta gaatgaagaa tttagcttta gtttatagtt
      181 atggaataat aatttatcca aatggtacac gagaagagga gaagaccgat tttgttggaa
      241 atcccttgtt tgttcaaatg gttcacaata tagcaggtac gtcattttgg ttgtgtaaaa
      301 aagaggtgct agaattaatt aatggttttg agaaaataga ttcacatcag gacggtgttg
      361 ttttattaaa actacttgtt caaggatacc aaattgatat agtgcgagaa ttcttggtga
      421 attactacgc tcacagtaaa gaaaacggta tcactggagt gacacaaaaa acaattaatg
      481 cagatgaaga atattataat tactgtagga aatattttaa tttattgagt ttcaacgaga
      541 gaatattggt tacaaagaaa tattattctt taaacataaa gcggttacta ttaataggag
      601 acaaatgcaa ggctttaaaa gtaatcaaga aggcaagaga agaaaaaatt tttaacgaat
      661 ttctttttta aaatatatgt tatt
//
LOCUS       DAVMHG010000271          675 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVMHG010000271 DAVMHG010000000
VERSION     DAVMHG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.275"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..257)
                     /locus_tag="VYR08_002145"
     CDS             complement(<1..257)
                     /locus_tag="VYR08_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467588.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLC"
     gene            complement(254..601)
                     /locus_tag="VYR08_002146"
     CDS             complement(254..601)
                     /locus_tag="VYR08_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEV3467589.1"
                     /translation="MTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIG
                     IVATIKGILPHALIEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYL
                     LLILAALIEGIKL"
BASE COUNT          247 a          119 c           82 g          227 t
ORIGIN      
        1 tcacacaaca actgcaaatt ttcttctata gtaaatggaa caaccccaag ctgattggta
       61 tcaaggtata gtacatcttt ggagacacaa ttctttaaac cactatcaaa agtctctagc
      121 ccagctaaaa gttgtagaag ggtgctcttt cctgaaccat tctctccttc aactctgtaa
      181 attcccttat caatctctag tgatatattt ttcaatatac atttatcata acttttagat
      241 tcattttcaa ttttcataat ttaattccct ctatcaacgc tgctaaaatt aataaaagat
      301 aagaaatgat ataccatttt aaattacttt ttatataaat aagtatattt ttaattcccc
      361 tatccttcaa taatcggtat aaaagatact gactcatacc ttgataccaa ataaaattcg
      421 gtatttcaat aagagcatga ggtaatattc cttttattgt agctactatt ccaatcatct
      481 gaccagatat acccaattgt attcctaaag atagcaaatc aaaaagttgc aataagggcg
      541 aaatcggaaa acagaataga tatagcaaaa aatttgtaga atttgcctgt aaaactgtca
      601 atgtaaattg taattccagt ttcggaggta tagtagcaaa agtctcattt tttaatataa
      661 agcttaaaag taaac
//
LOCUS       DAVMHG010000272          657 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVMHG010000272 DAVMHG010000000
VERSION     DAVMHG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.276"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..583)
                     /locus_tag="VYR08_002147"
     CDS             complement(<1..583)
                     /locus_tag="VYR08_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467590.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVK"
BASE COUNT          212 a          140 c          105 g          200 t
ORIGIN      
        1 tttttacata cccagtatag tttgagtttc tatagtattc agtgataaac ttccattttc
       61 tttgagcaac atggatataa gtacttgtta tgtagtatgg atatgggctt tgtgaatcca
      121 agtaagactg ataagcttgt ataccaaaat atgctccacc aattattgca ccccatggac
      181 cccccaataa agcacctatc ctaccaatca tataactgat tccagcacca gtcatgaagt
      241 tagcgaatgt gttagcttgt ttattcccat gtattgtgtt gacgtaattc caaacattag
      301 gatcgtatga tctaaaagat atatttaggt cgatttcatt cttttgataa gccatataaa
      361 atgccccatt gatatagacg ccgtcagcac gtcgttcaat agtgtctaca cttccatctg
      421 gattgacaac ctcaagaact tcatcgctta aaatatttac ttgcgtatct ccgaaccgca
      481 ctgatgagcc attctcaaac tgagcctcac cagatacaac tttagagttt gccgataagc
      541 tatcatcagc aaaaacaaca agcgacgggg aaatgctaga catacagaaa acagacataa
      601 ctagcaaaca catgcattta aacatcttag acataacgga aactcttttg tattttt
//
LOCUS       DAVMHG010000273          656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVMHG010000273 DAVMHG010000000
VERSION     DAVMHG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.277"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            244..>656
                     /locus_tag="VYR08_002148"
     CDS             244..>656
                     /locus_tag="VYR08_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467591.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYIEDLKLKCNIA"
BASE COUNT          306 a           79 c           96 g          175 t
ORIGIN      
        1 gaatgaaaaa taggatatag agaataacag caatattaaa aagatcagga ctgataataa
       61 acaaaaagaa agtacaacga ctagttcaaa agctaaaact tcaagtaaaa agttattcaa
      121 gaaaatctag aaaatactca tcctacaaag gacaggtagg aaaaatatca gacaacaaaa
      181 taaaaagaaa tatttattac cgaaaaaaag aggaaatcga gtcaagtacc aacaggctca
      241 tctattccaa aaagatattt actcgatgga aaaactccta gaattagtgc tacaaatatt
      301 gacaatggta tcttaggtta ctacgaagat atagatgata aaaattatag agtttttgaa
      361 aattttatat ctgtttcatt tttaggagca gtcttttatc ataaatataa agcaagccta
      421 gatatgaaaa ttcattgttt aaaattaaaa aataaagaat tgaataaaga agtagcattc
      481 tatttaactt caattattag gcaagcttta aaaaatactg aatataaaga tcaaatatct
      541 agcacagtat tgccagatat caaaattaaa ttaccgatag acagtagagg tactccagat
      601 tggaattaca tggaaagata tatagaagac ttaaaattaa aatgcaatat agcaaa
//
LOCUS       DAVMHG010000274          652 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVMHG010000274 DAVMHG010000000
VERSION     DAVMHG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.278"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..584
                     /locus_tag="VYR08_002149"
     CDS             105..584
                     /locus_tag="VYR08_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3467592.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          246 a           82 c          150 g          174 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa aggctgtata at
//
LOCUS       DAVMHG010000275          650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVMHG010000275 DAVMHG010000000
VERSION     DAVMHG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.279"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..534
                     /locus_tag="VYR08_002150"
     CDS             <1..534
                     /locus_tag="VYR08_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV3467593.1"
                     /translation="VGVQDFKKEFCVSNNKKIICYVGRLDPEKRPDRFLEFAEKLFLV
                     REDVIFIMAGNGSMWAALKEKICHLKYRDNFRLLGEIYPATIVYQISDLLYIPSDTEG
                     IPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYL
                     KYIGENSKSKIRKHESI"
     gene            558..>650
                     /locus_tag="VYR08_002151"
     CDS             558..>650
                     /locus_tag="VYR08_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV3467594.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCL"
BASE COUNT          228 a           59 c          144 g          219 t
ORIGIN      
        1 gtaggtgttc aagactttaa aaaagaattt tgtgtttcaa ataacaaaaa aatcatttgt
       61 tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt tgcagaaaaa
      121 ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag tatgtgggct
      181 gctttgaaag aaaaaatttg ccatttgaaa tatagagata atttcaggtt acttggtgaa
      241 atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc ttctgataca
      301 gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt tttagctagt
      361 aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt atttgagaag
      421 gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc tgagtatttg
      481 aaatatatag gtgagaatag taaatcaaaa ataagaaaac atgagagtat atgatgaatt
      541 attagagaag agtagtcatg gataggaata tagatcaaga attagtaagt attataatac
      601 caacacataa taggtatgaa tctctgatta gagctgtgaa aagttgttta
//
LOCUS       DAVMHG010000276          646 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVMHG010000276 DAVMHG010000000
VERSION     DAVMHG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.280"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          274 a           95 c           77 g          200 t
ORIGIN      
        1 cagaaaactc catctcaata aactaactgt acctttaatt aaaaatagag ctttcttata
       61 atagacatag gctataaaat aattgaatgg aatcaataac aaattcaaca aaatactttt
      121 tatatcatta ctataacttg tcaccggaat caaacataac aacaatgaaa attgtaagaa
      181 agagattaaa tgcgtcaaca tcattagttg tgcattctta aaaaagaaat tcattttaga
      241 atgattaaga gcagataatt gaagtaaagc tagatctacc ccaattgagg atttatgatt
      301 ccctattatt gcctccatta atgtaatata tattgaggta taaagaaata atattggtat
      361 ttttgtgtca tcaattgata acatcgtcat catataccaa aatagatagg aaagaagaat
      421 actaatcaaa ataaaagaaa aagttaaccc tttatttaac caacaaaaac gaagcaatat
      481 aaatagactg ctacgaaatc ttttttgact cattcgactg cttttaaaat agaaattact
      541 cgcatgatta tctaatatcc ccatgactaa aataaaataa ttgagtagta tgacataaat
      601 tacaacttgt ccttgttcta tagacaatag tcctatagat aaaaat
//
LOCUS       DAVMHG010000277          643 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVMHG010000277 DAVMHG010000000
VERSION     DAVMHG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.281"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..376
                     /locus_tag="VYR08_002152"
     CDS             20..376
                     /locus_tag="VYR08_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467595.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGWVGGILVII
                     GGSLFLATLKKFKSEE"
BASE COUNT          192 a           95 c          133 g          223 t
ORIGIN      
        1 attaaaaaca cttgctttaa ttagtggtat cgtcggtctt gtgggaggaa ttttacttct
       61 tattggtcct tttgtcttgt tgggaacagc ggtaaacaca gttgctacaa ctcttaatgg
      121 aggagctact gcaggggctt tttcaggtgt tgccttactc ttgaatgcct tgaagattgc
      181 aaatcttgtt cttggtatca ttgctattgt ttactataaa ggagataatc gtgtaggtgc
      241 agctccgtct gtactaatga ttgtttttgg tggagttagt ctcattccat tcttaggatg
      301 ggttgggggg attcttgtta ttatcggagg atctctattc cttgcaacat tgaagaaatt
      361 caaatcagaa gaataaaagg tattttagca tgaaaagaac aaaaaagttt atcggtatag
      421 gagtagctct attatctctt tttcttctag ttgcatgtgg aacataaagt tcaaaggata
      481 cttcaacaag taatgatgag aagacagtag caacatccaa tagttcaaaa gaaacaatca
      541 ctttcgatac accggttgta acagacgatg tgattgaatc aatacgcact tatgcagatt
      601 atatagatct ttataaaaat atttttgatg attattttac taa
//
LOCUS       DAVMHG010000278          641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVMHG010000278 DAVMHG010000000
VERSION     DAVMHG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.282"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(100..>641)
                     /locus_tag="VYR08_002153"
     CDS             complement(100..>641)
                     /locus_tag="VYR08_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV3467596.1"
                     /translation="LEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMEN
                     IVQSYLEAEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLY
                     LIEVNFYSGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDI
                     LPFLYNINDLEHNILKNLK"
BASE COUNT          230 a          128 c           61 g          222 t
ORIGIN      
        1 taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tttatcacaa attgattata
       61 aatacaatca atcccaattc ccatctaatt gaattggacc tatttcaaat tctttaaaat
      121 attatgttcc aaatcgttaa tattatataa gaatggtaaa atatcaaatg tttctcgtaa
      181 attattctta gccttatacc aaccttgtcc atcagtaatc cacataaact caacattagg
      241 aatagcttta atttcttcag caatcatttt gtaagatcta gccgtctcat ttaattttga
      301 accacttcct gaataaaaat taacctcaat cagataaagc acttgttcat tttttatgac
      361 aaaatcaaaa cgtttcacag tattcccatc attcgtaata gcagataaat cgacagaaaa
      421 aatttcctct atttcatttt gttcaatctc tttaaataaa ttctctccta aaatataccc
      481 ttcagcttca agataacttt gaacaatatt ttccatcgca tctccagtcc gatttttgcg
      541 cccattagta tccataccaa cctcaacacc tgtaacgtag tcaactaaat ttgatactaa
      601 atgattttgc aataaatcaa atatacctga tttttccaaa a
//
LOCUS       DAVMHG010000279          639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.283, whole genome shotgun sequence.
ACCESSION   DAVMHG010000279 DAVMHG010000000
VERSION     DAVMHG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.283"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>639
                     /locus_tag="VYR08_002154"
     CDS             <1..>639
                     /locus_tag="VYR08_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV3467597.1"
                     /translation="SGRNLFLNSLFKQDIPKTGIWTTSTYTATIDSESKYLGHKALKI
                     IGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNI
                     GYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEISDVD
                     TSYSEAPEDIEGQISTVESNFKQRADSLEAGVSRLTEGLRTKVDISALNVTA"
BASE COUNT          217 a          115 c          138 g          169 t
ORIGIN      
        1 atccggtcga aatctattct tgaactcact attcaagcag gatattccaa aaacaggaat
       61 ttggacaacg agtacatata cggctactat cgatagcgaa agtaagtatc ttggacacaa
      121 ggctcttaaa attataggtt tgaatccatc tggccgtgat ggaggtaatc ccaaggttac
      181 ttatccagct ctgggtcaat tcgggaaagt aattcccgga agtacgacta atcaagatgt
      241 aaccattagt ttttatgcta aggcaaataa aaatggaata atgctaagat ctcgattagg
      301 gaatatcgga tataaaactg gaaatgtgac attgtcgaca gaaattaagc gatatgttgt
      361 ccatattcca aaaggttgga caaacgaatc caagcagacc acaaatgaat ggttgttcaa
      421 tttcaaccag gaaggaacca tttggatttg gatgccgaag tttgaaataa gcgatgtaga
      481 tacttcttat tcagaagctc ctgaagatat agaaggtcag atttcaacag ttgaatccaa
      541 ctttaaacag cgtgctgatt cactcgaagc tggtgtaagc cgtctgactg aagggcttag
      601 aactaaagtc gatatcagcg cactcaacgt gactgctga
//
LOCUS       DAVMHG010000280          639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVMHG010000280 DAVMHG010000000
VERSION     DAVMHG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.284"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(58..130)
                     /locus_tag="VYR08_002155"
     tRNA            complement(58..130)
                     /locus_tag="VYR08_002155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Thr,seq:tgt)
     gene            complement(149..230)
                     /locus_tag="VYR08_002156"
     tRNA            complement(149..230)
                     /locus_tag="VYR08_002156"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Leu,seq:tag)
     gene            complement(236..308)
                     /locus_tag="VYR08_002157"
     tRNA            complement(236..308)
                     /locus_tag="VYR08_002157"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Lys,seq:ttt)
     gene            complement(333..405)
                     /locus_tag="VYR08_002158"
     tRNA            complement(333..405)
                     /locus_tag="VYR08_002158"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Asp,seq:gtc)
     gene            complement(408..480)
                     /locus_tag="VYR08_002159"
     tRNA            complement(408..480)
                     /locus_tag="VYR08_002159"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Val,seq:tac)
     gene            complement(485..600)
                     /gene="rrf"
                     /locus_tag="VYR08_002160"
     rRNA            complement(485..600)
                     /gene="rrf"
                     /locus_tag="VYR08_002160"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          164 a          179 c          135 g          161 t
ORIGIN      
        1 ggcaaggtgg tgttctacca ctgaactacg ttcgcactgt tttcttctat ctaaaaatgc
       61 cggctacatg acttgaacac gcgaccctct gattacaaat cagatgctct accaactgag
      121 ctaagccggc tcatttgtta tatcttaatg cgggttaagg gacttgaacc cccacgccgt
      181 taagcgccag atcctaaatc tggtgcgtct gccaattccg ccaaacccgc atatatgacc
      241 cgtactgggc tcgaaccagt gacccattga ttaaaagtca attgctctac caactgagct
      301 aacgagtcta aaataacttg cgttacctta aacggtcccg acgggaatcg aacccgcgat
      361 cttcgccgtg acagggcgac gtgataaccg ctacactacg ggacctttgg gagttaacgg
      421 gatcgaaccg ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaactccc
      481 taaagctaag cgacttccct atctcacagg gggcaacccc caactacttc cggcgttcta
      541 gggcttaact tctgtgttcg gcatgggtac aggtgtatct cctaggctat cgtcacttaa
      601 ctctgagtaa tacctactca aaattgaata tctattcaa
//
LOCUS       DAVMHG010000281          610 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVMHG010000281 DAVMHG010000000
VERSION     DAVMHG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.285"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..451)
                     /locus_tag="VYR08_002161"
     CDS             complement(<1..451)
                     /locus_tag="VYR08_002161"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEV3467598.1"
                     /translation="MQKPFKTIPEQIELLHKRGLIITDYKRAKKYLLTNNYYSIINGY
                     SKYFEQSPNKYRPGTNFSEITYSYFYDKEIKYSFLKAILEAEKHIKSAIAYVFSERFA
                     STPYFYLDINNYNKSNSDNVRKIKFVIRQMVKIIDKHKSSRQANSIQH"
BASE COUNT          184 a           83 c           84 g          259 t
ORIGIN      
        1 aatgttgaat actattcgct tgtctacttg acttatgttt atctattatt ttaaccattt
       61 gacggatcac aaatttaatc tttcgaacat tatcactatt cgacttatta tagttattta
      121 tatctaagta aaaataaggt gtagaagcaa atctttcaga aaatacatat gcaattgctg
      181 attttatatg tttttctgct tccaatattg cttttaaaaa agaatatttt atctctttgt
      241 cataaaaata actgtaagtg atttctgaaa agtttgttcc aggtctatat ttatttggtg
      301 actgttcaaa atatttactg tacccattga taatactgta gtaattgtta gttaatagat
      361 atttttttgc tcttttataa tctgtaatta ttaggccacg tttatgaagc aattctattt
      421 gttctggaat agttttaaat ggtttttgca aaaaaaagca cctccatata gaaggtgcta
      481 ttttcgcgtt cccgtttcac tgagaataat cgggcgctaa ttccttaagt accttcattg
      541 tatgataatt ttaaatgtta gtcaacagtt ttgttcaaaa taattgattt ttctttactt
      601 cgttttgttt
//
LOCUS       DAVMHG010000282          606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVMHG010000282 DAVMHG010000000
VERSION     DAVMHG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.286"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            107..553
                     /locus_tag="VYR08_002162"
     CDS             107..553
                     /locus_tag="VYR08_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3467599.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
BASE COUNT          205 a          134 c           95 g          172 t
ORIGIN      
        1 aaccatccag gatctaagct aaggcaagga ttctggatgg tttttagatt tggggtgaat
       61 aattggggtt tacctcttct tccctactca cctgagtaca atcctattga gaaaacatgg
      121 gctcatatca aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttacgaggct
      181 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      241 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      301 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      361 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      421 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      481 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
      541 aaagattttt taaaaagttt tgcccctttt ttgtcctcta aatacaaaaa tagcccttcg
      601 gataaa
//
LOCUS       DAVMHG010000283          603 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVMHG010000283 DAVMHG010000000
VERSION     DAVMHG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.287"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..603
                     /locus_tag="VYR08_002163"
                     /pseudo
     CDS             1..603
                     /locus_tag="VYR08_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a          111 c          112 g          167 t
ORIGIN      
        1 aaaacaggag agctaaacca ccaagtaaaa ggaacaaaac caagaaaagt tgatagagat
       61 agacttaaaa actatcttac tgacaatcca gatgcttatt tgactgaaat agcttctgac
      121 tttggctgtc atccaactac catccactat gcgctcaaag ctatgggcta cactcgaaaa
      181 aaagaaccac acctactatg aacaagaccc agaaaaagta gccttatttc ttaagaattt
      241 taatagatta aagcacctag cacctgttta gattgacgaa acaggattcg atacttattt
      301 ttatcgagaa tatggtcgct cattaaaagg tcagttaata agaggcaaag tatctggaag
      361 aagatatcag agaatttctt tggttgcagg tctaacaaat gaatggtgag ttaatcgctc
      421 caatgactta cgaagagacg atgacgagcg acttttttga agcttggttt cagaagtttc
      481 tcttaccaac attaaccaca ccatcggtta ttattatgga taatgcaaga ttccatagaa
      541 tggggaagct agaactcttg tgtgaagagt ttgggtataa acttttacat cttccttcct
      601 act
//
LOCUS       DAVMHG010000284          593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVMHG010000284 DAVMHG010000000
VERSION     DAVMHG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.288"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..573)
                     /locus_tag="VYR08_002164"
     CDS             complement(<1..573)
                     /locus_tag="VYR08_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467600.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQSYLGGNTA
                     WIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAIFENLLDSY
                     LLQNQSEVDLDIFYKVLPLLYKEKYTMGFNY"
BASE COUNT          214 a           90 c           91 g          198 t
ORIGIN      
        1 gtaattgaat cccatagtat atttttcttt ataaagcaaa ggtaaaactt tataaaaaat
       61 atctaagtca acctcactct gattctgaag caagtaggag tcaagcaaat tttcaaaaat
      121 agctaacaat atgattggtg taaggttttt gtattcacat gtcaagtatt ctgagtatcg
      181 tgcagatgat atttctttta tataactttt tgaaagcaag attttttcaa attcatcatc
      241 tattattaat cttctatttc caatccaagc ggtattacca cccaaataag attgtgcaaa
      301 tagcaatatg taagaaaata tgaacgtaaa tactgaaaga ggtagaattc tatgcgtaaa
      361 aaaatagacg ataatggtag ccaggagtaa agatatctct cgtgacaatc gaattctttt
      421 cagcatcttt tttaacagtc cgtaaggttg tccttggcaa tattcttgaa ctaaattaat
      481 cggaatgaca tctctcacca tttctggaaa gtagagtaag tttataggtt gaaatctcca
      541 gctcccatca taagtaaatg gatatagaac caaatactta aaagattttt tta
//
LOCUS       DAVMHG010000285          593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVMHG010000285 DAVMHG010000000
VERSION     DAVMHG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.289"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..178
                     /locus_tag="VYR08_002165"
     CDS             <1..178
                     /locus_tag="VYR08_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV3467601.1"
                     /translation="VNEDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVP
                     VFDNIRLPAKQNMV"
     gene            186..593
                     /locus_tag="VYR08_002166"
                     /pseudo
     CDS             186..593
                     /locus_tag="VYR08_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          198 a          112 c          118 g          165 t
ORIGIN      
        1 tgtaaacgaa gaccttatga aaggttacga tatctttaca ccaatcgctg caacagacct
       61 tggttttgaa ccaggtatcc cagttatcga agctgggcca atcttattct gtatcccagc
      121 aatgagcgtt ccagtatttg acaatattag acttcctgcg aaacaaaata tggtatagta
      181 gttccatgaa ttatgaagca agtaaacaac taactgatgc acgatttaag cgtcttgttg
      241 gtgttcaacg cacgactttt gaagagatat tagctgtatt aaaaacagct tatcaactta
      301 aacacgcaaa aggtggacga aaacctaaat taagcctaga agactttctt atggccactc
      361 ttcaatatgt gcgagaatat cgaacttatg aagaaattgc ggctgatttt ggtatccacg
      421 aaagcaactt aatccgtcgg agccgatggg ttgaagtaac tcttgttcaa agtggtgtta
      481 cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg acggaagtac
      541 aaatcaatcg ccctaaaaaa acaattagcg aattattctg gtaaaaagaa att
//
LOCUS       DAVMHG010000286          591 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.290, whole genome shotgun sequence.
ACCESSION   DAVMHG010000286 DAVMHG010000000
VERSION     DAVMHG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.290"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..402
                     /locus_tag="VYR08_002167"
     CDS             22..402
                     /locus_tag="VYR08_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV3467602.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            415..>591
                     /locus_tag="VYR08_002168"
     CDS             415..>591
                     /locus_tag="VYR08_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV3467603.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPW"
BASE COUNT          198 a           96 c          111 g          186 t
ORIGIN      
        1 caaatttgtt ggaggtctcc catgattgat ctctatctaa gtaaaaatag ccaaagaaat
       61 caacttcttt tagacttctt ccaaaactat ggcattgagg tatcttgtca ttcagtttct
      121 gaaatgacaa aggacaaatt aattgagatg atgagctatt cttcagattg ttttgagttt
      181 ttatctccca atttattacg atttaagaac cgtgacaatc taagattaac ggatttcgtt
      241 gagataattt taaaaaatcc tgaactaacc atcggacttc cttttgtggt ttcaaataag
      301 cgggtttatc caagtctgaa tttggaagag gctagagctc tattgccaag agatacgaaa
      361 caattgattt acatggcaca aacacattat ttatctaact aaaggagaag taatatggct
      421 gaacgatttt gggagaactt gtccattatt ttggctgaaa gaaatatcag ctggattgag
      481 ttaaccagaa aaatgtttgc gggagagttt cactatccaa gtgaattgaa tcgtttatat
      541 caaaagattc gccactacaa gatggaacaa cgaatgcctc aaagtccatg g
//
LOCUS       DAVMHG010000287          583 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.291, whole genome shotgun sequence.
ACCESSION   DAVMHG010000287 DAVMHG010000000
VERSION     DAVMHG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.291"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>583
                     /locus_tag="VYR08_002169"
     CDS             <1..>583
                     /locus_tag="VYR08_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV3467604.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWF
                     DEGLYEAPKGYTLEDLLATVKYYVEHPNERPHS"
BASE COUNT          185 a          124 c          128 g          146 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagctaagaa ggtgccactt gatcgtatgc cttacaatct tcaatatact gtagaagtca
      421 aaaacggtag tttaatcata cctcattatg accattacca taacatcaaa tttgagtggt
      481 ttgacgaagg cctttatgag gcacctaagg ggtatactct tgaggatctt ttggcgactg
      541 tcaagtacta tgtcgaacat ccaaacgaac gtccgcattc aga
//
LOCUS       DAVMHG010000288          562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVMHG010000288 DAVMHG010000000
VERSION     DAVMHG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.292"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..279
                     /locus_tag="VYR08_002170"
     CDS             43..279
                     /locus_tag="VYR08_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467605.1"
                     /translation="MNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKL
                     PKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            263..559
                     /locus_tag="VYR08_002171"
     CDS             263..559
                     /locus_tag="VYR08_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467606.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
BASE COUNT          243 a           56 c          105 g          158 t
ORIGIN      
        1 cagaagaacg gagagtttaa ctcaagtaaa gataatagac aaatgaatga caagatcgac
       61 aataaacaag ataataagac agaagaagta aactataaaa tagttggaga tggcagagaa
      121 actgaaaatc atattaataa atctaaagaa atagtagatg taaaacaaaa attaccaaag
      181 actggttcga acaagattat ggaactattc ttaacagtga caggaattgg tttacttttg
      241 acactaaaag ggttgaagta ttatggtaaa gataaataaa atttgttcga tacaaggaag
      301 ttccgtagaa aatgaggata ttgtaggttc acaaaatcag tatttttgga ttattggtgg
      361 agctacagat ttatataatt ctaaagagga gataggttat tcagtctcag aagttgtaca
      421 tattttatca gaaagtttgt cggttaattg taaagaatca aaaactctta aacaaatatt
      481 tgaaactgct ttactcgagg ttaaagatga aatcggttta aactcatata aattaacaga
      541 gtatagtaaa atgaaataat aa
//
LOCUS       DAVMHG010000289          556 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVMHG010000289 DAVMHG010000000
VERSION     DAVMHG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.293"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>556
                     /locus_tag="VYR08_002172"
     CDS             <1..>556
                     /locus_tag="VYR08_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV3467607.1"
                     /translation="LPSNITQSKGFKIHLSASILNANLVAKKFFDFIFSREKKINFKI
                     LVSIKELSLQNTGLNGYSQVGKFITIYPKDNKEFQRLLHKLEILYKGVKGVNIPSDFR
                     FQLSEVVYYRYGEFVKDSTFKDKRDKKIPSNVNVPIRDYYIPRYNTIPDQYIILEVIS
                     KNAKGGVYKVFNTQKRVYSLLKEA"
BASE COUNT          210 a           71 c           92 g          183 t
ORIGIN      
        1 atttgccgtc taatatcacc caatctaagg gatttaaaat acacctgagc gcttcaatat
       61 taaatgctaa tttggttgct aaaaaattct ttgattttat attttctaga gagaagaaga
      121 taaatttcaa aattttggtt agcattaaag aactttcttt gcaaaataca gggcttaatg
      181 gttattcaca agtcgggaaa ttcattacaa tttatccaaa agataataaa gagtttcaac
      241 gtttgttaca caaattagaa atattgtata aaggtgtaaa gggtgtaaac ataccatcag
      301 atttcagatt tcaacttagc gaggttgtat attacagata tggggagttc gtaaaagata
      361 gcactttcaa ggataaaaga gataagaaaa tcccttcaaa tgtgaatgtt ccaataaggg
      421 attactatat tccaagatat aacactattc ctgatcaata tattattttg gaagtgattt
      481 ctaaaaatgc caaaggtgga gtttataaag tatttaatac tcaaaagcga gtttattctt
      541 tattaaaaga agctag
//
LOCUS       DAVMHG010000290          555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVMHG010000290 DAVMHG010000000
VERSION     DAVMHG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.294"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..250
                     /locus_tag="VYR08_002173"
     CDS             <1..250
                     /locus_tag="VYR08_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV3467608.1"
                     /translation="IVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGEN
                     KYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            325..522
                     /locus_tag="VYR08_002174"
     CDS             325..522
                     /locus_tag="VYR08_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV3467609.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          192 a          100 c           74 g          189 t
ORIGIN      
        1 tatcgtttta agaatcccag ttattcctaa ttttaacaat agtttagagg atgcagaaaa
       61 attcgctact ctatttaact cattaaatat cgaccaagtt caactactcc cttttcatca
      121 atttggtgaa aacaaatatc gtttattaaa tcggaaatat gaaatggatg gagtcaacgc
      181 acttcatcct gaagatctta ttgattatca aaaggtattt ctgaaccacc atattaattg
      241 ttatttctag tttatttcct tgaaatgctc tagctatttg cagataacaa gcatctatat
      301 aaggttatca aagaagactt ccccatgact ctaaatgaat atatcttaca atatcgcctg
      361 aaacaagcta tagataagat ggctgaatct cccaactcct ctttaagcga tatctctgac
      421 caagttggat tttcagacta taaatatttt gccaaagtat ttaaaaagta tctccatatt
      481 tccccaaaga aattgaaatc actcggaaga atagtaaaat aggctattct tctttttgta
      541 gagaatttta ctcta
//
LOCUS       DAVMHG010000291          553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.295, whole genome shotgun sequence.
ACCESSION   DAVMHG010000291 DAVMHG010000000
VERSION     DAVMHG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.295"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..358
                     /locus_tag="VYR08_002175"
                     /pseudo
     CDS             1..358
                     /locus_tag="VYR08_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          197 a           87 c          106 g          163 t
ORIGIN      
        1 aagctatggg ctacactcga aaaaaaagaa ccacacctac tatgaacaag agccagaaaa
       61 agtagcctta tttcttaaga attttaatag tttaaagcac ctagcacctg tttagattga
      121 tgaaacagga ttcgatactt atttttatcg agaatatggt cgctcattaa aaggtcagtt
      181 aataagaggc aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac
      241 aaatggtgaa ttaatcgctc caatgactta cgaagagacg atgacgagcg acttttttga
      301 agcttggttt cagaagtttt tcttaccaac attaaacaca ccatcggtta ttatagtaaa
      361 atgaaataag aataggacaa atcgatcagg acagtaaaat cgaattctaa caatgtttta
      421 gaagtagagg tgtactattt tagtttcaat ctactatatt ctcaaatttc ctacgcccgc
      481 gatggacaat gcaagattcc atagaatggg taagctagaa cttttatgtg aagagtttgg
      541 gcataaactt tta
//
LOCUS       DAVMHG010000292          529 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.296, whole genome shotgun sequence.
ACCESSION   DAVMHG010000292 DAVMHG010000000
VERSION     DAVMHG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.296"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..266
                     /gene="pmp23"
                     /locus_tag="VYR08_002176"
     CDS             <1..266
                     /gene="pmp23"
                     /locus_tag="VYR08_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV3467610.1"
                     /translation="YNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTYS
                     YIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            340..>529
                     /locus_tag="VYR08_002177"
     CDS             340..>529
                     /locus_tag="VYR08_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV3467611.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNA"
BASE COUNT          164 a          112 c           91 g          162 t
ORIGIN      
        1 cctataattt tggacctgcc tatatcgatt ttatcgccca aaatggcaag gaaaatacct
       61 tggctctagc caaacagtac tctcgtgaga ctgttgcccc cttgcttggt aatacgactg
      121 gaaagactta tagttatatt caccccattt ccatttttca cggtgctgaa ctctatgtaa
      181 atggaggaaa ctattattat tctagacagg tacgacttaa cctttacatc atcaaatgtt
      241 tcactctctt ttcaacatct ggctagacca ggtgtttttg ttataagttt tctttaagat
      301 agatatatta ctctaaagaa gtaaaggagg aaattcccta tgagaaagaa actctttctg
      361 actagcgctg cgatcttgtg ggcagtaaca gctatgaata gcgtccatgc agcaacagat
      421 gttcaaaaag ttatcgatga aacctatgtc caacctgaat atgtcctagg ttcttcccta
      481 tctgaagacc aaaaaaatca aactcttaaa aaactgggct acaatgcct
//
LOCUS       DAVMHG010000293          523 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.297, whole genome shotgun sequence.
ACCESSION   DAVMHG010000293 DAVMHG010000000
VERSION     DAVMHG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.297"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..171
                     /locus_tag="VYR08_002178"
     CDS             <1..171
                     /locus_tag="VYR08_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV3467612.1"
                     /translation="EELKSLGKWYVSTGKEWICHSDYELEEFKNIFLNFINPEEWDTI
                     SFDSDFMPFQQS"
     gene            298..465
                     /locus_tag="VYR08_002179"
     CDS             298..465
                     /locus_tag="VYR08_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467613.1"
                     /translation="MHSFEVADKAIGQKVFDTEKRIISGVENLDTMRFIVGRFTSEIE
                     LLPRIFQLIKA"
BASE COUNT          180 a           77 c           94 g          172 t
ORIGIN      
        1 gaagaattga aaagtcttgg aaaatggtat gtctctaccg gtaaagaatg gatttgtcat
       61 tcagactatg agctggaaga atttaaaaat atctttttaa attttatcaa tcctgaagaa
      121 tgggatacta tctcctttga ttcagatttt atgccgtttc aacaatcgta accaatttcc
      181 aaaaaaagtt aaatcttata tctagtactc tgcaaaactc ttatctaatc acgttgctta
      241 tactcaataa aaatcaaaga gcaactttaa actaggaagc gagtcgcagg tttctcaatg
      301 catagctttg aggttgcaga taaagcaatt gggcaaaaag tctttgatac agaaaaacgc
      361 ataatatcag gtgttgaaaa ccttgatact atgcgtttta ttgtgggaag atttacttct
      421 gaaattgagt tgttacccag aatctttcag ttgattaagg cttgatgact ttagtatgtt
      481 tagatagctc aaaaaggatt gaatcactta gtttagaatc tga
//
LOCUS       DAVMHG010000294          522 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.298, whole genome shotgun sequence.
ACCESSION   DAVMHG010000294 DAVMHG010000000
VERSION     DAVMHG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.298"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>522
                     /locus_tag="VYR08_002180"
     CDS             <1..>522
                     /locus_tag="VYR08_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HEV3467614.1"
                     /translation="TIIFDGLDEVNPSKRSWLDKEIKEFVTLYNSNRYVLSSRPSEEF
                     IGWNQFAEYEIKKLNREQALSLIDKLNYDEKVKRRFYRELKDHLYDTHESFASIPLLL
                     TIMLMTYEAGASIPNNLTDFYNQAFYTLYQRHDASKSGYKRELKAKLTPEEFRNILAY
                     IGLKTFFEGKVDFD"
BASE COUNT          192 a           68 c           89 g          173 t
ORIGIN      
        1 acaataattt ttgatggttt agatgaggtt aatccttcaa aacgttcttg gttggacaaa
       61 gaaattaaag aatttgtaac gttatacaat agcaatagat atgttttgtc gtcaagaccg
      121 tctgaggaat ttattggttg gaatcagttt gctgagtatg agataaaaaa attaaataga
      181 gagcaagcgt tatccttaat tgataagttg aattatgatg agaaagtaaa aaggcgtttc
      241 tacagagaat taaaggatca tttatatgat actcatgaat cttttgcttc tatcccactc
      301 ctattaacaa ttatgttaat gacttacgaa gctggtgctt ctattccaaa taatttaact
      361 gatttttata atcaggcctt ttatacattg taccaaagac atgatgcatc taaatcagga
      421 tacaaaagag aactaaaagc aaaattaact cctgaagaat ttcgaaatat attagcctac
      481 attggtttaa aaacattctt tgaaggaaaa gtagatttcg at
//
LOCUS       DAVMHG010000295          515 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.299, whole genome shotgun sequence.
ACCESSION   DAVMHG010000295 DAVMHG010000000
VERSION     DAVMHG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.299"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            149..515
                     /locus_tag="VYR08_002181"
                     /pseudo
     CDS             149..515
                     /locus_tag="VYR08_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390504.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          206 a           73 c           73 g          163 t
ORIGIN      
        1 aaaagcagaa ttaattacaa gaataattaa taatgttaac attgacaatt tagatattcc
       61 gttcgaattt aaactaacaa aagaagcaca aaatcttatt atcgaacata gtgactatat
      121 caaagcatac tatgataaag acataactat ggaagattat tgtaaagaaa aaacaatatc
      181 tcttttaaag caacttttgg tgatataaaa tggagtctct taaataaaca agctcatagg
      241 aatactgtat caggagattt tggatgctta tctaacacac gaaaggctca gggaagacat
      301 ttggaacaag aaggtaatat taaacatgct ttaatatatt acatagaatc tttgataatt
      361 actatttcag gattagaaaa caatttttca gccactgatt atccagtata ttatcccgat
      421 tcgatacctg actactcact aaaacatatt caaacattaa tggaatcatt atctgatgac
      481 gattatgatt ttgcttttga tgaagcatta tttcg
//
LOCUS       DAVMHG010000296          495 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.300, whole genome shotgun sequence.
ACCESSION   DAVMHG010000296 DAVMHG010000000
VERSION     DAVMHG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.300"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(80..153)
                     /locus_tag="VYR08_002182"
     tRNA            complement(80..153)
                     /locus_tag="VYR08_002182"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Ile,seq:gat)
     gene            complement(188..258)
                     /locus_tag="VYR08_002183"
     tRNA            complement(188..258)
                     /locus_tag="VYR08_002183"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Gly,seq:tcc)
     gene            complement(264..336)
                     /locus_tag="VYR08_002184"
     tRNA            complement(264..336)
                     /locus_tag="VYR08_002184"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Val,seq:tac)
     gene            complement(341..456)
                     /gene="rrf"
                     /locus_tag="VYR08_002185"
     rRNA            complement(341..456)
                     /gene="rrf"
                     /locus_tag="VYR08_002185"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          141 a          134 c          105 g          115 t
ORIGIN      
        1 atagtttata cgggaacaag cccgtgtcct cttagtgaac agataatatt ccaatcccct
       61 agtaatggac taaacactat gggcacgagt ggactcgaac caccgacctc acgcttatca
      121 ggcgtgcgct ctaaccacct gagctacgcg cccaagtcaa aaacttggta caaagaacaa
      181 agttcaaagc gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtagtttta
      241 ccactaaact acacccgcta aaatgggagt taacgggatc gaaccgctga ccctctgctt
      301 gtaaggcaga tgctctccca gctgagctaa actccctaga gctaagcgac ttccctatct
      361 cacagggggc aacccccaac tacttccggc gttctagggc ttaacttctg tgttcggcat
      421 gggtacaggt gtatctccta ggctatcgtc acttaactct gagtaatacc tactcaaaat
      481 tgaatatcta ttcaa
//
LOCUS       DAVMHG010000297          485 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.301, whole genome shotgun sequence.
ACCESSION   DAVMHG010000297 DAVMHG010000000
VERSION     DAVMHG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.301"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>485
                     /locus_tag="VYR08_002186"
     CDS             <1..>485
                     /locus_tag="VYR08_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467615.1"
                     /translation="KDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLNLAGKFGK
                     NAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDEPTVGLDT
                     ESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDMRDFVDNS
                     T"
BASE COUNT          164 a           68 c           88 g          165 t
ORIGIN      
        1 taaagatttg atagttagtt ctcaaccttt tgctcattta ggctttactc ctcaaaccac
       61 agtaattgat ttttatacta ctgtgaagga caatgtaata ttggggctga accttgctgg
      121 aaagtttggg aaaaatgctg agaagttgtg tcaaatagcc ttagaaattg ttgggttagc
      181 tgataaaaaa aataatttgg tagaaacatt gtcaggtgga caactgcaac gcgtccagat
      241 tgctagagca atagctcata atccagattt ttatatttta gatgaaccta ccgttggttt
      301 agatactgaa tctgccgaaa aatttttaat gtatttaaaa gataagagtt tggaaggaaa
      361 aactattatc atatcttcac atgacataaa tctactcgaa aagttttgta aaaaaatact
      421 ttttttacaa aatggctcca tatcattttt tggtgatatg cgtgactttg tagataattc
      481 aacta
//
LOCUS       DAVMHG010000298          484 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.302, whole genome shotgun sequence.
ACCESSION   DAVMHG010000298 DAVMHG010000000
VERSION     DAVMHG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.302"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            132..>484
                     /locus_tag="VYR08_002187"
     CDS             132..>484
                     /locus_tag="VYR08_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV3467616.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVME"
BASE COUNT          259 a           67 c           69 g           89 t
ORIGIN      
        1 agaaatatat aaaaacatca aaataaagta ttccaccacc aagaacaata aaataaaaac
       61 aagcaaaggg aagtatagaa aaattaaaac ctatacttcc cttttttaat tcaaacaaag
      121 gagagaaaag catgataaac gaagaaataa gcaaggaagc aggtcaagca gcacaaacca
      181 taatatcata cacaataaag gcaacaaaag aatcaatcaa tttagaaaaa gaaataagaa
      241 aaaagatgaa tgaaacttta gaaaaagcaa atggaaactt aaaaagtctt atgggcgatg
      301 aaatgaaaat aaaagatctc tacaagaaag gacaactaga aaatataagc atagatcaaa
      361 tcgacctcaa agacttaaaa aaagaactaa acaaacttgg agtaagtttc tcagtaatga
      421 aaaacaaaga aagcaaaaac tatgaaatat tcttccaagc caaagacata aaagtaatgg
      481 aata
//
LOCUS       DAVMHG010000299          480 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.303, whole genome shotgun sequence.
ACCESSION   DAVMHG010000299 DAVMHG010000000
VERSION     DAVMHG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.303"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            276..>480
                     /locus_tag="VYR08_002188"
     CDS             276..>480
                     /locus_tag="VYR08_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467617.1"
                     /translation="MDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELI
                     KMRFNSSAIDPIYGKKDDASILVI"
BASE COUNT          185 a           51 c           86 g          158 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaacatac tatattatcg cttaccaata tttgctaaac
       61 tttcagaaaa aaagttggag tatatgatgc taggtgactg cgtcatgtta gtaaacgaaa
      121 tggaaataac tgatcataga gtagataact tatttgaaaa agggaaaaat gagataaaag
      181 attcaattgg tacaaatagt gttttaaata aaaaaattat tttacaaaaa atcagaaaat
      241 tatctaatca accgagtggg tactggatag gttcgttgga tgagagattt ttagaccatg
      301 ctattattaa tcaaatagat gttactagtg aacaaattgt tttaatgagc gatggctttt
      361 atgaattcta tcaaaataat caaaataaaa cttttgaaga gttaattaag atgagattta
      421 attcttctgc tattgatcca atctatggaa agaaggatga tgcaagtatt ctagtcattg
//
LOCUS       DAVMHG010000300          479 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.304, whole genome shotgun sequence.
ACCESSION   DAVMHG010000300 DAVMHG010000000
VERSION     DAVMHG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.304"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..200
                     /locus_tag="VYR08_002189"
     CDS             <1..200
                     /locus_tag="VYR08_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467618.1"
                     /translation="INHIKYSVDFVNKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQD
                     QVNKDNEYREMMKNKKMDQER"
     gene            274..447
                     /locus_tag="VYR08_002190"
     CDS             274..447
                     /locus_tag="VYR08_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467619.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
BASE COUNT          242 a           58 c           77 g          102 t
ORIGIN      
        1 aaattaatca tataaaatat tcagtagatt ttgtaaacaa agaacttggt atagatttat
       61 ccatagaaat agataagcta ataaaacagg gagaaaaacc aagtgtaata gcacagatta
      121 aaaagttcca agaccaagtc aataaagata atgaatacag agaaatgatg aaaaacaaga
      181 aaatggatca agaaagataa gacctggtaa aaatatctta tccaagctat aatatgacca
      241 aacaactaaa gtcagatcta ggaggtaata gagatgaata aaaggcaaga gctaatagat
      301 gaactcataa aagcaaatga agatggaaca tacaaaacct ataaaagcac agaagaaata
      361 aaagcaatga acaatgaaga aatacagatt atatattcca acatgaaaaa ctatctatca
      421 gacaaaagaa cacacataaa ctattaaagg tggaaaggta gtgtaagcta tttttaaaa
//
LOCUS       DAVMHG010000301          466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.305, whole genome shotgun sequence.
ACCESSION   DAVMHG010000301 DAVMHG010000000
VERSION     DAVMHG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.305"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..430
                     /locus_tag="VYR08_002191"
     CDS             <1..430
                     /locus_tag="VYR08_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV3467620.1"
                     /translation="ADNQEHLFDQVATLLEEREIVTPTYREALITREKSFPTGLDMEF
                     LGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSS
                     SQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
BASE COUNT          159 a           84 c           84 g          139 t
ORIGIN      
        1 agcggataat caagaacatc tctttgatca ggttgcaact ttattggaag aacgagaaat
       61 tgtaactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc caactggttt
      121 agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcaca cagatattgt
      181 tcataatcta gctgagaaag tggtggttgt tcgattagaa aaaccagtaa cttttcacaa
      241 tatgatagct cctgataagg aagtagaagt atctctactc ttctttatca ttaacaattc
      301 aagttcaagt caaacgaaca ttctggctca gttgatggac tttttcacag gaaatggtca
      361 tcttgaagac ctatcaaaaa tttctgaacc agaaaaactt tatgcttaca ttgctgaagc
      421 aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatcca
//
LOCUS       DAVMHG010000302          465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.306, whole genome shotgun sequence.
ACCESSION   DAVMHG010000302 DAVMHG010000000
VERSION     DAVMHG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.306"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..429
                     /locus_tag="VYR08_002192"
                     /pseudo
     CDS             1..429
                     /locus_tag="VYR08_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT          157 a           78 c           91 g          139 t
ORIGIN      
        1 agcggataat caagaataac tttttgatca ggtagcaagc ttgttggaag aacgagaaat
       61 tgtcactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc caactggttt
      121 agatatggaa tttctaggaa aggacttgca aatgtaatga ttcctcacac agatattgtt
      181 cataatctag ctgagaaagt ggtggttgtt cgattagaga aaccggtgac tttccataat
      241 atgattgcgc caggtaagga agtagaagta tccttgctct tctttatcat caataattca
      301 agttcaagtc aaacgaacat tcttgctcag ttgatggact ttttcacagg aaatggacat
      361 cttgaagacc tatcaaaaat ttccgaacca gaaaaacttt atgcttatat agatgaagca
      421 atcgcttaat cttgtctatt aaaaaataaa atcggaggaa gtcta
//
LOCUS       DAVMHG010000303          463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.307, whole genome shotgun sequence.
ACCESSION   DAVMHG010000303 DAVMHG010000000
VERSION     DAVMHG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.307"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..238
                     /locus_tag="VYR08_002193"
     CDS             20..238
                     /locus_tag="VYR08_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467621.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          197 a           52 c           91 g          123 t
ORIGIN      
        1 aagaaaaaag agggatatta tggcacttaa ctataaacca ttatggatac agttagcaaa
       61 aaaaggacta aagaaaacag atgtaatagc tatggcagga cttacaacaa atgttatggc
      121 acaaatggga aaggataaac caattacatt taagaattta gaaagaatat gtaaggcttt
      181 atcttgcact cctaatgata ttattagttt tgaagataat tttagtgacg aggaatagaa
      241 aatgacttta aggacagaag atcaagttag ggattatgca agagaagtat aggctttaat
      301 gaagttgaag aaaacatcaa tcaaggtact ggtcaaataa ctacttttaa tcaattaggc
      361 ttcaagggat attcagataa gccagatggt tggtatttac ctaaaaatat gaatgatgta
      421 gcaataatcc ttgaaacaaa atcagaagaa agagatatta gca
//
LOCUS       DAVMHG010000304          452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.308, whole genome shotgun sequence.
ACCESSION   DAVMHG010000304 DAVMHG010000000
VERSION     DAVMHG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.308"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>452)
                     /locus_tag="VYR08_002194"
     CDS             complement(<1..>452)
                     /locus_tag="VYR08_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV3467622.1"
                     /translation="HFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGR
                     TQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRA
                     MSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRM"
BASE COUNT          147 a           83 c           89 g          133 t
ORIGIN      
        1 tgcatacgaa aagtagggcg ataaaaatgt ttatcgctga gtttacgact atcctgttgt
       61 atgagcttcc agtagcgctt gatagccttg tattcatgag actttcgatc caattgattc
      121 atgatttgaa cacgcacacg actcatagca cggctaagat gttgtacaat gtgaaagcga
      181 tccaacacga ttttagcaca tgggaaaagt tgtctagcta agtcatagta aggactaaac
      241 atatccatag taataatttt gacgcgacat cggacggctc tatcatattt aagaaagtga
      301 tttcgaatga tagcttgtgt tcttccctca agaacagtga tgatattgag attgttaaaa
      361 tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat actcatccca agacataatc
      421 tcaggaagac aagaaaaatc atgtttaaag tg
//
LOCUS       DAVMHG010000305          447 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.309, whole genome shotgun sequence.
ACCESSION   DAVMHG010000305 DAVMHG010000000
VERSION     DAVMHG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.309"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..415)
                     /locus_tag="VYR08_002195"
     CDS             complement(<1..415)
                     /locus_tag="VYR08_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV3467623.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
BASE COUNT          137 a           84 c           66 g          160 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccggac
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctat
//
LOCUS       DAVMHG010000306          438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.310, whole genome shotgun sequence.
ACCESSION   DAVMHG010000306 DAVMHG010000000
VERSION     DAVMHG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.310"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            212..>438
                     /locus_tag="VYR08_002196"
     CDS             212..>438
                     /locus_tag="VYR08_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV3467624.1"
                     /translation="MPLFGTPGFYETNLNLLNNQPEDIMGLVLLLYWSQNLDDFNVFS
                     KMSFLEAMEYAMNFNSRRLDTLDKSDWLYDI"
BASE COUNT          156 a           47 c           75 g          160 t
ORIGIN      
        1 tgcagataaa ttaagcgagt ataatatggt tagtggctcg tatgattggt ttttaacatt
       61 aattgattat atggagataa taaactccaa atataatctt tcctatagag atttatcatt
      121 taataatata cttattgata atagaaataa tatttatatt atagactttg agcatgccct
      181 aaggaaagaa actcaaattg aagaaaaaaa gttgcctcta tttggaactc cagggtttta
      241 tgaaaccaac ttaaatttat tgaataatca accagaggat attatgggat tggttttgtt
      301 attatattgg agtcagaatt tagatgattt taacgtcttt tcaaaaatga gttttttaga
      361 agcgatggag tatgcaatga attttaattc gcgtcgctta gacacacttg ataaatcaga
      421 ttggctttat gatattta
//
LOCUS       DAVMHG010000307          435 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.311, whole genome shotgun sequence.
ACCESSION   DAVMHG010000307 DAVMHG010000000
VERSION     DAVMHG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.311"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>435
                     /locus_tag="VYR08_002197"
     CDS             <1..>435
                     /locus_tag="VYR08_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467625.1"
                     /translation="KSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQA
                     PSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLL
                     SDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIV"
BASE COUNT          163 a           69 c           58 g          145 t
ORIGIN      
        1 taaaatcaat ttattggaat tttgggcaaa ataagccaga gaaatccttt aggtatatag
       61 atacgtctag tattgataga aaaaagaaca taatcaacta caaaaatcta caatatcttt
      121 cacctgaaca agcgccttcc cgtgccagaa aattagtttc gcagaatagt gtcttatttt
      181 caacagttag accatatcta aaaaatattg ctgtagttag agaacttaaa gagtatttga
      241 tagctagtac agcatttatt gttttggata ctttacttaa cgaaacatat ttgaaatatt
      301 atttattatc agataatttt atcaatcgtg ttaataataa atctactgga acaagttatc
      361 ctgcaatcaa tgattataat tttaatctat tattaattgc tctcccccca ctatccgaac
      421 aacaacgaat agtag
//
LOCUS       DAVMHG010000308          432 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.312, whole genome shotgun sequence.
ACCESSION   DAVMHG010000308 DAVMHG010000000
VERSION     DAVMHG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.312"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(8..81)
                     /locus_tag="VYR08_002198"
     tRNA            complement(8..81)
                     /locus_tag="VYR08_002198"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VYR08_002199"
     tRNA            complement(95..168)
                     /locus_tag="VYR08_002199"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VYR08_002200"
     tRNA            complement(174..247)
                     /locus_tag="VYR08_002200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VYR08_002201"
     tRNA            complement(263..336)
                     /locus_tag="VYR08_002201"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VYR08_002202"
     tRNA            complement(346..431)
                     /locus_tag="VYR08_002202"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
BASE COUNT          115 a          125 c          104 g           88 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cc
//
LOCUS       DAVMHG010000309          427 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.313, whole genome shotgun sequence.
ACCESSION   DAVMHG010000309 DAVMHG010000000
VERSION     DAVMHG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.313"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..243
                     /locus_tag="VYR08_002203"
     CDS             <1..243
                     /locus_tag="VYR08_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV3467626.1"
                     /translation="DYYRFITHIKLFAHRLVENTTYCDDDDEDLLALMKNKYPREYEC
                     GEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
     gene            261..>427
                     /locus_tag="VYR08_002204"
     CDS             261..>427
                     /locus_tag="VYR08_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HEV3467627.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     MKKIPGVIGIM"
BASE COUNT          167 a           44 c           81 g          135 t
ORIGIN      
        1 gattattata gattcataac tcatataaaa ttatttgccc atcgcttggt tgaaaataca
       61 acttattgtg acgatgatga tgaagacttg ttagcattaa tgaaaaataa atatcctaga
      121 gaatatgaat gtggtgaaca ggtggcaatg tttatacaaa ctgagtacaa ttatttgctg
      181 acttctagtg aattagttta tttgatggct catattcgtc gcttaacaaa aaatttagat
      241 taatagaata ggagaatacc atgagttata aagataaggt acaaaaaatc ctcgatgtaa
      301 ttggaggtga aaaaaatgtc aatagagtta cccattgtgt aacacgttta agattagaat
      361 taaaagatga aaatttagtc aatgatgatg atatgaagaa gataccaggt gtaataggta
      421 ttatgaa
//
LOCUS       DAVMHG010000310          426 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.314, whole genome shotgun sequence.
ACCESSION   DAVMHG010000310 DAVMHG010000000
VERSION     DAVMHG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.314"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            157..408
                     /locus_tag="VYR08_002205"
     CDS             157..408
                     /locus_tag="VYR08_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467628.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSICHFVRQDLQSA"
BASE COUNT          158 a           47 c           82 g          139 t
ORIGIN      
        1 tatatgtaat taaatctaaa ttaagcttta aaaaattatt tttagcaatt tgattttgaa
       61 agtgtcatgc agcaatatga aagcgtagaa tagaggtgtt tccaatgtac aagtaagttt
      121 tgattccaaa taaagtaata gaaaaggagc tatataatga gtagtcaaat gaaagctttc
      181 ttgaatgaac ttcaaggaaa tatggaagtt gttggagaat tttttgataa tcctgaaaag
      241 gtaattagaa aatttggaat tgtcggaaga gagaaggaag cattgctaat ccgtgactta
      301 aatgatatgg atgattatgc tctttcaatt caaaatagcg ttgcatcgcc ttctggggca
      361 catagtagca tttgccattt tgttagacaa gatttacaat ccgcataatt gttaatatat
      421 aaaaca
//
LOCUS       DAVMHG010000311          424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.315, whole genome shotgun sequence.
ACCESSION   DAVMHG010000311 DAVMHG010000000
VERSION     DAVMHG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.315"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>424
                     /locus_tag="VYR08_002206"
     CDS             <1..>424
                     /locus_tag="VYR08_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV3467629.1"
                     /translation="QILVERKRDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFL
                     NPKAKERVGYPTQKPILLLEQIIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGID
                     LSEEAINITQQRLENVIKTSSNLLNKGIEAYRTKTEEEE"
BASE COUNT          173 a           57 c           76 g          118 t
ORIGIN      
        1 ccaaatacta gtggaacgaa aacgagatgg aaactctaaa actatatata aggttgataa
       61 taatggtaac tatattctag caaaagagaa aaatggagtt cccctttcag atgtttggaa
      121 tataccattt cttaatccaa aagctaaaga aagagtaggt tatcctacac aaaaacctat
      181 tctgttatta gaacaaatta taaagattgc tactgataaa aatgatatag ttttagaccc
      241 gttctgtgga agtggaacta ctttagtagc ctccaagatt ttgaatagaa attatatggg
      301 gattgattta tctgaggaag ctatcaatat aactcagcaa cgtctggaaa atgttataaa
      361 aacaagttca aatttattga ataaaggaat cgaagcatat agaaccaaaa ctgaggaaga
      421 ggaa
//
LOCUS       DAVMHG010000312          381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.317, whole genome shotgun sequence.
ACCESSION   DAVMHG010000312 DAVMHG010000000
VERSION     DAVMHG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.317"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..272
                     /locus_tag="VYR08_002207"
     CDS             <1..272
                     /locus_tag="VYR08_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467630.1"
                     /translation="YIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAI
                     ELLPFYSLPVFVTSNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
BASE COUNT          114 a           61 c           46 g          160 t
ORIGIN      
        1 cttacatttt ctatttatac aaattgaaaa gtggcttggt atttggtatt ttagtagctt
       61 tactactctt tatccctaat atattaaata cgatgatgat gaatactagt aatgatttgt
      121 ttatcaaagc aattgaactt cttccttttt attctttacc tgtatttgtg acttcaaata
      181 cgatgtctat tagtcagtat cttgtggtaa tcactacaat cattttattg tactttttca
      241 ctctcaagaa aagcaagaag tattcatttt agtttcattt agtattattt tgcataccta
      301 aaatacaata aaaaaatcag tcatcttggt atgctcctgc tttcactatt caacacgttt
      361 ttgacttata ctaggctcat t
//
LOCUS       DAVMHG010000313          380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.318, whole genome shotgun sequence.
ACCESSION   DAVMHG010000313 DAVMHG010000000
VERSION     DAVMHG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.318"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..251)
                     /locus_tag="VYR08_002208"
     CDS             complement(<1..251)
                     /locus_tag="VYR08_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467631.1"
                     /translation="MAVIHANRIISRDFSHNTISLFYNQQKNRMIYLLSNFLYAISVS
                     IIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVL"
BASE COUNT          152 a           63 c           60 g          105 t
ORIGIN      
        1 aggactaaca aaattgtatt gactacaata gctactataa aatctaatcc taaatcacct
       61 ggaataccca atttacttac tatgactagt aacacaatgc cattcaaagc ataaataatg
      121 gaaactgaga tggcatatag aaaattagac aagaggtaaa tcatccgatt tttctgttga
      181 ttataaaata aactgatcgt attgtgggaa aaatccctgc taataattcg gttagcatgt
      241 ataacagcca ttaacatacc gagcatagca taaaagtttg aaatatgctt aataccaagc
      301 gatgtccctt gagtctttag aataaagaat gtaagcaata tcggcggtat aaacgagata
      361 atcaaaccga gatatattac
//
LOCUS       DAVMHG010000314          367 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.319, whole genome shotgun sequence.
ACCESSION   DAVMHG010000314 DAVMHG010000000
VERSION     DAVMHG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.319"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>367
                     /locus_tag="VYR08_002209"
     CDS             <1..>367
                     /locus_tag="VYR08_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467632.1"
                     /translation="FAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWEKEDSFESDL
                     TNTLKDKIQKSDLSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCTGL
                     VELLLYEMSISKNHNWSEK"
BASE COUNT          127 a           54 c           58 g          128 t
ORIGIN      
        1 cttttgcaat catcatgctt actacaatat ttttctggac tataatggat atcaatcatc
       61 tagtttttaa taagacggaa tttcttttta atcaaaataa atgggaaaaa gaagattcgt
      121 ttgaaagtga tttgacaaat acgttaaagg ataaaataca aaaatcagat ctcagttatt
      181 ctgacttaat gtctcttctt ctctatgatg cgatgaacca atccaccttt ttaacggata
      241 gtgaactttt tgaggatatt ttgaaaaaaa tcgaagattc tcataacacc cttctgtgta
      301 caggtcttgt cgagttactg ctgtatgaaa tgtctattag taagaatcat aattggtcag
      361 aaaagat
//
LOCUS       DAVMHG010000315          357 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.320, whole genome shotgun sequence.
ACCESSION   DAVMHG010000315 DAVMHG010000000
VERSION     DAVMHG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.320"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..355)
                     /locus_tag="VYR08_002210"
     CDS             complement(<1..355)
                     /locus_tag="VYR08_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV3467633.1"
                     /translation="MGEQQRIASELDLLSKLILRRQEQLEELNLLVKSRFNEMFGDPL
                     NNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIG
                     RVGAYCGNVRTTHGKV"
BASE COUNT          109 a           79 c           45 g          124 t
ORIGIN      
        1 ataccttacc gtgggtagtt cttacattac cacaatatgc acctacacga ccaatgatga
       61 tagtaggatt atcaattaaa tattttcgac ttttccaagc aatgccattt ccaccatagg
      121 caggataacc ttcaaaaact ctatcatgtt tatcaagaaa ttttccactt gaaaatttaa
      181 aacactgttg tcccgttttt actgcaaatt tcttgttatt atttaaagga tccccaaaca
      241 tctcgttaaa tcgggatttt acaagtagat taagctcttc aagttgttct tgtctcctta
      301 aaatcagctt gcttaacaaa tccagctcac ttgcaattct ttgttgctct cccaaat
//
LOCUS       DAVMHG010000316          356 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.321, whole genome shotgun sequence.
ACCESSION   DAVMHG010000316 DAVMHG010000000
VERSION     DAVMHG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.321"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..206)
                     /locus_tag="VYR08_002211"
     CDS             complement(<1..206)
                     /locus_tag="VYR08_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467634.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYL"
BASE COUNT          136 a           57 c           54 g          109 t
ORIGIN      
        1 ttaagataaa tcccatcaat ccaacccaag tggaaaaaac cgatataggt cagataaaaa
       61 atataaagtt gccagtaact gtactctgtt acccccatta atttggggaa aattaagaca
      121 accaaggaag aggtcaagac tgtcaaaaga tttgaagaaa tgacataaga gaaattagtt
      181 agtatatttt taattttgct attcatcaac tatcctctct atttttattg ttttcagttt
      241 tccgatttaa tacttgtgtc agagagttta attactagtt ttcctctgtg gaaatccaaa
      301 ttccaataga agaaagccat taggtaaacc aagactataa atacaagtgt aaacaa
//
LOCUS       DAVMHG010000317          352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.322, whole genome shotgun sequence.
ACCESSION   DAVMHG010000317 DAVMHG010000000
VERSION     DAVMHG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.322"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>352)
                     /locus_tag="VYR08_002212"
     CDS             complement(<1..>352)
                     /locus_tag="VYR08_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467635.1"
                     /translation="FRNNFMFIVSKWNLLYRKPYIKSISAAITGAILLISSFQMISQN
                     ILSQFQDDSDLELKVAFIVYVGAPILIVLANIISIAFLSSHQERIEIQQFEILGTSNY
                     QMVKIKVGEAIFLTF"
BASE COUNT          135 a           58 c           44 g          115 t
ORIGIN      
        1 aaatgtcaaa aatatagcct caccaacttt aatcttaacc atctggtaat tagaagttcc
       61 taaaatttca aattgctgaa tctcaatcct ttcttgatgc gatgacaaaa atgcaattga
      121 aataatattt gcaagtacta tcaaaattgg tgctcctaca tagacaataa atgccacttt
      181 tagctctaaa tcactgtcat cttgaaattg agatagtata ttctgagaaa tcatttgaaa
      241 actagaaatt agtaatatag ctcctgtaat tgcagcactg atagatttta tataaggctt
      301 acgatatagt aaattccact tcgaaacaat gaacataaaa ttatttctaa at
//
LOCUS       DAVMHG010000318          351 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.323, whole genome shotgun sequence.
ACCESSION   DAVMHG010000318 DAVMHG010000000
VERSION     DAVMHG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.323"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>351
                     /locus_tag="VYR08_002213"
     CDS             <1..>351
                     /locus_tag="VYR08_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV3467636.1"
                     /translation="RLEEIKTDREEAKRKADAKLKEAVEKNAANSEQGEPKRRVKRGV
                     LGEPATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVAEAEKKVEEAKKKAEDQKEED
                     RRNYPTNTYKTLEL"
BASE COUNT          148 a           60 c           87 g           56 t
ORIGIN      
        1 aaggttagaa gaaatcaaga cagatcgtga agaagctaaa cgaaaagcag atgctaagtt
       61 gaaggaagct gttgaaaaga atgcagcgaa ttcagagcaa ggtgaaccaa agaggcgggt
      121 aaaacgagga gttcttggag agccagcaac acctgataaa aaagaaaatg atgcgaagtc
      181 ttcagattct agcgtaggtg aagaaactct tccaagccca tccctgaaac cagaaaaaaa
      241 ggttgcagaa gctgagaaga aggttgaaga agctaagaaa aaagccgagg atcaaaaaga
      301 agaagatcgc cgtaactacc caaccaatac ttacaaaacg cttgaacttg a
//
LOCUS       DAVMHG010000319          346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.324, whole genome shotgun sequence.
ACCESSION   DAVMHG010000319 DAVMHG010000000
VERSION     DAVMHG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.324"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>346
                     /locus_tag="VYR08_002214"
     CDS             <1..>346
                     /locus_tag="VYR08_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467637.1"
                     /translation="SASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAST
                     SASTSASTSASAS"
BASE COUNT           78 a          118 c           79 g           71 t
ORIGIN      
        1 tcagcttccg catcaacgag tgcttcggct tcagcctcaa cgagtgcctc agcttcagcg
       61 tcaaccagtg cttcagcctc agcctcaacg agtgcttcgg cttcagcaag taccagtgcc
      121 tcagcctcag caagtaccag tgcttcggct tcagcaagta ctagcgcgtc agcttcagca
      181 agcaccagcg cctcagcctc agcaagtacc agtgcgtcag cttcagcgtc aaccagtgcg
      241 tcagcttcag caagcaccag tgcttcggct tccgcatcaa caagtgcgtc agcttcagcg
      301 tcaacgagcg catcaacctc agcaagtact agtgcctcag cttcag
//
LOCUS       DAVMHG010000320          343 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.325, whole genome shotgun sequence.
ACCESSION   DAVMHG010000320 DAVMHG010000000
VERSION     DAVMHG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.325"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..306
                     /locus_tag="VYR08_002215"
     CDS             <1..306
                     /locus_tag="VYR08_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV3467638.1"
                     /translation="PEKPKPEVKPQPEKPKPEVKPQPEKPKPDNSKPQADDKKPSTTN
                     NLSKDKQPSNQASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
BASE COUNT          146 a           74 c           58 g           65 t
ORIGIN      
        1 ccagaaaaac caaaaccaga ggttaaaccg cagccggaaa aaccaaaacc agaggttaaa
       61 ccgcagccgg aaaaaccaaa accagataat agcaagccac aagcagatga taagaagcca
      121 tcaactacaa ataatttaag caaggacaag caaccttcta accaagcttc aacaaacgaa
      181 aaagcaacaa ataaaccgaa gaagtcattg ccatcaactg gatctatttc aaatctagca
      241 cttgaaattg caggtcttct taccttggcg ggggcaacca ttcttgctaa gaaaagaatg
      301 aaatagtgat tgattcattc aaaaatgttt gtaaaaagta ttc
//
LOCUS       DAVMHG010000321          338 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.326, whole genome shotgun sequence.
ACCESSION   DAVMHG010000321 DAVMHG010000000
VERSION     DAVMHG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.326"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..316)
                     /locus_tag="VYR08_002216"
     CDS             complement(<1..316)
                     /locus_tag="VYR08_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV3467639.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVL
                     KKV"
BASE COUNT          112 a           54 c           43 g          129 t
ORIGIN      
        1 ttaccttttt taacacctca attatctgat catctgaaaa atattcattt tgaaataaag
       61 ttacgttctc acgaatagta gtgtcaaaaa tatatggtgt ctgatcaact gttggtattg
      121 aatctgaact ctttttccca tgtgataaca aatttacata acctttttgt ggctttaaag
      181 aaccattaat taaatttaaa atcgttgttt tcccactacc agaagttcct gttaataata
      241 ccctaaatgg tgacttaaat gagaagtcaa tacttaattt attttctggt gtaatagaat
      301 atacaacatc tttcatgtgt atctcatcta ttgatgaa
//
LOCUS       DAVMHG010000322          325 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.327, whole genome shotgun sequence.
ACCESSION   DAVMHG010000322 DAVMHG010000000
VERSION     DAVMHG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.327"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          113 a           46 c           44 g          122 t
ORIGIN      
        1 tatatcttat ttcagtttac tatactagct tattattcta gtgaaaaatt gaatgagcca
       61 ctatttattt aatatgtatg atgagggaat gactcaaaaa cttcttgtcg atataagaat
      121 atctgagata gtattttaaa acgaaaggat tataaaagct catttccttc gcacttattt
      181 tatagagatc tatcattata ctctttagaa atcaaaaagc aacacacctt gtcatcgttt
      241 tctcgtttgc tctttgattt taattgggcc ttagtaaata ctactcttaa attaaactat
      301 agtgaattga aataagatgt gaaca
//
LOCUS       DAVMHG010000323          311 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.328, whole genome shotgun sequence.
ACCESSION   DAVMHG010000323 DAVMHG010000000
VERSION     DAVMHG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.328"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..311
                     /locus_tag="VYR08_002217"
                     /pseudo
     CDS             17..311
                     /locus_tag="VYR08_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          116 a           55 c           58 g           82 t
ORIGIN      
        1 aattgattga tcgttaatgt tggaacaggc attcaaagag aagcactacg agaatacgat
       61 tctccatagt gaccaaggct ggcaatacca acacgattct tatcatcgct tcctagagag
      121 taagggaatt caagcatcta tgtcacgcaa gggaaacagc caagacaacg gtaggatgga
      181 atctttcttt ggcattttaa aatccgaaat gttttatggc tatgagaaaa catttaaatc
      241 acttaatcaa ttggaacaag ccattataga ctatattgat tactacaaca ataaacgaat
      301 taaggtaaaa c
//
LOCUS       DAVMHG010000324          304 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.330, whole genome shotgun sequence.
ACCESSION   DAVMHG010000324 DAVMHG010000000
VERSION     DAVMHG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.330"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            72..>304
                     /locus_tag="VYR08_002218"
     CDS             72..>304
                     /locus_tag="VYR08_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467640.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPF"
BASE COUNT           94 a           41 c           45 g          124 t
ORIGIN      
        1 tttttttgat tttaatcata gcaatgatga tttatccaaa attaagaaaa tgataaggac
       61 actgtaaaat tatgatacgt aaactaattg ttttcttact aatgctccct ctttttgtct
      121 tgtggctaga gttgcatgtc ctagtgaata acttactatt gaatctagaa tctccccttg
      181 attttgtgat tagtatgagt cttgctttct gtagtttgat tttatcaaaa atagttttag
      241 acatactata cgcattgaaa gatttataca agaaagaagg tcttattaag atttttccct
      301 ttat
//
LOCUS       DAVMHG010000325          298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.331, whole genome shotgun sequence.
ACCESSION   DAVMHG010000325 DAVMHG010000000
VERSION     DAVMHG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.331"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..149)
                     /locus_tag="VYR08_002219"
     CDS             complement(<1..149)
                     /locus_tag="VYR08_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV3467641.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEK"
BASE COUNT           95 a           57 c           25 g          121 t
ORIGIN      
        1 tctttttcaa ttggtaaaaa atattcgtat ttcaagttac ctttttgatt tctaatttct
       61 tcaacaaggc cactatcaag catttctctt gcaaacttta ttgcactatc tccatcacct
      121 ttataatata catgaatagt caatgtcatc ttatatcctc caaaatcatc cttcaatttt
      181 aaaaaaacaa gtttagatga ggatctaaac ttgtttttta tgaactaatt atctaacgtt
      241 tcgccattac tttcaatcac ttctttatac caataaaatg attttttctt atagcgat
//
LOCUS       DAVMHG010000326          298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.332, whole genome shotgun sequence.
ACCESSION   DAVMHG010000326 DAVMHG010000000
VERSION     DAVMHG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.332"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          107 a           53 c           37 g          101 t
ORIGIN      
        1 ttcttctctc cacttaagta ttttaaaaac ggtttcctta aatctttggt tgtatttatc
       61 ttatttaatg aataggcaat tgattgtatc ggccctaaaa ctttatcgtt tgctaagaaa
      121 atacctatca gttcactaaa agaaaggctt ttatgataaa ttacaaaata acatcctaca
      181 accaagggaa ctagaaagca aaaacctgaa attagtactg caaccaattt tgaaagaacc
      241 tctgatcgtt tcaaattaaa agtagaatct tctagtttat ccaacttttt atccgaca
//
LOCUS       DAVMHG010000327          290 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.334, whole genome shotgun sequence.
ACCESSION   DAVMHG010000327 DAVMHG010000000
VERSION     DAVMHG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.334"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..221)
                     /locus_tag="VYR08_002220"
     CDS             complement(<1..221)
                     /locus_tag="VYR08_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HEV3467642.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYIT"
BASE COUNT          100 a           52 c           34 g          104 t
ORIGIN      
        1 gtgatgtaca ccaactccaa aggaacgtta ataaacatct ctgtaaaacg ggtcatatta
       61 tctctatttt tgagaaaaaa acttttctca atatcaattg gatttacttt atctccaatt
      121 tttcttccaa aagctattcc ccttccaaaa agaagaatat cgatatcttt tttactttgc
      181 gcaattacgg cattgtggtt tagtattcgt tttatgatca taaaatttcc cttttaaaat
      241 aaaaggaacc tacttaaaaa acatcaagaa acttcttctg atgtgattaa
//
LOCUS       DAVMHG010000328          288 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.335, whole genome shotgun sequence.
ACCESSION   DAVMHG010000328 DAVMHG010000000
VERSION     DAVMHG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.335"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           90 a           46 c           50 g          102 t
ORIGIN      
        1 tcattatggg acatttgttt aaccttcttt catgattttt gtggtgaatt gattgtataa
       61 cgaggggaca gcaaatgtcc tcttttttgt ataaaaaaat ctggcatgga atctctatcc
      121 ataccagaaa gtgtataccc caaaaaaata actcaaatac aaattcattg aatatagaga
      181 ggagaacatt tttatgaatt ttggacaaaa cctttataac tggtttctat caaacgctca
      241 atcactggtg cttttagcaa tcgttgtgat tggcttgtat cttggctt
//
LOCUS       DAVMHG010000329          282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.336, whole genome shotgun sequence.
ACCESSION   DAVMHG010000329 DAVMHG010000000
VERSION     DAVMHG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.336"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..106)
                     /locus_tag="VYR08_002221"
     CDS             complement(<1..106)
                     /locus_tag="VYR08_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV3467643.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGLI"
     gene            complement(103..>282)
                     /locus_tag="VYR08_002222"
     CDS             complement(103..>282)
                     /locus_tag="VYR08_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV3467644.1"
                     /translation="NFRFKVHIEDNDSFTIEVPIEENILDVINEVGKACEIKNFSTSK
                     LTLQESYLQRIGGEK"
BASE COUNT           86 a           53 c           27 g          116 t
ORIGIN      
        1 taattaaacc tttacttcta aaaaaagcaa taatctcatt tttagcaata atcgctagac
       61 ctcttaaacc taagtccgat ttgtgcctta attgatcagc cttcattttt ctcctcctat
      121 tctttgcaaa taactttctt gtaaggttaa tttacttgtt gaaaagtttt taatttcaca
      181 tgcttttcct acctcattga taacatctaa gatattttct tctataggga cttctattgt
      241 aaaactatca ttatcttcga tgtgaacttt aaatctaaaa tt
//
LOCUS       DAVMHG010000330          261 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.denovo.338, whole genome shotgun sequence.
ACCESSION   DAVMHG010000330 DAVMHG010000000
VERSION     DAVMHG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.denovo.338"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           72 a           42 c           42 g          105 t
ORIGIN      
        1 cgtttatgat atttagctgt acgagagtct tttaaaagtg ttttgatggt ttggatttct
       61 tctttaatcg atttcatatt actattgtat aatgcttttt gattttagtc tagtattata
      121 ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg tcgtatagat gttactgact
      181 tcgtcagttc tatctacaac ctaaaaacag tgttttgagc agtctgtagc taactttcta
      241 tagtaaaatg aaataagaac a
//
LOCUS       DAVMHG010000331         7806 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.guided.099, whole genome shotgun sequence.
ACCESSION   DAVMHG010000331 DAVMHG010000000
VERSION     DAVMHG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.guided.099"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            138..254
                     /locus_tag="VYR08_002223"
     CDS             138..254
                     /locus_tag="VYR08_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV3467645.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            270..2189
                     /gene="tet(M)"
                     /locus_tag="VYR08_002224"
     CDS             270..2189
                     /gene="tet(M)"
                     /locus_tag="VYR08_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV3467646.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDYLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLLRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2308..2475
                     /locus_tag="VYR08_002225"
     CDS             2308..2475
                     /locus_tag="VYR08_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV3467647.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2535..2888)
                     /locus_tag="VYR08_002226"
     CDS             complement(2535..2888)
                     /locus_tag="VYR08_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467648.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3393..3815
                     /locus_tag="VYR08_002227"
     CDS             3393..3815
                     /locus_tag="VYR08_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3467649.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3812..4042
                     /locus_tag="VYR08_002228"
     CDS             3812..4042
                     /locus_tag="VYR08_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467650.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4503..4706
                     /locus_tag="VYR08_002229"
     CDS             4503..4706
                     /locus_tag="VYR08_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV3467651.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4788..6005
                     /locus_tag="VYR08_002230"
     CDS             4788..6005
                     /locus_tag="VYR08_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3467652.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYND
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMSEMK
                     RLNKEKQQERLVA"
     gene            6242..6403
                     /locus_tag="VYR08_002231"
     CDS             6242..6403
                     /locus_tag="VYR08_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467653.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            6390..6596
                     /locus_tag="VYR08_002232"
     CDS             6390..6596
                     /locus_tag="VYR08_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467654.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            6642..7001
                     /locus_tag="VYR08_002233"
     CDS             6642..7001
                     /locus_tag="VYR08_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467655.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMASG"
     gene            7130..7555
                     /locus_tag="VYR08_002234"
     CDS             7130..7555
                     /locus_tag="VYR08_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467656.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
BASE COUNT         2739 a         1176 c         1569 g         2322 t
ORIGIN      
        1 tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat
       61 agtaggaaca aataattgga tgtccttttt taggagggct tagttttttg tacccagttt
      121 aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc
      181 ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc
      241 ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc
      301 atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta
      361 cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc
      421 agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga
      481 acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag
      541 ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta
      601 tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg
      661 accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg
      721 aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg
      781 aatctgaaca atgggatacg gtaatagagg gaaacgatta ccttttagag aaatatatgt
      841 ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt
      901 gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta
      961 tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg
     1021 gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt
     1081 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
     1141 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
     1201 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
     1261 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
     1321 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
     1381 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
     1441 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
     1501 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
     1561 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
     1621 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
     1681 aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa ggattgtatg
     1741 gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta
     1801 gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag
     1861 ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc
     1921 ttttacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga
     1981 aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta
     2041 gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc
     2101 atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag
     2161 tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt
     2221 cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag
     2281 atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta
     2341 aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa
     2401 gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag
     2461 acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt
     2521 atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat
     2581 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt
     2641 cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt
     2701 aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta
     2761 gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt
     2821 ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt
     2881 ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat
     2941 agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca
     3001 ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc
     3061 caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc
     3121 cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca
     3181 atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc
     3241 tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc
     3301 aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac
     3361 ttcctttcct agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa
     3421 tagaaaatca gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata
     3481 tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc
     3541 ttgttagcca gtttgcgaca acagataact attcaactga ctttcagatt tttacactca
     3601 atgggttatc agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag
     3661 acaagaaacg tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg
     3721 cagacctgtt gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct
     3781 taattaaaaa gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt
     3841 tcctctcatt gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca
     3901 ttacagaggg tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca
     3961 aagtatggtc gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat
     4021 tttgtcattt gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc
     4081 cacgcttccc gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt
     4141 tttgataggc taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt
     4201 cgataagaaa agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg
     4261 attcatgtta gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat
     4321 cgtgataatg atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa
     4381 tgagtatggc tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg
     4441 ttcgattgct ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt
     4501 ttatgaagca gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt
     4561 caaaatattt tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg
     4621 caaattggct gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa
     4681 ttatagatac attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt
     4741 attaagtcgt atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa
     4801 gacgtgacaa taaaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat
     4861 acttatacaa atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg
     4921 tggctacaga ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg
     4981 cagagttaca gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct
     5041 gccagcttta cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg
     5101 gacgcaaata tcttatggat attttgaaga aagacaagtt aggtgtaaga agtattgaca
     5161 gtattaagcc atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt
     5221 atcaaaccat caataactac aaacgttctt taaaggcttc attctatatt gctatacaag
     5281 atgattgtgt tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata
     5341 ctgtccctaa gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag
     5401 ctgataaaac ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc
     5461 gtatttcaga gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca
     5521 atatagacca tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga
     5581 ccaaaagtgg cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag
     5641 tgttagcgaa tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct
     5701 ttcttaatag aaagaactat ccaaaagtgg cgagtgacta taatggtatg atgaaaggtc
     5761 ttgttaagaa atacaacaag tataatgatg ataagttacc acacatcact ccacatagtt
     5821 tgcgacatac attctgtacc aactatgcaa atgcaggaat gaacccaaaa gcattacaat
     5881 acattatggg gcatgcaaat atagccatga cgctgaacta ttacgcacac gcaacatttg
     5941 attccgcaat gtcagagatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg
     6001 cttagtagta caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga
     6061 aatatgcaaa gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat
     6121 aaggctttac gagcatttaa gaaaatataa aaagataatt agaaatttat actttgttta
     6181 tatataaaaa ttatgacaag agtgtgctat tctttttatg agaggtgtat gaatatgata
     6241 aatgtatgtg ataaatgtat gtgatgttgg aaaaagaata aaagaactta gaatatcttc
     6301 aaatcttact caagataaga ttgctgagta tttgtctttg aatcaaagca tgattgccaa
     6361 aatggaaaaa ggtgaaagga atatcacgaa tggatttaag taataaagct tcaaatctta
     6421 gaaaaaagtt gggagctgat ggtgaatcgc cgatagatat ttttaaattg gtacaaaaga
     6481 tagaaaattt gacgctggta ttttatggac tcggaaagaa tattagcgga gtctgttata
     6541 aaggaactca gttcagtctc attgcagtca attcagacat gccattagga aggtaaagat
     6601 tttctttagc acatgaactg tatcatcttt attatgatga ggtgaagaag agttcagtca
     6661 gtcttatctt gattggtgaa ggagatgaaa ctgaaagaaa agcggatcag tttgcttctt
     6721 attttttaat tttcccatct tcactgtata ggatggttga ggaaatcaga gaaaatgcca
     6781 atagaactca tcttgaagta gaagatatta taaaattggg tcagttttat ggtatcagtc
     6841 ataaagctat gttatataga ttgaggaatg atggatacct tgatgcagaa gaaattaaaa
     6901 atatggatat tagtgttata gagacagctt caagattagg ctatgataca agtttatatc
     6961 gtcctttgtc agaaagtaaa aaagaaatgg catcaggata atatattaat tcaactgaac
     7021 aacttttaga aaataacaga atttcgcaag ggaagtatga ggaactgtta ctagatgctt
     7081 tcagatatga tattgtatat gggctagatg aagagggggg agttgtcgtt tgactagtcg
     7141 tgtatttatt gatgcagatt gtatttcagt atttttatgg gttggcactg aacatctttt
     7201 agaaaagctc tatttgggta aaattgttat tccacaagag gtgtatgatg aaatcaatat
     7261 acctacaatt ccccatttaa aatctaggat agaccagttg gtaactaagg gttcagctga
     7321 gattgtgagc atagacattg gaactgaaga atacgcatta tatagagatt taacaagaaa
     7381 tcatgatagt aacaagatta ttggtaaggg agaagcgact tctattttct tagcgaaaaa
     7441 gcataatggg atattaggaa gtaataacct aagagatgtt aaaccatatg tagaagaatt
     7501 ttctttagaa catatgacaa caggagatat actgattgaa gcgtttaaag cgtaatttat
     7561 tactgaataa gagggcaatc atatctggaa taatatgctt aaaaagagaa ggaaaattgg
     7621 tgcaaattca ttttcagact atcttcgtgg aagtattcat caaaatagac aaaaataaat
     7681 ttggataaat cgaactcact attcaggagg catatgagca attcgaaaaa gaaaagtgtc
     7741 aaattgagcc tataggagta gaagtgaaat agtaagtcct gcatagtgga tgagagaaaa
     7801 gttctc
//
LOCUS       DAVMHG010000332         7806 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.guided.100, whole genome shotgun sequence.
ACCESSION   DAVMHG010000332 DAVMHG010000000
VERSION     DAVMHG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.guided.100"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            138..254
                     /locus_tag="VYR08_002235"
     CDS             138..254
                     /locus_tag="VYR08_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV3467657.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            270..2189
                     /gene="tet(M)"
                     /locus_tag="VYR08_002236"
     CDS             270..2189
                     /gene="tet(M)"
                     /locus_tag="VYR08_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV3467658.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGILTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDYLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLLRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2308..2475
                     /locus_tag="VYR08_002237"
     CDS             2308..2475
                     /locus_tag="VYR08_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV3467659.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2535..2888)
                     /locus_tag="VYR08_002238"
     CDS             complement(2535..2888)
                     /locus_tag="VYR08_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467660.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3393..3815
                     /locus_tag="VYR08_002239"
     CDS             3393..3815
                     /locus_tag="VYR08_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV3467661.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3812..4042
                     /locus_tag="VYR08_002240"
     CDS             3812..4042
                     /locus_tag="VYR08_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV3467662.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4503..4706
                     /locus_tag="VYR08_002241"
     CDS             4503..4706
                     /locus_tag="VYR08_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV3467663.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4788..6005
                     /locus_tag="VYR08_002242"
     CDS             4788..6005
                     /locus_tag="VYR08_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV3467664.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYND
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMSEMK
                     RLNKEKQQERLVA"
     gene            6242..6403
                     /locus_tag="VYR08_002243"
     CDS             6242..6403
                     /locus_tag="VYR08_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV3467665.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            6390..6596
                     /locus_tag="VYR08_002244"
     CDS             6390..6596
                     /locus_tag="VYR08_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467666.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            6642..7001
                     /locus_tag="VYR08_002245"
     CDS             6642..7001
                     /locus_tag="VYR08_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467667.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMASG"
     gene            7130..7555
                     /locus_tag="VYR08_002246"
     CDS             7130..7555
                     /locus_tag="VYR08_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV3467668.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
BASE COUNT         2739 a         1175 c         1569 g         2323 t
ORIGIN      
        1 tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat
       61 agtaggaaca aataattgga tgtccttttt taggagggct tagttttttg tacccagttt
      121 aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc
      181 ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc
      241 ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc
      301 atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta
      361 cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc
      421 agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga
      481 acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag
      541 ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta
      601 tattatttca tgcacttagg aaaatgggga ttctcacaat cttttttatc aataagattg
      661 accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg
      721 aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg
      781 aatctgaaca atgggatacg gtaatagagg gaaacgatta ccttttagag aaatatatgt
      841 ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt
      901 gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta
      961 tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg
     1021 gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt
     1081 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
     1141 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
     1201 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
     1261 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
     1321 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
     1381 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
     1441 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
     1501 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
     1561 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
     1621 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
     1681 aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa ggattgtatg
     1741 gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta
     1801 gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag
     1861 ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc
     1921 ttttacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga
     1981 aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta
     2041 gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc
     2101 atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag
     2161 tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt
     2221 cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag
     2281 atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta
     2341 aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa
     2401 gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag
     2461 acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt
     2521 atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat
     2581 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt
     2641 cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt
     2701 aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta
     2761 gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt
     2821 ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt
     2881 ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat
     2941 agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca
     3001 ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc
     3061 caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc
     3121 cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca
     3181 atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc
     3241 tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc
     3301 aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac
     3361 ttcctttcct agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa
     3421 tagaaaatca gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata
     3481 tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc
     3541 ttgttagcca gtttgcgaca acagataact attcaactga ctttcagatt tttacactca
     3601 atgggttatc agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag
     3661 acaagaaacg tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg
     3721 cagacctgtt gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct
     3781 taattaaaaa gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt
     3841 tcctctcatt gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca
     3901 ttacagaggg tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca
     3961 aagtatggtc gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat
     4021 tttgtcattt gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc
     4081 cacgcttccc gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt
     4141 tttgataggc taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt
     4201 cgataagaaa agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg
     4261 attcatgtta gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat
     4321 cgtgataatg atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa
     4381 tgagtatggc tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg
     4441 ttcgattgct ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt
     4501 ttatgaagca gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt
     4561 caaaatattt tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg
     4621 caaattggct gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa
     4681 ttatagatac attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt
     4741 attaagtcgt atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa
     4801 gacgtgacaa taaaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat
     4861 acttatacaa atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg
     4921 tggctacaga ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg
     4981 cagagttaca gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct
     5041 gccagcttta cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg
     5101 gacgcaaata tcttatggat attttgaaga aagacaagtt aggtgtaaga agtattgaca
     5161 gtattaagcc atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt
     5221 atcaaaccat caataactac aaacgttctt taaaggcttc attctatatt gctatacaag
     5281 atgattgtgt tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata
     5341 ctgtccctaa gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag
     5401 ctgataaaac ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc
     5461 gtatttcaga gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca
     5521 atatagacca tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga
     5581 ccaaaagtgg cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag
     5641 tgttagcgaa tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct
     5701 ttcttaatag aaagaactat ccaaaagtgg cgagtgacta taatggtatg atgaaaggtc
     5761 ttgttaagaa atacaacaag tataatgatg ataagttacc acacatcact ccacatagtt
     5821 tgcgacatac attctgtacc aactatgcaa atgcaggaat gaacccaaaa gcattacaat
     5881 acattatggg gcatgcaaat atagccatga cgctgaacta ttacgcacac gcaacatttg
     5941 attccgcaat gtcagagatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg
     6001 cttagtagta caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga
     6061 aatatgcaaa gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat
     6121 aaggctttac gagcatttaa gaaaatataa aaagataatt agaaatttat actttgttta
     6181 tatataaaaa ttatgacaag agtgtgctat tctttttatg agaggtgtat gaatatgata
     6241 aatgtatgtg ataaatgtat gtgatgttgg aaaaagaata aaagaactta gaatatcttc
     6301 aaatcttact caagataaga ttgctgagta tttgtctttg aatcaaagca tgattgccaa
     6361 aatggaaaaa ggtgaaagga atatcacgaa tggatttaag taataaagct tcaaatctta
     6421 gaaaaaagtt gggagctgat ggtgaatcgc cgatagatat ttttaaattg gtacaaaaga
     6481 tagaaaattt gacgctggta ttttatggac tcggaaagaa tattagcgga gtctgttata
     6541 aaggaactca gttcagtctc attgcagtca attcagacat gccattagga aggtaaagat
     6601 tttctttagc acatgaactg tatcatcttt attatgatga ggtgaagaag agttcagtca
     6661 gtcttatctt gattggtgaa ggagatgaaa ctgaaagaaa agcggatcag tttgcttctt
     6721 attttttaat tttcccatct tcactgtata ggatggttga ggaaatcaga gaaaatgcca
     6781 atagaactca tcttgaagta gaagatatta taaaattggg tcagttttat ggtatcagtc
     6841 ataaagctat gttatataga ttgaggaatg atggatacct tgatgcagaa gaaattaaaa
     6901 atatggatat tagtgttata gagacagctt caagattagg ctatgataca agtttatatc
     6961 gtcctttgtc agaaagtaaa aaagaaatgg catcaggata atatattaat tcaactgaac
     7021 aacttttaga aaataacaga atttcgcaag ggaagtatga ggaactgtta ctagatgctt
     7081 tcagatatga tattgtatat gggctagatg aagagggggg agttgtcgtt tgactagtcg
     7141 tgtatttatt gatgcagatt gtatttcagt atttttatgg gttggcactg aacatctttt
     7201 agaaaagctc tatttgggta aaattgttat tccacaagag gtgtatgatg aaatcaatat
     7261 acctacaatt ccccatttaa aatctaggat agaccagttg gtaactaagg gttcagctga
     7321 gattgtgagc atagacattg gaactgaaga atacgcatta tatagagatt taacaagaaa
     7381 tcatgatagt aacaagatta ttggtaaggg agaagcgact tctattttct tagcgaaaaa
     7441 gcataatggg atattaggaa gtaataacct aagagatgtt aaaccatatg tagaagaatt
     7501 ttctttagaa catatgacaa caggagatat actgattgaa gcgtttaaag cgtaatttat
     7561 tactgaataa gagggcaatc atatctggaa taatatgctt aaaaagagaa ggaaaattgg
     7621 tgcaaattca ttttcagact atcttcgtgg aagtattcat caaaatagac aaaaataaat
     7681 ttggataaat cgaactcact attcaggagg catatgagca attcgaaaaa gaaaagtgtc
     7741 aaattgagcc tataggagta gaagtgaaat agtaagtcct gcatagtgga tgagagaaaa
     7801 gttctc
//
LOCUS       DAVMHG010000333         2226 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.guided.186, whole genome shotgun sequence.
ACCESSION   DAVMHG010000333 DAVMHG010000000
VERSION     DAVMHG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.guided.186"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..587
                     /locus_tag="VYR08_002247"
                     /pseudo
     CDS             33..587
                     /locus_tag="VYR08_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            590..769
                     /locus_tag="VYR08_002248"
                     /pseudo
     CDS             590..769
                     /locus_tag="VYR08_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            862..1656
                     /locus_tag="VYR08_002249"
                     /note="aph(3')-IIIa"
     CDS             862..1656
                     /locus_tag="VYR08_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEV3467669.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1932..2226
                     /locus_tag="VYR08_002250"
                     /pseudo
     CDS             1932..2226
                     /locus_tag="VYR08_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT          752 a          378 c          479 g          617 t
ORIGIN      
        1 tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa tattctcaaa
       61 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      121 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      181 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      241 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      301 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      361 ttaaaaaagt ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt gaagaaggat
      421 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      481 cgattaagca attgcttaag ctgccagctg aatgctttca tcctaaacca aaagtaaaca
      541 gtgccttaat aaaacttacc cgccatacca cagatgttcc agataaataa tagaatgggc
      601 aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg
      661 taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
      721 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
      781 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
      841 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
      901 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
      961 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     1021 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     1081 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     1141 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     1201 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     1261 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     1321 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
     1381 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     1441 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     1501 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
     1561 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     1621 ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga tgtctaaaaa
     1681 gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg caactgtcca
     1741 tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat attagagatg
     1801 taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt gcaatgagaa
     1861 gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa gtcacagagg
     1921 aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag ctgcaatcaa
     1981 aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt aaggacagtt
     2041 tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga ggccggaaaa
     2101 ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc ggcggaagtg
     2161 ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc atttataaag
     2221 gatttt
//
LOCUS       DAVMHG010000334         1079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_J08
            SAMN29867933-rid17924463.guided.240, whole genome shotgun sequence.
ACCESSION   DAVMHG010000334 DAVMHG010000000
VERSION     DAVMHG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867933
            Sequence Read Archive: SRR20340382
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001719887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001719887.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:16:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,251
            CDSs (total)                      :: 2,206
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 70 of 137
            Pseudo Genes (incomplete)         :: 63 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867933-rid17924463.guided.240"
                     /isolate="GC089790_J08"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2010-01-15"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..770
                     /gene="erm(B)"
                     /locus_tag="VYR08_002251"
     CDS             33..770
                     /gene="erm(B)"
                     /locus_tag="VYR08_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV3467670.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          387 a          184 c          193 g          315 t
ORIGIN      
        1 tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa tattctcaaa
       61 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      121 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      181 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      241 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      301 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      361 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      421 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      481 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
      541 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      601 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      661 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
      721 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
      781 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
      841 atactactga cagcttccaa ggagctaaag aggtccctag cgcctacggg gaatttgtat
      901 cgataagggg tacaaattcc cactaagcgc tagggacccc ttgtaggaaa atgtcctaag
      961 tgggatatct gtcaactggt atggttgact aaaaatactt cctacgaaaa tgtagggggt
     1021 atttttttac gaaaaaatac ggctctttgt caactgtagt gggttgaaga aaagctaag
//
